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<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R037</accession>
    <name>A0A1E5R037_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">L15</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">YL23</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g4652</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000017">
        <property type="protein sequence ID" value="OEJ80244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R037"/>
    <dbReference type="FunCoup" id="A0A1E5R037">
        <property type="interactions" value="1064"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R037"/>
    <dbReference type="InParanoid" id="A0A1E5R037"/>
    <dbReference type="OrthoDB" id="1478556at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.250:FF:000002">
        <property type="entry name" value="60S ribosomal protein L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1550.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="entry name" value="Ribosomal protein L11/L12, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_uL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_uL11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020785">
        <property type="entry name" value="Ribosomal_uL11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_uL11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_uL11_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="Ribosomal L11/L12e N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00359">
        <property type="entry name" value="RIBOSOMAL_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8 11" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL11 N-terminal" evidence="10">
        <location>
            <begin position="14"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL11 C-terminal" evidence="9">
        <location>
            <begin position="74"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00064933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00080111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00082757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ80244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="165" mass="17826" checksum="23B0813E72DA1C76" modified="2017-01-18" version="1">MPPKFDPTEVKYLYLRAVGGEVGASAALAPKIGPLGLSPKKVGEDIAKATKNFKGIKVTVQLKIQNRQATASVVPSASSLVITALKEPPRDRKKDKNVKHSGNLQLEDIIEVARQMREKSFGKTLASVTKEILGTAQSVGCRVNYKNPHDIIEAINAGEIEIPEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0H1</accession>
    <name>A0A1E5R0H1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Histone H2B.2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g4490</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the histone H2B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0H1"/>
    <dbReference type="FunCoup" id="A0A1E5R0H1">
        <property type="interactions" value="1183"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0H1"/>
    <dbReference type="InParanoid" id="A0A1E5R0H1"/>
    <dbReference type="OrthoDB" id="10254238at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000014">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="13">
        <location>
            <begin position="15"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ80394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="125" mass="13593" checksum="364FE98F5575A356" modified="2017-01-18" version="1">MAADKKPASKAPAKKTASSAEGAKKRSKVRKETFSSYIYKVLKQTHPDTGISQKSMSILNSFVNDIFERIANEASKLAAYNKRSTISAREIQTAVRLILPGELAKHAMNEGTRSVAKYSNSVSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0H9</accession>
    <name>A0A1E5R0H9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA replication complex GINS protein SLD5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g4445</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GINS4/SLD5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0H9"/>
    <dbReference type="FunCoup" id="A0A1E5R0H9">
        <property type="interactions" value="647"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0H9"/>
    <dbReference type="InParanoid" id="A0A1E5R0H9"/>
    <dbReference type="OrthoDB" id="338231at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11711">
        <property type="entry name" value="GINS_A_Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21692">
        <property type="entry name" value="GINS_B_Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008591">
        <property type="entry name" value="GINS_Sld5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031633">
        <property type="entry name" value="SLD5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038749">
        <property type="entry name" value="Sld5_GINS_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21206:SF0">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN SLD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21206">
        <property type="entry name" value="SLD5 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16922">
        <property type="entry name" value="SLD5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007764">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160059">
        <property type="entry name" value="PriA/YqbF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GINS subunit" evidence="7">
        <location>
            <begin position="167"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="DNA replication complex GINS protein SLD5 C-terminal" evidence="8">
        <location>
            <begin position="315"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="227"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="375" mass="42961" checksum="F7853E8413298BEA" modified="2017-01-18" version="1">MDIDDILADLDDTTNVYHPPSSSYGGRDQSINTSATVLPSDAGLLDDEQDMQTGLGKDKLRTNIRLQNQQSNVVQKSPAEDYQELVTIWRNERMSPELLPYPKNLMKRILAQIEAQVERIEFISMGYDEPTTTASENFSTNDHAGARAKKLQKLQSLESTDVFDKEDEENYDTEEYRQNRDKKLPLLCMEAELERVKFLVRSFIRCRLSKIDKYLVYIRQNDVPTEELDEDEHQGIFSERNDSSGNLSDSQDLESKRHPSKLVSSGLLSPTEYQYLIKHSDILLKYFNNTVLQHMPPSLQLINDTESTVNMVDEPDMEQFVFIFVRGSNDEGASNEFDVHINGLDEDVTLITGGIYVMRYNVIRSLLREGVVVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0J3</accession>
    <name>A0A1E5R0J3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pescadillo homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Nucleolar protein 7 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">NOP7</name>
        <name evidence="8" type="ORF">AWRI3579_g4631</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the NOP7 complex, composed of ERB1, NOP7 and YTM1. Within the NOP7 complex ERB1 appears to interact directly with NOP7 and YTM1. The NOP7 complex also associates with the 66S pre-ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the pescadillo family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0J3">
        <property type="interactions" value="1374"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0J3"/>
    <dbReference type="InParanoid" id="A0A1E5R0J3"/>
    <dbReference type="OrthoDB" id="10264910at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070545">
        <property type="term" value="C:PeBoW complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd17709">
        <property type="entry name" value="BRCT_pescadillo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000067">
        <property type="entry name" value="Pescadillo homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03028">
        <property type="entry name" value="Pescadillo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010613">
        <property type="entry name" value="PES"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12221">
        <property type="entry name" value="PESCADILLO - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12221:SF6">
        <property type="entry name" value="PESCADILLO HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06732">
        <property type="entry name" value="Pescadillo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="BRCT" evidence="7">
        <location>
            <begin position="348"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="449"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="567"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="465"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="506"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="522"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="577"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="596" mass="68907" checksum="FE8F5DA2D4D6E47E" modified="2017-01-18" version="1">MRIKKRNTSGNARNFITRTQAVRKLQLSLADFRRLCIFKGIYPREPRNKKKANKGSTAPTTFYYFRDIQYLMHEPILHKFREHKTFVKKLTKYLGKGQVSAASKLETEQRKLHYNLDHVIKERYPSFPEAIRDIDDALNMLFLFSNLPATDRVSAKITKEATKLTNEWLAFVSRERLIRKVFVSIKGVYYSANVRGEEVRWIVPYKFPENIPSDVDFRIMLTFLEFYSTLLHFVLYKLYHDAGLVYPPKLDLNKEKLIGGLSSYVLETQEESQKSGIDANNSEVGATVDNEALAKALAADKQTTDAIDEAEITEENELEENETGLDTFEDNNKNKGDVLEQPKMFDSPQAVLFNNFVFFIGREVPIDIVQFLILSCGGKVISEIEIDQLKTKPDLSQITHHIVDRPTLKNRVAGRTYIQPQWVFDSVNKGELVAANLYLPGETLPPHLSPWGDSLGYDPTAEETKDQDDEEXXEEEDEEVEEEEEVVVAEGDEDQEEDEVYDDEVLAQKELEMESKGVSYTEGKDQLPAEKRTNKKRKADTADEEKDLKLIMMTNKQRKLYKTMQYSNNQKEDKINQLKKKKKEIEKKKKQLNKMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0T4</accession>
    <name>A0A1E5R0T4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Carboxylic acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4601</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0T4"/>
    <dbReference type="FunCoup" id="A0A1E5R0T4">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R0T4"/>
    <dbReference type="OrthoDB" id="5296287at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015355">
        <property type="term" value="F:secondary active monocarboxylate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035879">
        <property type="term" value="P:plasma membrane lactate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="335"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="367"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="396"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="430"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="47"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="472"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="502"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="512"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="521" mass="57963" checksum="42E44AD16F4F2CE7" modified="2017-01-18" version="1">MNISSKIKNHVKTSLTTIIPTKETINKGLARYPINPFPALSKLSLLQWQLFFLGLWMWILDSASFFSVSLTTSYLAEKFDKSIVEINDGIMYSLFTRVAGAVIFGIYNDMFSRKWTYVVICSLLCAIQLGTSFVQTFNQFLACRCVFGLILGASFSVGNITAVENINASKNKNSKNDIAGLLSGVFQAGYPMGYLIAQGATRGLVESHPSQSWRILFWFTSALCVPFIVWRSILPESPVFSENQKLLLNQNNTGSKTKKFFLDFQKAFQNYWDKILYQCVLMGAFCYTSHGSQDLFPTLLKSYGFSASKKSTINCVANVGCIIGGIIGAHAANYLGIRFIILCGIVIGCGVIYPWGYVEQYKICSAFWLQFAVQFGFANVPKYLHELSSYGEMKAFATIIIGFSYQIGTLVSAPASTIQSKIAAENNNNYSATMSVFLAIVFVILFVIVLFGIENRDSDGFVLREGDVDEEDTTDAQYSMNSSVDEEKGLQLDKNTSFESQVKPETQYVEQAQERESSSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0W8</accession>
    <name>A0A1E5R0W8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UPF0662 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0W8"/>
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    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0W8"/>
    <dbReference type="InParanoid" id="A0A1E5R0W8"/>
    <dbReference type="OrthoDB" id="2011986at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018810">
        <property type="entry name" value="UPF0662"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28086:SF1">
        <property type="entry name" value="CU(2+) SUPPRESSING AND BLEOMYCIN SENSITIVE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28086">
        <property type="entry name" value="UPF0662 PROTEIN YPL260W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10303">
        <property type="entry name" value="DUF2408"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="67"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="222"/>
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    </feature>
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        <location>
            <begin position="597"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="67"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <begin position="204"/>
            <end position="215"/>
        </location>
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            <begin position="602"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="612"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="639"/>
            <end position="654"/>
        </location>
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        <location>
            <begin position="672"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="723"/>
            <end position="737"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="737" mass="83487" checksum="3404C2716058EEF2" modified="2017-01-18" version="1">MTYSGEVYKEEEPILEDFRNIRQELLQLKKDRRQYLNSKNIYKIYELVLTKVNELRRIRAGIDMSDTNKSEANVGGANTETDEESANKESVDSANTPFVQPPNRVDSVLDDIFQLLSLSFLTCGLKNTAPATYSSLSTVQRLLEHLEESKIYTFHDLRPIKARLEEISKIVENSNFVHFTSNTDSNKTTPQPTDSNKTTPQTTESNESNENSGNNQVKNEINNGENKSVADSFMNVHFTTTTLKQNDILEDSILKKKLRNCLIEYERIENNIEKIDPKLEPVMEELYGLRRELLSLTVASRTGSLQKVTGESGKNVNDSNKNFTTANGISRSSSNTSMINYGTPSYLNKYNDKLLSIENQLAQALGTAPGMALSVSTNNLSGQTVLNGLIDDCHDLLNDLTHNHYYTETDENYYNDPELNRIYNTLVQTKTNLENLLITRRWTLRETDLFQYQKKLEDIDRLRTNGKFFAGSSSLTSSVEEQKSQSILLYLLRRCYAIIYKLLESSEPVSESLQRVHNQLSTVRRCLLELKRMGGVDNERELYPYQMKLASLDNMKVDGKFYDEDGNVPEGQGTLCCLLNECFDILHELEIEAEDKEKLTSRESTPVNLTPSTTISRTNTNISTSSARNTQNNNNATTPLKNSTLLHKSSSLGNKPSGKEKHTNSSSSMQLTKDDSTRDVSELGKSNDEDEIAELTKNMQYKKFLNSQGHEDDGESTYSLDESLVEGEEDDDEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0Z6</accession>
    <name>A0A1E5R0Z6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g4598</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0Z6"/>
    <dbReference type="InParanoid" id="A0A1E5R0Z6"/>
    <dbReference type="OrthoDB" id="14911at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ80552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="325" mass="37144" checksum="96105D3C7709CBC5" modified="2017-01-18" version="1">MREHLGCDLELVQHVDEAFTQYREYSSKHYSELHTINKRFEGAESRESYINSSVIELAMRELFDLNCTAAWSNIHGDEAPLQHSHILETGANPYQYRKQLNYAFSPYKEQFIGYMXFSQDDDFYXVDLXKSLLYLQYGPFGKKTISLFSALTFSSLGSFVSSLYKLANVVDPFSLADPLDESFCEIWHATASKNTAIFRSVFYCQPDDLVRTQDESKLFAKYEEMLVDYQRELGANALLEVSFEPVDDFIASSAKDEATKNAVLRLSAVNTQNKVAARKRIANELLCGVIGHLVLLPCRNFEKGVKDKEEGSTLPMTSEIFFRFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R189</accession>
    <name>A0A1E5R189_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">amidase</fullName>
            <ecNumber evidence="3">3.5.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4262</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a monocarboxylic acid amide + H2O = a monocarboxylate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:12020"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:35757"/>
            <dbReference type="ChEBI" id="CHEBI:83628"/>
            <dbReference type="EC" id="3.5.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.4" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R189"/>
    <dbReference type="FunCoup" id="A0A1E5R189">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R189"/>
    <dbReference type="InParanoid" id="A0A1E5R189"/>
    <dbReference type="OrthoDB" id="6428749at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004040">
        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072:SF4">
        <property type="entry name" value="AMIDASE C550.07-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072">
        <property type="entry name" value="AMIDASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001221">
        <property type="entry name" value="Amidase_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="7">
        <location>
            <begin position="101"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="5">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="254"/>
            <end position="257"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="588" mass="66452" checksum="712EF497512895BF" modified="2017-01-18" version="1">MTFENENALYATEDPKSFEKWVPLIKKYTDDLTKQCKEYDSEFYSKVLATVAPETSLDSQAINALEYLPKLLSKEELAIVSEYDIATLLEKQKTKKLSALDITNAYIKAAIVSHISTNCIMQFLIPEALETAKKLDLYLKENNNELYGPFHGIPISLKEQINYKGKITTASYVQYLTNIPEQSGVTVQILEKMGAVFHCRTSQPQAIMHLDTWNNIIGRTRNPLSTKLSSGGSSGGESSCIASHASVLGIGSDIGGSIRAPAAFTNLYGLKPTTRRISLMNGLSGGKGQESIVACQGPLARSIEELDYLMESYINNGQPWNYDPLCVPVPWKDTSVDSLTKGGKKPLRIGVLFEDELVTPFPAITRGINHTIETLTKAFPGEFEFVDLKKHWYTEEEMKKIYNNNVTLYTVDGNKVQMSMFKESGEPILPLTRHFFEFGGGLEHSIYENKMLNYQRDTLKVEMHEKFFNGLNLDFILSPTYPAPAEKAANSLYWGYTSFWNMLDYPNVVFPTGISHDPELDVINEEMKFKSNNYEKMVWFDKSGKIRYDPMDYVGGPVALQLTAKRFEDERIVACIKKWNQALKVERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1C0</accession>
    <name>A0A1E5R1C0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Inhibitory regulator protein IRA2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4517</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1C0">
        <property type="interactions" value="174"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1C0"/>
    <dbReference type="InParanoid" id="A0A1E5R1C0"/>
    <dbReference type="OrthoDB" id="28245at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05392">
        <property type="entry name" value="RasGAP_Neurofibromin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023152">
        <property type="entry name" value="RasGAP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF142">
        <property type="entry name" value="NEUROFIBROMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00509">
        <property type="entry name" value="RAS_GTPASE_ACTIV_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GAP" evidence="4">
        <location>
            <begin position="2020"/>
            <end position="2209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="318"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="461"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="614"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="760"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="819"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="960"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="320"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="465"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="614"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="645"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="671"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="761"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="832"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="960"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="991"/>
            <end position="1000"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ80363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="3391" mass="385770" checksum="9BEF2E3407104082" modified="2017-01-18" version="1">MSNNSGEDIPSINHELDYSRHILRCLIKFLPNNPNFCNSYKNIEEEEYFKKCKISLLYYSINNNINVLIEDIIVFLEERLLKFEEFSTNYVSLTNERIMNYQDYDSNELDNDNASANTSNTANTHTTTFTINVPSTDTSPHGNERTLPRYRTCKKNTKYPIKSIGISNYILVFLRLLSDICEVNYAYYETPFYKKLSLSEKKQHFDKYFCYSLQKDEKFFKVLIFGIIQNQHFPPLNENDINKTSRHAPKKLDPRLFKKILKFLIPLKSTYKLETCIKKIINNDKTLLPIIKNELLDGSLTKMQQQAKLNAKIPSSSLFSPANQNQNQNPSSTXSSGNDKQQPFNDSEEEHWDKIIGIKELRDQIDTNIEYLLHYIAASNSKDYYHFLEQILVSPAQLEAHFPSSARVVGNNYTTVYRTGKASKFHEENXTKKNQNHNHHQQTQNDHDGATEDIRSIKSSDSFHTTTTNNNNNEYSAHSTSNKTSSTSEKKPFALPSSLNIYNSELLYATEYLSFVVFKSTSDMTNYIKLLIKLLGMAKKPIFKKCCLFAFTHCLELWTISKPVDYNKFTHENTTSDNVLLNKEITQLFNLVYSTFNASQLMSNAYGNNYINSPSTTYPGPTPPSTTTSSAVPSPSTPHPQQQQLYHTQTADPQHQPGFSSPVESHLPGHSASFSATTSPFATEAQRAGAPSFSGRVLSSMGRTMSPANTIATQSTYSSSGNSLYASQAIQNLHAASAVPAAQNTVPPASSLYTSLHFQATTPTSTPSSTSLDSAGAAQTAAGTTAEHTAGHKDXHLNSPTFNHNMSADVEHHHNYETQLDSRDSSNDTNYFTKSFTSPSTSSKDSFEAANIEEYSNNLMKYISKFLTIDEPTVTSKLAGTYTLGYCFLDSNLVDPGDVGILRALVTLSILHVDVFRSFLNKTSFKNFYDANDISNEPLDLSPVTTPLSEASLSSILINSPPPLTTSKSTPSPPIFSPAGTTEYKMPSLDTRVTMSSTNISKXSKRSRANSRLAPLIGLGSPSSSPPSTDAHPVSSTSAAPSVASSSSGSFLQLQTPIPTKHFSVGSQKLQQNLKKMTSSLLPSSYSTNKEVRFWSNLMKYLTISNTSSHQITDKSMILILNSVMFLYSTASIISIVSPSSPIVIFAKRLYFNLIKIFDYSPQSPLPENLTLLYSVLKRNPTIQTKFNIEFMTPSIYLQGDKFIDLIKTRFITISEKGVYKKLCAYNLYCMTEGMKFFFQVPFEFKSRAVYFTKMLRVIRELGFQLSTNLLQTIDFIDENASQAVDMFLQGQPTSTDERIDFYVGKKLVDTERFFKIDPTKTETFSVLDEKSFAAILSLTKKDLKGWMTNSKNKEHLVHYNAKPLSKYDTSVTSDITIAILCNIVNYFKTATSLFEEGVLTTKNQAQPQYLQKTLFTGLLCGNKKLVKETIACIETSFSLAHIDSSDERFATFYSMTGHSCLGLSIGAFNLYAPDSTRLLLLKTLVQFLEQRAVLNEQLYQKTKFQNLDEDDRKIFEQLHESLGRTSYLSLCLHDVKAHAIIRELFSMYQKEIYYYGKYIGKNLADMHITNTEFTAAMANCTTVYGPIAFQRHIRADILKLIHFPNKKLFDACTLIFEEWFQMGVIGSENLSSHEQNKFRNYSGLLAATSGIFNFRNSELLGKFPYLTYAKNLITKEIDYTIRRHCELLDSPDLLTRENAKDVLSLELNSVHFDNLFDNLLKKLECVFVQKDGQNVENFTLLEQIILVMRSLMERDDIKVIIVTTAKVLKIVSKILELVEKLPKESVNQYKAMIYTSKLFLSFKHSEKFMFLKGDHMLKNSWIRIVLNWFGNSVFKEMDYINLTKPHREMDLARRDIDYLYLDTSIDCAKALAYLTTRVVLEVPQAANEEDYKRSKSVLFGNYFSILLKALEKTADYESIPFVLRHKVLIFNENVIICLKNLLNANSDVGLNFALPMGYSKNRMIRLTFLKVFVDISKNVRMNSAELNASQEEKINEVLQRMVTHPELLCLIASCCPVNEVESLSTFLVSIFHSKSLSHILISYLIIDEIENSTRSMDILRRNSIATRTLAMFSRICGHEYLKKTIKPVLDSIIASDEDFEIEKSLPSQESAEINVERFGKYMVKLTDSICSSVNDIPPEFIYISQRIREAAKKKFPGSELVAVGSFIFLRFFCPALVSPDTENLIPSLLTGNRRSFILLAKVIQNIANQSVKSLKWPLIQDKMDLLETCSNRIMEYLANISDPNIKTDIPIHYDAPIGKQNNLGTFHKILYNHKQEMRNIMVKSVSSDQSLEDLLDTARIVDEALLSLGQPRVEFKDEIPSFIKENVDKYSVLYEFLSRYYLKQQNQPEEAQFAHEAIDREGNPMLVYSMKTIGESTLDNEQVIYKLILLASRFWSKQHIVLFDCTGFPSVNLEKMKNIFSLYLNLLPKNVFLNHTKLILYNVNDAFMTFFMSMERHIFKHICPVSPAHEFLNSDCSPDIVNSLSLTKYSTDVYNDVRVTLHNTSLYEQQKGRFVPVTLKIGNKTFQIVKESPKRMKLAGLEKVYEVYYNDVYEIRDILSAGTSSVTGIPFEFTLQLKTDNTLIMSTQKYLEVLKIFYYAQTKLDEESMNLGYQVSKESVISKSADSDLDILARALFVTFAGLSSCDDEIKSTSYALLSVIQQSFELDLGQRLHNLPEVFLPDDNDIFTQVLFENLARTKPELTYYIWNHFLEGINNVLDDSQIVHCINCLSPWTSNIYSHIYRADKVLGSEKCALIIRSLIKVSIRKTRFANAFKYFVWSDLILDSSLLGMITDEIVNHAIDRESEGSDWTSCISILTRISSVEITGQIIQRLLKLIKKFIPTLGSEQNINNWSEIFILISVLQDLSFNTSMNFEICLPEILYIVSMLIDLGPSKLRVSLHRLLVNVCSTFIINSSEGSENIKRLTEVIEPLSSQKTKVLFGFQQEAEFAHNYTNGTTLLSKLTSLNQLVNYMTCIIRYGSETASYSTQWQAKYNDLLIETIFGDSYFLSSRAMTIFSIISRDGISDELIYKLLTGTMQVLGEPNVTEEGMIYLISHMVSYANFIQGIKTNTSLMAQLYWFSFSYLYSPSVILFHAGLLTNLVAFEKVFDVLTDKQTVANYLWENNRHGLNETLISIEHLQDIECTQQNLIHLTIHFLAKGALVLHTKATAMSGFKTFFKIVQNNERLSQEKKHMGFLLFLYFSLPSAEFYDFCESVNLPSEKTKLDDLCEAPKVLVDWLLDRDDDYKKVVLYQASHYASSEHFEDSDKLKVFSIALYVAKHKPKLFFKYMSKTIFDARRIEKQGILLELLRKAFDISNFAIMHERYPNVARYEREVSEKMKHLKMSGIKTMTFGSTSPHVLAGVVEESDQVFERKKLSVTLVDEIINT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1C3</accession>
    <name>A0A1E5R1C3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pre-rRNA-processing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g4095</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Component of the RIX1 complex required for processing of ITS2 sequences from 35S pre-rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the RIX1 complex, composed of IPI1, RIX1/IPI2 and IPI3 in a 1:2:2 stoichiometry. The complex interacts (via RIX1) with MDN1 (via its hexameric AAA ATPase ring) and the pre-60S ribosome particles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the IPI1/TEX10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1C3">
        <property type="interactions" value="262"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1C3"/>
    <dbReference type="InParanoid" id="A0A1E5R1C3"/>
    <dbReference type="OrthoDB" id="361362at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120330">
        <property type="term" value="C:rixosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024679">
        <property type="entry name" value="Ipi1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16056">
        <property type="entry name" value="REGULATOR OF MICROTUBULE DYNAMICS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16056:SF2">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12333">
        <property type="entry name" value="Ipi1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Pre-rRNA-processing protein Ipi1 N-terminal" evidence="8">
        <location>
            <begin position="158"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="406" mass="45995" checksum="3E4C31A4E3B167B2" modified="2017-01-18" version="1">MAKKKTLRQQDFQKKKLKVGKGKQQPTNITDTSFVAKQIRLPTQRPIKDELVNEYAKTVAKNAAEHAKAMGKPVTGPAAISNNILANKISLLKHHSPNMRKEALLFLSKNISNKALTENQMNIVVNNIYQIIIDDDVQVRNQLIDLLDNVGSVNSMVLELNLNKLVLYLCNSMTHIIPQIQRDSGKFLKILVKYCHDSIVRRFWCKILVNISRVLGWSLFVDVNKSNKITTSLQNITSNSNSLSSSVVSSNKSIKSKINNLEALNFFIKSGCQELFDQKKKMEGKNDXFDNNEFYLESNMKKYLISEVPQPYQYLKLFETKFEDNDVFSVSASNDTKLSGAQNFKDLEKIACEDYVIRCKLLNENFMKPFLFNLAPIVKDGGESGKVANTLLKYLETLQTELNAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1I0</accession>
    <name>A0A1E5R1I0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NGG1-interacting factor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4338</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP cyclohydrolase I type 2/NIF3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1I0"/>
    <dbReference type="FunCoup" id="A0A1E5R1I0">
        <property type="interactions" value="663"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1I0"/>
    <dbReference type="InParanoid" id="A0A1E5R1I0"/>
    <dbReference type="OrthoDB" id="3345469at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1390.30:FF:000001">
        <property type="entry name" value="GTP cyclohydrolase 1 type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1390.30">
        <property type="entry name" value="NIF3 (NGG1p interacting factor 3)-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002678">
        <property type="entry name" value="DUF34/NIF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036069">
        <property type="entry name" value="DUF34/NIF3_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00486">
        <property type="entry name" value="YbgI_SA1388"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799">
        <property type="entry name" value="NGG1 INTERACTING FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799:SF13">
        <property type="entry name" value="NIF3-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01784">
        <property type="entry name" value="DUF34_NIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102705">
        <property type="entry name" value="NIF3 (NGG1p interacting factor 3)-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR602678-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="297" mass="33226" checksum="F24172B3B8618B7B" modified="2017-01-18" version="1">MNLALNKTQTSKIVTVIQKLFPLKYADSKWDNTGLLIDCSSLSNKEFPEIKVLLTIDLTQKVCQEAIDNECNFVLAYHPFIFPNWKQIDPTKNPQHLSALKLIHHGISVYSPHTSVDAVKNGVNDWLSNTLVGQNIGSAQQVASSESKVIEAIEEDVGYGRYLDLSKHNLDLQTVIGNVKKSLKVSHIQVSIPDVLSTTKRNEIKINNVALCAGSGSGVFRGLTSQQWADVDLLYTGELSHHEILKYKEMGKIVILCNHSNTERQYLEQVMIHELSKVDSSIKYHISAFDKDPLETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1Y5</accession>
    <name>A0A1E5R1Y5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphoglycerate mutase</fullName>
            <ecNumber evidence="9">5.4.2.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3894</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.11" evidence="9"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1Y5"/>
    <dbReference type="FunCoup" id="A0A1E5R1Y5">
        <property type="interactions" value="1246"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1Y5"/>
    <dbReference type="InParanoid" id="A0A1E5R1Y5"/>
    <dbReference type="OrthoDB" id="4818801at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00186"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004619">
        <property type="term" value="F:phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000012">
        <property type="entry name" value="Phosphoglycerate mutase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01039">
        <property type="entry name" value="PGAM_GpmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005952">
        <property type="entry name" value="Phosphogly_mut1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01258">
        <property type="entry name" value="pgm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010713">
        <property type="entry name" value="PRK14115.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010718">
        <property type="entry name" value="PRK14120.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11931">
        <property type="entry name" value="PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="6">
        <location>
            <position position="9"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="6">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="8"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="21"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="87"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="114"/>
            <end position="115"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="183"/>
            <end position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="8">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="248" mass="27820" checksum="1F942FD254C96755" modified="2017-01-18" version="1">MPKLILVRHGQSEWNEKNLFTGWVDVSLSKIGEAEAKKAGQLLHEQKVKPDVLFTSKLSRAINTANIALAQTDRLWIPVKRSWRLNERHYGSLQGLDKAETLKTYGPEKFQTWRRSFDVPPPPIEATSKYSQKGDERYSDIPNDLVPETESLSLVIDRLLPYWQDEIAKELLEGKDVMIVAHGNSLRGLVKYLDNISDADIAGLNIPTAIPLVFELEPKTLKPTKPYYYLDPEAAAAGAAAVANQGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R256</accession>
    <name>A0A1E5R256_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Nucleolar protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g4189</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R256"/>
    <dbReference type="InParanoid" id="A0A1E5R256"/>
    <dbReference type="OrthoDB" id="1099063at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF62">
        <property type="entry name" value="SERINE_ARGININE (SR)-TYPE SHUTTLING MRNA BINDING PROTEIN NPL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="60"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="135"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="207"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="295"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="312"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="321" mass="35466" checksum="68BF3081D0EC9918" modified="2017-01-18" version="1">MSYEQEDIPVQDYETTNNTVEQPQADLGAPASDXAPVSAPVSGYIPPSAYHQAEERISETRLFVRPFPEDVQEAELVDIFQTFGAIKEIKILRGFAFVEFENKDDASRAMTETHGKLFADQPLDITYAKLQPIRYRITLKNLPESCNWQDLKDLARENGLESTFSSVNQRDFQGEGNLEFPTEEVLAEALTKLNNIEFKGNVITVERDDNPPPIRRSRGGFRGGSRGGFRGGSRGGFRGGFRDGGFRGGRGGFREGGFRGGRGGFRDGGFRGGSRGGFREGGFRGGRGGFREGGFRGGRGGYQREGGFRGGYQRERSPSRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R258</accession>
    <name>A0A1E5R258_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Endoplasmic reticulum transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4158</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BCAP29/BCAP31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R258"/>
    <dbReference type="FunCoup" id="A0A1E5R258">
        <property type="interactions" value="145"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R258"/>
    <dbReference type="InParanoid" id="A0A1E5R258"/>
    <dbReference type="OrthoDB" id="3972976at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070973">
        <property type="term" value="P:protein localization to endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008417">
        <property type="entry name" value="BAP29/BAP31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040463">
        <property type="entry name" value="BAP29/BAP31_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701">
        <property type="entry name" value="BCR-ASSOCIATED PROTEIN, BAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701:SF19">
        <property type="entry name" value="ENDOPLASMIC RETICULUM TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05529">
        <property type="entry name" value="Bap31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="95"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="BAP29/BAP31 transmembrane" evidence="6">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="194" mass="22028" checksum="A6FD5B623C9667D7" modified="2017-01-18" version="1">MSLYLTLLFLLLVTEMAILFVLLMPLPHMVRKRIGYMYNNLKASSQMKTVLVVFSILVSSLFADSMKRGARPLPLDRNLVTPDMLATKAYHQRNIYISGFILYFGLCIPIVMGVIAKLVKYEDTLKIQSGVAERTAENDKTENLRVDKTLLAELKEKRASLLALQKQLDNKNAFIDKQLDKENGTKTASEKKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2E1</accession>
    <name>A0A1E5R2E1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4257</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 81 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2E1">
        <property type="interactions" value="208"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2E1"/>
    <dbReference type="InParanoid" id="A0A1E5R2E1"/>
    <dbReference type="OrthoDB" id="4473401at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052861">
        <property type="term" value="F:endo-1,3(4)-beta-glucanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1170">
        <property type="entry name" value="glycoside hydrolase family 81 endo-[beta] glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005200">
        <property type="entry name" value="Endo-beta-glucanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040720">
        <property type="entry name" value="GH81_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040451">
        <property type="entry name" value="GH81_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983">
        <property type="entry name" value="ENDO-1,3(4)-BETA-GLUCANASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983:SF20">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-D-GLUCOSIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17652">
        <property type="entry name" value="Glyco_hydro81C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03639">
        <property type="entry name" value="Glyco_hydro_81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52008">
        <property type="entry name" value="GH81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184571" description="glucan endo-1,3-beta-D-glucosidase" evidence="10">
        <location>
            <begin position="21"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 81 N-terminal" evidence="11">
        <location>
            <begin position="430"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 81 C-terminal" evidence="12">
        <location>
            <begin position="755"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="62"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="159"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="318"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="323"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="347"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ81062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1121" mass="118982" checksum="137AB6260A73E62B" modified="2017-01-18" version="1">MVCLKQLITTVPLLVSLAKGAAIISENGVGTTGTLYDEYDVTETTFGATYTAGEPAVILSSDESDSGTVATTAASSASPSASSSGFSESSVSASVNSNSPXYTTLSSFEXKTVTSASVLPNSQTSSTTYLTTILSKSTSTLAPSVNTNSNFNDRVSASLQSKSGSSSESVTGTVSRVSSATTTTSKSTFSDPGKVYISSLVSPSDVTETSIVFTATSSVAPVSSLTGSIISASVATTQTQNPLVTQSFSTSSQSVSINTGSLSKDVTVTTSTPTGSAATSATVVVTAPTVVSTSTYQTVTVSESASTFYSVSSVTPKSSAHYTNETSTASQTSNTQAASTKGSAIPTTTSTTSSGVSLSTTTSVTKSLVTLSLMTTSAVTSTLTSTTVVASAVTTVDLFEPIATNAPPSSLGSMSNDWSFDSNVETNGASDGQTNKFYINLLLDNQEYPAFAYPYILWKLTSTFFGFAVQHTTVNDYTFSQDMDNGNPRVFTNPLNQADLVFSASTFNSENDLSLTVSDLQQGSVLATLQSSSNGGSNYIDIPIVHGMGFSTAIYHGGLIPRLDSAMNIQSVLSESSSNLASGISKYRITINNVDWLVYVTNPSSSVDFEFTTAYGAGTGYSVVGSTAIDGLIIQACVAPSSTDLEYYYDQAAGMYVTSYEMNGTSDGISATYSYDYTTQGASASGNTIIFVLPHQIGVLDSNTANTNTGIEIAALVKGVATGFLTNTIGCIETLNTEISWLPWTSQLGDTKLQYTSSQVQKIASVANSELNVNIKDAIVGLNSYYLGKVLDKYAYILLTVSEIVQDSSVTASTLESMKEAFDAIFQGQQAFPLIYDSKFGGVVSNCDWSSMSSTSDDFGNSHYNDHHFHYGYIIHAAAVVGYVDQQAGGTWAKDNEDFINALIRDVANPSSSDSYFPVSRYFDWFDGHSWASGMYYDINGNDEESTSEDYNFLYAMKMWGTVIGDKSMEYRADLMIKAMSRSLDAYFYFTDENTVQPSEIIPNRVAGITFENFLDYATYFGLNTEYIHGIHMLPITPASSQMRSATFVQQEWESLLASIVDDLSDGWKSILTLNQALYDPKSAYDFFSSSSFSSSYLDNGQSQTWSLAFSAGIANSLGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2J2</accession>
    <name>A0A1E5R2J2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3852</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R2J2"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="306"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="348"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="155"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="309"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="326"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="348"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ81126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="399" mass="45542" checksum="1C776896251E6F17" modified="2017-01-18" version="1">MPSSKGQKQNACAKPRISRHEHLSDDSQDTRVDLDSAVSGNFLMSALLCQQDETRQSETHCKVXETLSDQLNEIQCDLYNQKAGTRTLVDEMLQEINSIKTTSKKISNSFNTALIHTVESAVHDYMCKQTSTLTQTISELDKSMQSFIKQMPSTMQKQITQFKKLETELHDQEKTLADLKSLTGLEKDTNAIDYLKVELHNLKKTILAKIDMVQSVQTDFINFQHKSSARFDGISKLLDEKQTQISMLESKIEDQCHTIDKLENKSLLKDEHLQHFEKKFKSQDAKIKILQQNINKLITVVKSNRKLKQXSQSXXNSTKNQQNESSKNDETVPTKELIIRESKFTAYSNKKQLPQPXQKNSKKNDCSRIVLKYKVDKQKKTMSPSTKAAKPAGLFDIPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R350</accession>
    <name>A0A1E5R350_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein ECM13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R350"/>
    <dbReference type="STRING" id="56408.A0A1E5R350"/>
    <dbReference type="InParanoid" id="A0A1E5R350"/>
    <dbReference type="OrthoDB" id="5431245at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="32028" checksum="C47F6340576C7883" modified="2017-01-18" version="1">MYQSEELSLTEKYLLANKVRTKLFSCVQQNNSASSKSDNVTKSKTLNKKHGNAVNRHRSHGQVDFDLRVLVGHAMVLDRLMDNINQQVAYNEERYVERENTPVPAVSVPSSLRNEEKYEYYYSDSDSDSDNDSTEEEDNDEEGEEEEGEEKEFQYTTAPVHTLYSQSNKNILLMDPHRTVKHEYEQDQQIDILEEQCSSDEDSSASEEEGQDEEGKEDENHQYEVHHQDLFFLSQRAASNGSPSDYTVSHSGHGIYTNTNHISNHSTEEVDLGTMSPICV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4G5</accession>
    <name>A0A1E5R4G5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">40S ribosomal protein S23</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3646</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4G5"/>
    <dbReference type="FunCoup" id="A0A1E5R4G5">
        <property type="interactions" value="838"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4G5"/>
    <dbReference type="InParanoid" id="A0A1E5R4G5"/>
    <dbReference type="OrthoDB" id="1713912at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03367">
        <property type="entry name" value="Ribosomal_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000007">
        <property type="entry name" value="40S ribosomal protein S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_uS12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005680">
        <property type="entry name" value="Ribosomal_uS12_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003254">
        <property type="entry name" value="PRK04211.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00982">
        <property type="entry name" value="uS12_E_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002133">
        <property type="entry name" value="Ribosomal_S12/S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ81809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="145" mass="16038" checksum="F36A18A50C69FCEA" modified="2017-01-18" version="1">MGNGKPRGLNSARKLRVHRRNNRWAEQNYKKRLLGTAFKSSPFGGSSHAKGIVLEKIGIESKQPNSAIRKCVRVQLIKNGKKVTAFVPNDGCLNFVDENDEVLLAGFGRKGKAKGDIPGVRFKVVKVSGVSLLALWKEKKEKPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4Q5</accession>
    <name>A0A1E5R4Q5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3602</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4Q5"/>
    <dbReference type="InParanoid" id="A0A1E5R4Q5"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006694">
        <property type="term" value="P:steroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002225">
        <property type="entry name" value="3Beta_OHSteriod_DH/Estase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF844">
        <property type="entry name" value="NADPH-DEPENDENT METHYLGLYOXAL REDUCTASE GRE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01073">
        <property type="entry name" value="3Beta_HSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-beta hydroxysteroid dehydrogenase/isomerase" evidence="3">
        <location>
            <begin position="13"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ81897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="349" mass="38844" checksum="9F84BC5F5D8949FE" modified="2017-01-18" version="1">MNQNKKYTPTVFVSGATGYIAQHIIKQLLDSQAYRVVGSVRSMDKAARLHELFNNNTNLSFAVVDDLCKVDAFDQVFQDRGMEFDYIMHTATPVDLFCNDLQKSIILPAINGTKSIFQATVNYAPNVKKFVYTSSYGAMRSPQDNLDSNLTFDETSWNKLTLEEGLQNGKNAXHYAKANAEKLVWEWEKTLNAKFAVVTVSPTFTFGPQCFDASVGKTLNASCECINLLVHSKFGEPVNNAIKGNFADVRNVARAHVECITNARLNGNRLIINQGKFCLQLIANIVNDKFPQLRGKIAVGCPDTVDQVCRSFAHLNDKATKELLSFEFFSLSQSVTDTVAQILRVEEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4S3</accession>
    <name>A0A1E5R4S3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphatidylinositol 4,5-bisphosphate 5-phosphatase INP54</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3810</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4S3">
        <property type="interactions" value="96"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4S3"/>
    <dbReference type="InParanoid" id="A0A1E5R4S3"/>
    <dbReference type="OrthoDB" id="62798at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF275">
        <property type="entry name" value="LD06095P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="367" mass="42075" checksum="E7D3E31AEF71BB09" modified="2017-01-18" version="1">MRLYVSTLNCGKNTLGKKDHNTITQLLANTDIADTPYDLYALGFQEYVPLPSLGDGKYFNKHLQSXSKSIVLYLNKLHENVSYELVKYNFNGGLSLLVFAKTSLGENIECHLLNSRCGKFGSGLKGASLIRVQHTGPQFWAINFVTSHLAANEGFYNKRVENIKYIMDDFNNQLGNPDFFNINNEHVLFFGDLNFRNTSLDAIDITENNCFIDQKGFERDELYRYLHDSNYFQFNEFPVKFKPTFKYKEQPHQDTQPRYVYNLKRIPSWCDRILLSKALFDSSIDHCYNSVPRLTPFLRATDHQAVTLSLTYKPAFQSAAPPPALKHHLTPDSSLIDFCLKYAFTVYENKTIILLAVAIVAALLLSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4W3</accession>
    <name>A0A1E5R4W3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA pseudouridine(55) synthase</fullName>
            <ecNumber evidence="3">5.4.99.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3778</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a uridine in mRNA = a pseudouridine in mRNA</text>
            <dbReference type="Rhea" id="RHEA:56644"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:14659"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pseudouridine synthase TruB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4W3">
        <property type="interactions" value="338"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4W3"/>
    <dbReference type="InParanoid" id="A0A1E5R4W3"/>
    <dbReference type="OrthoDB" id="9995526at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160148">
        <property type="term" value="F:tRNA pseudouridine(55) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01080">
        <property type="entry name" value="TruB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014780">
        <property type="entry name" value="tRNA_psdUridine_synth_TruB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13767:SF2">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE TRUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13767">
        <property type="entry name" value="TRNA-PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase II N-terminal" evidence="7">
        <location>
            <begin position="65"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="377"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="387"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ81908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="417" mass="46785" checksum="DFAD2506A2880261" modified="2017-01-18" version="1">MNGIFAVEKPSGITSSQFLSKLQRVLTSSEVFQKEIQAARNVRIEQYKRENKGKHPPNRILNRVSKVKMGHGGTLDPLASGVLVVGIGQGTKELANYLKGTIKCYEAEALLGISTTSGDCEPNGEIISVNKTQHLQNLGEEAKKELEEDLVQKFKGHLKQTPPIFAALKMNGKPLHEYAREGLPLPKPIEPRQVQLYDLEFVKDDFLTSNHNYTFLQITDDTVKDLSKLSNNPTTTQLYYADEYCTAKGLPLGEPIQQGTLQKNFICDPSDEELVKMKDPGYKAPLFHFIAKVSSGTYIRSLIDDMGKALGSSAYMVSLKRLSQQNWDLNKGNVFKIEDFENKPERVWSTVLKKVLDQGASIDIQKELQQEQDACTTAKKEQKLDSDQADDAEKIEDENNKDEQQXDIEPPTKKLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4W8</accession>
    <name>A0A1E5R4W8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Bax inhibitor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3760</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4W8"/>
    <dbReference type="FunCoup" id="A0A1E5R4W8">
        <property type="interactions" value="606"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4W8"/>
    <dbReference type="InParanoid" id="A0A1E5R4W8"/>
    <dbReference type="OrthoDB" id="7933078at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291">
        <property type="entry name" value="BAX INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291:SF50">
        <property type="entry name" value="PROTEIN LIFEGUARD 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="241"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ81934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="300" mass="34784" checksum="342380C0C2C0108D" modified="2017-01-18" version="1">MGNPKDNATVPPYSGPQQTTVAAAEEENLLFTPDDFKYDQMVSSCDLSIRYAFVRKVYTLLGLQLFFTFIAQVLFFYDIIPRYIIFKPIFQIPAFVGSFVLCIVLFMATTNDDDYAGDEETGESRRPFFAPVIRMQYKTQLFLLGLFTVCESVMISYITVLYNPRIIMSAVLCTLVIVASTTLFVFRQAKKYEKNNGSLEFEEAENRSLFKIYNVLWILTTFLLGFGVIMIFMPYNSTRELIFSWIGAILFTLYLYVDSYMVLRKVRPNEEIRCCMMLYLDIINLFLNILRILAHNNNDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R534</accession>
    <name>A0A1E5R534_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">WD repeat-containing protein JIP5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3519</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R534">
        <property type="interactions" value="125"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R534"/>
    <dbReference type="InParanoid" id="A0A1E5R534"/>
    <dbReference type="OrthoDB" id="2288928at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051179">
        <property type="entry name" value="WD_repeat_multifunction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857:SF8">
        <property type="entry name" value="ANGIO-ASSOCIATED MIGRATORY CELL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857">
        <property type="entry name" value="MITOCHONDRIAL DIVISION PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="417"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="417"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="445"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="485"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="524"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ82025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="580" mass="64807" checksum="BEE9D6A149BD0226" modified="2017-01-18" version="1">MAKKSKKKSNSASLEQLESNCSPILEIKSTDENYFFNVVPHPSKPLLSIGLATGHVFTLQYDAQKLQDHVNVQRKASAKSEKENKNQTEQSSSTTEKSKVSKNKIFWKKFNLKDLAEGNFDQEFIKILWKTKRHKGSCRCLVFDPKDDGETMYSIGTDNILKKADTMSGKVIKKIDLNSVINAESETKTVKITKLVKSPTHNFLLLGSESGNVIVLDSNTLKVTNNELKQIHMGDAINDMFQYVKKSVYKYISLGQTTMASWDSRFVEKNNLSNDSTQSNKSVHLSDDQEDEILCGTFVNPDEDDTIVCGMGEGILTLWKSSKNDLQDQLSRIKIAKGESIDCVVPTLQDDNCVWCGCSNGNIYKVNTKMGKIVEIRKHSKFDEVTFLDLDFEYRVVSXGMDSLKVWEFLEDGDDQIDVSSEDSASDSENDSFSDSSDNDDDLLSDSASXSASDSASDSASDSASDSESESLKSDDSQIWEELNNKNSKTQPASKPQDVKAELLAELESSSDEEGSEQQKSIETVXNTTXDNNNINKKHKLDSPNIQPLPNKKNKKQLLKAKQLKNIQNHEHGIRKFEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R548</accession>
    <name>A0A1E5R548_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sm protein F</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3506</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the snRNP Sm proteins family. SmF/LSm6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R548"/>
    <dbReference type="FunCoup" id="A0A1E5R548">
        <property type="interactions" value="737"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R548"/>
    <dbReference type="InParanoid" id="A0A1E5R548"/>
    <dbReference type="OrthoDB" id="409625at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034715">
        <property type="term" value="C:pICln-Sm protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01722">
        <property type="entry name" value="Sm_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016487">
        <property type="entry name" value="Lsm6/sSmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034100">
        <property type="entry name" value="Sm_F"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021:SF0">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F SNRNP-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006609">
        <property type="entry name" value="snRNP_SmF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 10" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10 12" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 10" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="11">
        <location>
            <begin position="9"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR006609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ82019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="81" mass="9232" checksum="276AE88F734B6DA5" modified="2017-01-18" version="1">MSTAVQPINPKPFLMSMANKPVEIRLKFNKTVYKGILVSTDNYFNIRLSKAEEFIDGKPKGLVGDIFIRCNNVLWISEGRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5G4</accession>
    <name>A0A1E5R5G4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Small nuclear ribonucleoprotein Sm D1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3745</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5G4">
        <property type="interactions" value="1097"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5G4"/>
    <dbReference type="InParanoid" id="A0A1E5R5G4"/>
    <dbReference type="OrthoDB" id="9626941at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097525">
        <property type="term" value="C:spliceosomal snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027141">
        <property type="entry name" value="LSm4/Sm_D1/D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23338">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="5">
        <location>
            <begin position="2"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="135"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="135"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ82144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="169" mass="18647" checksum="6A3E933192E1D012" modified="2017-01-18" version="1">MKLVGFLAKLRNEQVCVELKNGSTVWGTLQNVSPAMNVILTEVTLSLPEKSSLGESFGSGAGSLYLVNNAAISGVYMNMHKDQKNNLTGNPHSTTLQYINIRGNTIRQIILPESLNLDALLKEESDIDDLNKLRKRGKVVNSANKKRKITQERIDDATPSSVGNKRXKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7I3</accession>
    <name>A0A1E5R7I3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">54S ribosomal protein L7, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3212</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7I3"/>
    <dbReference type="FunCoup" id="A0A1E5R7I3">
        <property type="interactions" value="651"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7I3"/>
    <dbReference type="InParanoid" id="A0A1E5R7I3"/>
    <dbReference type="OrthoDB" id="539541at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_uL5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_uL5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_uL5_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL5 N-terminal" evidence="4">
        <location>
            <begin position="128"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL5 C-terminal" evidence="5">
        <location>
            <begin position="186"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ82869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="288" mass="32248" checksum="F1FA8DE333348A01" modified="2017-01-18" version="1">MSLKRHFSTSSPAYKVGCSIVEPVHHLVKIDKRRLSPRFPELKYEKNDIRSSGFKPTVTHQDRVQEHYYHTVQPDLLLMAYKHGATTVKGAKARRWDGSSPYHVNRKAGKPAGAYSETADQKPITPKNIPQLSGLTVSSFVKEAKDNRLLQISSMLQLQQITGVKPKRVLAKTNVVSWKLKAGKVVGAKVHCKGRPASQFLATLSEIVLPRVREYNGIYESNGDKNGNITIGMTPEDVENFPEIEANQDLWPHLFGMHVNINTTARNDDHAKMLISGFGLPFTRGKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7J6</accession>
    <name>A0A1E5R7J6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 11">Protoheme IX farnesyltransferase, mitochondrial</fullName>
            <ecNumber evidence="11">2.5.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Heme O synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g3455</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Converts protoheme IX and farnesyl diphosphate to heme O.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the ubiA prenyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="11"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7J6"/>
    <dbReference type="FunCoup" id="A0A1E5R7J6">
        <property type="interactions" value="807"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7J6"/>
    <dbReference type="InParanoid" id="A0A1E5R7J6"/>
    <dbReference type="OrthoDB" id="5211at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008495">
        <property type="term" value="F:protoheme IX farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006784">
        <property type="term" value="P:heme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13957">
        <property type="entry name" value="PT_UbiA_Cox10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.140:FF:000004">
        <property type="entry name" value="Protoheme IX farnesyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006369">
        <property type="entry name" value="Protohaem_IX_farnesylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016315">
        <property type="entry name" value="Protohaem_IX_farnesylTrfase_mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030470">
        <property type="entry name" value="UbiA_prenylTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01473">
        <property type="entry name" value="cyoE_ctaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448">
        <property type="entry name" value="PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448:SF2">
        <property type="entry name" value="PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001773">
        <property type="entry name" value="COX10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00943">
        <property type="entry name" value="UBIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="9 11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="251"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="274"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="371"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="394"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="447"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR001773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ82882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="487" mass="53816" checksum="2AD622C6D695810E" modified="2017-01-18" version="1">MIPPTTSNKCFKGLVSTWKTLIPSGTITGSNVLSLSISRKSLLPSHMHRLASSSSQNNQNSNNKNSNRSKVAFNTSPVEFSSNISLDNRFSNQKIVKPNPGDTDFTLSSVAKKMTCTDAVTAESKSSSSSGNSNIPFKVSYVQKSTPTGSQKPLALDDVNTQKDLKKLCNAYLQLTKPRLTVLVMLSAICSYALSPFAASVPQLLALTIGTTLCSSSANAINMGREPEFDRRMVRTQARPVVRGLVSSNQAYYFAALAGVLGTSILYAGVNPTVALLGFSNIALYAWIYTSLKRKHIVNTWVGALVGAIPPLMGWAAASPLTLPGAWCLAGLLYAWQFPHFNTLSHNIKNEYKNAGYVMTAWKNPRLNARVSLRYSLLMLPLCFGLSYFNVTDWYYQIDSGIANLWLSYWAFKFWWQQRINYSTKVLKNSNQFNEGIKKANIFARKTFWISVLQLPAVLILAILHKKGRWDGLFGLDENTAPTKKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7T8</accession>
    <name>A0A1E5R7T8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glucosidase 2 subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3189</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7T8">
        <property type="interactions" value="558"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7T8"/>
    <dbReference type="InParanoid" id="A0A1E5R7T8"/>
    <dbReference type="OrthoDB" id="3973298at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017177">
        <property type="term" value="C:glucosidase II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039794">
        <property type="entry name" value="Gtb1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036607">
        <property type="entry name" value="PRKCSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028146">
        <property type="entry name" value="PRKCSH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12630:SF1">
        <property type="entry name" value="GLUCOSIDASE 2 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12630">
        <property type="entry name" value="N-LINKED OLIGOSACCHARIDE PROCESSING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12999">
        <property type="entry name" value="PRKCSH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13015">
        <property type="entry name" value="PRKCSH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184663" description="Glucosidase 2 subunit beta" evidence="5">
        <location>
            <begin position="24"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="6">
        <location>
            <begin position="549"/>
            <end position="696"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ82941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="698" mass="79986" checksum="C79B976DB19A350D" modified="2017-01-18" version="1">MVYLSKTTSLXVLLAVXLASCIAETETQLTAAPLRGVSPERYGELYQPLEIDGEQKWRCLNDPEILLSYDQINDNYCDCPDGSDEPGTSACSDTRSKNEESVRFFCENKGFKSYYIDSSKVDDGICDCCDCSDELRLSADTTNNNCYSMNQAFEKMVSEEKEKNQRGKAAFQNMLLSTELYQNKEKFYTLKQEIANLDEQVNSLSLDLENLKIKFNGSDGKKKLDPVAENFMNLDIHNNVVVPLEEYFASVGQVSLCYNTLDDLIKELSQNYNLYLNDPVVNENMDKYXELRDNLAQKENGLTVSANFEEXQLGQLINFYDEELYQLFVQRDFNLENLGSYKELLGKFSIAQAILGIKNHVYQTMINVIEGDMLPIMEDIAANYQQNLKDGAVQKAXTEFENFKKMYPTIPHXDKTLKISAQYKKSIELLQSIILEHASKMYQHHXRSESLGNENILDKLFYNNLMDNSLVEFAKDSSLIKNLMANVHIYKNKKLQAKYETTNTNLAEMRNLKSKMLEQLDYYEQFVEQNETETEKILAVLGKLDQEKSSCVPGIINKYEYRVCLGKPFQNGRRTKKHSIHQTGENTFTLGSLVNFEFDKQDTAXKYLANLRKAHLGDILSRYFMSYSAKDEFSQLSNGNNGLILNYKHGDKCWKGPKRSAKAYFYCAEQFKVLRVFEASKCTYEVELQGPLGCSLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7U2</accession>
    <name>A0A1E5R7U2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SPS-sensor component SSY1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3259</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7U2"/>
    <dbReference type="STRING" id="56408.A0A1E5R7U2"/>
    <dbReference type="InParanoid" id="A0A1E5R7U2"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF46">
        <property type="entry name" value="SPS-SENSOR COMPONENT SSY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="307"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="338"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="388"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="422"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="452"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="571"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="647"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="697"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="746"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="783"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="816"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="6">
        <location>
            <begin position="307"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="7">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ82982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="881" mass="98845" checksum="FD44B90A822773D6" modified="2017-01-18" version="1">MNTNSEKFDLSKKKKKTIFNQAAPGLFPPFSPGLSTANESQNGAGTSFCDTGCIEEGSIEMSLLHRNATQPNLNENIISKVLLHRNLENLPPSYRQDMVNDKFYLTNLLTDKPVRFDDFKSYEEALTKEDFLRKELKKHENHCAPSTAINNAEKFPEYGQQLNVVHDFEHVFSNMTASSQKREKVGVFRNIYTKLFSSSQTGKHPQGLFSISSKVRKNLPSASQSSSHQLIDQTKSQPLTQVIGDPEFGFIETRMEKPKEDYIITELYNPSDYITELQENVPGKQRHGRGGAFSTKNTLHRSLGMRHLQMISLGATIGVGIFLNCGRTFSISGPLGAFLGFFIAACMLIGTLLCFAEIIAVIPLNVGISGVISRFISDAFGFSVSWCYFFSYVVAFPAELLASVLLLSYYDSFENLINNNSKLAGVFIALTVIIIFLNLLDVRVYAEIEYIASIFKVLVISAMVITMILINCGVRAEKGDKNRYIGFKYWDRHKSPKKNITYGPFRPTFDLSDLGTGALKGIGGFGGVILSIIASVNVSAYAYIGSEIGFIAAAESKITFRRSIPSVTKRIFIRVILFYLLSIFVLGLNIYAGDPRLLRYSATQPSFNDIYTNYKKIVDELGGMQCSNAFQSNQALYFAENANQSPWIIALTTFNFCTLSSIFNALFVAIGLFAASSQLYAGSRILYSLALQEKAPVLFATCNSFGVPYLSVLFCGGFGFLSLLALHLETSNAFDNLVTIGTQGSILMWFAMNLAFYRFYHALKLRSDVVSRDSKEYPYRSPFQPYLSIMGMILTGLLLLFSGFQNCIQWNTVSFITSYCCLLVFFILFLGFAIFRYANVWKLEEIDLDTGRRELDREIWVEDRDYLPTFRELLVKILSYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R820</accession>
    <name>A0A1E5R820_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">[acyl-carrier-protein] S-malonyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3389</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>holo-[ACP] + malonyl-CoA = malonyl-[ACP] + CoA</text>
            <dbReference type="Rhea" id="RHEA:41792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="EC" id="2.3.1.39"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.39" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R820">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R820"/>
    <dbReference type="InParanoid" id="A0A1E5R820"/>
    <dbReference type="OrthoDB" id="541883at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004314">
        <property type="term" value="F:[acyl-carrier-protein] S-malonyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050858">
        <property type="entry name" value="Mal-CoA-ACP_Trans/PKS_FabD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42681">
        <property type="entry name" value="MALONYL-COA-ACYL CARRIER PROTEIN TRANSACYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42681:SF1">
        <property type="entry name" value="MALONYL-COA-ACYL CARRIER PROTEIN TRANSACYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ83034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="320" mass="36465" checksum="4F94D249B3EA7E8A" modified="2017-01-18" version="1">MSLKRLVTFHGQGSIIDFSKVAFNNHNINHLYETQYKTYCEQFPNLPQSLVILSHLLYKDLVSSGKLNPQHEETETIFLGHSLGELQSFVNGNDLISLKDAIDIANYRHLLMKQQVAKYQTFLESSHTPNTASNEMQMVALLLTKKFNLANPDYISSLQTHLAQEYQTLAVSNINTESSVVISGTKQELDKFLSSSTAQPNFNVPKKYKXLENPDKIAFHNNKILKHLQEPLYDYIWNKLKDNKTHTVSLLNNKVITNYQGKLVSQKDDLLENFVLQSCNTVQFTKCCKTVENSNIDEFTHVGPVSSVLQKMLQRNLGMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA52</accession>
    <name>A0A1E5RA52_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Nucleoporin GLE2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3011</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat rae1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RA52"/>
    <dbReference type="FunCoup" id="A0A1E5RA52">
        <property type="interactions" value="1299"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA52"/>
    <dbReference type="InParanoid" id="A0A1E5RA52"/>
    <dbReference type="OrthoDB" id="256303at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000190">
        <property type="entry name" value="Nuclear pore complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10971">
        <property type="entry name" value="MRNA EXPORT FACTOR AND BUB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="90"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="179"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="340"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ83778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="440" mass="47723" checksum="660BAA3DF961E5B2" modified="2017-01-18" version="1">MSFFGSTLNNNNATNSPLGSTMNNGLGGGMNNTMGGMNSMNNTMGGMNGMNTMGTNTLGGGFNTMNTMNANTNTNFGSLVPELANDITIANPASDSVSDISFNPQQDLIFSSSSWDGTVKIWDVQTGVPQERSQYVHNAQTNTPVLSTRWTDDGSKVASGGCDNNIILYDIMSQQQQVIGTHDQPVQAIRFAQVGPTNQSILVSGSWDKTIKYWDLRQPQPVCTVQMPERVYCMDAKSKLLVVGTAGKQVAVINLDSPQTIFKTMVSPLKWQTKSIACFNQANGYALGSIEGRCSIQYIDPEEHRKVGFSFKCHRPNTQTVGGTSSSLGSASSGPVGKSSPAYVYAVNSISFHPTYGTFATAGGDGSINFWDKDLRHRLKGFPSFKAPISVGTFNRSGSIYAYAISYDWAQGHNGRKPEYPNVIRLHQSSDDEVKERKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA62</accession>
    <name>A0A1E5RA62_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">aspartate--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g2839</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tRNA(Asp) + L-aspartate + ATP = L-aspartyl-tRNA(Asp) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9660"/>
            <dbReference type="Rhea" id="RHEA-COMP:9678"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.12" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RA62"/>
    <dbReference type="FunCoup" id="A0A1E5RA62">
        <property type="interactions" value="1231"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA62"/>
    <dbReference type="InParanoid" id="A0A1E5RA62"/>
    <dbReference type="OrthoDB" id="372395at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04320">
        <property type="entry name" value="AspRS_cyto_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000013">
        <property type="entry name" value="Aspartate--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02075">
        <property type="entry name" value="Asp_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004523">
        <property type="entry name" value="Asp-tRNA_synthase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00458">
        <property type="entry name" value="aspS_nondisc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003483">
        <property type="entry name" value="PRK05159.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450:SF1">
        <property type="entry name" value="ASPARTATE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450">
        <property type="entry name" value="ASPARTYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="12">
        <location>
            <begin position="262"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="45"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="54"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ83796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="570" mass="65643" checksum="5A57B641D13A035B" modified="2017-01-18" version="1">MSSEEVPVAKPAEASVEKPVEEPVILGEDGQPLSKKALKKLQKEQEKLKKKQERAKQLEEEALAKKKKQEEEANNDVSKLQNLYGKLPLIQSTSKTGLPRTSFKELLQPEYVNDESKLHIFRARVHSSRQQGATLSFLTLRQQENLMQAIIKVDLKDEQYPISKHMLKWVSSINLESIVLIHGYIKKVEEPIKSCTVQDYEIQIKKIHTISEVEPNALPILIEDCSRSEEEAEKLGLPVTNLDTRLDSRVIDLRTTTNQAIFKIQAGICALFREFLIEKGFLEIHSPKLLGSPSEGGSNVFEVTYFNKKAYLAQSPQLYKQQLIAADFEKVFEIAPVFRAENSNTHRHMTEFNGVDLEMAFEEHYDEVLDVLSEMFIFIFTNLKKRYGNQIDIVRKQYPMEDFLIPEKMVRITYKEGIKMLRDAGKTELGDYDDLNTECEKLLGKLVRDKYNTDFYILDKFPIAIRPFYTMPCPEDPNYSNSYDFFMRGEEIMSGAQRIHDADLLKERMKFHGLSPLDPGLKDYVDAFTYGCPPHAGGGIGLERVLMFYLDLKNIRRASLFPRDPKRLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA84</accession>
    <name>A0A1E5RA84_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">26S proteasome regulatory subunit RPN2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2926</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the proteasome subunit S1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA84">
        <property type="interactions" value="1442"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA84"/>
    <dbReference type="InParanoid" id="A0A1E5RA84"/>
    <dbReference type="OrthoDB" id="261572at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000017">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016642">
        <property type="entry name" value="26S_Psome_Rpn2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002015">
        <property type="entry name" value="Proteasome/cyclosome_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048570">
        <property type="entry name" value="PSMD1_RPN2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040623">
        <property type="entry name" value="RPN2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943:SF2">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01851">
        <property type="entry name" value="PC_rep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18004">
        <property type="entry name" value="RPN2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21505">
        <property type="entry name" value="RPN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015947">
        <property type="entry name" value="26S_Psome_Rpn2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 10" id="KW-0647">Proteasome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="26S proteasome non-ATPase regulatory subunit 1/RPN2 N-terminal" evidence="9">
        <location>
            <begin position="6"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="26S proteasome regulatory subunit RPN2 C-terminal" evidence="8">
        <location>
            <begin position="761"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="819"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="825"/>
            <end position="853"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ83820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="949" mass="104590" checksum="3407F769CB5C2565" modified="2017-01-18" version="1">MTSTANASSYLSLLKDDDRSVQLYALKAINEIVDKSWSEISNDLTQFEEIYEDPQFPDSKLAALVSSKLYYNLGEAESAVKYALAAGDYFSIDSQDDLFVETIVSKSIEMYIAQSTYNFEHANNAKPIDSKLESVFERMLQKCIDAEEYKLALGIAIDSFRIDIVNKILKERMQADGESSSVKLINYVLSCAITTVVSTPFRNLLLSNLFDSLINFTNPDYFILAKITVNLNKYEMALQMFEKLTHSSDENSKYLSYQIAFDLVNAASQKLLESLVTAFKQSSTTVDPKLIHILSGIPTCDYYNTFLLKNRNIDIALLDKAKTVMDGKFSLYHNAVTISNGFMHCGTVDDGFVRNNLPWLGKAKNWAKFSATASLGVIHKGNLLDGKKVLTPYLPSSRNPSRYIKGGSLYGLGLVYAGFGGGEITSYLKQIIQENSANTEDEGVAILLHGASLGLGLSAMGTANEELYDLLTEVLYADSATCGEAAAYGIGLVMLGAGSEQQITDMINYAKSTAHGNITKGLATSVALMSYGXQELSDAXVELLLKSENDSLRYGAAYTIGMAYAGTGENKAIKKLLHIAVSDSNDDVRRAAVTCLGFVLIRDYTTVPRIVELLARSHDAHIRCGTALALGISCAGRNLDSAIEILETLSKDPVDFVRQNAYIALAMVLIQQTEKTNPKVKKINETFISVMGNKHQEALAKFGASVAQGIMNAGGRNVTIQLENNEMGTLNTSSVVGLMMFCQFWHWYPLAHFMSLCFQPTTVIGVRGEDLVIPKFKFNCHTKEGIFDYPTAFEESPTAVAEKVATAVLSTSAKAKVRSKKHIGNKKDKEHSNVEKQEVKEEDKKDVXQGDVKETDEDDNQIKYVANSFKVPNCSRVLPAQLKYISFSKDERFVPVRKFKGNNGITVLLDKTPGEPVKITKTFKQQKDVSAPVPAPFKLEEELNFEDLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAH9</accession>
    <name>A0A1E5RAH9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Signaling mucin MSB2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2576</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAH9"/>
    <dbReference type="InParanoid" id="A0A1E5RAH9"/>
    <dbReference type="OrthoDB" id="3366093at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005034">
        <property type="term" value="F:osmosensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000282">
        <property type="term" value="P:cellular bud site selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006972">
        <property type="term" value="P:hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007232">
        <property type="term" value="P:osmosensory signaling pathway via Sho1 osmosensor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001402">
        <property type="term" value="P:signal transduction involved in filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039295">
        <property type="entry name" value="MSB2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35778">
        <property type="entry name" value="SIGNALING MUCIN HKR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35778:SF1">
        <property type="entry name" value="SIGNALING MUCIN HKR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184670" description="Signaling mucin MSB2" evidence="3">
        <location>
            <begin position="25"/>
            <end position="1516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1233"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="601"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="881"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="931"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="977"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1180"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1290"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1472"/>
            <end position="1501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="601"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="693"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="725"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="769"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="881"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="931"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="945"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="985"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1180"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1290"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1472"/>
            <end position="1482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ83920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1516" mass="157328" checksum="CAB1B3198218BEF8" modified="2017-01-18" version="1">MLAKLNLIQKALVLFVLFTSCISALDQENNFSLTSTHTSTLKPLSKEKRAWYNQYYRSSDTSNTSTPTTTTTTTTPFTTTSITPSSPTTATTSDSEYWYPNYTGPGWYYINGDYVFKMSSFKVASTDDISPSTTSTLSITSAYSSSSSSTEEASSTTPAFTSTTEEATSTTPSTTEEATSTSSSTTEEATSTTPTFTSSSEEATSTTPSSTEEATFATPSSTEEATFATPSSTEEATSTTPITTSTTPTFTEEATSTTPTSTEEATSTSPSTTEEFTSTTSSTTEEATSTTPTSTEEATSITPTSTEEATSTTPTTTSTTPTSTEEATSTTPTSTEEATSTTPTTTSTTPSTTEEATSTTPTSTEEATFTSPSTTEEFTSTTPTSTEEATSTTPTTTSTTPSTTEEATSTTPTSTEEATXTXPSTTEEXTSTTPTSTEEATSTTPTTTSTTPSTTEEATSTTPTSTEEATSTTPTXTSTTPSTTEEATSTTPSTTEEATSNTPTTTSTTPSTTEEATSNTPSSTEEATSTTPTTTYELSTSDSITPTSVSTSSSYVAPTSALFSSSTPTPTTATSSETTAFDLSATTSSEASDYSTIIYSSTTSSSETTSATPTDETTSTPIESNTTTPPSSDTFSTSESSYVSPTVSSSTFEPTDAPTPTSTSESSYSSTAFSSEPVYTPSTSTTSEPAYIPTSTTSESVYTPSSTSSESIFIPTSTTSEPAYTPSSTSSELVYTPSSTSSESTFIPTSTTSESVYTPSSTGSELVYSPSSTSSESTFIPTSTTSESVYTPSSSSLEITSESSTSSSTFENTLSASFTSALSSVTQLSSETSSTFSSSSDSDIFTNPLSLASTSSESASSIETSSTLLSSSSAEVTSETASSAVSSSSSMLTSESTTESFTDSSISVSAPSTSNPIVLLPTSTLQVATSAPSVSETKTLSSVPTSEATHTTTETSNNWLPSSLLLFSSADASNDASSKIDTKTLKPTGTKTTSQTGSHSTPINFSSVTQNLPQVIEAPATMTGTATASAQSGSATSTGYDDSKYEKIAVGFKQSLNYEFVVQNPYSSAQIFAYLPALLTYPFTGVSGEDVSADDLALFNDVTVQDLVPYTSSDFSYVVTLAEVIFPKNYVSKLQALVTNTDSILYKEPEKIIQQLASQIDPSIPLTILINMNTALSSSSGSSNSNSSSNNKNGTSSGTSSDSGDGIGALGQGSTSYKGYSVKSFLAVKKNRIGFFIGVICGGILYIALMILLARWFIVVIRKRQPEQEQDKGTQAFGVDPFEKASYHSSXISAGSHSGSSNASAIGYDEKQQQPQHDSSLAMMMEKPALLYEMPDFDEEHFDEFNDQVSMTPSMKIENWMDYNMYGGAEGGVAAGASQQQKPSRSLRKNMSVHMNKKQPAVPPSSSSLIGTVNDSNWNAVSTNKAGSSVANSSNVGNVSPTKAATAVSFATGPVSSSASAAAAARLSGYAGSAVDSNNNNSNKKRSSVSTGGGANGKLKISKPIASSNSLGWNQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAL1</accession>
    <name>A0A1E5RAL1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative cardiolipin-specific deacylase, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2493</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAL1"/>
    <dbReference type="STRING" id="56408.A0A1E5RAL1"/>
    <dbReference type="InParanoid" id="A0A1E5RAL1"/>
    <dbReference type="OrthoDB" id="7457040at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042171">
        <property type="term" value="F:lysophosphatidic acid acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035965">
        <property type="term" value="P:cardiolipin acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886:SF23">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE ICT1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886">
        <property type="entry name" value="RE40534P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="111"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="321"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ83932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="519" mass="58614" checksum="A340E405CD5289BD" modified="2017-01-18" version="1">MSASTSSDTKPLQNKDPFKKPPVKENEKDLIETYGFFEAFHTWRNVSSLEKTEEKVLSMLPIFPESADPNIRAEIINTPIDYKVPGTSSGNYIHEFYIENNNSSASEDHEVVMVHGYGAALGMFFNNFESLGKLPGIKLHCLDLLGFGLSSRPAFPKRETPSNKWFSHFWKQNKHQPNFIMDEEYKVYTSLKDVENAENFFLDSLESWRIKKNLDNFTLIGHSLGGYLSCCYALKYPERVNKLVLVSPVGVETSVFDLGELEKQKKKQQSLGHDVQNAHKTIKIYTSSNEPVEKEELGPDVAKEMESTEALEAKKEHSENSENSENSAANNKNNNNNNNSSIKTSSSFLVPNEHGHVEKIPNVSGMLKYLWVKEISPFSVLRAMGPVGAKMASQWAFRRFSGLPDQQSVLLMHEYVYGGFKLPGSGEKCLTRILGPGALAYNPLLKRAPQNLQCDSLWLYGDVDWMSKEAGKVMVNKINETNLLGKKAQYKIIENAGHHLYLDNPDDFNSTLTKFLDFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAL4</accession>
    <name>A0A1E5RAL4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g2805</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 12">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAL4"/>
    <dbReference type="FunCoup" id="A0A1E5RAL4">
        <property type="interactions" value="694"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAL4"/>
    <dbReference type="InParanoid" id="A0A1E5RAL4"/>
    <dbReference type="OrthoDB" id="10056816at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055029">
        <property type="term" value="C:nuclear DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006363">
        <property type="term" value="P:termination of RNA polymerase I transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10507">
        <property type="entry name" value="Zn-ribbon_RPA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019761">
        <property type="entry name" value="DNA-dir_RNA_pol-M_15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034004">
        <property type="entry name" value="Zn_ribbon_RPA12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF14">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01030">
        <property type="entry name" value="RNA_POL_M_15KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 9 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="13">
        <location>
            <begin position="83"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="10">
        <location>
            <begin position="10"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ83944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="126" mass="13710" checksum="1D518A49E0B4CE86" modified="2017-01-18" version="1">MSVVGSLIFCLECGNLLDNPSSTTKDHIECSQCEATYPKKNFANLKVKTSTAPDAFPSKLSAKKSVVKTSLKKGELDGSGAIIKEKCPKCGNDEMQYHTLQLRSADEGATVFYTCTKCGYKTRTNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAW2</accession>
    <name>A0A1E5RAW2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein UPS1, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2962</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAW2">
        <property type="interactions" value="359"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAW2"/>
    <dbReference type="InParanoid" id="A0A1E5RAW2"/>
    <dbReference type="OrthoDB" id="341300at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006797">
        <property type="entry name" value="PRELI/MSF1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037365">
        <property type="entry name" value="Slowmo/Ups"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11158">
        <property type="entry name" value="MSF1/PX19 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04707">
        <property type="entry name" value="PRELI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50904">
        <property type="entry name" value="PRELI_MSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRELI/MSF1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="251" mass="28924" checksum="626A641888D673D6" modified="2017-01-18" version="1">MVLWHKNTHNYPNTNFPTFSYAFLNRYPNPFASHILSIDVLNTEFVNNTRLXFDNNSDFNSVQSKTXCRDNNILKITRIIKKSGKLPQWIKPFLGKINQSMIMEVLYVNKDNQTLQSYTKNLDHTKIIQVEEFTTYQYNTSSNSMDVSSKVKFTSGFNAYKATTPVANAPSIPTNKELITKSGQESKTELENISNINTNTTSPKRFSFLNLDLSSRIEQWSHTKFAENIKKTRNGMDYVLENITLNNETPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAY3</accession>
    <name>A0A1E5RAY3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2671</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAY3"/>
    <dbReference type="FunCoup" id="A0A1E5RAY3">
        <property type="interactions" value="93"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAY3"/>
    <dbReference type="InParanoid" id="A0A1E5RAY3"/>
    <dbReference type="OrthoDB" id="426235at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43885">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43885:SF1">
        <property type="entry name" value="SUPERFAMILY HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G13290)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ84054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="244" mass="28418" checksum="80112200CE3AED84" modified="2017-01-18" version="1">MISLHRALFKKKHKTYAKFFSHPFVTKNMSACKLIVFDMDGTLTLPQRWMFKEMRESINLPLDSKKDILGYLDNEVTDEKLRSQYHVNIKNIEKKAMLLQEPQPGLNELLAYLHSKNISKNIVTRNYIDPVQNFIAKFVHKDYNTFDVILTRSFKPPKPSPQPLLHIITNNINPETHGTDLNYKEFFMVGDSYDDMKSGRDAGFTTILVVNETNQKNILEHDHHKLLVDHTVTHLAEIINIIEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBB7</accession>
    <name>A0A1E5RBB7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein 51</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBB7">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RBB7"/>
    <dbReference type="OrthoDB" id="203678at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="209" mass="23783" checksum="87815EB11329D6A8" modified="2017-01-18" version="1">MSLSNPDNNNNNNNNNNNNDEINNSXSNVARKPQMIISHKRNNTANTNRLSEQQQQQSNDANLSRASEDAYKRKMDRQRLKEYYARLKEQNGKXAGSDAVPKVSVDNXEQRTDNAAGTDQTHTSKEPHXDSDILKHGRXFKELLHDYNTLKVQEVDGLKDIKTNIYDNYYDLVKINNLLQENKHKTAGSMDKLNALINDLKKGNTNTAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBG8</accession>
    <name>A0A1E5RBG8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyldiphosphate specific]</fullName>
            <ecNumber evidence="5">2.5.1.87</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g2896</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>n isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate = a di-trans,poly-cis-polyprenyl diphosphate + n diphosphate</text>
            <dbReference type="Rhea" id="RHEA:53008"/>
            <dbReference type="Rhea" id="RHEA-COMP:19494"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:136960"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.87"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the UPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.87" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBG8">
        <property type="interactions" value="287"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBG8"/>
    <dbReference type="InParanoid" id="A0A1E5RBG8"/>
    <dbReference type="OrthoDB" id="19639at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904423">
        <property type="term" value="C:dehydrodolichyl diphosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038887">
        <property type="entry name" value="Nus1/NgBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21528">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT NUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21528:SF0">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT NUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ84239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="388" mass="44586" checksum="BA80B7D8BE859043" modified="2017-01-18" version="1">MQRLQGIGERMQHTNKNNHELDGSLMNVNSSNSNEQIIRKRTGVPENDQHHESYNLSVGKLKXLTENTLLLNTSNGADTSKRAHLTSNENSWNKKFTETCLHILFNFLLATFYCIFAMIRYFQYVINRIKIKIFNLAYNPANTPQLIRRDVNSLSKIPKNLAAILEYKSEEDVGGGVLGLMDGSSDLVAWSLSSGIKHLVLYDYNGILKKDVDMLRYTIYNKLAKYFGPNNVPKYAIRIPRTDSIYYNTAEQEVEEEKXVDNADNNTNFKTETNNNKKVSIEITLLSNVDGRETVVELTRTMAELCYKKELDIKDVTMELVDKELIQLVGNEPDLLLYFGPNLDLQGYPPWHIRLTEFYWEPDNDEVTYSVFIRGLKKYATCKINVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBK0</accession>
    <name>A0A1E5RBK0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Syntaxin UFE1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2512</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBK0">
        <property type="interactions" value="292"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBK0"/>
    <dbReference type="InParanoid" id="A0A1E5RBK0"/>
    <dbReference type="OrthoDB" id="342981at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15959">
        <property type="entry name" value="SYNTAXIN-18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15959:SF0">
        <property type="entry name" value="SYNTAXIN-18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="328"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="260"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="227"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ84272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="351" mass="40362" checksum="9E7C63F91E3D0E2C" modified="2017-01-18" version="1">MADITNDFQIQVGKFPQTAAVIQSERKFQAPLDTFNKECYELWHHLQEVHYLVTEVEPLYLDEKAMSAXEKDEFDDEMRFQLQQYLEKLKFLENYEKQRTQSIIKKKNAISISNLFHKDPLKNLDTENYQMLLVQHRSGVLKSMQLKLIELSQKLGNLQSKRLALHQGALAVDFEQARKSAFSTANALHDFNGXPTELRSGLEKDVLTNSDEQDIAPLADVHQSPIIETTREEVQEYEQTMKQLTQEQIQLLESDHEDILNFKNQQLHEVENLNKTIMGIAVLQNELAIHLQTQSDTINTIMDNHDTVHVDILQANKNLKKAGRAGGYSAKMVSTMAIVFGLLILFLDFIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBK3</accession>
    <name>A0A1E5RBK3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cyclin CCL1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3022</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBK3"/>
    <dbReference type="FunCoup" id="A0A1E5RBK3">
        <property type="interactions" value="973"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBK3"/>
    <dbReference type="InParanoid" id="A0A1E5RBK3"/>
    <dbReference type="OrthoDB" id="340962at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20524">
        <property type="entry name" value="CYCLIN_CCNH_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20525">
        <property type="entry name" value="CYCLIN_CCNH_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031658">
        <property type="entry name" value="Cyclin_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16899">
        <property type="entry name" value="Cyclin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0195">Cyclin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="4">
        <location>
            <begin position="128"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="20"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ84278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="380" mass="44077" checksum="83C41B897D80A0E4" modified="2017-01-18" version="1">MKKTGFDQTAPTKRHASLEMTESTPSKDSSALKTSNENDEPQPVKRISDDDLYRNSTQYRLWSFSKESLQNKRKLLSADIAARFDSKIQSFIEKEGAQLSSDEIEGLQKEMVPITAHEFLQIVTLYAQKVQLFGTKLKLPTEVTSTAVVFFKKYYLENSVLDVHPKNMIFTCMFLACKAENYFINIESFAAKVKQPKESILMHEFPLLESLKFTLMVHHPFKSLHGFFLDIQSILYGKVDMQYLGTVYEKTKKILVDSILTDVAYLYTPPQITLAALLKEDEQLTLKYLELKLLGFNINKEVLNEAEQKRKEQHDKILQTVRECLREMDVSYMPSRDEAWRIDARIHYYLDPMAFIKRIRKQKEAQTSGEPAQKKVKVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBK6</accession>
    <name>A0A1E5RBK6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein SYG1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2746</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SYG1 (TC 2.A.94) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBK6">
        <property type="interactions" value="540"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBK6"/>
    <dbReference type="InParanoid" id="A0A1E5RBK6"/>
    <dbReference type="OrthoDB" id="9970435at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004342">
        <property type="entry name" value="EXS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783:SF103">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 53 MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783">
        <property type="entry name" value="XENOTROPIC AND POLYTROPIC RETROVIRUS RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03124">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51380">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="446"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="489"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="545"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="575"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="624"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="666"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="698"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="728"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="786"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="9">
        <location>
            <begin position="1"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="EXS" evidence="8">
        <location>
            <begin position="657"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="50"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="272"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="946"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="288"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="970"/>
            <end position="979"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ83953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="979" mass="113320" checksum="34581A04CDACE006" modified="2017-01-18" version="1">MKFAEHITESSIPEWRDKYIDYKLGKKKIKQCRKYLETVNKDLFSNNKSLNSKGFTSNNSSNNNNNNNNNNNSSSPRTFNNAFSSGAQALSETTSLLHNTRGKYTKEQLKCINEFINDWLVKCELKESNDFYLWELENVCNAKYQGLELQMKIYTKRKENGLFLRENHNEYYSGDENEELNHHIEQSFNGRPINHCPEQNYDAMGNIEYGPDGAKNYSKCYTDVSGSKGKPNTHPKIPLLARMRFKLQELDLMPSLPEFDHFPRLKKIFHGHLPSQQQQQQQKARRGSTLSSFPVNGCTSEDEVRSLSFLWKGQETFVISDDQASRQLSSAIVEFYLYLELLKSFRSVNIEGFRKIVKKFDKVCKTNELQSFLNNVLHKTDLYKDSIKENDQDSITKWERKLTQWYVYDLAKSTKDRKIRSRLLANSTIQANMDELSVHKSNLCSMHMFLSGLNIGLSSCIIGLALYYGSQSTVSSELHKKLLPIWGGWYLVALMSLLFQLDCYIWFKNKVNYRFIIFGEILPTSKFGTSLFHNDFASTRIPSHLYFSSTLLSLCCISSFFSLSLTKKLEPWITLYWVLLLIILTRPQFFVPYMGNFSISSFWNLKTLIRLTCSGLYPVKFSDFFLGDMVCSLTYSLSDIALYFCMIRKNPQPNSCQCGSSNSYSMGILTALPSYFRMVQCVRRYFDSGDSFPHLLNAGKYFLTICYFLLLMLFRIKPSSLDGVNIRLWFIIIATLNSIFSALWDVILDWSLFQSLNWSTFLLRKDLYIAGTKNWKNGRYPWXGRMVYYIAILLDFIIRFQWLAYIWFPSEIQQNAMVSLAIAFAEMFRRFIWMIFRIENEHVANVNLFKISAEIRLPYNTGSEMHALYMQNRKKDIPGNVEPVQSSAVSISDEVVEYAPTVQEPPLARSPPHYRPMRRGSTLEMVAKAIPWAHLADFQRDSLDTALQKSDAEQVDNTNGAEDDDASVRSAYNSDSDND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBT7</accession>
    <name>A0A1E5RBT7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MIOREX complex component 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBT7">
        <property type="interactions" value="118"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RBT7"/>
    <dbReference type="OrthoDB" id="3972851at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45613:SF9">
        <property type="entry name" value="MITOCHONDRIAL GROUP I INTRON SPLICING FACTOR CCM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45613">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="732" mass="85344" checksum="5A6E8F99B87F1B31" modified="2017-01-18" version="1">MLASRTAVRSFHFTCIKHETQLMTKLRKFEKNHVLLKKTDKELRKSQAYKDIKLKKQVKTPYTRQKVVQLLNERYKIPLESLKGVEIGPTSNQDVEVFRKISNNKEIFLRDKRLLFTILGVSGEQLRDAKLISDDVEKFLKRNQPEKALLLIRLSNTKKSVVAMNKLIEYYLVNRHDCSTALSLFNWRKKWGIYPNEESFTILFDGISKLPYKISQKNANNLINIIVGLIQNTNLDFQVNDIHFNSAMSALINSKDGKAIFELFDMRPKHVKKTAITYTILLKGLTSYYNTLMGEPRNAAVDSQNNLKNQVSDGKQLSLKEDTEQEKEALSTEAELNSHFDKYYRFFKGIVDQIPLKQMDDKLCYEMIYXLSRQGLLESENKLLKYKILSDCCNGLAHYFDFKGLLHEQRTPEFLPTWDKLLFSAHSKHEPQFKKFKLNQHVIALAIDLLGRNGSYKKMDELFQKMMLDATLNTRVTPLVLEQYFKNVLKGYTEVCDSKMLDVLNKCDEVGTRRVVSKEVQYLMNTAFLVKLQNAAVLRDXDKIISTFKNFFXYYDQYGSINTTKEYSDFTASDPSRKKWYIYFEMIKRAKFLERFGQDKRKLQKYFLDEYIKDMQKGVFEIANLEQQVNLNVVKFKKDIDLKAIMMLNHIIYDLDTQGSANFAKTHGTLLMHLKDYLLKRCTLMDVFLKSKTKVNFPISADPQPLKAPDFTFEQLDESIKSTLSSLSTKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBW2</accession>
    <name>A0A1E5RBW2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Protein ARG5,6, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">AWRI3579_g2856</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2 18">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 18">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 3/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">In the C-terminal section; belongs to the NAGSA dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">In the N-terminal section; belongs to the acetylglutamate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBW2"/>
    <dbReference type="FunCoup" id="A0A1E5RBW2">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBW2"/>
    <dbReference type="InParanoid" id="A0A1E5RBW2"/>
    <dbReference type="OrthoDB" id="438291at2759"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00107"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003991">
        <property type="term" value="F:acetylglutamate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003942">
        <property type="term" value="F:N-acetyl-gamma-glutamyl-phosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070401">
        <property type="term" value="F:NADP+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04252">
        <property type="entry name" value="AAK_NAGK-fArgBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23936">
        <property type="entry name" value="AGPR_C_ARG5_6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd24149">
        <property type="entry name" value="AGPR_N_ARG5_6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04263">
        <property type="entry name" value="DUF619-NAGK-FABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000019">
        <property type="entry name" value="Bifunctional acetylglutamate kinase/N-acetyl-gamma-glutamyl-phosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000029">
        <property type="entry name" value="Bifunctional acetylglutamate kinase/N-acetyl-gamma-glutamyl-phosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000011">
        <property type="entry name" value="N-acetyl-gamma-glutamyl-phosphate reductase, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00150">
        <property type="entry name" value="ArgC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004662">
        <property type="entry name" value="AcgluKinase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023013">
        <property type="entry name" value="AGPR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058924">
        <property type="entry name" value="AGPR_dimerisation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000706">
        <property type="entry name" value="AGPR_type-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041734">
        <property type="entry name" value="NAGK-fArgBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011241">
        <property type="entry name" value="NAGK/NAGSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006855">
        <property type="entry name" value="Vertebrate-like_GNAT_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00761">
        <property type="entry name" value="argB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01850">
        <property type="entry name" value="argC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342:SF0">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04768">
        <property type="entry name" value="NAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22698">
        <property type="entry name" value="Semialdhyde_dhC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036440">
        <property type="entry name" value="ARG5-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01224">
        <property type="entry name" value="ARGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51731">
        <property type="entry name" value="GNAT_NAGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 18" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 18" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="11 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="15 18" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16 18" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12 18" id="KW-0521">NADP</keyword>
    <keyword evidence="9 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="20">
        <location>
            <begin position="348"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="active site" evidence="19">
        <location>
            <position position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00072977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR036440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OEJ84385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="861" mass="94821" checksum="78416DCC262344A4" modified="2017-01-18" version="1">MSCAIRTLNPLKKASSKLLAPSALASFHINLKNGSTFSTRLYSNKVPTAVSKAGVGSFGTRSTVIQLLNNIGSKREVEQYLKYFTSVSQQQFAIIKVGGAIISDNLQELASCLAFLYHVGLYPIVLHGTGPQVNARLEAKKIEPDYIDGIRITNPETMAVVRQCFLEQNLKLVTALEQLGVRARPITSGVFTAEYLDKDKYQLVGNISKVAKDPIEASIKAGALPILTSLAETSSGQMLNVNADVAAGELARVFEPLKIVYLNEKGGIINGETKEKISVINLDEEYEELLKQSWVKYGTKLKIKEIKELLDYLPRSSSVAIINVHDLQKELFTDSGAGTLIRRGYKLIKRTSLKDFPSLDSLRQALQRNTDVSSGAISATAYLKQLETTPFTAYSDEPNDVLAIVYTDKEVPKLDKFLCTTSGWLNNVTDNVFASLKRDYAQLQWVVSEKDANIAWHFNKSEGSYLKDGKVLFWYGVEDLETVSKLINDFVSELSMTPFESSTSQPANAFKGQQKRLYSTHVKRTSPKIEGTNTSISKIALIGARGYTGRNLIELINNHPYLEVAHVSSRELCGKPLKEYTKDSITYENLSLADLKTLEKQKAVDMWVMALPNKVCLPFVEAINSATDGSSKIIDLGADFRFTDEKEWAYGLPELNNRTKISDAKRISNPGCYATAAQLSIAPLLDFVTGVPTVFGVSGYSGAGTKPSPKNDPENLSNNFIPYALTNHIHEREISTRLGTEVAFVPHVAQWFQGIHQTVSIPIKKGSLTSEQVADLYKNFYENEKMVKVLPAGEVELVKNISGYHGAVIGGFKVNEAGDRVVVCTVIDNLLKGAATQCLQNINLSLGYSEYEGIPSEKVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBX3</accession>
    <name>A0A1E5RBX3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ATP synthase subunit f, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2682</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBX3">
        <property type="interactions" value="94"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBX3"/>
    <dbReference type="InParanoid" id="A0A1E5RBX3"/>
    <dbReference type="OrthoDB" id="3969524at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019727">
        <property type="entry name" value="ATP_synth_F0_fsu_mt_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28161">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT F, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28161:SF1">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT F, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10791">
        <property type="entry name" value="F1F0-ATPsyn_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ84392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="100" mass="10675" checksum="E4DE54FC62E8C1CF" modified="2017-01-18" version="1">MIAKRALSTLIPPKIASPQNLTGGANNKLSNVIGFYKALPQGPAPAAAKPSGFIGKYKANFFDGENASGKPLWHLAVGLLTLGYTIEYVTHLKHHKGEXH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RC58</accession>
    <name>A0A1E5RC58_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2790</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RC58"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="unsure residue" description="I or L" evidence="1">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="1">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ84163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="285" mass="32814" checksum="371B07CA9BC3E2E6" modified="2017-01-18" version="1">MSRAVNTKILNSTLQKLNKNTXAKKDLVNSYKNLYSKVIPQTLMLNLQYYNTFLLWEMPSSTQEQHKLKXSLSTENLSXFSATQDANQITQAQPFIFEDQKFQLSHMDQAFSQLISGLPNDNFKVYDDAEAKHMFVTFQNISCVSDLQMMKKMLQSRVFSLGINMGDPLFSNIPHLLNHLNSLKTYRNSGNVLMYEKLLEIARKDREKNGTEKHQSLETEIEQNCKELLQTVGELFLNQLHLVNNGTIKGQRSKSLTSDYNWLIXEKSKQTVDKEEQQVGNSSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDP6</accession>
    <name>A0A1E5RDP6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S ribosome subunit biogenesis protein NIP7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1891</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Required for proper 27S pre-rRNA processing and 60S ribosome subunit assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with pre-ribosome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NIP7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDP6"/>
    <dbReference type="FunCoup" id="A0A1E5RDP6">
        <property type="interactions" value="1111"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDP6"/>
    <dbReference type="InParanoid" id="A0A1E5RDP6"/>
    <dbReference type="OrthoDB" id="27490at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902626">
        <property type="term" value="P:assembly of large subunit precursor of preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21146">
        <property type="entry name" value="Nip7_N_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21151">
        <property type="entry name" value="PUA_Nip7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.10:FF:000002">
        <property type="entry name" value="60S ribosome subunit biogenesis protein NIP7 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.220:FF:000001">
        <property type="entry name" value="60S ribosome subunit biogenesis protein NIP7 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040598">
        <property type="entry name" value="NIP7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055359">
        <property type="entry name" value="Nip7_N_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016686">
        <property type="entry name" value="Ribosomal_synth_fac_NIP7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005155">
        <property type="entry name" value="UPF0113_PUA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23415">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT/60S RIBOSOME SUBUNIT BIOGENESIS PROTEIN NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17833">
        <property type="entry name" value="pre-PUA_NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03657">
        <property type="entry name" value="UPF0113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017190">
        <property type="entry name" value="Rbsml_synth_fac_NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88802">
        <property type="entry name" value="Pre-PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PUA" evidence="8">
        <location>
            <begin position="92"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR017190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="178" mass="20213" checksum="21DF1BC18718552C" modified="2017-01-18" version="1">MRQLTEEETKVVFEKLAGYVGRNIQYMVNDTHVFRLQKDRVYYVSEQVAKFATSVSRANLMSIGICLGKFTKTGKFRVHITALQVLAEHAKYKIWIKPNGEMPFLYGNHVLKAHVGKMSEDIPEHAGVIIFNMHDIPLGFGVSSKSTSESRDLQPTAIVAFRQADIGEYLRDEDTLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDQ4</accession>
    <name>A0A1E5RDQ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2442</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDQ4"/>
    <dbReference type="STRING" id="56408.A0A1E5RDQ4"/>
    <dbReference type="InParanoid" id="A0A1E5RDQ4"/>
    <dbReference type="OrthoDB" id="3973260at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="484"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="557"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="133"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="559"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="603"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="612" mass="67742" checksum="3817BD5FEC3C09A3" modified="2017-01-18" version="1">MILDKFHSRSFKRQQSNSSANTNGNCNSTDEDVDMNTTITSETGSFHSNSNSNSNSNPNSNANSNSNSTSTTPSKDEMVSKSKFFSLSPRKKQNKMVQLASLTTKFESPMMNSAISPVFNNDSSSPNKATTQNKSNNNKSNNNNNNNNNNNNQSKINNSLFSFPSSSSSSSSSSLPYKQPSGKKKTNMFIQRPMSNSKGNSPLLAPSAAPQTPQFLMQNQLPSTPVFETPSSTPVFYKHKYQNSVTSLASSIDDNTPTTAYPPLNFSLPFIQLLLETYQEHCSNPTITPFDDTNPPYGVTNIVAKKALKRAIQKNIGLTITSNPLELNNNANVLAAIRKKLLNEVRKENVYESRNNSVISLSNVSASNIGFSNDLMWQQQQQQSQQKQFPSQAPIAQMPQPIMNFNENIAASSPLLHTSLAKNNNENQQFNNSNFQSNLQYLSCEGFNDDHDMNKNENELNSAFDPFSGQNGLYNNNNNSANRYANNNHTDLMSPLEPLPQQQQQQQQQPYGMMPPTSASSSNFMRPTFRSRNNSMLSELTRTRSNTTPHFYNAPPSAGSATFNNTGSPHNMFFNNVQVQSSHHNGLSETPDPKTSSYGSSNLKKDTQFSKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDU0</accession>
    <name>A0A1E5RDU0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulator of free ubiquitin chains 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1959</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDU0">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDU0"/>
    <dbReference type="InParanoid" id="A0A1E5RDU0"/>
    <dbReference type="OrthoDB" id="3640at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015063">
        <property type="entry name" value="USP8_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08969">
        <property type="entry name" value="USP8_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140856">
        <property type="entry name" value="USP8 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP8 dimerisation" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="287" mass="33135" checksum="6F7A8EE5D4880FC1" modified="2017-01-18" version="1">MAKSPKQLQEEASNYKFNKSISLQVYLTFCISLIEKAXILATNSTDTENAYIFYMRYLDLVMNKLCNHPDILISKTQSMQRDGSDVNXLHVKTYHQILRLEVPAILKIVEEMQKKIETRYQKEMAKHYIEPCKTHVKPLVKEQPEENHCTGRKSPDLNIEIKLPSTFDEHAFNNSIEYMKKSNLPVKSFNYKPANSLASPNNKEPELPSSFKRSNQFKVTADSHIQKKNANLMLNYNIPSLTTSEPSLDQKMVLRGSQFKVSRSFPXSKPTTKKMDFDYPELPNICI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDX2</accession>
    <name>A0A1E5RDX2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GTPase-activating protein GYP8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2396</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDX2"/>
    <dbReference type="FunCoup" id="A0A1E5RDX2">
        <property type="interactions" value="73"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDX2"/>
    <dbReference type="InParanoid" id="A0A1E5RDX2"/>
    <dbReference type="OrthoDB" id="206700at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045913">
        <property type="entry name" value="TBC20/Gyp8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20913:SF7">
        <property type="entry name" value="RE60063P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20913">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 20/GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="12"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="361" mass="41664" checksum="D720B7E700872196" modified="2017-01-18" version="1">MTSSEKDASKRSNSKETLRAIWRKELIENVKRDSKQNCRDLNETTTRQVQLDVKRSFSNITNKDLKQKYQRILQQLILNVLRRNVHFSYYQGYHDVASIFILLFTDIEKTESDSTLAVELELVSEMLYHFSLLYLRDFLMSTLDFTMDQLRVVPKIFAKLDTEIYETLFGNSDPKLVVSAPVSCLLTFFSHDLNNTEQIFDIFNTVVENKTMSWVIYIYCLIIVQNKKTLMKSVEATRDSFLNENDFVNINLQTTFYSKIDDTDWPDIFSQLNKQPKTTGDLPDSINQLSTLNNSKLGLFERSKVKLSLLEKIMKNEFKLNEKLHCTSSKNLASFSVSSTIKFSIAVGVSALIIGYISSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE27</accession>
    <name>A0A1E5RE27_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1901</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RE27"/>
    <dbReference type="FunCoup" id="A0A1E5RE27">
        <property type="interactions" value="1099"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE27"/>
    <dbReference type="InParanoid" id="A0A1E5RE27"/>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045143">
        <property type="term" value="P:homologous chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000635">
        <property type="entry name" value="Casein kinase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000538">
        <property type="entry name" value="Putative Casein kinase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="9"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="304"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="429"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="325"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="441"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ85126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="451" mass="52643" checksum="6CF5B56CE8F34D1B" modified="2017-01-18" version="1">MDLRVGKKFRLGKKIGSGSFGDIYHGTNLISGEEVAIKLESIDSKHPQLEYESRVYKYLSGGIGIPFMRWYGREGDYNAMVIDLLGPSLEDLFNYCYRQFSLKTVIMLAIQMISRIQYFHGRSFIHRDIKPDNFLLGNGRRASTVHIIDFGLSKKYRDFNTHNHIPYRENKNLTGTARYASLNTHLGIEQSRRDDLESLGYVLIYFAKGSLPWQGLKADTKRQKYDRIMQKKMEISMEQLCFGLPQEFVDYMKYCRNLNFDDRPDYLYLERLFKDLAVKLEYHNDHLFDWTLLRYTKTMLEQQQQQQQQQQQGQQQQGQQTQEQPSKRKDESFSKKVREITLQKFNAHFHYCDCTKKYHPSVEEIKGINVTGDVNTSTTPQNNNRMLPNTVMNGQRVLQQQLPGQNVSQNGLQNVSQNVPQNVPQNMQIPRHVPRQGGQNGQNTQGNDIWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE37</accession>
    <name>A0A1E5RE37_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA sliding clamp PCNA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2152</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Involved in DNA repair.</text>
    </comment>
    <comment type="function">
        <text evidence="7">This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processivity during elongation of the leading strand.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the PCNA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RE37"/>
    <dbReference type="FunCoup" id="A0A1E5RE37">
        <property type="interactions" value="1133"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE37"/>
    <dbReference type="InParanoid" id="A0A1E5RE37"/>
    <dbReference type="OrthoDB" id="534348at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043626">
        <property type="term" value="C:PCNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00577">
        <property type="entry name" value="PCNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.150.10:FF:000006">
        <property type="entry name" value="Proliferating cell nuclear antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.150.10">
        <property type="entry name" value="DNA Polymerase III, subunit A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00317">
        <property type="entry name" value="DNApol_clamp_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000730">
        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022649">
        <property type="entry name" value="Pr_cel_nuc_antig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022659">
        <property type="entry name" value="Pr_cel_nuc_antig_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022648">
        <property type="entry name" value="Pr_cel_nuc_antig_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00590">
        <property type="entry name" value="pcna"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF0">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02747">
        <property type="entry name" value="PCNA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00705">
        <property type="entry name" value="PCNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00339">
        <property type="entry name" value="PCNACYCLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01251">
        <property type="entry name" value="PCNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA N-terminal" evidence="9">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA C-terminal" evidence="10">
        <location>
            <begin position="128"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="259" mass="29119" checksum="8EAF8530ED408C68" modified="2017-01-18" version="1">MLEAKFSEASLFKRIIDSFKDCVELVNFVCDEHGIEAQAVDDSRVLLVVLKIEPSAFESFRCDKPVTLGLNLTSLSKVLRCGNNSDTLTLIADDEPDSIFVLFEDSKKNKISEYSLKLMEIDADMLRIDDMKYDCTINMSSVEFAKTVRDLSQLNDSFNVFVKKDAIKFVAEGDIGSGSVVLKPENDLEKPDERIEVHLSQPVELAFSTKYLLDIVKASTLSNQIIIKFASESPALFEFKLNSGGYLDFHLAPKYNEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REB4</accession>
    <name>A0A1E5REB4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chitobiosyldiphosphodolichol beta-mannosyltransferase</fullName>
            <ecNumber evidence="3">2.4.1.142</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Asparagine-linked glycosylation protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g2179</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man(5) intermediate on the cytoplasmic surface of the ER.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.142" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REB4">
        <property type="interactions" value="907"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REB4"/>
    <dbReference type="InParanoid" id="A0A1E5REB4"/>
    <dbReference type="OrthoDB" id="614844at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004578">
        <property type="term" value="F:chitobiosyldiphosphodolichol beta-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026051">
        <property type="entry name" value="ALG1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13036">
        <property type="entry name" value="BETA1,4 MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13036:SF0">
        <property type="entry name" value="CHITOBIOSYLDIPHOSPHODOLICHOL BETA-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ85237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="433" mass="49667" checksum="33F983888C4CED87" modified="2017-01-18" version="1">MWLLNLVLQKLPINKLKLTELEEISHLAKSKHTMHNAVIALILLWLATPVAAYVLVPKLFYGNKSTRKRIVIMVLGDLSRSPRMCYHALSFALTNLNQDKKGFGMDVEFCGYVENPLPHVIMDQDNINITPLPVIQNSKSFPFLLFGPYKVLLQIWYIAKTLWALRGSDYILLQNPPSIPLLPVVVLYKVFFTLNKTKLIIDWHNFGYTILKMKFNGSXSHPFVRIHYWVEYLFAKFGDYHLTVTHSMKDYLTLHWKLDGKKISVLHDRPGMQFKPLKDEKEKKHLIATSGFIKNYFPAFDXQKDKILVTSTSFTPDEDLSILLDSLVKWDRNSSXITLHCFITGKGPLKKFYQDKVASLKLSNVKINFVWLSNEDYPLLLQLCDFGVSLHLSSSGLDLPMKILDMFGSGLPVVAYGYPTLSKELVFEAEMAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REG5</accession>
    <name>A0A1E5REG5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Histone acetyltransferase type B catalytic subunit</fullName>
            <ecNumber evidence="4">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g2437</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HAT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REG5"/>
    <dbReference type="FunCoup" id="A0A1E5REG5">
        <property type="interactions" value="1170"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REG5"/>
    <dbReference type="InParanoid" id="A0A1E5REG5"/>
    <dbReference type="OrthoDB" id="10253098at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000114">
        <property type="entry name" value="Histone acetyltransferase type B catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.360.10">
        <property type="entry name" value="Histone acetyl transferase 1 (HAT1), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019467">
        <property type="entry name" value="Hat1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037113">
        <property type="entry name" value="Hat1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017380">
        <property type="entry name" value="Hist_AcTrfase_B-typ_cat-su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12046">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE TYPE B CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21184">
        <property type="entry name" value="HAT1_C_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10394">
        <property type="entry name" value="Hat1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="15">
        <location>
            <begin position="148"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="394"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ85309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="415" mass="48688" checksum="1BB5C8E85FDC4706" modified="2017-01-18" version="1">MDSFKPENWTASSNECVKISLLNSSTDKITTFNPSFTYPIYGDSEQIFGFKDLELILAFESKTFLPLVNIKYSEQLEKDKIGNVESNPLDTLLEYLPKSAENLVIKDEKKWVETFTNERDNDSVWKNFQKNGELIAKHSDTTKKNQSLHIYKMNISKVKDLHSRIQIFALFFIEGASYIDENDDDWDVYFLVNAETKDILGYATCYKYWFYQGAQQFDSSTNYKYRSKISQFLIFPPYQGKGYGSILYKSIYSYWLEQPNICEMTVEDPNESFDLLRDRNDLQYLIEHIDLNEIKLQVQKASLTQNFPSLCNLKLEKKQLHRLTEMLLLYLGKQSSARIMVKNRLFQKNYDALIELDELDRKDKLQSTFLTIEQDYLEIIRFVKNTQKLAKTHTVGDNQVKNGKEDGHQPKKQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REL8</accession>
    <name>A0A1E5REL8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Repressor ROX1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2173</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REL8"/>
    <dbReference type="InParanoid" id="A0A1E5REL8"/>
    <dbReference type="OrthoDB" id="6247875at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01389">
        <property type="entry name" value="HMG-box_ROX1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050140">
        <property type="entry name" value="SRY-related_HMG-box_TF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270:SF161">
        <property type="entry name" value="SEX-DETERMINING REGION Y PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270">
        <property type="entry name" value="SOX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="HMG box" evidence="5">
        <location>
            <begin position="246"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="3">
        <location>
            <begin position="246"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="340"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="429"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="493"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="538"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="580"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="659"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="754"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="822"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="25"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="61"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="101"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="506"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="538"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="580"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="599"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="659"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="754"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="782"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="825"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="865"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="878"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="897"/>
            <end position="910"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ85324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="910" mass="99777" checksum="AA2FC1E9B57726D0" modified="2017-01-18" version="1">MLPYTNHGNLSPQQQQQQMTMNSHINPQLQSLNNNSTSISSGGNGTFKSEDGDDNKTTSPTTISATGNTNTSNTTTIMTTPNNSNITNSNKLMNSATPIMNGSNSNGNNDGNGLTNTLIRPSSSMDSNVSNTNSNTNSNTNSNSNANSNANSNANSNSNAITNTNDNTITTSTNISNEGMNNGMNNGMNNGMNTGMNNGMNNGMNNGMNTGMNNMNGINNMNMAGLNEINTSTSKSTKKANQPNKIPRPRNAFILFRQNEHKFFINELNEQGKPVPHNSEISKIMGLKWRVLPQDLKYQWELKALQEKKEHALKYPNYRYKPLRKKGKGKLVQLMPLQQQQQQNGLTGSNSNSNTSTPNINTPNQQLLSGQSLQSLQNLQSLQSLQNFQNMQNFPQNDILNQMNGLNGIGNLNQLQQFQQFQQLHQLTNGNGTNSVNNTNNNSNNTDFTDLNNPLNRIQMSQLPLLQQQMQLQNKFQLQQQQLQLQQQQQQQQQQQNDDVTVAGSKKGRKKGTKKNTKKNNANEMVTDMTMNMNGAGIINTNKNGNVNFSESSQPPRVLKKRGRKSKAELEEMRLMKLQKEQHQHLETMEQQHLHTPTQQQQQQQQQQQLHGNSANGLLGSGDLPANMDLNGALANTSSSGAANAHSYPQHQQYNYLSQYQQPSQAQQQQQQQQQMNASGASNGGGSGNGSSNSLNPGYLNEQSSQHLYGSNGANQGYGYGSYYYQQQQNQPQNQGQNTQQNQQYDYFRYMSQANSSGNSANNGTNNANTSGVSPNAHQNGSSTQATSPANASSNTNASANTSANANANANANANSTFMLPPLKAQNSWNPTSAFQNYPQGSPSQVFXNQQNQDGVNRNASPVQSGSTSGSTTGSGKANKNAQNYPYQNVQQGIPPQYHQYQLQQKSQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REM6</accession>
    <name>A0A1E5REM6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Secreted beta-glucosidase SUN4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2112</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SUN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REM6">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REM6"/>
    <dbReference type="InParanoid" id="A0A1E5REM6"/>
    <dbReference type="OrthoDB" id="5554151at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053088">
        <property type="entry name" value="Beta-glucosidase/SUN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005556">
        <property type="entry name" value="SUN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31654">
        <property type="entry name" value="SECRETED BETA-GLUCOSIDASE ADG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31654:SF0">
        <property type="entry name" value="SECRETED BETA-GLUCOSIDASE ADG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03856">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184720" description="Secreted beta-glucosidase SUN4" evidence="3">
        <location>
            <begin position="24"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="89"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="393"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="401"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="412" mass="44187" checksum="059FE466B005280C" modified="2017-01-18" version="1">MYVKMRLLSLLVLIAPXMHTALCILGMKKHNASSLTDSAVSMASRASESLLSAASGAIWNGSENXTSDAENNTEYTSNDYLRIKHEQRLKQAGHHTHNSKNTDQADTETAKEKQVAGKTVSNLFRRSGTCAFPSHEGMVAVQINGDNAGWAMHSDQFCSYGQWCPYACEPGKLMGQWDPSVTSYVYPGSQNGGLYCDESGTLKKQVSGNDYCYDGKGTVSVQNNAGSDVAFCQTVLPGNEEMLIPTLVQQGSNSVIAVPGPEYWASTAAHYYINPPGVSVSDGCVWGSTANPWGNWAAYVAGANMDSSGNTYVKIGWNPVYFEDSCPYKNDKPSYGLKITCPDGSDCQGLPCSIDPSKVGLNSVTGPNGEETNFCVVTASGNSKAVIEVFEVDSGSSSQKREHHEHKKPAGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RET3</accession>
    <name>A0A1E5RET3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribosome biogenesis protein MAK21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2127</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RET3">
        <property type="interactions" value="1040"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RET3"/>
    <dbReference type="InParanoid" id="A0A1E5RET3"/>
    <dbReference type="OrthoDB" id="28947at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040155">
        <property type="entry name" value="CEBPZ/Mak21-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12048">
        <property type="entry name" value="CCAAT-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12048:SF0">
        <property type="entry name" value="CCAAT_ENHANCER-BINDING PROTEIN ZETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCAAT-binding factor" evidence="3">
        <location>
            <begin position="664"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="568"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="774"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="941"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="73"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="89"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="173"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="243"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="575"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="775"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="785"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="942"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="953"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="978"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1048"/>
            <end position="1058"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1116" mass="127711" checksum="1AD08132B31C055D" modified="2017-01-18" version="1">MSGSPLNLSSLKDKIANKLSKENVNNSKKQDKIKTKKNAKREELAKEEQKRLEKKTKAITAETLQKPNDYVNRKVNNNNGSSSSATSSKTDKKSWKQEALELGATEEDLKLVEXLNDEDDFKSEQEFDGDXEVENEGNNKDNSLSNDLKSMMKQIGLSSFQVEDVPDTKEEEKVEEDVEEDVEVEEELPKEDVEEDVEVEEELPKEDVEEDVEVEDPEEDEEAEVSNQESEVEEETXELSSATKEKKEKKDGMITETSLVVTDKLLLSTTVPWYEIPLDSEATAQQNEPLDQDQIHKLFERGKDALMKDNDAYYNEFTKNSSQRKFMSQILNDGTLNDKISALTLLIQESPLHNLKSMDILVSYCNKKSRTSILQSLNAIKDLFLNNGILPPNRKLKFFKNQNLSMLINKKTLALWYFEDYLKKLFFQLLEIMEKLSHDPITHIRMTVLTHVFDFLTQSPEQEFNLLRLGVNKLGDIDNKVSAKTSFQLLKLQNNHPNMKQIXIDNVVDVALKKNEGYHTIYYAVLTLNQTILKKTEQEIANSLIKVYFTLFEKFLIHSSSFANTNSDKLDHKNSATSELQTSKSYETSKRKKNVKKGKKGGVSVKAVVKTEQEIIEEKNAKLFSAILTGLNRAFPFSDLPSELYEERLDTLFKITHSSNFNTSIQALSLIFQVISKKTTLNPDRYFKTLYESLLDPRLVTSSKQGIYLNLLYKSLKFDKDVSRVEAFVKRIFQICSHWSNIGAVAGMLYLVLQLLKIVPQIKNLLINSPTDYTYESDNEEEGEEEKKENSEKKQKYFSKDRQYDPKKRDPKFANADKSSLWEITHFLQHFHPTVQSYAKAFVGEESDEELSKPDLGLYTLAHFLDRFVYRNFKQKTTARGSSIMQPLGGQHTGSLLVKASDRVLNELPANTENWLSKKTQDIKPDEKFFHQYFSTKQSAIKKTDPKKKSSNADDEEDDELNEDEVWDALVKSRPDVEGEDEEDEDIDLDEEDFSMSDDEXESEVELDLGSDIDGGEEEEEKEGADVGQEDNDNEDEDDLDVQALISGEDDDADEEEAXFAAFSAEESSDDVEQPNSKKRSSSDQDNKKSNKRKRAIKELPLFASADDYAQYMNSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF67</accession>
    <name>A0A1E5RF67_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mannosyl-oligosaccharide glucosidase</fullName>
            <ecNumber evidence="1">3.2.1.106</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glucosidase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1751</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cleaves the distal alpha 1,2-linked glucose residue from the Glc(3)Man(9)GlcNAc(2) oligosaccharide precursor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N(4)-(alpha-D-Glc-(1-&gt;2)-alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + H2O = N(4)-(alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:55988"/>
            <dbReference type="Rhea" id="RHEA-COMP:12806"/>
            <dbReference type="Rhea" id="RHEA-COMP:14355"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:59082"/>
            <dbReference type="ChEBI" id="CHEBI:132537"/>
            <dbReference type="EC" id="3.2.1.106"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Glycan metabolism; N-glycan degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 63 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.106" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RF67">
        <property type="interactions" value="171"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF67"/>
    <dbReference type="InParanoid" id="A0A1E5RF67"/>
    <dbReference type="OrthoDB" id="14419at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004573">
        <property type="term" value="F:Glc3Man9GlcNAc2 oligosaccharide glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004888">
        <property type="entry name" value="Glycoside_hydrolase_63"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054491">
        <property type="entry name" value="MGH1-like_GH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10412:SF10">
        <property type="entry name" value="GLYCOSYL HYDROLASE FAMILY 63 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10412">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22422">
        <property type="entry name" value="MGH1-like_GH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mannosylglycerate hydrolase MGH1-like glycoside hydrolase" evidence="3">
        <location>
            <begin position="682"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="167"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="350"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="464"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="547"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="874"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="351"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="366"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="472"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="547"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="874"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="885"/>
            <end position="904"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU369107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1273" mass="146419" checksum="B20AA708F737610B" modified="2017-01-18" version="1">MANPELARLQQDKDKTHDWKKWGPYLSERSWGTVREDYSSDGNAWMDFDFVTSHARIYRWCEDGIFGVCDSEQKICMSLALWNGKDSILKEKYFGLSGPQGNHGEDVKELYYYLENVPTHSYMKSCYKYPFAKKFPYKELLQKNKERGYQDKEFEIYDINGLFYETNSSSSSSSSDNKDKHASGENDDIPLAATKSIEKDLSPTETXSGAKAPGSQTPXTPYVDTPYFDTFFEMCKNDNDDPEEIFFRITVYNRSSVKSNELYLVPQVFFRNTWSWGVYDPKQKKXKKKTDEMGNEYEELQSTGYEDGYEGEISGYKPIIEKYNENCLKITHRDEGVKYVVFAPSPGIFEGNEKDSDHQNAHYADDDIDNDNDNDNDYADDENAVDDIVPKLLFTDNESNLVKLFGEDQVNLSKYTKDAFHEYIIDKKEESCNFETEQGTKACAMYYFPSIPPQEYVTIRYKMTSHPPNSKQTEKPIAKSTKKQDAAASGAGNEEQTPKQEDIEAMMKNNPQLKTENTNPVSITTAASVDKTMQKKMDEYIVKGDSSTTVSTLTDSSPAAPSSTEEKSKDKEINAQIDDEFFEYFDEEEFDVIFDLREYECSVFYNSIINQDLDQELQKIERQAFAGILWSKQFYHLVQENWSNGDPNSKVKPLENRANGRNAEWKHLYCADVLSLPDKWEYPYFASWDTAFHCIPIAMIDPEFAKNQLDLMTREWYMHPNGQIPAYEWNFSDVNPPVHAWAAYRVYKIEKNMTHTKQGDRSFLEKVFQKLLLNFTWWVNRKDSLGKNIFEGGFLGLDNIGIFNRSEPLPTGGILEQADSTGWMAFFSLQMLNISLELAKENSVYEDIASKFFQHFILISDALSYDEDIDDFYKEEEEEEEXEEKEGXGEDKGGKQKDTVEFGGKKKSKKKAPAGSLWNPVDKFYYDAIAYNGGANKTQLPIRSLVGLIPMYAVLTLEPQILEKFPNFKKRIDWFLDNKPKLCERNIASITKKGTGDRLLLSLLTKDRLCAIMDRLLDEEEFLSPYGIRSLSKYHLKNPFSMHVNGADYSVSYLSGESNSGLFGGNSSWRGAIWFPTNFLIIESLERLFLYYGTSLKFEFPKKSGIFLNLAEIAEMLQHRLIQLFLPDVKTNTKVCYHDEGEPGKFMSNDPYFKEYIPFYEYFDGDNGRGLGASHQCGWTALVAKLIQDHGHTFLPFGTHSMNTQFARRSSSATFYDSRSNSIDESNISPEKFAKLRFRRRSSRSLVNLAGAYLDLSKEEVELNKLMNAKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFA9</accession>
    <name>A0A1E5RFA9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome biogenesis protein SLX9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2276</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in ribosome biogenesis. Required for normal pre-rRNA processing in internal transcribed spacer 1 (ITS1). May be involved in the movements of the replication forks.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with the 35S, 23S and 20S pre-rRNAs and with the U3 snoRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLX9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFA9"/>
    <dbReference type="FunCoup" id="A0A1E5RFA9">
        <property type="interactions" value="282"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RFA9"/>
    <dbReference type="OrthoDB" id="4068648at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028160">
        <property type="entry name" value="Slx9-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15341">
        <property type="entry name" value="SLX9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="121"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ85564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="230" mass="26268" checksum="A75D34169FEFEF14" modified="2017-01-18" version="1">MVAKNKRLRVKATSRNKQQQQQNDPSQYDISQHIENELSAFLKPQSENEKFLYQHKDTKKTKAESKSSSFKDKILKSNINDATNISKSALRRRKRKLKDDLKPKMNDLLDSLENEGFQIESTDASSDGVREHKHVGGNSDRSKITKITDNTNFAVAAPSFTHSKTSQQGAFKNTNEPSIRTQKGAKLLQQKENDHFKQVLGTKQFQTNTFASLREVIAMKNLQEQQSMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFB0</accession>
    <name>A0A1E5RFB0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phospholipid:diacylglycerol acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1741</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFB0">
        <property type="interactions" value="250"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFB0"/>
    <dbReference type="InParanoid" id="A0A1E5RFB0"/>
    <dbReference type="OrthoDB" id="190846at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003386">
        <property type="entry name" value="LACT/PDAT_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11440">
        <property type="entry name" value="LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02450">
        <property type="entry name" value="LCAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="727" mass="82045" checksum="DF7FEB5C950FDA81" modified="2017-01-18" version="1">MAGTQRRKKSKTNGSAXTRKSSKQNGTKNNGQNSPXMQDVFDDDTSTSTGFDSKSGRTDKTAQETPNDSKDDKQKLKELLNGTDKVEVXEHPSKSDNDEEENKKKKTLTIKAHKHXVQHKKRLRDRRRTFFVFGTLMGILVALYFGHKSSNMELPDSLFQFQFDNLVNYENFQDVLDSWNTNLAKIIPTNLMSKTSRGTDLTSDFGLGKQLRKEYNLTAKHPVIMVPGVITSGLESWGLTGDEECDSEQHFRKRLWGSYYMLKTMLFNKNCWLKHLKLDPITGLDPPNFKMRAAQGFEATDFFVAGYWIWNKVLQNLGTLGYDPNKMVTAAYDWRLAYLDLEKRDRYFSKVKEQIEMGYSLSEGQKSVLFSHSMGSQVAFYFLKWVEAEGEHYGNGGDGWCNKYIEAFVDISGSLLGAPKAVPALISGEMKDTVQLNELAVYGLESFFSRRERLDLLRTFGGIPSMLPRGGELIWGDHTHSLEDEQIELKCQGKKNKDSSAPCLITTPPPNSYGEFIKFSDDNSSFTITDSINFIMENSPGWLSNRIKDQYSFGVATSEKQLTENAKSHTFWSNPLEVALPNAPDMKIFSIYGVGNPAERSYVYRKENYTETGLNVTIDYDAQPAPVYLTDGDGTLPLITHAMSHKWKQGVSPYNPAGINVTIVELKHEPKKFDMRGGAKTAEHVDVLGSAELNEILLKIAAGRSDLIEEKLSTNLNKWTQNIPFPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFD8</accession>
    <name>A0A1E5RFD8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Syntaxin-8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFD8"/>
    <dbReference type="FunCoup" id="A0A1E5RFD8">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFD8"/>
    <dbReference type="InParanoid" id="A0A1E5RFD8"/>
    <dbReference type="OrthoDB" id="244190at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd15859">
        <property type="entry name" value="SNARE_SYN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="271" mass="31153" checksum="CC0B113CB28F321B" modified="2017-01-18" version="1">MEYAKLSYEIDKLKNYVEERNRLIDVLRIEPSNKDTIIFKKQLNSILQLIDEVPDLQQAEKYNDILASISGEDTHFTQEQKNLYHYNTEKLKQESELATKKALQEPKDFVKKVRFSDHNDVKENYDNSQQLTKLPFSDQGSQISGIINFAPYKDSQPVNDDQSAYSMMSNDELLAMQHDTIHDQDTLLESLSSTVHRTHDMSLGIHDEVEYQNDGLLVDLEGMVDATANTLNKAHTKLNRFNKMNRDSGGSYCRGALIFVLMILLVVLFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFE2</accession>
    <name>A0A1E5RFE2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional coactivator HFI1/ADA1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1748</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFE2">
        <property type="interactions" value="1177"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFE2"/>
    <dbReference type="InParanoid" id="A0A1E5RFE2"/>
    <dbReference type="OrthoDB" id="3970911at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22933">
        <property type="entry name" value="HFD_HFI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024738">
        <property type="entry name" value="Hfi1/Tada1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21277">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21277:SF5">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12767">
        <property type="entry name" value="SAGA-Tad1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="240"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="253"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="268"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="286"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ85597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="516" mass="58431" checksum="1B367C4BFAE6263F" modified="2017-01-18" version="1">MSVAKIIPKHTLTNLSVSQDTIDCASLIEELQALLGKQLWIRYAQLITLFVLGKRSRKELIQELKNRIIKSKNFHTMLSKQKSFIKLHNRLLLSILGNSLRLSKLGNGTSTSNGFKMMNSSGSGLDHLNDDDLDGEPDASGGLKSRTKIGAQQLKAQNSTMENYKQIVMSLPQFDRIRLSKIVKESGKRGFVVSKILQTRLNNIPKLPYASNPTVENLFQKITEKQLLYKKHGDLEMLKDDYDLDGTAPSETGDAKKKKDQNGKKGTSAGSGTRAANNKKTGPVSDTKEDEKDVQKKGMVEWSQEIVDSYQGLLCQELYSLPDNKLLMQRMYGIMRENGLIGELDKSCLDLVILALEKYMRNLIESGIDSIRYRRKKYSDLYDLDANGAYQEIGAFTSGLQAPAEEIEFAKEEKIPVQEYHSQQQQQLDIALTNEDIMDTLQVNPSLMDPGSIFYNMNINYNKNDDQMVVFNSRIDDMPLFANPEDKPSFIPVDERNEGSREELNWLLKDILEQXR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFF3</accession>
    <name>A0A1E5RFF3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cytochrome B pre-mRNA-processing protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2432</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFF3">
        <property type="interactions" value="146"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RFF3"/>
    <dbReference type="OrthoDB" id="2107880at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061671">
        <property type="term" value="C:Cbp3p-Cbp6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037653">
        <property type="entry name" value="Cbp6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28250">
        <property type="entry name" value="CYTOCHROME B PRE-MRNA-PROCESSING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28250:SF1">
        <property type="entry name" value="CYTOCHROME B PRE-MRNA-PROCESSING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20180">
        <property type="entry name" value="UQCC2_CBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ85313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="158" mass="17996" checksum="00FD19199583F9DC" modified="2017-01-18" version="1">MSVSNASAKQAAKNIIALIKELPDERIRHLVSFKDVQLQRFSKVAEVDLQATPKTKPTLEEIKDILNRTKSPLGLQKSMLKKFADTIPEEEMSEETILQQIRALKTLQSNKFKNLYXVGDKLYKPAGNPDYYQRILDELSGKKKETFMSAIRTVVFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFF4</accession>
    <name>A0A1E5RFF4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ferrochelatase</fullName>
            <ecNumber evidence="12">4.98.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g1999</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the ferrous insertion into protoporphyrin IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>heme b + 2 H(+) = protoporphyrin IX + Fe(2+)</text>
            <dbReference type="Rhea" id="RHEA:22584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="EC" id="4.98.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Porphyrin-containing compound metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the ferrochelatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.98.1.1" evidence="12"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFF4"/>
    <dbReference type="FunCoup" id="A0A1E5RFF4">
        <property type="interactions" value="694"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFF4"/>
    <dbReference type="InParanoid" id="A0A1E5RFF4"/>
    <dbReference type="OrthoDB" id="1323at2759"/>
    <dbReference type="UniPathway" id="UPA00252">
        <property type="reaction ID" value="UER00325"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00419">
        <property type="entry name" value="Ferrochelatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03411">
        <property type="entry name" value="Ferrochelatase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1400:FF:000003">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1400">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00323">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001015">
        <property type="entry name" value="Ferrochelatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019772">
        <property type="entry name" value="Ferrochelatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033644">
        <property type="entry name" value="Ferrochelatase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033659">
        <property type="entry name" value="Ferrochelatase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00109">
        <property type="entry name" value="hemH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108">
        <property type="entry name" value="FERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108:SF1">
        <property type="entry name" value="FERROCHELATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00762">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53800">
        <property type="entry name" value="Chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00534">
        <property type="entry name" value="FERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="6 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11 12" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="377"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="377"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ85625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="401" mass="45898" checksum="6BCBD399F4062A92" modified="2017-01-18" version="1">MLRSTFQKISTVKSVSPQFIRFNSTSKAPTGVVFMNMGGPGKINETYDFLYQLFSDYDLIPISKKYQPQIAKFIAKFRTPKIEKQYEEIGGGSPIRKWSEYQAQEVCKILDKTNPETAPHKPYVAFRYAKPLTHVTYEQMLKDGVKRAVAFSQYPQFSYSTTGSSINELYRTIKKLDPKRTIAWSIIDRWPTHKAVVDAFAENIEKKLLEFPENVRKDVILLFSAHSLPMDVVNTGDAYPAEVGATVYHVMKKLNFQNQYRLVWQSQVGPKPWLGAQTADMSEFLGGEKNKKLMFIPIAFTSDHIETLHEVDLGIIEEVEYRDNLKRCESLNGSPTFIKGLADLMKDHLNKNEWYSKQLPLDFVLGKSNDSFEHPSEVFGDFSKKPVSENQKDTVASQKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH45</accession>
    <name>A0A1E5RH45_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL29m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">54S ribosomal protein L4, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1207</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RH45"/>
    <dbReference type="FunCoup" id="A0A1E5RH45">
        <property type="interactions" value="242"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH45"/>
    <dbReference type="InParanoid" id="A0A1E5RH45"/>
    <dbReference type="OrthoDB" id="270763at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038340">
        <property type="entry name" value="MRP-L47_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010729">
        <property type="entry name" value="Ribosomal_uL29_mit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21183:SF18">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL29M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21183">
        <property type="entry name" value="RIBOSOMAL PROTEIN L47, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06984">
        <property type="entry name" value="MRP-L47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 9" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="19"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="201" mass="24060" checksum="FDB3AFE39D9ABEAC" modified="2017-01-18" version="1">MLKFRRELHTSANLLVRNKWTKPKPKPAPRTNVRPPTITSHHSGSLRITPPIPPSVKNLETSEYHPLWQFFSNKKYIRGQIELDEYSRPWTVAELRLKSFDDLHSLWYSCLKEMNVLRREIELFDNQKIGGNVEAYENVQKKVQTTLWRIRHTLSERQWSYEYANQMFNDDQKIKEFLETFESEENQDVIKRLNNSIKQAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHC8</accession>
    <name>A0A1E5RHC8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DNA-directed RNA polymerase I subunit RPA49</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1553</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPA49/POLR1E RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ85983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHC8"/>
    <dbReference type="FunCoup" id="A0A1E5RHC8">
        <property type="interactions" value="735"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHC8"/>
    <dbReference type="InParanoid" id="A0A1E5RHC8"/>
    <dbReference type="OrthoDB" id="532500at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009668">
        <property type="entry name" value="RNA_pol-assoc_fac_A49-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14440">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06870">
        <property type="entry name" value="RNA_pol_I_A49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ85983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="417" mass="47083" checksum="6311BCEF5D5256AF" modified="2017-01-18" version="1">MVQNIKIKEIHEDSSSVVANLFGGINVPKDTKFSLYKKKRSASALDEETNELQTVEANYVVHGENERLEYKADSMASDNSQYLLGVYNPESEEIELYKTPYVADTVVISKAKKHLKGPKIKQAKDVRISTMRNALGEAFGTKKAKKAIADLERNRIDSEKLSENAIDIVDAVSANVSNLPTREQLDDNIDRPTPACNVDATDVEQIYPVSSIIPKREMQFIRCNEMITEEDTSSKLEYLPYQKSPYITKKIAHFSDATPMKKWQLLYYLSLLLGVYENKRSNNKEKLMENLNSPPEALIDGVLERFTILRAGVFGRSKERSFTIDPQSEDKLLCYILCCILHLDNFMVEISPLAQELNMKPSRLVNLFRALGCLVKGATVGQAEAFNIPKSAAATYKIASLKVPFKLPQMTRRGRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHH5</accession>
    <name>A0A1E5RHH5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1466</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHH5"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ86316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="74" mass="8529" checksum="9CD9F7E576142380" modified="2017-01-18" version="1">MALDDGIWVPKIRAVRQDPKNITLNSINNCSXEPENNFDLGPEFSDSKKLNYNGVWCXGVWVYKEQMYEVVVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHK6</accession>
    <name>A0A1E5RHK6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitochondrial intermediate peptidase</fullName>
            <ecNumber evidence="4">3.4.24.59</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g1472</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.</text>
            <dbReference type="EC" id="3.4.24.59"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the peptidase M3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.59" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHK6">
        <property type="interactions" value="660"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHK6"/>
    <dbReference type="InParanoid" id="A0A1E5RHK6"/>
    <dbReference type="OrthoDB" id="17530at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06457">
        <property type="entry name" value="M3A_MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000055">
        <property type="entry name" value="Related to mitochondrial intermediate peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.10">
        <property type="entry name" value="Neurolysin, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033851">
        <property type="entry name" value="M3A_MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024077">
        <property type="entry name" value="Neurolysin/TOP_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804:SF79">
        <property type="entry name" value="MITOCHONDRIAL INTERMEDIATE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 14" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 14" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M3A/M3B catalytic" evidence="15">
        <location>
            <begin position="285"/>
            <end position="768"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ86365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="777" mass="88622" checksum="85198D7CFBDCA3F8" modified="2017-01-18" version="1">MKPGVAYVLRQAPVIRASARICVPFXXQLFLSKATYATATRAINKTEIQQVFDNDKYWKQIKDQQKNGSTGLFENQYVTSPEGLLTFSQYCLKLAQDLVDKMHNDHSHEGLRDYIRRMDELSNILCRVIDLSELIRSSHPDIKXVEAAQKVHEKMFEFMNILNTDLRLCNTLKAVLQKQHPVYAELSDEEIKVGTILLQDFEKSGNFMGNEKIKDSFIQLSQQISVVGQDFINNTDYAACNYVKINCAKLDQSGTPTAVLKVLPKDLTHKNYKIPVDSHFAYSLLRACPDESIRKQIWXSLHSCDKKQIQRLRLLLQLRSLLATTMNSPNFAAYQLAGKLAKNPQNVLDFLNSLCKNLKGKAENELRNLQLTTGKSLNDKSGAGEADNDLMKPWDKDYYVTLESIKNKKLYSDGIEKYFSLGNVMQGLSDLFSAIYRVKFVPVAPQNGETWNEEVRRLNVVDESNNDNLIGVIYCDLFERWGKTSNPAHFTVSCSRRIFENETFNSSLTQMGKETSSTTGKTYDYQLPVIALICNFQSVVKRATGKQISLLQLSDVETIFHEMGHAIHSMLGRTELQNVSGTRCTTDFVELPSILMEHFAKDPKVLSRIGKHYHTGEEIDANLLHKIFEHNNSFEYTEAFQQLKMAYLDQHLHNLNMVDPKNENVDIVAEYHKIENQFGILVDDSSNWIGKFGHLFGYGALYYSYLLDRAIASKVYHHLFKKDPFSPDAGEKFKKSVLKWGGAKDPWECLALCFDKPELSKGDEKAMKFIGEIKQDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHV5</accession>
    <name>A0A1E5RHV5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribosomal RNA-processing protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1623</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRP7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHV5"/>
    <dbReference type="FunCoup" id="A0A1E5RHV5">
        <property type="interactions" value="282"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHV5"/>
    <dbReference type="InParanoid" id="A0A1E5RHV5"/>
    <dbReference type="OrthoDB" id="5390at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032545">
        <property type="term" value="C:CURI complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040447">
        <property type="entry name" value="RRM_Rrp7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040446">
        <property type="entry name" value="RRP7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024326">
        <property type="entry name" value="RRP7_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13191">
        <property type="entry name" value="RIBOSOMAL RNA PROCESSING PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13191:SF0">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 7 HOMOLOG A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17799">
        <property type="entry name" value="RRM_Rrp7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12923">
        <property type="entry name" value="RRP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rrp7 RRM-like N-terminal" evidence="4">
        <location>
            <begin position="12"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA-processing protein 7 C-terminal" evidence="3">
        <location>
            <begin position="182"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="279"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ86499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="314" mass="36731" checksum="A1F67D40AC1FAC7E" modified="2017-01-18" version="1">MSSEEETAVSMMKNGFIVVPFKLPETDIYPVQAVHYLFVKKHDNKQNEEESNTLFLFNLPILTHLDVLKTNFNKILSKYETQSIYEKILYLDEFKLNEINLNELSSDYYDQTNSSSKNKRYLPHNTCLLKFVDATSLNNCLSSLRKYSAKSGSEKKHLVVWEGISQPSLKDFTNFYNPLPVDYLKTEISEALQDFEDRESKAIEDLNNASSLVDEDGFTLVVGKNTKNLNSIKRKLLHKNPLTKFNKVSKVPRNSGLAQSVQDKENKKVKLDFYRFQIRERKKNEINELLRKFKDDQAKIKEMKLQKRFNPYRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHW8</accession>
    <name>A0A1E5RHW8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SWR1-complex protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1281</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHW8">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHW8"/>
    <dbReference type="InParanoid" id="A0A1E5RHW8"/>
    <dbReference type="OrthoDB" id="4067990at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020195">
        <property type="entry name" value="SWR1_Swc7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17330">
        <property type="entry name" value="SWC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184776" description="SWR1-complex protein 7" evidence="1">
        <location>
            <begin position="26"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="17727" checksum="D2878312A56E010D" modified="2017-01-18" version="1">MQTICGRTIGYNIILLLLQIILERQQDIAKNKDGKNDITEFLTDPVIDDFVLNEFKSHKMFEVYKLDGTLQGVSFDTPKKKNQNHXKLTLRDLRTIVATLYKTPISTPVNENLTKNRPTLITLCDRYYALRIEQLSTDMLPKLREELKAAKVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI03</accession>
    <name>A0A1E5RI03_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">leucine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Leucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g1291</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RI03">
        <property type="interactions" value="521"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI03"/>
    <dbReference type="InParanoid" id="A0A1E5RI03"/>
    <dbReference type="OrthoDB" id="15954at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00812">
        <property type="entry name" value="LeuRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000002">
        <property type="entry name" value="Leucine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000003">
        <property type="entry name" value="Leucine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000100">
        <property type="entry name" value="probable leucine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002302">
        <property type="entry name" value="Leu-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025709">
        <property type="entry name" value="Leu_tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00396">
        <property type="entry name" value="leuS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740:SF2">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13603">
        <property type="entry name" value="tRNA-synt_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00985">
        <property type="entry name" value="TRNASYNTHLEU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methionyl/Leucyl tRNA synthetase" evidence="13">
        <location>
            <begin position="73"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Leucyl-tRNA synthetase editing" evidence="14">
        <location>
            <begin position="259"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="12">
        <location>
            <begin position="465"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="15">
        <location>
            <position position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF09334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ86549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="927" mass="105450" checksum="D2FB7BDC7E4F078C" modified="2017-01-18" version="1">MRRXVXNRALSLXGKRRYAAVNSASAHTDSXTXTIPTLTSLSAKWNKRVLKTDIKLVKLKKNSSDPSTQESKYILSMFPYPSGILHIGHLRVYSISDALNRFYKINGYKVIHPMGWDAFGLPAENAAIERGINPAEWTASNIAKMKEQMALMMIDFDWSREVTTCSPDYYKFTQKIFLAMFKYKLAYRKEAEINWDPVDKTVLANEQVDSEGRSWRSGAIVEKRLLKQWFLGITKYAHDLQHDLKYLNDWPEHVKTMQKNWIGESHGTQVQFLLKLDNSGLANGLENINKIVTFTTRVDTLFSVQFLAISTNHDLARICSKQDSNLANFIIKCKNDESLKESKEGYLLEGLKVIHPLTKKEIPVFVAPYVIDTYGEGAVMGCPGHDKRDYGFWLTNASPEHKNTILKSIEPLEGKEDSTPFVSTEGKLNDLCGTEIQGLSTAQXKFAITNKLRDLKLGEYSTQFKIRDWLISRQRYWGAPIPIIHCDSCGPVPVPEKDLPVLLPKVDTLVSKGNPLVSINEFKDCKCPKCGSAAERETDTMDTFMDSSWYFFRYIDPHNTKMPFSRKAADQHMPVDMYIGGVEHAILHLLYSRFVSKFLTELGLWPKGFEHHGEPFKKLVTQGMVHGRTYINPANKRFLKPDELDFSKNPLTPIIKETGIPASVAYEKMSKSKYNGADPQECIERHGADATKAHMLFQAPISDVLNWDESKIVGVERWLQKLLKLSSSFSKSTTISLNSEGIFDMSTLQLLRNDSEVKXHNEVQKLVTSITXSFSETLALNTVISDFMKLTNLLEDALKNSEISKDIHLKTIIKLINMIYPVTPCVSEEALSIICKTQKWDFENFARSWPDAEPIKASSTVKYNIVINGKMRFVFESDKNFASLDDRAIVEALCQRTEGQKYLKNENSEYKPFKKIIKKARVISFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIE8</accession>
    <name>A0A1E5RIE8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Pyruvate decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g1112</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>pyruvate + H(+) = acetaldehyde + CO2</text>
            <dbReference type="Rhea" id="RHEA:45484"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 1 Mg(2+) per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the TPP enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RIE8"/>
    <dbReference type="FunCoup" id="A0A1E5RIE8">
        <property type="interactions" value="1055"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RIE8"/>
    <dbReference type="InParanoid" id="A0A1E5RIE8"/>
    <dbReference type="OrthoDB" id="3970464at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004737">
        <property type="term" value="F:pyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000949">
        <property type="term" value="P:aromatic amino acid family catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02005">
        <property type="entry name" value="TPP_PDC_IPDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07038">
        <property type="entry name" value="TPP_PYR_PDC_IPDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000018">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000019">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000024">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012110">
        <property type="entry name" value="PDC/IPDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047214">
        <property type="entry name" value="TPP_PDC_IPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047213">
        <property type="entry name" value="TPP_PYR_PDC_IPDC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452:SF30">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE ISOZYME 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036565">
        <property type="entry name" value="Pyruvt_ip_decrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="15">
        <location>
            <begin position="5"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="13">
        <location>
            <begin position="201"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="14">
        <location>
            <begin position="405"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <label>1</label>
            <note>substrate; ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <label>1</label>
            <note>substrate; ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <label>2</label>
            <note>allosteric activator</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="471"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="473"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="477"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <label>1</label>
            <note>substrate; ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR036565-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR036565-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ86645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="563" mass="61472" checksum="8634A602CD0F925D" modified="2017-01-18" version="1">MAEITVGKYLFERLRQVNCNTIFGLPGDFNLSLLDKIYEVEGMRWAGNANELNAAYAADGYARIKGMACLITTFGVGELSALNGIAGSYAEHVGVLHVVGCPSTSSQAKQLLLHHTLGNGDFTVFHRMSANISETTAMVTDLAAAPSEIDRCIRTTYITQRTVYLGLPANIVDLMVPAKLLDTPIDLSLKPNDADAEKEVVDTVLELISEAKTPIILADACASRHNVKPELKDLINATQFPSFVTPMGKGAIDEQHPRFGGVYVGTLSDPEVKDVVENADLILSVGALLSDFNTGSFSYSYKTKNVVEFHSDHIKIRNASFPGVQMKFALQKLLSQIKPVVANYKPLPVPARKPANAEVADSTILKQEWLWNELGNFLQEGDVVLTETGTSAFGINQAHFPTDAYGISQVLWGSIGFSVGCCLGAAFAAEEISKTKRVILFVGDGSLQLTVQEISTMIRWGLTPYLFVLNNDGYTIEKLIHGPTAQYNNIQPWKHQKILETFGSTDGQSIEVRTTGEWKKLAACPNFNKNDKIRLIEVFLPEMDAPSNLVAQAKLTEAINAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN16</accession>
    <name>A0A1E5RN16_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATP-dependent RNA helicase DBP2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g711</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN16"/>
    <dbReference type="STRING" id="56408.A0A1E5RN16"/>
    <dbReference type="InParanoid" id="A0A1E5RN16"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="109" mass="11724" checksum="4BBC791F89A8D925" modified="2017-01-18" version="1">MPGNIEDYVHRIGRTGRAGSTGTAISFFTEANSKLGNDLIKIMREAKQDIPEELRRYERNSFGNYHPRYNGGGGFRGGRGGRGGRGGRGGRGGRGGYRGGNGGYGGNRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN80</accession>
    <name>A0A1E5RN80_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">40S ribosomal protein S16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g950</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN80"/>
    <dbReference type="FunCoup" id="A0A1E5RN80">
        <property type="interactions" value="821"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN80"/>
    <dbReference type="InParanoid" id="A0A1E5RN80"/>
    <dbReference type="OrthoDB" id="426865at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000007">
        <property type="entry name" value="40S ribosomal protein S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_uS9_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001749">
        <property type="entry name" value="PRK00474.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569:SF16">
        <property type="entry name" value="RIBOSOMAL PROTEIN S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ88346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="142" mass="15751" checksum="E29BA11247778CDE" modified="2017-01-18" version="1">MSTQSVQTFGKKKSATAVAHVKAGKGLIKVNGSPITLVQPEILRFKVYEPLILVGLDKFANIDIRVRVTGGGHVSQVYAIRQAIAKGLVAYHQKYVDEQSKNELKKAFTSYDRTLLIADARMPEPKKFGGKGARSRFQKSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN86</accession>
    <name>A0A1E5RN86_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Chromatin-remodeling ATPase INO80</fullName>
            <ecNumber evidence="15">3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">AWRI3579_g885</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">ATPase component of the INO80 complex which remodels chromatin by shifting nucleosomes and is involved in DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the INO80 chromatin-remodeling complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">The DBINO region is involved in binding to DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="15"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RN86">
        <property type="interactions" value="1141"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN86"/>
    <dbReference type="InParanoid" id="A0A1E5RN86"/>
    <dbReference type="OrthoDB" id="372624at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000022">
        <property type="entry name" value="Blast:Putative DNA helicase Ino80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001269">
        <property type="entry name" value="SNF2 family helicase/ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020838">
        <property type="entry name" value="DBINO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050520">
        <property type="entry name" value="INO80/SWR1_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685:SF2">
        <property type="entry name" value="CHROMATIN-REMODELING ATPASE INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685">
        <property type="entry name" value="HELICASE SRCAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13892">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51413">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="21" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="DBINO" evidence="20">
        <location>
            <begin position="696"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="945"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="1535"/>
            <end position="1689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="246"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="385"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="579"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="820"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1214"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="543"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="764"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="266"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="309"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="333"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="385"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="407"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="454"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="496"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="629"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="829"/>
            <end position="838"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OEJ88324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1710" mass="197548" checksum="EA88ED35D185D49C" modified="2017-01-18" version="1">MSLKDILNQEVVTTDPSGEIVMEPRSYQLEITENGNNSMMINGSGHINDQSLNKKDINTRVPINHAIPHKETQNKTYSDITSFQKHLKCLQKMDYNESVLQDWNFLNLQELELITIWNNQIKDILEENDTVNEDIFESSSISATTGTKTSGKNNIKPDIVDNSLQLHKGDKTKVNNVKDRDNSVDLLATNSHKGLDNLCDNMDYVNKEWGMYQQYLNRKQGVLEKACDDMISKHLPQVKKALKEREKWELEQSTNRKRKAMREQSVENEEDEEDGENEEEEEEGEDDDEEDENSLMMENESNSGDERSLQITQKTKNKLIPTTTLNGKELLIKPKKNKKSLKNNKRLNGNEISDSLSNKTNVSNLLKRKKLNDSTASLINFSKSKKLSSNGNVNSNIHSSYDSDVDGDIKNESRQTPDIHNLSKYFYGKGKKGKSNSHEDTLNNNGYLDKDEREENDEEGEEGEEEQEDDETQTVNGQPLRRSGRVAVTQEEKNRSVIKRMVSKQQKKASEERKRRFTQCVVSDFDESTKKVEIKITLKQHHMKMYKRHIREKEQTKKKEEKAAAAALAAAEAAKTAAATKASQKSKQGKQSKKTNKDKDELQLQQQDKQDLQSTNGKKGKKGKSTADDNTEDQGPDDLEDEDEDLNEEQTNIEIPPLDTTNNHFDTENLPRYGLKMNAREARAIQRHYDNTFFAIWKDLARKDSNKYSKTLQSMQQVRMQNTRKTASLVAREAKKWQMKNMRQMRDVQVRARRGIREMSSYWKRNERDEKELKKKREKELLERAKKEEEEKEKQRQAKQLEFLLTKSEMVSHFIAKKVKTSEHEKAHNSATNLTELANTPHDGDEERPNIELDLTGEIQAETQLHEIDFDNENQEMLDKRAMENASNILKKQQRSVQTYDYNEDAQDELNFQNPSTLGDSTLEQPKILACTLKEYQLNGLRWLNSIYSMGMNGILADEMGLGKTVQSISMLANLAENHNIWGPFIVVTPASTLHNWVQEISKFVPDFKILPYWGNANDRKILRRFWDRKHLRYNKDSPFHIMVTSYQMIVSDAAYLQKMKWQYMILDEAQAIKSAQSQRWKTLLSFHCRNRLLLTGTPIQNNMEELWALLHFIMPDYFNSIDDFNTWFNKDSKDGNMNGMNTDHLNRLHDILKPFMLRRIKKNVQSELGDKIEIDVMCDLTQRQKKLYNSLRSKMSSNYEEIERAAIGGAAIADEENNNTSNGSKNGGSSSTSDTKLINAVMEFRKVCNHPDLFERADVNSPFLFGTFGNLNSFLNDKDNLDLMYSSKNIISVNVPRLIYDEMILPNFENNHIDIKDHILQEKFNIFNSTFENVKYWSFLCLLSGFENPNELKKMSQAQLLQRVLSLFKNPKEVVARVEFLSGLQKQEFSKNTSNLLVPTKNDMLSYAQNIEPDSIVGNLLNVRKSYYNEHYTNNLKPAFSVVAAAPPIVPNVFNTKSLANTLDNVLFDDKLTQALSTIPASIQNDLVVNKNVKIEELPKSEMWPEPLNRNFSTRHISMPSMDRFITDSAKLKKLDQLLVKLKEEDHRVLVYFQMTKMMDLMEDYLTYRQYKHIRLDGSSKLEDRRDLVHDWQTKPEIFVFLLSTRAGGLGINLTAADTVIFYDSDWNPTIDSQAMDRAHRLGQKKQVTVYRLLCRDTIEERMRDTAIKKEQVQKVVMEGKKKNKKIQDAVQVANAGEKQIPQPIHESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNA1</accession>
    <name>A0A1E5RNA1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sporulation-specific wall maturation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g673</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNA1"/>
    <dbReference type="FunCoup" id="A0A1E5RNA1">
        <property type="interactions" value="138"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNA1"/>
    <dbReference type="InParanoid" id="A0A1E5RNA1"/>
    <dbReference type="OrthoDB" id="4070114at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184814" evidence="1">
        <location>
            <begin position="21"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="327" mass="35143" checksum="685FAFAC0CA178DC" modified="2017-01-18" version="1">MNPTSLFAYLFAIAITSASALPTFLDDNIHKRSFVPYAKKETQNVTGVLNTGGAETNVTSNSSIIVNPNSLNTTNITLYELNNVSSWANSTQVATNLTSLVITCAHSSLESTGFYFGLAYPNETVVISENTTLGLAVSKAWETWGRGTVVVDQWGYIYSAISYPSCPIGYVSWKSLKVTYYYEASLVSILTPSSPLYSWKNHTAFNTSTVTSNISSTVPIYYFNGSKSSNSSSSSIPGLNNLVYSVVVVGVENTTIANEFKMSSNTSMNPIVVFASNNGTGFIDQEELTVGGALGAGYLSPEKVSVLLSFALAAGLNTWEQLWSILP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNA9</accession>
    <name>A0A1E5RNA9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor Iwr1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g985</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNA9"/>
    <dbReference type="InParanoid" id="A0A1E5RNA9"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="21897" checksum="A7AE01DFFAA659A3" modified="2017-01-18" version="1">MSRPSTPIITLDYTNVIVQEEPDEDTMNYAPSAFVGNAPVEIGECIVLSAGQELLYKDELQKYIASHQDHPIFTMIECEPEDEEEGFDEEEEEEDEDEDEEDEEDYSGYAGHRDNGGSAFALYYVEEPVSSQRELYNANKHSSNYGFEDYSENASDGFDFLEDGSDGSVYTYTYSTYQVEELDDEVEDVDMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNJ5</accession>
    <name>A0A1E5RNJ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g645</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNJ5"/>
    <dbReference type="FunCoup" id="A0A1E5RNJ5">
        <property type="interactions" value="318"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNJ5"/>
    <dbReference type="InParanoid" id="A0A1E5RNJ5"/>
    <dbReference type="OrthoDB" id="534912at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019740">
        <property type="term" value="P:nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3430.10:FF:000003">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029:SF10">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="216"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="377"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="10">
        <location>
            <begin position="20"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="441"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ88469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="481" mass="52302" checksum="895F4E39A0165530" modified="2017-01-18" version="1">MSDTTVADPNTLYDKADTTFVAIAAMLVFLMVPGIGLLYSGLSRRKHALSLLWCSLCVLGLCAIQWYFLGYSLAFSHNTKGSGFIGSLQMGAGHNMLFWGKPSVVTTVPDVLFAMFQLMFACVTTIILCGGSCERARLFPLMVFMFIWLTVVYCPIACWYWNPTGWLATLGVLDYAGGGPVHMSSGAGALAYALCLGKRNDPLTQKGMPKYKPHSVTSVVLGTVFIWFAWFGFNPGSAGAATIRAWYSFYNTNLCAATCSITWLFIDYVRFRKWTTVGLCSGAISGLVVITPAAGFVPLQYSFVMGVIGAFVCNFGMDLKNLLRIDDGLDVFSMHGLGGLSGAVMTGLFTHKDINALSGVDAAPIAGGWLNHHYVQLGYQIAGACATMAWSFVITVILLTAMNYIPFLKIRLSEEEEELGVDATEIGEFTYEEGAMYIPEPVRSKRSVQQPEPMSAIDDNIVSDSSHSDIVHNNNEKPVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNN7</accession>
    <name>A0A1E5RNN7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g795</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNN7"/>
    <dbReference type="FunCoup" id="A0A1E5RNN7">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RNN7"/>
    <dbReference type="OrthoDB" id="4067310at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097196">
        <property type="term" value="C:Shu complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031783">
        <property type="entry name" value="Csm2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16834">
        <property type="entry name" value="CSM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="282" mass="32242" checksum="AFAB2AF20CF8DD43" modified="2017-01-18" version="1">MTTKKTQFVEELPYLSVLKTHNLTTNVIELTEKIFCQDRPAEDTWNDENETPEQKDEPKNENDSKNANKNNTAMYYIDSINDFPTNLLLKRIPLTEESEERVYRRLSVLKVFDLDDLLMNVMKTIQFVKSSHFNVAAAEAEAEAEAEAEAEAGDVNITKKQNTINQIVILINGIDVLWDSYSMHGLKQAKRKLNEIMVRIRSFRNLSTQCTIKTIFTFESIKNDNDQAGVLPLRSTHGLSDLVNKENTNIMKTVPVGPNSNKKRNIFVLSNYLLNFYADASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNP3</accession>
    <name>A0A1E5RNP3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g977</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Has a physiological role leading to 18S rRNA stability.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the small ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNP3"/>
    <dbReference type="FunCoup" id="A0A1E5RNP3">
        <property type="interactions" value="963"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNP3"/>
    <dbReference type="InParanoid" id="A0A1E5RNP3"/>
    <dbReference type="OrthoDB" id="278325at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000461">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1230.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1230.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001931">
        <property type="entry name" value="Ribosomal_eS21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038579">
        <property type="entry name" value="Ribosomal_eS21_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10442">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01249">
        <property type="entry name" value="Ribosomal_S21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002148">
        <property type="entry name" value="Ribosomal_S21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ88515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="77" mass="8608" checksum="785A283E6ACDC485" modified="2017-01-18" version="1">MFSDRKCSATNRIIKAKDHASVQINIAKVDEEGRAIPGEYITYALSGYVRTKGEADDSLNRLAQTDGLLKNVWSYSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNR1</accession>
    <name>A0A1E5RNR1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g891</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNR1"/>
    <dbReference type="FunCoup" id="A0A1E5RNR1">
        <property type="interactions" value="512"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNR1"/>
    <dbReference type="InParanoid" id="A0A1E5RNR1"/>
    <dbReference type="OrthoDB" id="10253736at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322">
        <property type="entry name" value="PKSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322:SF736">
        <property type="entry name" value="RETINOL DEHYDROGENASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ88529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="292" mass="32520" checksum="196F4F0FB5125B60" modified="2017-01-18" version="1">MPSTTTNYRLKMLERLRHLAEPGIAPEQDTVLITGGCGGLGRELVKRFGNANYKVVAVDICSKTLFEQIFDCKIQKPTNMYYFSCDITCATSLQQLKIDIENLKIGPVTVLINNAGITSNTPIKDFSSANPMSIEKIMNVNFQSCFGLIQLFLPGMIAANRGYIVNVASVLGILSPAKLAPYGASKGALINMHELLNAELSRSNHINNNINTLLVLPGQIKTNMFANINTPSKLFAPVLKTEELADNIYKAMMNKHKTLCTPYYVHYIPLIKNFDWPLTFMARFFSKMDQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNT4</accession>
    <name>A0A1E5RNT4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Axial budding pattern protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g773</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNT4"/>
    <dbReference type="STRING" id="56408.A0A1E5RNT4"/>
    <dbReference type="InParanoid" id="A0A1E5RNT4"/>
    <dbReference type="OrthoDB" id="41532at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006644">
        <property type="entry name" value="Cadg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05345">
        <property type="entry name" value="He_PIG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00736">
        <property type="entry name" value="CADG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184823" evidence="3">
        <location>
            <begin position="22"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="458"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Dystroglycan-type cadherin-like" evidence="4">
        <location>
            <begin position="24"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Dystroglycan-type cadherin-like" evidence="4">
        <location>
            <begin position="142"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="348"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="492"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="594"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="716"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="604"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="721"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="757"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="836"/>
            <end position="846"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ88542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="846" mass="90780" checksum="D3208E20C3E2B7BC" modified="2017-01-18" version="1">MLPIDQSYIIILLLLPALVIATPTEFFSLSNQLLPICVVDKQFSYQIAADTFKSSDSMTYEAYDLPEWISFDSGSQTFSGTPSSSDYSGSSSYDQFDVTLQATDSSGSTNSSFTLILMSSKDGNSVTIDPDFSLLDTLQEAGETNGDSSLVLTPHKEFSLTFDTSDFDSSSDSLTYYARQTNGHTPLPNWLFFDSSNLHFYGTAPSINSDIAPEMMFEISLIATAVAGYTSQQINFVMNIGAHQFTTSLQQTITLSANSSDSYAVSYDIPMNKIYLDGSPVNSSVIGSVDLSDSAPSFISLNNNGDNSYEVSGTVPSSYTTKTTDFYVQVYDLYGDAVYINFELQNSRSSSSSSSRMSSTTSSSSSSSSASSTQSSSSRSSSSLNSTSSSFSATKTSRSSSSSSTSYTSSQNATSMAHTFSTKTLSPSLSATASNSASSSAAAPLKNNSTSHKKNKHVTAIACGVIIPVFFILLCLLLLFIFYRKRKNNQQQQQQQDMEKANKNASPFTPPGGAGGTGTGASAAAAGGAAMGGAAAIAGARQDSSDYSSDDEGFDNEKQDRTLNDKNADALNNIPAAISAESALDNTVRQFLDHTDSSSANTDPRNHSDFDDLHNENMHDANVVFLPHSNSLDTSDMYSPIRNSYASSDSGNVTEKPRNATTAYYNAQPSNKESWRYVSDSLGKERNSMYTNNSSASAKDQRSSYHTLNSIGTNEFMNTELHPDSNIKNKDPSKSTLLNVRDSAFEPKTMPPAEATGKTSNVSTITPSSFPPGTNTLPDHNTNTIDSSGTLKQSSLFEDVPEQTENVTRPDPPAKRKSNLVKMNDMSSDIQVGDATKIKGEEIQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNV6</accession>
    <name>A0A1E5RNV6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptide chain release factor 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g905</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNV6"/>
    <dbReference type="FunCoup" id="A0A1E5RNV6">
        <property type="interactions" value="712"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNV6"/>
    <dbReference type="InParanoid" id="A0A1E5RNV6"/>
    <dbReference type="OrthoDB" id="3971717at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000004">
        <property type="entry name" value="Peptide chain release factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050057">
        <property type="entry name" value="Prokaryotic/Mito_RF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43804">
        <property type="entry name" value="LD18447P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43804:SF7">
        <property type="entry name" value="LD18447P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="Release factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prokaryotic-type class I peptide chain release factors" evidence="6">
        <location>
            <begin position="269"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="324"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="324"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00067174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="407" mass="46342" checksum="0B12D9E7E77D04CD" modified="2017-01-18" version="1">MLQRLIPLSTKLTLKSWPAKCPPQVFKRFSSFEPLHPSLLSRAVGYSKELRALEAEMNVGQTQGFDVEKEKKYSHLSSIVGSLNRYISKLEQYVELKELISADPSLREEAQAEIEEVLPDLNKSKNTLFNKLLPPHMFADKPCLMEIRPGVGGTEAMIFAQDLLDMYINYCNHHRWPYRLLSTTENEEGNGIIEGILAIDEPYSYDKLKFESGVHRVQRIPATEAKGRIHTSTAAIVILPQLQDSNDQKSSDAYERSFPAKDIRIDVMRARGKGGQHVNTTDSAVRITHYPSGIVVNMQDERSQHKNKAKAFAILRSRLAEKERVEKEQSERKARTDQVSSTNRSDKIKTYNFPQNRVTDHRCNFSLLDMENVLNGTKLDKMLDEMATFDNSEKAKQLLAEIASENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNY0</accession>
    <name>A0A1E5RNY0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Altered inheritance of mitochondria protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g593</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AIM6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNY0"/>
    <dbReference type="FunCoup" id="A0A1E5RNY0">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNY0"/>
    <dbReference type="InParanoid" id="A0A1E5RNY0"/>
    <dbReference type="OrthoDB" id="4153866at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051236">
        <property type="entry name" value="HAT_RTT109-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31571">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31571:SF1">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ88591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="537" mass="61226" checksum="CA0D2EA728C0E17D" modified="2017-01-18" version="1">MIRQTENPIEMSDNISVDNIPVAQEEQGGGAEEDEAAPLSQEAGKVINHASGVNKEFRIPEDEEFRISSSENEKSSIYAGFRKNLKKYRMLIISVVVITFFIVVDKYSLPLLVSSNNSSHTSNGTKNTNDSNIYSDEFGANKFNGAGHTGIALSGLKLLQYFDTNLDSISTKELNSKKSKNQKEDLTSKTNFVSSYYYDLFMSFLISSQNALSFEENKKTLVEILTRDVTPLNYVHSHNDYWRTLPVFDALMHGVNSIEADIWLNNSYVKNVSDDIDTDDESSNHKTMLAVGHNKDYLDPIHRNLENLYINPLQILLDEVNQMREKTTEKLNGLFHTDTGMPTILYIDFKQPELSETVYDILVNEYLGTPDFQKYLTYKDQETNQIVFNPLIIILTGDYPRYQDYKKYAEFVFLDLDIVELVQNNSQTATEIPPAIVVSTSLKKLLNHCQSEKNGNEKSVPLEIESFVEHHLVSLNEQELQCMTDSIGFVNALGLKTRIWGVPQYPVYLRNSYWKQLLIDMKSDYLNVDDLDGVLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RPE0</accession>
    <name>A0A1E5RPE0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein YTP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g580</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RPE0"/>
    <dbReference type="FunCoup" id="A0A1E5RPE0">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RPE0"/>
    <dbReference type="InParanoid" id="A0A1E5RPE0"/>
    <dbReference type="OrthoDB" id="4137487at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018827">
        <property type="entry name" value="YTP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31685">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_6G12730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31685:SF2">
        <property type="entry name" value="PROTEIN YTP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10355">
        <property type="entry name" value="Ytp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="252"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="279"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Protein YTP1-like C-terminal" evidence="2">
        <location>
            <begin position="138"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="408" mass="46061" checksum="0F24DCD7AADFEC19" modified="2017-01-18" version="1">MITLCASVLAMDMDMDSEEEFPRPNIVAAGSKTFHYLLTMFFLLLLPGLSTVFAFVHKIHSVVSFQFLVMCYSILDAVFLRFPDGDGVENRVSRGTAWFLMGYSILSFWLALCAIKTANNSKWHGYLGGLLLAQTVAGWMKCCMAPVALFGFCREIHTGQCLAHGLMGTAFVLYGFVYTLVLVVPKIARADYSQDYVDSWVMCIWGIFNTFTEHRWGREPWNHGDYQHTAMGIIWACGGFLGILLGRNKKRTFIPSLLIIFTGWAMSQHAQHLAISTKIHFLFGLILMVGGACRIVEISFILKDSNVVNEIQSFQYLAPFCLTCAGILFMGANEEQLILVLRLEADHSSYGLIVISAAFMVYLWMLLCLKLYVRLNLKNENGFMKSYENVDFELDDMVESESHTRNST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV11</accession>
    <name>A0A1E5RV11_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein bL34m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g386</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL34 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RV11"/>
    <dbReference type="FunCoup" id="A0A1E5RV11">
        <property type="interactions" value="181"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV11"/>
    <dbReference type="InParanoid" id="A0A1E5RV11"/>
    <dbReference type="OrthoDB" id="431691at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.3980:FF:000001">
        <property type="entry name" value="Mitochondrial ribosomal protein L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00391">
        <property type="entry name" value="Ribosomal_bL34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000271">
        <property type="entry name" value="Ribosomal_bL34"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01030">
        <property type="entry name" value="rpmH_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14503:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL34M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14503">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN 34 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00468">
        <property type="entry name" value="Ribosomal_L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ90762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="114" mass="12904" checksum="DE6EA6C6FFDD17EB" modified="2017-01-18" version="1">MLSPQTRFFSSMLMGSKTSSFTSAHHNSQNLLSATSITSAGTTLESSTNTVRTGSLLFNVGTLLGRRWKTRGNTFQPSVIKRKRKLGFLARNSSTRGRKLLERRKRKGRWYLTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV19</accession>
    <name>A0A1E5RV19_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative glutamate carboxypeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g320</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M28 family. M28B subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RV19"/>
    <dbReference type="FunCoup" id="A0A1E5RV19">
        <property type="interactions" value="143"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV19"/>
    <dbReference type="InParanoid" id="A0A1E5RV19"/>
    <dbReference type="OrthoDB" id="5841748at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08022">
        <property type="entry name" value="M28_PSMA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02121">
        <property type="entry name" value="PA_GCPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000008">
        <property type="entry name" value="Glutamate carboxypeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000101">
        <property type="entry name" value="N-acetylated alpha-linked acidic dipeptidase like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.40">
        <property type="entry name" value="Transferrin receptor-like, dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039373">
        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007365">
        <property type="entry name" value="TFR-like_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036757">
        <property type="entry name" value="TFR-like_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF46">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04253">
        <property type="entry name" value="TFR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47672">
        <property type="entry name" value="Transferrin receptor-like dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="4">
        <location>
            <begin position="201"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="6">
        <location>
            <begin position="392"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin receptor-like dimerisation" evidence="5">
        <location>
            <begin position="700"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="294"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ90780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="791" mass="88326" checksum="086706714DA89449" modified="2017-01-18" version="1">MLGEKNEDDGYKPINFSNLKSKHTAKTTSKNRFLKWALIAAITMYVVLFKLPLLLSFTSFINKTELPHDTSIDVSALLLNILKSSNYAGDWSKIYTAHPHLAGQGLDLVEFTQNKFAEYGLASWNETYEVYMNLPVENSLSLLEIPTSSDATKAGVRYLNSSEPRVVYEASLIEDPLKEDPTTIGDDLTPAFHGYSASGNVTAEYVYVNYGTKSDFKTLGELGVDFTDKICIVKYGSIFRGLKVKFAQEAGCSGVIIYSDPGDDYFKEADGYKPYPKGPARNPSSIQRGSVQFLSQVPGDPTTPGKPSVPGAKRTDPYKSIPKIPSLPMSFRSVLPILEKLNGHGIACSDIPDSTWCTSGGLSSFDYSTGPNPSYQLNLYNNQSYDIRPIYNVYGNLTGTGSQPGYILIGNHRDGWAGSASDPNSGSAVLLEVARALHEITLTTGWRPSRDIVFASWDGEEYGLLGSTEFGELHSKNLKGHCIAYLNVDVAVGGTKLNVQSSPSLNKLLRESMKSVTYEEADSETSLFDDFFSRNEKIGILGSGSDYTVFLEHLGIPSVDMGFTSGPEDPVYHYHSNYDSYHWMSTMQDPGFVYHNRLAQFLGASLVKLSERQVLNMYFEEYATEMIGYFEDLTSKIPISWLTYVPPKAEKTLAVLISETKEVFDGFHNYTITMDTRTDSLQFSADNPDSVPFWKRFGLKWRILGQNMKIQFFERLFIYKKGLTGRKWYKHIVYAAGKDTGYEGFAFPGLKEALDDQNVAEFYKWTKIIKKILNYKAKKADDEKMSSCHMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV52</accession>
    <name>A0A1E5RV52_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein AIM7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g467</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the actin-binding proteins ADF family. GMF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RV52"/>
    <dbReference type="FunCoup" id="A0A1E5RV52">
        <property type="interactions" value="101"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV52"/>
    <dbReference type="InParanoid" id="A0A1E5RV52"/>
    <dbReference type="OrthoDB" id="3919494at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071933">
        <property type="term" value="F:Arp2/3 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071846">
        <property type="term" value="P:actin filament debranching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034316">
        <property type="term" value="P:negative regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011171">
        <property type="entry name" value="GMF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11249:SF2">
        <property type="entry name" value="GLIA MATURATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11249">
        <property type="entry name" value="GLIAL FACTOR NATURATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001788">
        <property type="entry name" value="GMF-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="3">
        <location>
            <begin position="4"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR001788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ90830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="158" mass="18112" checksum="BF79863C58ADB385" modified="2017-01-18" version="1">MSTSLYQISPETKTRINKFRTATSRAEKLEHLVIKIQPKPSYEIVVDDQEDGEDGDDDDDDWGVEVNELKDLPEALPDNLPRFVLLAYPLVTKDGLKKTPLVLLYWKPPTVVSQEWKMVYAGCLEMVRSFTNPNKFLEVNNGLEDEDDVQDLKEQIEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVA3</accession>
    <name>A0A1E5RVA3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g388</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RVA3"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="277" mass="30492" checksum="0D57C90FFE6B341A" modified="2017-01-18" version="1">MDNRLTFSSSNNNNNSGITDENEAIAKVSNVKSLHLDNKDDGKFVANTTRKPLAVKGNSNLLGSFPKSQVFSKDTPSVQIKQTHKNGPTASKSSKFNRPTMKHKNPVTVTHTGSFMVQNNNGQTTDKHTSNGNGGYRIQKSLVRQKSLVLNDFEDGKLGHTEEGKENVLDRDELERAEMTNKLRAVFTKGNDNEEDEDGYVTEETDSGSDLEDGDLSLAFLKPPCNKTLFDLLKPQGHEQLAATTDAATTDAAXTDALPLLLLLVKNKKHSMRFMTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVC5</accession>
    <name>A0A1E5RVC5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g318</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVC5"/>
    <dbReference type="InParanoid" id="A0A1E5RVC5"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="114" mass="12915" checksum="D8EFF65BB57A46C6" modified="2017-01-18" version="1">MSSGTGQYGECNGIPQQSFVNNYSSSDSNLSTTTTSSIFSRNMEDYNLKLKEVENYYLSNLLEDEYIDSFTKLSLNPSETQTSNSIYSQELHQQQQAQQQAKTRTCTLQPWANS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVE7</accession>
    <name>A0A1E5RVE7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Transcription elongation factor SPT5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g558</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">The SPT4-SPT5 complex mediates both activation and inhibition of transcription elongation, and plays a role in pre-mRNA processing. This complex seems to be important for the stability of the RNA polymerase II elongation machinery on the chromatin template but not for the inherent ability of this machinery to translocate down the gene.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SPT5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVE7"/>
    <dbReference type="FunCoup" id="A0A1E5RVE7">
        <property type="interactions" value="1406"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVE7"/>
    <dbReference type="InParanoid" id="A0A1E5RVE7"/>
    <dbReference type="OrthoDB" id="28901at2759"/>
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        <property type="term" value="P:regulation of DNA-templated transcription elongation"/>
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        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="4"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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    <keyword evidence="4 7" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="NusG-like N-terminal" evidence="9">
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            <begin position="240"/>
            <end position="331"/>
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            <begin position="337"/>
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        <location>
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            <end position="804"/>
        </location>
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            <begin position="1"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="8">
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            <begin position="719"/>
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            <begin position="22"/>
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            <begin position="41"/>
            <end position="52"/>
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    </feature>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="1052" mass="114567" checksum="761360055C5148F1" modified="2017-01-18" version="1">MSDSEEPKVEATANIVSHDAGTETASTGTAAEVPTNLNEQGSHESSEERTTEAAEPAENTTATEQQQAAQPPSSTNDETHAPQTNEAARTTKESGRAAVQDEDEDDDDEEEDDEDDEDDEDDEERRPAKKQRRERNRFLDIEAEVSDDDEDEDEDEDELIREGFIAPSGAGAEGEDDEDGARSDRLHRKLDADRDRDQEEDAQRLAKELKERYGRTAASQASVVHDGFIPQRFMLPSVDNATVWGVRCRPGKEKELVKKLFKKKITLDKASDSKKLKILSIFQRDNYSGRIYIEAPKQTVIEKFVRGVPDIYFTQKLLVPVQELPSLLKATKSSDVAIEEGSYVRVKRGMYKGDIAIVDQISDNKLEVLLKIVPRLDYGKDDEYDEEGNRIRRKRKTFAERPQPALFNPTLAYRLDSSNIQMKGTSQYSYKNEDYIDGYLYKSFKIQFLETNDIQPSVEELTRLGGKDGQIELSNISLNMKKAQAANAVFQPGDRVEILSGEQKGSKGIVRSLTSPTIVSIKLPQFPKLLDYPSSSLRKIFEAGDHVSVVSGEHAGDSGLVLKISNGQVTFVSNQTKQNLTITANNLSKSIDSTPTSDDFVLHDMITLGSKEIACVIQAGHDIFKVMEADGKITNVGKKSILGKIDTSRSRVTTIDSNGNEIKIGDGILEKFGARREGRVLFIQQHHIFVFSRNISDNSGVFVVSPMTVEAISSRGQDAGLSQLQQQQQQQQLPQTQQQPGSMNLSRMNPEVFNKMGPPGSKTSSLQEKQQIRFGRDVAQGKTVKVRTPGYKGQLGIIKDVTGDNATVELHSSKKSIIIDKRKCIYFNNEGGKGISYDELVSRKGFVPNSNSRNNYANNNSNGGDRRSYVSSSARLPSAPMAMQQQQQQALGSATPGWGAFAGGKTPSVANAGMATSYGGASTWGSSGGFGGASTWGGNGGNATAYGNTSTWGGGGNNTAYGNGTAYGGSSTWGGGNNTTHGGASTWAAGNGTEYNSNSTGGNNGGGASTWGGASTWGNTTQTHNNGNTSTWGQPDQYQQNQNQGHRSTWGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVI3</accession>
    <name>A0A1E5RVI3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved oligomeric Golgi complex subunit 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g553</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2790">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="108"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="260" mass="29948" checksum="CF6924F6A057D199" modified="2017-01-18" version="1">MTENHNGTDDDMIEMFFDADFEPQTYLNMLLSSSSVSRLDREGESQQSLSTIQTLKSLQPITNALIKKFDFYTTNFTKEYERNVSTLEDPINDIIVSGSKKTKIEFYLETLNSSISSLGSEVQKLEAQIAQNNGDLAKDKQXDFLGDLKSLETIKTRLLNVLDCFIKINEAINXEDQHQVSVQDFKENLLSYQXQLTGDLRSLPTWSENESAEFIKQVRHLIELKHLFRGFDLFYPVYMEFSQALKKEVNDYLVRSGTQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVM2</accession>
    <name>A0A1E5RVM2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">rRNA-processing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g539</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Involved in nucleolar integrity and required for processing of the pre-rRNA for the 60S ribosome subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Nucleus</location>
            <location evidence="2 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the CGR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVM2"/>
    <dbReference type="FunCoup" id="A0A1E5RVM2">
        <property type="interactions" value="164"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVM2"/>
    <dbReference type="InParanoid" id="A0A1E5RVM2"/>
    <dbReference type="OrthoDB" id="3942380at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005579">
        <property type="entry name" value="Cgr1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03879">
        <property type="entry name" value="Cgr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 8" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="54"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ90876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="127" mass="15301" checksum="FB77CED307FA2D50" modified="2017-01-18" version="1">MSAKEQIEGAADNDQAALAQGNNKSGRTWKNTSKPLKPHSQVIKNKKLSSWELKKQQRLEDQQFKQKLKELKQEKEDEKQAHVQRIKERREMREEKERYERLALKMHAKKVERLKRREKRNKALKER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVN4</accession>
    <name>A0A1E5RVN4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g532</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVN4"/>
    <dbReference type="InParanoid" id="A0A1E5RVN4"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000433">
        <property type="term" value="P:carbon catabolite repression of transcription from RNA polymerase II promoter by glucose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001297">
        <property type="entry name" value="Zinc finger and SCAN domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051007">
        <property type="entry name" value="creA/MIG_C2H2-ZnF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47428">
        <property type="entry name" value="REGULATORY PROTEIN MIG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47428:SF1">
        <property type="entry name" value="REGULATORY PROTEIN MIG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="40"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="53"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="190"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="276"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="399"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="474"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="625"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="81"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="114"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="190"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="308"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="321"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="407"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="474"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="626"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="643"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="656"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="674"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ90885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="714" mass="77239" checksum="C3957BCE7F894758" modified="2017-01-18" version="1">MAYPKDDESRPHVCQICSKAFKRNEHLTRHLNTHTGLKPYECTYPECGKKFSRSDELKRHQKVHSNTLPRAKRGRKKKSEISANESNKSETNASGTSSVNANPDLPHNNTSTFHNNQSNHSSGHSSNVNIYKHINNSQSSSSNTNSGNTTSEESNASLMSVGYSNNNNNNNININSFNTGTGYYNNGMNNYNNAYNNNNNNNNNNNNNNNNNNNNNNNNNNNNNSNIPQHAIFTQNNVSVPHHNSLPGLSVALGGSNSNNTGYNTMSTGHIHNMMSTGXSTYGNNNSTAHTKSPVSFQLGDDREDAQSGDNHLSNNIADTNSYNSNSNNSYNNNNLNVGQPFVRQSRSHGNSIGSVNHMEHMAYSNRNNNVSNNSQPTSPGHALKLNALSTLKLMTPLNQPAKQSPNMGSNSYTSTNNSSTSISNNFNPLSRTGSNNSLNNMIYPSNTEFTTFSRASSTNSLSNLASRSGSLTNLSVLNSNNNSNNNNNNNPNNSGKYLDTGNYKIHKSSNSVTSLTSLSNGFVGSTSRKSLPTTPKRRNSNAFLENLAMTSTTNNTSGTKNDGFENTLTNKLQDLINXRSESPMPLKTXASTVSYAPATNQDQHLVASGTQTPLLVYVPTPTNATTTKSSLTPSTTFASPVYPQTNATSSSSMSITPNNMGNNTGNANINMESAATNQNTSGNNQNRQDANGTESTLPPIRTLNIDFPTDGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RW29</accession>
    <name>A0A1E5RW29_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ribosomal RNA-processing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g429</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RW29">
        <property type="interactions" value="452"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RW29"/>
    <dbReference type="InParanoid" id="A0A1E5RW29"/>
    <dbReference type="OrthoDB" id="2019504at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010301">
        <property type="entry name" value="RRP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13026">
        <property type="entry name" value="NNP-1 PROTEIN NOVEL NUCLEAR PROTEIN 1 NOP52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13026:SF0">
        <property type="entry name" value="RIBOSOMAL RNA PROCESSING 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05997">
        <property type="entry name" value="Nop52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="257"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="267"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ91039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="283" mass="33423" checksum="1C713A6BBA2569A3" modified="2017-01-18" version="1">MENSAFVKTLASNNRSTRQGALDSLNNYLQKKDLKQQDYDKLWKGLYYSMWFSDRPRPQQRLANELGELFVNDVFVKSSGNAVINTKSKLPYNQENFIKFAKSFWKVILLEWMKIDHHRLDKYLLLIRRVFYNQILYLHKQEYDQLLVQNYLTRVMERFPLSGDVRVYNGIPLHLIDIFVTEWERVFQTXIDDADDADDVEIDAAVVKNSCVPQFIELFVKLTKDVDVSKVLKTKIKEDILHDSTLLQWDVLXKDAPSAEKDASENQNDEEEEEEEEEEWNGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RWC1</accession>
    <name>A0A1E5RWC1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RNA exonuclease 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g358</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">3' to 5' exoribonuclease required for proper 3' end maturation of MRP RNA and of the U5L snRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the REXO1/REXO3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RWC1"/>
    <dbReference type="FunCoup" id="A0A1E5RWC1">
        <property type="interactions" value="97"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RWC1"/>
    <dbReference type="InParanoid" id="A0A1E5RWC1"/>
    <dbReference type="OrthoDB" id="3996471at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06145">
        <property type="entry name" value="REX1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000031">
        <property type="entry name" value="RNA exonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034922">
        <property type="entry name" value="REX1-like_exo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF118">
        <property type="entry name" value="RNA EXONUCLEASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 13" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exonuclease" evidence="12">
        <location>
            <begin position="233"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ91013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="387" mass="43848" checksum="5EDCAA25814AFAB1" modified="2017-01-18" version="1">MVGAIKPRDLPSFHAVGFQDRVKFVKQLYESVSKFYKNNPLTQQKLVRKCQFLEYKIAKLSKTSQNYKFKLGLLLRDIQKHKGNFRKFEANIFPTEDKSSSTLPKLDSKDPKVYERLLNLVSTEKVLLESGYITSVPVKSELLMDPKTASLSKYADCARCTLKFDKTDIMKDAVCKYHKDKGNFQSGVRVYSCCGVQEGCSLSNHHVFKGATPLDLLEISDFVNTQEVDGEYNVLALDCEMGYTSLGFELIRLTIVEFFSENIVYDKIVQPIGEIIDLNTDFSGVSKIDCILDPTFVNAREEYITPKLINKNSLLIGHGLENDLNVMRLVHDKVIDTAILFAKDLKYKPSLKNLTFLELSKRIQVGEHDSSEDAIAALQIVKKKIAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZG2</accession>
    <name>A0A1E5RZG2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">V-type proton ATPase proteolipid subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g183</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Proton-conducting pore forming of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Proton-conducting pore forming subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10 11">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d, e, f and VOA1). The decameric c-ring forms the proton-conducting pore, and is composed of eight proteolipid subunits c, one subunit c' and one subunit c''.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Vacuole membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZG2"/>
    <dbReference type="FunCoup" id="A0A1E5RZG2">
        <property type="interactions" value="364"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZG2"/>
    <dbReference type="InParanoid" id="A0A1E5RZG2"/>
    <dbReference type="OrthoDB" id="1744869at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18175">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18176">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.610:FF:000001">
        <property type="entry name" value="V-type proton ATPase proteolipid subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000245">
        <property type="entry name" value="ATPase_proteolipid_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011555">
        <property type="entry name" value="ATPase_proteolipid_su_C_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01100">
        <property type="entry name" value="V_ATP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10263">
        <property type="entry name" value="V-TYPE PROTON ATPASE PROTEOLIPID SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00122">
        <property type="entry name" value="VACATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <keyword evidence="11" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="122"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="12">
        <location>
            <begin position="12"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="12">
        <location>
            <begin position="88"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ92347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="157" mass="16115" checksum="A0A0210B62B43CD3" modified="2017-01-18" version="1">MFDVYAPFFGAIGCAAAIIFTSFGAAYGTAKSGVGICATCVLRPDLLFKNIVPVIMAGIIAIYGLVVSVLVCYSLGQKQALYTGFIQLGAGLSVGLSGLAAGFAIGIVGDAGVRGTSQQPRLFVGMILILIFAEVLGLYGLIVALLLNSRATQDVIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZL3</accession>
    <name>A0A1E5RZL3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptide hydrolase</fullName>
            <ecNumber evidence="3">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g141</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZL3"/>
    <dbReference type="FunCoup" id="A0A1E5RZL3">
        <property type="interactions" value="154"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RZL3"/>
    <dbReference type="OrthoDB" id="3907302at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016603">
        <property type="term" value="F:glutaminyl-peptide cyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040234">
        <property type="entry name" value="QC/QCL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12283">
        <property type="entry name" value="GLUTAMINYL-PEPTIDE CYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12283:SF6">
        <property type="entry name" value="GLUTAMINYL-PEPTIDE CYCLOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009028126" description="Peptide hydrolase" evidence="3">
        <location>
            <begin position="19"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="4">
        <location>
            <begin position="114"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ92391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="345" mass="39472" checksum="C48CB73F98228520" modified="2017-01-18" version="1">MLTATILWLAVFAFVCNGRVINDFPKIDTFLSLANPTDSLLLPFNKTRIPGSLGSFEVQTHIKDFFASLPVSWTLEEDRFAEQNHNFTNLIFSLNTGSLSAFSESEIEQERAVGTNESFFVIAAHYDSKKQPEGFIGAIDSIASCSIMMYLAFHLSDQIQQSPLAPLNRGIKFVFFDGEEALEEWSETDSIYGSRHLAKKWHAEDLLGRIDAFILMDLLGGVKQGLRDVKVFPFFEQTVGFYNQLAEIEQRVNKLDSLKTRYLEPEVLAFLNYNKQDPDYLPVEDDHIPFYKLGVPILHLIPYPFPAYWHTTEDDFEHLDISGIHKWAKMLSTFLQENLFEDEGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S026</accession>
    <name>A0A1E5S026_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">asparagine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Asparaginyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g151</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Asn) + L-asparagine + ATP = L-asparaginyl-tRNA(Asn) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11180"/>
            <dbReference type="Rhea" id="RHEA-COMP:9659"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.22" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5S026"/>
    <dbReference type="FunCoup" id="A0A1E5S026">
        <property type="interactions" value="927"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S026"/>
    <dbReference type="InParanoid" id="A0A1E5S026"/>
    <dbReference type="OrthoDB" id="1931232at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006421">
        <property type="term" value="P:asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04323">
        <property type="entry name" value="AsnRS_cyto_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000040">
        <property type="entry name" value="Asparagine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1910.20">
        <property type="entry name" value="asparaginyl-tRNA synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004522">
        <property type="entry name" value="Asn-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048952">
        <property type="entry name" value="AsnRS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00457">
        <property type="entry name" value="asnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF16">
        <property type="entry name" value="ASPARAGINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20917">
        <property type="entry name" value="AsnRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="13">
        <location>
            <begin position="253"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="70"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ92409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="556" mass="62582" checksum="CFDAD189ED0E8765" modified="2017-01-18" version="1">MSELYINEYTGKDATTQTGTKELPFQTAAYALFTIASADSAEPKLFVFKKTEQEDKSIVEEYVLISASALKKAKKGAEGLKKKAIKQQQQQQQANVQEITKKIDILSFNDLKLDESLPEAVPAKIYQSYDLVDKRIKVSGWVHRLRVSKKYAFLVLRDGTGYLQAILSGDCANAAQALPLESSIELYGTITKLPEGKSAPGGVELVADYYKVVGLAPSGDDAFTNKVQENADPSILLDQRHLALRGETLSAVMKVRAAFLKAVRRFYEEERLTEVTPPSMVQTQVEGGSTLFKLDYYGEEAYLTQSSQLYLETFLAALGDVYCIAESFRAEKSHTRRHLSEYTHIEAELAFLDFKTFLQHIENLLVKSVKYVLEDPIAGPLVEQLNPGFKAPEAPFMRLEYKDAIKWLQEHDIKNEDGENFKFGDDIAEAAERKMVDTIGVPIFLIRFPVEIKSFYMERCKDDPRVTESVDVLMPTVGEITGGSMRIKDTDALLEGFKREGIDPAPYYWFIDQRKYGTCPHGGYGLGTERILAWLCNRFTVRECVSYPRFTGRATP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0A3</accession>
    <name>A0A1E5R0A3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4576</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0A3"/>
    <dbReference type="InParanoid" id="A0A1E5R0A3"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="16"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="168"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="666"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="943"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="975"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1143"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1277"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="781"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="38"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="184"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="224"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="274"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="681"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="750"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1024"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1194"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1286"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1320"/>
            <end position="1330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1330" mass="144390" checksum="6ECC92DE043C4750" modified="2017-01-18" version="1">MKPTLAKGGHLDYLFDKLDPKTKEEQERRQKLEDEQKTYLSKYNKNSKTTFRSAQDQRKAQVLKKEKKFPQYGGSTLLNKSDGPVLDIGLPKKLPKREDKQKNALGKIDQKLNVLEGNKAKLEKNIENYNRIYGTGDLPSKNESKNGSKNVQNEQHWYSNVFGSNKKKLNTFTPQVPKPIYLPDTEKTDTEKPDTEKTANGPDSLEEFTKPETVLNEEPRADVLEDEDDSENVEFFDSNTDGAEPVEEDEVQKSTSEVSELAVAQGITKPLEQPQESTVVPVNDENLTVEPTENTTETDTGAVETPEVPKQSTVVPVNDENITVEPIANTTETDTGAVETPEVPKQSTVVPVNDENITVEPIANTTETDTGAVETPEVPKQSTVVPVNDENITVEPIANTTETDTGAVETPEVPKQSTVVPVNDENITVEPIANTTETDTGAVETPEVPKQSTVVPVKKTIAAQPTAIAVPGQSTKDSNGTQKKSPKLYDDTFYITKEDSKAIKQQYDTKISSLDKQLQQFPSQYTKGLENTDYKTLMAKYSAMDALQKELNTLLGKQIELDALLKVGKNKKSLELQRLNQLNLKKHKLDKLHKRQRHLHREKKRALINAKKSKRNDFNEKIRSENAKIKQENEKITTNNNETLKSNADIKKKNAHFQALKRERAEKYPETHGHYAAPSLTPATTRATHTTTHTVHTTGSTTTNSDSIVPSDLHPAADATTSVISEPTKPVEKETNPLAPVSSASHAEEPETENVVADDDDDDDATAEIASEATSYIPGEISENEEDLADIEDNEEQIDEEFQNVSQDPLITADEKKQYNDQKFNLESYRLDINKKLDQFNTLQQEITAILTKPTTRTTLQTTPKMVAQPSATPSATREVSTAAGFEPAYQLKDLKKLGNQINAPSDDELTKLSDLAESSEVDETIAPKETLIAPIAVVDPIALESEPEPSPIPTREPTPEPEVAAAAALVPVGSAGPVVPAGSTEGGTESAVPATTNKSVEPTDPEETAGALVPTEEAGPVATTETTEPVSPVATNKSTEPALAGAVGAVVPTEPAGPVVPAGESETPEATEKAEPTNKTLGGIAVVAPVIPLDNVVPAAPASAGQSGDAGVPLNSVVPEVSRAPAQDKAVEPAVLSEVKPADSDLPVEKPTQILPPVENNQNGTFEHAEKPTAVAPPVEDEKDTLANPVENETNKESKPRKSLVEQMKDIKPEVVEEEVTTVQPITLAPGEEPPAPTYHSKIEEEVKTPTSSTFTQTTIKSPAEFKDLPSTILADGKQDKYVSSPLNKNVAGTSFTKNKNNSESSLFSEYSEEEIVSAPNTETHSLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0L5</accession>
    <name>A0A1E5R0L5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4463</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R0L5"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="117"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="359" mass="40430" checksum="838C99A529B752E2" modified="2017-01-18" version="1">MLKHTISYLRTPSIKATTLVRCVHNANKPGSASPDDFIASLLKRMDSVENSPKLPKDFDSTVDLSALDKIARPSKKAPRIAKQTADLSFDVSDLDEGNVFTKTSTPPRKSXTKSRFDNNSNNNRGQNRPPRRDRPGMRNARANGKFTKKPDGKPKNVKVNITEESFENKRTIKMQSNEFISTTPYQLEHLSLLSLHRDQSRLNNSSNDSSIAXSYAIKIASNSNLPIDAFLPNKPIYNDQGKPVNTSEIVVKPLRNGVTVGHYTEPIVLQHTPFDKNFKFKHDKDLLKAMIHGQYPTLNALDKGMFVKKFXEKSDFFQRNSESIRKSLQNSYLNTADLAKKEQMLRVCSGLDPVSSLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0L7</accession>
    <name>A0A1E5R0L7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Structural maintenance of chromosomes protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g4549</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMC family. SMC2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0L7">
        <property type="interactions" value="1086"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0L7"/>
    <dbReference type="InParanoid" id="A0A1E5R0L7"/>
    <dbReference type="OrthoDB" id="10255539at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03273">
        <property type="entry name" value="ABC_SMC2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000278">
        <property type="entry name" value="Structural maintenance of chromosomes 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000385">
        <property type="entry name" value="Structural maintenance of chromosomes 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027120">
        <property type="entry name" value="Smc2_ABC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43977">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="14">
        <location>
            <begin position="535"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="283"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="423"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="701"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="765"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="292"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="15">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ80442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1189" mass="135178" checksum="E8171583F39939ED" modified="2017-01-18" version="1">MKVEELIVDGFKSYATRTVISHWDPQFNAITGLNGSGKSNILDAICFVLGIASMATVRASNLQDLIYKRGQAGVTKASVTIVFDNSDRSKSPIGFETYPKISVTRQVNLGGTSKYLINGHRAQQQTVLQLFQSVQLNINNPNFLIMQGKITKVLNMKPKEILSLIEEAAGTKMFEDRRQKAEKVMAKKDIKLEEIKTLLKEEIIPKLDKYRNEKRLYLEFQDIQLQLEEIENVISTFDYHNLSMQFNDSKQVSDDQTAKMTELQXEKTSLAESLDILKQELEKANSKKQNSEHVNANSNGNSQHGKDIKTLEKVEAELSNEISRLNSSLNLQKDDLKDDQAKLTDLQKKMGNMLDQLHSLDESCRIKTEEYESIRKRFETMQSDHRLKEELLSSLTMSLSKSGGESVSAGGYQQQIAALRGKLNQVNITIKSTEMKVNALENERLRNNQHLEQARAGHENLQNKLESLESEKLISVKKLQSFGYDPNVLKELKKKEIHLKNNVHNFKNQAEQWRRKLYSLDFQYAKENLKLASKDAIKGVAATLFTLDESKHDMATALQVCAGGRLYNVVVDNEKTASQLLERGRLKKRVTIIPLNKISTRTIRNETVLFAKSLSPENTGDVDLALNLIEYSADVTKAMEFIFGTSLICKNADLAKKVTFHPKIRTKSITMDGDVYDPEGTLSGGSRNNNGVNILIEVQKLNQCVQQLRLLEQELEDVQRLIKKHQETSEQTRDLQESVNLCEHKILTLTKELENSLSNQYGARNAEIEKTSKNLQKQIETSKLEISNIAKNISQTESDMKEFNSNKGAKLEQLKSQVETLSKDVEVAEDSHETAYDEQQTVLMEREQVVDEIEELKRQEQETLSNIDVLQNAISSTKEQAVLKSKEFGNVHLILTQEKENIRQINEEIKQLSNTIKEKSNALKQTEVDYQILLNEHQNFIQNVGSLDSRLKVLLEKHSWLDDEKMVSGIINRNSNIDLNEFKSRREILATKFSTMKSKVNTNIMSMIENVEKKESTLKTMIKTIEKDKKKIGETVKKLNEYKKDALVKTWEKVSVDFGQIFSELLPNSFAKLVILEDKEITEGLEVKVKLGKIWKESLVELSGGQRSLIALSLILALLQFKPAPMYILDEVDAALDLSHTQNIGHLIKTRFKGSQFIVVSLKEGMFTNANRVFRTRFQDGTSVVNIXT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0N6</accession>
    <name>A0A1E5R0N6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">GPI ethanolamine phosphate transferase 1</fullName>
            <ecNumber evidence="13">2.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g4399</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the first alpha-1,4-linked mannose of the glycosylphosphatidylinositol precursor of GPI-anchor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Endoplasmic reticulum membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the PIGG/PIGN/PIGO family. PIGN subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.-.-.-" evidence="13"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0N6"/>
    <dbReference type="FunCoup" id="A0A1E5R0N6">
        <property type="interactions" value="478"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0N6"/>
    <dbReference type="InParanoid" id="A0A1E5R0N6"/>
    <dbReference type="OrthoDB" id="2748310at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051377">
        <property type="term" value="F:mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16020">
        <property type="entry name" value="GPI_EPT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000015">
        <property type="entry name" value="GPI ethanolamine phosphate transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007070">
        <property type="entry name" value="GPI_EtnP_transferase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017852">
        <property type="entry name" value="GPI_EtnP_transferase_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037671">
        <property type="entry name" value="PIGN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12250:SF0">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12250">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN, CLASS N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04987">
        <property type="entry name" value="PigN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 13" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="466"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="509"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="535"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="568"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="594"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="622"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="646"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="671"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="808"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="852"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="883"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="917"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfatase N-terminal" evidence="15">
        <location>
            <begin position="217"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="GPI ethanolamine phosphate transferase 1 C-terminal" evidence="16">
        <location>
            <begin position="450"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ80450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="959" mass="110187" checksum="FE661542E43B51B6" modified="2017-01-18" version="1">MWSTQRIVFITVGVLFHLFYLWSIFDIYFVSPLVHGMKHFPSTTNPPAKRLFLIVGDGLRADTTFDQVVHPLSKTRTPEYLAPFIRSMALENGTWGISHTRMPTESRPGHVAMIAGFYEDVSAVTKGWKENPVDFDSVFNQSKHTYSFGSPDILPMFKDGAQDEDKIDTWTYGHEFEDFTQSSIELDAYVFRHLYELMDNSTTDKFLHDQIHQEGNVFFLHLLGCDTAGHSYRPYSAEYYDNVKYIDAQVKELTRKVEDFFQDQDSAFVFTADHGMSAFGSHGDGHPNNTRTPLVAWGPGINKPKTTEERASKICIDSEQEDLEYTKNWALGHLSRVDVNQADITPLMSYLVGSSYPSNSVGELPLAFINASSSEKLHALYNNAKTILEQYLVKEQEVIASQFTYKPFHRFQEKSSVERLKEIEKLIDSASVPDAIVIEKIEELMKLTLEGLQYLTTYNWRFTRTIVTFGFVGWITYSFMIFVKSFILTSSELNSFSTANKDQNNRRSTLLMVVFIVIGALLNYVLFYQRSPFNFYMYLLFPLFFWNRILTNFGSLQRGTKKFFQGLPLWKILVIFLQVIFIFEGIVYGFFHRWIFTAIFNVLAFYPFVYGSRQHWKYKMSWIVTSICLSIFTLFDAVKIESLTQINLAGLLIVVSAVWGLIKVRAKINNFTLNVFIAQLVLTVLMILVTNKSVSSLQGRNGLPKDAQVLGWSIFVCSLVAMPLCHTKTKSAAINDYQVRMLIVYLTFAPTFIILTISFESFFYFFFSIYILQWIEIETNIKFAQITQQSMEEDQKENTKIDSNYLQILRISVIGFFLLQVAFFGTGNVASISSFSMDSVYRLIPIFDPFPMGALLMLKLIIPYVLLSTGLGILNLKLHIHDYTISSLIISISDILSLNFFYLLRTDGSWLDIGVTISNYCLAILSSLFMIILEIISHILLRNVYIEKKDTLLQMKKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0U5</accession>
    <name>A0A1E5R0U5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4582</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0U5"/>
    <dbReference type="InParanoid" id="A0A1E5R0U5"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="127" mass="14279" checksum="77C22BA0A2EA4253" modified="2017-01-18" version="1">MMLSFHNPATLLNKACPVIKKTLHNNSNTQSNNTNTNTNTTEKRMLPCIRRTNTNTSTSSTNSISSSYSTTSTNNKNSTKHSSRRSSKSRMSNPDPVFKQKVITKADLIKYNQKKYNINIEFTDTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0Y8</accession>
    <name>A0A1E5R0Y8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g4595</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0Y8">
        <property type="interactions" value="556"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0Y8"/>
    <dbReference type="InParanoid" id="A0A1E5R0Y8"/>
    <dbReference type="OrthoDB" id="1108038at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16702">
        <property type="entry name" value="RING_CH-C4HC3_MARCH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13145:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCHF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13145">
        <property type="entry name" value="SSM4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="402"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="468"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="500"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="542"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="599"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="666"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="709"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="899"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="954"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1035"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1094"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1141"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1194"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="15">
        <location>
            <begin position="12"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="329"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="334"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ80547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1251" mass="145051" checksum="16F02F00BF2A1D16" modified="2017-01-18" version="1">MSSSLYSAASADTPIEEHTCRICRGEATEEEPLYHPCQCKGSIRYVHEKCLMEWVDAKLNAGNRGNGGNNQDNDDDDNDNNVLQMDLTKDLNVKCDICHHPLKFKTIYDENTPDSLGFFTIITNFFQFLYQKTLKSLLMIAFALMFLVFPMVWGIHARFYTFVLDQKLPYPGQLWKSMIYGYGDDSTDVIWQIFHNNNLFFTLQMIFVVIVNLAIYFLYDMVAREKVFLQMLQFRRGPQFTMKELLIKQFKRTLPGLDDSSLNRLADMYMSNQDNTNGAPTQVQEAVPLADHNHENANDVIEEEEEEEEIDQPDRAAMDEFDDLIQVREREQQQQEQQQEQQQEQQQEGREEQVDADGQVNEAAPNHEQQQAPPIPVQVNAVQQDREAGAFILNLTLSWVTIVKYLAIGMLAFFAYQFISYFLPTLIGYGLIRKYAFLIKYLFKMTVFLMHIFKIDKGVSKLSGYDQFFMPTIANLYFSILNSVQNIYYNYTTNAFPGSVWYRTMPAATTFMTFIMLTCVAAEYMHRGYGPNNGMKNPTQRLVFYVLFAIEYTSKVFFIFVTELAGFPILAGIMIDWSLLSPCLLPQTENFIVPKLTNFLSVPVSFIVYWCMGTLYMYWFSRYIGMIRQYILRSGVLFFIRSPEDPNTRILHDSLIHPLDVQISRLCLSMFIYSVIILCGFGFFTRLAIPEFFPSLLPMGPNLDYVNDSWFLYKCLALIILFYLTKTIVEMHTPTLKVVIRNYWSASFKLICAKLRLSSFILGIDSVEERGYIHYRNWWYKYIHSDKAQHSNMNLFTNPQSLKMANNLFKENPLVHAYFIPDGSLMRVPGSDIVSRNFIKTLFVQVTKEDKLIKKNDFEKAQKKHKVGAFDHLDIQSTEFDEYIIVYTPPNFNVRYHALLVLVWLFSSFLFIGSAVVANYISKTLFILLGYPVSLVFKKTEVIFFKGYFKTVSVXHTCFGLMILASLIQSVFIRMERLKKLRKEQEAAANAAGVSVGGNGDERVVEPLIQVAGDFFADGNFVGIRQIMNNFITNVFFYHFLMFGIRCLSLILFISSTFFQIFTIYDFTVYKFFGLGPSMKEAADQMSDYLDNSILVRVGSLIASWILFYGVYKGEPNANEGRSWKNVYALITKKFRIFLKFCLLPIGGKIIIWALIVLIEYFANPFFSTTSVWAVYRDYFNGINRKDYLFDAKMYKVFCIAPLVVTFVFLIPVLKTWYKDFINHANDIVKRKIYARGRTLENLNTEEDETN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0Z9</accession>
    <name>A0A1E5R0Z9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">N-alpha-acetyltransferase, 35 NatC auxiliary subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4653</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAK10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000017">
        <property type="protein sequence ID" value="OEJ80243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0Z9">
        <property type="interactions" value="637"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0Z9"/>
    <dbReference type="InParanoid" id="A0A1E5R0Z9"/>
    <dbReference type="OrthoDB" id="269405at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031417">
        <property type="term" value="C:NatC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057983">
        <property type="entry name" value="NAA35-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007244">
        <property type="entry name" value="Naa35_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057982">
        <property type="entry name" value="TPR_NAA35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21373">
        <property type="entry name" value="GLUCOSE REPRESSIBLE PROTEIN MAK10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21373:SF0">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 35, NATC AUXILIARY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04112">
        <property type="entry name" value="Mak10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25789">
        <property type="entry name" value="TPR_NAA35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NAA35-like N-terminal" evidence="5">
        <location>
            <begin position="75"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="NAA35-like TPR repeats" evidence="6">
        <location>
            <begin position="376"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="37"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="7">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="804" mass="92711" checksum="0217D7073F8E0B47" modified="2017-01-18" version="1">MSENHELQPKESEKGHHHYQEAFEKLHISLESELSEEQERLPESVEISKEQPQHANLTDVTELFLRLKSKLSPAKVVKAEQFNLFAGTYALEINNSKLDTGLIPEDFPKFETTCLLHEVYTSYSAIQHVNNILNHLIKYLVTWMDDETSNSLSTTLLSCKYVQFLLEEYTKTLQVPSTFSTGTAVYDQLLRCGIVGILYFVKFIKSKVNPGFVFEEEDMHLSMLNLNCLSEIPNPCEIVSDLKDLKAQYCLGQSDIDLAKLEHTLDIIIGICEIEHLLDNGSSCEAEETFSKLINSCEAMAVYDKDEYTLPPNLFSMQVQKTLSNFVPPKSLVPPIKAPYRKFISILGELKTVFTALFDLSNPYDINQFVYYYFTTNKQLNVITRILYPHVLCENGDVNFVNMQYVVDDLESVVLLNQDSKSMILDYMNNFLQYDVESLQETLGTYYNNFASNHCRYRQGYNRQLLMWDSLQARFSMHEETSSGSDASTSEENFPEVTSYVYYRKLKNMLEFQFRGFHLEIYAPWETFSIYWHCYDLLLNLDYLLNKMIHHNDSIIDRLERANLSKNLKKKYKLVEKRKAVKQKNQNLLQKAFRIRKNLSLEQNVAKIYSILSLVQLLQFARLYRXWNSEDKAKYEKFMKDQLLHDLRFKSFQSFGIPQTPELKTILGSLQMFVASQEKPGADLESSIKTQLETCKMAIKTVLEEMQTTQTNNAISEDAVSISVMPSRVNGTALDWFVQLQKTCDAFLESYGPASTSSSSSSSQSSFYPSKAAGFDNGKNNMQQKSSIQLNLNKDTHMFFPLYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1D4</accession>
    <name>A0A1E5R1D4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative ATP-dependent helicase IRC3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4100</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1D4">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1D4"/>
    <dbReference type="InParanoid" id="A0A1E5R1D4"/>
    <dbReference type="OrthoDB" id="16911at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036121">
        <property type="term" value="F:double-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061749">
        <property type="term" value="F:forked DNA-dependent helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000403">
        <property type="term" value="F:Y-form DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032042">
        <property type="term" value="P:mitochondrial DNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070125">
        <property type="term" value="P:mitochondrial translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18799">
        <property type="entry name" value="SF2_C_EcoAI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050742">
        <property type="entry name" value="Helicase_Restrict-Modif_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47396:SF1">
        <property type="entry name" value="ATP-DEPENDENT HELICASE IRC3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47396">
        <property type="entry name" value="TYPE I RESTRICTION ENZYME ECOKI R PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 5" id="KW-0347">Helicase</keyword>
    <keyword evidence="1 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="3">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="4">
        <location>
            <begin position="264"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="428"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ80687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="696" mass="79869" checksum="EC23037390184B90" modified="2017-01-18" version="1">MVHRRELAIQAEKTIKQFHPHLNVQIELGDSKTYMTLKGELSDLRMSHAGKXGAGPGTKIARKFNSNVQKINAKLQKVRGHPDVVICSVQSLIRRLGXYDPKEVMLIIIDEAHHAVAKSYLEILEYFNALXPPLVDKDEXSSIISSATPYEPPVIGFSATFERQDKKALGQVMEKIVYDKGILEMIEDEWLCDAKFSTVQIASLDLSQVATNPSPKTSRTKTKENNLLEDEYGDFKVPQLSKVMNTPEINEIIVKTYLARREKIMNENMGHISTILFAVDIEHVHCLHEMFQKHGISTAFVTSLSKQHERDQHIEDFKNNKIEVILNCGIFTEGTDMPNINCILLCRPTKSRTLLVQMVGRGLRLHKNKQHCHVMDFVSSTKTTGIVSVPTLVGINDWQEILGEGEIEDFPYGDMRKEELSIQDLQKIKEKREMLRKQEQERQIVERENFNATLKEKYEQYINETGALDLTFTTFDSFSDFCASQKANXDSVSPFXQEYSHFXDSLIPWVNHTHNRWAYKIPKTTTHLSITKNNDSERHYVLKVLTEIPQYVRNTATSMMPKHNSKILLKSYSLGEIMAKAAEYLTHCPSCYVVKRSKFTSPHKQLTISKFAQWRKXSATDKQXQWIKKIAVQCMERSRSSSKQPVQDNLPTEQQXDHYLRAKLTKGEAQDLIFACSLSPQYPLRKFLRNVSYKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1K9</accession>
    <name>A0A1E5R1K9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA topoisomerase (ATP-hydrolyzing)</fullName>
            <ecNumber evidence="4">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g4532</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14 15">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TOP6A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1K9">
        <property type="interactions" value="790"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1K9"/>
    <dbReference type="InParanoid" id="A0A1E5R1K9"/>
    <dbReference type="OrthoDB" id="5377392at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042138">
        <property type="term" value="P:meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000706">
        <property type="term" value="P:meiotic DNA double-strand break processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002815">
        <property type="entry name" value="Spo11/TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013049">
        <property type="entry name" value="Spo11/TopoVI_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036078">
        <property type="entry name" value="Spo11/TopoVI_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034136">
        <property type="entry name" value="TOPRIM_Topo6A/Spo11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848:SF0">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21180">
        <property type="entry name" value="TOP6A-Spo11_Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04406">
        <property type="entry name" value="TP6A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01550">
        <property type="entry name" value="TOP6AFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56726">
        <property type="entry name" value="DNA topoisomerase IV, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Spo11/DNA topoisomerase VI subunit A N-terminal" evidence="10">
        <location>
            <begin position="115"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Topoisomerase 6 subunit A/Spo11 TOPRIM" evidence="11">
        <location>
            <begin position="249"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ80453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="451" mass="52080" checksum="50DB5A14EC326FD4" modified="2017-01-18" version="1">MNCNLSELIENLDISSRTGRTKEMKELHDQISKETTRRIIXKTFESSMNFEKTTVSKEKLLLETAQQLLRSLQAGIHGQCNVVIQLPKKDMRKNCYKELQFPSTSGAEEQTVANFVIVLSLLYEIESNLRQNKISTVRDIYYKNVKLYNKHQTVCQKWIEVLCDGFDAKKFELNIVAAQKGLFYYSKTLTLLENIKKSDKSLVKVFTGSNLIPYCFEHSSESYSFSLRNNDFEIYQDEAQQKQGIEGVAILEKDAIYNDLVLNSAFIQNSNWMLITGKGNPDHLTQAFVKYLSSQQNITIKKFYIFVDSDPYGINIAYNYYNFIKRAAKSAKAGIQNRNDCVNIEFKGVKLFDLIDIVAKKNLAMEKSNLFLETHQKVNSSAFQLIPLTRRDIRMCQCFLNKIALLKATQKDMFLCSLKTELQRQLFFKSKGEINSVQDNDFAKWFESSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1T4</accession>
    <name>A0A1E5R1T4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ankyrin repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g4369</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1T4"/>
    <dbReference type="FunCoup" id="A0A1E5R1T4">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1T4"/>
    <dbReference type="InParanoid" id="A0A1E5R1T4"/>
    <dbReference type="OrthoDB" id="9995210at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="87"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="171" mass="18693" checksum="77DFA0453F048B0E" modified="2017-01-18" version="1">MSDTTEGASLNEQLLDCSRRNNLDLLQDIFTALDNDSDKIAALINNAKDPLGNTCLHLCCQYGSWDVLDQLLDQDGGVEIDPVNASDGDTPLHVAVKYALDEPDHGTFICENLIECGADPRIRNKHKLKPLDLVHGNELETLIDLLQSAEIAANAGPVEEPEDLVDDDDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1U5</accession>
    <name>A0A1E5R1U5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">U3 small nucleolar RNA-associated protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4167</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UTP6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1U5"/>
    <dbReference type="FunCoup" id="A0A1E5R1U5">
        <property type="interactions" value="335"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1U5"/>
    <dbReference type="InParanoid" id="A0A1E5R1U5"/>
    <dbReference type="OrthoDB" id="28112at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013949">
        <property type="entry name" value="Utp6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055347">
        <property type="entry name" value="UTP6_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23271">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN 66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23271:SF1">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23240">
        <property type="entry name" value="HAT_PRP39_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08640">
        <property type="entry name" value="U3_assoc_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 6 N-terminal" evidence="7">
        <location>
            <begin position="8"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="216"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="231"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="243"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="444" mass="52259" checksum="1965AC92D3D8FECD" modified="2017-01-18" version="1">MSKARYYLEQCIPEVDDLVTQGIFTKQEVGSIMRKRTDFEHRLCSRGSSINDYVRYVNYEGNVDKLRVKRIKRLLDGEKKKGHSISDYSIQRRIEYIFQRGCNKFPTDLKFWSIYLNYLKSKGNKVSYKRIHSVYNQLLRLHPTNVDIWISCAKFEYEIYANFKSCRTVFQNGLRFNSDSEKLWYEYIKFELNFITKLLSRRKVLKLINEREQEMDMQNQRIESKGHDGEMENGNDTDDEEQSGVTMPTTGDSMKDKLNELPDVDISMLGNDETNPALKGDIALTIYDIAMKTLLDSFLKKHRGYALESTAQEDQELVDSGKQHIIDLSLQYIKLFDKFTELNRIYLINHVVQYIYNNIQDENIEMCLVELTLDVRYMTVETFQDNLLQYSVKKYLSFVSKCKNTQYKKDVSLAYKDYLRTTFLDELSVNDPKRGVLSSIITTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R203</accession>
    <name>A0A1E5R203_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">serine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Seryl-tRNA synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Seryl-tRNA(Ser) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g4062</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R203"/>
    <dbReference type="FunCoup" id="A0A1E5R203">
        <property type="interactions" value="880"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R203"/>
    <dbReference type="InParanoid" id="A0A1E5R203"/>
    <dbReference type="OrthoDB" id="10264585at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="10">
        <location>
            <begin position="170"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="303"/>
            <end position="305"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="319"/>
            <end position="322"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="390"/>
            <end position="393"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="436"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for serine binding" evidence="8">
        <location>
            <position position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ80938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="477" mass="53770" checksum="645DFD39BCDBE94D" modified="2017-01-18" version="1">MFKRFYSSGLKKPVFDIKSVIGNINGYKQSIHSRQLLNHDELALGLENLPLLFQSWKDYHQQLAEIQVQRKAIEKQLTLGKKFEQNAAKNSTGQSIDKNKLKNLKLQSQNVTELINNAQDKMNDICDSLPNLVHHSCPAVAPVTTDWINPLHDVSKDNQQESYVADPRKDHISILTQKGLVDFSAAASVSGTSFYYLFDEAAQLEQALIQYAVQRARAHGFRFTLPPSIARMDVIHSCGFRPRDDVASGEENQIYHLSNTDKGLIATAEITLAGLNANKVFHESAKTRPSLPLKLCGLSKSFRKEAGARGKDTKGLYRVHEFSKVELFIWCRPEESETHLLSLKNFQMDMIKELGLTAKVMNMPANDLGAPAFQKYDIECWMPGRGSFGETSSTSNCTDFQSRRMNTRFISHVSDGHDAKPNKQNKVQTQFVHTLNGTAMAIPRIIMAIVENFYNPQTKMVAIPKPLQPFMNNKTHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R232</accession>
    <name>A0A1E5R232_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Regulatory protein MIG1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g4324</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R232">
        <property type="interactions" value="566"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R232"/>
    <dbReference type="InParanoid" id="A0A1E5R232"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000433">
        <property type="term" value="P:carbon catabolite repression of transcription from RNA polymerase II promoter by glucose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051007">
        <property type="entry name" value="creA/MIG_C2H2-ZnF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47428">
        <property type="entry name" value="REGULATORY PROTEIN MIG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47428:SF2">
        <property type="entry name" value="ZINC FINGER PROTEIN RSV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="66"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="304"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="338"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="310"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="339"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="379"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="397"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="12">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ80968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="522" mass="55941" checksum="8C27D0ABE12C7F64" modified="2017-01-18" version="1">MEQHTTVAQQVMVNTQQNAVADPPYRILSYPYAEGRKYLCTTCNKAFKRHEHLRRHLATHTGQKSHVCTYPSCLKRFSRSDELKRHYKIHFKVKTKKTRNNANANANANAPRVKKLVDNNKSSSANFLGSESNKVSKLPKQTSPFTPTFQATTSLTTNTNIYASXSSVGGQGMPTSIMTQPSLPNLVLNKNPIQLPMPMPMTQSSGXSSXLPSVSIDRINISATPLLPRMMPPPLSVPQIXGSLSNSLPPVXTSXPTSVANSVPHSTLVSPTVSSTNLRFGSSTSLANNLTTALSDYQKQEHRPFGFSTPLHTNSPIPSTSAITNXDTSVNTTVLNLNETSPESTTSPTPASTNSAPSPAATSVSSVNSQSFSRSSFSTLPNPSSFSSAGPLGKPTFPSTVNLDTQNRGFGFTHSKGSLPSLSTTFGSLPPTTNNASPIFAPLTNPNTSGMNTTNXIMNGIPSTSVGHRSVKFSFHDEEYDETTTPKTSNIPPIAPALSHGVEPQQSINNQLPSFKNILNGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R234</accession>
    <name>A0A1E5R234_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">60S ribosomal protein L27-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g4061</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R234"/>
    <dbReference type="FunCoup" id="A0A1E5R234">
        <property type="interactions" value="1163"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R234"/>
    <dbReference type="InParanoid" id="A0A1E5R234"/>
    <dbReference type="OrthoDB" id="2365484at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06090">
        <property type="entry name" value="KOW_RPL27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.770:FF:000001">
        <property type="entry name" value="60S ribosomal protein L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001141">
        <property type="entry name" value="Ribosomal_eL27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041991">
        <property type="entry name" value="Ribosomal_eL27_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038655">
        <property type="entry name" value="Ribosomal_eL27_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10497">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01777">
        <property type="entry name" value="Ribosomal_L27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="144" mass="16573" checksum="448D7ED156B590F2" modified="2017-01-18" version="1">MTHSASEITRHENTEFQTAVIVRGRYAGKKVVIVKPHDDGTKSHQFGHALVAGIERYPLKITKRMGAKKVAKRTKIKPFVKVINYNHLLPTRYTMDVESFKASVSTETFENPAQREEAKKAIKKAFEERHQAGKNQWFFTKLNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R255</accession>
    <name>A0A1E5R255_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g4003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R255"/>
    <dbReference type="InParanoid" id="A0A1E5R255"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ80991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="123" mass="14059" checksum="938FEF787BC6D8C6" modified="2017-01-18" version="1">MTVSSRNFDKQSVLVHRDLRHNGCTLVARGNDESKLDGFKTRLSDTSLPPAQSVDHRIPNFGFEESSTALHTSKSAVIRQKVQSYVIFDKTGTRNKESFEESMWNLVQFIEEKDSRYVFSQDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R282</accession>
    <name>A0A1E5R282_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="7">PPIase</shortName>
            <ecNumber evidence="7">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g4002</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the cyclophilin-type PPIase family. PPIase B subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R282">
        <property type="interactions" value="242"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R282"/>
    <dbReference type="InParanoid" id="A0A1E5R282"/>
    <dbReference type="OrthoDB" id="193499at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000019">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0697">Rotamase</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009028094" description="Peptidyl-prolyl cis-trans isomerase" evidence="7">
        <location>
            <begin position="22"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="9">
        <location>
            <begin position="37"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="228" mass="25421" checksum="B382ABEC9127140E" modified="2017-01-18" version="1">MYKQFSFVLVLFAVIYQAAFAKAATPAEDPEITHKVYFDMKHGDKDVGRIVFGLFGTVAPKTVENFYQLSISDDPKMGYINSTSHRIIKDFMIQMGDFTHGSGVGGKSIYGNTFEDESFELKHNKKGRLSMANRGXGTNGSQFFITVNDDLHWLNGKHVVFGQVIEGYDDVVDYLQNVDTTRNDKPKLPVVIVACGDYKDVEPATAEXAEKATPEAVAQDEDIKHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2L7</accession>
    <name>A0A1E5R2L7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">tRNA (guanine(37)-N1)-methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.228</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">M1G-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA [GM37] methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA methyltransferase 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TRM5</name>
        <name evidence="12" type="ORF">AWRI3579_g4024</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>guanosine(37) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36899"/>
            <dbReference type="Rhea" id="RHEA-COMP:10145"/>
            <dbReference type="Rhea" id="RHEA-COMP:10147"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.228"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Mitochondrion matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="10">Predominantly in the mitochondria and in the nucleus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRM5 / TYW2 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.228" evidence="10"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2L7"/>
    <dbReference type="FunCoup" id="A0A1E5R2L7">
        <property type="interactions" value="1125"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2L7"/>
    <dbReference type="InParanoid" id="A0A1E5R2L7"/>
    <dbReference type="OrthoDB" id="408788at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052906">
        <property type="term" value="F:tRNA (guanine(37)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070901">
        <property type="term" value="P:mitochondrial tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.110:FF:000001">
        <property type="entry name" value="tRNA (guanine(37)-N1)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.110">
        <property type="entry name" value="Met-10+ protein-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03152">
        <property type="entry name" value="TRM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056744">
        <property type="entry name" value="TRM5/TYW2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025792">
        <property type="entry name" value="tRNA_Gua_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF36">
        <property type="entry name" value="TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25133">
        <property type="entry name" value="TYW2_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 10 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 10 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="11">
        <location>
            <begin position="227"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="368"/>
            <end position="369"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="396"/>
            <end position="397"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="460"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ81139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="570" mass="65912" checksum="AD802DA266501C64" modified="2017-01-18" version="1">MRAIGTFTLRVADTWYTISWMFAKVSRKNRAKTSILYKGLSNITGIYHFPAKSFSTTMKQQALKKFHFDLPSNRNYGLIKVDNSGSVNSAKNNNDFYTFDKNQFSVKIPLVAVIFPDPKNISYFCKNYRQDVFNCERLTNIVKLCTNKEDSEKKQILKGVLLKEEIDDVSKAEALLSPETYKFLQENNCTFKPYEYHLDYDFWKVEEVLCKLLPPGLNSAESIPSSYTSTGHIAHVNLRSEYKPYGEVIGQVILDKNQPKVQLVVDKLDSINSKFRTFDMKILACTADMDKQISNDLNSIFEVTHKESNCFFKFDFSKVYWNSRLHTEHERLIEQAFQKGELVGDVFAGVGPFSVPAGKKQVFVMSNDLNPYSFKYMQENITVNKVSDFVKPLNLDGVEFIQNCIPILNDYRKSHTDARIKILKKIPNNKKKDTNNAGKKANVVEELIDIPKFYHHFVMNLPDTAITFLHNFIGIYTKHFDPDNLPENFQLPYVHVHCFEKYLPEEAATITDHEVHSRIHQRVLQELDLINDKQHVLPLDKLKFHLVRKVAPTKPMYCVSFQLPQKVVFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2M4</accession>
    <name>A0A1E5R2M4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Exodeoxyribonuclease 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g4118</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2M4">
        <property type="interactions" value="189"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2M4"/>
    <dbReference type="InParanoid" id="A0A1E5R2M4"/>
    <dbReference type="OrthoDB" id="26491at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09908">
        <property type="entry name" value="H3TH_EXO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09857">
        <property type="entry name" value="PIN_EXO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000011">
        <property type="entry name" value="exonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000002">
        <property type="entry name" value="Exonuclease 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037315">
        <property type="entry name" value="EXO1_H3TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044752">
        <property type="entry name" value="PIN-like_EXO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF65">
        <property type="entry name" value="DNA DAMAGE-INDUCIBLE PROTEIN DIN7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="8" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="17">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="14">
        <location>
            <begin position="97"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="16">
        <location>
            <begin position="139"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="528"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ81134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="858" mass="97187" checksum="8382BDE64C4B67CA" modified="2017-01-18" version="1">MGVTGLLPQLKPIQNPVSLHRYEGCTLAVDGYAWLHRAAHGCCEQLCLGEPTTRYLSFLIKKIDLLKRQHKITPFLVFDGDRLDAKAGTELKRRVKREENRKLGCELHKRGEKKKAWEYFQKSVDVTPEMAKCWIDYLQTHRLPYVVAPFEADAQMVYLEQQGFVDGIISEDSDLLIFGCTRLITKLKDNGECIEICRNDFEKLPRKFPLYELNMEQLRILVCLSGCDYTDGINRVGLNKAMDFVKKHNTMEKILLAIQRDGKYEVPKNFMDEYKRATICFQYQRVFCPKLQKLVTLNELPENPLDVNSKFFEYIGYGISVETGLKEYLCDLEMIDHNAHYLISIGEKTPGNHKRDLINREINVTQSFSNAISMSTSTSTTTIGAGPVGGNGDTFSQPTSNPAKRSITIDSFFLRSRDSLTCNASFESKQVANAAEVEREMDKNKPVASARKIGHKNLQHMLEKRNSLLETRSKLGISSLSIKQQLNNVIIDKRKLVPKTGISSSSKFFCTKAEKCKSTELKSEXIAMDNEEQKKTESLDIAGDQNSEAPKTASGLQIEEVMCPEPLAFEEKTDKKFDQHCQEEDFLDGSEDISFDVSRDLDEIEAENSNHLLSGMSSSKNASTDTVXDLKQNVEKTFVFNDDXEKDFVNKTGSQQLLNSRTSKRSANVMGFPVFGGNDNINEEYCEENAVNHENNSISIKNKPVKRSLVFSKFAYTNSLRNTKGDDNKGISDIDHDRIGDIKRVLRPKNPNVNISNNLVRKDVNFATIHVDTEEYNDTDQSKKLKVDKISIHSLQKRLTSSQTGLSSNATTSHHNALHCNNGHGLNKRQLFRSNTTSPIKSTVNKRIPSLSRFIYRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4L4</accession>
    <name>A0A1E5R4L4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Mitochondrial import protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3664</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4L4"/>
    <dbReference type="InParanoid" id="A0A1E5R4L4"/>
    <dbReference type="OrthoDB" id="5529571at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070096">
        <property type="term" value="P:mitochondrial outer membrane translocase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013262">
        <property type="entry name" value="OMP_MIM1/TOM13_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28241">
        <property type="entry name" value="MITOCHONDRIAL IMPORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28241:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08219">
        <property type="entry name" value="TOM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ81857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="102" mass="11560" checksum="9511E25FC2E835D8" modified="2017-01-18" version="1">MSILEEVTIENTTTTTTNNXNNNNNNNISSSSLVSNTTWEKFMSIISSCSINLFLPFLNGLMLGFGELVAHEYSWKVGIFKRTRENSPNTFKIYKKPVSETF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4P1</accession>
    <name>A0A1E5R4P1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Dolichol-phosphate mannosyltransferase subunit 1</fullName>
            <ecNumber evidence="16">2.4.1.83</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AWRI3579_g3505</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15 16">Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O-mannosylation of proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a di-trans,poly-cis-dolichyl phosphate + GDP-alpha-D-mannose = a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + GDP</text>
            <dbReference type="Rhea" id="RHEA:21184"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 16">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of the dolichol-phosphate mannose (DPM) synthase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 16">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.83" evidence="16"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4P1">
        <property type="interactions" value="852"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4P1"/>
    <dbReference type="InParanoid" id="A0A1E5R4P1"/>
    <dbReference type="OrthoDB" id="2603at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004582">
        <property type="term" value="F:dolichyl-phosphate beta-D-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06442">
        <property type="entry name" value="DPM1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000119">
        <property type="entry name" value="Dolichol-phosphate mannosyltransferase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039528">
        <property type="entry name" value="DPM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398:SF1">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 16 19" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="18">
        <location>
            <begin position="8"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU365083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OEJ81887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="266" mass="29650" checksum="40B1CB865102EA75" modified="2017-01-18" version="1">MSTXIENSVIVPAYHEKLNIKPLTTRLFAALGNEESKKTELIFVDDNSQDGSVEEVENLQKQGYNVQIIVRTTERGLSSAVLKGFYDSKGQYLVCMDADLQHPPESVPQLLKSLRTHPFVLGTRYAPGVGIDKDWPLYRRVISSGARSLAKPLTVASDPMSGFFGLQKKELLAAPQGSVNAKGFKIALDLLVKLPFDDKLIGEVPFSFGVRTEGESKLSGKVIIQYLVQLKELYVYKFGANNLMVFLFIWAILALYVLFKLCQLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4S9</accession>
    <name>A0A1E5R4S9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADPH-dependent methylglyoxal reductase GRE2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3681</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4S9">
        <property type="interactions" value="268"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4S9"/>
    <dbReference type="InParanoid" id="A0A1E5R4S9"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05227">
        <property type="entry name" value="AR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000191">
        <property type="entry name" value="Methylglyoxal reductase (NADPH-dependent)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="6"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ81902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="342" mass="37852" checksum="AF19AB4211F2FCAF" modified="2017-01-18" version="1">MSKQTVFISGATGFIAQHIIQQLLDTNSFKVVGSVRNLEKASSLKANFKNHPDLSFVTVGDISEPDAFDKIFEEHGSQFDYLLHTASPFQFNVSDYEKELVVPAVNGTRGVFKAAVKFAPNVKKFVVTSSFAAIGAPADYENSKIVFNEKSWNNQXLQEALNDPVSAYCYSKAAAEREVWTLQKELKPKFAITAVNPVYVFGPQCFDSSVTKTLNTSCELINSIVHSKPEMPVPEISGAYIDVRDVARAHVESITNEKTDGQRLFMSEDRFTMQNVADVINKDFPQLQGKISKGNPGSDKAVIERMAQCDSSATKTLLGFKFKDLEETVVDTVKQILKVEAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4X4</accession>
    <name>A0A1E5R4X4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">40S ribosomal protein S5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3532</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4X4"/>
    <dbReference type="FunCoup" id="A0A1E5R4X4">
        <property type="interactions" value="1055"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4X4"/>
    <dbReference type="InParanoid" id="A0A1E5R4X4"/>
    <dbReference type="OrthoDB" id="10264639at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14867">
        <property type="entry name" value="uS7_Eukaryote"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.455.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="entry name" value="Ribosomal protein S7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_uS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020606">
        <property type="entry name" value="Ribosomal_uS7_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_uS7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_uS7_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005716">
        <property type="entry name" value="Ribosomal_uS7_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003106">
        <property type="entry name" value="PRK04027.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01028">
        <property type="entry name" value="uS7_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="Ribosomal protein S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00052">
        <property type="entry name" value="RIBOSOMAL_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS7" evidence="5">
        <location>
            <begin position="70"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ81959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="229" mass="25070" checksum="7F5958C8FD0C2893" modified="2017-01-18" version="1">MSEEVPVQETPVEQVEQVEIVETVAPVVLATEIPADVAAAQNEVKLFNKWSFADVEVKDPSLVDYVQISQPIFTPHTAGRYANKRFRKAQCPIVERLTNSLMMNGRNNGKKLKAVRIIKHAMEIINVLTDQNPLQVVVDAIVNAGAKEDTTRVGGGGAARRQAVDVSPLRRVNTAIALLTIGSREAAFRNIKTIAETLAEELINAAKGSSTSYAIKKKDELERVAKSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R516</accession>
    <name>A0A1E5R516_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein STB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3753</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R516"/>
    <dbReference type="InParanoid" id="A0A1E5R516"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="473"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="599"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="284"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="351"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="475"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="506"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="602"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="628"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="662"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="695" mass="76566" checksum="156158105826DABB" modified="2017-01-18" version="1">MTSVNVKSSDATKAKPLTPEKEKEITEKIIHRAELAKMARQLKFELGKVQSPTKKLRGKPLLLPNEVXMTSYSSPDPQGLKKEHRSSSPMKRNKHDELQYGTSNHIAGNNDGRESSSPTKKYKHFVEPTTPDGSTAYFNQLRKGDGRRQQVALKPHSKVRQQNSNNHNKDHLSERYVHAGQTLTQGSYLPADNVNTDDISRNNDPEMSNDDSFQKELGADLLMYLASSPYNSMNAAPSSVLKSIDGNQHLQHSSASINNYNILQTPSRNFGLEGLMASPGGGNTNTRLNGNNDGLNLLHHSSSDTNSNSTIDDHTDNSTKEYKQTHKNLKQQLQKEVIPMTPLNRVRGKSSRISQKSDTRGISQKKNQRNKVFDDDDDDDDEDEDNGDVIEENENDVTXDETDVEEKIRKSTTPTDLFNGDHEAVIGQENNYLNSHQFINKQAGQMMISSSPPREFDSPSVYTQLMLFSPAKANKGSQNAADRFEQTQGATIRSDRTEAGHSLGYMRRESGSRNGDEHKFAANSNLLKTPSRPINGVSGNTSGFFLPPNSATANFMMMKTPNTQQLASISRLLLDSGGGSATAQSNGIESHLEKLSAATRAVEARDDAGEQGIESRNKGAPHLESARDNAAANSDASKTLNSKTSSDGDSKDNLQNGHELNKNGQQNQNNGNFSMGEYMNNLFSPSPRVSSHRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5C9</accession>
    <name>A0A1E5R5C9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription activator GCR1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3650</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R5C9"/>
    <dbReference type="OrthoDB" id="3973223at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022210">
        <property type="entry name" value="TF_GCR1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12550">
        <property type="entry name" value="GCR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription activator GCR1-like" evidence="2">
        <location>
            <begin position="1107"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="385"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="746"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="883"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1044"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="304"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="315"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="400"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="440"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="469"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="498"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="537"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="555"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="572"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="754"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="883"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="913"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1044"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1064"/>
            <end position="1077"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1211" mass="134090" checksum="24F46A85E4CE97B8" modified="2017-01-18" version="1">MRLVGNDVDNVVNTKDIIDIKKTQGPFVSSKESSECERSSQRGSEDDEKYTNEGNHKQRKHGKQRKRSREYEDDQDATNRKLKQKLSKTSMNIDKSHDPISNLNKXLGFLSESDDLDTKMRHNNKNDKIEDDIGNDGEPAIATVRNLEEQKAYEFSLQRQIQLLEKKLESLKRKVTYKKTTYDRICKNLHVIRELLWNSYNKYSESSSDKTKNRNKEKIRKRNSLVESLIKANQNLNTSPNNPGVNRSTDSQGSLEHQNVTDYINSLHIKDLFSTEENSDNNGSDNSSSDNSGSDNSSSESDNPHVSQSKDVAQSLSSSKSKTTSSTLKKRINAVGSDSDEHDGRGTRNSLSHEFSYYKNPGSSLSMPNLSSSSKVENLLAQNAHITLRRSNSRPPTFLSKGTXTTANNTASKTRGKVQTDNSNKNSSDDQDNDNSTGIKKNERGVHNNXKSREYDADDDNDDFHRENDDYDDDDDDDDDDVDDDYDAGNDFSHDSGHQNVSPRSQVYSSDKNPALSKNAKHNKKYTAERPLISATNEKDSALSKDVIKNSTGVENNSXTNDSTETNSTSNLGTNSHGVTSSNDENTISANSLKSTDNSKEVIDNSSLDEDSAKTSESPPIKPKHIVNPIKDGYIKAEANTNPPSYNIRNSAQTSSPQNMVVYLNSPHETKNAGHSNMHLKRNSTNMSMTNNSCSENNEDLGYTENSVDQNLHKQGQNAIRVTLDGSNSLSMINIKNRHGLNDVNVDDKHDTVNNDQIGSKSNRTTARNEELIYENFVNDINSSNSTEGNPVKNNNNNITTTYHHHHHHHHLLHQSPWNLGPNQQLLSQNYYSPSDNSNGNDLMIPKATNIQSFPYTEYNAPNSENQLGTNAVTTITTTTTSKVNNVSDSKRNSSSASSNGNNYXTVERPHFASSSGAPPMXSTTSNVANPTILPAPPSTFGXQHHMGVSSALNTVGNVPGQDAAFSNASLAANNAVVSEQPRQHAFQVHNQNSASLWNPNTGNNMDSIALSNAGAMNDENLMRSMNTPVINAEINANANTLRGSVSQRGSSGQGTNEHEDRSTSATSNNSGKSIAGSNAGIGTRGASTQVGTLTESDVFERWGVFFNERIKKVEDVWAEYHRVSEKGYSFEKLDLLWSNKWKLHCEPKFVKKFNRRLVMIKAIQNAVNETGDSASRFVNFMDQVLIDRKKPISHFYKKTNIPNLLKSSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5E6</accession>
    <name>A0A1E5R5E6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Golgi apparatus membrane protein TVP38</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Golgi apparatus membrane protein tvp38</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3586</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Golgi membrane protein involved in vesicular trafficking and spindle migration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TVP38/TMEM64 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R5E6"/>
    <dbReference type="FunCoup" id="A0A1E5R5E6">
        <property type="interactions" value="110"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5E6"/>
    <dbReference type="InParanoid" id="A0A1E5R5E6"/>
    <dbReference type="OrthoDB" id="166803at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000022">
        <property type="term" value="P:mitotic spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051076">
        <property type="entry name" value="Golgi_membrane_TVP38/TMEM64"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="VTT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47549:SF1">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47549">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP38-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="VTT_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="231"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="262"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="295"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="VTT" evidence="11">
        <location>
            <begin position="164"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF09335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ82124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="331" mass="38066" checksum="6A9E38D306C19E48" modified="2017-01-18" version="1">MADQYEARVSNNMGQNLFEDDVDDADFFFSNETSVSPNRSTNFNSIFNNDFLVNDDDDDDDDDDDDDMLELYNLTPRQRLYYKLKHTLWTKNVKKFKKLAQWQKIILIVLMFLFVLFVLTFAVMHKKILEYLVETSNELYTKWYTSWVLFALIYVVAFPPMIGYSLLSTSVGMIYGVSFKGWFLLASGTILGSVSSFYVFQKFLNNKAEKLVHSNRKFEAIASVLQENNSYILLALVRLCPFPYSLTNGALAGIYGIKLKNFIIANCIVSPKLILYLFIGSRFKNLGEENNPNKVFDLLSIVFALAVLSVTAGVLYSKTKKRYRELQSDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5U3</accession>
    <name>A0A1E5R5U3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Increased recombination centers protein 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3680</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Is probably involved in a pathway contributing to genomic integrity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the IRC22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5U3">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R5U3"/>
    <dbReference type="OrthoDB" id="1926781at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005595">
        <property type="entry name" value="TRAP_alpha"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03896">
        <property type="entry name" value="TRAP_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184636" description="Increased recombination centers protein 22" evidence="11">
        <location>
            <begin position="20"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="79"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ81903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="343" mass="38162" checksum="E2B350113E5F154D" modified="2017-01-18" version="1">MKISIAALAFISLSAAVSAQESYSSEPTIESQTVESLSLDDSTTLAEAETKTAMNLQEQTAEEPVMQQPIVEEPIAEVSAVEETAVEDNDADDADDDEEEEEEDIVVVDQEEQTPVDIVTEAEAEETPIVDEAFLQNKVLNEEAIPENIIKVEQEAQQRVNFTIDWVFVDTPGNGLDNYYEFSASETGLLNYTFTNYENEDTLIYAVSGQIIEPEKYEIVANITETEIGPIYVGVNETVSFLQKLELVLPEGVFFVTPMIHFGEAEDPKRVGAAPKAIRILPAPLSFFNPEFLTILLTLGVISYYSYKSVYSYDKKPNKKALKAQARDAKIDAXXWVPDNHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7E4</accession>
    <name>A0A1E5R7E4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flo11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3308</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R7E4"/>
    <dbReference type="OrthoDB" id="4070545at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018789">
        <property type="entry name" value="Flo11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10182">
        <property type="entry name" value="Flo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01213">
        <property type="entry name" value="Flo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51824">
        <property type="entry name" value="FLO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184631" description="Flo11 domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Flo11" evidence="3">
        <location>
            <begin position="20"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ82814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="313" mass="34378" checksum="B7E0C00A6E10234D" modified="2017-01-18" version="1">MLFHNTPLAFVIFFTVILRLVAAGPTCTPFKQCQQNNLWYFQNVGSVPYYFELTNVEYVSGTSYSITFHVWSDANIPLTSLNELKFLGSGIQSSDVMIYSLNTGAGLNDPNFNPTDFTKTITVDTSSANYGSSLTGLPAGFGFQFDYCKGMNTYSTPKNSAFYSGYDYTCQCXWTYGATSFDYYTSCNGDNNCNSQGAFSNWLWPQQCGSLSDQTKKSQISSLSAANPYSYCTYXGTANADFTTNLKVWWTPDVCNSYTPTSTSTKPTTTSTSTTPTTTSTSTTPTTTSTSTTPTTTTCTENYFCNNLFSGYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7P5</accession>
    <name>A0A1E5R7P5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sporulation-regulated protein 28</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3186</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7P5">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7P5"/>
    <dbReference type="InParanoid" id="A0A1E5R7P5"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031105">
        <property type="term" value="C:septin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="6">
        <location>
            <begin position="11"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="332"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ82932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="384" mass="43168" checksum="4887A213300C3604" modified="2017-01-18" version="1">MSFVEAMSXKKTKQLGVLIAGESGLGKSTFVNNLCNRDVFQETNIIDFENAHLDPGLEIFSRQVQINDKNRHPVDLQLVLVNGLGDNIDNSELPGTVASYLDKQFDVVFSEENXTSKLSRSIDTRPHVCLYFIRPTSRGLRELDIISMKALCEKVNLIPIIAKSDLLTQEELYLNKKLIMQCLEDNKVQIYDFAEDTLKSIEKNDNLQVILEASHAASTKKISKALPFAVIASNDLQVDETGNTYHVRTYPWGTVKVEDPNVSDFTLLREVLLSSHLEKFKTRTHEVLYKNYKIGKLLKCKXPKGSSFSIASTXSFQRDSLLCESTTPLYTSGSHSLSVLDNPRNSKXDDKSLSDKDDLIFAYEQKILRLQKLLKSSSATLPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R843</accession>
    <name>A0A1E5R843_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Iron transporter FTH1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3281</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxidase-dependent Fe transporter (OFeT) (TC 9.A.10.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R843"/>
    <dbReference type="FunCoup" id="A0A1E5R843">
        <property type="interactions" value="81"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R843"/>
    <dbReference type="InParanoid" id="A0A1E5R843"/>
    <dbReference type="OrthoDB" id="4364at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033573">
        <property type="term" value="C:high-affinity iron permease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004923">
        <property type="entry name" value="FTR1/Fip1/EfeU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31632">
        <property type="entry name" value="IRON TRANSPORTER FTH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31632:SF7">
        <property type="entry name" value="IRON TRANSPORTER FTH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03239">
        <property type="entry name" value="FTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0410">Iron transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="203"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="349"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="384"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ83076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="426" mass="48150" checksum="9612CB9B21E294BD" modified="2017-01-18" version="1">MELTNYFSFQIFFIFLRECLEIAIIISILLTIVKQSIFPPESNDHGVTVKSAVPTTLEEEELEYSLQGNALSSSATPGTYSDQESTEQTIDREHLYKKLKVQIFAGGALGLLCCMIIGTIFITIFYMVGTDLWSKSEHYYEAFMSILASLVISIMGIFFLQIGKLKEKFRLKLARAIYKTEQNKKQSLFDSANSLKYTEKYSLFLLPFITALREGLEAVVFLGGVGIDSPLSSIPLSLLAAATISYFFGRVFYTSSTSFSLKICLIVATCLLYIISSGLFSKGFWQFDVQKFVDRCDGQDMTEMGNGPGSYDIMDSVWHVNCCNGERDGFWMVASAILGWTNSATYKSVISYNVYWLGIIAVFNAMKIIDKYGYIPGVPIFLQRKAINKKIAFLKQRLEDHQQQQQQQQQQILGPVQEHLSSFENR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R881</accession>
    <name>A0A1E5R881_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Translocation protein SEC66</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3336</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R881"/>
    <dbReference type="FunCoup" id="A0A1E5R881">
        <property type="interactions" value="57"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R881"/>
    <dbReference type="InParanoid" id="A0A1E5R881"/>
    <dbReference type="OrthoDB" id="73168at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031207">
        <property type="term" value="C:Sec62/Sec63 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031204">
        <property type="term" value="P:post-translational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018624">
        <property type="entry name" value="Sec66"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28229">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28229:SF1">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09802">
        <property type="entry name" value="Sec66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="196" mass="22943" checksum="0A578B3E214A5C6F" modified="2017-01-18" version="1">MSTNSTFEETFEETAKASAASLKSKISVYTPFIYLAIVIISLYVFSSRYRKRELVKKATLPSIFEDDEPRQLYYEIKALKQDPEHKVHDKVLQAALLNRGAEAIRRTFKLKESQPQLEILYKMGVMGDEFHERCEREMKLTDLEFKECVQESETLQPGFAQTFVNLSREICFNEALRRRYNSIEIRKKVLNAQLEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R8R5</accession>
    <name>A0A1E5R8R5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein in HIS3 3'region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3359</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R8R5"/>
    <dbReference type="FunCoup" id="A0A1E5R8R5">
        <property type="interactions" value="145"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R8R5"/>
    <dbReference type="InParanoid" id="A0A1E5R8R5"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070124">
        <property type="term" value="P:mitochondrial translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016712">
        <property type="entry name" value="Rbsml_bS1m-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28058">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN MRP51, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28058:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS1M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11709">
        <property type="entry name" value="Mit_ribos_Mrp51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ82923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="342" mass="38867" checksum="1B6FEB862B492485" modified="2017-01-18" version="1">MSSLSELLRGSRLSQVPLRKKVPLVNPPKKFYPQHQIIATKPNTMKEFKEWGLKAAIPSVKSKTKYIVVNELDNKYRMTEFEPISGGFNWNRLRLQELNIAPQNIHKKNPLFFENEFKAPSLKALLDSKAKREYLKSDKFRSEFKTWLLEKYPNSLYQRGFHFKNLEKETLEFLQDRFGSHKHSMSNNIVGCGGLSYNLKGRVANTPNGFVLNPIVAGRILNGPSSSERIVSVAGFAGVGRNNEKLDKKDKDFGRAAFRQNKVPIQIKDYKVINDGSRLNSELRVNILDDVNLHAGSAGVNNRKKFFNLGAQRSKPSSTSVNDSAQSKLQIMEFLSGMMKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R960</accession>
    <name>A0A1E5R960_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3285</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PGA52 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R960"/>
    <dbReference type="FunCoup" id="A0A1E5R960">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R960"/>
    <dbReference type="InParanoid" id="A0A1E5R960"/>
    <dbReference type="OrthoDB" id="118256at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018805">
        <property type="entry name" value="YJL171C/Tos1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018807">
        <property type="entry name" value="YJL171C/Tos1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31737">
        <property type="entry name" value="PROTEIN TOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31737:SF2">
        <property type="entry name" value="PROTEIN TOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10287">
        <property type="entry name" value="YJL171C_Tos1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10290">
        <property type="entry name" value="YJL171C_Tos1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184647" description="glucan endo-1,3-beta-D-glucosidase" evidence="9">
        <location>
            <begin position="22"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="Cell wall protein YJL171C/Tos1 N-terminal" evidence="11">
        <location>
            <begin position="38"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Cell wall protein YJL171C/Tos1 C-terminal" evidence="10">
        <location>
            <begin position="303"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="230"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF10290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ83073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="545" mass="56666" checksum="653126235E63D944" modified="2017-01-18" version="1">MKFSNVAKLSAVMLVSQKVVADDCQYVSGNYYCSEVEAVVYNNVGYSGSYSDVTSMDESSCVCSQSSTSFSGTNAPLDEELSVHFRGPINLKQFGVYSLSSSSSSSKKKRDSNDANHKHGNLEERAVVVDVVEVYSTVFVDSDGNTVTPTETSTVASTEDATVAAATTASTASLLDENEVQGSSQLTTTIVQVDTSSVSDTPATETEASSTSSISVELTNYAANVVVSTTTTSSSSENYTPTTTVITPSSTSSTEESSTSSTEESSTTSTEESSTSSTEKSSTSSSTSTSSSAAATTSSSSSGSWSRSAYYEAGSSADNVVFMNYYGGSGSGVWSSCFGNSISYCNSDASAGSSSAVALSDVTVESDTEFMIFSGESCGSSSDCGYYRSGIPAYHGFGGASKIFVFEFGMPTSTKSSSSNNDMPAIWMLNAKIPRTLQYGNADCSCWSTGCGELDLFEILSSGSDKLISHIHDGQGKDGSSYGGGGSQDYFTRPTSGTMKAAVIFDSDSSIHIVQLDDSVTFGSSLDDSTVEEWLNKSGASATLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA98</accession>
    <name>A0A1E5RA98_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">SNF1-activating kinase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2835</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA98">
        <property type="interactions" value="200"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA98"/>
    <dbReference type="InParanoid" id="A0A1E5RA98"/>
    <dbReference type="OrthoDB" id="68483at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14008">
        <property type="entry name" value="STKc_LKB1_CaMKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF152">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE TOS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="208"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="30"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="106"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="567"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="642"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="872"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1049"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="595"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="656"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="709"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="872"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="892"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1083"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="9">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ83801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1146" mass="129643" checksum="86825995B630ECB4" modified="2017-01-18" version="1">MNETTGNDQPEMVDLPAHLQLSLKQMSISDLKSATPTDTQEYAPFKQKLLPEQQEDTSSLKNSTHAKKAEQDTHSKSQTIGIPQGVRRGNNLLVLDNHFAKREARDTKNMKNDITSSTYKDYDEADDDDGDDNDDDDDDDGNYNNIYFGSSLDSQSSLESLNLILEKQRKQQLNNPFHQTHIFYPVKETNKISLEYDPVLKKTVLNHYEIIKELGHGQHGKVKLAKDYYTSKLVAIKILDRVEPMRNKFKLSNKNAXANGLIENISVKREINIMKKLHHEHIVKLIEVLDDIESRKIYLILEYCSKGEIRWCLGDQLEIQARGPPLLDFDQARKIFRDVVLGVEYLHSRGVIHRDIKPANLLLSDDNVVKISDFGVSLAVQNCNEEDMCKTVGTPAFYSPEICLGMDSALEHFGGTPKTIISYPLDIWALGITLYCMLFGRLPFICSHEMELYDKIINDKLDFPKNMAAFNVDSIEYHQAQNLLLGLLEKNPYKRLTVRDIKMHPFTLLKLSPTEQEFFLKSQYQLSDDMLFMDSEQFDSLNENSKXGYNDLRSIDKSITRDIVDQEVKRNQFQNDQKSHHRRSKSNTNTSDKHHNSHHHSQNRKGHQSNFVSLPVNSSFASLDSFYIDSYAQAREHPFNFMNSGIDQRRSHRKNSYSSETPSSTILRRQRKNSNDFIDDKSSKVPNNLKLNFNPIDIPQNRLLQLQNPKHREKSKKPGKSKLKPFGDSNEDXHXISNYNAEPFKKLGQPHKSLRRTSSVSYADISNSIPSSHAHYLQGGSSYSSSTNDLTKAASVSSDSSSEYAYGYYASAGHRYPEPTSETESLPFEFRVDSAHNSIISLNQMVVPIAQKLPENDSNLPAGAVSVADFHELDTNNPKPENKTKHATYRFSSSENESTSSYTETASSTSFTDDEDKDCFANLEPKELPNADQIFKSAECGEEEYFADNAESHPAYDDSRDEFVMSSVRTLKNPPNKTPLLSYLQSNHXHXPLEHPDLEKSAATISMAELSERPSMDAALMHKDNSVSTLQDVEQLDIPEDILMKLNPSVSARPNEPAKLNIPEPRVLPGGPSTAPSSSSSSLMKQTLLNSRTTLTSGSSSARVQDNRNTLPTTLTNDVGVLSERTRNDFKSVDLLHSVLKRKDQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAB1</accession>
    <name>A0A1E5RAB1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">40S ribosomal protein S26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2822</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes). eS26 interacts with eS1 forming part of the mRNA exit tunnel. eS26 interacts with TSR2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eS26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAB1"/>
    <dbReference type="FunCoup" id="A0A1E5RAB1">
        <property type="interactions" value="784"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAB1"/>
    <dbReference type="InParanoid" id="A0A1E5RAB1"/>
    <dbReference type="OrthoDB" id="10262653at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1740.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1740.20">
        <property type="entry name" value="Ribosomal protein S26e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000892">
        <property type="entry name" value="Ribosomal_eS26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047864">
        <property type="entry name" value="Ribosomal_eS26_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038551">
        <property type="entry name" value="Ribosomal_eS26_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12538">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12538:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01283">
        <property type="entry name" value="Ribosomal_S26e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00733">
        <property type="entry name" value="RIBOSOMAL_S26E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="91"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00065030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ83834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="120" mass="13393" checksum="6597B18EEB013B72" modified="2017-01-18" version="1">MPKKRASNGRNKKGRGHVKPVRCNNCSKSVPKDKAIKRVSIRNIVETAAIRDLSEASVYAEYALPKIYNKAHYCVSCAIHARIVRVRSRVDRRNRAPPQRTRFARAGAKVTPGDAAKKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAN3</accession>
    <name>A0A1E5RAN3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">40S ribosomal protein S24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2867</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAN3"/>
    <dbReference type="FunCoup" id="A0A1E5RAN3">
        <property type="interactions" value="1179"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAN3"/>
    <dbReference type="InParanoid" id="A0A1E5RAN3"/>
    <dbReference type="OrthoDB" id="5571754at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3370:FF:000001">
        <property type="entry name" value="40S ribosomal protein S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00545">
        <property type="entry name" value="Ribosomal_eS24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053709">
        <property type="entry name" value="eRP_eS24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001976">
        <property type="entry name" value="Ribosomal_eS24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018098">
        <property type="entry name" value="Ribosomal_eS24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10496">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01282">
        <property type="entry name" value="Ribosomal_S24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00529">
        <property type="entry name" value="RIBOSOMAL_S24E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="97"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="99"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="118"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ83969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="129" mass="14767" checksum="014BCACD29BC9BD7" modified="2017-01-18" version="1">MVCTTSHLLEYTFRKQFVVDVLHPNRANVSKDEMRERLAEIYKAEKDAVSVFGFRTQFGGGKSTGFGLIYNSVADAKKFEPTYRLVRYGMAEAVEKASRQQRKQRKNRGKKVFGTGKRLAKKVARRNAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAP8</accession>
    <name>A0A1E5RAP8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">R3H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2900</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAP8"/>
    <dbReference type="InParanoid" id="A0A1E5RAP8"/>
    <dbReference type="OrthoDB" id="278430at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02642">
        <property type="entry name" value="R3H_encore_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="R3H" evidence="2">
        <location>
            <begin position="22"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="536"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="133"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="252"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="282"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="404"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="453"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="536"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="558"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="597"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="607"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ83979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="650" mass="72509" checksum="1A3AF14C0F86310E" modified="2017-01-18" version="1">MTLILGNLSQALRIALIKPEDREYITQLEENILNFLNHKELQRLYLEQDLNSYYRLLSHQVAEIYELDHVLIKQENRILLYKQPKEYAENHIISLKDIPIKELKESLPGSIMESIATTITNDGSDEMELIDGSEQSETSETSKTNKPNTEKNGQAKPRSGKFTRTRSISPNPRDFESSSYRIERAERANHRYTKPQNPTYQYFGNNNSYDNFGEQSQNFGDQSHNYNGYSHNNQRRQYVPYATSSGANSPRTFSSTNNGNNSFNNYKKQYNWNKNGSANMANNNNDNNNNNNNNNNNNNNNNNNNNDNNRNWKQWNRKNSKTLTNFQIQNYPAQYWDPSGAPNGAPNGAMMGGLPPTHPMYIYQPQLMPVGAGNDGPMYQDMGMIMANPLQNAVPGGMADFTHPXPPPPPPFVNEPYYSGNKTPPRTSRVSSSGMPSSTGTNNSTGQDPLAVSSSETSSPQPMSRPFFSGGSPNGLPQHHQHPYGVPVQIQFHPSGMQYPVGFPPGSSGFQGQAPSIGMYVHAGYMPGAPMSNGNMGANNGVNNRNNSYNRHSRGSRQGSYQGRTSRKQSFQNSKGLGIKMGQRDEGAVSPQTQENPTKDSVKNKEEEEAEEGAEEGAEEXEAEEEEAEEGAEEEAEEEAEEEAEEEADN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAV1</accession>
    <name>A0A1E5RAV1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA endonuclease SAE2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2844</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAV1"/>
    <dbReference type="InParanoid" id="A0A1E5RAV1"/>
    <dbReference type="OrthoDB" id="5801062at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="246"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="434" mass="50671" checksum="C77DB81CA9725D55" modified="2017-01-18" version="1">MEFKSGCAFMQTLSLEELLDVQEDVVNLIRKRVLEQKXNLRQKIXSPLQQPDLPVQXFCELPGEPLLKEEVIESSQNFDPTSFPIGTLXSASGSSTQECVRTHLYQDSSQPEKNFSRIEETLGVRKIKSAHLDSSPYKHDTTFGDGVSELRLKEPELIKHDLKFQHFKSPNKKMKKETSPAKKSLSDGLIHSPYNLQSEPLLKQSXVNATPASLXFPDDKDSQEYQDELLPSASQLPPVIYKLTDLRKFQLRRDELRKLLDNAQAKENNNNNNNNNNKSFPQFVNLNRNPITLGEWRIQDFVANEKSLAHHISTKKRSRKYHEEKAWEQFQKGEDVDKRYLYMFDNMKDREPEPPGYWRIMWPSTQENEQDKREMALVESQNIEDRFHKAVNYKIPEEEREFVFKEELLNLVVNNGRFHFQKHNLGIKSKKFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB29</accession>
    <name>A0A1E5RB29_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Casein kinase II subunit beta</fullName>
            <shortName evidence="3">CK II beta</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2524</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 3">Regulatory subunit of casein kinase II/CK2. As part of the kinase complex regulates the basal catalytic activity of the alpha subunit a constitutively active serine/threonine-protein kinase that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the casein kinase 2 subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB29">
        <property type="interactions" value="1057"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB29"/>
    <dbReference type="InParanoid" id="A0A1E5RB29"/>
    <dbReference type="OrthoDB" id="3971593at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032545">
        <property type="term" value="C:CURI complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030291">
        <property type="term" value="F:protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060962">
        <property type="term" value="P:regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006356">
        <property type="term" value="P:regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006359">
        <property type="term" value="P:regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1820.10:FF:000005">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.20:FF:000001">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1820.10">
        <property type="entry name" value="protein kinase ck2 holoenzyme, chain C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016149">
        <property type="entry name" value="Casein_kin_II_reg-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035991">
        <property type="entry name" value="Casein_kinase_II_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000704">
        <property type="entry name" value="Casein_kinase_II_reg-sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740:SF39">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01214">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00472">
        <property type="entry name" value="CASNKINASEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM01085">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57798">
        <property type="entry name" value="Casein kinase II beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01101">
        <property type="entry name" value="CK2_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="61"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="88"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00045899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ84105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="356" mass="40675" checksum="2B547AD7A7CF3FDE" modified="2017-01-18" version="1">MATTQQSXTTSDKKTFNGAGEADFEITHKENAVPESSARSHSNLDNHKEQSSSINTEANPQQQQQQQQQQHSQDQVMQEEVDHDSLGSDESMTSTSSSSGYLEPWIDGFLGKKGNEYFCEIDVDYITDRFNLMGLNRHISTRFSTVINFIVDELDDEQLSKLRGSDYEQLCKDAAKLYGLIHARYIITLKGLDKMMHKYKEGDFGKCPRVFCEGQHLLPVGLHDSYSKEFVKLYCPCCEDVYVPKSTRHSNLDGSFFGTTFPGIFLQQYPKLVPVKPIDNYVPKINGFELHRHAQWARWQELQRLKHLRLLKETYGVKNVEGGYDVPGSYHLQPLVNSETIYSSEKPRVVDLSDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBF5</accession>
    <name>A0A1E5RBF5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inner membrane assembly complex subunit 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2478</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBF5">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBF5"/>
    <dbReference type="InParanoid" id="A0A1E5RBF5"/>
    <dbReference type="OrthoDB" id="2253354at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="34470" checksum="E77C69AC01EB4F9F" modified="2017-01-18" version="1">MRNVGHFVSAIRYKLIFPGPSTVXXVSATIPISKYPSNHKEEPVRIQLNMLIFSKLNKAQVFPKVPRGPFINSKFSGLNKNIWGSVQNVRSLSKAFPKQAQEGTGKESKKKSRFDKSLVKPLLLILVFSSMLNNITEQRKKNAALEKRYNLKMEKYDELMKRAQTTGQTVFDLEKEFKLIDKMFERSDKFSFSPNIPNAKQASPLVEETEEDLSKLFSEIMKDADKTXEELQNEHLANSIALKGEDINADEIVTNKDYLHQENQLEQKIMKSYKADTSQHILVENSGDYANAPTTSKVKKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBI2</accession>
    <name>A0A1E5RBI2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein MGA2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2468</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBI2">
        <property type="interactions" value="1483"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBI2"/>
    <dbReference type="InParanoid" id="A0A1E5RBI2"/>
    <dbReference type="OrthoDB" id="3972546at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00102">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057962">
        <property type="entry name" value="SPT23_MGA2_DBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23335">
        <property type="entry name" value="CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR CAMTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23335:SF1">
        <property type="entry name" value="CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25603">
        <property type="entry name" value="SPT23_MGA2_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1382"/>
            <end position="1401"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="5">
        <location>
            <begin position="869"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="1044"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="1077"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="101"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="167"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="538"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="621"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="756"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="820"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1180"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1230"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="185"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="200"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="543"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="625"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="650"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="664"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="820"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="845"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1184"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1234"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="6">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="6">
        <location>
            <position position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ84258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1471" mass="163918" checksum="C45ACB94BE3392B6" modified="2017-01-18" version="1">MTGSSDNNHTDKNGLDKIFQEMDNDIGDGFMQNDLPPNDINNGVMQNDVNSGIMQNDINTDLPPNDNEGDMLFNQFIKVENGEESYHTPMQDTNEVIESNQNVSSTNNGNGSNENGVSSSQNNNTQGNISEQLSYENYEQKIFEDFLKQNSDDLSPLNMNSFFEKETKPSLQSVGNGNGNGNGHLSEHSMKQSVEHSRQHSNGFHQNSNININYNDQSKLSPTAQLNNNTTPSAQFKNMNIDLNMLLNSPNLAHLKAEFQDYLMNLSNGNSPLMMNNSPQIINNSPQIINNSPQIMANNSPQMMMANNSPQIMMANRTNNGFGQPPLLQDQQHGTTNQPSNVTQNTYHNNNLNNNLDSNTVKIYKEQCAEMIKSLEFGRTTEIHPIDPYQQKQEQVDFSNTGNSNFAENYPFEFSVSPLPEKSRVETQVKISFKVTPSPPLNILHISNDCITKQKQLLKQDISTYPEQLRKQILYLDAQVVCATKNKPVGVCARCLKREQRRSSRRKSGTSDINLWCNNENRKIIVFNNKQLTLLHSPKDXASTNNTSTTSVNSIHDNSNNGNTDYKSFDLSARLVCYCRHHQEPVGFKVVFVLKNYTGQIVAKTVSTVIKLIDKEPTKRKANGSISDASFSTTSDDTSSVATGDRRSSYESVGSSVSESESGFNETNTNFNGMNPIKLDTTTADLLQQHSPSLNNSNLNLVGMNLQNSSTNMQFNNIAATLXRKNNNQLGHDHLSNGSSSIASKFSNNMKFETTIPSPSDYSSDANASGNYAQGQHQGLFLPSSVGKSARNNIYQANGFQPQQTGYQYQQQQQQHFQQQQXXXQQQQQQQQQNXHQHXQQQQQPFSLPNASPNGQVFDMDMLNAVNVLPTIQKIIPAEGPITGGVEVTILGANFKSGMCVKFGNNRALSASCWNGNTLTAFSPPASTPGPVMVSVFNEEDEMLSDQMLETNANPNAVFTYTDETDKKLIELALQIVGLNMNGKVENASNIAKRIVGDSETPNQSQPNNSQYSQYQHNSNQNLVAKVIKLVNNTTTNVSLCNKQGQTLLHLACLKSYIELILVLIKRGCRVEITDKYGYTPLHFACLTGDIRVLKILLTCKADPCSPISTRELLTPIELYTANHNASTTPNYQAILALLTPAANRKGSLSSLDSDILDSDSIYSNGSGFQVHISKMISDHLSEDEGDGEDDEDEGXYSLDDSECEEDLHITQNNPSLSQSIATEGEDNAVVKAQEQQQQQESSSTEDQHSTQSTESLPPQYEDLYPHKDKSFFDQEPHDFSLLNSKKLHALITLPVTPSSLPATPLTPVASDDEEQTDMENIRNGSYTLNIAGDETEASIEIPGNVAKDVNAEDDDEDDEDYENMISIKFLKQKIDFSNDKMLLFFWIPFMLVMLSSFFLFKFGSTDNRVHYLSERSSERIRIWLTKIVLGNKRMQTIFKDAFKEAFQNKGELFKDVFQQTVRHSVAVSPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBJ3</accession>
    <name>A0A1E5RBJ3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Flocculation suppression protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2660</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBJ3"/>
    <dbReference type="InParanoid" id="A0A1E5RBJ3"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="6">
        <location>
            <begin position="111"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="139"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="219"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="304"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="432"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="588"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="637"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="790"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="908"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="968"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1029"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="48"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="139"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="152"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="219"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
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            <begin position="304"/>
            <end position="318"/>
        </location>
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        </location>
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            <begin position="432"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="641"/>
            <end position="677"/>
        </location>
    </feature>
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        <location>
            <begin position="705"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="791"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="821"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="833"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="922"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="989"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1029"/>
            <end position="1041"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ84268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1048" mass="115077" checksum="F9ACD2F02E540F29" modified="2017-01-18" version="1">MSTSSSLNTNTPNNIYTNTKSSQVINTNEPERYNPPADGQPTAPIKLTNANTSTSTSVNNTQKGMQSQTNQSFTQKLYTMLSDESLKDLIWWSQSMDSFMIKPNERFSKALSIYFKHTNVASFVRQLNMYGFHKINTSESTTNETNASGTNQSRNISNPTSNTTSGKKKSNVSEDGINLWEFKHSKSYFRKGDLENLXKIKRRASKNTVTHVATSTTIRNGTAAGPSTTRPIINNHTTESSGAPTTRLHPLGYNPPLGNNXSLNNYTINHRNSYPGEATTNGSGNTVGLAPQTLTIHANESQTGINTKPFYSNTQDARSSSLNNLPAALPSANNSSTNIKNTSNNNNNNMSTYNKNNLHNSTLINNQFAHSLHRSSVTSVSSMPTVCNPPGVPANMDSHLMDPSKPRNNSVPANAYKDINNQLPXPMENYTTQSAHLQQSPTSFQRKSLSNSASESMSNMENSKMFQLVIDELQNISTDNIQILELFQYYLSEKRNDGNSNNTNYMNRSDALLNDLQSFKTNIANRSNKIIDLLKNKNMAYLQLNNNNLINNNNTVSNTASTTMGGNNEMVKSEQIGSAVHMYPNVPATVKTPPPPSLTSSSFTGMPGQVQGNSSLRNMSVIDPLQPLPSXQVQSYQPQLNAQHVQHAQHAQHVQHAQHAQHAQQANQTQQATTGQHEFTRATPQPQHYVPSNAPMSPFSVRTYEMSSSNPQVPPKNTENNLTSSNPTPQQQQHQQQVKNEPMAYKPYYPFGIMPLGAGYPKNDVQQKQQPHVQNYQPQLQHQXYVTPHQSQPVVNYPPSNNSNQSVVASQSQQQTPFFARTNSNPSNKLRISVSSSATTMSSSNDFHSHHNTNNNDNXAGNGNESRVSVSSLPLTMGTATSFLPNHQYIQMPSTQAIININDASSRKNSANNNAGAYSLPGLSQPNIPMQSVPQHNVAQPNFSLPGLPRLNSSVSASSSSTLLNASHLQHKPSVSGSNPHSKSDLYSLLNTRNDSSNTESAEQQHQLLQKLNNTSAQATIGMKRTYSDTYGSRAHSNNIDPKTPAPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBN9</accession>
    <name>A0A1E5RBN9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">T-complex protein 1 subunit delta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2887</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBN9"/>
    <dbReference type="FunCoup" id="A0A1E5RBN9">
        <property type="interactions" value="1454"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBN9"/>
    <dbReference type="InParanoid" id="A0A1E5RBN9"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03338">
        <property type="entry name" value="TCP1_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000010">
        <property type="entry name" value="T-complex protein 1 subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012717">
        <property type="entry name" value="Chap_CCT_delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ84301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="577" mass="62803" checksum="3ECD1D5684CB9B75" modified="2017-01-18" version="1">MATKAQTSTPSNSVFNNREKPQEVRKANIIAARAVADAIRTSLGPKGMDKMIKSSKGEVVISNDGHTILKEMSILHPVAKMLVEVSAAQDAEAGDGTTSVVILTGALLGACEKLLSKGIHPMIIAESFQNAAARCVEILHEMSHKIDLVKDRDALVRAASTSLSSKIVSQFSSKLAPLSVDCVLKVAGSNGQATSNVDLNNIRLIKKVGGTIDDTEMIDGVVLTQTSVKNAGGPTRMEKAKIGLIQFQISPPKPDTENNIVVNDYRQMDKILKEERAYLLNICKKIKKSKCNVLLIQKSILRDAVNDLALHFLAKLKIMVIKDIERDEIEFLSKSLNCKPISDVDFFTEDRLGTADLVDEIDSDGSRIVKITGIHQSNNQPQQQEQESFVSELANSVESTLSGNGYSSSKTNYYNNFHTNNTVSVVVRGANNMILDETERSIHDSLCVIRCLVKQPALIAGGGAPEIEASRILMKEARALQGVEAFVWQEFAQALEIIPTTLAENAGLNSIKVVTELRSKHERGEKNAGISVRRSGTTSDAYVEHILQPLLVSTSAITLASECVKSILRIDDITFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RC68</accession>
    <name>A0A1E5RC68_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ERAD-associated E3 ubiquitin-protein ligase component HRD3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2947</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sel-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RC68">
        <property type="interactions" value="314"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RC68"/>
    <dbReference type="InParanoid" id="A0A1E5RC68"/>
    <dbReference type="OrthoDB" id="27934at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050767">
        <property type="entry name" value="Sel1_AlgK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11102:SF160">
        <property type="entry name" value="ERAD-ASSOCIATED E3 UBIQUITIN-PROTEIN LIGASE COMPONENT HRD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11102">
        <property type="entry name" value="SEL-1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184688" evidence="4">
        <location>
            <begin position="23"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="852"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="885"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="887"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="913"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="937"/>
            <end position="949"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ84173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="976" mass="110531" checksum="24B2A6DCB0B4233C" modified="2017-01-18" version="1">MSVRKMLSQSVLLLSLFLAATAMSLGTQNDENAAGNLFNKKHKADSLYQDALVILHNPEYQERQVIMTDSSQLYRSARIETRNTKFLDLPLYKIEQDIFWNGVDNVDAHLIALQMNQTSSENAEKSVIADKQLADVLHTFTEISEPQQFAHDSLVKAALEYHHLDSLYTLAQLHLWNDYGFVGNKSQGLQYLLEFDKVCIENAYPRNVSVLFEIGVAYSTGLFEQKPDHLKALVYYQAAADLGSLHGMQALAYRYYTGFGGVPKDCSKAQMLYSALAEELRFELVNTKTHDMPWDVLPKVNDKYNIRVCDLKEGLIGESELSSVFSKQTKSRLKAKIPDLPGYGLGANAHENPFNQIINNLQNQPLQLNAGRRGGFNVAFGFPTDMNFNVMFGDGNNEYKNDQLIYQYYDALNAYEGTFFFERNVTYAVSVLTGIYKNFDEHVYSEFNEHQKYFYSKALDLLGHIILTAEVFEDVDYDLAKMLLLRAKEIVEFPYLVGTFPSRANVDLGIIAHYHELNFTKAQNYYKQVINTPHDDGTASYNYAKLKLAQRNESPVLSESLDIDNAAIDILIQRAVYKGYDPALYMWSKTKEGRDNGRFRCEDIVLSFKTFTEDLSGVLSPFLKKSFTKYINSDDFGAIWGYAMAAELGYEEAQTSVAYMLASPLLMFEEFPSDTPDLIKNLAISYYVRSYKQNNVDAGLVAGDMYYQQGDYERAAALYQGTATKFSVQGLWNLAYMYEKGLGVEKDYHLAKRYYEQVLEHKPLYMAVKLSVLRLQFLYFWNKYFGAFHSNKLMRAVLTKSLXVLKKISLSIEKIEKLPAWKLSSSIGKKMVRIHPSKILDTLEGYGIDSELLISLITIGLFFLFPLVLTRLAVRNGWNVVINGVPINPPPRPREPAPAPPVRPPELQAPSNENLQQAEQAAVAETASGEGEGXADTSLQXRHTPTASETIIEDVHEPTQATVSEDDDDNDFVVFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RCM4</accession>
    <name>A0A1E5RCM4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">hydroxyacylglutathione hydrolase</fullName>
            <ecNumber evidence="5">3.1.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glyoxalase II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2526</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an S-(2-hydroxyacyl)glutathione + H2O = a 2-hydroxy carboxylate + glutathione + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21864"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58896"/>
            <dbReference type="ChEBI" id="CHEBI:71261"/>
            <dbReference type="EC" id="3.1.2.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.6" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RCM4">
        <property type="interactions" value="491"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RCM4"/>
    <dbReference type="InParanoid" id="A0A1E5RCM4"/>
    <dbReference type="OrthoDB" id="515692at2759"/>
    <dbReference type="UniPathway" id="UPA00619">
        <property type="reaction ID" value="UER00676"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004416">
        <property type="term" value="F:hydroxyacylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07723">
        <property type="entry name" value="hydroxyacylglutathione_hydrolase_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035680">
        <property type="entry name" value="Clx_II_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032282">
        <property type="entry name" value="HAGH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935">
        <property type="entry name" value="BETA LACTAMASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935:SF94">
        <property type="entry name" value="TENZING NORGAY, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16123">
        <property type="entry name" value="HAGH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="10">
        <location>
            <begin position="15"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ84333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="282" mass="31711" checksum="54DC49AA55B99238" modified="2017-01-18" version="1">MQIKALKMRWLTGGVNYSYLVSTSTTPVKSWIIDPAEVPEVVQQLTTQEMSTITNIVNTHHHYDHADGNLGMYEYLKGKVHNXHDIQFIVGSRTPNVPLNDPNTFIVPKDGQTFKLGDDITVKAIRTPCHTQDSVCYYFFDKSTDERAVFTGDTLFTAGCGRFFEGDGAEMDAALNHALIEKVDEPQKTYVYPGHEYTKSNVKFVKKCVYTKPGDNLAFDKLVETVSAKEVTTGLFTLNDELEFNPFMRLNDPLVRKAVGDEGHKKTTSAVMDALRTMKNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDI4</accession>
    <name>A0A1E5RDI4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Nitrogen regulatory protein areA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2386</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDI4"/>
    <dbReference type="InParanoid" id="A0A1E5RDI4"/>
    <dbReference type="OrthoDB" id="515401at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000007">
        <property type="entry name" value="Nitrogen regulatory AreA, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013860">
        <property type="entry name" value="AreA_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039355">
        <property type="entry name" value="Transcription_factor_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071:SF281">
        <property type="entry name" value="BOX A-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08550">
        <property type="entry name" value="GATA_AreA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00619">
        <property type="entry name" value="GATAZNFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="10">
        <location>
            <begin position="619"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="337"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="509"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="670"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="932"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="340"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="358"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="607"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="675"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="692"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="718"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="736"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="754"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="934"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="954"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="980"/>
            <end position="993"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ84959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1008" mass="110121" checksum="DC2A26842178241E" modified="2017-01-18" version="1">MSENFHHQRAGRMEFDPSTDDFVNDMLSNAGQFGENYTTTKTSKSSFNSKTIKADLDNAESLFKIYNDSCSNILPNKERLKNLSWRLLSMNSTHGXLCGKKGFLNANVAGNITQKNEEHAETLKNENLLGAEHGLFSPLNSFHEQLHDGSFSSPDISIQGKAFEKFTTISPGNASASHHHDHGQNIGNDKNIDDLMEVDTQHLDTLSGSHRNLVEXQPSFSHELDYVNQLKTINSETGIDPSYLEPQTTFSTNEAQPSPSTYLNNMDSIIRTGSISNFANAPGQDTNNNNNFDHHRLSTSIADSFLPHSSFSNPMQHVGFLNSSKLSRSMNEHETLIDENGIDDDDDDDEEDNDDESSTPGSATFGLNPNKIISNLSFGVNSMREPGAIDNNNAVGMDQFGRRNLNSSSAMSRSPSVGYSFANNVQTPLLSGSAPLGTEGSFMPSNDKNSNIXGSFSTGASNRISEPIGIKRMNSSNSLLKQKSFTNIHSLPSKNNSFLSTGXITSLSATSQPKDQTEFHSIDFRPNSLPRQGGIRKKQDVIMNSKSRRSSMSTSAGAAVFNHTPTPPPQSAESSSSPLATNRRGPSKAMKAGRATGAKTANKEKNAASNDNSSGSKDPNKDIECTNCHTKTTPLWRRDPMGKPLCNACGLFLKLHGVVRPLSLKSDVIKKRQRNNNTGSTIGAASQDDKVSPXSLNSKSKNNISKASKGKKATDSQGKSSKTSPTSRRGRPPSSNATRSKNQDSTTDIKDENSTTQHTFSFENFPNSSDFFDASMLSQKNSVPLMSHNHDSSTDTIVRDEKIVDKSIRGLPIEESHGIHDDEIVMKSSDVMNATVFDQHELFFGHEQNLASTDHDMSGHLKLSQSYQEHQFIQQRDLAGGTTDVIDIFSGFPNNQDFNSANTDIVSDSISAFSSHKNTGVNNEHITNVXNQPTQLTSSSNSRSPSSSATSHTNFHNGFWNSHNQSQTSNRQTMNESSATQTPVEPPTQTXRTKSAETKNLEWLTLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDT3</accession>
    <name>A0A1E5RDT3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Polynucleotide 5'-hydroxyl-kinase GRC3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Polynucleotide 5'-hydroxyl-kinase grc3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2312</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Clp1 family. NOL9/GRC3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDT3">
        <property type="interactions" value="154"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDT3"/>
    <dbReference type="InParanoid" id="A0A1E5RDT3"/>
    <dbReference type="OrthoDB" id="4054781at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051731">
        <property type="term" value="F:polynucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000448">
        <property type="term" value="P:cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045116">
        <property type="entry name" value="Clp1/Grc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032319">
        <property type="entry name" value="CLP1_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755">
        <property type="entry name" value="CLEAVAGE/POLYADENYLATION FACTOR IA SUBUNIT CLP1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755:SF3">
        <property type="entry name" value="POLYNUCLEOTIDE 5'-HYDROXYL-KINASE NOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16575">
        <property type="entry name" value="CLP1_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Clp1 P-loop" evidence="9">
        <location>
            <begin position="339"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="10">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="770" mass="87554" checksum="8FBE1E3391E9DD68" modified="2017-01-18" version="1">MEENSLLNNHGSLFPVYSNASEDDNSSEMEETDELNVVGAQTTIRSNGKPLLAIEXALVTPALSSNTASEYEDEEEIEEGKEYIQTVEIKEQHKEDQTNSTKTNSIKIVTNTTTPTIRETHINNLKKLNLYTRLEGINYVSTDTYTYIGLQNDEQLFVVGHMGFQIVKGGLIYNTQHFNASPSQTFNVYHPILKAIAPLQSSHYASFQEEVFLSKRELHSLEYANTQKVYHDLDVYKSFANDEDECFKVSDYQTILRVKRDTFNSLNINDVNVLCSKYTNVWKSVENQDYLQDFQILSRQELHNIRTLSIPQDWKQTIQQLLMKYHDPQQTNLRVMIIGGKNSGKSTLNKILLQNFLYSTPNQASSLNYIDLDLGQPEFSTPTCISCAELQTGPVCILGDPLVQRNAFSXDTVKMVKEVFFGSANPNLFPVTYMKSCVELINTMFNYDSVNPTLINLPGWVKGFGIEFLQAVINAYQPTDLVVLESVRQXENNELMNDLQLLSLPAFYSEIEQSTYSXNVSRLRGIFKDADKSLVQNNWHASHIREYKLLTYLHQTQRGYDFTPLLQQTPKRLSFQKPTGLETVKGLIKGFKFSNTFGVGNKVTDKDISTIINCLQGSVTGLHSVKLQEESSVSKGGENTPLIQLNPFQKKFPIVEHFHKDAEIKLLALCVIHSVDLENGFVNFFVPERYHSEIESVVQSKXXDLVMVKSSMELPACELIPENGFLVQYMKSANSIDLPPFISSNKXRKLNDHVWKVRSNILRRGQTXEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDZ8</accession>
    <name>A0A1E5RDZ8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative histone deacetylase complex subunit cti6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1942</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDZ8">
        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDZ8"/>
    <dbReference type="InParanoid" id="A0A1E5RDZ8"/>
    <dbReference type="OrthoDB" id="418595at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061188">
        <property type="term" value="P:negative regulation of rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061186">
        <property type="term" value="P:negative regulation of silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053051">
        <property type="entry name" value="HDAC_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47793">
        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT CTI6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47793:SF1">
        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT CTI6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="325"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="22"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="131"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="170"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="400"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="452"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="131"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="189"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="216"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="240"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="297"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="498"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="543"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="591"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="616"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="701"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="720"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="7">
        <location>
            <position position="411"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="7">
        <location>
            <position position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="834" mass="93882" checksum="B52ECC0B0D4DD59B" modified="2017-01-18" version="1">MNDSHANTINELTAVSPVINTNTINPESVNXTNDIANTDNQNTSSGNGNSNAKLDDRSXXXVVDETHQYLETNATNTDNIASILPNNNITDSEIVNNDSLTQTNTNDPAIEKNETTVSTAENDDKDQINTLNVASSSSNGDDATKEVQHTETNGEYDTLQEQANDAIKENHEGELNSPKSNDXNXNDSGTHDKMNDQKLEQGENNELPKNTVEVPTKNSINSDNEQGNNLQKEGSXLLLHENDGTSERFDQSRIGEIEPFREEKSDHEHEKEEELEDGQREKEAKPQREEXEKEXXEAGEGEEEGEGVGEGAGEGEGEEEEEEGETRCVCGELDPPDERGAYIQCDKCSTWQHQFCVGFKNESELPEKYWCENCMPSFHTLYLNKKLNLINSRYEPLMLXKTKGSPPSXNDXSLENHRSTRRSARYNDRNSDKEDDIDLEQLKNKKYXKQILSXNDDNEGENDQNQSAKNLKRKHXASNSNSPSKKIRKKGGFEVTADTRSNFRSRATSLAREEVQYQMMLEKAIEESKKSSSNHSSPRMKTDEEENGADGSEGENEENLLQTKKLLNGVNAKLESDQQANSEEANEGFIXXXNDXLSKNSKNAGSHVVERHTENETEEGADGDEELDIEEDNDEEIEEEEGDIEEEDDEDGNHNEAGRFVKPKRPSRNTDGRALXRSKRLRSTKSAPNSPSVRRSKSKRAASSSSSPSASSTNLSSKYRTNEKSFTNKDNKTSNKKNGKEEISLDKPSKPRLPTPNVTLQEMHKRVGAIMEFVSRTKKEIEDLQSQDPELLRYVENQEFIEKFKEFNNIEILNRMDVLTEKLLQWKENFASDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE14</accession>
    <name>A0A1E5RE14_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Tyrosyl-DNA phosphodiesterase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1944</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tyrosyl-DNA phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE14">
        <property type="interactions" value="470"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE14"/>
    <dbReference type="InParanoid" id="A0A1E5RE14"/>
    <dbReference type="OrthoDB" id="47785at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017005">
        <property type="term" value="F:3'-tyrosyl-DNA phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010347">
        <property type="entry name" value="Tdp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12415">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12415:SF0">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06087">
        <property type="entry name" value="Tyr-DNA_phospho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="54"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="225"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="480"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="482"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="11">
        <location>
            <position position="519"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="13">
        <location>
            <position position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR610347-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR610347-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR610347-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ85116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="620" mass="71774" checksum="BFCA9AC66BF4627F" modified="2017-01-18" version="1">MSKQERNAFEDVKSHWANKKLKANPPETVSYTENPENDTQRVKNISKPTIIELSSDDDDDDDDDNDDNDGAGCTIVGSSQTRKPPIDTTQRSQTHIQNMVYPRMYLLYSPLYDPLSCINNANKAVNPFYXTEQSMFSTLATELQSDKLYHRETLFLWSFQFDFEALIPHLELHNTRVSRVIYXFHQQGTVLPPEDPVLLEVFEDLVRKRLLVFRQHQMPAYSSFHCKMILKVNHQLNKCKIIIPSSNFTKLEPKYVQQMVFDSGMMALETDTNERDLPNESSHNFKNNFIQFLKIFDEKFKNDHVISEILRIMNCKADFTFLNEKYDFVFSSSKNEQTGLQLLKKTICAKTRDCKTSLINTPKTKFIVQTSSIGAPLSQYGDSTLLETSFPQCLPILLNQKTKYEIVFPTSQSILNSPLGLLTSGWFHFHHKRSSKIASMYRRWKQSRMLFTHSHGYRTGNSSHSDATVSSTTRQYVPSHTKFYIVEDLDDIETFKENNSNKNTGKTVQMWMFTTANLSKAAWGVYHLKPVNGIHKFTPPTNFECGVLRVCKEKNSKNKITLRNIIEHQQRWNNTPEVDKPAFRDFKKEYEDGVVYCIFDPSKLVPYDDTDIAFCPEESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REB0</accession>
    <name>A0A1E5REB0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Essential nuclear protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2054</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bystin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REB0"/>
    <dbReference type="FunCoup" id="A0A1E5REB0">
        <property type="interactions" value="889"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REB0"/>
    <dbReference type="InParanoid" id="A0A1E5REB0"/>
    <dbReference type="OrthoDB" id="2192561at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007955">
        <property type="entry name" value="Bystin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12821">
        <property type="entry name" value="BYSTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12821:SF0">
        <property type="entry name" value="BYSTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05291">
        <property type="entry name" value="Bystin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="95"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="481" mass="55016" checksum="1B72AAE3B4DC5644" modified="2017-01-18" version="1">MGRVSAKKQRRHDPLMTDLTSHEGHFKEAPRSSKKQNAANDNENDDENDAPEYIDARQSRKILQLAKEQQQELEDDEEEKQEQLSSISKFRVRDENDDDDDEEDEDNEVNSAIASDAESAGPVENEEYEEEEEVIEINEDDAAMFDSYFKPSSAFNSASGAYNLADKIMAAIREKEMGLATDLAGTDAPFMAQVEEDRGEGVALPPKLIKAYTTIGTILTTWTHGKLPKLFKIIPTLKNWQDVLYVTDPERWTPHVCYEATKLFVSNLGAREAAKFVNMVMLPRFQYEVEENEKHDVNYHIYRALKKSLYKPSAFFKGFLFPLVEYGCNVREAMIAGSILQKVSVPVLHSSAALAWLVQQKYSPAAVVFIRILLEKKYALPYQTVDECVFYFMRFRNEIDGREVNGVVTVLPIAWHKAFLAFAQRYKNDITEDQKEFLLETIRQRPHPEISKEIRRELLAGKSREFVESTTGPANDLMLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RED2</accession>
    <name>A0A1E5RED2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">V-type proton ATPase proteolipid subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g1856</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Proton-conducting pore forming of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Proton-conducting pore forming subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11 12">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d, e, f and VOA1). The decameric c-ring forms the proton-conducting pore, and is composed of eight proteolipid subunits c, one subunit c' and one subunit c''.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Vacuole membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RED2"/>
    <dbReference type="FunCoup" id="A0A1E5RED2">
        <property type="interactions" value="156"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RED2"/>
    <dbReference type="InParanoid" id="A0A1E5RED2"/>
    <dbReference type="OrthoDB" id="1744869at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18175">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18176">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.610:FF:000003">
        <property type="entry name" value="V-type proton ATPase proteolipid subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000245">
        <property type="entry name" value="ATPase_proteolipid_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011555">
        <property type="entry name" value="ATPase_proteolipid_su_C_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01100">
        <property type="entry name" value="V_ATP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10263">
        <property type="entry name" value="V-TYPE PROTON ATPASE PROTEOLIPID SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00122">
        <property type="entry name" value="VACATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <keyword evidence="4 12" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="96"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="133"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="13">
        <location>
            <begin position="22"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="13">
        <location>
            <begin position="100"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ85250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="165" mass="17159" checksum="E37527AB2300FC03" modified="2017-01-18" version="1">MSSIVEAVNYAPDYAPMIGFSGCFFAMCLSCFGAAVGTAKSGIGIAGIATFKPELIMKSLIPVVMSGILAIYGLVVAVLIAGGLNPVETYTLFNGFMHMSCGLCVGFACLSSGYAIGIVGDVGVRKFMHQPRLFVGIVLILIFAEVLGLYGMIIALILNTRGSGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RED5</accession>
    <name>A0A1E5RED5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">RuvB-like helicase</fullName>
            <ecNumber evidence="15">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g1855</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA helicase participates in several chromatin remodeling complexes, including the SWR1 and the INO80 complexes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the RuvB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="15"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RED5">
        <property type="interactions" value="1607"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RED5"/>
    <dbReference type="InParanoid" id="A0A1E5RED5"/>
    <dbReference type="OrthoDB" id="10060499at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097255">
        <property type="term" value="C:R2TP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002221">
        <property type="entry name" value="RuvB-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000010">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.360:FF:000002">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.360">
        <property type="entry name" value="RuvB-like helicase, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027238">
        <property type="entry name" value="RuvB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041048">
        <property type="entry name" value="RuvB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042487">
        <property type="entry name" value="RuvBL1/2_DNA/RNA_bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010339">
        <property type="entry name" value="TIP49_P-loop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11093">
        <property type="entry name" value="RUVB-RELATED REPTIN AND PONTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06068">
        <property type="entry name" value="TIP49"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17856">
        <property type="entry name" value="TIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 15" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 15" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="68"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ85255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="491" mass="53429" checksum="271E4C38E151EB2A" modified="2017-01-18" version="1">MSIQTLSNNDVSSNQSLALIAAHSHITGLGLDPETLQPKESSQGMVGQIQARKAMGVVYKMIKMGKIAGKCVLVAGPPSSGKTALAKGLAQELSNANSDIVDLSSGAGNGSISGDNXKNKVPFTAIAGSEIFSLELSKTEALTQVFRKSIGIRIKEETELICGEVVEIQIDRSITGGHKQGKLTIKTTDMETIYELGNKMIDGLTKEKVIAGDVITIDKASGKITKLGRSFARSRDYDAMGSETKFVACPEGELQKRETVVHTVSLHDIDVINSRTQGVLALFSGDTGEISSEVRDQINTKVAEWKEEGKAEIVPGVLFIDEVHMLDIECFSFINRALEDEFAPIVIMATNRGVSRTRGTNYKSPHGVPLDLLDRSIIVATTLYEEKEIQTILKIRAQEEEVELNDDALALLTKIGSETSLRYASNLISVSNQISLKRKQNSVEVKDVERAYLLFLDSARSVQFVTSNKDQYIDDQGNVEISTEDKMDVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RED8</accession>
    <name>A0A1E5RED8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein kinase-like protein SCY1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1921</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RED8">
        <property type="interactions" value="836"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RED8"/>
    <dbReference type="OrthoDB" id="79687at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051177">
        <property type="entry name" value="CIK-Related_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984:SF6">
        <property type="entry name" value="SCY1-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984">
        <property type="entry name" value="SCY1-RELATED S/T PROTEIN KINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="706"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="798"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="720"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="801"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="843"/>
            <end position="862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="862" mass="97536" checksum="7A70B20B6F8575F6" modified="2017-01-18" version="1">MLGGFFGGKNGINANYTFSSTPTFSSEPWTVYTGKPKSSSKYTNFAKCSIFVFDKKKYEQYMTKNGLVTSSNKEYIHEAYALLKKQVSNLTKLKHPNILQIIEPLEEHSKNFLFVTEYVSGSVDAVYRKDNYNGMYNSTHQQNLSSGNILDDGVNGENSTNNDVLFQRGLLQIAKGLDFIHNTAKSVLLDLQPNNVFINENGDWKLGGLMHLCKLPTGSDNSDFLLDTSAESHPRLPSFLHIPYNYTAPELVFDNHLSCKNDYFSLGCLIYFLYYGDALFNCENSAHYYKQEYGKFEKKLTQLGWDRIFPKITNQKLLVLLPKLMNRDLYARYDNIQDLYDQDFCKDPLITTLVFLDDFPTKKIDEKKAFLQGLIEYLPKFPKQISQKSILPHLIDVLXLGYNVKSDHAELIPVVLELILIIGSQFSQLSFNEKIYSHLRIQELIANNASIVLFKYLSVLQEKVKKDDFVDKILKPLLTATMDISNTNNNNNNNNNNTTASNLIETQEWCLKTEIVDLVLLTFDFITIKNFYFPLISKTFIMTTSLNIKISCAKCFHSMISNKSIEKYQAMDDIMPLFAGMKTRDQKMLLTCLPIFVKISDLYMEEEAVIVQKILPLVWNFSMADTLSLSNYKKYNQVINTISLKVQQNHEAKVQKIESRNISVSDKSESEIFENMINQTASEMTAAKTDTDRLESKRIVSVAPIKPVSKKTTPKARPSDNGQSQVNFVANTGTNESQKQTKRTPMVALNPKPKPRTVPVEKSSYNYFKDDSSSSTVVATDDFDDFVQAKPSVQTNQTRFTAASTVPATPSTLHRPLTSTTSSPLQPSSKASTPQPPPGFSMSLLQPSARNSKNQSNDSSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REG0</accession>
    <name>A0A1E5REG0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REG0">
        <property type="interactions" value="859"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5REG0"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="unsure residue" description="D or N" evidence="1">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ85291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="470" mass="52800" checksum="CBE34A3FC99B8D2D" modified="2017-01-18" version="1">MSILAISVEETNRIRQYFGLKLLPLLNSDKDYKKDKSHGSKQKKISXKDGNDLLGNYDASNFIDTSLFKKNKLKPHNKGTGLKQRRIDEDDXNDVAXSSDWLGKLAEKETQIPXIDEXKSVKSNTAKIKSSLANNIIMTLKEQDILGXQNDSSNPKNVLTVEKEFETKNFADVSQSSKFRKRKIDSKQNEELQAAKKNKKVKIEGKVRVSFGSLDXSADSNGYEHEPSDYMPAKIKKRKAKMLSQKPKSSLPHFEIKKVDLIDDDDPEEENGALDEIISSSRIQNVSTPYKVXRKDSTXNVHLRNDNPDKVQYEDHSEFFNLLKAPXLETGNLGHSHADVETEKLTDXATDEKIATNNKTVEKSISEEPNFYDGMASTLQFLSNSVSTASNSNXEGPVASIPLPANSTXSSLSKTNVDVXTTKEKKPLFSINLKYTDDVTGEDLDPKKAFRQFNKNYYKAKKKPRSAKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REM9</accession>
    <name>A0A1E5REM9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1679</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REM9"/>
    <dbReference type="InParanoid" id="A0A1E5REM9"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="204" mass="22653" checksum="0C2E65EE40B8A461" modified="2017-01-18" version="1">MRGAKAALKRKLHKSKAALKCKLHKSKSELKCKLHKSKTELKFKFADRGLFKACGKSAQAYEFACKRVQPAESLRVGDSLESRGSNTNDADPNSETALPVSSTSASDYGAVPNDGFSEQETLGNKKAVKRSKRARIRDAKAAFKCNVQHSKARFKCKLSKCSFLKVNKDHYQANEDSNERGRPDELLREIDTLTSNGVDIDMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REN5</accession>
    <name>A0A1E5REN5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ras GTPase-activating-like protein IQG1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1847</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REN5">
        <property type="interactions" value="382"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REN5"/>
    <dbReference type="InParanoid" id="A0A1E5REN5"/>
    <dbReference type="OrthoDB" id="775356at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046580">
        <property type="term" value="P:negative regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12206">
        <property type="entry name" value="RasGAP_IQGAP_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000593">
        <property type="entry name" value="RasGAP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14149:SF14">
        <property type="entry name" value="CALPONIN-HOMOLOGY (CH) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14149">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN WITH IQ MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03836">
        <property type="entry name" value="RasGAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="3">
        <location>
            <begin position="176"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GAP" evidence="2">
        <location>
            <begin position="1086"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="104"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1659" mass="188067" checksum="B9214B0D04A4C3A5" modified="2017-01-18" version="1">MAATLMGSPEKSHSALKQRYLQSLQNTGMSLEPLKPSSSTRISSLSGSPKRHYSSVLHKSPVKNDLHKLKQEPAFVNQNSIKPSSVLNSRKLFELSSSKEPLESQPSPKLKQQKQVMSKNASIKSPSKATEMPDLNNFSSKSNIDHARSVTKISSNFEKVDTKKYSPIELKYYEFLCRVYEAKEWMESIIDEELPSVMSLVTRNALQDGVALAKIAQKVNSSLVPQVHPSTTKFKYKLLENIENFLLLVDTVDVPNNFKFESTDLYDGKNIPQVFETLHALAFTLHQKWPKKVPAMINEFGLVNFSKDDLRDCKIKFPHLKNFQFFGHDHHSPNKKKPQNHEQDLIPNLDDVDRQISXKAQDTQNDEQLQESRITETSDLPFAKINPTKSYSAFNSKYTKELALDDDLEKSLKLSQTIDKMDDNRISSVVVKNPLPVELLVEKDTNDLEASLSSSFNYSSKLKDIPVINYAPRQDLSYYSPSISAQISPRRRRRRRRANMYSAENQFDSSLSSSGSEYSNPFSSTGSYAASDQYSYSEYQPSSVHEYDLLFPKKRTGHAAQNTHSAQNADLISKVQGICRGSNTRYALYALDLKLEFFEEEITAIQACLKSKILLQSIKSKLQIVAASNESHSLAQFQAYIKGSQIRFKYDRRMFDLSRHHLSVDAFQALTRGYAVRQKSSKLQNSIVEASVSIQKLQSLIKGGNARLEFNQITNFLTLNIASIVKSQAIVKAGIARKKINAQMNCCAASRSTQRSIILLQAYVKGSLHRKNAGRTERSLNKSRNKSALLIGCIRGAIFRDSFYETLYNIENFKEEASSLQAQLKGVLARYAVQLLDNVIDTHSVSLLQSHVRGGLKRIKIKQTSDYYNGNVKAVITLQSRIRSFALEKAYHELMDFANPSLWALKKFAFLLNGVQPTYETQDTLDNLKEAIDKKNIAINKSDLKLRNTGEKLSVLKSSGVIKHSLNPEVGNILQTLKGSVNFENCETIAYFEKIFYLLQVDPFYFRLLFSVEPKTVKKFLILLFFKRDGSISSREGLLYMKLVSELLLVDVESRAHVSDFLLEQFDDEPWKQLLNIFLLAKHKNLMNSLFSGLIKALDSLDVSFEGNPSKIYQSLYGLDKDVDPKEAIEDKATNAQYITNMCTLWSFIEQVHGIILSNIDNLPIEVLHLCTKAYTAVAGKSSDEFDALEAISKVIIGAFVNSYLLQFDAYGVQLQHTNHTVANLEILSDALSTVFAMRKFKGFYSQLNSYVEAIGNEIAATLQHLQITPAYEEQMESMVYEDMNKCSRPILTINIKYLSEIVDFIYQNIEMLPQDDPLVELLETVQDLGNTSLFTRSSVVNLELNPSAYILCDVSDRGRPVFNNVMWGLIYVIQICSAHCSSVLDVLSKPEEEDEDVLEMRFQNLLSKYPSLKENAVFKELPSVSYFELKMYVTEKTLELSNMSFIDRSGNFQCLLNDIANTIKCHVYVKTRNMKEIDIAERTLGMLETKHQKISHEYQLLKQSFDTSLGGVKPVVHFNAKKKGLGSKLKGMVHTKNNKDLSSTRYKWSLRQLYEKGILLGIEGEKLESLPVNYFGGGGAKFPNVDFVFSTSNGKEYIIEMIDNRKNHGSARTSDRFNFESVLERQMNSSAKEMMLANNKAKFNTKKLFKMIVESFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RET4</accession>
    <name>A0A1E5RET4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NADPH dehydrogenase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2457</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RET4">
        <property type="interactions" value="869"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RET4"/>
    <dbReference type="InParanoid" id="A0A1E5RET4"/>
    <dbReference type="OrthoDB" id="3969599at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02933">
        <property type="entry name" value="OYE_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000138">
        <property type="entry name" value="NADPH dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045247">
        <property type="entry name" value="Oye-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893">
        <property type="entry name" value="NADH OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893:SF91">
        <property type="entry name" value="NADPH DEHYDROGENASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="4">
        <location>
            <begin position="19"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="396" mass="44449" checksum="FE2AB669DBE633AC" modified="2017-01-18" version="1">MTYTYVKNYTPTDFIGSNLFKSIKVGNMELPQRIAMAPLTRMRADDSQVPNLDNMAEEYYDQRSQRPGTLIITEATFISPQASGYPNAPGIWNDAQATQWKRIFERIHKNNSFVYVQLWNLGRQASPAYLKSQNLKYVGATDDLYMDESSKKEALDSDNELKGLTKEEIKAMIQDYVKAAKLAIENGADGVEIHSANGYLLNQFMDPISNQRKDEYGSSIENRSRFTLEVVDAVVAAIGADKVGIRLSPFGQFGAMSGGDNPLIVAQFSHVLGELEKRAQENTKNRLAYVHLVEPRVTDPFAAEGEKEYLGATNDFAYSIWKGVVMRAGNYVLDNKTALKDVNENDRTLLALGRFFISNPDLIDRIEKKLPVTAYVRETFYAKGPVGYTDYSXYSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RET7</accession>
    <name>A0A1E5RET7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1822</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RET7"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="98"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="282" mass="31589" checksum="BD269320A28BF246" modified="2017-01-18" version="1">MNPFGSXGTAKQQRVSLFKKQKXAHGDTIIIVSXFKLKXFAPPPTCXENVSETTSNAPATPATPATPDNPFTLATFIAVFRARQLEFAKAYLFGNKSISTVERMLDNHKGELXKHEEKLASGKLRPLLLTDCRFAHTHGTDTTSSFVPKTPCEWACVYNQADHQLARIRCKRTMXEIERSREKAVANGAVDNAKIAEIWSQGVSRCTKEISDKADAMVKFLQPNVSERLDLDCYIRNMKNPTPSSLPDNVFADEEYRLLLTKIHFIEYVIAEKQKAMDSNPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF82</accession>
    <name>A0A1E5RF82_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor eIF2B subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">eIF2B GDP-GTP exchange factor subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1981</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF82"/>
    <dbReference type="FunCoup" id="A0A1E5RF82">
        <property type="interactions" value="1078"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF82"/>
    <dbReference type="InParanoid" id="A0A1E5RF82"/>
    <dbReference type="OrthoDB" id="269919at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051855">
        <property type="entry name" value="eIF2B_beta_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45859">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45859:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="488" mass="53574" checksum="380724540F8B142D" modified="2017-01-18" version="1">MRRQNHNLKMSNNVEPGTEPSEIDVLINPFIEKLKTRKIQGSYEISMQTLQLLKRFIGAVRFSSHEELFTKLSNLGYKLEKAQPREFGSGNMIRRLLYVLKEELLTANIQHGFSNNPGAIGQTGTLMQHQNNSAMYDSNKEHKNEPMISSMFSLLQTPHPSELTSFKNGTLRASTPITTAANANHTAFAIGTSATVPLDEELSANAAAQLPAAFVASTTSGAAEDPAASGHHADARSKKPSKFFDIRATLINAIKDLMDEIKNIDEGIFSIAIDLIHDREILLTPTPDSKTVLKFLLKAKEEGYGKVRNFTVLVTEGFPNNVQNAQDFAWKLSSHGIDTVIIPDSMVFSVMSRVGKVILGCKQVFSNGACIVSNTGVTSCCECAKEFKTPVFAVAGLYKLSPLSPFNITDYIEVGGSNTGKLLPSGNSNSTDKTDYTRLTMINPMDDYVQPENIDIVITNIGGFAPSFMYRVVLDNYKVEDVNFKPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFD4</accession>
    <name>A0A1E5RFD4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Serine/threonine-protein phosphatase 2A regulatory subunit delta isoform</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2262</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFD4">
        <property type="interactions" value="691"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFD4"/>
    <dbReference type="InParanoid" id="A0A1E5RFD4"/>
    <dbReference type="OrthoDB" id="10264446at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000372">
        <property type="entry name" value="Serine/threonine protein phosphatase 2A regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002554">
        <property type="entry name" value="PP2A_B56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE 2A PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A 56 KDA REGULATORY SUBUNIT GAMMA ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01603">
        <property type="entry name" value="B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="322"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="720"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="115"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="145"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="187"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="724"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="769"/>
            <end position="778"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="907" mass="102526" checksum="8CC341952F4AD9C7" modified="2017-01-18" version="1">MMRGFKQKLIKKTTGGSSSSSTNNNTGSGNNSNSNSNNTNTDKNSNXNSNNNNNSXSNKSSGSSILKNGLLNNNNNNNNNNNXNHNSSXSTSNSSTSNKKKSSDSSQDTAKSRKTSGLNGTNTTTNTGHKHHHENKNGDLKTTHHSXGGNNNSTSSSSHSTNSSNNSSSNNNTTGHATLLKAAVVTTPKQPQQPQVVTPHTHTHGHNINPPNNNNNNNSNNNSTNNNNNNNNNNNNSNNXNNNNNNNXNNNNNASSSXANSNINTSSSTSSTPDVNMDIPPSSPSIDIPRSSHSFEKLPTXTKTNLLQHTGDTDMDLINYNNNSSGNGVNKTPTRKTSSRFDHSRYQQLSKLPNFDELNDNGEGMNGSNKEQYIQLFLAKIDQCKVMFDFTDPSFDIQGKEIKRCTLQELIEFIVTHRFQYPDVMYKEIVDMFKINLFRPIPPPVNPQGEIFDPDEDEPVNELAWPHMQLVYEFFLRFVESPDFNHSIAKQYIDQNFIIKLLELFDSEDIRERDCLKTTLHRIYGKFLSLRSFIRRSISNIFLQFVYETEKFNGIAELLEILGSIINGFALPLKEEHKIFLIRVLIPLHKVRCLSLYHPQLAYCIVQFLEKEPMLTEEVIMGLLRYWPKINSSKEIMFLNELEDIFEVIEPLEFLKIEVPLSVQLTKCISSPHFQVAEKVLSYWNNEYFLNLVIENSEVILPILFPSLYELTCQLKLDNGINTDLDDDNDNDMILDDEEDXEKXDGGKSENANTQDKAQKTKNNKNENDTNDTNDTDTNESTTNGLSESLHGRKADKLKTMEDINDLLMNDQDPYLLVEQAINSGSWNRAIHAMAFKALKIFLETNYPIYEQCQKMYLEHCKENSVLINSSKNMNGITASIELRNKKRLENWDKLEEIVHEKLKINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFD7</accession>
    <name>A0A1E5RFD7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Protein ROT1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1745</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Required for normal levels of the cell wall 1,6-beta-glucan. Involved in a protein folding machinery chaperoning proteins acting in various physiological processes including cell wall synthesis and lysis of autophagic bodies.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ROT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFD7"/>
    <dbReference type="FunCoup" id="A0A1E5RFD7">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFD7"/>
    <dbReference type="InParanoid" id="A0A1E5RFD7"/>
    <dbReference type="OrthoDB" id="5327821at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006458">
        <property type="term" value="P:'de novo' protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007118">
        <property type="term" value="P:budding cell apical bud growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019623">
        <property type="entry name" value="Rot1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28090">
        <property type="entry name" value="PROTEIN ROT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28090:SF1">
        <property type="entry name" value="PROTEIN ROT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10681">
        <property type="entry name" value="Rot1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017290">
        <property type="entry name" value="ROT1_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184727" description="Protein ROT1" evidence="11">
        <location>
            <begin position="23"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR017290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ85605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="285" mass="32029" checksum="FB3D09743F5E01C7" modified="2017-01-18" version="1">MRLSSLPCFSALFIAFALSVSAQIEELEIQEIEMKDTTTATTSSNQDLIDKLQGTWSSKSNQVFTGPGFFDPVDELIFEPSLPGISYSFTSDGFFEEARYQVVPNPQDPSCPSASIIFQHGTYSFASGNNSLMLYPFEVDGRQLVSDPCNDNGVSSYTRYHNIEVFKWFLVEFDTYHGCMRLDLYEYDGSPVQPLYLAYDPPIMLPTVTLNPTDSADSSSQKRKRDFNGSKLSLREKLKKQLENKYKTNAKIPIQNNIFGLENHAVLVWGSMISLFVGSSLFLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFN8</accession>
    <name>A0A1E5RFN8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ATP-dependent RNA helicase DBP9</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">ATP-dependent RNA helicase dbp9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AWRI3579_g1993</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits and is required for the normal formation of 25S and 5.8S rRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DEAD box helicase family. DDX56/DBP9 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFN8">
        <property type="interactions" value="1017"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFN8"/>
    <dbReference type="InParanoid" id="A0A1E5RFN8"/>
    <dbReference type="OrthoDB" id="1191041at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17961">
        <property type="entry name" value="DEADc_DDX56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF21">
        <property type="entry name" value="DEAD-BOX HELICASE 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 20" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="19">
        <location>
            <begin position="17"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="49"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="254"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="348"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="560"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="15">
        <location>
            <begin position="17"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="357"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="569"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="587"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OEJ85687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="596" mass="68545" checksum="9479E059832301A0" modified="2017-01-18" version="1">MTENSNHVSDAYLDESLTFDSFKLDSRLLQSLKSNGYHHPTLIQSSAIPLAIQDKKDIIAKASTGSGKTLAYLIPVLQTILEFKKTNXDHSLQRDGSSSMGIILVPTRELSEQVYQVLQKIVTYCSQDIKSVNLSSSTVSDNVLKSLLMESPDVIISTPSRLVQVLDNNNSQRSNFTLEDLKFLVIDEVDLVLTFGYQEDLWKISEYLPLKKNLQTFLMSATLNDDIQNLKEKFCRSPAVLKLNDEEINKDQNKLAQYYVKVTEFDKFLLCYVIFKLNLIKGKTLIFVNNIDRGYRLKLVLEQFGIRSCILNSELPANSRQHIVEEFNKNVYQLLIATDDTEYIREEDEDQLEDISDXEKEKQGKDKNXSXDKESKIVKDKEYGVSRGVDFKNVSCVLNFDLPTTAKSYVHRVGRTARSGKSGTAISFVVPLKDFGKHRPSSCPTCKKDEKILSRIIKQQSKLGFEIQPYAFDISQMEGFRYRMEDGFRAVTQVAVREARIKELKQELLTSEKLKRHFEENPQDLQSLRHDKELHPARVQQHLKRVPEYLLPENARQDKKKISFVPFHNSKKKRVHKKKGNNNRRQGKTDALKNFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGD1</accession>
    <name>A0A1E5RGD1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Methylenetetrahydrofolate reductase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1770</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methylenetetrahydrofolate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGD1">
        <property type="interactions" value="426"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGD1"/>
    <dbReference type="InParanoid" id="A0A1E5RGD1"/>
    <dbReference type="OrthoDB" id="16284at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004489">
        <property type="term" value="F:methylenetetrahydrofolate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00537">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004621">
        <property type="entry name" value="Fadh2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003171">
        <property type="entry name" value="Mehydrof_redctse-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053806">
        <property type="entry name" value="MTHFR_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00677">
        <property type="entry name" value="fadh2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45754">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45754:SF3">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE REDUCTASE (NADPH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02219">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21895">
        <property type="entry name" value="MTHFR_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="FAD-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MTHFR SAM-binding regulatory" evidence="9">
        <location>
            <begin position="352"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="MTHFR SAM-binding regulatory" evidence="9">
        <location>
            <begin position="602"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="552"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="559"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="703" mass="79774" checksum="853F2A90661E69F3" modified="2017-01-18" version="1">MRISEKLQKHKEKYPDKPTFSYEYFVPKTSQGAQNLYDRMDRMYELTGPQFIDITWNAGGKKVEKKPIVQNGXSTNGSSTKTNEXVSSFNGFRADAKPTTTDIIETCQTVLGLETCMHLTCTNMPLSEIDEALQKAYDSGCQNILALRGDPPLNGGDNGDDFTGDGDDASCFQYAKDLVKYIREKYGDHFDIGVAAYPEGHPEESNRELLLDFLYEKVECGADFMITQMFYDADIFIDWCAKVRARGITIPIIPGIMPITSYDAFLRRAKWCQINIPKQFLKELDPYQEDDQMVREIGTKLVVEMCMKLLQSKYVTHLHMYTMNLEKGSMVILQKIGALRKNQNTLSSNQLWRRSLNPQRKNEAVRPIFWQRRPYSYVARTSQWGADEFPNGRFGDPNSPAFGGLDLAGAQLIRQTGKRCLELWSQPTKVKDISDLIINYLNGDLKCLPWSDTPITGEVDSILHYLKNLNKKSIFTINSQPKVNGLPSSDKIFGWGPKNGFVYQKGYLEFLLPKDKINQFLELANNNEELTFFLLNNDNSIFYHKDGENGTVNESVDYNSETSTNSTTAENGDVSPSSSSKSSSLSPIGQTENGNKKRKVERKKSCDTDNLSVSRANAVTWGIFPGREVLQPTIVEKESFVAWRDEFYQILQEWKLNFHKFNAPESATLIGKIIDDYVLCNIVDNNFVSPENQIYSLLSELGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGG0</accession>
    <name>A0A1E5RGG0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Apurinic-apyrimidinic endonuclease 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1370</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AP endonuclease 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ85998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGG0">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGG0"/>
    <dbReference type="InParanoid" id="A0A1E5RGG0"/>
    <dbReference type="OrthoDB" id="7663182at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00019">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.150:FF:000001">
        <property type="entry name" value="Probable endonuclease 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00152">
        <property type="entry name" value="Nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018246">
        <property type="entry name" value="AP_endonuc_F2_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00587">
        <property type="entry name" value="nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445:SF0">
        <property type="entry name" value="APURINIC-APYRIMIDINIC ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445">
        <property type="entry name" value="ENDONUCLEASE IV ENDODEOXYRIBONUCLEASE IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00730">
        <property type="entry name" value="AP_NUCLEASE_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51432">
        <property type="entry name" value="AP_NUCLEASE_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Xylose isomerase-like TIM barrel" evidence="10">
        <location>
            <begin position="33"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="297"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="326"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="11">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="359" mass="40715" checksum="6FB4B54273AD9C43" modified="2017-01-18" version="1">MKKTFERSATSKFKFGAHVSGSGGISNSVINAFNIGCNAFAMFLKSPRKWTAADYTAEEITKFHNNCKEHHYDPKTDILPHGQYMINLANPELDKAEKSYESFLDELKRCEQLNIGHYNFHPGSSLKGNHDKQLEQLASYINKAHKETXFVKIVLENMAGTGNLVGSDLHDLKTVIEMVHDKSRVGVCIDTCHAFAAGYDMVGNYDKFWQDFEKIIGFDYLXGIHLNDSKAPLKANRDLHEQLGQGFLGLKFFEKIAHSQFLHDVPIILETPHKDEEGYGEEIKILEWLETLDESSAPDNEQVIEKSKTLQKLGEPSRKKQQQAFEQKKLKSEKPSKKRKXSAKDGQDIMSKLTKKSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGJ8</accession>
    <name>A0A1E5RGJ8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cell wall protein CWP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1404</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGJ8"/>
    <dbReference type="FunCoup" id="A0A1E5RGJ8">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGJ8"/>
    <dbReference type="InParanoid" id="A0A1E5RGJ8"/>
    <dbReference type="OrthoDB" id="5415592at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184743" evidence="2">
        <location>
            <begin position="22"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="155"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="246" mass="24900" checksum="D15B785ECD4074C1" modified="2017-01-18" version="1">MKFTSTIALTSLMAFFSTCLADSESFGLISLRSASAVHLSSVGPSDGVVVVGYQGNSISAVVTDEGYLKFSDGSYIAVTSEGQYTVTTDSSKATSGWSLNDGYLKINNAQSYVAVPVSGTYDLYNQGSASEDIGISIGAFTSGGSRAADFTPSGSNSDSNSDSNSDSNSNSTVTTVAPTYSNGTTTVSSYSTTLISSEYCGGKVCNGTNGGNSTTITSQSENIGLKMNGGNAGALGLFAAIGALLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGK6</accession>
    <name>A0A1E5RGK6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Altered inheritance of mitochondria protein 18, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1151</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AIM18/AIM46 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGK6"/>
    <dbReference type="FunCoup" id="A0A1E5RGK6">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGK6"/>
    <dbReference type="InParanoid" id="A0A1E5RGK6"/>
    <dbReference type="OrthoDB" id="18193at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016872">
        <property type="term" value="F:intramolecular lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016087">
        <property type="entry name" value="Chalcone_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016088">
        <property type="entry name" value="Chalcone_isomerase_3-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036298">
        <property type="entry name" value="Chalcone_isomerase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47284">
        <property type="entry name" value="FATTY-ACID-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47284:SF3">
        <property type="entry name" value="FATTY-ACID-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16035">
        <property type="entry name" value="Chalcone_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54626">
        <property type="entry name" value="Chalcone isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Chalcone isomerase" evidence="6">
        <location>
            <begin position="92"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="297" mass="32854" checksum="86D41F2CABB714B0" modified="2017-01-18" version="1">MFKTRLFSTASRAAKDTFFPKHLFKGASLVCGGTLFAGAVAYNSYNFQSVAGQLSNDSQIGHLNPDDSVTVDKGLKPFPVKLTASGYPVDSPYSLLTYGVRAVTFLKFNVYGLGIYVSDQDLIKIPKVFNSAFLSKYMIDQDPNKTHKQNLTVALKDPKTSLNLISDLLDSEIAMIVKITPIRNTDFNHLKDGLSKSTMSHPDAKTHSEEMSKGIDEFKNAFTVKGSVPKNDDLFIKKNRDNTLELFHQKKTGEIKKLGVCNNPLVGKCLFAKYLSVPKPLSPSAQEMFVEKVVELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGQ2</accession>
    <name>A0A1E5RGQ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="10">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1142</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="10"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGQ2">
        <property type="interactions" value="707"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGQ2"/>
    <dbReference type="InParanoid" id="A0A1E5RGQ2"/>
    <dbReference type="OrthoDB" id="26387at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19672">
        <property type="entry name" value="UBR-box_UBR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.30:FF:000002">
        <property type="entry name" value="Putative e3 ubiquitin-protein ligase ubr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055194">
        <property type="entry name" value="UBR1-like_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF26">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22960">
        <property type="entry name" value="WHD_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="12">
        <location>
            <begin position="144"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="9">
        <location>
            <begin position="144"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1207"/>
            <end position="1227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1909"/>
            <end position="1963"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1918"/>
            <end position="1943"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="13">
        <location>
            <position position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ86090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1987" mass="229643" checksum="771FB9EAE1B5DF7D" modified="2017-01-18" version="1">MKVDPLLSDTIKDDLLKMHTLTMFERQDWTTRTVMLEHMEQYIFNYFYMTLSNDMELFSLMFPSLSLEEFPKKWRDLYPNLYPEGNTFHAIKICSKEFXSAKERAKTGEKYXKDVSVYTGPKSNHNIVEQSDPKESAVHCHTGRNCGRRFKAGEAIYRCQECGYDDTCVLCFHCFNPKDHENHHVYVDITSQGSHGTCDCGDTEAWLNELHCKAEHYALHGDSEFQEQRKKIKEYGPLFETILRAALDYFIDYFNQNFESMPKFTADITMKLRTYIQTGAVEKKNELLKKIAYKNKYISVDENFMREPKNYCVLVYNDEFHNYSQATSALSQGDPEDSRLEYLTNVIDGEGRAMLRSSTDLSKLLGGFFAVQSNGLSATLNSWAEYIHQETCNYLIMWIKDCLKIEHPEFQSVFRNALCNVFCEEVGFAGYEESNDVSETINELFPDMXSKQADELHQYADLSVLGEGNKLPQGHHLMMGSNYLNSISPHVNPTSEVIKNYKNSRVQFLLYFDNRYWKSLRRNVQDITIPVLSSSNVHRIDFCDQFAEIFTHMINSVCYLDREPQLTALRELILQSFSSHSHAIXMMETGSFLDVIWAIDRIFMNFSVLEDGALLWRRVNVINPSKGFAISFKQGVFSVETLLNKITSSDDLLDSQLFISFVTLFQLFNSAYKIKRKEGEHVLREDQFFISYMEYTMSIYNIIQVFEKILRSHFDEEKLFNAVRLLYTYLIHRVPSYKATVDEFEIIKFKVSKQRVAYMNPVHTFFSVLIEQLPLQKVIQLIEMYDKDFLIVSDYSLRSIVLCAQIDIGYWVRNGVTLLHQSTFYKKNPEICAYPRDIHINQIAFMATQNELPRSIYNMLERWEILDWISGDEGLNNSSYDDKFVPLIQQFIAFVYHLLSERSFYKXYASNEERNKAILKETIIYSLYPKPLYYSKLLEYVPDVLSEQLTEIDECLNDVADFVPPTGLADNGVFKLKENFYSCIDPLKLLNTKDDFEHSISIVEQNMEKINKVAGTEKNAKIIVSPVLEPYENMVEGSRKLGSFTRCDLFSKVLYKLFHYCLANEEYTFLYELLHLVHAIFLDDELANGATSFPDAYIKKPICNHLLSIVQNTKLSDNFKVKADYLLKFMLHKRPAEVQDSLVSCFGEQTFKSLYGQISSYTNNTDGETEMEKKKRQAKARQAKLMNKFKNRQSKFMKANKELYAGTNSQKTKSGLQDDETDTMPDASSVPVEDFSCALCQESETKGDLFVIPVYKSKNPGLRNPSSSGEEYLEEWNGFQNAEXNTNFYHHKKQTLHVSCSGSDNAFSSCNHAVHYKCLKKYWKKKHYKKSMFICPVCQTVCNKVLPYIAVENLSHETQKEKLNQIMNSEEQDNYNLLPTFSGTDNLKNNLVHISQFQSDYEDSYFNGKDTKQYIPYILAVQFANTIAMMEISSRLSKGTKHDFLVGKEQTYKTLKNMLLLISALKKVWKDCPNVHLPYSHQIYSSNPNKIFQFIAREMIFTKASLSTILHIVIKQMFYHFQHHGKKRFSSKFIKNLLSSGNECTPNSNVFNLLNIEDSXEMKTLIYTVVLRQLQCILRKVMIFYKVVEMTFTNRISSDSFFKKVELNAVNVPNDVESYCDFMIKMLTGHQNLSDLLKDKKSCVHTKTDQNIDLTRIAYSKCQSVRCSADDCGVAGYELGESLEGEYTGVVKLTNLSEHLNTYITDMHSLKFNTHTMDDEFSKALDDRMDYEICLTCGAKLRSSPDNQYITKHAMKNCFNHYAIFFIPNQNQLRLFLEQPQCCAAMAAPYLNTHGETGKLAMRKGDLTTLSLKRYQHINELWLNMGIPGLTSRLLGDSFRLLITASTAAFANEFGAFRFANILIDDIEQTPASTDLDIESDIEDAVENSTFASEQDDYEGRYVYCTEDESEHEYSEGYSDEDDDGADDDDRAEXDDGAEDDDGAXGWQGPWNPDGNVLYWGHDIPEEFDEYDSNDEVYPNTHQLNTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGV1</accession>
    <name>A0A1E5RGV1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Acyl carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g1224</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Carrier of the growing fatty acid chain in fatty acid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acyl carrier protein (ACP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGV1"/>
    <dbReference type="FunCoup" id="A0A1E5RGV1">
        <property type="interactions" value="744"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGV1"/>
    <dbReference type="InParanoid" id="A0A1E5RGV1"/>
    <dbReference type="OrthoDB" id="448946at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099128">
        <property type="term" value="C:mitochondrial [2Fe-2S] assembly complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000035">
        <property type="term" value="F:acyl binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000036">
        <property type="term" value="F:acyl carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.10:FF:000003">
        <property type="entry name" value="Acyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01217">
        <property type="entry name" value="Acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003231">
        <property type="entry name" value="ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00517">
        <property type="entry name" value="acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002148">
        <property type="entry name" value="PRK00982.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863">
        <property type="entry name" value="ACYL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863:SF28">
        <property type="entry name" value="ACYL CARRIER PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="13 14" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 14" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Carrier" evidence="15">
        <location>
            <begin position="43"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ86128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="123" mass="13665" checksum="22E50C2F4AF4A920" modified="2017-01-18" version="1">MFRSLLKPSFVNTMAKQAVMTQPRMTVQAPRMMMMAYSTLNKDDVAKRVIDVIMAFDKTKTQSTAVTPASAFHKDLGLDSLDTVELLVAIEEEFDIEIPDSVSDELKSVGETIDYISSNPEAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH62</accession>
    <name>A0A1E5RH62_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein TEM1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1201</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH62">
        <property type="interactions" value="575"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH62"/>
    <dbReference type="InParanoid" id="A0A1E5RH62"/>
    <dbReference type="OrthoDB" id="6585768at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017231">
        <property type="entry name" value="Small_GTPase_Tem1/Spg1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037527">
        <property type="entry name" value="Small_GTPase_Tem1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="3">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="293" mass="34314" checksum="C9744CCB79658E37" modified="2017-01-18" version="1">MTEYENGQQQQRERIRGQERESYDEQERESYDEQERERIREQERRINNEQQRRVNNEQQRRXNNQQRSTTNDNVSSNKNTHKDLDLQDNLIPRTTFKLKVGLVGDAQVGKTSLMVKYVQSVFDDEYIQTLGVHHLEKTEFLKYADILFVINDLGGQREFINMLPIVSEDAVAIVYMFDLTRPETLTSIKEWYRQAKGLNNKAISLLVGTKYDLFIEMDSEYQEKISKIATLYSEAMNAPLIFSSTSESINVKVIFKIIVAKSFNLKLTIKEIXEIGDPLLLYKNLGNKHRISH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH74</accession>
    <name>A0A1E5RH74_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation of fatty acids protein</fullName>
            <ecNumber evidence="10">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1320</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>an acyl-CoA + malonyl-CoA + H(+) = a 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:50252"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:50253"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ELO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="10"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RH74"/>
    <dbReference type="FunCoup" id="A0A1E5RH74">
        <property type="interactions" value="472"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH74"/>
    <dbReference type="InParanoid" id="A0A1E5RH74"/>
    <dbReference type="OrthoDB" id="434092at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030457">
        <property type="entry name" value="ELO_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF157">
        <property type="entry name" value="ELONGATION OF FATTY ACIDS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01188">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="229"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="305"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="310"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ86259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="329" mass="38121" checksum="079129902AB10870" modified="2017-01-18" version="1">MDSIQSIFSDSVKSGVIEYGYPSIQRPFGLHLWPLFSKAFECITGYPAEDFTFVHNKTFLANGYQAIGVIIVYYIVIFGGQFLLRTFNARPVKLSFLFQLHNLILTSVSLVLFLLLVEQLIPMVTEHGLFWSICSPQAFAPKLVTLYYLNYLTKYMELFDTVFLILKRKNLLFLHTYHHGATALLCYTQLMGQTSVEWVPIVLNLWVHIVMYWYYFLSSQGIRVWWKEWVTRFQIIQFLIDLVFVYFATYTFYSHKYFDGILPNKGTCYGTQDAAFYGYMILTSYLFLFISFYIQSYRKKPSAKKSVNGAAPAKASPAKAAKSTKSRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHA2</accession>
    <name>A0A1E5RHA2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA helicase</fullName>
            <ecNumber evidence="4">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">AWRI3579_g1069</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHA2">
        <property type="interactions" value="584"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHA2"/>
    <dbReference type="InParanoid" id="A0A1E5RHA2"/>
    <dbReference type="OrthoDB" id="3971953at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18041">
        <property type="entry name" value="DEXXQc_DNA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050534">
        <property type="entry name" value="Coronavir_polyprotein_1ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026851">
        <property type="entry name" value="Dna2/JHS1_DEXXQ-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014808">
        <property type="entry name" value="DNA_replication_fac_Dna2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788:SF8">
        <property type="entry name" value="DNA-BINDING PROTEIN SMUBP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08696">
        <property type="entry name" value="Dna2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="17" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="16" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12 25" id="KW-0347">Helicase</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication factor Dna2 N-terminal" evidence="22">
        <location>
            <begin position="485"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="23">
        <location>
            <begin position="1050"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="23">
        <location>
            <begin position="1208"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="24">
        <location>
            <begin position="1262"/>
            <end position="1528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="250"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1416"/>
            <end position="1452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="21">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="37"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="275"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="1421"/>
            <end position="1434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF08696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="OEJ86246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1601" mass="182991" checksum="66BF6138012D5EA0" modified="2017-01-18" version="1">MGSGFFDSSKERNDSKDFKKNRKISIMRKAHIYSFGPKNTLQQPKSTTHVSKIENKDSAPTQGTRTHEKLDSPVALQGSPTAELANDGEYPFQTKRKKPDAQVPKTLKRRQAXSKSTEPNSSEIFWNYSPTANPKSDGSFAEEGHLKVQSSPSTQDRISDSLKDSAKRNSNLLDINKRPSSGALLIDKKFGTVLNLKNIVSTTSSSPTTETIASARAKHFTNLPGNDSFDKDMDDIIDGLQTSINKDLLSSEPPQEEASPNKLSTPLQQRKKAHSLLQSSPLKSQSAANEELAFTQKYGQKVFDLLDQEDWSTSFSNTGSNKSGDRSVLLKSDPIIPSDKKTVKETVFDENKKRKKSLNTSIVLPKNFDTEDDESLLDDIEDFMTQKKTTNLGHLSHMHMPQNFTKHLERSRTFPLNKEDNVYTVKPEYIQLAKYSNIPGKNNKGNSYKWFKRLVIVNCENLELKNSADRKTLLTCCDEHGVIFDVELEGIWRHNSNYQSGQILHLVEFATSSHREMLAKKVFHLNTSSSILLILNPDTLLTATVVGTSMDCMRRTVINEKFSFPGDPSLVMLKGIIVHEFFQELLLLRNLYTHEMAEDILERLLLNNKKELVICGCDDFQEFKDNILETYVPYMEEFLIKYCKQDNNFGEVRVSGQMKKEKMEIKDVVEIEEKIWSPVLGIRGVIDAHLLLNEEQSVPFEIKSGKRSSNSHVAQSSLYTFLVQDRYDIPCEEFLLCYVEGKDAVKYYVHLGHLQHLIHYRNTLSVFLTDDEKSIEHGSSNPLPPVLEHGSEICERCYCNRECTILNYFEQQNESVKENFADRKVVQEILLPKHYVQDLVGTLPKNSKNFKFYKKYMNLIDLEESSIGYLNKEMFTMDSEERRLKTGKCLGQLELIEVFPIPQKNQYSLTFYFPQKNLLDSEFKIGKGDYVILNDEMGHFNLSSATVTSISHQKNRVNCVTSIDISKIQRRRAKKLGVSRITYRMDKNEVGLGMSLSRYNVLSLFLPENNFLRELIVEGRAPRYGNVSRTTQFSSLNSSLDTSMVXWEMFNEDQQLAIRNALECKDYSLILGMPGTGKTTVIIEILKCLIIGNGKKVLISSYTHSAVDNILIKLEEECKEMKDTFRILRIGNLSKLHNDTKKYSPYYKESDDVGESESLDQNEDMLDSLKQSNLVAVTCLGIQDWTFSSCFGGSEVEFQENDGSTGLRTKRFFDYCILDEASQVSLPIALGPLTLCDKFVLVGDHFQLPPLILNEKAKQRGLDVSLFKKLNDYYPDIVKKLTYQYRMNPEIMKLSNELIYDGQLKCGGKNVDNKLPKYGITQFFGKSDNLVNWILDCDKKVCFINYDQLSDFKHIQLFKEMVNNEDIKNQGEMEIIKQXVVPQLLKIITSDQIGIMSLYRGQLNLLKKELLEHGQYKENKEEEEEEEEEEEEEEGKSTLKXSTKNGKTFKRSKNNKKADYSKLEILTADQYQGRDKDCIMISFVRNNYDKNNIDQSNSKSKQYKILNDMERLNVAITRSKCKLIMIGSINTLKSVKNLKSFVSLLESQSWVYNLKDSDIPSSIELINESKKGGVSDKPGKPSLKRFTRNQPIIRDILAEHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHD1</accession>
    <name>A0A1E5RHD1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Stationary phase protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1461</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Stationary phase-essential protein not required for growth on nonfermentable carbon sources.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPG4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHD1">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHD1"/>
    <dbReference type="OrthoDB" id="4067991at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020485">
        <property type="entry name" value="Spg4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17325">
        <property type="entry name" value="SPG4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="86"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="108"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ86319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="145" mass="16416" checksum="DB5AC9C0885EE0EA" modified="2017-01-18" version="1">MGIFDAFSVHNPKKHADPNTGYQGSKNNTGSGTMFMYAKEYREPSNADKVIKAQGLSNDPEAFNKMMEEDDKLNREXGNMLVREDEYMGKMGGETGIPPQDRRGSDASMDSQGSTTVDWQHMSQNEFKKMYDSMRKGEPNNRVNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHF0</accession>
    <name>A0A1E5RHF0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Biotin--protein ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g998</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHF0"/>
    <dbReference type="FunCoup" id="A0A1E5RHF0">
        <property type="interactions" value="130"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHF0"/>
    <dbReference type="InParanoid" id="A0A1E5RHF0"/>
    <dbReference type="OrthoDB" id="10250105at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin--[biotin carboxyl-carrier protein] ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019197">
        <property type="entry name" value="Biotin-prot_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835">
        <property type="entry name" value="BIOTIN PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835:SF5">
        <property type="entry name" value="BIOTIN--PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09825">
        <property type="entry name" value="BPL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="1">
        <location>
            <begin position="382"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="697" mass="77133" checksum="1FEADBD2F1BFF8D9" modified="2017-01-18" version="1">MEQAKHRAHTINLLVYSGEEASSACVHHAVETLKCVLEPYYAVTTVSKKALLSEPWMTKTSALVFVGDSDQLYTQGCGSVVSKIQDFVRQEGGGFIGMCAGGTLGASRCEIYSDTSTMRVFRGKTLCFYPGVARDLAFPNAESKSHHSAKVVTLNTDNGTNVKSYYKSACSFISPESYGNVEVLARYSEVKGEMVIEAEDKNEESRDAAVVLCKDGAGTALLIGPQLEKVPEILLAQKERSAYETCVLKELTWDNENRKKFLRNIFARAGLKVNTYDCGAGAVPLTPIYLSVNESSSPKLKQLKARLNSYFVSPNHYNLKSSVDEFEIYEGFGNSEMRRYTSTLNRTYSEPKVVVLPNDNESCAPFANTPLFDAAKFFENKWSNTEYGSILLYGEVVTSTSAILDSNESFLSLFPQNSVVFMSTNQVKGKGRGGNVWVGPVGTLATTISTTFPLVSPVTGKPFSIAFIQYLSSLALCKAIKTYASGYENIPVKIKWPNDLYIMDPQYYFNNKLGIFDMKPVPLTDMEPSYVKCAGTMINSRPLDGAYSILVGTGLNATNLAPSVSIQLWVDIINDELRKKKNFNGKDLLAAVEHEKLLAMYVNHLNAIMDSFINEGPKGVLNEYYQHWLHSSQIITLTERNSVRAKIVGITEDYGYLIAKELMIGSDDFFTGTVHHLRPDGNTFDIFKGLISKKVYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHL7</accession>
    <name>A0A1E5RHL7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein PUN1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1241</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHL7"/>
    <dbReference type="FunCoup" id="A0A1E5RHL7">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHL7"/>
    <dbReference type="InParanoid" id="A0A1E5RHL7"/>
    <dbReference type="OrthoDB" id="4480814at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009571">
        <property type="entry name" value="SUR7/Rim9-like_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052413">
        <property type="entry name" value="SUR7_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28019">
        <property type="entry name" value="CELL MEMBRANE PROTEIN YLR413W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28019:SF2">
        <property type="entry name" value="CELL MEMBRANE PROTEIN YLR413W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06687">
        <property type="entry name" value="SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="303" mass="33969" checksum="F53463EAB91047E5" modified="2017-01-18" version="1">MYLSTLFLALFFNIAAFVLGIFALLGSTYNSEPLNKIYAAQLNLKNMSLEKVFPTVASQLSSSSSLTLSSFGLPSYINFGLWSYCTGNTTNTVYYCSKPNGIQNLNFNQILEDQITNTEVLSLIDSLADILLPSSFSNDINTFNKLAKAMFLCLIIAVVLLAIQMIITIVKIIFCWFPSTFKIFTLWILRVIAFLSFIGVAISAAISTAVYSVAKKQINNNSSDYGLELSMRPAFYGIIWGAVVASFINLLLVCLFCRLRDNKYYQSQQEIQPIFIPTGQQAGPYQTKQHDLPKFERTNSYYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHP1</accession>
    <name>A0A1E5RHP1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Post-GPI attachment to proteins factor 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1594</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the lipid remodeling steps of GPI-anchor maturation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PGAP3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHP1"/>
    <dbReference type="FunCoup" id="A0A1E5RHP1">
        <property type="interactions" value="179"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHP1"/>
    <dbReference type="InParanoid" id="A0A1E5RHP1"/>
    <dbReference type="OrthoDB" id="419770at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007217">
        <property type="entry name" value="Per1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13148">
        <property type="entry name" value="PER1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13148:SF0">
        <property type="entry name" value="POST-GPI ATTACHMENT TO PROTEINS FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04080">
        <property type="entry name" value="Per1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2 7" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5016479659" description="Post-GPI attachment to proteins factor 3" evidence="7">
        <location>
            <begin position="23"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="294"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="357" mass="41856" checksum="6F1A8171F6B83010" modified="2017-01-18" version="1">MKTASWKSLLLASALFAATAIASPGDWLDEFIDCKIVCESRNNCPTKEEPLEPYSNSFPQDHTAFAKVPFVYKTVFLWDCNDDCDYQCQQLVTRARIEKEEEIYQFHGKWPFIRMLGMQEFFSAIFSIGNLIPHYRGFRMLEKELWKTSIRNSRYKRILQNYQIVSIMGMCAWLSSTVFHTRDLVTTEKFDYFFAGATVCTAFYAIVMRLLISEYAKYGNQISKIGGVVVSAIFACHILRLYIDWSYTYNMRFNIFFGVLQYVILVSLAVKNYFQYGLSWKNFQTDKDFQKVVLVPVLLVLSTCAGMSFEIFDIFIYKLQIDSHALWHLATILPSFWLYSFFIEDYYKMVQHKSKIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHS6</accession>
    <name>A0A1E5RHS6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ceramide synthase subunit LIP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHS6"/>
    <dbReference type="FunCoup" id="A0A1E5RHS6">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHS6"/>
    <dbReference type="InParanoid" id="A0A1E5RHS6"/>
    <dbReference type="OrthoDB" id="3979149at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="153" mass="17341" checksum="EA961DB79113F9A4" modified="2017-01-18" version="1">MPQNKIFTLIEYIFISLALIAAVEYFKFGTRQNYEWFHCTPVYEPLDISTSSTTTGGSFPSTAKKLHAVGGPSCDKRGEFKTLVKRITRDYDINKQRISFCIVETPEVSYRHYPVEDGNKGAPGYVAYVAEDNDLPGLLEIEKLCKDTSIYHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHT8</accession>
    <name>A0A1E5RHT8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulatory protein RXT3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1253</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHT8">
        <property type="interactions" value="189"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHT8"/>
    <dbReference type="InParanoid" id="A0A1E5RHT8"/>
    <dbReference type="OrthoDB" id="3596986at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013951">
        <property type="entry name" value="Rxt3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08642">
        <property type="entry name" value="Rxt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="2">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="371" mass="42183" checksum="6E4ECB5E94BB6C91" modified="2017-01-18" version="1">MTESLVGVTSNQLNDGNXNAPTSKTAPQHSPESSYKQTQNEIYKFQETLLNSIKEKNNDSRSNTIDTNTSTRAAEEDSDFLQSKEKTNERSPVQSSVTDNNDTLSKPSDSLSFPYVSSKTAQSTINGTTLSSFKVFEYVKKHYDENIGKVIGVMKYHPYKEGTLIQKPTVQVSTNAGEVDVCHVLDNRIPXNTYDIGTKYNDYVLTSQVSPFIPNLDSLLQTREMENDFCSVIGKIXQVYIPHDAYFTEYVDYPRFANNEIWGYDIYTDDSDVLLILKQQELKKSQVFNGFNTLKYRETGESFFSNKHSTSNXQEHSFDILAQFVILPTLENYYGDLAQNDIFSRPWYGTQFNSSHDGYSIALYDAEPVYX</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHU4</accession>
    <name>A0A1E5RHU4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Maintenance of telomere capping protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1446</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHU4"/>
    <dbReference type="OrthoDB" id="3972155at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="343"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="343"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="399" mass="46177" checksum="3A29FC6A12175A8C" modified="2017-01-18" version="1">MSVVHSKYPDFIHTFIYSLVLGKHFIINTGNYNHRTGSSIEYVASALVDFLSSFKWIKYRILDCSSADDNYFAEKLESMSHRSSEIHNRHEVLIIIPSNDIKNNFKDGVKIAYFLQSVTRLTKNVGMKNGTTFSAVCVSNMDVLNQHQYLYKQFWLCCSLDSFVNQIQQNQAITVNNLNAYYLKFIKTWSHIQRSGXATIFHSSLRQYILDIVVHLRVHRFSVQGKAGGAQTNSLKDMTFLAKLFALTPFIYKQLYAESSDVELDNCLVTPDFIKQACFWYFPFKLQLSISKEHMKHPNFIKDPSILYGSDXALVDDLCNKLQDLQLMKRKQYQKHYNNTQGNWQQGAQAAQQDSHEQTPEQEYVGQRQDKDHVSQTEADFFFQLLVVKDVLNNVVPPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHW6</accession>
    <name>A0A1E5RHW6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Serine/threonine-protein kinase TDA1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1036</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHW6"/>
    <dbReference type="FunCoup" id="A0A1E5RHW6">
        <property type="interactions" value="256"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHW6"/>
    <dbReference type="InParanoid" id="A0A1E5RHW6"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="52"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="116"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="492"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="564"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="503"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="568"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="603"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="616"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="650"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="239"/>
            <end position="240"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="8">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ86477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="668" mass="75463" checksum="8905F492C3EC64DD" modified="2017-01-18" version="1">MTAKLHAPWPTFAGLQNTNLNNEHDDKNNTNDNHLTTKEELEKQICKYAIIQKDDEQLGDGQFSVVKACCNIKTKKKYAMKLIHKKLILDKLRLIQREVGILRLLRDEVKECEINASGKEATTDEKNNDTNNENQNQTENQSSTSICSTTSDSSVEEDCETEYLFKGHHHVLQLFDFFETKQHIVLITQLCDRGDLYDYIISNGSLNLNKTVRPYTACILSGLKFLHDNGIVHRDIKAENILFRMHSGNIVHNTTVKGKDNKKECPYDLKSHDLIIADFGLATFVENTSDMREYVGTLSYISPEIVACQHTKTMEYSDLIKIPSYDYKVDIWALGVLCYFMKFGYMPFDCETEQESLDCIKDGDYYLEEDEEEKLKKEHESHVEKEFLNFLQHCFTVDQHQRPSSTSLLSHPFVNKFFHDTVDLDETALQEIRPVLSTIQRSKSTASIYMKRPPSRTSSQNSLKSLSNHSASHVFLKNSSLTSLTSLAKDNLVAPNPSHHNHNYSSQPDNDNKNLHYSQLNSTFKLEPEISENYLMVGSYCESPSVFSSYTNSPMLSRGNSAANITMSSSSGNNNNNNTISSNSSNSKTAPSASSNLRMSAMSNNTGSTSNTSVSLMSFPNSQSNMGKYSSSNLKNNVPRSITPLKEEPNTAQNKETSNENAHPVFYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIB0</accession>
    <name>A0A1E5RIB0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Pheromone a factor receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1502</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RIB0"/>
    <dbReference type="FunCoup" id="A0A1E5RIB0">
        <property type="interactions" value="109"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RIB0"/>
    <dbReference type="InParanoid" id="A0A1E5RIB0"/>
    <dbReference type="OrthoDB" id="2874149at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004932">
        <property type="term" value="F:mating-type factor pheromone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14966">
        <property type="entry name" value="7tmD_STE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001499">
        <property type="entry name" value="GPCR_STE3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28097">
        <property type="entry name" value="PHEROMONE A FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28097:SF1">
        <property type="entry name" value="PHEROMONE A FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02076">
        <property type="entry name" value="STE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00899">
        <property type="entry name" value="GPCRSTE3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0589">Pheromone response</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="156"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="208"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="265"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ86627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="458" mass="52618" checksum="9195F07F1545BE75" modified="2017-01-18" version="1">MTFQPAIVALSSLALLALIPPLIWHTKTKNISALMTIVWLFLYNLKSLVDAAVWSGKGLAFFNSWDGKGWCDIMIKIEVGAYVGVPCCIARIAYELTEIIKAETILPDRTQVSYLCKEFAIVTFPPIIAMVLSYFVQLFRFGIFTYSGCQNIFSTSWVMIVVYAVWPFVVSVVALILSTRLLIVFYIKRKDARDILQCTKSGLTLTRFSRFIFFCFLIILIMFPSSLYTFIKTCKKVTGTYSYPKIHENDSWWTIFKLANEKPLLSIWLFHTMSYCAFFIFGTSSDALKMYKTALRAIRLGFILDYFKEKRLIKRKTVANNFLLKHVINSNSSTKPTLVNTPANTMTTTPGTMVSSAKYEFYSDDEYSYIQSHDYQTDYLTNPATHLNKSTKADLEVGFEEYEIEYKNHNRTAAKDSPLNERKILDIIKKTSYSNSSNKSVDDTSEEINFHFQIKNKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIP6</accession>
    <name>A0A1E5RIP6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Gag1-like clamp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1252</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RIP6"/>
    <dbReference type="InParanoid" id="A0A1E5RIP6"/>
    <dbReference type="OrthoDB" id="5576875at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053274">
        <property type="entry name" value="Fluconazole_resistance"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025124">
        <property type="entry name" value="Gag1-like_clamp"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28065:SF1">
        <property type="entry name" value="DUF4050 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28065">
        <property type="entry name" value="FREQUENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13259">
        <property type="entry name" value="clamp_Gag1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gag1-like clamp" evidence="2">
        <location>
            <begin position="407"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="461" mass="51340" checksum="DF1BD16F600AC65C" modified="2017-01-18" version="1">MGFTTSRSNTATSSNQSMPTLETSPSTAQQQNPANKETHSLASSIFKSISRVKSKITEICSRSLNAFSASGSSSEDDNFLADGIRQIYHINNSNNTSKRKLQKTSKTRNTVDDSYYGEPFPREHGRAGSGSSKLGYPSVPFFLSSSKTISLPSHLLCSTSXCYPSRNYSNNKSHKNSKNFKDFSWKECEKIVNGLFAEKDNSSLTSEQINILVNGKSGPITNVKNNKLQNRPSFLHSINISTKPDTTGLENNNTRVDINTYSKRSLSEGNKNNASVNDTQIDKNSMDSPTTISNSSFTTHTQKTQDTNKETLDNNNNNNNNTVASVAAGDIDSDNIESDDELEADECTDDEANFDTFDVYAEVAKLRNDPSSDFISGYPVWEKRRELQYKKYPINVNKDYDSLVNFRKVPEKAYYQVYEKLIVKNNPLKSPINLRDLIKVINVGWIVTRKYEEAAAGGSIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN09</accession>
    <name>A0A1E5RN09_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g627</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN09"/>
    <dbReference type="FunCoup" id="A0A1E5RN09">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN09"/>
    <dbReference type="InParanoid" id="A0A1E5RN09"/>
    <dbReference type="OrthoDB" id="3973137at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37451">
        <property type="entry name" value="MARVEL DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37451:SF1">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="7">
        <location>
            <begin position="75"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="291"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="291"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="340"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ88267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="385" mass="41817" checksum="4454A96B64A6F4B9" modified="2017-01-18" version="1">MPTTYNQQSNNPSGARPQTTYANTSATGATEPGGTRPTGGGTDDYYQDHNDEEVAAKSPQNFTGRLANFFSQDLPLMFLRLTELAASAIVLGLMAYSRRSYGVHNGKNNFVIAVSVISLFYLLMLYAILFALRMIMVPGIFFIYEIILMLLWLAAFIVVAWEHGRSSCSVTDFGNGTYYNNYLGSLTTQTYGRACRSSKTAIAFAGLAWLLFTISTFLFWKNVIRPIRKDQYKGFSDIWKSGSVMNINLHRMSGLKLADYTPPSAQTGHDLEHGGTGTYDNAYATQGGAGAGAGAGAGGAGGATGATDEERYNTYGSSNNNSTLQSGSNHYNEKHERKPSSGTQPYSAADTNAIPEEEQVGDASRNQQNPATTTTTTTQYQEQKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNG2</accession>
    <name>A0A1E5RNG2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">fructose-2,6-bisphosphate 2-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g994</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the phosphoglycerate mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.46" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNG2"/>
    <dbReference type="FunCoup" id="A0A1E5RNG2">
        <property type="interactions" value="457"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNG2"/>
    <dbReference type="InParanoid" id="A0A1E5RNG2"/>
    <dbReference type="OrthoDB" id="267323at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003873">
        <property type="term" value="F:6-phosphofructo-2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000005">
        <property type="entry name" value="GpmB, Fructose-2,6-bisphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003094">
        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013079">
        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF44">
        <property type="entry name" value="6-PHOSPHOFRUCTO 2-KINASE_FRUCTOSE 2,6-BISPHOSPHATASE LONG FORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01591">
        <property type="entry name" value="6PF2K"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00991">
        <property type="entry name" value="6PFRUCTKNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="6">
        <location>
            <begin position="19"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="6">
        <location>
            <begin position="133"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="530" mass="61024" checksum="08228710946E558A" modified="2017-01-18" version="1">MPTTNAFNNTSSADLINSSSNTNQDVQVCVIMVGLPARGKTFIANKIQTYLRWLMINCQVFNVGNYRRKLCEAQEENLNGEIAADGVQKDIQAEGLLDGENLDNNFESVLMDKVNTASSSTTSTTTTTTTTTNNNNNNNINASFFDFNNEEAMVLRNQAYVSAIDDITNWFQKSKKNNVAILDGTSCTRKHRKWILSKLRRLSIEPIFIETFCDDDKLLQSNLMDLRKNSPDANDNMNFKQKIRYYEAIYESLNKYDDEIVYTFAKLIKSSNNIEQVIINKIQSYLESRIIFFVMNLHYLNPHKVVWLSRHGESIYNIEKKIGGNSLLSERGELYSEKLPSIIKNECNVSDELIVWTSTLTRTQQTAQHLPYKTKLTWKALDELDAGLCDGMTYEEIESKYPDDFKQRDEDKYGYRYRGGESYRDVVIRLEPVIMELERQQNILIITHQAVLRCIYSYFMNIPQDQSPWVAIPLHTLIRLEVRPYGPCKVTLFKANIPAVSTYKERGTSKLGIDNSINNNDYRNLLINQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNP4</accession>
    <name>A0A1E5RNP4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">High-affinity potassium transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g969</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RNP4">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNP4"/>
    <dbReference type="InParanoid" id="A0A1E5RNP4"/>
    <dbReference type="OrthoDB" id="9999863at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140107">
        <property type="term" value="F:high-affinity potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004773">
        <property type="entry name" value="K/Na_transp_Trk1/HKT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051143">
        <property type="entry name" value="TrkH_K-transport"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00934">
        <property type="entry name" value="2a38euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31064:SF30">
        <property type="entry name" value="HIGH-AFFINITY POTASSIUM TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31064">
        <property type="entry name" value="POTASSIUM TRANSPORT PROTEIN DDB_G0292412-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0630">Potassium</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="111"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="791"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="862"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="924"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="187"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="654"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1175"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1405"/>
            <end position="1450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1465"/>
            <end position="1518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="241"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="260"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="278"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="300"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="317"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="388"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="399"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="452"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="534"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="684"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1182"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1483"/>
            <end position="1500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1509"/>
            <end position="1518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ88484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1518" mass="172557" checksum="5C27F0C63A1309BA" modified="2017-01-18" version="1">MSNMLRSLTRTSSITPFSRHQFSSKQSWGHKIRDTIDEVATYMRPILRLCFPSFIAFHYFYIITMTLIASLLMFPVHGQRYIDVLLIAASTTSQGGLNTINVSSMTLYQQIIIYLICCVTTPIFIHSSLAFVRLYWFERYFDGIRDWSKRNFKMRRTRTILQREMTGDHSRSGFGKSHARTNTGLVFSRTKTNNSHHLDHKNTRLKTNTRTGSRVKTLQNNAQPEKTDTRRSRARTHSKRLRTDETAEKSDGRTKGKLNPTKSQVSQQSNNDFQSRLFRGEMVRRYEADDDDEADGTVPSEKSGTSTESSDQNYAEKTSDPDTEHADPSSSPNAPDSEDKGGLQLHFEIPQKPKHTHPTTNTFHHTAPFSPEPKHKSSFNLRKPSFISRLSFSNTSAKSKNDSAHRSSNDSASDRKESSETVPKHPFSHLNKVSDLSYETNAIDFEDDDEDNKEKGPHQELLGDKHSMDTFPLKKVITTGDIPSTAQSFAGDANKMENDSDVQSNNSAYSDPLSGGSDAFETQGLANAGNGDHDDVEVEGEEEDENDGNNGDDHLRNFNDFVDDDVFPKRNSQRNMDQLVNDPVFQKQLYKKWKKERKSSKFLANYNRIHSRNPVPKARTQPTLPEYGNIDYEDMVDSDFANNYRNVKVSNEKAAKNDQQTHRNSHHKHADSDNNLSHSGDMENGNEDEDEDYDYDYDDNGNDDEDYDDEDFDDEEYDDDDDDDDDDDDDDDDDDDGDDDDDDDLVVDDGNNGNDIIRIGRNSTFVGLTEEQKEELGGVEYRALKLLCKVLTSYYLGFHTMAFLVFVPWINSKRTLGFYRDVVRDDGISPTWWGFFTGMSAFNDLGLTLTPDSMASFSRASYVLIFTSFFVVIGNTGFPVFLRFLIWIFQKFAPDLSQRQESLQFLLQHPRRTFTLLFPSGATWWLFLILIVLNVFDWVMFLILDYKADVVKTLGGAWYRVMNGLFQSFSTRTAGFVSVSLSGLAPAVQVSYMLMMYVSVLPLAISIRRTNVYEEQSLGIYNGSGKFKFMNTAKLGNHNANKNGDQNATSSNPVDADDLEGNTKSFIGMHLRRQLSFDLWFLFLGLFIICICEGDRIKDVDEPYFTVWSVLFEVVSAYGTVGLSLGYPGTDTSLCGEFNTLSKLIIIALLIRGKHRGLPNSLDRAIMLPSDKMKKLEDRNSSKRHRGKRRYSSVPTGETFQLQNIHTGADDNDIDNTMGTSPYFGGDGNNFANNDLEYSYSSPYDYNNNNNNNNNNNNNSNAGLQMARTVTRDPLLAYWKKRTHQAKNIVQETGNVLFHSSNAHGHEQLNENHRENNMYHPEKFHRSNTMTVGTLLNANDDNNDKNVNNMNNTRNINHNMDQEQNQNNLKRKSAINKLRRYHTNASSYASPAPNDFDTNVRFDEQNKVNGDKQRGKRQTFTVDETKNDSNNKPATSGYGDETSSNENDLYKSYGQTMDADELQGLIDDSEGDDGFVNETQYAQKHEALHLPKDDKQKDYTQENDAAQIHEQNQDAASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNW1</accession>
    <name>A0A1E5RNW1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Exosome complex component CSL4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g583</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNW1"/>
    <dbReference type="FunCoup" id="A0A1E5RNW1">
        <property type="interactions" value="339"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNW1"/>
    <dbReference type="InParanoid" id="A0A1E5RNW1"/>
    <dbReference type="OrthoDB" id="440760at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039771">
        <property type="entry name" value="Csl4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048626">
        <property type="entry name" value="CSL4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019495">
        <property type="entry name" value="EXOSC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12686">
        <property type="entry name" value="3'-5' EXORIBONUCLEASE CSL4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12686:SF8">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT CSL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21551">
        <property type="entry name" value="CSL4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10447">
        <property type="entry name" value="EXOSC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exosome complex component CSL4 N-terminal" evidence="6">
        <location>
            <begin position="13"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Exosome complex component CSL4 C-terminal" evidence="5">
        <location>
            <begin position="134"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="75"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="84"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF21551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="294" mass="31823" checksum="5A4C944FDE5ADFBA" modified="2017-01-18" version="1">MFDTSKYTGKVLPGDLICPVFDLDTEDTKVLYEFLSGHGSILDTYTAPNGQSYNVLVASLKGFIEFKELLAPEEDMEDGFEQDPENKNEKTHNASKKNSDRIVKKYTVNVVGHDPSLQSCVSTEQVNNDFSNNLPKENDIVLCKITKITQQRANAEILAVENRPCVADSGLGTNGSVLSSVTGGSGAVTFSISQVSSDVGETFKAVIRSQDVRATDRDSVVMTDCFCPGDIVRCKVLSLGDGTNYYLTTAQNDLGVVFAKSLGGAGELLYAVDWKTMVAPTSGVEETRKVAKPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP75</accession>
    <name>A0A1E5RP75_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Guanidinobutyrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g761</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arginase family. Agmatinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP75"/>
    <dbReference type="STRING" id="56408.A0A1E5RP75"/>
    <dbReference type="InParanoid" id="A0A1E5RP75"/>
    <dbReference type="OrthoDB" id="288726at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033389">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via agmatine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11592">
        <property type="entry name" value="Agmatinase_PAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.10:FF:000014">
        <property type="entry name" value="Arginase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="ARGINASE/AGMATINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358:SF26">
        <property type="entry name" value="GUANIDINO ACID HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00116">
        <property type="entry name" value="ARGINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184849" evidence="5">
        <location>
            <begin position="21"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ88503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="435" mass="48317" checksum="0013CDF34430C288" modified="2017-01-18" version="1">MVSIVVPLLAMTTVLQSVASNPIYNQHEQQVQAQFQSQKESLQDVWGQAWPFQGIESFAHLPHSQCLLNRSLDFDIGVIGVPFDTATTYRPGARFGPSGIRRSSQRQSSMRGFNFRAGINPYENWAKVVDCGDIPVTPMDNGLALKQMTAAYENLIINRPSTNSTNHTLTDEQSLAKPPRFVSLGGDHSILLPILRTLHKVYGEIQVIHFDSHLDTWSPSKFPSFWSSNTSDFNHGSMIWLAKQEGLIAPSGNLHVGLRTRIAGTDWDDYDEDDEVGFERIHADDILTRGGVQGIANHILEIVDADKPVYISVDIDVLDPSSAPGTGTMECGGFLSRELIYLLRKLEDLNIVGADVVEVSPPYDPLEITQLAGAQVAYELITNMVKKGPINDKLFNEERLASIETMNVKDIAELKHPNKGNVFKKYQRQYGRGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVD6</accession>
    <name>A0A1E5RVD6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Aspartate-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="4">1.2.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g315</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the NADPH-dependent formation of L-aspartate 4-semialdehyde (L-ASA) by the reductive dephosphorylation of 4-phospho-L-aspartate. Mediates the second step in the biosynthesis of amino acids that derive from aspartate (the aspartate family of amino acids), including methioinine and threonine, the latter of which is a precursor to isoleucine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-aspartate 4-semialdehyde + phosphate + NADP(+) = 4-phospho-L-aspartate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24284"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.2.1.11"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:24286"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the aspartate-semialdehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.11" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVD6">
        <property type="interactions" value="351"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVD6"/>
    <dbReference type="InParanoid" id="A0A1E5RVD6"/>
    <dbReference type="OrthoDB" id="1894490at2759"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00016"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00463"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00464"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004073">
        <property type="term" value="F:aspartate-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18130">
        <property type="entry name" value="ASADH_C_arch_fung_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02315">
        <property type="entry name" value="ScASADH_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000200">
        <property type="entry name" value="Aspartate-semialdehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000016">
        <property type="entry name" value="Probable aspartate-semialdehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051823">
        <property type="entry name" value="ASADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000319">
        <property type="entry name" value="Asp-semialdehyde_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005676">
        <property type="entry name" value="Asp_semi-ald_DH_pep-lack"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00978">
        <property type="entry name" value="asd_EA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006416">
        <property type="entry name" value="PRK08664.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46718">
        <property type="entry name" value="ASPARTATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46718:SF1">
        <property type="entry name" value="ASPARTATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000148">
        <property type="entry name" value="ASA_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01103">
        <property type="entry name" value="ASD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Semialdehyde dehydrogenase NAD-binding" evidence="14">
        <location>
            <begin position="5"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="13">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000148-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ90920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="364" mass="39755" checksum="235F910B79DDD7F7" modified="2017-01-18" version="1">MGKKIAGVLGATGSVGQRFILLLNNHPDFDLQVLGASSRSAGKPYVEACNWKQTELMPKSVESIKVVACSATEFSKCDIIFSGLDADYAGPIEKEFVENGLNIISNAKNYRREADVPLVVPIVNPEHLDMXEAKLSKAKSENKPKPGFIVCISNCSTAGLVAPLKPLVEKFGPIDLLTTTTLQALSGAGFSPGVPSMDILDNIIPYIGGEEDKMEWETKKILGNLSADKSTIELLSDEEMKVSAQCNRVAVSDGHTECISFRFKTNEKPNVEQVKQCLRDYQCDAYRLGCHSTPQQTIHVLEQNDRPQPRLDRNRDNGYGVSVGRIREDPVLDFKMVVLSHNTVIGAAGSGILIAEILKARNLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVJ2</accession>
    <name>A0A1E5RVJ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription initiation factor TFIID subunit 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g294</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Functions as a component of both the DNA-binding general transcription initiation factor complex TFIID and the transcription coactivator SAGA complex. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. SAGA acts as a general cofactor required for essentially all RNA polymerase II transcription. At the promoters, SAGA is required for transcription pre-initiation complex (PIC) recruitment. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (via core/TAF module) and promoter selectivity, interaction with transcription activators (via Tra1/SPT module), and chromatin modification through histone acetylation (via HAT module) and deubiquitination (via DUB module). SAGA preferentially acetylates histones H3 (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac) and H2B and deubiquitinates histone H2B. SAGA interacts with DNA via upstream activating sequences (UASs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the TAF10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVJ2"/>
    <dbReference type="FunCoup" id="A0A1E5RVJ2">
        <property type="interactions" value="524"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVJ2"/>
    <dbReference type="InParanoid" id="A0A1E5RVJ2"/>
    <dbReference type="OrthoDB" id="154356at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07982">
        <property type="entry name" value="HFD_TAF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003923">
        <property type="entry name" value="TAF10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21242">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21242:SF0">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03540">
        <property type="entry name" value="TAF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017246">
        <property type="entry name" value="TFIID_TAF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01443">
        <property type="entry name" value="TFIID30KDSUB"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="175"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="208"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR017246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ90962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="250" mass="27696" checksum="88EC25BFFD8AFE56" modified="2017-01-18" version="1">MLGDNPFEEVHGNSEFNEEIDDIVIGRNENDNNPLNNSATNVDGAEKGDEDEDEDMDIEGDEIDEDSGTHGQKKSRLETGADNLFELPEFTRKDKTLQEILEMMQDNPPIIPDAVIDYYLMKNGMNLKDIRIKRLLALATQKFVSDIATDAYEYSRIRSSTSVQNANNGQAKARQLLMGQQQQQMAQQQEQQGNGTNDNNGNIGPGNSGTASSGGSQQQNDREKVVLTVNDLSSAVAEYGLNVSKPDFYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVJ8</accession>
    <name>A0A1E5RVJ8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Vacuolar protein sorting/targeting protein VPS10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g486</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVJ8">
        <property type="interactions" value="227"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVJ8"/>
    <dbReference type="InParanoid" id="A0A1E5RVJ8"/>
    <dbReference type="OrthoDB" id="443634at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.270:FF:000005">
        <property type="entry name" value="Sortilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031777">
        <property type="entry name" value="Sortilin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031778">
        <property type="entry name" value="Sortilin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006581">
        <property type="entry name" value="VPS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050310">
        <property type="entry name" value="VPS10-sortilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106">
        <property type="entry name" value="SORTILIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106:SF27">
        <property type="entry name" value="SORTILIN-RELATED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15902">
        <property type="entry name" value="Sortilin-Vps10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15901">
        <property type="entry name" value="Sortilin_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00602">
        <property type="entry name" value="VPS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110296">
        <property type="entry name" value="Oligoxyloglucan reducing end-specific cellobiohydrolase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184899" evidence="9">
        <location>
            <begin position="25"/>
            <end position="1648"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1472"/>
            <end position="1490"/>
        </location>
    </feature>
    <feature type="domain" description="VPS10" evidence="10">
        <location>
            <begin position="824"/>
            <end position="1458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1611"/>
            <end position="1648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1631"/>
            <end position="1648"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="11">
        <location>
            <position position="450"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="11">
        <location>
            <position position="453"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="11">
        <location>
            <position position="771"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ90967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1648" mass="185891" checksum="7BA0DE9565F3FC14" modified="2017-01-18" version="1">MIIRGVYAWSICVYILSRITGISADTSEPFKPKLYPIDAKPDKAPPSNSFYVFQDLIRFDDTPNLLFVDADAKVKFSENYGADWKLATGLDSLYDERKDKENNYESFPYLSPDRIYKHSRAYIYPNSEKGYFLYFTDDAGKTWEKWDFSTLIDSQISSYLPSKVQNFEKKSIYFYHVRSSAGNEKHLMVTVSLSDYKHDYYASFECLSIDLGKSWKVLTPIPKSAIQGNAFENACEFGKSSSESNANDDDILCVVSNRQELAKEEEKKVEDEATDKVTGQMFKSSNIGKTWSMIDSLKDVNVLYFEVTDIYVFAYASKDAFNPWSSVDIYVSTDNAQTFKKAVIAGEIRGEFKKGLMSSNYVQVVDDIVILHVQLKPDSSDSEDDFFQMPKEKTFISDSTGLTFYSLDHVAHGKGEAIDXAFSSVESVHGLKGTFYLKTNKIEVTRFEEDGQDXIXFNSTXLSDKISFDDGYSWSNVKINAVRDELLSNWDAQCDPSTDRSCTFKSYGTPYYIRGRETALTTPGIFAVNGIVTKGNTYLHEDKKEMQTFITRDGGLSFDFCFNFSANIVYGDYGNILVAIPFNAESDGDPNAEIYYSLDQGYSWQEYQLDYAFAPMNVQSVVMDGSGFHFLVEGLQYDNIDDKYXXXKSKSVRFILDFSDVFEGSXCDQDKDLEVWELNNGECIDGVKHSFKRRKQFSKCSIKTLFEDLKDESSVCECTEKDFECAPGFLRDFSSGACELNYQLLAKQTDISCKSPKDKLKLPTRRLKSNDVCKNPSNVNIDMEEYTCGALGISDGDSKIIVYENDRLDGKPLTYQYFGSLKSDSLLIQTSAHSVFYSTDGGVSLKKFDSXGEDIVEVIFNKYNRSTAYMLGAENSLFVTTDSAFEFNQYKSIPTDSIQLRFPLDFHAKDSDQFIYYGGKDCESLRDPNCHAVAYYTKDGGATFEKLLENALHCEYSGSLFSKSVDENLIICEVRKPGDITSSIISSKDYFKNDYKVLFDSAVGFASYSGYTLFAVTTKDDELVAYVTTDGEEIAETKMPKVFESLKQKAFTALGSSNGALFFHLTTNQEKGKEFGALMKSNSNGTSFVNLERAVNRNSAGFIDFEYVSGLEGIIFTNIVSNSERLVDGKDSKKKLRSQITFNDGAEFRYLNAPQKDSQNRKYSCNVNDVAKCSLNLHGFTERTDIRDTFSSGSAVGMMIGVGNVGDELKPFNECGTFMTVDAGVTWKELLSEPYQWEYGDRGSIIVLVKAGKETDSIVYSVDSGKTWKNFKFSSTPVTVLDIVTHPVDASMKFFLLVQNTNSNVPTTVALDFSEVFERQCHFDFADSKTDDYDYFPLRHPESKCLFGHEVEYLKKIRDDCYIGMAPLKQANRITSNCSCTRDDFECDYNFYRSDDGTCKLVEGLSPLDPLEVCAKDEKLVEFFMPTGYRKIPLSTCQGGKQLDKSSSAPKACPGKEKEYKALHSMGFMSKFFIFVIPFCVMLFATWFVYDRGIRRNGGFSRFGEIRLNDEDDLVEDTLSDAIVNRIVMLGVASFHGLSSLWFYAKKPLVSLKNKVTGFTGISRISQNGPRYSSLIQDQFMNDADDLLSGHDEDADDLATLNSSDINFDINDVANTSEEDTNFAPYSDGEHEGDQEEEEEDDEDERNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVK6</accession>
    <name>A0A1E5RVK6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Presequence protease, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Pitrilysin metalloproteinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g561</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Degrades mitochondrial transit peptides after their cleavage in the intermembrane space or in the matrix, and presequence peptides; clearance of these peptides is required to keep the presequence processing machinery running. Preferentially cleaves the N-terminal side of paired basic amino acid residues. Also degrades other unstructured peptides. May function as an ATP-dependent peptidase as opposed to a metalloendopeptidase.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer and homodimer; homodimerization is induced by binding of the substrate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M16 family. PreP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVK6">
        <property type="interactions" value="680"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVK6"/>
    <dbReference type="InParanoid" id="A0A1E5RVK6"/>
    <dbReference type="OrthoDB" id="10250783at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000013">
        <property type="entry name" value="Mitochondrial presequence protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000011">
        <property type="entry name" value="Presequence protease, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013578">
        <property type="entry name" value="Peptidase_M16C_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055130">
        <property type="entry name" value="PreP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43016">
        <property type="entry name" value="PRESEQUENCE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43016:SF13">
        <property type="entry name" value="PRESEQUENCE PROTEASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08367">
        <property type="entry name" value="M16C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22516">
        <property type="entry name" value="PreP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01264">
        <property type="entry name" value="M16C_associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M16C associated" evidence="8">
        <location>
            <begin position="495"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="9">
        <location>
            <position position="927"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ90961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1030" mass="116939" checksum="B76895309C2DFB5C" modified="2017-01-18" version="1">MLRFKRLASSAAGTYNQARILRKYPVGATYHGYEVKRLFPVPELQMVGVELVHAQTGSQHLHLDRNDSNNVFSLIFKTNPPDKTGVPHILEHTTLCGSAKYPVRDPFFKMLNRSLSNFMNAMTAHDYTYYPFATTNLKDYHNLMDIYIDSTLRPLLNYKDFCQEGWRLENEKVDDKNSPLVFKGVVYNEMKGQMSNLQYYFYSKFKEAIYPSLNNSGGDPLHITSLYHSDLLSFHSKNYHPSNMKTFSYGNNPLQETLLKLNDSLKQYGKRYTRKDAIKVPLVLPATESGKAALKLTKSSPLDSMLPPDRQIKSSLTWILNNPNEELYETFLLKVLSVLLFDGTNSPFYKKLIESGAAYEFSCNTGLXSMTSKNFISVGLQGISSDFLTNEFEQTILDILKNDCMNDMSLYSKTKVQALINQMELSLKNQQSQFGLTILSHILPQWVNHADPFELINFTENVDLFKEDFAGKGAKLFTDLIEKHLINKPYFHFTMTGDEKFHEVLKSKEAAVLEEKLETLDPXDRKIIYERNLENSKESVEAKEDLSVLPSLQVSDIAKNGLDFPVNVDSKVFSRITNTNGISYVKFKKSMQSAIPLELLPYLSFFSECLTFLGTKTQDYDEIEDEIKLHTGGVSIGCRAQTSSHDINASELFLEFSGWSLNSKMEHIFDIWVKLLKDTDFSMVKXAKLKTLIKSLSSSTSSSFSDSGHSYAKSYAAASLSKTYSLSEHLGGVKESQFINKLNTFLNDESKIQTEIIDKLIFLQKHILHSLKNAPESGISKSFIITDEPKQVGLFAHGVDENFLSKMDIAQNEVSHDQASSTAKDIKLLPNQNKSVIDFPFQVYHTSKVLKGVSYTHEDGPKLQLLSTLLTNKILHNAIREKNGAYGGGASFSAVDGIFNFYSYRDPNPLKSMQIFDDLCDSEKSKDFNFNWTQQDLDEAKLSIFQRLDAPVSPRNEGLAFFEYGLDYEAKEKRRSQLLNCDLKSVFEANEKYLQSSSHNKDYAVAVVGDAHKDEHNFQEQNYNIIKPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVM6</accession>
    <name>A0A1E5RVM6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nuclear mRNA export protein SAC3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g311</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVM6"/>
    <dbReference type="InParanoid" id="A0A1E5RVM6"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024293">
        <property type="entry name" value="SAC3_helical"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12209">
        <property type="entry name" value="SAC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC3 helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="740"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="761" mass="87492" checksum="04C9451287BDC244" modified="2017-01-18" version="1">MSNNVGEFGLRRVTDQQXSIGLSNETETXEQVKKIIPSRQIKSTSVQQTAKTVKFSQNDLIQARRAQVIETMNEEQITNFCHQEIGEIIKENMAKYQIRESKLNSIAEELYTAFLREQVYMTYLEARAIVQCRKVSQRRWLKRWQVKLQHEXEHKAKTKRLXTDILKVEKSIGVPSKRCVSLDYFLKNQQGSPTIGGSFNSLXNNGRGXQLLTPIKNEKREISLLGCSCKIDLYQDYLEPILXTKQSLIKYCNLKNVGIDISIFSDNWKDTSSKWLYENMHIGMKVNAGDDLSLSVKGYDFDHXFDSNEQCNTQLLIFNTGVTGEDIFDLENKLKKDGEMLISLLNSKNFMKNHYRFNILLLYWESVDTKYDVNELYEFFQLSKIFEIFGHTLVKRIDFLKIKNKKSMENLKIGLKEIALDFKFDLTNDSGIPYFDYLKXQQRQEDNFSNKRLVSSLLRGNNDFDDASQXFMIKKNKIDDKLQKMLKEEQMKYMQQKTANQKNTFQFMKRYPKKNVLQDKSNRKVSVLNSDXELNETPRGITNSMHQMNNIAEEESFDDGNDESLIDSSYHSVIGPSXSSGQYIRPAIDSPVGIPVVTQLDERSKKLIRNLTFNYNGSKEDGLYEINGHQSKESNVKNGNSLPFIKTPTFESGSTTPSELMKRPLSHFFTPTILRKNATKQGERSFSSSMRTPSSRVVSINRLINGDNNSSKSNEXLFSNAPTVRKHKPYGKRISSVSSDASSVSELKNLVDSVVKKLENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVT9</accession>
    <name>A0A1E5RVT9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative glutamine amidotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g331</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVT9">
        <property type="interactions" value="107"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVT9"/>
    <dbReference type="InParanoid" id="A0A1E5RVT9"/>
    <dbReference type="OrthoDB" id="92161at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01741">
        <property type="entry name" value="GATase1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044992">
        <property type="entry name" value="ChyE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695:SF5">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="1">
        <location>
            <begin position="70"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="259" mass="29549" checksum="B6D84D3D23CA1852" modified="2017-01-18" version="1">MTVEQEKQEHSVIVLNTDCPKEYLQQLYGDFADQGIALMKRCDHSNYITSFSVFNCFENKFPTIPQLKEKSVVGIYITGSVFDSYSDEHEWIINLRKLMKNILTNHDEYPPIFAVCFGHQLVGKVLGSEVIKNPKGLEEGLLEVNLNEKLQDSLFDGKKSLYMSETHNDIVLSLPEGYENIGSTXLCTFQGFYQKGKLMSWQGXPEFNKDILLACMKNSVENGHIRQETYDMEAKRSAEIPNEGEYIGSLILKLFKGQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVV6</accession>
    <name>A0A1E5RVV6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g535</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RVV6"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="unsure residue" description="D or N" evidence="1">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="1">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ90878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="434" mass="47836" checksum="BD0F4A91018FF984" modified="2017-01-18" version="1">MTLAFGEGELTLSTDTPMQNTFEQGPCAAIVRVAKNANTTSSKMKGDARAKTAKISDHNMHNEGEKLVANSTPFVCTRIPALDAGLTVKXSALASGLPSSAHAQALSWWPESYSKSDECETMEETLAACASRTETLXAXSVVYIGRDXSFVGXATTGTRASNFXICDSKLLPYRANYAXCTKTSLFFSFFFHGSGTDRTHNHLDITNCGSGSDKHNGKKCAVDHGIDEAEVEAHENTQTLVTVKGRKNGHQQIVDHAPQSEASDXDTLLEMDRGAGKYKDGDTCSNNHNDHELKXDNTDVIESGXDTAGDRSKQIPEFSNTLTQKVLDEDLTIDELMNEVAAILRRDCGSTHDLKTAFCGSEDEDDEXNDIHEAIIARRYSXLKKREGFWAITRGEQEKDRIVTMTTWRKQNRRKVSMLNLCFEHVKTRTARTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RW69</accession>
    <name>A0A1E5RW69_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g355</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RW69"/>
    <dbReference type="FunCoup" id="A0A1E5RW69">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RW69"/>
    <dbReference type="InParanoid" id="A0A1E5RW69"/>
    <dbReference type="OrthoDB" id="2414723at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18316">
        <property type="entry name" value="BTB_POZ_KCTD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31758">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING PROTEIN YLR108C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31758:SF2">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN YLR108C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="20"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="420"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="336"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="420"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ91008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="617" mass="70177" checksum="2FFA929747CAFA95" modified="2017-01-18" version="1">MPDKSLAFNPEIPHVLPHEKMYKLQIGNKLFLLSGASLSSDAPSYFTKYFQKTTPNISADPPTTRVNDPPILFIDRDPEIFQKIYNHLQGYFIDIKDEVEYTKLFADAMYYNLPRLKNLLKNSNNYYTNIGGKHYVLNKQLFTKNKGNSPNYFTITSEIVYMDLENFFMQRNASKNAQKSPWENEIVLENAPLLRPPPQSPPMVNRSPELFDKLLIILNKGEIDFQMSYTERCNLIKECRFYRFQHLEQMLIKHAKINNPFIHKLEIVMNLLDVKLSGISSLNFSASSPDTQVDTLVGNNKQETTQLPKSYSEISSFSSDLTTNNHYPETNDRNSDSNSSSSICSSTPSTPHATTDETMHADKKRKLCAPGPTAHLKKCLQEKWASPSYKRPFVDEQYRKLIFQVGNSIALNGYDSCSYPQPSKSSSATSSRGTPVPMEAESQCTDCYNPYGSLFFDKKNKRMYIEFPMAQSSTTDTLAKIVDKVTNGKLGIKQKYAYMDMETQQEVLLLPCCTVVANLQINGKKISNLKHIILDPKCAGVFTEGNMIKSSDLALAENNYLNGSEALENYKMKGYRLHLCDASIWRLGFKTQDDLMMIAAKLNCCTNLKEYNDLTFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RYV8</accession>
    <name>A0A1E5RYV8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">inorganic diphosphatase</fullName>
            <ecNumber evidence="3">3.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pyrophosphate phospho-hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g269</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000002">
        <property type="protein sequence ID" value="OEJ92147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RYV8"/>
    <dbReference type="FunCoup" id="A0A1E5RYV8">
        <property type="interactions" value="261"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RYV8"/>
    <dbReference type="InParanoid" id="A0A1E5RYV8"/>
    <dbReference type="OrthoDB" id="1608002at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00412">
        <property type="entry name" value="pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="INORGANIC PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00387">
        <property type="entry name" value="PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ92147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="294" mass="33102" checksum="AB63D5FE8846B2BE" modified="2017-01-18" version="1">MFVSRRLFSSITSGTKYTPQFKQYLKLSDGKLGSFFHDVHLNPLNTSSKSSQDRSKGLSGNILNMVVEVPRWSNAKFEISKDLPLNPIVQDTKNTKLRFVNNIFPYHGYVTNYGAIPQTWENPSVNNGDNDPLDCCEIGSGILETGTVTPVKVLGSLALLDDGEMDWKVIAINVNDPLCAKINTMKDVETFMPGLLAGIRTWFKTYKIPTNKPANEFAFDGEYLSLEKTMSVVQECHEEWEGLMKLSSNVGQTTGHSVTTERFATYDLHESSLPNLNAREISQTVEFDQKWYYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZL0</accession>
    <name>A0A1E5RZL0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g261</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 14">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="14">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000002">
        <property type="protein sequence ID" value="OEJ92153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZL0"/>
    <dbReference type="FunCoup" id="A0A1E5RZL0">
        <property type="interactions" value="1382"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZL0"/>
    <dbReference type="InParanoid" id="A0A1E5RZL0"/>
    <dbReference type="OrthoDB" id="1662883at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045298">
        <property type="term" value="C:tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045143">
        <property type="term" value="P:homologous chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030473">
        <property type="term" value="P:nuclear migration along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000005">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000008">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 14" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="15">
        <location>
            <begin position="50"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="16">
        <location>
            <begin position="250"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ92153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="454" mass="50662" checksum="C4EA828D61A856AC" modified="2017-01-18" version="1">MREVVSINVGQAGCQIGNACWELYSLEHGIGPDGYLQEGLKKPKGGEEGFSTFFHETGSGKYVPRALYVDLEPQVINEVRTGKYKDLFHPEQLINGNEDAANNYARGHYTVGREILDDVMDRVRKITDQCDGLQGFLFTHSLGGGTGSGLGALLLEHLSNEYGSKKAKLEFAVYPAPQVSTSVVEPYNTVLSTHSTLTHSDCTFMVDNEAIYDMCKRNLNIPRPNFNNLNNLIAQVVSSVTASLRFDGSLNVDLNEFQTNLVPYPRIHFPLASYAPILSKERAFHESNSVQEITNACFEPGNQMVKCDPRNGKYMATCLLYRGDVVTRDVQNAVSLVKNKPTVQLVDWCPTGFKIGICYQPPTAPPESQLAPVKRAVCMLSNTTAIAEAWKRIDKKFDLMYNKRAFVHWYVGEGMEEGEFGEAREDLAALERDYYEVGKDVYGDEDEEPELADF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZR5</accession>
    <name>A0A1E5RZR5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphodiesterase</fullName>
            <ecNumber evidence="3">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g223</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="3">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZR5">
        <property type="interactions" value="129"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZR5"/>
    <dbReference type="InParanoid" id="A0A1E5RZR5"/>
    <dbReference type="OrthoDB" id="546632at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="5">
        <location>
            <begin position="284"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="104"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="672"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="672"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="686"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="702"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="6">
        <location>
            <position position="7"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ92374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="775" mass="87176" checksum="E1104FDED16D809F" modified="2017-01-18" version="1">MQEDSNDXDGSNSSVNTGKNASNKKLSVTNIYLVKADSGSSGGDTNSLTQTIEAIEKSHPNIRVTIFESITQLIVHIFELRLQGTLHFENYSTLIILLTGDDTTTSSQVRDTHSNGSYEKATHSNGSKKKDDDKNHVKNVPNTQHKLDILSPHELQNVFNKIFGLLQFHITGVPLAKTTTTLKQTILNYNGLNLKNFHPVLKNRLRRVTHWCFDTTDSQTASTTTCSDSTYSMYTQLSKLISTEPHDNNRVQEXDLNISELLAHIDLSQILRLPQGKSAKSYNSCLSTWAFCATELTPSELIQIGVNILYPLITNDYKDSEYSLQNLQTTNSNDKKQNLKTSTSVSKNEVFLLMFIVECNYHQDNKFHNFKHAVDVLQASHYLFLQLVGESCKELGLDSTQKTSXNSISNHICACSIKELTSMLLLLSLSALGHDVGHPGTNNKIMQENTQLIENFYGNSTDSILEKFHSKIFWNFLKDFPQVLDNCYSGKHHIDDIILATDMAKHYQYIEDLSTTSECCSENIKTNNTCLGNFVNCHKRSISSLDLDSTELTKXTQDLTKXNSCSVSDTCHMSKNSLLVLELIVKAADISNVTRPLLISTKWAYLICLEFAECIKLTEQNDNYTEAVAKAKAAEANAAEAEAFAEEMSGNGNEXKRMKEVAEKAALKARKEANFQTDKKNEHPQSKNINAHRTSVTFTSSSEDEDLDPEPELMDQCLSLQPSIPQGQLFFIKTFAAELFSKLSAKFPQHFAFLEKNLNENRKFWEEYIKEHIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZX3</accession>
    <name>A0A1E5RZX3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MICOS complex subunit MIC19</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g89</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZX3"/>
    <dbReference type="FunCoup" id="A0A1E5RZX3">
        <property type="interactions" value="60"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZX3"/>
    <dbReference type="InParanoid" id="A0A1E5RZX3"/>
    <dbReference type="OrthoDB" id="5544375at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012471">
        <property type="entry name" value="DUF1690"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07956">
        <property type="entry name" value="DUF1690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="159" mass="17806" checksum="D631868E9C494862" modified="2017-01-18" version="1">MGATQSKPQLVFKTESPVEFSDELLNQIQNISDNDYVRQQKAEQYIQEEVSKALATLEQETLSTFENKLNTSLLSKASESEDGVAPELSSKQLTDSVSSMNNHLNKLIDQQVNKVGSSVQKEEMQSIKSSLLKCLTDNKGKPLNCYEEVQKFKELAESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S017</accession>
    <name>A0A1E5S017_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleolar pre-ribosomal-associated protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5S017">
        <property type="interactions" value="173"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S017"/>
    <dbReference type="InParanoid" id="A0A1E5S017"/>
    <dbReference type="OrthoDB" id="160374at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052609">
        <property type="entry name" value="Ribosome_Biogenesis_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018849">
        <property type="entry name" value="Urb2/Npa2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15682">
        <property type="entry name" value="UNHEALTHY RIBOSOME BIOGENESIS PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15682:SF2">
        <property type="entry name" value="UNHEALTHY RIBOSOME BIOGENESIS PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10441">
        <property type="entry name" value="Urb2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleolar 27S pre-rRNA processing Urb2/Npa2 C-terminal" evidence="1">
        <location>
            <begin position="930"/>
            <end position="1137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1138" mass="129504" checksum="382883E22BCE5662" modified="2017-01-18" version="1">MASLPTNSESLSKYLRSKQIXVPELISIVDENYNGIYFPRGDLFVFELLVDRLNEQNNNAFLGNAEIWGLFNTLWDKVFRNYETTFLIRVYKKLRVYQILTNLQSLICANPALESQVGNFLDKVKKSSLSIDLPQPDIAFKLICGFSQQHPVSTDIIDKLLLLVDFDSKADQSRFLQYLCFKAPRSLLDLLALDDKKITSILYTFVFDNASLDGTQTLIKLLEKTIKTATPNDNATLVKLFELVVKSNSSLTESVFQQVSKISQNVVPALLESLSRLTKKNLISEEILNEVLTATLSSKNIQWALVADVLKLNIELGIKNSTLILENIEKSSTPLLSVWEQFVQCYVNAREFYVFVTDVLQTFSEKHPSSKLIFDSQYKSVISKYVANLSSTQLLTVINKLLDFPHLLLLIVKGSFNPVSLQYVKDSAEMKTVLHNIIFSDSFTQSVESVEGVNWELAFNVMELYYSCFNSQQDTDDIKEHYESLVKIQQQKSAEQQSKYSFFSLFKLRELVDYSDKLIVHGFMEWFATVSTDNEXILFDLFTRWSSLVNSVFDKASLNXLCDELVTRYPIVLHRILTTSVVSNNDFFEEEEIMYELATIISKSVIDKGEFSNVDFICLIPVQCWNKNIRIALINFLVENLQHLSNNHAIIYLLKESPTFKSIVETDFDKLTRTLEXGSEIFDCVWANHLIRWKESISYLNESLSVLIKKTVXDDCALRASLIVILSGHSVDNCVFTESWEKLLHIFVAHCYETIQSKEASMEYLYQVLSSCNFSXKKEFIHDXIKPLFDSHTVGNFSVGMGNLXLANEKKVCNTFDLYLTTLNTKDEILXGIAHYLVLKSXGLDPQGLSTFVDRXTKTDAKLFQAIFEETVQCIGSCVSYEAPILGVYQTLLEHVPSKNEKLVTPFAHSVGQLIVWSDNQASVAPLLPVLETIKNLLITQQWIFSQYTIESLFPLCCKTVSQCSLNNEAELSNTIEVTTQLLSIVLNFHSFRLTNRYHLINSIVCFYMDVLSHSPNVSVNAVQNVSRLITNYCEPSLSSTLSKNADLGLHSKVSLLKKNLRRSIGPVLLKYIKLCVKNYNPLSNSVRKELIGSIYSVFALLSQDELVTINASLDHAGKTYFKKIYEDFKTNGKWRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S021</accession>
    <name>A0A1E5S021_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carboxypeptidase Y-deficient protein 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g25</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5S021"/>
    <dbReference type="FunCoup" id="A0A1E5S021">
        <property type="interactions" value="833"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S021"/>
    <dbReference type="InParanoid" id="A0A1E5S021"/>
    <dbReference type="OrthoDB" id="3821113at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028934">
        <property type="entry name" value="Vps26-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12233">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING 26 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03643">
        <property type="entry name" value="Vps26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="305" mass="35169" checksum="7F0E63AF4A59AC1E" modified="2017-01-18" version="1">MSSFFKPPVDIDISLDDEDNRRVMELPNKKQGIMDRFPIYEDGESINGVVTLRVRSEGRRVDHTGVKISLMGCVDMRKTDSKSSSSNGTETPLMDTFLCLTADLYPAGELVHSQSVKFSFKDVQKRYNSYKGANVEVMYYLKVTVARKSSDVTKIKKLWVNLYDQPDSLVSQPVKLDIGIENCLHIEFEYAKQHYTLNDMVMGRIYFLLTRLKIKHMELSLITRERMTNSEKLSDSNSIRYEIMDGSPVKGETIPIRLFLSGYDLVPNMQLSSFSVKHYLSLVIIDEDGRRYFKQAEIFLHRVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S0A0</accession>
    <name>A0A1E5S0A0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Oligopeptide transporter 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g143</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5S0A0">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5S0A0"/>
    <dbReference type="OrthoDB" id="3970107at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004648">
        <property type="entry name" value="Oligpept_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22601">
        <property type="entry name" value="ISP4 LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="336"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="420"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="502"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="539"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="616"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="679"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="755"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="11">
        <location>
            <position position="785"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ92393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="822" mass="94208" checksum="22EE5181337246C5" modified="2017-01-18" version="1">MSDLEKKAATTVDVASKSTDDNGSSSKDHYITEEKPIEFITSIKSYSDEQVWHLLKVLNYDDVKDLNDLPPEVEFLGTKVHEITIDESLEIMKDAIEYHDNDPNIDEDQYQAFIKYAEEGVDPNNEVAVFELKALAVLLRDHSPYPEVRAVCPPAMMDDPTIPIETFRAYFFALLWLIFSAGFNELFSHRLVSISISTSVIQMFLYPMGKFWERWIPSWGFSVRGHRFALNIDTPWTDKEQMFCTLIIAISMGTFYTSYNILTQKIYYGQDVSFGYQFWLSLSVQFMGFGFAGILRRFVVYPVKAVWPTSLSTIALNKALLTPEDPNEKGISRYTFFFSCMIFMFIYTWFPTFIFNALNTFNWMSWIAPNNWNLAAITGGVSGVGINPIASFDWNVITSSSLLTPWFSMSTQYAAYSPPFYSAGNLVCYGCFIATYPFLITYYLIMDHKMFYGAFKDWFGALWAMRKKDSWISLWRDEARALDNFRDPHSRAMARYKEVPDWWFXAVLLLVIIVAICTIEEYHTSTPVWTLFMSLGFNFVFLIPLAILQATAGVSLGLNLLIEMIMGYALPGNPMALMIIKAFGYNIDGQADSYVSNLKLAHYCKIAPVALFRGQLIMAFIQIFINLGVINWSIDNFKEFCQPTQKAKFTCPDIQTYYNASVMWGGLGPTKIFNDVYPILKWCWLIGFLLGGAFGFAKKFGGRYYPTWFNPVIFMIGMLIGPPYGLMYYTPPLIMCFFSQWYAKKYHLRLWEKYNYVLAAAFNAGLVLSSIVIFFAVQYKDRYIDWWGNNVPYAGQDGLGEPLWNITATAKGYFGAAPGHYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0D1</accession>
    <name>A0A1E5R0D1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mitochondrial thiamine pyrophosphate carrier 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g4615</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0D1"/>
    <dbReference type="FunCoup" id="A0A1E5R0D1">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0D1"/>
    <dbReference type="InParanoid" id="A0A1E5R0D1"/>
    <dbReference type="OrthoDB" id="18574at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="30"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="148"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="256"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ80351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="362" mass="40396" checksum="9609DDCF2969AABB" modified="2017-01-18" version="1">MGTNANEDNHVRTNDTKNNSSDHLRSGSQISVLKSFIAGSCSGIAARTLTAPLDVLKIKSQLIPRTTATSSNQTRLLQLVKSVYQEGIYEYVPRRSCSKTTQSLLRQLHGLSCFYKGNVPGLYMYVIYGSVQFSSYSFFNSNFKFANNDSLQSMIVGALSGVTSSIVSYPCDVVRTRFVATLNQARTVQPSTTTTTTISANRLSMFNTLREIWLKENGIFGLFKGCSSSVVSVTMSTSIIFSVYESLKIYCETHENTQTLNAMSSSITGIVSKTVTFPLDTVRRRIQIMASKNINNYTREAGTYKEYTLHKNFVKIAYHMLQKEGWRTFYQGLTMGLLKSAPTTAITLSVYEFVLRKLMQGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0D7</accession>
    <name>A0A1E5R0D7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">25S rRNA adenine-N(1) methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">BMT2</name>
        <name evidence="6" type="ORF">AWRI3579_g4516</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N(1) position of an adenine present in helix 65 in 25S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the BMT2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0D7">
        <property type="interactions" value="110"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0D7"/>
    <dbReference type="InParanoid" id="A0A1E5R0D7"/>
    <dbReference type="OrthoDB" id="5954793at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016433">
        <property type="term" value="F:rRNA (adenine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03044">
        <property type="entry name" value="BMT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021867">
        <property type="entry name" value="Bmt2/SAMTOR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21008:SF1">
        <property type="entry name" value="25S RRNA (ADENINE(2142)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21008">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SENSOR UPSTREAM OF MTORC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11968">
        <property type="entry name" value="Bmt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4 6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ80362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="430" mass="49092" checksum="198F7EA790C6CC67" modified="2017-01-18" version="1">MALLGSKNSRTISGKTVTGRKVKPTVIKPSKTRKIIRRFHFLIKCRKTLINLLMDLSMQKTAQLCGCNELLGIQDADEEQNYRILTKFFQKNKPLWKKELVTSGNNDSKKDLEDKLLKLKSDTRPAALSGVDKTGKQESKCKEYVKLLQYITDEIHTNGGLQKYQLASIMGQDTKRGGDSSKFLIKWLNELNDEKKGLVDCENQLLYHNMRALEIGCLNANNYISRSPKLISSVERIDLNSNDTGLIKKQDFMERPLPGFIKKGANIRDGKILAKDKESQVFDLISCSMVLNFVPVDTKRGEMLKRIKLFLKDASTNISTGLPGGLLFLVLPLPCINNSRYMNDEFFVNSIMLKALNFRLLKRHHSHKIIYYLFEETDKKXNDIFIKRNLTKRSLTTSKASRYNITPSTSPGTLLEPKNDKNKNNFCILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0E1</accession>
    <name>A0A1E5R0E1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4469</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R0E1"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="273" mass="31703" checksum="94D9EACCD1A898B3" modified="2017-01-18" version="1">MRNVYTYGWSMKPQSNRSQKYXTXTRNSFKIKLNKTLTSTKVKFSKLKNKTKKNLESRKYAAVFGKSKKAKPSTCSKSDPFKVFSKPYQYNSDTFXPEVYRRNSSSTLGNDCTSVFFTDRFDCHSPYSLNTQSSYCTADSKCLIFKXERMNMAYTPTYTATAPTAXAPARPTPAFQEFNFNKKRXVFDNSTKDQRYFSNTGXTTXFPIKDQIKKYFSKPKIRSMVHRFKAEFISLMLMHAFGIIILTWLVVQRVKDYGRLGXDLLNEYAPIIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0K3</accession>
    <name>A0A1E5R0K3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinetochore-associated protein DSN1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4539</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0K3">
        <property type="interactions" value="470"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R0K3"/>
    <dbReference type="OrthoDB" id="3364649at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000444">
        <property type="term" value="C:MIS12/MIND type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013218">
        <property type="entry name" value="Dsn1/Mis13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14778">
        <property type="entry name" value="KINETOCHORE-ASSOCIATED PROTEIN DSN1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14778:SF2">
        <property type="entry name" value="KINETOCHORE-ASSOCIATED PROTEIN DSN1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08202">
        <property type="entry name" value="MIS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="61616" checksum="1324DC3D72A84E09" modified="2017-01-18" version="1">MMNETQTSRDLSAETFKRAKYQTQGYTNDESGFVYKKFKNQETNDNSVKNLNGRGAVLREAQVDDLRNINQAKWVDNFNSSYIPPPKSLVQPTMAPYTTIPLSPAYDINRKEALQLQGQQAFGAMPAYPQLQYGYPYSMGMPFVPSGNYPQQFMNPYYNDQQQLSQRIDNENSSSRSKGNEYGVDLEKEKLERRRKRELRESFVSQRGRRLSHISKPELVHQAEKRNGRDELPSSPSQNTQPATFFKHANVSIGKDLQVRQIVNWCLVKELDKMKEQVEKEKTAFNSNKITAKNIAYDIFKDFVNDAKSERPIKKWRGSRLQPSSRPDSKSNKHLGRPSKSTTEGYIDGHDNDGDALMDADIKAFLNSDLTNISPYKKSTTPGATKLQKSLSKKVFIPNLKNLQNRKMLEKLNTKIDILKKEEXVWKNCLAVNDKEIEKYIAQDEDGLREAMQMKRALKELPELNLHNDLMMRIDRFNETTHLINSNLELASXQQKEQLLKLSIRKDRDILDNRSNANNLSIKDYLCRLSGDSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0P8</accession>
    <name>A0A1E5R0P8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">assimilatory sulfite reductase (NADPH)</fullName>
            <ecNumber evidence="4">1.8.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g4548</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>hydrogen sulfide + 3 NADP(+) + 3 H2O = sulfite + 3 NADPH + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.2" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0P8"/>
    <dbReference type="FunCoup" id="A0A1E5R0P8">
        <property type="interactions" value="451"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0P8"/>
    <dbReference type="InParanoid" id="A0A1E5R0P8"/>
    <dbReference type="OrthoDB" id="1856718at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004783">
        <property type="term" value="F:sulfite reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06207">
        <property type="entry name" value="CyPoR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.990.10:FF:000010">
        <property type="entry name" value="Sulfite reductase [NADPH] flavoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000011">
        <property type="entry name" value="Sulfite reductase flavoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF109">
        <property type="entry name" value="SULFITE REDUCTASE [NADPH] FLAVOPROTEIN COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0288">FMN</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="14">
        <location>
            <begin position="654"/>
            <end position="885"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ80457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1041" mass="115531" checksum="FF6D5E6635C87394" modified="2017-01-18" version="1">MSSYQTNPFGIPTDSLDLPKYTTPSIGIQSVLFNHLESIFSYKTFSEPDLLDAGLKKWVAKNKEKVYFQELQIRAGAGSLPLGFQATDGNNSPVGIVTPGYGLKYLFSTLRKNAATNDTFKLALQVAALDYSNGVIKTDYVTPLRIAEALNFPVITPINHNEVKHTAAFSLFLSQYGSAVHLFDGANYAKTLASSKDEPSFTAIPPVNTIANAQKTDLDHFLSAFNDSSAVKIHNFKYFGSDNAKTVFVTYGSLESELFANYAENAEIGHIAIRIPFPFATDKFVALLPKHAENVVVIGQTLTPSSKSDLQKTVVSALKQFEKSNIQVSEFVYTPDFIWSPNAVAQIVESHVPTVASNSASTKDSSFVVWADDHNAENLDLSSKLVHALSLVDGQKISFRTKYDNVANAGTFQAQFISFNAKSQKIVSNVDNADVTFVANFNILNEFDVTNTTKENGTVIVVLDKKSIKDHDLNVLATFTEHLKISPQFLINLVKKNLRLVFIDKELVGDREETKTHTLSFVSQAVFWKYSIGADVAESVRRIWSSAGPDIELLASVISDSVTDAIANGVKEISTAQYADLFKDLATEKEVAEKEEPSAALKTFVEENSFAANPRSVPSTEENTSVVSPFEIAKQLTFSEAYGVKNDLRPHLPVKNFVVKVAKNKRVTPESYSRNIFEIEFDTTGTGLKYEIGEALGVHAQNNTTLVEEFLSKYNLNENDVVAVPNREDASILEYRTVFQVFTENLDIFGKPGKKFYESLIDFATNDDEKKQLTDLVSSAGASELKRFQEEEYYTYADILELFSSCKPKLEDLVKIIAPLKRREYSIASSQKVHPNAVHLLVVVVDWVDKKGNVRYGQASKYLSDLPVGAELVVSVKPSVMRLPPRPEQPIIMSGLGTGLAPFKAIIEEKVWQKEQGHKIGDIFLFLGSRHKKEEYLYGELWEAYKDAGVLTHIGAAFSRDQPHKIYIQDKINEALDKLSDAMCKEEGSFYLCGPTWPVPNITEALQNILAKDAAEKGVKIDLNQATENLKEVGRYILEVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0Q5</accession>
    <name>A0A1E5R0Q5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vacuolar protein sorting-associated protein 35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4391</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a role in vesicular protein sorting.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the VPS35 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0Q5"/>
    <dbReference type="FunCoup" id="A0A1E5R0Q5">
        <property type="interactions" value="1308"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0Q5"/>
    <dbReference type="InParanoid" id="A0A1E5R0Q5"/>
    <dbReference type="OrthoDB" id="10258141at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030906">
        <property type="term" value="C:retromer, cargo-selective complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.660">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 35, helical subcomplex Vps35-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005378">
        <property type="entry name" value="Vps35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042491">
        <property type="entry name" value="Vps35_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11099:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11099">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03635">
        <property type="entry name" value="Vps35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009375">
        <property type="entry name" value="Retromer_Vps35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="916" mass="106646" checksum="0D3F037B54CF0804" modified="2017-01-18" version="1">MYSNSLEQANGNIKQQTVLMARCLSQHKLMDAFKHASNMLSELRNPLLSPKQYYELYVTIYDTLTTLQQYLVENHKTKHHLTDLYELVQYAGNIIPRLYLMITVGSAYLYIEDSPKLAICKDMIEMCNGVQQPVRGLFLRYYLSQRTKEFFENGVIFIDHKSEENIDKIFKIDFITTNFIEMNKLWVRLQYQGPLKEKDLRTKERKELQVLIGSNLVRLSSILELSDVDLYTENVLPRILEQVVQCRDIIAQEYLLEIILKIFPEEFQISGLTKILECSLKLTPKTSISKIIITLIERMILFKKRSADYTDSSVFNKIWSYLVTLDEERPDLPIKEFILIISSLTQLVQLWDAPHPYINLDSLYKFIFYKKDDYTITEETNNMLKKLLLISSSSTNDFFGENIEFDAAKTTDDLYFNLITKCEYYVELLVSLSNEQLKIDTIETIFHTIFDNSNKKTSKYKISNKDHLIQFTKMLQPCLLENCELVSKFFHLIKNKNLQTQFDLLNYCHNWIISTNSNRGYILESLIVEYWKLIRKYCKKINTKSPLQKSKKKNETLTKLHVEKIDSATFIKQFFKTIGRCIQEIPNAEIQFKMNCTTASLADQLKLKDLSYDFFVNAFINFEEMSDSQLQYQSIVYISQCLQKTRSLFAFEKNGENKSLSTAQNESNYYENLITKCTLYGSKLLNKQDQCRSVLQCSHLWWCTEVPALGEEEGVTGSFFRDGKRVLECLQRSLRVADSVMDNCTSFLLTVEILNRVLYYFIHGETSYVTIKYCNGLIELIKTNLQFLERETRMATTSKNEIENSVLNHQADSNSALPSTSSSAEHHANFLTCYGIGIDGTIIKSFNKQESSKLSDVLFGKRLHQNEHDAIDKASAQKSFASIAKNNRIPIEFFERTLNYIKLQRTVDDRFKLIAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0R5</accession>
    <name>A0A1E5R0R5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">U3 small nucleolar RNA-associated protein 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4606</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
            <location evidence="1 5">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the NRAP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0R5">
        <property type="interactions" value="1094"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0R5"/>
    <dbReference type="InParanoid" id="A0A1E5R0R5"/>
    <dbReference type="OrthoDB" id="10251401at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032545">
        <property type="term" value="C:CURI complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006409">
        <property type="term" value="P:tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005554">
        <property type="entry name" value="NOL6/Upt22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035082">
        <property type="entry name" value="Nrap_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035367">
        <property type="entry name" value="Nrap_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035368">
        <property type="entry name" value="Nrap_D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035369">
        <property type="entry name" value="Nrap_D4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035370">
        <property type="entry name" value="Nrap_D5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035371">
        <property type="entry name" value="Nrap_D6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17972:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17972">
        <property type="entry name" value="NUCLEOLAR RNA-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03813">
        <property type="entry name" value="Nrap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17403">
        <property type="entry name" value="Nrap_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17404">
        <property type="entry name" value="Nrap_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17405">
        <property type="entry name" value="Nrap_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17406">
        <property type="entry name" value="Nrap_D5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17407">
        <property type="entry name" value="Nrap_D6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Nrap protein" evidence="7">
        <location>
            <begin position="213"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="8">
        <location>
            <begin position="371"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="9">
        <location>
            <begin position="538"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="10">
        <location>
            <begin position="720"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="11">
        <location>
            <begin position="922"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="12">
        <location>
            <begin position="1104"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ80486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1245" mass="141399" checksum="CB10FBF7DA166AF9" modified="2017-01-18" version="1">MQIISNKRKLSEVTNSSSNSSSNSNSNGTTEGASISKQAKLNTHKTDAVEEKVVSEQHKEEEKEEEEADEEEEDSDGDEDEQSAHANTKVQLTSQDIQIARETVELFKSNVFKLQIDELLEQVQLKESSIIKVERFLHKLYDLIQEIPEWGVDKSLEEVESFFKDKIVSVPFADPKPQASTTKFKFGYLKPTVSLVGSFALKSAIYQPNGSCIDVALQMPSVLFDKKDYLNFRAFHKKSVYLAYLTHHLNILFEKYNMDNFIQLHYKYLHNDSLQTVLQISCSNPKTDNQTSSSPLTEKLNFFKTKFKINLIVTLPENVFDNKKLLPHKNCIRVQNDNTTPTPLYNFALLASTTSEHYLKYLHKSKKMTEAYKEASVLGRLWLHQRGFSSNISQSGSLAGFGSFEFCVLMSALLNGGGENGNKILLHGFSSYQLFKGIIKYLATMDLCSTGYLQFQSFADQSDKNSQSSNTNSKYVKEGFNVPTIFDKSTKINVLSKLTPSAYAILKLYAQETLQMLDNVVKDQFENIFLINLQKSNILKYDYEIDLSLSNTLSTLYEGFDPKERVTSMTFENYVVKKISQLIETALADRINGXELDVISESKASDXDSKRSSLFPIXKRRLNKKVLGLKIKLLVNPMESEKLVTRGPTNEESAEKIALFKKFWGPKASLRRFKDGTIVHSCVWSSSSSQSVVLTILKYILKQQLHGDDNMFHAIXMXKFQKLLPLPNVPGSATMSILNLNNYHALKKSFDELNKIIFQMSLPLSVKSVFPIGNAFRYTSLCQPVPFAYSNPDFLQDVVLEFENSAKWPDEISSLEKAKTAFLLKIHDKXSESYAEYSSYFTRDESIPNNLDIITLNILTPEGFGFRFRVLTERDEVLYLRAIGNARNELKPELERTFFKFSAKYQAAIRHSRTLEMISHSYPLYSPTVRLFKKWLDSHLLLSHFPEELVELLAIKPFVENDPFHVPGSLQNGFMKVLKFLSSWNWLEDPLIMDLVKNESFTDMDGANNEISSTTLKNLSERLTFAQYKDIQSNFETMRKSDPNGLNIQFFVASKNDPSGILYTNGIPLAISTRLTALAKVAMNFVSVHGLNEKTINLLFSPGLKDYDFVCKIKLEESLKLSSGCAGEFKNLDQTATKFPSNVNDLSTKMDPTHHLVKYLNKKYSNFIIFSSHKYVAATSENGDVNVITGLIKPFFKSNQQKFRVNMDCDVKPVDEQNVQLNKEAVLNEIAIFGGDLITEIQVNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0X2</accession>
    <name>A0A1E5R0X2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g4492</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0X2"/>
    <dbReference type="InParanoid" id="A0A1E5R0X2"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ80541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="410" mass="45438" checksum="58A6B044398D5EC2" modified="2017-01-18" version="1">MQQPSNNFMQASTKANLPVLPIPHAKLFKKPVNPGSATIRPGNHGLMELFAEKDRVDSESILSRAVSYSEIASLDVLEGNDLLTSDSLCTPDKSFPVSGTNSFSKTAVFKSKMANVSIGKKVKKFFKRMNPFGSAGTAKQQRVSLFKKQKVARGDTIIIVSSFKLKPFAPPPTCMKNVSETTSNAPATPATPATPDNPFTLATFIAVFRARQLEFAKAYLFGNKSISTVERMLDNHKGELIKHEEKLASGKLRPLLLTDCRFAHTHGTDTTSSFVPKTPCEWACVYNQADHQLARIRCKRTMAEIERSREKAVANGAVDNAKIAEIWSQGVSRCTKEISDKADAMVKFLQPNVSERLDLDCYIRNMKNPTPSSLPDNVFADEEYRLLLTKIHFIEYVIAEKQKAMDSNPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0X3</accession>
    <name>A0A1E5R0X3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">1,3-beta-glucanosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4529</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Lipid-anchor</topology>
            <topology evidence="11">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the glycosyl hydrolase 72 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0X3">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0X3"/>
    <dbReference type="InParanoid" id="A0A1E5R0X3"/>
    <dbReference type="OrthoDB" id="421038at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042124">
        <property type="term" value="F:1,3-beta-glucanosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071970">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004886">
        <property type="entry name" value="Glucanosyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468:SF5">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03198">
        <property type="entry name" value="Glyco_hydro_72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 11" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9 11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0732">Signal</keyword>
    <keyword evidence="5 11 13" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009028093" description="1,3-beta-glucanosyltransferase" evidence="11">
        <location>
            <begin position="21"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="566"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="566"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="580"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ80554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="663" mass="70825" checksum="381B4315968CEECE" modified="2017-01-18" version="1">MQLVFSXAXWLFVYVKFTSAYITTHGNAFVDSDTGKDFIIRGVAYQPGGSSSVGDCLSDTFALQRDIKYFQQLGINALRVYSIDNSLDHSAGMQMLKEAGIYLILDINTPGASINRPDAFCSYNSAYLQNVFAILDEFTHYDNVLGFFAGNEVIDATSNTDLAYYIKAVVRDIKNYMKARNLRKVPIGYSAVDISNQIDTLDYLTCSDNESENIDMFGVNSYSWCGESTLQTSGYXVKHAEYSASTVPVFFSEFGCNVGTGGAKNRPFTEIGALYTDDMCGVFSGGVVYEYSEEPNDFGLVKINADDSVTPMENFYNLETQYSAYPNPTSCDFTTGNTRPQCPAYKEGTWDVQNTSLPAMPDSAQGYFANGAGKGLGLSLDSRYMCDESNESGGWKKGGTESFSVSLKQATSTFNVVSTHSSSSTSFASGMSGTSSSGVSTMSSAPVTLTSSTSFSTALISTLASTLTSESSSLPEPPSTTLTSTSSSSLATMLQTLTSSSSSITGSIESFAASAGSYLRAATSSTLSSSISIVSSVSGPSSFSLTSSTPVLHTIVIPTSSFHPSISNSTSMRSFGPDQSTSTRMSLSPSSEASSSSAYTETSTLRNSSSESSTTLSKTSSSPKSSYSEENSSSRKSQGNAHLHRSSTHWLSILTVWFFLLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R185</accession>
    <name>A0A1E5R185_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g4196</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R185"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048744">
        <property type="entry name" value="MCM16"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20993">
        <property type="entry name" value="CENPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ80669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="202" mass="23645" checksum="7AA2C467C2A93C74" modified="2017-01-18" version="1">MDGLTFNKAISDLEEEYTQTCYQLIKNLQTISSRQQSHQYTSEEEEQQQMDVITSLQMNLVMTLPVMKALCSNEQGEERKNLNTDRYGDIISRRQSTKELENQNNNDFDEYLRELMSLSYECDLDLMKRIDSYSESVLTPSERAKHFKKAGVSSREHLIKNIKNKLKTLHNKDAKRDTKTRRIVVLRELINXFEANLGRTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1H4</accession>
    <name>A0A1E5R1H4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Invertase 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3970</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the glycosyl hydrolase 32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1H4">
        <property type="interactions" value="450"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1H4"/>
    <dbReference type="InParanoid" id="A0A1E5R1H4"/>
    <dbReference type="OrthoDB" id="202537at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004575">
        <property type="term" value="F:sucrose alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005987">
        <property type="term" value="P:sucrose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18622">
        <property type="entry name" value="GH32_Inu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.560">
        <property type="entry name" value="Exo-inulinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="entry name" value="Glycosyl hydrolase domain, family 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001362">
        <property type="entry name" value="Glyco_hydro_32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018053">
        <property type="entry name" value="Glyco_hydro_32_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013189">
        <property type="entry name" value="Glyco_hydro_32_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013148">
        <property type="entry name" value="Glyco_hydro_32_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42800">
        <property type="entry name" value="EXOINULINASE INUD (AFU_ORTHOLOGUE AFUA_5G00480)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42800:SF4">
        <property type="entry name" value="INVERTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08244">
        <property type="entry name" value="Glyco_hydro_32C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00251">
        <property type="entry name" value="Glyco_hydro_32N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00640">
        <property type="entry name" value="Glyco_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00609">
        <property type="entry name" value="GLYCOSYL_HYDROL_F32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 32 N-terminal" evidence="7">
        <location>
            <begin position="12"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 32 C-terminal" evidence="8">
        <location>
            <begin position="349"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="496" mass="56773" checksum="24FDD3168D2AB661" modified="2017-01-18" version="1">MSLVSSKRPSLHLTPLKGWMNDPNGLWYDQSTGYYHVYYQYSPDLHWSLPITWGHATTNDFVHWEHKQDKLLASRAGEGAFSGSVVVDKYNTSGKFDSIADDNVVSIFTLHTPDRQTQNVTFSKCDLPLSADDSTVVLDINSKQFRDPKVFWHEETEKWIMVVARTQVYEISLYSSKNLVDWKYLSGLKKHGILGYQYECPNLVKIPVXDPVAKEKIGYKWVMFVSINPGGPIGGSFVEYFVGEFNGAEFIPDTNEARIMDHGKDFYAVQVFNNTESEDLVLALGWASNWQYSEMTPDYAWKNCMSIPRKLTLQAHRANDDSLEHLVLYNEPFFGNGAEKRKTTXALHDKSITKTAXVFKFKIKWSFKPQRLARGTFTAWSIKVCAPNSESSLIIGFEACSASFFIDRGVLFNQPENYLFNNRSTVDLVPYKIDSHGSQHFSVEGIVDRNILELFFNDGYATATNTFFFECSDFSDIHCSRNEVFDYLDMEKTEYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1R0</accession>
    <name>A0A1E5R1R0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Succinate-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="8">1.2.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g4500</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 8">
            <text>succinate semialdehyde + NAD(+) + H2O = succinate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13217"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 8">
            <text>succinate semialdehyde + NADP(+) + H2O = succinate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13213"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Amino-acid degradation; 4-aminobutanoate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.16" evidence="8"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1R0"/>
    <dbReference type="FunCoup" id="A0A1E5R1R0">
        <property type="interactions" value="451"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1R0"/>
    <dbReference type="InParanoid" id="A0A1E5R1R0"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="UniPathway" id="UPA00733"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004777">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036243">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07103">
        <property type="entry name" value="ALDH_F5_SSADH_GabD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000004">
        <property type="entry name" value="Succinate-semialdehyde dehydrogenase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000005">
        <property type="entry name" value="Succinate-semialdehyde dehydrogenase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050740">
        <property type="entry name" value="Aldehyde_DH_Superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010102">
        <property type="entry name" value="Succ_semiAld_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01780">
        <property type="entry name" value="SSADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353:SF5">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="9">
        <location>
            <begin position="29"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00050387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00052698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="504" mass="55072" checksum="BF4D858D57844E96" modified="2017-01-18" version="1">MSTFEYTDIKKLLKNPELIRTTSFVNGQWTKQSDSTASFKVTDPGSQDNKVIAELPDEPLSIVDFAIESAYDSFKKYKNTTPRQRASWLRTLYNLMMENAEDLAVLVTLENGKSFSDALGEIKYGATYFEWFAEEAPRLYGHTIQPNNPKNRVFTRKEPVGVVGIICPWNFPSAMITRKAAAALAVGCTVVVKPDSQTPLSALALAYLVEKAGFPKGSYNTVLSHTQTPEFGLKLCEDKRVKKITFTGSTNVGKILMKQAASSMSKVSFELGGNAPFIVFEDCDLKQTVDQAIAAKFRGLGQTCVCANKFYVQKSLVPKFTEALKEQVLGFNIGHGLNKSSTHGSLINDKALEKTKDHVKDAVENHGAEVVIKGGPLPELGPNFYSPTVLTNVSPKSKVCQEETFGPLCAIVPFETLEEVVAYSNDCDVGLASYVFSKNIDTIFQVSEALESGMVSCNTGLFSDCAIPFGGVKESGFGREGSLYGVDDYTVIKTITIGNLRDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1S5</accession>
    <name>A0A1E5R1S5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4180</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1S5">
        <property type="interactions" value="124"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1S5"/>
    <dbReference type="InParanoid" id="A0A1E5R1S5"/>
    <dbReference type="OrthoDB" id="67566at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021149">
        <property type="entry name" value="OligosaccharylTrfase_OST3/OST6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12692:SF3">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT OST6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12692">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04756">
        <property type="entry name" value="OST3_OST6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184593" evidence="6">
        <location>
            <begin position="24"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="317"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="352"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="382" mass="43772" checksum="18B091E834D3E3DF" modified="2017-01-18" version="1">MQYQLFTNFWLXILLAIGSNVQGLLSQVQDATNLDYLSQKPQQPVLKFPYASLADEDGIITIDSTELYNTIVNSTFTITNHYTLLYLTSSESEEGDFFEHRVLRKISNFVNKQYNHVNGNDGNDGTGDENDFENPLRMVFIKLVKENLPEVFDIDLQGRVESENLPIVLVYAPTVSSNQQDNKEPAFFNYLTNTFYKYAVTKDFQQSIGSINKEHYKFAEFLSTLANMLLKVDEEFDTEKFAKQFACALIVFVIFKKKIYPLLKDHFLHKILLILSLVAILVCCTGYSFVKMNQIVLLAHDDSGQIMWFSGGFRWQFGIEIFTVSGMYLLLAMLTYLLVYVSKTVETDSVHCISALALVTILVMTSQYLVSCFKIKYPDYMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R261</accession>
    <name>A0A1E5R261_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g4009</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R261"/>
    <dbReference type="FunCoup" id="A0A1E5R261">
        <property type="interactions" value="314"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R261"/>
    <dbReference type="InParanoid" id="A0A1E5R261"/>
    <dbReference type="OrthoDB" id="3973165at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.10">
        <property type="entry name" value="Mediator complex, subunit Med21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037212">
        <property type="entry name" value="Med7/Med21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021384">
        <property type="entry name" value="Mediator_Med21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13381:SF0">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13381">
        <property type="entry name" value="RNA POLYMERASE II HOLOENZYME COMPONENT SRB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11221">
        <property type="entry name" value="Med21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140718">
        <property type="entry name" value="Mediator hinge subcomplex-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="73"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="159"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="80"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ80985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="219" mass="25210" checksum="B67047BF8F613734" modified="2017-01-18" version="1">MQRIFFKPYQGKAEGTSRSRQSFFQMNSVPSPTTPKSKDRLTQLQICFDQMMEQFCATLNYVDKNHILNEQPQDPEQHLQQQQQQQQQQQQQKSPSGDAAAEKTVQDGAENSTKSSTENENFNHEEFEGTIHELSNDLILKTRQILKLIDSLPGIDASSDEQMMKIDRLSKELMEVEQEKLQVIKEKDNILMTVTDLIDDFTSNKKTINTAVPISNERQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2N1</accession>
    <name>A0A1E5R2N1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Elongation factor 1-gamma 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3921</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2N1">
        <property type="interactions" value="938"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2N1"/>
    <dbReference type="InParanoid" id="A0A1E5R2N1"/>
    <dbReference type="OrthoDB" id="249703at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03181">
        <property type="entry name" value="GST_C_EF1Bgamma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1010:FF:000001">
        <property type="entry name" value="Elongation factor 1-gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1010">
        <property type="entry name" value="Translation elongation factor EF1B, gamma chain, conserved domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050802">
        <property type="entry name" value="EF-GSTs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001662">
        <property type="entry name" value="EF1B_G_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036433">
        <property type="entry name" value="EF1B_G_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986:SF1">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00647">
        <property type="entry name" value="EF1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01183">
        <property type="entry name" value="EF1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89942">
        <property type="entry name" value="eEF1-gamma domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50040">
        <property type="entry name" value="EF1G_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3 8" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="2 3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="7">
        <location>
            <begin position="109"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="EF-1-gamma C-terminal" evidence="5">
        <location>
            <begin position="279"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="233"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ81165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="440" mass="51045" checksum="90B01C0C19FF6C00" modified="2017-01-18" version="1">MSQGILYANQRIRSWIPRAICDYFKLDYEXVNPEAITDQNDKFFKDFPLKKIPSFIEYQNDAHPNAVFKLTEVIAVMYYLLEPLDDEYKKTGDPQYQLAHQLINGRTGSYTTRATILRWISLVNSDFLITLAAVMKALRGELPYIKKDFDAKSAAVDGMVADIFEKRLSEYTYLADEIPTLADFFACVVFTRGFNFVWGYEWMAKYPATLRWFNTVRDHPFLKPFYADFHFNEKPLSPPQPKKKESKKKDASQEKQQQVLAAKKXEXPAAEAPAAEKKPKHPLELLGKSXFNLDEWKREYSNNDTRPVALPWFWEHYNPEEYSIWKVSYKYNDELTLTFMSNNLVGGFINRLSGSIKYMFGCLVVYGENNNNGIIGAVMIRGQDFKPAFDVAPDWESYDYEKLDVSTEENKEFVNNMWAWDKPVVINGESKEIADGKVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R3G7</accession>
    <name>A0A1E5R3G7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Proteasome chaperone 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3929</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R3G7">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R3G7"/>
    <dbReference type="InParanoid" id="A0A1E5R3G7"/>
    <dbReference type="OrthoDB" id="3980246at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053720">
        <property type="entry name" value="Psm_Assembly_Chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018854">
        <property type="entry name" value="Psome_chaperone_3/4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10448">
        <property type="entry name" value="POC3_POC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0647">Proteasome</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ81113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="151" mass="17049" checksum="97CBD01B3FDEA38B" modified="2017-01-18" version="1">MQYRETLVKTANNAEIHICCQFFANRVLVQIRWNGELDTSYSVTCQDITQVLEEXHNYGEDGEATDGXDGFYQDPLSNYQITTLFGDSNNLELPIIASQVSELFIKNKDRVPNIGSFVVMLSSKLFHAQDNTKFDTLLTILAAIKQDIIAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4R0</accession>
    <name>A0A1E5R4R0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="13">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g3575</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. RAD25/XPB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="13"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4R0"/>
    <dbReference type="FunCoup" id="A0A1E5R4R0">
        <property type="interactions" value="1029"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4R0"/>
    <dbReference type="InParanoid" id="A0A1E5R4R0"/>
    <dbReference type="OrthoDB" id="10262986at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000112">
        <property type="term" value="C:nucleotide-excision repair factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18029">
        <property type="entry name" value="DEXHc_XPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18789">
        <property type="entry name" value="SF2_C_XPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000077">
        <property type="entry name" value="Probable DNA repair helicase RAD25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000117">
        <property type="entry name" value="Putative DNA repair helicase rad25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050615">
        <property type="entry name" value="ATP-dep_DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032438">
        <property type="entry name" value="ERCC3_RAD25_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001161">
        <property type="entry name" value="XPB/Ssl2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032830">
        <property type="entry name" value="XPB/Ssl2_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00603">
        <property type="entry name" value="rad25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274">
        <property type="entry name" value="RAD25/XP-B DNA REPAIR HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16203">
        <property type="entry name" value="ERCC3_RAD25_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13625">
        <property type="entry name" value="Helicase_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00851">
        <property type="entry name" value="XRODRMPGMNTB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 18" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="346"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="562"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="25"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ81870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="812" mass="92632" checksum="4E88F453FEAA93AA" modified="2017-01-18" version="1">MAEVEEDKPLISRRRKNTIFQQQLNEEEVSDATDYSEEEDNAASQKPLAKPKKNSKKQEEINKDNFVQEENDIPEDFVPDVVSDIFRNQDFSYLHLKPDHALRPLWISPSDGRIILESFSPLAEQAQDFLVTIAEPISRPSHMHEYRITAYSLYAAVSVGLEPDDIISVLDRLSKVPVAQSIINFIKGATVSYGKVKLVIKHNRYFVETTQTDILQMLLRDPVIGKLRIDNTDGAKNEDNLTGVKNEKTTNELLGDKHSNINKDDVEAVFSSVVGEEDLDNDDDAVHLFEIANDSVEVVKKRCQEIDYPVLEEYDFRNDNRNPDLNIDLKPSTQIRPYQEKSLSKMFGNGRARSGIIVLPCGAGKTLVGITAACTIKKSVIVLCTSSVSVMQWRQQFLQWCTLQPENCAVFTSDNKEMFQTESGLVVSTYSMVANTRNRSHDSQKVMDFLTGREWGFIILDEVHVVPAAMFRRVVSCIAAHAKLGLTATLVREDDKISDLNFLIGPKLYEANWMELSQKGHIANVQCAEVWCPMTAEFYQEYLRETARKRMLLYIMNPTKFQAAQFLIQYHESRGDKIIVFSDNVYALQEYALKLGKPFIYGSTPQNERMKILQNFQYNDKINTIFLSKVGDTSIDLPEATCLIQISSHYGSRRQEAQRLGRILRAKRRNDEGFNAFFYSLVSKDTQEMYYSTKRQAFLVDQGYAFKVITHLHGMENLPNLAYATARERRELLQEVLLKNEEVAGLEVGEDSDNVIMNKRMRKFNSVKNEGSLAGLAGGEDMAYMEYSSSADRDGSKHHPLIRKMYYKQPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4R7</accession>
    <name>A0A1E5R4R7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">EKC/KEOPS complex subunit PCC1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3566</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CTAG/PCC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4R7"/>
    <dbReference type="FunCoup" id="A0A1E5R4R7">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R4R7"/>
    <dbReference type="OrthoDB" id="10025739at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015419">
        <property type="entry name" value="CTAG/Pcc1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09341">
        <property type="entry name" value="Pcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="93" mass="10480" checksum="58538226298F8B36" modified="2017-01-18" version="1">MSQDLQHTLKLSIPLESSKQTDILIQSMSPDPVLNPGQFSVEYTTVNSKNNNTYGVDITFKSIDERVMRVGVNNTIEALKSIIETFDEFDNNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4V1</accession>
    <name>A0A1E5R4V1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Chitin synthase</fullName>
            <ecNumber evidence="2 7">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3783</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Polymerizes chitin, a structural polymer of the cell wall and septum, by transferring the sugar moiety of UDP-GlcNAc to the non-reducing end of the growing chitin polymer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n) + UDP-N-acetyl-alpha-D-glucosamine = [(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9593"/>
            <dbReference type="Rhea" id="RHEA-COMP:9595"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17029"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="EC" id="2.4.1.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the chitin synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2 7"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4V1">
        <property type="interactions" value="102"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4V1"/>
    <dbReference type="InParanoid" id="A0A1E5R4V1"/>
    <dbReference type="OrthoDB" id="26569at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013616">
        <property type="entry name" value="Chitin_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF38">
        <property type="entry name" value="CHITIN SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01644">
        <property type="entry name" value="Chitin_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08407">
        <property type="entry name" value="Chitin_synth_1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="625"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="657"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="689"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="725"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="760"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="858"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="890"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin synthase N-terminal" evidence="9">
        <location>
            <begin position="217"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ81939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="953" mass="109581" checksum="564A5AC26F7E9A82" modified="2017-01-18" version="1">MKNPFLDTRTALRSPERQPYPQDGYSEASSLGQXNVYPDFTPPASPSKAALRYSPKRKHLLPISAHMPRHEATRMESPKRKDRVVIDIDSDSDDDNNNNNNNNNIESDFFDVHQSPSKARPQPPTIQISNTFEDPFNEQKDIEKFDLASEISTTYSRQVSTTDISSMSKSIDENSTIYSGDTFEHTRFQQHHHDLKYIRRGNSTARPRPSKKKASKILTLDNPIPRGILDVLPNRSSPEFTEMRYTACTTDPNDFVEEGYNLRFADMNRECQIAVCVTMYNEDKYCLARTMHSIMRNVSYLCKREKSRVWGPNGWKKVQVIIVSDGRSKIDRGSLDYLISLGAYQEDMAKNTVNGQPVQAHIFELTTQISIDETLNYVSKDIVPVQVVFCLKEENQKKINSHRWLFNGFCPVLDPTVVALVDVGTKLNDSALYHMWKAFDRDSNVAGVAGQIKAMKGKYGSKLLNPLVASQNFEYKMSNILDKPLESCFGYISVLPGALSAYRYRALQNHEDGTGPLHSYFLGEKQETAQSDVFTANMYLAEDRILCWELVAKRNARWILKYVKEATGETDVPEEPPEFISQRRRWLNGAMFAALYSQLHFYQIWNTRHSKTRKFFFHVEFFYQFVQFVFSWFSIANFFLTFYFLAGSMTMLIKNGMVLFTFFKYLLICTICGLFIISMGNRPQGANHLFITSMVILSVCSTFAIITGLYFSISTLKKNEETHAAFVNIVVSMLSTYGLYAFTSVIYLDPWHILHSTVQYFLMIPTYICTLQIFAFCNTHDVSWGTKGSTKEDKPKSEAVIRAGPDGKTVITGTEWPQDIDKKYIEIRNRLKDNEIVESKPDPVQKNNDYYRDIRTRIVMFWMLSNLILCMIITQVYGPKDMYKNKYLVFVLWSVAALSLFRAFFSVLFLVLQYTRNIISYKHKIQRSGARVSGGSNIKSWFKSEEKIKGIAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4X8</accession>
    <name>A0A1E5R4X8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3787</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R4X8"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="111" mass="12268" checksum="23E1A88B61C6FFD6" modified="2017-01-18" version="1">MSTVEEQVAATTATTTEQVAQAPTEQVAETPXVQDATTPAEVEQVLEQNASEVAQGGDEEVKQTQENVEKPVEEQAKEQAEEPVKEQESGNLKTKVNNMFKKYLKKFKTVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R578</accession>
    <name>A0A1E5R578_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein NBA1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3712</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R578">
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    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R578"/>
    <dbReference type="InParanoid" id="A0A1E5R578"/>
    <dbReference type="OrthoDB" id="4067583at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="440"/>
        </location>
    </feature>
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        <location>
            <begin position="458"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="355"/>
        </location>
    </feature>
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        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
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        <location>
            <begin position="394"/>
            <end position="403"/>
        </location>
    </feature>
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        <location>
            <begin position="412"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="495"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="609" mass="67502" checksum="AC963EA480C1787A" modified="2017-01-18" version="1">MPPASNNSVSNYEKLSKMINSMDLSSNSIDPDQSLNDTVNDNSPTDSKTRASVGSNHTKQQEVESFGSYDGKVQEVVPVSYIVTKSSSEMTSNRKNSVLERQSEIIVIDEEDTDDENVIKETGTDNENDEHEYEAKNMNQKFSSYGSLPKASPQGVKREASSFKLSVPNKTHKSAPSSVVSGQVASVSDYSIHTPMMSEYSFMSSTQTGKPVRRLQIDRKPTIKSIVSDPSSAEPDFTVDTNYYRTQNRINFSIKPDRSEDTEIKPSIPARSRDRPRSGIYSLGSPTGIKSASSPISKNGSPRSKRESLDQINLQLGEQMEELRSIVSEQDDKVDGKADTHGARNNAQTATQSTIKMKENDSPNSRSQEAFQFANEINLSGQPXITKLQKESSKPSPSTGSSXNLEEDDFYSATSDFPNENGTSPVTHTDPHEARKNGAGAFHRSSLDDSYLQRPVPELPQSKQVKDENLINIPEDQYEDIEGTTHSPKKLPRQMSRQSRNSIRTGKSKSSMGVPFSNASIFSKRTGERKKEIRSMFDIDTLAQLLNVTKGTMIGSEFANLGMKSEEKIALEKLIDSLSRLTSDMILDPERYEEGLKRLKKATRALEGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5A7</accession>
    <name>A0A1E5R5A7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoinositide 5-phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3678</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptojanin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the central section; belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.36" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5A7">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5A7"/>
    <dbReference type="InParanoid" id="A0A1E5R5A7"/>
    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043813">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000029">
        <property type="entry name" value="Inositol polyphosphate 5-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF269">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE 5-PHOSPHATASE INP51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SAC" evidence="11">
        <location>
            <begin position="213"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="29"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1061"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1076"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1101"/>
            <end position="1126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ82072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1126" mass="127903" checksum="91931EBB557AFD24" modified="2017-01-18" version="1">MKLYLNTKVRQIALVANDYALVFKTTNXSSTKSGQNKSNTRGLNNEGSSDETSQAGEKSKSEDENGSHLASKKSVVVESFHFKELKSRGFKLVETLLFEGFLGLVNVGEQIFLGLIEASQQAAHPRWTWDNERHLIQPIEAINQIVSVSFVCLTSNRYDDQLFDPAGLNHARTSNSFDRLYTNVNYAGHPNGSNLGEEGSNYNNYASHPCAELKKLFCDGTFYFSREFDLTNNLQERGIISSMDYIIEKFDEKYVWNINMIHEMIQFRSRISSQEKEYLDKGKFITFVIRGFCKSKISELQLANPTCVTIITKISTENKRHLLAVGGIDNEGNVSNFCESEVILSTESHIFSYVICYGNVPLSWEMQESQILHSKKIKLSKSPEYTQASFDKHFDRLATKYGIVSIVNLVKPKSHSQAALASAYQKCAESKGIRFTNIEYSSDVLTRFTHKLLYHMKEDILDFGALVYDIERGIYCGKQTGVIRISAFNSLKRPFLVEKSVSQEVIEMAVEELGAFQIPADFSHMYEKVWKEQIFFMNKLYEENFKHRDRYQKVYRSLFSLKVTLYDPLHSYIVNYLRKRKKEFLYDRTIKIFSGTFNVGGTLYKGDISSWLFPKEHLKTNSPDIYVIGLEEVVELTPGQMLSTDTLVKPFWENHVLKTLNEHSKSKRYVYLWGLQLGGILMMVFISEPEYSNVKKLEGDFTKLGFGGISANKGAVAVRFVFSTTKFCFIASHLAAGLQNVEQRHNDYKTVNKNICFAKGVTIKDHDGVIWMGDFNYRLLLSNEEARAMIKEKRFEQMFEMDQLNQQMISGESFPYYNEMEINFPPTYKFDPGTKSYDTSEKNRIPAWTDRVLNRGDILTQLEYGCCENIVFSDHRPVYAVFNADVTLANEPLKMKLANEIYQTLQKELIDLDIQEKFDVLQAAEDVVSSAANKKAAFIDKDLSMASVNKANRLGYEYESIDANNATFAKNNNSGSTRSSXAGMGDNLVPCGKGLNIKSYTGNTKLAPPSTDKNKWWIGDGKQATIILDINPKDYMINPHRSTNPFELDSSEPFFAPRVENEDELEPVPEHESAQIKPNSGLPSSVPKNLQDLAVQNSVAEKGKLDSTLKGADTGKLKTEDTPQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5B7</accession>
    <name>A0A1E5R5B7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3599</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PDCD5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5B7"/>
    <dbReference type="InParanoid" id="A0A1E5R5B7"/>
    <dbReference type="OrthoDB" id="10252486at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.140">
        <property type="entry name" value="PDCD5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002836">
        <property type="entry name" value="PDCD5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036883">
        <property type="entry name" value="PDCD5-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840:SF0">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01984">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46950">
        <property type="entry name" value="Double-stranded DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="151"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="156"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="201" mass="21944" checksum="512EA351DF5DC7D4" modified="2017-01-18" version="1">MNSNTNHKQQSPAAVSTDAVSHGGGAGGAAPQPKINEMIIPLLDSSAYQRLTRILMVRPERVRQVEQYVIMLAQQGQVNQKNKLTDTELKGILDGLSRTENVQNAEKKSNDGNHSNSIKNNGAFTQGITFNRRGGFMDDSDDDLDFLITNNGSGYTTTTXNTTTTTTTTNTNTNTTTRNNNNNFNNYSANAADDSDDDFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5E7</accession>
    <name>A0A1E5R5E7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3809</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R5E7"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="268" mass="31632" checksum="54A9DF0C75615DAB" modified="2017-01-18" version="1">MLPRHVIGYDSAARHFHLISHRVLSDQNRFFKYLLYGIPRCLYDLNLFTFEIISVEDFSHPFEDMSNPIKYVCYLGFPFDSFAKNYHXFSEVITSNGCQNFISVYYVKQKCPYVQRFEVWKKLVINWAEVDLPYCFYHKHSNNDKFADYLTFLYNGHHYDFTMQGQQRNIYSSLVKPFYKCRKKVRKWKFAYCNNALIQGAYRIGLKPYQSTCIKITDGSEPEHTDXGEAKSRSSGPSXEETTLLETNLQDYLSALKESFEFMQAKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5E8</accession>
    <name>A0A1E5R5E8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Metal homeostasis factor ATX2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3583</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5E8">
        <property type="interactions" value="296"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5E8"/>
    <dbReference type="InParanoid" id="A0A1E5R5E8"/>
    <dbReference type="OrthoDB" id="3970968at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006829">
        <property type="term" value="P:zinc ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045891">
        <property type="entry name" value="ZIP9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16133">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 39 ZINC TRANSPORTER , MEMBER 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16133:SF0">
        <property type="entry name" value="ZINC_IRON REGULATED TRANSPORTER-RELATED PROTEIN 102B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="315"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ82121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="334" mass="36536" checksum="03E7279F6F73D867" modified="2017-01-18" version="1">MGFLKAVFELLVTAGIFHFATFAIGMIPLIYFEKWRELKRQQTDTAYNSELNRTRGILNRCAEFVFKYMSSFGMGMLLGTSFMLVIPEGVYSVLDSRGNIGFNLLFGFIVVYLLDSLLVHKMVGSSSEFDSVSVQSQFEESNDYPGSVXSAGVTLRSVWDCINIFKTGPIILQNNVVFALVIHGLSDGVSLGAAATTESLKFVMYIAIIIHKIPAVLSLSTLMISKQKLSYYEVVTNLWAFALSTPLGYILVASFCMSVRGTSFMGPVAANLLLISGGSLLYAAFTAFSHNSVENDDPEGQEIGQESENTHRKNVMSLVAGAAIPAIVSFLIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5K7</accession>
    <name>A0A1E5R5K7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cation-transporting ATPase</fullName>
            <ecNumber evidence="13">7.2.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g3630</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.-" evidence="13"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5K7">
        <property type="interactions" value="338"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5K7"/>
    <dbReference type="InParanoid" id="A0A1E5R5K7"/>
    <dbReference type="OrthoDB" id="48943at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022890">
        <property type="term" value="F:inorganic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015662">
        <property type="term" value="F:P-type ion transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098662">
        <property type="term" value="P:inorganic cation transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000032">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000068">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047819">
        <property type="entry name" value="P5A-ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01657">
        <property type="entry name" value="P-ATPase-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF8">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12409">
        <property type="entry name" value="P5-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01229">
        <property type="entry name" value="COF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="275"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="457"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="485"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="677"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="709"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1181"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1217"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1258"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1304"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1335"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1373"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="domain" description="P5B-type ATPase N-terminal" evidence="17">
        <location>
            <begin position="258"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="16">
        <location>
            <begin position="428"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="15">
        <location>
            <begin position="523"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="144"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="158"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF12409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ81823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1422" mass="160481" checksum="E1149FBB57E61E48" modified="2017-01-18" version="1">MTVPTPTRQHRPSISSSRSSYLSETNDPWDDDHVIVPSSISTFHHNDHHLPQVVINESNHYESSSARPSIAGSSLHSERESFLHERASGSLQNAQFRFFTNQQIENAEGFTSTIENMDFLNDEDAVYEGSQTSLLSGSQLGRRRHGSFYVRPPTTYGSIHSQRSSGSSSSSSSLVYNDQHRGPLDGEGHESDGISHDEDSLLGEAYDNENFDDADLHVANLREIMDLSNYSKLSAKEHEKYFKFNPQLQVQRLYIAEEDLVVGIAGYTTLTYRVWLYYLLCIATFGVSYLFCRWFPKWKLYITSKKTALGKADFVAIETEFGDFELANVKRQWYNRALSTVLNRFNAEEEIDFDPETDFENYENRRGSNNINTTYVSHRRRSSLHDDPELPLLISFEYRFFKLFYSPVEDIFKTNTNWVDKNWKNVNKLKNGLSSNVHQDRLQVFGENKCELEIKSIFELIINEALHPFYIFQVFSIILWAYDEYMSYATCIFIISLFSIASTVYETRKSSQKLAEMSHFEYSVRCYRNGFWISIASSDLVPGDIFELSDPNLSIVPCDCVLLNGVVLVNESMLTGESVPVNKVACDKEVMSQLMFDFQNGRISSPVAKSVMFNGTNLIRTKATDGAPVVSAMCIRTGFSTTKGSLIRSMVFPSHPPVTSSSNGSKASKSKDLQTDAFKYIGYMFIIAMFGFTISAFNFKRLGLPTKLIVVRALDIITIVVPPALPATLTIGINFSLKRLKDKAMFCISPTKINIAGKLDVLCFDKTGTLTEDGLDVHGIQENYVAKGKFSKLMSNCTSIQNKLFKTCLQACHSLNYIDGEGLVGDPLDLKMFEFTHSRFKEHHDSGFTVDNKTHVFKIFEFASHLRRMSCLVREMGSSTEAQTYAVVKGAPEVMIDICXKGTIPHAYDSILKDYTHRGYRVIACAGKKVNLVSGQSFQDLSRDYVENDLDFLGFIIFENKLKTKTKSTLAVLQDAQIRTIMCTGDNILTAVSVSRECGLLPKSHRCFVPIIDENAQSANQMIVWEDVDDPDVHLDEVTLVPSSGIGEYSLAITGDVFKLLFKNQDQKLIDSKNKDAENNLDEDIANFLFSEHYIHTILLKSCIYARMSPDEKHELVEQLKQIDYCVGFCGDGANDCGALKAADIGVSLSEAEASVAAPFTSQNFQIDCVLELIKEGRSSLVTSFACFQYMSLYSAIQFITITILYGQGSNLGDNQFLYIDLFLIVPLAVTMSWSKPPSSNEKIVPKRPSANLVSPKIIVPLILHMLIILLTQIIPWMVSKNMPWYIKPVVGDQDTIDSTDNTVLFFISSFQYVFISIILSLGPPYRQPLMENTWFIMNCVVAIMFNVYXLFIRNPVDSWLAGSVMQLTYTSVPFKIGILVISVASYYLHYEIPNKFNGLFKKKQSSKLYKNLIKRCENISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R6E8</accession>
    <name>A0A1E5R6E8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein disulfide isomerase MPD2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3726</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R6E8">
        <property type="interactions" value="172"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R6E8"/>
    <dbReference type="InParanoid" id="A0A1E5R6E8"/>
    <dbReference type="OrthoDB" id="3970941at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051063">
        <property type="entry name" value="PDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE C17H9.14C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672:SF3">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184621" evidence="4">
        <location>
            <begin position="26"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="44"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="267"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ82113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="324" mass="36908" checksum="28C0ED3105D860FD" modified="2017-01-18" version="1">MVAKVGLLHCVTALLFLFVAGVSSSAEDTESTTPTGSNVHELQTLQDFYAHVNNNEHKYTFIKYYTTWCSHCKMLKPIFEKVAKAYVPFLPRQTGENSVDNTTAITLFGKPLLPTNSSTPQVSYMEVECDLWGPYHICSRLPGYPVLELITPVDDVSQKFIMQDKELRKTLEEEHYNSLGLWAQAWYNVKNFFTSLGGAAGQDLEDMQYVMRKVEYQGKRRADKICDWLDMAILHDLERNVQSKILAGDCMGDEVCAAGLQWFEGVKNNLHLEKKKLENILXNEENNTKEFANDDDALRTLKFKLHLVEKSLELSXSDPIIDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7E5</accession>
    <name>A0A1E5R7E5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3305</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R7E5"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="101" mass="11469" checksum="C0BE9FBA5A61803D" modified="2017-01-18" version="1">MSEIRKQLEARDAAQKSLVNNEVTYRNPNILPNNVGGSPQHNPLDNKASLLRPSEQXKYNNTSFEFKAKLEDQLQALEKKTQAKIKSDLRKKYGESAVSEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7L3</accession>
    <name>A0A1E5R7L3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cysteine-rich transmembrane CYSTM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R7L3"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="91" mass="9749" checksum="B9369732B007E543" modified="2017-01-18" version="1">MNNQGYYQQGPPMQQGGYYQQGPPMQQGGYYQQQQQPMYMQQQAPPKDDGCCXGCCGTCCKTLFCCCLLEMLCGDCMGPDGGPGGPGGPGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7N2</accession>
    <name>A0A1E5R7N2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Kinetochore protein SLK19</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3350</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7N2"/>
    <dbReference type="InParanoid" id="A0A1E5R7N2"/>
    <dbReference type="OrthoDB" id="5367584at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024312">
        <property type="entry name" value="TACC_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159">
        <property type="entry name" value="CENTROSOMAL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159:SF31">
        <property type="entry name" value="CENTROSOME-ASSOCIATED PROTEIN CEP250 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12709">
        <property type="entry name" value="Fungal_TACC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="114"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="198"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="426"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="736"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="133"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="217"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="770" mass="88006" checksum="C04E7C4B16B8FB2A" modified="2017-01-18" version="1">MSSPRKHKIPGSSQKNTDPLVFPSHSLLNGVSLXRKLKFPTTDHDLTENTSQIHNNDDTEDFTKNQSMPLLMKNTSGKLRFFNTLQKQAPVLVPEPSLTKITTDVRLRSIKKTAIHHSQNETNADHEEKDTAHDDDDDDDDDDDGDSDMSIDAVRRGADTAQPPLPLDVFADANHTVNSSIVESPLQNNKINFPSTSKITSRKRSFHETLSPLKNVPQLTNSNNKNQHSSGNKENVSFSNGVSFFNEDNSPGVQHQSKPLNKIPALSAPSPNNQSSPMEIKMISPERALVANEASGHKVHTRSKSGNYAQLDNDXIIESMNLSPLGSMVKRRRLSQPHSHSQHLVEALPATLKDLHQSFNRELLSYKETVEEKNSKIFHLLDSTHQLESDLAKITDKYDHLKFEKSEILKNYEIAHLENSTSVGKLKDLEEKMIESASKIEELSNENEKLSNLKIALNDEKNSHLIEISNLNSNFKTLELELDNLLNEKNQEEEEHKKLEAKLESLEKLNEQHVQEVEDLKNKLVKNSKDENESLQKKLEDLQMETANEIKTLEDLLDKETAELKNKNEQILGVQKLNKDLQEKVDEYENKITSLKDLVENLQLDLKYKEEQIQNLSTSSSTKSLDLDNQIKTLEEKNNKSRKEYEDNILSLKQEMSTLKESADKSKNERPALESKISELQKIINQTDQETNKKLQSLAEDLYAQYSSKHEQKVKILKNNFEKKYQDQVVKMRHQTTAAQEELAMVKKQLEHERKEKENLLQMWEQQSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7X2</accession>
    <name>A0A1E5R7X2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mitochondrial 2-oxodicarboxylate carrier 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3390</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7X2"/>
    <dbReference type="InParanoid" id="A0A1E5R7X2"/>
    <dbReference type="OrthoDB" id="434783at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051752">
        <property type="entry name" value="Mito_2-oxodicarb_carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356:SF1">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="8"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="106"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="201"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ83009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="293" mass="31707" checksum="A7ECDF0728453566" modified="2017-01-18" version="1">MSIDNRPLPGYYSIISGGTAGVTELLIMYPLDTVKTRMQLKGGATAAGANNGVFSILSNIVKTEGPLRLYKGLLSPIFLEMPKRAIKFSATDEFRKIYGEKLNWGPSPKLQVISGISAGVLESFLVTPFELVKIKLQDPNSAFKGPKDVISSILKSKGIIGMATGLEATMWRQSVWNAGYFGSISHIKSLLPQVSPEEKGKTLFNNFIAGTIGGTIGSTMNIPFDVVKSRIQLSGQGGSALSEIFKIAQKEGVKTLFSGWTPKILRLGPGGGVLLVVFDQMMGMFRDIHYKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R838</accession>
    <name>A0A1E5R838_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ATP-dependent permease PDR12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g3288</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R838">
        <property type="interactions" value="215"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R838"/>
    <dbReference type="InParanoid" id="A0A1E5R838"/>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000054">
        <property type="entry name" value="ABC multidrug transporter atrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="535"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="567"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="621"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="679"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1205"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1234"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1277"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1316"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1349"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1471"/>
            <end position="1489"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="171"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="864"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1510"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1512"/>
            <end position="1530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ83072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1536" mass="174244" checksum="15223030B372D4B3" modified="2017-01-18" version="1">MSSTGSSYKEEKVNNTSEEDNAPKALNQEPKLDHDDVSSYAGSVQSYENEQEQLHPEGERDIESQNIESQLSRHVTHILSETNGAERIESLARVLSTKTKKQLEHFEIKEGIDFDLQQLLDYLRSHQLDQGIVPGDAGVAFKDLTAVGIDASAAVAPSVAEMVRGWFHHPLRAFSRKKTDMVLRNIIQNFTSVIESGEMMLVIARPGAGASTLLKCIGGETSELVEVTGEFSYDGLDQHEMMSRFKGYVIYCPELDFHFPRITVKETIDFALRCKTPRVRIDGMSRKEYVDSMRDLWCTVFGLRHTYATNVGNDQVRGVSGGERKRVSLVEAIAMNGSIMCWDNATRGLDASTALEFAQAIRTATNMTNTAAIVCIYQAGQNIYELFDKVTVIYNGKQIYMGPAEEGVEYFTNMGWVKPNRMTSPEFLTSVTLDYDNRVLDVKPGFEDSIPKNSQEFLDYWVNSPQYAKNLGQYDEYVANHNAEETRQRLAESIEQRKQAREPVNSLYVVNYWHQVWYCMIRGVQRVKGDTTYTIVYLSSFLTKGFFVGSMFWRIDPIDQSTTEGAYSRGGLIFYVLLFCSLTSLAEISNSFANRGIIVKHKTYSMYHTSAEALQEIFTEMPVKFVAVLTLSLVSYWLPALKWQAGSFFQYLLYMFTVQQCTSFIFKLCATLTKDGGTAHAVGGLWVLMLSLAASFFLPIGQMHHWMRWFHYINPLTYAFESVMATEFHGRQMLCSKLIPSGTNYENVSIENQVCQASGAVKGQRYVSGDTYLKKAYHFEYKHAWRDWGINVVWTFGYIIMNVLMSEYLKPLEGGGDLLLYKRGFMPSFGDNADAKVASREEMMEALNGPNVDLDEVIASKDVFTWNNLTYTIPYDGSTRVLLNSVFGYVKPGKMTALMGESGAGKTTLLNTLAQRIRMGTITGDMLVNNKPLPASFKRSCGYVAQADNHMAELTVRESLKFAAELRQPASVPLDEKMDYVEKIIRLLGMQNYAEATXGKIGRGLNVEQRKKLSIGVELVAKPSLLLFLDEPTSGLDSQSAWSIVQFMRALADSGQSILCTIHQPSATLFEQFDRLLLLKKGGFMVYFGDIGENSNALTGYFERQSGIKCGAEENPAEYMLNRIGAGATANASADWHELWENSPECAAGRQELAELHEKLNARPVTEDKELSSRFAVPLLTQFKAVLWRTNIQFWRSPVYIRAKFLECILCALFVGLSYVGTGVDYGIQGAQSSFASTFMMLLIALAMVNQMHVFAYDSRELYEVREADSNTFHWSALLICHTWIEIMWSSICVFICFICYYWPAQFSGRASHAGYWFLIYVIMFPIYFISYGSWIIYMAPDVPSASMINSNLFAMLLLFCGILQPFHKMPGFWTFMYKVSPFTYVVQSLVTSVVHDKKVICNPHEFAILDPPQGQTCEQFLAFYRDEHKGYIDNPDATDNCRYCPYTVSDQIVEQYGIRWGQRWRNFGLLWAYIVFNFVAMCVCYYIMRVKVWSVKGILDIKKWYHGPRKERHEPDTNIFKEKPHDKSNVAKAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R868</accession>
    <name>A0A1E5R868_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ATP-dependent permease PDR12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g3290</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R868">
        <property type="interactions" value="215"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R868"/>
    <dbReference type="InParanoid" id="A0A1E5R868"/>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000054">
        <property type="entry name" value="ABC multidrug transporter atrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="554"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="595"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="639"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="704"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="810"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1227"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1256"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1299"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1338"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1373"/>
            <end position="1390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1493"/>
            <end position="1511"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="184"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="886"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1532"/>
            <end position="1555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ83100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1555" mass="175919" checksum="EC8A466F50195174" modified="2017-01-18" version="1">MSDLYKESEKDSHQSSSSFENNGGNWKIAEKQNVHATDTNTTTTNNNLDNSKEDNSDEFSVADSVHSLNENNVDDITNFDRDVESQVTETQLSRHVSSMLSENDGADRIEALARVLSTKTKKEMNNFEIREGADFDLQQLLDYLRSHQYEQGIVSGDAGVAFKNLTAVGIDASAAVAPSVGELLRSILITPWKKLLMRKSLKKPLRNIIENFTGVVESGEMMLIISRPGAGASTFLKCIAGETSELVDLNGEFSYDGLDQHEMMSRFKGYVIYCPELDFHFPLLTVKETIDFALKCKTPRVRVDGIKRSQYVNSMRDLWCTVFGLRHTYATNVGSDMVRGVSGGERKRVSLVEAIAMGGSIMCWDNATRGLDASTALEFAQAIRVATNMTYSSAIVCIYQAGQNIYELFDKVTVLYNGKQIYIGPATEAVTYFENMGWIKPNRMTSPEFLTSVTLDYDNRVLGVKPGFEEKIPKNCHDFVEYWVNSPQYAKNMGHYDDYIAKHNPEETRQRLAVSIEQKKQHREPLNSIYIVNYWHQVWYCMVRGVQRIRNDMAYTKIFISVYLIQGLIVGSMFWRIDPIDQSTTEGAYSRNGLIFYVVLFCAVTSLAEIANAFVARPIVVKHKAYSMYHTSAEALQEIFTDIPVKLVSIILLCLVTYWIAALKWTAGAFFQFVLYMFTVQQCCSFIFKLAANLTKDGVTAHTVGGLFFLTLITTSGFFLPIGQMHHWIRWYHYLNPLTYAYESAVSTEFHGRQMLCSNPIPTGPGYENVSVTNQGCVSLGAVKGXRYVSGDDYIKQGYHFEYKHAWRNWGVNVVWTVGYIIMNVIMSEYLKPLEGGGDLLLYKRGFMPSFGDNADAKVASREEMMEALNGPNVDLDEVIASKDVFTWNNLTYTIPYDGSTRVLLNSVFGYVKPGKMTALMGESGAGKTTLLNTLAQRIRMGTITGDMLVNNKPLPASFKRSCGYVAQADNHMAELTVRESLKFAAELRQPASVPLDEKMDYVEKIIRLLGMQNYAEATVGKIGRGLNVEQRKKLSIGVELVAKPSLLLFLDEPTSGLDSQSAWSIVQFMRALADSGQSILCTIHQPSATLFEQFDRLLLLKKGGFMVYFGDIGENSNALTGYFERQSGIKCGAEENPAEYMLNRIGAGATANASADWHELWENSPECAAAKQELDKLHERLNSRPATDDKELSSKFAASLVTQFKCVLWRTNIQFWRSPIYLRAKFSECTMCALFVGLNYIAAGKDHGIQGAQQSFASTFVMMLISLAMINQMHVFAYDSRELYEVREADSNTFHWSALLICHTWIETLWSAACIFLCFICYYWPGQFNGKASHAGYWFLIYVVMFPLYYVSYGSWIIYFSPDVPSASMINSLFYSMLLMFCGILQPYHKMPGFWTFMYKVSPLTYVVQSFVAPLVHGKKVICKANEFVMLDPPQGETCSNFLNAFSQKNPGYLDNPDATENCRYCPYSTQDEIVAQYGVKWDYRWRNFGLLWAYIVFNYVAMCVCYYYARVKVWNFKGILDLKKRYKGPRKERHEPDKNIFKAQPGDKNIVPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R8Z5</accession>
    <name>A0A1E5R8Z5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Regulator of V-ATPase in vacuolar membrane protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3169</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R8Z5">
        <property type="interactions" value="74"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R8Z5"/>
    <dbReference type="InParanoid" id="A0A1E5R8Z5"/>
    <dbReference type="OrthoDB" id="66510at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043291">
        <property type="term" value="C:RAVE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028241">
        <property type="entry name" value="RAVE2/Rogdi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13618">
        <property type="entry name" value="LEUCINE ZIPPER CONTAINING TRANSCRIPTION FACTOR LZF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13618:SF1">
        <property type="entry name" value="PROTEIN ROGDI HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10259">
        <property type="entry name" value="Rogdi_lz"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ83003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="397" mass="45261" checksum="18C1639267C491D8" modified="2017-01-18" version="1">MTTELYPNDYFSNKGKKEPDHELKWLVDEIIKPQLPDLLDTLEKCAQMLDSEESFKLPITSKDDNILEYIQHKGSKTLSSNAGXDTTHSSCGGLLKGTITRKKGAIVDYQLLVQFTKFRKNQPQMLHMCRHSNISSNDPNFSNSEAKIKEKNNEXDPTDAKLESQLLDQEHAEIELHQLIATQKYTNIAINIIQNLEKTTDVHEFKKLFQELNQLFVLLINTITKPMDFLQFPLFTKPLLPQLESQHHLLVPEVVIISNEIVLEIRCLQKFDKKPWSNINKEGKSTCDLIKMELYDHKKKLTDILHSHHINIVEPSLFHSIMTYSSSGTSGGGQFANVTLTEAQDILSRSVTYQNQLFLEMGKVAVSTSDPYLITVCAKLAALQKTIYNAHLNLQFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA88</accession>
    <name>A0A1E5RA88_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Exportin-T</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Exportin(tRNA)</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">tRNA exportin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g2797</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">tRNA nucleus export receptor which facilitates tRNA translocation across the nuclear pore complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
        <text evidence="9">Shuttles between the nucleus and the cytoplasm.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the exportin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA88">
        <property type="interactions" value="1081"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA88"/>
    <dbReference type="InParanoid" id="A0A1E5RA88"/>
    <dbReference type="OrthoDB" id="26399at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071528">
        <property type="term" value="P:tRNA re-export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045546">
        <property type="entry name" value="Exportin-T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040017">
        <property type="entry name" value="XPOT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15952:SF11">
        <property type="entry name" value="EXPORTIN-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15952">
        <property type="entry name" value="EXPORTIN-T/LOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19282">
        <property type="entry name" value="Exportin-T"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <keyword evidence="6 9" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Exportin-1/Importin-beta-like" evidence="11">
        <location>
            <begin position="150"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Exportin-T C-terminal" evidence="12">
        <location>
            <begin position="417"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="domain" description="Exportin-T C-terminal" evidence="12">
        <location>
            <begin position="973"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF19282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ83818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1174" mass="132872" checksum="14A7B8EACF471DA8" modified="2017-01-18" version="1">MSSEQMIQQLIQAIDLANNPASQADLNSQALQFLNNTKTANNAPEIFIELIKYASRSSQSSQLSSSSSSSSTLVSATPTSPNNNIYKFVGFQALAELIPNANINQLQFIKSYVIEYVSAALAQANISSNSNNNNNNTNNTTNNTNLTSDPEYVRNKVCEIFSILFYFMYGQCNNNLWNTFFQDLMSIFKLEPIITCPSSIINNRVCQLGLDYFLRITLQINTEIADQTFVVNKQIQQKNNNLKDFMRINDITNLTIVWLNVLMNLESVETVINLALTNVGSFVSWIDINLIVQPQFINSIFKFLDTNTIACCTCLSEIIAKKMKPADKLQLLQLLNLTDKITQLFNTQDLEIIENLAKLVNAIGIEYTIICENASVNESSNNNNANNNTKNGNKNLNNLTNGNIDPNNSEYVQMADTNILENVCPLILQFLSNEEFFEVNLQVLPFISSYLSFLKKNFALGGKPGSAVNLQSRKLTIDQAHLTFCKNLLIVVHNKSLINDNFDIDSIEEINEFDENFRSKIKNFQDSVAVINPEIYLNHMVEIVSQNGMLFNRDNTTIEEWRIEELFIYQLHNYAESIRNNLFGIQKNAVIESKPFLLMCEFMKNKFLKNESILKYVANPWIQISYFELVVRHHNFLLVDQTASQENILYVLNIFASEFGMFSSLEKVRFRCWFLFSRFIKLVKPSLDFSSLISFFTKISPLLIIDVPPTQQAAENTLVNEISNQDITFENQLYLFEGAGILVSSSKSLDFSILDGVLNPLFRSLELCIGAPLKTCSTVLEIHHIMMAIGTLARGIHSGLVPENLLSNQPTNEKLLANQSLIEVFSNIAEVVLVTFQYFHSFELVRDATRFVFARLISILNTNIISFINRLLATFYETEMKVVEMNDFLGFVGQIVHTFQNNEDCYQLLNNMISPLVKKVFKLIEDTKLESSSSSTTAAVTSASPSPEXVGXSGNTTVVVNANGAKDKNVVVTDAYRDIVEIKRGLYNFMQSLITNHVTSLLLTETNKNXLSVVLNDLLLCTPQDLKETTVMKIKMNVLINLTKYLGTGSCVDPDDAHSNDLIKLEGLNEFFIAKIVPLVFEIPFNPEYAFNIKDGSYRVVASDLSRLLKVCYLSNFQSDGTPNPLLTYMNDFYFPQIQFPSSFTMEFIQALEQLNEKKFDQYYVGFINKIKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAA1</accession>
    <name>A0A1E5RAA1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome c oxidase subunit 4, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cytochrome c oxidase polypeptide IV</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g2823</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome c oxidase subunit 5B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAA1"/>
    <dbReference type="FunCoup" id="A0A1E5RAA1">
        <property type="interactions" value="452"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAA1"/>
    <dbReference type="InParanoid" id="A0A1E5RAA1"/>
    <dbReference type="OrthoDB" id="10249250at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00924">
        <property type="entry name" value="Cyt_c_Oxidase_Vb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.11.10:FF:000003">
        <property type="entry name" value="Cytochrome c oxidase subunit IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.11.10">
        <property type="entry name" value="Cytochrome c oxidase, subunit Vb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002124">
        <property type="entry name" value="Cyt_c_oxidase_su5b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036972">
        <property type="entry name" value="Cyt_c_oxidase_su5b_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10122:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 5B, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10122">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 5B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01215">
        <property type="entry name" value="COX5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57802">
        <property type="entry name" value="Rubredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51359">
        <property type="entry name" value="COX5B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602124-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ83835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="156" mass="17160" checksum="08EE6E19883473C3" modified="2017-01-18" version="1">MLSIRSHIARSTRQFSVLRTVLNEHHQQQPVKFHTAKTLSEVDGPQSLIGEGAAPGTVATDVEQATGLARLELLGKLEGIEVFDTKPLDSSRIGTMANPIMVDSYDDYRYVGCTGSPAGSHPTMWLKPNVNQISRCWDCGSVYKLNRLGSKTDNHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAD3</accession>
    <name>A0A1E5RAD3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2767</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAD3">
        <property type="interactions" value="2054"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAD3"/>
    <dbReference type="InParanoid" id="A0A1E5RAD3"/>
    <dbReference type="OrthoDB" id="295274at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14687">
        <property type="entry name" value="bZIP_ATF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="2">
        <location>
            <begin position="528"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="335"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="500"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="53"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="237"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="353"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ83854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="599" mass="69053" checksum="A58FA93A52834B23" modified="2017-01-18" version="1">MDDITTEDILYNNLSSSNKSSSSNPSEDGKSSQVNQPMENLNISQTSQENLHQQQQQMKKHQQQMLVQKQLQAKKQQQQQMLQQQLQTQKEREFHQQNLERQEGTPNNVAQQPLSLFKNPFEELPAQTMERGSFDSYGKNASGHNSRGSASSKQDFHQQKMSPQGQVQYQQQQPPPVLHPTQQHXSRQHMYHSKDKTIQQQLNPFYQPYGIDVSHFPLTNPPIFQNLMFDDSSFPTFSTDTSTTPSPSTTKLSNGEQANNNTTNEHHTHLGQADVSKSKAPLKTNTELFKEQQMQRQSSRNRYPFQRRISISNGQIGQLNENAIMVDEVYYTQPPPMPNTYFQNHPQQHHLQQHQQQQHPQQHPQQQQQQQQQQQQQQQQQQQQYHGDKNSQKQEQHLFGSDLDGQMHGSPQLENKTVAIKRENSASTRGFGFLDYNNNPQKVQSRSQMNTGMGIDFGEYGDNKLVLNSDILNGSDAAAAAAMAGINAPSMEMLSSNESYLQESNSKRKSGARRILPPSTEFVPGTDEWKKSRLLERNRIAATRCRHRKKLQQEQFMKEYLDLKYNNIELNNKVVLLERELIELKQRLMDIVSINNVQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAF3</accession>
    <name>A0A1E5RAF3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Alpha-factor-transporting ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2788</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAF3">
        <property type="interactions" value="777"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAF3"/>
    <dbReference type="InParanoid" id="A0A1E5RAF3"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000604">
        <property type="entry name" value="ABC transporter B family member 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF15">
        <property type="entry name" value="ALPHA-FACTOR-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="256"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="725"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="769"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="845"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="874"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="19"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="357"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="728"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="1065"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="11">
        <location>
            <position position="766"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="11">
        <location>
            <position position="804"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ83851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1311" mass="147291" checksum="A4C56B1F1D022BA0" modified="2017-01-18" version="1">MLFEKNIYKFVKIEKDWKILLLLVALTLVEGVVPCVVSVLVGRTFGVLSNAAQGKYLTKHAMLHDLTIRAMSIMCCAAGSFPICWININNWMQFGERQGLRIRNSIMRKYLNLPMQWFNVQENLMATFTQTNRCIEELRSSAAEASGITLQNLVTCCGLLGVSFYYSWSLTLIFLCSSPLIIASSIVCSLEYEKYLNLENNESKLASNILLWCMENIRIVKGFQTELLESANFKQKAENCNRFFVKAXMYIATNESIIRILSLIMFVQGFWFGNHQIKRGKLNTTQVMVCFSCCVTLAATLMSTLQQILLMQRGKIALEYIDNLYDPSNDLSNSFSNDKIDQKLKPLNNPSFSEYEIELSNVSFSYPSRPKDTALNNVSMSFGKEKTTFIIGKSGSGKSTVASILLKLYENYQGDXKVNGLNLKNINDNWIYSNVLLIEQNCTLFDDSLRNNILLGSNANNNINASEVEELLIGACTSALLNDFIASLPNGLNTWIGKSSQNGIQLSGGQQQKVAIARAIMSRKAVWIMDEALSALDSGQKDVIMKNLRXIRKKQTTIVLTHDLTHIAEDDYLYYMDTGKIKEHGIQKNLLSNQTSNFKSMYELQHIRKIQTSHQNSLLNKSDNKTLQEERTTRLETTRETNPVLEQLDCNTKVWTKTQQKFLESPSFEKTDPQVEVDSEKDTLSVCTKICDLESSLTNGDDTRIPLMPLKAVTQNMLSTIQKKALLFIGLLCAVLAGASNPIFSWTFAKLLNQSVXNKDNTAKPDSYLIKWSIVVISISIADGLLTLGKSFLLKYVAEYWIYDLRLLGFNKVVNQDLTWFQEALNKPSEIAALMLNDLRDLRSLVSEFTSAVTTLLLVSLIGLIWAIVTGWKLSLVCLSLIPLYAIFTGIYGALLQKAETSYKSKIADLENKQYEVVSNIKSIRLLQLESHFLLQMDNLIHNLQKAIAFRAWATGLGVAIFKTITYSVQSILLYYGLKLVCDGEYSAERMFETFTLLLFTVTTCSTLFNNIPDMARGQRAASYVFRVIQSSIQSSIQSTAFQDSQIKNMIXKQNTXESSSHTMIKFTNFCFSYPSMPNVSVFKNLTFEVEKNEIVGIVGKSGSGKSTIASLLLMLYPIYQVKALEIKDCDINDVGVSAIRKIISVVEQKPSFFRSSIKENLLYGCNTHTNSETVLWELLKKLELESFVNALPDKLDTVIDSSLISGGQAQRLSLIRSLMKKAEILVFDECTSALDATNADIIENFVTSRTESLLNKTTRVIITHSETMMKKCDRIIVLGGTDGVVVETGTYEELAAKKSSYFNMVLMEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAZ6</accession>
    <name>A0A1E5RAZ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein SKT5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2990</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAZ6">
        <property type="interactions" value="94"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAZ6"/>
    <dbReference type="InParanoid" id="A0A1E5RAZ6"/>
    <dbReference type="OrthoDB" id="272077at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051726">
        <property type="entry name" value="Chitin_Synth_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430:SF1">
        <property type="entry name" value="CHITIN SYNTHASE REGULATOR SKT5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430">
        <property type="entry name" value="PROTEIN SKT5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="105"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="652"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="108"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="652"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="3">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="3">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ83743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="695" mass="76944" checksum="DB4C9EBC35E40B68" modified="2017-01-18" version="1">MTRQDTLFEPPSEWLHLARVDDKDEELAKEGLIPDIESGFMLHTPHGKEEKTVKMEQEANSTKALLTTESSIEKTGIGKKDLMKKFSSFHILASDSSSSESLYIPVASEQSRTPSLDSQGQEKKNSKSDLDXQLSLSPPNEDFKLSDXVSSKDNALFVVPTKLISGDIQESSNTGLRDNDEDXPAEMQPSTYTSTEVANQTFRANEIAGTEELDAILLPKLKAMDDFKKTIQKTKDSAVFLDYSKYMWETGLELGLHESQANKNELLXLKKQFLKESVHYLKKLSVKGYSTAQYLLGKAYSSTELGKPNHKEAFILFQTAAKHGHVEAAFETASCYEKGIGTLRDSRKAVEFLKYSATRHHTMSMLKLGTYSFYGKMGLSKDLNTKQNGIKWLTRAVACSDKSTCSAPYELGKIYEKGFLDIIIPDQKYALNLFIQASALGHSKSNTKLGQIYERGNSCVQPNSALSIHYYTLAATDTLYPDPQAQLSLCAWYLVGAKGVLEADQQQAFLWALRSAXSGHPKAQYAVGNFYEKGIGCRIDFNEAFIWYEKAAKNNDSKAISKIKKMTGFDTNDIAASGAKKKDQKNPLKSSNSQSSTLLSSFISNLDDSFGESFFQKKNDKNTHVNIQNTKYNADDVVLPIALPTTGSSISSKLNTTDAASSKPSLTTKSNKHXKNSILTAEQTTEDSKAKCLVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB26</accession>
    <name>A0A1E5RB26_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutamine synthetase</fullName>
            <ecNumber evidence="3">6.3.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3061</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glutamine synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RB26"/>
    <dbReference type="STRING" id="56408.A0A1E5RB26"/>
    <dbReference type="InParanoid" id="A0A1E5RB26"/>
    <dbReference type="OrthoDB" id="1936100at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000011">
        <property type="entry name" value="Glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050292">
        <property type="entry name" value="Glutamine_Synthetase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852">
        <property type="entry name" value="GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852:SF57">
        <property type="entry name" value="GLUTAMINE SYNTHETASE 2 CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS catalytic" evidence="9">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ84098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="107" mass="11947" checksum="132B3A93D5AEC552" modified="2017-01-18" version="1">MKLIEEAIEKLGKRHLDHIKLYGTDNDQRLTGKHETASMTKFSSGVANRGCSIRIPRSVGLEGYGYFEDRRPASNIDPYLVTGIVTETICGPVNGVDMVEEFEKESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB37</accession>
    <name>A0A1E5RB37_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Importin subunit beta-4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3016</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB37">
        <property type="interactions" value="1427"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB37"/>
    <dbReference type="InParanoid" id="A0A1E5RB37"/>
    <dbReference type="OrthoDB" id="7862313at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057672">
        <property type="entry name" value="TPR_IPO4/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25780">
        <property type="entry name" value="TPR_IPO5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="9">
        <location>
            <begin position="26"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="8">
        <location>
            <begin position="403"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ83783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1118" mass="123825" checksum="3155763A96441D7B" modified="2017-01-18" version="1">MDQYLAELEQTLQAIVVPNSANLKDATKLLQTRFYADAKCVPALIHLLVNSNDSSIQQLAGVEARKQITKYWEDLDASTTNDIKASLLSKTFTCENKNVRHTMARVIGEIGSLELDEKKWPELMPYLFQTASSTDNAQIRETAIFVIFTVLEQFNATLGLYVDELLDLFSMTIVESPQSTLETRSLSAQAIDLVSQLIEEQSTKNPXQIHKFAALVPAIVNVLEAVIQAGDSVNAKLIFNCFQDFLLLDSQIIGNAVLDLINLGIQISSNAXIDEDCRIFAMRFVVSALSYKKNKITQAKLGSEITLTALKIASEEIDEEEELTNEDEAGENEEDTPSLLAVRMLAYAATELPPSQVANVIVNNLAPMLQSSNKYAKRAILLAISVAVNGSPEYILTQLESKIVPATLQGLKDPEPIVQLASLKALHSLSVELQDDVAKYHEQFLPAIIEIIDSAKSVVIYRYATMVLDGLLEFIAYEAIVNYLEPLMNKLFHMLDTNTSSKLRAVIVSAIGSAAFAAGSAFVPYFKPSVQYLEKFIQNSSDINGMSEDDIELRALTFENISTMGRAVRSEVFAEFAEPLVSSAYEAIKTDSARLRESGYAFIANMAKVYGKDFAPFLANIMPEIFKTLQLDEYEFNLDEEDEEFQGLTEEDLMSKFNVNTGVTYEKEVAAAALVELALATKESFLPYVEETITILKQQVEESYGLKETVIVTYWAVVKSVILTSQPALENKFVKGLPTAAYVDPSTLAVIESAREVSLENLVDEAEVSVVTTIFETISEMISKFGSIIVATSASATESLDTLCLQVLEVLKGEHSCQVIDFEEDAPKDEEEEMESNETETILTTTALEILVSLSTALTGAEFGNIFNTFKPVIFSLFNSKSKGKRVAVVGAVSEIAFNLGESNTCIQEMLEQLIIKLTSDKNLEVRGNAAYGVGALIRNASFDVTSVYQPILKALYEILSAADQKSQDDDDETTRETLDRSFANCNGCVGRMILKHDNLIPLEHTLPALLNHLPLSTGYEEYDPLFELIIKLYGSENALIMNETPKIVSVFSDVFKKETERVRLERESTLGREENLDKMKQFQSEEMKQKVVELLKFLNTKFNGIVSNDPILAQVIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBP9</accession>
    <name>A0A1E5RBP9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bacterial surface antigen (D15) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2695</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBP9"/>
    <dbReference type="STRING" id="56408.A0A1E5RBP9"/>
    <dbReference type="InParanoid" id="A0A1E5RBP9"/>
    <dbReference type="OrthoDB" id="1724197at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019867">
        <property type="term" value="C:outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.50">
        <property type="entry name" value="membrane protein fhac: a member of the omp85/tpsb transporter family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bacterial surface antigen (D15)" evidence="3">
        <location>
            <begin position="149"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ84319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="474" mass="52627" checksum="26A72984E0549923" modified="2017-01-18" version="1">MSELAWVKPDVQNTELRDQQSLPLRINKVTVNQNPVDDVRVTTNTATNFLLSEKFYQSLLQPILEQPIQNAHNTIEQLKTIQHKIYQTGLFEHVEFKVDVEKMGQSSSISGPPNGLLPTPLPVSTTINLFPKVLNENFLTTALSTANLNNNEIVLNSIKYNTLGKAEKLLYTLGLKYDLESNSFKSSLAKAQFLFPLPKNPSVNGVFALNYVNSDRLFNDDKHFNFSLGLLKNWKSCELFGGLAFTRRSVGDKSKTSLIGSYIKDKRSFVGMLPVQGSYIEVNNDLVFAQARNGTIENDNANYNNFNMKLDLYKSWFQNALTSSLETSFGAIASTAAAGNSLIVDKEDQLDFPNLKGFQYQSKSIKDLGALNCKAALSCKLPFQNKLSPLRFQMFTSFNQMFNPAASSVNNKNSMGTTSSGLLQNDKLYSSYGWSLIYKTEMADLDLSYTIPVSNSGSSIAAPGFSIGCALSFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBV2</accession>
    <name>A0A1E5RBV2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">RNA-binding protein PIN4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2973</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Regulates global gene expression after oxidative stress. Interacts and stabilizes mRNAs and may regulate their transition between different cytoplasmic components after oxidative stress.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with csx1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBV2">
        <property type="interactions" value="292"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBV2"/>
    <dbReference type="InParanoid" id="A0A1E5RBV2"/>
    <dbReference type="OrthoDB" id="434258at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12253">
        <property type="entry name" value="RRM_PIN4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000183">
        <property type="entry name" value="R3H domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034186">
        <property type="entry name" value="PIN4-like_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16148:SF14">
        <property type="entry name" value="MYND-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16148">
        <property type="entry name" value="NF-KAPPA-B-REPRESSING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="75"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="477"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="536"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="674"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="768"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="863"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="341"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="10"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="46"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="477"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="549"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="674"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="693"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="768"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="899"/>
            <end position="908"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00055199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00062407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ84378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="908" mass="98569" checksum="2CF8B389A40A9806" modified="2017-01-18" version="1">MTEKDEDPSKSTPIARSTDINGETDQANSNDNILTNDNGDVLANTEGNVGDVNSNSVNESLSTSRSGNPDDVIPTAIVIKNIPFAIKKEQLLDYISNMNLPLPYAFNYHFDNGVFRGLAFANFTTVSETTEVVETLNGKELGGRKLRVEYKKLLPQHERERIEREKREKRGQLEEQHRNISNLSLSSITSATATSQMNLALNNNTNLNNNGSNAASQTTMNFITGQMNASNINNVSSINLSNNNNNPGFQNLPPLTAQNTSTSMTSSTMANPTSSTALFGGSTSALHPYGLNSSAINNYNSMAQNANANNIGVANXKNNIVQNKNSNNSVYGSYHGSSVHLQSYAQQQQQQQQQQQQQQQQQQQQQQKPQMIQRFYAPVPSTSNLPFPPQQIDFNDPDVLEIYSQLLLFKDRDSQYVELCYQQSGLSATHKRYINVLCMFLGLYEVYDPAFLIIRRSSNGNTTNKTGSTLELNSAAQNNVNNGNMGYNQNSQGTMQNANSASATSIYNQSFTMMQPSLQPMSTGGSINKSHSYTSLLQAHHHASETAKLESSNNASISGNPQDNTSPPQKYQQNVSSAMRPQSSNLLQNGRIPSSASQLGANSNVQNLNINAQTSSTSAQIQTPNVITSSQGFNSSTLLSNPLLRNQGVSPPILNAIPATPSLVSRTPFQNAAAAAASSSTPTTPNVGSSAANNNNNNNNNNNNNNNNNNNNNVTSTMVQGNLQIPSQLMPQSTSGSIHSAYSAHSGFHDAFGGMNPGTSLSQLAGQSYSQMSHYPHQQQQXQSKHQAYHHLQQSQQSQQQQQAQSPYSSMGMLNLLQQQKSPQGNSSQPVSALNGVDPNGFYSATGMSLSSSVQLQLNMNEQNQQKDKANGEPLSSAFGNQEEHTGAEDDNEHLDFDISQNLTGLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RC23</accession>
    <name>A0A1E5RC23_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2639</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RC23"/>
    <dbReference type="FunCoup" id="A0A1E5RC23">
        <property type="interactions" value="1083"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RC23"/>
    <dbReference type="InParanoid" id="A0A1E5RC23"/>
    <dbReference type="OrthoDB" id="1162399at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060213">
        <property type="term" value="P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045948">
        <property type="term" value="P:positive regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04329">
        <property type="entry name" value="RNAP_II_Rpb7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04462">
        <property type="entry name" value="S1_RNAPII_Rpb7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000043">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.120:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005576">
        <property type="entry name" value="Rpb7-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF4">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03876">
        <property type="entry name" value="SHS2_Rpb7-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="N-terminal, heterodimerisation domain of RBP7 (RpoE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase Rpb7-like N-terminal" evidence="8">
        <location>
            <begin position="3"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="7">
        <location>
            <begin position="59"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ84123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="150" mass="16611" checksum="4C1551D1D149DD16" modified="2017-01-18" version="1">MKQFLRTKLLQDVEGTCTGKFGYILCVLDCNNIEIERGRILPNDGSAEFNVKYRAVVWKPFKGEVVDGIVSTCTDLGFEVDVGPLNVFVSKYLMPEDLFFNSASNPPSYQSSEQIISKGSRIRLKIVGTKSGVNSIYAIGSIKEDYLGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDR8</accession>
    <name>A0A1E5RDR8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Mitochondrial DNA polymerase catalytic subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2226</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDR8">
        <property type="interactions" value="778"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDR8"/>
    <dbReference type="InParanoid" id="A0A1E5RDR8"/>
    <dbReference type="OrthoDB" id="5588663at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005760">
        <property type="term" value="C:gamma DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006264">
        <property type="term" value="P:mitochondrial DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.390">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019760">
        <property type="entry name" value="DNA-dir_DNA_pol_A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002297">
        <property type="entry name" value="DNA-dir_DNA_pol_A_mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001098">
        <property type="entry name" value="DNA-dir_DNA_pol_A_palm_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041336">
        <property type="entry name" value="DNApol_Exo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10267">
        <property type="entry name" value="DNA POLYMERASE SUBUNIT GAMMA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10267:SF0">
        <property type="entry name" value="DNA POLYMERASE SUBUNIT GAMMA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00476">
        <property type="entry name" value="DNA_pol_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18136">
        <property type="entry name" value="DNApol_Exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00867">
        <property type="entry name" value="DNAPOLG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00482">
        <property type="entry name" value="POLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00447">
        <property type="entry name" value="DNA_POLYMERASE_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family A palm" evidence="7">
        <location>
            <begin position="756"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="328"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1173"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1247"/>
            <end position="1266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1276"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="328"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1278"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="8">
        <location>
            <position position="375"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="8">
        <location>
            <position position="1178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ85039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1297" mass="147615" checksum="D15EF44A60D82544" modified="2017-01-18" version="1">MRVSKGRHIELSKAIVRSGCRGQSFMAFSCTLTNAGKRDFSTSKLVLLEEGKLAHTIDPKVKQNAFGIQYLAPSLKSQVFINAEKSKGKKIQKKYSPANAPKFMEDESQFLSKLAVKGLKNHNLYGKKTSINSPIEMKLPKLQGFSLDEHFQKLGKHMSEPYLSMAKSKFTKTLPKPEKWEMKSGSWIRYAPGSQPEKVDFPLEDLYVFDVETLPKISPYPILATALSDKAWYSWCSPFLTNYVENNKTGYEHLIPLDTQNPQRKQLIIGHNVSYDRARVLEEYNFKQSNAFFLDTLSLHLGTAGMCSRQRNLWQKATKVLRNNGKLEKELASKESSETRSEEESDEFEDEMVGFLPQSGSLSIXGVDSEDILKDDPWVKVTTMNSLKECALFYCGINLEKDKRDVFVTSTDKRDVINDFQNLCNYCAEDVIATSLVFDKVFHNFXQKTPHPVSFGALKMISQCFLPTNANKWESYIKNAEELYQSSKIDIENKVIDIVQDLVKLKDNPEIYLNDPWLKQLDWTIKPLKLTKQGVPYKRQKLPGFPEWYKNLFPTASSEKPVITIKSREIPLLFKLSWENKPVVWCKEDGWCFDVFEQKEIEIMREKSYQEVKSSKDSKSSGVRFKVPHPNGPENRTTTLLSKPFVHFFEKGVLTAVSPVAQKALQINASGSYWMSARQRIMNQWVVSKEKFPTEFNFNSPMSDGQHGEPKSLPLESTVGDDGMIIPKIIPMGTVTRRAVENCWLTASNAKKNRIGSELKSLIKAPDGYSFVGADVDSEELWIASLVGDSVFKTHGATPVGWMCLEGSKNEGTDLHTKTAQILGCTRNEAKIFNYGRIYGAGLKFAAQLLKQFVPSMSDKETVDVATNLYKETKGKKINIPTVGTIWYGGSESVLFNRLEEISDQDIPQTPILGASITDSLLKGNLKKRSFLTSRINWVIQSSGVDYLHLLCCSMNYLIERYQIDARMVISIHDEIRFLVKNEDKHRAAMALQISNLWTRAMFCEQLGMNDLPQNCAFFSAVDIDFVLRKEVDMDCITPSNQVSIEHGETLDIYKLMSMADSALGKPLTHVDFSKYEYEPREKVVETLEKRLDPLLKKYLVATEVQNRKTVADALIKEYKDNXLQKSFVDEYNKLLDETIDFNDPLRKKKIGQPEKESQFSFKALKKSLEYKPQXTQDSXLSKVPAMMEEPSKKRKTARTKMFNNKTLSKQQTLNKDMLPEDSVLNEILQSSYQSALAFDRCTETGLPRTGKMLSSKKKKKFSQYDKFIQNQKEDXSFSRTLSNVNPSDSESSTVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDY6</accession>
    <name>A0A1E5RDY6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Vacuolar protein sorting-associated protein 45</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1941</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDY6">
        <property type="interactions" value="849"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDY6"/>
    <dbReference type="InParanoid" id="A0A1E5RDY6"/>
    <dbReference type="OrthoDB" id="10266265at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="486"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="487"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="669" mass="77200" checksum="04FB33202B8E1D13" modified="2017-01-18" version="1">MDLXKVADYYIDKIVNTTSKKLQANGSTNNGKLVTSHIKVLLLDDTTVQTISMCTTQSKLLEHEIXLVDKLENQVNQESMRQLRCICYFEPSEETVDLLCAELRNPKYGEYQLFFNNSIDKQQLEKIASSDELECVTAIQDFFQDYLIINRHFFSLELSAVQLFKNDGVHKIWRSQYELNSVVDKVLSCLLSLKVTPMVIYEKSNDSVGKLLAESVANKMQSSHKTLFESNKKNKDSVPLLIILDRFQFDPITPLLQPWTYQSMVKEYLNIERNVVDMENSIGEDLDDEDEELKKIVLNCRQDPFFEKTMFSNFGDLSDQVKSYLNDYKKQVEMRKNKIDDLQDIKKFIANYPEFKKTSFNVTKHMTLVGALDNFLKSQQIWSISELEQTISTTNMSLLEEDYQTMLGMLQDPKVEERYKFKMVVIYISVYYMLQKQQQEQQKNGQVTLKMDSHVIALMDTIKPLFNVNDVNFLYHLIKKFTAAPTRRGSSGISGSGSLSGHAGSGHSRARGSSTTSNLNNNMHENLNSTITKSFNESDLFSGLAKKFNQTIHMKDTRPEAENVYMQYQPKLKSLLYDLNGHNLQKMGFYDKYHIVNGNKNTNKLDLPQDVIIYIVGGVTFEENRIVEEFNKDKKKNHNMRCILGGDKIYNTFEYLDSLKTLYNVGNEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDZ6</accession>
    <name>A0A1E5RDZ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Monopolar spindle protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MPS2</name>
        <name evidence="12" type="ORF">AWRI3579_g1906</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Component of the spindle pole body (SPB) required for insertion of the nascent SPB into the nuclear envelope and for the proper execution of spindle pole body (SPB) duplication.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
            <location evidence="10">Cytoskeleton</location>
            <location evidence="10">Microtubule organizing center</location>
            <location evidence="10">Spindle pole body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus membrane</location>
            <topology evidence="10">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the MPS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RDZ6"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071988">
        <property type="term" value="P:protein localization to spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030474">
        <property type="term" value="P:spindle pole body duplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031433">
        <property type="entry name" value="Mps2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17060">
        <property type="entry name" value="MPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="346"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="110"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="153"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="258"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ85129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="458" mass="52706" checksum="AF72EC2C9CBB8D86" modified="2017-01-18" version="1">MNQCDELANELVNDVWSSFTNEPFLYGKDLSKFFCLCGFKSKYGKLSEETIAKLEQFXKGKQYMKIQKEYLSKLFKDITNETLESALGFDTDFGNDASFSKLLATRKGKQNESKITNSLENHTKPVETNSXPLYNPKLRSPFSSYSLKNIGSQEGIQRPKISAHRRNNSGSRFSSAGSTSSVSSNESVQSKHRRTASLPYFNNHNXVNNLVNNPNEKELGVQTSLDGLKLNFLDKRRLSPSSIKKNVEDRGTKMKNMLNEKDEQIYFLETELKRMKNEKMGLQSQYEYLTKEFEAFKKQYVVNSLLAEKLKEQSEMIDLLQRKLNPSRSYSKMSTHNLKTKNKSKFWGFSQAVPLLVVCFALVVFFICFRLSSMPFFLNNTKAAEIDSINASYNSVLASSSSSSSSLSPNYQLKWFEKYAILTKLYWSIRDLFEPQYIAPINDQNFQNFVYEKLFDLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE76</accession>
    <name>A0A1E5RE76_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1997</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RE76"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="81"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="222"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="89"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="472" mass="54342" checksum="2522E535F442EEA5" modified="2017-01-18" version="1">MKHLWSRKNKGDIFGVNHTSLATPFVMSEQTKCISSREETQSESPILEPKVQDDATTYADIIEQPSEAKIPFSEFIVVSDGESTNSKSSTKDRTIKQSDMKSGETRNQMTENTDESLDKKRPENLHFVSHKTFFPSKCALVTLLNKSNMNVKESSTNGAESSIENSPTENVNLQFQVNYETQKELQKKRAMDDSRRKCQNKSSFAENSCLAKADTESMRTQLEVLLVNEQEYINKIASLERQXEQQQEAKLKFANEVHNKQAEGETKESNKMIKLLESFKKQCADLELKNKKLEADFXYAHNKSVSLTEKTEKLEKKVKEXENIINQLTGNNKALIEQKGIKATNQTEAAYEELQEKEFVHKNCKTLRKNIXELKQETATLTSSLKIKESEQAFMISEFQKLKNLLENHQTKLNQVRSKLSWSRSNNYKLQQSYHKLKLENXKLEKLLISNQEDVETWSKMLKSMNSQLEQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REH2</accession>
    <name>A0A1E5REH2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Phosphoribomutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g1669</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REH2">
        <property type="interactions" value="323"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REH2"/>
    <dbReference type="InParanoid" id="A0A1E5REH2"/>
    <dbReference type="OrthoDB" id="8300170at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008973">
        <property type="term" value="F:phosphopentomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05799">
        <property type="entry name" value="PGM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000035">
        <property type="entry name" value="Pgm3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45745:SF1">
        <property type="entry name" value="PHOSPHOGLUCOMUTASE 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45745">
        <property type="entry name" value="PHOSPHOMANNOMUTASE 45A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="11">
        <location>
            <begin position="48"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="12">
        <location>
            <begin position="258"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="13">
        <location>
            <begin position="374"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ85274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="654" mass="75419" checksum="709F56D37153BFAF" modified="2017-01-18" version="1">MPFPKQLQEKIDLWLEYDQNEQTRNEILELCKNPENEEELHKRMDKRIQFGTAGLRSSMEAGWSRMNTLTVLQASQGLCSYIKKTEPTEKHSIVIGHDHRYHSKEFAEITAKCFLLQGFKVYYLNYCGTKPDTDVFCHTPLVPFAINTLKAGCGVMVTASHNPANDNGFKVYYKNGCQIIPPHDNLISEEILQELKPQEHIWDSFEDVVDSNKKKFFDVKDELLSKYIEKIAKELVHIPLHKKKTTSETPTXNNKQKKXNHKPWFVYTPMHGVGYEVFKALMDKLGLYENEDYLVVEEQKIPDPAFPTVSFPNPEEKGALDLSMELAKKHKIRLVLANDPDADRFSVASATFNKDAELFHSNEHHGLTYSQLNGNEIGYLFAYFTFCENHVKVAKGXDGRKKFQMGMLNSTVSSSLIKYMAQIEGFVYKETLTGFKWIGNKALELEKNNPGLVVPFAYEEAIGYMFPKISYDKDGISALVVFLQLYVRFQGSLDMTLNEIYEKYGFYKQHNDYYILPNPKVNNEVFAYIREHTSKKETLNLQIYDLNQAPIKDDCFVMKSFRDLTLGLQTGREEPDLPVDPNSEMITFWFSTKSSENHLKVTLRGSGTEPKLKVYIECMNTSNAVEAQRYADYMWESIKAVWIRPDVTGLHTRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REJ9</accession>
    <name>A0A1E5REJ9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Altered inheritance of mitochondria protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2177</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane raft</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AIM3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REJ9">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REJ9"/>
    <dbReference type="InParanoid" id="A0A1E5REJ9"/>
    <dbReference type="OrthoDB" id="3973404at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031370">
        <property type="entry name" value="Aim3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17096">
        <property type="entry name" value="AIM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="53"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="362"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="430"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="59"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="130"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="164"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="223"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="267"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="292"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="310"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="370"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="455"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="486"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="518"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="569"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="598"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="671"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="704"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="767"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="844"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="863"/>
            <end position="877"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="904" mass="97372" checksum="1AABA7E03B842BED" modified="2017-01-18" version="1">MSSSKNESSSQKSSKGESGDGNFWDNNGGAIMSATSSVLHAGYRTTRYVAKAGYKAGKNHYEQSKNKRNGEEKSGSSESLETVQSPPMGASQDVRQFQPPPLRAGQKQYQPGGVIVDAGNTPMANVSPVPATNTYSGTPQQGYPNAGYVQTNVPQPXAGGVQNFAPQAGTSSPQIFQPLYGADGQIIGYAPMPSQVGSAQPPAPAPTHPQTXXSFVQQENSNQQPQQYQQPQQYQQPQQYQQYQQYQQYQKPQQYQQTQEQISQPQIPQPQIPQPQIPQPQLPQPQIPQPQIPQQQQYVHPAQRPPQPSPEQQQPAPLPARAQPAPPPTAPRLAVQIPGSNSAXLASGPALVAAQPLPYRAAAEFPPPNVQTQPFSPQLGENISTNPQTADEGELSSQSSNGPMYKVTPYEFMDPEQRAEAKRIELKKVDITTFAPPPTHAGRKTLTGPKLSQLIEDKQSGRITSEEFNSRSGSSSTGPVKKLGRSTTNSSWNSSQSSINLPEPNYPSKPTEYPVVAPQQSTPVSPHTSGIEQNVARLSLDESNSAAAVDSEAVPPPPYTVENSAKPLTPTSTNAAGTAGALNSQRTVRPSHSAAPPRNSTLSIKNQRTFSPNASSTNTEGQAVNKATVLKGNYDYQKPVNFXPPPRPHRNAQDLENEEANKNRVTANKYSRMAASSSRTSRAXAPPQRSHRVLQNAEPEPIVNTPSNPSLSSPQANLPSRNDFPPPPKRVIQSSESEGNPHVAQTPLGISEEADMFPKISRLPSRPQFSTQGRSDNLSSISVPSNSPKAGPPIIKPKPEALKGQPPAKPFKPTYLTDRSESSLSSLNENAGGGNIDFVELAKNKTKKKAPPPKPKKKPTLSGVVSSMDQTNGTSGXKNDDYNPFSVYSKGSVPAELDHLQHKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REN9</accession>
    <name>A0A1E5REN9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pre-mRNA-splicing factor BUD31</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1707</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BUD31 (G10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REN9">
        <property type="interactions" value="923"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REN9"/>
    <dbReference type="InParanoid" id="A0A1E5REN9"/>
    <dbReference type="OrthoDB" id="277109at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001748">
        <property type="entry name" value="BUD31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19411:SF0">
        <property type="entry name" value="PROTEIN BUD31 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19411">
        <property type="entry name" value="PROTEIN BUD31-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01125">
        <property type="entry name" value="BUD31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00322">
        <property type="entry name" value="G10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="133"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="139"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="182" mass="21104" checksum="2ACE0243AB5CD5A5" modified="2017-01-18" version="1">MKTPPPQGYEKIQKTLDSYAIELKNITTKNSSTNDXKQKLYTSSNQDFWEISRIAHKRSRYIFDLFYKRKLISKELYQWCLDQGFADKHLIAKWKKRGYEKLCCLKCIQRVETNEGDKACICRVPRATLLKQQQQQQNDKSDDGNEENPKSKNETTQSSILNTKIAFKQCQNCGCSGCASTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REP6</accession>
    <name>A0A1E5REP6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">endopeptidase La</fullName>
            <ecNumber evidence="7">3.4.21.53</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1874</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase S16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.53" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REP6"/>
    <dbReference type="InParanoid" id="A0A1E5REP6"/>
    <dbReference type="OrthoDB" id="2411602at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19500">
        <property type="entry name" value="RecA-like_Lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000021">
        <property type="entry name" value="Lon protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.5270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054594">
        <property type="entry name" value="Lon_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008268">
        <property type="entry name" value="Peptidase_S16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="ATP DEPENDENT LON PROTEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22667">
        <property type="entry name" value="Lon_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01046">
        <property type="entry name" value="LON_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 8 10" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Lon proteolytic" evidence="9">
        <location>
            <begin position="781"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="876"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="919"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1016" mass="113988" checksum="6FEECB08EEE2F3B1" modified="2017-01-18" version="1">MTNSSKSQNKLKFPAVSLLKHNDSINLPGTTYEYKLPKKFILEKMFAQYKATSFFKRNFIIKNLGVSASNETPIDDFCFQTVQNFHENISANAGKXGLVISNHDSSSAQAQKNAVQETPLYIALITHKHGKAPKDWLYGTVAKIQSCVVHSXEEYVLLKFKSMARIKGRVSSSFELEKAXGLFILHDVVFEEQLTCSQXXGQSKWKSKLIDTVKETNGLIKXFVERYENSAKRKNNNSFHLLSPLNNVLYVYLTRTFFKSAWATVLGLVAIADTSELYALSDLLMSILPIARFQKIRFLQEFLMEGKLSFCVELLQKQFNFLLRNLYEVDEYTKSSYQTMTKSEKARVLAAQLKSLKFCLDDLKLLPRSKQPDNENYNSGAYLSEEGKDSGQLDEIRGFMKKLTEKGVHXEGQQLLERDFKRLAKMNPQSAEYQQLQNYFDNVMDIPFGRXDESPTRSGFDLAKARVILDEDHYGLTKIKTRLIEYLCIHNILQNESFGQKAKDMEKQHANPTILLLDGPPGVGKTSIAKSIAKVMHRNFQRISLGGIYNESDIRGHRRTYVGSMNGVIINALKKAQSMNPVILLDELDKIFTGVNGNMISSNNGNPSAALLEVLDPQQNSTFMDHYVGFPVDLSQVVFICTSNDCQNIPAPLMDRMEVIELSGYLFDEKKQIALRHLLPKQISLNGXDRFENFQKLDITEXAWKFLICSYTXEAGVRGLNRLLGAIVRSCVVQEVXKKSLETCGTITIDKEYLLKLFGETRDYNNQTGDQSLENALTHESDKLGIVHGLSYNSNGTGSVLTFEIIKIGEVATKTGPLITHTTGNLGNVLLESITIGCSIVKNLMLSGVINKNTELLKSEYHLHVPEGAISKDGPSAGMAITVALLSYALNKPVKNTVCMTGEITLQGHILPIGGLREKLYGAAMNGMETVLAPLQNSGDLRNFCVDNAWSSPDIYNFPKDDYRELRVIKERLGLQVHYVSTIHDAISLIWENAETSPHTFCPNEKAPQHPIPSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RER2</accession>
    <name>A0A1E5RER2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2426</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RER2"/>
    <dbReference type="FunCoup" id="A0A1E5RER2">
        <property type="interactions" value="1280"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RER2"/>
    <dbReference type="InParanoid" id="A0A1E5RER2"/>
    <dbReference type="OrthoDB" id="5778525at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11403">
        <property type="entry name" value="bHLH_scINO4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057072">
        <property type="entry name" value="bHLH_INO4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23181">
        <property type="entry name" value="bHLH_INO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="2">
        <location>
            <begin position="48"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="143" mass="16463" checksum="8ADAB9A2C2DE4003" modified="2017-01-18" version="1">MANVQNASESKDISNTAAQLRESKENSLSKGTKKVPKRWKKPLLTDNVKKKNHISSEHRRREMIKQKHDSLIKLVPALSKDPEQVKGELIIYPKTYEYLVALYEENLRLRKLLANSLVSHSDSSGDKNIIIREELIWEKPDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REY4</accession>
    <name>A0A1E5REY4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Protein transport protein BOS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g1967</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">SNARE required for protein transport between the ER and the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 12">Belongs to the BOS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REY4"/>
    <dbReference type="FunCoup" id="A0A1E5REY4">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REY4"/>
    <dbReference type="InParanoid" id="A0A1E5REY4"/>
    <dbReference type="OrthoDB" id="158360at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027027">
        <property type="entry name" value="GOSR2/Membrin/Bos1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230:SF1">
        <property type="entry name" value="GOLGI SNAP RECEPTOR COMPLEX MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230">
        <property type="entry name" value="VESICLE TRANSPORT V-SNARE PROTEIN VTI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12352">
        <property type="entry name" value="V-SNARE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028865">
        <property type="entry name" value="Membrin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="111"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR028865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ85469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="257" mass="29411" checksum="C69EA4E5D4CFE667" modified="2017-01-18" version="1">MSHATKQKQLLTKDLSKLEENIVSAPISLQGSITTTLLSLEKTIVKYKQQLENFKQTSNIGAVVQDEEETAELLKYETRLNILESEYANFKDQFTLLKERYQEEQSKLLQTPNNGINTMQGKSSSSTNPFTDTSNTTMTNRRTQGHLKDFGDGSGTGQLNTDYQDSLRNEESILARGNQKLDYILEMGQHSLDDIMDQNQVLEKMQKTMTKSLSTLGVSNETIERINKRVFKDRLVFYIALILFFVGVYLVLKFLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REZ1</accession>
    <name>A0A1E5REZ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sporulation-specific mitogen-activated protein kinase SMK1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2157</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REZ1">
        <property type="interactions" value="293"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REZ1"/>
    <dbReference type="InParanoid" id="A0A1E5REZ1"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000040">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="99"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="450" mass="51157" checksum="C3230F9BD79F9CCB" modified="2017-01-18" version="1">MNNQGYFVKNTLQNNPQIINNLSFTPKNGAQGXKTQGFGNIESQNQXKEKQNFKAKNPVASCNKXXENGNASIGISQKADCSVKMTTYEKFSFMVPEKYEIQQVLGKGSYGVVCSALDRTDPEALAPIAIKKITNIFTRDILVKRAIRELKFMRFFKGHKNIIGLVDLELVTEKPYDGLYCFQELLDYDLARVIHSSVQFSEFHIQSFFYQILCGIKYLHSADVIHRDLKPGNILCTIQGTLKICDFGLARGISSQLLGQMTTETGRTHHITNYVATRWYRAPELILTRTNYSKAIDIWACGCILAELYGRKPVFIGNDHMHQXAEILKVLGTPSNDDLLSYGSRVAWELFCPPKRQHKKIAWSQIYPFASKNGLDLLEKLVLWNPEARLKVEQALEHPFFKDVKNPNDEPGCPYGVFDFQYEHHLESIDSLVSCLKKEVENFKSGKTSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REZ7</accession>
    <name>A0A1E5REZ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2418</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5REZ7"/>
    <dbReference type="OrthoDB" id="4064992at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="149" mass="16313" checksum="CD4067511B5BE43B" modified="2017-01-18" version="1">MSSSSSAFIPKNFGNSGSGRPSAYGRNSNAKFHALEQKLFGPSIDDLNANNPKLLSLNLLLSALLLVLTSGLVFLLGXNIVIQYRTMPPSGPGLVRNACYLVLSFFAIMALQKFNVYTYRKLAGPIKQNLRAYTRLGGGESYELHERTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF03</accession>
    <name>A0A1E5RF03_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small nuclear ribonucleoprotein Sm D2</fullName>
            <shortName evidence="9">Sm-D2</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">snRNP core protein D2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1994</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the snRNP core protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF03"/>
    <dbReference type="FunCoup" id="A0A1E5RF03">
        <property type="interactions" value="1221"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF03"/>
    <dbReference type="InParanoid" id="A0A1E5RF03"/>
    <dbReference type="OrthoDB" id="437526at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030532">
        <property type="term" value="C:small nuclear ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01720">
        <property type="entry name" value="Sm_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027248">
        <property type="entry name" value="Sm_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12777">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9 11" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="35"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="116" mass="13400" checksum="156088F1FC793D46" modified="2017-01-18" version="1">MSHSAYRDLSNVSRSELTPNELKKLEKYEFQHGPLSLINEAQVSKTPVIISLRNNHKIIARVKAFDKHCNMVLENVKELWSEKHHKNGKLIPRERFISKLFLRGDSVIIVLKAPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF06</accession>
    <name>A0A1E5RF06_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Mannan endo-1,6-alpha-mannosidase</fullName>
            <ecNumber evidence="4 12">3.2.1.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g2091</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Required for normal synthesis of the cell wall.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>Random hydrolysis of (1-&gt;6)-alpha-D-mannosidic linkages in unbranched (1-&gt;6)-mannans.</text>
            <dbReference type="EC" id="3.2.1.101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the glycosyl hydrolase 76 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.101" evidence="4 12"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF06"/>
    <dbReference type="FunCoup" id="A0A1E5RF06">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF06"/>
    <dbReference type="InParanoid" id="A0A1E5RF06"/>
    <dbReference type="OrthoDB" id="4187847at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008496">
        <property type="term" value="F:mannan endo-1,6-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007117">
        <property type="term" value="P:budding cell bud growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000006">
        <property type="entry name" value="Mannan endo-1,6-alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005198">
        <property type="entry name" value="Glyco_hydro_76"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014480">
        <property type="entry name" value="Mannan-1_6-alpha_mannosidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12145">
        <property type="entry name" value="MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DCW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12145:SF21">
        <property type="entry name" value="MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DFG5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03663">
        <property type="entry name" value="Glyco_hydro_76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016302">
        <property type="entry name" value="Man_a_manosd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184742" description="Mannan endo-1,6-alpha-mannosidase" evidence="14">
        <location>
            <begin position="28"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="450"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR016302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ85481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="472" mass="51667" checksum="5FF493BE35DAE944" modified="2017-01-18" version="1">MILSKVPFLALFTVLSITSLYSNLAHALSLDPTSKTSICSDTALIEKGMLDYYEGTRYGGTVGYFQAPYYWWESGEGFGAMIENWFVCENDTFTTLLQEALIHQASSSFDFMPENQTMVEGNDDQGVWGLTLMTASERNFTRASNGTLGVKNIPDWVDMAQAVYNTMYSRWDTSSCGGGLRWQIFTWNNGYNYKNTVSNGCLFQMAARLGRYFGNDTYLDVAEQVWDWLVDVGFVNLSSDSKIWDGATIDDNCTSDVTKIEWSYNYGIILGGAAYMYNATNGSSTWESRVTQLLDGAVSAFFNDDGIMYEQQCQASKSCNNDQRSFKTLFSANMGYVSVMAPFTASTVDPLITTSAVAAAGSCDGGYDGVTCGMNWFNATNDGYYGLGEQMCALSVIQTLLVHDVDAPYQVVTNEYMAQGDSEAGLSSTSSNNEASLLNAKLDISHGDRVGASILTAVILLILITGSISMLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF09</accession>
    <name>A0A1E5RF09_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C-1-tetrahydrofolate synthase, cytoplasmic</fullName>
            <ecNumber evidence="8">1.5.1.5</ecNumber>
            <ecNumber evidence="7">3.5.4.9</ecNumber>
            <ecNumber evidence="6">6.3.4.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">AWRI3579_g2132</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>(6R)-5,10-methenyltetrahydrofolate + H2O = (6R)-10-formyltetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="3.5.4.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:23701"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="22">
            <dbReference type="Rhea" id="RHEA:23702"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-methenyltetrahydrofolate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22812"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:22813"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="20">
            <dbReference type="Rhea" id="RHEA:22814"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>(6S)-5,6,7,8-tetrahydrofolate + formate + ATP = (6R)-10-formyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20221"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:20222"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="21">
            <dbReference type="Rhea" id="RHEA:20223"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.5" evidence="8"/>
    <dbReference type="EC" id="3.5.4.9" evidence="7"/>
    <dbReference type="EC" id="6.3.4.3" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF09"/>
    <dbReference type="FunCoup" id="A0A1E5RF09">
        <property type="interactions" value="1069"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF09"/>
    <dbReference type="InParanoid" id="A0A1E5RF09"/>
    <dbReference type="OrthoDB" id="5126881at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004329">
        <property type="term" value="F:formate-tetrahydrofolate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00477">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000006">
        <property type="entry name" value="Bifunctional protein FolD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000245">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001123">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000005">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.410.10:FF:000001">
        <property type="entry name" value="Putative formate--tetrahydrofolate ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.410.10">
        <property type="entry name" value="Formyltetrahydrofolate synthetase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01543">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000559">
        <property type="entry name" value="Formate_THF_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020628">
        <property type="entry name" value="Formate_THF_ligase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020867">
        <property type="entry name" value="THF_DH/CycHdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF26">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01268">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00721">
        <property type="entry name" value="FTHFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00722">
        <property type="entry name" value="FTHFS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00766">
        <property type="entry name" value="THF_DHG_CYH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00767">
        <property type="entry name" value="THF_DHG_CYH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0436">Ligase</keyword>
    <keyword evidence="19" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="17" id="KW-0521">NADP</keyword>
    <keyword evidence="13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic" evidence="23">
        <location>
            <begin position="60"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding" evidence="24">
        <location>
            <begin position="197"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00017592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00051435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00051544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00052163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF02882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="OEJ85490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="999" mass="107806" checksum="1FE1CFDFCC33C85B" modified="2017-01-18" version="1">MLQASTKRAAGQNVKRVFNNCNKQLSSVSIAAVQKTSKTTFSSVYSNFSSFSQSRLYHLLSGKEIAKRIRLESNDEIQKIKQDVPEFKPFLKILQIGARPDSSTYVKAKLNASNESGVACDVEKLPENISEIELLEKIKDINNNDSIHGLLIQLPLPKHLNETKITNAVSANKDVDGFHRYNVGELSKRGGEPYFIPCTPNGCLKLLKETGIPLKGKNAVIIGRSDIVGTPVSSLLKNENCTVTTCHRYTENLPQILQNADIVVAACGQPNFVKGDWLKQDAVVIDVGINYIPDSSKKSGRKLVGDVDFESCKNKASFITPVPGGVGPMTVAMLVKNVLQAAKTAESQNNKLPTCEPLPLKLETPVPSDIAISKKQTPKHISIVAQELGIHENEIEFFGHYKAKVNPKILNRLDQNNKTNGKYVLVAGITPTPLGEGKSTTTMGLVQALGAHLGKPAIANVRQPSMGPTFGVKGGAAGGGYAQVIPMDEFNMHLTGDIHAISAATNLLAAAIDTRMFHESTQKKDSVFYKRLIPVKKGTRTFTKSMLKRLAKLGINKTNPDDLTPEEINKFARLNINPDTITIKRVVDVNDRLLRQITIGQAPTEKGFTRQTGFDITVASELMAILALSKDLKDMRERIGRIVIGANFNNEPVTAEDVGVAGAVTALMKDAIKPNLMQSLEGTPVMVHAGPFANISIGASSVIADRIALKLVGSSSSLSSDKKTQDSGYVVTEAGFDFTMGGERFFNIKCRASGLSPDCVVLVSTVRALKLHGGAPDVKPGQPLPAEYVQENVDLVRKGAANLQKQIANAKQFGVPVVVAINKFESDTDAEVEAIKAAAKEAGAFDAVPSNHWAEGGKGAIDLAECVVEATAQPKNFEFLYDVDQSVEDKLTTIVQKMYGGAEIEISDLAQKKIDMYTKQGFGKLPICIAKTQYSLSHDPALKGVPTGFKFPIRDVRASIGAGYLYALAAEIQTIPGLSTAAGYLNVEVDEDGEIEGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF76</accession>
    <name>A0A1E5RF76_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1755</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL36 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF76"/>
    <dbReference type="FunCoup" id="A0A1E5RF76">
        <property type="interactions" value="920"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF76"/>
    <dbReference type="InParanoid" id="A0A1E5RF76"/>
    <dbReference type="OrthoDB" id="9616667at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1760:FF:000003">
        <property type="entry name" value="60S ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1760">
        <property type="entry name" value="60S ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000509">
        <property type="entry name" value="Ribosomal_eL36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038097">
        <property type="entry name" value="Ribosomal_eL36_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10114">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01158">
        <property type="entry name" value="Ribosomal_L36e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01190">
        <property type="entry name" value="RIBOSOMAL_L36E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="115" mass="12742" checksum="A897B98DB575E285" modified="2017-01-18" version="1">MAAKTVHKFQTQDITSHIQTGIASGINKGKKVNQMTPAPKVSYRKGALSKRTSFVRSLVKEVAGLAPYERRLIELIRNAGEKRARKVAKKRLGSFGRAKAKVEEMNNVIAASRKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF92</accession>
    <name>A0A1E5RF92_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">fumarate hydratase</fullName>
            <ecNumber evidence="2">4.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1804</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reversible stereospecific interconversion of fumarate to L-malate. In mitochondrion, catalyzes the hydration of fumarate to L-malate in the tricarboxylic acid (TCA) cycle to facilitate a transition step in the production of energy in the form of NADH. In cytoplasm and nucleus, involved in DNA repair in response to DNA damage: following DNA double-strand breaks (DSBs), translocates from the cytosol to the nucleus and promotes DNA repair by catalyzing the dehydration of L-malate to fumarate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.2" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF92"/>
    <dbReference type="FunCoup" id="A0A1E5RF92">
        <property type="interactions" value="731"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF92"/>
    <dbReference type="InParanoid" id="A0A1E5RF92"/>
    <dbReference type="OrthoDB" id="1738025at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004333">
        <property type="term" value="F:fumarate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006106">
        <property type="term" value="P:fumarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01362">
        <property type="entry name" value="Fumarase_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.40.30:FF:000002">
        <property type="entry name" value="Fumarate hydratase class II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.10:FF:000001">
        <property type="entry name" value="Fumarate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000001">
        <property type="entry name" value="Fumarate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00743">
        <property type="entry name" value="FumaraseC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005677">
        <property type="entry name" value="Fum_hydII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018951">
        <property type="entry name" value="Fumarase_C_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00979">
        <property type="entry name" value="fumC_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008909">
        <property type="entry name" value="PRK12273.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11444">
        <property type="entry name" value="ASPARTATEAMMONIA/ARGININOSUCCINATE/ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11444:SF1">
        <property type="entry name" value="FUMARATE HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10415">
        <property type="entry name" value="FumaraseC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarate lyase N-terminal" evidence="5">
        <location>
            <begin position="45"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarase C C-terminal" evidence="6">
        <location>
            <begin position="442"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00056821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="498" mass="54198" checksum="C3C098EEC22E23F3" modified="2017-01-18" version="1">MLRNTSKLVNSNILKSTQQSILTKRMSSNSSGSTGQYRIETDAFGEIKVPADKYWGAQTQRSFENFKIGGIREKMPEPVIKAFGILKKSAAVVNTELGTLDPEISKLIQQAADEVISLKLIDHFPLVVFQTGSGTQSNMNANEVISNRAIELMGGEMGSKKIHPNNHCNQSQSSNDTFPSVMHIAAVLEIEQKLIPALGELKNSLELKSQEFDKIVKIGRTHLQDATPLTLGQEFSGYVQQLENGIKRIEHSLINLKFLAQGGTAVGTGLNTKIGFDTKIAHQVSLETGIEFKTAPNKFEALAAHDAVVECSGALTTVACSLFKIAQDIRYLGSGPRCGYGELSLPENEPGSSIMPGKVNPTQNEALTQVVAQIMGNNTTITFAGSQGQFELNVFKPVLISNLLSSIRLMTDASHSFKIHCVDGIKANKDKIEKLLNESLMLVTALNPKIGYDMASKVAKNAHKKGITLKESALELKALSEEEFDQYVVPEKMIGPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFB1</accession>
    <name>A0A1E5RFB1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">DNA repair protein RAD14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1743</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFB1"/>
    <dbReference type="FunCoup" id="A0A1E5RFB1">
        <property type="interactions" value="106"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFB1"/>
    <dbReference type="InParanoid" id="A0A1E5RFB1"/>
    <dbReference type="OrthoDB" id="5368863at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000110">
        <property type="term" value="C:nucleotide-excision repair factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901255">
        <property type="term" value="P:nucleotide-excision repair involved in interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000715">
        <property type="term" value="P:nucleotide-excision repair, DNA damage recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.530.10">
        <property type="entry name" value="XPA C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000465">
        <property type="entry name" value="XPA/RAD14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022656">
        <property type="entry name" value="XPA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037129">
        <property type="entry name" value="XPA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022652">
        <property type="entry name" value="Znf_XPA_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00598">
        <property type="entry name" value="rad14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10142">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-A CELLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10142:SF0">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-A CELLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05181">
        <property type="entry name" value="XPA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00752">
        <property type="entry name" value="XPA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="XPA C-terminal" evidence="11">
        <location>
            <begin position="240"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="22"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="40"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="71"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="12">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ85600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="389" mass="44534" checksum="0E9B47BEC828A0AC" modified="2017-01-18" version="1">MNLNAEQKASIEANRKRALERLQKRGISIPSSSKLHETSSKVTKPSIQGGTEHSKGNAANSTQATKVVESTAVSGPNDSPQQPSSSTHIESHPQNRDASSVETKKQYIRPSIRKKDYIEYDFSTMENSYGGFINNDRSLYGSGYSSIDNAEKTFEQWQAEQRSKNDLFNGPSSSTEPGDAVENGTQLVREFKHLGPDIISDLSALPKCEECHVNVEFDTILLQHFKIKICKRCTVEHPEKYSLLTKTECKSDYLLTEPELEDHKLFHRLEKANPHSGTFARMQLFLRKEVEEFAFEKWGGQDGLDKEWEQREASKLERREKKYQLKLKEMRKKTRAQEYTQKMKMKKFGAKHIHEFSAPLKMKKKSDDGYDVVKRRCIGCGMEVEELDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFF2</accession>
    <name>A0A1E5RFF2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcription and mRNA export factor SUS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2183</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFF2"/>
    <dbReference type="FunCoup" id="A0A1E5RFF2">
        <property type="interactions" value="355"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFF2"/>
    <dbReference type="InParanoid" id="A0A1E5RFF2"/>
    <dbReference type="OrthoDB" id="6221744at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018783">
        <property type="entry name" value="TF_ENY2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038212">
        <property type="entry name" value="TF_EnY2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12514">
        <property type="entry name" value="ENHANCER OF YELLOW 2 TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10163">
        <property type="entry name" value="EnY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0811">Translocation</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="103" mass="11709" checksum="8AE0DA47678F5100" modified="2017-01-18" version="1">MTSPTTQSDVELREQIQNVLIKSHMYEQFNTELRSKLLQDGWIDRVKKMAIDEIMKQDGGSATDKSFSDILSAIEGPALNDVPEDIKTEMLSKFKSFLNDVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFG8</accession>
    <name>A0A1E5RFG8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Ubiquitin-like protein ATG12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1777</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Ubiquitin-like protein involved in cytoplasm to vacuole transport (Cvt), autophagy vesicles formation, mitophagy, and nucleophagy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Forms a conjugate with ATG5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Preautophagosomal structure membrane</location>
            <topology evidence="1 7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ATG12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFG8"/>
    <dbReference type="InParanoid" id="A0A1E5RFG8"/>
    <dbReference type="OrthoDB" id="10003551at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034274">
        <property type="term" value="C:Atg12-Atg5-Atg16 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019776">
        <property type="term" value="F:Atg8-family ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061723">
        <property type="term" value="P:glycophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01612">
        <property type="entry name" value="Ubl_ATG12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007242">
        <property type="entry name" value="Atg12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13385">
        <property type="entry name" value="AUTOPHAGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13385:SF0">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN ATG12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04110">
        <property type="entry name" value="APG12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <keyword evidence="5 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="125"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="68"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="125"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="288" mass="31695" checksum="CA524AD8A3B39E2B" modified="2017-01-18" version="1">MSFQNSNVSNKKEQSXAKPNEKAHHLASMIEILQNELDGLQDNDSDSESSSSEAKSEKKQETDVNCGETETVQTTQLSSTHTRKKGEPSFSPAKEEHMPRNLSNEFAKIDLSKFSNVEDTQNKVSTSHHSSENVSSXVAQVQPGRPGIPGTQYTVNIENPXVSSDNEAHPAGKLVERDLSPLAAVTPINIDLSQSPXLMLPNPAGNKVKIRFSPIGSITPLNKMVINASQPFSVIINFVKRKTNRKHVYLYINNSFAPSGQQLVGELYEHFRINDELWVSYCGGVAFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFQ9</accession>
    <name>A0A1E5RFQ9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Galactose/lactose metabolism regulatory protein GAL80</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2193</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFQ9">
        <property type="interactions" value="357"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFQ9"/>
    <dbReference type="InParanoid" id="A0A1E5RFQ9"/>
    <dbReference type="OrthoDB" id="64915at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055080">
        <property type="entry name" value="Gal80p-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051317">
        <property type="entry name" value="Gfo/Idh/MocA_oxidoreduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43708">
        <property type="entry name" value="CONSERVED EXPRESSED OXIDOREDUCTASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43708:SF1">
        <property type="entry name" value="GALACTOSE_LACTOSE METABOLISM REGULATORY PROTEIN GAL80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22685">
        <property type="entry name" value="Gal80p_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="7"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Gal80p-like C-terminal" evidence="2">
        <location>
            <begin position="148"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="457" mass="51477" checksum="B90931632233066A" modified="2017-01-18" version="1">MQRSKLRIGFIGLSGKKGWAMKSHFTVIQNLSDYFEIVAISNSSMEASCKTIEELRLSNCKPFGNDYNSFVQYDQLDMVVVSVKVTTHYNIIMDLLNFKYKNPNLCTFKILLVEWALGNGLKEASNIYELCNKTNVQSVISLQGRYCPHMLLAKKLIGENRIGKINSIDVTANAGWYGYQRPYNSPDYLYDIHSGHNMITTAFAHTIDSIQYIVGAYVQRLNAMIFNNIKQQEVIGPKDVSTGVFKPKTVPDHVLVQGSISATENIPISVSFQGGTPTNNFTKNLVINIHGTKGDLKIEGNAGFPQISNLALYFCGVPPTNGKEFTSETNTFGPNDLQYNNKTTLENKGCNSQPYNLMDPQAKGGMPFPQNSQFSXQDEEKHQVVEKYQLNNYSPVVGNMMRLYEAILRYYNHDTLYQAQEFATEGFPTFKDAVLLHRLIDSVFESSEFGRTVDCQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGI2</accession>
    <name>A0A1E5RGI2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Regulator of Ty1 transposition protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1088</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGI2">
        <property type="interactions" value="77"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGI2"/>
    <dbReference type="InParanoid" id="A0A1E5RGI2"/>
    <dbReference type="OrthoDB" id="342264at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035361">
        <property type="term" value="C:Cul8-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990683">
        <property type="term" value="P:DNA double-strand break attachment to nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053036">
        <property type="entry name" value="CellCycle_DNARepair_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031906">
        <property type="entry name" value="RTT107_BRCT_6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47667">
        <property type="entry name" value="REGULATOR OF TY1 TRANSPOSITION PROTEIN 107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47667:SF1">
        <property type="entry name" value="REGULATOR OF TY1 TRANSPOSITION PROTEIN 107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12738">
        <property type="entry name" value="PTCB-BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16770">
        <property type="entry name" value="RTT107_BRCT_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16771">
        <property type="entry name" value="RTT107_BRCT_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="128"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="540"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="578"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="591"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="607"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="570"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="713"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="741"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1032" mass="117360" checksum="A16964A4AD08B38E" modified="2017-01-18" version="1">MARYPLLEGLEVILITDSTNKIDSKTYDLYKXKLLEXFGCAHVYELDICTQQDDIKSVEQFIACYNKVALCVTVFPPGAADLTAKLVNEFQFYKIXTIDLMIPIVSHQWIEDVLFHGKILKTSRNAINPEYFLQNCNIYINKNEYSEVELHVLQIIISNFGGSVILSTYRSTLVTHILTSNEHLYKSKLERTLIARPEWIISMLLKYRRVEIADSFESDSSLAEFPKVFQGQVFFISDAFRNAFSSIGIENFTSILDTLITKRGGTVHYSSPSSSIYRNAYSFEYKHDAAFDCLPMSNRCNLWWLLDSINYKLGSNKMNXKLLHYPYKALDHLIPQAKSQLLSFTNFIGVQRFYVQELLSCLEIPYTLDFSAKNTSLIYYYKNGPKYKYAKAQNIPTLNHNYLEYCYXFGKIIDYNTFMNNFDNDFXFVNYCGLVGSSFEKDTGKGKAADEEDYTNKQGIGNSEYDGREDSFVDXHVANKQPSSSEKFTQSVINEVLDMASPNVPITEELNNATFAKPLKEIHDVEIVSTKNELMVTTPNKGQNQEANTEKKVEEQNIEXENYDKSVXSDVSPVTKLSSSASIEXNSSRVGDXEKENEQFTKKIHESTQVNSFPSKSTSPSVFVEFDQTNSSVVSQNFANNSLTSSQITHSQRKAKQKAVKKVTKDIGTLNEYQKVAKKKRLDDEGNYTNTSFEVTHNTSFSHKKTXENNFADGDGXVLDDSDEDLSSELRNNLKQLLFEDNNKFRTLLPTGETLICITTGFQMLDYLDRNNLEQDLKLLRAIGVQLVNAPAGEMNSKETDLYNSANCLIAPKKLRTEKFLKALSLRNLRYIILPKFLTQVLTVIKEGNSRHLPSISKYKIPEMDCDNLQKQIQFLVSQNEHISSVSKSHSTNMFSTYGINKINIVQNINGGVPVISDILRAHGIVDVNSISEKVKDIVVDRDVVANEKKRDEKNPSDAPYFKYVFLVNKTSQANSILKALKQQGIISSEKKTKKDGATSEQVLIVEWNWCVKCIFNLSIDFNNKEFVVCMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGU7</accession>
    <name>A0A1E5RGU7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1130</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGU7">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGU7"/>
    <dbReference type="InParanoid" id="A0A1E5RGU7"/>
    <dbReference type="OrthoDB" id="3972174at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055425">
        <property type="entry name" value="IgD1_Trs65"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055426">
        <property type="entry name" value="IgD2_Trs65"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23645">
        <property type="entry name" value="IgD1_Trs65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23646">
        <property type="entry name" value="IgD2_Trs65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trafficking protein particle complex II-specific subunit 65 IgD1" evidence="2">
        <location>
            <begin position="18"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Trafficking protein particle complex II-specific subunit 65 IgD2" evidence="3">
        <location>
            <begin position="238"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="546"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="148"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="4">
        <location>
            <position position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ86139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="700" mass="79016" checksum="A8B7EB823313DDCB" modified="2017-01-18" version="1">MTKNIKENCPAVXLEIFEEHSNCRKFVLFDEILPIRLQNFKKEYKPLNLIVEINGVRVFENGAKLFKDXDSKQDCYHIQNVKQIFQEFVYXNYAVMNQGRDNMIKFVFQYEVREDQIQGNEGVQEXDQTGHLKEVSNGKKFKVASETNSSGPDNDXSNNITNNDLKPQLKNKQKSNLLSFEPISPFANGSMTXEEQNXIVDKHMETKSSIEKETLSLSRIXXHQYKMYETTVSFPIYSVLSFKLKNVALKKQKKVLSSVEIQPSKTLKYLDKIYGTGFDSQDAFLQIKDVSYSLVNESNVKLSPIGFSKDKNIFFENIILKPENEYAFNFEVPYEHNSQVKVFINYKVFGAFWDCGXIDANKSLSETGIDVEVVYKTKIAVERNMSNSLVGLSSFSRTSRTNSVNNTVNNSISNKTFGYNYATTSASSLLLPPAHTLAYDVNHGSEYGNYNRINSNNSISAHNINYMNNTYHNTINSALSNKFXSLIIDKVNETSTASENNTIYAKVGEPFKVKFQLRYENSANSLTELVIYQSVNIPNFTTSAMSTSTGSNTTQYNMGSAQSSRASNPQSGFASANTTTGMTSPIRMSELYMPGNSKKSYSGAAPHSSKSVMNQDIALSNRYYKWKKSLAQLPYQRGIVLLNNDYKISMETISRNTMEFQVELVGLQKGFYPNVHGLRIVNLNTSEITPFGGKLAVMIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH18</accession>
    <name>A0A1E5RH18_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Eisosome protein SEG1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1439</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH18">
        <property type="interactions" value="81"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH18"/>
    <dbReference type="InParanoid" id="A0A1E5RH18"/>
    <dbReference type="OrthoDB" id="3972972at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="387"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="486"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="671"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="213"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="508"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="524"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="552"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="573"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="601"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="635"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="702"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="757"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="850"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="865"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="914"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="932"/>
            <end position="942"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="942" mass="102083" checksum="345306F12641342E" modified="2017-01-18" version="1">MGLFTPSRQRSAANPPYDITQPRSGKPAANSSAMAAASTIGRRYSLTVDPTVGNKPSTGHGRTSRNNSLLNSARASSLNSSATGXKKTITKKKTQENTKSSTVKPSGSKKTYSLQKNXSNLGKMYSSGDQNNKSVYYDAQEAFDDFGGPQAKGNLIKKAKSIQKSSAGNQSGTFKSKPDEASDRKLYKYVPTATGLVMVELSASEESKRLKRSSSIATSMRRSSSNISLNSVPRSSSLTQRTSSTSRSNSATSNSALGPKFHPAQPPLKDAASSKLKRSASMNALKQKKNKNVESDLXAPPPKRSINGTNSSRNSTYSPMKKKLGTSDFIPEETVTELEEAAIEEGRHYKPAPKKTGXSTVRRKTSSNLRINPITVKTAPLATKNTEKTPTTVANKDLQKAKQTNAEKNSKSTTVADTKKEINVGKSTKAPPSVQKSATAQHSKDNQITKEYSEFQQNAVKNEEPVKVTPEADYLSQPPVINIEEEDFPENRDQLLGTPTKPQVSRDSNYENDKSAATPEEHNSMASLLRSNNSYLNKNGTNNSGHNGNRESTSPPVASTVVTTPQKHVPGSATGSVKRTPSTQRTPMKSAMKKPEIDADSNVSQQNGIQTRRLSQKASSSSLVKNKTPIKSALKKSTTGQSQSNTSAAEQAYLKLTTAENTRLNAQLMDENQPKSKIIRSPSKRLSTLRMKPIVPPAAKPNVNNSNNGQTRTKTSEKRNSTIKPVGNAKSRERSSALDQAIKTASKQAAFQPVASDSEKKSSFERERPSNSNMGFKMLSLRGHEDEEADELAGSALNGSGDLGGRAPKLARSYSRDAEEDDFDKFRKSFKSKFQDSDSDSEILVPPPRNKNRRTSIISLHKKSTNNSVSSSGKNAPVSSSKTQKPAKRSNMLSSFHETTTPTPTLRGPTKSKNSREGEDSSEKKKSNFGGKLKKLFGRSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH46</accession>
    <name>A0A1E5RH46_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphopyruvate hydratase</fullName>
            <ecNumber evidence="3">4.2.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1051</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(2R)-2-phosphoglycerate = phosphoenolpyruvate + H2O</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Mg(2+) is required for catalysis and for stabilizing the dimer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the enolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RH46"/>
    <dbReference type="FunCoup" id="A0A1E5RH46">
        <property type="interactions" value="1232"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH46"/>
    <dbReference type="InParanoid" id="A0A1E5RH46"/>
    <dbReference type="OrthoDB" id="1739814at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.10:FF:000001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.120:FF:000002">
        <property type="entry name" value="Enolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF1">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00178">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enolase N-terminal" evidence="12">
        <location>
            <begin position="3"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="11">
        <location>
            <begin position="145"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="347"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="374"/>
            <end position="377"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ86205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="438" mass="46893" checksum="49F5B068D7378D96" modified="2017-01-18" version="1">MSVEKVHARSVYDSRGNPTVEVEVTTKDGMFRSIVPSGASTGIHEALELRDGDKSKWLGKGVLKAVANVNDTIGPALIKANLDLGDQKSVDAFLNKLDGTANKSKLGANAILGVSMACSKAAAATKGIPLYEHLNQLIYGTTKTPLVLPVPFFNVLNGGSHAGGALAFQEFMIAPTGAPSFSEALRIGSEVYHHLKSLTKKKYGGSAGNVGDEGGVAPNVNTADEALDLIMDAIKEAGHEGKVGIAMDAASSEFYKDGKYDLDFKNPDSDKSKWLSGPELASLYNKLVDKYPIISLEDPFAEDDWDSWTHFFKNSKVPVVADDLVVTNPARIATAIEKKAADTLLLKINQIGSLSESIQAANDSWDAGWGIMVSHRSGETEDTFIADLVVALRCGQIKSGAPTRSERVAKYNQLLRIEEQLGDKAVFAGKNFHTANKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH69</accession>
    <name>A0A1E5RH69_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Lysophospholipase NTE1</fullName>
            <ecNumber evidence="3 12">3.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Intracellular phospholipase B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g1208</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the NTE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="3 12"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH69">
        <property type="interactions" value="123"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH69"/>
    <dbReference type="InParanoid" id="A0A1E5RH69"/>
    <dbReference type="OrthoDB" id="421051at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046470">
        <property type="term" value="P:phosphatidylcholine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001423">
        <property type="entry name" value="LysoPLipase_patatin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056556">
        <property type="entry name" value="NTE1_P-loop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF29">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE SWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24179">
        <property type="entry name" value="NTE_Ploop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01237">
        <property type="entry name" value="UPF0028"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 11 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 11 12" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="9 11 12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="14">
        <location>
            <begin position="669"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="14">
        <location>
            <begin position="769"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="15">
        <location>
            <begin position="1197"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="11">
        <location>
            <begin position="1201"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="11">
        <location>
            <begin position="1228"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="11">
        <location>
            <begin position="1349"/>
            <end position="1351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="141"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="1230"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="1349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ86224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1503" mass="168704" checksum="5E2D713D0533E78F" modified="2017-01-18" version="1">MNTSLTFVNTSTASMVSSLATTVALEPTAHSVQTVFQTQTITSVLYPTILSFPFVLTSVINSSIMFWGNLLAKTSWKLLGLVILKIPYYLIISSSIRVPIYGLLTIAFIAGKIAYGFIDKKYVHSYTEETKKLLQNSTYGTPTNTKTNSKNIKNELEGQESGENADLFDECKGLTKKSKFNKFFDESLEYNRNINGYGSKDKDYSSRNHTTHPRIFEPVNDESYLNRFLKAIQIFGYLESEEFNELTKSMKSERLSEGEILLLNDISGFVVLVEGSVGIYYKLDEKKDEENDEVAPAEKEDFQSSSHQFFPEDLNFDTAASDTESEEDSEDESVMDNMGFQSHCTVDGENFHLLNIVKTGNPVSSLVSILRLFTYDKNTESGPNSDVFSTTSVKLPQLIAKASSNCTIAIIPPESFQNLTKKYPRSASYMIQMILTKLYRVTFQTAHKYLGLTKEIMKTEITLNQNFEKNSQKSKQLHDFLINVIDNSTNSGNSLKGADNLELNSKSSITGVLGARQVTLAHSNKNSTTDLLATTSKNSSHYDLRNYKNSEMNSPFTPLAPIHSQEINDQKWRLSLIEIFFQYLGIDKTNIIPHDSSSTSPTEILSKSLDSITPQMPKIIHTNGLSIYKMPSSKNILKKKKSNTESTSTYKPEFSSSSESVDFDTATYEFSNALELEFIPKGSVLVNENENLDGLLYVISGTVQATCKDVDTGKDHKIYTVTSGNILGFLSSLVGNSNSLATLKAKTNLYIAFLPSKIIEHLCDKYFMIYLRIAETLIFSLSSNILKLDYALEWIHLGASELLFKKGDHADAIYVVLSGRLREFLNRDVIIKEFVQGESFGEVEVLTASKRMSTVISIRDTDLARIPRXLYEYLSLEHPSTMFKVSRLVAKQILMGKNEPSTQSKFVSENGFKYDFDLLIPPTTRDDESFGGNDRIESSTNYRTISILPLAPGLPVQDFAIKLVNAFKDVDRSVIAVDQRTVLAHLGKHAFDKLAKIKEAGYFADLEETYQTVIYIGDSPANSSWISTCVSQADCVLFLGDDQDLVARERLTDYEKILLRSKYTPRVELILLHPQRYVEPGSTQHWLKIRPFVESHHHVEFNIKRFMPVDSVINNNPSRPQVLSLSSIKNRFIEKVKKDKRFNTITKKTSKFIPNTLKTLIQQPFRRGDSSAPSVLHTHKNDFLRLARILSNQSIGVVLGGGGARGVSHIGVLKALEDQGIPIDVIGGTSIGAFVGGLYAKEYDIVPIYGRVKKFCSRIGSLWRMLSDLTLPITSYTTGHEFNKGVWQAFGNSRIEDFWLQFYCNSTNITESLHEIHTKGIAWRYIRASMSLVSLVPPMVDTNGSMLCDGGYIENVAVQELKRRGCKTIFAVDVGASDIRTKMDYGDYLSGFWILLNRWNPFSSHADVPSMTEIQLRLGYISSVNALEKAKLTPGVIYARPPVENIGTLAFSEFEETYQIGEKFGQAFLKDLHENNKLPKLPGSKLNIKGELPKPVLQRRNSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH71</accession>
    <name>A0A1E5RH71_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Lipid phosphate phosphatase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1606</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RH71"/>
    <dbReference type="STRING" id="56408.A0A1E5RH71"/>
    <dbReference type="InParanoid" id="A0A1E5RH71"/>
    <dbReference type="OrthoDB" id="10030083at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF155">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="7">
        <location>
            <begin position="141"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="301" mass="34382" checksum="DABC22FDF011A784" modified="2017-01-18" version="1">MLALLKTVTQIRVKNFPRWFQFNIVLCLAFAYFAYMEFSGELIPGTSIFTNRNYNLKRIIQITPAIDKPHTDNEFVSGIQCMLFVLCVPPLILLFTHYDDHILPTSTPAVSLSSSSPSFSSSSTSASSQSLSCYNVFNVINSYMLILMVNGLITNILKIIISNPRPDFLARCVPNFSYEQYSKLELEQCTRPLTDLLIIDGLRSTPSGHSSFITCAIVVLFQFLHSAYRPNFKWRNKLKLNFWCIVVVVVVVISRILDNRHHLQDTIFGIVIGGCVGHYGLKWFPRYYDVLKVKYKQELPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHE7</accession>
    <name>A0A1E5RHE7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PEX18/PEX21 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1081</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHE7"/>
    <dbReference type="FunCoup" id="A0A1E5RHE7">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHE7"/>
    <dbReference type="OrthoDB" id="4035272at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056940">
        <property type="entry name" value="PEX18_PEX21_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25098">
        <property type="entry name" value="PEX18_PEX21_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="PEX18/PEX21 C-terminal" evidence="7">
        <location>
            <begin position="243"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="50"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="329" mass="36478" checksum="C54A050C3C10AFF2" modified="2017-01-18" version="1">MFVSCSHNNNPLARLNNRVEQGAGSIRPQGGSNFHSKQPFQQQDIYNGNQLFSSSSHFPNQSKPQHSDQFPQHKLQEPGNSLQWLDQFQSMSVVDPTEASDQYKQFYAAFEKQQLGNVPTNVRATSPMERLSSPPMTMMARPSFASAGLASADSILVSSQNNTMEEQLANDTAEVAQDYSLVDQELEAMEAMQESSVSTSVSTSQIGVDQSATFLPEQETPLHASYQNETETHGTDLTFPDMEAQKFQNAATSVYKNLKDCKAFQRSGFLKLMRNVSDGDVTLQKSGNSVAYSQRNDTLASQEYVGLYSNSQKKTIGPDYFSIKDHTMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHQ1</accession>
    <name>A0A1E5RHQ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1029</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHQ1"/>
    <dbReference type="FunCoup" id="A0A1E5RHQ1">
        <property type="interactions" value="63"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHQ1"/>
    <dbReference type="OrthoDB" id="5330253at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018809">
        <property type="entry name" value="DUF2406"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28186">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28186:SF1">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10295">
        <property type="entry name" value="DUF2406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="249" mass="28049" checksum="DA352182D1BC8B8E" modified="2017-01-18" version="1">MGIFDRKKSDAGNKHKHTKSNSSGEAHAPDVKLTKDDVNHFKVRTASVHDPILSAVQEDQPFQEIGGNPNDPNARKSYLSMLNQGEMDPNEQYLNPDGTINTDAEGAENPRRSYHLTDVFGQPIVTPDISNPTRERDERPLDTIKSFEYAISGDPSWAQNLETQQYGFRVRPDFHLNFQQPQEISYDEEGNPIPQESPYGYNNNNPSGMMTEEQGVYVPSSSTPEVNLGKKKKKGLFGRKKKEKINVEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHQ5</accession>
    <name>A0A1E5RHQ5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">37S ribosomal protein MRP4, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1390</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHQ5"/>
    <dbReference type="FunCoup" id="A0A1E5RHQ5">
        <property type="interactions" value="758"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHQ5"/>
    <dbReference type="InParanoid" id="A0A1E5RHQ5"/>
    <dbReference type="OrthoDB" id="2320368at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000055">
        <property type="entry name" value="Mitochondrial ribosomal protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_B">
        <property type="entry name" value="Ribosomal_uS2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005706">
        <property type="entry name" value="Ribosomal_uS2_bac/mit/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_uS2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01011">
        <property type="entry name" value="rpsB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S2 PROKARYOTIC AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="20"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ86417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="432" mass="48082" checksum="4A8570CFCA93D36A" modified="2017-01-18" version="1">MSLPVSTKRVFPLVRLVGKRHSSTTATGTTHTNTSASVQSTGSVKTSENKPLKESIPKHIKAHQNEESLLPDTEFYALLRLRRFNEKVMQKLRSLQKDTQGSVNVSLEELSKQKLSPREKQLDEELDKFFEKHLHEKDLIKINNNRDEKTQQAATYFPTNVSSKQVLSKDFPNLNITDFGRPYTAQELFLRQLKHSATVGKLGAKIDNVYFPNNDIYNPLQANEVTIEHLMAAGVHLGQATSLWNTSTHPFVYGSYKGIHIIDLNKTLQSLKRSCKVVEGIAERGGIILFLGSEAQRRVISNAAKMCRGYGIHTRWIGGTLTNPTEIAGGWARQKVDMADNPTGEPLTDLQTDGLIKPDLIIVLNPKQNKTALKEAMKVRIPTIGIVDTDCEPSLVTYPIPGNDDSARSVNLIAGILARAGETGLNKRLRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI33</accession>
    <name>A0A1E5RI33_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">La</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1297</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RI33">
        <property type="interactions" value="752"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI33"/>
    <dbReference type="InParanoid" id="A0A1E5RI33"/>
    <dbReference type="OrthoDB" id="439993at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045180">
        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF140">
        <property type="entry name" value="ACHILLES, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="4">
        <location>
            <begin position="74"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ86547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="333" mass="38338" checksum="1B8CC2FBEB81AC6C" modified="2017-01-18" version="1">MSKPVLXKSNESASGVDDQSQTFKKPTLQAPLXNNEGANDNASDNAIDDDGSDEHEIDNKPQVPIRRSLSVGCREITEADYDSIVKQVEFYFSEHNLPYDVFMKNLIRESKNVDDPELSGWIPLTTILTFNRMKKWRPIDKVADILVSNSQKLQXSPNRDFIRPVNMEEIDFFMDKTRMKQFFDEQCSRSCVLNNFYLDEEKESGDAQEPVGVSNLQTKNLEQVESYLTXTFPQFDFKQIRLKYKHKRCGNNGTSKIFGNNVVLELNDEAKCTEFVDFVNQSEIMHNGYQLQCTSKRHHDITRASTKRVTKRFQGHQKITPKSLAKRRLSNSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI42</accession>
    <name>A0A1E5RI42_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Single-stranded DNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1515</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI42"/>
    <dbReference type="FunCoup" id="A0A1E5RI42">
        <property type="interactions" value="224"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI42"/>
    <dbReference type="InParanoid" id="A0A1E5RI42"/>
    <dbReference type="OrthoDB" id="1078367at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006264">
        <property type="term" value="P:mitochondrial DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04496">
        <property type="entry name" value="SSB_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000424">
        <property type="entry name" value="Primosome_PriB/ssb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011344">
        <property type="entry name" value="ssDNA-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00621">
        <property type="entry name" value="ssb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10302">
        <property type="entry name" value="SINGLE-STRANDED DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10302:SF0">
        <property type="entry name" value="SINGLE-STRANDED DNA-BINDING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00436">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002070">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50935">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR002070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ86589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="121" mass="13741" checksum="39CE707B156022D1" modified="2017-01-18" version="1">MLFKRAFSQTSKKLDFAKMSIIGRIGNDLVSQQGAKGTPFLKYNIAVQPQKDAETNWFNVVCFSEQQIKFMEEYVRKGALVYVEANVRTSNFEKEDGSKSYGVSFIQKNINVLQNGKEVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIB8</accession>
    <name>A0A1E5RIB8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">SWI/SNF complex subunit SWI3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1506</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SMARCC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RIB8"/>
    <dbReference type="FunCoup" id="A0A1E5RIB8">
        <property type="interactions" value="1088"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RIB8"/>
    <dbReference type="InParanoid" id="A0A1E5RIB8"/>
    <dbReference type="OrthoDB" id="118550at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048858">
        <property type="term" value="P:cell projection morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000020">
        <property type="entry name" value="SWI/SNF complex subunit SMARCC2 isoform c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032451">
        <property type="entry name" value="SMARCC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15381:SF1">
        <property type="entry name" value="CHONDROITIN SULFATE PROTEOGLYCAN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15381">
        <property type="entry name" value="CHONDROITIN SULFATE PROTEOGLYCAN 5 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16495">
        <property type="entry name" value="SWIRM-assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SWIRM" evidence="9">
        <location>
            <begin position="554"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="749"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="10">
        <location>
            <begin position="788"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="463"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="698"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="990"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="108"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="155"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="201"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="220"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="241"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="259"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="292"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="386"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="409"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="426"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="479"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ86624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1031" mass="114949" checksum="485399B46B37F90B" modified="2017-01-18" version="1">MEGFMEFGESADGGGNDQFDFGGNDQMENLIGTEVEGDDLNNQIFQQDQGVHDSDLFGESNSNENNNEVEDPEAEQVYEKENDEEENENNLKDLEGNEEGEAEKKENDEVEEDKNENENVEVEEEEQSSLFGENEDNDNSVYAQMDGAEELADENNVKEIKSIEQENDAKNDASQLREEPMQKRSASQEVEESTETQDSAKNPDFDKIEESKGKNGDTPLASNADTATAPQSEEQGAESVSVGNNTNDLNATTSNGESFKQEPDAKAAHDESSQNISSSGIEQPAVTEASALDQSPATQTDATKDYNGNLQNENINADENPEKIVIAEQKSNLQTAAGDALQEEENLDPAKSMGAAIETTSSLNDEEMQNASAVEDAQPTREKQSTEQTQLQNNESLPVSADQEIGNNDESKQDIKNDIDSNTNHEILENNVEHNESQSEPNNEPTIEGKAEKESLDQIHGENLHDIVNPPVSGQTDIDESKENVPNDDQAKDVDMDNGDLETGNYGANAGANHAASSIPIKISNSESSLKSVDMQENEISEPIDPSIPQTHDVVIPSYSCWFDLTKIHSIEKENLPEFFTNRIVSKTPQIYIKYRNFMVNTYRISPDEYFTFTSARRNLCGDSGCIFRIHKFLNRWGLINYQVDAQKKPANIIEPPSTKDFEVKHDAPRGLFPFQSYKPSVQLPDLIRLKKLMNQELQKSNSVDPEQPPLKKRKTSLQMGDVKAETHSQYETNTNAKDNAPDAVVISNDSAETDGWSAEEETKLVKLISDQTLSASKPLTSDIWVTASNLLGNKTPQECILKFLQLPLEDPFLMGQENGSQNGIGPLKYAPYLPFSKADNPVLSTIAFLVGLCDPSEVKKMVQHITKDYDTFNDNNKPTLKDGVHFGLSALGIRARHFADNGEKELLDVTNRLVHAQMQKVDLKLEKLKVLEKSLTLKEHYFLQERQKFIFEKLQFYKEYENLVSKLDDGTKQKPDLRKHLDILAPNLLKPSAAKDATKEKQESGEDEENIEDSLPISVSAPQFYRYWSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RMY6</accession>
    <name>A0A1E5RMY6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g630</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the importin alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RMY6"/>
    <dbReference type="FunCoup" id="A0A1E5RMY6">
        <property type="interactions" value="1203"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RMY6"/>
    <dbReference type="InParanoid" id="A0A1E5RMY6"/>
    <dbReference type="OrthoDB" id="29145at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.690:FF:000003">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000021">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.690">
        <property type="entry name" value="Importin-alpha, importin-beta-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032413">
        <property type="entry name" value="Arm_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002652">
        <property type="entry name" value="Importin-a_IBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036975">
        <property type="entry name" value="Importin-a_IBB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024931">
        <property type="entry name" value="Importin_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16186">
        <property type="entry name" value="Arm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01749">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005673">
        <property type="entry name" value="Importin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51214">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="IBB" evidence="9">
        <location>
            <begin position="2"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="143"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="186"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="354"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="542"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ88251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="561" mass="62100" checksum="9F8BEEAD4913F44D" modified="2017-01-18" version="1">MDPSDSNTSKNKFVPEYRRTNYKNKGRFTADELRRRRDTQQVELRKAKRDEVLLAKRRNVNNNSSAAAAAAAALDSDSDDEFTSAQKDSENNEFLKQLQQELPTMIQQINSDDVNAQLSATIQFRQILSREQNPPIDLAINAGIIPRLISFMQENQPEMLQLEAAWALTNIASGTSEQTKVVVDAGAVPSFIQLLLSPSVEVKEQVIWALGNVAGDSPKYRDYVLECGAMQPILSLFALNKTSLIRTATWTLSNLCRGRKPQPDWNIVSQALPILSRLIYSLDTETLVDACWSISYLSDGPTQAIQAVIENRIPKRLVELLLNQSTTIQTPALRAIGNIVTGNDLQTQTVVNSGVLPALAKLLYSPKENIKKEACWTISNITAGNTNQIEAVIAANLIPPLIQLLSEAEYKTKKEACWAISNASAGGLEKPEIIRYLVAQGCIKPLCDLLEVADSKIKEVTLDALENILKVGELEKEAKGLAINAHAEYIDQAGGTDKIFMCQQDANETIYEKAYNIIDKYFHDDDEEDVVDETLNNNASQQGNTFSFGTNANANQSFNFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN87</accession>
    <name>A0A1E5RN87_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g954</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN87"/>
    <dbReference type="STRING" id="56408.A0A1E5RN87"/>
    <dbReference type="InParanoid" id="A0A1E5RN87"/>
    <dbReference type="OrthoDB" id="10261433at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43094">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43094:SF1">
        <property type="entry name" value="AMINOTRANSFERASE CLASS-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ88345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="472" mass="51855" checksum="B7174B658D1274DA" modified="2017-01-18" version="1">MSTDSYVFQSVINKKPPHVLGGKGVRMTIEKDGVVYDDIVDGVTGAAVGALGWGDEDVLKIINDAAKNSSYTYPSLIGNQASEDLAKFYIDHSPKGAFASALWCCSGSESNENALKIIRQYWLERGKPKKTKFISRETSYHGFTLGALSISSNGRAELFKEVLIDQENTCLKMPVCFPYRFQKEGQTTEEYVQELMDALEEIILKNDPETIASVTVETLPGSSLGTAPPPPGYLPGIRRLCDKYDILMHLDEVMCGTGRANPNGRLNCWENFLEPGQFPDIQTVGKTLGSGYVTIAGILVGPKIKDAYVKGSGQIIGSHTYASHGFNCAVALGIQKKIMENGLTKNMFEKGNLMGEKLKEALLLGKDCENNIVGDVRGIGGFWSIEFVKNKKTKEPFAKKLDVGHKMQDFCFKNKINVMGMQGSCSYKTGEGDFILLAPSFIITEEDVDEIVKRTVQAVKDCTEWLKEEGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNA5</accession>
    <name>A0A1E5RNA5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g988</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNA5"/>
    <dbReference type="InParanoid" id="A0A1E5RNA5"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ88369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="500" mass="56532" checksum="D6AEA9E8E8C86467" modified="2017-01-18" version="1">MKRTMQLLETSGLAKNYTKSLSVSKAAATATSEVERVNELQNTIRYLTNQNKATSILLGGSDIWQGHSNDLDNNSSKAKKRNELPVDTLVSKMNETLPNIETLKKNTIDSHYNSIVDLLNVATGNANGFSSSFTSMKKNLQNDSALLAFQPKSGKSSGYDLFVKFFTTYKESQSKEVNIQAVSQFLSSSSNFVTIDDYYKMVKTIKYLLNSKLIATENKNKLETEIVIYCYYMIFRKTSQKQLSLIDRDIFNSLVTFLFETRDGNLLFNSLHLHIKRLLFVLVKSFQPLKAAPDMGLETFFSKIKTLLDETTTTNKNSSFFKDGTAKTIVLMFQVFSEEAIELKNLLEPYIGNGEFNSNNRTQWTAFKAEANSVFSNEQRLFIDVLCLINTIKRELLLQSTNKHEKELPHLVQIEGIMKTINKSSIQNKILNKTAANGDKFYYDIAKVLEALQPHIDIIHASTAFSPVKVNEIGHQVNSLKLQVAHTVKKHKVDFILGIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNC0</accession>
    <name>A0A1E5RNC0_9ASCO</name>
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        <submittedName>
            <fullName evidence="2">Nucleoporin NSP1</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">AWRI3579_g690</name>
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    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
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            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="56408.A0A1E5RNC0"/>
    <dbReference type="InParanoid" id="A0A1E5RNC0"/>
    <dbReference type="OrthoDB" id="4095576at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
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        <location>
            <begin position="36"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="286"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="690"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="822"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="211"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="301"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="360"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <begin position="378"/>
            <end position="391"/>
        </location>
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            <begin position="398"/>
            <end position="408"/>
        </location>
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    </feature>
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            <begin position="454"/>
            <end position="466"/>
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    </feature>
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            <begin position="835"/>
            <end position="844"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="OEJ88382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1043" mass="110859" checksum="766F0250A8DF86DF" modified="2017-01-18" version="1">MTESSCISKPAPPKRSISSTLSSLWKGDKKTVVAKTALTQPEIQDLDEALENNTKDSNANDNTKRPALLIVDPKQRLMLLKYKQMVRQGKLEKVLQLLEDKSQKVVLNNSWSTKGLDTTEKTKENESSKEYKFTLPINSASHKKYKPTTTTTRKNLGFRFDPHEYEYDMSEYDTISNTNGYSSHNFAGNVDSIAQTTTNETGKIQGKRKLSNSTTKSSFSMGSSTPVSYVQKSNGIITSDNDKGLTDNQLKLLKGTFPQQSSSNDDAVKERDISIVKIAPISEEDLDEEAENTTFPHILSKQDNNNGNTNNKKHIKNLQNPQSAENEAIKPTAAFNFVKKPESDNLIKIQPISNEQFKPSSENSSSLFSKNQENPKISSSTSSSFNFSLGSKKNEANLPDENDKDGDDSERPRKKRTPQFSFDSAKTSTFQSASESASKPASKPTFSFGSGPSTEKEQAQPDKQDKPVFSFGSGAKDKPTSDAATNDKPSFSFGSSSTGKSTEDSKTPKFSFGTAGLAEKKEEATAKPTFSFGSNNAEKNDNTPKPAFSFGAATTGKTEEANKTALITSGDSTQKKEEASKPVFSFGTDATQTNNVPAKPTFSFGAGATEKKEEPVKPAFTFSTGTTEKKDESAKPAFSFGVGESNNNDKAATPSFSFGAAGSEKKDDVVKPSFSFGAKTAETKIDATKPSFAFGSSSAANKNTEITQPSFSFTGANAEKKEDPAKPSLSFGLPSTTNKDEGTKPSFNFGAAKPALSSGPGSLFNGSTPSSAPSTASGTLKFDFKNFNKPALAPPTSSLAPNIGNATSVSFNPGASTDFSLGNLNKRVRDGSGAPAQSTSSFSFGNAAPAQPQPVPVFKPSSTINLNFGGNTSGSSGSALPPSAIFGAPNNNNNSSGMNTGMSGNSFGSPMPNNFQPNAMQPNGFQNNAFGKHAHSPSPANIFGGSSSTPPPMGMNMGMNGQGMFGGMNNGMNNGMNNGMMNGMMNGMNNGMNNGMNNGMNNGMNNGMNNGMNNGMNMGMNPAMGGQMNLPPGRKLARPRRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNC1</accession>
    <name>A0A1E5RNC1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">[PSI+] induction protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g696</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
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            <taxon>Saccharomycetes</taxon>
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            <taxon>Saccharomycodaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="FunCoup" id="A0A1E5RNC1">
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    <dbReference type="STRING" id="56408.A0A1E5RNC1"/>
    <dbReference type="InParanoid" id="A0A1E5RNC1"/>
    <dbReference type="OrthoDB" id="3980401at2759"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="PSI_induc_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40018">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR40018:SF1">
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            <begin position="49"/>
            <end position="73"/>
        </location>
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    </feature>
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            <end position="246"/>
        </location>
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            <end position="172"/>
        </location>
    </feature>
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            <end position="224"/>
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            <begin position="236"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="246" mass="28519" checksum="AC23DD67E10FDDBD" modified="2017-01-18" version="1">MFVPNNSEPTEELCIQQSILKIRAWSDVTSTAKAFKHWNTCMENKACKIIVIVLICLGGILAIWLLGSLLSMFKTGVTGICDFLCWCCPNRNRNRYPDGDFQRQSHYQMQQQAPVPVIQQPVYVQAPNPAMYNGRKDLHSYYEEFGSSKEDGLQIEEQAFDLNAHKNQDRNTKGKKQTPVAEEFYTPSYLAGDKSNNNDYSHDYNKFANSNTDFETTNQQTQYNPYDRNQYPSYEQMKKSRKDYYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNC9</accession>
    <name>A0A1E5RNC9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Topoisomerase I damage affected protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g655</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ascomycota</taxon>
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            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="InParanoid" id="A0A1E5RNC9"/>
    <dbReference type="Proteomes" id="UP000095728">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="384"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
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        <location>
            <begin position="94"/>
            <end position="156"/>
        </location>
    </feature>
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            <begin position="546"/>
            <end position="573"/>
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    </feature>
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        <location>
            <begin position="597"/>
            <end position="628"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
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        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
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        <location>
            <begin position="36"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="101"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="598"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="628" mass="67340" checksum="BC8877965BFB94D3" modified="2017-01-18" version="1">MIAQGQVRNFNSTSVPTTSISSISSRSSKRTPSISETTIDTSNSLSTSNFFDTSSVPKSSTALDTGKASTSNIPDNGLSITSVSTNIESLSSNNITSTHLPSSITNPTSASSFSSDSRTRTQSSLTQSSLAQTSTFSSLQTSNNGSPSSDVSPATSVLSVSTSPLSSSFFETSNFSTPITTSIWHPSSISETSSKYQTSSRLASFGFTDTRSTASNISSGSTLTSSNGNQIFSSFTGLNIVTTTIKSSKNAITSSTSASDTSFVTTTSQNLWTETQFVVATSVVTINVDSNGNTYATPADYQSASSTSMWTTGDYSKSVTDYVQVYQITDTSTTFECSVTTTKKPKHSTITTDSSYYNAHLKSLLSGQSSGSSSTNSHGNSHKILVNSLVGGIVGGVAGVFFILLMGYYAYKWLNRRQKVTALSCYLAGDERVNNNNNNNNESGFQHGRGRKVNIYQEREMMDDYDANAPPPGIFSRQKENIKNFAKSAFTGTGKKSDKNANPFNEEFDFRNRNFVMPQAKSSGQKQEMQPPIIVETTAAAILRGSDHDNSFSSDDFEDSTSPNGTVGMHDIGQYYGGSTQFDPFADQHPFAHEAFSSDESFSDMSTDITSLSPNSRPPSQSIFTERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNF8</accession>
    <name>A0A1E5RNF8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein ECM3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g684</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNF8"/>
    <dbReference type="FunCoup" id="A0A1E5RNF8">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNF8"/>
    <dbReference type="InParanoid" id="A0A1E5RNF8"/>
    <dbReference type="OrthoDB" id="435607at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040254">
        <property type="entry name" value="Ecm3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_transp_PIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31274">
        <property type="entry name" value="PROTEIN ECM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31274:SF3">
        <property type="entry name" value="PROTEIN ECM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="447"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="489"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="521"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="560"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="600"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="180"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="222"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="627" mass="69630" checksum="C824DDFC4E0E997B" modified="2017-01-18" version="1">MSAELVPLGQVIYSCVKPIFKIYLIMGTGFLLAKLDILNGEAIKMISQVVLTVLLPCLSFNKIVANIEDKFIKDVGIIVLSSVLLYGTGFLFGWLVRIFMPCPKKWYGGIICASMFPNISDLPIAYLQTMNNNIIFTQEETDRGTAMVIIFLATFLFSLFNLGGFQLVEIDFKDDLPQKNKDEETEIESVEKPSPSPLPATSSSDTAGTKNSKYTKTDENEGNNTDMKSNHSNSADLASLDSNYYSTSNLPSTNQPQLYTNHEDGTNLSTETVNHPHSHTQDDNVSATNSTRSRRRSLVGSFLSRADSMQSYKNNSVHSNKDGASSIAGNNKLIRQYSRVDTMGNPVPEDSMARYLSLQALQQEHGTEEHEPGQESVHSMNTGFTTAHDNKSLLNRVKSSKLAKMVTNDVGMHKSDIDDSVKAFPKKFHFMKYAVFFMQNCLRPCSIAVILGLVFAFIPWVRGLFVTTSNAHIPNAPDGEPPLSFIMDFTSYVGAASVPFGLLLLGATLGNLEIRALYPGFWKSAVVLVILRQVVMPIFGVLWCERMMKAGWLDKTTDKMVLFVIAINWALPTMTTIIYLTAAYTPIDATETPQMDCASFFLLLQYPFLVISLPFLATYFIKVQLSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNI9</accession>
    <name>A0A1E5RNI9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Cytochrome c oxidase polypeptide 5A, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g824</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome c oxidase IV family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNI9"/>
    <dbReference type="FunCoup" id="A0A1E5RNI9">
        <property type="interactions" value="121"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNI9"/>
    <dbReference type="InParanoid" id="A0A1E5RNI9"/>
    <dbReference type="OrthoDB" id="186013at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.442.10:FF:000002">
        <property type="entry name" value="Cytochrome c oxidase subunit V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.442.10">
        <property type="entry name" value="Cytochrome c oxidase subunit IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004203">
        <property type="entry name" value="Cyt_c_oxidase_su4_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036639">
        <property type="entry name" value="Cyt_c_oxidase_su4_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10707:SF10">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10707">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02936">
        <property type="entry name" value="COX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81406">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="91"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ88457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="150" mass="16805" checksum="DE94AB6A9B335997" modified="2017-01-18" version="1">MLRAGLLNKSITRCASTSATTTLSKATIVDLPLRWESMPSTEQENIVSKLQERQKLPWTELSDIEKKAVWYISYGEWGPRRPVHAKGDASFIAKGVVVGLLGSLGLFGFVRAFAQEPPKTMTKEWQLQSDEYLKSKNANPWSGYSQVQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNL3</accession>
    <name>A0A1E5RNL3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase</fullName>
            <ecNumber evidence="3 12">2.4.1.131</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g833</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes, on the cytoplasmic face of the endoplasmic reticulum, the addition of the fourth and fifth mannose residues to the dolichol-linked oligosaccharide chain, to produce Man(5)GlcNAc(2)-PP-dolichol core oligosaccharide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>an alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + 2 GDP-alpha-D-mannose = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + 2 GDP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:29523"/>
            <dbReference type="Rhea" id="RHEA-COMP:19515"/>
            <dbReference type="Rhea" id="RHEA-COMP:19516"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:132511"/>
            <dbReference type="ChEBI" id="CHEBI:132515"/>
            <dbReference type="EC" id="2.4.1.131"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11 12">
            <dbReference type="Rhea" id="RHEA:29524"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.131" evidence="3 12"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNL3"/>
    <dbReference type="FunCoup" id="A0A1E5RNL3">
        <property type="interactions" value="412"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNL3"/>
    <dbReference type="InParanoid" id="A0A1E5RNL3"/>
    <dbReference type="OrthoDB" id="2276068at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004377">
        <property type="term" value="F:GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03806">
        <property type="entry name" value="GT4_ALG11-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038013">
        <property type="entry name" value="ALG11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031814">
        <property type="entry name" value="ALG11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45919">
        <property type="entry name" value="GDP-MAN:MAN(3)GLCNAC(2)-PP-DOL ALPHA-1,2-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45919:SF1">
        <property type="entry name" value="GDP-MAN:MAN(3)GLCNAC(2)-PP-DOL ALPHA-1,2-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15924">
        <property type="entry name" value="ALG11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 12 15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="ALG11 mannosyltransferase N-terminal" evidence="14">
        <location>
            <begin position="159"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="13">
        <location>
            <begin position="384"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF15924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ88430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="608" mass="69957" checksum="B0936B8F0DE144C9" modified="2017-01-18" version="1">MSDIFKPAQSSAVTAVLKDSVVYRLFGEITTARFLVFLAIALIVLVREYPKLLKQIFYKPPKDYLKRYMAHIVKFEELAKKNDTSSKDKTVMINFGHKSGSVRRQFILNTVSPSAINYSNYLSLTKFKNNDKIALSKEDRDYYFEMIKNIQQVDCENKYIIGIFHPYCNAGGGGEKVLWKLVADLQAQLPLSVKIAIYTGDLDIAPADIINKVNLKFGYNINISNITFIYLTKRKYVDGKYWKHFTLIAQALSSIMLGLEAVRKLPPDCFIDTMGYPFVYPVIKHFLGIPVVSYTHYPIIQQDMLNTVQKSFVKKVYWRALMTWYKFVGHSVDISITNSTWTNNHMNEIWSSKTNTIIYPPCFDASQICVDNEISTLEAYKSFKKFQCVLVAQFRPEKRHRLAISEFSQYLKSDSTEKGGNPLKLIFIGSTRTSEDDDYVVSLKKYAFEELNIPHDLLEFQCNLSFADLQKKLQESMFGLNCMWNEHFGISVVEYMASGCFPIVHASAGPLLDIVVNNYSNGVFFTDKSDPDYAKSDYLSLNKAFEYVSQTEKLKEFHDIALKNIEIVKNKFSDEKFDQDFNTKVLSEMKRLVTRSKTARLEDDEETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNR0</accession>
    <name>A0A1E5RNR0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADPH-dependent 1-acyldihydroxyacetone phosphate reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g804</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNR0"/>
    <dbReference type="FunCoup" id="A0A1E5RNR0">
        <property type="interactions" value="252"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNR0"/>
    <dbReference type="InParanoid" id="A0A1E5RNR0"/>
    <dbReference type="OrthoDB" id="2102561at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000140">
        <property type="term" value="F:acylglycerone-phosphate reductase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169">
        <property type="entry name" value="NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169:SF6">
        <property type="entry name" value="NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ88527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="316" mass="35244" checksum="2FB3735444814500" modified="2017-01-18" version="1">MQTPTRTKVALITGASSGIGYELTQQLVSKLHYQVYAAARRVGPLEELQAKLGADKVIPVQLDVCDSSSIEKLKQKLMEELPERKLDLLYNNAGQSCTFPALDVTSDNIEAVFRVNVFGPMNLTRELSPFLINAATKGNVPVIAFTGSLAGTSPFPFASVYAATKAAVHQYARVLHLEMKPFGVKVLNVITGGVHTDIADKRSLPATSIYNFKEGLEAFKYRQEMSKNNHPEEPKDYCSEIIKDIAALQNSSRDPVDVYRGTKALFMRIVMMLVPYYVLEMAFMRKFKLNPAFNAVKARKREDAAEEKIEEKEKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNU7</accession>
    <name>A0A1E5RNU7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g900</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNU7"/>
    <dbReference type="FunCoup" id="A0A1E5RNU7">
        <property type="interactions" value="425"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNU7"/>
    <dbReference type="InParanoid" id="A0A1E5RNU7"/>
    <dbReference type="OrthoDB" id="3972509at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044611">
        <property type="entry name" value="E3A/B/C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700:SF2">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="7">
        <location>
            <begin position="605"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="5">
        <location>
            <position position="916"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ88551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="948" mass="109793" checksum="8823A55F849159EA" modified="2017-01-18" version="1">MNNFTGTAKRRNVNLSTTDRRSRRDLIDKASRERERRAAERKEFVAAVVITKNIRRFLTLKRLYEQMLLQYRNHTFDFQVYQAQTLISIFYPSFLEFDLQNDFLLTGVLLENFGAQTSNLKLLNRKLLLSLNELSNLHLSSTQPYVSKIWSLINTTGNDKEYVVGQVIKYLENIAPYQLDDPNMLLCLREFLKADDFTKALLKHDFVLKTNILLYPVFQKLEIPTDFFILNKTFKLNKMQEVVNIIINLSFFANLQKIEPYYFDFVVQTMVTNEDLILANDSWTILKKYNSDVFPIYDYFCNNLIDEYMSKDTHSLAPISLIFKICTPSAYRKSIISVLVQGKLLQEVYKNVVESNQLRYLELLIDMSNVYLFTKTDFELLGNRSSAGMKTNQENTLSLNQLLKLTICLKEILFKSLWSQVDDITSPNISQQTIEKLLKLLKMLQVRDNRAKLFKDPNVWTIQNEEFDNVNITNLFMQFEEYYREISDSIEEETAFDDVLKTAKLRDQSTEIFDRFIERKKLMRQASKRQIQKFQIMGKFPSFISFEERVNWFQNVLIATEKSKLGVNNDEFLLPWLMRNNMPRIEATISRDNVLLDSFNAFDSLGEKFKGKLSLTFTNEFGEEAGIDGGGLTKEFLTSVTANGFLEHPALFAENIDHEIFCKPYNNISLENYRLIRFMGKCLGKCLYENVLIDVQFAPFFLNKLLKCDRFAGGYKSSFDELSSMDSVLYKNLMQLWTMSAKELESMGLSFEITDDENPYGSMIELVPNGAQIIVDRTNVLDYMNKVADYKMNSKRMLASMKAFIEGFNTIIPQHWIGLFTPLELQKLISGGESHINISDLRNHTEYGGYLPTDETIVDLWDVLENELSDEEKAKFVKFVTSVPKAPLSGFKALQPLFGIRNAGRELGKLPTSSTCVNLLKLPDYRDKKLLAAKLRKSILSESGFDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP01</accession>
    <name>A0A1E5RP01_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">U4/U6.U5 small nuclear ribonucleoprotein component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g936</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP01"/>
    <dbReference type="FunCoup" id="A0A1E5RP01">
        <property type="interactions" value="382"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP01"/>
    <dbReference type="InParanoid" id="A0A1E5RP01"/>
    <dbReference type="OrthoDB" id="30343at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040107">
        <property type="entry name" value="Snu23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45986">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45986:SF1">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="164"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="170"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="190"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="223" mass="25992" checksum="7A5AE87631578AF6" modified="2017-01-18" version="1">MSSEKSNFGRRTWDRDEYLQLKKSNQLQTSFASKLNTLQTDNKQLYDKLYQKYGTQNYDTLLATQLKDVNKLMLKSSNDDGSTARKGKKFGFYCELCDFTFNNNMQFVSHLNEKVHFDSFEKVFGQEYVRDKRDNDDIPLSEFNQTVSSLLKIYQKELKTKLKKSLPTKNASSSSGVQNQVAKPEDKDKLKRSKLKKNKKKQKKQPNATKDDIAKIMGFNKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP52</accession>
    <name>A0A1E5RP52_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosome biogenesis protein UTP30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g980</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP52"/>
    <dbReference type="FunCoup" id="A0A1E5RP52">
        <property type="interactions" value="391"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP52"/>
    <dbReference type="InParanoid" id="A0A1E5RP52"/>
    <dbReference type="OrthoDB" id="3972474at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ88664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="39903" checksum="1A23A6557A1F0473" modified="2017-01-18" version="1">MSQASKALASLLQHYNTDPELANDKTINLIVNFQSATDIKSPIRNVSIHKFKNVPIVVSIPNKINKKIRNVEILMITKNPSTFYIEKLRECDALLPKTSIELEKELENKRVYAKSSWRDSPNLKSNIKNLGPKLRTAQEGKAVISNTKDLQATKSQFVDNSDSSELFSKIMSVKQLKSMSKSKEFNNLLKTHEYIIADQRVVPEITKLFPQKSRKQPFMIRLASADSKKRDIEKKKKFAKKHNHLNKMEKAELDSMDLKFVFSQVKSITKNTSCVLPLIATTINKYDKSLKEKSVVDLNFIIGKPNVHSVEEMVQNIEAIVKYINENLLKSGSQIKGMTVKTDNSQSLPIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV48</accession>
    <name>A0A1E5RV48_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA replication licensing factor MCM2</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">DNA replication licensing factor mcm2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Minichromosome maintenance protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">AWRI3579_g476</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RV48">
        <property type="interactions" value="1024"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV48"/>
    <dbReference type="InParanoid" id="A0A1E5RV48"/>
    <dbReference type="OrthoDB" id="3971837at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006267">
        <property type="term" value="P:pre-replicative complex assembly involved in nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17753">
        <property type="entry name" value="MCM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000002">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1640.10:FF:000003">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000138">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008045">
        <property type="entry name" value="MCM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF44">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12619">
        <property type="entry name" value="MCM2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01658">
        <property type="entry name" value="MCMPROTEIN2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="20">
        <location>
            <begin position="682"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="266"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="618"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="23"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="97"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="274"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="630"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00074927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00078186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OEJ90811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1048" mass="118134" checksum="68FA84A88E4FAB40" modified="2017-01-18" version="1">MSSSPPPPIPPSSFHSNDEDENNNNFLSNSQNFNNSAGGNRRRRRREITFESDSDEDNDDDDDDDDDDDIVQVPGRLQSVSLHAHLPSSPQAHDLNDLDDNDEDDEDEDLTRNRNILPSSPGGGGPRIRARRTQGDXLLDMGTPQPVAEDDLEQDVYEYGDVPELQEEGQFVDEDGVAENNVXGDXFAHHQGQGLLEGDDLEDLEEQMNEVDLMGEGMERDYVARDREDQYDLQDSATIDNTRQRELDIAARRRIDQQLNQRDRLLSNRQEAYLGDDDADEDGEGETQLDQYGMPIQKKRRRRHQYETQDDEDLLMSDLEDGEGXDPLQQELTLETLSDVRAPSFSEWITQPIVSRTIARELKSFLLEYTDEYGRSVYGSRIRTLGELNSESLDVNYRHLSESKAILAIFLAKCPEEMLKIFDTVAMEATKLHYPDYARIHSEIHVRITDYPAINNLRQLRETHLNSLIRVTGVVTRRTGVFPQLKYVKFNCLKCNALLGPYFQDSNEEVRISYCTNCRSKGPFRTNMEKTIYRNYQRLTLQEAPGTVPAGRLPRHREVILLWDLVDIAKPGEEVDIIGIYKNTYDGQLNARTGFPVFATVIEANSIKRRDGLTAALESKTRKTGTTQGNDDDDDDDDAENSQYSSSQADEDAAMSGGDIDMFNWTEEDEKEFRKLSRERGIIDKIISSIAPSIYGHRDIKTAVACSLFSGVSKNVNGKHSIRGDINVLLLGDPGTAKSQILKYVEKTSHRAIFATGQGASAVGLTASVRKDPITKEWTLEGGALVLADKGVCLIDEFDKMNDQDRVSIHEAMEQQSISISKAGIVTSLQARCSIIAAANPVGGRYNSTLPLSQNVNLTEPILSRFDILCVVRDLVDEETDERLANFVIDSHIRSHPESKEKPKTMSNTADVVDAEVEANKVRSQAEKENEISPIPQKLLIKYIYYARTKCQPKLLNLDQDKVGRVYAALRRESITTGSFPITVRHLESILRIAESFAKMRLSEFVSSYDLDRAIKVTVDSFVGAQKISVRRQLQRSFAIYNIDSRSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVJ9</accession>
    <name>A0A1E5RVJ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="10">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g489</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="10">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="10"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVJ9">
        <property type="interactions" value="674"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVJ9"/>
    <dbReference type="InParanoid" id="A0A1E5RVJ9"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000040">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000073">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="12"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ90972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="367" mass="42363" checksum="2D4BB9B666E54E55" modified="2017-01-18" version="1">MARIIFNISGDFQIXSLIGQGAYGVVCSAIHKPSGETVAIKKIEPFEKPLFALRTLREIKILKHFEHENIISIYNIQKPLNFSEFNEVYIIQEYMQTDLHKVISTQDLSDDHVQYFIYXILRGLKAIHGSNIIHRDLKPSNLLINSNCDLKICDFGLARFDEEDNANNASNTNNKNSIIDKMLMTEYVATRWYRAPEVMLTASKYSKSIDIWSCGCILAELLLKRPIFPGKDYKSQLILIFQFLGTPPVEELMKLPNKRARQYILQDLPHYSLEMCGLNPTNLVKIFGTNCNPXAIDLLLKMLKFDPSKRLTVSECLMHPYLSNYHDPKDEPNGTVISKEIFKFDYYKDQLSTKDLKKLLWNEIFDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RW07</accession>
    <name>A0A1E5RW07_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Ribosomal RNA-processing protein 8</fullName>
            <ecNumber evidence="9">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g434</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N(1) position of adenine in helix 25.1 in 25S rRNA. Required both for ribosomal 40S and 60S subunits biogenesis. Required for efficient pre-rRNA cleavage at site A2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
            <location evidence="1 9">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the methyltransferase superfamily. RRP8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="9"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RW07">
        <property type="interactions" value="313"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RW07"/>
    <dbReference type="InParanoid" id="A0A1E5RW07"/>
    <dbReference type="OrthoDB" id="10258825at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016433">
        <property type="term" value="F:rRNA (adenine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2150:FF:000001">
        <property type="entry name" value="Ribosomal RNA-processing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000320">
        <property type="entry name" value="Ribosomal RNA-processing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2150">
        <property type="entry name" value="Ribosomal RNA-processing protein 8, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007823">
        <property type="entry name" value="RRP8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042036">
        <property type="entry name" value="RRP8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12787">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12787:SF0">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05148">
        <property type="entry name" value="Methyltransf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 9 11" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00076672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ91019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="399" mass="45792" checksum="C0A29CED9C9589D0" modified="2017-01-18" version="1">MSLFKVDGWDIKSSKVAADRADKKSRKSKDSKRQKNFKXDNKTLPSENEQSQEENESSFETSKLKTQAPKTEKKVLKNTVKKGDKKAPAKLVENKGEKTETIPSSFTPNTNKKLTPLQQKMMAKLSGSRFRWINEQLYTTTSEKALDMITKQPELFHEYHQGFRSQVESWPENPVDVFVNSVIKRGASRPINAPGGLPGIVNPDTNQKKVVIADMGCGEAQLSLDVSNYFNKYNKKNKQKKLVEVHSFDLTKYNDRITVADVKHVPMEDESCSIVVFCLALMGTNFLDFIKEAYRIMAPRGELWIAEIKSRFGDGKGDDFVEALKLQGFFHKSTDNDNKMFTKFEFHKPSEDILKERIAKLERTSKFVEVQSKKEELDSKREKKAEGEWLLKPCIYKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RYS4</accession>
    <name>A0A1E5RYS4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein TMA23</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000002">
        <property type="protein sequence ID" value="OEJ92150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RYS4"/>
    <dbReference type="InParanoid" id="A0A1E5RYS4"/>
    <dbReference type="OrthoDB" id="3366546at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050656">
        <property type="entry name" value="PINX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149">
        <property type="entry name" value="G PATCH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149:SF26">
        <property type="entry name" value="PROTEIN TMA23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="245" mass="28993" checksum="C4B8B43526613EAB" modified="2017-01-18" version="1">MNSREYLQSYGWKEGEALQKGGLKKPILVKHKKDKKGLGNLHNHDDGDAWWERLFDGQLKNIETVVAPVSGKSKGTHKIHFKQKNEAPVASGVDKRNSPLYRWFVKGEGLKGTLGETEQELVEKITVTTSVRHEIKSKKRKRDTKDAGDKELRRTKKAKNKQEKKEEKKNKEKKDKKDKKEKKEKKEKKEKKEKKEKKXKKEKKEKKEKKEKKEKKEKKEKKEKKEKKEKKEKKEKKEKSEKKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZ82</accession>
    <name>A0A1E5RZ82_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g31</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZ82">
        <property type="interactions" value="515"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZ82"/>
    <dbReference type="InParanoid" id="A0A1E5RZ82"/>
    <dbReference type="OrthoDB" id="539158at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF32">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="17"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ92310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="535" mass="60818" checksum="A1E1F0BD3F083601" modified="2017-01-18" version="1">MVTNFSQIDXIPKIKDVVLGKTIGQGAFAFVKKASLKQDSNAVFAVKFVHLDTCKRHGLTDERIANEVLIHKKCSSNSNVIKLLDYNISKHYMWIAIELAEGGDLFDKIEPDIGLVDTELAQFYFQQLITAVDYLHSSCGVAHRDIKPENILLDKCGNMKLADFGLATVFRKKSGNIKWSSDICGSPPYMAPDILDPNGYRSDTADIWSCGVLLFVLLTGTTPWEEPTMNDEWYSVFMYSDGQILDGSWSKVDLMVLNLLRGILKPQTDQRFTIERIKHHKWFKHQVSFADGNCQCSNSEELFKRLTTPLKVSLRDEDYEQCTQQLKETCESTNNIKLPSKDKHKYVASQPLEGANAEAVILTHDIFDTDVIHAATQDPFFSLGKSSLPQDSWNTLLSQDPSVLQYQEKKDIIKNTKLFENNNIFTRFYSVESIENVLSMLESAIKTVDIKVHANLLQDYQSLLASLGPDLIFPLSMAIKTIDTRKLLLTGFIKIQTLNFPLKTITFVRTKGDPLEWRKLFKNITVLCRDIVFYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZF2</accession>
    <name>A0A1E5RZF2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Regulator of V-ATPase in vacuolar membrane protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g95</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZF2">
        <property type="interactions" value="83"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZF2"/>
    <dbReference type="InParanoid" id="A0A1E5RZF2"/>
    <dbReference type="OrthoDB" id="342131at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043291">
        <property type="term" value="C:RAVE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052208">
        <property type="entry name" value="DmX-like/RAVE_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022033">
        <property type="entry name" value="Rav1p_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13950:SF9">
        <property type="entry name" value="RABCONNECTIN-3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13950">
        <property type="entry name" value="RABCONNECTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12234">
        <property type="entry name" value="Rav1p_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RAVE complex protein Rav1 C-terminal" evidence="2">
        <location>
            <begin position="570"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1266"/>
            <end position="1380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1266"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1284"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1325"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1344"/>
            <end position="1355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ92380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1380" mass="157858" checksum="92A1E1D4AB401362" modified="2017-01-18" version="1">MSVTVLPGQPNDTQQAVCQANWQGHTILSYCSGNNVVVVTSQFRYFQTIYLSEDCHCCDLDEDTGFLAIGVSSKVVVYKPLSSIFTTKTPPKWVYCCTISQPNTGTVNCVKWSRLQSQKEIVIGSTYLSLWKIQDVYGEYKQTLLWTKLQSCPVYRVDITQDSQLICSMGKYDKTVRVWKRITLGTDASLFEVSILNHGNSAYVTSFRWKLLRQNWSDNESYSYVLYTLMSDKTLHIWTVYSLNNRKTIEIWGQINFDSGHRFAFIVDNCLLKSSRVKTDSDFLFTSDEKGHFDVYQMSHLSDNPPKLMKMRQHNRKVLVSGCFPLNANFLYFPEIKQFDSKDNCLSFIIHDVSGSVWHFFVDPQNLIAETENFEGSKPQEIEAEFQQVLTGHFKSIQKLVRSSDGESVLSLSRYASENCLWLPYKNGEIFSLRKFKVLQTEVPIKEAILLEKGKLAVLLLQNGKLQVWRSSENGTLHSTLMSTWDTLLANEPVLFVNVPTKVHNHDKHFIVLVYKDGSCKCFRIEHDKIHQVECESLTSEKIFNISAIDPVSYTYNGDRSIISVASVSGVIRCFKGVFETGEIKLKWAMTFEVMTGRKNITKFKGSSIDKMCCVDESQREVTIWDLKRGVLEYTEVFKEKVLDIDWTSSYGNMSIFSLGFLNHALSYTQVRYDYTHDYPSYAPIRKTDMTKYTSHAMGDSLWLGRGLFMIAAGNQILLKNKGLDLEHDPTARQTIGSRDIRFKNIFHLSGVLNGPLPVYHPQFLVQALYMGKINLVKEIMLKLLNEIRNCELLGASLHDLNSYLKLEPWKFLLKSDLNYTFDTTYEEPFDKFNSTLCQLLREKLTKTTLPDLTRHQQVTLITIIETLEKITNLGNSVDSYGINFIMGTKLYQSHKQSQPSITMRDVSWAVHCNQKDIIFSELNDIRMQWDKAKELKLAYWLSQDDLVVKTEQIAKMELSKNDERDPNACAIFYLALKKKHVLLGLWRICTGHPEQKKMLNFLNHDFSEDRWKTAALKNSYVLLSKHRDMMAACFFLLAGSLRDCVNVLVRKINDLDLAILVCRLFEGDNGPVLAEMLIQHILPSSISNNDRWSASFVYWKLKKKDLAIKSLIEDPAKLEXPKAGAKKIEVVNESFMVDDPVLLMMYMKLRNTHVTYFNSTLTIEESLEYNTLEKVCEVYRRLGCDYLALSLMKNWQFIDKQKLIKNENRQFTSTADSGLNEVPLLRPSLFDKFSQSWELPPSSSKETNVPVKSVFDGFGMSTDNVKSEAKCEQKENKWDGVNDPSNPSNDLGSYTNSKSTTSLKTHQSGPKNILDDFMQIPMTSNNSKGSEDSSSALEKSGNGKPSTVSLSASCVDSPHLQKYETSSTXQPRNLLDDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZS6</accession>
    <name>A0A1E5RZS6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g91</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RZS6"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ92384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1232" mass="141000" checksum="4028CFA70C9C5DCD" modified="2017-01-18" version="1">MGSLELLIEQINQKQQKPKQVFFFETGNGIPNRLETLFTKCEQYETGFPDKKAGRNDYSNMKFINTKKIFIIFVTSHSVTKNEICESDTLLNMSLQNTQLFELSPELQSKGDFIIHAHVVCNKSLFGEANSSGEIMLVITALGKLFLIDVSSMEVKGELTNFLKKLCSVNLEIYQLVNVKKSAFNQIKDICFVFQRQSLTLSKKHTFLFTKTFSEYDFEVCCSMILKSNKGRIADVVKTKYTDLQAPFKISYEDSCFIESFQLDQDYFVYLTSDPQKSIYKFHSLSTLNIRAEDSWKFHSSTPIGSKNFAQGTYKHEPLIVFVSNKLLALYTVISPDEQLWYLKNAVIDITKLDSLGIDKILKIELPRGDTPNKIYVYGETVMVLLHLEYDHTVFAKTKIRSVQKFDLPMKFLMIGDNYSITSNHDIVFFANVDEFHKLKIDQTSFFDDRILEAVFEKKLYSTSGVSLDPWDKHNFVPFHHTNYEHLEISGLTTSSFEFESNGIDANSVKLEDCYWSPGFDSLIMKATTDGIIFSNFCQIPHTNVSSGHQLLYTKYCKTITSYETSHKSSEILASANYFLNFENAELIIYLTSDGMLHLVKMSCESSIVSQTYPPLKIISEKMVTKASIIGDKFQVTXSNFSQSSNLILIACQQGVIGVENYQEFFNFNWPKELESLTICDFRXDNNSVANVTTSSGHIFELEVSTQNSLLKVQRKKHLSTEFQENSIVLDYHNTPSFYLSNNTLYDIKSLKPLQLPYVSVWFCHQHFHKATDKWLIYTKDNKRLEFTYDFELAKERSKVGFSQKVEAQPREFYVKEILLNSLNFRFSLVAVYSLTKSRNQKRFVKLVIFDLLTNATLKEYSAASLNISDTADIVSLRPLVNADDAPPKVTLKHLKKTGIDDKAYRESSALSVQLEQVFNKCFVVCLNYRDSGKRLKNELLIYHFNEHELVEVKEQAIKLQHRFTLLTGSRIAFCDRISKTELPSXLTEFPLFMTITEDGSLAQTWNVRYHPAEHRFEVVSQSEKHENTFAFAGSEEDTFVASKKFDHFTVLPCPFDAKAKSVYIGMTDTKSGRFQLLRYEYGHSLQWLTKTEQLVCLDKFTVGQHVSMNTNKIIYASECTVIMLVNGKKDIIVANYSSGMEANLSLASHKKRKLNPASLQKHCVXDFCDLSPFGNSQYTASAEESFRLTNSYGHFTSVDVIGKEAHGLGSAVLYLLLSVSHTRTYRLKIKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZW8</accession>
    <name>A0A1E5RZW8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Reduced viability upon starvation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g138</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZW8"/>
    <dbReference type="FunCoup" id="A0A1E5RZW8">
        <property type="interactions" value="451"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZW8"/>
    <dbReference type="InParanoid" id="A0A1E5RZW8"/>
    <dbReference type="OrthoDB" id="446293at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990528">
        <property type="term" value="C:Rvs161p-Rvs167p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07591">
        <property type="entry name" value="BAR_Rvs161p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000014">
        <property type="entry name" value="Protein hob3, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046982">
        <property type="entry name" value="BIN3/RVS161-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037429">
        <property type="entry name" value="Rvs161/Hob3_BAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174">
        <property type="entry name" value="BRIDGING INTEGRATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174:SF3">
        <property type="entry name" value="BRIDGING INTEGRATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAR" evidence="4">
        <location>
            <begin position="15"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ92295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="264" mass="30373" checksum="2766067B3F27C475" modified="2017-01-18" version="1">MSWTGFKKAINRAGNSVMVKEVDKTVDKEYDTEERRYRVLERTGGALQKEAKGFLDSLRAVTASQVTIAEVISNLYDDSKSVNGSYNVGNYYLQCVRDFDSETVKQLDGPFRETVLDPITKFSNYFNEIDEAIKKRAHKKQDYDACKAKVRRLIDKPAKDASKLPRSEKELKFAKDIFDNLNDQLKAELPQLVSLRVPYFDPSFEALVKIQLRFCTEGYTRLAQIQQYLDEQSRDDYAHGRLDTKMEQVLQQMSSLDICALGFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S081</accession>
    <name>A0A1E5S081_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N(6)-L-threonylcarbamoyladenine synthase</fullName>
            <ecNumber evidence="1">2.3.1.234</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">N6-L-threonylcarbamoyladenine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g7</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-threonylcarbamoyladenylate + adenosine(37) in tRNA = N(6)-L-threonylcarbamoyladenosine(37) in tRNA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5S081"/>
    <dbReference type="FunCoup" id="A0A1E5S081">
        <property type="interactions" value="598"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S081"/>
    <dbReference type="InParanoid" id="A0A1E5S081"/>
    <dbReference type="OrthoDB" id="10254073at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:tRNA N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd24132">
        <property type="entry name" value="ASKHA_NBD_OSGEP_like_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000038">
        <property type="entry name" value="Probable tRNA N6-adenosine threonylcarbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000295">
        <property type="entry name" value="Probable tRNA N6-adenosine threonylcarbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01446">
        <property type="entry name" value="Kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034680">
        <property type="entry name" value="Kae1_archaea_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017860">
        <property type="entry name" value="Peptidase_M22_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF14">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01016">
        <property type="entry name" value="GLYCOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Gcp-like" evidence="11">
        <location>
            <begin position="79"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="186"/>
            <end position="190"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ92415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="409" mass="45683" checksum="2AE271C44C94758B" modified="2017-01-18" version="1">MYSCFTHLIPTNRTATQSSTNKTKYHKLSMVNINGIKPKNGSYYLCLGLEGSANKLGVGIIKQSYNPDFPEKEFTDTEILSNIRNTYISPPGEGFMPRDTARHHRNWIVRLIRESLDECGLKDLSAVDCICFTRGPGMGAPLYSVVVVARALSLLYQVPLVGVNHCIGHIEMGREITHAANPVVLYVSGGNTQVIAYSENRYRIFGETLDIAIGNCLDRFARTLKISNYPSPGYNIEQLAKKSDRSKELVELPYTVKGMDLSMSGILAFVDRLAKDLFKGPKSGNKKLFSKSGEPLVTVEDLCYSLQEHLFSMLVEITERAMAHVGSSQVLIVGGVGCNVRLQEMMGLMCRDRKGMCYSTDERFCIDNGVMIAQAGLLQYRMGDVVQDFKDTVVTQKFRTDEVYVSWRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S0B3</accession>
    <name>A0A1E5S0B3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ornithine cyclodeaminase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ornithine cyclodeaminase/mu-crystallin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5S0B3">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S0B3"/>
    <dbReference type="InParanoid" id="A0A1E5S0B3"/>
    <dbReference type="OrthoDB" id="41492at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1780.10">
        <property type="entry name" value="ornithine cyclodeaminase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003462">
        <property type="entry name" value="ODC_Mu_crystall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023401">
        <property type="entry name" value="ODC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812:SF19">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="375" mass="41991" checksum="0A292C22ECEF2603" modified="2017-01-18" version="1">MKYVTDEQQERLFLDACSPEQVLEYQNLLGSALEEYSQDPSLNPPRMVSQNPHGTTILHMPISSDAYNGIKTLAYNPQXNLGFVGNVSCLNKKTGELEGIVSAKVLTGFRTALASCIGLQKVLETLEWNHPPALDLMHSQDHLKNIQITVLGSGLQAFWHIVIAIKNLLVYKSAVSNIQINIFYRRNRMSDENVAKILNQGNIVQVTQYKHDIDNGTDSEDGLISEKISKSDIIFGCTPSIHPTLKFKYFQKLTMQETVTRTGFFKKNKVTYISLIGSYKPEMHEADNDFMQFFTENGKQNIIVDSTEQVLIEAGEFIDSNVQPQSLVEIGKLIKSQSPVPTVYSKDLDRSFVFCKLVGLAVMDISVAQHIIASI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R013</accession>
    <name>A0A1E5R013_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA wybutosine-synthesizing protein 4</fullName>
            <ecNumber evidence="5">2.1.1.290</ecNumber>
            <ecNumber evidence="4">2.3.1.231</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">tRNA(Phe) (7-(3-amino-3-(methoxycarbonyl)propyl)wyosine(37)-N)-methoxycarbonyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g4656</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>7-[(3S)-(3-amino-3-methoxycarbonyl)propyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine + CO2 = wybutosine(37) in tRNA(Phe) + S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:37119"/>
            <dbReference type="Rhea" id="RHEA-COMP:11844"/>
            <dbReference type="Rhea" id="RHEA-COMP:11847"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73544"/>
            <dbReference type="ChEBI" id="CHEBI:74275"/>
            <dbReference type="EC" id="2.3.1.231"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-(3-amino-3-methoxycarbonyl)propyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:36903"/>
            <dbReference type="Rhea" id="RHEA-COMP:10379"/>
            <dbReference type="Rhea" id="RHEA-COMP:11844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73543"/>
            <dbReference type="ChEBI" id="CHEBI:74275"/>
            <dbReference type="EC" id="2.1.1.290"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">tRNA modification; wybutosine-tRNA(Phe) biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methyltransferase superfamily. LCMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.290" evidence="5"/>
    <dbReference type="EC" id="2.3.1.231" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000017">
        <property type="protein sequence ID" value="OEJ80245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R013">
        <property type="interactions" value="113"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R013"/>
    <dbReference type="InParanoid" id="A0A1E5R013"/>
    <dbReference type="OrthoDB" id="47172at2759"/>
    <dbReference type="UniPathway" id="UPA00375"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007213">
        <property type="entry name" value="Ppm1/Ppm2/Tcmp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46529">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46529:SF1">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13418">
        <property type="entry name" value="Beta-prop_TYW4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04072">
        <property type="entry name" value="LCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="15">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ80245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="667" mass="75325" checksum="7A6099AD14CB3EF8" modified="2017-01-18" version="1">MGDPSPQAKEANKNKKGTKKPLKKTANKSKFEDLAIQGTNNSSIASKLSVERIYFQKLKTNVSIENEDKVIPYFQWFVGKAIRRSPCINRGYWLRLEAVRSRLWDIIKSSDKKKICIVNLGCGFDPLPFEVLDPKNSNKLTQYNLQNNKPISFVDIDYSDLLDAKKNLIANNPHLENIMKENGDNYKMVTCDLNNGADYARLLENEIPADTIKVFIAEVSLAYMKPQYADRVIFESGKLANSHFILMEQLMPQGPNEPFARQMLKHFKKNESPLQSVGTYQTIKQQISRFEQHGWSKINCSDMYELWENLSPELKNSVEQVEPFDELEEFSLFAHSYIVLHATNNKNYEFCFKFQEPMAASIDDYSFASSNXQTELTWEDGDYERKFGASSEDGLYCGGYSNARLNDIIDTKTGETLKCKGAVQPEGRVYHTFTKVSETKYMLLGGRTAPNKVLRDCWFWENSQWTKSEFEAPEPLYRHSVMTLSNGSVLILTGCGATHHYLFNEHTGFTVAESDLPHVNSGALCHQNNRGFLTGGELESGLVSSAMYTFDVDDQNSAISIVTVSHSPLFRRYAAKSAIYQGNNVLLVGGISDKYVFGQNSSMVLVENGNATVLKMADDIWENSMVLCGFEMQAVGKDEYVVFGGGAVCYGFGSIWNNRKLRIKIGX</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R095</accession>
    <name>A0A1E5R095_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ribosome biogenesis protein BRX1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4472</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BRX1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R095"/>
    <dbReference type="FunCoup" id="A0A1E5R095">
        <property type="interactions" value="1203"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R095"/>
    <dbReference type="InParanoid" id="A0A1E5R095"/>
    <dbReference type="OrthoDB" id="1638493at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026532">
        <property type="entry name" value="BRX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13634">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13634:SF0">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BRX1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Brix" evidence="6">
        <location>
            <begin position="36"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="311" mass="35807" checksum="B0E7B6CA28397502" modified="2017-01-18" version="1">MSSIYKALTGTKSKKNAESSSNSKSQVTEKKWMNKQRTLLISSRGVTYRHRHLISDLNSLLPHSRKEPKIDSKKKIGTLLPELSELYNCNNIMYFEARKHQDLYLWLSKPPNGPTIKFYLQNLHTLDELNFTGNCLKGSRPVLSFDAQFDDKPEFKLIKEMLLHNFGVPPQQKKSKPFIDHVMSFSIVDGKIWVRNYEIAHTASNKDKYEADDEITKKKTRTGEDTEEEISLVEIGPRFVMTPIVILEGCFNGPKIFENKQYVSPNVARSQMKQQLAKEAQLRSEAAVKRKLQKRENVLMADPLSNENVFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0C5</accession>
    <name>A0A1E5R0C5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4430</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0C5"/>
    <dbReference type="FunCoup" id="A0A1E5R0C5">
        <property type="interactions" value="146"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0C5"/>
    <dbReference type="InParanoid" id="A0A1E5R0C5"/>
    <dbReference type="OrthoDB" id="73901at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF27">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN CTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0186">Copper</keyword>
    <keyword evidence="5" id="KW-0187">Copper transport</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="278"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="287"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="299" mass="33773" checksum="5EA056DD27643540" modified="2017-01-18" version="1">MNMNMGTTSSAMDATATSSSAQTTVTMMMSMDMDMNMNTYLTSNYLHYPVLFKHLQAHTEAQAFGIFCLILATTLVYKLTGFVLWYLELTWAAKTNENITEEQQLLAAKHRNNRIMEFFVPSARTFLQDTVRLVLLFWSTLLVYCLMLVSMTFILTYVFAVVLGLSLFEVAVNRIKIIVLRERRLRALKKCDDSDKLDDCACGDDSEDSDARLDETDAKTNIFQKEDTSNASETTTLPEDRMSIKSKPKKASCCCAPSAENPAEQELEQEIQMANEIGENGRANADQMDSSLEPRNQFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0N2</accession>
    <name>A0A1E5R0N2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4619</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R0N2"/>
    <dbReference type="OrthoDB" id="3631276at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="853" mass="98726" checksum="7ECC176F2167F5FB" modified="2017-01-18" version="1">MPSGKXKYERLANPADDDFDINDDNDEDVLFELDKTTSSARHKGDDNDNDNDNDFEEYGKTYHQSNGKTASKNGTDDFDLSFSDNKQNPLGWKINTVSSKIIRLFKNYRMWNTNMWKMGVITGFGTLIVVLIIIGIFKSSSMIVSHHDINNHGDVSPYGHHEPHQEQDLFSYYTSWLDSFYAPSEEMDSEHNKKNKKGGKNKLDSKLSSFNSISENYGINIFKYFKIINRSLNIKDTDFIYNLDKSLESSNKDNIKELSNGVDDYYVDYSNNEEIHHMKHEPLTISAMGNKPGWTKDYEFYSKKIQEVFKEKYMKAVDFDYFADGEHEFKILKTESIWLPQENVFFVSSIVEYSFTKYSKKIVSNHVLMQLFDENYMEVQDKRLFNKDFYANTDDDDFEVERKMNGKIEELVHYLKFIDEESNVGNCDQYLGDHPEKGKDDEPERMKFDKCIANSLKNFHKFETYKENMLSKFSLSFPAILEIPQLASPKKLKKTFESLPKEQANSEDSNSPPEYFVGPQDVQLILDIDNQDLHIILTTSTDFYTKSTKNKLEKMKMFDIKLFNKVNSFIELTNVVSGKSIYYDSGSIVSANKQVFTPMFEHNKLVFITSLVSNNKKEIDTEIQFLECSLEDGVCFNNELNEQLISGNNAESASQQTIXHGFRIMTNFIEVPSXLFFFLDPHHLYSHSYXXKVFLAFIKYDESRLCDGENGDKFGTVIVESFTNDNGLVFRTVAISKPWKIPQLESKDNSVTRITSIPFWSVELDPANSQDCMKHGWPYRNCRLKDHLALHIMRSNGFSQQQISNDETNELVVINGLQNMLESAIDNIRHKNNRSPQSRASANPLMLMENMCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0S6</accession>
    <name>A0A1E5R0S6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Subunit of the CCR4-NOT transcriptional regulatory complex</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4571</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0S6"/>
    <dbReference type="InParanoid" id="A0A1E5R0S6"/>
    <dbReference type="OrthoDB" id="3980110at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="877"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="932"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="878"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="900"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="932"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="945"/>
            <end position="957"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1404" mass="163008" checksum="8775D675A55DE4E9" modified="2017-01-18" version="1">MAKKTKKGRSSHQQHGNKGQNXNGSNGKNNTNNTPSDGVNTENKNINGDATAQPTRPKKKMSKLELERFQEXQRRRKLKELAKKNSFVEENDDLDVQEFKKWMYXDYDYECFKLFTGKIQPNTPPVVKISLLTRYSGLDKKIENIPNKDNITYITPEXTHIINFETSGFVVKPNNEEGTSKESMQXFLAKEIMKNSFGKTITECLDMLYLVLKMFLFQNNTFSVKLLERLTTTVLTISEYNADMXLNNNQQSSQKELDPNKKKVALFQRQIRQNYCRLYNIDAKQPLLTWEQFEALLKTITQSPNLYNNDEXFLEKMSQWIIYNGIENESLRKDLLNDQDYNAFVDLFQMDAHYPSIIEFILGIFLRYCMIVXSEMKPLKNKIPGSSETXYTQTDLLITNVLKSLSGDIDHQSFQQHXTNLLARLNSFNQYLDTVLHLKYNFMNFLILNPTPNIFSQHYDHLRAEYXTNEDEVIGEDIDDLSIIKDNKQLKLLQKNETDIFQDLYEEEDVFVLHDIILNALFPQKTSINNPLTRKLVSILISLADPKTQPLPTDTTIVSIDYLSDIFLGSVWIFYMKDQCAGSGPQSETLYFKLSMLLITIIKATLPLLKLNSPDIKLDADCFESARQKINENWKANYTSWFPCDTDLRTLEILYMMNILATFAIFKLTLATGKXCDIHYKISPFSTFFIKLWCFQSFQIHQGILIDQYEEEKMTCETPNIVKAVIRGNTAWRCVNALLINTSDSAFSNXLGDQMDKYYTLVKHDYEHEPFNSFMSPHGRKLSTGALTIKEMKYEIITNLYTQETPFTTPEITQETVTNNKENTEDDENSADLAYAYMQNNFTELFPWPIFYADRYDGEARYMFDWEYDLYNEPEVSEPGKHLGNDAAHDGNIKSMTPADKNNQSPQQTSHPGAPRCYCLIDVFXDEDEELIEEDPVEKDPVEEEEKRQLEXTRATETKNPSTRFEDLLPEELEEVDYDIIKNTIPTGKDPDGHYWYDKDYFEHRFELSPVPSEPLAKVSATPESLLTTTPSLFHALKDPHHCEYFVWVVCELLKDQKMREVXNIQRFVLELLYEFHEHLTSRTRALELRKIRVYLKWFFLKNIDLVFHYFREPXTRNFAVILLNKLLEDPANYAILEKLILNYTSQDYYFLVFVLKSLVHDRHAMYTKVPNFDVSSVEVSXQLSMKQCFECLSNIVLTAVSTHINEHETGTENCVLIAKTMVEFFKILKSKPERIAHKVFSEYIQPYTSAPEHLQGNSGSSTKDGPSEAIEVQNFLASLFSFQYQLGECLPQSNRDLIFEYFNAIVSLLNNPQWDQLVEEDYFSKSELETEGTEELKNTTETLQHLALDKNCFLDFCKEFHIFAVDPKQKSLVHPYEHKSXIKPLATPLSCSLESYLALTTSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0V8</accession>
    <name>A0A1E5R0V8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transport and Golgi organization protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g4580</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0V8">
        <property type="interactions" value="378"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R0V8"/>
    <dbReference type="OrthoDB" id="191601at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008551">
        <property type="entry name" value="TANGO2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17985">
        <property type="entry name" value="SER/THR-RICH PROTEIN T10 IN DGCR REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17985:SF8">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05742">
        <property type="entry name" value="TANGO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ80526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="281" mass="32252" checksum="89426A060906EB88" modified="2017-01-18" version="1">MCILMGMTNHPDYSLVLISNRDEVFERETETAALRFDDQVVCSIDKLNGGTWLGVNVRAKKFAVVLNVQTNVRALPITKSRGALPMVYLLDPRVTPENSLNGFEDFAQIYPDIENTRHFSLVYGFFDTTPVQPTVISVGDDNIARSRKLVEERLFVLTNNKLDQDFEQWPKYQFGLDALKNLHRHSGDKLIERCLEISTTSTFPAIDFSKEYTFQESKKFVKQSIFIPKHKVNIDGKHFTYGTRTTTVIMVNRKTGSIDYVEHDNTSCQTKXFHFTKPQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R175</accession>
    <name>A0A1E5R175_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin-conjugating enzyme E2 1</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">E2 ubiquitin-conjugating enzyme 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g4307</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="9">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R175"/>
    <dbReference type="FunCoup" id="A0A1E5R175">
        <property type="interactions" value="1177"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R175"/>
    <dbReference type="InParanoid" id="A0A1E5R175"/>
    <dbReference type="OrthoDB" id="9993688at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23800">
        <property type="entry name" value="UBCc_UBE2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000037">
        <property type="entry name" value="ubiquitin-conjugating enzyme E2 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015368">
        <property type="entry name" value="UBA_C_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09288">
        <property type="entry name" value="UBA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="10">
        <location>
            <begin position="2"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="8">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00072431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00077197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ80640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="218" mass="24334" checksum="31E669C03B004D99" modified="2017-01-18" version="1">MSTAKRLLREITSVKEDPEANITLSFNNESDIYKLTGSFLGPPDTPYEGGIFLVDINVPTDYPFKPPKMQFKTKIYHPNISSVTGAICLDILKDAWSPVITLKSALISLQALLQSPEPNDPQDAEVARHYIKDKEGFEKTARLWTKLYATNKTSTNEGQDYTEADLYGIDSDLCSIFLAKGYPLSNIVTVIRRAGIKKIADLDATVQSRIEDQLQKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1A5</accession>
    <name>A0A1E5R1A5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">pyridoxal 5'-phosphate synthase</fullName>
            <ecNumber evidence="7">1.4.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">PNP/PMP oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g4205</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>pyridoxamine 5'-phosphate + O2 + H2O = pyridoxal 5'-phosphate + H2O2 + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15817"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58451"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="1.4.3.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>pyridoxine 5'-phosphate + O2 = pyridoxal 5'-phosphate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:15149"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:58589"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="1.4.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxine 5'-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the pyridoxamine 5'-phosphate oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.5" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1A5"/>
    <dbReference type="FunCoup" id="A0A1E5R1A5">
        <property type="interactions" value="419"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1A5"/>
    <dbReference type="InParanoid" id="A0A1E5R1A5"/>
    <dbReference type="OrthoDB" id="303614at2759"/>
    <dbReference type="UniPathway" id="UPA01068">
        <property type="reaction ID" value="UER00304"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004733">
        <property type="term" value="F:pyridoxamine phosphate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.110.10:FF:000010">
        <property type="entry name" value="Pyridoxine phosphate oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01629">
        <property type="entry name" value="PdxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000659">
        <property type="entry name" value="Pyridox_Oxase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019740">
        <property type="entry name" value="Pyridox_Oxase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019576">
        <property type="entry name" value="Pyridoxamine_oxidase_dimer_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00558">
        <property type="entry name" value="pdxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004231">
        <property type="entry name" value="PRK05679.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10851:SF0">
        <property type="entry name" value="PYRIDOXINE-5'-PHOSPHATE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10851">
        <property type="entry name" value="PYRIDOXINE-5-PHOSPHATE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10590">
        <property type="entry name" value="PNP_phzG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="PNPOx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000190">
        <property type="entry name" value="Pyd_amn-ph_oxd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01064">
        <property type="entry name" value="PYRIDOX_OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0288">FMN</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0664">Pyridoxine biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyridoxamine 5'-phosphate oxidase N-terminal" evidence="15">
        <location>
            <begin position="73"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridoxine 5'-phosphate oxidase dimerisation C-terminal" evidence="16">
        <location>
            <begin position="203"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF10590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ80688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="245" mass="28340" checksum="8C2661B87177050B" modified="2017-01-18" version="1">MSTFNLAFNNFIHMSSDNLTTDEKTETPIIFAPKTYQYDKFALGESDCPKDPIELFTAWFNEAREDPTETIPEAVNVATAELPSGRVSNRVLLFKELDPKGFCIYSNWGSSKKAHDVKTNPHAALTFFWKNLQKQVRVEGVVEYVSRETSERYFETRPRGSKIGAWSSPQSKVIKNREELENIVEANKKKFENVEDVPCPDYWGGIRVVPLEIEFWQGRASRLHDRISYRRKDENSPWEILRLAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1B8</accession>
    <name>A0A1E5R1B8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutaredoxin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3963</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1B8"/>
    <dbReference type="InParanoid" id="A0A1E5R1B8"/>
    <dbReference type="OrthoDB" id="429967at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008554">
        <property type="entry name" value="Glutaredoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052565">
        <property type="entry name" value="Glutaredoxin-like_YDR286C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33558">
        <property type="entry name" value="GLUTAREDOXIN-LIKE PROTEIN C5ORF63 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33558:SF1">
        <property type="entry name" value="GLUTAREDOXIN-LIKE PROTEIN C5ORF63 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05768">
        <property type="entry name" value="Glrx-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="176" mass="20298" checksum="80B3B3BA64A26082" modified="2017-01-18" version="1">MIATKSVMQPYXLNKSVYTGTFKSLLKQNTSISRTPIKQYTSVWNPSIRQYTKVATPYVNASERARVNMTLFSKDNCQLCDKALYVLDEIYLKKPEYKEMVGLEIIDINNPLNKEWWDKYCFDVPVLHISEVNTNKGINDRAIEDTEVGDTGANNVTKIFHKLDENDVITKINRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1F7</accession>
    <name>A0A1E5R1F7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">E3 ubiquitin ligase complex SCF subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g4106</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the SKP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1F7"/>
    <dbReference type="FunCoup" id="A0A1E5R1F7">
        <property type="interactions" value="857"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1F7"/>
    <dbReference type="InParanoid" id="A0A1E5R1F7"/>
    <dbReference type="OrthoDB" id="2342932at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18322">
        <property type="entry name" value="BTB_POZ_SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000133">
        <property type="entry name" value="Suppressor of kinetochore protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016897">
        <property type="entry name" value="SKP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001232">
        <property type="entry name" value="SKP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036296">
        <property type="entry name" value="SKP1-like_dim_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016072">
        <property type="entry name" value="Skp1_comp_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016073">
        <property type="entry name" value="Skp1_comp_POZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11165">
        <property type="entry name" value="SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01466">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03931">
        <property type="entry name" value="Skp1_POZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028729">
        <property type="entry name" value="E3_ubiquit_lig_SCF_Skp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00512">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81382">
        <property type="entry name" value="Skp1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SKP1 component POZ" evidence="5">
        <location>
            <begin position="5"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="SKP1 component dimerisation" evidence="4">
        <location>
            <begin position="127"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR028729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ80739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="177" mass="20429" checksum="20ED8EFF795F530A" modified="2017-01-18" version="1">MAEQKVTLVSGEGEKFEVERVIAERSLLLKNFLSNMLNSNIDDDDTADANKNESEEEIVIPVPNVRSSVLEKVIQWAEHHKDSKFPDDDDDDSRKSAPIDSWDREFLKVDQEMMYEIILAANYLNIKPLLDSGCRVVAEMIRGRTPEEIRKTFNIVNDFTPEEEAAIKRENEWAEDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1H1</accession>
    <name>A0A1E5R1H1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">tRNA-specific adenosine deaminase subunit TAD3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g4086</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidine and deoxycytidylate deaminase family. ADAT3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1H1"/>
    <dbReference type="FunCoup" id="A0A1E5R1H1">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1H1"/>
    <dbReference type="InParanoid" id="A0A1E5R1H1"/>
    <dbReference type="OrthoDB" id="3971551at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052717">
        <property type="term" value="F:tRNA-specific adenosine-34 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01285">
        <property type="entry name" value="nucleoside_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079">
        <property type="entry name" value="CYTOSINE DEAMINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079:SF156">
        <property type="entry name" value="INACTIVE TRNA-SPECIFIC ADENOSINE DEAMINASE-LIKE PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="3">
        <location>
            <begin position="166"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="312" mass="35530" checksum="CB8FCFE1B061AC43" modified="2017-01-18" version="1">MVKKIHNPVKIDYVNCLVEDCLQQIKGADSMTTSAQPHEFLSCWVIEIKNKALVQKFVRFAKENIPSNELDGLQNVKKIKVENGALKFIPCSTDTIQTKEKLQELIAEHTKADANDYLLLDQLALIPKTSPPTKEIALEWGKSYWPLTWRGNPNDQVLNDMEYDMVKINKYLKEISELSATCKNTVPSVTIFSNSRQNKLYVAYDERDTTGNPLDHSVMCGIKQVAENLPKDGYLCLDYEVFTTHEPCAMCCMALVHSRISRLFYIQDVKHTGSLKSNYHMHGIKSLNSKYHVFQYVGDKYHAAAVEQSAFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1R3</accession>
    <name>A0A1E5R1R3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4245</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1R3"/>
    <dbReference type="InParanoid" id="A0A1E5R1R3"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="200" mass="23291" checksum="82963335BD2CDC4A" modified="2017-01-18" version="1">MRSYNYTTMPYFYYYCSITLAFLMGFLLKRKLTATSGKDGDKTANNADDTVECGLKGKSIKENSLPKTNSPVAVCEKSCFTPAFSSLKFEHSTEIDEDLTEEYFHNYTSTGKNYSHFDDMDNMSKSSIRLWRKLFITPNHVKLLPSQKLNTFNSQYAFEGFKEFHTVKTWRNVSNFKPREKRQQSDYEKTPPLQIVSMFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R229</accession>
    <name>A0A1E5R229_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Retrograde regulation protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4385</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R229"/>
    <dbReference type="FunCoup" id="A0A1E5R229">
        <property type="interactions" value="294"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R229"/>
    <dbReference type="InParanoid" id="A0A1E5R229"/>
    <dbReference type="OrthoDB" id="2014654at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000191">
        <property type="entry name" value="Retrograde regulation protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050273">
        <property type="entry name" value="GppA/Ppx_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003695">
        <property type="entry name" value="Ppx_GppA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057512">
        <property type="entry name" value="RTG2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30005">
        <property type="entry name" value="EXOPOLYPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30005:SF0">
        <property type="entry name" value="RETROGRADE REGULATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02541">
        <property type="entry name" value="Ppx-GppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23566">
        <property type="entry name" value="RTG2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ppx/GppA phosphatase N-terminal" evidence="1">
        <location>
            <begin position="33"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="RTG2 C-terminal" evidence="2">
        <location>
            <begin position="348"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="580" mass="63423" checksum="6D2E09D177E55FB6" modified="2017-01-18" version="1">MSNSVNNTSETEVVSRNLCGVVDIGSNGIRFSISSKAAHHARIMPCVFKDRVGISLFDVQYATLNAGKAPIPTQVIKDVCAAMKRFKLICEDFGVPQTGVRVVATEATREALNSKDFTAAIEESTGWQVELLAKEEEGRIGAYGVISSFNIASGLFIDLGGGSTQLSWIQCINGEVKMSSSPVSLPYGAGALSRRMQLEDKRSLFLEIRQAYVDAVKKIGIPDDMIKDAEAKGGFDVYACGGGLRGMGHLILSRIEEYPIQTIINGFSCSFEEFESICDYLFLKGHIPGNTKIFRVSDRRAQQLPAVGLLMSAAFESLPKVRNIHFSEGGVREGSLYSILPREIRAQDPIHIASRPYAPLLASKYLALLKTAIPLSEVPPMVYNRVAPALCNLAFVHAGYPKELQSTAALHVATTGIIAGCHGLSHRIRALVGIALCERWGGDLPQTELDYKQKLEDVVLRDGDKAERQKLVWWTKYIGQIMYVICGVHPGGNIRDGVFNFTINESDDMDVTDNAGVPVSGSKKKALEVIVKISREGLKTSASVRGRIMTLQKKIRKLSKGSSQKVKVGVQYFEKEDENT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R266</accession>
    <name>A0A1E5R266_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g4240</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 12">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R266">
        <property type="interactions" value="488"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R266"/>
    <dbReference type="InParanoid" id="A0A1E5R266"/>
    <dbReference type="OrthoDB" id="282152at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10509">
        <property type="entry name" value="Zn-ribbon_RPC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034014">
        <property type="entry name" value="Zn_ribbon_RPC11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF12">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 9 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="13">
        <location>
            <begin position="67"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="10">
        <location>
            <begin position="5"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="5"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ80995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="112" mass="12990" checksum="99F4FC05D14B179A" modified="2017-01-18" version="1">MLSFCPNCNNMLLVEKNEFTGIYNLSCPSCPYNFIIDGNIXVYDRKKLPRKEVDDVLGGGWDNVDQTKVQCPNYEKCGGEKAYFFQLQIRSADEPMTSFYKCVECGHRWREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2H4</accession>
    <name>A0A1E5R2H4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4210</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2H4">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R2H4"/>
    <dbReference type="OrthoDB" id="3971929at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000747">
        <property type="term" value="P:conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033481">
        <property type="entry name" value="Dni1/Fig1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28092">
        <property type="entry name" value="FACTOR-INDUCED GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28092:SF1">
        <property type="entry name" value="FACTOR-INDUCED GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12351">
        <property type="entry name" value="Fig1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="183"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="261"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ81106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="304" mass="34102" checksum="DC2CE56E944D64F6" modified="2017-01-18" version="1">MQNLNQISVKPGLSGICLDNLPKTHFNDSTQCYKKNYNFTSVXPNNIGTTGTNFTYGLHDILNIEILTIRSTNTSSSTLSADLNILEIAQDQTKLVHPSMTIVSLTFQVICMAVLLFTMIPALIKIPNTKSPLTLYLTIGVTFLSCCVNGFNGMLIQTNIDTVALLVPKSSMHILQVTKGTKLISMVWASFAMHIMQLFLLGYAAYRLVSKLDDDSDDNTFASKYDKEMEEDKEALKLFSEKLQREKFGSHRKDPNNKITSPSRNSSFASSWNSSRKNNAKHDKSNKNHYLYTYHPNDSSSTYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2I1</accession>
    <name>A0A1E5R2I1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein HOL1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3937</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2I1"/>
    <dbReference type="FunCoup" id="A0A1E5R2I1">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2I1"/>
    <dbReference type="InParanoid" id="A0A1E5R2I1"/>
    <dbReference type="OrthoDB" id="5215911at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000224">
        <property type="entry name" value="MFS transporter, putative"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF34">
        <property type="entry name" value="PROTEIN HOL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="241"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="418"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="461"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="501"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="534"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="563"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="599"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ81117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="641" mass="71326" checksum="7BCBCB6078D2B701" modified="2017-01-18" version="1">MVDSTSLEQFTDPLHPDYVPGTFNIYLEMGQDSQAYDEEAYAQSGDDSASNIFSNHMKKDKKSGIVLMPQPSDSPNDPLNWSYWRKAWHFALMAYITAFTAATSNMAGATQDALNEKYGISYNSMNTGAGVLFLSIGWGTLFCAPFANLYGRKLTYLICIVMGLCGAIWMGLSSRVRDTIWSQLFIGASEACAEAQVQLSLSDIFFQHQLGSVLTVYILATSVGTFLGPLIAGYISDLTTFRWVGYIALIISGGTLLVFVLGCEETYFDRTLYKTPMNTDPLEGQHLEGIAKYPSNSDNSHGADEKLQNKINVSTAIKKKKENDIDAQHPSDIQQILSQVYSNNIPEAEIGHDKISPLATATADGNGIPGLNLELIDGSLESPKTYLQRMALITKATNLRGWGFKQYFQYMYLNMRMFAFPAVWISGLFWGWQDVLLSFYLTIEEDNYYDDPWDYSDAAVAIMNVPTLIGAVIGCMYAGVISDHFVVWLSKRKNGIYEAEYRLYFSFAVAILSPAGLFMFGIASGRGFTNWRTAYMGLAFIGFGWGCAGDIAMAYLMDCYPEMVLEGMVCTSLINNNLACIFTFTCSDWLDASGIENTFIALGVLTFFFTSLALPMFIYGKKARKWSKDRYLAFISIRDGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4H2</accession>
    <name>A0A1E5R4H2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein KRE1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3824</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4H2"/>
    <dbReference type="STRING" id="56408.A0A1E5R4H2"/>
    <dbReference type="InParanoid" id="A0A1E5R4H2"/>
    <dbReference type="OrthoDB" id="5406216at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031452">
        <property type="entry name" value="Kre1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17056">
        <property type="entry name" value="KRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184599" evidence="2">
        <location>
            <begin position="19"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ81816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="337" mass="34367" checksum="196A8DFB5C5F518C" modified="2017-01-18" version="1">MKLTKLAFLLNLLAVGFCSVLSTYTYITTNANGGPLTIYSVVTLPDPTTTPTTTTTTGATTTTAGAATTTPTTTAVTNTATTGTSSSKSSTGTTATGTATDTATDTATNTATDTATGTDQVVTSTMTQFTTDANGQTITLYIATTYTGTPAKITSSTGTATGTATGTATGTATGTDTATTNTATTATSFSSSSSAKAAAAAITDSNDLTKPDPSTNLTPLPTSPVTTLSLHSLLTMELAYTTYTTTRAKTSMWVTVTTDGYVTVFETTFAQRFTSQYSASLVPSSGSVGMGTLTGTFGVVKDELKVTYADNAISNSARKGLFMTLLTSIMTFFMFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4J7</accession>
    <name>A0A1E5R4J7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3743</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R4J7"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="534"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="534"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="785" mass="88467" checksum="B2EF2899D9DDC887" modified="2017-01-18" version="1">MVTEHFKRTENTEENMKIVDRYANAIXFEPNKDVVFLLFKLLLKLPSPATDASPSLGAYKKNKYSLLFSSIAPVPRGKNANFEDVMFFMQRMMRSSSLLLDLVKTYCLGFQXSQLENFNEKMNPFDFLQLLSVVSSSNSKYSNKEKNEINERIDLLEKAXVEASRNKLSNFSGLFAEGIAYILIPMILHKGKREVFLQTIIPEKLLLSLQRIXXVAQSPTNKNNSIEFQHFKTKSENLMLKHTNXNLKNSLTXMKDXTENCKAHISCSENDAFKVKSSDLSTQESAHFVTRSITQLESSYFNGVSENLLFSESQEFLLCYNYLCDLQKGLIYLNENLNQTVKXSHAGPILVEQRNLVQDLIRSCHFFEIKDDCIFKQDVPTLDNSFESAERLVPYAXEEDPQKASASASHEERKXNVXKDDVTIPNVDXFNQXSFSKLNTAAGEAEKKEQDAGNALAELTLMDHSETEFNSIKGRTNLVPKKDKLHVKFDLLGNSSDAFXRNEKLDKAQQNSPSGSSDSEDVFFSYSSTADLLKPKDNXKAKSGDLGNALKVGSNSKKAKCKNKPSSKPTVFLSPTSNLFGPPSQCHTNLLPPVPLIAGPPLLASLQQNSNFKPLKRKSGGQFPLMDSALKNTQSVKLAKNFKVNYSNKNCLITFRSKSAGAFDFLSSSKKNRDRYFYSSEFSASDETLDGNENXLYSSTKDQPVVNKELFKXKHAKLDINQTWLPITNHPLYAGSISGGHXWWIRSGKNLSYEDQIKYIGDLHMXIDGLKYETFYLKGCMNINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4T9</accession>
    <name>A0A1E5R4T9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">EST/SMG-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3565</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4T9">
        <property type="interactions" value="142"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4T9"/>
    <dbReference type="InParanoid" id="A0A1E5R4T9"/>
    <dbReference type="OrthoDB" id="2017974at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048018">
        <property type="term" value="F:receptor ligand activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37458">
        <property type="entry name" value="THISBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37458:SF1">
        <property type="entry name" value="THISBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13638">
        <property type="entry name" value="PIN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="2">
        <location>
            <begin position="1561"/>
            <end position="1693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="389"/>
            <end position="424"/>
        </location>
    </feature>
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        <location>
            <begin position="587"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1098"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="305"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="341"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="390"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="587"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="601"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="622"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="636"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="650"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="682"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1109"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1131"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="3">
        <location>
            <position position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ81891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1724" mass="194874" checksum="38C117C6FB0E21EE" modified="2017-01-18" version="1">MTDISNKHFTHLMGNTGVAKTNTTPTANTTSTINTSHNNNISYNNNNNTSHNNNSNNTSSNMKSVLQSGNSGGKDKIQQLQPQPQEHLYGMKENNKHLVSEINNHTVHGGESIMMSTLSNTPPASYTNVRKMGAYLNGDTNSPQLKLASAQQTSSPQLNLASVQQTSSPQLDLNNTVTNSDYIHQQNCSGIHKPHDALKNTISIAPASTTVCHSPCESIGTGAHTFVHDLETINNNNTNXNTTNDQPNNVDHYQNIDSNIDSKTDAKXDFKIDAKTDAQIDANIEKNQLGNETFIQSPKNLQFTPSSPMLPSQQQQPQKRHNSHSHEYVGNSFIKRRHYQPNPPLETPEPTPGATPDPTPKPTMNNTIDVGNEYPDTTVLSGIEKIDFVNTNNNNNNNNNNNNNNNNNNNNNNNNNNNDINNTTETRDHAHQLNIHELKSTSDKQDLQNNISAMGSPRLNDNHISSSSPSIIVANNIETESVGVIQGENQINRVSFTNTPVHAVSTLKQAIPTIASNVVPKSPTLLHKTLTHNHNHNLRPITPDITINNTNEDNVNQKSFSKSSSLSPLAKSAIVLNTHGKQLVNTTNNAHTTSIMENNDTKNTATTTTNKTAGIHQSSMDDSNRNSDGDHIMTDVNDTNEAGNNENLESGDINGNRDNGSNNSGSSSGQNNGEQGNGKDSNNNNNNNNNNNNNNNNENGSIGSINSTSKASTRQLVQKLQSIYKLIIKQENILQERCSRLTKSQGAELETLWSIYRINTYLVENYIDFLTTALSEAQLPQDLMIGKEIVEIYRIERRLWVYGCITFLDVLKNFISNFVDPQIWSQFITNVFISISEMLIELPESYRIPWFERLGDLSRMAIALYPRGFIDWKLSSEYWYNKAIMYNYGHGKLYYHMSTVEQNTLSAYVNLGKSVFCKDMFIPSQHYMQLVIDNIYQRAYSEFQNSENSIPSLDSTNNISSVNPNNGGISNYSEQLATNHHFQHHLYMSHIVEYLKHVEVISMPSFQSNPELQQVVTNYFENMFGVWLKTTTSLVPLENSDFFDEKFVFLQEGEKIKFYFRCSSLFAQSHILQMVGFGNPKNPFALLFDLPLRLKEKKDKRDKKKNGKQQQQQQQQQQQEQQQAQQDQXQPLGNDQSAMSSTTDLMSDSRMESGSVMLDDTEEGASSISSGNNLAGLKNNMLDPETFFSNLDTFNLPYEFPLNMDVWGASLSYLNKTSIWCSMTVLKKYLKGPLVCSLPHLLXWCYFLVALGKKIENLPHADLKIFWIKLIRKLFPWNTIINCLNKLILFSLKNCPDNYTDEYSRLLDMDFKDLLDYYNNEEILPEVWQCWGTLWFDTXCEKDSVPLSDNVEQFGLKNYGSVSDLSNYGLDFEPKDENGELFWYRSIRLLSLFHGISNTYSDIFGLQTLTTPHGKIGRYLFNFKEPAVLHEKSMDSDGETLSSEGSSKLMEEIESHNMNDDIASIINYYNQNVVKLLGSKGFIVDLKDFTLNGDRITCCIYTSGMLEKLNIQGADDYSITTFHNNKRLNSEGLERGEYMETVELELFRNFVKEKRLNLTDSFFILDATSWLRHSARVYKMAMNGIVKLTICLTTFQELRFLRKSKDSTVSDAATRAVIILRSLYDCGKILPLRFTGNVATSIEEHLEFEEKMTWKTHVDEFVIDAIYKSQNRLVENYADALIFLVSDDSNMRMKAINHKITSISSRFMFILCNELRPDKSVSTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4V2</accession>
    <name>A0A1E5R4V2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3616</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4V2"/>
    <dbReference type="InParanoid" id="A0A1E5R4V2"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ81946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="503" mass="58238" checksum="A105B5D9A1175444" modified="2017-01-18" version="1">MTAETPAKNLPLLIKVWTNHILPHLPLGTILNVTTLMNKETSSAFRAYEEDVWFNYIKKQHMYLLGIRTACEYYYENVKAMSWTTYARGCFSGDYSKENYAIMLGNCHATLFGSRYDHISQALVILGELKEEIGVKNYQKFFNSKTGLLKTDDVFNAFGVFLDMFDPAFSKLYTNTIAILEKITRDLKIKVEKHSISNMSHSSKCVDEVVRFLRKIMRKSNKAAGDSQFPANDLVGILRFICCPKFHKNNLSVLLVIFIELMRRLGYTVDLNKLCCLPTSYPDRYMFTFKKTANSFYGLMLHKETGLQWVLNECDQHWTFDRMIEKIEHIQNSSDCAIPNVKVPILLDLYFQNSVFMDFVLPTYHFEHVIRNNRDFIFSVKYPYGIDVVPKSIDYKIPLDLVNFNADVCPAHGCFAWSSQNMAVIVGFDTGKQKYYCLNMMNTLFLENPINLVILDNLCYPSGTLSEHRAEPKEVLHAIFKYNTQIGMFFKRYADNRFAGFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4V5</accession>
    <name>A0A1E5R4V5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">RNA polymerase II transcription factor B subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3569</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFB1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4V5">
        <property type="interactions" value="973"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4V5"/>
    <dbReference type="InParanoid" id="A0A1E5R4V5"/>
    <dbReference type="OrthoDB" id="360521at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13229">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3970.10">
        <property type="entry name" value="BSD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027079">
        <property type="entry name" value="Tfb1/GTF2H1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013876">
        <property type="entry name" value="TFIIH_BTF_p62_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12856">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08567">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BSD" evidence="8">
        <location>
            <begin position="145"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="BSD" evidence="8">
        <location>
            <begin position="223"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="372"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="386"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ81945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="589" mass="67527" checksum="9305CFD6F91A30A8" modified="2017-01-18" version="1">MSHTGAAIFKKASGMINIVEEKFPALLTWTSTDGTSTLTIELNTVDKLQATPANSDKMMLRLVGKLPENKKQKTDTDAPPQPANVKPPVTIFQFNNRIVMDGIKETLQVIIARYKDNEIYEEKMKLKENQAIQRLETPLINTSQLDDSLSFNNLLSNMELQQSLLKSNKQLFKKFQETVMNASLPPREFWSTRIPELRAFALTSSQKFGPYNVLSTIKPVASSDNKVNVRVSKEKIFTILTNYPVVRKAFDDNVPKNFSEQEFWARFFSSKLFRRLRGERIMANDRGDVILDRYLSLEQEMLQKDAMEENKHRVKRTIDLEGNEEDDPDLLGNQPDFTMRAGTDFMGNSDSTADILRRMNSLSEKMIKSLEHEYTRSTSSSKGADESDVSGDEDEEKEILIQDLEQQTKVEYAEIKIQEKRTDFERQFVENKDEKQXESVDLLLKHLNKMEKDLSKTIDLTRLCDQDGNKHNEKANLIIIDAVHVNAKQSKSFGAQEATHDSGVPMEIIEACCVLNETCCEFLKHFYNHFQSGDPRHSGITRKLHSHLEKCLLKLEIPVKENSLCEEYLKPVIKAIIRSLEKFEDVAET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4Y0</accession>
    <name>A0A1E5R4Y0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Nuclear mRNA export protein THP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3741</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4Y0">
        <property type="interactions" value="116"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4Y0"/>
    <dbReference type="InParanoid" id="A0A1E5R4Y0"/>
    <dbReference type="OrthoDB" id="5404651at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070390">
        <property type="term" value="C:transcription export complex 2"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045114">
        <property type="entry name" value="Csn12-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732:SF8">
        <property type="entry name" value="NUCLEAR MRNA EXPORT PROTEIN THP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732">
        <property type="entry name" value="UNCHARACTERIZED PROTEASOME COMPONENT REGION PCI-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ81976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="531" mass="60631" checksum="5EC36671C5F76871" modified="2017-01-18" version="1">MDLFFNQLKKPTSLSKVLSIDLTENANFIAVLQKGIINNSSLQSLFESQAKTQLEKFVDNKLASLGVDNGTRFNMLVTSYLQFVALVDPWSTWNNVDLIFDYYTNLTNLVVSLTSXSSVPYETSLCILYQKMTIYVIQLSKKIDSVNATEXTQKYLFTTQVSVLLSKYFQTIKPLVIRGSNPQIDSIYGDDEGMSNNRTTPSVSSKFKLLLWVVNRLNNLYFYIKSPKSSYTIFNNMSHKIDSRALKNLNQEVKTIFNMIDVLEYRFLLGRAYMVQNKIVDSYYQLNECFNGLLSMILYXQQANNSDISRKLVMCLNKTFKLLVPMGIAIGKTPFFIENGLNGNSHSNNNMNNFITSSIDPKLIGLYRDLGKCVLSGNMQDFSQWIAQNKTFLISQNLYLVLIEKLPLIIFRNCXKKIFTIYCFQHGSYKLAYDVLEVGLQKSLKNNSFGNFGAKTETDQILDTMFSNGIDVENLLSTLITYNWLKGNIFPMSKVLLTMKSANIKDIFPDINEKITHRFPLNEADGWISLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R561</accession>
    <name>A0A1E5R561_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Palmitoyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3831</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="10"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R561">
        <property type="interactions" value="442"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R561"/>
    <dbReference type="InParanoid" id="A0A1E5R561"/>
    <dbReference type="OrthoDB" id="302728at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF147">
        <property type="entry name" value="PALMITOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="11">
        <location>
            <begin position="112"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ82034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="337" mass="39508" checksum="CD30CE1D1610CCB0" modified="2017-01-18" version="1">MFKSIGKAFPKVLVTTLFTYTLYVTIFLRSADDGXKRFDFASGVLLVLYAISLFTYYRIIRVGPGFARDYKDALTTPYDYQTEQMYGQDHAIAVQRPKPQLLVEHSIKLKPPFCSKCQLWKPDRCHHCSSCGECVLKMDHHCPWFALCVGYKNQKFFVQFSVYTMLYAVEILVLNSVYLYHWFSDSGYTTEYIDFQVLVTWFLALIILLSMLIFNGFIFYLISENMTTIEQMERNEIKHENELVLESRGLAXNRVHPNNFYHHLQNDLNVYDLDSRWENIRSVMGTSVAEILLPITNNNVWQNRHTLNEQGLWFPVNDSYKKDIELQQRLLHRLGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R585</accession>
    <name>A0A1E5R585_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">U4/U6 small nuclear ribonucleoprotein PRP4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3511</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R585">
        <property type="interactions" value="443"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R585"/>
    <dbReference type="InParanoid" id="A0A1E5R585"/>
    <dbReference type="OrthoDB" id="540662at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030621">
        <property type="term" value="F:U4 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19846:SF0">
        <property type="entry name" value="PRE-MRNA PROCESSING FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19846">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="244"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="287"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="347"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="370"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="429"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ82059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="442" mass="49807" checksum="B5E7DB983ECCCE75" modified="2017-01-18" version="1">MTSIGDLETSLHLQNYENSKILKSEAEAIKLHLPTNEQSVDELFLALQVDRPQGLNFYEQREHLSKQIALYPAKKQQLETLLVEMKDRLNTETVSATEETDFYTPASDALVSFRLQVMAKHLELKKRVLDTRRAYVRTQSVSHQIQQRRSVLSKLDNYSLNISATGSTRPISAIKLQDQHVYFSXWNGEVKKYDIADDTVSLIYEHAGKVSALDVHKNMIVSGSTDNNIKVCTMSETGSELQIISGHLGRVSKTKWLKHFENHFISASYDKTWKLWDLNRKTDLLTQEGHDSEISSLSSNKYNSLVCSGSLDGVGLIWDVRSGSKLCQLQHTKAIHCSAWTWNDSPITGSADGSIHVWDLRKPNDPSFTIPAHTNIVTDLVLDANDQYLVSTSYDKNINVYNCGNWVKSSTLEGSLDKSLCLDVSDNQIVAGGWDRSVKIWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5F7</accession>
    <name>A0A1E5R5F7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Putative ferric reductase transmembrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">AWRI3579_g3551</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ferric reductase (FRE) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R5F7"/>
    <dbReference type="FunCoup" id="A0A1E5R5F7">
        <property type="interactions" value="399"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R5F7"/>
    <dbReference type="OrthoDB" id="4494341at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000293">
        <property type="term" value="F:ferric-chelate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF9">
        <property type="entry name" value="FERRIC REDUCTASE TRANSMEMBRANE COMPONENT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="15 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0732">Signal</keyword>
    <keyword evidence="7 18 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184613" evidence="19">
        <location>
            <begin position="23"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="163"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="225"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="287"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="20">
        <location>
            <begin position="417"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="619"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="619"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="630"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OEJ82136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="729" mass="82731" checksum="2FB8383C9DFD3774" modified="2017-01-18" version="1">MKSFKLYGIIAVLLSALQIAHAAQSFDLSWTRAYKKTLGAACKSTVTGTAMYCTKDSTKSYSCQCNDVVGMGAYVYCGYTESEGDSGSRKHFQDYFISKCPGLTRQEIEDSYNNVTKYITTTSQIKDFNASVPIRIPVKYSEATYKSAYASSYQSNINMDYCIIFGASLIGYWAAIFAVFAIYRALEEMNLSDKYVNNNRVFKFLQSQVIFKSFIGRKHKSPYKFLGYVPVTLDVIFIVGFVVLDIAGCFAGIYDLPASLKSTSSGHHGHGGSGSAKPRLIGIRTGYIANFTINLTFLFAGRNNFLLWCTGWNFQSFITYHKWVSRMTVFNVLAHAIAYYINGSNTARNLALEYYRWGIVAAIAGSAIYLQAYFPLRKYAYEIFLYVHIILALFFLVGSWYHLVRFRLQYYVFASIGAWALDRLLRIVRILNFGGVRKNNVSLINNEYLVLTVKNYNKATFKAKPGNFVFIYFGLWNCFWQSHPFTILSADSEKLKVITSIKKGCTKTVAQYLIKRNLVSMDIPLAFEGPYGTSKERIVNRDQNILMYSTNTGVAGPYHYLKSFIDKGLIQNKSIKFYWCIRNWSSVYALQEELKNLSFQSNINVIVYISQYDEAKLSSESNKSQSETDTLDHEKNNTEEKLTDCSSSNASLTMGSIQTLLPDVEFRAGRMPVYETVASDLDECLENTNVTVMTCGHSDFCDNLRDAVLKNIPKQKTKNVHYIDELQLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5Y1</accession>
    <name>A0A1E5R5Y1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">rRNA adenine N(6)-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3633</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Mitochondrial transcription factor that confers selective promoter recognition on the core subunit of the yeast mitochondrial RNA polymerase. Interacts with DNA in a non-specific manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5Y1">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R5Y1"/>
    <dbReference type="OrthoDB" id="16079at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034245">
        <property type="term" value="C:mitochondrial DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034246">
        <property type="term" value="F:mitochondrial transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006391">
        <property type="term" value="P:transcription initiation at mitochondrial promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.100">
        <property type="entry name" value="Ribosomal RNA adenine dimethylase-like, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023165">
        <property type="entry name" value="rRNA_Ade_diMease-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727:SF17">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ81943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="393" mass="45545" checksum="8792B6C33E8819ED" modified="2017-01-18" version="1">MSKIAQIAERLLQEVPSVKKLAKFTQPTDYDFLQKLYQGARLNLKEKYPNKAQTKVLDLYPGFSLSSLALYETLSPQQQLLLDTNEKFNEFVDQNLEMVKTAQQPLKSDGNKFKYYYSQLPELQNLQHAHLNPYQWATYSTITNHDISTNKTVDPERAIFLPTKQDTNKPHDEFLIHGNLTGLVCARYSGEALLMQLYGCVHRQNWMQRFGKIRMLFWVREATALKLIARPGDVGRSKASLIAETFTNTSLIAVNKATVEEPKSKRYVLHPELLAKHNPVIFSDNEAXVVTEDKERIALIEIEPAAFPSFDFEQWDYVTRQLFVLHNHPLTTSISTLGPGAKEWILPRFPKEYHDLKPTQLSREMFSEIYEIFLNWPFKPDINLDFLDIYQDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7E9</accession>
    <name>A0A1E5R7E9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytochrome B translational activator protein CBS2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3166</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7E9">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7E9"/>
    <dbReference type="InParanoid" id="A0A1E5R7E9"/>
    <dbReference type="OrthoDB" id="73846at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008677">
        <property type="term" value="F:2-dehydropantoate 2-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050838">
        <property type="entry name" value="Ketopantoate_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43765">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43765:SF4">
        <property type="entry name" value="CYTOCHROME B TRANSLATIONAL ACTIVATOR PROTEIN CBS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketopantoate reductase C-terminal" evidence="2">
        <location>
            <begin position="274"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="404" mass="45871" checksum="F2924E2E989120BE" modified="2017-01-18" version="1">MSGQVPRVYMSGSTPVLKNLALSLAQHSKQPKPVPNIILLLKDNLKLNRFLEQGSYVSSTLNAGNVQSHLRKQFMASCEPPKFANGHIAPIENLIISDFTYFDFKNNIKKYSQCLNNDERPSNVLLLNPGLDTVNYYETQWSKQNLNANLFVGTTQPGAFSKAGEFLTVKNKNILAPLTLQISQINKVIELEQENTRADEIQETSSTKEDNSQXEASTAVLSPSFQKKQEDYEETFLQLKAKNPLLDLIDATSKELPQQLIVTRKSYEHFLIEELEKVCISSCVGAMNVIFDENNMCLLKTSNNLEGIWNRMTAEFSHLVARMYPHLSYAFSNERMNNLILKYLNENKNSTLPMQQELNTHKKYNSVHRSSGLLVRMASFIKLPLPTTELIYNMAKSKINLKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7H7</accession>
    <name>A0A1E5R7H7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Genetic interactor of prohibitins 3, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Found in mitochondrial proteome protein 38</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3366</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7H7">
        <property type="interactions" value="94"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7H7"/>
    <dbReference type="InParanoid" id="A0A1E5R7H7"/>
    <dbReference type="OrthoDB" id="1696305at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050896">
        <property type="entry name" value="Mito_lipid_metab_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46434">
        <property type="entry name" value="GENETIC INTERACTOR OF PROHIBITINS 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46434:SF1">
        <property type="entry name" value="GENETIC INTERACTOR OF PROHIBITINS 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="G" evidence="4">
        <location>
            <begin position="289"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00031834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ82862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="635" mass="72644" checksum="542CBA6A1F5D1F92" modified="2017-01-18" version="1">MRKQCLKIAGFAIGPMGKFQCIDMVRTLFTPVLPRHFSSDKSPVNQNITQKHNKSDCSCVSCGIQLQNTDPDQPGFYTPPSAKTKNKNVXISDLKYLLFSQNIQKAKDLERKLYDPEFNESDDLKYSKLLSPEMIDKERNNRLSCKRCINIIHKNEYNLEKDFVKLDYDDIKKAYLSEDSKXISKHVIHVTPLPFFPIGLKKTIVADYHKVGENKENTQNELSVLFSKADQVLGSESLLNKKAPPFMDLFFEKYLDVKKGSLNVVFFSALKRWNVEAAYNILKKQFNYLLGEVNAGKSTLINAWLKKYPNSTRAELTNKNESFHSQKAGVSHIPNMTRDMSSYLLNGGSKLVIDLPGFESYTHKPPLQHLVDKKFSDLLLKTEKFKNXKVTKRPYISVKGKNEANVLGRAYTIGGLFYVIPPPRTISKVHQFIPGPDYIFSNLEKAFETVRRLKVEKDPKMIPMETYIHCKDTLAVQENFTRHIIPPFVGSVEVVIDGVGYFQLXASGTYHYYGLYEIWVPKGIKVAVRVPLQKVIEQSYDSFITAKNNCTSSSMKKDIKYGDFFPKGPLVSDTYPLEHSIPREEALAEIRKQFLERTASDVMKRGPLISTPEKELELLLTVSGKDTKLYWYYGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7X1</accession>
    <name>A0A1E5R7X1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Vacuolar protein sorting-associated protein 64</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3168</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7X1">
        <property type="interactions" value="111"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7X1"/>
    <dbReference type="InParanoid" id="A0A1E5R7X1"/>
    <dbReference type="OrthoDB" id="687730at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051647">
        <property type="entry name" value="Mediator_comp_sub12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007:SF12">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="779"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="4">
        <location>
            <begin position="273"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="275"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="621"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="731"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="551"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="75"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="282"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="311"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="621"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="651"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="741"/>
            <end position="751"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ83002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="801" mass="89937" checksum="ADACA478915AE4F4" modified="2017-01-18" version="1">MGSRNSNGDTGNLSGKSDRNSGTKIHGFHGGFHGSMHMQTNKTKNNDSSSTETGRSVNGFHSKPKKLKDDIENAQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQEQQQQQQQQQQQQQEQQQQLQQEQQQEDQNRQHVGSFLSDDTSNGNNIDHNGQTKDVGNISLLEETSFAGENDQSKSLQAPTKHLSPSLVKYTKKVSPVDAGYVPEPVIKQNGVYEAKSTNEIKDYKESGYPEKLVKPRLRLIHVLVLKSLNKTFETKYLVVPFKPEALKLGRPVSTNSGNSNANNNINGNNNNGNNNNNNNNNNDKANKSEARPDNGHFDSRILSRNHASISCDPLTSNIYLKDLHSSNGTYLNGEKLKPEIEVEIKIGDTITLGADISSNGEHKKISALVEDINILPLVDDDYTANNNTSNNINNNNTNNKKYGLNSTSVTQKAAFEAAMFGDVNDEIVSDDMWGQDNELLSGLFFNTSMGTSPSFVNVIKILNEEIMLEKAEQAKLQSMEQFLINYNANFDYLSKLRIEKNESILSQYQSNLKAQLTASYDTLLKEETDKHTFLQNENQRLKXKLKNWESEEIKNLKFKIQDLNTMLQVEKYKHGELSTSKDYKSYDTNNCDKSIGSMSSSPTHLDGLDKHNGDIFETNRNSKKINDNSKKSGNDPTDSEKINESLKGSKKGLIEGSGILTNSANGFEQIKHPMLARFQEERCGEAIQDGDDLTKTSSLQKHYFDDDNVSSNEPSEESGQEDDSCERRLQEAPSIGNQVLQNANKDKHMHTFAYLASISVVGAFTAFLAYSQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R817</accession>
    <name>A0A1E5R817_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GLC7-interacting protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R817"/>
    <dbReference type="OrthoDB" id="3972676at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="11"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="688"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="749"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="73"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="125"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="227"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="342"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="449"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="461"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="755"/>
            <end position="819"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="819" mass="88806" checksum="9CEBC1CE0002DB7C" modified="2017-01-18" version="1">MASILPILHKKQNNNNNNNNNNNNTSSNAQNTPTRQRSSSITLSNIPNGRVLVNARNANNMNDGDSILKKDNSGSASNSTRSSTRSSSSSSSNNNNNNNNRQTTLPAVSTRRKLFASLFGGSRMSTGANKGGSSSTSGKGSTGSTNTSKGNTATTKITETSTNRRSHKQLTPINTTRPEGKEKDKRHQMKRTNSASSTKSISIGQLQGTTYKGFTSNTSGISNASGTSTTDYTSYNSDIDYRSDSNTSYSNSTFNYADSVEEGSSLIKSKGSRSVSEKEPLPQDVKLAKLTPISFKRVKFQVDKFGLDPPQQIPSRKPKVCSLLLPYELISPIDIDAGISVTTATPNPTQTGTPNSTSSASGNKLTKDSREYKILLDQYNASLNDSMKFQQESYFRAKKIEEELKKLNAPTSVPKPATTITPNSTPKKPQVQAPKTLASTIKSVLPQGTPVQRDNKSNLITPTTPTTTSTTPTATPHLGTGPTTPVTPVSASSMPEMETRHSEENIIIDKPIHRHHSFVDELPTQVQTPEEPSAGNNAGEVVDTATKQEEDSLLTLDKVYTRCCHLREILPIPITLKQLKRKSSSPQNRLPVLKFLNPKPTLIDILSFTDFTSIVSNLDSLVFDNVTLNNHMVKIILLSLPKTLHKLSLKNVPLGKENWMILCKYVMTHPDLHTLDISQTKIRTNPITQAQQHDEQHLKPTAGSIGAGSTATKEESYLRVDLDWELFWRVNQQRETPVELIYVNTVTPGAPYSTPPTTNVSPSSXASPNTTTDTTPSSSKASPNTTTDTTPPSSKASPNTTTDTTPPSSNVSSNTTVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R821</accession>
    <name>A0A1E5R821_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">4-hydroxy-3-methoxy-5-polyprenylbenzoate decarboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">COQ4</name>
        <name evidence="8" type="ORF">AWRI3579_g3170</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Lyase that catalyzes the C1-decarboxylation of 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoic acid into 2-methoxy-6-(all-trans-polyprenyl)phenol during ubiquinone biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoate + H(+) = a 2-methoxy-6-(all-trans-polyprenyl)phenol + CO2</text>
            <dbReference type="Rhea" id="RHEA:81179"/>
            <dbReference type="Rhea" id="RHEA-COMP:9551"/>
            <dbReference type="Rhea" id="RHEA-COMP:10931"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:62731"/>
            <dbReference type="ChEBI" id="CHEBI:84443"/>
            <dbReference type="EC" id="4.1.1.130"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion inner membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
            <orientation evidence="7">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the COQ4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R821"/>
    <dbReference type="FunCoup" id="A0A1E5R821">
        <property type="interactions" value="523"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R821"/>
    <dbReference type="InParanoid" id="A0A1E5R821"/>
    <dbReference type="OrthoDB" id="4249at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120539">
        <property type="term" value="F:4-hydroxy-3-methoxy-5-polyprenylbenzoate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03111">
        <property type="entry name" value="Coq4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007715">
        <property type="entry name" value="Coq4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027540">
        <property type="entry name" value="Coq4_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12922">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12922:SF7">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ4 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05019">
        <property type="entry name" value="Coq4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2 7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0830">Ubiquinone</keyword>
    <keyword evidence="1 7" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00081568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ83059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="319" mass="36878" checksum="802CBDF3270A60A5" modified="2017-01-18" version="1">MLKTSASRNLCQLYSPKRPFVTTSLVTGVLSHFFLGKDAKGADKIGANDGTNEGGKNWSELVLFDKRPMEPQYKGHIPLYPHEKLLLFIASSLKSYYHPEDGINIVQLGEATAFPWWLESLKQTMLKDDVGRTILREKPNISLETLESFRYLARDPKYSKTLGATYFKWLDKEGVGPETRAPVKYIDNPDHAYIFKRYRQCHDFYHSINDLPINIEGEITVKCLEACNIGVPMAALGALFAPLRLSKYQRARLYDVYLPWATKTGLKSNPLINVYWEKIMTKDIDEVRTMLNISKPPDLRQLKKDQQRMRRELRTRYDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R9Y0</accession>
    <name>A0A1E5R9Y0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">AN1-type zinc finger protein TMC1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2538</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R9Y0"/>
    <dbReference type="STRING" id="56408.A0A1E5R9Y0"/>
    <dbReference type="InParanoid" id="A0A1E5R9Y0"/>
    <dbReference type="OrthoDB" id="3973191at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="AN1-type" evidence="6">
        <location>
            <begin position="169"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="9"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ83719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="238" mass="25532" checksum="A8FE24C7B2F407F6" modified="2017-01-18" version="1">MSEFEKKTNTNTASPTTTTTAAHTMTATTTTTTTNNNNNNNNNNNNNNNTSTSTPTSSSTDATGLSSPVPRETNSILQKDCSSHSSADSLLGSNFDVASGNVSPVHSLLIPKRSGSVSPTSLRKPIKVTKNSSTNSNLIVSPTAVATTTTAMNITVAHTTSGKDGKKQKKKKNRCALDNCNNVANKLVGHCQFCDYSFCSSHRLLENHKCAELQTCKQQLHKRNADKLNREQTIVSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA09</accession>
    <name>A0A1E5RA09_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CDP-alcohol phosphatidyltransferase class-I family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2537</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA09">
        <property type="interactions" value="264"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA09"/>
    <dbReference type="InParanoid" id="A0A1E5RA09"/>
    <dbReference type="OrthoDB" id="10251048at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006353">
        <property type="entry name" value="HAD-SF_hydro_IIA_CECR5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01456">
        <property type="entry name" value="CECR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF93">
        <property type="entry name" value="FI11325P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ83724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="347" mass="39276" checksum="B5AACFF85E6B7CD2" modified="2017-01-18" version="1">MYGISKRLFSTSSRLLFTKPVNKAFVFDIDGVIVRGATPIPRALETLQFLEKXEVPFCLLTNGGGYRELDRLKAVYDVIGFKPKNPNAFENKIVLSHTPFKEYVHQYKKVLVVGPSDSTKQVAQEYGFENVYNVKDIVQYNSSITPFSALPGLANFPEQYVKQLRTQDLDQTPFDAIFVFTDPKDWGADVQVINDLLNSDNGMLNTKRSSLQMSDKPSVPIFWAQKDLYWSNAYSLKRYGLGMFREIIKQTYKIDNNGAILQDKIFGKPMPIAYDYATKILPLKNKTKSNIYMVGDNPASDIIGAHEYGWNTCLVNTGVYSKGDPLLCKPTHIVKDLYEAVMKGLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAD7</accession>
    <name>A0A1E5RAD7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">V-type proton ATPase subunit C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2499</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity.</text>
    </comment>
    <comment type="function">
        <text evidence="5">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments. Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity. Reversibly leaves the enzyme after glucose depletion, causing the catalytic subcomplex V1 to detach from the V0 section.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the V-ATPase C subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAD7">
        <property type="interactions" value="419"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAD7"/>
    <dbReference type="InParanoid" id="A0A1E5RAD7"/>
    <dbReference type="OrthoDB" id="6605928at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14785">
        <property type="entry name" value="V-ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000002">
        <property type="entry name" value="V-type proton ATPase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1460.10">
        <property type="entry name" value="subunit c (vma5p) of the yeast v-atpase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1180">
        <property type="entry name" value="Vacuolar atp synthase subunit c, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004907">
        <property type="entry name" value="ATPase_V1-cplx_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036132">
        <property type="entry name" value="Vac_ATP_synth_c_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10137">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10137:SF0">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03223">
        <property type="entry name" value="V-ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118203">
        <property type="entry name" value="Vacuolar ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="264"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00053565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ83862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="393" mass="44816" checksum="5276C7B141D02581" modified="2017-01-18" version="1">MSELSLYTANDYIILSLPKSAQPASDPTIDVDFYLKNLLLDGKTYVSNFTIPEFKIGSLDSLVVQSEELSKIDNNLQQSITKIVDVLTNLNEANSNNYKTLPIDKSSLPEYLSDFHWNTRKFKLDKPVQNLINEITEESLQLDNDVKATSTNYINAKQQLVAAERKQTGDLSVRSLHDIVKADDFILDSEYLTTVLVAVPHSFEKQFLNTYETLADNVVPRSASALSSDSEYTLYNVHXFKKSVHKFVSMCRENKFVPREFHYSEQLIDDLKKEHDQAASLENNLRKELVRLAKAAYSDVFMNWFHIKALRVFVESVLRYGLPPQFNTKILAVPPKTLSKAKTELINNFGYLGGSAFVKDKKGNVKQNDSSLHEYASLVDTDYEPFVMYFVNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAF9</accession>
    <name>A0A1E5RAF9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Peroxisomal membrane protein PEX13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Peroxin-13</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g2967</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="13">Interacts (via SH3 domain) with PEX14 (via SH3-binding motif); forming the PEX13-PEX14 docking complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxin-13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAF9">
        <property type="interactions" value="175"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAF9"/>
    <dbReference type="InParanoid" id="A0A1E5RAF9"/>
    <dbReference type="OrthoDB" id="10037838at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11771">
        <property type="entry name" value="SH3_Pex13p_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000128">
        <property type="entry name" value="Peroxisomal membrane protein (Pex13)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007223">
        <property type="entry name" value="Peroxin-13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035463">
        <property type="entry name" value="Pex13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19332">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19332:SF1">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04088">
        <property type="entry name" value="Peroxin-13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="352"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="50"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="99"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="18">
        <location>
            <position position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00065871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ83900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="423" mass="46275" checksum="D3855F098F10CDA7" modified="2017-01-18" version="1">MLSFGYQDKQKQLIDKTQISKPQNTKAQTPXILMSSPTLKPKPKPWEVNPPTSSEXIPSVSSPSETNDPGSNGGNNTPSTSSSHXRNPSSKPALPNKPEFSTTDSSSPLNTGYNRNMGYGTGMYGSGMNGSGMYGSGMYGSGMYGSGMYGSGMYGSGMYGSGMYGSGMYGSGMYGSGMNGSGINGILPEGTQSAFQLIEAMIYTFTGFAQMLEATYMATHSSFFTMVSVAEQLQNFKEMIHGFVKGGVLDVIRLLKRLLYYASGGKLCKSSGSTGWGNNSFDNHEQKKKKEKKGKEKSKMALKPLVVFLAIAIGFPYLLNKYVTRMSKDNNNNKLNYLDKNNDHQQDMVDPNNLEFAKALYDYVPENPDYEASFTKNEFMAILSKKDALGNDSEWWKVRTRKGVVGYVPSNYIEVIKKSETNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAH4</accession>
    <name>A0A1E5RAH4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription elongation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g2565</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TFS-II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAH4">
        <property type="interactions" value="959"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAH4"/>
    <dbReference type="InParanoid" id="A0A1E5RAH4"/>
    <dbReference type="OrthoDB" id="44867at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001139">
        <property type="term" value="F:RNA polymerase II complex recruiting activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031440">
        <property type="term" value="P:regulation of mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00183">
        <property type="entry name" value="TFIIS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13749">
        <property type="entry name" value="Zn-ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000001">
        <property type="entry name" value="Probable Transcription elongation factor S-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.30">
        <property type="entry name" value="Transcription elongation factor S-II, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035100">
        <property type="entry name" value="TF_IIS-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003617">
        <property type="entry name" value="TFIIS/CRSP70_N_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003618">
        <property type="entry name" value="TFIIS_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036575">
        <property type="entry name" value="TFIIS_cen_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006289">
        <property type="entry name" value="TFSII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01385">
        <property type="entry name" value="TFSII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477:SF0">
        <property type="entry name" value="IP08861P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477">
        <property type="entry name" value="TRANSCRIPTION FACTOR S-II ZINC FINGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07500">
        <property type="entry name" value="TFIIS_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006704">
        <property type="entry name" value="TF_IIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00510">
        <property type="entry name" value="TFS2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00509">
        <property type="entry name" value="TFS2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46942">
        <property type="entry name" value="Elongation factor TFIIS domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51321">
        <property type="entry name" value="TFIIS_CENTRAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00466">
        <property type="entry name" value="ZF_TFIIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="11">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS central" evidence="12">
        <location>
            <begin position="134"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS-type" evidence="10">
        <location>
            <begin position="251"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="82"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ83871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="293" mass="33278" checksum="EDA99BA37036CF98" modified="2017-01-18" version="1">MDSKTVLQYAKDLEAMKNQEDKVLPLLTTLEKDFGQPTEKILRETKIGVVVNGFKKSTNPEIANKAKRIIIAWKEAITKQKKSKVKASSGAPXSGSSTSTKKTQQQSNDEDSMPEKPRSSKIDGVNTTVHNVKLRDMVIKAVYDALCKESRKSSDDILKISVGIEDALNKYSDKDEKKYKDKYRVVFSNIISKTNRDLKKKILNQDISAEYLVNCDPMDLAPEHLQKKLEEIKKKNLHDAQGATIERSVTDRFQCGKCKERKVSYYQLQTRSADEPLTTFCTCENCGNRWKFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAT9</accession>
    <name>A0A1E5RAT9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein OPY2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2811</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAT9"/>
    <dbReference type="InParanoid" id="A0A1E5RAT9"/>
    <dbReference type="OrthoDB" id="2402916at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018571">
        <property type="entry name" value="Membrane_anchor_Opy2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35711:SF1">
        <property type="entry name" value="ECTODERMAL, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35711">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09463">
        <property type="entry name" value="Opy2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="152"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane anchor Opy2 N-terminal" evidence="3">
        <location>
            <begin position="94"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="429"/>
            <end position="524"/>
        </location>
    </feature>
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        <location>
            <begin position="540"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="616"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="337"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="394"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="439"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="478"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="616"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="657"/>
            <end position="711"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ84025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="711" mass="77239" checksum="36006A4A994AF64E" modified="2017-01-18" version="1">MDEIEQSTTVVIPASKTMEYGFMSAWIFGSDGSPSAQNKHGDEDQLEELDDLSDEEEKVYRKLRKRATGTSSLASSSTKIFSSATATATGTNGCVECPTVECPECGSNYYCVLTSLTCTQCPLTYCALKPSTTDSSSSASASDSNHNGKKNVGAIAGGVVGGVGGAIVIFLXLYFFYYKKYYSKNRLVHAGDGSYVDKHFADDDDDDYENGEKRNFNKNSEYDGSVAAHVGSSAPASSIAQGNDYNSSQSVLGMGYNNNNNNNNNNNNNNNNNNNNNNNQNFRPPTRQAMAARNSRKPKGINLMLSDETSSISTTAASNILPIAYIPGVKVSTSTNNLRNKNINGSNNNNNNNNNNNNNNNNNNNSNNDSNGVNDDTPNVNINNGLSGFVLNSKRNKHKNTGMNKNKNRYRNKKMDDDAKSHITLGSSILGDMDDDMSVTDNNNNSTDTPFSNDDKTKKAGSNNPLARKTMMSALSEKDEEYTEDEDVTEEEKKTHGVGHNHHVPDDESSTAGESSNGTVTSSSIGSLIDISENQTAIRARPNLVQIQEEDEEDKGEEXPYRDSNLGNNERNVNSDDGGLDPGVSFATLNKIGKTHPQLKTHLNSGSVIPHTIIEEESEIPENEDGFNDDDDDDDDDDDDDDDDDSLDIGLDVQGFNKANNLHHSNISSEPETHTNNSNTAETYSEGNITNSNTLSIPDSPSPFNDKYQIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAW5</accession>
    <name>A0A1E5RAW5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Aromatic/aminoadipate aminotransferase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2610</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAW5"/>
    <dbReference type="InParanoid" id="A0A1E5RAW5"/>
    <dbReference type="OrthoDB" id="691673at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047536">
        <property type="term" value="F:2-aminoadipate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008793">
        <property type="term" value="F:aromatic-amino-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009074">
        <property type="term" value="P:aromatic amino acid family catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050859">
        <property type="entry name" value="Class-I_PLP-dep_aminotransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790:SF21">
        <property type="entry name" value="AROMATIC_AMINOADIPATE AMINOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="6">
        <location>
            <begin position="93"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ84044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="484" mass="55124" checksum="80C7E7D81A6023BE" modified="2017-01-18" version="1">MTTSVDLSFLYSEEAKKRTSSPIKTTMKYFQDPDMIFLGAGMPPAELFPVQSLTFETHNIKNLKGETSDQPLVGTIPRDPEENSIDVPIARALQYGNSRGQVELLTYLKEHTLKFHKVLYDDWDIITTTGSTNAWDSTLRIFCNKGDTILLEEYTYSSSYEAATAQGINCVPVKMDTDGIIVQDLENLLDNWNVLYPSLPKPKLLYTIPTGQNPTGISLADFRKPLIYNAIQKHNMLLIEDEPYYFLQMPKYNIGEENPLEVFPTRQSFLDRLCGSFLSLDTDGRVIRMESVSKTFAPGCRLGWIIGPXYILDTYWNLHEVSIQSTCGFAQAIISGILNRWGQDGYVDWLIKMRLEYTQKRNWCLDNLFKYMPKRKDIIRIAQPPVAGMFFTLYIDISKHPRFKSGEIKTTSEFEQYLYQVFVNNGLLLALGSWFKVADPMASASTGQLSNLVMFRGTYASITPTKMENGMRILGETIIKEFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB10</accession>
    <name>A0A1E5RB10_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Serine/threonine-protein phosphatase 4 regulatory subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3087</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPP4R2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB10"/>
    <dbReference type="InParanoid" id="A0A1E5RB10"/>
    <dbReference type="OrthoDB" id="341898at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030289">
        <property type="term" value="C:protein phosphatase 4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015267">
        <property type="entry name" value="PPP4R2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09184">
        <property type="entry name" value="PPP4R2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="155"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="260"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="265"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="313"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="333"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="369" mass="43388" checksum="3192C0F61A11C705" modified="2017-01-18" version="1">MCPLGFENEELYETMTDIITSHRDGKLNKINDIFHSAHTIHLFSTQLINRIXSEIPTLFNSNTTSEYCVVETPXIKTYLNHSPTTDYKKRLEWACEHLQKRFFSNNKLPFTMIRVIELAYHPFTYFKSHEFPKFVRSLEKCLYVTISLQQPKNMYSSMQQQQQQKQQQQNISSKSKQIDSLENYVGQKRKRTDDEXEESGENVKKQSLERENNIDEQERIGTPATPPRSITPSLQKIPWISPNQVQKAGPFLKELESIMAINFGYDEDDDDDDDDDDDEEVDEEEEQDEENDYEYENDNSQVSTTKKQHEKKFKSDSLEMKLKRDIKSNGDVIVEEYEEMEFDEDWSPSSQEDENSEEHTSSSSEEEEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB75</accession>
    <name>A0A1E5RB75_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcription factor tau subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2709</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB75">
        <property type="interactions" value="241"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB75"/>
    <dbReference type="InParanoid" id="A0A1E5RB75"/>
    <dbReference type="OrthoDB" id="4703at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017956">
        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00929">
        <property type="entry name" value="ATHOOK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00384">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="761" mass="85555" checksum="FE8DDC941D21B341" modified="2017-01-18" version="1">MPPKRKANASSKSKRTEIQEIPNKGVDNALDAKNTESKXREXRKKKLEEKDGETETNNANETIKNTKNVSVDRDQNLSLXQSIPLKRGRGRPRKSPDTVEALKSSKKHKTTKSDSITINNENEVNKDNEAVEDDDYVDAEQHSEQKKVVLRKGRGRPRKNPNDSLASKTSKSLKLSKATKTAAKNNLPKEPKMTKKRIALLAKMDDTLQNYEDVEMFYEKLHPNSINDKETAEKQMSLLGRNVLRMEKMFGLFNKEKLLSVSKILNKFDDSFLTFQNNDVLKPPEPASTTKQKNLNCFARLKKKLANNQQINXITENQFAEKFNPKDQEPRKIIFDDPNKIRSLKPGSYVDFPQFAFGNRIGKLYNTNGLVTDMVWCPEVDNGLDSKIQYLXIAKSQYNNSAIDEHLAVIRTGQPHFACIELLEINNQTQGNVEIRVIATFLHEFGELHNLKVLPVHTGNSIPLLCCTQYGTVELFNLNVEHINSQKKPLFMQLVNSVMSFKLKAEHILSFDIINQKNIIVGLESGCVAEFDLCEPDEALLLTPSYLTKIQDSHIYCVCAAHMVDETTVVATGSADGTIKAFDPKDIKNSLMTLKKSRTLSSCEIGYAPSCHAFVFSDGSGTSKIAAATTLSSASYLTMHDGYVSSVSSSKYHPFTLTGGDDGNLYICNSMKKLTVSPRTMSIFYRACRLWNWKYQKETNVYRLDGNYQVMNFAANSVTKEERTKPPGINISHCKWNETASGINIFAFVNNAGLLVVEVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB93</accession>
    <name>A0A1E5RB93_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Deoxyhypusine hydroxylase</fullName>
            <shortName evidence="9">DOHH</shortName>
            <ecNumber evidence="9">1.14.99.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Deoxyhypusine dioxygenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Deoxyhypusine monooxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LIA1</name>
        <name evidence="10" type="ORF">AWRI3579_g2795</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>[eIF5A protein]-deoxyhypusine + AH2 + O2 = [eIF5A protein]-hypusine + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:14101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10144"/>
            <dbReference type="Rhea" id="RHEA-COMP:12592"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:82657"/>
            <dbReference type="ChEBI" id="CHEBI:91175"/>
            <dbReference type="EC" id="1.14.99.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="9">Binds 2 Fe(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Protein modification; eIF5A hypusination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the deoxyhypusine hydroxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.29" evidence="9"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RB93"/>
    <dbReference type="FunCoup" id="A0A1E5RB93">
        <property type="interactions" value="922"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB93"/>
    <dbReference type="InParanoid" id="A0A1E5RB93"/>
    <dbReference type="OrthoDB" id="421002at2759"/>
    <dbReference type="UniPathway" id="UPA00354"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019135">
        <property type="term" value="F:deoxyhypusine monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000099">
        <property type="entry name" value="Deoxyhypusine hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03101">
        <property type="entry name" value="Deoxyhypusine_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027517">
        <property type="entry name" value="Deoxyhypusine_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004155">
        <property type="entry name" value="PBS_lyase_HEAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697:SF5">
        <property type="entry name" value="DEOXYHYPUSINE HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697">
        <property type="entry name" value="PBS LYASE HEAT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00567">
        <property type="entry name" value="EZ_HEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0386">Hypusine biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ84168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="324" mass="36454" checksum="2A38729329DA4484" modified="2017-01-18" version="1">MSTNFEKHFEEPTAETETLEQLRDVLVNTSKTEKLANRFRALFNLKSYAENWATDSETANRAIEYIGEGFVDEPSELLKHEVAYVMGQCKNLHACPLLRQCMLDPAQQTMVRHEAAEALGALGDQNSLDSLNRCLREDPDEAVRQTCELAIERIKWSKSGLSKEEILQQSLYSSIDPAPPLSLKEEMSVEDLQKMLNDQSLPIFKRYRAMFRLRDIGTNEACLALASGFDDPSALLKHEIAYVFGQIGNPVVVPVLIDVLGRAHEAPMVRHEAAEALGSIATDDVLPILKEHLQDDEDVVRESAIVALDMYDYENSNDLEYIPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBG1</accession>
    <name>A0A1E5RBG1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">60S ribosomal protein L35-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2898</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes). uL29 is associated with the polypeptide exit tunnel.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBG1">
        <property type="interactions" value="941"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBG1"/>
    <dbReference type="InParanoid" id="A0A1E5RBG1"/>
    <dbReference type="OrthoDB" id="528635at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00427">
        <property type="entry name" value="Ribosomal_L29_HIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.310:FF:000002">
        <property type="entry name" value="60S ribosomal protein L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3450:FF:000001">
        <property type="entry name" value="60S ribosomal protein L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
        <property type="entry name" value="Ribosomal_uL29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_uL29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018254">
        <property type="entry name" value="Ribosomal_uL29_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045059">
        <property type="entry name" value="Ribosomal_uL29_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036049">
        <property type="entry name" value="Ribosomal_uL29_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00012">
        <property type="entry name" value="L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45722:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL29-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46561">
        <property type="entry name" value="Ribosomal protein L29 (L29p)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00579">
        <property type="entry name" value="RIBOSOMAL_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00064105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ84237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="127" mass="14677" checksum="5239178205C310E9" modified="2017-01-18" version="1">MXPXIKARAGVKAYELKTQSKEQLENRLVELKKELADLKVQKLSKPSLPKINDVRKSIARVLTVINLNQRQAVAQLYNGKKYQPKSLRAKKTRALRRALTKFEASQITEKERKRQIAFPQRKFAIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBI0</accession>
    <name>A0A1E5RBI0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hypoxanthine-guanine phosphoribosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2642</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBI0"/>
    <dbReference type="FunCoup" id="A0A1E5RBI0">
        <property type="interactions" value="116"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBI0"/>
    <dbReference type="InParanoid" id="A0A1E5RBI0"/>
    <dbReference type="OrthoDB" id="9973266at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004422">
        <property type="term" value="F:hypoxanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032263">
        <property type="term" value="P:GMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046100">
        <property type="term" value="P:hypoxanthine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032265">
        <property type="term" value="P:XMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000033">
        <property type="entry name" value="Xanthine phosphoribosyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363">
        <property type="entry name" value="HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363:SF1">
        <property type="entry name" value="HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="3">
        <location>
            <begin position="10"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ84252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="225" mass="25654" checksum="182B7F49EBE7E9D7" modified="2017-01-18" version="1">MSEVEKQYISYDNVHQLCQEAAERIKLFKPDHIIAIGGGGFIPARILRTFLKEPGIPTIRIFAIILSLYEDLHTVGSETEKVGVTVQRTQWIDYEQCKLDLVGKRILIVDEVDDTRTTLHYALSELEKDAAEQAAAKGVDFTKNPELKTEFGMFVLHDKLKPKKADLPKELLEDENKYIAARRVPDCWLAYPWESTDIVHHTKMAKLQGNDIFLPEGAYDAARKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBK4</accession>
    <name>A0A1E5RBK4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Mitochondrial inner membrane i-AAA protease supercomplex subunit YME1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g3023</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBK4">
        <property type="interactions" value="1110"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBK4"/>
    <dbReference type="InParanoid" id="A0A1E5RBK4"/>
    <dbReference type="OrthoDB" id="1413014at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000175">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FTSH 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048438">
        <property type="entry name" value="Yme1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076:SF97">
        <property type="entry name" value="ATP-DEPENDENT ZINC METALLOPROTEASE YME1L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076">
        <property type="entry name" value="METALLOPROTEASE M41 FTSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21232">
        <property type="entry name" value="Yme1-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 16" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="408"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="255"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="817"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="146"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="258"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ84277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="838" mass="91543" checksum="F79E1F7DF0D05834" modified="2017-01-18" version="1">MSVSVLVPIPAVSLKCVNTSLILGLQRSLGSIAFKGRVASISNYNTTXAYKTKNHNHNIKRLLPVVANXLENTILNKNNSFLXCKRSFHSFNNRSFYSFNSGNPQSSLSLLNRKTFKYSYSTGKPPTNESNNDSVKKETNANANANASANASANINASSSGSGANADANADGPVSHDVLAQKEQEANRNLNDANIQAEFYKKLLKSGYAQYVVSRYETPGIAQSPECMSLYMDALTRTGKHNQADAVRQVINTSSTANGVNNPTMNNSNGNGNGNGMNNTTNMNNANTSGMNPPMNANYPYMNPFFGTPKEPIHVVISESTATIVLRWIKWLIIFGLLTYGFSEGFKYLSENTSIFKNSEVSDKSVDVAKANIKFEDVCGVDEARHELEEIVDFLKDPTKYETLGGKLPKGVLLTGPPGTGKTLLARATAGEAGVDFFFMSGSEFDEVYVGVGAKRIRDLFKQARSRAPAIVFIDELDAIGGKRNPKDQAYAKQTLNQLLVELDGFEQTQGIIIIGATNFPESLDKALTRPGRFDKIVNVDLPDIRGRTEILKHHMKKVTLSKDVDPSVIARGTAGLSGAQLSSIVNQAAVYACQKNATAVDMSHLEWAKDKILLGAEKKSMVLPEVTRKATAYHEAGHAIMALFTPQTTPLYKATILPRGRALGVTFQLPTNDKYDITKSECLARLDVCMGGKVAEEFIYGKENTTSGCGSDLRQATKIARAVVTSYGMNEKIGPVELSEDWNLWSGKIKDLADQEVIGLLKNSEDRARQLLKEKEVEFRRLAEGLIEYETLNRQEIDKVVKGEALDRPKYATNKVVETPDNDGRKGIDKGSLGAVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBS8</accession>
    <name>A0A1E5RBS8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">C2H2 finger domain transcription factor mtfA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3034</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBS8"/>
    <dbReference type="InParanoid" id="A0A1E5RBS8"/>
    <dbReference type="OrthoDB" id="3973247at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000761">
        <property type="entry name" value="Zinc finger protein 449"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050329">
        <property type="entry name" value="GLI_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818:SF139">
        <property type="entry name" value="PAIR-RULE PROTEIN ODD-PAIRED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818">
        <property type="entry name" value="ZINC FINGER PROTEIN ZIC AND GLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="479"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="507"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="235"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ84341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="539" mass="60188" checksum="ABCD82A4CEF9AA4D" modified="2017-01-18" version="1">MMEQNYQSHYGNQVQRLQTPIQLDNNRIKENEMNRYITTTNSNNNICGNPNNSISISNITNNADNNTCNNMCNNYNGNTPHVATESRLLKHVSLPSLYQHLSDSKVLLNNNTNNSTNNSTNNSTNTSINTSINTSTNTSINTSINDHSGSVSGTSFNSNSYTYGDAYNKAYKNTSNSFSGITNMHTTSTPISENKNYNNQSISQISFNDYQMVEKKRKNSFLIGINNRHKGCGDISRSGNNSSGNENNWSISNNSTRPVSSKTDVSPLHVSGYEKDELRYYRPMEMAKRNSIAMLPPLTTVNGMTNGISNMSNTGNGYTTTPTNQYINKSFNTTTQEIHMNERSNTFPLLLTPNSSVGFNTPHLFHXENTNHQLTPTHAKIPFVSPHHQQHQGHEQHRGYFEQVHVPLQSRTHAPLPPNRNISITPXSSLDNSPTVSPGMVANRDLHLSAVSEFKPVHAENSCKKYKVTKDITKKGTQNVCHVCGKSFSRPSSLVTHENIHTGKKPFVCKFAGCGKKFNVRSNMNRHMKGHLTQKRVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBW4</accession>
    <name>A0A1E5RBW4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Elongation factor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2669</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBW4"/>
    <dbReference type="FunCoup" id="A0A1E5RBW4">
        <property type="interactions" value="1271"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBW4"/>
    <dbReference type="InParanoid" id="A0A1E5RBW4"/>
    <dbReference type="OrthoDB" id="364892at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01681">
        <property type="entry name" value="aeEF2_snRNP_like_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04096">
        <property type="entry name" value="eEF2_snRNP_like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01885">
        <property type="entry name" value="EF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16261">
        <property type="entry name" value="EF2_snRNP_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03700">
        <property type="entry name" value="EF2_snRNP_like_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000010">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000006">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000003">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000002">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000058">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF10">
        <property type="entry name" value="EUKARYOTIC TRANSLATION ELONGATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="9">
        <location>
            <begin position="17"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ84388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="842" mass="93377" checksum="F95B971E03EAACD8" modified="2017-01-18" version="1">MVAFTVDQIRGLMDKVTNVRNMSVIAHVDHGKSTLTDSLVQRAGIISAAKAGEARFTDTRKDEQERGITIKSTAISLFAEMTDDDCKEIKQKTEGNSFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDTVEGVCVQTETVLRQALGERIKPVMVINKVDRALLELQVSKEDLYQTFARTVESANVIISTYADEILGDVQVFPQKGTVAFGSGLHGWAFTIRQFANRYAKKFGVDREKMMDRLWGDHFFNPKTKKWTNKETDASGKPLERAFNMFVLDPIFRLFAAIMNFKKDETNTLLEKLEINLKGDERDLEGKALLKIVMRKFLPAADAMLEMIIIHLPSPVTAQAYRAEQLYEGPADDEACEGIRKCDPSAGLMLYVSKMVPTSDKGRFYAFGRVFSGTVKSGQKIRIQGPNFVPGKKEDLFLKAVQRVVLMMGRFVEPIDDCPAGNIVGLVGIDQFLLKTGTLTTYEAAHNMKVMKFSVSPVVQVAVEVKNANDLPKLVEGLKRLSKSDPCVLTYMSESGEHIVAGTGELHLEICLQDLENDHAGIPLKISPPVVAYRETVEAESSQVALSKSPNKHNRIYLKAQPIEEECSLAIEGGKINSRDDIKARARIMADEYGWDVTDARKIWCFGPDGTGPNLVVDQTKAVQYLTEIKDSVVAAFQWATKEGPIFGEQMRSVRVNILDVTLHADAIHRGGGQIIPTMRRATYAGFLLAEPRIQEPVFLVEVQCPETAIGGIYSVLNKKRGQVISEEQRPGTPLFTVKAYLPVNESFGFSGDLRQATGGQAFPQMVFDHWASLNTDPLDPTTKAGEIVLTARKRHGMKEEVPGWQEYYDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDS0</accession>
    <name>A0A1E5RDS0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UPF0507 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1812</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0507 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDS0">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDS0"/>
    <dbReference type="InParanoid" id="A0A1E5RDS0"/>
    <dbReference type="OrthoDB" id="7464126at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030133">
        <property type="term" value="C:transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097422">
        <property type="term" value="C:tubular endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045022">
        <property type="term" value="P:early endosome to late endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051248">
        <property type="entry name" value="UPF0507/Ank_repeat_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24170">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24170:SF1">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G09870)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109993">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VPS9" evidence="2">
        <location>
            <begin position="312"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="466"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1132" mass="130464" checksum="0FE40C199CD4D63E" modified="2017-01-18" version="1">MDAVANDLQSSTNDRKFATPHMLNPIVDTIFGCQDPSNFEFASTFKNIFGKNFILLVPPTELMPFVNKLALAKTKEKSKSPKYCSREFLESHILIPVSKEYVQKGKEPQAYVLLADRKNLVDISNCELRYKAQTRKILYTQYLPISNDYFHNKSHILLLHIDQCMNFEQKGSNTSVSQNLKLANKDSEQTSLQSSKHKFELVNQEIASFELLLRLNPKVQHELKNKISRECAILEHTSFHKIFDMASVYFRALNTIIEWCLNEPFFEGYKQLGRMVYKYVEAQFCNMILKKLHAFVKEPGSNICETSPEFVNLSFTDIPSFSESLQQDFIMSTRKMLALEKNLSNAIPILQKMFASKNCDTKSDYLLSALQELSRSQDADADILVGLLILCICRASPKNVYAELLYLQNFMNDTSKITFGSNAYAISTLEVAIKYLVSPENNSYLMERSVRNKEFWXILQNGTYEDFTRFIESRNVLEVDDILFGRNYACKSCFSLMVENKGDGHKMFSHIMLXFPQLKAIDITRLLDDRGANNETLLSKSVKLRNDSFTNLLVEVLLSVCTAHEIELYVNTKECDTDRTVAHYIGLDCENLLQLGHYFDWETLDNQSHTPLFALFRFYDHVDYNTLVDHGFRTCLFWYRKKQKQFXFSTHMDTKGNTLLHIAKSNVAVLLSGLPNPPVNQQNRAGLTPLMFYCRYSRVENIKTLIESGKKALLHVRSEGNQKYLGYSCFDFAKNEHVLRVLGKYLLSIKDIVGKTKILALRCEKACXYVYLVIKSSENNVEVVKHKLKTLKAWVSAYFTRHPKSFLLQSKNFMEVFPDALVPLSGIFAKFELDKKLINWGQIITAINIIQPDFLKALLSANDAHMFDQFSRSNAKYNALAQFSPSRTQKIQTTPEELYSFKSVLKVSLEECEALQHYLKAXKQLLVVKMVKRFDLEYVESLLFVXIFNKKKSQQELYCHIFNSEYFDALSISCVSFLLXCLSTIVVNLQGVLHGSFVNWWKVYGDYLQLQRKLYDVNASLSPTKHMAIARATNEDMAPTTTNNSHTVCNETSSKFSSFFDNYFEGNKRKYREKLITDINLEAQRFKAADIVLREDYKNIAEEMSKFMTMKDTFFHSYCIVETAASNTTXFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDZ5</accession>
    <name>A0A1E5RDZ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CoA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2391</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDZ5"/>
    <dbReference type="STRING" id="56408.A0A1E5RDZ5"/>
    <dbReference type="InParanoid" id="A0A1E5RDZ5"/>
    <dbReference type="OrthoDB" id="5138418at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33303:SF2">
        <property type="entry name" value="COA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33303">
        <property type="entry name" value="CYTOPLASMIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA-binding" evidence="1">
        <location>
            <begin position="13"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="174" mass="19340" checksum="F8E5A7922D3220CF" modified="2017-01-18" version="1">MSLKNFFGPKTLYFVCGKVYKDNSYANRIVHWFVQHGLPVIPITPTLGTVDLSVDYITAKRQPHLPTLKVLPSISVALEVLNKNDTNLKEKIDFVSISVNFVTPANITLQILKEIKACGAKIQACWFQPGSYDSACVNYAENELQLGKENVISDCVLVKGFANMCPSDIKPFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE18</accession>
    <name>A0A1E5RE18_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein CBP3, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1884</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CBP3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RE18"/>
    <dbReference type="FunCoup" id="A0A1E5RE18">
        <property type="interactions" value="106"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE18"/>
    <dbReference type="InParanoid" id="A0A1E5RE18"/>
    <dbReference type="OrthoDB" id="10253878at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021150">
        <property type="entry name" value="Ubiq_cyt_c_chap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007129">
        <property type="entry name" value="Ubiqinol_cyt_c_chaperone_CPB3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12184">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX ASSEMBLY FACTOR 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12184:SF1">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME-C REDUCTASE COMPLEX ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03981">
        <property type="entry name" value="Ubiq_cyt_C_chap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquinol-cytochrome c chaperone" evidence="3">
        <location>
            <begin position="145"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="37"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="334" mass="38753" checksum="8F14AC7D5E6776A4" modified="2017-01-18" version="1">MLATRSIRAITKPSLLGSLHTCSLKAQLRFYSSANNQSESPEDLVKHSELHSKPLDPSNNAPVTSKVEKSKRTLLSDSKYENPNYQLPKWKEALAEVVIKTFKMDMDKIRAGPIAGSYYYGECKKQGLQYADEELSASAEFFYKDLKLPRTFSQWFQITILHEWILFTRMRAMPFKYGRNYQQKLVDRTFSDIERRLQEEMNVNSTRITDQYLKDFNSQLRGAVFAYDEAFATDDLTLAEALWRNLFGGRKNIDLVHLEAMVRYVRSQCYVLSKMSDRDFAFGNFKFVPADEVVKPLTKEQEDQMRKATIAKYKEIDSNPNLLPSEKSKLSYEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE46</accession>
    <name>A0A1E5RE46_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coupling of ubiquitin conjugation to ER degradation protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1689</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE46">
        <property type="interactions" value="102"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE46"/>
    <dbReference type="InParanoid" id="A0A1E5RE46"/>
    <dbReference type="OrthoDB" id="3973217at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14424">
        <property type="entry name" value="CUE_Cue1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041158">
        <property type="entry name" value="Cue1_U7BR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18499">
        <property type="entry name" value="Cue1_U7BR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CUE" evidence="2">
        <location>
            <begin position="73"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="221" mass="24517" checksum="E587FE09A9BADC12" modified="2017-01-18" version="1">MDKSDITYAAGAIGLIXFLKWMFQSESKHPSLQQAQEDERGNAINNAVNTNRNNAQENGANDDGNGRRYRRAVTQDMIDVVKSIGPYLTVEQIRYDLEKTGSVEETVNTFLSTGTLPIPPNIPPRSANISNTHTGSASSSSFDSTGSNSRAGSSLSQVQDSATTLQRKCHFVPENLISKYXVDVNKDYSSVDYKTLSLDEKKQYMIWKARKGYLEALNKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE60</accession>
    <name>A0A1E5RE60_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Ribosome biogenesis protein BMS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1882</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRAFAC class translation factor GTPase superfamily. Bms1-like GTPase family. BMS1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE60">
        <property type="interactions" value="1530"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE60"/>
    <dbReference type="InParanoid" id="A0A1E5RE60"/>
    <dbReference type="OrthoDB" id="10260897at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01882">
        <property type="entry name" value="BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000105">
        <property type="entry name" value="BMS1 ribosome biogenesis factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012948">
        <property type="entry name" value="AARP2CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039761">
        <property type="entry name" value="Bms1/Tsr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037875">
        <property type="entry name" value="Bms1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007034">
        <property type="entry name" value="BMS1_TSR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030387">
        <property type="entry name" value="G_Bms1/Tsr1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858:SF2">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BMS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08142">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04950">
        <property type="entry name" value="RIBIOP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22298">
        <property type="entry name" value="Tsr1_G-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00785">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01362">
        <property type="entry name" value="DUF663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51714">
        <property type="entry name" value="G_BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Bms1-type G" evidence="12">
        <location>
            <begin position="72"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="325"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="462"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="571"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="645"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1146"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="325"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="470"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="514"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="581"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="592"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="649"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="680"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="696"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="718"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="730"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1146"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1193"/>
            <end position="1206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ85156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1206" mass="137876" checksum="7D6F44740D55BB6C" modified="2017-01-18" version="1">MGAEIQQTNREHRKKKDDSKTAKKKLHTQGHNAKAFAVSAPGKMARQMMRSGDKNEDRLHVPMVDRTPDDDPPPVIISVCGPPGTGKTTLIKSLVRRMTKTTLNDINGPVTVVSGKKRRVTFIECPSNDLNAMIDISKISDLVLLMIDGNFGFEMETMELLNLLQHHGMPKVLGVATHLDLFKKQSTLRATKKKLKHRFWTEVYQGAKLFYLSGVLNGRYPDREILNLHRFISVMKFRPLKWRNEHPYMLCDRMTDLTHPELIETQGKQIDRKVALYGYLHGTSLPSFKNFKVHLAGIGDYSISSVEKLPDPCPTPFFQQKMDEYEREKAKENPEAEGTTPKRRRRRLDDNQKLIYAPMSDVGGVLVDKDAVYIDVGGAKGKDQKQSFVEGEEAGEGEEMVVGLQKVNKTLGEQFSDVGLQIFSNSNELKNVDEEPSDLDXDEQETAIELVNTGRTKLRNPRLYGKSIQDEEEVEGEEYEDDDNENDMDVDNESLAPRLVEVDFQNNNEKVEAETXDEFSLSDDEDGSAESWELSAANKLKGTINKNKKWDISKLIYMENISPEECIKRWKRDPSQXGNEENIEDDNEDDSFFTKKGENQIKAKPEDVQDDDLEKFEPYFSTMEELINKWDNIDPIKNRFITAPKIDEDGADAANDNDESDVDGEEEAFGDFEDLEGEGQATSAAPSKVSKTAENSSDEDVEDVENENQDDFTNFDKEMEETDEEAEQDGTSKELTVEEERELNATKKEKLRLQFEMEEGDNFNEDDPDNEYDTWYELQKAKMSKQLDINNAILNTLSEDQRKNIEGYKAGSYVRIVFDNLPMEFVENFDPKTPVVMGGLLPTELKFGIMNARLRRHRWHKKILKTNDPVVLSLGWRRFQTLPVYTTSDSRTRTRMLKYTPEHAYCQASFYGPLCSPNTSFCGVQVVANSDTTGSFRISVTGTVEEIDASVEIVKKLKLVGYPTKIFKNTAFIKDMFSSAMEVARFEGAQIKTVSGIRGEIKRALSSPEGNFRATFEDKILMSDTVILRSWYPVKVKKFYNPVTSMLLKDKTEWKGMRLTGKIRYEQGLLTPVNHDSTYKKIERVDRKFSGVKVPKSLQKDLPFKSQVSQMKPQNKKTYMQKRAVVLGGEEKKARTFMQKIFTVAKQKDEKKRAKQEEYLEKKADKQQKEASLKQEKERERKRKYFSTAGKSSSRDGSESSSKRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REC2</accession>
    <name>A0A1E5REC2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1857</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REC2">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5REC2"/>
    <dbReference type="OrthoDB" id="2135762at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013950">
        <property type="entry name" value="Mis14/Nsl1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08641">
        <property type="entry name" value="Mis14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ85251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="292" mass="33971" checksum="039BF8B0CB4EA119" modified="2017-01-18" version="1">MEEQYESAQKTVLSLEDLQSIFKTFQMDLQERLELILPNTNTPSEHGQTELQENQQVQSIIEDCLFHAMYSIYESFDVQGVDTEYIGKQIAFIANEQRKALSTAVEETQAAXDEFDTGENTNANNVDFLDENTVDIRCVLLRDDEKDMEAFDEKLSDQVRSTYQDWEDQMMRIAYLRKHGYVEVTDEYTQQCDNFLTTIDQQIESCLSTLXGEEEEEEEEEKEEEEGMAKNINIGDETNGLELQSILKEYQPQYVNALAELKDSMHNKIPLIKRRSVVIDKKRDFLKRELGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REE0</accession>
    <name>A0A1E5REE0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein ECM5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1868</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REE0">
        <property type="interactions" value="353"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REE0"/>
    <dbReference type="InParanoid" id="A0A1E5REE0"/>
    <dbReference type="OrthoDB" id="1678912at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16100">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15518">
        <property type="entry name" value="PHD_Ecm5p_Lid2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013637">
        <property type="entry name" value="Lys_sp_deMease-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF113">
        <property type="entry name" value="PROTEIN JUMONJI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08429">
        <property type="entry name" value="PLU-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ARID" evidence="6">
        <location>
            <begin position="322"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="7">
        <location>
            <position position="557"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="7">
        <location>
            <position position="1130"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="7">
        <location>
            <position position="1279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1516" mass="175569" checksum="A2C25622649814A4" modified="2017-01-18" version="1">MNPIENHSDQMYIQSPNHSSMTTNMPNNTFSNYPQPNGFTQQQTNRLLPEVASQVNSSLPQIERSRYLSIHELTNNHSELPKARETLNGNAESIVNHTTKPPTLQNGSHMTNSIXLHKPVSKDPQISTFPVHNQMRLKKPKTDFPVEVAMSTQSNTSSTKYSQPKTKRYKTQLHTYKMAMAQQAGFYRCSSSKNVLSPIGMQSINKWSPRLENVYDMSSTXDGQTLEKLTDSXTGLTYMLKEGYVPFFELDENSKYIENPILFYEEIKHLGIKYGAVQVKTKNSIVKNNIVVNDINEQFKKDLDSFQFHSRHSIYNDPQVQIXIVSDFYTKLYEYYKANGMGEKFNKLPTVDKKPLDLWRLWSCVQTHGGFNNVCFRKMWLQIGKELGYSGKIMQSLATTLRSNYSKIFGDFTYSYLGQEXKGHTATTNNFAVNPSQNTKLCTLNTQHEQILLKDLKILEXHEAKSLAPPPFKKRKLVADLDENDFESYYWHEGAEEYTNDYSHKSGSTPYFTIREMVLKNNIFSEFLQNNFEKNLQVGISADELEDVFQSLLVNPDFFYGVDAGYQVPSKFKHHSSIGFWNIKKIETTEQNLQRLQPVDYNNTHGSKYDITMMLSYQDWSSRNYGFPTMEYLEYGASKLWHVVPQSEVGKFESLQAASRITDPTDQVSANHNHTDFRYSYSEQTESFQNFSAWSKPFFENCTAQEYISTKTLNELGIKNYKIVQTPGTCVFTFDHAMTSKIASGFSFSENICFATESFFDDQRLFDPLFLMNVYWNGSPQQMEWAKKHLENYKQKSSFTGPDMESAKIIPQIVQKFYYDIDFYKNXSLDDAAKDEGSLHHRIGQMLSSQPYLSENKLKHILFQTHKSAKNTRDWQKISNFLLHDLPILKHEAVKFFSTLKPITFEFKWGQNFFLKYSNGEPIDLAELGKVVQYLRNCPVKCPEFEKLYDTYNTVLQSINALDQLCISNEQKVDIVKYHDMLTECLAFGVKYDSMQQHCQFYQKIIWFEVYRKLMDSKHGTYLCEQLYQLLKLGIDYDVESKHLSVLSNLIQKTCKINERIDDLFKKRHGHCLKVSIDEVGNWLNEISKEKLPIDXLFVDLFQGILDRVQSSRLMYEPLVNKLECNSIHLEGFTKMINDMEWMEGLVNXFHKNDQRFTLDQFKKSLSDHSFSESKASGALMTLEDLFSKEIQKTEEWLRQAFRFFKSSEHLSEIIENAERNYAIESFEQLQFACRKPTVTTEGSSLKVEHXLVDIAHKEVPMAHTDDPKPEEQMKDIDLKAEESSTKNDLDAAKPQELYCYCRKGDSGSTMVACEICQEWYHVDCVTNVNPDNQANDPDPVNNVFLCSMCFSGVVNLKNYDGIKFSDFQKLMIDYSKLKIVPNETLFVSKVFLFYEMALAYRNRMVDYLFDLSASRGSNSLTTSGVSIAMAPNATKFVLKADVTIEQCKHLLRKAMGGHCYFLNEIKSIKRFIRKTDINDVNKLKRKKIDIVTGYPLSEQPFKDKLHFLSLDEHYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REH3</accession>
    <name>A0A1E5REH3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcription regulatory protein SNF12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1715</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REH3">
        <property type="interactions" value="786"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REH3"/>
    <dbReference type="InParanoid" id="A0A1E5REH3"/>
    <dbReference type="OrthoDB" id="10263741at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13844">
        <property type="entry name" value="SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="269"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="496" mass="56093" checksum="00348F24A412B0D5" modified="2017-01-18" version="1">MNASNTTYPTDSYIPPYLKELIPELGSLSSLQEKETVLDRYLVRKRLELEELAEENIVSFEEDLKSNQXLRIYVSNVAGDQPWQNPETESPNWTLRVEGRLLDSQYKADDPARPKFSSYLSSIALDFEQAQENDHYAQDPLDVVEWHENPHGPPVEFDGLDVKRAGSRNVNCRITIQPKSQTATVLQYSEPLSNITFNKNGALQDALYLVYTYITSNGYFDSNGNVLLSSDLLKNLVHYNKNKENXTXHSSGIRNNSVSFQTNGVASNTPATETEXKPATEQAPSSAQQNAAEPVDVVMEDTTTSVSEHSEDANNDQPQQQHEHGSQQEIESITLNELNTLITKDHVKPLPPLVINYTIRCDKASTYGETCFDVVVKNHQDSAKGDTQKNKYQQLLQEYMDATSSINNDIQKSDTKISELMVELNNTYHRHQFYAQLSKDPVTFLNKYIESSSNMMKVLSGDEGFQEDEVRNSDFYKKNADILFEDIAVXYANGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REQ5</accession>
    <name>A0A1E5REQ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Guanine nucleotide-exchange factor SEC12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1704</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Guanine nucleotide-exchange factor (GEF) required for the formation or budding of transport vesicles from the ER.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum membrane</location>
            <topology evidence="10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Golgi apparatus membrane</location>
            <topology evidence="10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the WD repeat SEC12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REQ5"/>
    <dbReference type="InParanoid" id="A0A1E5REQ5"/>
    <dbReference type="OrthoDB" id="2013972at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003400">
        <property type="term" value="P:regulation of COPII vesicle coating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045260">
        <property type="entry name" value="Sec12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23284">
        <property type="entry name" value="PROLACTIN REGULATORY ELEMENT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23284:SF0">
        <property type="entry name" value="PROLACTIN REGULATORY ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 10" id="KW-0813">Transport</keyword>
    <keyword evidence="2 10" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="508"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="695"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="722"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1063"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="514"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="699"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="744"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="909"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1073"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="12">
        <location>
            <position position="654"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="12">
        <location>
            <position position="902"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ85380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1090" mass="121206" checksum="64056F989B59DB57" modified="2017-01-18" version="1">MKFESTIYNVGYPIYGAKFISDRYLLTAGGGGEGANGIPNKLSVLKINFAKPQKPVKKFRELSLDDLDDSPTCLDFWLPEDINNTALGNNNSNNSNGNGFSNDNNGIVLVGCNENSSNVKRGTNRHLRKFAWENEHLRFLASCDYNKSTDPSVYTKFLSMSNDGXHAALASSELPSIIRVINPQDLSETYEIETGNEVKDLDFSPNGKVLAYITETSIDIISIVTGSFIVRKMDFDKNLALSKVKFLNEDKILVISSSASKGISLSILNVKSGSISVVKSKVLKNLKFKGVTAMAVDTKGTLLVLAGNDNSLSLIKLTKNQSDFQVMKTFKDVHSFAITKVAISPNSKIIASVSAANTVNLIKLPENMANSLSVGEKLWKFFVNFVLIVLIAALLQLGYHYNAHLFILEKTKNTIAQYRDSNDSSSYFTMSEDIMSPTILTKKTGSSSVSVKSSLSTPMSSYTTSLTTSNYRDEYYANEYSSELISPSAETAKTLGITEKSDIVSETLNSKTKSVTKQRSPRTTKSMKXKKPKTKTYKKKLTKVSKNAKQTAVSXSLETSLSHSALTTMTKKDEPLYTTAASSNVSAFVXLSSSINSLTSSPLDXSSSSQETQITKPLTASDAVTNNEEDLHSIIQNEYDXLAXERQAAVNELEEMISLNSSEIADAKDEYSDLEKDLESAAEGVEDLESELSEIENVAHTQTTPTSENKLDLSRDEVQTXXADATQVNNDXAEEPVSRPPQSVEEEYAEDAEEYEDAKEDEEYEEVEEVEDAKEDEEVEEVEDAKEDEEVGEAEDAEGAEDVEDAEEVEEVEXAEXVEDAEDAEDVEDAEDSEEVEDAKDVGEVEAAEVVEDAEVVEDAEXVEDAEDAEDSEEXEDXEXDEDAEESELENLEKIPKTQNSEDNXFDKPEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEDYAKNNIKEFSEVIEISVDNEANANVPQPEVTXTAFYKVYENVTETVEVTYPTPDPPLDEPLIAIETPLPESLEAENESTTAFSDETSIAYDEQISEPVPMDEPVETEPETEDIIEQDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RES0</accession>
    <name>A0A1E5RES0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein arginine N-methyltransferase HSL7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2284</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RES0">
        <property type="interactions" value="1095"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RES0"/>
    <dbReference type="InParanoid" id="A0A1E5RES0"/>
    <dbReference type="OrthoDB" id="3971296at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016274">
        <property type="term" value="F:protein-arginine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035075">
        <property type="entry name" value="PRMT5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035248">
        <property type="entry name" value="PRMT5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035247">
        <property type="entry name" value="PRMT5_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10738">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10738:SF0">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05185">
        <property type="entry name" value="PRMT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17286">
        <property type="entry name" value="PRMT5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17285">
        <property type="entry name" value="PRMT5_TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PRMT5 TIM barrel" evidence="7">
        <location>
            <begin position="32"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="PRMT5 arginine-N-methyltransferase" evidence="6">
        <location>
            <begin position="347"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="PRMT5 oligomerisation" evidence="8">
        <location>
            <begin position="519"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="713"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="831"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="717"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="745"/>
            <end position="762"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="896" mass="102155" checksum="304E7E0E216242BA" modified="2017-01-18" version="1">MLKSNVFVGVKPPLNQLVQYDENSKPIDEKTFDYTLLPVTNTKYRNTVKKYYMDFQDPIYMLKVPPPQLQEISISPKTQELSHVAVQNDLXXSLEPSETARSRTGQLMHIGLVSSWIELESNDVAIRELSYQVLKHEWDYAKFVGIKKLILAPPRNLQNLNEYASMISRLLNSDVVAEENVFNNNPVLSISLPIFEDTNPLSTWELWNTIRNLVDYHPNFTISLALPKYKTPSYVVERWISEPVSCLLLSSSIFSPNRFNYPVLNKYNQDIVYQFQKTNGNQQFCXDQLVIILHGLEKYSKFFVKXGGEQAYLDYTNFLLGKGDKKLAKKYENLMSFGYDANLKAFLSSSILPPLKPNSETLPNSVYHTFEKDSMKYEQYETAIFAALQDLRNIPRPAKTLKIMIAGAGRGPLVSRVFISLQKLGFAKHQYKIMAVEKNPQAVLYLQNKNYQDWNNEVQIVKDDIRNLAIPIEDDENEKFDLIVSELLGSFGCNELCPEILQFLDLYFSKTSTIFIPWKYSSYLAPVSSSLLYQTLASKDSRKLFEKPWVIHNIPYTVLSSKINEIWSFAHNGELYGNGETQIGEDGYSLTFLSRSVTTKFKMKQKCEIHGLLGVFTANLYKNSVVLSILPKKPVKFLSPTNTDLKTYQRTRSLHSWSPMIFPFKQPLYVTDDTELEVFFTRNNDGQRTWYEWSAQSFMYLITDEVPLVSNEPSISEENHQKKAEQCADDKHAEDQRNARISVGDMNSSSIPANSTSLRNASNGKYADTELNQNQNYVSADETGWESVQDIHNLLINGDSTKRNSYLHTEVSKILSKPLFDLKITXSAAXTSPAPAKNISGALKVSNPQGSPLPALDMEDNKEPENKPVEYHIRVRANGVTQHNVNGRFFSISGSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REW3</accession>
    <name>A0A1E5REW3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Outer spore wall protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REW3">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REW3"/>
    <dbReference type="InParanoid" id="A0A1E5REW3"/>
    <dbReference type="OrthoDB" id="4068630at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051402">
        <property type="entry name" value="KPR-Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708:SF34">
        <property type="entry name" value="OUTER SPORE WALL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708">
        <property type="entry name" value="PROBABLE 2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="753"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="753"/>
            <end position="762"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="776" mass="88318" checksum="436EEEE92BCE7FC4" modified="2017-01-18" version="1">MTVEPLVVLSVGETPTTQFVSWRLSLTNTFLVQVSEYIAADGVVSWKSNKYGSNFYKPNLFAKDLESLNKLVFDERGTPKYRVDILLLAANSKQQYLELTRSVKRYVSQKTLIIVDCNFGVSLDELVLQQLEGINHSGVLSLLADIECRKLSSGSYVLVSEMCKLFFGLTYSKSKFSNEQIKKLQEHNTDLIRVQYADXGSVLNFLIQAIVSTGINNVHKVSVDSNQMPVKVWYYTIPKIAFNALSIVFEDPYYELFTDPKDFPRSLSEDIIKELMVIAYEHSGEILSNFCIPPDETSETRRRXLVNSISYQKVFDVVKNKSKELNTFTISEAPEYLTLSFETYSFFHKIDYPAAVLLSHTLELAAVYKLPKLGLKFLFGFYSKLSLLAGMPLSNNAGRKTKDSFLFGRNFFNGAMSKTQSNDSSNFXDFKSSMNNQSELYAESKAKMSKHRCNCQANLTKPLNNPKVQNANSYSGGKVEXPLYSAPDDKERYVCEGLFDLYADVADYNLDHNETSLKNSRLEVENKNNIAEVQSDYSSDDGYYSLHDYYQNTDMNNKKFQKSTSTSLAPYKKKLATPDRSEKQKEPVFFGFYLHKDPYTKEVIERYLTDVDGDDLEKALRNCHLYDDFGRYHNNLPCKNGSLANATSVVRHDASKAKMLVKMTKLCKKKAAHSHRNLSTSIKEAELSYLQGIKMSSQDPVNSGNFGHLINVSSTRFGNLEFAYQLIQKNEKQRLKCKKDLMSNRYANIKKDFKPSKQKLIEGPDSQDQGLKKNTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REY8</accession>
    <name>A0A1E5REY8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">60S acidic ribosomal protein P2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2254</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes). The 5 acidic ribosomal P-proteins form the stalk structure of the 60S subunit. They are organized as a pentameric complex in which uL10/P0 interacts with 2 heterodimers, P1A-P2B and P1B-P2A.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REY8"/>
    <dbReference type="FunCoup" id="A0A1E5REY8">
        <property type="interactions" value="721"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REY8"/>
    <dbReference type="InParanoid" id="A0A1E5REY8"/>
    <dbReference type="OrthoDB" id="1227494at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002182">
        <property type="term" value="P:cytoplasmic translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05833">
        <property type="entry name" value="Ribosomal_P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044076">
        <property type="entry name" value="Ribosomal_P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141:SF103">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="62"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="70"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="85"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00063629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="108" mass="10898" checksum="1DB432EA60B88222" modified="2017-01-18" version="1">MKYLAAYLLLNAAGTTPSADSIKSVLESVGIEVEDEKVSTLLSSLEGKSVEELIAEGNEKLASVPTSAPAGAAGSSGAAAGGADAAEEEAQEEEAEESDEDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REZ2</accession>
    <name>A0A1E5REZ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GTP cyclohydrolase II</fullName>
            <ecNumber evidence="3">3.5.4.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2139</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + formate + 2 phosphate + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:23704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58614"/>
            <dbReference type="EC" id="3.5.4.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; riboflavin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GTP cyclohydrolase II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.25" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REZ2">
        <property type="interactions" value="126"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REZ2"/>
    <dbReference type="InParanoid" id="A0A1E5REZ2"/>
    <dbReference type="OrthoDB" id="5569761at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003935">
        <property type="term" value="F:GTP cyclohydrolase II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00641">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10990">
        <property type="entry name" value="GTP cyclohydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032677">
        <property type="entry name" value="GTP_cyclohydro_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000926">
        <property type="entry name" value="RibA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036144">
        <property type="entry name" value="RibA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00505">
        <property type="entry name" value="ribA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327">
        <property type="entry name" value="GTP CYCLOHYDROLASE II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327:SF29">
        <property type="entry name" value="GTP CYCLOHYDROLASE-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00925">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142695">
        <property type="entry name" value="RibA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0686">Riboflavin biosynthesis</keyword>
    <feature type="domain" description="GTP cyclohydrolase II" evidence="9">
        <location>
            <begin position="110"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="354" mass="39948" checksum="C8A46C8616907A94" modified="2017-01-18" version="1">MTDTQNSNITTHTKTLPVVKCIARARIPTVQGPDIFLHLYENNVDQKEHLAIVFGEDIRSRSLYQQRGPHESQYDRMIRGAYCGKLYPGRKIADTYYSPQDTTQVLMQLHFDSVTGELIPETQTTWDSENKTMCRIHSECYTGETAWSARCDCGEQFDRAGEFIAYDGFLPILNEKNEKVKVEGGHGHGVIVYLRQEGRGIGLGEKLKAYNLQDLGHDTMEANLELNHPVDARDFSIGNSILLDLGLSNIRLMTNNPQKLKDVEYPPYLHCVERIPMVPLSWEXDGSETHGVNDSSTTLNTLTTSEMNNSATLNTNSKHAGIHSKEVEGYLRTKMEKMGHLLSKPLNLHTNKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF44</accession>
    <name>A0A1E5RF44_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2406</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RF44">
        <property type="interactions" value="126"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RF44"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="93"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="403" mass="46782" checksum="591FCEEECFC5FA3E" modified="2017-01-18" version="1">MDFTSDSIQLDQSAPAQDKNASQKNTQXFTTTASISSALSPSPKRNIKRSKTNKRESSLFYTWRSSSFFHQSDSSDEIQDDFNDSSGNLTNDVDAKQLKLIALNEERSKLLQERQKLKKEIENFKTQLKTIDHENSKNSKQNAYEQLFSLRLHWALNLPNRLNTRNPHSEITQKPVASSNRSKTVTVGPFDSLDKERANTFDMKPMVNIEHRRKYLQNQYPLLRINEALDNQDDIGSFSVEIDIDGFRFSVFTKCYNGQYYFKVEGDFLSLNLASKESRTNLNDMVKLWAKEGNIQNVIHSLYSIIEFNNTKRDFYQDILQKFMRYNKKIAESPSNEWLNLEDKGSHVTIFWEIEVDENGIIQNALAAVKNFENDKVNFTEKISELGLKEGIYRYLLELFPLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFZ1</accession>
    <name>A0A1E5RFZ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">rRNA methyltransferase 2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1878</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFZ1"/>
    <dbReference type="FunCoup" id="A0A1E5RFZ1">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFZ1"/>
    <dbReference type="InParanoid" id="A0A1E5RFZ1"/>
    <dbReference type="OrthoDB" id="20105at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF18">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005461">
        <property type="entry name" value="23S_rRNA_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="8">
        <location>
            <begin position="80"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="9">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR005461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="309" mass="35546" checksum="5A1A6F2B1774CC4D" modified="2017-01-18" version="1">MMNPFIMPRSLITLRQSTSLRSKTGVSLFNVIQNIKFPSTITYKKPLILHDFVRHNSSGKWLNRQNSDQYTSQAKALKLRSRAAFKLLEIDDKFKLFSKKYPQNVLDLGFAPGAWSQVARGRSHKDSIILGVDILPCKPPRGVNAMQANILSRKTHKLIRFFFSRKVELNRHDDLHEEHGYFEHFQEESQLESQNNTALTENIVQQPIDLIMSDMLANTSGIATKDHYSSLDLCDAALIAACDLLKPGGNFICKFYTGKEDTLLEKKFKKVFHKVLRYKPQACRDESKETYMIGMRKRQNVDKVDVFSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGF1</accession>
    <name>A0A1E5RGF1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">40S ribosomal protein S14-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1520</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ85989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGF1"/>
    <dbReference type="FunCoup" id="A0A1E5RGF1">
        <property type="interactions" value="1116"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGF1"/>
    <dbReference type="InParanoid" id="A0A1E5RGF1"/>
    <dbReference type="OrthoDB" id="10250260at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.80:FF:000002">
        <property type="entry name" value="40S ribosomal protein S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="entry name" value="Ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_uS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_uS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_uS11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_uS11_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007176">
        <property type="entry name" value="PRK09607.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ85989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="142" mass="15279" checksum="DF761D4CC290EF4B" modified="2017-01-18" version="1">MNMIIQRVGPQVRDNSQVFGVARIYASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQDVAVRCKEIGITAVHVKIRGTGGTRSKTPGPGGQSALRALARSGLRIGRIEDVTPIPSDSTRKKGGRRGRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGX6</accession>
    <name>A0A1E5RGX6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aspartate aminotransferase</fullName>
            <ecNumber evidence="7">2.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1485</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 7">
            <text>L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21824"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="EC" id="2.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="7">In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGX6">
        <property type="interactions" value="847"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGX6"/>
    <dbReference type="InParanoid" id="A0A1E5RGX6"/>
    <dbReference type="OrthoDB" id="6752799at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006532">
        <property type="term" value="P:aspartate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000066">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000001">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006719">
        <property type="entry name" value="PRK09257.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF55">
        <property type="entry name" value="GLUTAMATE OXALOACETATE TRANSAMINASE 1, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="8">
        <location>
            <begin position="36"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="417" mass="45758" checksum="FC70B186AE60D689" modified="2017-01-18" version="1">MTIDIANIFDNLAVMPADPLFQVKQNYTQDTRAYKADLGIGAYRDNNGKPWILPSVVEAEKLIQKDPSYNHEYLGMSGLPALTSSAAKVIFGEDFPVEERVASIQSLSGTGALHLAAKLLSWNNPTKSKKIYLSDPTWGNHNQLFGTCGFELATYKYWDAENKAFNFQGMMGSIANAPAGSIFVLHACAHNPTGIDPSKEQWVEILQALKQGEHLVIFDSAYQGFASGSLEEDSFAIRLAINDILKDFPILVCQSFAKNVGMYGERVGCFHVVLGASNTKAKKAVSSQLATIMRSEVSMPPAYGAKIVATILTTPELKKQWFADLVTMSSRIIKMRHALKDKLEELNTPGTWDHIVNQNGMFSYTGLTKDMVARLAXEHAVYLAPSGRASVAGVNEGNVEHVAKSINEVVNFYQSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH10</accession>
    <name>A0A1E5RH10_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphopyruvate hydratase</fullName>
            <ecNumber evidence="3">4.2.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1057</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(2R)-2-phosphoglycerate = phosphoenolpyruvate + H2O</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Mg(2+) is required for catalysis and for stabilizing the dimer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the enolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH10">
        <property type="interactions" value="1232"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH10"/>
    <dbReference type="InParanoid" id="A0A1E5RH10"/>
    <dbReference type="OrthoDB" id="1739814at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.10:FF:000001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.120:FF:000002">
        <property type="entry name" value="Enolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF1">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00178">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enolase N-terminal" evidence="12">
        <location>
            <begin position="3"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="11">
        <location>
            <begin position="145"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="347"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="374"/>
            <end position="377"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ86199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="438" mass="46626" checksum="51D4206A2BEFE953" modified="2017-01-18" version="1">MSITKVYARSVYDSRGNPTVEVEXTTKDGMFRSIVPSGASTGIHEALELRDGDKSKWLGKGVLKAVANVNDTIAPALTSSGLDVADQEAVDAFLIKLDGTPNKAKLGANAILGVSMAVSKAAAAAKNVPLYEHLNQLAYGSTKHPLVLPVPFLNVLNGGSHAGGALALQEFMIAPTGAPSFSEALRIGSEVYHHLKSLTKKKYGGSAGNVGDEGGVAPDINTAEEALDLIMDAIKAAGYEGQVDIGMDCASSEFYKDGKYDLDFKNPNSDKSKWLSGPELASLYKSLIAKYPIVSIEDPFAEDDWEAWSHFYKTAGVQIVADDLTVTNPLRIATAIEKKAADALLLKVNQIGTLSESIKAANDSFAANWGVMVSHRSGETEDTFIADLVVGLRTGQIKTGAPARSERLAKLNQLLRIEEQLGDKAVFAGKNFHHGNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH13</accession>
    <name>A0A1E5RH13_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Crh-like protein</fullName>
            <ecNumber evidence="16">3.2.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">AWRI3579_g1123</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <topology evidence="3">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the glycosyl hydrolase 16 family. CRH1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.-.-" evidence="16"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH13">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH13"/>
    <dbReference type="InParanoid" id="A0A1E5RH13"/>
    <dbReference type="OrthoDB" id="4781at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02183">
        <property type="entry name" value="GH16_fungal_CRH1_transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017168">
        <property type="entry name" value="CHR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF68">
        <property type="entry name" value="GLYCOSIDASE CRH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037299">
        <property type="entry name" value="Glycosidase_CRH1_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="10 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 21" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 19" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184768" description="Crh-like protein" evidence="19">
        <location>
            <begin position="28"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="20">
        <location>
            <begin position="27"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="17">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="17">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="33"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR037299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR037299-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR037299-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OEJ86191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="475" mass="48799" checksum="8A7CF0D3B9C67445" modified="2017-01-18" version="1">MALCDTFSLSSALMLLASAGLLTQAHAAYLTSCNPLKETNCAADSGLNANXDLDFSSESETMKYFSDYSGTGQLNYSETSGLSLTLAERFDNPSLKSNFYIMYGRMELVMQAAPGQGIVSTVYLQSDDLDEIDWEWLGTDSTQVQTNFYSKGNVTTYDRGQYNTVDGDPTSGYHNYTVDWTTAEINWYIDGQVVRTLANTTSDGYPQTPMAVYLGVWAGGDPSNAEGTIEWAGGATDYTKAPFTMNVKSLRIADYSNGDSYSYSGTSGSADSIQVSGGSINGRLSEAIASWDGSXASASSTVSASASSTVSASASSTVSASASSTVSASASSTVSSTASSTVSASASSXASSTVATSDTSSSNVAVSSTVEAVESTTSASASISATSASSSSSSSTAAPSSTAVSISATSKNDQVSSTIELTSVTSSFSTSAASTASSSATNSTKTIKSSSSGNYGQRLELTSGFLFLSLLHVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH44</accession>
    <name>A0A1E5RH44_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosome biogenesis protein ERB1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Eukaryotic ribosome biogenesis protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ERB1</name>
        <name evidence="10" type="ORF">AWRI3579_g1409</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the NOP7 complex, composed of ERB1, NOP7 and YTM1. Within the NOP7 complex ERB1 appears to interact directly with NOP7 and YTM1. The NOP7 complex also associates with the 66S pre-ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat BOP1/ERB1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH44">
        <property type="interactions" value="1095"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH44"/>
    <dbReference type="InParanoid" id="A0A1E5RH44"/>
    <dbReference type="OrthoDB" id="5571054at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070545">
        <property type="term" value="C:PeBoW complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03027">
        <property type="entry name" value="BOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028598">
        <property type="entry name" value="BOP1/Erb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012953">
        <property type="entry name" value="BOP1_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17605:SF0">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17605">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BOP1 BLOCK OF PROLIFERATION 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08145">
        <property type="entry name" value="BOP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM01035">
        <property type="entry name" value="BOP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BOP1 N-terminal" evidence="9">
        <location>
            <begin position="185"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="452"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="807"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="152"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="569"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="106"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="155"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="833" mass="94287" checksum="0DC72A20C5BD4B45" modified="2017-01-18" version="1">MSKATASALQENKNSKKVAAIKHNKKRSIPSDNEASDSEDEFVSDQENLMVDGLIDAEADESNDESGDDEFEDANEFQISEEEKRSGEDQEDSDEEYNKLLAEXXXXEEEEXXXEEDDDDDSKSITDKLSNVKLHVIEEPSVVKTEFADGTPRLLRPEIDPKYDSDDTDAEQPSNTIGDIPLSAYDEMPHIGYDINGKRIMRPAKGSALDALLESIELPEGWTGLLDKNTGGSLNLSQEELELIKKIETNQNTSGDVEEYPDYNDWFSKDVEITPLTAAPEPKRRFVPSKNEAKRVMKIVRAIREGRIIPPSKMKELRAEEEKNYDLWGDSNDDFVNDHVMNLRAPKLPPPTNDESYNPPEEYLMNEEERKAWESLEPSEREKNYVPEKYGALRKVPGYTESVRERFERSLDLYLAPRARKNKLQIDPESLIPDLPSPKDLRPFPIKCSTIYKGHTGKVRSVSIDPTGLWLATGSDDGTVRIWEILTGREVYKVIIIDDVAESEDKIDVVAWNPDKSTGILAVTCGEHIYLIVPPLFGFDVENNGKLKIENGFGFATFGNVNKGDLNITKEDNDVSDSDSDAENSSKNSNKLKSDVAQWNKPTKKQQEKDISISIACKKKRAIKKLSWHRKGDYFVTVQSDAGNTSVLIHQLSKHLSQSPFKKSKGIIMDCKFHPFKPQIFVCSQRTIRIYDLSQQTLIKKLLPGARWLSKIDIHPRGDNLIASSFDKRVLWHDLDLSATPYKTLRYHDKAVRDVEFHKRLPLFGTAADDGSIHVFHDTVYDDMMKNPLIVPLKKLTGHKVVNSLGVLNIAWHPKEAWLFSSGADNTARLWTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH53</accession>
    <name>A0A1E5RH53_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phosphatidylserine decarboxylase proenzyme 2</fullName>
            <ecNumber evidence="11">4.1.1.65</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="11">Phosphatidylserine decarboxylase 2 beta chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="11">Phosphatidylserine decarboxylase 2 alpha chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="11" type="primary">PSD2</name>
        <name evidence="14" type="ORF">AWRI3579_g1118</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer). Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine + H(+) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + CO2</text>
            <dbReference type="Rhea" id="RHEA:20828"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="EC" id="4.1.1.65"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="11">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from CDP-diacylglycerol: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterodimer of a large membrane-associated beta subunit and a small pyruvoyl-containing alpha subunit. Interacts with pstB2. This interaction may be a means to structurally tether the donor membrane (ER) harboring PstB2 to acceptor membranes (Golgi/endosomes) harboring PSD2 during PtdSer transport to the site of PtdEtn synthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Golgi apparatus membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Endosome membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The C2 domains have an essential, but non-catalytic function. They may facilitate interactions with other proteins and are required for lipid transport function.</text>
    </comment>
    <comment type="PTM">
        <text evidence="11">Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The autoendoproteolytic cleavage occurs by a canonical serine protease mechanism, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl prosthetic group on the alpha chain. During this reaction, the Ser that is part of the protease active site of the proenzyme becomes the pyruvoyl prosthetic group, which constitutes an essential element of the active site of the mature decarboxylase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the phosphatidylserine decarboxylase family. PSD-B subfamily. Eukaryotic type II sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.65" evidence="11"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH53">
        <property type="interactions" value="164"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH53"/>
    <dbReference type="InParanoid" id="A0A1E5RH53"/>
    <dbReference type="OrthoDB" id="67700at2759"/>
    <dbReference type="UniPathway" id="UPA00558">
        <property type="reaction ID" value="UER00616"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004609">
        <property type="term" value="F:phosphatidylserine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016540">
        <property type="term" value="P:protein autoprocessing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00663">
        <property type="entry name" value="PS_decarb_PSD_B_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003817">
        <property type="entry name" value="PS_Dcarbxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033177">
        <property type="entry name" value="PSD-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033179">
        <property type="entry name" value="PSD_type2_pro"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00163">
        <property type="entry name" value="PS_decarb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10067">
        <property type="entry name" value="PHOSPHATIDYLSERINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10067:SF17">
        <property type="entry name" value="PHOSPHATIDYLSERINE DECARBOXYLASE PROENZYME 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02666">
        <property type="entry name" value="PS_Dcarbxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="11" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="2 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="4 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="10 11" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_5023269223" description="Phosphatidylserine decarboxylase 2 beta chain" evidence="11">
        <location>
            <begin position="1"/>
            <end position="1295"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023269222" description="Phosphatidylserine decarboxylase 2 alpha chain" evidence="11">
        <location>
            <begin position="1296"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="99"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="733"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="50"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="215"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="525"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="63"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="88"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="218"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="249"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="262"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="359"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="532"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system; for autoendoproteolytic cleavage activity" evidence="11">
        <location>
            <position position="1155"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system; for autoendoproteolytic cleavage activity" evidence="11">
        <location>
            <position position="1209"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system; for autoendoproteolytic cleavage activity" evidence="11">
        <location>
            <position position="1296"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid; for decarboxylase activity" evidence="11">
        <location>
            <position position="1296"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autocatalysis" evidence="11">
        <location>
            <begin position="1295"/>
            <end position="1296"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="11">
        <location>
            <position position="1296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ86238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1384" mass="156923" checksum="76FEEA2B6768B09E" modified="2017-01-18" version="1">MSDSSLVSASNNSLACGVTVTANTTANTTANTTANTNANTIANATANXXANXXASPTAPTGKSETSSTNGSMNSNTKHKSNKPPSRNSNRKKKKFRYTRRNISKLKNLKKRLPEKLILTVNVLNATNLGLKNSHCNPLCCVIYDNQICTSAPIKNTNNPYWNKTFKFTLPASPKNDTIRFLIFDALDKKFNYMIEQQHARNYSNHSSISYSSYHMEKTNSQGNGHPQLSSTSNSSSSFTSSSSQSQSHSHSHSHSHSHSHSHSQSQSQSQSQHQHQTGAQNFYHLKTKSVPNAFIRENARTSNIGITNHSVYNGTNGVKDVXNPIKNNTLKXNVNNNNKSHNEGKKGKVSLLRASAVASPSSNPSFFSSASLQPSSESVDSDMGHTLEYHIDDSVDSFIDDQLYSNHQLLQSIYSNRKFIYMGELNLQISSIFANASSQLNNKLIVSPKFYDLQSKLYAKTGSQIGLSFEIIGKHMKGQNSTTKFYQDWSNSVVDFINSNKFYSMNIAGLGNGMCLLNGTHGKPSKLKDGLNNEDEISDSNSEADNDTDSDEDFISINTNNYKEAADFHHLGKQLSXSTIKSEQKLLEIFDDASNFMLGFEDPFSLRHGNTQPKDLPNSVQDCLENEQEDMISNFSDQSXLVVKLSDDGYIDYISKTLDDPFEDDEDDMYEAAPADENNYKDDIDEICTIIDDLDLGKDDSQVLMTDNLSEEDENEINSQIFQKRKKNKLVKRKKRKPQWVISKKNHASGVVIMDIIGISHLPSLKNWVRYTMDPFVILTFGRRVFKTSWKRHNLNPLFNERVIFDVYDYEESFNFDFKVIDRDSLSSHDKIAHVFIPFKQLLKHQRTHIDTVNDKDVFEIPLQFDSAVLAQNITKTPILKIKFSFLEYGDLNNKLWNRIIQNTEYYDIIELSAFLEKLGDFQPNQIYSIFEYYKKAPWSTDLITKDELIEYFNKNFVNFKLFQRCPFCNYTPERTTNNIMNSKLIVENDLVTHMSVCSVPSVMKTLKASYVSPTSASRKWVSRFFAKLINGKYALGSNNANILVQDRDTGVIVDEKISAHVKLGIRVLYKKRMTPELKNFKILLKNLTIKQGLKFDNPLSIKYIESFIKFHQLDLSECLESEYKTFNEFFYRQLNPDARIPESEDPRVLISPADSRCTVFQTVSKSKELWIKGDGFTLEKLTGGLYSVDREFTIAIFRLAPQDYHRFHSPCNGVVGKPTFIEGEYYTVNPMAVRTELDVFGENTRCVIPIETEEFGQILFIPVGAMMVGSIILTRKEGETVKRGEELGYFKFGGSTILLVINKNKVLFDEDLLKNSSDCIETLVKVGMSIGHTPDITQCMRRTKTADANQMEKLKRTISTDQSTTWEYLTTNQQLEDEYNGLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHG4</accession>
    <name>A0A1E5RHG4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alpha box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1545</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the MATALPHA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHG4"/>
    <dbReference type="InParanoid" id="A0A1E5RHG4"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008301">
        <property type="term" value="F:DNA binding, bending"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045895">
        <property type="term" value="P:positive regulation of mating-type specific transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006856">
        <property type="entry name" value="MATalpha_HMGbox"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04769">
        <property type="entry name" value="MATalpha_HMGbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51325">
        <property type="entry name" value="ALPHA_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Alpha box" evidence="6">
        <location>
            <begin position="88"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="272" mass="31792" checksum="070923AA84D37FAB" modified="2017-01-18" version="1">MQARFRLPIKKLSKVDKPRKLQTSQESFHVRLNNYLFPTEIPDPPVTLVNYLLHLILQHHKNESLTDSNNDILALSHFFCKKAKSPKVVRLTANPWIAFRSYYSRSPLVSYFQQNDISTILSEYWANNASAREIWTMFSKRYKEENKAKVKAGVLVEDFRTWLNNRFGYIADYSPLDRIIQNKLELLLPHNNVIVSHELTTDQSKKADDCWLFVEDLCLEEALLENAFEATDCTESEKKDSCCKHLFRLGAPKKTIQNKAEQLNESEHREEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHI6</accession>
    <name>A0A1E5RHI6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Signal peptidase complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1234</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Enhances the enzymatic activity of SPC and facilitates the interactions between different components of the translocation site.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPCS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHI6">
        <property type="interactions" value="88"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHI6"/>
    <dbReference type="OrthoDB" id="29558at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009582">
        <property type="entry name" value="Spc2/SPCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13085">
        <property type="entry name" value="MICROSOMAL SIGNAL PEPTIDASE 25 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13085:SF0">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06703">
        <property type="entry name" value="SPC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="208" mass="23547" checksum="31E1CB46C12753B0" modified="2017-01-18" version="1">MKSSSSDKEKVPTKVNCVYSLEEVKECLDLNLKEHLIAGSSKSLKYKEVFQYQDIKILLNIILVAIAAGSFYIDKKLDFHQSINTQTLLIISYAVVSCLYWAFTKFVERDTLFIGVAAANRGSSAKQENSSHKLYVKSVIDTENIREIQPKKSPVYKLEFVFTSDKXSDKKNVVHADCDVTRLFSEDGNLQKVVFYDLIGSSISKKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHJ7</accession>
    <name>A0A1E5RHJ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">A-factor-processing enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1276</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase M16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHJ7"/>
    <dbReference type="FunCoup" id="A0A1E5RHJ7">
        <property type="interactions" value="1026"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHJ7"/>
    <dbReference type="InParanoid" id="A0A1E5RHJ7"/>
    <dbReference type="OrthoDB" id="952271at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000005">
        <property type="entry name" value="nardilysin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000004">
        <property type="entry name" value="Putative insulin-degrading enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050626">
        <property type="entry name" value="Peptidase_M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032632">
        <property type="entry name" value="Peptidase_M16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054734">
        <property type="entry name" value="PqqF-like_C_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690:SF18">
        <property type="entry name" value="INSULIN-DEGRADING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690">
        <property type="entry name" value="NARDILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16187">
        <property type="entry name" value="Peptidase_M16_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22456">
        <property type="entry name" value="PqqF-like_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="8">
        <location>
            <begin position="39"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="9">
        <location>
            <begin position="200"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 middle/third" evidence="10">
        <location>
            <begin position="387"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="Coenzyme PQQ synthesis protein F-like C-terminal lobe" evidence="11">
        <location>
            <begin position="796"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="12">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF22456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ86355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="995" mass="115760" checksum="E0915EF8DA157F02" modified="2017-01-18" version="1">MTVLKKMNYKILENKFIKPDLDNRLYRYIQLPNKLKCLVIQDITTDKAAASLDVNVGCFQDPKHLPGLAHFCEHLLFMGSAKYPDESEYRKYLNKHGGDSNAYTSKMNTNYYFQVNHDNLHGALDRFSGFFSCPLFDEDSTEKEINAVDSENKKNLQNDSRRLYQLSKSLTNRDHPNHKFSTGNLDTLWNHPRSKGTDIREELLKFHHKYYSANIMRLCVLGREDLDTLSEWCYDLFHDVKNKDLDDLHEDYGVPVYPPDCLNKFVKVKPVKDLKTLEVMFKVPDFDKNWNGPRSSHYFGHLIGYEGNGSLLSYLKELSLVHGLSAGGHCISPGNGTFTINLQLTDNGVEEYERVLEATFQYIEMLKNNLPQEWIFQELKDIAYATFKFKQPRNPSSTVSELSSDLQKDSFYPVSNILGMFLLYNYNPEELKEFLSCLTIWNSNIFLISKKVSTDKIEKWYGTEYSVKEYSTEFIENIETVSKLPNQEFTLPRKNPYIATNFHVDTLPSDVVPLIEPLLLKSDSKTKLWFKKDDRFKLPKGYVNFDFTIPITNSTVKNVVLTSLYVSLVNDYLNDSIIYDAVIANLHINLSRHSTGLQVGFFGYNEKADILIKEFFEGISMFEPNHGRFKVFKEKLTDTYENFLFNAPYNLSSTRWAFLIYENNFSVEDRLQVLKEITYDDVVLFSKALFKEFFLETLMHGNFYKEDAMKINEIPLNSFKSTELLPYKECQLQRSCVVDDQYRYIQTTKDPKNLNSCVEYTIQFHDGVGADILGDIEDENLKSYHAHELTSSLFRLLTQTIKEPCFTVLRTNEQLGYIVWSSSYAPTSPYFFLRILVQGEYSCDYVESRIQKFLHETCVQNMHNMTDEEFEHIKKGVLGTLLQKFKNLGEETRHFLNSINSGYYNFENNTRRADIIAKHITKKHVIEFYEKYVLSSQQRLIIHTEPVNKGKQGPQEFELQNCKKIDNIQTFKSQLSLGALPTPIKKFTMEHSSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHS8</accession>
    <name>A0A1E5RHS8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Aspartate aminotransferase</fullName>
            <ecNumber evidence="8">2.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1440</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21824"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="EC" id="2.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="8"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHS8">
        <property type="interactions" value="220"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHS8"/>
    <dbReference type="InParanoid" id="A0A1E5RHS8"/>
    <dbReference type="OrthoDB" id="6752799at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006533">
        <property type="term" value="P:L-aspartate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF22">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 11" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="10">
        <location>
            <begin position="52"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ86435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="461" mass="51685" checksum="C5C7D0364EE4BA6D" modified="2017-01-18" version="1">MLTLRGSPKLIFQFKWLVNNVRSISTLNNLPKEQKDPILGLASVIKADPNPNKVNLTVGIYKDDSGTTPIFSSVQQAISKIHNKSLAYLPILGSVQYTSLVKKFLFQESMHVEPQQRYDDIVTVQTFSGTGALAVCAHFLKTYTNKKILLPNITWGNHKNIYTKSGFEEIQPYRYYNKDAPQSVDVEGMLADMEKFAAKNSVSSSSSSSSPSSSXSSSSATGKGLSPRSCVLVHACCHNPTGLDPTPEQWDRIFAKIHELQLVPIVDMAYQGLTTGNLVEDVAFLGKHINKYPWEHGFYLCQSFAKNLGLYGERIGSLSMVLPPNVTKPVRENIMLGLERVVRSQYSSPGIHGQEIVTHVLANKELKQQWFADVEKMHARINGIRKLLHEKLKHIPNFANVYNEKTQHGMFYFSNLEKPQIAQLRELYSIYLTEDGRISLSGLNSKNMDYVCKAIEKVTAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHT2</accession>
    <name>A0A1E5RHT2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Sphingosine N-acyltransferase LAG1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1389</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sphingosine N-acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHT2">
        <property type="interactions" value="605"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHT2"/>
    <dbReference type="InParanoid" id="A0A1E5RHT2"/>
    <dbReference type="OrthoDB" id="3053196at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050291">
        <property type="term" value="F:sphingosine N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016439">
        <property type="entry name" value="Lag1/Lac1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560:SF11">
        <property type="entry name" value="CERAMIDE SYNTHASE LAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560">
        <property type="entry name" value="LONGEVITY ASSURANCE FACTOR 1 LAG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="276"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="324"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="380"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="12">
        <location>
            <begin position="192"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="413"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="18"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="417"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="435"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ86462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="451" mass="52568" checksum="914D014BAFCD8788" modified="2017-01-18" version="1">MTNQTKRRSNTPELRPISSDTVATAASGSAVSGMPTNVRGRARRSSSVGNINVGDTVPSLSTMASSKKSRLASRKRLASLSEHTENTDEDLLKKFRLSYREISYRHTWLTPLLILIVVFSIYLSSANFTESNWLHQFVAISYFDPETGKYGKGIKDLSFIFFYMIFFSFFXDFLMEVILKPFSIYIGVQKPSKVKRMMEQCYSVVYYGISGPCGLAIMYHTPMWLFETKPMYASYPDLQLEFYMKLFYLTQASFWTQQACVLVLQLEKPRKDFKELVFHHIVTLSLIWASYVFHFTNMGLIIYITMDVSDLFLSLSKIFNYLESAWTAPFFGSFVISWIYLRHYVNIKVLWSLFTEFKTSGPYILDFAQQQYKCWISLPFVFVLISALQLVNLYWLFLILRILYRYVFKGVQKDDRSDSEDESDLEEEDKINQEKIMTSSRTSPNTFKREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHY0</accession>
    <name>A0A1E5RHY0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Succinate dehydrogenase assembly factor 3</fullName>
            <shortName evidence="6">SDH assembly factor 3</shortName>
            <shortName evidence="6">SDHAF3</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1622</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Promotes maturation of the iron-sulfur protein subunit of the SDH catalytic dimer, protecting it from the deleterious effects of oxidants. May act together with SDHAF1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with the iron-sulfur protein subunit within the SDH catalytic dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the complex I LYR family. SDHAF3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHY0"/>
    <dbReference type="FunCoup" id="A0A1E5RHY0">
        <property type="interactions" value="265"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHY0"/>
    <dbReference type="InParanoid" id="A0A1E5RHY0"/>
    <dbReference type="OrthoDB" id="278329at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006105">
        <property type="term" value="P:succinate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20270">
        <property type="entry name" value="Complex1_LYR_SDHAF3_LYRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008381">
        <property type="entry name" value="SDHAF3/Sdh7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13137">
        <property type="entry name" value="DC11 ACN9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13137:SF6">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13233">
        <property type="entry name" value="Complex1_LYR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="110" mass="12974" checksum="E363AC67059502C1" modified="2017-01-18" version="1">MNKVKPLIPPLQLYKKILREHKYLPQIQRELGDQYVKNEFKLHKETDNPLHIVGFLTSWQDYLHTITQGKWVEGSLSKDVLGKMSNEQIGQLYELMKETQHKNIIKGNEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI68</accession>
    <name>A0A1E5RI68_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1177</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RI68"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="540"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="23"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="241"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="553"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="566" mass="64861" checksum="88FFDC884BE476D5" modified="2017-01-18" version="1">MENYAEELISQFYAEEQEYVFKIKTLQKTQETLLDENLRLSENCASWKVKVQDLHQKLDLAKNEKVEHEKKIEGMKQTALTECETFGKKMNLLKSQVSELHKERTCLTNEKNLLEASVKKFESLNISLGSLKEENALLAKEKVSYDEEKKTLQSELASRNFELTLAKANYNTLLQKNANFEKKTKENDAKSVDLQEKLSIAQSAHEHCSKKKTEYIKRIEELXTEKTSILEQNQKEIVINNKNFEQAKTILETECKELKKKLANAEELRKKDAEEIAELHEILERLQSDNKRQLAKIQETKEKNKELECDTLEKNQELADQVEKNKELANQVFELTSRTKTFEEELNNWKTKKLYTCKIFELNYRFISLLLNKPCTSLQTFAVLSDSSFRKISSSLLKNVAKNNNSKVSSMEELILTIDESQNHHEKTGKSIDVSITEEHASLITKEDTLTNLPNKQRDTLRFIGDKDSFTEKAIFTSNPFDPVVRAEPPADKFESCTVNSSASSIVSEITDPLNNKPIASTEPLEPFVIADSLNAKSVSKRASRCGGKKTLQSKGKGSRQQQKRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIE2</accession>
    <name>A0A1E5RIE2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">40S ribosomal protein S4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1163</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic ribosomal protein eS4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RIE2"/>
    <dbReference type="FunCoup" id="A0A1E5RIE2">
        <property type="interactions" value="1273"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RIE2"/>
    <dbReference type="InParanoid" id="A0A1E5RIE2"/>
    <dbReference type="OrthoDB" id="1109245at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06087">
        <property type="entry name" value="KOW_RPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000005">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.740:FF:000001">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00485">
        <property type="entry name" value="Ribosomal_eS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000876">
        <property type="entry name" value="Ribosomal_eS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032277">
        <property type="entry name" value="Ribosomal_eS4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013845">
        <property type="entry name" value="Ribosomal_eS4_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038237">
        <property type="entry name" value="Ribosomal_eS4_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041982">
        <property type="entry name" value="Ribosomal_eS4_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013843">
        <property type="entry name" value="Ribosomal_eS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018199">
        <property type="entry name" value="Ribosomal_eS4_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16121">
        <property type="entry name" value="40S_S4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00900">
        <property type="entry name" value="Ribosomal_S4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08071">
        <property type="entry name" value="RS4NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002116">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00528">
        <property type="entry name" value="RIBOSOMAL_S4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="RNA-binding S4" evidence="8">
        <location>
            <begin position="42"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="261" mass="29221" checksum="82A2A53572B52335" modified="2017-01-18" version="1">MGRGPKKHQKRLSAPNHWMLDKLSGAYAPKASPGPHKLKESLPLIVFLRNKTKYGLNGREVKSILMQRHVKVDGKVRTDSTYPAGFMDVITLEATNENFRLIYDVKGRFAVHRITDEEAAYKLGKVKKVQLGKKGIPYVVTHDGRTIRYPDPSIKVNDTVVLDLASGKITDFVKFDTGKLVYVTGGRNMGRIGTIVHREKHEGGFDLVHIKDSLDNTFVTRLGNVFAIGEPGKPLISLPKGKGIKLSISEERDRRRANYGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RMZ6</accession>
    <name>A0A1E5RMZ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Protein SWT21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g635</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RMZ6"/>
    <dbReference type="FunCoup" id="A0A1E5RMZ6">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RMZ6"/>
    <dbReference type="OrthoDB" id="239865at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ88256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="393" mass="45153" checksum="4D3044E60E328105" modified="2017-01-18" version="1">MQKNRSPTPTPQYISTCIDAQWFSDGRGVVTIHSDHTIQVCISPTNNFENFYTRRWVKNQSMMAGMVVPGTTSILLSCKNMPLQMYNLPLDSALNGNLPQSYETPSTYLPSATFTYNTTFRVHNEAFSPLFSIISHPFEQSRFYTGTNKARIDTFDMQHKDPVRTWEPRPKRRRGVGHVSALSTCKADPSVLYAGTSQHAVFHVDQRTKDMDYMVSCSNGVAQILQSDNANYLYLIPKRFCDDNQTIKVWDLRKDDFVAKLHLPMTKSSNKELKMKNSKCSMNSLHGLLVGSPFDSSYIYKWDKIVVETGGVNAYSEEQNFPTDIIHYQDKFDLQDENLYTRIIETNPKNNHMAFICCTEKYRTGYKSVLKDKEIIGGKFTRSQPQRSIFFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN08</accession>
    <name>A0A1E5RN08_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g625</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN08"/>
    <dbReference type="FunCoup" id="A0A1E5RN08">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RN08"/>
    <dbReference type="OrthoDB" id="5328412at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="470"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="565" mass="64968" checksum="8F7C087D864566D2" modified="2017-01-18" version="1">MAKKFNFPKKSKGSSTRKVDKILQEQTDFYEEGVELEELAERWVLSDIKKTLNFYYQAVTTYNAGLQCTVNRSDKISYNIAYNRLTLLLKLQSEYKHVNGDTDILVYVVGLAPDLLQMLFPENMVFHLLGQFDELMVLSESNSLATWDLYYNYFTAILLFLSTRDSYMADITGAEIVQLLDKFSYIFKKLVDHHVEFFDSVQPNIMSDEEKTRQEFLDYPNLEKDELLKSSSFDVNTGEGIKLANNSASNDQNDNTDMQSQNMSEVVESSSSMSWESYFDTLNVANTFVNEILELIISHDSTEGSAQLSTSDGNDFQKNYILVNMNKLHLQIIGLFDTAKDSLQSSSEPISAKDPTEELTLSLRYTDYLTHYSEYSYLWNKSNEVDPQTCNSSDYLLNEIDFLRLVCNLENVATLFTPDERWNMATKLDKTLVRAIQLISKEKSSIATGLIKGRDNELSPLTTTLSRLYIRRSENELNRLDLQKQKYGNLHTSLQDLQEEQQQQKQIEAVEKTIRVLQKNVVMLCNNAMNVAKQSCGVREYINDKLVRNSVYQEATELQATINFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN18</accession>
    <name>A0A1E5RN18_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cytoplasmic export protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN18"/>
    <dbReference type="FunCoup" id="A0A1E5RN18">
        <property type="interactions" value="1134"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN18"/>
    <dbReference type="InParanoid" id="A0A1E5RN18"/>
    <dbReference type="OrthoDB" id="447103at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006409">
        <property type="term" value="P:tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051177">
        <property type="entry name" value="CIK-Related_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984:SF3">
        <property type="entry name" value="N-TERMINAL KINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984">
        <property type="entry name" value="SCY1-RELATED S/T PROTEIN KINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="574"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="684"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="739"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="750" mass="84915" checksum="6F31F5DD77132BEE" modified="2017-01-18" version="1">MNFLRNTFSSFPYYKDPDPVFSTEFWTVYNGTSKKDSKVTCCIFEPNPVYLQQNKGFYTNALTNAKTLRFPGLVKVLEALEDYGHCIVTERVTPISTFDYTKHDNAVALGLFQLSDLFQSLANNKIEMPPVKLENIYINNVGEWCLFQFPDSSAHQENRTAVQQFVQFIKQENYNTQLGIPNNTLLKLANLQSHLLSHYTIVQVYQILKKEYPFLTHFNDSIDCLMELSKLLLPLNRVQKNADDLLKGIPIEFTSNFLFPPFFHYLTMGNGTGAQGQTVSPEANLLFLMTVLYNCTHLDIKLFENVILSQFDKRDRQITFLLLKFQKQYIPTTTDVPSNYAAFYKNLYPRLVACLKDQDSQIRFETLKVFSNGFIDTMDLRSINNDFLRHLAEVQQNDKDPLVKNLVLKILCTQISEKLIGYHGKSKGLIILSAALCKHLNPKESVAVKKNVLHLLAEKIEQTDWPMDVLVSKLLPGVAPCMMDKNTSVRKHANQVFNMICNEIKRQASLKESADETQSSEGDDEDFDEAALNKYDMQIDSVTLVSEMFKSLGLSSNADFSSFHTDQSIPFNANAAVKPPKINSPDNSLYEKPNKLSTPDASLWEESQDAGWDSWNDDTQATEPQPKSKAFTEKTIAKTKPSWLGDIDDNGKGDEDTWFPESKKSATKHGVVKKSSWLDEEEVASNNIGGTSSSTLNNKTGGGRTFRLKTNAATKKPAFANKKPIAKNKTAFKDLDIRGDDDDDWGNDDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN99</accession>
    <name>A0A1E5RN99_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 20">DNA topoisomerase 2</fullName>
            <ecNumber evidence="7 20">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">AWRI3579_g677</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18 20">Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19 20">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6 20">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 20">Belongs to the type II topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="7 20"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RN99">
        <property type="interactions" value="1211"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN99"/>
    <dbReference type="InParanoid" id="A0A1E5RN99"/>
    <dbReference type="OrthoDB" id="276498at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000819">
        <property type="term" value="P:sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16930">
        <property type="entry name" value="HATPase_TopII-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00187">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03481">
        <property type="entry name" value="TopoIIA_Trans_ScTopoIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03365">
        <property type="entry name" value="TOPRIM_TopoIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.40:FF:000011">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.30:FF:000001">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000008">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000004">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.670:FF:000001">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.199.10:FF:000002">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="entry name" value="Topoisomerase II, domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.10">
        <property type="entry name" value="Topoisomerase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050634">
        <property type="entry name" value="DNA_Topoisomerase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001241">
        <property type="entry name" value="Topo_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013758">
        <property type="entry name" value="Topo_IIA_A/C_ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013757">
        <property type="entry name" value="Topo_IIA_A_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013759">
        <property type="entry name" value="Topo_IIA_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013506">
        <property type="entry name" value="Topo_IIA_bsu_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001154">
        <property type="entry name" value="TopoII_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018522">
        <property type="entry name" value="TopoIIA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031660">
        <property type="entry name" value="TOPRIM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034157">
        <property type="entry name" value="TOPRIM_TopoII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169:SF38">
        <property type="entry name" value="DNA TOPOISOMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169">
        <property type="entry name" value="DNA TOPOISOMERASE/GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00204">
        <property type="entry name" value="DNA_gyraseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00521">
        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16898">
        <property type="entry name" value="TOPRIM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01158">
        <property type="entry name" value="TOPISMRASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00418">
        <property type="entry name" value="TPI2FAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00433">
        <property type="entry name" value="TOP2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00434">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="Type II DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52040">
        <property type="entry name" value="TOPO_IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00177">
        <property type="entry name" value="TOPOISOMERASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15 19 20" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="16 19 20" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14 19 20" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="22">
        <location>
            <begin position="440"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IIA-type catalytic" evidence="23">
        <location>
            <begin position="689"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1069"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1192"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1341"/>
            <end position="1472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="1069"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="21">
        <location>
            <begin position="1193"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="1270"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="1296"/>
            <end position="1305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="1344"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="1354"/>
            <end position="1364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="1380"/>
            <end position="1390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="1444"/>
            <end position="1472"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="19">
        <location>
            <position position="779"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00019635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00053943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU362094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS52040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="OEJ88358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1472" mass="167821" checksum="24DEAAAFE7243E16" modified="2017-01-18" version="1">MSSPTKSSSDKYQKISQLEHILKRPDTYVGSVEMQEEKQWIFDEDTECMIEKEVKFVPGLFKIFDEILVNAADNKVRDASMKKIEVTIDAEKNEISVKNDGSGIPIEIHNKEKIYIPEMIFGHLLTSSNYNDDEKKVVGGRNGYGAKLCNIFSTEFVLETADPSKKKKYIQKWENNMQTCNPPTIVSYNLKSSYTKITFKPDLKRFHMDVLDKDILGIMNRRVYDINGSVRDINVSLNGKSIKIRNFKSYVELYLKSLEKWKRIQKGDTNMDNLETPTIIYERFNDRWEVAFAVSDVSFQQVSFVNSIATTTGGTHINYICDQIIKKIQGKLKKKNVKPFQIKNNIFLFINCLIENPAFTSQTKEQMTTRVKDFGSKCEISDEYITKIMKTNLSDRIFEIADENADKALKKTDGVKKHRITEYPKLEDANKAGTREGWKCTLILTEGDSALTRAVAGLAVVGRDYYGCYPLRGKMLNVRDASSDQINKNQEIQAIKKIMGLQHKKRYEDVKTLRYGHLMIMADQDYDGSHIKGLLINFLETSFPGLLEIPGFLIEFITPIIKVSITKPRKQTLQFFNIPEYSKWRDEESSRYTWSYKYYKGLGTSGEQEMREYFSNLDLHLKTFHSLQDDEDEVIELAFSKKKADARKEWLRQYEPGTHLDHSLSEIPIKEFINKELILFSLADNIRSIPNVMDGLKPVQRKVIYGSFKHNVFKDTKVSTLAQYISAATEYHHGDAALQQTVVGLAQDFVGTNNIYLLIPKGAFGSRSTGGKDAAASRYIYTHLNKLSSEIFNTKDQPLLKYMQEDDKTVEPEWYAPVLPMLLVNGSEGIGTGWSTNIPSFNPLDIIQNIYRMMDGKEMEGMTPWFRGWTGTLEKIDAVRYKTFGRIEQIGPTTIEITELPPKVWTINIKEHLLQGIGGTEKVKPWIKDMEEQHGSTIKFVITLTPEEFEKTRKIGFYERFKLVSSINLSNMVAFDHNNKIKKYDDPRDILEEFFYVRLEFYLKRKDYMIANIKKVIEKLEYQIKFIQMIISGELKISNKPKKQLSAELHERGFPRINKKGDIIMVKEEEEDDDEDDEDEDNNTTNVATGGLDMPDAESQNISASNVIKNGFAEKYGTYDYLLSMKLWSLTMEKYNKLMANIQDLKKELDTLLNKTPTDLWKEDLVNFEKAYHTFIAYDNDVRNSIIPNKATGTKKKKKVTATSKKVKKETSPTFDRILLQPAKTPMKSATKIKKESSSDDLSGSKGQKEPGKKNLNSKIKKELSPSAPPTSTTTPISSNSTTPIDTTKAVKEEPNSINLSNAGSKSADILSSDSDADSELDVLKNFNSKFNKVSSKFDMAISSDEEEEEEEKTSENKVNKRSSESVSDDDTPAAKTADENDDGDDDEEKDFALALKKRKLNNSNGATKTYSKRPTRSTRPQRYTFSDEDSGASEQSENGLSGGDDDDDEEEGDGGDDDYDQQDGDESFVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNC5</accession>
    <name>A0A1E5RNC5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Ras-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g693</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The S.cerevisiae Ras proteins modulate the activity of the adenylate cyclase catalytic subunit and therefore affect the biosynthesis of cyclic-AMP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNC5"/>
    <dbReference type="FunCoup" id="A0A1E5RNC5">
        <property type="interactions" value="551"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNC5"/>
    <dbReference type="InParanoid" id="A0A1E5RNC5"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097271">
        <property type="term" value="P:protein localization to bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000080">
        <property type="entry name" value="Ras-like GTPase Ras1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="252"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="252"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="333"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="380"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="405"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ88385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="441" mass="49054" checksum="9FADDC6760B8BBC6" modified="2017-01-18" version="1">MNNNIREFKLVVVGGGGVGKSALTIQLTQSHFVDEYDPTIEDSYRKQVVIDNKVSVLDILDTAGQEEYSAMREQYMRTGEGFLLVYSVTSRNSYEELITYYQQILRVKDSDYVPIFVVGNKCDLEEERRVSYEDGLQLGKQLHAPFLETSAKQAINVEEAFYGLARLVRDNGGFYNPMNETTDQINGTGLQNTYNTNQNTYTNGVNYQKIGNGQSSFDMNFDENDNSITTDNINTNIDNNIDTNIDTNIDTNTNTNTDTNNNNHNNNTNNNNYYYSNPTTKSNTNNTSSNNNKITSTDSTSPLQTNQTTTTNTAATVKSENNTRANGYDKDNNNIMNNNIQSQQNAAVQKQQQQMHQQAGLQGVKKERVFSNANQDLDAPSTNSTTSPNTSFRGDNVQGLANQSKTTQQKQTQQKKTNTGGNSSTKKPEGKKSDGGCCVIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNE9</accession>
    <name>A0A1E5RNE9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Bud neck protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g665</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNE9"/>
    <dbReference type="FunCoup" id="A0A1E5RNE9">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNE9"/>
    <dbReference type="InParanoid" id="A0A1E5RNE9"/>
    <dbReference type="OrthoDB" id="3973268at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="51"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="166"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="265"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="310"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="56"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="77"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="209"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="276"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="398" mass="43796" checksum="993804AEF7E86F6A" modified="2017-01-18" version="1">MEVDMPEDSNGEKSLKSTRRNSENFKKRLSQIELEINEMNDLLTENIDMTQKMMIDQEDSGATEDAGDMSSGPTITNEEKEKEPVAIDGKKEEKETKAYNDAIEHSVEAERPPTKQADGSSWSDVSTTLPAASSPSNGDLEDSALMKEILDTPFNFSHLKSADAVTSPHSATSSSYSHSSDASKNGVKSKIQSIEAINSECSSPKSSSNHQESGESVPTISNYKRDNLNAFTTDNASNSLSAPRILSNSVKSPFRVISVSKVSGHSSTSFEKSNDQDTNTAAQPATQREAFIGQSGSQKAESTKHKKMTVSSLQLVYEHLDNKIQKVEKEIDYLQNIMVTGGLNLADSKKLKEGIQILENYLDSKVKLRYEIGVKLSRKLRHRIELGKNEEVFFSGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNN3</accession>
    <name>A0A1E5RNN3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Asparagine-rich zinc finger protein AZF1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g714</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNN3"/>
    <dbReference type="InParanoid" id="A0A1E5RNN3"/>
    <dbReference type="OrthoDB" id="3972941at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000130">
        <property type="entry name" value="Spalt-like transcription factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002157">
        <property type="entry name" value="Transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050717">
        <property type="entry name" value="C2H2-ZF_Transcription_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196">
        <property type="entry name" value="ODD-SKIPPED - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196:SF12">
        <property type="entry name" value="ZINC FINGER PROTEIN 208-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="1128"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="1156"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="1184"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="1212"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="110"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="172"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="286"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="426"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="719"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1027"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1089"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1277"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="9"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="198"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="213"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="232"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="246"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="291"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="346"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="368"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="426"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="727"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="743"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="796"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="807"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="846"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1106"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1284"/>
            <end position="1311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ88283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1349" mass="144485" checksum="157E439511A42325" modified="2017-01-18" version="1">MSKKDANFATDSQVPFENASGKQNATKNSGQNKNLDVENIQGGDSNSAIMSGNEDHDTFDVSKHFSDDRRDSTDVFLKPRQDSMSLYTNFMPRLSNATESSIANYLNMDSNNNNRGTTPDVHRGYSIVNNNMFAPGPSGSARSSSSRRQSVVQGTSGAVGSALNSNIAQQNTFNGARRPSEQLEPFVPPMKFKTPNFGSSSTGTGNYTTNNTALKRSNSGTRGSKTKHKSATPVSSFGTQDSNRRGAAANILSNNNNNNSSSDNLGSTGLTADSSVNQNAALTGEDIISSNDNNNNNNNNNNNNNNNNNNNNENNNNKNNSNNNNNNNNNNGVDDEKQSNQISKTNVPATPNNNNQSNKNHENRATTKNESMTHSSNTSPNLHSQLPELPALRSDSISSILGAAAMINQPLQPPSKRNSIFMPTDQLTSNTTQNNEHGTMKPPSTTAPTYYNKQQQHQQQQFQQLLNSNSLGNLYSDANNGGNHITDNIDFDIFSNLNTSRKSSFRFGDDFDLSAFRRRDSSVRGTLDLPPLPGSSISNRLRAGSRTFSQNFNHHPFSATSGPNNTNATPNTITGNGKKEEQEHFKNSRYSNFLKNNENAISDYEDDDLIDLHGLTRATSIGAGNGAHVHSNSNQLHNNNQGLMDSRMIMRRKSNLRDNSMTPIPLTTPPLTGFGHAALPNINNSSAVSDPLLGLDSSSHMDVPPSLTFDANGNIVPEEIAPAQKSSSKKSSSKKSSSKKSSTKTSSSKSSSSKSSSTKKDSSQKSRKRKSSSNTETGTGADGKESKPKKRKTSKKTSSSSSTSSGHKNDHPETSSKSTDEKSGSKKSSRSKKDSKSKSSKKSKKGSSTTSSSTASDKSDATKPSTKKKRSSTSSSSKPTKKVKSRGSALSNKVPESLISHPIIVENPMKNTNGTTDKNDNPVLGATRVDQLMLILQARQKGFTTALETTKDGKINIDNAPEIIPPAIELVGGVDKPKTHDLLLNPMSHSPSITTEALLNSVGGVKPDPDGVLMTPSSTLAVPAFATQKSPSQAAKSPNQTEISLSKMASPQNGKGTSAIGTPLMQSAATIASPLEKSELDTAQAVPAVSTPSTNSAIGVAGTPNGAGSPVSATGSASGSVSGKRKLHVCQYCHKHFTQSTHLEVHVRSHIGLKPYECSYCGKTFTQGGNLRTHIRLHTGEQPFQCNVCGKKFSRKGNLQAHSLTHENFRPFQCILDGCDKSFTQLGNLKSHQNKFHLDTLNVLTMKLALNNFDLENEEERRLLEYLSSLYKNSNKGIKGRGKNSASAQKSNPGTPSQKPGSPSLTDSLGTTAVPVSALPVAPTEVQKQKNEPMLDEAAVAALTNSSDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNN6</accession>
    <name>A0A1E5RNN6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">mRNA stability protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g766</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Plays an essential role in initiation of the G0 program by preventing the degradation of specific nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the endosulfine family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNN6"/>
    <dbReference type="STRING" id="56408.A0A1E5RNN6"/>
    <dbReference type="InParanoid" id="A0A1E5RNN6"/>
    <dbReference type="OrthoDB" id="5949865at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006760">
        <property type="entry name" value="Endosulphine"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10358">
        <property type="entry name" value="ENDOSULFINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10358:SF6">
        <property type="entry name" value="ENDOSULFINE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04667">
        <property type="entry name" value="Endosulfine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="119"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="206"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="120"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="155"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="173"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="227"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="269"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="301"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="344"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="360"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ88501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="386" mass="41270" checksum="EBA787D989A1F71A" modified="2017-01-18" version="1">MSHNETKIPERELSPKSVVDPKNPNNTMKADIYNGTSSIDENVLKTLNPQEAKLYKMYGKLPNKKDLIHRKLQERKYFDSGDYALSKANGGDGVKYQHFNNLPLTNPSGLRESIIKRRMSNGSPNEDGGSVSNNEGHGRPSIPLSPPSGDSPVLVSQNSTSSVKSSVSSNLNPHPSSISGSNVLGGNSITKAPAIPVVPAAPVVPTIPSGSSRSSSLRSSVSGSSLKMQHSKSNSITSNRSLSGYSTDTPRSSPGNSPLALSQLRSPPLNSNDSLNSLSSLNSLDSNTNDSNDSNDFDNSTYIEKSNPNSSHQSIDTGSDNVHNIKKTNSTSANTPLSQSISVSHDKDSHTTTTTERDYNSPTGLMSESSKTKTTTDYFAPKDHHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNR4</accession>
    <name>A0A1E5RNR4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Glucose-6-phosphate 1-dehydrogenase</fullName>
            <ecNumber evidence="3 9">1.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g576</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15841"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="1.1.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glucose-6-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="3 9"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNR4"/>
    <dbReference type="FunCoup" id="A0A1E5RNR4">
        <property type="interactions" value="506"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNR4"/>
    <dbReference type="InParanoid" id="A0A1E5RNR4"/>
    <dbReference type="OrthoDB" id="60984at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00408"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000015">
        <property type="entry name" value="Glucose-6-phosphate 1-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00966">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019796">
        <property type="entry name" value="G6P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022675">
        <property type="entry name" value="G6P_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00871">
        <property type="entry name" value="zwf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429:SF0">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02781">
        <property type="entry name" value="G6PD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000110">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00069">
        <property type="entry name" value="G6P_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 9" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="6 9" id="KW-0521">NADP</keyword>
    <keyword evidence="7 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase NAD-binding" evidence="11">
        <location>
            <begin position="23"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase C-terminal" evidence="12">
        <location>
            <begin position="225"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="81"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ88535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="528" mass="60712" checksum="775B1CBF94054FBC" modified="2017-01-18" version="1">MSSTPENFNHKPVNFEENTIITVFGASGDLSKKKTFPALFGLFREGYLHPSVKIVGFARSDLSTQDLRERVKPNLKKGKHGNHEETNQNNEAKNKIEQDFNKKVDDFLNLIEYCSGPYDKDEGYEKLAKIYADFEKTRNGGKECNRLHYLALPPSVFGTVGERIKKNIYIKNEHAQTRVIVEKPFGHDLESSRALQKELAPLFDEDEIYRIDHYLGKEMVKNLSVLRFGNSLLNAGWNNQHISNIQLTFKEPFGTEGRGGYFDQIGIIRDVMQNHLLQVLTLLTMERPVSFDPESIRDEKVKVLKAFAPINKKDIITGQYGKSEDGSKPSYLEDETVPKDSKCVTFAALAFNIRNERWDGVPMIMKAGKALNEGKVEIRIQYKKVASGVFNDIPNNELVIRIQPDEAIYLKCNMKTPGLSTSTQMTDLDLTYARRYQDFWIPEAYESLIRDALMGDHSNFVRDDELDYSWKLFTPLLKYLESDEAPTPEIYPYGSRGPQAMDQYLMDHKYEFNSSEKYQWPVTRPENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNS2</accession>
    <name>A0A1E5RNS2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Quinidine resistance protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g808</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. CAR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNS2"/>
    <dbReference type="FunCoup" id="A0A1E5RNS2">
        <property type="interactions" value="70"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNS2"/>
    <dbReference type="InParanoid" id="A0A1E5RNS2"/>
    <dbReference type="OrthoDB" id="440553at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF51">
        <property type="entry name" value="QUINIDINE RESISTANCE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="364"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="424"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="449"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="490"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="514"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="83"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ88531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="566" mass="62766" checksum="9D3FF55ED7701433" modified="2017-01-18" version="1">MLNQIEQKETLPYMKDNSSSLHSKSNGELITLREQHSGTSVSSNCDEKDSLQQDAYGDLDDAFADEDAKYPYCVFPQKIVVLIVVICATEGLLSTIAASSFFGCLDEIRSHFHVTNETVNLAIVVYFICQGVAPTCVTFMIDSGYIGKRNMVLVGCLIYATTCVGIANAKNFGTILGLRCVQSFSISPVIAINSSIASDITKKEKRGNIVGLVGGFQVCAGSAFGSVISAGMTNQFGWTGIFYYLAIMGYAAFVICFLCLPETKRTECGNGSLLPLKWYDRAPIVKTSYWKKNFLSKPYEQSRQETKKTNFFGFFEIIKKTEMIHLLMASGMQFCLWTCHLTSLSTVFSNSHYNMSTLEIGKCYLPSGVCTLFSIVGGGRVLNWNYKRRKTQYDAWIQTQIKNEDFDISLPKYKFNIYKARLEFANIPLLISGVSFVICGWLMQTKQSVAAILVFSGIGCLFSNCILTFSTTLTVDLHPFAGSTAAASVNLSRCFTSALFVGVLDYMFDAMGVGGTFVLIFGITAAFSIGLIYTIKYGTEWEYNRLNGIKSTRNFWQTCKKYITIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNS7</accession>
    <name>A0A1E5RNS7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chitin synthase export chaperone</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g670</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Chaperone required for the export of the chitin synthase CHS3 from the endoplasmic reticulum.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with CHS3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CHS7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNS7"/>
    <dbReference type="FunCoup" id="A0A1E5RNS7">
        <property type="interactions" value="63"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNS7"/>
    <dbReference type="InParanoid" id="A0A1E5RNS7"/>
    <dbReference type="OrthoDB" id="2189463at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022057">
        <property type="entry name" value="Chs7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35329">
        <property type="entry name" value="CHITIN SYNTHASE EXPORT CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35329:SF2">
        <property type="entry name" value="CHITIN SYNTHASE EXPORT CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12271">
        <property type="entry name" value="Chs7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="96"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="125"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="161"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ88363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="355" mass="39334" checksum="4128AF84DE277B7B" modified="2017-01-18" version="1">MSQNGFVNNICQNTPIPMCFLIRAANDLSFASSSQSPDVFQGFGAISLCYSRSVNLANTTIFQIPTAFIYFITIATIMIILNGINKKYTAIGRSEYSIFFQLVLISVFLQMVVECGVVPHHSFVFVHFVSLQIAMTGCVCALLFTNGLLGFKIWEDGTRKSYTILAVIGVVAFVANFLVAFGSFNDWFGVSNHNTVIQFATTAEGNINKDVLLNTIEGITNTMNSLIPLNSNRNIKQTKVIGILVVNYGLNAIFILLYVVSQIAISILIVKNYWISGTVILGAFFFASGQLFLYKMSLLVCTASNHYIDGLLLQSLGNLFAFMMIYKSWEISTQEDLEFSVTSHKTMPNGHEKIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP14</accession>
    <name>A0A1E5RP14_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pro-apoptotic serine protease NMA111</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Pro-apoptotic serine protease nma111</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g725</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP14"/>
    <dbReference type="FunCoup" id="A0A1E5RP14">
        <property type="interactions" value="136"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP14"/>
    <dbReference type="InParanoid" id="A0A1E5RP14"/>
    <dbReference type="OrthoDB" id="4217619at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06786">
        <property type="entry name" value="cpPDZ1_ScNma111-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06719">
        <property type="entry name" value="PDZ2-4_Nma111p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025926">
        <property type="entry name" value="PDZ-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46366">
        <property type="entry name" value="PRO-APOPTOTIC SERINE PROTEASE NMA111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46366:SF8">
        <property type="entry name" value="PRO-APOPTOTIC SERINE PROTEASE NMA111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12812">
        <property type="entry name" value="PDZ_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="PDZ" evidence="9">
        <location>
            <begin position="355"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ-like" evidence="8">
        <location>
            <begin position="421"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ-like" evidence="8">
        <location>
            <begin position="903"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="43"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ88631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1027" mass="113657" checksum="E33E3F341515AFFA" modified="2017-01-18" version="1">MVFCCLCSKFPSNNNKIQKKNSNGNAPQASLIFMSKRKYQFSLDGKNNSSPKPKHLRVSNGQKAEQQHEHQSVYSDSDPEDTNDLELMSTNNINAKDNSGWHKTISKVVQSVVSINFIQLAFDADESVCSEATGFVVDAKRGYILTNRHVVSPGPLVNAYAIFNNHEECELTPIYRDPVHDYGVFKFDPKKLKYMELQQLELKPSLANVGLEIRVVGNDAGEKLSILSGFISRLDRNAPDYGEMTYNDFNTEYIQAAASASGGSSGSPVVNKDGYAVALQAGGSSQASTDFFLPLDRVHRTLTCLQQNKPITRGTIQTQWLLKPYDECRRLGLSADKESLMRENFPDKCGLLVAETVLKEGPASELIAAGDILISINEELITSFIQVDRILDESVGKTIELLVQRSGKNVFAKCTVGDLHAITPSRYVEVCGASFHELSYQMARLHGIPVSGVFLNSSTGSFDFNDDEDAGWIIDQVDNNPTPNLDVFIDVMSKIPDKKRVICKYHNLIDPHTFHFFPVYVDRHWFKKFKMYTRNDETGVWDITNLADPIPSLPSAKKTAKLFETSFNETGLNELAKSIVMVSSKIYVHIDSFTPDFDITKVAGLVVDAELGLVIVSRIVVPHNCLEVFITIANSIIVPAKVLFLHPTKNYAIVQYNAQEIDCDLQSANLAQTPLVRNCDTHFLGCTVNDTLLSAPASVANIAPFSVGSNVVPRYRGTSIEGISINCPVSQTCSSGILCDKDGIVRALWLLFLGPKGNGSSKLFFRGLDVVDFKEVVTEIQKGIKSANTYSVPSVYTINADVKGITLLKARISGVSDEWIGRMEKESSSVAQFMAVSRVSYTEESDSLKKLQAGDIILSKNGALVKNTRDIMNELSNDVLELKVVRNGEPVDLQVKPFEVKETDSLVIFSGCLVQEPHNSVLQCVSSLPSKVYISDCLSASPASQYGVASTNFITHVNEQETPDLDTFIKVIKEIPDKTYCKMNLVTFESVPFAISLKTNYHYFPTSELHMDHTTNEWSVKKVREGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP21</accession>
    <name>A0A1E5RP21_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mitochondrial import receptor subunit TOM70</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g724</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Tom70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP21"/>
    <dbReference type="FunCoup" id="A0A1E5RP21">
        <property type="interactions" value="798"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP21"/>
    <dbReference type="InParanoid" id="A0A1E5RP21"/>
    <dbReference type="OrthoDB" id="2942533at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46208">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46208:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="160"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="406"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="440"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="508"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="585"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="46"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="50"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="77"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ88630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="658" mass="73028" checksum="A9055FBECB14FBB4" modified="2017-01-18" version="1">MSEDHSLGTFITRNRTAIITTVAAATTVIGAGYYYYNNVAGKSFDESDADSSSSLKSSSSSPSSSTPSTAGSASSANKKKKKKQQYKQKKKLEKQAENAAISGTLDEQAVGVAGTESSKDKQSGTKSEIVDLSNLGVPLTSTGDIDMTQLTTEEEKDKITMKLKEQGNVYFKNKEYENAIKLYTTALNIKKDPVFLSNRSACYVSLGEYEKVVEDCSAALELKRDYSKCLLRRASAYEILGNYSESMFDISALSLLGDFNGESIETMLERVLNKQITKVLEEKVKDATTKTEELSSFSDLASFFSSLSSKHDLKHYNPEDEDDVLLAKTIDDLYSQSAKGLSNANENFPKCVSRFEKKLAESPKDEILKEKLAIALSYQGILYFFKNDPTSANAVFEKSLELFPTVNTYVFFALLKSEKGSSDEVFGSFAKAIELDPKDPAPYYHRAQLYFVTNDLKKAGADFEKAKEVDPNHVFSYIQIACLTYKEGSLTDAETLFSQALRKFPDNSDIYVSYAEVLADSKKYEKSIENLDKAIELEEKAGNENVSANALITKANILASNPTVENFVEASQLLEKANEKSPRNFKAKISLGQFKLQEGDLDEALKLFEESIDLAMSTEDKYIAIKLHEGVKTQLRLKQDPYMSKRIDELLAQYAMGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP71</accession>
    <name>A0A1E5RP71_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative transporter ESBP6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g731</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP71"/>
    <dbReference type="FunCoup" id="A0A1E5RP71">
        <property type="interactions" value="443"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RP71"/>
    <dbReference type="OrthoDB" id="2213137at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17352">
        <property type="entry name" value="MFS_MCT_SLC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF315">
        <property type="entry name" value="TRANSPORTER MCH2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="251"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="287"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="320"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="347"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="377"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="408"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="465"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="528"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="553"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="588"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="620"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="39"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="671"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="48"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="95"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="116"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="671"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ88658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="731" mass="80948" checksum="94887095147E74A7" modified="2017-01-18" version="1">MSSNSKKDSNHEVSSGALGNDPEILSSDLEIQSVYSNASVGLGDPVPASQTNRLQESNSNEKSVSKRLQKLNTSKSFQSKSSNVSKMFSHRKGSVKHDDNQTEKQLRSAVNLKNESSSKSETDRDLSKLLSRNFEFEDALRLTASHRTKTSAKSKMENLQKNLQNSNELPPDLEATKSKETLFPHGNDKPLQSKSLQKNFQDTGNENEETDQENISNGEEDVQTLEKVFTNHQTNTIDLPPDGGKDAYGSLVAVLLIMFCSWGANASNGIILAFFLNTNKFAGGTKFKYAVISGLSLCIGQFGCPFVSIVVKVFGIKPPICFGILCTFLGYFMGAFATKLWHLFCTLGLLCGVGISFTFLPATAVLPGWFLKKRSMALGTSLVGTGLGGFVFSLSTNALLERDQNQKWALIAQSIVCAGLSTIAVILVKQRNPPPTIPLKEWRVVAAQWSTIFSIKIMKRYITNLIAFWFTLAIFGYNLMILSLSAYARARGLTANQASYLTVYMNVAQSVGRPMIGLSGDRFGRSNITVFLTTIIVTLIFAFWIPAHTNGQLVAFSVLIGLCIGVANVMNTVMIADSVAPKDFLASWSYINIFGSPLFLFAEAIVQALTVPENKSNPYLHAQIFAGFCFFVAIFLSLLIREYKVRNLLKNKLCSTLRDLDNYNQEKKFDAKSSIESQNDKDTPPIDFDDTFQTENTPDEALLVERKENYELLLQRNIRAVIHRAFYPIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVF1</accession>
    <name>A0A1E5RVF1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab GDP dissociation inhibitor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g471</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the Rab GDI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVF1">
        <property type="interactions" value="823"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVF1"/>
    <dbReference type="InParanoid" id="A0A1E5RVF1"/>
    <dbReference type="OrthoDB" id="9446342at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005093">
        <property type="term" value="F:Rab GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.405.10:FF:000001">
        <property type="entry name" value="Rab GDP dissociation inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.519.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018203">
        <property type="entry name" value="GDP_dissociation_inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000806">
        <property type="entry name" value="RabGDI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787:SF8">
        <property type="entry name" value="RAB GDP DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787">
        <property type="entry name" value="RAB GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00996">
        <property type="entry name" value="GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00892">
        <property type="entry name" value="RABGDI"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00891">
        <property type="entry name" value="RABGDIREP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ90806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="462" mass="52053" checksum="AC96A430FAE8EEC4" modified="2017-01-18" version="1">MEENYDVIVLGTGLTECILSGLLSIEGKKVLHIDRQEFYGGESASVNLTELYKRYKPEYLGQHGGKIPEKYGRDRDWNVDLIPKFLMSNGELTNILIHTDVTRYVEFKQISGSYVYHKNGKIYKVPANEMEAISSPLLGIFEKRRVQKFLEWVSKYRDNDTSTHHGLNLDNNTMEEVYYKFGLGKSTQDFVGHAMALYPTDEYLQLPCRPTVERIILYSQSVARYGKSPYLYPLYGLGELPQGFARLSAIYGGTYMLGTPVEEVYYTEEDPTSSSDSTTAKSKKFAGVKTKEGIAKAPLVIADPSYFPDLVEPTGEEVIRCICILNHPXSGSSSSDSVQIILPQNQIGRNHDIYIAMVGESHHVSGKNYYLAIVSTIVETKEPLKELEPALKLLGEIEDKFVGISKLMKPKEDGTKDNIFISKSYDASSHFESMTDDVKDIYFRINGSPLVLKTRQDGETEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVM8</accession>
    <name>A0A1E5RVM8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA damage-inducible protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g461</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable aspartic protease. May be involved in the regulation of exocytosis. Acts as a linker between the 19S proteasome and polyubiquitinated proteins via UBA domain interactions with ubiquitin for their subsequent degradation. Required for S-phase checkpoint control.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Binds ubiquitin and polyubiquitinated proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DDI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVM8">
        <property type="interactions" value="280"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVM8"/>
    <dbReference type="InParanoid" id="A0A1E5RVM8"/>
    <dbReference type="OrthoDB" id="1047367at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05479">
        <property type="entry name" value="RP_DDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14309">
        <property type="entry name" value="UBA_scDdi1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001995">
        <property type="entry name" value="Peptidase_A2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019103">
        <property type="entry name" value="Peptidase_aspartic_DDI1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15397:SF3">
        <property type="entry name" value="DNA DAMAGE INDUCIBLE 1 HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15397">
        <property type="entry name" value="SODIUM-GLUCOSE COTRANSPORTER REGULATORY PROTEIN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09668">
        <property type="entry name" value="Asp_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50175">
        <property type="entry name" value="ASP_PROT_RETROV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A2" evidence="10">
        <location>
            <begin position="180"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="9">
        <location>
            <begin position="349"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="309"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="320"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ90828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="389" mass="43053" checksum="9C7F0114F15D8F9E" modified="2017-01-18" version="1">MXITVNNEANKSIYGPLEFSDDLTLQDMQALLQFEAEYDEKKHELVYDQKVLDVANDGIKTLKELQIKDNSSITIKMKKLDPIDQLRLKLLDDAKTPEILKTEILVDLRGFKQFIEFIKFSPISRSKPLVQVLKNSELMEELAYAKKIDEQMTHAMEYTPETFTTVSMLYINMEINGHPVKAFVDSGAQNTIMSTKLAKLTKLDKMVDKRFQGEARGVGVSNIVGKIHSALVKIETQFVACSFTVLDINVDLLLGLDMLKRYQACIDLEKNVLKFAGIETPFLGEADIPKEPIVEXIAGADVNVGSSAMGPEGEGFNANKRATTTQAKSASSNKQPKQAEGTPLGMPVQFSESSIKTLMDLGFSKEKVIEALKVANGNADLAASILLNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVN9</accession>
    <name>A0A1E5RVN9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g357</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVN9">
        <property type="interactions" value="170"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RVN9"/>
    <dbReference type="OrthoDB" id="4052026at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ91010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="336" mass="37104" checksum="B28C89C6481C5B19" modified="2017-01-18" version="1">MNNRKSIASNDALNRKNQPLKDINNRLVNARPTEPDVFKRLSTIPDSSKKRANHSPSTSASPYSSVQKLSKNNINIKSPSRTAEKENLRPQSTPTSSDVSSSALQTLLKHNQNNGSGFPVSPLRLSPERLANNQRLVPGKGSLGRIRSRFNQPYKMNTILSTPERVGSNVSQLQKVGKPFISPQRVNRTSHSNLSKKKNLLTDLMASQINETEPVNASSPITKSALKQIKDINTQSSSDLESAVLKNPLNKSFSGNTKKSVTFANPPDTLIEKESAPXPAQNSLEMPETILNPIFEDNSILESSDPNQEIMKVLLQILKRQEQIESKLDSVLAEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVP8</accession>
    <name>A0A1E5RVP8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CST complex subunit Stn1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g435</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Telomere</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RVP8"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018856">
        <property type="entry name" value="Stn1_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10451">
        <property type="entry name" value="Stn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="CST complex subunit Stn1 N-terminal" evidence="5">
        <location>
            <begin position="158"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="504"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="546"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="553"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ91020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="788" mass="91127" checksum="4EBE74D0D0B99FBE" modified="2017-01-18" version="1">MEENIVFFSERLQDHIPFNYRKNYIQKYPQIDKPVEDYLTPLLDLTIDFYYPFAFQWFTPHYLGEYARTYIPLPVRLFYQFETYSLKVMGKYVSKLEFKSDEQLTELEHHMKVYFNLFQNDIFHKSVQHSTQDESFSQESNISIEYPEPRFRVNPYKLLFIYNKPIEKVCLLGTCCGCKNWGTDGYILSIDDCSISPVHQLRRDDXCCYVGIDFLMSNGISTNRSDIIGEKFLIYGRVSYSDYDQEIGINVDSITIVASMRQEFEHYNLMEEIAEKLINGEPWFLPRATKNQESQNLEKIVYTFPQYLQSIFDEETTRGKEEHGLVEDEQRSKAVIDQRAWLQQELEITHLNVFQKVGTSVTESESLKTDKMYLGSSLPKHIDQSLYHNAFSHINSQIHEVDFSPVSGHNFSSRFLNDRSHADDEEVDLLEGFDXFSSANETSNLRTPTGKAQANDLVMNKDIADANAANENISKANLAIEPDSSNQTETVEAHEIEKSLNIGKTSXLSPPEKGSMTPKPLIVPKNTTSQLQHSIEKRIITSSPAISLPANEASRSSATPTKVSVTPKEDQDTTFTLDEMYRDYKAEFMNQFMKISLDNRHDVLHNNSFHYSVPALSXKLGNFANAITKKSFERNLEIFLHDQESLKKNYKAMYENNKLVVAQKVSKEFAKENPLVFHTQEDGDLVSLSNERKILVYLIDKIQKMIALRLSNFKITFDLTFCRNIEGLKMVEQDSSLLVNDVGLSMEFIDKMLHYVMKHNSSVRNGIVKKWMITSQENFFEFCIKCSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RYW9</accession>
    <name>A0A1E5RYW9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flocculation protein FLO1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g282</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000002">
        <property type="protein sequence ID" value="OEJ92157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RYW9"/>
    <dbReference type="InParanoid" id="A0A1E5RYW9"/>
    <dbReference type="OrthoDB" id="4036630at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000128">
        <property type="term" value="P:flocculation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001389">
        <property type="entry name" value="Flocculin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00624">
        <property type="entry name" value="Flocculin"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1646"/>
            <end position="1668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="704"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1177"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1269"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1366"/>
            <end position="1548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1373"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1392"/>
            <end position="1526"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ92157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1670" mass="174837" checksum="19A92CD0310B73BC" modified="2017-01-18" version="1" fragment="single">ETPVSRSSSTYYSYWDGTTDSTYSTEVDTITIDGSPVISTVFYVATPEAHGTIERTGSWTGTVTQTYSTSFSTFSGDNGRATVDTYYFVETPITRVTETSYSFWSGSVNSTYSTEVTTNYVFNSSALISTVYYVATPEEQRSFTSTSHWTGLNTATYSSSYSTYSGGDGSATVDTVYFVATPMSRYVDTTLNSFWNGTTDSVYSTTVFTAFNANDSSPYISTVYFVATPEAHGAYTKTTYWTGLNTATYDVSYTTYAGSDGHETVDTYYFIKVPYATHSFNSSSSYVGTSYSFGNVSVLTTFATSLSTFSGNDGASIVSTLYFVAQPRITTTSFVTIPFSGSNVLTLDPTVTSFTNNNGTQIIMTVYTVETPMLQASTVYTSFYEGSARSTFSTRTIVSTFNSSQITDTVYYVAVPRSHVNSTVTSHWSASFASTISTASITFTGEDGIETEELVYHVDIPDQTSFSGSNSSTSLTYSSSSFMSSSQSTVSVHNSSVSPSYVSSSTPSYPASTSGHTIFSTFFSGADVNITKTFSVSTVYLTDSFGSTSDTVIEYYVQTPEIHQVSTTTEYWSEPNFETYLTSYSNYRNNDGTDVAETIYYVHAPYATLASTSTIYWGETYTSVFPIETLTLSGFDGSSTHETIYAVHIPEERTATTYTTRVSGSITITLSSAIKNVTGTNAIPTIVTEYYVGVPSIVPTSFTKASNTSSSTSLSTSSSSTSSSAFSSLSPSSSFASTLTTTLYEGFDGNSTETYSTLTKILTNTNGNPTETILYYYVHTPELHHTSAITDYWYQSEVSTYSTQYNSQVDTNGNTMIDTIYYVHLPYSSKSSFATNYWSETFTSTIPTTTLVSTGSDGIPTYDTIYAVYIPEPHSESTFTTHVSGSVTVTLSSDVIVVTGENSIPTMVTEYYVGVPYERTSIXYVTSTMSISSVITYSTNSAFSTDNEGNVAVTSIYYVGVPYPTTQGVQTITTGWDNTITSTYSTVFETSVDTDGKAVVSTIFYVQVPAVVTTEFVTTFWEGISSVTYSSSTRFISGSDGTPTYDTIYYIAVPGASTQSSWAGYSNSSGFASSFSDTSSSSADSFSGSSASLPFESGSSSLTLSSSLGTDTLSSSTLSSSTLYSSVSSFSDLSSTTLSSSVFSFSDLSSTTYSSGSSDKSLPTLSLSASSTSMVASQSSENESSSYNSVSYPDSTGQSSHESLTSSSTXTESTSSTSSGAQSSTPSLSSXTXGLSSITFGVSSSSVPQSDLVSSSSIIAQSSAIISSFNSSSQGTPTVTSSQDSSSXLSTFSETHLSSTSAQSLTDSLSTDSSFSILTSSEITDSTPTNSYIPPYSVTTTTVTNIYSETTVVYVSEVTFTASPAMTSTTSTNKNAGETSTAESGYESMNTAGSSGSSSSGSSGSGDSAGSSGSSGSDSSNAAGSSSSSGSDSSNAAGSSGSSGSDSSNAAGSSGSGDSAGSSGSSGSDSSNAAGSSGSSGSDSSNAAGSSGSSGSSGSDSSDSPSNGSDAVNDSDSNNATASSGSVEAMDHISSSTSPSTTKKKRRVTVYETVSITSTDYDSIVTQYETKMISFTSTVTVTSRERLSYYPSSTYMASEADTAATTVAQANPTVSGSGSTSSASASATATYSISGSISTGAAYNKYAGSSFASKIASLFTICFVVFFFNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZP1</accession>
    <name>A0A1E5RZP1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cytoplasmic 60S subunit biogenesis factor REI1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g44</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the REI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZP1"/>
    <dbReference type="FunCoup" id="A0A1E5RZP1">
        <property type="interactions" value="898"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZP1"/>
    <dbReference type="InParanoid" id="A0A1E5RZP1"/>
    <dbReference type="OrthoDB" id="19329at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041661">
        <property type="entry name" value="ZN622/Rei1/Reh1_Znf-C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040025">
        <property type="entry name" value="Znf622/Rei1/Reh1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13182:SF21">
        <property type="entry name" value="CYTOPLASMIC 60S SUBUNIT BIOGENESIS FACTOR REI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13182">
        <property type="entry name" value="ZINC FINGER PROTEIN 622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12756">
        <property type="entry name" value="zf-C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="108"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="303"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="303"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ92460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="452" mass="52409" checksum="985D37CE796F90CD" modified="2017-01-18" version="1">MSATYTCNSCLITFSTSLDQREHMKGDWHRYNLKRRVAQLPPISEQTFNEKVNVLGNGTTSESPESARDGKNKTLTKKEMRRLEKEKLLAKKKKLLEIAKENMLKNMQTTESQSEAQTDVPAQTASADVPTQTASTETLDTELNKLHIADNKDTVLESKQEDTELSEEQLAEQLMEEKLKNKVDFLPEQCLFCTKRTVFPSFEANIEHMYAHHGFYIPEQKYLVDMRGLVKYMGEKIGIGNLCLCCSYQGRSVQAVQSHMLMKRHCKIPYESEQEKLEISEFYDFSSTYNAFGNNTEKKENLEEQTLEENDGEWEDISEEDDTEDYEIIEESQSSSKEGKGKGEDEEDEEEIPEEYLYNDGYNLHLPTGVKVGHRSLQRYYKQVTKPERVLTEGQGTVVAAETRHFVALMDKHEINSTKRVWQSQIKDRKRDDKRRAKFINAKEHYRDQLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZW1</accession>
    <name>A0A1E5RZW1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DnaJ-like chaperone JEM1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g18</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZW1">
        <property type="interactions" value="96"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZW1"/>
    <dbReference type="InParanoid" id="A0A1E5RZW1"/>
    <dbReference type="OrthoDB" id="1726119at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:protein-folding chaperone binding"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051727">
        <property type="entry name" value="DnaJ_C3_Co-chaperones"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44140">
        <property type="entry name" value="LD25575P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44140:SF2">
        <property type="entry name" value="LD25575P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184960" evidence="5">
        <location>
            <begin position="23"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="6">
        <location>
            <begin position="470"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="417"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="527"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="417"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="438"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="527"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ92285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="547" mass="63863" checksum="B92A1BA1E8B8AD92" modified="2017-01-18" version="1">MIXRTLLSTFCYFAILVSSSVCDKQETYKALSITIPASTIDYQKIVSYLEQNXCARSDDMDVLKFYEENLYQLGLKKIXEDEEYEAIQHFQKISKTHWKNLSASRLASLNVKYGIGNPSEVENLPINERISISPYSQAYRIEFINHLLYEIENFENESDVIYNVQQIVDSYQIILDKYRKSLSLGQRVEMFSKISILQTFILNNCKAALKALKQCYDLDMDNKVCTSFLKLQNKLSKXTKQADGYLYFDSQFDLPWATNYLKTIENTKKPLIKDVTGALSLEFFKTFPEMKKXYKDGSDLPLVKSLYVLSTIAHDTQXVLEKKQKSAESPYLKRLIDVKKHYNEEELRILWEELSPHXAMKILLKTDIKQTQKXLKERDNCWDAQVHNTNSPWFHKVLNALHAKVVKIAQKEQEQQQDFFRKFQQQQQQQQHHHHFQGGRQFFHGGPGGQQQHYQQQQAPPPSGVNPEKDYYKILGVXNGASSKEVRSAYLKLIKQNHPDKQKTQEEKEKVESLVHDINEAYEVLNDEEKRKEYDGARSSHGRGRYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S033</accession>
    <name>A0A1E5S033_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Sec sixty-one</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g58</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5S033">
        <property type="interactions" value="179"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S033"/>
    <dbReference type="InParanoid" id="A0A1E5S033"/>
    <dbReference type="OrthoDB" id="420669at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="entry name" value="SecY subunit domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030659">
        <property type="entry name" value="SecY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="SECY/SEC61-ALPHA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="Preprotein translocase SecY subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00755">
        <property type="entry name" value="SECY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="294"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="416"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ92446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="483" mass="53748" checksum="05F94C305B63E3A4" modified="2017-01-18" version="1">MAGFRILDIAKPLQSVFPEVYFPYEAAPFDEKVIYTIFTGLIYLFLQQLPVAGYTPQGTIQEADQFYYLRNVFAMGAPHSVLEFGLFPIISSTIFMQLLSGLKLFKINYKKELERHRLQTLNKFFPLLITFILTNLNIFVFKYYGSDLETTFLSKILINVQVLIGTLLSILFVEVIDKGYGFQSGVLCLQFMSYTTTFVNNILGVSNMPIDDKGNTEPQGSLVNFIGAFRNPNNTWTGALVNAFSRSYLPNVTQMVFIAGIGALVIYWSHYRYELTIRSTKMKNVTQVFPVKMLYTGGLSIFFTYSIIGFINVFLSTILTLVAPKFVNVDNNLLVLSFPFNLLLPPRSVFPDLLPNNILQIIIFPIFFIGSCIWFSYNWMLLSGSAPSDLASKFKEQGITIQGKRESAVKQDLNKLIXPAAISCGLVLGGLVAVGELIGLKGDAAGMVLAVASCFSLLELIIYEFQQTGGKSMFASVLGGAGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S039</accession>
    <name>A0A1E5S039_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g191</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the MCM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5S039">
        <property type="interactions" value="1121"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S039"/>
    <dbReference type="InParanoid" id="A0A1E5S039"/>
    <dbReference type="OrthoDB" id="1882346at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006267">
        <property type="term" value="P:pre-replicative complex assembly involved in nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17754">
        <property type="entry name" value="MCM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003812">
        <property type="entry name" value="MCM / cell division control protein 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008046">
        <property type="entry name" value="Mcm3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056575">
        <property type="entry name" value="WH_MCM3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF46">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23191">
        <property type="entry name" value="WHD_MCM3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01659">
        <property type="entry name" value="MCMPROTEIN3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="13">
        <location>
            <begin position="369"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="198"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="603"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="764"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="839"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="604"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="777"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="839"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="912"/>
            <end position="923"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ92365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1008" mass="111834" checksum="C711773CEF6B1340" modified="2017-01-18" version="1">MTDNSDSLFNDKVRRFQDFLDSYSNYKSDIKSILVHNAELELXKQKPNINYSTSLYEDEGRKNAEKEDEELPQRLIISLDDLREFDRVYWNGILHYPHEYIPPAEHSISLLAMTEQPTGTLRPRHQTNVQWTLGFKGSFGANTLTPRTLSSKYLNKLISIQGIVTKTSLIRPKLFKSVHYAPKTSRFHYRNYKDATTSLNTPIPGGASSTGNNAENGDASTGNNTSQDQMALTSAIYPTEDTEGNKLITEFGFSEYKDHQRITIQEMPEQSPAGQLPRHLEVIIDDDLVDSVKCGDRVNIVGVYKSSGGGGLMDKKGLMGLQGFKTFILASTVWPLHARSTGVGAMENVSAKDISKIIKLSNNYKNENIFNLLSRSLAPSIYGHESIKKAVLLMLLGGVEKNLENGSHLRGDINILMVGDPSTAKSQVLRFVLNTASLAIATTGRGSSGVGLTAAVTTDKETGERKLEAGAMVLADRGIVCIDEFDKMSDIDRVAIHEVMEQQTVTIAKAGIHTTLNARCSVIAAANPILGQYDVNRDPHHNIALPDSLLSRFDLLFIVTDNINDIKDRQISEHVLRTHRYVPPGYLEGEPIREQINISLGVGVEDDDNGADDDEDDDDDIDDHNMDDIIDXEFHADNMSSVFEKFNPLLHAGAKLAVNNGDHNGTNVPLIVSIKFIKKYIQYAKERIVPQLTQPAIDIIVKAYSDLRNDITNKKLPITARTLETLIRLSSAHAKVRLSQLVEAEDATVATQMLRYALLNEDATTEKGNGIPSPQKKSPRKKSPRKKLKPSPKKKTTKKSTAGTEEIASEHEQDDEEIVTQGRTDLREAMETADLEERLEKGLRISPRKQEQLQARRAVLSPRKDTNILESPSKKGRSSRFGQTDDASLRSDPYSGDENVMPSSPLIPVDVPFEDPEEDDDHDSESAEISQARLSLFNSVFAPLLSDSSLFVDDYCEVEKLFEAVNTHLKNKDSDAEPFSQGEYLAALEALQATENIMLADGLVLRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S053</accession>
    <name>A0A1E5S053_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fumarate reductase</fullName>
            <ecNumber evidence="6">1.3.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g203</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Irreversibly catalyzes the reduction of fumarate to succinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>succinate + NAD(+) = fumarate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18281"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.3.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="6">Binds 1 FAD per monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.6" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5S053">
        <property type="interactions" value="77"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S053"/>
    <dbReference type="InParanoid" id="A0A1E5S053"/>
    <dbReference type="OrthoDB" id="10254877at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016156">
        <property type="term" value="F:fumarate reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.700.10:FF:000007">
        <property type="entry name" value="NADH-dependent fumarate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050315">
        <property type="entry name" value="FAD-oxidoreductase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010960">
        <property type="entry name" value="Flavocytochrome_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01813">
        <property type="entry name" value="flavo_cyto_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43400:SF7">
        <property type="entry name" value="FAD-DEPENDENT OXIDOREDUCTASE 2 FAD BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43400">
        <property type="entry name" value="FUMARATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0274">FAD</keyword>
    <keyword evidence="2 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="7">
        <location>
            <begin position="8"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="8">
        <location>
            <position position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00050832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ92353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="489" mass="53685" checksum="02E39B2165DFDC9B" modified="2017-01-18" version="1">MSASQLPVVVVGSGLAGLSAATQLITKYKIPVVLIEKADKIGGNSVKASSGINGVQTKTQIENYHITDDNADXFYQDTLKSSKNLGVPKLMEKLAKDSTKSIEWLQNEFQIKLDLLSQLGGHSKMRTHRSSGKLPPGFEIISTLQKNLEKIXDTTPEMIDIKLNSKLSNIKINSITKQIEYIEVQNTKDASASSSTNNVQKIYTNVLVLCTGGFGFNKEMIYEVTKSKFISELPTSNGQQTLGDGQQLMAQLGAELIDMDQIQVHPTGFVDPTDRKSNVKFLAAEALRGLGGILVNPVNGQRFVNELDTRDHVTDAIFNTKMPDNVALLVMSEKVYEVYSNNMNFYIFKKLIKKTTIGDFYXEHDVAGKWGSQETFISQLIEYSNESTQDKFGRDVRVNSFGNNVNADTVIYVGEITPIVHFTMGGVKINEKAQVLNNKNQLVAHGLYSAGEVTGGVHGANRLGGSSLLECVVFGRQAADSIAFEFSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S0A5</accession>
    <name>A0A1E5S0A5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g158</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5S0A5"/>
    <dbReference type="InParanoid" id="A0A1E5S0A5"/>
    <dbReference type="OrthoDB" id="3969899at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ92403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="126" mass="14375" checksum="B5E16236C2C80DD7" modified="2017-01-18" version="1">MMNRQLTAFARVAPMSKVVARFPIRQFHTSLLHMQSYQKWADLDKAGKQNFIKSFVNLHIEKHPCSKSNVSYQALANDMEEYDDTPYIFGILYDEIRAVAQKESVHNTDGSGFTGDPDFAKLLSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R023</accession>
    <name>A0A1E5R023_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">DNA polymerase eta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g4650</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000017">
        <property type="protein sequence ID" value="OEJ80241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R023">
        <property type="interactions" value="618"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R023"/>
    <dbReference type="InParanoid" id="A0A1E5R023"/>
    <dbReference type="OrthoDB" id="5723at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009314">
        <property type="term" value="P:response to radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.60:FF:000008">
        <property type="entry name" value="DNA polymerase eta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.100">
        <property type="entry name" value="DNA polymerase, Y-family, little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036775">
        <property type="entry name" value="DNA_pol_Y-fam_lit_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052230">
        <property type="entry name" value="DNA_polymerase_eta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041298">
        <property type="entry name" value="UBZ3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45873">
        <property type="entry name" value="DNA POLYMERASE ETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45873:SF1">
        <property type="entry name" value="DNA POLYMERASE ETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00817">
        <property type="entry name" value="IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036603">
        <property type="entry name" value="DPol_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100879">
        <property type="entry name" value="Lesion bypass DNA polymerase (Y-family), little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50173">
        <property type="entry name" value="UMUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51907">
        <property type="entry name" value="ZF_UBZ3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UmuC" evidence="10">
        <location>
            <begin position="26"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="UBZ3-type" evidence="11">
        <location>
            <begin position="572"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="547"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="552"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ80241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="670" mass="76770" checksum="B4DF361CD68AC15E" modified="2017-01-18" version="1">MSQFTWKDLIDLNSKEKSYASGLSCIAHIDMNAFFAQVEQRRCGFDDDAPVVCVQWNSLIAVSYPARKYGINRMDNVDSAKEKCPNVLLCHAAVFKKNEDFWEYKPIGVRLNPMHYKVSLEPYRREGRKILKVFKKYTDLVEKASVDEAFIDLGKLVFKHILLSSDNDXFKPLKDMFMKGEYNVHDPLPSVEEFDLAQIKTEGNIINPSNQPLLKDWNDVVIAIGSQITLQIRQDVKATFGYTTSGGVSHSKSVSKLASDFLKPDAQTIVFDRLVSDFLDVGEFEITSFWTLGGGRGKELMDLLGLPKDQGHSIKYLRENWSQQSELRAYLELKYKEYSLFKKYGFHFEDCXAISEKLFKLSRGHWKEVVNPKPVVKTMMVNKNMRGNSCNELKDCFSWFKVFSAELAGRIVELEKEYNKIIIPKTISVSLRGASKHQFPKHSKSRSFVVSSYGHSNDTDEASAKYIKKLAGDIMTQASRLMEELDSNLKFTIYPLTNLSIGLSNMAIVEAGSTIFDMFKNHTHEGHSGKTNQHQLVPSAQETFSNVKQENEAENEAENEAENEAENEXDNENENFYYCEKCEKMIDKKVENLSYAEHMDFHYAQEFSTGLNGYDREQDYERMSYAEKRLLFGSAERSNKAQKKVTKTTENKAKPVKNNILNFFNAKKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0E2</accession>
    <name>A0A1E5R0E2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Target of rapamycin complex 2 subunit sin1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g4519</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0E2">
        <property type="interactions" value="256"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0E2"/>
    <dbReference type="InParanoid" id="A0A1E5R0E2"/>
    <dbReference type="OrthoDB" id="241990at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031567">
        <property type="entry name" value="CRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008828">
        <property type="entry name" value="Sin1/Avo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031313">
        <property type="entry name" value="Sin1_PH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056385">
        <property type="entry name" value="UBL_AVO1/Sin1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13335">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT MAPKAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13335:SF1">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT MAPKAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16978">
        <property type="entry name" value="CRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16979">
        <property type="entry name" value="SIN1_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23164">
        <property type="entry name" value="UBL_AVO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRIM" evidence="3">
        <location>
            <begin position="695"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="domain" description="AVO1/Sin1 ubiquitin-like" evidence="5">
        <location>
            <begin position="882"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="domain" description="SIN1-type PH" evidence="4">
        <location>
            <begin position="1088"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="112"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="273"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="630"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="669"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1046"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="184"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="278"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="395"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="672"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ80356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1199" mass="134855" checksum="230D03B27E06B571" modified="2017-01-18" version="1">MDSIYAINKLRAGFLKASSDVANARILAPYKIDELQTDEAFRSQIKQLYFDKDKDIDYLTRIKSPPIDDNYFVVVVQEDPSKEAVKRADSTSLYDQSQISINENVLDFDDGNSSSHSVXDHKNVDNNINRKVSGFSAGGSSLKTRRPSTTSTQSSGNLRDDATMHTKNSVLTKKSKTPSTFSLRKSDSITQDDKKSLTNKKSRIKSKLSRLSLSKIFKHDKRHALGNGXAHFLSHSSDYDDEHHLDDDSASTNLKIRSLSTQRSDDKELEFYDRADNIFEDEDDDDDDDDDDDDDDDDDDDDDDEDAIGFDRTEKNNFRKGKGKPSYGPNTKAHGSLSAKNESASTNTFNNHSRVTSDSYIDDQTLHSVNNMKSGTSSSIRMGQSLKNFPDGEATNKQSVSSLSSQAEAKNTKKPTIIKGLGALNPIGNGINVASKVNGNTGNPLSGSEHYGSLIQGSNAEISNQNKEDDSFDIDSFVNEDELGQLDLDNVNTLAQEHKIYDTIAEDSLNNSLSSSFASVQHNKNIYENIDNIQQKTHRTLNESQKNYYSDLASSYDDSLMDSDYEDTQDPYVSKNDSKMLGHSIPSSKDIYDNKLVTERFGSMSXYQSKYLPTSERSLANHKRTLSSESRLAFGKAHHTKPLLPKDSKDRLKHSGSFVKKGLSQFLPSSSDAERHTEPISFEKKSSVSSNPHSSQLTNMFKVTDENDETKLSHFQYVSASQVQPKDRASLLVYIQDYDKYKHNGFVVKVRKDATVYETINYILFCFIAIEKLSPEKNNYSNPNEFTLRIVDEDGEPFEDNFGILDRESKIGAIFDEEVVLCRIHDKAQVARNASTAPDFGFVKNKQTPLVGAADAPPVQQLSYYKSLTTGTGNPELVQXSTIDIKVYKYPFAESAMINSTTVTVLLTSKINDILTKYCKLKNLDPTEFYVKKFKDNYFYDLKDNIISLDGQKQVEIISRKKGRQLRLTKYCSDNSQNRSVLPTIQSNEAAPFTLNTDGNFLPVGDITTKLKKSSSQQNGAKWGHSAEQKNTQNSFLYKIKNPSKTSIQNDPTSAGPASGGGNSSSSKNFLFTPTSYAQNKNFLSTDAYFKYTVYRRQQKSLISNKHERTLAIDGEYVYIIPPDDEHHWYEHHSIKTKSFHVSQIILVKKSSKNDLYFKIIISRQQVRKTYYFEALNAEERSEIVEKLQALTSVYNMNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0E8</accession>
    <name>A0A1E5R0E8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="9">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g4515</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="9"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0E8">
        <property type="interactions" value="990"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0E8"/>
    <dbReference type="InParanoid" id="A0A1E5R0E8"/>
    <dbReference type="OrthoDB" id="4310724at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17946">
        <property type="entry name" value="DEADc_DDX24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9 14" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="13">
        <location>
            <begin position="214"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="246"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="507"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="92"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="8">
        <location>
            <begin position="214"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="172"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ80357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="820" mass="92586" checksum="FBF9E15D91444BA1" modified="2017-01-18" version="1">MSVQNKNKRNSRPTGPTFSQKKKQKKSASSSSLHKNKASNDYQVVNLNALPWKKIEINENEVTNSDFGGFYGLEEIDGVGLKEIDGKLQFVSTKAPTDTTAKDESDGKETKVPPHTMDDIEFDEDATMEDLIEFKNLDDIQPGELSVASGSDDEWDASDAEEIQEKLQSEVQGEEEESDTNEDPDRDLKQNTFNLLADVLDIEDGDQPPMLEEWNALNCHLSYTTMQGLANLGFTKPTEIQAKCLPLAINEHRDILGKASTGSGKTLAYGIPILEKLVSKVKTTTSASTKNGKKKDIEDDIPVAVIFTPTRELAHQVTEHLSNLAKHLIKNNPYMILSLTGGLSIQKQERLLMKYKGNAKIVVATPGRFLELLEKNQQLIQRFSRIXYLVLDEADRLLQDGHFDEFEKIMKFLSRSAKKINPQKYWQTLVFSATFSLDLFHKLDNTSWKNLKSKQNDQDSEMEQVVKHLMSKIHFGSKPLVVDTNPEQKVASQIKESLIECLPTERDLFVYYFVTMFPQTSLVFCNSIDSVKKLNSYLNNLKISCFQIHSSMTQKNRLKNLERFKEISKKNTVLGKPTVLIASDVAARGLDIPNISHVIHYHLPRTADVYIHRSGRTARGENSEGVSVMICSPQEAMGPLRKLRKLLHSEKPSDIVNAKGNPVTNKKKWQKDVKPLVIESDILTQLKKRSSIANELANHEIASKSLSKEENWLKQAADDLGIDLDSDEEDRDVILAKNKLKKVNKTLDKNEVNTLRYQLNVLLQEPIRKDLRRSYLTGGAVNLADFIVKNKGHNNIIGHEKTDALEQLKKKGKNKNKSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0I7</accession>
    <name>A0A1E5R0I7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4542</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0I7"/>
    <dbReference type="FunCoup" id="A0A1E5R0I7">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R0I7"/>
    <dbReference type="OrthoDB" id="3972117at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038882">
        <property type="entry name" value="Rcf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39153">
        <property type="entry name" value="AGR244WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39153:SF1">
        <property type="entry name" value="AGR244WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="118" mass="13514" checksum="F258EC1E0091AD6E" modified="2017-01-18" version="1">MSVKPVHYDKDSIKELTKELLVAGTKGAVQGAIISITTGLLLKRFSPTYRNVRNQVKVFYHSAWIASGSTFQCDKQLIKFQERYYQSELDRRSRILDEAAERGIFLEEDMVSKSVIPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0K2</accession>
    <name>A0A1E5R0K2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cAMP-dependent protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g4401</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.11" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0K2"/>
    <dbReference type="FunCoup" id="A0A1E5R0K2">
        <property type="interactions" value="964"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0K2"/>
    <dbReference type="InParanoid" id="A0A1E5R0K2"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010607">
        <property type="term" value="P:negative regulation of cytoplasmic mRNA processing body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010737">
        <property type="term" value="P:protein kinase A signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05580">
        <property type="entry name" value="STKc_PKA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF73">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="171"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="14">
        <location>
            <begin position="426"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="87"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="441"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="90"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ80425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="486" mass="55691" checksum="0A0D39B05D981EE9" modified="2017-01-18" version="1">MTNYPGEKTLEVNSSEVNSESPSSVVSGMTIHTNKNSIVDSQRSDNASFQNHDLENEIINDKSNITNSFISGVGNGSASTNEQTIHSEVNNNNNNNNNNTTTQNTDFNNNNSDISTINNDENASHNNDDSVQNDSTISKPIPVSSSLLSLQPKFVKEQCRETKGRYHLSDFQILRTLGTGSFGRVHLVQSNHNYRFYAMKVLKKSKIVKLKQVEHTNDERTMLSCVSHPFLIRLWGTFQDYYFVYMIMDYIEGGELFTLLRKSQRFPIPVSKFYAAEVILALEYLHDMDIIYRDLKPENILLDRNGHIKLTDFGFAKYVPDVTYTLCGTPDYIAPEVVSTKPYNKSVDWWSLGILIYEMLAGFTPFYDANTVKTYDNILNLPLKFPNFFPDDVRDLLSKLIQRDLSKRLGNLQNGVQDVKDHPWFAEVIWSKLLNKSIETPYEPPIQPGQGDSSQYDKYPEDDEDIRKYGSQLDQPDPYRYLFKDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0N0</accession>
    <name>A0A1E5R0N0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Vacuolar import and degradation protein 28</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4547</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0N0"/>
    <dbReference type="InParanoid" id="A0A1E5R0N0"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034657">
        <property type="term" value="C:GID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038739">
        <property type="entry name" value="ARMC8/Vid28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15651">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15651:SF7">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="946"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="950"/>
            <end position="977"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1134" mass="130256" checksum="61F84EF2EE30A4A3" modified="2017-01-18" version="1">MTSGCLKSLQKIKRKIISSSESFRARYLIISCLPVVELNKEDHTFIEENDLLLXDELLDTVSRFFSTNQHENCMISHQECFLILEILLMLFHIDTTNYLPDITALWLKLANCRIGYTQSSENFCASKSLRLCEKYYECLNKGLSVSYPMIFKVALQEAPYSTQHQHQYKNSGLTENGMHFDEEKDLPFTQNGLAIVKKTHQVSKDWVSNFRLVLQKDLHFLFMHCYALVPLGVLQACISIVDLLIQLDTVFENQNFLVLERDILQRLKSSKLYENAVFIEAEKFLVKLIVHLSFKPSAQIFNTEEEKFVLRIYNMDDTAAPFAVARFLAYCCIPEKSFYSMIEDQIYKYEVPYWYEPYKVMLQYLENIKACQQLSSNNVILQQSNGNTFSGVIAELTSKXXTPXNALDKQTAGSDKTELKGQTFNLQNTKKAFSDATLNDILSDKFNERLFLKLQYDFKNLIRLENPNRGSYLALSQLLDLFTTIAEDGYRKFTLLKLDGLFEDERSNLIKALXKMLDEHFVDIETKEHRTLKDLREEDKNQDQIIKQSSKILYRILKDEGETKFKGVFNRNNQYEYLTNEQSIFYISCRPSPLMQYFKNYLIYHTANIKQLNSKDLGHNYPKVFSSDLAVSMCKLLRVMSRSINSLKVYFIDEDFSKVLLGLIEQDLKLISQFSAQNNYECEACSNELAVLQEVTGLVSNLAIDFSPLKSSLIKFGLLDLLNKLLDTVILQEQKNLGMANSTKNASLAFIVLRVLRNLSFALAFVTSKTVSGHQKPLNDFDLTKILFFINSENIEIQKEVFYFLSNLTCPNCGFDIDKLLKLFLSSSDTNSHKKTCYFFQFLANKLTKIRANNDQTAKKIVSPILQIILNISLHDDDFKKSMILQARELMTNIKYILEDTSMTKSGKNNEEPSVKEICLKILKSLLEFNSDAQFYKKYMINKRKYSGSEGEEEKEEEEEEEEEDLTDDEMEAEENDEHSILSGYAENAISDEEHEDWDAQVNYSGSQSGDLNSRWTPPSQFGEGNMDGASILFTAAQEYLSESQTGSSSSAPLWASRTHAATDNNFFASRHAKNKFLFFEKRKEEILGLGFAIVLKSLLMTDSADPGLAPIVNILISKLDIAPRRPRLSSSDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0R3</accession>
    <name>A0A1E5R0R3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ammonium transporter MEP3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4591</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0R3"/>
    <dbReference type="FunCoup" id="A0A1E5R0R3">
        <property type="interactions" value="355"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0R3"/>
    <dbReference type="InParanoid" id="A0A1E5R0R3"/>
    <dbReference type="OrthoDB" id="534912at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029:SF4">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="267"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="7">
        <location>
            <begin position="16"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="485" mass="54176" checksum="1E8EBA7E90316E25" modified="2017-01-18" version="1">MSELQKDPYALIQMQFLFVGCILVLMMVPGLGYLYTGLTRATNTRKMIASSILVSTVGSLQWYFWGYSLSFSSTSNNKFIGDLHNIVYYNMFSGFTNSIESNIQNYTELPFSIFQGLFMIVTIAILSGCVNERTNYLPFSIFIFCWCTVVYCPCCYWIWNSNGWAYQWGVLDYAGGFIEITSGLCGFIYPIFVFKKKQKHEILISQRASISNVTMGMMFLCIGWLGFNTCTTVDPSDRSFVAFMNTILSGLACFITWSALEIFTEGQCTYVGVCSGIICGLVAATPSSGMIQFWASLIQGVVTAVCCFHAAKFKFLVGIDDSMDILAEHFVAGVIGLLFNGLFANDWIIALDGVTAHPGGWVNQNYIQLAKQIAYIAAVTGYAGVVTIVLLFVIDKIPGCQLFTDYYMNENDMDDEENNLNETDSDLIRNASSDKIIDSVELSNLDNSNSSSMPDILVMKTRQDHEGLERIKSNYTMHPHQTFTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0S2</accession>
    <name>A0A1E5R0S2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Exosome complex component RRP46</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4614</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNase PH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0S2">
        <property type="interactions" value="280"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0S2"/>
    <dbReference type="InParanoid" id="A0A1E5R0S2"/>
    <dbReference type="OrthoDB" id="27298at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF1">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0271">Exosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="6">
        <location>
            <begin position="5"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="248" mass="27479" checksum="AC662572F9327A86" modified="2017-01-18" version="1">MSFLKLKTSLLENVDGSCELSTVNSKVIVSVTGPIEPKQRQEMPQQLALEVIIKPSIGIGPTTREVLMQDKLSQVLQSCLCNYLYPRKLLQVTLQILENPYDNKTGGISGTSGNSYDPVLSALCDLNSCINATFLGIVDSAVGLKSSFVSTLIGVSKQSNELCNISEQSAEQIISTLEYFQSLHLXVMEIIDNKCANVLLIDSTGSFKDEDLLKVLQQAEFNCLETFKNFRSVIKNKVQKDFMWKDIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0T8</accession>
    <name>A0A1E5R0T8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g4421</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0T8"/>
    <dbReference type="STRING" id="56408.A0A1E5R0T8"/>
    <dbReference type="InParanoid" id="A0A1E5R0T8"/>
    <dbReference type="OrthoDB" id="1612078at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021102">
        <property type="entry name" value="PNGase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056948">
        <property type="entry name" value="PNGaseA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31104">
        <property type="entry name" value="PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL)ASPARAGINE AMIDASE A PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12222">
        <property type="entry name" value="PNGaseA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A N-terminal" evidence="3">
        <location>
            <begin position="170"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A N-terminal" evidence="3">
        <location>
            <begin position="446"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="759" mass="86661" checksum="7FE268822AEE12D4" modified="2017-01-18" version="1">MGTRGKELSLYHALHFGLKTENNVNTEENPTEEQLDSTMSEKLTDYSDESDAEYSDAAISEKELLFPTPLTSSITGDLEKQQQAFKKSTGRLHSKKLITKLVFGLIICLLLFFKGFFFNRQCFQSALSNSKDFSENEFPHLPGLPSKKNESSFEITLPAKHSAPPVRVDNLMTHKFADTWGKPKVFHYKPWSAVEEYNFVELEMKTSIEGTQYDRLVHIFVQNITVWRSSTVEPLNRELTKSKSYKDITKYISLFKTNETLEFKFQLDNLVTAKLDGVFDVELNIRYFNLREGNQEHALVENTGCHMNKNRNSMIKNALLHHVNPYSSPDHMEPVVTNRKVSPLMYFPHSQTQRPYSHPLKPFFKKTHQPKLLSHIAVELFVSGNAAEEFWYSNVIDKYAGKFDQYGHSLLGHGPMRLLNVFLTNVSSARTFETSHEDQNKQTFLVHQSIPPPVIFTGGISPALWKPVVSIGAFDLKPIDIDLTPYLKTMLANAEQEWFLEFEVVSANGDTEYRDNVGNNWVISGNVMAWDAIKDPANDIEYIGDTKTTTSNNFIVENKEGEDFLKQNVTSKSILVHQHNILFQDQNYNLTIHHINKLVSNQTYKDFADKQYNTLSILSEKMFVLSNDKGSVHINGGDKQHWALVANVDTLETDEELAELSYKANIVFDYSRKVFMDESNEKLVFSNKEQDEIKNKNLLYHVTASQVGSSKYTLSPKGNHGTGNGVHSVAVTRNWPWTESYKRSVIVKNNKAVIDVIKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0U7</accession>
    <name>A0A1E5R0U7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 16 17">Phosphatidate cytidylyltransferase</fullName>
            <ecNumber evidence="6 16 17">2.7.7.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AWRI3579_g4482</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate + CTP + H(+) = a CDP-1,2-diacyl-sn-glycerol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:16229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.7.7.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 16 17">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 16 17">Belongs to the CDS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.41" evidence="6 16 17"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0U7">
        <property type="interactions" value="738"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0U7"/>
    <dbReference type="InParanoid" id="A0A1E5R0U7"/>
    <dbReference type="OrthoDB" id="10260889at2759"/>
    <dbReference type="UniPathway" id="UPA00557">
        <property type="reaction ID" value="UER00614"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004605">
        <property type="term" value="F:phosphatidate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016024">
        <property type="term" value="P:CDP-diacylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000374">
        <property type="entry name" value="PC_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016720">
        <property type="entry name" value="PC_Trfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13773">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13773:SF8">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE, PHOTORECEPTOR-SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01148">
        <property type="entry name" value="CTP_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018269">
        <property type="entry name" value="PC_trans_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01315">
        <property type="entry name" value="CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 16" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12 16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 16 17" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14 16" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="15 16" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="83"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="29"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="29"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="56"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR018269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OEJ80512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="464" mass="53084" checksum="D6AFE7A9E681BE73" modified="2017-01-18" version="1">MPVEEKEDQQQLRPVRTSKEVFKPKVHHSDDVIVSDDKMHDQQSDDIQSSPSAKVPAEKEVDXKPLEDPTHSNKKQTFIVRTIWTFVMIFGFFVILGSGHIWCIALIIFCQITTFKECIGITHLSSQERNLPLTRTLNWYFLFTTIYYLDGLSIAQFFTKYYIKFPILNWLTLNHIFISYCLYVLGFVLFVSSLKKGFLRFQFSSLCVTHMVLLLVVFQAHLIIQNVINGLFWFLLPCGLVIVNDIFAYLCGITFGKTKLISISPKKTLEGFLGAWFFTVLASLILTKLLAGAKYMTCPVSDLKTNVFTHLACETNPVFIPQRMKLPPIKIFENLNIQSITFKPIYLHAVVLATFASLIAPFGGFFASGLKRAVKVKDFGQSIPGHGGITDRVDCQFMMGSFTYLYYETFISEKRITVFTILSSIIMNLNERQILQLIRELNLLLYANQIITQETFEKLAALYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0V7</accession>
    <name>A0A1E5R0V7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Beta-glucan synthesis-associated protein KRE6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g4562</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SKN1/KRE6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0V7">
        <property type="interactions" value="198"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0V7"/>
    <dbReference type="InParanoid" id="A0A1E5R0V7"/>
    <dbReference type="OrthoDB" id="412647at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015926">
        <property type="term" value="F:glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006078">
        <property type="term" value="P:(1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02180">
        <property type="entry name" value="GH16_fungal_KRE6_glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000140">
        <property type="entry name" value="Beta-glucan synthesis-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005629">
        <property type="entry name" value="Skn1/Kre6/Sbg1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31361">
        <property type="entry name" value="BETA-GLUCAN SYNTHESIS-ASSOCIATED PROTEIN KRE6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31361:SF1">
        <property type="entry name" value="BETA-GLUCAN SYNTHESIS-ASSOCIATED PROTEIN KRE6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03935">
        <property type="entry name" value="SKN1_KRE6_Sbg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="305"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="11">
        <location>
            <begin position="348"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="224"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="88"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="232"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ80539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="785" mass="87453" checksum="34FBC9FE3A8B51C9" modified="2017-01-18" version="1">MSSHNNNSNNNRRVLTGDIFASPDDSSATNLLEHEGSQPYQDRTVFEGSDEEEDDDDDDDDDENEGNDSNPQKEFYDVHKRKSNNPFLDSSAESSYEYSDDSLNNNNNNNKGKGKNKNNSSSDKMDYKGYYASKLPNNDSRNNNNNNNNNNNNNNNNNNNNNNYTGDKKVSYAGIVPPYQAVDRYPLGTSRVGSMMQHGSVRSGMSNMIKDYPGXSTXGGFAVPQTHHESSDGNTSTFSRSTSSQNLVSDQDFSPFGGFPASSFPLLMDEKEDDDYMHNPDPEEEARLDKQRFYLDFKHMDKRSFGGFAAIVCLLLGAGCVFILLPILTYTGVVQHGSAQYYTILSLYEYPQLSSIRTSLVDPDTPSSAMSAKAMDGSYWKLAFSDEFNANGRTFHDGDDQFWLAPDIHYQATQDLEWYSPDAVTTENGTLKLRLDAYENHNLFYRSGMVQSWNRMCFSQGKIEISANLPNYGQVTGLWPGMWTMGNLGRPGYMGTTDGVWPYAYSSCDAGITPNQSSPDGISYLPGQKLSSCTCDGEDHPSPGVGRGAPEIDILEGEVSTDLYEGVGLASQSLQVAPFDIWYIPDYNFVEIYNFSVSSMNTYCGGPFQQAISAVTTLNTAWYEFGLDAGYYQTFGLEYLNDEDTGYITWFVGDAPTWTLNSKTLHPNGNIGWRQISKEPMSXIMNLGISNSWAYINWQSIFFPVTMSIDYVRIYQPNDSISITCDPDDYPTYDYINDHPKAYQNPNITSWEAAGYEWPSNQLTGGCSSKQCSKGSDGSITCKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R180</accession>
    <name>A0A1E5R180_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA exonuclease 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g4518</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Exoribonuclease involved in ribosome biosynthesis. Involved in the processing of ITS1, the internal transcribed spacer localized between the 18S and 5.8S rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the REXO4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R180"/>
    <dbReference type="FunCoup" id="A0A1E5R180">
        <property type="interactions" value="819"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:nucleic acid binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06144">
        <property type="entry name" value="REX4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000007">
        <property type="entry name" value="Interferon-stimulated exonuclease gene 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="REX4_DEDDh_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF45">
        <property type="entry name" value="RNA EXONUCLEASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exonuclease" evidence="11">
        <location>
            <begin position="116"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="44"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ80323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="287" mass="32312" checksum="8FC3B5F344EC7FB8" modified="2017-01-18" version="1">MGLSSNWKSLKTSNEVNSKTVDKQQGANKNFNSKKNSVGKKFTPNKTTAITKPSSVKKTGRIKSVVDNITKQIQAQSAKRNQEKEVGSIVSVNLQKIIDEDNKTRSLSKNVGEIGKYLAIDCEFVGVGPEGTESVLARVSIVNFYGHTVYDEFVKTKERVVDYRTWVSGIRPGDLLNAEKFEDVQQKVADIIKGRILVGHSVYHDLDALLLSHPKSMIRDTSRHVPYRQKYSNGKTPSLKKLAKEVLNVEVQDAEHSSVEDAKVTMLLYKFDKKEFERLANTKFYNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R193</accession>
    <name>A0A1E5R193_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Poly(A) ribonuclease POP2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4312</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R193">
        <property type="interactions" value="997"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R193"/>
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    <dbReference type="OrthoDB" id="1164111at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039637">
        <property type="entry name" value="CNOT7/CNOT8/Pop2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10797">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="127"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="494" mass="55192" checksum="59080078240A0FDD" modified="2017-01-18" version="1">MDLLRLQQQQQQHQAQQLQQHQGGRVHPNMMPFHENPSSHIMSPPGQHILNPKNLPTNNSAGGAKPENEFLLALQKGKSQQSQPQMQHNNQQPPGLGPENGNSSSFHPXLPPPGLHLQGRPSLGQTNFQNNINGGANNPMMTQNMQPSQYTNESNASGAYPEKVDGLNGPLPHQMGPHGPPIPHPLGMPQIPLELQPQNIIKHMVKDVVSSNLYKEFNAIRQLSERYNLISVNVEFAGTLARPIGRFRGKSDYIYQTVRTNVDLLDVLKIGISLSDEFGGKPDMSISTWQFHFKMSPQTQMIPIETIELLQKTSTCADTLPQNLVTKGVDFKEFATLLTESGLILNPNVMWITYSAGYDLAFLTQMLKNTFMPNNKEDYIEICKQYFPNFYDLDLINQHLKAAIIQMNXQQAGLNISTNLEDTKVASQTIEVLAEELQIPHFPHHNTAGGQSLLILTIFHKISKITNRRFPDGRDFGILNGEMFGVSTEKESSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1E3</accession>
    <name>A0A1E5R1E3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Clathrin heavy chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g4321</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasmic vesicle membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <location evidence="6">Coated pit</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the clathrin heavy chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1E3">
        <property type="interactions" value="1286"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1E3"/>
    <dbReference type="InParanoid" id="A0A1E5R1E3"/>
    <dbReference type="OrthoDB" id="2113814at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030132">
        <property type="term" value="C:clathrin coat of coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030130">
        <property type="term" value="C:clathrin coat of trans-Golgi network vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071439">
        <property type="term" value="C:clathrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032051">
        <property type="term" value="F:clathrin light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000001">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000002">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000082">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.110:FF:000003">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.110">
        <property type="entry name" value="Clathrin heavy-chain terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055358">
        <property type="entry name" value="CHCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015348">
        <property type="entry name" value="Clathrin_H-chain_linker_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016025">
        <property type="entry name" value="Clathrin_H-chain_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016341">
        <property type="entry name" value="Clathrin_heavy_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10292:SF1">
        <property type="entry name" value="CLATHRIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10292">
        <property type="entry name" value="CLATHRIN HEAVY CHAIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00637">
        <property type="entry name" value="Clathrin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09268">
        <property type="entry name" value="Clathrin-link"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13838">
        <property type="entry name" value="Clathrin_H_link"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002290">
        <property type="entry name" value="Clathrin_H_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00299">
        <property type="entry name" value="CLH"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50989">
        <property type="entry name" value="Clathrin heavy-chain terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0168">Coated pit</keyword>
    <keyword evidence="5 6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Clathrin heavy chain linker core motif" evidence="8">
        <location>
            <begin position="344"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="548"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="702"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="851"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="997"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="1146"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="1292"/>
            <end position="1438"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="1441"/>
            <end position="1584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF09268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1658" mass="189564" checksum="F1CE5C12CF164F25" modified="2017-01-18" version="1">MSDLPIDFTELADLQSLGISSNFFDFKSTTFESDKYVLCKESLQDGTQNTVSIVDLSNNNEIVKKNMGGDNAIMHPSEMIISIRANGIIVQIFNLNTKEKLKSFQMDEPIVFWKWLNNEKIGIITTNSIHVLNVFDNNPMGKPTRMTARHETLNNAQIIDFNCNENADWFALVGISQNTENKIVGKIQLYSSKRNISQAIDGHVAQFAKIHLDGNPTTNFNQVFITANRSYTEGNNLLKIIEIDHDASLTPTSFVKKQQDIFFPPDATNDFPLSVKVSEKYGVIYLLTKFGFIHLYELETCTNLFVNRISGDSVFTTTNYNNNSGIACINKRGQVLAVEISTSQIVSYILEKLGNVELALKIASRGNLPGAESLFTKQFDQLIATGDYTQAAKVAASSNILRTPETINKLKNLPVSNANAISPILIYFSTLLENNKLNKFETLELTKPLLQQDRKNLFEKWLKEDKLECSEELGDIVKPFDLTLSLACYLRSQSHLKVLNCLVELQQFDKIIPYCEKYQFKPNFTSLISQLILTSPDKASEFAVSLLNNSPPIELDLETVADLFFKNNQIQQGTSFLLDALKQDLPEFGHLETKVLEVNLLHAPQVADAILNNQMFHHYDKHTIASLSEKAGLYQRALENYSDLKDYKRCIVHVNSLPMDWILGFFGTLNVNDSLSLLRVMMESAQQPTTGNNTLQIIIQIATKFSELFGSATLIKFFEEYKSDEGLYYYLSSIVNLTDDKDVVYKYIQAAAHLKNYKEVERIVRDNNVYDGEKVKNFLKDSSLEDQLPLIIVCDKFNYIHELVLYLYKQQNFKFIEIYVQQVNPSKTPQVVAALLDVDCDEKFIQSLLSSVVSTGVVPVNQLCDEVEKRNKLKILLPFCESLLHSNVQDVAIYNTLAKIYIDSNNSPETFLKENDQYDPVNVGKYCEKRDPYLAYIAYEKGGCDEDLIRITNENQMFKYQARYLLHKSDSQLWNSVLSSENEFRKSLIDSVISVGIPELTDPEPISIVVHSFMANDLKTELIELLEKIILEASPFSENSALQSLLMLSAIRYEPAKVVNYIDKLDKYDPAEIAPMCVENGLLEEAFKIYDQNEMFDKAMKVLVEDIMSLDRGYDYAEKIDQKALWSQLGEAQLNGLRITEAIQSYIKAEDPSNFEHVIDIAEHSDKLEELVDYLKMARKSLKETKIDGTMILCYSELNKLNEVETFLQNSNVADLEEVGDKLYEKKNYKAAKLCYQSVSNYSKLATTLVRLDDFQQAVDTARKASNIKVWKQVSDACIDEKEFRLAQVCGLNLIVHAEELNDLVEKYESLGYFEELISLFEQGLGLERAHMGMFTELAILYTKYNPAKTFEHLKLFWSRINIPKVIRAVEATHLWPELIFLYAHYDEWDNAALTVIEKSTDNFDHLYFKEIIVKVSNLEIYYKAINFYVKEHPSLLVDLLTVLIPRLDIPRTIRLFQKSDNLPLIKPFLINVLPKNISIVNQAYHELLVEEGDYVSLRDAVESYDKFDQLELAKQLEDHELIFFKKIAASLYSRNKKWIRSLAILKEEKLWRDAIQTAVMSQSSEIVEELLSYFIDTGNREAFVALLYTGYHLVRYDYVLEEAWLNGLTDFIKPYEISVKKEQFDAVKKLQEKLSSTQISDENDGESPLLLANGSAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1J5</accession>
    <name>A0A1E5R1J5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Chromatin modification-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AWRI3579_g4056</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Component of an histone acetyltransferase complex.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair. Involved in cell cycle progression and meiosis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of an histone acetyltransferase complex. Interacts with H3K4me3 and to a lesser extent with H3K4me2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="16">The PHD-type zinc finger mediates the binding to H3K4me3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the ING family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1J5">
        <property type="interactions" value="310"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1J5"/>
    <dbReference type="InParanoid" id="A0A1E5R1J5"/>
    <dbReference type="OrthoDB" id="3971228at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16858">
        <property type="entry name" value="ING_ING3_Yng2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15505">
        <property type="entry name" value="PHD_ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000436">
        <property type="entry name" value="Chromatin modification-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028651">
        <property type="entry name" value="ING_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024610">
        <property type="entry name" value="ING_N_histone-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333:SF100">
        <property type="entry name" value="CHROMATIN MODIFICATION-RELATED PROTEIN YNG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12998">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01408">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7 16" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0469">Meiosis</keyword>
    <keyword evidence="3 14 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 15 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="18">
        <location>
            <begin position="277"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="202"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="13">
        <location>
            <position position="279"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="13">
        <location>
            <position position="290"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="13">
        <location>
            <position position="294"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="13">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU361213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OEJ80778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="332" mass="37261" checksum="93CCDACEABF1C3FA" modified="2017-01-18" version="1">MTKQECAIGATRLQPSTXKIKADSKRLYSELQMNVTDPSSLLETTLSSISNLQKETQFVLSEIRDSDQEFDTLRKFIETQDTLIQKHIIKNGAIQENPKENEIKAAIQEAFGKCKKLQERKNALANTLLYITTMKCKDLQNGMDILEADGVLAPNEIVEVEAPDSIKNILKQKYHQQLEDYESDSLYNSEYDDSDVSIATTKKRKANKAQKIANKNGSAGLKKSKQQAASVNKKHTKSASTEPHDLLADHTGTTAAGATNPGFSKTQNDEDEDDDKQLYCFCQNVSYGEMVACDGNDCKYEWFHYSCVNLTEPPKGKWYCPDCKSRMSKNLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1M2</accession>
    <name>A0A1E5R1M2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Suppressor of stem-loop protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g3999</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GTF2H2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1M2"/>
    <dbReference type="FunCoup" id="A0A1E5R1M2">
        <property type="interactions" value="1038"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1M2"/>
    <dbReference type="InParanoid" id="A0A1E5R1M2"/>
    <dbReference type="OrthoDB" id="284275at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01453">
        <property type="entry name" value="vWA_transcription_factor_IIH_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000477">
        <property type="entry name" value="General transcription and DNA repair factor IIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000015">
        <property type="entry name" value="General transcription factor IIH subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007198">
        <property type="entry name" value="Ssl1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004595">
        <property type="entry name" value="TFIIH_C1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012170">
        <property type="entry name" value="TFIIH_SSL1/p44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00622">
        <property type="entry name" value="ssl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12695">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12695:SF2">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07975">
        <property type="entry name" value="C1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04056">
        <property type="entry name" value="Ssl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01047">
        <property type="entry name" value="C1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="VWFA" evidence="13">
        <location>
            <begin position="207"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="56"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="144"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ80796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="543" mass="61555" checksum="E486784D2E3B0202" modified="2017-01-18" version="1">MFSDEEGDRDESIVVNRRKRPGLGTEEDERSATPEGVANNNLQLKKRKTVAFSNEEGGYEDDDLSGNFPAAKQNKDRLKNFRDQISSNKRRLKKRVEESESDADEEVPGKPTSKQKARHKSSDRERRREKGKNKKGSSSSGSSNKKESKESENNGYAWEDKIQRPWDIVDAAVGDDDETIIAAKIMELRRKRASIDIKPFQRGIIRSLIIAIDFSEVMGEKDLRPSRLHMSVQYCIEFVYDFFDQNPISQLGVISMKNGVAQVVSPMSGNPQDHIEILQNLKRKLEPKGFPSLQNGLELSRGLLMNAPAHSTREILIIYGSLSSTDPGDIHQTIQSLVEEKIRVNIIGLTAQVSICKELCKQTNYGDESFYNVILNEQHFKDLVMEAVTPLPMNKLNKSFTLVKMGFPTRAVEDTPSFCACHSKLVHGGYYCPNCNNKVCSLPIVCPCCQLMLILSTHIARSYHHLIPLKTFKEVPIQNDFPTNHCFGCQRRFPKLENKKTGELLTSMRYRCDDCKHDFCIECDAFVHEMLHNCPGCESKTNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1Q7</accession>
    <name>A0A1E5R1Q7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ER-localized J domain-containing protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4354</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DnaJ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1Q7">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1Q7"/>
    <dbReference type="InParanoid" id="A0A1E5R1Q7"/>
    <dbReference type="OrthoDB" id="413400at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000116">
        <property type="entry name" value="Erj5p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052606">
        <property type="entry name" value="DnaJ_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44653">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44653:SF2">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184590" evidence="11">
        <location>
            <begin position="21"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="12">
        <location>
            <begin position="44"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="245"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="13">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ80830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="270" mass="31694" checksum="86136A46C5D13D0B" modified="2017-01-18" version="1">MFSXKSILFSLCLLIEXCLCFTTDEVEIFELQKDIVKKYGKSIDFYKLLKLNKGAQSTDKEITKQFRALSRKYHPDKNVKYTKLYQRLNNAKKILLNSDARKSYDYYLKNGFPKYNFARGGYYFERYSLKGAQVMFVVFLFLNVIHYFFMKIQVKSKRANIDNFINQIKEQYDDTDGMGIKDLQFDTSEAKDGSQVDRMKIKFGQVYTVEETGXEILMDSHQLVPDTQFRDVFICKLXQKILPKKSSAEKQPQKMNKKKTKGLAKTTKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1W8</accession>
    <name>A0A1E5R1W8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MEMO1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3881</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MEMO1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1W8">
        <property type="interactions" value="508"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1W8"/>
    <dbReference type="InParanoid" id="A0A1E5R1W8"/>
    <dbReference type="OrthoDB" id="417112at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07361">
        <property type="entry name" value="MEMO_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.830.10">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00055">
        <property type="entry name" value="MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002737">
        <property type="entry name" value="MEMO1_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04336">
        <property type="entry name" value="AmmeMemoSam_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11060">
        <property type="entry name" value="PROTEIN MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11060:SF0">
        <property type="entry name" value="PROTEIN MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01875">
        <property type="entry name" value="Memo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="323" mass="36735" checksum="7F4B22EAC5B75735" modified="2017-01-18" version="1">MDSIRPATHAGSWYSSNKKALMSSMNQWIKTPLQTGNKARIVVSPHAGYTYCGKVLGQAYSKLNIDENTETIYVLGPSHHVYFQNKVLLSSFDFVETPLGNIPVDRSKIHAWKTKYPHFFEEMDEDTECEEHSLEMQFSPVKFLLEAHKNTKAKIVPMLISHNTEKIDYELGDILGKEMGKSKNTVFVVSSDFCHWGXRFRYTAYVANESDLWHHFESNIELGNLNKKNLDALETPIYKSIEIMDKGAMKVLEEHSGKDAYKNFKKYIKVTGDTICGEKPLCVVLAALRKQKTVFHWSGYEQSSRCTSVTDSSVSYGAGYCKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R269</accession>
    <name>A0A1E5R269_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Mitochondrial fusion and transport protein UGO1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g4060</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R269"/>
    <dbReference type="FunCoup" id="A0A1E5R269">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R269"/>
    <dbReference type="InParanoid" id="A0A1E5R269"/>
    <dbReference type="OrthoDB" id="77989at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="163"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="207"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ80996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="610" mass="68395" checksum="3B0520ADE0147B90" modified="2017-01-18" version="1">MSADTNGTGTSNALRPYYVADTFDCGYVAKYKPFQGIVDGNGINLASKLNIFSMGKSRSGSRDFSRLSLNDLGSSAGKNSTRSAIALGGTQNGNSPLSGMGRWSTGNRDKSFREMELADYFNWRIWENFFRQIMKGVLKEYVKHLIQQPFEVAKVVMQVRAEDTDSKSVNHYTPSASRGSSNDTTLGKSILSPGVENTLQDVHSTIHPPATPSISTHWNDNHAQDNSDSEEEINYFQSKEAAANIAADSYSSQSAALGQGQSDHFSTSFHGNCIQPQTNTTLDILSSILDKEGIKGIFRSLHTSFILQFMTNTVDAWFTGFLAPLLNIPDPYFVDLIHSNNIMRSVVLSLCASVTTGLVLLPLELINTRFIITTTATKTTVQKSQPTELKEPIGADSPVTGSPAHEIWDNAEDSETQVQPRSMRKLLSAWKWKNNIKSLNFNILSMNILNSCFTMIFNKQLIHTFLVYYCQLDKFGGQSSSFIIYHTAKFMIKVLQLFIQLPVETIYKNCQVDYLLYTRDANPFYIEDKSALIIKPLVWRDGHTPNSQQDADGEQSLSKIKQNNAYVWYKLKGNFACLWRGWKLGMMSVLCGYGLKLMKYQDNETREVRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R281</accession>
    <name>A0A1E5R281_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA repair protein RAD34</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R281">
        <property type="interactions" value="83"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R281"/>
    <dbReference type="InParanoid" id="A0A1E5R281"/>
    <dbReference type="OrthoDB" id="300780at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000111">
        <property type="term" value="C:nucleotide-excision repair factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071942">
        <property type="term" value="C:XPC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.260.10">
        <property type="entry name" value="Transglutaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004583">
        <property type="entry name" value="DNA_repair_Rad4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036985">
        <property type="entry name" value="Transglutaminase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12135:SF2">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12135">
        <property type="entry name" value="DNA REPAIR PROTEIN XP-C / RAD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="2">
        <location>
            <position position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="690" mass="81726" checksum="0E50B3AABE145AA9" modified="2017-01-18" version="1">MSGGENDTHPSDFYEPVMXENNEKDNXLWSQSSNAIEQNDGFDEDDSENEDNFEWEDVPLDSFKFEVKHTKTKKQVSIEKRKRSLDFKHKWLNNAFFCHIQEIPLFLSIFQKQCITWVGDYRNYTLIKKKVIPKLIKKKFSDWHKLPRGSSIKDNKCRTLLLGACMWFRSTYKINGNGVRQTPYRLRNLIFNKQYANLAAKTKKATLAETNTEMYYGRKSPTASDFRLAIKTKKANRDQLVFVFYIILLALLDLSDKDDELRIVYSLPVHDYKRVQCLAMQSVETIXRLDHKCPNISDSDLFSPYYWIEYKTNNYIYTIDPVVELDRDSIVQRVKINEYQPHFQKNCSKSGLDIQTHYYVVGVNEKFEIMDLSPRYLNNIMYRVEPCLINIDKNKDWXSFCVYKNALKKKGNYACNFVDVWDSQELQDLQRMSLQNMEMPKTLYALKTNPNFVGDLFVRKNLEIIKGKPVNFGNTHKNSITVYLKKDLHRLRSEYHWLILGRSLNDKAQASYSRSTYKKSLNCMNNGVKKRKLYTQTNELEVVDLYTFDQTSPTPRLPNLPSIDHYKNQFGDIKIFNIQQIKPTEFEVVRKLTTYPNSFVVDNVKLIRKHNSRQSDFNKKLQYIEVVTGFDFXKRPGYAVPLKNHIMLSEXHHQQLLVLQQREIELHYLNEWYWLLKKLQVQKRVEQSFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2C4</accession>
    <name>A0A1E5R2C4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CDC25-like phosphatase YCH1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3874</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2C4"/>
    <dbReference type="FunCoup" id="A0A1E5R2C4">
        <property type="interactions" value="194"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2C4"/>
    <dbReference type="InParanoid" id="A0A1E5R2C4"/>
    <dbReference type="OrthoDB" id="102559at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828:SF38">
        <property type="entry name" value="ARSENICAL-RESISTANCE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE DUAL SPECIFICITY PHOSPHATASE CDC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="25"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="151" mass="17045" checksum="3D844CAA4A6FC231" modified="2017-01-18" version="1">MQRSINSVKYLDASELHKMLLNKKPAAPFKVVDVRGSDFLGGHIKGCLHFPYSQLKHDDEYLQELKQALLSNTQPESASTTVPIKCIFHCAMSQQRGPSAALLFLRSLTEDEFNSGIFEVYILKGGFIHWVSAGYGTDAKVTEDYLDYLWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2C6</accession>
    <name>A0A1E5R2C6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">V-type proton ATPase subunit D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4193</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase D subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2C6"/>
    <dbReference type="FunCoup" id="A0A1E5R2C6">
        <property type="interactions" value="877"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2C6"/>
    <dbReference type="InParanoid" id="A0A1E5R2C6"/>
    <dbReference type="OrthoDB" id="7676488at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002699">
        <property type="entry name" value="V_ATPase_D"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00309">
        <property type="entry name" value="V_ATPase_subD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11671">
        <property type="entry name" value="V-TYPE ATP SYNTHASE SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01813">
        <property type="entry name" value="ATP-synt_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="236"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="236"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="252"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="268"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ81038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="282" mass="31712" checksum="47293F8809FE7EC3" modified="2017-01-18" version="1">MSATREQVFPTRMTLGLMKTKLKGANQGHSLLKRKSEALTKRFRDITKRIDDAKTTMGRVMQTAAFSLAEVQYATGNDNLSFQVQENVSSKARFQIKAKQDNVSGVILPHLEAFIDENINDFKLTGLGKGGIQVSKCIKIYTKAVETLVELASLQTAFIILDEVIKVTNRRVNAIEHVIIPRTENTIAYINSELDELDREEFYRLKKVQEKKQIKMKELDDQLKEQLKLDTVSKAEGAAAASVVSEVDQKTKSKNGKSKKSGKKETKEDEDLVAENEDDVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2F2</accession>
    <name>A0A1E5R2F2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">GPI-anchor transamidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4102</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2F2"/>
    <dbReference type="FunCoup" id="A0A1E5R2F2">
        <property type="interactions" value="669"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2F2"/>
    <dbReference type="InParanoid" id="A0A1E5R2F2"/>
    <dbReference type="OrthoDB" id="192611at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003923">
        <property type="term" value="F:GPI-anchor transamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1460:FF:000003">
        <property type="entry name" value="GPI-anchor transamidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028361">
        <property type="entry name" value="GPI_transamidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001096">
        <property type="entry name" value="Peptidase_C13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48067">
        <property type="entry name" value="GPI-ANCHOR TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48067:SF1">
        <property type="entry name" value="GPI-ANCHOR TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01650">
        <property type="entry name" value="Peptidase_C13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500138">
        <property type="entry name" value="GPI8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019663">
        <property type="entry name" value="Legumain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00776">
        <property type="entry name" value="HEMOGLOBNASE"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5028005980" evidence="7">
        <location>
            <begin position="21"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="361"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR019663-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="384" mass="44421" checksum="9F74A87CC0D9C5AD" modified="2017-01-18" version="1">MQLLNQLFIVFSVLVLFVSSENNEHTNNWAVLVCTSRFWFNYRHMANTLSMYRTVKRLGIPDSQIILMLSDDVSCNSRNLYPGAVFNNMDHSLDLYGESVEVDYRGYEVTVNNFIRLLTDRWDEDQPKNKRLLTDENSNIFIYMTGHGGEDFLKFQDAEEIASQDIADAFQQMHEKKRYNEIFFMIDTCQANTMYSKFYSPNILAMGSSELDESSYSHHSDVEIGIAIIDRFTFYALEYLEKIKEKNSNLSIQDLFDYLTFERVHSHAGMRTDLYGKNVSQALITDFFGSVQEFLPDTAVVDLENSKTVLDLAVEQYNEAPNPEEYLAKTKFVNAKSAINRNMQVGQDQLLLSDEQSIKKYFVAFFILLSTLFFSTLVKRVPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2I6</accession>
    <name>A0A1E5R2I6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Homoserine kinase</fullName>
            <ecNumber evidence="3">2.7.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g3905</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Commits homoserine to the threonine biosynthesis pathway by catalyzing its O-phosphorylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-homoserine + ATP = O-phospho-L-homoserine + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13985"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.39"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:13986"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GHMP kinase family. Homoserine kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.39" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2I6"/>
    <dbReference type="FunCoup" id="A0A1E5R2I6">
        <property type="interactions" value="191"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2I6"/>
    <dbReference type="InParanoid" id="A0A1E5R2I6"/>
    <dbReference type="OrthoDB" id="195231at2759"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00064"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004413">
        <property type="term" value="F:homoserine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000068">
        <property type="entry name" value="Homoserine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.890:FF:000016">
        <property type="entry name" value="Homoserine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00384">
        <property type="entry name" value="Homoser_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000870">
        <property type="entry name" value="Homoserine_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00191">
        <property type="entry name" value="thrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF1">
        <property type="entry name" value="HOMOSERINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861">
        <property type="entry name" value="HOMOSERINE/4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000676">
        <property type="entry name" value="Homoser_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00958">
        <property type="entry name" value="HOMSERKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0791">Threonine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="13">
        <location>
            <begin position="73"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="14">
        <location>
            <begin position="272"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ81094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="357" mass="38845" checksum="0308E3FBCF106296" modified="2017-01-18" version="1">MPRSFEIKVPASSANIGPGYDVLGIGLSLYLSLKVTIDPKTADQSNKDPNNCLIEYTADSEGYDSVPLQSDKNLITKTALYVLRCNDIRQFPTGTHILIDNPIPLGRGLGSSGAAVVAGVMLGNEVGQLGFSKQRMLDFCLMIERHPDNITAAMMGGFVGSFLRDLTAQEMERREIPLSEVLDAPSGGEDTGLSPPLPPTDIGRHIKYNWNNKIKCIAIIPDFEVSTADSRGVLPSAYTSKDLVFNLQRLAVLTTALTQDPPNPDLIYPSMQDRVHQPYRKTLIPGLTEILKSVTPKTHSGLLGICLSGAGPTILAVATDNFEDIAQEIINMFAKKNITCRWKLLDLAYEGATVERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2V0</accession>
    <name>A0A1E5R2V0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Brl1/Brr6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4377</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2V0">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R2V0"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006998">
        <property type="term" value="P:nuclear envelope organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040202">
        <property type="entry name" value="Brl1/Brr6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018767">
        <property type="entry name" value="Brl1/Brr6_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28136:SF1">
        <property type="entry name" value="NUCLEUS EXPORT PROTEIN BRL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28136">
        <property type="entry name" value="NUCLEUS EXPORT PROTEIN BRR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10104">
        <property type="entry name" value="Brr6_like_C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01042">
        <property type="entry name" value="Brr6_like_C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Brl1/Brr6" evidence="2">
        <location>
            <begin position="8"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="197" mass="22955" checksum="94C2AB3C83A364DE" modified="2017-01-18" version="1">MQITHHKIFIATIYVVFSYLLIQFIKTFYHDVSLLFQQEHLKNLSLLQNCQLQYKQNYCDPKKRVPELYELCHELSVCIDRYXKLSNAQLTLKTELMVKHLMSLIEIASXRLSYKTLLVMLLAVLVIIALQQTLILKAYRDTMPARSFNDXAXSVNQMRSCNNTDSTKTSMYQSFENXEKNNVNGSYESILXVEEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2V9</accession>
    <name>A0A1E5R2V9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">4-hydroxybenzoate polyprenyltransferase, mitochondrial</fullName>
            <shortName evidence="10">4-HB polyprenyltransferase</shortName>
            <ecNumber evidence="10">2.5.1.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">4-hydroxybenzoate hexaprenyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Para-hydroxybenzoate--polyprenyltransferase</fullName>
            <shortName evidence="10">PHB:PPT</shortName>
            <shortName evidence="10">PHB:polyprenyltransferase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">COQ2</name>
        <name evidence="11" type="ORF">AWRI3579_g4291</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Catalyzes the prenylation of para-hydroxybenzoate (PHB) with an all-trans polyprenyl group. Mediates the second step in the final reaction sequence of coenzyme Q (CoQ) biosynthesis, which is the condensation of the polyisoprenoid side chain with PHB, generating the first membrane-bound Q intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>an all-trans-polyprenyl diphosphate + 4-hydroxybenzoate = a 4-hydroxy-3-(all-trans-polyprenyl)benzoate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:44504"/>
            <dbReference type="Rhea" id="RHEA-COMP:9514"/>
            <dbReference type="Rhea" id="RHEA-COMP:9564"/>
            <dbReference type="ChEBI" id="CHEBI:17879"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58914"/>
            <dbReference type="ChEBI" id="CHEBI:78396"/>
            <dbReference type="EC" id="2.5.1.39"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Mitochondrion inner membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
            <orientation evidence="2 10">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the UbiA prenyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.39" evidence="10"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2V9">
        <property type="interactions" value="349"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2V9"/>
    <dbReference type="InParanoid" id="A0A1E5R2V9"/>
    <dbReference type="OrthoDB" id="18170at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008412">
        <property type="term" value="F:4-hydroxybenzoate polyprenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13959">
        <property type="entry name" value="PT_UbiA_COQ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1780:FF:000001">
        <property type="entry name" value="4-hydroxybenzoate octaprenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.140:FF:000003">
        <property type="entry name" value="4-hydroxybenzoate polyprenyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1780">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01635">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006370">
        <property type="entry name" value="HB_polyprenyltransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039653">
        <property type="entry name" value="Prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030470">
        <property type="entry name" value="UbiA_prenylTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01474">
        <property type="entry name" value="ubiA_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048:SF28">
        <property type="entry name" value="4-HYDROXYBENZOATE POLYPRENYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048">
        <property type="entry name" value="PRENYLTRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00943">
        <property type="entry name" value="UBIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="166"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="269"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="352"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="393"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00052313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ80913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="414" mass="46386" checksum="A4AD0A30435FE010" modified="2017-01-18" version="1">MVKMFLKPLNLRYLSWNARPXSFSLNKTANXLLNTGSTKSLFSLIEITTKPQNLAFGXRRYQSTLSNXNTKTKAQEKWDEIAKTPEHKYQELIQSNVFTNEQIKESEKARMVSLGRLKKLPKAIIPYAELMRLDKPVGTWLLYIPCTWSILIAAIMQSSALGLTXYMLSVFGIGSLIMRGAGCTINDMCDKNLDDKVIRSIQRPIASGRVSTPQATAFLAAQTAIGVGILATLPAQCWWFGLVSLPIVFAYPLFKRFTYYPQAALSSCFNWGALLGFPAMGLMPWSVILPLYGSTFIWCMIYDTIYAHQDKKFDINAGIKSTALKWGDKTKSIFNGLSAAQMGLMTLSCYNAGILMGPGVLVGLGIFGYRLLQMIRKVDLNNPADCWKWFQSNIKSGLLFTVALILDYLWVLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R316</accession>
    <name>A0A1E5R316_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">U3 small nucleolar ribonucleoprotein protein MPP10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g4035</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
            <location evidence="1 7">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the MPP10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R316">
        <property type="interactions" value="1005"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R316"/>
    <dbReference type="InParanoid" id="A0A1E5R316"/>
    <dbReference type="OrthoDB" id="445326at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034457">
        <property type="term" value="C:Mpp10 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005732">
        <property type="term" value="C:sno(s)RNA-containing ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012173">
        <property type="entry name" value="Mpp10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17039">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN MPP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17039:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN MPP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04006">
        <property type="entry name" value="Mpp10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017300">
        <property type="entry name" value="snoRNP_Mpp10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3 7" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="93"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="262"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="545"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="611"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="97"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="163"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="288"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="305"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="551"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="562"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="623"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR017300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="635" mass="72541" checksum="269E59E776E46791" modified="2017-01-18" version="1">MSANSISQLLSNNPIGILNDAFVESTEPLKLVKSKLDELIKIQQSQFESKTALDEVAVDGLDANQVWWQAKIVLDKIEPNLMDHILELKDVLRQQESEDSSEADSYSENESEAGLEDKDEQEEQERNYESSDGEDVSEYMTAENIEPQNELVSEEVNEDEIQATGSDNENFQSNLEEEEEEEEEEEEEEEEEEDDRDPFFDAEAFNKQMDIADEKGKDILGSANNNDEEQEEXIDYFGDLPSEDDEEVMYYNDFFDKPKNIKKTTNFKRDKSKTGTSGAEDLDEEQDFSDKEHQEAMEAAKLDLFAEEQSDDEEDADQQPHKAAGKQLSTFERQQLEIQRQIEQLEKEAVADKKWAMKGEVKARDRPDDALITEDLEFDRASKPIPVITAEVTESLEEMIRRRVKESDFDDLPRRLITDLPKMRSRAQFELSDTKSSKSLAEVYEEDYQSSKTVGGEENEVSEELQKSHDEISEMFSNLMYKLDALSSAHFVPKPQTKALEVKVQSAAIAMEDAQPLTMSSASTLAPQEIYKVGKSENSEEITLKNGTVMAKEELSREDKQRLRRSMKRKRAKAFSNREHKTAEQQNQKKAKHQDVIETLSAAKNITVIDKKGQKSDVRGNVQKASSRSTNDFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4I6</accession>
    <name>A0A1E5R4I6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">General control protein GCN4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3699</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the bZIP family. GCN4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4I6">
        <property type="interactions" value="3664"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4I6"/>
    <dbReference type="InParanoid" id="A0A1E5R4I6"/>
    <dbReference type="OrthoDB" id="3972958at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001080">
        <property type="term" value="P:nitrogen catabolite activation of transcription from RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903833">
        <property type="term" value="P:positive regulation of cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12193">
        <property type="entry name" value="bZIP_GCN4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001491">
        <property type="entry name" value="Cross-pathway control protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050946">
        <property type="entry name" value="AP-1_TF_bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11462">
        <property type="entry name" value="JUN TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11462:SF35">
        <property type="entry name" value="TRANSCRIPTION FACTOR JRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="11">
        <location>
            <begin position="268"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="183"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="280"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="192"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="12">
        <location>
            <position position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ81827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="316" mass="36194" checksum="84514947C55FFB47" modified="2017-01-18" version="1">MSLFTTTTNVALSEAQFFAHSQQQQQQEXHEXEQEQEQEQESFDFNNFMLNNDSESELDSAKTSNSFENVNPASFIADWLKEDDPIFTFEEDETMATLKYEKDQLQQQKDEEXKENAVSHEQDNYWNCLFDDIEKPMMDTIADSSFVKQEKNEEEEQTDLLNLNNTTVSFGFLPTPVLEEDEKLSVKETKSKPXTAQKIATTSSKPNAKANKVTKTKKSTSPFTPASNSASTSPKLDELGIVSYTTKKRSMPLNEIKMDDLQDPAELKRAKNTEAARRSRARKFQRMQQLEDKVAELLEINANLVQEIERLKNGNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4Z5</accession>
    <name>A0A1E5R4Z5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">26S proteasome regulatory subunit RPN3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3539</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4Z5">
        <property type="interactions" value="1202"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4Z5"/>
    <dbReference type="InParanoid" id="A0A1E5R4Z5"/>
    <dbReference type="OrthoDB" id="1713558at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050756">
        <property type="entry name" value="CSN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013586">
        <property type="entry name" value="PSMD3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057985">
        <property type="entry name" value="TPR_PSMD3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758:SF2">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3/COP9 SIGNALOSOME COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08375">
        <property type="entry name" value="Rpn3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25573">
        <property type="entry name" value="TPR_PSMD3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="265"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ81970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="519" mass="59608" checksum="613B7261F2532653" modified="2017-01-18" version="1">MSDPMEVDNLNHNESAMLNGKATTVKDTFTSAQPVDEMIDLCKEIVKSTTLVDQRYIYKALKKLPVLRTKLTKENLTVFVDLLYPASSAFKSSLMKVLGNTQKSHVEDEETIRNGYPIEFYELTXEENVEVTAELVTFTHLLVHLYLFKTNELQKSSKFAISETIPKVLNTFNSRILDLINAKLWFYVARCNELLPISEKQESIRFEMIKFLKVTALKHDVETQAMLICCILREFIQAGEISSASDFISKVDFPSNEGISSSLEARYFYYLSKINSIQLDYSTANEYIIAAIRKAPNSSSSLGFLQSAFKLRCCIELLMGDIPELSFFKQDKMEKSLYPYYLLSKAVKTGDLNKFQETIHKYKQPLVKDDLYQLCLRLRSNVIKTGIRIVSLTYKRIALKDICLKLRLDSEQSAEYIVSKAIRDGVIEAKINYEKGQVETSELLNIYDTEEPQSIFNERIKFVTELHDECVKAMRFPEKRKSNKDTGKDDGSKEVSVEVLEQLLSDLSDFEDDEDDFDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R531</accession>
    <name>A0A1E5R531_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CAP-Gly domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3673</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R531">
        <property type="interactions" value="652"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R531"/>
    <dbReference type="InParanoid" id="A0A1E5R531"/>
    <dbReference type="OrthoDB" id="5273213at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CAP-Gly" evidence="2">
        <location>
            <begin position="21"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="556" mass="64886" checksum="F5C691CF812840EE" modified="2017-01-18" version="1">MKNNLAAMAKNGPGYCXXLNIGDRLEIESSFCTVRYIGVLFXQEQQRGEKSEQTQGEQENVIIIGIEWDNDHCGKTKNGILKGTQYFEPRRKNCLNSSFYRLNSSFRNKYISNVRKSFSRYITDLFESDYHNQAPFLSSLNQLSLDSQKSAELVGFDEEKRKKMLLKSESLDLSYCKIWTYLDEEHENFKIEKYQKYFDRVTALNISYNLISKAETVHKIIAWFPNXQTLIMNGLRFSSYKLDYPLFSPSLRFLNLSNHQTPDVLQLLPDNQEFEVLDLSKNNLGALEQTPIAIKKLDLSSLHSVELNLSFNDLQLVPHISETVRHLNISNNQISQNMNEESQKRAYLNMLSLNIGSNKISHWDGLHELVINIFPQLXSLNFNKNVIQTKNLXESSDDXYENVIARLPHNITCLDYNSIVLXEQKDCQRYLINKWKRGEIPHADLLLXRLLEXNNISVTYXNAISDVQNSSSFLXLDVLPYNFKIRTLKTCTVRYLKFQIFERLGKPAKYYDYYKNMTXKYRIVNEVYETFKYDFTPLSDYNIQTNDKIFVSFVQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R555</accession>
    <name>A0A1E5R555_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Alpha-1,3-mannosyltransferase MNT3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3667</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MNN1/MNT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R555">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R555"/>
    <dbReference type="InParanoid" id="A0A1E5R555"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000033">
        <property type="term" value="F:alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392:SF1">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="11">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ82045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="586" mass="67522" checksum="6022FD4C6A16C029" modified="2017-01-18" version="1">MVSTEIQNLEQEAQRGSHLDGXTSTTQANGEKQSHLEKCSKYFHKLYRKNPEWNYEDIKYFFNDDTVDDKLLAEMMETARVYFDCYFGTEKDDYFKNAFPDLESRIFPFLHPNFTTGLTAFTPTVTKYSFQPDEQDLESSILVEELPFKEASGTLSLLSSSEYSRGDGVVLTMGQNHLDILPRFLNVLSYLQTNTPVQIVLKDLELEFPEPEKRARLLEILHKYNVEKGLKVEILDCSKLLNPEFIQSXIINVLNKWIAMIFNSFENFLLLDIDVVIFEKPEFFFQLPDFESEKTSMIVFKDRHLVEERTFSYCIDMFKKLLPKXFDRVSLKKENPELNYIFEKFFDDRMLHSVDSGLVLVKKLKALPQLLTAMQLNVAGKPIRCVYGDKEFTWLSYLFLGDTTPESIKVVPFYGGIAGRASEPQKSGQETLIEVCGTQLAHVNPFTNKLLWVNGGLKTCKMGREAAEKDFSEHYDYFNEKFGQPDAKNSKXKTLTLKQLENIYDNPLRIDTVVIPDTTSPLKKWYQSGECSEYFYCAVSNTTSSVGLDDEVNLGEGFYVSLTENQDQLQHVNEISLQWNRGASDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R798</accession>
    <name>A0A1E5R798_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cofilin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Actin-depolymerizing factor 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3488</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the actin-binding proteins ADF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R798"/>
    <dbReference type="FunCoup" id="A0A1E5R798">
        <property type="interactions" value="816"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R798"/>
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    <dbReference type="OrthoDB" id="10249245at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11286">
        <property type="entry name" value="ADF_cofilin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000060">
        <property type="entry name" value="Cofilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017904">
        <property type="entry name" value="ADF/Cofilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11913">
        <property type="entry name" value="COFILIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="10">
        <location>
            <begin position="5"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ82790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="143" mass="15873" checksum="F6C8779EFAACE00F" modified="2017-01-18" version="1">MSRSGVAVADESLAAFNDLKLGKKYKFILYGLNDDKTKIVVKETSTDSDYDNFLEKLPENDCLYAIYDLEYEISAGEGKRSKIVFFTWSPDTAPIRSKMVYASSKDSLRRALNGVSTDIQGTDFSEVAYETVLDKVSKGAGSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7I0</accession>
    <name>A0A1E5R7I0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ubiquitin-40S ribosomal protein S27a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3149</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the eukaryotic ribosomal protein eS31 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the ubiquitin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7I0"/>
    <dbReference type="FunCoup" id="A0A1E5R7I0">
        <property type="interactions" value="1133"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7I0"/>
    <dbReference type="InParanoid" id="A0A1E5R7I0"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031386">
        <property type="term" value="F:protein tag activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000008">
        <property type="entry name" value="Ubiquitin-40S ribosomal protein S27a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002906">
        <property type="entry name" value="Ribosomal_eS31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038582">
        <property type="entry name" value="Ribosomal_eS31_euk-type_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01599">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01402">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="9">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ82857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="149" mass="17043" checksum="3238E2A83737A717" modified="2017-01-18" version="1">MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGGKKRKKKVYTTPKKIKHKHKKVKLAILTYFKVDSEGKVTKLRKECEHCGAGVFLADHKDRLYCGKCHTTYKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7L9</accession>
    <name>A0A1E5R7L9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">U3 small nucleolar RNA-associated protein 13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3191</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7L9"/>
    <dbReference type="FunCoup" id="A0A1E5R7L9">
        <property type="interactions" value="1103"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7L9"/>
    <dbReference type="InParanoid" id="A0A1E5R7L9"/>
    <dbReference type="OrthoDB" id="5414888at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013934">
        <property type="entry name" value="Utp13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854">
        <property type="entry name" value="TRANSDUCIN BETA-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854:SF15">
        <property type="entry name" value="TRANSDUCIN BETA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08625">
        <property type="entry name" value="Utp13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="152"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="401"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="510"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="552"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="594"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="637"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 13 C-terminal" evidence="6">
        <location>
            <begin position="690"/>
            <end position="830"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ82894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="834" mass="93167" checksum="A94658477EE8294E" modified="2017-01-18" version="1">MSAGLKTVYTNVDNLSPFYAAGGSIASISANGKVFASTVADQVNILSVSVEEGNSRSKPVLKLLEKIENEDEQEITALQVTHDGKYLCYVNQAQLVTIYDISNTKIIKSFKVSSPCYVMCNDENSSNLLAIGGTDGSIIIYDIKNGYTTHSFKGHGGIISSLKIYGELEDLENWLLISGDTNGAVKVWDLVKRKCLHTLQEHTSPVRGLDIVRLDDTGNFKVLSGGRDDILNVYSLFNGKNKKKTLKLENTLPVHQQVEACGFLSADVCYCAGGDALFQLVDLQKMSIFKKTAPLIEELFIVGVLPILNKTQLYLVLSDQTLFNINVEDFASTENGETQKELIGSSEETYIPISFKIAGNHGTIADMRLVGPNLDKLALATNSPTLRVVPYETNKYEVEMYEGHKDLLNSLDATEDGLWIATASKDHVAILWKYSSDTDEFEPYCKFVGHSASISAIALPNVSQHQDSKQPPNFVITASNDLTIKKWKVPSISTSSNGNIHEVKTSVYTRKAHDKDINAIDVSPNDSIFATASYDKTCKIWSVEDGELVATLTGHKRGLWDVSFCQFDKLLSTTSGDKTIKIWSLENFRILKTLEGHTNAVQRSFFINKNKQIVSSGADGLIKIWSLEDDGECLKTLDAHNNRIWSILGKNDGEEIISADADGFIQFWRDNSEQEIQQNLEKQKQKVEQEQSLQNFLRNGDWINAFLLAMQLDHPMRLYNVLNQSLKLNDGQGDFIFNEELDGAIGMLEDDQILRLMKRCRDWNTNAKSHLIANKVIKCITKSYNISKLSEIPGIISVINSIIPYTQRHYSRVDNLVEQSYILDYSLIEMDNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7Q1</accession>
    <name>A0A1E5R7Q1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cell division control protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3146</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7Q1">
        <property type="interactions" value="530"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7Q1"/>
    <dbReference type="InParanoid" id="A0A1E5R7Q1"/>
    <dbReference type="OrthoDB" id="5977743at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000093">
        <property type="entry name" value="Cell division cycle-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033308">
        <property type="entry name" value="PGAP5/Cdc1/Ted1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315">
        <property type="entry name" value="METALLO PHOSPHOESTERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315:SF4">
        <property type="entry name" value="METALLOPHOSPHOESTERASE, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="388"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="491"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="7">
        <location>
            <begin position="126"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ82901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="512" mass="59112" checksum="E9500B985B621510" modified="2017-01-18" version="1">MFKRGGGHGKDSDSELPLTNGARRTLKPKPMRLGKYSRLGIGSRMRLPWKHLISLFLLWLFSVQYFERNYVKSTIKSCSWNKWESWDKNAQPYRAALFGDPQIMDAHSYPGRPVIINLLTRKILDNYHRKNWRYIQNILSPNANLFLGDLFDGGRYWDDETWMEEYKRFNSIFEKKEYKMNIMSLPGNHDIGFGDTVIESSYNRFNTYFGETCSRYDLGNHTFYIVDTISLSDTKLPHLSDKVHEFLDNVDRDHLKTHPRIMLTHVPLHRNPSIQTCGPKRESKKDFPLMKGDQYQTVIDQDLSREVLEKIEPDLIFSGDDHDYCEITHQYTVGNTVQEAKEITTKSCAMNMGIAKPGVHLLSLYNPTNSLTGEKTYETEXCYLPSPYVPIICYITLGVFSAVYLILVVTCPKFFNNKVIKKLQTICQKHSGNKHAIAAETKESLLPQFAHSSVVKDKATNGFIGKIFNFFKDVLDELVVQTEEASKASSLFVSALIMSVSISFLFYWYYSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7R1</accession>
    <name>A0A1E5R7R1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Gluconokinase</fullName>
            <ecNumber evidence="3 9">2.7.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3233</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>D-gluconate + ATP = 6-phospho-D-gluconate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19433"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18391"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Carbohydrate acid metabolism; D-gluconate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the gluconokinase GntK/GntV family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.12" evidence="3 9"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7R1"/>
    <dbReference type="FunCoup" id="A0A1E5R7R1">
        <property type="interactions" value="1112"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7R1"/>
    <dbReference type="InParanoid" id="A0A1E5R7R1"/>
    <dbReference type="OrthoDB" id="275177at2759"/>
    <dbReference type="UniPathway" id="UPA00792"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046316">
        <property type="term" value="F:gluconokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02021">
        <property type="entry name" value="GntK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059117">
        <property type="entry name" value="APS_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006001">
        <property type="entry name" value="Therm_gnt_kin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01313">
        <property type="entry name" value="therm_gnt_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43442">
        <property type="entry name" value="GLUCONOKINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43442:SF3">
        <property type="entry name" value="GLUCONOKINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01583">
        <property type="entry name" value="APS_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="APS kinase" evidence="10">
        <location>
            <begin position="4"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ82950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="259" mass="29436" checksum="B418749EACE58102" modified="2017-01-18" version="1">MERPTVVVIGGTAGTGKSTVADSLVGLLRLKYPSAEFLEGDSLHPPANVAKMTAGVPLHDEDRWGWLEKVSEESTANAKKNDGISIITCSALKKKYREYISNHSKETTFFFIMIYASKEEIKKRLTRREGHFMKANMLESQFADLELPMSIEQEPESRVITVDGRLQNHVLLNVFQMAFDLLKFRPLEVQIWEIFHLYRDETHPDGRDADFQGVHVDLIINELSWVHVASLKNELRKMREAGKLCNPDGAGQYYVVNKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R839</accession>
    <name>A0A1E5R839_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">L-azetidine-2-carboxylic acid acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3408</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R839"/>
    <dbReference type="InParanoid" id="A0A1E5R839"/>
    <dbReference type="OrthoDB" id="10264707at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052742">
        <property type="entry name" value="Mito_N-acetyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43138">
        <property type="entry name" value="ACETYLTRANSFERASE, GNAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43138:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE ACA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ83054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="243" mass="27705" checksum="194819C0922F75F1" modified="2017-01-18" version="1">MSASSFLXNGPMFPTNAQLDQKIKPLSFLLADGAXTATAFPVYDISEVPESLVSYMCTNFSKEVQDGLTYPAYDITCKEFREYWFFSFCAIVLKGKLESLDPHQDHDADYWKENFLGTFYVKPNYPGRCSHNCNAGFFIPHSQRGQKLGRKLGQLYLKFAPKLGYSYSVFNLVFVSNPASWRIWDRLKFQRIGFVPNVAVLGKDQKSVKNGEDSREYKKTDAIIFGKDLTTVEADIFDDFVQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R867</accession>
    <name>A0A1E5R867_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cell division control protein 31</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3451</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R867">
        <property type="interactions" value="913"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R867"/>
    <dbReference type="InParanoid" id="A0A1E5R867"/>
    <dbReference type="OrthoDB" id="343296at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000001">
        <property type="entry name" value="Calmodulin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050145">
        <property type="entry name" value="Centrin_CML-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23050">
        <property type="entry name" value="CALCIUM BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="63"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="147"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="183"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ83084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="214" mass="23871" checksum="31884FD22611D892" modified="2017-01-18" version="1">MNRNQTSPTPKSHLGGNNGNGIYRNNNGNNINTXSKFGTKADKGNGSFNQAVSDNGDGQLLDDQKAEIYEAFSIFDLNSDGFLDYHELKVAFRALGFDLSKRELLDILEMFGTSSQTNQLNAGYTPSRLIGYDDFYSVAATKILERDPLEEIKRAFTLFDADNKGMITFENLKQVANDLGEKLSDEDIHKMINEFDLDEDGGINEEEFIAICTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R887</accession>
    <name>A0A1E5R887_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UPF0651 protein, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3339</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R887"/>
    <dbReference type="FunCoup" id="A0A1E5R887">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R887"/>
    <dbReference type="InParanoid" id="A0A1E5R887"/>
    <dbReference type="OrthoDB" id="10064411at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019180">
        <property type="entry name" value="Oxidoreductase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039251">
        <property type="entry name" value="OXLD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21193">
        <property type="entry name" value="OXIDOREDUCTASE-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21193:SF3">
        <property type="entry name" value="OXIDOREDUCTASE-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09791">
        <property type="entry name" value="Oxidored-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidoreductase-like" evidence="2">
        <location>
            <begin position="87"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="247"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ83120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="258" mass="30110" checksum="3B74386A1361D961" modified="2017-01-18" version="1">MYTTGRHCARLLFTRSSRTTVEHFHQGAPRKMTFQGNIAGLKMTEEERMATLFGGDKNKYTADKLQGSAQETSRFISRSHDYYQIGSIKIPYKPIEPDNCCMSGCVNCVWLMYQDDLQNWKHQRRIAAKEINKTDEVWPENLKPPIHLLNERNIPKSMKKLKEKASKKRRLSTFQFPPQLNQTELEKQAAEQEAREADEAAAETEAEEDVTPEFIKQFTRFEKQKAAEKLHKLQERQLHPDTSSPVQQQQQQQQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R897</accession>
    <name>A0A1E5R897_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Nucleolar GTP-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3320</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Involved in the biogenesis of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Nucleus</location>
            <location evidence="2 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. NOG subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R897"/>
    <dbReference type="FunCoup" id="A0A1E5R897">
        <property type="interactions" value="1351"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R897"/>
    <dbReference type="InParanoid" id="A0A1E5R897"/>
    <dbReference type="OrthoDB" id="415015at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902626">
        <property type="term" value="P:assembly of large subunit precursor of preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01897">
        <property type="entry name" value="NOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1190:FF:000001">
        <property type="entry name" value="Nucleolar GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000496">
        <property type="entry name" value="Nucleolar GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024926">
        <property type="entry name" value="NOG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041623">
        <property type="entry name" value="NOG1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010674">
        <property type="entry name" value="NOG1_Rossman_fold_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012973">
        <property type="entry name" value="NOG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45759">
        <property type="entry name" value="NUCLEOLAR GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06858">
        <property type="entry name" value="NOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17835">
        <property type="entry name" value="NOG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08155">
        <property type="entry name" value="NOGCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038919">
        <property type="entry name" value="NOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="OBG-type G" evidence="10">
        <location>
            <begin position="168"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="460"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="589"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="460"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="589"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="610"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR038919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ83135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="659" mass="75074" checksum="CDC22FCB9A558D2F" modified="2017-01-18" version="1">MQLSWKDIPTVPPANDLLDIVLNRTQRKTPTVIRPGFKITRIRAFYMRKVKFTAEGFDEKFEDVIKGFPNINDVHPFHRDLMDTLYEKNHYKISLAAVSRAKTLIEQVARDYVRLIKFGQSLFQCKQLKRAALGRMATIVKKLKDPLQYLEQVRQHLGRLPSIDPNTRTLVICGYPNVGKSSFLRAITKADVEVQPYAFTTKSLYVGHFDYKYLRFQAIDTPGILDRPTEEMNNIEMQSIYAIAHLRSCVLYFMDLSEQCGFTIEAQVKLFHSIKPLFANKSVLVVINKTDIIRPEDLDEERSAMLQTVVEAQGVEVMTTSCVQEDNVMEVRNKACEKLLTARIENKLKSTSRVNNVLNKIHVSRPQQRDDIERKPYIPESVQNLAKYDPLDPNRRLLARDIEAENGGAGVYNLNMKDKYLLEDDDWKNDVMPEIMDGKNVYDFLDPEIAAKLQALEEEEEKLEQEGFYDSDNEDAEGDEDMGMSGESFKGYSAAETAEIKEKARWIKDKQKKMIISSRNRKALKNRGVMPRSKLAKSFSEMEKHMDSLGHDMATLQDKQNDAQIKNRYVETGADVVLNASSASLAQAGSNGGTLTQSDRSLDGVADGAMRSKAERVLKLQRRERNRQAKIGEADRHATAALPKHLMSGKRGNGKTDYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R8G6</accession>
    <name>A0A1E5R8G6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Autophagy protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3411</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in cytoplasm to vacuole transport (Cvt) and autophagic vesicle formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Conjugated with ATG12.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Preautophagosomal structure membrane</location>
            <topology evidence="1 6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ATG5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R8G6"/>
    <dbReference type="FunCoup" id="A0A1E5R8G6">
        <property type="interactions" value="346"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R8G6"/>
    <dbReference type="InParanoid" id="A0A1E5R8G6"/>
    <dbReference type="OrthoDB" id="272162at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034274">
        <property type="term" value="C:Atg12-Atg5-Atg16 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044233">
        <property type="term" value="C:mitochondria-associated endoplasmic reticulum membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061908">
        <property type="term" value="C:phagophore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019776">
        <property type="term" value="F:Atg8-family ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.190">
        <property type="entry name" value="Autophagy protein Apg5, helix rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007239">
        <property type="entry name" value="Atg5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048940">
        <property type="entry name" value="ATG5_HBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042526">
        <property type="entry name" value="Atg5_HR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048939">
        <property type="entry name" value="ATG5_UblA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042527">
        <property type="entry name" value="Atg5_UblA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048318">
        <property type="entry name" value="ATG5_UblB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13040">
        <property type="entry name" value="AUTOPHAGY PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13040:SF2">
        <property type="entry name" value="AUTOPHAGY PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20637">
        <property type="entry name" value="ATG5_HBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20638">
        <property type="entry name" value="ATG5_UblA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04106">
        <property type="entry name" value="ATG5_UblB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0072">Autophagy</keyword>
    <keyword evidence="3 6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="4 6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Autophagy protein ATG5 UblA" evidence="9">
        <location>
            <begin position="12"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Autophagy protein ATG5 alpha-helical bundle region" evidence="8">
        <location>
            <begin position="139"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Autophagy protein ATG5 UblB" evidence="7">
        <location>
            <begin position="200"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ82823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="268" mass="31266" checksum="24F27FE782BF31A0" modified="2017-01-18" version="1">MSTQVKEIRRLCWNGSVNVQVVLDQSLSLEGLSVEERTLLFKLNRNSYIMCYFDGIIAKFSNLIKNKEFAKQTGKWGFSLTDHQDSPLPWNYPLGVLFDTYSEHVKKYEANMIQTFNLKLVHNDIVTFKNFLPLYDGLKQVRLYWMHQWKQACYIMHGSAKRVMSLTVQEVDLFWEACFNGISENYEKTSTKIVNYSKRIPLRICSPTQIMQPVCTSETVLRKLLLQELNAECETVIIQGVEISTLHLVRDLYTRFASFDGFLYIILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R8U6</accession>
    <name>A0A1E5R8U6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Prephenate dehydrogenase [NADP(+)]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3342</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R8U6">
        <property type="interactions" value="250"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R8U6"/>
    <dbReference type="InParanoid" id="A0A1E5R8U6"/>
    <dbReference type="OrthoDB" id="5399569at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008977">
        <property type="term" value="F:prephenate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004665">
        <property type="term" value="F:prephenate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3660.10">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050812">
        <property type="entry name" value="Preph/Arog_dehydrog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003099">
        <property type="entry name" value="Prephen_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012385">
        <property type="entry name" value="Prephenate_DH_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21363">
        <property type="entry name" value="PREPHENATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21363:SF0">
        <property type="entry name" value="PREPHENATE DEHYDROGENASE [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036510">
        <property type="entry name" value="PDH_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51176">
        <property type="entry name" value="PDH_ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate/arogenate dehydrogenase" evidence="2">
        <location>
            <begin position="18"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="468" mass="53480" checksum="A5A0E250BDFE60F1" modified="2017-01-18" version="1">MTTKKYTEEIARQWKETKKIGIIGLGDMGLLYAQKLSANGWQVVCCDRPEIFESVTVPKYXDKTKYSFKLVKDGHLLSRECDYIIYSVEAANLDKIVQVYGPSSKMGAIVGGQTSCKNGEIQAFEKYLPQDVDIITVHSLHGPKVNTKGQPLAIINHRVSKTLNSDFVDCVMDCFESKKVHLTAEEHDQITADTQAVTHAAFLSMGRAWYKRQVYPWQSTSNNGAKWSGGLENAKINISLRIYSNKWHVYAGLAITNPAAHKQILQYSQSATEIFTLFMENKKEELTERLFKAKNFVFKKDVLNKQKVSLLLDDSMLQRFSLNDSRYIDDQHNVQERPSSVEQKIISNNHLSLLSIVDCWYQLGIIPYDHMICSTPLFRIFLGVSEYLFSNEKLLLQSIEAGCQNTEFRGDDLEFVIAARNLSMMVSHGDFELYRKIFEETQEFFKPMLPEATRIGNQMIKTILENTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA13</accession>
    <name>A0A1E5RA13_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2997</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RA13"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184661" evidence="2">
        <location>
            <begin position="23"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="162"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ83746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="590" mass="58248" checksum="2A5309362D0CB395" modified="2017-01-18" version="1">MKAVQILSFSAAFAFFTQQAAAVPVSSNIVVPTIQLENPKTTIVSSNLTSTPSISSDANPTSVNIISFPTFEFLDPKTTISSSTPTPGFIVVPTIQLVDPRTTISPASAASSLSSAAPSDVISVDTIQLLDPRTTLTESSTTSAAPSDVISVDTIQLLDPRTTLTESSTTSAATSTSSAGSSTSESSAASGLISVSTIELIESSSVVNPTSSVVNFSLAAPSASATPSNVTGVDALVASFVVPFNLALPSSSVVVSSVAPSSSIEIDAYVASSANSSVVASSVATFSVVASSVAASSVAASSVAASSDAASSVIGSSTASTVSAVSSTNEISISSAALVESTTSSGSASTISDNTSSSKSASATSTYTKTIATTVTTSYPTFVNGNLTFTTETIAFTSTTTFCPESATSSSLTPATETSVTASTPTFVNTFASMLNGLSFANTTTVDNIAAVSTVSTTTLAPSSGAVSNAAGTVASSQVASSQVASSQVASTQVASSQVASSQVASSQVASTQVASXQVASSQVAPSTVVNPPSVSTLTNSVVTSGSSSVKASATVAVAQTNAGVNNKVTFSTLMTLVISSSLATLFMFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA25</accession>
    <name>A0A1E5RA25_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Isoleucine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="3">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Isoleucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g2991</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Ile) + L-isoleucine + ATP = L-isoleucyl-tRNA(Ile) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:9666"/>
            <dbReference type="Rhea" id="RHEA-COMP:9695"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA25">
        <property type="interactions" value="1167"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA25"/>
    <dbReference type="InParanoid" id="A0A1E5RA25"/>
    <dbReference type="OrthoDB" id="1706657at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07961">
        <property type="entry name" value="Anticodon_Ia_Ile_ABEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00818">
        <property type="entry name" value="IleRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000004">
        <property type="entry name" value="Isoleucyl-tRNA synthetase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000023">
        <property type="entry name" value="Isoleucyl-tRNA synthetase,cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000050">
        <property type="entry name" value="Isoleucyl-tRNA synthetase,cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02003">
        <property type="entry name" value="Ile_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033709">
        <property type="entry name" value="Anticodon_Ile_ABEc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023586">
        <property type="entry name" value="Ile-tRNA-ligase_type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780:SF1">
        <property type="entry name" value="ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780">
        <property type="entry name" value="SOLEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19302">
        <property type="entry name" value="DUF5915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 13 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="14">
        <location>
            <begin position="14"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="15">
        <location>
            <begin position="691"/>
            <end position="850"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ83744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1070" mass="123891" checksum="BE9474C0666E56DB" modified="2017-01-18" version="1">MSESHFSFPREEEKILQNWNDIDAFHTSLKLTEGKPEFSFFDGPPFATGTPHYGHILASTTKDIVPRYATMNGYHVERRFGWDTHGLPIEHIIDKKLKITCKDDVYKFGIDNYNNECRKIVMTYADEWRKTIGRMGRWIDFDNDYKTMYPTFMESIWWAFKQLYEKDQVYRGFKVMPYSTGCTTPLSNFEAQQNYKDVYDPAVTVGFDSVDEEKTQFVAWTTTPWTLPSNMXLCVNPDYEYVKIYDEKKDKYFILMESLIKTLYKKPAQEKYKVVQKYKGSDLVGLKYKPLYPYFVDGFGEKAFRIISDSYVTNDSGTGIVHNAPAFGEEDYNACLKNGIISEEDKVPNPLDDAGKFTEEIGDYKGVYVKDADKLIIKKLTETGNMLLASQVKHSYPFCWRSDTPLIYRTVPAWFVRVKPVIDKMLESVAASNWVPNNIKEKRFSNWISNARDWNVSRNRYWGTPIPLWVSEDYDEIVCIGSVEELKELSGVQDITDLHRDKIDHITIPSKKGKGVLRRVEEVFDCWFESGSMPYASQHYPFENQEKFVERVPANFISEGLDQTRGWFYTLSVLGTHLFGEIPFKNVIVSGIVLAADGRKMSKSLKNYPDPNIVLEKYGADALRLYLINSPVLKAETLKFKEEGVKEVVSKVLLPWWNSFKFLEAQVQLLKKEQNEDFTFNPDVASENVMDRWMLASLQTLVQNIHIEMKAYKLYNVVPKLLSFIDQLTNWYIRFNRKRLKGENGTDDCKKALNTLFEALFTFVRAMAPFAPFLSDNIYLRLKEYIPKETLAKYGEDDRSVHFLSYPTVREELFDADIELAVSRMQTVIELGRNVREKKAISLKTPLKTMIILHGDQKYLDHVEALKGYIVEELNVRDLIITSDEAKYNVQYSAVADWPVLGKKLKKDAKKVRDALPKLTSXEVQQYQKTGEITVADIKLASGDLTVQRGLSEDEIKEGHEVRTDQDVLIIMDVKVYPELKTEGSAREIINRIQKLRKKVGLVPTDDVIVQYELVKDEMDFENVVKEHYNMITKTVKSDFSKLDNSKSEIIADEEQSINETIFKLKIFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA34</accession>
    <name>A0A1E5RA34_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2549</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA34">
        <property type="interactions" value="851"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA34"/>
    <dbReference type="InParanoid" id="A0A1E5RA34"/>
    <dbReference type="OrthoDB" id="1611972at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000079">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1430.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.960.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000351">
        <property type="entry name" value="Ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.960.10">
        <property type="entry name" value="Ribosomal protein L3, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045077">
        <property type="entry name" value="L3_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044892">
        <property type="entry name" value="Ribosomal_L3_dom_3_arc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_uL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_uL3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6 7" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ83758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="387" mass="43797" checksum="A94E48AE55881A43" modified="2017-01-18" version="1">MSHRKYEAPRHGHLGFLPRKRAESVRGKVHAFPKDDQTKPVALTSFLGYKAGMSTIVRDLDRPGSKFHKREVVEAVTVVDTPPVVVVGVVGYVETPRGLRSLTTVWAEHLSDEIKRRFYKNWYKSKKKAFTKYSAKYAQDGEAIERELARITKYCSVVRVLAHTQVRKTPLXQKKAHLAEIQLNGGSVADKVQWAKEHFEKTVSVDSVFEQNEMIDVVAVTKGHGFEGVTHRWGTKRLPRKTHRGLRKVACIGAWHPSHVMWTVARAGQRGYHSRTSINHKIYRVGKGEDAGNASTEFDRTQKTITPLGGFVQYGVVNNDFVMVKGCIPGTKKRIVTLRKSLYTHTSRRALEQVTLKWIDTASKFGKGRFQTPAEKHAFMGTLKKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA66</accession>
    <name>A0A1E5RA66_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Regulatory protein SWI4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3017</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA66">
        <property type="interactions" value="1036"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA66"/>
    <dbReference type="InParanoid" id="A0A1E5RA66"/>
    <dbReference type="OrthoDB" id="3972162at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030907">
        <property type="term" value="C:MBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033309">
        <property type="term" value="C:SBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.260.10:FF:000006">
        <property type="entry name" value="Swi4p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.10">
        <property type="entry name" value="Transcription regulator HTH, APSES-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036887">
        <property type="entry name" value="HTH_APSES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018004">
        <property type="entry name" value="KilA/APSES_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051642">
        <property type="entry name" value="SWI6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003163">
        <property type="entry name" value="Tscrpt_reg_HTH_APSES-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828">
        <property type="entry name" value="ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828:SF7">
        <property type="entry name" value="REGULATORY PROTEIN SWI4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04383">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01252">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54616">
        <property type="entry name" value="DNA-binding domain of Mlu1-box binding protein MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51299">
        <property type="entry name" value="HTH_APSES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HTH APSES-type" evidence="6">
        <location>
            <begin position="39"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="465"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="648"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="179"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="244"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="589"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="902"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="970"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="257"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="295"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="615"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ83784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1142" mass="127972" checksum="EEE3498AE1571DFC" modified="2017-01-18" version="1">MKTPIGSGVKNRGKNSFSVGKNGVXGMIAELGTIDNPIIEKAKYAGVEVFECYTKKPKGQLVMRRVRDDWINITQIFKLAKFSKAKRTRILETESKNIRHEKIQGGYGRFQGTWIALEDAKYIIEKYQLQNPVATKLVSFVEDPNNPIPLREKLHALKKQMKLNALEASNGYDKIITSSSSLSSSSYDKKNPASSINGVNEEVNGVPKSSSLETPLKFQQHLANSAEGNFGTNSNNKKRSIAQDQLQPHNENDDTESDRTITXECTTEERSTVASPYKRTKSNSQSPSKDKDNLHTQNGKFSKNSMSQQNGVNGTNGTTKPLQFFPYPTSNMKRFHQTPVINKKTLSRNASTSTKKWMETPHGITHSELKAANQSHILDSNTMNPSINSPLMTYQSRVTNITRNPDGGITSTPFVHPISTADYQHLLLKFLSSSNSAASQEEIVFPKELYFPPPDFNPNFILDGEGNSTLHWAVSMGNLPLVQFLLTNLKADPLVCNAKGFNCITKACFYNNCYKNQVFVDMLLCLAQCLISPDKNGRLPLHYLMELSVNMAKEQEVISYYLDCVLQVVSIPFPQSEISANGIHSTHTDLADPLKTNINNEGRNGKRKRHPSPSLDEKDHVGNGLDKSHNSHMMLNLLPTVLNYQDELGNTPLHVAALNMNFDLCSKLIAMGGXQDLLNYENCSVSDILHKAQFLLSSETTGPHSAPQPRFPKSSMQNGTEFYLNKNHHSKDLGQHPKIEFPEFNVNEYQNPQLNNVTTSDFANEKFPIVYPSNGSNHKNDKLSFSNXVATKNVYELKNKVPXSKVSALEKHEIGATGGTQDNILSGSNIVPSLSSVDEQLLGKSHHHNELSEALESGDFSAYGTHVISPDPNGNLMGNDSSNLQSLQRYPHHEMSFISKEEAQEDKQLLPPSTPSKPNGMGSANSALYSGGNMSVQSNAMFTKVHKLNNMVINFTNQLDEVYFRRQENKLTFRRNLEEAEQKLQVYKDHARAFEKHRSEIAEQEKRLLAKQKELERFKLKYFNSIEKSQGLRFATAFVNMGDHLPEKALDEPTTKEFEQKQKRITYSIKISLAQLKRNKKIAKICSTKALSNNEKIFKYRKLIGKSVKNIDSKLDGIEKDLRKTEEISTVSFSLPQPKESG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA94</accession>
    <name>A0A1E5RA94_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation factor 1 alpha-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g2511</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Dom34-Hbs1 complex, also named Pelota-HBS1L complex, composed of dom34 and hbs1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA94">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA94"/>
    <dbReference type="InParanoid" id="A0A1E5RA94"/>
    <dbReference type="OrthoDB" id="342024at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990533">
        <property type="term" value="C:Dom34-Hbs1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000070">
        <property type="entry name" value="Translation elongation factor EF-1 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000204">
        <property type="entry name" value="Translation elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="12">
        <location>
            <begin position="445"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="95"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="117"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="238"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00063537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00074866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ83825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="896" mass="99937" checksum="27C2488982F8E0CC" modified="2017-01-18" version="1">MPAYDYDDDDLDYSDDGGNSPMNFKTERDLDDYLNDEEYDMMGLIFPEVLDYLKSNHYYGYDNLTVKLAIFDSNFNIDEAKLELSEVLKQKKPEISKPIKKTPNLPKKKKLALAKQGRQNTAHQDPSNLTADSAPKKLSLADKLKLKREQGLLSSQQNNQPVSNENHSSPLSLAEKLRLKKSAGSSHADISASITVDAPTKQNTKETTSLVNKLQKMSLVERLQNKKTVNQGISAQTKNFSSKGKNFSSSLDDNNDTSIHSEEIQKSENKKQLSWFESLKNQLFENRSVDPTKNAPNLHNKAHANLRVSNIIVEIDEKTAVNTKSVSKNKREKRKRPFNEIFTIYYPNTNIKKLAIENFNKPSPDDVIINAQQSAFESVNKQVEGLSIKDGNVEENTKAIDMNQPGKKDDEELVTVIHKYKKVXEPTKPKEKIDVVASIQEYKKHPHISFVVLGHVDAGKSTLMGRLLYDVGAVDTKLIRKLQKESEIIGKSSFHLAWVMDQTSQERERGVTVDICTSNFATKNCSFTIVDAPGHRDFIANAISGISQADVAILCVDCSTDAFESGFNLDGQTKEHAILARSLGLKHIVVAMNKLDTVDWSKQRFNEIKDQLSVFFHMIGLDVGKQISWVPCSGLNGDGVVKAQWSSPKQNWYTGSSLVQELENVAHAFVNEDENLNANIXSFESSGEPTSPFLFNVLEVIASSKNNECXVSGKIEQGFIQPGETITLYPSEQSCVVDQVYHQQDDRSLLKLGIEGDFVYLKLKNANSDDIHSGDLATIVGNELHNSKSFEVQLLTFDMPRPLLPGTPLLIYRGSGEQPANVKKLIKILDKNNPNKVLKKKVKHLGSNQLAVVELEVDSKSQDIPMLTNAQNKNLSKLVVRKEGKTIGAGVVTQLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAD9</accession>
    <name>A0A1E5RAD9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Mitochondrial aspartate-glutamate transporter AGC1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g2845</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAD9">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAD9"/>
    <dbReference type="InParanoid" id="A0A1E5RAD9"/>
    <dbReference type="OrthoDB" id="2161at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015183">
        <property type="term" value="F:L-aspartate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043490">
        <property type="term" value="P:malate-aspartate shuttle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051028">
        <property type="entry name" value="Mito_Solute_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45678:SF9">
        <property type="entry name" value="CALCIUM-BINDING MITOCHONDRIAL CARRIER PROTEIN ARALAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45678">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="561"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="657"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="811"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="190"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="780"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="903"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="791"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="903"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="13">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ83864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="992" mass="114599" checksum="19F447C1ACCF7B7A" modified="2017-01-18" version="1">MELENKSRSRKKLKQIEIFNRYAKQLDDSTSRVVLDYDGFIQILKDSTSFFHMKGCXKNKTNFNKLSEKDINETKACDNTPSRSMFTDHKYNLNHIPMDTMGLIFFAIDEQNKGYLTMQDWMHFNNLLENNNYQHILFYEFLQKNNSKNSKYLQSITYTNKFLTFDSLFIDLQMLKNLLTDVDDYTTESSNLNSEKASATASSSSSSSSSNTNITNSVTKDDYEISENIPIDWQNLSLMDNYIADENHPQLLTLNSLISMVQNDLKVYKTRYYYNHYKKQDNHSGYSYINRNNLIEILKLQYSHKINNIIFQSIDLSNSHLIKCTNDKIPYNMYQDFIYLFDNFDILNQLLLNYCSKNNIVLDTSLKNYQNRVITKKEFMNILTKNSYNKVNNIIQFSPSQINILFSIVANAKNKELTLLLKEQEMLEQENIEKQILHEKYIKDEFMRNELLETTKREAMELFEQNYKELMEQDSSHNSKPMYTNSKFITYLFDKLLVSPIDRPKNSTAINTHSLNYLNLHDFFKILNPNYLNDLIHLIELKEKTINENKIQSNFFFYPIFDNITNFIIGSVSGIIGATIVYPIDIVKTRMQAQQGNTLYKSYLDCLLKIVKRESFKGLYSGLKPQLIGVAPEKAIKLTVNDSMRSFFTNYKNSNNLLLVEEIISGATAGACQVIFTNPLEIVKIRLQLVNQLIENNQNNKIITSEMKEAITQKLPLQKPKGATAISIVKELGLQGLYKGSLACMARDIPFSAIYFPTYAHVKKFLFNFDPNASSTVSHXKPLLHDHNETGSDSGNNGDNTDNSRLYRNKLKTWELLISGAIAGMPAAYFTTPFDVIKTRLQMDSKTNKNKYTSIFQTAKLIWKEESFKSFFKGGTARVLRSSPQFGFTLAAYEIFQGFLPSSSPPPTPPTVSIPDSPTSTDXNNAHTNIGKPVKQNFQLQRKEPNSSYSDIDIDYYYKSCNIVKTFIDLDPTFRNFDHQVYEKLSSAQRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAE6</accession>
    <name>A0A1E5RAE6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">mRNA m(6)A methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.348</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2819</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>an adenosine in mRNA + S-adenosyl-L-methionine = an N(6)-methyladenosine in mRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55584"/>
            <dbReference type="Rhea" id="RHEA-COMP:12414"/>
            <dbReference type="Rhea" id="RHEA-COMP:12417"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74449"/>
            <dbReference type="EC" id="2.1.1.348"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MT-A70-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.348" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAE6">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAE6"/>
    <dbReference type="InParanoid" id="A0A1E5RAE6"/>
    <dbReference type="OrthoDB" id="10262526at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036396">
        <property type="term" value="C:RNA N6-methyladenosine methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001734">
        <property type="term" value="F:mRNA m(6)A methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007757">
        <property type="entry name" value="MT-A70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829:SF7">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05063">
        <property type="entry name" value="MT-A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51143">
        <property type="entry name" value="MT_A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184690" description="mRNA m(6)A methyltransferase" evidence="8">
        <location>
            <begin position="19"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="544"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="551"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="575"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ83879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="584" mass="67063" checksum="B37AE6F62A70E866" modified="2017-01-18" version="1">MYDFCTLGLISFLIQSHAVIEGYPICGNIKDLMIVYFSANDHLSNKIENSKKSFEACHEGFRKALQALKDIDPSCFDITDDVIHGEKIININEETLEIVYNCIHRRELSTTQATQQLSHGQPLLIKTSDSTSEASNADLSLEXRLLTLIEMDTTSEDNANNKMPVDSTSPNAHLYSRLESTLKKETAFKSLYREKIVSHANPKPYTSVCSDSEHGKKILHFVKIHNNKISAVNDLRGITHIFXQTEXSCLQTHTHFIPNLKPHTDMSLGDCSYLDTCHKLNTCRYVHYIQCSPLLFRKNESFQKENKLLEASENPGLFTHGENTKRKELLPKQWIKCDIRKFDFRXLGKFSCVIADPAWNIHMNLPYGTCNDNELLAMPLNLLQDEGIIFLWVTGRSIQLGKESLEKWGYRVINEISWIKVNQLGRTIVTGRTGHWLNHSKEHLLVGIKGSPVWLNKQQXLDLIVSYTRETSRKPDELYGMCERLCGKHARKLEIFGRDHNTRPGWFTIGNQLXDSCVWELDVKTKHEKWLSGRAHENVAVKNKSQNYRNKPSEIFDAQKPRQASKKKAYLFQQHPRSVSSTFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAE9</accession>
    <name>A0A1E5RAE9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative vacuolar amino acid transporter YPQ2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2562</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-histidine(out) + L-arginine(in) = L-histidine(in) + L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:71063"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the laat-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAE9"/>
    <dbReference type="FunCoup" id="A0A1E5RAE9">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAE9"/>
    <dbReference type="InParanoid" id="A0A1E5RAE9"/>
    <dbReference type="OrthoDB" id="8048523at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034488">
        <property type="term" value="P:basic amino acid transmembrane export from vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000009">
        <property type="entry name" value="PQ loop repeat family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF34">
        <property type="entry name" value="LYSOSOMAL AMINO ACID TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="262"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="298"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="333"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="162"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ83874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="369" mass="40931" checksum="4D126278DE3328AD" modified="2017-01-18" version="1">MDSFLNIMNSTLNSTLPNIPPPNQIGVCPESSVFNVISTTAGSLAFISSMIAQFPQLIETYRDKTVEGVSNLLLLSWLGGDITSIIGAILTNQLPFQIVLAIYFLSNDLIICAQYYYYGIYHGNRLATAGHQHLDPSSVATKHSHLHHSHLHHHHHHHENNENSVTTRVSGPAQTPRKSILAGVLSFFSLSRAMPITSSMLQSSSAIVLASSTNNQLSALGMDAQTIGTFCSWIGALFYFCSRIPQLLKNYHRKSTDGVSPLLFYCTIFHNCSYGISIFTSCQFVYNEDKWGFVKNEAPFLLGSVGTLLFDFIYLYQHFVLYNEDTKLRRQLSRQGNRTPSFSGQSHGHIQSSDSDNANENSPLLISSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAG7</accession>
    <name>A0A1E5RAG7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">rRNA biogenesis protein RRP36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2780</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Component of the 90S pre-ribosome involved in the maturation of rRNAs. Required for early cleavages of the pre-RNAs in the 40S ribosomal subunit maturation pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Associates with 90S and pre-40S pre-ribosomal particles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
            <location evidence="1 9">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the RRP36 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAG7">
        <property type="interactions" value="580"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAG7"/>
    <dbReference type="InParanoid" id="A0A1E5RAG7"/>
    <dbReference type="OrthoDB" id="3973209at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009292">
        <property type="entry name" value="RRP36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21738">
        <property type="entry name" value="RIBOSOMAL RNA PROCESSING PROTEIN 36 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21738:SF0">
        <property type="entry name" value="RIBOSOMAL RNA PROCESSING PROTEIN 36 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06102">
        <property type="entry name" value="RRP36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 9" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 9" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="310"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="10"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="85"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="160"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="324"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ83898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="335" mass="39481" checksum="AEE5E8131ED0648E" modified="2017-01-18" version="1">MSFYFKNIRPGHEAEENYEKDNDHLNDILRQESSDEDDEAFGNAGGDDEDDGLSKVSFGSLKQAQDDYVSNERKTKKTSKPTXKTVDDEIKNEIKQKMQEKASLQPKMFKEESFDEDDSSNSGSXEEGLFEEEIASRKLQRKNEQXQRAPQRKGKKGSNHAPSEQSSKKPVSFIRDIPGLEINKKYKDSATYEDIRFDKSLGRSEDFNKVRSRYKFLDDYRAQEIKTLETSLKDKKFLSKINEYEHAKLEERLRSMKSKLQTLQNKDLENEIVKKYTNEINSKSKNKFYLKDSDKRKIINKYKFDHMKASQREKVMERKRKKQLGKEMRQFEFNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAK2</accession>
    <name>A0A1E5RAK2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ribosomal RNA-processing protein 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2496</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SURF6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAK2">
        <property type="interactions" value="342"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAK2"/>
    <dbReference type="InParanoid" id="A0A1E5RAK2"/>
    <dbReference type="OrthoDB" id="444809at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029190">
        <property type="entry name" value="Rrp14/SURF6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029188">
        <property type="entry name" value="Rrp14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007019">
        <property type="entry name" value="SURF6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14369">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14369:SF0">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15459">
        <property type="entry name" value="RRP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04935">
        <property type="entry name" value="SURF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal RNA-processing protein 14 N-terminal" evidence="7">
        <location>
            <begin position="8"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA-processing protein 14/surfeit locus protein 6 C-terminal" evidence="6">
        <location>
            <begin position="246"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="38"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="415"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="332"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="39"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="62"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="136"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="160"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="202"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="266"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="416"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="431"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="457"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF15459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ83936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="466" mass="53443" checksum="21C9F287BAC69243" modified="2017-01-18" version="1">MSNSLQERLQNNANSFEGLLSLIPAKFYYDDEDNNNQWKAKKQSKKQKNANKRMKLSSGIENDGDSSDDDDEEEEEEEEGADGESLEGEEQKQKVNILKTGSVKDXMDATEKKPAVIPGMKVKKLQEQQQQQQELQDGENGSEEEVLEQVVVVGDEPSDQNDGISNGDNSMMKDIIFDDEGNEMTQDFKKQQAQQGKKHQLKNTDSKEKKQKLDKLRSALQQKIQSLKEKRKAPGSSAQGAPVSREAILEQRKRKQELSQKKKLEADDESESASDSDSDNSSGDSSDDEQDYXDHDINADNVMFQNIQFSDGSKITSDLQRVRTIKKKGPANKDIKGHLKMLERKKQKLENKDELELIKLKEKEKWNKVMLHAEGVKLKDNEKLLKKSLKRKENQKKKSAVEWRERKQMVVTGLAERQKRREENLQIRKENKGKKRKDRTKFKKTLRGKKAGSIKRAGFEGKIKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAY4</accession>
    <name>A0A1E5RAY4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Vacuolar protein sorting-associated protein 74</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2690</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GOLPH3/VPS74 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAY4"/>
    <dbReference type="FunCoup" id="A0A1E5RAY4">
        <property type="interactions" value="548"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAY4"/>
    <dbReference type="InParanoid" id="A0A1E5RAY4"/>
    <dbReference type="OrthoDB" id="2189106at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031985">
        <property type="term" value="C:Golgi cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048194">
        <property type="term" value="P:Golgi vesicle budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3630.10:FF:000004">
        <property type="entry name" value="Probable VPS74-protein involved in protein-vacuolar targeting"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3630.10">
        <property type="entry name" value="yeast vps74-n-term truncation variant domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008628">
        <property type="entry name" value="GPP34-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038261">
        <property type="entry name" value="GPP34-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12704:SF2">
        <property type="entry name" value="GOLGI PHOSPHOPROTEIN 3 HOMOLOG SAURON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12704">
        <property type="entry name" value="TRANS-GOLGI PROTEIN GMX33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05719">
        <property type="entry name" value="GPP34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ84060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="336" mass="38466" checksum="FA331EC7C9009A38" modified="2017-01-18" version="1">MIHRRVNAAAQRSDDEEDHSNGVSEITNSLNTTKLSSTTQRDTTGSNQNRQLVIPELTLMEEVVLLGLKDEGYLSFWNDNISYALRGLILIELALRKKIRIIDDTARRRYDLSERLIEVVDNEKTGEALLDETLNLMFQDNKQPQSIAQWIDLLSGETWNLMKINYQLKQVRERLSKGLVDKGVLRTEMKNFFLFDMATHPINDVSCKESVKRRVLSALVSRNFSLNYNKYFSENVTFKVLRTLCLIGGASGANVLENVLTTLEYEKRDIGFDRAEEIVDQLSQWPFDLGKKTNTGVSINLNQELEQELEENAGNDLYLEIIAGVFEVFSRMDSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAY8</accession>
    <name>A0A1E5RAY8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pumilio y domain family member 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2617</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </evidence>
    <sequence length="813" mass="88233" checksum="AEDAFE1AE9483A10" modified="2017-01-18" version="1">MATPTEETITKALDNLEIDSEPIAHAGARILSGTTETLTKDAXDNAQQAHPYMGFPQMHHPLMNGGPLPPLPHMMAYPPPPSQYMMMDSNNGGEYAGNKPASSAGTDFAMFNPYTMMPPTSGSTTSPMTTDPFWAPANHGGLPPVSDLSVPKSTVGEMSYPRGASFAGPSSSALEDAIAGEDGVFDEQLGQPKSRRQTFHSGSGNAMFGSQQHPQSASIANPASSRTQSISLDGPSGAIFASTSSSNNTVDVDGKKEKTSELAQGFQQTSFPPAYPYNGPLLHPNPLYNGHPMSGSPFQPYGYPSFQSFSPIPSGVKPSGNEKDVVSVGXPSATNATGMGLPSDDFAAAGIIPPHIDSPANIPWMYGSPSPFPAGQASMMMPNNAGHKNGNLYGSKNYKMRAGKKFNNGEARSKNDTSRGKNDDQYNDGNYNRSYRAEHYNSMISASSKYNNATLADFVGGIYSLCKDQHGCRFLQKQLDIDGEAAASMIFFEIKDHIVELMVDSFGNYLIQKLLEISGQEQKLEIVTIAAPDIWKISFDPHGTRALQKLVECITTLKEVEIIQESLKGHIVELSKDLNGNHVVQKLLTKLRTEDSEFIFKECCNSTMEIATQRHGCCVLQRCFDNGSLXQRSQLCDKIMENINVLTTDPFGNYVVQYILTKEAEYGETKYTFKIVELLKGHAVRLSMHKFGSNVAEKLLRTPSVSSEIVTELVNSGNENIAKLLNDSYGNYVLQTSLDVAKNSDNAELYSTLANTLKPLLVGQIRSTPHGRKMISIIEGGNTIPSHGNARPGHGNRGGYHRNKNHRGNNQTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB56</accession>
    <name>A0A1E5RB56_9ASCO</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="2" type="ORF">AWRI3579_g2939</name>
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        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
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            <taxon>Hanseniaspora</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
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    <keyword evidence="1" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="19"/>
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    <feature type="chain" id="PRO_5009184678" evidence="1">
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            <begin position="20"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
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        <source>
            <dbReference type="EMBL" id="OEJ84122.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="78" mass="8466" checksum="347F4F382610C8CB" modified="2017-01-18" version="1">MRFSTLFASSLLLLKLSSALPVAQNTDNDDEYEYETVTMFVTNTVTSVPTKTVTVLDTTTVVQEVTMTPTAAFTPIVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB71</accession>
    <name>A0A1E5RB71_9ASCO</name>
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        <submittedName>
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            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
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                <person name="Lee D."/>
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            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="810"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="866"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="120"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="201"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="430"/>
            <end position="452"/>
        </location>
    </feature>
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        <location>
            <begin position="732"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1100"/>
            <end position="1157"/>
        </location>
    </feature>
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        <location>
            <begin position="1277"/>
            <end position="1374"/>
        </location>
    </feature>
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        <location>
            <begin position="1393"/>
            <end position="1451"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
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        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
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        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
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        <location>
            <begin position="145"/>
            <end position="175"/>
        </location>
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        <location>
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            <end position="247"/>
        </location>
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        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
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        <location>
            <begin position="283"/>
            <end position="294"/>
        </location>
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        <location>
            <begin position="1109"/>
            <end position="1130"/>
        </location>
    </feature>
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        <location>
            <begin position="1277"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1307"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1337"/>
            <end position="1353"/>
        </location>
    </feature>
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        <location>
            <begin position="1354"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1397"/>
            <end position="1434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1435"/>
            <end position="1446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ84149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1464" mass="158874" checksum="D534383C02CD4488" modified="2017-01-18" version="1">MEGHSDNTSGSHHNQQNRNQHHHHPKDNNNATTSSTNSNTGSHSTNTTTKDIIKKRFGRAVSTSSTASTSSTSTASTVNSANQISVPNAPLSLSPNHPLQKIPEININEKGELLYENVPLSDGSYHENSDGINSSDNPEYDVTIGSGDNNSTSSLGXLNNANLXQTPTSKTPPKTLGSYRNRAGKFSNTLSNLLPNISAKLHHSKKGNNKATAGESLNSKSSVNNKNSNDTPGSTGTSGTTNYNVSNLMNKVNNIRSTSNSSNPYIDSPSSKDRFSLNSTLQNSENGLESSSNSHLMGSVTPPELPLHPSTSATMQLAIPFPRSSLDSLSFAYDQSQGTXVLPGASSITGAGSVMGTRTRNNTINSQITSISGNYASNIWADNPQPFPQTLSSAINTSHNSGTKHSKSNTVNSAAQDSYFSFDYGNPAMNNTNTNLSKQQPSHLQASTSSWRARSKSNASSMYMDVTGQYYDQASTNEHPPSYISSKPTAXQDAGFYQNFTAGSYPTTVPPVVGDDIDPKSLNWVTTDVTAPPINHVANIPPTNTITISNIFSLQKQQTETLLSNAINLTSSSVATLCLSFGPVVSARTLSGLNLALVEFQSIDSAVRAKELLNNREISMVGIPSIVSFAKIIPVNVRSNSITPSLQSIKTANDHQMPQARSLLQEQLLNGSLSFQQNGNISIPMFTNGFEQSVDMNVSSNYLPIPGTNYXNNGSQSNLNFLAANKSHGYSFSSVSNNNHNNNNSTNNHSNSNGYNNAHGSNAIMEKEECPFKLPPPEMGQKIDELQQVISKFIENETNHNRKENIDEAQIQLLIKKSLNYKGTQKTNDFGPLPEPLQSKVFDSPTLRELRKMLDSHSLTDLEIEQLALCMLNELPELCSDYLGNTIVQKLFQNSSEIVKDIMLRKTNKYLTSMGVHKNGTWACQKMITMSTEHPSNKNLVVAGIQDFCAPLFNDQFGNYVIQCCLKFGFPWNSFIFESIIANFWTICQSRYGARAVRACLEATDIITKEQLIVLSAMIVVYSEYLSTNTNGTLLLTWYLDTCPLSHKQEILTPRLIGHVCELSCHKLASLTILKVLNHRNEFKAKRQIVETIFGGSPDFSKSRSFGSKSKESDXQEGESKEMQKSEESSAKSYQKSTPPLKKQSSEEATVDSKGVTKNSDECKTLINILSDTNYGPTFIYKVLSLSLLDSQVREYAIHQVKSLLMSSDPSLGKMIANAKAHRKLMEEIGLGSTSLSSSNNTSPIAHQANVHSQRHNSTDMLLMAPTASSQQDVNGAFSANASGKQNATKQPSLHPKVSSLSHIFGNSSSNVISSDPSVTSDEQQQFQQQHQQQPQHLATGQHLRNLSVGSVLSTKSTRSRGFSTGTSNFSNNTNXATMMPGGFYNFASASGTSNGHYSHPSTSSVVSPPVHISEMSGPSQTXALSSTQTXNSNDKNNXSQAGHSQNSTSMGSNYYNGYGSYMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBD0</accession>
    <name>A0A1E5RBD0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Altered inheritance of mitochondria protein 24, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3057</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the AIM24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBD0">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBD0"/>
    <dbReference type="InParanoid" id="A0A1E5RBD0"/>
    <dbReference type="OrthoDB" id="5295771at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007007">
        <property type="term" value="P:inner mitochondrial membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002838">
        <property type="entry name" value="AIM24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36959">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 24, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36959:SF2">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 24, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01987">
        <property type="entry name" value="AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ84204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="483" mass="54694" checksum="8753ED2D2EA97727" modified="2017-01-18" version="1">MTKSGALVSNPTRTLLKRNLSFTSRKQQHSHDNHHPAPTATTTTTIPADVDLKLFDNGFETPNKTQVQIMGNPPNMCKIKLPPSLPLYIKRNHLVSIFNIANLSKSVSLQNQWVHPWKNLLLWPNALPIYHKIITNPALSAKDTXGKQHFVDLLVSSKYNNCLTSLNLNGSKDWFIWGNFAKNIIAFEDNSSLTVQPEFKWSFGNSNFWKHLQSAQKLFKKYIHLSGRGITVLQSETGSSIYSINLKDNKDEVMIRLDNLLGCSGSNYTELISNMSPYLFTSTPEKSVSTETPSKEPKLKAGRNWNWNLPRFWERSNKKIEVTNNSVAVEPVVNEFSAKEFFSISKNILHKIISTVRYYYHQFINGNLSLSKNVLGKGLFSANYVTIRGPRTVLIQSNSIKSEILDKLMAPQKTSPPNVFKPVSPFDLNEAASKQKNLSQQMQESINNKNHLSYAYVDTQKNKVVFQSTPDFNASIEESVSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBJ4</accession>
    <name>A0A1E5RBJ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein MAK11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2467</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBJ4">
        <property type="interactions" value="623"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBJ4"/>
    <dbReference type="InParanoid" id="A0A1E5RBJ4"/>
    <dbReference type="OrthoDB" id="308449at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051959">
        <property type="entry name" value="PAK1-Kinase_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44675:SF1">
        <property type="entry name" value="P21-ACTIVATED PROTEIN KINASE-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44675">
        <property type="entry name" value="PAK1 INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="40"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="155"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="302"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="369"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="401"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ84265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="423" mass="48295" checksum="2B63A37406E2E4F4" modified="2017-01-18" version="1">MSSSRNQFRIIVGSYEHNILCVSLDLTNKNXPVFIPIFHFQAHSLSVKCLDSGKRYLVSGSNDEHIRIYDLQKRKELGTLLSHQGSITALKFSRSVAQQEPEESAQQTGDSXASVVKLSEKAKKRSADGKWLLSASDDQTITVWRVKDWEKFGVLKGHTKKINDLDIHSSNRIAISVSDDHSIRLWNLMSMKKSGVLKLRNYNQNGQFIRWLGDQHFVVALNDKVLIYEISSAKVIKEIEIENKKVIMHLEVCELDGEKYIVVGLNDGKVVFYDSNFLNNPSDENEGKESEEEDDVKPEFTLLGHSNRIKDFKFYSNSFGNYLVTVSSDGRIVVWDMASKEQVAVYDCAERLNCVTVIDENVEKFDSMKKRDQEEADVNNGEQSEVEADPQELKKIMLGKTKSKKNKKNNNNKRRKVEVEIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBX1</accession>
    <name>A0A1E5RBX1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methylated RNA-binding protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2686</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBX1"/>
    <dbReference type="InParanoid" id="A0A1E5RBX1"/>
    <dbReference type="OrthoDB" id="306690at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990247">
        <property type="term" value="F:N6-methyladenosine-containing RNA reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061157">
        <property type="term" value="P:mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.590.10">
        <property type="entry name" value="ph1033 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045168">
        <property type="entry name" value="YTH_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357:SF89">
        <property type="entry name" value="YTH DOMAIN-CONTAINING FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357">
        <property type="entry name" value="YTH YT521-B HOMOLOGY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YTH" evidence="1">
        <location>
            <begin position="160"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="301" mass="34115" checksum="59D9AD86A1F45166" modified="2017-01-18" version="1">MQADFDGAFSNASKQTESEKNCKDFLALTLKRLDTFINSSTENSANFSRGTEPVCEIGNCLDRNMKFQSKPYPTEKFIGNSNYWNLANGNYPNHNRALIGKLADPHPKEEYVNATLALTELQGFXDAPDSSVFNPMQKKTRLAVNYIKEFDIPACNDKNTKFFVIKSNSPQNILISQQHDIWASTKTGNARLSEAFENGKCNVILFFSVCGSGSFCGVSKMMSDIKSKKESKDLWNHKQIWTRDFEVKWLVSKVVPNRCFKHLSNPQNENKPVSQSRDTQVIPFHLGIAVWDIMETYALNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDJ1</accession>
    <name>A0A1E5RDJ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1796</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDJ1">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RDJ1"/>
    <dbReference type="OrthoDB" id="5559380at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051293">
        <property type="term" value="P:establishment of spindle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030473">
        <property type="term" value="P:nuclear migration along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013889">
        <property type="entry name" value="Karyogamy_KAR9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37271">
        <property type="entry name" value="KARYOGAMY PROTEIN KAR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37271:SF1">
        <property type="entry name" value="KARYOGAMY PROTEIN KAR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08580">
        <property type="entry name" value="KAR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ84972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="788" mass="90035" checksum="DF495627417EE87B" modified="2017-01-18" version="1">MTAQHGNGYHRAIKVKNILAETHSVSGYTDYASNRASSTKLSSPKLQAQDFRPPKLLNLSLSIQKLHTTLKESVDLIKDRNPENDSPTSLQIHYQEIVNFLQDMLENDHQFFEVILSQKSQLSMNAYCKWLNQGKTQFHAYFQSLSSIEEIILEILDKVEQLHVKNEISTDGDFFYSIEKCSEXYADLYNLCSFLKVHFNASSEFNEIYYDYMESINDEIMHMFESLNDLNDTNFTMSNNXPSLEELIRTLNSSVCFSKKYKRTMSGGSDTKNSGSNGSTGSSIKPGLVFVNAQDSLIYEHYKTLLNTFDPLTTSLTEILALKIEEFTATHKSADYLMELIDLIKIKYTELVGTYELLEAELKCLKNKLFFERWCQVFDFHFAGLDRIVSVALPSVLVAVKNFSFEKHPKIVIYYKTVITNHAQMVEKYFKFADLIMNKNFSHELTKRVTYKKNDLSFKWHTLKKDIPVDFADCFQKPNGFTKNLLLKDNSPSKHNVHSVRSLMNGTKKIENAIXNLSKSGVITQNGGFTNGRKLRNSRRLRRSLLSDVQSSKLSNTEADNLLTKFNRLSTLENIEEWKTLSKEFGRQSAGALIHQRLNIRPVVINDSDDLISPTKNDFSDFEEQLTDIESQLQADTNNEAIKEDGETSEEAYRAHVEKMKEVDKSLMKQASTSTLRISEEDRTLLGENFLLSPSTATFXSTRPSVSIFKTLKFKDALLHFSKQDTKINVPFDSGLAGLFAVQSPTSVGLNNSEYSFDQIIERLLKPEHQLXPLTNHTLRPLKLYKGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDN6</accession>
    <name>A0A1E5RDN6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Inner kinetochore subunit AME1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1785</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RDN6"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048743">
        <property type="entry name" value="AME1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20994">
        <property type="entry name" value="CENPU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inner kinetochore subunit AME1" evidence="2">
        <location>
            <begin position="244"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="327" mass="36891" checksum="87E71F501F1765B0" modified="2017-01-18" version="1">MSHEKFDIREFVHDDKVKKMKQRARGSFQNFDKLNEIQIDLAQLPLLRSNLELKEGXRNSLAESSVLGHTXLDDVVAHDREAALKETDSLKAHSVYAFQGQHSGNKDVYXLGGALDEMSSDTETASTVSNGSERSMFFGKKINEQIQXEPMDVADVADVSREFESNHSMGNSPEGYESHGFDKYESXPXERTXTEQTDLLDDAGSEMGTPEVSYLAQLFQINKTRSKTSVDLQTLADQFANPEXSQATVQFIDIIKEIIVEVMQEQFIGRSVELFENFSEEVGVLDRVSKNRDKMSIKLDCKIFERFLDLVVRDLDTLILLNGGEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDP3</accession>
    <name>A0A1E5RDP3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 15">Alpha-1,3/1,6-mannosyltransferase ALG2</fullName>
            <ecNumber evidence="5 15">2.4.1.132</ecNumber>
            <ecNumber evidence="4 15">2.4.1.257</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g1898</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 15">Mannosylates Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>a beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP-alpha-D-mannose = an alpha-D-Man-(1-&gt;3)-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29515"/>
            <dbReference type="Rhea" id="RHEA-COMP:19511"/>
            <dbReference type="Rhea" id="RHEA-COMP:19513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58472"/>
            <dbReference type="ChEBI" id="CHEBI:132510"/>
            <dbReference type="EC" id="2.4.1.132"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13 15">
            <dbReference type="Rhea" id="RHEA:29516"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>an alpha-D-Man-(1-&gt;3)-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP-alpha-D-mannose = an alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29519"/>
            <dbReference type="Rhea" id="RHEA-COMP:19513"/>
            <dbReference type="Rhea" id="RHEA-COMP:19515"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:132510"/>
            <dbReference type="ChEBI" id="CHEBI:132511"/>
            <dbReference type="EC" id="2.4.1.257"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14 15">
            <dbReference type="Rhea" id="RHEA:29520"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the glycosyltransferase group 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.132" evidence="5 15"/>
    <dbReference type="EC" id="2.4.1.257" evidence="4 15"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDP3">
        <property type="interactions" value="728"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDP3"/>
    <dbReference type="InParanoid" id="A0A1E5RDP3"/>
    <dbReference type="OrthoDB" id="448893at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004378">
        <property type="term" value="F:GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102704">
        <property type="term" value="F:GDP-Man:Man(2)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03805">
        <property type="entry name" value="GT4_ALG2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027054">
        <property type="entry name" value="ALG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45918">
        <property type="entry name" value="ALPHA-1,3/1,6-MANNOSYLTRANSFERASE ALG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45918:SF1">
        <property type="entry name" value="ALPHA-1,3_1,6-MANNOSYLTRANSFERASE ALG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 15 18" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="453"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="480"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="17">
        <location>
            <begin position="19"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="16">
        <location>
            <begin position="218"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="16">
        <location>
            <begin position="327"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="18">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ85001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="514" mass="58571" checksum="50BFA3E6CC6CD3BB" modified="2017-01-18" version="1">MAELLAPLEIAFMHPDLGIGGAERLVVDCALGLKSQGHSVTIYTSHCDMDHCFEEIKNGEIPVEVIGDFLPTNIGGRFAIVCATLRQLFLTFKLIRSKTIDNYDLFIVDQLSTSLPFIQAYKPRTLFYCHFPDQLLAKKGGLLKTVYRLPFNLIEQLSTGMADKIVVNSNFTKSVFQKTFRMLGEKLASVEVVYPCVDTDTXVNELMTSQDKKFFDTNFKSSVSDDTEFLLSVNRFERKKDIELAIKSFQKAQESLGSARKSKLKLIVAGGYDPRVAENSEYLIELEHLCDKLELNFETFHYGDIVAKSRTNSAKFVLQNKIDELXVVFMTSISSSLKNLLITKSRLLLYTPSFEHFGIVPIESMNLGTPVLACNNGGPMETVEQDKTGWLAEPNVEDWTKHILVCLLTTXKXQMKEQCQKRVLKYFSRIAMTESLEDKIGKMMQVPKKKKSNAPMVAMALLNLLVFYILQFLFLEKDHFYVYGIMMIFNTVFLKDRQWGFYWLAFAGLVHFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDT5</accession>
    <name>A0A1E5RDT5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Palmitoyltransferase PFA4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1817</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDT5">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDT5"/>
    <dbReference type="InParanoid" id="A0A1E5RDT5"/>
    <dbReference type="OrthoDB" id="331948at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="2">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="262" mass="31304" checksum="A16FE175B0AD92CB" modified="2017-01-18" version="1">MRFLFWVIVTTIYLLVLLVGRACHLYQIRSQPSYLIHTSEIVFLILLIPFDFFVFFTIFLLFARCARNQIFRGMTQIETWEWDRIESLYINKRLMPLMLDNAMKRFNIKRTRETDEQIHYLLKNQLKIPMDEYVNFPYDLDIYRNAKLFLGPWWSWLLPWGSPTMSDGTRFLQNELHEYDENADVXDKLLSLPWPPDGKKNTSLEDAASSSMQSLGNIYDSVEQELFKKRRTDPRLKLKRNEWYNDWGESLEDFGVEVSADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDV9</accession>
    <name>A0A1E5RDV9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Transcriptional regulatory protein SIN3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1816</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDV9">
        <property type="interactions" value="1154"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDV9"/>
    <dbReference type="InParanoid" id="A0A1E5RDV9"/>
    <dbReference type="OrthoDB" id="10265969at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000001">
        <property type="entry name" value="Paired amphipathic helix protein Sin3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000002">
        <property type="entry name" value="Paired amphipathic helix protein SIN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000003">
        <property type="entry name" value="Paired amphipathic helix SIN3-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1160.11">
        <property type="entry name" value="Paired amphipathic helix"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013194">
        <property type="entry name" value="HDAC_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003822">
        <property type="entry name" value="PAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036600">
        <property type="entry name" value="PAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039774">
        <property type="entry name" value="Sin3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031693">
        <property type="entry name" value="Sin3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346:SF0">
        <property type="entry name" value="SIN3A, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346">
        <property type="entry name" value="SIN3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02671">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08295">
        <property type="entry name" value="Sin3_corepress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16879">
        <property type="entry name" value="Sin3a_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00761">
        <property type="entry name" value="HDAC_interact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47762">
        <property type="entry name" value="PAH2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51477">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase interacting" evidence="10">
        <location>
            <begin position="862"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="76"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="172"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="459"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="630"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1079"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1528"/>
            <end position="1552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1652"/>
            <end position="1679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1712"/>
            <end position="1760"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="561"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="706"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="41"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="96"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="124"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="193"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="205"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="246"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="459"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="470"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="630"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1658"/>
            <end position="1677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1716"/>
            <end position="1732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1733"/>
            <end position="1744"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1760" mass="199698" checksum="0BFFCE47314B5D21" modified="2017-01-18" version="1">MSFSSNHSTNNSNANANXNSNTNVNAILNNTGTISNANMAPHHHSQHQHQRHHSASGGMHSRVQGXVVLPPASSLNLNRHESMDSNHSGGLGGSEGTATASTNNNMFYRLPSINDVGHSRKSSHGGSSNGNASMYNANVQLPSVSNTLMGGTSSALMAEDNKAKNMSIKSFNNNTSDSIDTSAHKKSISHNEATNATNATTNTTMNGISQSMSPTQNQHRVSFNSHLSSGHQSPTLPSINSIGHMQPSVSVHEHQQQQQFEQQPQQQQRANASSAHASPTNSVEAPSQENADADANTDNYRPLNVKDALSYLEQVKIQFNHRPNVYNSFLDIMKEFKAQTIDTPGVIERVSTLFAGYPSLIQGFNTFLPQGYKIEYSNNPNDPNPVKVITPYTGGGAVDLNTTAMHEKHYYEQQQQQLEQQQXLAQQQQLEQQRLEQQQFEQQRLEQQRLEQQXLDQQRLEQQRMEQQKIEFQQQQKTQANPAGNANGDVEFSQAINYVNKIKTRFQSQPEIYKKFLEILQTYQREQKPIREVYEQVTVLFQNAPDLLDDFKKFLPETPESHLQEQQYAEQQQQLQLQQQQLLLQQQQAYAEQQHYQEQQQQQQQQQQQQQQRELTYGKDVSTLNNNLSAGAQQQPQLPPLGSFSPPSKYQKEEFKQPAVAEHVENYQSYPQQEHIVTQGLTNDNIPVSNLREELSSDAQSSQLYLQQQQQQQQQQLAQQQLAQQQLAQQQQQQQQQITMYQQQQDTMVARPEIDLDPSLVPVVPEPLAPVEAEISLSYEATFFEKVKKFIGNKPSYXEFMKILNLYTQDLLTKSELVYKVEFYIGSNAELMTWFKQFVGFEELPKHIENIVHEKHKLDLDLCEAYGPSYKRLPKSDTFMPCSGRDEMCWEVLNDEWVGHPVWASEESGFIAHRKNQYEEALFKTEEERHEYDFYIEANLRTIQTLETIANRILNMSSEEKENFKLPPGLGHTSVTIYKKVIRKVYDKERGYEIIDALHEHPALTVPVVLKRLKQKDEEWRRMQREWNKVWREVEQKVYYKSLDHLGLTFKQSDKKLLTSKQLLAEISTIQLDQQSSLNNHSAAHHAHGNGGHNNSAKRSHPLTPKPKPQLDYFITDYEVLYDIVHLCRVCIYNATSYSYADKDKLLQVLVWFLKIFFGADYASIKASLXSKDTLVQDNKKVSAESAEQSKEDEVLPAGDGIINENGKRFREVEQDSGTVFDEDLSFKEIINKSKYKKLTNLDDLNEKEVSEHTKGTESDLDQEFFKNESQKPWIYGTLLDKINENGTVEDRTIFNAFTNTNMYIFFRFFIIAYERLVAMKKIDAQVTSDVNGRKDNEYVKDMTLSSAQLAEMGLDFKGDSAYRRLLELAEDLIDGKVEHQWFEESLRQAYRNKAFKLYTIDKVIQSIVKHAHTISSDNKTCHVMELFEKDRMSHNTSYKEQILYRLQVRNQMAPTEPMFRIGFDTTTXHVCIEYVPVDGTTVQHGSGTEEKWKNYVTSFALSHPTEGVSQDKVQNVVLEQVLAEDSRATEKTXAXGSGXXEKXSDENEQGDVDANLLEAKDSIKGVSYSDVALEINKGDYMLQTEFGSQDVFSRSSVNQYPRFSLTTAQPVNKNEXVKMMNSWLDASVKSHQSEPPVKSDXNXVHSDDAXLDEQNESEQQVLQDNPTQEPSSTSEPVASKXAVFSATAEIPSSQTNAASLSPEHIVSAAPVADSATANTSENLASSHADANSESTPFSPETTTQATENPEGSAGVXASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDZ1</accession>
    <name>A0A1E5RDZ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2224</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDZ1"/>
    <dbReference type="STRING" id="56408.A0A1E5RDZ1"/>
    <dbReference type="InParanoid" id="A0A1E5RDZ1"/>
    <dbReference type="OrthoDB" id="37297at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33875:SF2">
        <property type="entry name" value="ACR183CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33875">
        <property type="entry name" value="OS09G0542200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ85128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="213" mass="24453" checksum="D1E88E43B701C4B1" modified="2017-01-18" version="1">MLLSRTVMSLKPQFSKSHILTAVSSVQGTQAVPPNLVELYLDYNCRFSYKIFQKWDDAKLFDKETLSKYNLAFRFNHVIQPWHPFGTLVHELGIVAGSLKPELFWKYSMVLFHDSKELWTEEYTQSKTTIELYKELSEHCEKHTGLKADVVYDKLNGSGKDDTTKAIKYFTRYHRQNGVHMTPSIAVNGIILGNIESSTDTNKVLEIIQAQTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE06</accession>
    <name>A0A1E5RE06_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cell wall mannoprotein CIS3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2357</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PIR protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE06">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RE06"/>
    <dbReference type="OrthoDB" id="5415592at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005199">
        <property type="term" value="F:structural constituent of cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054508">
        <property type="entry name" value="PIR1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051153">
        <property type="entry name" value="Yeast_CWMannoprotein_PIR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254:SF1">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22799">
        <property type="entry name" value="PIR1-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184708" evidence="6">
        <location>
            <begin position="19"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Cell wall mannoprotein PIR1-like C-terminal" evidence="7">
        <location>
            <begin position="120"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ85114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="203" mass="21230" checksum="95FC0B8C59BF2869" modified="2017-01-18" version="1">MQFSKIAVGSTLIATSLAAGYTPGEPWSTLTPTATYTDGLSSFPSSFGIAVAPITTGSIYEKYAATATASATSGVTTTLAPXSSSSSTTATSTSXINTDPIQAAACLNDGTLKITLEDSILRDDKGRIGSIVSNRQFQFDGPPPQAGAIYAAGWSITQEGNLAIGDEDVFFQCLSGEFYNLYDEHIGSQCEPIYLQVLDLVEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REC8</accession>
    <name>A0A1E5REC8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mitochondrial intermembrane space cysteine motif-containing protein MIX17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1826</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REC8"/>
    <dbReference type="FunCoup" id="A0A1E5REC8">
        <property type="interactions" value="224"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REC8"/>
    <dbReference type="InParanoid" id="A0A1E5REC8"/>
    <dbReference type="OrthoDB" id="3973410at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055304">
        <property type="entry name" value="CHCHD2/10-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13523:SF2">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13523">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 2/NUR77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="162" mass="16646" checksum="D863980EE40F05C7" modified="2017-01-18" version="1">MARSRGSARSSARPMQSSRSAHTMAAPARAAPAQTPNYSHPQTAPAASAAPMQQVQQKQPGLFAQMASTAAGVAVGSAVGHTLGAGLTGMFSGSGSSEAAPVQADAQAQPQYMAAAPAPQQTACDVDARNFTRCLEDNQGNMQACDFYLQQLKACQQAASQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REF3</accession>
    <name>A0A1E5REF3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MINDY deubiquitinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1918</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REF3">
        <property type="interactions" value="295"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REF3"/>
    <dbReference type="InParanoid" id="A0A1E5REF3"/>
    <dbReference type="OrthoDB" id="10261212at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016807">
        <property type="term" value="F:cysteine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990380">
        <property type="term" value="F:K48-linked deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071108">
        <property type="term" value="P:protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007518">
        <property type="entry name" value="MINDY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033979">
        <property type="entry name" value="MINDY_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18063">
        <property type="entry name" value="NF-E2 INDUCIBLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18063:SF6">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04424">
        <property type="entry name" value="MINDY_DUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MINDY deubiquitinase" evidence="2">
        <location>
            <begin position="22"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="308"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="387"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="427" mass="48312" checksum="2F772E786516151C" modified="2017-01-18" version="1">MTTAQETVPTNGGQPAINSVVFETKLIATPQGTKQKILLQNENGPCSLIALTNLLVLSKLHKRYCLDLIDYVNSHETISLQDLVSVLCEAALHLHALEEESEGANPLDIHSLQQKLLSMNNGLMVDPKFDGNFRADASEDLFVFNIFQVSLVHGWCVEPVFDDPQSSILLKYSYEQAQQVLMEAYQLNSEANNVASTDPNYEARKYQVNKEAGYIKPFLARTVRQMTDYGLKHMNDILFDEDYVIFFRNDHFQTLYKQNGELFTLVSDAGYKKESQIVWQSLKSVNGSEDQFFDGNFELANPGDTFQAQQAQQSQQSQQSQKIQQSQQVQTTQPKQGSANXNDNGLTDEELAKILQEEEDAKYADNMQRRIAQKDRRQQSRRERNGQRNNANGNSNGGDSVDEFGNPVASKKSKKKFFKKKSKCIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REL1</accession>
    <name>A0A1E5REL1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Niemann-Pick type C-related protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g2439</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the patched family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REL1"/>
    <dbReference type="FunCoup" id="A0A1E5REL1">
        <property type="interactions" value="527"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REL1"/>
    <dbReference type="InParanoid" id="A0A1E5REL1"/>
    <dbReference type="OrthoDB" id="6510177at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015918">
        <property type="term" value="P:sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1640.10:FF:000029">
        <property type="entry name" value="Putative Patched sphingolipid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053958">
        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053956">
        <property type="entry name" value="NPC1_MLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032190">
        <property type="entry name" value="NPC1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45727">
        <property type="entry name" value="NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45727:SF2">
        <property type="entry name" value="NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22314">
        <property type="entry name" value="NPC1_MLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16414">
        <property type="entry name" value="NPC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12349">
        <property type="entry name" value="Sterol-sensing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184718" evidence="12">
        <location>
            <begin position="28"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="375"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="594"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="620"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="651"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="704"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="727"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="785"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1031"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1071"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1097"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1147"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1177"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="13">
        <location>
            <begin position="593"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ85314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1209" mass="136188" checksum="CBECB8EBA882A481" modified="2017-01-18" version="1">MLNLKPISSKAASFFWLTMFLSIFVSATSDKGLTNKARSQEHYPACAIYDQCGKKSLFGQELPCPKSSKFHPPKASAETLEIVSGLCGEEWLDQEYLCCTTEQAQKLEENIKKVENIIASCPACFKNFKNLFCSFTCSPTQRSFVNVTKTQLNYDKTKDVVTELDVFMNPNWASEFYESCKNVKFGATNGYAMDLIGGGAKNYSDFLKFLGDEKPLLGGSPFQMNYVYNVSETDGFKLYNETRALNCSDSDYKCACSDCFDSCPDIMDAPSSGQCKVGNIPCFSVGVVATYVCVFIGMVVWHVRMFKQKNKPSHLLATDSNENDGDNYPYAVRVENTTDSLFDNYSSLVYHKPSVGISKFFGKIACFCTVHCEKVLIFSASVIILLIISMFQFGKLELNPINLWVSPNSKKYQEKQFFDENFGPFYRAEQIFVVNETGPVLQDYQTLKWWFEVENYITGDLATNEDVGYNDLCFRPTEDFDCVTESYTQYFHGVLPGEESWESKLQMCADSPINCLPTFQQPLKANLLFSNDSFVLDSHAFVVTLLVDEHSARSIEWEKALEKYLLNLELPKGLRISFNTEMSLEKELAGNNDVWVVVASYLVMFLYSSWALNKSAQKSGVILGLCGILIVLSSITSAAGLLSALGLKSTLIIAEVIPFLILAIGVDNIFLIVNEYRAVDKTFSIEERIAFAVERISPSISMSFLSQFICFLLAGIVPMPAVRNFALYSATALLFNVIFQLTTFISILTIYEQYTSKIILPAEDENEDDNENYEKSKFSNFYHALLLKFRGMVLGISITISFAALFFIPSIELGLDQTLAVPQDSYLVQYFNDVYQYLNVGPPVYFVVQDVDVSTRDVQKRLCGKYTTCDEYSLSNVLEVEKNYTSITEPVANWLDDYFMFLNPSLDSCCRFKRGTSDVCPPRFPARMCETCFADDEWSYDMQGFPEGKDFMEYFDIWITTAPSDACPLGGEAPYSKSIKLNNDSHSIKTSYFRSAHSPLRSQDDYIQAYKDAEFISSQFEDLNMFAYSPIYIFFVQYASLVKLTLSLLAAALCALFIVSKVMIGSTRTSVILVLTVTMILIDIGGIMYFAGITLNAVSLVNLVICVGISVEFCIHIVRKFTFIDRDTEINNNARVLFAMKTVGKSVFWGITLTKIIGVSVLSFAKSKIFEVFYFRMWFALIIISSIHALVVLPVMLSMYGGKECYLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REQ1</accession>
    <name>A0A1E5REQ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Putative lipase ATG15</fullName>
            <ecNumber evidence="6">3.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Autophagy-related protein 15</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Putative lipase atg15</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">AWRI3579_g1702</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Lipase which is essential for lysis of subvacuolar cytoplasm to vacuole targeted bodies and intravacuolar autophagic bodies. Involved in the lysis of intravacuolar multivesicular body (MVB) vesicles. The intravacuolar membrane disintegration by ATG15 is critical to life span extension.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Binds to both phosphatidylinositol (PI) and phosphatidylinositol 3,5-bisphosphate (PIP2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endosome</location>
            <location evidence="3">Multivesicular body membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Prevacuolar compartment membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.3" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REQ1"/>
    <dbReference type="FunCoup" id="A0A1E5REQ1">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REQ1"/>
    <dbReference type="InParanoid" id="A0A1E5REQ1"/>
    <dbReference type="OrthoDB" id="58570at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032585">
        <property type="term" value="C:multivesicular body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005775">
        <property type="term" value="C:vacuolar lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034496">
        <property type="term" value="P:multivesicular body membrane disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046461">
        <property type="term" value="P:neutral lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006660">
        <property type="term" value="P:phosphatidylserine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050805">
        <property type="entry name" value="ATG15_Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47175">
        <property type="entry name" value="LIPASE ATG15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47175:SF2">
        <property type="entry name" value="LIPASE ATG15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0072">Autophagy</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="17 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="9 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="Fungal lipase-type" evidence="23">
        <location>
            <begin position="344"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="503"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="503"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00019241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00024663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00029828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="OEJ85382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="574" mass="64558" checksum="965A312C73565AD0" modified="2017-01-18" version="1">MIKLTNSNGKDIQDRNRNKLPRKFSHLKITLTGVLILCIVCIATYTSKSLGVFIPKKQNSKNTINGDHFLAEHSQTANNKGTFKLKHAFRHGVGEHHSLHQMYTFTDNLLYDENQLLSMQSKDPQQLQTAEEQQRFALLKQHAEMYPNHEIGLSTETIKLHRMKNRDPDFVTSYLDYSLEHPELSSKINIDWDFDQPVLAPNVSDKDTVLGLATMASNAYVRNPHEDQDWRNVSDPWKSHMSHGWDGSGVRGHVFANDNETLVVIALKGTSSNGIPGGGGETETIENDKINDNLLFSCCCARISYLWTTVCDCYKKSYTCDQRCLEKELRRKDRYFEATLDIYRNVTKLYPNADIWVTGHSLGGSLASFLGRTFGLPVVAFEAPGELLATKRLHLPMPPGLPTYQEGVYHFGHTADPIFVGTCNGASSSCSIGGYAMETRCHSGKVCIYDVVKDKGWRVNLLNHRIHTVIDEVLMKYDEVAKCVTPEPCIDCYNWRYTLEKDPKPDPKPKPDPSTKPKDSSSLSTSTIKITVTPTTVRTSKSKTHSSTQMTTTASSTCVGRNIFGWCTKYETLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REZ8</accession>
    <name>A0A1E5REZ8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1968</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5REZ8"/>
    <dbReference type="OrthoDB" id="3972462at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="361"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="2">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="384" mass="43181" checksum="2F3F7CA4E835FCF4" modified="2017-01-18" version="1">MQPPSTPTRPGXSSKTGXTSMATRQSGSANNTHLQQTPSRTQNSNYYNESGDPDHSLNSMLVTPQTVTKFKPTSAVLQNLSNTRTPEQNNNNQSGSSSGSXYSPEYETMDNSGSIFMDASIHTKGFNXGSLESEKPGLSYGQQQNQLHGITKRQHIGTLTPGHEEDARDLLMSKLLLRSPEYTPKTKPLRGLSLLQSHKSNENLKKSPVQGIKRKLAFEHIPNMHENDLLLEKLSRETXDIFESDDEKEETVGKDDLKPVFXDDQKQISKNLFQPMAVLEDEPLGFASPSTPSSKVISFEQAERWHNMSRLNSQGELRELSNDKEEQEVFITKKQLKNPFLSDKKKSXHRASLHSDKLEEVYSDSDSDSEVETEKLQTFIYKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF41</accession>
    <name>A0A1E5RF41_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SIR4-interacting protein SIF2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2164</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RF41">
        <property type="interactions" value="618"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF41"/>
    <dbReference type="InParanoid" id="A0A1E5RF41"/>
    <dbReference type="OrthoDB" id="1367865at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034967">
        <property type="term" value="C:Set3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045183">
        <property type="entry name" value="Ebi-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22846:SF2">
        <property type="entry name" value="F-BOX-LIKE_WD REPEAT-CONTAINING PROTEIN EBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22846">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="267"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="413"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="455"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="131"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="138"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="7">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="615" mass="68473" checksum="278553FF5DD1F37E" modified="2017-01-18" version="1">MSLTSEELNYLIYRYLQESGRELTALCLQEETRVLEFDEKFKQHIPFGCLVSLVQKGILYTEAELMVDYDGKVKTNAENVQKYQHHFTLAQALQIQGKKCREVKQIGRFALANDLEGEENEKNMKDLSRKLKEENEGTAETSQSNNENDGPVDKDAFTDAVAHDHSQRQQAPLSNENNKDIELTKSHSNSKSLKLETCFSFGPSTSCEFNNASLFAFAEKNSALGVTKIVQTVRETGTEEPKELVLQHNTXSDSTNLSLTEKIIGKSRSTLSTVTCLSWTATGDRIMTGTQSGELRIWTLDGTLNNVLNLHNSAIITIKWNLNGVFCLTMDVSNVVVVWNTFSGMPVQHFDLKEILNSYHHSEDKDESLGIDCCWIEADKFVIPGLNGAILLFDLDSTGATTGNQPMVPVGRLLGHTRTITCLDYDRESKMLLSSSDDCTVRVWRGTNSNFKNLFKGHTQPVVNACWFNASKCVISSSLDGTVKLWSSLPEFDNKEIGTSITDGVPIIYGSMSNSKTLYAVGRVDGAVLVYDLAELSQHFNDLVDACFPFDASKIADQFQQLELPIKAQYSLVSAGTSEENHIYITSMSWSNDDSMIGVSCDRGNSLILRLETLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RG81</accession>
    <name>A0A1E5RG81_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Mitochondrial metal transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g1867</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mitochondrial metal transporter involved in mitochondrial iron accumulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RG81"/>
    <dbReference type="FunCoup" id="A0A1E5RG81">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RG81"/>
    <dbReference type="InParanoid" id="A0A1E5RG81"/>
    <dbReference type="OrthoDB" id="435980at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000013">
        <property type="entry name" value="Cation efflux family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050291">
        <property type="entry name" value="CDF_Transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840:SF15">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0409">Iron storage</keyword>
    <keyword evidence="5" id="KW-0410">Iron transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="215"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="312"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="14">
        <location>
            <begin position="148"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="483"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ85583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="517" mass="57146" checksum="645C81F03AF90052" modified="2017-01-18" version="1">MMIIQSLRFNTRASLQLKVKVAGSLIVSTKKSILSPTPSRFCDHPSVFATQAFRKTFVTSRRLSKELTPEQIEQWKKDMAKLENQHIHMRESETEQNDSYKLGSMLTRPDPHEHHGHTHARTEESDTLMTGLLDKKEIKKNAGVRITWIGLLVNVGLALGKFAGGIVFHSQALIADSVHALSDLISDFLTLFSVKLAKKPANQDYPFGYGKVETVGALAVSSILTFAGVSIGWSSLLLVCGPYLPQMILEFMHIHSHAHAIPADIGAAWIAGGSIVVKEWLFQATKKIAEKDNSNVLLANAWHHRVDSLTSLVALVTISGGYFFGISSLDAIGGLMVSGLVIKAGYSGMKSACEELVDKAIPKSDAKYINIESVIKDSLDKMVSNNNAKKSYFLKDLNVMAAGPNLHAHALLVVPLQRWENVLGINEFEIVTDHLRTVVYKNIPHVKQLHIEYIEEKPELSSDEKKEIERQKKIGTAPVPEAALKSTESAHSHTHFGGLFGEENNNKNDAHSHHHHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGK3</accession>
    <name>A0A1E5RGK3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGK3"/>
    <dbReference type="InParanoid" id="A0A1E5RGK3"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="99" mass="11330" checksum="3FB7ACA7D4B7B6E9" modified="2017-01-18" version="1">MQSTNNNVSTGKSTMNSNNNNNNNNNNNNNNMSPQLNDVEACSPSLSAMLEYEAPQYYQWEEDEEDMIIDMNECSDLKPIQLKEYVAYTSMCQEFALKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGZ1</accession>
    <name>A0A1E5RGZ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional repressor OPI1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1306</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGZ1"/>
    <dbReference type="STRING" id="56408.A0A1E5RGZ1"/>
    <dbReference type="InParanoid" id="A0A1E5RGZ1"/>
    <dbReference type="OrthoDB" id="2441642at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013927">
        <property type="entry name" value="TF_Opi1_Ccg-8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38406">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR OPI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38406:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR OPI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08618">
        <property type="entry name" value="Opi1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="411"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="567"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="414"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="426"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="571"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="589" mass="65791" checksum="81D74286A14E522F" modified="2017-01-18" version="1">MVNLKEEDQENIKEKKNGVFIVDTISIKKEPTPTDEAHDFGKSKTTGNVVGNLKNETKTPEKMTQEKSIHEKGIQEKVPQAKTNSSGNSSDADEFVDSYSTNLITKKDIPQDVAIAIQALGDLKKSTKIINNIKKNANFVLENSKIHLNKNIQDIHTFKNITNKVVKKKFASRRKSTKKGQWSLDNGKSGKDEEGKESYDDNDEDDAANNEEDHTEEYYSGEEDTMEDTQEDTLEDTREDQRLDGLTKDCSIPKLRKKSKRRRISQAIKSHKLNLSIESKKKLFTCLHLLKLANKQLSNKVTGLQILVQHEERNMSEKKIGNKNMSEADVSGASGNALIGGTNIHSANSSSAMIPNSMYTPSSSAKNEIVGTVKKVYTLISKYTGNSLPEPARSKVRESLLKLPTKLQTYMSNDIEDDDEDDDKVDDSAKDDDKVDDSAKEANYLLKNDPKNDPNSLQIKQEDAEKLGLWEGPASKQRTHRVLKKVSKNKKMLLLAKESLEMVSSVMDVVDETLGKAEEWVKHKQEIKELLRKNLQQGITKNPAKMIGSSMNEVKVAALQIKTITGSAAESASTDDNNNDNITANNIHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH21</accession>
    <name>A0A1E5RH21_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Thiol-specific monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1055</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FMO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH21">
        <property type="interactions" value="629"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH21"/>
    <dbReference type="InParanoid" id="A0A1E5RH21"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00370">
        <property type="entry name" value="FMOXYGENASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ86201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="449" mass="51128" checksum="AA74AD6C0AD354ED" modified="2017-01-18" version="1">MTKKLAIIGLGPGGLGSLKVLLDSLDGPESRLRDNIHIDVFEALSDIGGLWNYNASISPKAPHATMYDNLETNLHKKLMQYTDFXFKNVKGNFPTRFEVLEYLXSYYKEKILAKHAQKFEVGIVTLHFDXKIIAIERNEEENTWSLTSGKGKXFAEYDDLIVSTGHFKQPYXPKKIPGYEDWDKHLPNSLLHSKNYQNPKLFKDKTVVVVGGSSSGSDILIQITSTAKVPVIWSVYNPEKNXFPVNPDFTKKDVGVITNLDYQTRSATFANGETVKDIDYILFATGFKYDFNEFKPNALPPSFKLEKEGKVIKGLYKHLVVADNPTVFFTVLPKNIIPFKLAEVQTSFIIAVIEGRIPQELIKADDETSLEESLHAFETPANMEYSNSLYDLVKKYYPALFQSFRPTYWENDIYDHRFRAVAEKFARQETLNKLAVDSRANNTSYVIPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH61</accession>
    <name>A0A1E5RH61_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-like modifier-activating enzyme ATG7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 10">ATG12-activating enzyme E1 ATG7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Autophagy-related protein 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Ubiquitin-like modifier-activating enzyme atg7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1061</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Preautophagosomal structure</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATG7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH61">
        <property type="interactions" value="765"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH61"/>
    <dbReference type="InParanoid" id="A0A1E5RH61"/>
    <dbReference type="OrthoDB" id="338614at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019778">
        <property type="term" value="F:Atg12 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019779">
        <property type="term" value="F:Atg8 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032446">
        <property type="term" value="P:protein modification by small protein conjugation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000243">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme ATG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.70">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme ATG7 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032197">
        <property type="entry name" value="Atg7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042522">
        <property type="entry name" value="Atg7_N_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF3">
        <property type="entry name" value="UBIQUITIN-LIKE MODIFIER-ACTIVATING ENZYME ATG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16420">
        <property type="entry name" value="ATG7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ubiquitin-like modifier-activating enzyme Atg7 N-terminal" evidence="12">
        <location>
            <begin position="11"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="11">
        <location>
            <begin position="278"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="13">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ86241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="605" mass="67964" checksum="92C30561666086CD" modified="2017-01-18" version="1">MESRGKLHFASPFQVFVEPSFYSELRDLKLHKLKLTTXEIPIQCSVNNDGQATLAQTSFDVAXKSTKAENLCLKGLVRNFNTLEGFKDLNKPEHLQTFSKKIFEENTDLDDLCSNVYXISFAELKKYTLLYWIAFPVFQFEGTVFKISNLQHPSDSNDLKDCLGKLIGKYVYKDSDCLLVRQFGKFNGNLPIYVKSVISKXYYEAKQTKFCIKLVSQTQLTXMDLDLKVSGPEISISDLRVTGWSRNSSGKLSPKFIDLXSMLDPLKMSEQSVDLNLQLMKWRIQPDLDLDVIRSQKVLLLGSGTLGCYIARALLAWGVRNITFVDNGKVSHSNPVRQPLFTFDDVGKNKAQAAMENLKKVLPAVSATFHVLEIPMIGHPIVDEAVQRKQYFELEQLIKAHDVIFLLMDSRETRWLPTVIGKANGKLVMNAALGFDSYLVMRHGDSVNGLGCYFCNDIVAPADSLKDRTLDQMCTVTRPGCALMAASLAVEIMVSFLQTNRNNNVNCLGDPTPHQIRGFLSDFTNVRLETPQYNHCSACSSPVVSAYLQDGWAFVKTAIDNPKIVEDLSGLTKVQEDAEKRLAELDLSDFNDFSDDDGEENFVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH88</accession>
    <name>A0A1E5RH88_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Peptide transporter PTR2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1211</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RH88"/>
    <dbReference type="FunCoup" id="A0A1E5RH88">
        <property type="interactions" value="1105"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RH88"/>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006857">
        <property type="term" value="P:oligopeptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018456">
        <property type="entry name" value="PTR2_symporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01023">
        <property type="entry name" value="PTR2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="303"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="399"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="475"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="519"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="555"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="586"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="687" mass="77420" checksum="523C9C1BF54DE913" modified="2017-01-18" version="1">MSNSFTEKNENGITHINSDVDIDNKFADEKNKMQNIKLHEKSVDLDTTDGSLNKEPTSGDSNLVYETTLYEEDDEDFVDFRKPTPEEEKTLRRTTGNIKWYLYFLLLVEFAERASYYGVSDRINNFVELPLPDGSKSGKVYTAGEDPGALNMSLSRANSTLNALSFFAYCFPLVTSYFCDMYFKRMTMLKAGVFIGILAHILFIIAAVPSVIAQPNASYGVLFAGIFVLSWCCACIKPCLLPTILEQYPHQENVVTKTKKGEKVILDREQTLNKITNWFYFSINLGSITDIGIGYSARNCGYWVAFLIPLCIFMILPFILWPLESKIYDPKPAGTSVMNDIFKLLKVAFEGNFIKRIKNKTFWEYPKPSYMQEAQPDRVGWRKNKPGWYPERVVNDTKVTLSACVFFLYYVMFNINDMGLGALLNNLSASMTTNGVPNDVINNFNPLAIIFFVPFLDLAFYPLCRKVGINWTPKWKIFTGFMTAALAMMVGAILQSWVYKHSPCGDYASSCDQHAPITLWYTAILYALTGLSECFAMTSAYAIAFLRAPNSMKTFVMALFLFTTALSSIMGIIISPAMVDPLLVRVFIGITVVGAAFAFGFLFQYWNLDETMAEEMAQQKMLDLAQKQQEEADLLIEQNDAINSTSYELNEETDGLNALASRNTHRGNIKDLENLSPIVSAEVAAKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHK0</accession>
    <name>A0A1E5RHK0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Covalently-linked cell wall protein 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1271</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHK0"/>
    <dbReference type="InParanoid" id="A0A1E5RHK0"/>
    <dbReference type="OrthoDB" id="3973514at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008427">
        <property type="entry name" value="Extracellular_membr_CFEM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05730">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00747">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52012">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184746" evidence="7">
        <location>
            <begin position="22"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="CFEM" evidence="8">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="149"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS52012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="280" mass="26658" checksum="247013E0471AFBCF" modified="2017-01-18" version="1">MRASVLTXVLSALFLSQFASAGNPACLLACVAQVSKKSSSCTDGMQDVSCFCENESSSIQSCLNSICPSGDSGNAWTGFQGVCKSFNAXVASSSSSSSASSSASSSASSSASSSASSSASSSASSSASSSASSSASSSASSSASSSASSSASASSSASTTPASTTSSSSTQEEASSTSSSVPTSTEESSTAAPSSSTAEDFSSVSSTVVSSTSVSASSVNEDVSTTFASSSASSLSTVVSSVSSSANSTTVPTKSSEDAGIKLSGNAVTGLLGVLAALFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHL0</accession>
    <name>A0A1E5RHL0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TECPR1-like DysF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1096</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHL0"/>
    <dbReference type="OrthoDB" id="74314at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052816">
        <property type="entry name" value="Peroxisomal_Membrane_PEX28-32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010482">
        <property type="entry name" value="TECPR1-like_DysF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28304:SF1">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28304">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06398">
        <property type="entry name" value="Pex24p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="TECPR1-like DysF" evidence="7">
        <location>
            <begin position="25"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="482" mass="55032" checksum="93868D8C5FC52193" modified="2017-01-18" version="1">MSTIRTTKAELTPKSVVRDLSYLGTKFAQFNSLQDSIAXIFQWENYAKSLLFLVIASFTCFRPIMLLIIPLIYLNVYILIPYYCVPQYTAKYQQEQNQKNPIVLMKSHSTYKESLVDIVLTKLLKPVPQTVTVDPLSADTGVISSGDSAKSGKALYHTPEEVPNSMEFVINMKDLQTMQGKFVNFMRDTEKFLYKNEKLXNDKAGAIMTSQILAVLTIVLYFIRTLINWSLLTMIVLWSALFWNHPLSNALKSFFRNPKKLKMVDLGSESETESLVDTDESLELSTSGLDTATTTSADLQPTTLTAESMAEPTTKDLTTATNTPSTGNKFRSFTRNVTKVIRSAYPIEKDWVQVYEIQYKDHANNSWIPAVFTLSPTEIYLNSLENNKQHSIKSVESLDLILPPKNWMYTPESTWQHINGGKEANFMVNWLLDLNIPLSDLDLWKIQKSSNSSNPLEKGFIYFKHNKNMRIRKLYRPIVFIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHM0</accession>
    <name>A0A1E5RHM0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1013</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHM0"/>
    <dbReference type="FunCoup" id="A0A1E5RHM0">
        <property type="interactions" value="731"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHM0"/>
    <dbReference type="InParanoid" id="A0A1E5RHM0"/>
    <dbReference type="OrthoDB" id="3972831at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18086">
        <property type="entry name" value="HsC9orf114-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003750">
        <property type="entry name" value="Put_MeTrfase-C9orf114-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12150">
        <property type="entry name" value="CLASS IV SAM-BINDING METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12150:SF13">
        <property type="entry name" value="METHYLTRANSFERASE C9ORF114-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02598">
        <property type="entry name" value="Methyltrn_RNA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="413" mass="47339" checksum="A75BACFF6DAD20CF" modified="2017-01-18" version="1">MAPSTKKSPPNGKNLKNKKQKNNTKKSKTTQQSKPKISNNKKANVPVKVKKDNLINRKEKNIKVSTKDVNYSICIPTSILNNCSNLEQITYTLYQIAKSCTLYNINEVVILKDTVESQTSAKSSKLKENRLSKDMIIATILQFFVTPPYLVKSVFNKKYWNYFKYCEKLPRLTSLPFMRHVNDSDPANDQARYREGLSIRMEHPNKNQSSGSSKKKEAYQQTKYINVGKSEVLELKNQLIPTNVRVTCDLVEKKVVSPEEAYGNYFGMKSSYGYHIRVVDRFSQVFMNAVTNYSQTLWINSGDFYFNKESNTYNNKVVTKIPVLAKIIKATDSEEQSKMEDSAEEVPQILLMFGKWKHLATHFKANSAKDFDDSVQLHDFFDAELQLPKAVPMGNIRTEDAVMIAMTMLDTLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHW1</accession>
    <name>A0A1E5RHW1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">37S ribosomal protein MRP17, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1381</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHW1">
        <property type="interactions" value="250"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHW1"/>
    <dbReference type="InParanoid" id="A0A1E5RHW1"/>
    <dbReference type="OrthoDB" id="10259681at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15465">
        <property type="entry name" value="bS6_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000529">
        <property type="entry name" value="Ribosomal_bS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035980">
        <property type="entry name" value="Ribosomal_bS6_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribsomal_bS6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00166">
        <property type="entry name" value="S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21011">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21011:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS6M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01250">
        <property type="entry name" value="Ribosomal_S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54995">
        <property type="entry name" value="Ribosomal protein S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="131" mass="14978" checksum="B007772A3E17E448" modified="2017-01-18" version="1">MLYELVALVRTPRVLTKQKYEEAKEVAFTAGKLVLENRGVIRRVIPMGQSFLPKXVKKNQEEHFESYNFLMLFDASAGVQTEILRSLRKDPRVIRSNIMKVDTSKTYDVTSSIDMAQGKSSLLDIIKDKTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHY5</accession>
    <name>A0A1E5RHY5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1411</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHY5"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ86496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="285" mass="33114" checksum="C4A74A7DD277E321" modified="2017-01-18" version="1">MLHLLPYEVSXNIIAEISKQPYDTENGMXGNTEWNVLNIRLVSTHWDQLFLYGIERKXGSISCTDXSCPGSWKEYYQHFILKKFIPLYGXDVANHRSSLNIHDTFKGAFSACWENMQGTVNARLAIYFEDLKKISRSQLLLHKLQVKLNGMCLFVPELLSYXLLGEKSMHDCSFKNQIVIVDLGPVKKASEYTMELKMPFSGNESNDTSLEFLFLEFGEALSTNEWLWCNFHIDHLNNYLEHRNISKHITYQFRYGNXQKNQTNVPERNESIFCTPKRDTLXSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNA4</accession>
    <name>A0A1E5RNA4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative mannosyltransferase YUR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g981</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNA4"/>
    <dbReference type="FunCoup" id="A0A1E5RNA4">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNA4"/>
    <dbReference type="InParanoid" id="A0A1E5RNA4"/>
    <dbReference type="OrthoDB" id="439943at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000051">
        <property type="entry name" value="Alpha-1,2-mannosyltransferase (Ktr4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF10">
        <property type="entry name" value="MANNOSYLTRANSFERASE KTR2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ88365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="529" mass="62333" checksum="DD2A77BB7DA86EA3" modified="2017-01-18" version="1">MKAIVVRGVLLLFGIICFFALNYSRVLSNPTNSSSSKSLQFQVRSNEIKDRWITERRSRKISAHDVKPSSNTYVDGFDETRYMNEQGDQSLVFQMGSRVPVDWKPKFNDSYLDFLHKGHLDIKEFNNFNSELDDWLFYVTAYHFQIKLPEVLTLFGRPDSPYFSYTVYKPPFVENRNNTIAKSCILMLVRNIELEGALQTIRRLEDRFNKDHKYDWVFLNDEPFTLEFITATSAMCSSSKIKYGMIPRELWSVVPDHIDMEEANRRMESMEAQGVLYGGSLSYRHMCRFQSMSFYKHPAVLEYRYYMRTEPDVDYFCDFNEYDPFKLMELKRKKYGFVISMYEYEDTIPTLWESVEEFIEETALKEGTDEFAVGDGFQFLTDDQLIDRGNANNMRSAQKVVDTLNGDYNLCHFWSNFEVGDMDGFYRTSRYNSLMDYLNLEKPGFYYERWGDAPVHTIAAMLFLNKTEIIHFDTLGYFHAPFGTCPLSENTRLQQKCLCDPQNEYNIDIEPHSCLMKWWKNGDGKRFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNF1</accession>
    <name>A0A1E5RNF1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphatidate phosphatase APP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g683</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNF1"/>
    <dbReference type="FunCoup" id="A0A1E5RNF1">
        <property type="interactions" value="77"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNF1"/>
    <dbReference type="InParanoid" id="A0A1E5RNF1"/>
    <dbReference type="OrthoDB" id="3972932at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019236">
        <property type="entry name" value="APP1_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052935">
        <property type="entry name" value="Mg2+_PAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28208">
        <property type="entry name" value="PHOSPHATIDATE PHOSPHATASE APP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28208:SF3">
        <property type="entry name" value="PHOSPHATIDATE PHOSPHATASE APP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09949">
        <property type="entry name" value="APP1_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatidate phosphatase APP1 catalytic" evidence="2">
        <location>
            <begin position="450"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="674"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="717"/>
            <end position="729"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="842" mass="95746" checksum="005815198AF73906" modified="2017-01-18" version="1">MYRKELFISKVIGFHKVISKGQRPLGKHHQLQSLYKQQNLPLFQTNINPCEAKNILVASHQIRSLSLKNFVNIVFTRLVKKYKYKLAKRNLTKQIKRQQKLEKTQQFETYLNSNKAIPRNPLSRKRTLFKKLFRSKPPLPAPLNNKASIMESNVSKRQRLFNMVKTTKDVYIPAVGSSLSNLKNGTSTSLKNYYDRNNNFAYSNKNLPYDLYGASDGSTVPEPLGTSNIKIITYSTYTHYNVYLKKYSTTIKGAVVMDGVMNRKNRLMYSLCKRMIRDPQVEHKAQAEDEIKRLTELDDLDSVATASTPASEKLLRHSESNTSTPLQNENFSSDEDSSSPSSFSPATSPKRYMSKSDFLSGNAVFNERISAFFSKGLPYINMDITVDGQKITDPIKTNAKGFFTYALVTDTTPRNVVFTISDSQPAGAFPHGSTKINSEVQIINYNQNGFAVISDVDDTIKHTGILENKRSIITNTFVHDDDQWSIPSMSTWYNNLKQTAKCDFFYVSNSPIQLYAPLKKFIFKHYPPGVIFLKEYASGGLINNLMSSSSNRKLETIKHIVSSFPQKKFLLIGDSGEHDLEAYLEVCKRFPSQTLAVYIRCSKGSMTDNNLNGESSIIQKFNNYIEKNCWKMERTKQEILPGSPYSVSPAKNLQAKAAPPLPPRPRAHVSFSNLEKEKNDNQTSIADTARQDGKFVNNSAIKTRTPPPVPPKRRELRSRPVERISRDNSHSSLGPAFSVYKNQEKQCFENVNASSAKIDSTAQELTSAADTVDDAYYTPSSQNDYGAYDQYFDKRMDNWRNRVMDACSTLNNIHQSEGFKIQFKFFQQDCLEESQRLLEKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNT5</accession>
    <name>A0A1E5RNT5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Protease KEX1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AWRI3579_g579</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S8 family. Furin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNT5"/>
    <dbReference type="FunCoup" id="A0A1E5RNT5">
        <property type="interactions" value="167"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNT5"/>
    <dbReference type="InParanoid" id="A0A1E5RNT5"/>
    <dbReference type="OrthoDB" id="300641at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007323">
        <property type="term" value="P:peptide pheromone maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04059">
        <property type="entry name" value="Peptidases_S8_Protein_convertases_Kexins_Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000026">
        <property type="entry name" value="proprotein convertase subtilisin/kexin type 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000005">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034182">
        <property type="entry name" value="Kexin/furin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002884">
        <property type="entry name" value="P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884:SF14">
        <property type="entry name" value="NEUROENDOCRINE CONVERTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01483">
        <property type="entry name" value="P_proprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51829">
        <property type="entry name" value="P_HOMO_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 14 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 14 15" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14 15" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 18" id="KW-0732">Signal</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184847" evidence="18">
        <location>
            <begin position="24"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="681"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="domain" description="P/Homo B" evidence="19">
        <location>
            <begin position="464"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="602"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="730"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="613"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="742"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="773"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="797"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="13 14">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="13 14">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="13 14">
        <location>
            <position position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OEJ88536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="816" mass="90531" checksum="D48CD6F50F6A1E8C" modified="2017-01-18" version="1">MSLFSLLCGLMVVSIFKASVANASLHAVPPKDHSKNRYFAVESILSPEELTNMHPDWNFEHAVRGFEDHYVFSTNNTHNSLQKRNELQLDNNTTFYEELPLKRLHKRRPVPREQTEEQAAAVKLFQDTIQKYDIKDPLFNRQWHLINTYSPGNDVNAVSVWDKGITGAGVTVAIVDDGLDYENPDLADNFSPEGSWDYNDNTNLPKPRLADDYHGTRCAGEIAAVRNEYCGMGVAYNAKVAGIRILSGELTAEDEAASLIYGFQTNDIYSCSWGPPDNGIDMDGPSDLVKKALTKGVQKGRNNKGSVYVFASGNGAMYGDNCNFDGYTNSIYSITVGAIDYKGLHAPYSESCSAVLTVTYSSGSGEYMHTTDINNQCSDKHGGTSAAAPLAAGIYALVLEANPSLTWRDVQYLTIVSGVEVNADNEKSDWQTAHNKFGKRYSHMYGFGKLDANAIVELAQSWENVNEQVLHEFPKIEVNEIAKTVDDTIESVLEVTEEDMREFNIKHLEHVTVTVNADSNVRGLNHLELISPNGTVSKLAVERKIDKKKSGYDNWTFMSVANWGEEGVGQWKLRAKITRDVNELTLKDWTLKIFGEARDEQIGHSDETNGDDTTTVATSTVSEVEPTTFVEEPTSTIAEVSSVTASSSPSSTTNDSPKPSKTGTAEDSYKSHLIDTAENHYFIFLIIFGLILIVGYYILFRNSRRKFQRLRAEAYEFDIIDSDSEYNASVNTSQQSIPAEGGEPDLEDYDFDLSEEEDITGQAHLEEHEPAPESSNDKNSNSQEQETAPESSENKTNDATGQDNPFESPNDPETKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNX2</accession>
    <name>A0A1E5RNX2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N(6)-L-threonylcarbamoyladenine synthase</fullName>
            <ecNumber evidence="1">2.3.1.234</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g914</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-threonylcarbamoyladenylate + adenosine(37) in tRNA = N(6)-L-threonylcarbamoyladenosine(37) in tRNA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNX2"/>
    <dbReference type="FunCoup" id="A0A1E5RNX2">
        <property type="interactions" value="420"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNX2"/>
    <dbReference type="InParanoid" id="A0A1E5RNX2"/>
    <dbReference type="OrthoDB" id="10259622at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:tRNA N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072670">
        <property type="term" value="P:mitochondrial tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF6">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Gcp-like" evidence="7">
        <location>
            <begin position="55"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ88584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="393" mass="44194" checksum="96B2467B327CDD60" modified="2017-01-18" version="1">MYVRRTLQKYASFSIKSCTYNFLRRYNVLAIETSCDDTCVSIVNTEGEILVDLKKSLKTVEEDGGIIPTKAHIHHQVEIGDLARKALEDANVSLSKDIEFIACTRGPGMPGSLGIGYDFAKGLSVASGKPLIGVHHMLGHLLMPTMFDSSITFPNYSMLCSGGHTVLVYSENLYTHNIIVDTLDIAIGDSLDKCGRELGLKSNMIGKEMEKYINDEFIAKGRVDDAQEINQLTKKYPITEPLKYNLKQHPKFVSFSFSGYLSNLKKIIQDNDKVDKPGLCYLIQEHHFKHLINKINDARKYKMLPRENVPLILSGGVGSNLRLRAKLEHEVSSIFSSFHFPPVRMCTDNAVMIGWAGIKLYDTLKLKTNYNTSPIRKWPLDELLTVDGWAKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP39</accession>
    <name>A0A1E5RP39_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g675</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the OST3/OST6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP39"/>
    <dbReference type="FunCoup" id="A0A1E5RP39">
        <property type="interactions" value="272"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP39"/>
    <dbReference type="InParanoid" id="A0A1E5RP39"/>
    <dbReference type="OrthoDB" id="67566at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021149">
        <property type="entry name" value="OligosaccharylTrfase_OST3/OST6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12692">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12692:SF0">
        <property type="entry name" value="GH11935P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04756">
        <property type="entry name" value="OST3_OST6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184826" evidence="10">
        <location>
            <begin position="20"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ88639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="352" mass="40319" checksum="F0C1A8287ED22D22" modified="2017-01-18" version="1">MKLLHFLQLLCCFYLATLGQCLSNERLLKEAKKGRNNVIKLTDKNYKRVLQGARDSYLVVFLTATQDSIGCSLCQAMEPEYDILAESWFKTHPKGENTLKNDGDKKGLFFAKADFSAGDNHEVFQHFQAQQVPMMVVFSPTQDTSLQTINTFQQINIPAQAQPGDPRIQMLINSFQHYFELENYVIYKPVNYGSKIVTGLSCLASIFLLRKFKTIVSQIVFSKTLWGLTSCLFITVMCNGYMFNKIRKTPYAGNGGSNGQVQYFQPGMQTQYAIETQIVTFLYGTLGLLTLFLIKFVPKISNYYENKKQFYNSALSYMLTAGVLLFLIFFFSSGLMFVFRMKYPQYPFKLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV58</accession>
    <name>A0A1E5RV58_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Aconitate hydratase, mitochondrial</fullName>
            <shortName evidence="14">Aconitase</shortName>
            <ecNumber evidence="14">4.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g508</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>citrate = D-threo-isocitrate</text>
            <dbReference type="Rhea" id="RHEA:10336"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="EC" id="4.2.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="14">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.-" evidence="14"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RV58">
        <property type="interactions" value="924"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV58"/>
    <dbReference type="InParanoid" id="A0A1E5RV58"/>
    <dbReference type="OrthoDB" id="2224430at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01584">
        <property type="entry name" value="AcnA_Mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000002">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000003">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000004">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1060.10:FF:000001">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1060.10">
        <property type="entry name" value="Aconitase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050926">
        <property type="entry name" value="Aconitase/IPM_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015932">
        <property type="entry name" value="Aconitase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006248">
        <property type="entry name" value="Aconitase_mito-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01340">
        <property type="entry name" value="aconitase_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005558">
        <property type="entry name" value="PRK07229.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43160">
        <property type="entry name" value="ACONITATE HYDRATASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43160:SF3">
        <property type="entry name" value="ACONITATE HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9 14" id="KW-0408">Iron</keyword>
    <keyword evidence="10 14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="16">
        <location>
            <begin position="74"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="17">
        <location>
            <begin position="590"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="530"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ90821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="787" mass="86202" checksum="77C182D92448D6A5" modified="2017-01-18" version="1">MLASRNLFTKARTTATRGLASSSASASASASAITRDSKVHQNLLEDHSFINYKQNLENVNIVRERLNRPLTYAEKILYGHLDKPHEQEITRGVSYLKLRPDRVACQDATAQMAILQFMSAGLPQVAKPTTVHCDHLIQAQIGGEKDLTRAINLNKEVYDFLSSATSKYNMGFWGPGSGIIHQIILENYAFPGALMIGTDSHTPNAGGLGQLAIGVGGADAVDVMSNLAWELKAPKIMGVKLTGKMNGWTAPKDIILKLAGMTTVKGGTGKIVEYFGDGVETFSCTGMATICNMGAEIGATTSVFPYNKSMIXYLNATGRNKIADFANQYHKDLLIADKNSEYDEIYEIDLNTLEPYINGPFTPDLATPISKMKEVAVKNDWPLDVKVSLIGSCTNSSYEDMSRAASIVKDAASHGLKAKTIYTVTPGSEQIRATIARDGQLETFKEFGGIVLANACGPCIGQWDRKDIKKGDKNTIVSSYNRNFTSRNDGNPSTHAFVASPEITTAFAIAGDLRFNPLTDKLKDSEGKEFMLKPPHGIGLPPNGYDKGENTYQAPPKDRSSVQVQVSPSSDRLQLLKPFKKWDGKDAFNMPILIKSLGKTTTDHISMAGPWLKYRGHLQNISNNYMIGAINAENKKANCIKNELTNTYGTVPEVAGYYRDHGVRWVVIGDENFGEGSSREHAALEPRYLGGFAIITRSFARIHETNLKKQGLLPLNFKNPKDYEKINPQDHVDVLGLTTFAPGKDLTLRVHPADGSAPWDAALTHTFNEEQIEWFKAGSALNKLAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVC2</accession>
    <name>A0A1E5RVC2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNA polymerase I-specific transcription initiation factor RRN5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g304</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVC2"/>
    <dbReference type="STRING" id="56408.A0A1E5RVC2"/>
    <dbReference type="InParanoid" id="A0A1E5RVC2"/>
    <dbReference type="OrthoDB" id="2240312at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000500">
        <property type="term" value="C:RNA polymerase I upstream activating factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000182">
        <property type="term" value="F:rDNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001181">
        <property type="term" value="F:RNA polymerase I general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039601">
        <property type="entry name" value="Rrn5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28079">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28079:SF1">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="483"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="490"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="536"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="631" mass="71308" checksum="9CC18B2892BE61D1" modified="2017-01-18" version="1">MKNNRITKRKHDAQLFDNNDCVIKYISLFNDEVKNALFAPAFKNDLQTSNIHLDSVIAQLETSNFNAGDFTDEENANYTNFLADKNVGSGGGGNGGGGGSCSDSDSLSSDTEEEDLESEDHSVQYSFENDTTILSTNEEIGIPWSDVEKQVFFHCLSRYSIHLVDEWALQIPSKTKYEIVMYYDILRNNYDSMRNKAMNRSLIKRADLPIAYEMDQEWCEIEETLAQCVSTALFLGNDSGSNESDNTDNNDGDSDSDNNNRLFELDQKWQERWLQIYSKHGLMEYYPVSKNCLPLDNVDTLQYLESCVKSFCSHLIKETILNMDAYEDDFEVVKNPVNMDFTTNGDMVVLQRGSFAEKSGKPRLLLTVTVADIHKAIVKMKQNTRKYGHHPYTLFEQVYDTLKKYNIRLDSARKNAQLKIDMNYTKEGQDESNENKNFPSKEYITDETVDKDYSLLKYKKSNIGQIPALVHKTINDQISLDSLSGNVKNTTTETSTTSKETSTTPKETSTISSATPKETSTISSATSKKTSNAATDTFTNNTKSNITLPQYNIPQRFQSLASPNDMSNSNSNSNITNIGFTPGSGDFPRSAEYKDLLKEQKLDHNDITKSQNYQHALLAYFASDYVDNTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVI5</accession>
    <name>A0A1E5RVI5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g565</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVI5"/>
    <dbReference type="InParanoid" id="A0A1E5RVI5"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="68" mass="7328" checksum="AEB754CA5C0FFDF7" modified="2017-01-18" version="1">MTLDTKRINLNSPSARDPKNSSFHAKSHVTGIASTQTKQAALDQLNVAPARQLPTGSEHHCIEHASNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVK7</accession>
    <name>A0A1E5RVK7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA polymerase epsilon subunit D</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">DNA polymerase II subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g377</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVK7"/>
    <dbReference type="FunCoup" id="A0A1E5RVK7">
        <property type="interactions" value="234"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVK7"/>
    <dbReference type="InParanoid" id="A0A1E5RVK7"/>
    <dbReference type="OrthoDB" id="1707486at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008623">
        <property type="term" value="C:CHRAC"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008622">
        <property type="term" value="C:epsilon DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22928">
        <property type="entry name" value="HFD_POLE3_DPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051377">
        <property type="entry name" value="DNA_Pol-Epsilon_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46172">
        <property type="entry name" value="DNA POLYMERASE EPSILON SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46172:SF1">
        <property type="entry name" value="DNA POLYMERASE EPSILON SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="7">
        <location>
            <begin position="13"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="112"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ90980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="173" mass="19254" checksum="69E3A19103D43831" modified="2017-01-18" version="1">MTQEKISINDLLFPKTTVINIAKNNETDTRKIIMTKDTQTALQRASTVFLNYLMNAARETAYNNNKKSVNVDDVFDSVEKLGFGVGFSDQLRALFQDYQDASAKKKEVREKLKSLNSGSVNGEKAVAEEKVDGESDIEDKEDTQVNTNEVNDENVNETVQNEGAGGLKKPKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVU3</accession>
    <name>A0A1E5RVU3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative magnesium-dependent phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g491</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVU3"/>
    <dbReference type="FunCoup" id="A0A1E5RVU3">
        <property type="interactions" value="178"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVU3"/>
    <dbReference type="InParanoid" id="A0A1E5RVU3"/>
    <dbReference type="OrthoDB" id="2865258at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010033">
        <property type="entry name" value="HAD_SF_ppase_IIIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010036">
        <property type="entry name" value="MDP_1_eu_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01681">
        <property type="entry name" value="HAD-SF-IIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01685">
        <property type="entry name" value="MDP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17901:SF14">
        <property type="entry name" value="MAGNESIUM-DEPENDENT PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17901">
        <property type="entry name" value="MAGNESIUM-DEPENDENT PHOSPHATASE 1 MDP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12689">
        <property type="entry name" value="Acid_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01131">
        <property type="entry name" value="C1.5.2:_MDP_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ90949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="178" mass="20757" checksum="41FD44DA165FB11B" modified="2017-01-18" version="1">MGYPDIAVFDLDYTIWPCYCDTHINPPFSPVKSSNGEIRTLVDSHGFEISPFKDVFKILLDLKKNNVKLLSASRTWAPDIAIAMLKKFIVVDPETNEKHTMFELFDDCQWGEMSKVNHIRNGLKEIYPSKSQTLQDYSIVLYDDESRNKDVEKYGVKFMYLRDPEKGVTWEIYQKSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVY3</accession>
    <name>A0A1E5RVY3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g300</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 81 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVY3">
        <property type="interactions" value="215"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVY3"/>
    <dbReference type="InParanoid" id="A0A1E5RVY3"/>
    <dbReference type="OrthoDB" id="4473401at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052861">
        <property type="term" value="F:endo-1,3(4)-beta-glucanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1170:FF:000001">
        <property type="entry name" value="Endo-1,3-beta-glucanase Engl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1170">
        <property type="entry name" value="glycoside hydrolase family 81 endo-[beta] glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005200">
        <property type="entry name" value="Endo-beta-glucanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040720">
        <property type="entry name" value="GH81_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040451">
        <property type="entry name" value="GH81_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983">
        <property type="entry name" value="ENDO-1,3(4)-BETA-GLUCANASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983:SF0">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-D-GLUCOSIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17652">
        <property type="entry name" value="Glyco_hydro81C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03639">
        <property type="entry name" value="Glyco_hydro_81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52008">
        <property type="entry name" value="GH81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 81 N-terminal" evidence="9">
        <location>
            <begin position="83"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 81 C-terminal" evidence="10">
        <location>
            <begin position="410"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ90893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="769" mass="86667" checksum="EF3767599232EEB6" modified="2017-01-18" version="1">MGYDRPAFPIPNKIRDKFKLPDLHKLSISSPFFTKNEPESFAKPPSADVSKSANYENSLFTQPISTNEPLPCFPRSTHEVSKPLNMQNTENKAVETNKFYGNMFVESQTCPVWTHPYSLWWCKEPGLSGMAVSYIEDKQKVFGPGDPPQYYFSPTNIKSFVFASTSFQSADDMELGFENPKHMSIQSSILLKSDKNKKITFPLVQGMGFVTAIYSGLNPKLQSAVGIRCFENLGKINGSKITKFSILLENEVKWSLYVFLDGSKVQPNMEVDIDLKQIDGQTLVPVTDLSSLPLVFQLIPSSHDCVDTIVGNYLVDCKLEGEVSNSDTGQYSFNYLVQGLHPQDGALIFCMPHHVQTMTDNTKTTQLSPPITLDSTVYGKLTGFKTSFLDFQVETPGSNYCLTHIESKSFSQAQINSIGLAATNEIQNGNVEAESNLDSMYFSGKALAKYAWILYTTHYILHDXNLTVQLLEKLKSAFQRFVLNKQILPLNYDTTWGGLVSSGTSTQDFGNSYYNDHHFHYGYHVLTGAIIAQIDYEISGGNDTWLQDNKNWINNLVRDFANPSEKDPYFPVFRSFDWFGGHSWAKGLFASGDGKDQESSSEDANAAYALKLWGQVSKNTNLEKIANLKLGIMRNSFNNYFLYSTGNTVEPNQFIKNRVAGIFFENKIDHATYFGSNIEYIQMIHAIPINPISTFIRSPQFVKEEFEEKLKPVVDNITDGWKGIIMLNVALYDPQHSYQFFNLPDFQRQYLDGGQSLTWSLAFAASMTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZ90</accession>
    <name>A0A1E5RZ90_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Vacuolar protein-sorting-associated protein 46</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g11</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZ90"/>
    <dbReference type="FunCoup" id="A0A1E5RZ90">
        <property type="interactions" value="693"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZ90"/>
    <dbReference type="InParanoid" id="A0A1E5RZ90"/>
    <dbReference type="OrthoDB" id="10266568at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10476">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="210" mass="24050" checksum="F6CC5C6F923A81CF" modified="2017-01-18" version="1">MSSRANSLENTLFQLKFTSKQLLKQAQKSLKEAKVNEKKLTKLLSQADSSDNAEIVKIYASNVVRKKNEHLQLLKLQSKIDGIISRLNTSLIMKNLNSNFYKITKNLDVALRTMNLEQITMIMEKFESQFEEIDTNVESFNSLTNSNVMMKQQDNDEVDNLISKLRDENGLKLPDVNNTEEVPISIDEPTTNKEDELEDKLAQRLKALRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZH9</accession>
    <name>A0A1E5RZH9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nucleoporin NUP188</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g268</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Nup188 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000002">
        <property type="protein sequence ID" value="OEJ92148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZH9">
        <property type="interactions" value="176"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZH9"/>
    <dbReference type="InParanoid" id="A0A1E5RZH9"/>
    <dbReference type="OrthoDB" id="102511at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044611">
        <property type="term" value="C:nuclear pore inner ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018864">
        <property type="entry name" value="Nucleoporin_Nup188_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044840">
        <property type="entry name" value="Nup188"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041634">
        <property type="entry name" value="Nup188_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048883">
        <property type="entry name" value="Nup188_N-subdom_III"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31431:SF1">
        <property type="entry name" value="NUCLEOPORIN NUP188"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31431">
        <property type="entry name" value="NUCLEOPORIN NUP188 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18378">
        <property type="entry name" value="Nup188_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10487">
        <property type="entry name" value="Nup188_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21093">
        <property type="entry name" value="Nup188_N-subdom_III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup188 N-terminal" evidence="10">
        <location>
            <begin position="180"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin Nup188 N-terminal subdomain III" evidence="12">
        <location>
            <begin position="416"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin Nup188 N-terminal subdomain III" evidence="12">
        <location>
            <begin position="638"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="domain" description="Nuclear pore protein Nup188 C-terminal" evidence="11">
        <location>
            <begin position="1028"/>
            <end position="1341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ92148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1368" mass="157056" checksum="407AD1AB968E2951" modified="2017-01-18" version="1">MEKLSFSSVYEDLKNSNANYNNQSAKIENNPLIKXFISEKSQFLHQPVRFYTATSHSDAKIKNLSKKHNLNADEVARIVKQVDERANDGCYXEEIENEKKYFAFFLKYLASDTKYLSLLMKHKQTILYDCLELLQNSVETESLLQTITALVINSKQQNSDFVIKWLNYVYDXRDCILPITVINSLLLLGLNTAEATIDVFENQETAKEITEFLKTHNLECPLVYYFWTFVLYISDGEKEKKDIEYFIRKSDMVLEYMEQFELDTFYSTILSSFWKVALHFVTFDAAICKSIAKTLQKCSVQTVEDFLIYPEFETKLEVLRQKLPLFDEALMPLLYLASVNADFANYELRSCNTFATKVKLGELDYDLLDDEANIIVLKSEFLISPPLDSSVLLPIPQDTRAKYISTSTSDDEVLLILLYNFNGWSLLGRLLKSFASENLVALINMLXFNIDTPLERATEVLQQLSAFIEEDDIVAVIFEIFEKSLQVKNINVASSIVDFIKALLKNFPHLVWLHLVKSQLLDKNGTLLSMTSTELMYGDFKFLLSLNDLIDGLRNDAIKVETEVPAKTKEDIITKLLEYQINVFESYQFWKFEEPKDRFKLGSSLCKSFMRAQTYFSQTTISSRFSTSLINSILSAKSVEGDEVFKDFIESHMTLARNFVSFSEQIGAELINHSNDLAQIYMRFHEYKIPALSLMTAMVECTREEQLSILSHLEKGSSFVNALKYDLQYPVSNFAYMDHFYQFFGALLRNGQSGLCILFLTGEKASKSTQLKPSDNSLFFILKDQLLQDLPDEVFINLLKVVSLTLNIWNDKHNVFSQDAKVLDKLVQILQDFKPTDDFRKYDLIANVTETCAFLYYTTKHSAIDNLFSNENIPTIVKTIFDVEDAVPEXESVTKSRFAQLFPEMPFTYDIPYLDKTLADNQEWLEFKPQLQKLVDGLKLRASKDHAAKTWGALLTSYVKNTGSPLNNNFLDLTLIFLQNDSSTLLAQRLELTFYIFYSFFKTKKNIPDDTLILIFTLLQSIFKNIEFPDSIHSSNIRPYYKTCLRLVLLTMNMVSDGNKFVETLYNELLKFMYSVIGTGTTLTLDKLLANVNKENEPYFVSEKIQDLLALLAIFNKIREMQPSESFKLALSNALSESGMLKSIINMYSNSHLPRNDPFILADVSLNFIVELCRMETVAEKLIANGLYAVLLESPSSLSIQKGGLTANTDAELYNLWLNGVLSVVLQLLGMFGSKVISETCLFVRYFEKQIKFCIHSWANYKNICVSTLSIQETGQIILLYKIMVMLEYDQYVEKVVLPGFHNENAKTELFTSFRQLLTHPKFLNSRLIATNESEQKILDSPESRSLFVKQVTADIKQLQNSIFSAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZV2</accession>
    <name>A0A1E5RZV2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Eukaryotic translation initiation factor 5A</fullName>
            <shortName evidence="9">eIF-5A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g190</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Translation factor that promotes translation elongation and termination, particularly upon ribosome stalling at specific amino acid sequence contexts. Binds between the exit (E) and peptidyl (P) site of the ribosome and promotes rescue of stalled ribosome: specifically required for efficient translation of polyproline-containing peptides as well as other motifs that stall the ribosome. Acts as ribosome quality control (RQC) cofactor by joining the RQC complex to facilitate peptidyl transfer during CAT tailing step.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. Binds to 80S ribosomes. Actively translating ribosomes show mutually exclusive binding of eIF5a (HYP2 or ANB1) and EFT1/eEF2. Interacts with DYS1 and LIA1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="9">eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-5A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZV2"/>
    <dbReference type="FunCoup" id="A0A1E5RZV2">
        <property type="interactions" value="1021"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZV2"/>
    <dbReference type="InParanoid" id="A0A1E5RZV2"/>
    <dbReference type="OrthoDB" id="9975114at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045901">
        <property type="term" value="P:positive regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045905">
        <property type="term" value="P:positive regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006452">
        <property type="term" value="P:translational frameshifting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04468">
        <property type="entry name" value="S1_eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000007">
        <property type="entry name" value="Eukaryotic translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000034">
        <property type="entry name" value="Eukaryotic translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001884">
        <property type="entry name" value="IF5A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048670">
        <property type="entry name" value="IF5A-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020189">
        <property type="entry name" value="IF5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019769">
        <property type="entry name" value="Trans_elong_IF5A_hypusine_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00037">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11673">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 5A FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01287">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21485">
        <property type="entry name" value="IF5A-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003025">
        <property type="entry name" value="eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01376">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00302">
        <property type="entry name" value="IF5A_HYPUSINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="7 9" id="KW-0385">Hypusine</keyword>
    <keyword evidence="11" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Translation initiation factor 5A C-terminal" evidence="10">
        <location>
            <begin position="84"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00064934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ92366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="157" mass="17221" checksum="48391C8384A6536F" modified="2017-01-18" version="1">MAEEEHTFETADAGTSTTYPLQCSALRKNGFVVIKSRPCKIIDMSTSKTGKHGHAKVHLVATDIFTGKKLEDLSPSTHNMEVPVVKREEYQLLDIDDGFLSLMTMDGETKDDVRAPEGELGDNMQAAFDEGRDINVTIISAMGEEAAISFKDAPKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZY9</accession>
    <name>A0A1E5RZY9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g154</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZY9"/>
    <dbReference type="InParanoid" id="A0A1E5RZY9"/>
    <dbReference type="OrthoDB" id="5596576at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055011">
        <property type="entry name" value="Tag1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055010">
        <property type="entry name" value="Tag1_M"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22786">
        <property type="entry name" value="Tag1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22787">
        <property type="entry name" value="Tag1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tag1 middle barrel-like" evidence="2">
        <location>
            <begin position="28"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Tag1 C-terminal" evidence="1">
        <location>
            <begin position="217"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ92406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="347" mass="39471" checksum="0B3697F9C65495D1" modified="2017-01-18" version="1">MINEQFAESFGEHVLVSNINTSLTNHFLHKFSWQPVFPDCNGNLDIIFPSTKTFFQVTLVERTDAKEDDFKALRVFGSIYDDGFPFEFTNGVCYADDINTITPMNLFLSKVFNSTELLRLGVVETSFLSLYDQPHQNDVPGELEQNPSHIKNILNPGLTPLPIDLNFTLNSSEFLREVGIDKKTFKIIKDPNNRRNPNSYKVQGEGFAEIFIPYVTDLKILVEKLKGKTKIIDQISNKHFLTVTMDVWEDCKVEYIYDDEEDITFGATLHKDHMSQPVHMKLIFQLDDAEVEVVDNAVLGRVMSRTFWQGESPVLIDAVMDLIVKNDVLGEFMLSDIPGAGSTVITR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S061</accession>
    <name>A0A1E5S061_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g193</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5S061"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ92363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="590" mass="68386" checksum="AA49C5AAAA9CCB3A" modified="2017-01-18" version="1">MALSSKNVIDLHFIADHDDQQLMDMHFXKIIETIFFQHNLNWFVVKNYILFDKLLDDLYDLNGIQDCLCGHEDDDDKDIDVLENXKYVSVNYHPKGNRISASRTRDCSDAHVQCDIVYKKLHALLIFFEKIITRILTPNSSSKDTSSSATHKEHIQMRTLLTKQDETATSNTKTESSLSFLPNHLFTNHEFLWSFISLVPVVQDLKIKNPGNDKEVVTLSCDEGLVLFHVNHKLLKKDFVNFKYFTNSSTSSLPFTVLSYTNEYDKMQMCAYNDAVAKDLQIELSEDVHKNTEEAAIYNCYKLRSTETEMIKLIKKIDSKILFAKFDFANNDSKLLRVDDVDTDLLQQLLYVWPTCYTIATTESLXARNKVLPKNKVYIDYYVLPQDLNSQQKRLKRFKQKFSQVKHYKDIKTGALQGNNACATDAVFVKNINVSPGKVTKPHSQSATSSPHKRKTVPTRMNHVLASKSSIFKTRENSTHAELLQKVKAKEEYNRLNFAKNQQEIQENCNRMFENNLQKIEKILFVLDKTRPYSINKLMDLITDSLSNMEMLSTEEIERILMELHKRNVVECVDYNGLKIFNIIDRVKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S0K2</accession>
    <name>A0A1E5S0K2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g114</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5S0K2"/>
    <dbReference type="STRING" id="56408.A0A1E5S0K2"/>
    <dbReference type="InParanoid" id="A0A1E5S0K2"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="493" mass="55150" checksum="30B54BAE17DF595B" modified="2017-01-18" version="1">MPFPINFRTFSNKANLPISPISLTNVSAASVKNNEIAQTPDGKEAQATDPKAKVSKNAQLKKALQKICRKLCSKKDRKKELPTDLTMGTQRFVTNDPTMTLRGPSSRTGPSAPLDIFALFALPVSPDTDDATSVEITETAPQPRVSRAKKAKRVCCKKIKTLFNFKKRLSSNCSVSNIQALPESAAASFHSEEVEEKEYVAAEEECVSADEECVSADEECVSANDTQQLRSILKTTSKYDQPSTPVLPERESMNIALEPSVEALLISYENALSSLEAYELSLVTSISRKEQPLDWRSLRTPVPQDSSSRTPKTVPEWKLVHLKAQYLLCHLNRQAAMVACRDAQDSKASPEHVRYLGNKWLATRLPFLQSVVNLNAKTDELETLQPGLVNDFDPHAYFNSMEKPAQLFSTIINESVLLPFSVKQMEQRVDLDYRNEPKPLSDHVFANEKYRLMLAELNYFKFLLQKKCSSCLDLDLRGLKCFPNLVSWGIVSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5QZZ1</accession>
    <name>A0A1E5QZZ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional activator ARO80</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4648</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000017">
        <property type="protein sequence ID" value="OEJ80228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5QZZ1">
        <property type="interactions" value="349"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5QZZ1"/>
    <dbReference type="InParanoid" id="A0A1E5QZZ1"/>
    <dbReference type="OrthoDB" id="2262349at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009074">
        <property type="term" value="P:aromatic amino acid family catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052780">
        <property type="entry name" value="AAA_Catabolism_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31644">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR ARO80-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31644:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR ARO80-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="31"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="732"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="747"/>
            <end position="779"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="900" mass="103153" checksum="1BE0231A0DA00CAA" modified="2017-01-18" version="1">MYSVLKKACEPAKETSNDMPSLKTYRKAYQACLACRQMKQKCVQDVEVDGKTLDKCYRCTRKRIPCVFVARKSHQKKILKNVLKADNTDKSFATEVDVENLMRNNEXSPSKLSQEKAKNETSQVSTTHGALNFLAQAAGKVSQQQEEQQPQKPLMSLPADKHKKTLKDMEYIPFLLSEYEARKFVELFFITLHPFYPFIPIHFQDCDELCKYPLLLCAILTISSRYHTFEEIYRGQDHGPENNTTHIEVHEKLWTYCQMLFSQTIWAEASTRSIGTVFAFLLFTEWNPRAIHWKCTDYATGGLGVMNFQGSGYGNNDKSAQSMRSTGNGLAPMRRSDRLGWNLSGAAIRLAQDMNFIDTSCKIFLASHISEINIALSMNKMGLLRTSFANIDLKRTVRDQMEXSVDPETFVHCADSVNCGECHRWDYWKSKFYTGNVQYDSLFLKEFXEDDKSLYRIDPEDTEYLSNKKVKNDKLQFSAPQLAKLEILKIMSLGYDSIYNDKMVKYIKDAVYNLNTLSVLHELMESWYMRYAPLFAKKCNSNGCIDLAKMSDGKYVMEVTRLIDAENLCMDYYYCQLYIYSLSFEVVIIHSGKSIIETDTTELDAGIKYLELAFNAANEIIGCGKRLYSVGMLRYAPVRLVSRIVRSIAFIVKCFMTFTNKKSSLNSSRYGNPNFNNRFLFCYGLDKIVQMIKDASFLMKETSPDEIHLGTKYSAILEFLYKKLDKTVTDMNQSENTEDKTVFSNDNKNNSNNNNNNNNNKSNNNNKNNKNNKNNNNNNIIGGESNSNGTNESFKEPLFTNEYNNAATFNNTDDDLLTWFDSINAEYATATNTFDNSVNFNDATLRDGNINMNNNKNQTTNTNTINNTNNTNNTNNTNSNDYIGLNFVDAWTEMINEHIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0E7</accession>
    <name>A0A1E5R0E7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">DNA repair protein RAD57</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g4501</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0E7">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0E7"/>
    <dbReference type="InParanoid" id="A0A1E5R0E7"/>
    <dbReference type="OrthoDB" id="1861185at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000730">
        <property type="term" value="P:DNA recombinase assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042148">
        <property type="term" value="P:DNA strand invasion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061982">
        <property type="term" value="P:meiosis I cell cycle process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19491">
        <property type="entry name" value="XRCC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047348">
        <property type="entry name" value="XRCC3-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942:SF66">
        <property type="entry name" value="RE19845P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942">
        <property type="entry name" value="RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="8">
        <location>
            <begin position="83"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="363"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="363"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="381"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="511" mass="58191" checksum="13664069264DD32A" modified="2017-01-18" version="1">MDLYDELPSSTLVYDENFEQLLDSARVHKISXLTILTLTSKELVPLLKRSINEISLFKDILEKEYDEYLXTKLQNKKXNVKQQLQAFTTGEPNIDEMLGGGIFTENITEVYGESSTGKTQFLMQLSLTVQLPVEKGGLGGKCVYISTEGDLFTNRVQQLIDTRFSDQETQEESSGLPXMHKVNQNNIYYVNCSDFETQEHILNVQLPVLLEKDPDIRLVIIDSISHHIRAEHESHSFLESQNNRFKIERLSANLLKLCSEKNLSIVVANQVSNKVFNFQQYENTIHGSXSAENKLSLKKNYTECEKYDYQLGYMVGWKASNIKYTHMLINFNNTVTDEVLSDDEEYAYLDNLMCQLTSGSVVKRETNNDSNKLHGRERDNQSNNSDCTSSSASNNGTSKPQVENLLKRPNSNKAPYLYKKRKYSHKNPVDTAIPTLGLTWANNVTTRLLLKKKMVVAPKLKLSDKSQLSLEDLRPEKALVKKRTMKVVFSSFCQANALMEYTITPKGIVCX</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0G4</accession>
    <name>A0A1E5R0G4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA-directed RNA polymerase II subunit RPB3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g4557</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0G4"/>
    <dbReference type="FunCoup" id="A0A1E5R0G4">
        <property type="interactions" value="1045"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0G4"/>
    <dbReference type="InParanoid" id="A0A1E5R0G4"/>
    <dbReference type="OrthoDB" id="270173at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07031">
        <property type="entry name" value="RNAP_II_RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.120.12:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="entry name" value="DNA-directed RNA polymerase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00320">
        <property type="entry name" value="RNApol_arch_Rpo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001514">
        <property type="entry name" value="DNA-dir_RNA_pol_30-40kDasu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022842">
        <property type="entry name" value="RNAP_Rpo3/Rpb3/RPAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050518">
        <property type="entry name" value="Rpo3/RPB3_RNA_Pol_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001988">
        <property type="entry name" value="PRK00783.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800:SF2">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="Insert subdomain of RNA polymerase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00446">
        <property type="entry name" value="RNA_POL_D_30KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RpoA/D/Rpb3-type" evidence="9">
        <location>
            <begin position="18"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="290"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="290"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="302"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00072506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="313" mass="35067" checksum="14507460C481C62D" modified="2017-01-18" version="1">MNLNTEPQLKIREATSENVDFILSNVDLALANSLRRVMIAEIPTLAIDTVEIDSNTSVLADEFLSHRLGLIPLVSTDIDDLAYNRDCFCEDYCDKCSVILELNAIGESESTTNVYARDLQVVSDLRGKNIGKPVILQKDGNGPLICKLRKGQELRMRCIAKKGIFKEHAKWGAASAIEFEYDPWNKLKHTDYWYEQNAKEEWPKSKNCDFEDEPNDDEPFDYNAEPNNFYMNVETVGALTPDQVVLRGIRVLQDKVNGIALELKKMDQEKFNAADGGMSVYGGADAFNTGDNNNNNAGGFSEPEDDGYGNEAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0I6</accession>
    <name>A0A1E5R0I6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mediator of RNA polymerase II transcription subunit 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4538</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0I6"/>
    <dbReference type="InParanoid" id="A0A1E5R0I6"/>
    <dbReference type="OrthoDB" id="203279at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.160">
        <property type="entry name" value="Mediator of RNA polymerase II transcription subunit 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009332">
        <property type="entry name" value="Med22"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06179">
        <property type="entry name" value="Med22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="29"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="31"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="60"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="174" mass="19482" checksum="D8A8BF4BC55E64FE" modified="2017-01-18" version="1">MSEITRINKIVDQLVSSLESIVQYSQFSYKDEDDNNNGDTNDEGDDSDHGGLGNDDYFGSTDQYGNPQSTNTKSSAFEEITTISVNDSTQSISLVHTKAMQLITGIQELLLITRSFKERWVIGQASTFLKFAENGEALVQEEKSTQESENDEMLDSFEQKSQLLSKAMDELLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0J2</accession>
    <name>A0A1E5R0J2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g4527</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0J2"/>
    <dbReference type="InParanoid" id="A0A1E5R0J2"/>
    <dbReference type="OrthoDB" id="5985073at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002889">
        <property type="entry name" value="WSC_carb-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01822">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00321">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51212">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="3">
        <location>
            <begin position="5"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="109"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="320"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="377"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="464"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="4">
        <location>
            <position position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="473" mass="49963" checksum="4A17E6475303D453" modified="2017-01-18" version="1">MSDTSYTFENCYSSLPSSFTFSNSYTYQTPGWCSQQCVGSKYFALFNHGDCYCCDDDVDLSSLSTSDQCTAYCYGFNEQMCGGEQDAYDIYAYNTALTGGSSSIATTTLSSSTSSTSTTSSSTSTTSSSSSTSFTTSSSTSSSTSSSTSSSTSSSTSSSTSSTGDDVVTSFVTSKVLYSTGLQTHGGSTVYVTQVVSTVISTATATANASKNDGNSASTGSSSKKKVPVGPIVGGVVGGVCGLVLIIICLIFIFKKINKKREEERLEREYQEAIKPRPFLGKSVSAFMGFDARDKMGDNSDDESSDDSGNTTAAFTPITNNDNSNSNNNNNNNNDNNNNNNNSNNDNRTTGSXNGIARTQSEYDVDKEFADMNFDHTGSSGNTSTTANTNNNKSSGQGHNNNNNNNNSNTNNNPFDNDAYVIQSNMSSNSEQSHNGMHVSNPEGVQSGDNENDDTGSIVEGYTQKLTVVNPDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1C9</accession>
    <name>A0A1E5R1C9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Heat shock protein, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4071</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the GroES chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1C9"/>
    <dbReference type="FunCoup" id="A0A1E5R1C9">
        <property type="interactions" value="799"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1C9"/>
    <dbReference type="InParanoid" id="A0A1E5R1C9"/>
    <dbReference type="OrthoDB" id="184876at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00320">
        <property type="entry name" value="cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.33.40:FF:000002">
        <property type="entry name" value="10 kDa chaperonin, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.33.40">
        <property type="entry name" value="GroES chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020818">
        <property type="entry name" value="Chaperonin_GroES"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037124">
        <property type="entry name" value="Chaperonin_GroES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018369">
        <property type="entry name" value="Chaprnonin_Cpn10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772">
        <property type="entry name" value="10 KDA HEAT SHOCK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772:SF0">
        <property type="entry name" value="10 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00166">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00297">
        <property type="entry name" value="CHAPERONIN10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00883">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00681">
        <property type="entry name" value="CHAPERONINS_CPN10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00056825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ80701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="105" mass="11273" checksum="CA69D3A6476F36A1" modified="2017-01-18" version="1">MSSLLKSAKSIVPLLDRVLVQRVKAETKTASGLYLPEKNVEKLNQATVLAVGPGFTDSNGNKVTPAVKVGEKVLIPQFGGSPIKIGNDDEVILFRDSEILAKIQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1N0</accession>
    <name>A0A1E5R1N0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3996</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1N0"/>
    <dbReference type="InParanoid" id="A0A1E5R1N0"/>
    <dbReference type="OrthoDB" id="3972109at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF89">
        <property type="entry name" value="ZN(2)-C6 FUNGAL-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="9"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="812"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="817"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="855"/>
            <end position="871"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ80801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="923" mass="106043" checksum="F1604B5012288747" modified="2017-01-18" version="1">MPPIKKLPACAQCRKRKVGCTRERPECSNCLKRGDRCCYPESLDGRFTEILGESGRTNRVHKAATAGLLPTMDYLQKDRAARKNKRGNHESPMEIPRFGKFGSPSADEYGFMGHGQVXSGTNIITNTLXNTVPNTFDSLESSSRMNTPLPRFSSVQYPENSLNXXNALHKNEDFSFSNGYNGYTENLDGNASYKRPTSLEPEQVEIFDDLSRIMSSSKYDAVASYSKEELLDKQIMLLKAKLEQLTNWKKKNHINDSGFSSKTLSNNFHDKNDYKLEDAGLPRNSDACINKKESALTNEKLASFINNKFSTSVVDIDTEFFSESFGFKLFQNSTSPIFTLSKMFECSSELQKFRKTVFKTSKMKSFLNTSAAAASASEIVFEPAMEISFPENSSECGIFWCEIFQDTFVFSSHVFPQDMFSIFGNLRKFFKKLQDFDFQSKDQLTPDFMVQLIEYTFILLWSYYSNAYSSVFPQYEPSSVFYPYFQNLTLYVPVLENNIKYAEQYLVLNQSNKFLSFEHKFKVSLLFQIYYTYLKGNFSHQLPVLIGAFASYESKAAHLQLDHFLQLSFFQGDCSLELNHLGEFQSVQQKFITSVKKNNYNYSYQQLDGMLEEWKVSLQKNDKVEFDNIGDDLLGSGFENTSMMNRLDKHADDSYTVEQLLQSYKERLTFAHASLFINYYKLLDYQKHYYGDLTKLENETILFDKFLLSCLSTIGTSFDILSSTRLGQHQVLVSNFAYKALLALTTMLFSIRYKNPKLIKIILIKFRLLMIEQTSGLLNKYTYNTEINIALDLYLHHFGFEDTVDALMVHSTEQSENAEKKSAVEDNKTEQQPSEVTIPVESPAENTSAXKERAGDEEEEEEDDTEDDETETGNSEEKSSNENLLKRISFYLMDKNSFVKKDMPETYGVQDETVSEIYRFLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1P0</accession>
    <name>A0A1E5R1P0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">U1 SNP1-associating protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4161</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1P0">
        <property type="interactions" value="189"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1P0"/>
    <dbReference type="InParanoid" id="A0A1E5R1P0"/>
    <dbReference type="OrthoDB" id="4066580at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="276"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1011"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1040"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1041"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1061"/>
            <end position="1072"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1095" mass="126124" checksum="F399E204C2AC4ACB" modified="2017-01-18" version="1">MSSTHFVQAPLDSFKTMIVSVRIQNKNSQNDEVMEILRCEPNSTFEDLKKCIQYRHPGLRESRTFSIQSSSDTIPLATSELKNTLEQYASFSAGNHLKLLFSQQHEEDRSVLSLKDEDRAQETDFTNAVSIPIEMNVLSMQKRMSFPSWPHVSVTKTTFAQMLERSLQSLNTFEKQNAGTHSGNFCNLGAHTVTDIIGVCFNGTTYTKEQVSGLQNIFLNDVLGLDVIPSGRRADLMVYVKHGQGAPQSKTGENSEQPVEKXVLEFVSESELISQEMELYRSHTVEDVKEYICSVYAHATRINTKDVRLILHGQILEDNSVPMVSYYPRVSNGHEENDILPTRRFKVHVVINTDGFFRYDENEPGFWTELFHNPNRFAFMNRLYPQPEHSTRNLNNANAVFSNNTPEPVLRERSVSIPNVSPRAGAIVIAEDVVRSDEQDGLEELVFDDDEHAESAMKVNYEFCDGEGHDVQFDTEEPSLYRKVKVGSKDYLVEENLMQDSVFEYYLEFDDCGVENGSSQTGKLEVPLPPGSFQLHGNVVLFDRQIIRKINKLLHTKINSSFFASADSTTPSAVTTRPSTALNGFDMGNGNHHNSELYGVDLNGINSARMNDNLVIIPDSPVMETIKMLLFICCRSLYLVLRSSVLGIIIILQLSTFLPTNYTLTLMGLNFLRNFWVCDELWELWTDFFVLQRLSDYDYKNIHQEIIAQDDIELELLLQLKENPIVQRLLYFGVSQDWSRRETNYGNLDDSVGHEEWVKFGLYDFLNEETGEYALPNQQHTMEIIFNTLQHNPHLKHTFQYKKLIRYLILYFKQNLNSWKISGFQQTVHNHLEVAMNEDVNVRIGEENNYVYTFTNAMIGVDDFADIATPEKVKEVETRNIKDLKKMINVLRLFFRGRSVHRVAVNRLVGFFNTDLVNFITPQVNTEQTQPIPNXASTQHATSNIANRTDXNNNVQHDDNFVLKXIKIVIKTFVHIFQNVMLFVLVLLPHFQRVFEQIMLKREEEEERMQRVQEERLEHQQDDRLSSTDSVHDIAVNLLQGDEEEQEEQEEQEEQEEQEQLQRPESNQNDDINTAEDVIREIQGSTGYSHHEDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R212</accession>
    <name>A0A1E5R212_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g4382</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R212"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ80948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="678" mass="76900" checksum="7037961A3D68BF58" modified="2017-01-18" version="1">MNYQIEPWVVDFQHTKYDGGYALLGEKLHISQRSRKQLINCASHYIKESPHIINGNSTLNXLEYVLTTSSNSKKSSSILNLLECTSFEERKINRVSNKRQGLSTRDADFKERMLLGAAEKSLLVQVSSKLEPYKLQPITAIVSRKTLFLQNPSLPPXKKCRLKAGEPFKVPNFEVCTKYLQGNEPTPKEDEILVANEHFRLNVSYQLTELMXSSKSISTFHIEPIWNYKSTLNLSKIINFSLLTLPQLIEPLHSLYQKEVFDEDCDQRVLFKLSYPKSTIPSRFQVKLGKEKFLWKLATEQLKQMDWSIFRASMDRKRHXCSVLDEQLPVATPSALPFDSKFKLHVLADADLLLLPKSTSKTFTLTXQQPSLQKHTNNLPDTSFSSFVSRKKQKLQEQSNSNLNDXNINTSXISQLMVAGMDSSTMTEQNMSSIIQNKKTLPXLIKERACNIKSPFPESQEQIKTIVXNYELIDSQVLSGLYAXDESLNIIELDKAAFQSPCEIALNPDTCVIFIDVFRFFQKSSSGNVLYYIPILXKLLVEFQKVYCLITNIAVNSAENAYKCQTVMQSLYSAQPEVVILPTFDSKMTVSTIVHLXYYHDTFLPSTIANLNNGCSFNEMLLLGMGFNLAVAKHILSQTPNFAYLIEHLDDGLTEVSPKQLAKIKNVLLTPFAALPNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R238</accession>
    <name>A0A1E5R238_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Endoplasmic reticulum chaperone BIP</fullName>
            <ecNumber evidence="4">3.6.4.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Endoplasmic reticulum chaperone BiP</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Immunoglobulin heavy chain-binding protein homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g3923</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Is required for secretory polypeptide translocation. May physically associate with SEC63 protein in the endoplasmic reticulum and this interaction may be regulated by ATP hydrolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.10" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R238"/>
    <dbReference type="FunCoup" id="A0A1E5R238">
        <property type="interactions" value="1375"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R238"/>
    <dbReference type="InParanoid" id="A0A1E5R238"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10241">
        <property type="entry name" value="ASKHA_NBD_HSP70_BiP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000009">
        <property type="entry name" value="DnaK-type molecular chaperone BiP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000172">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000026">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042050">
        <property type="entry name" value="BIP_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="664"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="301"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="673"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ80961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="692" mass="76334" checksum="A8FAA059C886EEBF" modified="2017-01-18" version="1">MNYSKKSISLFGIFVYALLVVVLPLSSKFNSNNSRSSSLVYGASSDDTNYGTVIGIDLGTTYSCVAVMKNGKTEILANEQGNRITPSYVAFTQDERLIGDAAKNQAASNPHNTIFDIKRLIGLKYDDKSIQRDLKHLPYKIVNQKGKPVVEVSLNGEDKSTSKTADTKIFTPEEISGMILGKMKQIAEDYLGEKVTHAVVTVPAYFNDAQRQATKDAGTIAGLTVLRIVNEPTAAAIAYGLDKTEDEHQIIVYDLGGGTFDVSLLTIENGVFEVQATAGDTHLGGEDFDYKIVRYLTRIVKKKYDIDISSNERAIAKLKRETEKAKRALSSQMSTRIEIDSLVDGIDFSETLTRAKFEELNLDLFKKTLEPVKKVLKDANFEKTEIDDIVLVGGSTRIPKVQQLLEQFFDGKKVSKGINPDEAVAFGAAVQGGILSGEQGVEDIVLLDVNPLTLGIETTGGVMTTLIKRNTAIPTKKSQIFSTAVDNQPTVSIKVFEGERSLVKHNNLLGTFELTGIPPAPRGIPQIEVTFILDANGILTVSATDKGTGKSESITIKNEKGRLSQEEIDRMVEEAEKFAKEDEEIRLKIESRNKLENYAHSLKNQVDTGDLSDKLDEEDKETLLDAVTDTLEWLEDNMDSATSEDFDEKFEDLSKVAFPITSKLYQQAAPGEGPEDDDEEDEFEDDDYFDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2A0</accession>
    <name>A0A1E5R2A0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP synthase subunit 5, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3944</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase delta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2A0"/>
    <dbReference type="FunCoup" id="A0A1E5R2A0">
        <property type="interactions" value="702"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2A0"/>
    <dbReference type="InParanoid" id="A0A1E5R2A0"/>
    <dbReference type="OrthoDB" id="1262810at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.20">
        <property type="entry name" value="N-terminal domain of the delta subunit of the F1F0-ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01416">
        <property type="entry name" value="ATP_synth_delta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026015">
        <property type="entry name" value="ATP_synth_OSCP/delta_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020781">
        <property type="entry name" value="ATPase_OSCP/d_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000711">
        <property type="entry name" value="ATPase_OSCP/dsu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01145">
        <property type="entry name" value="ATP_synt_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11910">
        <property type="entry name" value="ATP SYNTHASE DELTA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00213">
        <property type="entry name" value="OSCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00125">
        <property type="entry name" value="ATPASEDELTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47928">
        <property type="entry name" value="N-terminal domain of the delta subunit of the F1F0-ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00389">
        <property type="entry name" value="ATPASE_DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ81022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="206" mass="22284" checksum="73156ED6B0780A0A" modified="2017-01-18" version="1">MFTRSAIRNLATAAKVVKPPVQLFGLDGTYATALYTASMKASSIDATYDSLNKLSDMIKQDPKISSVLENPSLSQQERLEVVKILEGKIPKIDQSVSNFLDVLSANNRLSLLSKINSSFQQLYSAHNGVVEANVVSAVPLDSKILKRLNKAISSSSLIKEGQTLKISNIVNPDVQGGLVVEVGDRTADLSIATKINKLNQLLNETI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2G2</accession>
    <name>A0A1E5R2G2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FAS1 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4081</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2G2">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2G2"/>
    <dbReference type="InParanoid" id="A0A1E5R2G2"/>
    <dbReference type="OrthoDB" id="5551751at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040200">
        <property type="entry name" value="Mug57-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28156">
        <property type="entry name" value="FAS1 DOMAIN-CONTAINING PROTEIN YDR262W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28156:SF1">
        <property type="entry name" value="FAS1 DOMAIN-CONTAINING PROTEIN YDR262W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184573" evidence="2">
        <location>
            <begin position="22"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="285" mass="32901" checksum="A16ED653E4D891E0" modified="2017-01-18" version="1">MKTQLLFYTALLLAIMGAGFAKNVSPVKMVDGKKHLDIEPSKLEKRDAKRVVLDLAAIAEAGKDWDTIKWKRSFVEQKELEEQGVLSIKHRPLTTSISLNPKTSIFYDYCKNVVPLYDYLNDFENDTDEDAVTDLGREKNVEHGRSDYQFLVFTPTNEAVQRLSKKPWEFPIDIDKLENENAEEKVIQDSIEENSNRFVYEHVVLLNAQDANHQVPQNAEKLLKDHYLXLTTLAGEKINLVHDSTQPASFYIKCLRNKNRHIRVLEYSCYRNGCVFVTDDCLIEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2N3</accession>
    <name>A0A1E5R2N3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Elongation factor 1-gamma 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3922</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2N3"/>
    <dbReference type="InParanoid" id="A0A1E5R2N3"/>
    <dbReference type="OrthoDB" id="249703at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1010:FF:000001">
        <property type="entry name" value="Elongation factor 1-gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1010">
        <property type="entry name" value="Translation elongation factor EF1B, gamma chain, conserved domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050802">
        <property type="entry name" value="EF-GSTs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001662">
        <property type="entry name" value="EF1B_G_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036433">
        <property type="entry name" value="EF1B_G_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986:SF1">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00647">
        <property type="entry name" value="EF1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01183">
        <property type="entry name" value="EF1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89942">
        <property type="entry name" value="eEF1-gamma domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50040">
        <property type="entry name" value="EF1G_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3 6" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="2 3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST C-terminal" evidence="5">
        <location>
            <begin position="78"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="EF-1-gamma C-terminal" evidence="4">
        <location>
            <begin position="236"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ81166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="413" mass="47329" checksum="F3A2F672AE6F6378" modified="2017-01-18" version="1">MGQSIPKAICEYFKLDSVAALSKVNAHRYSXSSNGLYNLTDVLAYIYALLIPLADKYKETGASHYXLAYKLVNGEKNHESTKAVNVMWIKFVCIRILVPFADAMKTLLENVATSKSQFDAKNASIHETVNXDFESILSKQDYLSVEGPTLADFFACFSLAGAFDGFWKSEWIAMHPATFEWFKRVQNHPFSKSFFTGLVLTENHSNSSVPNDNKIPKKVDTNNASDIPKFKFIRKXKHPLELLGKSTFNLDEWKREYSNNDTRXVALPWFWEHYNPEEYSIWKVSYKYNDELTLTFMSNNLVGGFINRLSGSIKYMFGCLVVYGENNNNGIIGAVMIRGQDFKPAFDVAPDWESYDYEKLDVSTEENKEFVNNMWAWDKPVVINGESKEIADGKVLKNYLTQTPWVEVYNEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2N8</accession>
    <name>A0A1E5R2N8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ATP synthase subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4209</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha(3)beta(3). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the ATPase gamma chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2N8"/>
    <dbReference type="FunCoup" id="A0A1E5R2N8">
        <property type="interactions" value="956"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2N8"/>
    <dbReference type="InParanoid" id="A0A1E5R2N8"/>
    <dbReference type="OrthoDB" id="239812at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12151">
        <property type="entry name" value="F1-ATPase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.80:FF:000001">
        <property type="entry name" value="ATP synthase gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1380.10:FF:000003">
        <property type="entry name" value="ATP synthase subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.80">
        <property type="entry name" value="ATP synthase, gamma subunit, helix hairpin domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035968">
        <property type="entry name" value="ATP_synth_F1_ATPase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000131">
        <property type="entry name" value="ATP_synth_F1_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023632">
        <property type="entry name" value="ATP_synth_F1_gsu_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01146">
        <property type="entry name" value="ATPsyn_F1gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693">
        <property type="entry name" value="ATP SYNTHASE GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693:SF22">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT GAMMA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00231">
        <property type="entry name" value="ATP-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039089">
        <property type="entry name" value="ATP_synthase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00126">
        <property type="entry name" value="ATPASEGAMMA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52943">
        <property type="entry name" value="ATP synthase (F1-ATPase), gamma subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00153">
        <property type="entry name" value="ATPASE_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="10 12" id="KW-0139">CF(1)</keyword>
    <keyword evidence="5 12" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ81150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="311" mass="34253" checksum="68C0486D8068A283" modified="2017-01-18" version="1">MYRTTFNTCLKQQRTYATLREIENRLKSIKNIEKITKTMKIVASTRLTKAEKSKVSTNEYVKAEKKFFETIEADKTDLTGAASAEESAATGKPVSLIVAITSDKGLCGSIHSQLAKTIRNKLNAMDQETLANTELVTIGDKIKVQLTRMFPDNIKLGFNGVGKAAPTFEESSLITSEILKYVNEESGSNKKYGKIEIYFNNPVTSISFEPTEAPVYSGDSIIAAPGYSKFEIDNDLGVPRDLFEFSLTNKILSAMANGYAAEVTARRNAMDNASKNAGDMINKYSILYNRTRQAVITNELVDIITGASSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4P2</accession>
    <name>A0A1E5R4P2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">prephenate dehydratase</fullName>
            <ecNumber evidence="2">4.2.1.51</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3816</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-phenylalanine biosynthesis; phenylpyruvate from prephenate: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.51" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4P2">
        <property type="interactions" value="131"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4P2"/>
    <dbReference type="InParanoid" id="A0A1E5R4P2"/>
    <dbReference type="OrthoDB" id="983542at2759"/>
    <dbReference type="UniPathway" id="UPA00121">
        <property type="reaction ID" value="UER00345"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004664">
        <property type="term" value="F:prephenate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009094">
        <property type="term" value="P:L-phenylalanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04905">
        <property type="entry name" value="ACT_CM-PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13532">
        <property type="entry name" value="PBP2_PDT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008242">
        <property type="entry name" value="Chor_mutase/pphenate_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001086">
        <property type="entry name" value="Preph_deHydtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21022">
        <property type="entry name" value="PREPHENATE DEHYDRATASE P PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21022:SF19">
        <property type="entry name" value="PREPHENATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00800">
        <property type="entry name" value="PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001500">
        <property type="entry name" value="Chor_mut_pdt_Ppr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51171">
        <property type="entry name" value="PREPHENATE_DEHYDR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0584">Phenylalanine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Prephenate dehydratase" evidence="8">
        <location>
            <begin position="29"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="9">
        <location>
            <begin position="293"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ81886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="383" mass="43363" checksum="031CF5EACC744E42" modified="2017-01-18" version="1">MSRQSRIKKKKKQKQKATTSIHKIIEMTXVVFLGPVGTYTHQAVLQQFKQDDQADCAELIPLKTIPDCFSRVMEDESVDYCVVPFENSTNGQVVFTYDLIRDNIIDNLANEDKLDAQKNDSIAGSTETAHFATRIVPPLTIVAEQYVPIEHCLVTATQELGELYSQQDNDGDTEPVYENLYSHPQVWGQVSQYMENXLKGKFLNXIDCTSTAEAIKMALQDYKLYGKKSLAIGSKIGAMLNNAYIIQEGINDLKGNTTRFLVLQKGGGLALIKDPQSKENQLLDIRQKMMTLLTFTTTREGPGSLVDVLNIFQKYNINMTSISSRPLGSINDKNWSYIFFVEYEYNTSLPWKAEILSQIDAKCTFWCMWGVFNRNSAYYKSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4P8</accession>
    <name>A0A1E5R4P8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3802</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4P8"/>
    <dbReference type="FunCoup" id="A0A1E5R4P8">
        <property type="interactions" value="368"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4P8"/>
    <dbReference type="InParanoid" id="A0A1E5R4P8"/>
    <dbReference type="OrthoDB" id="10250725at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF13">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="34"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="203"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="226"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="245"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ81851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="425" mass="48209" checksum="A9558E1535A73689" modified="2017-01-18" version="1">MSRISAGNKYSNQFNYNGNGMANNVNPNTLNKRYQVLETIGSGSFGSVKKIYDYETGTYLVRKDIKFAHMSLKERQQLINECKILSSLKHNNIVEFVSFDYDTDTVYIYMEYCDGGDLAKLIEAFREKKKRIPELIVWRILVQVSLALWRCHYGADPPLLGTVFDKLKKPDNSDIASSQIVIHRDLKPGNIFLTNLHIGPYGSSSSTTTTTTTTTKSNSASSLETSPPPSFPPYPTSLPPHAPRSTRTMSYSPNATPHDNRSGGVDQVIAKLGDFGLAKSLKSKNHFATTYVGTPYYMSPELLKDKPYSPLSDVWSLGCVLYEMCCLRPPFKGAKTFTELESRIVSGVYEPISASYYSKNLRTIIDRCIQVDLRKRYSVMDILNDIQAKIMYKSLQLEMFESQLLQYEKELVNIEKHLEQRYMGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4U2</accession>
    <name>A0A1E5R4U2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3514</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R4U2"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013743">
        <property type="entry name" value="NBP1/CSA1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08537">
        <property type="entry name" value="NBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="87"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="417"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="282"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="433"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="453"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="473"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="489"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="508"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ81912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="518" mass="59882" checksum="51236AB5001C5149" modified="2017-01-18" version="1">MSFVSKLKGKMNNFLDYLNEEDDPKEQIEHLKKYDDYSTDEEHKXSRDQDTDNNNKLDDLNKXKFENLINKETSATRLTRKQYLMKKRKEHGSPIRNRIGKNTTQFRKGTGKGKSXGXASLRSTTSXPKIQDKRTVPAKIAKNVVNFVRNVFTTREQEVREMNNEYVVKDIGDFNLDFRNKQRSKYMRPEQNKKMMLNNEEFRKFVMRSKYDAMMKKQLQESIKNSDAREIAKNTISFAQQGELDDGARXNGSFNLNSDNFDYQKDISGSSKEFESVAEKENFELQKKIAHYKKELAKKEQQLKTSIDKIRXLEGVIDDMDISRIENTSAAQSKWTDMDNEYNIDESTDFGLETGYKNTVLGFEKKPLAKMSSXSKLDNTENKKEDSELINSRIKIKKNLVESRRRITNLPEAHVPVLDTLPPNLSPVKEPLFTSSPTRKPSGNKLQPYTLGDIEDFDQESKEDSDENGNVFKKNDERTSRKLDKENVHLSNGLHNATELSPIRVDYSKYSSSPSHTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R535</accession>
    <name>A0A1E5R535_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">inorganic diphosphatase</fullName>
            <ecNumber evidence="3">3.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pyrophosphate phospho-hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3736</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R535"/>
    <dbReference type="InParanoid" id="A0A1E5R535"/>
    <dbReference type="OrthoDB" id="1608002at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="INORGANIC PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ82002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="246" mass="27892" checksum="A40AD97C0AFB22DD" modified="2017-01-18" version="1">MVYTIKKVDLKNSVNYKVYVERKNGKPISPLHDIPLNVNIDQQIFNMVVEIPRWSNTKXEISKKEKWNPIXQLAEKVGQGFAKTGSAQKECFRNYGVFPQTCQLSNQAHPSKKVKVLGVLELEDEDKRDWKXIVIDVADPLSSKLNDIKDVERHMPGFFEAANNFFGVCEAVNGEGKPPVSLFWCKNYALTLIRKCNKAWRKSMRNKVDLGNIALTDTTLKDAEPCTISADSLNFSTSTSTKYKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R537</accession>
    <name>A0A1E5R537_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">GPI transamidase component GAB1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3507</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGU family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R537">
        <property type="interactions" value="660"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R537"/>
    <dbReference type="InParanoid" id="A0A1E5R537"/>
    <dbReference type="OrthoDB" id="549017at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009600">
        <property type="entry name" value="PIG-U"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13121">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13121:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN ANCHOR BIOSYNTHESIS CLASS U PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06728">
        <property type="entry name" value="PIG-U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="310"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="372"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="407"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ82020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="449" mass="51837" checksum="736C5B9C9164AC53" modified="2017-01-18" version="1">MLKKKNIKVNSPSNSVPTLSMKLQVILIYLALCFVSRYTIFQMFPSLSETLDNTVEFSTPISSFKTLREGWYLISKGLPLYYKKNGGIINQPPLLIYLLKPLQRKIDVVFCLMDCFMMFLFIQMVSVFNKTXGNFLKTKSQGSDEKAPNSXMEQKIAIWYLLNPLIFLNCISKSTTIFNHVFILMAVLAGMKKSFVLSGLCIAIGGYINVHSLYLIFPLLNFTVFSKXGGTAKTPTVQVDKSHILTLVFSFSATVALLLYASYTVCSNQWWFLYNVYFVNLSFDKIFPNIGIWWYFFIEIFKFFRPFFKMVFNLFSASFIIPFTIRFHKSQQTLYCFVICLGWLILTKPYPTFGELGFFVQFLCFFDPIKCYFTYSIVSVLIFLHTLILAPIFYYLWISLGSGNSNFFYAITLVYSVGLASVLVDMLWAMLRLEFDNGEPQPEKKVVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R558</accession>
    <name>A0A1E5R558_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative secreted beta-glucosidase SUN4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3716</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SUN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R558"/>
    <dbReference type="STRING" id="56408.A0A1E5R558"/>
    <dbReference type="InParanoid" id="A0A1E5R558"/>
    <dbReference type="OrthoDB" id="5339822at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051526">
        <property type="entry name" value="Beta-Glucosidase_SUN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005556">
        <property type="entry name" value="SUN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31316">
        <property type="entry name" value="BETA-GLUCOSIDASE-LIKE PROTEIN NCA3, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31316:SF2">
        <property type="entry name" value="BETA-GLUCOSIDASE-LIKE PROTEIN NCA3, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03856">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013311961" evidence="5">
        <location>
            <begin position="16"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="105"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ82040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="416" mass="43260" checksum="24873471F136C734" modified="2017-01-18" version="1">MQALSLLALISVVAAAPVHNRNIGDVDHKPHHRHQDKRNVGDNNAAYTVVEYIGGDGKVYSTATIDDSTASTAESTQSATGAPAVALVNNDVASSASASASASATATSATATSASATATATSSDDSSSSDDDSSSSSSSSGISGDLSSFSNPTEKFQDGTVKCSSFPSGQGVISLSWVGYNDWASIMNMDGETSSSCEPGYYCSYACQAGMSKTQWPSEQPNDGRSVGGLYCGKDGYLYRSNTDSDYLCEWGVESAFVKSQVDKDISLCRTDYPGSENMVLPTLLQANDDSNGVPLSVVNEDSYYKWQGKPTSSQYYVNNAGVSVEDGCIWGTSSGSVGNWAPLVIGSGSSNGVTYLSLIPNPNNNDAPNYSVKIVGRDGSSTVGDCKYENGKYNGDGTDGCTFSVSSGIADIVFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R563</accession>
    <name>A0A1E5R563_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Putative ABC transporter ATP-binding protein/permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3738</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R563">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R563"/>
    <dbReference type="InParanoid" id="A0A1E5R563"/>
    <dbReference type="OrthoDB" id="66620at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF119">
        <property type="entry name" value="ROA1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="515"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="616"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="647"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="682"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="712"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="752"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1182"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1215"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1256"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1297"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1326"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1354"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1405"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="129"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="846"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="112"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="11">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ82032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1432" mass="162576" checksum="8B539BA1554E9D9C" modified="2017-01-18" version="1">MSNSDHSSFPGPVSILPAESELASTTSSSSSSEEEYFLLNDLVDNDLNIFVEKTNQGTTSASPPSSTSSSLHKNQAYLSKKDKGKKXKDQLFDFKKGNVSFHIRHLTXSTNDAKKDKSPGDAKTDEQTDQTASFLDNGNKSIILDDISVDIPESCICGIIGGSGSGKTTLLNYLMNTYNNNINSKNVLNSIKSKLPFQNYALHTEGDYYIKSTEYDDRYDGSDLKHKSSAKNEIRIAYLQQYDFLPNDLTPLEILTYTADLKLNHFSKEKKAEIVEMLLMELGLKDCKHIMISNAASQSNSGGDSSKTKSTLSGGEKRRLGIAIQMISNPKILFLDEPTTGLDSYSAILLLNTLKKLCKLYGTTFILSIHQPRADIFSKYLDQLIILSTGKLVYCDRADKMIDYFTDQCNIPLPLNFLNPCDYYIDQCSIDNRSSDLYQESSQRLGKLERIWKSYRTLHEIEKEVITDDNSSNNELHNNGRVLKNVVFVNPHVNRWKQIQVQFKRTLKLSWRDHWTFVGVYLEPIVIACITGWIFYRPVKSINRDLPESQQPNSSQIRTLSASLYTAGALQGYLFLLFETYRLCSTDIKLYDMERLKTNRVITPFTFLVARKLAKFLLEDLIVPFILSIITYFMYGLNSSHGNHNSTFVNFLIYFSIILVNHQVSTNLALLSVSISRNFAQASLVGNLFYTLQSIAGGFFVNSKEMPVYVRWTKYITFFWPSFGALISNEFTDSSLTTYNQFVVSLGFPLHWITAPIIIVLCWAIGFILVAWMIFNMVTFDFNQINSSNIKFQSKTTRKQHGTDIEKSSLSSDTESIVESKEVLKFDLTEEEEQKSKAAIKKGLDIELKDFSLRFKEKYILNNVNAYFPKNQLNVIMGPSGSGKSTLLNVISNRVGHSSNYKMDGTIYLANTAIEPRELNKYCSYVKQHEDHLLSRLTVWETLYYAGRLRLPDLEDKEIRSRVEQLIMDLGLWHCKDVLIGDEFNKGISGGEKKRVSIGVQLLTSPEIILLDEPTSGLDSFTASTMMNLLKKISERTTVIATIHQPRSEIFEAFANVLLLSKYGKVAFNGSPFAMKDFFMNNLHYKCPKLTNIADFYLDLISLNTQNDTNERISQLRVDSILKEWEAYRSTHLLNPNSAHNDYGNKFGAVPHRETKTVPLYTIYQICLIRQFKNLVRDLDPLVARLVQVPAMGIILALYFSPLKHRGTSAILNRFGLVQQATSLYFVGMLSNLAIYPGERNFFYNEFQDGVHGGSLLPFFCSYLTIELPMSVFGSLIYTIFSVLVTGLPRNATNFFWTFYGVFIFVTCGESLGILTNTLFPQPGFVVNSISICISLGCCMSGIMSLNMDVVLKVINWFSPMNFVIITLINHAFPESLQIKLDPQTIITGREILQNYGIYKSKDYYMIYATLVMIGYRLLAFVVLWWKLRSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5D0</accession>
    <name>A0A1E5R5D0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">N-terminal acetyltransferase A complex subunit NAT1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3807</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5D0">
        <property type="interactions" value="1104"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5D0"/>
    <dbReference type="InParanoid" id="A0A1E5R5D0"/>
    <dbReference type="OrthoDB" id="10263032at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021183">
        <property type="entry name" value="NatA_aux_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767:SF2">
        <property type="entry name" value="N(ALPHA)-ACETYLTRANSFERASE 15_16, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12569">
        <property type="entry name" value="NatA_aux_su"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000422">
        <property type="entry name" value="N-terminal-AcTrfase-A_aux_su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="259"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="652"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="652"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="663"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="5">
        <location>
            <position position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ82115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="876" mass="100348" checksum="4A3E7124D9F94802" modified="2017-01-18" version="1">MSRRKIGGASAPKKKTAGTNGVSSSMATAIIGTPKENAQLAEAVKLYEGKHYKKSIKAVDAILKKNSSHFDSLSLKALNLFFINDKDQAESYVKKAISKINGNTSLCTHTGCFILGLYYKQTKNYPESAKWYQLSLDNGSPNKHSHRELAVLYAHLRNFDGAVKERTNYLQAMMGYRANWTGLALALDLAGKPEQAAKYLANFEDLAKGKLGPGEMYENNECIAYKNDLYYKMAGNDPEKLQNVLKDLDTIENDVYDKYALLERRANIYMQLGDMNNASKVYRKLIKRNPDNFKYYKLLEVSLGVTDSPSLRLALYEKLQEFYPRSDPPKYMPLTFVQDLKKLEECLSAFILPQLNRGVLATFNNIKPLYQKRSNTVPKLVETIVESFLQTDADPTKKPDVHLWTLYFLAQHYLHTKNFVKAQEYINKAVEHTPTAIELYIVKARILKHLGLLDDAAKVVEEARQLDLQDRYINNKSXKYHLRSNDVDKAVDLISLFTKNDKEDLNGIKDLHTLEAGWFLVEQAESYYRLYLSTKKQILEKLRSAPAIEVSDECFDELKYDMTKYKGLALKRFAAISKIYDNFNDDQYDFHSYCPRKGTARFYLDLVSWGDKLYTLPIYVRAMKGFGKIALDDESKDEKLIFPEDVGKSANTKKKTKKSLKQKQTDDELKVASEKKDQDPLGEKFLHAKSPVHQFLDIFYEKYSKQVLAEEKDELLEFMVNFRLGKFAICVSAMNKILQRRTAHSNRALVGAMCMMLVTVLETQAENLDPISKAVLQRSLNEQFKFSRDLLLNDDAATGSSPKQTSTEDKLDWFAYLQKTFGISSESLLLLYRFFSEQDDVTGQKYIXVDELKPHILDSIKAEDPIEQSNILNYEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5E1</accession>
    <name>A0A1E5R5E1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zinc finger protein RTS2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3587</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5E1">
        <property type="interactions" value="339"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5E1"/>
    <dbReference type="InParanoid" id="A0A1E5R5E1"/>
    <dbReference type="OrthoDB" id="10266249at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2030">
        <property type="entry name" value="DNA/RNA-binding protein Kin17, conserved domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056767">
        <property type="entry name" value="C2H2-Znf_KIN17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019447">
        <property type="entry name" value="DNA/RNA-bd_Kin17_WH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037321">
        <property type="entry name" value="KIN17-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038254">
        <property type="entry name" value="KIN17_WH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12805:SF0">
        <property type="entry name" value="DNA_RNA-BINDING PROTEIN KIN17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12805">
        <property type="entry name" value="KIN17 KIN, ANTIGENIC DETERMINANT OF RECA PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25095">
        <property type="entry name" value="C2H2-zf_KIN17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10357">
        <property type="entry name" value="WH_KIN17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01253">
        <property type="entry name" value="Kin17_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="248" mass="29001" checksum="3F45F04DF8B4750A" modified="2017-01-18" version="1">MXEFHKHQRKQQNSKRLSKLAFYCQICQKQCRDDNGYRQHLRSPHHLKMKSKITSKDIAEYNDLFEKSFLQYLRLFHGEKWVNANKVYNAFIINDKDHVHMNSTKWSSLTKFVQHLGKAGKVHVQLDENEQADFYSKMDNNDAVNCNSLMISYVDTSSETLLRKAQVAQLERDNLTEQESRALLLQKQMDDAKKLLEKEEEEEEEEGAAKDDETTFVEPLGKISLVTSSRVGKPKGKKFVKKKKNVFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5L6</accession>
    <name>A0A1E5R5L6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Trans-aconitate 3-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3493</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5L6">
        <property type="interactions" value="733"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5L6"/>
    <dbReference type="InParanoid" id="A0A1E5R5L6"/>
    <dbReference type="OrthoDB" id="3970735at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051052">
        <property type="entry name" value="Diverse_substrate_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942">
        <property type="entry name" value="METHYLTRANSF_11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942:SF4">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="4">
        <location>
            <begin position="47"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="5">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ81833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="303" mass="35005" checksum="45C9ABE19277F352" modified="2017-01-18" version="1">MSQFASKEFDGHNYDTYRPDYNPEFYNYLLAYHQRGQVNGTETRTILDVGAGPGTATFQMERILHDNFDKVIGTDVSPVMVDTANSKLKLFNAARSAKERAKLEFSVSSCMDLSEHIQXKVDMVTAAEAAHYFLPFKDFLKEAKKVLKPHGTLAVWGYMDPSFVEYPEFDVLIQELQYXKKSLGDYCDQPGVTXMRNLLDAQXIDESDGFYDIECEKFYANDFRKNNKKGFPLIMQREMTITQFKMLXTTWSTVSKRESVLHDTETIINNFFEKMFSLALELDWDTQVTVVWNSVYKFARNKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R6B8</accession>
    <name>A0A1E5R6B8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nuclear envelope morphology protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3591</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R6B8">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R6B8"/>
    <dbReference type="InParanoid" id="A0A1E5R6B8"/>
    <dbReference type="OrthoDB" id="277011at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000093">
        <property type="entry name" value="NLI interacting factor-like phosphatase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="FCP1 homology" evidence="3">
        <location>
            <begin position="250"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="7"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ82083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="462" mass="51611" checksum="36CA2E9D4D8496C5" modified="2017-01-18" version="1">MNVIGYISNYIPQPPTSSKQHQESTNEGLVDPENGSKHQETARESQGVSFHHEGTSLEMDQLIRTSVDNNLTSAYSSGPLPKRTNSLNKRLSQVYDNTADELALQSQTTTIGKPDVTAAAAAATSTSTSTSAATYVSTLYKIWRVLIFVPHLLIVRPVLFVWYIVTFPLTIIEMISTFALKSSDNTQPSPHPQAPNSQSSATDLSHVNSVTLKQDFIKDGTPTPSTQLSRDFGGKFFFPKKLIPNSIMNNPNKKKTLVLDLDETLIHSVSKTSNMSNKAHVVEVQFVPSAIPPIXQNTTSLKQRRGTTNNNNSSANVISTLYYVNKRPYCDLFLNKVCKWYNVVIFTASIKEYADPVINWLESSTRARFVQRLYRQDCTLKDGIGYVKDLSILQHSLDDVVIIDNSPISYSMNIDNAIHVESWINDPTDSDLLQLLPFLEAMRYTTDVKTVLCLKRGEEVFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7G6</accession>
    <name>A0A1E5R7G6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">GPN-loop GTPase 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3159</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Small GTPase required for proper nuclear import of RNA polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Binds to RNA polymerase II (RNAPII).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7G6"/>
    <dbReference type="FunCoup" id="A0A1E5R7G6">
        <property type="interactions" value="905"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7G6"/>
    <dbReference type="InParanoid" id="A0A1E5R7G6"/>
    <dbReference type="OrthoDB" id="5839at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17872">
        <property type="entry name" value="GPN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000552">
        <property type="entry name" value="GPN-loop GTPase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030228">
        <property type="entry name" value="Gpn3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF7">
        <property type="entry name" value="GPN-LOOP GTPASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ82836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="271" mass="30600" checksum="691F326EC310082D" modified="2017-01-18" version="1">MSKVGILVLGPAGAGKSTFCNSIISHMQTIGRRAHLVNLDPAAEPSKYEFTVDIRDLISLDDVMDELEMGPNGGLIYCFEYLLENLDWLDEELGDYNDEYLIIDCPGQIELYTHIPVLPTIVRHLQQQLNFQLCATYLLEAPFVVDTSKFFSGCLSAMSAMVLLELPHINILSKIDLIKDEYSKKRLKRFLNPDPLVLTDSANKDTSSKFHHLNTAIANLVDEFGMVQFLPLEVKNPDSLNTILSYLDDVTQWSESQEPKEPADQFDIEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7J3</accession>
    <name>A0A1E5R7J3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Bud emergence protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3432</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7J3"/>
    <dbReference type="FunCoup" id="A0A1E5R7J3">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7J3"/>
    <dbReference type="InParanoid" id="A0A1E5R7J3"/>
    <dbReference type="OrthoDB" id="4059796at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ82879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="629" mass="71124" checksum="015AE6537802ACF8" modified="2017-01-18" version="1">MDYEKLLFDLGPILECGKSQNDCLDYSNTELQNSLKSHLDDLAVALRAPNNRVILKDSGVLAHLISSCCVVTSTELQSDIIRCLANCVIDNDANRNFYIDCEQSTKLNEKLCVNIENIVSNNEDPFEIGKRSVIFIRNLCLEEIPIALCQLFISSFGKLFFHIVASVSELMSGDTGLYLLIFEFYSDMVKMCVEKDSLRSCIFNDVSSLPTGIEEIANVVEHFATLVLKMSNQFEQSFAEEDREDQDIEEPEELTFLVLSSQLLESFICNFEQSCVKRTPSHAYNSIFSTLFSTLSTLECIPQFPSKLITLRRISYCINTLSTACQSSFADTDFFVQLFSEPQDAGKKFNYTYSSLFFCLSNCVLSSKDATGLLKKIAATSEKSFHNLISCFRGNILNTDPWTYQSFLDLMRKLVTVVHYPENSDDLSTLWKMLQAVQASLQVVSGLDLYLFKLLEKLVAVLPGKLLNESGFPVKDLILQTPTGSSNTSMHILGCLTLAKMYNYLQPELLFSLVQFSLMPCLSNGTLHNYDEQYLFQLFKCFGLLLQNYSFSSEQLESLEFQQLLEKLYEVIVHVNEKSNTKTAADQAVRNNCVFLCGVLINKFPSFFETSEDPSRLSYLKHIIVEATT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7Q6</accession>
    <name>A0A1E5R7Q6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3232</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7Q6">
        <property type="interactions" value="248"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7Q6"/>
    <dbReference type="InParanoid" id="A0A1E5R7Q6"/>
    <dbReference type="OrthoDB" id="541276at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF32">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="10"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ82949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="745" mass="83888" checksum="8E4D7F836C4447C4" modified="2017-01-18" version="1">MLHNCQINNFQITKQIGSGAFGLVFHAIDLISENEYAIKAILKNPQQSNKVSNQEKVKKSTLLQNQLYHFFKSYQNKLFLPSINLDSIKNLNDADLLLAQGSTNGINGVEHAAYYKEIALHLRVHDHNNVVTIHQILESSLATFIVMDYYPIDLFTSIVDKQHFSQDPLLIKKVFLQLCSVLQYCHENHVYHCDIKPENILLDKQDNVYLCDFGLSTIQPCLPMNVCVGSSYYMPPERSPSCEKYLKKTHQLQNNDNKMNQLSVSPQATQFPTALGDVWSIGIILINLTCIRNPWLKADCANDNTFQYFLKDPSILKKILPVSEDLYQVLLKVLKLNPWQRISLNQLIMEVAMLKCFNNNKDSPLYHAPILTLEQIETFTNYNKQEIYYKKLMENYDQKGLYSSNNSTHGIVSNPQVASGNVISNTTMAVPDSEHFSEESRCNSNIFDSMVAPPVPATPTTVNAYQPPHYTAKNTNNSNNSAFGNHQYTNFNNXXPSLYSQNNVASQPINPLLNHPQNFLPSNFAVNHPVNAPLNNSTEFHPQNNNSFLYGNVNNNNLQQQQPQPQLVQQIHPVQHPVPQQQLQFHQPQPHHYHSHAAEPSTDFITATSRDTTPNDSMEKLVDYNLNNKTQPAQSXLGNGLNLGFGVYNKNAQSNTSSSSVAELKMKHMIVNHLNSINSDTNSVTQSLNNFLTKLTTPVSSPVPQTSGTADANASVAEAHVDYKSSEIHGQASENQYLPNYKNLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7R6</accession>
    <name>A0A1E5R7R6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Thiosulfate sulfurtransferase TUM1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3438</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7R6">
        <property type="interactions" value="447"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7R6"/>
    <dbReference type="InParanoid" id="A0A1E5R7R6"/>
    <dbReference type="OrthoDB" id="270167at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01448">
        <property type="entry name" value="TST_Repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01449">
        <property type="entry name" value="TST_Repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045078">
        <property type="entry name" value="TST/MPST-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364:SF27">
        <property type="entry name" value="SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364">
        <property type="entry name" value="THIOSULFATE SULFERTANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="20"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="186"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ82959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="312" mass="35302" checksum="11F78D0A205B8604" modified="2017-01-18" version="1">MSLFKIINPQEFIKLTKTVKAAKVIPVDCSWYMPNMKRDGKQEFLDTERLPGAVYFDIDGVKDKSSPFPHMLPDLETFNFEMGKLGLHETDVLVVYDKLGNFASPRAAWTLATMGHKDVFLLNNWNKYIANKDYPLETTKLSEFSRYPKTSYKSSNTHVSEQLLTYDEIKKLXVSGKLKELYNTYDARSMERYTGQAPEPRAGMPSGHIPMVQPLPFTEVLDAKSKEFQDSEQTKSKILSFFEKNDIPFDPSKPTIAMCGTGVSGVIIKTALEQSGLAKEPIKLYDGSWTEWVGRLDESKGEVQSLIATGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7U0</accession>
    <name>A0A1E5R7U0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MIOREX complex component 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3242</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7U0">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7U0"/>
    <dbReference type="InParanoid" id="A0A1E5R7U0"/>
    <dbReference type="OrthoDB" id="5580261at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018811">
        <property type="entry name" value="MRX11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28002">
        <property type="entry name" value="MIOREX COMPLEX COMPONENT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28002:SF1">
        <property type="entry name" value="MIOREX COMPLEX COMPONENT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10306">
        <property type="entry name" value="FLILHELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="209" mass="24132" checksum="35E84D2B7EE039EA" modified="2017-01-18" version="1">MNFQKNTLTGLKGSILELLINSPRVKHPCQTLNIRKQIIFRSNSTVVKKPNNLEKYIAKSKFLTKLSTKPAFEPFFNNLKKSQPVPTITSFLLLHEFTAIAPLFVLWYAIFEYLDDSSTEDELTKTEKNSKFLQKIPEGMQENYEKWTNSIKKIVGKYYEGNREQEVVFSGVLAYGIVKLLYPARVLVSLYFAPXVGKSMTSLFRFFXK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7Y4</accession>
    <name>A0A1E5R7Y4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Multisubstrate pseudouridine synthase 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3416</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7Y4">
        <property type="interactions" value="1199"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7Y4"/>
    <dbReference type="InParanoid" id="A0A1E5R7Y4"/>
    <dbReference type="OrthoDB" id="447290at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02576">
        <property type="entry name" value="PseudoU_synth_ScPUS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.20:FF:000011">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.20">
        <property type="entry name" value="TruD, catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001656">
        <property type="entry name" value="PsdUridine_synth_TruD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020119">
        <property type="entry name" value="PsdUridine_synth_TruD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011760">
        <property type="entry name" value="PsdUridine_synth_TruD_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042214">
        <property type="entry name" value="TruD_catalytic"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00094">
        <property type="entry name" value="tRNA_TruD_broad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326:SF21">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE PUS7L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01142">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037016">
        <property type="entry name" value="Pseudouridin_synth_euk_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50984">
        <property type="entry name" value="TRUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01268">
        <property type="entry name" value="UPF0024"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TRUD" evidence="5">
        <location>
            <begin position="344"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ82998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="698" mass="79335" checksum="38B6E8CD3235907C" modified="2017-01-18" version="1">MSAELPIGKRELEPQSNGTEKHDAXKKVKIDELVKKTNITERDVGITEFLSSDLXGFCGQIKQRYSDFLVNEIDQSDNVVHLTDTGFQMPKKQRDPKPQEAGTPALSKKDEIKISRKSFKLDDVVEKELTELLGESDVLKLVKVYTDLETMETEKSFDDKALRTKLHQLIRKAFDNSLESVTTADNHFKIANNNKRSRVSREVMIEQTKDENGVENWGYGPSKQFIHFTLYKENKETMDAINIMTRFLRVPSKMVKFAGTKDRRAVTCQRLSISKIRLDRLNALNRTLKGFCIGSYVFEDEPLGLGDLNGNEFTIVIRNASTLDSSSSLSEILQKGCESLSNNGFVNYYGMQRFGTFSISTHEVGKILLSGDFKLACQLILSEQENVLPLSIDARRIWAETKNAQEALKKMPRQCVAENAILFFLSKRTKDTSLNDYSESDYYNAIMKIPRNLRTMYVHAYQSFVWNKVVSYRIKTFGLNVIAGDLVIDDAKSQGITANVTGIAGNDEEEEEKEEEEDFEEDVATNKYIRARPVTQXEIDSKQFTIEXIVVPTPGFDVVYPSNEKLKQYYIDVMAEDKMDPFNMVRKVKDFSLAGNYRPIVCKPXDVSYKILSYSGNDETLLNTDLEILNNTIAQKNSQPFEQNKLTRFATPEEGAGDKTAAILCFKLTTSSYATMLLRELMKVETSRRGDMCEVKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R812</accession>
    <name>A0A1E5R812_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3272</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R812"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="386" mass="44345" checksum="9DA85092A074BE40" modified="2017-01-18" version="1">MVRRSARLIKQGGELQAKDAETTETKSVEPAQAVDKTNSASSLNASQNVGHQEEISNESRNAGDSATVTEEKTISPLSTDQAKSDSPKPQRQKRKYVRQKRLSTANDETLDAVNGSLKKNXTKLPKSLPKHPKSQKAPAPRKMMKEPVIKPRIGKMTTTIHKTADQRKIIRVFKRKSLFPEVKQFKEKKVFKKPRHTDFTMEEQYMHDESIDERKYEDIIKDIESLTNIKKDSCIMQCMKTLREVVVPCENQRHKLLSNNKYDQATIKDKLKPSQLSDGMDINANDFMFHEDSPKLKNYDKEXDQGLYELLTKDENTLNEEHIVTTIARLNNIDISHINTLLTNRNIPRAPNSNTEIAKRASYTNDEIRRDHEAISQKMNVKRKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R8A4</accession>
    <name>A0A1E5R8A4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Importin beta-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3371</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the importin beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R8A4"/>
    <dbReference type="FunCoup" id="A0A1E5R8A4">
        <property type="interactions" value="1087"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R8A4"/>
    <dbReference type="InParanoid" id="A0A1E5R8A4"/>
    <dbReference type="OrthoDB" id="361693at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058669">
        <property type="entry name" value="TPR_IPO7/11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF7">
        <property type="entry name" value="IMPORTIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25758">
        <property type="entry name" value="TPR_IPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="5">
        <location>
            <begin position="31"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="6">
        <location>
            <position position="757"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ83124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1059" mass="123973" checksum="D37E833CAE240F8E" modified="2017-01-18" version="1">MSQDLYLDEISLIRVLEQATNQADAQNSKLAEEQLKKWETQPNFHFYLQSIYLDLSLPLNVRWVAIIQFKNGLEKYWRLTRTNAIPAEEKQQIRSRLFDMLDEQNNQLCIQNAQAVSRIARIDFPQDWPELLEFLHVQLSNEQVWKDTVKIYNLLIITNQVVKLLSMAKIGKCRPAYQKQSILIFPLLAQIYLHYFEQWTSSTNFDELDQEEVLSSMHISYLACKILRRLCCDGFQKSYQVPEVVEFMKISSQHFNLLVSKHDQFNLHHDQYEKFIVCYLKLWDKMNGVIGLGSSQFLFFPSAMGITTSLIQILLTNAEKVYNEQDDTDSFWEQLAIRSFSILKKILNYINSNKNSKTIVLKHREDTETIRHVVSEYQTNIFNNEFMSTFVDLLINYYMKLRPIELENWAMDPEEWVNEELDSSYQYQIRPCAENFFTTLTEVFKDSIPAYILNKLNEHENYDFLTKDSLFAGFQLSAYSLADHVDFDEILPRVFLSQLTGDTSTNSASEIKIIIRRICLIIGAWCEVKCSSESRLLVYQFLVQVLMSFDDKVVQLSAIQCLRTVVDDYGFVKENFEPFLKNFIDLLLYKILPNVSLTQTRLYVLKTITDVVIQTRPLIDESSLVSIMKIVPDLWASCTKDIQQSILANELLRMITNIVKSLNGKSFLTWEVSMPMVDLCCNSNPNNAELYTLLSEDGFELLNEILDNYPSEETNLDYFQQGLAKISNFLKYLMEGIENRTEILPTLLEIYKNYAILLPVDVFVSTVEFETVSSLMAKNLFKLRDDSYDIVLKIWDVLLLKVSTTSTDEQNVSAGLSIIEWFYKTGILNVITTTVFLEESLSSYQLQQLFSILSRINYINPEKFFSFLVTYHQLATLPQVENAHKEIIKRSLIYQEMSFDQVFDKFVFVWVESLRTIYNPKEKKLQILSISKIFQLLGAPFFVNSPNEKVMLLQNFNLIGGLWSEFMEEIRENESGDCTKYHEYDEYEDEYIVHKSMNYVRLQKLDKFNNPCFQVNFKKFILDVMTGIKRDLSEQEYQQFEASFMDPVIKENIQLFLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R8A5</accession>
    <name>A0A1E5R8A5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">MAP kinase kinase MKK1/SSP32</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3398</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R8A5">
        <property type="interactions" value="642"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R8A5"/>
    <dbReference type="InParanoid" id="A0A1E5R8A5"/>
    <dbReference type="OrthoDB" id="10252354at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000165">
        <property type="term" value="P:MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000263">
        <property type="entry name" value="MAP kinase skh1/pek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050915">
        <property type="entry name" value="MAP_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47448">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE DSOR1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47448:SF5">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINAE MKK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="268"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="13"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="65"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="105"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="154"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="175"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ83142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="593" mass="64819" checksum="74F71155995FA990" modified="2017-01-18" version="1">MASIFKVPDPYGNVTTPANRRKQQQQSSLKIQPTAPKTPSLQTLGPSSINAATNKENSDPLSYKPNASSSIFSNPTAPFSKTPSANTAIASSDRPKLSLPLSLQNNGTNNXSSAGGSSAGMWTPGPGSTCSVGLTPKSVSPRGRPVPPPLIAPTLTGHSSSGTLSSVTMPPGEDYSQTSTASSATPVVGANTVSSLSTKTNEKQRNDETSELLQDFEQKMSLSNGSQHSSKSQSASAIGGSLGDTLVFDIDTMDESHWHDPKYMKSQIVTLNSLGEGASGAVTKCKLKNGTKIFALKTITNNTKANKKQRQQKEQSNKFLFNNAIPEDSDDFDADTMDMETKQILRELKFNMKCSSSPHIVTMYGMFYDSSFIYIAMEYMGGKSLDAIYKQLLSKGGRVSEKVLGKIAESGLRGLFYLHERKIIHRDIKPSNILMNMKGEIKLCDFGVSGDTVNSVATTFTGTSFYMAPERIQGQPYSVTSDVWSLGLTLLEVAEAHFPLFPXDSNGNTAPNQNDEMPPIELLMMILNFKPELKDEPEYNLIWSDSFKNFIDYCLKKESTDRPSPRQMLGHPWIKGQMKKKLNMEKFISKCWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R8X8</accession>
    <name>A0A1E5R8X8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 11">Alkaline phosphatase</fullName>
            <ecNumber evidence="2 11">3.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g3252</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Binds 1 Mg(2+) ion.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the alkaline phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.1" evidence="2 11"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R8X8">
        <property type="interactions" value="198"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R8X8"/>
    <dbReference type="InParanoid" id="A0A1E5R8X8"/>
    <dbReference type="OrthoDB" id="7392499at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019637">
        <property type="term" value="P:organophosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16012">
        <property type="entry name" value="ALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000063">
        <property type="entry name" value="Alkaline phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001952">
        <property type="entry name" value="Alkaline_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018299">
        <property type="entry name" value="Alkaline_phosphatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596:SF5">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00245">
        <property type="entry name" value="Alk_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00113">
        <property type="entry name" value="ALKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00098">
        <property type="entry name" value="alkPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00123">
        <property type="entry name" value="ALKALINE_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 9 11" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="8">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ82983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="571" mass="64544" checksum="87D399E46D1D861D" modified="2017-01-18" version="1">MSASSDHRGETAPLISGGSPASPQTPMQKLKSKFQKLKNDLRWQKTMKLVLVAILLVVLIFQLLAPNTHFWPHLRKKKRNVIFFVTDGMGPASLSMTRSWRQFHFNLTSDDTLTLDKHFIGSSRTRSSSSLVTDSAAGATAFSCALKSYNGAIGVDPWQNPCGTVLESAKLHGFLTGLVVTTRITDATPAAFSSHVDYRSMEDQIAEQQLGFYPLGRMVDLMIGGGRAHYNSRADGRNLLEEAESELGWNVITNRSEFDHLEASNNRTLPLLALLADYDIPYQLDRDPKVYPSLKEEALLAIDHLHKNSEKGFFLMVEGSRIDHAGHSNDPSAQVREVLAFDEMFAAVVQWAKEHPDEETILFSTSDHETGGLTVSRQITKGYPDYVWYPEILDKCKHSGEYMIKELYKRFNYYNTMHSENIDXSGKNTKFTNFVQETVFEDWMGLSRDDYLSSDIADVFEYCENNNMGQLQDKLNDMISVRAQIGWSTHGHSAVDVXTYMYANNKELYSWALDKLQGNRENTEFGRVVEEFLGLDIDEVTELVKKTNHGILTEYGAETIQKGHDEYGHVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R997</accession>
    <name>A0A1E5R997_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein involved in COPII vesicle formation</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3335</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R997">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R997"/>
    <dbReference type="InParanoid" id="A0A1E5R997"/>
    <dbReference type="OrthoDB" id="48509at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003169">
        <property type="entry name" value="GYF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035445">
        <property type="entry name" value="GYF-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02213">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00444">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55277">
        <property type="entry name" value="GYF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50829">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GYF" evidence="3">
        <location>
            <begin position="264"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="141"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="217"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="681"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="963"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="636"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="823"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="43"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="82"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="145"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="162"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="223"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="681"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="695"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="714"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="731"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="963"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="975"/>
            <end position="984"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ83113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1099" mass="122737" checksum="FAFDE6501FBD53BF" modified="2017-01-18" version="1">MNSNFNTSNDFTKNPQPFHGQLPLNGNSPFLDHSGMSTPVARQPSNFFSPQSQAPPQGQFPQLPVNQQPAFSGANMNHSGSNFSGNISTNHNNNRNSGLPIPSSLASFSPAVPSPVPSHTPVSVPPIDGFYNRWTQPAAGPAVQPMQQHYRSNPGTPLYMPQQLNPTANFQASQQQQQQGVSGTRTPSLFFPQEQNEADTNVSTSFKNLTITASHDEPFKNNKVAQTNDSNVGNFPYKQPLGSHSGPPQPPPASTSSFPQKPVFSQWKYIDTQENWQGPFSSANMDNWLRGGFFMESLQVMREVDGNQGHVIDQPILPFGIKNRSIFYLGQLTSQYNNHLIQSGQHYSIPPTSTMYSPFQHFDTVCSQIAAVVSSHPPPPGIMSPXNDATIVNPLPTPPSSSQNQNSILHPQHTDSVVQSYNDTQKKEVNLNSRNDRGSLLSPSLMGTSMSESALQYASGTQTPVLGNKATRXFQSSFSPNDRTKTGSPRLNNKLAQKAALNTKKNGQQETTPEEIPRTKIIQSQHSPPEFSIQYILNTPSDKINTKENLAEREEDLNYQEIMHLYDPKQKSYYIESSLQIPFQKGLKEEYNEWPLDFYEMDDRLEDLAESIKKLKIEDEEIFKKYQTKKVDMMKFQAASKRKEQEEKQKKGQEELRRLKKEQEELKKKEMKDKQVEKIVQELIEMSEKDKSKKSANSNSASNNNGANNSTGSKKSKKSKKNAKKEKEATSSKTESPVSSTXPAVPPVSSSPSPSLKTQKVEPEKNKVPKQTGPLAQYKITATKGEQPKEEVAKVQPVFLAPWANAQKPNINKVSSLADVLAAEMLKKKEKDMQKEKQRASLLQKKILEAKKEEELLAKKQSGAPWASLEFQSSKKKELESLKNGIDKELQLQKQLEQSDFVKEQAKAWEELKKAQKAAAQEDSNWTTVEAKKVATEAKKQINIHTTANSYTPDTLRSVAKKSNTTASNKKTSXGNHISIPNLQQQTNVQSPKKSFLAWCKNEMKLNSGIKFADIIEVLLSLPDSPDGCEIIADTINSNSNTMNGKRFGLLFLTKRRDAENYSKDFLSWSEALKLFQNGDDVDWDFQVVSKKKKNGTKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA43</accession>
    <name>A0A1E5RA43_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Thiamine transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3012</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RA43"/>
    <dbReference type="FunCoup" id="A0A1E5RA43">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RA43"/>
    <dbReference type="OrthoDB" id="2018619at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012681">
        <property type="entry name" value="NCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045225">
        <property type="entry name" value="Uracil/uridine/allantoin_perm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00800">
        <property type="entry name" value="ncs1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618">
        <property type="entry name" value="NCS1 FAMILY PURINE/PYRIMIDINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618:SF15">
        <property type="entry name" value="NICOTINAMIDE RIBOSIDE TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="168"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="267"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="393"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="446"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="476"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="541"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="559"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="595"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ83779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="605" mass="67219" checksum="43F985CE39CCBF4A" modified="2017-01-18" version="1">MQSFFKFLEIPHDKRTTISVLRNPDLLPIPTEHQTWGFFSNFAYWGLLSFNIGTYSAASAASGIGMSYANIILSYFVGDCLTLCFTIANSFQGLNYKVGYTLTSRFVFGIYGSGLGILIRILMSIVNYSCNAFMGAYTVNLMIESIFPQYLNMKNTLDHNVAMNTRELIGFIIFHGVCVVAYFFKPYKMNYLLIWSSVASMASFVGLCIYLTHLAGGVGDGFKGSSQTIHGATFAQQFVYMASYWFGSVSPGTTNQSDYSRFAKKPWAMSLGIALGLLIPTNVVPIMGVVGTSAALKAFPGTTADDVWMPNQMCELILENTYTKGARAGVFFCGLSWTASQLAYNISSCGVPAGFDASGIAPRYINVFRGAMACALLSWACQPWNFYNSSSTFLTVMSAFGVIMTPILSIMICDNFCIRRQRYAVSEGFKVKGEFYFTKGVNWRAMFSWAAATAPGLPGLYYQIHPDQKHSPGIDNFFYANSFVAFLISFFSYWILCLIWPLKITIKQDSKDYYNAFTEAQALKKGMIPYSQLTEEDLNEVGLSKGEDSASTVTESDSETSKRESMVYGKDEETDVKKSDLETEESPESKKKSGIFSSTGFKWFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA87</accession>
    <name>A0A1E5RA87_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ribonuclease Z</fullName>
            <ecNumber evidence="4">3.1.26.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2553</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.</text>
            <dbReference type="EC" id="3.1.26.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase Z family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.11" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA87">
        <property type="interactions" value="1220"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA87"/>
    <dbReference type="InParanoid" id="A0A1E5RA87"/>
    <dbReference type="OrthoDB" id="527344at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042781">
        <property type="term" value="F:3'-tRNA processing endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990180">
        <property type="term" value="P:mitochondrial tRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07718">
        <property type="entry name" value="RNaseZ_ELAC1_ELAC2-C-term-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000065">
        <property type="entry name" value="Ribonuclease Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047151">
        <property type="entry name" value="RNZ2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027794">
        <property type="entry name" value="tRNase_Z_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553:SF49">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13691">
        <property type="entry name" value="Lactamase_B_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNase Z endonuclease" evidence="11">
        <location>
            <begin position="7"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="12">
        <location>
            <position position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ83812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="836" mass="96414" checksum="6E53B3C05A8C22B5" modified="2017-01-18" version="1">MFELIHTSHPTLDLQYPSLTXRSSAGKRYVFGKMGEGLQRVFAENHYKLNQLQSFYLTGEMNWESIGGLPGVILTAADQGKTDLNIIYPSKIVNYLISSWRYFIFRFGMSIKTFDECKPQREENFTVTPIVVELVDDLENPSASSTISPSKITEQDNSRLKRLVSKMFPVTDKAKGMKDPSMDTNFNVELPQTATLHKQTVCYEIKFDQIRGRFDAAKAKELNIPNGKIRAEIARGKEVTLESGTIVKPEQVMGPTRSYLDVLVVDIPSNDYIPKLIEAFKNSDLNKIGFVYYFLDESVLINQELCDFMNLFNREHINHYVSHSKICTNDLTYIGAALSTIKLKLINKEVYNLPIENRVYSQDFYTTFDKKLPEFDEDSTLVQENETLPLKTLLPSDNVHVFQQKDTILLEPYIRDLETPKFAFSKASRETRRLNKLFTGLYPLLPSALRGKTPENFKDALGSEILKNNFNTKGKEKDVEIITFGTGSALPGKYRNVISTLIKVPYVDETTGQIENRNVLLDAGENTLGFIHRHFNSYDVKKMFKDLKMIYLSHLHADHHLGIISLLKEWYKHNKDDPEARIWFVXPWQYNRFIREWVGVENKDILNKMKYISCEHLIDWRFVRKESLPNSFESLDDAPATKKIKLDDQSSYRDWDTINELCESLKIAEFKTCKAIHCDWAYSSAITFRKSNVPEDVFKVAYSGDTRPNLKQFALKTGYKSDLLIHEATFEDILQQDAYDKRHSTISEVISVANQMKAKKVLFTHFSQRYPHLPNLKAIESNLDGEYCFAFDGTIINWNNFNHQSKTKDVLLRGFLPEESELENLFSESSDQEETP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA89</accession>
    <name>A0A1E5RA89_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2556</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RA89"/>
    <dbReference type="OrthoDB" id="3973005at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="389"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="477"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="524"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="705"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="296"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="333"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="403"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="416"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="429"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="488"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="534"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="569"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="595"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="707"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="911" mass="99260" checksum="D3FC409199381633" modified="2017-01-18" version="1">MASSSKSPSLKSGEDKKNGGLTVVSPINNSVSGSLKNKSAIANSGHSNKMASPSQMVLPTPPKRRRGRPRILKDYVDPMKSPMANTSLKVQKQGYQEAKPFMKVGGNNSSTGSSTAVNNSTNITITNTNTNFNTAANQGDNSPSVKTTLNRLVSGTKNSGNPEVISRRSSATVISPFTTPQRFNYYMSSSQLQNVHNSADSHVSASFSETPNDYSLKPSLMTKTSPRKKKNSPNKIGKSGGSASSPLKMLKQMNTFKSNGTELIISPSTSSNYAQFQPMNGLKSKNGGSTKAPFLNGSGANGKSNSGNRKKSNSLSQSRSKLKNYGLPNIMESSSTSSSSASPLLHANRHSHSASALYETDSNKSNFTFKLAIDSTGKAFITSPLETRFPMAGTSHNQQHSKSNNNNNNNNNSNNETPSKLSNAQPPCFSSSFSSNSSSNSNFHASATRTEQNNIHEEHAXYNDTSLENIDLSLQNEHSKSDVDTDSAAASQATPLQKISTRSQADKSDIMNNKNKVLSLMNKMKKRKPKMKSTETSYASPFVSSPKGIDHKDIPTASASSHINNPIMPHEKFVEERLPKDSESQDSLDPDATEQKISTPPSFSHINDNDIEESNKHKNNYKHSGNEDDVQCSPKDYETKFTENDFNKTPMFCKDWNAHQSLLFTNGSVSTHKNFAMLGGLGDENGIGNGSPRANMFHSVNIEHANPNDNNINGKPTYKNNDTNDESHANNHLMDDEEYKKFLNSSPLGPSTLNHAPSNMMAFNLDHLLASPTTNSLLNAFSHAVHSSPLAMNRLKSTPSKGQSNFYANNIFHNQPQHQQQQQQQNQKPLHNSLDLQVLDNQIRNHSDMVPAAQQDASTEAHSPMSSAISAAHATNKKILATNTRNGTDNTSNNALYDIEFMSPSHLKRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAJ5</accession>
    <name>A0A1E5RAJ5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ADP-ribosylation factor-binding protein GGA2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2715</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">May play a role in the regulation of membrane traffic through the trans-Golgi network.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAJ5">
        <property type="interactions" value="560"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAJ5"/>
    <dbReference type="InParanoid" id="A0A1E5RAJ5"/>
    <dbReference type="OrthoDB" id="2018246at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14235">
        <property type="entry name" value="GAT_GGA_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16998">
        <property type="entry name" value="VHS_GGA_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000008">
        <property type="entry name" value="VHS domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000024">
        <property type="entry name" value="VHS domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052653">
        <property type="entry name" value="ARF-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008152">
        <property type="entry name" value="Clathrin_a/b/g-adaptin_app_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008153">
        <property type="entry name" value="GAE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004152">
        <property type="entry name" value="GAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038425">
        <property type="entry name" value="GAT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041198">
        <property type="entry name" value="GGA_N-GAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47180">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-BINDING PROTEIN GGA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47180:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-BINDING PROTEIN GGA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02883">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03127">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18308">
        <property type="entry name" value="GGA_N-GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00809">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50180">
        <property type="entry name" value="GAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50909">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VHS" evidence="8">
        <location>
            <begin position="34"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="GAT" evidence="10">
        <location>
            <begin position="189"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="GAE" evidence="9">
        <location>
            <begin position="460"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="223"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00053552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ83929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="579" mass="63057" checksum="C5013B9D9F517CBB" modified="2017-01-18" version="1">MSGIMLTDQPVRRPRPVGSPLVRKIQRACRAVVPDLGLNLDVADYVNDKQGAAPRDAVLLIVKIINHGNPHSAVCALNLLDVLVKNCGYPLHLQISRKEFLNELVKGFPERPPLRFSKVEKMILTAIEEWYQTICKHSTYKEDMGYIRDMHRLLKYKGYTFPHIKEEDLSVLRPSNHLKTASEIQKEQEIAQAAKLEELIRRGRPEDLREANKLMKVMAGFKEDNAIQAKNAIKEELTKLQRKADLFKEMLASPELDVSNETVQELYSALKVSQPKFQKIIEEEQDDQELVTQLLSFNDIVNQLIQQFFALKGGDSFEAQQLHAPADGNTAQGNDLLDLLDFDDNKXAAGPNGESTAAQQTAENDLLGDLLGDLGDLNLSSSSPSGFGAGGSISLNASSPPPQAPASAGASANSNAFDLLGDFSSTGSAPAQTFQPAATPSNTLDLLNGFQSTSSSNTSNAANVSTVLISESSHLKIEFAVSRQGTSTVKVHSLFSNVGTSPISDFTFMIAVPKTYELKLESQSSSFMSAGQKNGITQVAFIENVPSTLSKPLKVKWKCNYSVNGVAVEETAVAVLPTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAN1</accession>
    <name>A0A1E5RAN1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hyaluronan/mRNA-binding protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2581</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAN1"/>
    <dbReference type="InParanoid" id="A0A1E5RAN1"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="17267" checksum="BA78A5E6CCD9DA6B" modified="2017-01-18" version="1">MSRTTNHKINEKTKNKKNGNSNSNVNSNVNVNGVPQKHAHYFQHNGYYGTNPNKIKKNGGGKGNWGTITSEIYANESSIDDLDQDDLEVNGFHDDDNDDATTSYDYSGGNRRGLSNNKKTKHSAVLYNKSNRRNSNHRLDEERLYDLEKYQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAR1</accession>
    <name>A0A1E5RAR1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">NADH-cytochrome b5 reductase</fullName>
            <ecNumber evidence="19">1.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">AWRI3579_g2753</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">NADH-dependent reductase for DPH3 and cytochrome b5. Required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2. DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase, predominantly CBR1. By reducing DPH3, also involved in the formation of the tRNA wobble base modification mcm5s 2U (5-methoxycarbonylmethyl-2-thiouridine), mediated by the elongator complex. The cytochrome b5/NADH cytochrome b5 reductase electron transfer system supports the catalytic activity of several sterol biosynthetic enzymes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>2 Fe(3+)-[Dph3] + NADH = 2 Fe(2+)-[Dph3] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:71231"/>
            <dbReference type="Rhea" id="RHEA-COMP:18002"/>
            <dbReference type="Rhea" id="RHEA-COMP:18003"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83228"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:71232"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 19">
            <text>2 Fe(III)-[cytochrome b5] + NADH = 2 Fe(II)-[cytochrome b5] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46680"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18 19">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Monomer. Component of the 2-(3-amino-3-carboxypropyl)histidine synthase complex composed of DPH1, DPH2, DPH3 and a NADH-dependent reductase, predominantly CBR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.6.2.2" evidence="19"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAR1"/>
    <dbReference type="FunCoup" id="A0A1E5RAR1">
        <property type="interactions" value="269"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAR1"/>
    <dbReference type="InParanoid" id="A0A1E5RAR1"/>
    <dbReference type="OrthoDB" id="432685at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090524">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NADH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06183">
        <property type="entry name" value="cyt_b5_reduct_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000032">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000019">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001834">
        <property type="entry name" value="CBR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370:SF184">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00406">
        <property type="entry name" value="CYTB5RDTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 18 19" id="KW-0274">FAD</keyword>
    <keyword evidence="5 18 19" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="11 19" id="KW-0520">NAD</keyword>
    <keyword evidence="10 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="21">
        <location>
            <begin position="38"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR601834-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU361226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="OEJ83995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="289" mass="32543" checksum="B8B0799D057BE30B" modified="2017-01-18" version="1">MESNTIAITAIVLVATVALLFLQNKNSAPKFNTVLIKDQFQEFPLIEKTVLSPSTAIYKFKLPHENDKLGLPIGQHITIKHFDAENDNKEILRHYTPVSLDEDTSGYFDLLIKTYPQGKVSSMFAGLSIGDTIKVRGPKGFYKYEPNMLKHIAMVAGGTGITPMFQILKAIYNNPKDKTKVTLIFGNANEADILLRTELDQIIQSRPDQFKIYYILDKKPEVDDSWVSHIGYITEDLLREKIPSGDEPNVQLLICGPPGMVSATKRYAQALKYPRAKPVSKMEDKVFCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAZ3</accession>
    <name>A0A1E5RAZ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">glycerol-1-phosphatase</fullName>
            <ecNumber evidence="13">3.1.3.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g2738</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>sn-glycerol 1-phosphate + H2O = glycerol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:46084"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="EC" id="3.1.3.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>sn-glycerol 3-phosphate + H2O = glycerol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:66372"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="EC" id="3.1.3.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the HAD-like hydrolase superfamily. DOG/GPP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.21" evidence="13"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAZ3"/>
    <dbReference type="FunCoup" id="A0A1E5RAZ3">
        <property type="interactions" value="422"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAZ3"/>
    <dbReference type="InParanoid" id="A0A1E5RAZ3"/>
    <dbReference type="OrthoDB" id="40579at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000121">
        <property type="term" value="F:sn-glycerol 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006114">
        <property type="term" value="P:glycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006970">
        <property type="term" value="P:response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07527">
        <property type="entry name" value="HAD_ScGPP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.240:FF:000012">
        <property type="entry name" value="Glycerol-1-phosphate phosphohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000033">
        <property type="entry name" value="Sugar phosphatase YfbT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051806">
        <property type="entry name" value="HAD-like_SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43481">
        <property type="entry name" value="FRUCTOSE-1-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43481:SF4">
        <property type="entry name" value="GLYCEROL-1-PHOSPHATE PHOSPHOHYDROLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01135">
        <property type="entry name" value="C1.5.6:_HAD__Beta-PGM__Phospha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00037">
        <property type="entry name" value="glycerol-3-phosphate_phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00061496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ84067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="249" mass="27881" checksum="D1A577C7D4035107" modified="2017-01-18" version="1">MAVTPISFKVNACLFDVDGTIIISQPAIAAFWRDFGKDKPYFDAEHVIHVSHGWRTYDAIAKFAPDFADEKLVDKLEGEIPEKYGQFSIEVPGSVKLCNDLNKLPKEKWSVATSGTRDMAQKWFKVLNIKRPSSFITANDVTQGKPFPEPYLKGRNNLGYPINKEHPEKSKVVVFEDAPAGIKAGKDAGCKVIGIATTFTKEQLMELGQCDIIVKNLESISLGGYNAETDEVELIFNDYLWVSDDLQKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB42</accession>
    <name>A0A1E5RB42_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">MICOS complex subunit MIC60</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Mitofilin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g3102</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays a role in keeping cristae membranes connected to the inner boundary membrane. Also promotes protein import via the mitochondrial intermembrane space assembly (MIA) pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the MICOS complex subunit Mic60 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB42">
        <property type="interactions" value="188"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB42"/>
    <dbReference type="InParanoid" id="A0A1E5RB42"/>
    <dbReference type="OrthoDB" id="3972477at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019133">
        <property type="entry name" value="MIC60"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15415:SF7">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15415">
        <property type="entry name" value="MITOFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09731">
        <property type="entry name" value="Mitofilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ84117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="554" mass="61504" checksum="DB5579D827FD1D1B" modified="2017-01-18" version="1">MLRSRAVIQSSKRFVSTQLPAKIPQPVKQGGGFKSFVGKALLVTATTYAVGTVASHYNDQVEEMFVDHVPFGEEIVTMYDNLTELMRSKGDSTDAKTLGEKIQEMEAKKISQNNGVQAKSATLESSAKKTSDTPAIVVLEPKKNLPSLEQLNLSQFELPASQAQVPELAELVSVFNTTIDTINKSDVQTAKEAIGPIISGFKQVQDTLHKFGKFTSSTTNSEEKRAELEKEFGLKAVELQKKLTTEFIEKLNSFEKTLTEQSELLLNSSIKTNEEALKAKQANQITLLSIKQVEQFNKILKEKLDLERDGRLSKLNELNDSVKDLSQVVHKLDKYVITREASNKLMQLIGKLQKKLLTNKEITFEELNKNIANLKLLSSIIPSRMMCNCKNCKANGICECGCSRKTKLMDIAISELELAVSAPSIXKSQTLEEAQKAVLSNEQLYNRWFMISEQLQTASLLPENAGILGQFSAKIFSKLLFTKSGAVSNGESMEDVIARVGEHLKLSKLDAAVEEVTQLQGWSRIVVDDWLQSARLKLEVQTXIDVIDSDAKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB49</accession>
    <name>A0A1E5RB49_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Protein PNS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2633</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Probably involved in transport through the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Cell membrane</location>
            <topology evidence="2 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the CTL (choline transporter-like) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RB49"/>
    <dbReference type="FunCoup" id="A0A1E5RB49">
        <property type="interactions" value="232"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB49"/>
    <dbReference type="InParanoid" id="A0A1E5RB49"/>
    <dbReference type="OrthoDB" id="44736at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007603">
        <property type="entry name" value="Choline_transptr-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE (SLC FAMILY 44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385:SF4">
        <property type="entry name" value="PROTEIN PNS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04515">
        <property type="entry name" value="Choline_transpo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="231"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="276"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="335"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="433"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="469"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="10">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="10">
        <location>
            <position position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ84125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="538" mass="60319" checksum="39A5A92BBCEC144D" modified="2017-01-18" version="1">MSSPPEAHHAPMKEDDYSGNQFNDNNNNNNTNNNNDNNNNNNQENYQFRQDQYYNLDAKGDTAPIGNSFEEIFPDDKPHYNDWPFTIIFLITCCGFVAIAGLVLHGWASSSDAGSTIYNSTSTGTLNSNAAILLVFACVIALIFSYIGFITCQKWPKKFIYIGMIVNIVSGLGTAIMYLSLRYWSAGIVFLVMTCITAWCYWGMRSRIPLSVEILTTVMDVIKWYPTTLFASFLGTLVAGAFGFFFSLVIAASYMKYDPKQQNSEACAAGGGGCSYSKLIGVLVVVFFCGYYISEVIKNVLHTTIAGVYGCWYYMAKSDQGPPKRPLWGSFKRSITYCFGSICFGSLIVSLIETARSTLQLIRNTIVSDSQWATIGFFIIDLIIGFINWLAQYFNHYAYAFIACYGKNYIRSAKETYHMLREKGIDALINDNLIGIALGFYSLFVAYMSALFAYLYLRFTTPSYNSNGSYTAPLVAFSFVIALQLSNIVNETIKSGDATFFICLGNDPEVFMKSYPDRFDRIVKNYPDVLHKLSHQNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBF7</accession>
    <name>A0A1E5RBF7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein EAP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2798</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBF7">
        <property type="interactions" value="164"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBF7"/>
    <dbReference type="InParanoid" id="A0A1E5RBF7"/>
    <dbReference type="OrthoDB" id="4065296at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="482"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="547"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="185"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="341"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="482"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="547"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="557"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="584" mass="64107" checksum="4519C4BDEFFB00E7" modified="2017-01-18" version="1">MSYKKEEKELPNATELNTKIAFINESKGSQKLAKNEXSTKTENEGVNEKLEEAVDQENSSFTPFDENYKPKNLLHTPIMYSYSIKEMLDLEECSLADEANMQAVKNKLPIKSFWRLKAKPNSRDKYNNNGTQSKNQHGSHFGAFGGANFFSNDSNKQKGGRNNNDDFHSDGKTRKQKNNRRRRSQAGGNTSGSFGHSGSFGQKSDADLEEELELLKLEEEMGPSGNSMHDFEAWKLKMRQLERKKKGLPPIETPEDGALPDDSFGQSQRSHDPMAGFFNMNSPQGMASEELEPKSQDANKKSNDIKHKSSSRFTSFFSEASGSPVPVVSDQVDTPPVADSAPQNSPAVVSTSQNSKMLSFFNSKNVEQTSANPNSSEGFPDQPFITSQQQLQQQPLGPPPGIAMNTRLGGPPHSSGENSFFFEKLLSKGKYENQSPNPAAMQGGATNVFPGPNNFPQPQGKNPPIHMPGTAGMAPPIFSAHQAQQPMGQPSYQQPNGNRENPAKLHGQSRPAGNVNNMPHPNMFPHMMYGMPPPPFMQGGAPPSGFPPNGFPPNNMPFPNGFPANNIPGNGLPPFFQDQMMHPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBJ1</accession>
    <name>A0A1E5RBJ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Anaphase-promoting complex subunit CDC16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2465</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBJ1">
        <property type="interactions" value="1227"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBJ1"/>
    <dbReference type="InParanoid" id="A0A1E5RBJ1"/>
    <dbReference type="OrthoDB" id="10006270at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF9">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13176">
        <property type="entry name" value="TPR_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="463"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="497"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="565"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="606"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="640"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ84267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="758" mass="85619" checksum="2A3816DB4320F7DF" modified="2017-01-18" version="1">MSTGDPGKTPSHNRSTLPISPXVKRMHPRMGSAQKLSFQTLGASSNAANLANPGQNTSHISNLKTPSKNSFYNARLRNFSNSSTQKVDISAAGDASLTDSNGLLGNNSFAKDIKLSAAEKLRLWRHDALMQHQYITAEFIGDKVFSITKDPNDAFWLAQVYYSQGFYIKTVDFLEKNGLDATNIICQYLCGLALYQLEKYEDALNLIGETNPFKDEKEGKEPENITANFEXIDAMAKNELKSSMSGTKKNAEVSAEVLLNNKVVEFEEEEDEGIKLESSLCLLRGKIYLALNNLDMAKVSLKEACMIDVKNFESFQILVDNYYLSPDEQMKLYDSLPFQKQIVQEDEQELVKSFYCLKLSNYLSISKTGDQMETSEKEQRIDYLKKEYTLSSNADVLANEVDRLFVECNFXKCLQLCEFFVKQIDKLNFKVLVAYVSCLYELNGKNKLFQLAHRLVESCPKHAVSWYAVASYHLATNKIGEARKYFSKSAVLDPNLLCSWIGFGHTYAAEGEHEQAILVYSTSARLFPGSHLPNLFLGMQHILLDSLSLAESYFEIAVDICPYDPLLLNEMGVVYYQKGNYSKAKVYFKRACEMIERLNSITKSWVGVHVNLGHTYRKLXDPDRALRCFNQVLQISKKDDNVYTSIGLIFMKLNKLEXAIDILHKALVIQPANQIAQXLLNKAIELNMSISIADPNHPLNVNPLTQEHLRSIISEEKIKXIQYDAMEVASNIKSGSLPNIGNYSRYNNDSDDEPMDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBL5</accession>
    <name>A0A1E5RBL5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15 16">tRNA ligase</fullName>
            <ecNumber evidence="1 16">6.5.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">AWRI3579_g3111</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">One of the two proteins required for the splicing of precursor tRNA molecules containing introns. The ligation activity requires three enzymatic activities: phosphorylation of the 5' terminus of the 3' half-tRNA in the presence of ATP, opening of the 2'3'-cyclic phosphodiester bond of the 5' half-tRNA leaving a 2'-phosphomonoester and ligation of the two tRNA halves in an ATP-dependent reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 16">
            <text>ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m = (ribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="14 16">Belongs to the TRL1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.3" evidence="1 16"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBL5">
        <property type="interactions" value="102"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBL5"/>
    <dbReference type="InParanoid" id="A0A1E5RBL5"/>
    <dbReference type="OrthoDB" id="276239at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051730">
        <property type="term" value="F:GTP-dependent polyribonucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003972">
        <property type="term" value="F:RNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001934">
        <property type="entry name" value="tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019039">
        <property type="entry name" value="T4-Rnl1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012387">
        <property type="entry name" value="Trl1_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015966">
        <property type="entry name" value="tRNA_lig_kin_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015965">
        <property type="entry name" value="tRNA_lig_PDEase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32004">
        <property type="entry name" value="TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32004:SF1">
        <property type="entry name" value="TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09511">
        <property type="entry name" value="RNA_lig_T4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08302">
        <property type="entry name" value="tRNA_lig_CPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08303">
        <property type="entry name" value="tRNA_lig_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019634">
        <property type="entry name" value="tRNA_lig_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 16 21" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 16" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="T4 RNA ligase 1-like N-terminal" evidence="20">
        <location>
            <begin position="64"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA ligase kinase" evidence="19">
        <location>
            <begin position="394"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA ligase phosphodiesterase" evidence="18">
        <location>
            <begin position="566"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="active site" description="N6-AMP-lysine intermediate" evidence="17">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00055002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00073988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR019634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR019634-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF08302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF08303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF09511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OEJ84287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="799" mass="92544" checksum="834FBC3E0D2CD63E" modified="2017-01-18" version="1">MMADAVQELISQLDTASTQSDRRGKAFKTEFVIQNLSKKPVQIDSWRFNEWDYGKDHKIKLPCKARGLFTSINSEGKPYIVTRGYDKFFSIDEMPFTKWEAIEQHTTGPYEVSLKENGCIIFITGLEDGTLVVCSKHSVGVRDDVNKNHAIAGENFLRKQLGNNEDKIQALAKQLYSMNCTAVAEYCDDSFEEHVLEYSTNKAGLYLHGLNKNTKDFQTLPMSEVDEFAKVWEFQKIEFFNFEKLNTLKQFVEKCAETGCYNNQEIEGFVIRSQLKDKSQPFFFKFKFEEPYLMYRQWREVTKEYIKTRQRVFKFNRHKFITNQYLDFVIPMLEEDPQLCEDYLNGFGIIKLRKLFLTKYGMSGMEILNLGKIKELEEKNSIDYEKIDQDTKFLLVPISTIGCGKTTTSLVLCNLFPETFGHVQNDDITGKDKAQLIKRSLELLKSKKCVVVDRNNHQYLHRKQIFQWVQQHKEEYLPYDTNIQIIAVPFFDYSHIDRIFKITTQRVLDRGDNHQSIKLSSEGEKKVLGIMNGFLKHFQKLDLNKLPDSQFDNVIQGLDVTNKKNSSLLNAKIIIKALNKEYXSLVPEIPTEAEFLKAFDAAMDYKPTVVKIVKNNEKKKKAAKPVYFGLKMNKAEIMQEMVSKLLECVDXNXLRLKVERGLISEXHVTLCHTNQVKNDPESRELYEKYMEYVNKANIXSNGQNKNVKELSLHKTYSVYPKEIIWNKRIATVLVDLEDSVSCANNFPHVSLSVLDGKTKPFYSNTLAESYKLKITDEGDIKSXSLANGICLKGQLYAYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBS4</accession>
    <name>A0A1E5RBS4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g2459</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBS4"/>
    <dbReference type="InParanoid" id="A0A1E5RBS4"/>
    <dbReference type="OrthoDB" id="3972902at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13145:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCHF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13145">
        <property type="entry name" value="SSM4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="148"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="203"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="290"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="326"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="392"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="456"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="612"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="659"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="699"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="763"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="807"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="856"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="14">
        <location>
            <begin position="11"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ84347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="912" mass="105407" checksum="C8FD576D893A4F2E" modified="2017-01-18" version="1">MESEXAFLFNDSKELNRVCRICQDEENGASLYSPCLCKSYVHQTCLVNWLKAKQGVLEEGPLVYEAQCEICLTDINFKRTYNESQSKVGFFTVLNTTMKSVYPYLKLTKYIFLFCNILWLIFNTFAVRKYYLFKEFPSFTLEYIFRKSFFFIDDLSIFLQASVFVLXVLPXVWSAPLGKKIVNQYXGNPIEQKVPVPFSVSFFRLNMCMFFLAPLISTACLIVFGVIPTQAIKFLFKLQGFSNASIHLLGSFLSCFIVSLLPVEKLKHLKXSQFVLLFYTKYXAKSATVLYLRLLLSVVMMKPIIDFFFGNALFGKNNXPISIKNMTLQSLFLSPYRXFVVFTLSVMQFXNQSFFRXGSIYFAPKTNEANKTPPGIFLCSTFSLVFQTFTDIVHMLGTFTITIGAALTLLVAYKKEVAPMSFGIIIRTLDEEGFFFAAYNPQIXLLWSNMDNLFXVLGPLLSXFSVWYFKPFALVLARYFRLSSFLFGENNVSERGHLVYRRWKHKWFAPHIAQMSNPDLYTQPQFSDXVPHLFEKYSXVDAYFVPDGRFARVPNHVKTNPTKRYFVTVNKNDQVLDSKPIDVPMAKSELMDFPAYSYDVVYIPPWFRVRMFCFYFFMGVIVNITSLYMLLSSNFFGNKLLGQAVKLPXLQLLIDIDTYFTYANVFLGAALQLFLFNSFKNSKIARQEFGKITSMVRNFFIPFILXTYVVTVVQLFSXGMSFYVTTVYNIVYENLIKHETIDADPSKFYFGLPLYLLTKHSNIFLIPCGICFGISMFLVMMVIQADGTYKQXASAAWKALLKPSLKYFFRYFVIPIGSQLLFVLIQKFEFISSLQDFKQQIFLQFXTGMGTWDRCAFLMFGPVLFSVFVFNDVAPVVKKYWHRTKKTALDNLNKDKLVLENVDQKAKNSFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDN2</accession>
    <name>A0A1E5RDN2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase</fullName>
            <ecNumber evidence="4">4.1.3.44</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">tRNA wybutosine-synthesizing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g1896</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(1)-methylguanosine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + 5'-deoxyadenosine + L-methionine + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:36347"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10165"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="EC" id="4.1.3.44"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">tRNA modification; wybutosine-tRNA(Phe) biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TYW1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.44" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDN2">
        <property type="interactions" value="670"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDN2"/>
    <dbReference type="InParanoid" id="A0A1E5RDN2"/>
    <dbReference type="OrthoDB" id="271553at2759"/>
    <dbReference type="UniPathway" id="UPA00375"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102521">
        <property type="term" value="F:tRNA-4-demethylwyosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000196">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013917">
        <property type="entry name" value="tRNA_wybutosine-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034556">
        <property type="entry name" value="tRNA_wybutosine-synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930:SF0">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE TYW1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08608">
        <property type="entry name" value="Wyosine_form"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00284">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01071">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="16">
        <location>
            <begin position="178"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="17">
        <location>
            <begin position="427"/>
            <end position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00071388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ85007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="758" mass="85466" checksum="A8DF178007B0EFBB" modified="2017-01-18" version="1">MSNLEESDKSIIAKTPGSVVFTAIIVIYLVEGGRFTFALAFSTIYTLIRLEISKTESQKPKKKVCCGGKGGGCCSTKNAATGKSTQXGKKSCCGGACKSKSKADTVLNANVSSSLETESQXLGNQFDEYAIDFTNAFKNPSKKXKTTKKKQPKKIVSSKPPVTKALTISKEDLLNSQIYIFYSTLQGVSERAATTVSERLEFLHPNDLKKKPIRVHIDELDDLEDYFVSVPENAIYIMVVPSYDSDSPLDVAIDGLEEISNDFRIDKYPLTKLVGFCVFGLGDSESWPEKFCYQAKQLDVLLAKLGGRRIYPLGEYCTKTNAWGKIEEWSDLLGETLKDDQPILYQYDEAEDDNEEQEPVIDLEDIGKSEELDTTMNKKMVAKNSPTYKNLTKQGYVVVGSHSGVKICRWTKNDLRGRGSCYKQSLFNIVSSRCMELTPSLACSSKCVFCWRHGTNPVSKNWRWEVDEPEYVLEEGLKGHYSKIKQLRGVPGVIAERFQKAFQVRHCALSLVGEPIMYPHINRFVDLLHEKDISSFLVCNAQHPQQLRDMYKVTQLYVSIDAPTKTELKKVDRPLYKDFWERMLECLEILKTVQSHQRTVFRMTLVKGFNMDQVTAYADLVAKGLPCFIEVKGATFCGSSDGNGNPLTMQNIPFYEECVKFVKALAAELNSRGLAYDVAAEHAHSNCILIASKKFYINDEWYTHIDFDKFFELLNSGKSFTNMDYIAKTPEWALFGNGGFAPGNTRFYRKNRKEVTPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDS7</accession>
    <name>A0A1E5RDS7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Membrane-anchored lipid-binding protein LAM6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1958</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YSP2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDS7">
        <property type="interactions" value="250"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RDS7"/>
    <dbReference type="OrthoDB" id="3972269at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140268">
        <property type="term" value="C:endoplasmic reticulum-plasma membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120015">
        <property type="term" value="F:sterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051482">
        <property type="entry name" value="Cholesterol_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319:SF4">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16016">
        <property type="entry name" value="VASt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="961"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="6">
        <location>
            <begin position="667"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="159"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="302"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="412"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="570"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="869"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="116"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="332"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="420"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="595"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="883"/>
            <end position="892"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1055" mass="116809" checksum="FE95D326984FABF8" modified="2017-01-18" version="1">MSIKDSXDKLSDGPGLENGXEGSSLHFRDSRGSGENKDSSEHESPSSSFQDNAATDAKPLNDKSFESKEQWTSEGLVSPSHEPISVQTTPTDLHVVIPQGGSTTSSLSPVKADIDTSTSPSTTTTTMGTKTTATKGLLDTQSAGGLFNSMLASLSFATGGSGMADSSKPDLTSSEQKKKNRVSSISHRKNDSTSSYTTIFSGNGVDSPIDDKTIKAKDKQLEETIVDEPVTYYSETDTLKIPFASTKKNXDFHTTFKSVPADDRLLVDVFTCSLAKDTTNNTSSNNNLKNDGSWLQTKDKTAPIRSNSVSQKQANSAANSTFMKQNSRRDSYTSSSPTTSSYTGFSSSMSALCNGELFLSENYFSFRSNKNILSLWNTNIIIYIHDIELIDNMGKILKNKFRVNSTVLTPGEENLDKGNLTPTSSETNSANIKSSAESTNGFSNNTAVTIKTVYGKTHTFFSFNRPEIISNAIDTMWEKQINFKNSLLSTPTEKENDKLLQSNKPNNSSGLLIDTFPIDNYYVSNTRSNSNFSPSLTNVPPFRKNVFDLEDEIMSVDESVDESYDDYHKRVSGDNTFNNGLIDKNKKLEEPLQTDSEEEEEEEEEEEDDEEDEDDEEGEETDDEHVELDNSTDLEVFALKNNDAYSYSGPLFHEETSFLLSPEQEQAEYLLAEVELDAPPGIVFEILFSSTNPKFSLDFLTNQNSSNFDPAHLGNFNEKINQHGQKYREYQYDKGLNYPIGPKSTRCYVRETILSLNYEDYINVLNVTSTPDVPSGSSFNVQTRYMFRWSTGGKSVLKILYWIEWTGGSWIKSMIEKSCKSGQVEATKTFVTLLEETVAEHVTKKKMSMHEATKSLSWSKSKDSVSDEKSALLQKKKKKRTASSDLSSLQSGLSGGTDVASKVAKPTELSGRGQQSFVTAQYNQQTGFPTNTNTIGNPTGTMVGPVDGMQIMKLIISSSRILTFLLLAVLILLVLNLYFQYKIMARYNMLHDVLPDFYLNLQNLLLASSSVLPTSVSETLLNKSEASIKHDDVTQQLLNSIDQLILRRIESGKPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REB3</accession>
    <name>A0A1E5REB3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Pore and endoplasmic reticulum protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2353</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REB3">
        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5REB3"/>
    <dbReference type="OrthoDB" id="5581259at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071786">
        <property type="term" value="P:endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051645">
        <property type="entry name" value="PER33/POM33_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12703">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12703:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="118"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="313" mass="36789" checksum="2C219BBC39409D54" modified="2017-01-18" version="1">MANXSNRNGKPNGKATDMPPSNTPSSSKKQTHLYKSLQFYWFLGHALTVFHFAFYSIPFNVLYLIGPHYHQVACLLSILGTYGIVILQFFKYQKIKKNSNFDVTYNLKFFHKIIQLDNFQYFFSTLIMFLISIAFNRNNALKLIPNSVLLSPTIYSLFHCLNYICKNNSAFTYQCPPNILAYFEKFITNYNAKSVRLAQILECTTLFQFLIRLWYLPLKIVLSCISRIFNSNYSLSNSLGSIYLEFILSISYIWFFKMRLKNPDQKVKTICNNFDTQFTKFFENLQAKYNLQLVKYYRTAKYTIMTFFNAIPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REC5</accession>
    <name>A0A1E5REC5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AWRI3579_g2008</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HRD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REC5">
        <property type="interactions" value="235"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REC5"/>
    <dbReference type="InParanoid" id="A0A1E5REC5"/>
    <dbReference type="OrthoDB" id="7759664at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16479">
        <property type="entry name" value="RING-H2_synoviolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050731">
        <property type="entry name" value="HRD1_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057992">
        <property type="entry name" value="TPR_SYVN1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058051">
        <property type="entry name" value="Znf_RING_synoviolin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763:SF184">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SYNOVIOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763">
        <property type="entry name" value="RING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25563">
        <property type="entry name" value="TPR_SYVN1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="134"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="216"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="279"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="331"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="496"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="337"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="349"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="403"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="504"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="19">
        <location>
            <position position="487"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="19">
        <location>
            <position position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OEJ85234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="536" mass="61244" checksum="38C818C36136C3D3" modified="2017-01-18" version="1">MAIHVLFGELRLIETEHIFENLGFTIINGIFISSTFKDHDFFSGMLLMALNISLKVLHWILADRLDHLYHATNTQYTLSHYVFSRFHFNLALFLVIDFVLAKICFDKVSVWKAYASFFASKDHRQNPAEVPFSLVFSVSFSVMFIGLLNLCCNSLLNLYEIYRQQMITRHVSVVNGNTNNDDEGPTLDDELVDEEPFENKYRWQVSIDIAAAAMKILARLLISFPLGFPFIIIKDVVMDSLSLFNNGKKLYSILKNSRKLNTLLPDCNPEDISDKNDLCTICMDTLMDSHSXPKKLPCGHILHLNCLKHWLERSQTCPICRVNVFDQKGNLAEPVNNRGSASVSTARSTQNRTEQTIXQRQRRSENMANQPYEHADGDQMPAPTPTRPTATSENVDHQDIRAHNSNTQTQHTSKQERPNTHNQQPFQRWVPYEIRPSSRRIVFQGTNGNTHCAALKQRVRPGCARVSNCCNSENADKKKLAIPTLKDNLYASVLQTKEPTPIEDYDKEKLKRKMNELQDKLDALGGTEKNKKSRTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RED0</accession>
    <name>A0A1E5RED0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RNA polymerase II assembly factor RTP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1851</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RED0">
        <property type="interactions" value="95"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RED0"/>
    <dbReference type="OrthoDB" id="39591at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019451">
        <property type="entry name" value="Rtp1_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039600">
        <property type="entry name" value="TANGO6/Rtp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20959">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 6 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20959:SF1">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10363">
        <property type="entry name" value="RTP1_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA polymerase II assembly factor Rtp1 C-terminal" evidence="2">
        <location>
            <begin position="757"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="566"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="636"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="640"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1056" mass="121621" checksum="54CA270A8F71C0D0" modified="2017-01-18" version="1">MSAKKATIKEMKQNNDDTEQLNNNGKPRKVHDIFEKGPEFVNDTAIDRFFQDLDTNLLSKQNDVSDCDIYTAVXCKSQDDFAXYLVDKIIELNMILVQQQETTQSSPSETVTDNRELIPISLNDMRLLESLTNLFIXHSVYRYLPSGFGIPLDQRNLSKFKSDNAITINKSDNKGVLXQVLSKIMDNVFYPKTPKKQVQSVVFEMFLKGSGFSDILLGLLYLSLDDTGYRQKYEAMESLQSTYGLFEMYTTFYQSGTHPLFKQLVLEKLSTLTIRREEDGLFSLIEFVLGLRETVDNELDISKFNKVNEVVCKKPKSVSNVHYYIQIFKQIMRIFSEHLNNXAVITSVNNIIAEFYFKNKRIVVDFFFKQIYIKLFNTKHQDYSVKQTNDIFNILIGFSKQSSTDVLAALIENCNEYDFFFAIWRYCLFVFMNAEKLKLDNEYYKILVSLLKTFMELGNKYYVLNYIILNFNTNKDREWGKLIIDMETQLTRISVEGNIKDNLLNEPGTQSSELTNITALQQALVLQDFNITLLIEYLKAINNEDVTKKLFLSVMNRWCRLNTQEQDKANEEGKSIXGPKNEEAKQDEGYDNLLMLSDLKLLEKFNNEFKSDLVNEPGEILDFVDNLLKIKDLQSTTNESDIDSDDEEDECVEGDKEVDESLQTKESESNVLNIXLQLLSTMITTVENLERFSTVLISIQKNLNALYPNDLQCQTIVQRIDEEVLNRLEKKGNXSNGMKTQEQINEEILAEDRKVLKRXIFSINDPLVPIRAQGLVMLRELLLKKSSVVECKFVLELHLTQLQDKDPFIYLNVIKGLTSLCEFDSESLRHLITLYHNEDHKTLLDEVLKIGEVLINYVQLSNELFQSDLLHFLIKVCLEKINVGGIAKNGANQKNTVQDNRLRMSSMSILGMILQTNAASLLDSEISDILDCSFGILHFEGGQKLNAATYETGKVKEPKNKXNXEHGKEDTSKLMRRSAIHIIHDLLYNSGFERLPTQYNGSKLINLXHYTRQQEPDYLTCELIDQLINLIDDLVKESLTVREFDDSLISSLKIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RED7</accession>
    <name>A0A1E5RED7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Flocculation protein FLO1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1665</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RED7"/>
    <dbReference type="InParanoid" id="A0A1E5RED7"/>
    <dbReference type="OrthoDB" id="3973523at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000128">
        <property type="term" value="P:flocculation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001389">
        <property type="entry name" value="Flocculin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018871">
        <property type="entry name" value="GLEYA_adhesin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00624">
        <property type="entry name" value="Flocculin"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10528">
        <property type="entry name" value="GLEYA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PA14" evidence="2">
        <location>
            <begin position="108"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1076"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1076" mass="117266" checksum="A43D4DCEFDB4EB24" modified="2017-01-18" version="1" fragment="single">MTPTTTSIATTSTTSKTSTTSTSTTSANNGYWSGYNNPPYTKGQIGGCSVPSNAAVSLANYFKVDFFYYPWASYNTKKNTGVSDLTTYLAQAYINGGFAGTGTYSGVKTKAAVIGTGYTATNLNFAFSSISGGTETWKSVSNFQKSGKAAYIPISNFAFLATAYLVPWYSGTYTFTLSAADDLAIISVGDGIAYDCCAGSTNTGYSYEDSQINYLWTSSGMKGQNTFTVQMYMGYIYPIRIFLVNRQGASSFQFSYTAPTGDVFSDFSNFAYQIGNLPAQYCPQAVVTKTVPWTGSYTTTVVTAVSTPSTMLYNNMYTYATVGYIVENLIETPIPITSTVGYWTNSYVSTVSTTTGTSISGDTTSEWIEYYIMSPYLKTTQTFYTTGLASATTSVLTTQTVVNGATSPELIYNVYSPYALSTSVNFWDQSYVWTTSTGTTYTVSNSVTSSELVYYVASPNPRTATTTTSWYLSSSGTMTYSTSIVTVPGTGSLAAAGQHTIVTIFYVETPISRSTTAQTTYWSGSSVTTLSTSSYLVTVDSQTVFMTDYLVATPEIQETVTRFTSYTGLATTTFSTSLTTSIGTDGKATVQTFYFVETPIFRTQSSVLSYWSNTYNTTYSTNVVTTLGPYNVPTIETVYYINTPEGHATVTSTSSWTGLTISTYSTSFITAEGQDGHVTVETIYYLETPISRSSATSMTYWSGSSTSTFSITVFTSNDANNSPVISTVYFVMTPEAHSSTTLTTYWTGLTSQTFSTSYTTFTGSDNSVTVGTLYFVETPISRDVSTSFSFWPGSSNTTYSTNVMTIDLSGSPEISTVYYVATPEGHGAVTSTTDWTGTTEQTYSTDFSTFSGDDGHATVNTYYYIETPISRYTITRYSYWNNPFNSTVFSHDYTVPTQGTTIIATLYYVMTPVVNEQFTSVTYWTGESTSTYSTFLTTYSGSDSHATIDTVYYVETPMSRYISTSYSYWNGTTDSTYSTSVFTVTSNDSPLISTVYFVATPEAHATFTQTSYWTGLSTSIYDTSFSTFAGVDGYETVNTVYYIETPVSRSSSTYYSYWDGXTDSTYSTEVDTITID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REE7</accession>
    <name>A0A1E5REE7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Iron-regulated transcriptional activator AFT1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1904</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REE7">
        <property type="interactions" value="1073"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REE7"/>
    <dbReference type="InParanoid" id="A0A1E5REE7"/>
    <dbReference type="OrthoDB" id="4068596at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010106">
        <property type="term" value="P:cellular response to iron ion starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014842">
        <property type="entry name" value="AFT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08731">
        <property type="entry name" value="AFT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="418"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="559"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="748"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="427"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="570"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="594"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="748"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="761"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="793"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="857" mass="95814" checksum="42FB4CD136D7B049" modified="2017-01-18" version="1">MNYYLPNETGVPNSRSGHPVQPDPRTQGFSNGMYQPQQQQPGGGTNDFFNSVIDEIAQLRMPLDTKNSHSLPNIPYAQGRQSAPPTGLRQNTLMNFANSGSNLSTPIPQSQTSPVYDSFNNVQIRTPSFGNTQINPTMDLSPPGPIDALISLELQNFYNNNTNNYRRQSSLFMENSVSVNNMTPTAQHQGDASFLRSSSTSTMPPGTAHYGNNTPLNNHFSIQNTSSSFPSFGGQQNETYLQNSDMNLLRLSDLQLSDNGVPMVNNDIGNREVPVHAGTTVDFQDLPQQYHQDSNSHETANXSTIAVKNDEERSAKKSSTVSTPRTPLSASHSHSNYLSDLAIESLQRQRNQSKLIHLDPIPDFQDRKEIKPWLQRIFYPLGIEIVIERSDATKVIFKCKALKKADIVSDATGLQNMNSKTKEQVKESSMSPATSKNYPLPKAQPKKYKRTVSLYNQCPFRIRATLSVKRQKWNIVVVNNGHSHSLKFNSDSDDYRKFKQHLKDMEDWETVKKFDELEYRTKSNLPTNLTTIPCECGLTEEVISFNVVLPSTMTGGLLHNLKSNHHNTHSENAQSPATSLGSGVQKKKRGRKPGSTNKPKGPKSLQQQIGKRKSLETQLDTFIKVEQFLNNNEHHGGDRQDVMGLAATGLHNTPLQDQTIQQQPLLQHSMPETNTQFPEMLDSSTQFDVSRDLPVMAPYSSDNMGFEVRNQTQDFEAPVNDNLFNANEIDFTELFLKPSAVPSFQQPQLQHMPQQYPPSTKLATNKSKKSSGLPQFKHLSRNNAPSNADFHNDISSGTTEPGNTINHQDTAVKPQQSSVGDLLIKELDEMQLLDNERYLLPSPVDYNQSPATDQHDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REF6</accession>
    <name>A0A1E5REF6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1711</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REF6">
        <property type="interactions" value="182"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5REF6"/>
    <dbReference type="OrthoDB" id="4084022at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10175">
        <property type="entry name" value="MPP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="142" mass="15811" checksum="25B8F9F738E4CE41" modified="2017-01-18" version="1">MAAPVNGKLSSRVMNMKFMKKAEQVEQENEEVEKVSKLKNSAXWTLGNGFVKQSVQKPVKRPVALGATAILSMSAATPGRRVFGRAKKEPAEAPQELEAADGSKKNEDEKDLDELFKESVNKNKRQSTKQKSNKKNKKAKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REL2</accession>
    <name>A0A1E5REL2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="4 14">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g2236</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="4 14"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REL2">
        <property type="interactions" value="926"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REL2"/>
    <dbReference type="InParanoid" id="A0A1E5REL2"/>
    <dbReference type="OrthoDB" id="292747at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031502">
        <property type="term" value="C:dolichyl-phosphate-mannose-protein mannosyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23284">
        <property type="entry name" value="beta-trefoil_MIR_PMT2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000012">
        <property type="entry name" value="Protein O-mannosyl-transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF46">
        <property type="entry name" value="PROTEIN O-MANNOSYL-TRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 14 17" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="252"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="303"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="633"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="667"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="697"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="730"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="359"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="430"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="494"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ85341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="776" mass="88457" checksum="E676145E64FF7341" modified="2017-01-18" version="1">MSKVSVAQKPQKKETTKVSAVKIEKTIEKTADNAVNSDESDDELISEKDELLEKPNNNNDKLLDTVSAGDAATVAKNPLYRMEAILVPLLLTALSFFVRMYKIGASDRVVWDEAHFGKFGSYYLRHEFYHDVHPPLGKMLVGFSGYLAGYNGSWDFPSGETFPEYLDYVKMRIFNAAFSALCTPLAYFTAKAIGFSLPTVWLFAILVCFENSYATLARFILLDSMLLFFTVASFYCFVMFHNQRFRSFSRKWWKWLILTGISLGCAISVKMVGLFVITLVGIYTVLDLWTMFGDKSLKRTTYLFHWFARIVCLIMLPFAVFMFCFKIHFDLLSHSGTGDANMPSLFQASLQGTDVGVGPRDIALGSSTITLKNQHLGGALLHSHVQTYPAGSQQQQVTTYSHKDSNNHWIFQRLRGAAPWSAEIDGELEPEFIEDGGIYRLVHVNTGRNLHTHAIPAPESATAWEVSGYGDDTIGDRKDNWIIEFAEQVGDESKDQLHLLTTSFRLKNEEVGCYLAQTGKILPQWGFRQTEVACVKDPFKRDKRVWWNIETHENEKLPTPETFKYPKPNFLKSFVQLNLAMMATNNALVPDPEKDDQLASSFWEWPSLHVGIRMCGWDENHVKYFMLGSPASTWLSTVGVLAFMGLVVVYLLRWQRQFVDFADSVKLNHFLMGGIYPLLGWGLHYTPFAIMGRVTYVHHYLPALYFALIILAYWFEWXFYNTKKSNLNNILKIGIYGAYYAIVIGGFWYFSPISFGMSGPSSDYEYLNWLSTWRIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF02</accession>
    <name>A0A1E5RF02_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RF02"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="unsure residue" description="E or Q" evidence="1">
        <location>
            <position position="668"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ85489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="745" mass="84495" checksum="EB6BAED0969260C3" modified="2017-01-18" version="1">MEANQIQLSFQSDKSKQDLNTFTLSSSLNICDFLCTEGNKHAKGDKELKAPLAPQNVNFLGKSSFVSNNFRANSAFNSLTNGKEQATGSAALFXSSLLKNSSPQLTSLXHYQAQDNYRXLKNVKAXSINAGLHRKKIQELFYKSADQDKCLRSSDLCANTVCSYAQEFIGVDXDDSIILARLNRTCSXLXFDFPATICNSEEXGSINXCFLKPTNEALQNEKXFTSQNTNSLLVNPFILDETNNLRNQIKGKILINMLIDSFCKVERYENEYLQTSQNVGSTRNLNTGFNPFRDYSDVVDMIVDLMHVNDHLKRVLEDLHSLSFFIXHSKNEGTEMSKTEKQIVEGVLKKIIEILYQKYCVXAFSRFSFLKHHSHRIHYLRTIYANFAYQKIPKVAYSKQKRASWVPLCDATLDEWETPNFRINIKDKSLGTKDDIDYLCTAVEKYESIGALANSLFMKITQIVTQXYQVYSLIAKTAADFXASVTTSVKSXIDTIFQIITISVTLQNDEILLSLQNLYXMMLDSIKDGNFHELFLKHSNLQIQEYLDSVQDIDKFSRSLDSLLSNDEWGKFNQSILLVKEFQNLNDTSKKVLKSKLSDANASAHNLVACNGIYSFPGLNFVVEAKKNEKMLISKVRSTDIENTXRIVERDSAVDDQKKSGRYKKNDEVQPISDTPLQSFQSCRTQKLWFPKSLLKNSGHAFYQMINKVASLRRSTTSSGSIVIKSRTSFSMCSNSDKGLELKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF32</accession>
    <name>A0A1E5RF32_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Transcription activator of gluconeogenesis ERT1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g2019</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERT1/acuK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RF32">
        <property type="interactions" value="252"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF32"/>
    <dbReference type="InParanoid" id="A0A1E5RF32"/>
    <dbReference type="OrthoDB" id="2538135at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050335">
        <property type="entry name" value="ERT1_acuK_gluconeogen_tf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056751">
        <property type="entry name" value="PAS_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47659:SF1">
        <property type="entry name" value="TRANSCRIPTION ACTIVATOR OF GLUCONEOGENESIS ERT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47659">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24990">
        <property type="entry name" value="PAS_13"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="12">
        <location>
            <begin position="18"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="13">
        <location>
            <begin position="524"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="68"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="223"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="72"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="256"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ85183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="643" mass="72570" checksum="6418A551B4D07098" modified="2017-01-18" version="1">MTNFQDDAKRKRKNTKRACTNCAKAHATCDSERPCSRCVQKNLSDSCVDKARKKSKYLQDVPDEELAKSLRSAGSSSALQSDFQQRPQEIESAVSEQGLAQRGMXNSLDMQCVLQAQSASLPQVNHSSSQPNIRHTNFFEQQFIATPQHIIHKPLFQSSAIDSEYSSLSNILSKNTDNMLNKIPVNILYNKSSSELRASLEMSSPGSSVGSGMAPGNVYGGNFNVPHGTNQQPSQYNYANNAGSSSSSSHNSYNNFHQTNSMSSTHNLASSTMPGAQPDLSFYNNMNTQGSIYSPSYKNMTENMVRYNLEEDKYVERANICKYLGPQGAEVLSNPEINLLEQHFPLVPLDVSSGEDFQRMRFFTSPSDTQILDDEHLTLKDKRSDSNGARDGEKNTKGYQTDLVFCGLKKSKFVVFNLAKKGDPLHTCAYSLQVSPENKIYSNTRWPHSLQYDTPRDIYTKIQEPFFQTQSFVALLEHIKSRFKKEDVLTMCRDLSAIRPILIAGCIDLKEEDMIFMEHSYQRTLLQYVSFINQVGTPTIIWRRSGLISYVNDEFCVLTGWSREVLLGKMTFILEILSDECCLEYFHNFKSICYSDYKGSKSALTLTVQSPIRGKLIECVTWWTLKRDVFGLPLMIIANFLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFA2</accession>
    <name>A0A1E5RFA2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Multifunctional tryptophan biosynthesis protein</fullName>
            <ecNumber evidence="6">4.1.1.48</ecNumber>
            <ecNumber evidence="5">4.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g2293</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+) = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23476"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="EC" id="4.1.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Tetramer of two components I and two components II.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.48" evidence="6"/>
    <dbReference type="EC" id="4.1.3.27" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFA2"/>
    <dbReference type="FunCoup" id="A0A1E5RFA2">
        <property type="interactions" value="394"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFA2"/>
    <dbReference type="InParanoid" id="A0A1E5RFA2"/>
    <dbReference type="OrthoDB" id="524799at2759"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004425">
        <property type="term" value="F:indole-3-glycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00331">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000136">
        <property type="entry name" value="Multifunctional tryptophan biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000031">
        <property type="entry name" value="Multifunctional tryptophan biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050472">
        <property type="entry name" value="Anth_synth/Amidotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013798">
        <property type="entry name" value="Indole-3-glycerol_P_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001468">
        <property type="entry name" value="Indole-3-GlycerolPSynthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418:SF4">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00218">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00614">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="15">
        <location>
            <begin position="7"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Indole-3-glycerol phosphate synthase" evidence="16">
        <location>
            <begin position="221"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ85592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="488" mass="54518" checksum="D2BDEE6301B068B1" modified="2017-01-18" version="1">MSQTRVVLIDNYDSFTWNIYEYLCQEGAEVLVYRNDKITLQEIENLKPDILLISPGPGHPITDSGVSCEAIKYFIGKIPIFGVCMGEECMIQTFGGEIKYAGEIIHGKTSAVTHDGKGVFENVPQGVLATRYHSLAADLQTLPECFEVTATTTDSNVIMGIRHKKYTVEGVQFHPESILTEEGHLMIRNILNMKGFTTWEELHKQKKLTGTTINAKGPSILDTIYAKRKEDYAELSKIPGKTFEDLQSLYDHGLAPSCKNLYTTLTSNEKPHSVLAEIKRASPSKGDIDISVNAIEQGLKYAQAGASAISILTEPKWFKGSLQDLINVRKYLDLQMEQENRPCVLRKEFIFNKYQILESRLAGADTVLLIVKMLDQKLLKELYDYSRELDMEPLVEVSSKEELDRALEIQAKVIGVNNRDLHSFNVDMNTTTNLVAHIPSGTVLVALSGILKPADAETFKSNGVQGFLVGEGLMRSKNVKEFINELCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFA4</accession>
    <name>A0A1E5RFA4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Synaptobrevin 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2228</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFA4"/>
    <dbReference type="FunCoup" id="A0A1E5RFA4">
        <property type="interactions" value="329"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFA4"/>
    <dbReference type="InParanoid" id="A0A1E5RFA4"/>
    <dbReference type="OrthoDB" id="190375at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15874">
        <property type="entry name" value="R-SNARE_Snc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000004">
        <property type="entry name" value="Vesicle-associated membrane protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016444">
        <property type="entry name" value="Synaptobrevin/VAMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45701">
        <property type="entry name" value="SYNAPTOBREVIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005409">
        <property type="entry name" value="Synaptobrevin_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00417">
        <property type="entry name" value="SYNAPTOBREVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="20"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ85590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="109" mass="12252" checksum="4C86112D44294FA9" modified="2017-01-18" version="1">MSASAPYDPYIPQEDQSQNKTAELQAQIDDTVGIMRDNINKVAERGERLTAIEDKADNLAVSAQGFKRGANRVRKQMWWKDLKMKMCLVLAVIILLIVVIVPIAVHFAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFH4</accession>
    <name>A0A1E5RFH4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein MNN4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2186</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFH4">
        <property type="interactions" value="115"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFH4"/>
    <dbReference type="InParanoid" id="A0A1E5RFH4"/>
    <dbReference type="OrthoDB" id="3971919at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009644">
        <property type="entry name" value="FKTN/MNN4/W02B3.4-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007074">
        <property type="entry name" value="LicD/FKTN/FKRP_NTP_transf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407">
        <property type="entry name" value="FUKUTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407:SF28">
        <property type="entry name" value="RIBITOL-5-PHOSPHATE TRANSFERASE FKTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04991">
        <property type="entry name" value="LicD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="6">
        <location>
            <begin position="483"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="7">
        <location>
            <position position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="983" mass="114530" checksum="67ADB221EB8A9A2F" modified="2017-01-18" version="1">MQRHKAHVLVKKVILPTVFLTIVYLILHVTQTTNQIVTTDMINQYWTKWTTKSPSITAFIDSLDITPKNPDQGKVLLEYEKMLFNNEYKFSTDYTIQGNILDPAGVTSLDDVGHYDMDPRLYSSVLLQILSNNEDESNVKKVPFSWYDFTDNHDYNKLLKLNDFILDPQTEPSXGKECPIDCSFLLGSKFSMNDMKYLENLIQEPLFYYDRALYDDAFFQKKMNKYSNPVQPSDVGKYCVLHSELFSASTQQNSSSKRRHLFTPYIEITNLMDRGRPEVYALQARNYVINQLDMPLSMTMLNSFHESYQFEIDQTNNGSNFIQSQTLQKILQESPDFNFNNVHSLFDTFLSKNTPLQTNEHLAXMKTQLTKNLLVNLQDSGDDDDGDKESLWEFNATKKITELENKSSLNLHEAQYLQNLKNSITQHYTQLPKSLFEPSGIIQFDGAGRHFDSRFFNGKKFYKEKFVTQIHLNHLIKVFLKFTKANGLLTWISHGNMYGYLYNGKSFYWDNDLDVQMPISHLNLLAQYFNQTIVVEDPREGSGRYLVDVGQNLLVRHNGNGQNNIDARFIDVDSGLYIDITGLSYSSDLVTKYFFDDYKNTYDLGYXKSNTDNNDGNSQLKDPHNIVFKDPNSLYEYSDMTADEYLNYIVDRDVNDTLSQNLLSNLQQQKKNEINXLRNSKSPTKNLSPAERYSINKEMKIVNCRNRHFTPLNQLSPLISTMFHNIPALVPHQVLKVLEDEYTIPYSYAFTLYKDFIFLPQFKDWVDKNYVLDAANLSPNSKADLENPSEQEIWTKTPLEEPLDIMSLQQAENIILNLCDSDDEHKSFYVSNLINSFMQSTYRLKELELIHDSSIPREQKLQAWKDMFQQVGISKISKKNIFKDPYLFNMEYRNFLQNEKNVQNDDFSMGWSQYKKKICFKNVKELFVQMEQSEDRKLPLFITEDTDFNKMGKPLYKSGDSVYANDNLIFNAEFWNIPDGTDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFM0</accession>
    <name>A0A1E5RFM0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dTMP kinase</fullName>
            <ecNumber evidence="3">2.7.4.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2190</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thymidylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFM0">
        <property type="interactions" value="738"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFM0"/>
    <dbReference type="InParanoid" id="A0A1E5RFM0"/>
    <dbReference type="OrthoDB" id="425602at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:dTMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006227">
        <property type="term" value="P:dUDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018095">
        <property type="entry name" value="Thymidylate_kin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00041">
        <property type="entry name" value="DTMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344:SF1">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01331">
        <property type="entry name" value="THYMIDYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate kinase-like" evidence="9">
        <location>
            <begin position="10"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="227" mass="26312" checksum="AB102C889357C518" modified="2017-01-18" version="1">MDKRGKLILIEGLDRTGKSTQCDYLHKVLLPGSQLFKFPNRNTAIGKIINEYLTSGSESTSEAAKLSDQAIHLLFSSNRWELQNTIKELLLXGTDVVLDRYVYSGIAYSMAKNIPGMDYSWCFNCDKGLIKPDLTIFFHNRSVEELSSRNDYGEEKYENKEFQETVFEKFMQVFVKHEQKTYQEKNLKMLDVKGKSIEQVSQMLQNVVDKFTLEHNENGNNDFLYFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFR3</accession>
    <name>A0A1E5RFR3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2061</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFR3"/>
    <dbReference type="InParanoid" id="A0A1E5RFR3"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="574"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="637"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="685"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="785"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="407"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="574"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="599"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="637"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="685"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="704"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="790"/>
            <end position="809"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="876" mass="96253" checksum="4197C336AA655810" modified="2017-01-18" version="1">MKQKLRNQCDCHDSGNCKCHGSRKMTPSKDTISELNGVGSLADLPNNNNNNMNNNNNNNSNNPYGTTHNVENFNPAAASVTSSMRKMKRKPKRRESSSIDAKSLTNGDDMFFDDSASIISNINNININSDIGSLLGSADLRRTSSISSSMHNYGGVSKKKDLYKSSHPHNNSSMLSLDQQSVYSHSSLLSPSVDNASIHTFDHYMNHNGFHVGTPILHSSNHMNHNNHHNNYNGHGFSGSHSYNNHNGHNGMRSPSVASVHTNNNIPSTINNMNVKTNLNDNLQYLDNGDNKSIRSSFSHAITNNNVNNDININQNSENHTNDQFTVPFFNGDMTKSSSVRDTPPENMLNIDPMENKNSMAMDNANIDISTLANNGDLLFQNADWLLPQMTANSSNQMVGNLPVSNQGQAQGQAQGQAQGQAQGQAQGQGHGQQQQQQQLQQTHNNIAMASPLSNENFDLKEIAINNNTNNINKSNQQDNANSQIKFSNETPSGLFSHELDPLKLAGNYNTNMATNDINTNSSNTNNMLKISDPMAWPFQNDPSPASNTEVPTNGLLDIFSDIPDLNEIQQATLPQQQQSTQQFPQQQHTPQDQIQQPLVSPTVNTKTHSPQLNGQRKYGKPLSPPFVSAIHNSHFRNQSQNNGAHSHQHNAHMKNSPDLLQRSNSMASNLSYNSLSTMNNMGPVSSSMKQPLLSGRTSFHMGNSPSDNITTTTTNNSTSTTNSNLNSANRNSRTNMNNNNNNNNNNNNNNTGNTFINHQPHLDDAQSAKSVEVLSLTPGFVDLPEKGSKGNSSTAFQTGKSNDGLNTVTDNNMSTNFNIEPSNMTLPENFENFNLLIDPDQNSNLPVDSQQIPYTDFKPNPNDDLDILNNFTSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFT3</accession>
    <name>A0A1E5RFT3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein SUR7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2192</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFT3"/>
    <dbReference type="FunCoup" id="A0A1E5RFT3">
        <property type="interactions" value="68"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFT3"/>
    <dbReference type="InParanoid" id="A0A1E5RFT3"/>
    <dbReference type="OrthoDB" id="5419460at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032185">
        <property type="term" value="P:septin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009571">
        <property type="entry name" value="SUR7/Rim9-like_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36414">
        <property type="entry name" value="PROTEIN SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36414:SF1">
        <property type="entry name" value="PROTEIN SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06687">
        <property type="entry name" value="SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="379" mass="42505" checksum="FC3E42356C068316" modified="2017-01-18" version="1">MALLSIGNRIITVLFMMGNVLLLIFMLFSGTTDSFPINRFYWLQANTSSIPNAEFSTSTWTFWGLCAKGSNGRADCSSNQGKLSPGYPLSPYDNFGTTTNVPSDFVKNRGTYYYLSRFDFAFVWLALIFSGVNLLIYAFTFCSYSFIRVVWFLQVLATLFIMANAAVQTACVVMARNAFNHNGQNGEIGVCMMAMLWATCFMGIVLFFTTGGTFIKRAYKAHKEYVQMQKYKEDSEKYQQQVALANGPTNLGPGAPLYSNPPIVPMQSTKEPLQETEYSHQQQLHQEQPNGEGNYEFHQSYENVARPQSMIQSEHNAVLGNTTNANTTNANNTAAPSIRGTADIRSNVTHQTSNSNNKYGIKFFKVRRTQEEQTDEDSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGL1</accession>
    <name>A0A1E5RGL1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Lysine-specific permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1581</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGL1"/>
    <dbReference type="FunCoup" id="A0A1E5RGL1">
        <property type="interactions" value="184"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RGL1"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000006">
        <property type="entry name" value="General amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF19">
        <property type="entry name" value="LYSINE-SPECIFIC PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="208"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="302"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="357"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="402"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="433"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="477"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="507"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="9">
        <location>
            <begin position="72"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="571" mass="63237" checksum="FFBEFB0F399DFCF4" modified="2017-01-18" version="1">MSNNQDRVSTRGMEALSSGYESYEMKSNNFDENVKQLSSKQDYFSSSVGDEEENIEGTVSEGKVQRGLKARHMSMIALGGTIGTGLFIGISQPLANAGPVSALVAYIWMSSIVYFICQSLGEMATYIPVSSSLTVFPHRFLSPAFGWANSILYWWNWAITYAIELSVIPQIINYWSDVVPLWGWILMFWVIVTAANFAKVSIYGEVEFILSSIKVVAIVGYLIFAICIVLGAGNEGRVGFRYWKNPGVAAGIISKDPSTARFLGFVSSWVNAAFSFQGCEMVGVVAGEAKSRSSISKAFNKVFYRLVIFYIGSIFFVGMLVPYNDPRLSSDSSSAVIASSPFVISIERAGVKVLPSIFNAIVLITLISAANSDLMIGSRVLLSIAKNGNAPKILARCNKDGVPVFSVFITALFGLLAFLVVNNNANQALEWLINISSLAGCIQWFFISICHIRFIAALKYRGISRDELPFKSKGGIYGAYYSAAFLTLIIFIQGFQAFTPKFNVSDFFAAYISLIFFAVAYIGAQIYYKCKFMWAVEQIDIDSDRREIDATIWEEDKVPKNLWEKFWNQVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGL8</accession>
    <name>A0A1E5RGL8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGL8"/>
    <dbReference type="FunCoup" id="A0A1E5RGL8">
        <property type="interactions" value="83"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RGL8"/>
    <dbReference type="OrthoDB" id="4069549at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="275"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="320" mass="37480" checksum="C69E7A794192374F" modified="2017-01-18" version="1">MSFQIQYELANMKNQIQPQQLTQNVFFGPVNSLSRLDFLMENNIKHFVCVDVPTSMAVKYSEKIPLDKYAYNFVNFDSKFNKAEHTNEKLFEIMCFNQNFSSQLFNFIKQTLPSSSDLLNNEIYNMNSLRVLQSNIVTCAKGYERFEVFNDLLTIIKYAKNGNVLVVSSNGNDESLITLLISQVLRENCTADVLDAIKYVKALRPTIQELNLQQIQFFTGFIEYCEKIKSKSWNNILDYKRNANMKKMNMRTSSAYDLMQRDDDNNDEEHGSTMQLQQQQQQQQQQQQHQESFNYRPVNAMQTSNDFSRSFKRARRTDCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGV4</accession>
    <name>A0A1E5RGV4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NifU-like protein, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1313</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NifU family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGV4"/>
    <dbReference type="FunCoup" id="A0A1E5RGV4">
        <property type="interactions" value="340"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGV4"/>
    <dbReference type="InParanoid" id="A0A1E5RGV4"/>
    <dbReference type="OrthoDB" id="565552at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.130:FF:000001">
        <property type="entry name" value="NFU1 iron-sulfur cluster scaffold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.70">
        <property type="entry name" value="Scaffold protein Nfu/NifU, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014824">
        <property type="entry name" value="Nfu/NifU_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036498">
        <property type="entry name" value="Nfu/NifU_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035433">
        <property type="entry name" value="NFU1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001075">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11178">
        <property type="entry name" value="IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11178:SF1">
        <property type="entry name" value="NFU1 IRON-SULFUR CLUSTER SCAFFOLD HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08712">
        <property type="entry name" value="Nfu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01106">
        <property type="entry name" value="NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036773">
        <property type="entry name" value="HIRIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00932">
        <property type="entry name" value="Nfu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110836">
        <property type="entry name" value="Hypothetical protein SAV1430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Scaffold protein Nfu/NifU N-terminal" evidence="2">
        <location>
            <begin position="36"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="260" mass="29642" checksum="DB592B576893026A" modified="2017-01-18" version="1">MFRALLNTPKYGFCRNAKTLPFSSSFATLSRRLIHIKTVSTPNENSLKFISTDGDTFLNNNASVEIKNSDQELIKHSSLARTIFVQCPGVESIMIGKDFITINKDTQLHWRQLTNDVIDILTNHLAKGNESVDEEKYHHTVKEQEGYEINVPKFELNEEQQEVSDMIDELIQTRIRPAVQDDGGDILYRGFDPEKGIVYLKLQGACKSCSSSEDTLKTGIEGMLKHYIEEVEEVVQILDPEEEIALREFEKLESKLSEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGX1</accession>
    <name>A0A1E5RGX1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGX1">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RGX1"/>
    <dbReference type="OrthoDB" id="3972586at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="255" mass="29385" checksum="24F330109D5C4C1A" modified="2017-01-18" version="1">MQGANNITLPSQINKAEKKRKRVLSDNSNKHNRGLLPHFPDIGLLKSTDKYGYEQRPAKRLMNDSGPNPVSSVYNADMEFHGNNEPLWKXNQENFFGAKAKYSGINSHLSEIHSNNQLVLDQGHLVPLSMYSNAGYMERPGFSETIEEEYGTGSALPLHFNPRSAQNMPCEEKVVNWMEKVLIYQVADNVWETDCYSIEEYSNFEEEEFDEAQERINYNIQDLSYVCSLATPDEIIEFQCKRWSALVKKTYELDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH24</accession>
    <name>A0A1E5RH24_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Heat shock protein 70 LHS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1433</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH24">
        <property type="interactions" value="223"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH24"/>
    <dbReference type="InParanoid" id="A0A1E5RH24"/>
    <dbReference type="OrthoDB" id="10262720at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034663">
        <property type="term" value="C:endoplasmic reticulum chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10230">
        <property type="entry name" value="ASKHA_NBD_HSP70_HYOU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000029">
        <property type="entry name" value="Heat shock protein 110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF3">
        <property type="entry name" value="HYPOXIA UP-REGULATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="901"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="907"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="7">
        <location>
            <position position="813"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="926" mass="103339" checksum="C84696523617480A" modified="2017-01-18" version="1">MLLKKLTFFIAFIIPFVVGSLLGIDYGSGFSKAVVIGPHSPLEIVLTDDTKRKQVSGVGLKPLNPKNDPLDXERIYGSGTQSFFTRFPSFSALHLKSLLGKTEAPKYFEQLHPGLKIVGDRKGKXEGLQVEVGKKIYPVEELVAMDFNKIISNAESLIRSKSSTDYFTNMGVCVPAWYSQDQRESLMDVVSLSKLPNLAGLVDDGVAVGINYAIRRKQEISNTSVEYFLIYDVGQESTKATLLGISNADNEILNIEMSGYGYQAEVGGDALTAGLTDLIVDKFLEKYGKSITMKNLQKHHKAMAKIIQAAEKAKLVLSANNEATISIESLYNDDIDFRTKISRSDFESLVLAKHKAAFVQPVVDALNTSTLFVGQPLTLQNLSSIILTGGSSRVPFVQQTLLDYLGESYSGLLSKSVNADEAAVNGLGIRSIQLSNSFNFKQKINVVDRSVFDYNIVLNADKSSDDDDELHTVFPVGSNFSSVTTISVPVDKNVIGSNDLYLKLLENSVEQSVYKIKLSQSSLSNKFNVXSCDQAALNVTMRLNENRIFELLKTELVCANAENLSLDTKSSNSSLPLAKGVSTFVSFSHFKNSNKQPFTNAEIRKIKKELQEFNDRDTHRELVSKHLNNLEASIYELRSILEEHETDDLLPDNYVDSANQDVSDFLEWLDFEIDPXTSIEDIEHKLTIIVSHKKNIELYLQSINDPLDYAQFQGILANCTSYIDVMSKTIANQTEEVNDKIEXFEQYNLSAVDLYYNPDLKMFKVPAALLEKAQLFNQTFGFIRDELIVRIEELLVADGALLAEKSRXELFELKNEYSQIAEFVPYLLESAKELHASKIRYMNSLYSRRFKQSLREEEKKKLEKVKHLSLRKNGTLLEDIIGAKTSSSLDESADFVTLTDLPKDSSSKTAATPTADSSSIPEHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH60</accession>
    <name>A0A1E5RH60_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">60S ribosomal export protein NMD3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1062</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as an adapter for the XPO1/CRM1-mediated export of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the NMD3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH60">
        <property type="interactions" value="1388"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH60"/>
    <dbReference type="InParanoid" id="A0A1E5RH60"/>
    <dbReference type="OrthoDB" id="203821at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039768">
        <property type="entry name" value="Nmd3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007064">
        <property type="entry name" value="Nmd3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048899">
        <property type="entry name" value="NMD_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048898">
        <property type="entry name" value="OB_NMD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746:SF2">
        <property type="entry name" value="60S RIBOSOMAL EXPORT PROTEIN NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04981">
        <property type="entry name" value="NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21193">
        <property type="entry name" value="NMD_SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21192">
        <property type="entry name" value="OB_NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nmd3 N-terminal" evidence="9">
        <location>
            <begin position="27"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="60S ribosomal export protein NMD3 SH3" evidence="11">
        <location>
            <begin position="259"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="60S ribosomal export protein NMD3 OB-fold" evidence="10">
        <location>
            <begin position="323"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="474"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="506"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="486"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="506"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ86240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="525" mass="60187" checksum="B2D8376153073846" modified="2017-01-18" version="1">MNYTPMDLHHGHNHGSSAQPQVATVLCCNCGVPMDGSTGLVMCFDCIKMTVDITQGIPREANISFCRNCERFLQPPGQWIRAELESRELLALCLRKLKGLNKVRLVDASFIWTEPHSRRIKVKLTVQGEAMSNTIIQQTFEVEYVVVAMQCADCAKSYTTHTWRAAVQIRQKVHHKRTFLYLEQLILRHNAHVDTISIQESKDGLDFFYAQKNHAVKMLDFLNSVAPXKYKKSEELISQDTHTGSSTYKFSYSVEIVPICRDDLVVLPKRLAKSMGNISQLVICSKVTNTIQFLDPLTLQTADLSPQVFWKNXFDALADVSQLTEFIILDVEITGKVNGKKLLADVQLARSSDLGVNDQVYYTKTHLGAIIHPGDSVMGYFIANSNYNSTSFDSLNFDYVPDVILVKKLYIRKTKRNRNWKLKRMAKEHKEIDASMDYSSRQQKQEMERAERDYELFLQELEEDPEMRQTINMYKNKDKAQPNAVEIEEEEDAPQIDIDELLDELDDMTLDEPVEEEGQIEQQPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH77</accession>
    <name>A0A1E5RH77_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1333</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RH77"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="336" mass="38963" checksum="61823DFC8155022E" modified="2017-01-18" version="1">MTTSSSSSYFVPSQFPITKSASSSHHMDHDHLMNXNNNNGNNNNNTDNNDSNNNSNDNSVFSLVLDDRNHVYTNLCKILKEYAFVVVYLFTGHHSNSTTGNNTNSASRYYKKYRALHTEFLHYKHFTDPRKESWYTGGNSSGSDNRNKHISNIKNIYQMRISPNLKYFHFNYLITLCIFFLVYISYFNFQPLLFCVILFAFVYFAIVYRLIFPKPQYQTLTFNMNSKLSWSIIQHLTWIYNKVKTSVLSKKPNNHLSESTDQSFTPLHKSITLSRQQYCLVGYCICTLSILLFTGGIFNLLTHVLFNTFVVSILFILPHAILYNPVYHVTTLDQAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHU9</accession>
    <name>A0A1E5RHU9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHU9"/>
    <dbReference type="InParanoid" id="A0A1E5RHU9"/>
    <dbReference type="OrthoDB" id="3981301at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="491"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="565"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="653"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="697"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="206"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="347"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="496"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="566"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="706"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="731"/>
            <end position="749"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="768" mass="85233" checksum="6053DA9127CA31B3" modified="2017-01-18" version="1">MMPVSVVPVSHSPTLSVASTNHTITISSPPAVSNTTQTEREESTSTTSTTTTKTKTRTTTHKDESSYANDTTGAAALPMPRNRHKHKRSFAISGDFDFLKQEQDAVQRQRQEQDKYGSQNDNDFHIPSSLNTNNESNRNSKFYLSTGEETKFNDKGIPNALIDLDNVISTLNNTTLNQPTTKSSSGFVKNNNYNYNIRSKSSVVYSNTQSSSGKSSPLSSYYHQNSGFTNHRRSESAPAELDLDQPHDNNNHNFTTNFAFGYANNSNHSLLNDNHNNICEEENEDMLSNPTSPLKEQFPVRTPITPMDHSYQRKSSVPPKMANTYRNIGRQKERYSNYQAYLTSFTNSTTSNTLSTPPSTATSIASRNSTNFSSSVSSESNDAPLEVAAPGSPKNAFVDTNGENVTTFASKENLHHKLNNSATRRGTNTYQYQFTNANSKWKQRQGMPLKKSLQNTNGAKSQPFNFKTTVYDVDPQHDAFCVESSIDYNDPHLDANSGNEANRSPSPKTVLLQGNSSDDKNHNNENCNTNHTCNDNVTVASKNSDTFQKSPPPLKHMKSHSIAIGEQQSNNNSASTTPRKTTPIPNSILMGEPGSAIDLHHHASKENSPGQKRHSGDDTAESVRRNVQMGLRENIVFPAGELQTHETDVISADNLSADEGLSTQDTERTPDSKKRRRSSHIFKSAVSLIRSASSTSLAQSFNSQYKGDRTARRSFFHKDDDMDEEEKRANASVNTETNNASPGRPNTNIPKEKKKKKKLLQWLFKKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI25</accession>
    <name>A0A1E5RI25_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1489</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI25"/>
    <dbReference type="InParanoid" id="A0A1E5RI25"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="121" mass="11563" checksum="B0FDAF5EE0254640" modified="2017-01-18" version="1">MSSSATTNTNLVSSAAVAASSVTASAASVTSSLSSRISSISSSIASSTSRESSSVTSSMSSVSSSASSASSSSSASSASSSSSTKSKNGAAGSLYNTHVNWKLVTLIGGLMTFGVLAGDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI69</accession>
    <name>A0A1E5RI69_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EKC/KEOPS complex subunit GON7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g1514</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. GON7 likely plays a supporting role to the catalytic subunit KAE1 in the complex. The EKC/KEOPS complex also promotes both telomere uncapping and telomere elongation. The complex is required for efficient recruitment of transcriptional coactivators.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the EKC/KEOPS complex composed of at least BUD32, CGI121, GON7, KAE1 and PCC1; the whole complex dimerizes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GON7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI69"/>
    <dbReference type="FunCoup" id="A0A1E5RI69">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RI69"/>
    <dbReference type="OrthoDB" id="2288868at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014849">
        <property type="entry name" value="EKC/KEOPS_Gon7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08738">
        <property type="entry name" value="Gon7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0779">Telomere</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ86590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="133" mass="14612" checksum="52D0CC6B2D78737E" modified="2017-01-18" version="1">MLPKVEAQYTNENENTAKVFKITDATLSRYSTTDGKTTGPSEYVIKAGQGEDVDRPSGPKLAAYADSVTLKSGHESSIGNTTGTTEQYTFLSQVRMQLTGIQDDINEFLTEKMLESRGNKVVKQDVIQENPSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI85</accession>
    <name>A0A1E5RI85_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytokinesis protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1348</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI85"/>
    <dbReference type="InParanoid" id="A0A1E5RI85"/>
    <dbReference type="OrthoDB" id="27823at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="254"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="459"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="562"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="640"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="309"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="686"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="274"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="289"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="460"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="487"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="533"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="591"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="654"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="745" mass="85380" checksum="1C83B284CF41674B" modified="2017-01-18" version="1">MSDMTQEQMALLHHLKSKTFVNTSVVRYFQELIKLEKDHTRRINALEEKYTSVFTKTTPDSGMVQQRVILDFIQIYKKMVHKQGKLQVYMEKNIIPEFNTYQMDANVLFQRYETKMMKLIEHKHSLHKVMNTVEDKLDKVQVKVRECELNCYNQLGIKETQVLERNKNKWKLVEQELQGKLQVLEMDYKKLEKHCEMEWEEVERELKNWEMERVRKMVDVYYQYAGLKMEYDKIEIGKWDDFMSNMSEISMKEMKTNKMSTRKASEDEVSGAYTNNLSNTNRLSNTNXQGQXYXSTPQQPRVPPKGKKAKVYQESLRKLSDQLESHKGNLEQAGCNTRVSSFESVGEESRVDRTYYPHNQTIDVLQEEVDVVHRERRGVDDYHGLDFSTGEVNVTAGNVTAGNVTGVNKTIETTSPKKTHSNPSKNYTYNDSSLIEITQQDFIDETAHPDTTIINNYMYSEEEEDEEEEEEEEEGEEINAMFKNSPKRPTSNANTSVTTIETQHSCNASTISTSPSTTNEAYSPSHVHKNTEQLHNTSPSKDSNFSSSTTFNILKSIPDMEKASSSWNKKNAKKHLPPLPTREADFEDRSATTPTRSRNTSVSTTKIYSSPQRVPPVTQLAPQPVAQPVAQPVAQPXAHYPAQKKQMNPPPTMSSPSLGISSNKPLGNATASNNADPLKVAVERMRREREARKQKQLQEKEFLKQQREQVLKMVGDGVAQLEQEQADYGKVSSVTGAKLVPFTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIA3</accession>
    <name>A0A1E5RIA3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nascent polypeptide-associated complex subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Alpha-NAC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1172</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the nascent polypeptide-associated complex (NAC), a dynamic component of the ribosomal exit tunnel, protecting the emerging polypeptides from interaction with other cytoplasmic proteins to ensure appropriate nascent protein targeting. The NAC complex also promotes mitochondrial protein import by enhancing productive ribosome interactions with the outer mitochondrial membrane and blocks the inappropriate interaction of ribosomes translating non-secretory nascent polypeptides with translocation sites in the membrane of the endoplasmic reticulum. EGD2 may also be involved in transcription regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAC-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RIA3"/>
    <dbReference type="FunCoup" id="A0A1E5RIA3">
        <property type="interactions" value="576"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RIA3"/>
    <dbReference type="InParanoid" id="A0A1E5RIA3"/>
    <dbReference type="OrthoDB" id="3169036at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005854">
        <property type="term" value="C:nascent polypeptide-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22054">
        <property type="entry name" value="NAC_NACA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14358">
        <property type="entry name" value="UBA_NAC_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="entry name" value="Nascent polypeptide-associated complex domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016641">
        <property type="entry name" value="EGD2/NACA0like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044034">
        <property type="entry name" value="NAC-like_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21713">
        <property type="entry name" value="NASCENT POLYPEPTIDE ASSOCIATED COMPLEX ALPHA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19026">
        <property type="entry name" value="UBA_HYPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015901">
        <property type="entry name" value="NAC_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51151">
        <property type="entry name" value="NAC_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAC-A/B" evidence="7">
        <location>
            <begin position="7"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="89"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="95"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="107"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="160" mass="17335" checksum="997AFF87F4FDC7EF" modified="2017-01-18" version="1">MSVEIVDKNEKQAKEMIQKLGLKPVAGISRVTFRKKDNSVFSIDAPKVYRSPAGNYVVFGDCKVDNFQERMAEAQSKVQKDPASIQADLAAAAADAEKAEKSGESKIEEEDDNEPVDESGLDAADIDIIVQQANVSRGKAVKALKAHNGDIVNAIMELTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RJ40</accession>
    <name>A0A1E5RJ40_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1355</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RJ40"/>
    <dbReference type="InParanoid" id="A0A1E5RJ40"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="345"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="191"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="444"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="191"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="463"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="474"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="487"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ86573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="496" mass="55082" checksum="E94A8C63AF9CED7F" modified="2017-01-18" version="1">MNKEFSMNTNSLVQLNKYFSYDTTKTGTKDETNMINTGVNRVNYSTNESAAIGKINLNSTNSCTALLQLINEQSVYNTPTTLSNCVDNDGYLNEIGNNKFNDLIDESSDNTPSNTVFNTNDRYEHFTYGFDHFDIEENGGRNDMRKFSYYDSDSNGETANVSEDECEINTLDHSLRNEYIXNANTSQVANANTSQVTNSNTSQLNDGNGXDRAGEEDDDDGIVICPSKLMNYVDPTVLYSKLVLQKFYSKKLSETPVSEASTREANANGVDANTXASSHTAISACNHISHTPCLPPKDKEILDEILECIATPSAPTENPFTLCSSSVSSTPQRTSKKYSESERTFRCDKCPRAFFRSEHLRRHLNSVHLQLKKYQCMKCYEMGVTNKYFARSDNYKAHLKTHVCVSPATTTGTTTGEASGCCSTEQASTPFSPTSANMQVTKIHTPVASTQSPKKRGKKXKDTATQNTSTKGVQKPKRKYNKKNPIKNISSTRKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN73</accession>
    <name>A0A1E5RN73_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Prefoldin subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g649</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN73"/>
    <dbReference type="FunCoup" id="A0A1E5RN73">
        <property type="interactions" value="726"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN73"/>
    <dbReference type="InParanoid" id="A0A1E5RN73"/>
    <dbReference type="OrthoDB" id="10250441at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016661">
        <property type="entry name" value="PFDN4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21100">
        <property type="entry name" value="PREFOLDIN SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21100:SF9">
        <property type="entry name" value="PREFOLDIN SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016477">
        <property type="entry name" value="Prefoldin_subunit_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="75"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR016477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ88336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="129" mass="15307" checksum="A199E99DEACDBC74" modified="2017-01-18" version="1">MQMLPEGQKNTVQVTYDDQLKINEFSKLILQKDDVDKNLTQEKTEKEYLDDILLEIELLDEDEYMNYKIGESFLLMKQKKIVKQLNKDLTKIESKIETLENKNSDIMEKLQNLKTALYVKFGNNINLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNG3</accession>
    <name>A0A1E5RNG3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein BOP3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g838</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNG3"/>
    <dbReference type="FunCoup" id="A0A1E5RNG3">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RNG3"/>
    <dbReference type="OrthoDB" id="3972385at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021216">
        <property type="entry name" value="DUF2722"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10846">
        <property type="entry name" value="DUF2722"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="345"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="163"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="345"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="390"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="435" mass="47939" checksum="60D9AE88AC1D85BD" modified="2017-01-18" version="1">MFTPTKGNPNSAGKHPLSLLLGSNVAEWPYDNLSLCKALELRNQQEVTKQHFYKLELLKLSVQAKIPGDLIPSIFCQSTNSNGIPKNAHLLQSLSTNPQESVAQSPFKDLPDPNSDVVMINPTQKIKLQNFKFPPETSGSSSPVSGSRNNSPNGKTGSCSLFDSASTTDSSGSGNIRRSHKRTASPARIGAEAVKALNGNNTMSLTEEPESHQSPTLKRKQVQFANKGVFVNDQHSTNEKGLQLIEKPVFQKTHRRIQSLPSVLKPSPFSPELFSFKTNFKNSDTRSTLSSKDSNKNLHSANKRAHTDLKSTKVIDLDAARSTGDYQINKKFKFGFLAEGNVQAEKTNTNNENRISSVSTQETSDEDEYEGNQTNSNDSDTTIEEDCEFNDSKPFVEQETEKPIFVHEQPTVSAPESGNFVRAHKRANTLDSILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNG4</accession>
    <name>A0A1E5RNG4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphatidylinositol 4,5-bisphosphate-binding protein SLM1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g834</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNG4"/>
    <dbReference type="FunCoup" id="A0A1E5RNG4">
        <property type="interactions" value="160"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNG4"/>
    <dbReference type="InParanoid" id="A0A1E5RNG4"/>
    <dbReference type="OrthoDB" id="5598057at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13311">
        <property type="entry name" value="PH_Slm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000328">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate-binding protein SLM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046868">
        <property type="entry name" value="BAR_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046869">
        <property type="entry name" value="SLM1/RGC1-like_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043453">
        <property type="entry name" value="Slm1_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31941">
        <property type="entry name" value="CYTOSKELETAL SIGNALING PROTEIN SLM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31941:SF16">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE-BINDING PROTEIN SLM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20400">
        <property type="entry name" value="BAR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20399">
        <property type="entry name" value="PH_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="400"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="575"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="575"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="618"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="642"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="668"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ88431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="729" mass="82761" checksum="3F149EBA8EA910D7" modified="2017-01-18" version="1">MSQHPTTVSRKVVSDMASLTSDHTNANVNSNANANSNANANANANAHEASANGDTPNNNTFAQNESTAARYKEVLKNPLSLTIPTQLQPTEILAQRFASWRNIIASIIVYLKECCSIQDEIVRQHLRLSNCINFPFNNKVESATYISNVGVASSSGSSSHDKKHNAGGVGNPNSDDYIQLSRLFAPYGSGSVQDLPYVLTQYHQAMCSTAQYASRELSSILIPRLDDLRRDLLVKIKEIKDLQSDFKNSCLKEVSMTKKFLQEFQNSIEQVKYNSNYNKDPFLEKLQLNKQIKKQLHEENFLHEAFINLQTSGKELEKVVVMEIQTALQQYAKLMGQESQLVFDALIAKLDNGFIAKDPNFEWGFYVDESINKNCFIPMDLPMRQYSKIQYKNQFDPLTFEIQSGFLERRSRYLKSYSRGFYVLTANFLHEFKTGDRKKDIVPLMSLSLNDCSVEQHSKRNSQDYKFVLHAKQNGIINRTHNWVFKCDSYDTMFQWFDYLKKMTSTSDSKTRARLVTEILNAQDESKELFQKTNQQSNQQANVQLNNIPTVKINPITSPANAKINRQTIYEERQPTLSRQHSLHSSNSKQPQHTMSYNPRTSIDTMSTAGSRHRYPHHSSSVPSQAKNTGNSTSYPAQIDQELARQRTKETNGDVYSGRQDSAGRLHSMTSSTARSGITTATSNVTADDEQMGRDSLLQENQEAQVLHHHDFNHDLDLTPNTEIQEYGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNM4</accession>
    <name>A0A1E5RNM4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">37S ribosomal protein SWS2, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g780</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNM4"/>
    <dbReference type="FunCoup" id="A0A1E5RNM4">
        <property type="interactions" value="370"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNM4"/>
    <dbReference type="InParanoid" id="A0A1E5RNM4"/>
    <dbReference type="OrthoDB" id="525520at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="entry name" value="30s ribosomal protein s13, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="Rbsml_uS13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_uS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_uS13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018269">
        <property type="entry name" value="Ribosomal_uS13_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S13/40S RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US13M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002134">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="S13-like H2TH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00646">
        <property type="entry name" value="RIBOSOMAL_S13_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ88487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="122" mass="13789" checksum="E87D50C442CE2268" modified="2017-01-18" version="1">MVVYILGKPFKGKTLITYGLASKFFGVGMKTAEQVCAKLGFYPSMRMHQLNEPQVMSITKELSELTIENSLKTQILNNISLKRKIGSYAGIRHSLNLPVRGQRTRNNASTAKKLNRIDRKFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNY5</accession>
    <name>A0A1E5RNY5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g688</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNY5"/>
    <dbReference type="FunCoup" id="A0A1E5RNY5">
        <property type="interactions" value="1211"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNY5"/>
    <dbReference type="InParanoid" id="A0A1E5RNY5"/>
    <dbReference type="OrthoDB" id="1724687at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000554">
        <property type="entry name" value="Ribosomal_eS7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11278">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11278:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01251">
        <property type="entry name" value="Ribosomal_S7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ88423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="188" mass="21273" checksum="2E974B302C9D865D" modified="2017-01-18" version="1">MSAQAKILSQAPTELELQVAQAFVDLENSSPDFKADLRPLQFKSIREIEVSGGKKAIVIFVPVPSLPAYHKVQVKLTRELEKKLPDQHVIFLAERRILPKPARKSRTLQKRPRSRTLTAVHDKILEDLVFPTEIVGKRVKYMVGGNKIQKVLLNSKDLASIDYKLDSFQSVYGKLTGKNIVFEIPENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP12</accession>
    <name>A0A1E5RP12_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g932</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP12"/>
    <dbReference type="FunCoup" id="A0A1E5RP12">
        <property type="interactions" value="86"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP12"/>
    <dbReference type="InParanoid" id="A0A1E5RP12"/>
    <dbReference type="OrthoDB" id="410267at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010658">
        <property type="entry name" value="Nodulin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576:SF166">
        <property type="entry name" value="ADR278WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576">
        <property type="entry name" value="UNCHARACTERIZED NODULIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06813">
        <property type="entry name" value="Nodulin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="408"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="486"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="531"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="613"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="domain" description="Nodulin-like" evidence="7">
        <location>
            <begin position="12"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="326"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="329"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ88608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="652" mass="71618" checksum="87A75B297E347D80" modified="2017-01-18" version="1">MQLSKLQLICCFIGANLVSLGSGTIYVYSFYAPQLLQRCNIPISQSSTLSFAITIGSSAFGLLVGVIIDKVGPQIACFIGTVTTFAGYYILYLSYLHKYSYIYLLSLALVFVGLGSMSGFYSAIKCCTTNFPNHRGTAAAIPVAIYALAGVLYSTFCKWYFGENILGVFKFFMIVCSSLVFIGCLTLKVCDPKNNTEKLVSQQQQQIAAISGSRKNSTLHTNDENGRLSYDSLVRGSSFDNNTNEVNAPHISKPMSIKPKRTESAVWSKELVGSLAYWGWGKVRDSPGNGTVSRGTSFDKRNSRIGSIGSSQRVSSLMQSAEFYTNDNTHNRHDSSALDMEDAHDGNDESWGDHQGMLPLQQQQQQQEVGSKHKLSDVSNTMTLKEVEAHSSTRDIPLSSIVISKKFIGYYLILAILQGVGQMYIYSVGFLVATQLATHEGTTNLNQETVQSIQVSLIATMSFLGRLSSGPLSDFLVKKFKAQRKWCIFLAALLMAGTSVYASKNPTSTHTTEAIPDVTDMKTTPDFVKRISISSVLFGLAFGFTFGTFPATIADAFGTNGFSTIWGLATTGGLVSVKYFSSVLAHDLASHSDLDELHHDGVCRLGSKCYMHTFKLCAFFSLFAAAITLYMIFIKYRYRKARVADYRRRSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV57</accession>
    <name>A0A1E5RV57_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">UDP-N-acetylglucosamine transferase subunit ALG13</fullName>
            <ecNumber evidence="4 12">2.4.1.141</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">Asparagine-linked glycosylation protein 13</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ALG13</name>
        <name evidence="14" type="ORF">AWRI3579_g514</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 12">Involved in protein N-glycosylation. Essential for the second step of the dolichol-linked oligosaccharide pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an N-acetyl-alpha-D-glucosaminyl-diphospho-di-trans,poly-cis-dolichol + UDP-N-acetyl-alpha-D-glucosamine = an N,N'-diacetylchitobiosyl-diphospho-di-trans,poly-cis-dolichol + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23380"/>
            <dbReference type="Rhea" id="RHEA-COMP:19507"/>
            <dbReference type="Rhea" id="RHEA-COMP:19510"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57269"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58427"/>
            <dbReference type="EC" id="2.4.1.141"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 12">Heterodimer with ALG14 to form a functional enzyme.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the glycosyltransferase 28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.141" evidence="4 12"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RV57">
        <property type="interactions" value="327"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV57"/>
    <dbReference type="InParanoid" id="A0A1E5RV57"/>
    <dbReference type="OrthoDB" id="20273at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004577">
        <property type="term" value="F:N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039042">
        <property type="entry name" value="Alg13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12867">
        <property type="entry name" value="GLYCOSYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12867:SF6">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYLDIPHOSPHODOLICHOL N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 28 C-terminal" evidence="13">
        <location>
            <begin position="120"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ90837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="240" mass="26827" checksum="B499B9C17388E64E" modified="2017-01-18" version="1">MVNIYITVGATVPFDALVQQLINNLNLLITNAIQTFHDSTTNTPETVNIVFQYGNNYSDRFESLLNTYLAKQSDFIADLSSKNINPTSKTSQESVFELLETNTILHTFLHTTTIKSKVTICGFALSNNIPAFLHAYLPELFITHAGTGSLLDALRLSSPKLSSSKRVVVLCVVNNKLMDNHQLDIAQKFAMLNLVTCVESPNXLKEYLEKLADIVRDTTHDNLQMGFNAKFKDEVLLGNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV66</accession>
    <name>A0A1E5RV66_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cytochrome c oxidase chaperone, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g441</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PET100 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RV66"/>
    <dbReference type="FunCoup" id="A0A1E5RV66">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV66"/>
    <dbReference type="InParanoid" id="A0A1E5RV66"/>
    <dbReference type="OrthoDB" id="18175at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018625">
        <property type="entry name" value="Pet100"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33968">
        <property type="entry name" value="PROTEIN PET100 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33968:SF1">
        <property type="entry name" value="PROTEIN PET100 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09803">
        <property type="entry name" value="Pet100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ90784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="112" mass="13311" checksum="2770ECC458E87548" modified="2017-01-18" version="1">MFTKALKLNKVSRSQLEVFRFALCLLAPVGVMYYIGIDTHKKLHVEGFWPDPETLNKVPKERYEIEAELARMKKERLQKRLKLQERLINEFGVTDFEEEKRKILAEEAMNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVC0</accession>
    <name>A0A1E5RVC0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Choline kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g324</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the choline/ethanolamine kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVC0">
        <property type="interactions" value="366"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVC0"/>
    <dbReference type="InParanoid" id="A0A1E5RVC0"/>
    <dbReference type="OrthoDB" id="3971405at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004103">
        <property type="term" value="F:choline kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004305">
        <property type="term" value="F:ethanolamine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05157">
        <property type="entry name" value="ETNK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007521">
        <property type="entry name" value="Choline_kin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603">
        <property type="entry name" value="CHOLINE/ETHANOALAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603:SF93">
        <property type="entry name" value="RE24176P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04428">
        <property type="entry name" value="Choline_kin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01633">
        <property type="entry name" value="Choline_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Choline kinase N-terminal" evidence="3">
        <location>
            <begin position="74"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="544"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="561"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ90776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="624" mass="70449" checksum="0A892E73816A0C18" modified="2017-01-18" version="1">MSTXDLATKLQKDTAHSNSGPASLASAAKTEERTKQRKNSKPPTLRRHSSSLSFKKYHASDEESEFHEEDALLTIPFIDSSVDPSLPTKYLKQDIVSCIEQLRIPKWGSKISQNKLDIKKLKLNKISGAMTNAIFKVEYSKLPSLLLRIYGPNVDTIIDREYELKVLARLSLKDIGPKLYGCFANGRFEEFLENSTTLSRNDIRDWKTSARIARRMKELHAHVPLTKSEKQGIPISWKKVSQWLQVLQDSKWDQLEPEKFAKVFSVPTMQDLKDVVGLYEKWINENDDSELCFCHNDAQYGNLLFTSPNSDFIVHNKSANELNHENNVSAPPSSSTVSDALPAESSSTSLFPVDSNISVDSIINVPNTEXLKDNRLVVIDFEYSGPNPAALDLANHLSEWMHDYHCEHPHLVSPENYPNKEQVLNFLYSYCAGGKTRRGSFNDQKSIKNDLLSPFHKLSLNETDMNEQVVTMYNSIIKWRPMVQLFWALWGVLQSGELEAAAARAKKEDIANHNASETSANTQLKVVHGPDGQYIISEFKEQPGETNVFSSSDSDNDVDDEGKSLDQTPKDTGTDIDTFNYLEYSHGKLDIFYSDLIHLGIIDAGSILHDQXQLPSLDTEYLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVM4</accession>
    <name>A0A1E5RVM4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">V-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g376</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVM4"/>
    <dbReference type="FunCoup" id="A0A1E5RVM4">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVM4"/>
    <dbReference type="InParanoid" id="A0A1E5RVM4"/>
    <dbReference type="OrthoDB" id="190375at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd15843">
        <property type="entry name" value="R-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051097">
        <property type="entry name" value="Synaptobrevin-like_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136">
        <property type="entry name" value="SNARE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136:SF168">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="3">
        <location>
            <begin position="152"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ90981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="237" mass="27241" checksum="5F5AD860EBE0FEBF" modified="2017-01-18" version="1">MSNRAYEYHIVYYCLVRVLQDDSTTATSREIVHDYFKSPSSTQYSSIIDKNLSQQLLQKLVVNNIDFPQNQFDITLLARKYIENYQIFVRQVQHLNAYQLVISSKNTPKSLSIKLLESLNSLNSKNLYKDIENFEKNYNQEVSKQIQETVPNASKITDDMDQLITIMNDNIDKVLLRNDQLDSLVNKTNKLNNSGVNFKRKTVVIKRKMWIQKVKTKAAIGTAIVLAIGGTALYAAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVR8</accession>
    <name>A0A1E5RVR8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Chromo domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g421</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVR8">
        <property type="interactions" value="929"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVR8"/>
    <dbReference type="InParanoid" id="A0A1E5RVR8"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000123">
        <property type="term" value="C:histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17993">
        <property type="entry name" value="DEXHc_CHD1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000007">
        <property type="entry name" value="Chromodomain-helicase-DNA-binding protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000130">
        <property type="entry name" value="Chromodomain-helicase-DNA-binding protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041150">
        <property type="entry name" value="Cdh1_DBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056302">
        <property type="entry name" value="CHD1-2/Hrp3_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025260">
        <property type="entry name" value="CHD1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF14">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18196">
        <property type="entry name" value="Cdh1_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13907">
        <property type="entry name" value="CHD1-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23588">
        <property type="entry name" value="HTH_CHD1_Hrp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01176">
        <property type="entry name" value="DUF4208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Chromo" evidence="10">
        <location>
            <begin position="295"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="10">
        <location>
            <begin position="397"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="495"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="804"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1078"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1333"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1407"/>
            <end position="1456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="59"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="108"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="163"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="181"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="208"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="268"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1081"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1333"/>
            <end position="1352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1353"/>
            <end position="1371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1419"/>
            <end position="1428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ91037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1612" mass="184950" checksum="BB1B6A7EA9BF3C1E" modified="2017-01-18" version="1">MVSELPDDVLEHPDLYGLRRSGRTSANARNTHYYTSDDDDDDMDKSDKENYSDVAAAEGMDDDDDDDDDDDEIVVKKRGSTGRGAKSTAASRTSKRSASKKTNYNEQEYADLDDDDEQGYDSMDDFLAEDDDDXGYEENNKSSTKLKKSGTSMVNSHKTSGLNKKKSIKKKKNLRGDGGYATNSKQDQIPTRFSNRSKSKVVSYKVFDEDDEDEDEMEQDWLVSDDGNDNDELDGYADDDGEPQESIDCVINHRWKNDTNDDDDDDNNNNNANSNNNNNALDNLSKLSSIDQDDQIIQKLTHEPDLQECKEKFQFLIKWSGKSHLHNTWENFDTQTLSIQXKDNKKPIKGLKKVDNYCKLFIIQNYLFRNDPYTTREEIEVMDIEKERRLDEFEEYKKPERIIDSERNDHNQLQYLVKWRRLNYDEATWELASEIVQLAPLEVKHYQNRINSSILPKNSSNYNQRPKFEKLIEQPKFIKNGELRDFQLTGLNWMAFLWSKNQNGILADEMGLGKTVQTVAFISWLVFARRQYGPHLIVVPLSTLPAWQETFEKWAPDLNVIIYMGNTQSRDTIREYEFYSGSNKKSGLKFNVLITTYEYILKDRQELGSIKWQFLAVDEAHRLKNAESSLYESLFSFKINNRLLITGTPLQNNIKELDALVNFLMPGEFSIEREIDFENQDDMQEEYIRDLQKRLKPYILRRLKKDVEKSLPSKTERILRVELSDVQTEYYKNILTKNYGALTSNGAQGHFSLLNIMAELRKASNHPYLFDSVEDMVLNKFGDGQMTRENILRGLIMSSGKMVLLDQLLTRLKKDGHRVLIFSQMVRMLDILGDYLKIKNINFQRLDGTVSSQKRRVSIDHFNAPGSDDFVFLLSTRAGGLGINLMTADTVIIFDSDWNPQADLQAMARAHRIGQKNHVMVYRFVSKDTVEEEILERARKKMILEYAIISLGLQEDNKKKVKDQPVNSELKEILKFGANNMFKANDNQKKLEQLQLDDVLNHAEDHVTTAEVGESHLGGEEFLRQFEVTDYKADVDWDDIIPMEELQKIKEDEKKRKDELYVKEQMDIAQRRQQTLERMRNSNGQPKSGNSDGNQSDSEDNDSLSSSNEKKPSARRSNAMNKLADKDIKSLYRVLQKFGYLDAEKIEQLMMDRQLAPKNPNSYLKKYDELLKYAQEECIVQDKERXEILKDMEEKARVFQEKIKRGEITNEEEINASENPVKLLNAKKREKKSISIDFYCLNGVNAETLVKKNNDMKLLKNYXNRYFREDPLKFKLRKPCKTAVNWSCEWDQNDDEKLMIGIYKYGYNSWAQIRDDPFLGLTEKILLDGKTNATKESTPLPQNSPTNDNSLATTTXSSSSSSSSSSSSSSTKAAATQAALNKKNLTPQASHLNRRVDTLFNHLRDIVENPDLPTGPKAHSNSPGSTPPISHALKRSAPTTKSXSANGNNQNGNKKMKFLPLNIPIAPKALLQQQQKDQQHQQKTHSNXEEAQIKKLMNPVRTSLVKLKQSGKTTDKKERLKILKKELLKVGNHIKEQSVVAAAAXAAAAATATAAANKSNGNREDKDSLFYKLWNYAALYWPSRVESKDLIAMHKKILKSVPQLPTGPAKKX</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RWD1</accession>
    <name>A0A1E5RWD1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Protein transport protein SSS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g430</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RWD1"/>
    <dbReference type="FunCoup" id="A0A1E5RWD1">
        <property type="interactions" value="500"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RWD1"/>
    <dbReference type="InParanoid" id="A0A1E5RWD1"/>
    <dbReference type="OrthoDB" id="2401875at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.820">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00422">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023391">
        <property type="entry name" value="Prot_translocase_SecE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008158">
        <property type="entry name" value="Translocase_Sec61-g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00327">
        <property type="entry name" value="secE_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12309">
        <property type="entry name" value="SEC61 GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00584">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103456">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01067">
        <property type="entry name" value="SECE_SEC61G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ91023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="73" mass="8195" checksum="4260DC8BB676A1D5" modified="2017-01-18" version="1">MAKQSNTNNQVEKFTEVPLEFVKEGVSFINKCKKPNNAEYMKIIRAVGVGFIGIGVIGYAIKLIHIPIRYLIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RYT8</accession>
    <name>A0A1E5RYT8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Chromatin structure-remodeling complex subunit RSC9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g257</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000002">
        <property type="protein sequence ID" value="OEJ92127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RYT8">
        <property type="interactions" value="304"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RYT8"/>
    <dbReference type="InParanoid" id="A0A1E5RYT8"/>
    <dbReference type="OrthoDB" id="338531at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052406">
        <property type="entry name" value="Chromatin_Remodeling_Comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003150">
        <property type="entry name" value="DNA-bd_RFX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22970">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22970:SF14">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51526">
        <property type="entry name" value="RFX_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RFX-type winged-helix" evidence="6">
        <location>
            <begin position="592"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="701"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="30"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="708"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ92127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="804" mass="89512" checksum="C6688DAFFAB343FD" modified="2017-01-18" version="1">MTESNQQIPVEIEEPTTKAAGAGEVGGAAGRAAGETAGEVGGAESSTKPVENHFKSVLPVATKKITKSVGQNGMSSPIVINRNTIINLNNIPLYNKANFDKLDVETVLNSDYQSINTELQNAQGRYKNNGIYNQQQQQILNKKVKSYKRKRPLERELNRIVMGLKSVGKEDVMWSLRKLLQLSNATNINTSSANSATSANSANSANSAISATSAISAIDKXKNTINSSNSNNTESNSGNTNSVFTQVYPILIKMSLQSITNVNFTTPLNTKDVFIVECSLTSLLILRNLAQDLDHITNLIKLNGNVFKKMIFKYLSFTKKYLYDSNANTQLFHTNGTFVKEFQYYTIDLIEAIASFLQPTNFQDSEVDNEEDQVVYGCLVEILKMVKDKYLIISILRSLSRLNALTKTTSXNMSEEXETTGLNVNAKGINTNGVTEKITSKTVHELNVNTLSGHDPNTTSKNIIPSIKEKGSSYAVIDSPLLTKIVSFLLDRSTNDDELLLAVLDFLQQYVHQNFASPVENLFSNEQRYTLLTTVFPQLLAFKIXNXPQYLNYNMQQVKLIQRVQPKIPEQPPILPDALFNEILQLNEPERSTAYLRCCFEPDLEAEFTQIKLWRCYDSLFGKKVKENNRKMIAAVDFIKNVSNSISTASAVVFPDPVDPTKKRFVVKGIKPRPFAVGIATGTKLAMIDSETLQKMSKNFTKLSXSNDKQENKDKKSNTKTIELPQQTKLHDIKFPTKLSDVSNTTTVLLNLLSKSNNKKCQQFCLELKPMIMRKIVDIPPLAPALKDYLKLCTNGTEGPVASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RYU9</accession>
    <name>A0A1E5RYU9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Signal recognition particle SEC65 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g272</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000002">
        <property type="protein sequence ID" value="OEJ92137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RYU9">
        <property type="interactions" value="85"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RYU9"/>
    <dbReference type="InParanoid" id="A0A1E5RYU9"/>
    <dbReference type="OrthoDB" id="2190947at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006617">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.30">
        <property type="entry name" value="Signal recognition particle, SRP19-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002778">
        <property type="entry name" value="Signal_recog_particle_SRP19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036521">
        <property type="entry name" value="SRP19-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17453">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 19 KD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17453:SF0">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 19 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01922">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69695">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0733">Signal recognition particle</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ92137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="215" mass="24933" checksum="32F298C84FD4E88F" modified="2017-01-18" version="1">MALEDYEDIDEMEMSLSDIAPXVDTPSNTKLDDKYILIYPCYFDKNRTIKQGRRLKKELCIDNPLGMIVAATCHMLQYDMKYEFDKTHPQDFLNPGRIKIKKYTKSGIKKDKNQILMEISKNMPRSTQVVVKSLTTSDIVEIDSSVINKQYPGIKNNKISFLPRNSPLSEMTSSKVKMLQASYEEIVKKQEVEQKTSKTLQKKIKKGNKIKMIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZF0</accession>
    <name>A0A1E5RZF0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Meiosis-specific serine/threonine-protein kinase MEK1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g208</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHEK2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZF0">
        <property type="interactions" value="249"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZF0"/>
    <dbReference type="InParanoid" id="A0A1E5RZF0"/>
    <dbReference type="OrthoDB" id="74764at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22670">
        <property type="entry name" value="FHA_MEK1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="41"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="165"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="4">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="4">
        <location>
            <position position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ92352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="511" mass="58525" checksum="363D0691CD9B668B" modified="2017-01-18" version="1">METQEECTTLTEKETIYGYMELITKDSREIEKMPIHKAKVSKFGRSSELCDLHIEDRIISGTHCKFWGIQFDENSLPVCYVQDVSLNGTFVVGKKTKNNLSVYTHHKLVKYECYPLQNGNLIKFPTIEGFYLKFASHARYANGESNDEHVFKDLRINKCVHDEWVIQPIFVGSGTYGHVMITTAKSSGRAGAVKIIRNMSARDRSLTQDKRNSQTMREAKLLLELNHSNIIKMYSTVISNGISPSYKDYYIFQQLVFGGDLFSYLAKTDSLAPIEEEECLLIVYQILLALNYLHAQNIVHRDLKLDNILLRTPEPCTKIILADFGIAKRLTFANRLHTVIGTPEYCAPEVGFQNKRVMCKSLTKTMAKQHXKEGYDFKCDMWSLGVITYIALTGVSPFFQEAECXTESMFDKMQFGNLNFGATQWADITSEAKDFVQSLLTVDTKQRLGAEQALQHKWISQHAVILQKIYKKKILKEEPTFELQDMKTPLEPSKKKPKLQLTMVKSYSYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZU7</accession>
    <name>A0A1E5RZU7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g63</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RZU7"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="570" mass="65318" checksum="E6E01269059878D1" modified="2017-01-18" version="1">MCFKDTFGTLFDSEPDENTHYDNSTHADNXATSVKSQKRRXKKRFRXKFKSKLKDLNIRVSSVNIVENVITSLLENATTSETESVFRICLLIGEKLENVQLFNALNISSSRLVQTIMLSGPQADAFLDICFSSFCPETNDXVFFEWLPSSKVCSFLQTAQTVMNENIFWKFKKHLSTKSKNAVQNLFKNVYLERPDYPGVKEIETLCSALGAFTTHEKLALICLNFDGNMTLPYPVVEFLNNFDFYTLNSILICSFEFSXKKLCCPACYALHNVYCQKEMLDECTITTFLDFFNEYXELKHEAPDDKTVKVLREMVVKSINNXYLVKSLTNFFTQRQLGEIVIQMVDXFLEKFNKNTAASSIDELNMVLTFCSCFCNMHQDAHDLTNENFTKIQRFTRSAIIAKSGEDIAQIVFDDLLAIPLPEKNTWSYQSTEITKILNLAHRATKKVTPKGVELIQVYPLHRCVIATPVKLPGYNVVHFELDFFQYQIIELFNCSDIEDFQTIMNLFPEMWHAAVKAGLKRLIGIQLFKPILKGNNEALKLQLDKVSVPSSVFTYLLEPGQLVIKLCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZX1</accession>
    <name>A0A1E5RZX1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RHO GTPase-activating protein RGD1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g61</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZX1"/>
    <dbReference type="FunCoup" id="A0A1E5RZX1">
        <property type="interactions" value="161"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZX1"/>
    <dbReference type="InParanoid" id="A0A1E5RZX1"/>
    <dbReference type="OrthoDB" id="437889at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005933">
        <property type="term" value="C:cellular bud"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000063">
        <property type="entry name" value="Rho GTPase activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050729">
        <property type="entry name" value="Rho-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF128">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN RGD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-BAR" evidence="6">
        <location>
            <begin position="21"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="5">
        <location>
            <begin position="544"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="313"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="108"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="315"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="334"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="345"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ92512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="750" mass="83123" checksum="1036CC19216A19CE" modified="2017-01-18" version="1">MTTVSTPGSAENAVDNILNRPEIQQVLSSDIAINTLLTKLKQSILTCEEFSKYIKKKVLIEQEHAEEMSKAYKNFFSSTSNSLTKSIAELLQFDGKLSSVKTSYVIALQKMHDELNALLLTMSKARKQVKDHSRRLEKEVMEAIHQAEKAKSKYDSLCQDWSKLRMSDPTKTKLTLRGSKTTREQEEELQRKIDAADLEYKQRVDHATSLRNSFISRERPKIVVELKDMILEMDIAISIQLQKYAIWTENLMLNSGISVCPVNNTSSNTKSMKSVASSMSSEYDLYNYLKKYTTNDKNRNALVNKNLIPVEYKKNSNVGGSSSSKISKPVNGTFVMNSNGKQVPSSTATSNSSPNTFTNHGTNNSISSTTSTKPANTNPYGSSITPKTSNNYNSNRVDAVQNGVGITTNTNYPSKQLSPNKQHMLNNNNMAGVNALVGGVGASAAAAAAGSGRSFGYNATRAINGNQNTQGGSAGYGMAPVNEDAQAATYSTLDPSKSPISSVVSNFTNDNQNNLHPNDRPLSHVETNVTLPPGLNKNFKTFGVPLGTLLEFEQDLVPAVVRQCIYVIDKYGLDIEGIYRKSPSVVDVNKLKEEIDTDPANISMILPPKNYTEQNIHLVASLLKTFFSSLPEPFFPQELSPDLKTCLSIEDPTTRKNYMHGIVYKLPDGQYWTLRSLIFHLKRVVQHEQDNRMSLKNLSIVWGPTLISTPPTSEPYEQDSERDGRTDEITFQISAMETLFDVADQAFEPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZZ0</accession>
    <name>A0A1E5RZZ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">T-complex protein 1 subunit eta</fullName>
            <shortName evidence="10">TCP-1-eta</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">CCT-eta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g10</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin. In yeast may play a role in mitotic spindle formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterooligomeric complex that forms two stacked rings.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZZ0">
        <property type="interactions" value="1472"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZZ0"/>
    <dbReference type="InParanoid" id="A0A1E5RZZ0"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03340">
        <property type="entry name" value="TCP1_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000074">
        <property type="entry name" value="T-complex protein 1 subunit delta, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000022">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000006">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012720">
        <property type="entry name" value="Chap_CCT_eta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02345">
        <property type="entry name" value="chap_CCT_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ92293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="552" mass="60429" checksum="904C7FE9F3B7140E" modified="2017-01-18" version="1">MNFNNQTPTIVVLKEGTDQSQGKGQIISNINACMAIQETLKPTLGPLGSDILLVNAQGKTTISNDGATILKLLDVVHPAAKTLVDISRAQDSEVGDGTTSVTILAGEFMKEAKQFLEEGISSHVIMKAYRTACKVSMDTINEIAVDVTANNLGQRELLEXCAKTAMSSKLISANSDFFVKMVCDAVLSLDQEELDDKMIGVKKVSGGAMEDSLFIDGVAFSKTFSYAGFEQQPKFFEDPKILCLNVELELKAEKDNAEVRVEKVEDYQDIVDAEWTIIFNKLQQIVDTGANIVLSKLPIGDLATQFFADRNIFCAGRVSAQDMDRVIKAVGGSIQSTSTDIKPEQHLGTCKVFEEIQIGSERYNLFKGCPETKTCTLILRGGAEQVIAEVERSLHDAIMIVKRTIKNKIVVAGGGAIEMEISRKLRDFSKTIPGKQQLIINAYAKALEVIPRQLCENAGLDATDILNKLRMAHSLQKEDKNSKWIGIDFDTENIGNNFEKFIWEPAVIKINAISSATEAANLIFSIDETITNKQAQDQGGQVTGMRGRGMPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S075</accession>
    <name>A0A1E5S075_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transcriptional activator HAP5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g220</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NFYC/HAP5 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5S075">
        <property type="interactions" value="508"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S075"/>
    <dbReference type="InParanoid" id="A0A1E5S075"/>
    <dbReference type="OrthoDB" id="1272441at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22908">
        <property type="entry name" value="HFD_NFYC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000062">
        <property type="entry name" value="Nuclear transcription factor Y subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050568">
        <property type="entry name" value="Transcr_DNA_Rep_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252">
        <property type="entry name" value="HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252:SF8">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="9">
        <location>
            <begin position="232"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="186"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ92373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="321" mass="36571" checksum="1D225AB00F27B53B" modified="2017-01-18" version="1">MQDQHISLGLELRVNGFEHVQEHTNTGKPAERREPXEENEEHFDADMDNGVIVIDTYEPEKQELIEDEKXRQGREYIEHSKVDESPRVEPLAHDQMPEETQADTQPQIQTARVENECSGVENNDEDMSPSNYGAYYEYNDENEVIVKDEEDVFKNVALGLEGKYRDLLIDFWQTLINQIESLNENDLEDAPGNRETDRNPPPSSSFLSSSSSSSSSSSSSSSSSSSAVVGIQDSFKTHNLPLARIKKVMKIDENVKMISAETPILFAQTCEIFITELTLRAWTIADENKRRTLQKQDVVEALQKSDMYDFLIDIVPRQLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S091</accession>
    <name>A0A1E5S091_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cysteine desulfurase, mitochondrial</fullName>
            <ecNumber evidence="3">2.8.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">tRNA-splicing protein SPL1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g169</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters. Plays a role in both tRNA-processing and mitochondrial metabolism. Involved in the 2-thio-modification of both 5-carboxymethylaminomethyl-2-thiouridine in mitochondrial tRNAs and 5-methoxycarbonylmethyl-2-thiouridine (mcm5s2U) in cytoplasmic tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(sulfur carrier)-H + L-cysteine = (sulfur carrier)-SH + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:43892"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="2.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5S091">
        <property type="interactions" value="874"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S091"/>
    <dbReference type="InParanoid" id="A0A1E5S091"/>
    <dbReference type="OrthoDB" id="10250117at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990221">
        <property type="term" value="C:L-cysteine desulfurase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034227">
        <property type="term" value="P:tRNA thio-modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000003">
        <property type="entry name" value="Cysteine desulfurase IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000002">
        <property type="entry name" value="Cysteine desulfurase IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00331">
        <property type="entry name" value="Cys_desulf_IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010240">
        <property type="entry name" value="Cys_deSase_IscS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002806">
        <property type="entry name" value="PRK02948.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010611">
        <property type="entry name" value="PRK14012.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601:SF34">
        <property type="entry name" value="CYSTEINE DESULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601">
        <property type="entry name" value="CYSTEINE DESULFURYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="14">
        <location>
            <begin position="121"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ92425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="518" mass="57227" checksum="D3476576D176FA29" modified="2017-01-18" version="1">MLKNQMRLMTKSAVCARVVXAQHRGLASAAAKTAAKIVQQQEEHAHRLKDDRYKDFRLSVSTQLQNIGSKDASAIATEAINSGVTSLVEEAESKEASGIPGFSSVLKHAYQEQSDRGTRPIYLDVQATTPTDPRVVDVMMKFYTGLYGNPHSNTHSYGWETNNEIEKAREHVASLINANSKEIIFTSGATESNNMVIKGVARFYKKTKNHIITTRTEHKCVLEAARAMKNEGFDVTFLNVDDKGMVDLKDLEKAIRPETCLVSIMAVNNEIGVIQKLKEIGTLCKSKKVFFHTDCAQAYGKIPLDVNDMNIDLLSISSHKIYGPMGIGAVYVKRRPRVRLEPIISGGGQERGLRSGTLSPPLVAGFGEAARLMKEEFEADTQHIDRLSKRLTNGLLSIEHTSLNGHADQRYPGCVNVSFAYVEGESLLMALRDIALSSGSACTSASLEPSYVLHALGKDDALAHSSIRFGIGRFTTEEEVDYVIKAIKERVEFLRELSPLWEMVKEGIDLNSIEWSGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S0E4</accession>
    <name>A0A1E5S0E4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Flocculation protein FLO9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g77</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5S0E4">
        <property type="interactions" value="644"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S0E4"/>
    <dbReference type="InParanoid" id="A0A1E5S0E4"/>
    <dbReference type="OrthoDB" id="3973523at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000128">
        <property type="term" value="P:flocculation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001389">
        <property type="entry name" value="Flocculin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018871">
        <property type="entry name" value="GLEYA_adhesin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00624">
        <property type="entry name" value="Flocculin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10528">
        <property type="entry name" value="GLEYA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184970" evidence="2">
        <location>
            <begin position="26"/>
            <end position="1390"/>
        </location>
    </feature>
    <feature type="domain" description="PA14" evidence="3">
        <location>
            <begin position="91"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1195"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1204"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1228"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1245"/>
            <end position="1260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ92488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1390" mass="142656" checksum="8CBA1E6D596FCFFF" modified="2017-01-18" version="1">MFSNSIKTLLVFSVFFANFFNFTNAVGSTSQGDVVGCKPDNVALQGLNMTFYYYPWLKKSASGTSNTADLTTYKTDAYINGGYADYPILATASDVTNITYATNSKQIPNSATEVWGQLPSTMKATPNNVSLSNFAFLATGYIGIPDDYFGSDDIRESYSVTFTLNYIDDYGVVSIGDGIAYNCCASDTAVTQNGYQFQSLWTGGKPSGINTVTMTLYAGYYYPIRIFYVNRNYIGAITMTYSVEGETATDDMSRIMYMAADQEGGCSVPSLSTTISDYLYITAPTTVAGISTWTSTVSGVATTETGFAYVVEYPSLTTGTDVSYTSYYTGTTTSKFTTTTWYLNTDGVYELQTVVSEADPYSSLNTTTYKPWTGSVTTVYATSTQTVTNNGVPTVETVYQVLTPLQSLTTTTSYTPYDGPTTTTVGTTTTYLTTDGFTYTTVIVEVDTPAAFGTTTSFTAGSVASVTTVGTSMTTIAGSDGTDTVSTYYTVEVPIVSTTTTSFVEEVVEKTTTLATLVTTIVPTDGSPATVETVYKVAVPQKTAQFTVTNVADVPSTTTIATRYTTVQGSDGIDTTSTIYSVDIPGAFTTSVAVSTGDVLSRTTTRTSIITITGNDNMVTVSTIYAVEVPTPSATGTTTTYGDVASEVTIGTFFTTFVGGDGESTVSTLYTVEEPGTSVSSTSVVTGAVNATTTVGTAVATITGADGKATISTLYTVEVPTPSATGTTTTYGDVASEVTIGTFFTTFVGGDGESTVSTLYTVEDPGTSVSSTSVVTGAVNATTTVGTAVATITGADGKATISTLYTVEVPTPSATGTTTTYGDVASEVTIGTFFTTFVGGDGESTVSTLYTVEDPGTSVSSTSVVTGAVNATTTVGTAVATITGADGKATNTGRDTCEAPAQTSAGAAPSNGNVASEVTIASPSVSTYYTEYDGTVTSTYSTSVFTTTNAXGHXVVSTVYSVEVPGSPSVSTYYTTYGGSVTSTYGTNVFTTTDAEGHTVVSTVYSVEVPGSPSVSTYYTEYDGTVTSTYSTSVFTTTNADGQAVVSTVYSVEVPGSPSVSTYYTTYGGSVTSTYGTNVFTTTDAEGHTVVSTVYSVEVPASPSVSTYYTTYGGSVTSTYGTNVFTTTDAEGHTVVSTVYSVEIPTGGAIASTQTVTISGVVTDTTEYCPGLTRTTHVASSASEVSLSALSDKASLSDGGDAGFSTTSGKADSPTTSVKALGSTVPITSQGTPTSTGSGSAEGSNAFNNDRSSSDNGAFNNVASTIETSTVITATTLTPSTXNSSGANAVGSEIPAAAVSDVTXSGAKTSSAESSGAINASPAATGFATGSSNSGTSNGFGAPSVSAYAPSSSSSGVATAQIRTQYSAMGSKSHYSMITSIFAVFVFFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0A5</accession>
    <name>A0A1E5R0A5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 14">DNA replication licensing factor MCM6</fullName>
            <ecNumber evidence="3 14">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g4561</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the MCM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3 14"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0A5">
        <property type="interactions" value="1160"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0A5"/>
    <dbReference type="InParanoid" id="A0A1E5R0A5"/>
    <dbReference type="OrthoDB" id="1744952at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097373">
        <property type="term" value="C:MCM core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990518">
        <property type="term" value="F:single-stranded 3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902969">
        <property type="term" value="P:mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006267">
        <property type="term" value="P:pre-replicative complex assembly involved in nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17757">
        <property type="entry name" value="MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.870:FF:000002">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000003">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000115">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.870">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008049">
        <property type="entry name" value="MCM6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041024">
        <property type="entry name" value="Mcm6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF43">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18263">
        <property type="entry name" value="WHD_MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01662">
        <property type="entry name" value="MCMPROTEIN6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 14" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 14" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="16">
        <location>
            <begin position="559"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="96"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="251"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="256"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU368064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ80338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1032" mass="114400" checksum="8D9FAF1613AB03A7" modified="2017-01-18" version="1">MSSPPSQNPPSSFYDVNANNSQYLSSELSGSQQQQQQQHPSIQRGNGYGASRMANNTRIDSSRIDMSSEPNLLSSNNINDTSTFQSTMGIPEMSQSANSEITNGGANGGSNDQDDGDDMDMPLDGVSRRNMNAIRKVVDLTGEKVGEAFEKFLMEYEISVPNDSDDVSFQTNEELKKIYLKQIDFMFEIQINTLYVDYNHLISVENGTLALAVLEQYYRFLPFLVNALKRCIKIVKPQYLYTKDYTGVDTSSQEGGNAASTNSQNTNTAGTTGGTSVASTRGSETEGKNERIFQITFINLPATFRIRDIRADKIGSLMSIMGTVTRTSEVRPELFKASFTCELCQAIVDNVEQNFKYTEPSYCPNPSCENQSFWHLNVNRSIFIDWQRVRIQENANEIPSGSMPRSMDVILRGDCVEKAKPGDKCKFVGTEIVVPDVTQLGLPGIKPYSSRGNDSGMATSSEGLNSGVGGLKVLGVRDLTYKITFLASNVTSMGSGMTAQSSGDTSEESETIMKLLKDIQRLGAAEDEESTFSDREVIMAGLTKHEIKDLHSMINDGHVYSKLVRSIAPSVFGHEVIKKGILLQMLSGVHKTTVEGIKLRGDINICIVGDPSTSKSQFLKYVVGMAPRAIYTSGKASSAAGLTAAVVRDEESGGDFTIEAGALMLADNGICCIDEFDKMDIGDQVAIHEAMEQQTISIAKAGIHATLNARASILAAANPVGGRYNRKLSLRGNLNMTAPIMSRFDLFFVVLDDYNEKVDTELASHIIDLHMKKDEAIHPPYSKEQVLRYLQYAKTFNPIMNEEAKTYLIEKYKALRKDDSSTFGGRSSYRITVRQLESMIRLSEAIARANCTDIITKELVAEAYDLLRQSIIRVDMEDIEMEEDLPLTNEQATTQRVTTGVAENVPGNRPVTTITYDKYVEMLNLIVRKIAIESQTNNKELTANEIIDWYLSLNDYADTEQYMSERKICXKVLKRLVKDKILMAIRGTKENLDNSDDEDEAEQQSARDVQDVTKIVYVIHPNCAILDEMDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0P0</accession>
    <name>A0A1E5R0P0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">U3 small nucleolar RNA-associated protein 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g4579</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0P0">
        <property type="interactions" value="343"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0P0"/>
    <dbReference type="InParanoid" id="A0A1E5R0P0"/>
    <dbReference type="OrthoDB" id="4055624at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018843">
        <property type="entry name" value="Utp8_b-prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053881">
        <property type="entry name" value="Utp8_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10395">
        <property type="entry name" value="Utp8_b_propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22542">
        <property type="entry name" value="Utp8_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Utp8 beta-propeller" evidence="2">
        <location>
            <begin position="4"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="Utp8 C-terminal" evidence="3">
        <location>
            <begin position="436"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="711" mass="79717" checksum="A3729CF79B5FF0E0" modified="2017-01-18" version="1">MTCSIAQPFRVAPLPKISSLNDFSKQAAHLQLSQTSPIQQFSNNFNIGITGSSLSQYIINPTPKLIFNHPIPSTNVVSACDVMTIKPNKTXNSSDVSIQNSEAQNTADVSGNEQDSEKELWVYGLLANGRISSFNYFSRNPNNENESISVTTVKQEQKIANLKILSPKNVLIVFENGTFCVYNLQDKKEVSFTNISYGNLYFSHFFQSENSRKNFLMTLTEVSSDKPGIVFKLHEIFIKNDVVSVVELQSTVLESFTKIGNSNKMFYNSGKFYQLVASKIEVYSILPTFTRQHDSIDLSTLITTDADLEYASLKPTAPNRCLLSCQNKIYLLDLLHKALLFTKELSANVKSFQILQAGVLQFNQGSSTLALGVSTKHGATHSSFLDVVNINVGSGTLKEALGKSFLKNSANKTEGPQFKTLLKPLGSNGIPKQGVTPDLTLIYKDLVSQSSNAENFDKTFFTELRIDPAKGYYDESTDRFLNNAQFIEALCETTLTSFISKKQSPKVFTYLLTHPYFPHSKTSKLLSKLKFDPRLFKQCIVSCPALPIDELMEELFSVENDEILLELTLRILQDFNKEEIKNSIKIMSRLSVNNFITFVLNYKSIKNIQDNNIIETMPQIVQLLTLVLDSIGIFQLDLPLLKRLSKFLIKNIQIVETNDLLLNLLDNNSNFAANGSLGFSANTTFNSKKQNSKKLSSDKYVPNYSIETLQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0P9</accession>
    <name>A0A1E5R0P9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Flocculation protein FLO1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g4390</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0P9"/>
    <dbReference type="InParanoid" id="A0A1E5R0P9"/>
    <dbReference type="OrthoDB" id="4070743at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000128">
        <property type="term" value="P:flocculation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001389">
        <property type="entry name" value="Flocculin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018871">
        <property type="entry name" value="GLEYA_adhesin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00624">
        <property type="entry name" value="Flocculin"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10528">
        <property type="entry name" value="GLEYA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56988">
        <property type="entry name" value="Anthrax protective antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184553" evidence="2">
        <location>
            <begin position="31"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="domain" description="PA14" evidence="3">
        <location>
            <begin position="70"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1101"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1102"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1118"/>
            <end position="1129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1316" mass="141346" checksum="F479AD677CB8F961" modified="2017-01-18" version="1">MLTQKFGTTYFKTASLLLIFIFLCVGKTASAVSASSSSGYLSACRFPTTYSLNSGFDLKFYYYPQRSSQYTDPGFYVSEYSTYSYLGQGKNVETLTYTNNYVQQNVYSTSLPGYFRYSGAKFDSDHFTMVGTGYFKATVSGIYTFQLNWADDYSLISFGADNAFPCCQQQNGHLSTPYSDYTVSNDLAYGDKKTLQVNLLAGRYYPIRVMYINISHLATFSFQFLVPGETTYRTDFTGWVYSGETDLSYVCTDNIVTSFVEWSSTYLSTLETETYATFTTDNSIVVSDTVEEQIIYTPKPTTINTFTTLTTTVITVITTEIAVTTVTNTANSQVSVITRQTVEIPLSYTTTTSYSPYRGATTSTYSSTTVVSTASNSDVAITTIYYVATPIVYRSSTLLTPSPKLNSTTTYSILVSTSTGTDGKETDVTVFYVGTPIVSDSSTIHTPYSGSINVTYLTEYTTTTGTDGKETDTTIYYVSTPIVFTTTTLIQPSHVLSTLTYSTSTITTTGVDGKETISTLYYVETPIMEVLSTVYTHYSGLVNLTYATSFSNSTDTEGRETDTTIYYVKTPIIHTSTTVLTPAAVPSTTTYSSTVVTSTGIDGKETEATVYYVETPIITVYSTRCTPYSGSVNVTYTTSYFNTTGTDGKEIDSTIYYVSTPFTNSSQSAVSSSSFVPSQRVTIFSTIEKFYNGSYKNTYSTSRTTLIGPNNFETDSTVYYVNIPIIHKSSTSFSPANIQSTRTYSTAILVSSGPDGVENDFTIYYVETPIVTIYSTIFVTYSGLTNTTYLTSYAKTTGSDGKETDSTIYFVNVPEPTLSSCTATSCFNQATNSTASSSTTKSSSLNTLTKSSSLFNTSSFSSSSSFSASSSTSSSSSFLSSSAFSNPNTSFSSTSLSISSNQFVPSNASTSMISSLVTSTSSSSQSKTDSSSSFSSQSANFTATALSSSSSIISQMSFSSTFTSSFSKMGSSSEPVSSSKSAFDHTSFSSSFLSSTTNSSMMSQSSVLSTPSTSLTPITNSSKFTTLPETNLPVPASLLNTSAASSTSTFTSVVTLSNSIATLSSSSFSFSLTNYVPSFFYNTSSLHSTFSSSQSSLNFKSMTPTHSSPSTIETSTRALSNTDSDTSASSSLLTSSFVTKSVASSDNTFSSLSSTEEFSESRIATKSSSPTFELFSSSTSQVSQSTESLNSASIPISGSSNFNTAVSKSFTALSPSNSGTERGLPFSLAPEPTTTTISQQLFSTSLSSAPSLPSLPSVATFVSQRQTSNSPSLSSVIASSTELIPTQYQAVGSTRHPKKGVLFLFFSFLTHIILFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0R0</accession>
    <name>A0A1E5R0R0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein RCR2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4498</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R0R0"/>
    <dbReference type="OrthoDB" id="3972519at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020999">
        <property type="entry name" value="Chitin_synth_reg_RCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28187">
        <property type="entry name" value="PROTEIN RCR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28187:SF1">
        <property type="entry name" value="PROTEIN RCR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12273">
        <property type="entry name" value="RCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="286"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="335" mass="36949" checksum="13FAECD61A56B4BA" modified="2017-01-18" version="1">MCASVATPTIPTTTLYKRQDQEKADATSSTSSSSSPSSSLYPSSTSTSSSDDMGWFQNNSWAWQRWSIFAVFILVILFAVVATVRANAARARRGRKPITGTSWFTPPSYLQSERQYNHDDGTRVDRRNRDNHGANGDEYVPQYTEEVNPDDMGYYDDKGLFHVNSKALPLPPPPXLEQGLSGIDTHGNSRGNIGSGSIRDGYDYYNSSQTNGNHGGDANHFNANEQLDNLSLASPENAMIRQRAREQYYSLGNVPVVHSSSAVGNSVSXHTNVSSPARESIEMTRINTASNDSNDNIGDSELVNNNDNDNDNGRLRLSVQEQVVRARNLQEEKHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R100</accession>
    <name>A0A1E5R100_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4554</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R100"/>
    <dbReference type="InParanoid" id="A0A1E5R100"/>
    <dbReference type="OrthoDB" id="3973113at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="2">
        <location>
            <begin position="718"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="391"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="650"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="321"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="368"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="391"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="672"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="783" mass="85689" checksum="DD2AEF6DDB775C32" modified="2017-01-18" version="1">MNFSSSIFDQVDHFMHDGSTNNTTNNTTNNTTNNINNNTTNNIDIDNTSSNKDSNTNNSDTKQYLEVRSSIASTPVTQEIVQGNNQQTFFGNSPNPFLLANQTPNFNKNTGNTPFDHSVNLNFEIEQNQQKTSVVELEAHFDELQKHFENSFAPNSELNFASPNTVPSTANITTDSPHNIXNNSHNNINXNSHNSSDNAFTDMATAFKDSSRDKHAHLLEMEQMGMMEGGSPSISPTVEPKSLSNMNTPFLHANSAXLNEKNAKPRNTSLSINTLPSPALKSIKATPDMTDTKMADNNNHNGITTNSASNSIDNIPGSGGSSRLDSPSSVSSSGYKRPSKSKTSSALNNSRKXTKNSPLQKPKKSPMIRSNASASMSKKFSNYSIKSNXVMSFNNNNNNNNNNNNGSANSNRSSSIDHAAGPGDWDEMFALPASSLPNEHLHANNHMNGYTKDPSMSPFTSGVSREERNFSKTNNTAGIKIILPSSASEGSNSVEADRATKDVAXTEGEFSQDKKLQMKKFGFSGSPLLNMNNTYSADRXSRQKEHGVETTXLRATESPVLRARKSASLTPLMGAKRASSLEGSNLNQQQGSSVHYSKDYCNKQSSQYMNLTSNPDPMQMMSNDLRETDTFGYQSRTSSVEKLPAASAFHELKKTTSNGNPTGKQVXRKSSESSNSSNININNNNNNNNNDNSLGHTAKDVSYAHINDHDSKELFPHGKKEVHKEAEKGRRERLNNALAELGTLIPIELRATMKVPSKATTVELACEHIRQLTEKVKKYEGTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R159</accession>
    <name>A0A1E5R159_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">MAP kinase kinase kinase SSK2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g4270</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R159">
        <property type="interactions" value="417"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R159"/>
    <dbReference type="InParanoid" id="A0A1E5R159"/>
    <dbReference type="OrthoDB" id="1043025at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038066">
        <property type="term" value="P:p38MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06626">
        <property type="entry name" value="STKc_MEKK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF32">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="1640"/>
            <end position="1911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="214"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="504"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1794"/>
            <end position="1814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="76"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="221"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="236"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="254"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="517"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1794"/>
            <end position="1811"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="1669"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1939" mass="219555" checksum="D1ECD7D006F76F52" modified="2017-01-18" version="1">MSSTTESNASHDKNXEDHSEYGTPTDYITTTTTTPSHLNDVSYSGVDTEEGKILNSHILPESHMSVSKPSRKSSRSRSNSVMSSSRLFQQKEYPSSTSMKISQSLQSINTAIRDXANDYRRSSIDSTASSPSSANSVKFVTASINSLLEKNKSNDQFTLGNKEDAENNFNGQSDEEEINLNISKPLLSNIITSHLSLPDLSAANRYKDRRNVLFSPPATDLKNNPLLQANPMNAMSNEKSGSSNNNSRSGSINAPEQINLPNSSKPSVLKHTNGSPKYNITSTANVSTANVSTANTPTAATTTIPLERSKTTTCISTESPLERARTNSNTSGISLVSNTLVQPSPTSSIRPVSVGTNHSFIKPMYLQQQYAINEKQYLKKIKNTKSSQNDYYNRGIFDNSNSSFNIHSFYQKNENANETDLQPSLNGYTEIPTTITNLNTCPTPPITRNENLKMVPLSPKSDHPTESSFKNDYFFNQNVEKNTALDSSPVIQKSIAAQRTSALSTKAGNGASPVVKTKNNISPNESSFAQKPLNLKAKSKQSTKHAHIPTKTTLPLSEDVLKNKMLNKIKDKNVCIIESDYIKELKKYSTFSENDEDFKNGLSFDNNSADIDVTPYRNTELDDYISDQAYPIDGFSQFSGDSNTSTVLPDNLHENDNEIPPTEHKRTKSLSNNLLAILPRSSSPALSARSQSNYAFPIELAADEDDFQMQERLEWQLMLTRVLKGDIVQNEKSKLSELKEQIGGHETDFKNNIWIELKAWMNGHTVPDQIKTLDMLKNSVADDVFQKILNFQFNTEVPCKEGFQQTFQLLDEYYKVVAHWKTLKDMHEEKPVTATKEFQDVVNALTCWRNLSNAFTNLILDILPFTNLNEYLGNEYSLLFAGKTYKDVVNYTLDPEYVDKFIKEEDIEAMFQKKIFFKQVPWIFKAQMVKFRYEEVYSARHIPVDLDRLKLVLFYPFKLIKEIIKKRLEYSKSLVDPTMMIIDQLIDDLCVQIKLATQIKFSVMQSPFFRLDWDDHTSYDEVLQTAVQHVFEMTNLKLASKSTSIFKSSKDIDELLQQWDNFKNLGIFINEKIGLDIASNYAKLFLQMSMKLHIYVLKKCTRVPDFKKEQDLEKWVFESMENVGSVKRKFERFMGKMCGSLVNQANYTFQDENWNTVLENLVASNHTLLYTGGELESYGIYALISKEIVEKGSYKKDDILKILNNSSICSDVVPTINIKNSLHVYGNENIYQIYSEDPESKVYWKTHTENGISYHNLVVGDNNPSTKSFEASNEPKEGVASQKKEETLENIYKGEQGYTDEENEEYEKKRLRQMEETLEQNGYLILLKADSSMLWEGSTIILEEPVLSSEIKLDLQKLLASDNFNLSLLTEGSPYSTDYQMRKFHSRMNEALSLVNTVCSVAPIENMLQKINRVFYKMTTSVLNEYPKTVTKLDQKFPGSEILNNGFMFTRDFARSYLVAHEHQLNQERKAEMVMSIMQLAIEWVGFICDKCNPTDLRTFRWCVPAMEYAMKITSGHNILALSAEQYSDLKRKISGCMSLLISHFDVMGGRARAMAENKYLIAAQQKSAGSQVMLDDYDDDAVLEVNSQMRLQSINELEKQYRKRTKQKKKIGKVLNDFDREDKYLAALASSISNVSIKWRKRQFIGGGSFGDVYSAINLDTGGILAVKQIKIQHSKNMEKIYPRIKDEMNVLEVLNHPNVVQYYGVEVHRDKVNIFMEFCEGGSLASLLEHGRFEDEVITQVYSLQLLEGLAYLHHSGVVHRDIKPENILLDHNGVVKYVDFGAAELISKNGTRKYSSTRSRNGKQNSMMGTPMYMAPENITGRRKGIQLGADDIWSLGCVILEMVTGRRPWANLDNEWAIMYHVAAGHIPQFPTSDEMSGVGTKFLSRLLKHNPDQRATAVELLLDPWIAEIRKIAFSTDDTSGSSSSSSSVLTTPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R174</accession>
    <name>A0A1E5R174_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein SGM1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3848</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R174">
        <property type="interactions" value="520"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R174"/>
    <dbReference type="InParanoid" id="A0A1E5R174"/>
    <dbReference type="OrthoDB" id="74178at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052602">
        <property type="entry name" value="Growth_transcription_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022091">
        <property type="entry name" value="TMF_TATA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46515:SF1">
        <property type="entry name" value="TATA ELEMENT MODULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46515">
        <property type="entry name" value="TATA ELEMENT MODULATORY FACTOR TMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12325">
        <property type="entry name" value="TMF_TATA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TATA element modulatory factor 1 TATA binding" evidence="3">
        <location>
            <begin position="621"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="209"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="273"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="631"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="740" mass="84397" checksum="797FBF5E900C62C5" modified="2017-01-18" version="1">MSEPSQKLTLEQRLELATKSRKKGRGKNKSQAKDETSKEPTPNVDGSTAEDVRKEXSATPIASNDTPSIPPMXXQSLDLANDTKEELTEATQIAVNRDILKETKPEPVELQEGPVDLETLEVGGKAASSIDSQSTSNIAFSPEVLKHLSLTNEDLKLPLNDFLARLETQTLNYVRSVNSNILKKDETINQLRKEGNALSKKELKLSDTVKALKKDKTKLEMKLEICQEELSSKINESEKVHSEFYTIKEKHDALQDSLSSTVKERDLLSKKLADTTDIEVSGLKSELEKLKSENEELKKKYNSVIAEYDQYKSKSLVQNETLKTSSDEEIAILETKLEQLRIKLEDQTARENISHQDAEKSEYLDKLQAKYNQLTRELQKTNENWSNIEQKLHNRLLDLKNDYEKSTTLQKELTVKIQKSDQKKEKLLQELNKERATTAAAEKKTSELQSQLDLLVHQLESAKRDAQMLNDKIKLQNKQLEEMFTPSFRSADQKKEKTPRSSVAPLYRLSVHKEEEENESDTEGYDAKNALKLQLENEWDIKPAVKDKNVDLQSEAGSLHDPHSASASRLDLDVPTPTGFQSVRSFSRNNSYVSQQFAEDNEDPILGRRVSNVVSTPTQQYSSNPTSTQLMARMGGQVRRLETELASLRDSWDKLQKEKSQAQEEILRLMMENENVISIQKQNTELSQSLQSLKKEQQQSKMQLDSKQRKIHELENDVADLKELMHSQIQQMVELQEKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R196</accession>
    <name>A0A1E5R196_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CTP synthase</fullName>
            <ecNumber evidence="9">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g4197</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>UTP + L-glutamine + ATP + H2O = CTP + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the CTP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="9"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R196"/>
    <dbReference type="FunCoup" id="A0A1E5R196">
        <property type="interactions" value="948"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R196"/>
    <dbReference type="InParanoid" id="A0A1E5R196"/>
    <dbReference type="OrthoDB" id="1739076at2759"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097268">
        <property type="term" value="C:cytoophidium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000207">
        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000002">
        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003792">
        <property type="entry name" value="PRK05380.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00337">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="CTP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550:SF0">
        <property type="entry name" value="CTP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTP synthase N-terminal" evidence="11">
        <location>
            <begin position="2"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase" evidence="10">
        <location>
            <begin position="316"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU810713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ80671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="578" mass="64818" checksum="90B6EA1F02A5E3F1" modified="2017-01-18" version="1">MKYVVVSGGVISGIGKGVLASSTGMLLKTMGLKVTSIKIDPYMNLDAGTMSPLEHGECFVLNDGGETDLDLGNYERYLNIELTRDHNITTGKMYQYVISKERKGDYLGKTVQVVPHLTNAIQDWIERISKIPVDNTGLEPDVCIIELGGTVGDIESAPFVEALRQFQFRVGKENFCLIHVSLVPVIHGEQKTKPTQAAIKDLRSLGLTPDMIAVRCSETLELPTIEKIAMFCHVEPEQVINVYDVNSTYHVPILLHEQNLLATLDKKLKLHEQCEILPNLKVKGDHLFQNWSHLCSNFDSSVDTVEIALVGKYTHLKDSYLSVIKALEHSSMKLSKKLQINWIESSDLEDPVNNKKQWEILKKSHGVLVPGGFGSRGIEGMILAANYARVNKIPYLGVCLGLQVATIEFARNVLNIENGSSCEFDDTLDPINDEKAIVVYMPEIDQKNLGGTMRLGLRPTIFIDGLSDHSKIKKIYGDYSSVSERHRHRYEINPKLCSRLEEKGLKFVGKDESFQRCEILELDNHPYYVGTQYHPEYKSKVLDPSPPFYGLLAAAAGVMDEKFLQDCVNNKQNEEVMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1H7</accession>
    <name>A0A1E5R1H7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4339</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1H7"/>
    <dbReference type="FunCoup" id="A0A1E5R1H7">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R1H7"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035303">
        <property type="entry name" value="DUF5364"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17322">
        <property type="entry name" value="DUF5364"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="251" mass="28748" checksum="46B8160883AB0760" modified="2017-01-18" version="1">MDYALKAENRKKFQDKQKLKRKHLTQSDRKYIKEKYRATAQSEENPGEKNDDNKESVQDSGSEDNESLRDELKLEQIDFSGEKTQGGKLFEKRKLQSNTYRYDKEQDEEFHNRVNKIVDDSLINDELRAQLRDLALKKKTEESNSRDEEQGGDGKVLTSANVKRKDLDSLTTKQLNELLTFGKARIGQDDGNGEKNEITRNSKLKTLEHSPRNVSNESNKRLNVTTPLPSAPTSSVPSGLQEEQDFLDGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1Z8</accession>
    <name>A0A1E5R1Z8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">sterol 14alpha-demethylase</fullName>
            <ecNumber evidence="17">1.14.14.154</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Cytochrome P450 51</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Cytochrome P450-14DM</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Cytochrome P450-LIA1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Sterol 14-alpha demethylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="33" type="ORF">AWRI3579_g4142</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="34">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>32-hydroxylanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 32-oxolanosterol + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:75107"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:166681"/>
            <dbReference type="ChEBI" id="CHEBI:166806"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:75108"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>32-oxolanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:75111"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:17813"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:166681"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:75112"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>a 14alpha-formyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a Delta(14) steroid + formate + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:68068"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:138031"/>
            <dbReference type="ChEBI" id="CHEBI:176902"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:68069"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>a 14alpha-hydroxymethyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a 14alpha-formyl steroid + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68064"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:176901"/>
            <dbReference type="ChEBI" id="CHEBI:176902"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:68065"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>a 14alpha-methyl steroid + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = a Delta(14) steroid + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54028"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:138029"/>
            <dbReference type="ChEBI" id="CHEBI:138031"/>
            <dbReference type="EC" id="1.14.14.154"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:54029"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>a 14alpha-methyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a 14alpha-hydroxymethyl steroid + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:138029"/>
            <dbReference type="ChEBI" id="CHEBI:176901"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:68061"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>lanosterol + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:25286"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:16521"/>
            <dbReference type="ChEBI" id="CHEBI:17813"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.154"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:25287"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>lanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 32-hydroxylanosterol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:75103"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16521"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:166806"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:75104"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 30">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="16">Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 31">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="33">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.154" evidence="17"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1Z8"/>
    <dbReference type="FunCoup" id="A0A1E5R1Z8">
        <property type="interactions" value="573"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1Z8"/>
    <dbReference type="InParanoid" id="A0A1E5R1Z8"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008398">
        <property type="term" value="F:sterol 14-demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11042">
        <property type="entry name" value="CYP51-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000033">
        <property type="entry name" value="14-alpha sterol demethylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050529">
        <property type="entry name" value="CYP450_sterol_14alpha_dmase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304:SF2">
        <property type="entry name" value="24-HYDROXYCHOLESTEROL 7-ALPHA-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 30 31" id="KW-0349">Heme</keyword>
    <keyword evidence="9 30 31" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 32" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 30 31" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="33" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11 31" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 31" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="15" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="10" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="14" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="33" id="KW-0808">Transferase</keyword>
    <keyword evidence="32" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="32" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="55"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="30">
        <location>
            <position position="467"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00037887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00038974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00043106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00043156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00047702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00048479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00048866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00049163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00049450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="EMBL" id="OEJ80926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="530" mass="60937" checksum="82EFF779BC933BF5" modified="2017-01-18" version="1">MGSFVEQGFEQLKFLFENFKALSTGQQISILVLAPFILNIVSQLLYSLRADRPPMVFYWIPWVGSAVSYGMAPYDFFKQNQEKYGDVFSFVLLGKVMTVCLGPKGHEFVLNAKLADVSAEEAYTHLTTPVFGKGVIYDCPNHRLMDQKKFVKGALTIDAFRRYVPLIAEEVYQYFGQAKNFKMNDKPKGIANIMETQSELTIFTASRSLLGKEMRAKLNTDFAYLYSDLDKGFTPINFVFSHLPLPNYRKRDEAQQKISSTYMELINERRRNNDIQDRDLIDTLMKDSTYKDGVKMTDQEIANLLIGVLMGGQHTSAATSAWILLHLAERPELQQELYEEQMRVLKNGELELSYDLIQEMHLLNQTIKETLRLHTPLHSIFRKVMKDMHYPNTSFVIPKGYHVLISPGYAQMQDKFFPNAMKYDPHRWDADFQSSYGTNEKVDYGFGAVSKGVSSPYLPFGGGRHRCIGEHFAYCQLGTIMSIFIRTLKWDFPKDIMKDGVPKPDFQSMVTLPTAPAFIRWEKRNPEQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R211</accession>
    <name>A0A1E5R211_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Coatomer subunit delta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4381</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9 10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9 10">Cytoplasmic vesicle</location>
            <location evidence="9 10">COPI-coated vesicle membrane</location>
            <topology evidence="9 10">Peripheral membrane protein</topology>
            <orientation evidence="9 10">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9 10">Golgi apparatus membrane</location>
            <topology evidence="9 10">Peripheral membrane protein</topology>
            <orientation evidence="9 10">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the adaptor complexes medium subunit family. Delta-COP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R211"/>
    <dbReference type="FunCoup" id="A0A1E5R211">
        <property type="interactions" value="1171"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R211"/>
    <dbReference type="InParanoid" id="A0A1E5R211"/>
    <dbReference type="OrthoDB" id="10266042at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051645">
        <property type="term" value="P:Golgi localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09254">
        <property type="entry name" value="AP_delta-COPI_MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14830">
        <property type="entry name" value="Delta_COP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000020">
        <property type="entry name" value="Coatomer subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027059">
        <property type="entry name" value="Coatomer_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10121">
        <property type="entry name" value="COATOMER SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10121:SF0">
        <property type="entry name" value="COATOMER SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4 9" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="12">
        <location>
            <begin position="279"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="167"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="190"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="202"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ80945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="533" mass="59956" checksum="495AF0FE5DB04FD4" modified="2017-01-18" version="1">MVVLAASITTRAGKPLLSRQFKDITKDRVTELLSNFQNLVANSNSQHTFVEDEHVRYVYKPFEDFFIILITNRQSNIIQDLDTLNLFSQTVNATLRGFDENDIFHNAFEILGSFDEIISMGYKENLSLSQIQTFLSMESHEEMIQEIIDRGKEFDAKEQRKRAAKEIMRKEHDRRMGGGQYSEYLDQPKFGTNNNQTNMNDAYNSYYSNASNAAQQSYQHLQKQQQQQQQQQHPHQHSPVAEVASTHTSQKRGRISKIGSTPASNIIITQGLIDEPENTSGVLITTKETISAQITREGDITSSELKGVLELRVNDSQYGKISIQLDSNKVDFKSKALNFKTHPNIDKKQFLQDGVIGLKDSNKSFPPNDQSLGVLRWRKTGGSDDDSIIPLKVSTWVSESSNGLTVTFEYELNEDSYNGGQLQDIYFIIPILNATASVTDGDAIIKGYDDEQGIVLYVKDLQQGEPGVFNVEIENVEDEDSLFPINVTFINPNGSSDISLCGVTADQIVNTEDGQPIEYQALSTLKNDEYVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R247</accession>
    <name>A0A1E5R247_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4290</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R247">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R247"/>
    <dbReference type="OrthoDB" id="4083656at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="126"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="329" mass="37179" checksum="21BC2690562EFE97" modified="2017-01-18" version="1">MFKSRLSRTLNFKRNAHSNSVELSALLKHMVTQKAPEVIYKYQPSKLVKFGSWSLALTFITYGATFGDWAFLTTQSNLEEPANSKTLTSNDEFVSENNNVSQQKHPLAVKIENLYDQKINTPQNRYYFANAGIAVLTAIPFVLASAALYFPGRIVTTAKFLPLSQQMQLTTLYNRTTTINISKCEIKGKLYTGVGPQLCDDKASFSVFLLNNADISKKRLSFNWVRNLFILSRSGQFFQNDCRFFDVLFMKKEILEEAAKLNRFKAHAPATTAKDQSSGKDGSLGLKNXSIKEDEVLKGAVGQVKKHVVPKPIKPTKHNHKRKHSTKRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2B8</accession>
    <name>A0A1E5R2B8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ATP-dependent rRNA helicase RRP3</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">ATP-dependent rRNA helicase rrp3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">AWRI3579_g4016</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent rRNA helicase required for pre-ribosomal RNA processing. Involved in the maturation of the 35S-pre-rRNA and to its cleavage to mature 18S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with the SSU processome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DEAD box helicase family. DDX47/RRP3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2B8">
        <property type="interactions" value="1159"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2B8"/>
    <dbReference type="InParanoid" id="A0A1E5R2B8"/>
    <dbReference type="OrthoDB" id="10261904at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17954">
        <property type="entry name" value="DEADc_DDX47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000626">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044765">
        <property type="entry name" value="DDX47/Rrp3_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF24">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 18 23" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="22">
        <location>
            <begin position="88"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="20">
        <location>
            <begin position="119"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="21">
        <location>
            <begin position="318"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="479"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="17">
        <location>
            <begin position="88"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="73"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="479"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="498"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="OEJ81041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="507" mass="56548" checksum="3EED8F5FFA45AFEE" modified="2017-01-18" version="1">MANTKDKVVKKASKSSGNGKSDSSLMSLAEKIKLRALENAKKQQQDRVSEEEKEQGEENGTALQGSHGQADQGDGEEEEEEEEEASFTSFSELDLVPELIEACKELNYTKPTPIQCKSIPPALAGSDIIGLAQTGSGKTAAFAIPILNRLWYDKQPYYACIMSPTRELAQQIKETFDSLGSNMGLRSVCIVGGMNMMDQARELMRKPHVIIATPGRLMDHLENTKGFSLRNLKFLVMDEADRLLDMEFGPVLDRILKILPVQGRTTYLFSATMTSKIDKLQRASLTNPVKCAVSNKYQTVDTLVQTLMVVPGGLKNTYLVYLLNEYLGKTMIIFTRTKANCERLSTLCNMLDFSATALHGDLNQNQRTGALDLFKAGRRAILVATDVAARGLDIPSVDIVINYDIPVDSKSYIHRVGRTARAGRSGKSISLVSQYDLELILRIEEVLGKKLPKESVDKQMILQLRDTVDKANGEVIMELNRRSKEKSNRRXSNRKKFGKDDQDREER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2H3</accession>
    <name>A0A1E5R2H3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Very long-chain fatty acid transport protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Very-long-chain acyl-CoA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">AWRI3579_g4211</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Acyl-CoA synthetase required for both the import of long chain fatty acids (LCFAs) (C14-C18) and the activation very long chain fatty acids (VLCFAs) (C20-C26) by esterification of the fatty acids into metabolically active CoA-thioesters for subsequent degradation or incorporation into phospholipids. The transport and fatty acyl-CoA synthetase activities are genetically separable and are thus independent activities. Esterifies VLCFAs in the peroxisome matrix. The VLCFAs are actively transported into peroxisomes by a PXA1-PXA2 heterodimeric transporter in the peroxisomal membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a very long-chain fatty acid + ATP + CoA = a very long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:54536"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58950"/>
            <dbReference type="ChEBI" id="CHEBI:138261"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2H3">
        <property type="interactions" value="136"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2H3"/>
    <dbReference type="InParanoid" id="A0A1E5R2H3"/>
    <dbReference type="OrthoDB" id="10253869at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044539">
        <property type="term" value="P:long-chain fatty acid import into cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000019">
        <property type="entry name" value="Long-chain fatty acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107:SF15">
        <property type="entry name" value="FATTY ACID TRANSPORT PROTEIN 3, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107">
        <property type="entry name" value="LONG-CHAIN FATTY ACID TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="13" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="21">
        <location>
            <begin position="108"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00060276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00068795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="OEJ81105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="677" mass="78045" checksum="21043BA635E96459" modified="2017-01-18" version="1">MSDEPKLVILSAIWNVLKLVFGIIAVIFKSIYNIPFIQKILKKHRLIEDFHIAPYFFTALFKYFISVKTKKFQYWYIFEEKVSTQPTQLCISYPKPTGAGKNSGKPKFQVEQYTYKETYEIVLRLSYYFVHELHFQPGDTIGVYYTNKPVFIFMWLALWNIGCVPAFLNYNSTGTPLKHSLDIANIKDVLIDEQCSDTFQIDSMDSYILHHVDELKMYKNVLLNTEYQQFRQLNSVRSPTTGKDYNPACLIYTSGTTGLPKSAIMSWRKACIGCSLFGRIYHMEEKNSVIFTAMPLYHSTAALLGVCAVISQGGCVAISNKFSASSFWEQVCLTKATHIQYVGEICRYLLNTMPNDFEKQHQVKIAYGNGLRPDIWVEFKKRFQIPIIGEFYASTEAPFATTSYQEGYDFGVGSCRNYGPLVTWFLNFQQVLIKVDPEDSSQVYRNSKGFCEACKPNENGELLMKIFFPKKPETSFQGYLGNKKATESKVLRNVFKKGDAYYRSGDLLKSDENQLWYFVDRMGDTFRWKSENVSATEVENCLMDSQPADKQYIDECLVCGIKVLNLEGRCGFAVIKFKEPLSKKIPLEMSSPEIFLNALLPSLQKNLPKYALPLFVKFADEIEHTHNHKVKKTAYKDQXLPHGENTKETIYFLKNNKKYVELTDQEWEDILSNKTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2H9</accession>
    <name>A0A1E5R2H9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription activator GCR1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3914</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R2H9"/>
    <dbReference type="OrthoDB" id="4061572at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022210">
        <property type="entry name" value="TF_GCR1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12550">
        <property type="entry name" value="GCR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription activator GCR1-like" evidence="2">
        <location>
            <begin position="329"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="159"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ81102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="426" mass="46869" checksum="86A6BF1ECED9F72C" modified="2017-01-18" version="1">MSNIIDTGNIIQDIVDKIDEFLVEELNEADKAKSNGLLTVDVNALKGLLGTIKDELKPMQDMNSQEIKSLQEENKELQQRVMALETLLTNNQQQQQQQQQHQTPFTNDGSGXNNSNTAAVARAAAAAIAVSSLRSAGRETITSGNISNTNDTXDDDDINNNTNNNNNNNNNNNNNNNNNNXNNKNNNDNNSSNNNNSNHNNNSNTSSHTNITGKKRSRNGKAIDDKLDVSGKSEADXNGSTNTTTSEDADGLSETLQAVSNTSSALPNIASAQLNVANNGTPGVDGDNSLKQSNYVPVSNFKPENLTKRRKKWAQDKIADIETFQLMITEAPESVAKLYNEFETSVKFQIQQFELQYGKGKLSKIPKIRTYQRRAALVREIQSYASANNCSVDESIAFFDKLVTDNKKTVAWLYNNLAKVLNDNMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2P0</accession>
    <name>A0A1E5R2P0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Decapping nuclease</fullName>
            <ecNumber evidence="7">3.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g4374</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Decapping enzyme for NAD-capped RNAs: specifically hydrolyzes the nicotinamide adenine dinucleotide (NAD) cap from a subset of RNAs by removing the entire NAD moiety from the 5'-end of an NAD-capped RNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside-ribonucleotide in mRNA + H2O = a (N(7)-methyl 5'-triphosphoguanosine)-nucleoside + a 5'-end phospho-ribonucleoside in mRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66928"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:17313"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:172876"/>
            <dbReference type="ChEBI" id="CHEBI:172877"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:66929"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 5'-end NAD(+)-phospho-ribonucleoside in mRNA + H2O = a 5'-end phospho-ribonucleoside in mRNA + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60880"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:15698"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:144029"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:60881"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 5'-end triphospho-ribonucleoside in mRNA + H2O = a 5'-end phospho-ribonucleoside in mRNA + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78683"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:17164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:167618"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:78684"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the DXO/Dom3Z family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.-" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2P0">
        <property type="interactions" value="636"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2P0"/>
    <dbReference type="InParanoid" id="A0A1E5R2P0"/>
    <dbReference type="OrthoDB" id="5853397at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034353">
        <property type="term" value="F:mRNA 5'-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110155">
        <property type="term" value="P:NAD-cap decapping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013961">
        <property type="entry name" value="RAI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039039">
        <property type="entry name" value="RAI1-like_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12395:SF9">
        <property type="entry name" value="DECAPPING AND EXORIBONUCLEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12395">
        <property type="entry name" value="DOM-3 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08652">
        <property type="entry name" value="RAI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RAI1-like" evidence="9">
        <location>
            <begin position="18"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="166"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ81162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="429" mass="49689" checksum="A9FE62A4CED738F3" modified="2017-01-18" version="1">MMIKPLFIRERSNVTALKQPKEIGRYSRKSYKENVENESXKQNCSTAGYKSKYYINDDIFLNYYYLPNDDLDKRIINLSGGFNKFKNASPVDDLSLHGLLATLIDIESKKNKKSKVDVVTMRGIIKSLIMLFNSLGSRNTTASSTDVDLQVVTFDGQIFIKKAGSLNSQHQETQEKPSTNESLTRPVGSRFSNTTRESQYLKKCEYSGYKFETCATLPRPVSEIPREKLDKRYKKIGTNDEQYNILVKTGIGNCKMILGAEVDAVFDFKEESQKIGEVPENLKHYLELKTSKMITNAHQAKTFENKLFNAWLQCFLVGVPRITYGFRDDDFNLVAVEEFLTNEIPVNLKNMNSLVSNHFGDSIKWYGAVVEWLLKNVEYKEGQENYYRLFYQDNYLKLEKIPDXDTEKVXEYKNXISDEFRKWRLSLQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4H4</accession>
    <name>A0A1E5R4H4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Heat shock protein SSQ1, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3615</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4H4"/>
    <dbReference type="InParanoid" id="A0A1E5R4H4"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000003">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="672"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="204"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ81817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="746" mass="81439" checksum="F454162D83915FFF" modified="2017-01-18" version="1">MLRIPLKANHQRIIHNTCSIFTRYSSQARSSPRNTKVLGIDLGTTNSAIAYHNPHTHTTDIIENEFGKRTTPSVVSYIPSTSTSGPLKEPWKVLVGDPAKRQQVLNPKSTFHSTKRLIGKSYKEVVESGELGVHAKHDQMQYEMAPLASGECGVVLNLPKGENPGTGFGTSQVIKSPADVGSXVLKYLLTQARHHFFGQSGGESSFSSSSSSSSSSSSSFSVEKLDKCVITVPAYFNDQQRQATKHAGALAGLKVLRVINEPTAAALAFGMDESTRGAEPSNKDGVIAVYDLGGGTFDVSVLDIEDGVFEVRSTNGDTFLGGDDFDLLLVQFVVDEFVASLPADAKITKETIYGDARLMQRIRDACEQCKIALSHVKETSVDIPFALEGKHHLHVLVTEDQLDAMSMPLITKTLRCVQKAIKDCDLDISKDIDEVLFVGGMTRMPRIRTEVAKLFNNGNDSAFSSARHTTKMNFSINPDESVAIGAAIQGAILSGEIKDILLLDVIPLTLGIETYGGVFAPLVPRNTTIPIKVKEVFSTGVDSQTGVDIKIYQGERTMCINNQLIGDFKLSGIPPMLKGEPQIEVTFEVDADGIINVSARERRSGVAMEISCVSKQLFGMDSTQVKSILQESAANAEMDQQVKTRVELLTKLDIITKDCLKLVNSEYLKPLLEKAGDKKSDVEQEFALLKEQLQSINKEIGHLKECSQSVALVDQLLQYDVDALKDNLMAIQQSLFTSIKQVNDMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4J8</accession>
    <name>A0A1E5R4J8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Superkiller protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3499</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4J8">
        <property type="interactions" value="540"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4J8"/>
    <dbReference type="InParanoid" id="A0A1E5R4J8"/>
    <dbReference type="OrthoDB" id="421075at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055087">
        <property type="term" value="C:Ski complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039226">
        <property type="entry name" value="Ski3/TTC37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040962">
        <property type="entry name" value="TPR_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15704">
        <property type="entry name" value="SUPERKILLER 3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15704:SF7">
        <property type="entry name" value="SUPERKILLER COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18833">
        <property type="entry name" value="TPR_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="657"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="691"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="971"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1180"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="4">
        <location>
            <position position="514"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="4">
        <location>
            <position position="1020"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ81835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1388" mass="158780" checksum="579D483AC23CA7CE" modified="2017-01-18" version="1">MSSQIKVYLKNAKEALQKKDYELSLEWCQDVLEEDASNYYAHIFIGKNNASLNRNTPALEAYLKALAIDEENLLAWKGMFQIFEAGFFDKNVLTYGHFFQLCAKYYEILLSRDXXCVELVNCIRAFRKEHETDCERDFLENMIPGQLFAETLGRHFMTPEEVLQKLLNIKIKEENSKVSTVRARNAMGLSPSDPLYNTKLNELAWEIYSKSDVGNIYAKLINVVDDDEKRTTLEEEWFHYKLKVLKSMPPGTKRSKSKELKDMVDDMIIVGHDSNEVWNYYFDWSDFRSLEDFDLNLVSTFIQKFPQDPLANILYAYICSSISKYDKVKFFKNTWGSQTENTEKXEKNGLLKEDDETAFLLETEDDCLATLLDNIEHAQTSILAHRVYANYAIAFEKYADALKYVLHGISLXMQLSRELDVDMNHTNSDMTLNLALIYTHVDSPKNHQTALQLYSKYLLEHPDSNRAKLGKALIYIEQENWGNADELLKDVVSEERDSAVALSHYSWVKMHLGELXESITNFKKALALFTGMDVPSLKIRSVNMWRLANCYIKKYEECNNSEFLDLSFKYLIESIKCTDDFAQGFSTLGSLYATYYNDPVRAFKCFHKSFXINPGDMKAAEYMVTQFTTDNQWDSAALVCQDLIKSQLVKRELQVSNWPYRVLGISYLEVQNPEDSIEWFQAALRVQPEDIESLVGLGQAYLQCGKVESALKVFNKALEIEPVHVYSRYFYAVTLGELGSFNESLAVFKDLLALPAHSQNECFHVSYAKVLVSYAETINSQGFLYKSVDFAAETIETIQHVRVSLRSNNQSLWTLLCRALKLFLSVRSKMDSLPVETIVDIFQTVNLSKVEIHESIXVDCLFDETDSLRIVAKCLVLTCKCAFAVVPFDELPRALKSLLFYNLGTVQLYAYVILQETEYRESALESFKRSIAQQSNMVEAWVGLGISCMDVSYKVSQHCFIRALSLSPKDVDIWYNIAILALKNNDHEFALEIFAKSQSIAPEQSLPRFGMALALEISGDXTLSAALFKHSYILSNGKSKIAELFYAKTVLNERLEAKNSDSRNVESEQDFVAILHCLESYLKKVPDDVFAVQCSIIALERLQNFEKAEVYVNKLVAIVEENFSQSNDEKELNHYGVIQSQFARIKLGLSQFDEAVETANLSMGILXEHKNEKVYKDAIVSNYCVLGLAHFFQNEHEKALEYFQNVLNANDEQNSELVGLVCKALYGVGNDDFKELALSELTDFIGANGFTLKMAILIASITLLEENNGPQELKELLKEVSGMIYDTSDHHMDMAYLIDQVNRRLGTDGQLRQQSLQRAXFLTPQNVNVWKLMDNKITYKIGLNNYKQINVVQKSNELARLGNLSKVQRAMFLCPWNEEAVNTLKYCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4R1</accession>
    <name>A0A1E5R4R1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Myb-like DNA-binding protein BAS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3556</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4R1"/>
    <dbReference type="FunCoup" id="A0A1E5R4R1">
        <property type="interactions" value="1835"/>
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    <dbReference type="STRING" id="56408.A0A1E5R4R1"/>
    <dbReference type="InParanoid" id="A0A1E5R4R1"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019185">
        <property type="term" value="C:snRNA-activating protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="P:snRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="Homeodomain-like_sf"/>
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        <property type="entry name" value="Myb-like_DNA-bd"/>
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        <property type="entry name" value="Myb_dom"/>
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        <property type="entry name" value="SANT_dom"/>
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        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
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        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
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        <property type="entry name" value="Myb_DNA-binding"/>
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        <property type="match status" value="3"/>
    </dbReference>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="50"/>
            <end position="139"/>
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    <feature type="domain" description="HTH myb-type" evidence="8">
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    <feature type="domain" description="Myb-like" evidence="6">
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            <begin position="140"/>
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        </location>
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    <feature type="domain" description="SANT" evidence="7">
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            <begin position="143"/>
            <end position="186"/>
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    <feature type="domain" description="Myb-like" evidence="6">
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            <end position="244"/>
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    <feature type="domain" description="HTH myb-type" evidence="8">
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            <end position="248"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="5">
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    <feature type="region of interest" description="Disordered" evidence="5">
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            <begin position="962"/>
            <end position="985"/>
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        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
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    <feature type="compositionally biased region" description="Polar residues" evidence="5">
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            <begin position="750"/>
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            <dbReference type="ARBA" id="ARBA00023125"/>
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        </source>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ81874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1093" mass="122219" checksum="FE911F9AF0DA3AAA" modified="2017-01-18" version="1">MRSESSINGAVSDHSDSKIAAHKSKHKKEKKKYKTLNALAITAKLGITTHSPKQRIPWTEEEDAKISAYVQAFLYNIGHKNGIEDLQIESAKLLQNLDWDDLAAKNFYEDSLNVNGSDSTGIRKKRKGNDIKKRWYSYLDPTLRRGRWTPEEDELLLKAYAKHGQHWALIAKDVPRRNLDQCSKRYHEALDPNKSQGRLRAWSEEEDLQLISRIQEYGTKWKSISMGMEGRPSLTCRNRWRKILTSVVRKKASETITKAVQDAGNINDLSKLSTMTDDSATPNSDQLKESSQINFRNDAESDYHSTSERAAHFHQKNMNTTSQRTALSPNFSAPNTERSSMLPPMPNLNNFAHAGQKPFQGTKFPTSPLTLGSPSTLPLLDVLNGAKNPHAGLANLDKSQVMLLDKSPSPDISMDFHHSLTRSTSNIRSTASAIPNAQPNQNSLNSFHEFSPHLLHHKSHSGTPAVDDQTDFMNHVSLLGEKNGHQNTRNAFSNAPSNVYSPAISFSDERHFSHDNLKTDEHSGTSLQAQVVQNHRDSSETRNLPELNNKREYEHQHGDHHDAHTVDYDNNFFAVNANGKKLDTANYENLSPPTNLADGNVHSFPKPHYQSTIDLPSQTKPQTHPVLSPTSSLPAQSIKHTEWKFSLKNKGYTLSSGVISSTELVEQLIEQAKKNGLKISIHQHIHNHYNPGNNTSEFLLNQNKHGHNKSHFSNATSSDGSLNSPNHMRFSNATQSSSNSNKSSGMKNNIGETGLSNDLFQNTSTHPMNQQPSTTSPNSSAMTTPKVIQDLTEIGHNRFKHFKTLNPTVRPKLGSSSSQNDFQKILHQSTATGSLRKHKKIRRNQNLNGAVSSGGEVSSAESSNVSTPGNNQLDPNIQKNFTKRSGNKEGFHDSIQYSSRRFNNIQSPSPALNEHQKNSPQSSEQHHSIQNGVAYDHISKDSKFSNRISAPPIDTVPQESQHFSHFSVHSASQSDQQKNDLKEEEFDDDDQFDFFQSLRALQPLSVPNTANLDMMFGSTRMIDTNPSSVANNTGTDRVQSHLANALTSIQREEINQRSYHKSAVGHQELYENLYEAPEESDSEQGNAWFFNPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4X3</accession>
    <name>A0A1E5R4X3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS9m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">37S ribosomal protein S9, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3732</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4X3"/>
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        <property type="interactions" value="207"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4X3"/>
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    <dbReference type="OrthoDB" id="10254627at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="30S ribosomal protein S9"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023035">
        <property type="entry name" value="Ribosomal_uS9_bac/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_uS9_CS"/>
    </dbReference>
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        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
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        <property type="entry name" value="PRK00132.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US9M"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6 7" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ81967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="308" mass="34800" checksum="ED1DF54D5A96B834" modified="2017-01-18" version="1">MLKRYITGGNVATSYTKLTSQLPRFCRFQSTKSSESTTGTSNNAVITPVDATRVAVSPLKNFDAASRLLPTLPTFYTQNPPHEQNMSELRYMMSKYGGLPKQELHLVERKQWMARGQYALNGGGTRLKSKEYSELVKGLVSLNEIHPQFKNDELLSFLNRFIRKSNILENPKKLKELDHLGRAVARGTRKSSTAKVYVAKGDGQVLVNNQRSLNNYFVKMKDRESIMYPLKVIGQLGAFNVYITCSGGGVTGQAEAAMHALAKALLIFNPLWKTRLFKAKLLTKDYRHVERKKPGKVKARKMPTWVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4Y2</accession>
    <name>A0A1E5R4Y2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein ILM1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3537</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4Y2"/>
    <dbReference type="FunCoup" id="A0A1E5R4Y2">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R4Y2"/>
    <dbReference type="OrthoDB" id="3970994at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018815">
        <property type="entry name" value="Incr_loss_mito_DNA_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28029">
        <property type="entry name" value="PROTEIN ILM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28029:SF1">
        <property type="entry name" value="PROTEIN ILM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10311">
        <property type="entry name" value="Ilm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ81954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="181" mass="21040" checksum="801E15EB63486EFF" modified="2017-01-18" version="1">MAIISSSKVLFVRIAFLFALSYYCLKDVEFILRNSTLVAFSHSMNLPQLVINKHSSQLGLFSLLFFFAGLHDLIPLLEKNHTYFLSIVPTRLTFFFIVTGLSFYQEHNLYLHNNAVFAYGFIEIWTNFLIFTSLREERNTRFEEKERVLAADYYKYDGNTPADQQELAEDASTFPEEAEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R527</accession>
    <name>A0A1E5R527_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Nucleoporin NUP57</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3644</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Nucleoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R527">
        <property type="interactions" value="278"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R527"/>
    <dbReference type="InParanoid" id="A0A1E5R527"/>
    <dbReference type="OrthoDB" id="6162375at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044613">
        <property type="term" value="C:nuclear pore central transport channel"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036228">
        <property type="term" value="P:protein localization to nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025574">
        <property type="entry name" value="Nucleoporin_FG_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024864">
        <property type="entry name" value="Nup54/Nup57/Nup44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025712">
        <property type="entry name" value="Nup54_alpha-helical_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13000">
        <property type="entry name" value="NUCLEOPORIN P54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13000:SF0">
        <property type="entry name" value="NUCLEOPORIN P54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13634">
        <property type="entry name" value="Nucleoporin_FG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13874">
        <property type="entry name" value="Nup54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup54 alpha-helical" evidence="9">
        <location>
            <begin position="314"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="26"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="134"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="525"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="83"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="186"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="534"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ82010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="606" mass="64963" checksum="2C5660DA45C2BE03" modified="2017-01-18" version="1">MFGNSGTSGSSGFSFGAGAGNTGTGNTSASGGMFGKPTTTPGASTGGGLFGQQNATNTSTATGGLFGNNNTNAGAAPSGGLFGKPTGTTTSMTSTAPTGGLFGNTGANTGTTAANSSTVGGGLFGNNNANPTSTGGGLFGNKPTTTNSTGGGLFGASTASNTGTGLFGKPTVSSANNNLXGAGLFGQQNQQNQPASGAPQQPMQSQTGGIFGKTTQPSFAWSNNNAHLQQQQQQQNSLLQQQQQQHLQQQQQQQLQQKQSTQYSANYPQQIQEQLLKLQQQWLPVAENCKFKGFVYNKMDTSQQAVLDLQKPSNAITQNEWEQALKDRPGEEYLPLLIKSPTDLQERIALQTKTVAQYRQIMNTVIYEQHLSQLKDKHNLDTTIRLSKCQTKLRDLESRLWKLNCTIKTILEQSKNYKFEINSDALHSDYLDNHIHAGNKGDSNYNNDSKIHGASSSHYSNNMFGMPVSGIDNNRVPLDEYVQHLVKQSEDPSSLGKVQSGELWARLSLLKERCKVVLERLDGTLGSQNGDLGNKGEEKLDREDGHEEEEEEEGHDARNDEAKSLEANIDAKLASVLNVQQDGLMYLNKVLLQDLASLKELNSSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5E5</accession>
    <name>A0A1E5R5E5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3621</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R5E5"/>
    <dbReference type="STRING" id="56408.A0A1E5R5E5"/>
    <dbReference type="InParanoid" id="A0A1E5R5E5"/>
    <dbReference type="OrthoDB" id="5239630at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019034">
        <property type="entry name" value="UPF0390"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09495">
        <property type="entry name" value="DUF2462"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="93" mass="10497" checksum="8B0D4A0B2E025F8B" modified="2017-01-18" version="1">MPQASGKLNKNKKGHRVTKRQTNLRKAAPLQLKPKSVHKGLHLHKLSSKYSITEQTEKLVASKVGHLEVLKGTRKEIEKQKKQKALKDAKKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5G9</accession>
    <name>A0A1E5R5G9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Peflin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3627</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5G9">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5G9"/>
    <dbReference type="InParanoid" id="A0A1E5R5G9"/>
    <dbReference type="OrthoDB" id="186625at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051426">
        <property type="entry name" value="Peflin/Sorcin_CaBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212:SF3">
        <property type="entry name" value="GH27120P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212">
        <property type="entry name" value="PEFLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="228"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="295"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="157"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="42"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="99"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="120"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="157"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="8">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ82155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="403" mass="45588" checksum="E233FC478706613D" modified="2017-01-18" version="1">MGKKVNYXAGDNLPLYATPEQSLEYGKQWEEGKLEEQRRQRLSQNLMMQRQQQQQRKEKLANMPESXSFPAQGSPGSGNRHSSGVSGGQRRPVPSNLMPNNFPQQNYQQPPVTRPLPVVPAQQQSQQQPQQQXQYAPQYAPQYLPQQQMQQIXNVHGQQPSPMAQQHYVSKPQHQKQHRAQQPGTPTSGISQASPSGVSFSSAHARLGVVPGSSNRSSSSSLNGDKQDVKEAASVLFHKYDLTKSGRLGANELQRLLQNDDTTPFSYSSVDSIINLFGVSRSGTLNLNEFIYMYHKIKTWRDVYEQTDANRSHTLTSLEFSEAVQKMGYKVPIEVVENLFDQFAEYDNGIKCLKFGKFVESTVWLIRLTKVFRKFDEKSNGVAVIDYKDFIDVSLYLGKFLPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7H8</accession>
    <name>A0A1E5R7H8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">U1 small nuclear ribonucleoprotein component PRP42</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3216</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7H8">
        <property type="interactions" value="348"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7H8"/>
    <dbReference type="InParanoid" id="A0A1E5R7H8"/>
    <dbReference type="OrthoDB" id="10265668at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030627">
        <property type="term" value="F:pre-mRNA 5'-splice site binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000395">
        <property type="term" value="P:mRNA 5'-splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059164">
        <property type="entry name" value="HAT_PRP39_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204">
        <property type="entry name" value="PRE-MRNA PROCESSING PROTEIN PRP39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204:SF23">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN COMPONENT PRP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23241">
        <property type="entry name" value="HAT_PRP39_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23240">
        <property type="entry name" value="HAT_PRP39_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ82865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="536" mass="64119" checksum="93C71F445F810BE8" modified="2017-01-18" version="1">MENDTYFVKYSTQLSANPLKVSNWELLLNHLIATYATPLDKTVNASTISLISATYKSMLVHFPYLENYHIDYALFHFRLGGIKQARQIFLNALNTQNNRSLLIWCEYLKFLMKIEANKKTVLKNFEKAEFHVGMHFFASPFWDLYLQFILQNGYSREIYLRVLRKIIEIPQYEFAKYYTQWFEAIENEIKDIKELQKFVNLQSINKKYNINLHDIMNSNIRKGHLLQDLRLKLLSCFKELYQAIEYQVFEXYQLFELPLTMYKKTNQCYYIPSNIPLSSKFLTNWLRYLDFTIKSHNNQLILIVFQRCLNSANIAHLDTIWLKFADWLIQNNSSNGQSDYLGALDVLKQSQKFLRSPISNLKIVCKMSDLYLKLNYSSQSLVELWHAHYGDAASHPDYRIFIKYYSYMRFVKQPVSEIRIQQRLKEFAEMNFLECLGDKNYMEYIIKLKLKKLLRLGKFWYLYLHLIWNTEKSTETKTKSHTEKSKIIGSLLPVVMENLKKYNRKNQLTKIKNYRVVEFWLSKYSPEHLFLFKSLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7L6</accession>
    <name>A0A1E5R7L6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA mismatch repair protein MLH3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3435</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7L6">
        <property type="interactions" value="490"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7L6"/>
    <dbReference type="InParanoid" id="A0A1E5R7L6"/>
    <dbReference type="OrthoDB" id="429932at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032300">
        <property type="term" value="C:mismatch repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.20">
        <property type="entry name" value="MutL, C-terminal domain, dimerisation subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.100">
        <property type="entry name" value="MutL, C-terminal domain, regulatory subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014790">
        <property type="entry name" value="MutL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042120">
        <property type="entry name" value="MutL_C_dimsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042121">
        <property type="entry name" value="MutL_C_regsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037198">
        <property type="entry name" value="MutL_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH, PMS, MUTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073:SF47">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08676">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00853">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118116">
        <property type="entry name" value="DNA mismatch repair protein MutL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MutL C-terminal dimerisation" evidence="2">
        <location>
            <begin position="490"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="733" mass="83610" checksum="4542508EAFE8C523" modified="2017-01-18" version="1">MSIQPLSEKDQNLLRSSVLIPNFDAIVRGLIANSLDSNASHINLSLDIESFTTLLVYDNGIGILPECLPGIGVVNHATSKHHNFAATETQNTYGFKGCFLFSLSSCCTSLEVISKRKAYNSVWKFDTLEKKNTMISPNNLIFHESGTLLKVNNIYSKFPVRLKIMKSKPKHQIVQNLKQIIFDLMVTYKEFKKAIVINVEFINSNLSDLSYTFGFKQSSSTNIIANIFNEVFPNVLETSFLKKVSTTFKNFKIEGTLSTRGLPTTTYQYIFFNKFPFKHNQDIQEWFKKANMMFENMNNFDSSKFLTKSVGNPFRSFPVIILVIHSKTRVADILNHLDPETEYLSDHYDINIIVSILDKILESFIKHLSLTSGQECAPVKEHSKTKLQTKLYAEKASINMVLNSKVKRACLDFKEIEGTITKSFNNHASENKVNGTRRLSYNINKAILSSTGLPSLSKKLRLSIPSVTPPILDNIHELXLKKSDISNAFVINQIGEKFILVKLRSENNKLCIIDQHAADERIKLESFLFEFICRPIPLAQLSQSHQAQESQLLLLEHFSAHEVSLFEQFYSDFLRVGIQYTLLSENTLKIKSLPTILCQKIKTRVGGGEITYDLDFLRNGLLQHAYDLDNGNKRKFRKQKMENSKGQNWWLQTQSIPTVLLELFHSKACRSAIMFGQKLTLKDCETLVSNLSQCHMPFHCAHGRPSITPLXDFAKGSSNLTFRHEFFEKDYEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7S6</accession>
    <name>A0A1E5R7S6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RecQ-mediated genome instability protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3230</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7S6"/>
    <dbReference type="FunCoup" id="A0A1E5R7S6">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7S6"/>
    <dbReference type="InParanoid" id="A0A1E5R7S6"/>
    <dbReference type="OrthoDB" id="3971164at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.770">
        <property type="entry name" value="RecQ-mediated genome instability protein Rmi1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042470">
        <property type="entry name" value="RMI1_N_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013894">
        <property type="entry name" value="RMI1_OB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08585">
        <property type="entry name" value="RMI1_N_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecQ mediated genome instability protein 1 OB-fold" evidence="2">
        <location>
            <begin position="59"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="134"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="275" mass="31601" checksum="05DD1BE81527F9E3" modified="2017-01-18" version="1">MSQSALSSDITAYVRATQSYKNDKEQAFYESYQNISFTKYISNYIATKRQLKAHKNMTSIQDVLMLGIDQDFLFQIVIVEDINSSLLQQLDDLEANLNPMNIRVDRATNSVRNQNNKKKTLVSSIDDAADEENTASSDTTNNANYKSNNDSYNTLRSKGDKVYKITLQDKQGNMYYAMLNEYSSSLSYQLTDPSQPSGFRPVLGSKIIIKSNTLFNRGMFHLSKNTTYFYLGSESSIKKWNENFNSKMLHYWKHKLIDNGQQAEPSNGKKRVYEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7Z4</accession>
    <name>A0A1E5R7Z4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Mitochondrial 2-oxodicarboxylate carrier 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g3391</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7Z4"/>
    <dbReference type="FunCoup" id="A0A1E5R7Z4">
        <property type="interactions" value="236"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R7Z4"/>
    <dbReference type="OrthoDB" id="434783at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051752">
        <property type="entry name" value="Mito_2-oxodicarb_carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356:SF1">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="11"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="112"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="222"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ83008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="317" mass="35313" checksum="D9324687C605EDF4" modified="2017-01-18" version="1">MTKFVKTTNDLPFTYTILAGAGAGISELLCLYPLDLIKTRMQLVTKSSSQQYKGTFDAFSKIIKNEGFLKLYSGISSPIVMEAFKRATKFWAQTESSKLIVKNLYRGNIKGHESKVATLSGLSAGIVESFVVTPFELVKVRLQDVNSTYKGPVDVLKSIVYEKSHTSVSEMPTQVKSSFHPLKLFNGLEATMWRHGLWNMGYFSIIFQCRTLLNSWHITKDNKKTNSLISGCVGGAVGSVMSTPFDVAKSRIQNAAKGSPLAQKWTLKLVLEIYQKEGFRSLYLGFTPKVLRYLPGGGILLVAYDQLMGMFLQFHQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R810</accession>
    <name>A0A1E5R810_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Dipeptidyl aminopeptidase A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3384</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S9B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R810">
        <property type="interactions" value="311"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R810"/>
    <dbReference type="InParanoid" id="A0A1E5R810"/>
    <dbReference type="OrthoDB" id="16520at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000003">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF160">
        <property type="entry name" value="DIPEPTIDYL AMINOPEPTIDASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="10">
        <location>
            <begin position="204"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="9">
        <location>
            <begin position="684"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="11">
        <location>
            <position position="888"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ83024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="891" mass="102902" checksum="7047F0641287BC17" modified="2017-01-18" version="1">MQQQRKKKDETDLELHDLENQLHSTRHASAGNHQLQQKGVSLQSTFPNSDHVSKSAPVFITTYFNKHLKQRKLTFLYAILVLALFIYFSIALPWPNHHNPKSSSFPTVVPPEKRFNVENLFNGDFDIHHEPFKFIRAPPDSVRHVEDPGMFFTLSSAEKKGNENGXQKILVRNMFDLQWSEEIGETHFTYNDIEYTTQGIEISYNLDKMLVMTNKTSLFRHSKTALFWVKDLKTNTYTPLSEKLLRNAQFSFCFNFISSVDLNYNIQIYPVHGGNTLRPXDITTDGKDKAVLNGIADWVYQEEXFGTDSTIWWSPKDDQFVYMKTFEQNVEVYKYPFYIXNYQHFQEFNYPRPGGKIPEYELHVVSIVTGQVFVLDKYQYKEELVYDVHWVDSDRVIVKKADRISNELRYILHSFNHTSSQWEKSIFNHINFDQTYNGWVEKQQPMLNIPIYGSAHSDLLDIGIADIRPDSNGFNHIFYTPSIKEPTLFMXLTRGTFEVQKLLGFDKDTNQIFFTSNSKHPMARHLHAVSILDFDDEGIQDLQKGNDVMEYNDFLLSQSCRWGLRKILGPQLPKGWAGPISKIVTAAGMENFDGDGLLLSDISHIEKTLNEKFILPKVNYQSMEILDNADFSQNDNEEGSITIHYKEYLPPGFDSHSSKLHPILVHVYGGPGSVAFTTQFDVFFESAVSSSLDAVVLEIEPRGTGNKGWEFKRYANNHIGYWEPRDIQSVVQKYIANNKNKIDTDRVAIWGWSYGGFTTLKTLEHDKGHTFKYGLAVAPVTNWSYYDAIYSERYLGLPGPSYNDAVISNIDSFKEIKRFLLMSGTADDNVHVFNVYRLLDSLNLHGVTNYDMQIFPDSDHNIAFHNATKVIYSKLFFWLQNAFXSKFDELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA22</accession>
    <name>A0A1E5RA22_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Altered inheritance rate of mitochondria protein 29</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2544</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0538 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RA22"/>
    <dbReference type="FunCoup" id="A0A1E5RA22">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RA22"/>
    <dbReference type="OrthoDB" id="937at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018794">
        <property type="entry name" value="UPF0538"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18444">
        <property type="entry name" value="UPF0538 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18444:SF9">
        <property type="entry name" value="UPF0538 PROTEIN C2ORF76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10209">
        <property type="entry name" value="DUF2340"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="147" mass="17336" checksum="60C452A60CAAFC6A" modified="2017-01-18" version="1">MNVQDYDQEEPLTTSMKPLSMCTLTVRVIKSFPYRNVKPIVLTNYDITSKSAKDLYEEVVNHISTNGAFRPFRNVQYDHMKVYTHAHKSKTVNLVINFDHDDDPSWVLDITDENKKLSHYDIANETEISLYRLEDYENFKANPEEKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA28</accession>
    <name>A0A1E5RA28_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquitin-conjugating enzyme E2 6</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">E2 ubiquitin-conjugating enzyme 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Ubiquitin carrier protein UBC6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Ubiquitin-protein ligase UBC6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g3001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="15">Protein modification.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA28">
        <property type="interactions" value="973"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA28"/>
    <dbReference type="InParanoid" id="A0A1E5RA28"/>
    <dbReference type="OrthoDB" id="1158011at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23799">
        <property type="entry name" value="UBCc_UBE2J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000023">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 J2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="17">
        <location>
            <begin position="5"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="196"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00043952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ83737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="263" mass="29856" checksum="31B1796016B8E70C" modified="2017-01-18" version="1">MATIQANKRLTKEYKNIVANPPQYIIARPNEDNILEWHYVISGPPDTPFVGGEYHGTLVFPADYPFKPPAIRMITPNGRFKENTRLCLSMSDFHPDSWNPTWSVSTILTGLLSFMTGDESTTGSIVTSDEVKKRLALQSKHYNSRQNTRFKKVFPELVEAYEEELKQMEENSKDGENNAGEDFKMXFLGIDQQNELESRKQMERAKNEKVVPLNEIEDPEDRIRAAELASGNSNSSSNSKVLNFFSMAVAFASLAFLCKYLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAA6</accession>
    <name>A0A1E5RAA6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2827</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAA6"/>
    <dbReference type="InParanoid" id="A0A1E5RAA6"/>
    <dbReference type="OrthoDB" id="762982at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF90">
        <property type="entry name" value="CENTROMERE DNA-BINDING PROTEIN COMPLEX CBF3 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00755">
        <property type="entry name" value="AFLATOXINBRP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="33"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="74"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="206"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="78"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="94"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="221"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="266"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="5">
        <location>
            <position position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ83839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1065" mass="121229" checksum="EAA2B96B1B0CFF64" modified="2017-01-18" version="1">MSKLIPIKDEAALVLEDGSIYKIQHKRQRKVLSCVRCHNKKIKCDKQRPXCTNCLKNNMMCSYFVNNRVSGTSLAERTSSSSGPSDSTTSSVSNVEFGTFENNRGNDIRQGSLDSMNXYSEKSFSNGETHIEQNLKERSNSLPSTVEMSVGIARNNHSIANSCDSAITTNNQKKLEILAAIEEAKKFQEKERQMIAFSKKNKRIYTKKNGESNGGLVKRVPKCENTKHEKXPTSVSFSSSLSSPLLSSSSSFSSTLKKTPSKKNKQKESGSHACSKTETDCSLHPTKLKAVEIKNLSSDLPTENVSLLLFKRFMKSVHPVIPLLNVKNYPKHLSEFYNDLNHKKSVDYKFLINLLLINYTALRAEYYDSSVSGKNMNLLEQMIDHHKNQIDDLLQENSLIPILTKFTVEVMINSVLENPNFTKIAELVRVSQKLKLKHGLLKLIQSTQLSPDENNDQRLQIKERTLLMWQLFRLDTLTSLHNDLPPLIKFMDISFLDEVDELGNFDPSMCFLNAKFRFISLINDFCTHFKTGQGIDSLKERIVELHSCCTNARFNLSNHKQGLLELFDESNDDGNIKDKLNFIDWAIYNVQTFPDRALLLLHLNLIRLSMPVLGERKNKRLVEFGEHFLSIQNILNDSGNLQLDGSKVYDYEDLTNNLIPSSLHYLDSFNKITDLFVNEGWGSFNWELSSLPIDAITFTLKTLTLDLNKHAANDQCYNLNNDVRFLLLKKCIPRVEMVLEIEQSNTTRSKHCVCLVCFHLVKLLFDLIKIKFGLVKHYHNEESSCEFFFNKYDIEQQNIFNKGIISMSPLIPQPAPAPFVASEDHSHGNSSLLSDPVKNSSIFASSTTTSSSTATNTPFTNNAVYSEPQQQSYNEPNFEKELFSSDMQQQLEQHVRRKSPLIKDMLNIPACTNTEYYETPIRISPLPTLLQQKQHFQASEGSPSIAASIHQCSNTEQNVEAFAFSKKTKSQVDQISSCYPKTFPDDYYNTMDLETRVKKLNDIRLSVERYIXLLNEKSANTNSYSHSPHAYSLSAIDNFEYDDYYIEFENAILEILSGILSMPID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAC0</accession>
    <name>A0A1E5RAC0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2766</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RAC0"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="309"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="576"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="605" mass="69023" checksum="D608994043DE1E7B" modified="2017-01-18" version="1">MVHYKTKFLLNCFHFWLLLPVFLFISTVWLKHSVLKNICEDVIQINKSTVDVTTKVSTETLQPTVSKEVSFKSTIASTFTSPITVTTTQIAKPTVATVPQAYLQETIWLQINSAVKKVNTDLLQHQYVPHINSLIMAKSALYNQTTEEFELVLNNIQKNISDMLANTSAEIAHTVETLSNEGTLLSSGNNENLSLDSLKIKADMFDSMFQSQKTFLSREQSRLLNFNMTNFKQGKNSSTIPKFALNTTQTNLLNDIVNXYVCNNTFHFTKDEQVSSENLKRELHTLDVSQNTTKNIYSMSKSVESSQRYCVALTVIFVTVFIVIIIISNYMKWRNIQTNEQNHFTAVLNQTPKKEKCSVNTFNMHYKLSNANKLKTKSFTWHILNMISLCKGLIHTLFILITEYMLVIGENNMGFLKTVSSDNTPASTFTGINTLNVEKKTLVQKSFNTTSTAQSLGELNSNLNQNFNPFVYYNXWDTEHNNNTIMTAYFTSIGALNVTQALLEYRNELAGDRNYLGIPAINIVFDSDTIIDNTIKKNGTIFKRHQLALKDPYSNYDTVNMKISWYSDLRSQIQNCLLMLIFTTSLMLLISFIVYFVDHIYVTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAC3</accession>
    <name>A0A1E5RAC3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Arrestin-related trafficking adapter 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3109</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAC3">
        <property type="interactions" value="87"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAC3"/>
    <dbReference type="InParanoid" id="A0A1E5RAC3"/>
    <dbReference type="OrthoDB" id="2238745at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF174">
        <property type="entry name" value="ARRESTIN-RELATED TRAFFICKING ADAPTER 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="2">
        <location>
            <begin position="398"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="698"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="837"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1021"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="211"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="904"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="958"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1026"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1052"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1082"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1111"/>
            <end position="1143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ83821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1240" mass="138189" checksum="1D8235EEB41C1182" modified="2017-01-18" version="1">MPTTFPDRKQNLNRKQSMQSFESNELKDAPVLPRDRNILELLKKEKNLQTLHSSASLKIFLVLAEDKVFLQGFNKSQWEQKNPAMLRGDLIVKIMKPIKLKSVTLKFDGYSITEWPEGMPSLHNSKKPSGEYSEMKDIVHHAWPFYKHDDMPTKHHKTVNSASFLKSCSGCNMFYPLDIIESNGSNVRLSRTLSNASSIGDDFNQHGLADNNNNNNNNNNSSGNHLASNNNNGSHQNKHQQQSHRSLSPLGGFFGGMTRRRTSTVTSMDSFNLSEMLSSTLSNNSTGGNDTLVNGLQGGNSSSGAIANQLISSTNGVGPNFIFQPGYYVYSFEQCVPLSYPESIQSTFGHVSYYLNLDVERVGAFKSNIHAKREIQLIRTPSDSSVEETEPISIVRDWENALHYDILIASKDIVLNAFLPISFCFTPIDKVNLHRIRVYLTETMEYYAKGKKVHRLEPTKKILLSEYKAPPLKGLSPEAPKTKAKNLGNILSDNPADPKNGYIAYKEIDLQVYIPEFLNANTRIHPDTACQQIKSNHWIKLGLRLSRYVDGKLKHFEISIDSPIHVLGKYCTHGNTLLPSYESHMAFAEALGPPPPPSTLFNQNDDVSYNLYDMNSMHLDNHQDDESSSFSNNAANALSGLENFGTLNVSRRGSVATDILSDSNTSNTINLGYHTSNIYFPKEIVNSPMMSPNVEPLNETSAHHHADNHRRNSVHTGMDATSPAVRPLRSETTSAIIPLRKTRSKTLTSNHQPTFKHAXTFAAPMNKSMSLEQMNQIPEMQSNIYSPDKLMPEFMSPQALPFGSPLSSPVMGPIQFNNKYLSPSASFVNLNEDLIHSDVPPPPFEERESKNKTKRKDSRNSSIVGFSQLPESAAGSHGSLESHMKNHPPSYMQVLKNDGIVDDSSLNNNQTSEHASHPRGIHSERNHHEXHKNVPQGKPTAGNTSIPALKFTSDGDSKNNVISNSKHQVPHLNRKENVDPATPQEEDFGSAFMFRKLLPNPSQQNGYNDISALLLDDQENDEIDPKQPLSPKSSPHLLSVNKGSTAIPSLKYEGEDSHMKRSSQEKHDRNEFGDDIADIRVRSSSSSPVRSLLQDNHETTNTPAKKKKDMSNSANASPFTEKTRNTLNGNTKNPDESFTSSSELGDDDVDDYEGDGLTKDIMPLRTSTDLQLRVPLLNGLSRQNTQQSTNNPFSNVFSESKESVLLSNFKAQNTDIDLKTLVQRNIQTPKQAHFEKRGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAJ2</accession>
    <name>A0A1E5RAJ2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cytochrome c lysine N-methyltransferase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2712</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAJ2">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAJ2"/>
    <dbReference type="InParanoid" id="A0A1E5RAJ2"/>
    <dbReference type="OrthoDB" id="441812at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF34">
        <property type="entry name" value="N-LYSINE METHYLTRANSFERASE SETD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ83926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="549" mass="63842" checksum="E07886B006630586" modified="2017-01-18" version="1">MEFLSLLKERYANEFQLNSNVVVKPTKYKGGGLGVFYKHTTDLLALETIELMRIKRKFVINLPEFQEHGQHFPLFSTAMNELMSDPTFDFVSKYASEKNILMFCLTIFLSQVKDPDSDAFLIEDTERVDLKLAKEYADFVQKYISLVLMETSISTPLLYSKENLKWYKTLYPFLAVALEQQQSFLQELHVFFESKLHINVALSDLYQLYSAIGSRSLQIPEEISSVTQDFQVSETLVPVLDFVNHDNFKKNAYFDIDRSTNDIVLFLDVPAVADKLASFEEFEVYISYDPEEHLNKFDFTYGFVPPFSLIANSESQYLDIAIDFDLFPAEYRNISKWFQASPLLKFKLLPNKEVQVIDDEEEGKVGSLTIFTILSGAEDYKFVLNSDCPFQLEQLANENNXPNPAMFAQSVMDAMETTESEDYENTEIIAPQLRYFFQYKETPKINLSNLHEIASRLGVTKHSKVEWIDSYLKPYCVERCKFLKSRLDIPLVSAEYYFLKRVITNFNSDNLRTSQDKKLTENQFLDLLSTLQEQNTPKSLFLAWYERFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAJ4</accession>
    <name>A0A1E5RAJ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein FMP42</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2787</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC43A transporter (TC 2.A.1.44) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAJ4"/>
    <dbReference type="FunCoup" id="A0A1E5RAJ4">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAJ4"/>
    <dbReference type="InParanoid" id="A0A1E5RAJ4"/>
    <dbReference type="OrthoDB" id="330047at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052599">
        <property type="entry name" value="SLC43A_AATransporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20772">
        <property type="entry name" value="PROTEIN FMP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20772:SF2">
        <property type="entry name" value="PROTEIN FMP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="169"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="364"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="391"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="444"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="485"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="262"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="267"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ83891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="537" mass="59176" checksum="D78354117B2DBBD1" modified="2017-01-18" version="1">MGLKINLPLLQICCAVIWCLVAAGPIFGFAALKPILVQEGVYADLCAPPSINVTYTPTSNIKHAFTSLSQVVPIKPCDKQDLKLNKIFTIGAVVTNLCSFLVGFVLDKYGPRASGVIGSVFIALGSLVLAYSKSITTFDPYITGYVTLAIGGPFVFISCFQLANSFPQYSGSILALISGAFDTSSALFLFYRLYYQNYNPEYNLKKFFLGYLVVPLFILLCQIFIMPKESYKSISAINKLFQEGLDEEGFVPQNAINQEQDTHERSTLLSSNSQGLHPTASTASGRRKSVLEVDVENKLVDKTQGIFGIMHEYSAIDQIKSPWFILMQVFTILCMLRINYFVATVRSQEEYLLGDYQLALKMNSIFDISLPIGGVIAIPIVGILLDHTPTLYVLCILFGTSVIVGACGLVSSFVINLVGILLLVAYRPFYYTVVSDYCSKVFGFDTFGTVYGLLMTLAGLCNLLQTPLDYWTHTVFNKNPTPVNSILLLLTVVFGLWLLYYVKTHAVSKKVKNFAAIEEEDEAVSEDSTPTTAYGSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAL6</accession>
    <name>A0A1E5RAL6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein MMF1, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2743</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RutC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAL6"/>
    <dbReference type="FunCoup" id="A0A1E5RAL6">
        <property type="interactions" value="514"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAL6"/>
    <dbReference type="InParanoid" id="A0A1E5RAL6"/>
    <dbReference type="OrthoDB" id="309640at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019239">
        <property type="term" value="F:deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00448">
        <property type="entry name" value="YjgF_YER057c_UK114_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.40:FF:000001">
        <property type="entry name" value="L-PSP family endoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="entry name" value="RutC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006056">
        <property type="entry name" value="RidA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00004">
        <property type="entry name" value="Rid family detoxifying hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803">
        <property type="entry name" value="2-IMINOBUTANOATE/2-IMINOPROPANOATE DEAMINASE RIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803:SF58">
        <property type="entry name" value="PROTEIN HMF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="YjgF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="147" mass="16284" checksum="F0F0ECF632B4CE05" modified="2017-01-18" version="1">MESSKNFARSASKTNFVRFQSTLKPVRTELAPPPAASYSQAMKVNNLIYVSGQIPLTKENKPVEGDIKVKAEQVIGNINEILKASNSSLNHIVKVNIFLTDINNFADFNSVYAKYFNVHKPARSCVAVKSLPLNMDLEVEVVAVEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAU1</accession>
    <name>A0A1E5RAU1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein SNA4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2919</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0057 (PMP3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAU1"/>
    <dbReference type="FunCoup" id="A0A1E5RAU1">
        <property type="interactions" value="349"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAU1"/>
    <dbReference type="InParanoid" id="A0A1E5RAU1"/>
    <dbReference type="OrthoDB" id="2802411at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000612">
        <property type="entry name" value="PMP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659">
        <property type="entry name" value="HYDROPHOBIC PROTEIN RCI2 LOW TEMPERATURE AND SALT RESPONSIVE PROTEIN LTI6 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659:SF40">
        <property type="entry name" value="PHOSPHATIDYLSERINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01679">
        <property type="entry name" value="Pmp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="73"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="85"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="109"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ84012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="137" mass="15513" checksum="E41AFFD6096AC378" modified="2017-01-18" version="1">MCCTLLLIICAIIFPPFPVLLVKGLCSSDFLLNVLLTLLGYIPGLVHSLIIIFERQQSRNMDRWYYQQGWTARERLSQSRGSPATNNTGNNTSNSNTNPPQPCEHHHYYQQQQPQPSQQPNEESNLISKGSPPPYTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAZ1</accession>
    <name>A0A1E5RAZ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Putative oligopeptide transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2733</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAZ1">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RAZ1"/>
    <dbReference type="OrthoDB" id="9986677at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004648">
        <property type="entry name" value="Oligpept_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22601">
        <property type="entry name" value="ISP4 LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="191"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="255"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="333"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="397"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="454"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="482"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="522"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="567"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="634"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="712"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ84070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="774" mass="87420" checksum="9DE50F27562EA0DF" modified="2017-01-18" version="1">MSGDSVQEKYWKSSASQDDSIKEITEDSSNKEVKRVEEFECADLSSDSQSEENFNEINFADVNDIITLGECTEEEYDALPSFTIRMSVIAVVVSAVITAIDIFFSMRFPAISIGPIVAQMVSYPMGVAWAKIMPNWKIKVWKDTHVYLNPGPFNKKEHTLIYIFCNTSMSTGLLYDLVIEQYKFFDLDVGIGRMILFNVMTFLWSWGLAGLCMPILIYPKQMMWPSVLGPCALFETLHVNTSISVPNWKVSRFRFLVYAFTGSFVLYWLSDLVIPFITYIGAFISWCKPSSAVLAQVFGTKSGLGLFPVTFSWPEITNLSNPLQTPFWAISNIYGSFFFWVLVILPACYYSNQWQTAHLPMLSNGIFNSSGKSYVASSVVNSSYRLDVEKYTKYSPVFLPIGFILKLCLGVGAFAAMMTQFFWRFKTDVIGAFKALKRNRIAVEPMPHMSKLHWGYYVFILMLALCLGFVFFYAWEDRYLSGGGYIVSCIIGVIIVVPTAMIEGKSSFTLAMDSFLDVVGSKWFAGDPIAVMFFYETSFGFIQHFMHMQQDLKLCTYTRTDLKRAMVVLLLASVWGSVMASAITYWYMFNIHGVCTTDAKDNLTCKSVKTQYNTHLIWGLFGEHIFGAGGRYTVVLWFFLVGAGVTGATIAMQKIRPXAKFWKQFSPALFMGGAQSIPTSTGINYHSWFIVGFIFNYYIHKYKTLWWRKYNLVLAVAMDCGVAIAAIIVYFAVVYPGGSANYNWWGTEVFAKGCDSTGCPYKSSASIQKPSGIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB11</accession>
    <name>A0A1E5RB11_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein-lysine N-methyltransferase EFM4</fullName>
            <ecNumber evidence="7">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Elongation factor methyltransferase 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">EFM4</name>
        <name evidence="9" type="ORF">AWRI3579_g2859</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that mono- and dimethylates elongation factor 1-alpha at 'Lys-316'. May play a role in intracellular transport.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class I-like SAM-binding methyltransferase superfamily. EFM4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RB11"/>
    <dbReference type="FunCoup" id="A0A1E5RB11">
        <property type="interactions" value="751"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB11"/>
    <dbReference type="InParanoid" id="A0A1E5RB11"/>
    <dbReference type="OrthoDB" id="10069295at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000287">
        <property type="entry name" value="Protein-lysine N-methyltransferase EFM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03188">
        <property type="entry name" value="Methyltr_EFM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026635">
        <property type="entry name" value="Efm4/METTL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12843:SF5">
        <property type="entry name" value="EEF1A LYSINE METHYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12843">
        <property type="entry name" value="PROTEIN-LYSINE N-METHYLTRANSFERASE METTL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 7 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Methyltransferase" evidence="8">
        <location>
            <begin position="68"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ84088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="236" mass="26906" checksum="91A0801EDBFF7F94" modified="2017-01-18" version="1">MEDTTKLNKSKLGTKQYWDDFYNKEKENFNENPDDTGECWFNDSDAEEKMIDFLMNNLNDTTSSVLINEKSTMIDLGTGNGHLLFQLWEEGFKQNKMLGVDYSEQSVLFSKDILEEVYKDAKDTIDFKHADIFNDSWNPGQFDIVLDKGTLDAIALSGLTFSNNQTVVDVYPNVIEKILKPNGCFLITSCNFTEQELIKTIEKSGHLKLWKTIKYPVFEFGGIKGSTICTCAFTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBA2</accession>
    <name>A0A1E5RBA2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AMMECR1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2777</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBA2">
        <property type="interactions" value="1089"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBA2"/>
    <dbReference type="InParanoid" id="A0A1E5RBA2"/>
    <dbReference type="OrthoDB" id="24630at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.700.20">
        <property type="entry name" value="Hypothetical protein ph0010, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023473">
        <property type="entry name" value="AMMECR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036071">
        <property type="entry name" value="AMMECR1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002733">
        <property type="entry name" value="AMMECR1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027485">
        <property type="entry name" value="AMMECR1_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00296">
        <property type="entry name" value="TIGR00296 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016:SF0">
        <property type="entry name" value="AMME SYNDROME CANDIDATE GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016">
        <property type="entry name" value="AMMECR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01871">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143447">
        <property type="entry name" value="AMMECR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51112">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMMECR1" evidence="2">
        <location>
            <begin position="5"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="276" mass="31804" checksum="14953867D6B8634F" modified="2017-01-18" version="1">MPSTATTTTXSSITSYSFYCFYTLKKALFSETLPRDELSYDKIKEKLFPTQDKDIPSSQKISLFVTWQKKRHSLLSGNVKSISNQNEEEKDDDDDDDDDESSYSLRGCIGTFGKIPLAQGIKQYALHSAFHDTRFNPIGKSDLPLLKVKCSILHTFETFYTHNPQLAKANAKKNLNDWIVGVHGIELVFKYHHSTYSATFLPEVMVEQEWDYEDTFENLIYKALEXKGTLNGSQAKYDVVAEISLNPQKYIVKVTRYQSSIYDCTYKQFLEKYKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RC97</accession>
    <name>A0A1E5RC97_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">COMPASS component SWD2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2473</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat SWD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RC97"/>
    <dbReference type="FunCoup" id="A0A1E5RC97">
        <property type="interactions" value="1059"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RC97"/>
    <dbReference type="InParanoid" id="A0A1E5RC97"/>
    <dbReference type="OrthoDB" id="27537at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016070">
        <property type="term" value="P:RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037867">
        <property type="entry name" value="Swd2/WDR82"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19861:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19861">
        <property type="entry name" value="WD40 REPEAT PROTEIN SWD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="108"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="257"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ84213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="326" mass="36389" checksum="08666A731FDC0115" modified="2017-01-18" version="1">MSFELSRESLRKFSPVKSFRLPKSETCPVTSLAFDDAGELLLATYANENIQLFDAVSCKFLTTIASKKYGCHASIFTHSQAECIYSSTSKSFDIRHLNLETNQYLRYFTGHGALVTDLVMSPTSDFFLSCSYDESVRLWDLRAPKAIAIVPAYTPNCVAYDPSGLVFALGNPSGNEIGLYNIKSLRDLPFLVIKLDRRFENQWNKLEFSNCGRYILVSGTSGKQLVLDAFDGTQLFTLQGVKPFPYREFIDSGSACFTPDGNSVLGTSYDGRIAVWDINNALSDRNLNPIVNISGVKDSSPRCIKFNPKHGMFVTADENLDFYVYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDM6</accession>
    <name>A0A1E5RDM6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">tRNA pseudouridine synthase 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">tRNA pseudouridylate synthase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">tRNA-uridine isomerase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AWRI3579_g1890</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Formation of pseudouridine at positions 27 and 28 in the anticodon stem and loop of transfer RNAs; at positions 34 and 36 of intron-containing precursor tRNA(Ile) and at position 35 in the intron-containing tRNA(Tyr). Catalyzes pseudouridylation at position 44 in U2 snRNA. Also catalyzes pseudouridylation of mRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a uridine in mRNA = a pseudouridine in mRNA</text>
            <dbReference type="Rhea" id="RHEA:56644"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:14659"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a uridine in tRNA = a pseudouridine in tRNA</text>
            <dbReference type="Rhea" id="RHEA:54572"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13934"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>uridine in snRNA = pseudouridine in snRNA</text>
            <dbReference type="Rhea" id="RHEA:51124"/>
            <dbReference type="Rhea" id="RHEA-COMP:12891"/>
            <dbReference type="Rhea" id="RHEA-COMP:12892"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDM6"/>
    <dbReference type="FunCoup" id="A0A1E5RDM6">
        <property type="interactions" value="1027"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDM6"/>
    <dbReference type="InParanoid" id="A0A1E5RDM6"/>
    <dbReference type="OrthoDB" id="10256309at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02568">
        <property type="entry name" value="PseudoU_synth_PUS1_PUS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.580:FF:000002">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.660:FF:000002">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041708">
        <property type="entry name" value="PUS1/PUS2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00071">
        <property type="entry name" value="hisT_truA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF4">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase I TruA alpha/beta" evidence="18">
        <location>
            <begin position="333"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="14"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="60"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="15">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00079072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00080858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR641708-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR641708-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OEJ85005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="553" mass="62883" checksum="8ECE7C84641FE008" modified="2017-01-18" version="1">MSLFTKIGQLAKQLFGKPAPAAETSAKSIGNKRSSSEANLGDANDKSNKKFNNKFNRNNKNNKAKPKKDKYKGKKTPFQGQARVYTPKEGVDPNAPKTPRLPKRKVAVLFGYCGAGYHGLQYNPPSHTIEAELYNAMVKAGAVSEDNAVDPKKSSLQRAARTDKGVHAGGNVISMKLIIEDPDIKDKINACLPEGVRVWNIQRVCNGFDSKNACSSRIYEYLLPTYALLGPNPDTQLYSDLHDKSKNGDLDYKDEESDEFFAKYQQMISENFTAEELQKIKNFEFIKPDDFDQQGSDVREVYQLVKKHKSLENSFRRDYRVSTKRLNYLRQVMSQYLGAHNFHNFTLGKDFKDPSAIRYMKNIKVSDPFVINNTEWVSIKIHGQSFMLHQIRKMISMATFIVRCGASKTLIHEAFKADKLNIPKAPALGLLLENPVYSTFNERLNSLGYESIDFTHFEKQMTDFKMRHIYDKIYAEEVKENVFYAFFGFVDALNKVVDSKSHENSDKTSCFQYFVTAKKRSEDAEDDVIYTDVKQLKKEDAATGAKTSRKSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDN7</accession>
    <name>A0A1E5RDN7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Succinate dehydrogenase [ubiquinone] cytochrome b small subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g2085</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the CybS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDN7">
        <property type="interactions" value="84"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDN7"/>
    <dbReference type="InParanoid" id="A0A1E5RDN7"/>
    <dbReference type="OrthoDB" id="18577at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048039">
        <property type="term" value="F:ubiquinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03496">
        <property type="entry name" value="SQR_TypeC_CybS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase, transmembrane subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007992">
        <property type="entry name" value="CybS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13337">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13337:SF2">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] CYTOCHROME B SMALL SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05328">
        <property type="entry name" value="CybS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="Fumarate reductase respiratory complex transmembrane subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <note>ligand shared with SDHC</note>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>a ubiquinone</name>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <note>ligand shared with IP/SDHB</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR607992-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR607992-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ84996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="199" mass="22554" checksum="C2D8231F120D6370" modified="2017-01-18" version="1">MLIRPVGLQNRMMWGLNTQVKKCTTRTFTPFAAFSFQQLSQQRHLSLKPNFQKLKLIPQAPGGVQGQLNEPLKKAETNYFEGSFHWDYERIVAISLLPSVMFPMYCALSSGTAIYPVADAILGSLLLIHCNYGFQSCIIDYIPKRKFGFWHSFAKGMLRLGXVMGLYGIYEIETNNNGLCDLVGKLWKDPESGLYLFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDX8</accession>
    <name>A0A1E5RDX8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Replication factor C subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2146</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDX8">
        <property type="interactions" value="852"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDX8"/>
    <dbReference type="InParanoid" id="A0A1E5RDX8"/>
    <dbReference type="OrthoDB" id="761538at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031391">
        <property type="term" value="C:Elg1 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031389">
        <property type="term" value="C:Rad17 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000002">
        <property type="entry name" value="Replication factor C subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000136">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF1">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13177">
        <property type="entry name" value="DNA_pol3_delta2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22534">
        <property type="entry name" value="RFC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="36"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00070185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="369" mass="41455" checksum="8BD51A19ED927AEB" modified="2017-01-18" version="1">MSLWVDKYRPTNLSNLSHNESLKHQLEQLAQNNPHDLPHLLFYGPNGAGKKTRAMCLLNSIFDSSIYKLKIDVRQFQVNSRKLELNVVSSPHHIEITPSDLGGSXDRVVIQELLKEVGQTEQVEFGQAANSVAKDGISHQSPKFKVVIINDANSLSRDAQSALRRTMEKYSKNLRLIMLCDSLSSIISPIKSRVLLIRTPTPTMIENMGILQNVCVKEKVSVESKDILEKIVDYSNGNLRVSLLCLETMALSNDLRLKDNTALVKPDWKVVISKMGTRIIKERSVSSLIECRSTLYDLLAHCIPADTIMHELTLDLLEKLSENSNDTLKLKVVSWASVFDERLALGSKAIYHLEGFIARVMXELDASRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDZ4</accession>
    <name>A0A1E5RDZ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2358</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RDZ4"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ85115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="237" mass="27729" checksum="7563125FEA596A62" modified="2017-01-18" version="1">MVHNVFPLTDDENLQKSSIVTYTVIDNRHLVYVDDSNLLHFLNIEQSTQNIGKSKLIKTVNLGSYNTRQIKKLIYHEYENNGMYLIWSDDXSISWAKLDRLLLXSAVSDDLQNCSQKQLSYKWKLNDPYARIMDFQACNKSHMMLFFVVTNTGSIINFRFYKELSKRSLQILEHRVTKKPRPKNSSXNAKYVFKQDNLVSHXKKIAHDSFSELKLFSSTRXSMEFPNYQSLQAEKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE47</accession>
    <name>A0A1E5RE47_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE47">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE47"/>
    <dbReference type="InParanoid" id="A0A1E5RE47"/>
    <dbReference type="OrthoDB" id="5304883at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="511"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="276"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="347"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="392"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="426"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="437"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="480"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="529"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="808" mass="90818" checksum="7EC903B29634652C" modified="2017-01-18" version="1">MKSQEGETQMTLPPTISRLLDNNENSSRNSFFSDVDLDERTGRYEHYLKIGFSGQPDLANIPLSERSLERKSDFELDNDDLDLSSDKNYLACRNDFPGQFDVDALSVXNENAMEENEDRGNVHDEREEAEREDSYGFQDVYMEKLPLKKSNKQAYNFANNFVEAARTSQDTLLLSPVPRTASPLRSQRFFNESSNFELPTRSHPHSGSKNVHFMEHDFADNEYGLDSRPVSAVSIESSLFASPQQQRRGDYSFNYKLNQSVLDMKNNNNCGEKMLNNSINSTSNNYNTSTSHRGTRASGSSEQNELSPVGSHAGRHRKPHIEELISNAQNLSLYLDQAIDLTTSTANDFTRNSTRGHTMDGNSERETSMCSGSGNFGEASHSEMESFCMEPSNSSIVSNQYSSSNRHPDSINHNDHHHHHHHHKSQEHPQKKDQKSFGESSEISSFALSPMSNXEERTMIGTRNGGTISTSPLSAAVSGSRPNTSTPHNVTDTGPHKIDDFLISYPLNNNLSLQNDEQSDDTSSRKNEQEDEDLDVEETSFLTKPWSAEEFDTSIMSNYETQDAIELFINMLNYLLTLTKLENQSQTFFEQRSFXKDTFDSFLMKGPASLSYEKFVKNRIQTRCKYSSMIYLNSAYLLLNLLTNYSCHSLPPKSVEMEDGFSENTNTDKANHKRKPAKLQLALPLTESHVHKLIVATLRISSKLIEDNIHSTSFFSKIAGVSKKSLVLLERDFLKLLLSTISTNTKELVXSASMSSIATATTIQVAPSTPTPIPSPLAMSVSPPVLGNDFNWLLINDMKLIEMVNLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE54</accession>
    <name>A0A1E5RE54_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Crossover junction endonuclease EME1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RE54"/>
    <dbReference type="FunCoup" id="A0A1E5RE54">
        <property type="interactions" value="199"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE54"/>
    <dbReference type="InParanoid" id="A0A1E5RE54"/>
    <dbReference type="OrthoDB" id="343092at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="673" mass="76475" checksum="E9B319380902561B" modified="2017-01-18" version="1">MSQIEIIDLDDESEISHHALSDNASKCTSPNVICENSSITISDNESSSEEVEVIESAPYEINAKTSKGGADLSEEISFAFQNDTTLSNHANATRSFVAGSSQNANKKIKISSTQSLVQEIIDDSSDEDLSGALIEKFRQKNARLTGKHRLSSSINSSRIIGDIDFSFERPVDFGNSEVNVLSQGSQKELNRVPSSPKDREPVADLFLNSSPIPKCLFDIEKSPINVEKESDSNKIFYSSASSSKNTKEAAGQSSDRTHIMVDKQPFTKSLQKTHLVRKKTDIPILDLANNERHIQTLSRSFTSPSVELNPQRTREYDNSNEPSHNGKKTNPLRTFNYITNAKNFSEQEFQGMLHYFNRSSALKKSLTEVNKVHRTTEELCLEIVMDFHPGVYNMIKDEGIVLEEELMCNVSKSNSFIASSICFKRKVRSIYDIDHDIFFPCSEKLVEEPSIMMFYDSFQFFLNLQENKSELLYTLQKLKDKYMRVVVVLNNFQKLESSLRRLYNGIYISEVRNNLHPGEQRKKTSKKSEELEKLKLRAEDLHVKITEVAVLCGVHVFPMANTANFVEWSKNILLNVSRSRYETLLRNIECSHLHVKSGQTPLEVFEKSLEQFNMITKAKSQRISSEYPSFQAFFQNMNNIRKGPDGKNLIPPSSLEAIQILFSADDPFQFIYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REA2</accession>
    <name>A0A1E5REA2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Non-SCF-type F-box protein ROY1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1696</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REA2">
        <property type="interactions" value="124"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REA2"/>
    <dbReference type="InParanoid" id="A0A1E5REA2"/>
    <dbReference type="OrthoDB" id="3976101at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="16"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="543" mass="62677" checksum="02BE0D3B68F436BE" modified="2017-01-18" version="1">MPTITLPANNFLKSFEIWPNVIDFLDQTSLCNLAQCSKNLREISETKLYANIHIXSNPILKXEQWFIDTNETYVFGYRSMIKSKDQNDLFLYDKLERLIESRKLFKVKKIVICAQPFFDAXSGNAVLKRLLNELISLGKVHTLDIQDTYLFNAYYNSLLKISQNLQTLRLKSLTDLDHVHSLCTTLQIDSASGSGSCTVPQSLDGIKTLVLSNFEALSILEFLQTREVMLHGVESLALYHQHDSNTKPWNIEFLLKVVPVEKLKVLEIGFSCEVEECECSQTFFSTLGPLLFNLSELSLIEKSIETPTHHNSKEKWDIDVNHFIIELPEFGAHLKKLSIMHNIPANGQIADSVDGNYFRRRKMYEQMLPHLINLEVLIAPTFIQSLSCFEVLSCDFLWNGCRCPFCQKVLAVMDKYIHNHVFFNDWFADFKDIGPINLVAYASTVLSNRLISKDHNQLKVFGEAPLDVYWDFHGFDSIGHYRGYTCYFDESAFKPLAVCFQHFTDTYMEYIVHFLPELRFCCLSGIYYYIEDHAFHSVYDNAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REA4</accession>
    <name>A0A1E5REA4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1922</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REA4"/>
    <dbReference type="InParanoid" id="A0A1E5REA4"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF236">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="339" mass="37831" checksum="8240F2414087A3E2" modified="2017-01-18" version="1">MDASKFPTAPKYSRIDFLCQNYQGFRPYKAVFLPKDYPDLTGKXAVITGMNTGIGYHVTKLLLAQNCEVYGLVRTASKGEEAKARILKELGTEEKGSLTIVGGCELSDFAKVKEVGLKLQNELLKDKTINIIIHNAGLMSALNNTSNEKEGIELMFATDVMGPQLLQNYLDPLFLKKDSDLKRIVWVSSLAHQNAPWPYGINFEDPFYKNLSNRPSSYTLYGQSKACNILQAKAWATQNNASKYGIISTSCFPGIITTDLMRDYPSGMQKISKMFFSAPVYGAYSELFAALSPTIKRQAEYVLPFGKIGKPRADIAAAMENGIDVQFWEFIQESLKEYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REG3</accession>
    <name>A0A1E5REG3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Bud site selection protein RAX1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2102</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REG3">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REG3"/>
    <dbReference type="InParanoid" id="A0A1E5REG3"/>
    <dbReference type="OrthoDB" id="5584247at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052246">
        <property type="entry name" value="Cell_Polariz_PKAAnc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13155:SF1">
        <property type="entry name" value="A-KINASE ANCHOR PROTEIN 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13155">
        <property type="entry name" value="A-KINASE ANCHOR PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="448"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="483"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="643"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="3">
        <location>
            <begin position="331"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="522"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="169"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="199"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="522"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="533"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="545"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="666" mass="76178" checksum="C46BE8B059F6FD47" modified="2017-01-18" version="1">METHSRKYHNSNATTSPPISVVKHTPNRFSVIMFGRNNSNNNGRIVSERKDLLNYEDGDFDTSNQLSTNRNLDGDYYLDMDTHILDRLPNLQEVLNKQTVTPVDLWSFYTFISSYPQALNCLDFYIDIATHLRFCKEYVRDVRKSVRSFVDKEQHLLRNGKTTSRNLNSHSGSQHYHSGSSRSSEPTVASAFLYSSQSPQPESLXQQQNQQSLNNGLNDFMGSLDDGINNANLLGKQDITQMHRSSKRVSQFLNKDLQSQLMNNVLKTWRDSSLSVTREDFQNSSDEYDILSEIKNDPPQNDTRVQEPLKDLQFEWRRSREIDAFISGQGIKTKLNSQKLLKNAESIVSTYLLSPTKSPKYLTHMPESLKDDIIHQIFNCKKYDPEIFETCKELSFQILEQDMFPIFLQEVGFHNLHDRSYNYSQQLSSGTQVSTSHLVPSIGRFPQVYRISIGLVFLWIALWVGYTLIFLNYKRQIRVVTLVPFLFGWYFIITGITRVDLLYAWAGVTQSLHNVAPDMFGSSESVNTSNSKINDKEQQTRKNENLGNIPQGTNVSGSFASVSSSDKNSAHSFINHTMAHKSSWNLLELGVFSSSSKTSYHEVPWTIRLMQCDFLKKGNHRGNIVVIKSPFITRLIXKRSFSVLAWIVLMTAISTVVFSCVPGQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REP7</accession>
    <name>A0A1E5REP7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein ORM1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2344</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REP7">
        <property type="interactions" value="541"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REP7"/>
    <dbReference type="InParanoid" id="A0A1E5REP7"/>
    <dbReference type="OrthoDB" id="1932233at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007203">
        <property type="entry name" value="ORMDL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12665">
        <property type="entry name" value="ORMDL PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04061">
        <property type="entry name" value="ORMDL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018147">
        <property type="entry name" value="ORMDL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ85033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="227" mass="26360" checksum="E0310FA23B494238" modified="2017-01-18" version="1">MMNDITMNITEIEEIPFPSSEKTETNNTNAFHNLSTNDIQIEPVKDHRRRRTSSIISHVEPEXIEDENDQTMSMNMNVLWINQRGAWLIHFLIVIFLKIFFTLVPFTQHTNQNELSWTLTNSSYCIGSYIMFHLIKGTPFDLNGGCFDNLTMWEQIDNGLQFTPARKFLIGFPICLFLVATHYSRFNLQYFVFNLLMTICIAVVPKLPWTHRLRITIPGITSPAQIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RER7</accession>
    <name>A0A1E5RER7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Small glutamine-rich tetratricopeptide repeat-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1813</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SGT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RER7">
        <property type="interactions" value="607"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RER7"/>
    <dbReference type="InParanoid" id="A0A1E5RER7"/>
    <dbReference type="OrthoDB" id="2335338at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072380">
        <property type="term" value="C:TRC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006620">
        <property type="term" value="P:post-translational protein targeting to endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000207">
        <property type="entry name" value="Small glutamine-rich tetratricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047150">
        <property type="entry name" value="SGT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032374">
        <property type="entry name" value="SGTA_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45831">
        <property type="entry name" value="LD24721P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45831:SF2">
        <property type="entry name" value="LD24721P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16546">
        <property type="entry name" value="SGTA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 4" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="SGTA homodimerisation" evidence="6">
        <location>
            <begin position="39"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="198"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="248"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="360" mass="38487" checksum="87D66282F7D4D769" modified="2017-01-18" version="1">MGKNTLNNTSFNKILILLLANLLKKEKRNTNTQHHTMSSNKTISALIIDFLNTVIEKKEVQEEFTDSLNVAIDCIAEAFEIEREDVKTLTGGKTLAQLASGATVATASSAASSSKVEVKVEDDPKAKAEAETLKLEGNKAMASRDFALAISKYTAAIEAYPSNPIYYANRAAASSSLKDYESAVADAELAIKIDPDYSKGYSRLGFAKYALDEPQEALEAYAKVLEIEGDXATAAMKKDYETAKAKVESSLNLEKTEKEASSENTSGGNPFGGAGGMPDMSSFMNGSGLGGLGGMLNNPQVMEAAQNMMKNPGMMQEMMSNPAIKQMAEKFSGGNGMPDLGSLMNDPSIKDMASKMFGPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RES4</accession>
    <name>A0A1E5RES4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RES4"/>
    <dbReference type="InParanoid" id="A0A1E5RES4"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020401">
        <property type="entry name" value="mRNA_transport_factor_GFD1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17331">
        <property type="entry name" value="GFD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="228" mass="25681" checksum="EBF615B56FC2EE13" modified="2017-01-18" version="1">MWASSSEDEDTNEKAQPFAQKQSQNNQKKNSFSNRKNSGSWQNSSTQRNNLKSTNHRSESFSNQKKKNNKNSGNFNNEKIKQNPLAAALGLNLAELQAKREGSSDSSEYETTDDERDTFDSRNGTSHKTLAERLGKPINANNRSKTGTTKDENIKIKNKKTPVSNGSATSVESTSQLSKVDLLKKKIAEQKKILEKTHEKKKIMDSIDMLTKDLDWDAEEIDISKIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RES5</accession>
    <name>A0A1E5RES5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Trehalose-6-phosphate synthase</fullName>
            <ecNumber evidence="6">2.4.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">UDP-glucose-glucosephosphate glucosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2449</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>D-glucose 6-phosphate + UDP-alpha-D-glucose = alpha,alpha-trehalose 6-phosphate + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58429"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="2.4.1.15"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.15" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RES5"/>
    <dbReference type="FunCoup" id="A0A1E5RES5">
        <property type="interactions" value="618"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RES5"/>
    <dbReference type="InParanoid" id="A0A1E5RES5"/>
    <dbReference type="OrthoDB" id="755951at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005946">
        <property type="term" value="C:alpha,alpha-trehalose-phosphate synthase complex (UDP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000007">
        <property type="entry name" value="Trehalose-6-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000035">
        <property type="entry name" value="Trehalose-6-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012766">
        <property type="entry name" value="Trehalose_OtsA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02400">
        <property type="entry name" value="trehalose_OtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF106">
        <property type="entry name" value="BCDNA.GH08860"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="484" mass="55333" checksum="A3414C39365740C8" modified="2017-01-18" version="1">MSENKHPGNVIVVSNRLPVTISKNPESGEYEYKMSSGGLVTALQGLAKHTTFQWYGWPGLTLPEEDKPVVCKDLKEKFNAVPIFLSDEVADLHYNGFSNSILWPLFHYHPGEINFDENAWLAYNEANLKFMDSIIENLQENDLIWVHDYHLMLLPQLIRNRLKKTKPELVSKIKLGFFLHTPFPSSEIYRILPVRQEILKGVLSCDLIGFHTYDYARHFLSSVQKVLNINTLPNGLEYDGRFVNVGAFPIGIDVDSFTEGLKQDDVQKRITELQNTFKNCKIIVGVDRLDYIKGVPQKLHAMEVFLQQHPEWIGKVVLVQLAVPSRGDVEEYQFLRSVVNELVGRINGAFGTIEFVPIHFMHKSINFQELISLYAVSDVCLVSSTRDGMNLVSYEYIACQEKKKGSLILSEFTGAAQSLNGSLIVNPWNTDELAESINEALTLPEEKKEANWEKLYKYISKYTSEYWGENFVKELNNLENTIQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REW6</accession>
    <name>A0A1E5REW6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Vesicular-fusion protein SEC18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g2122</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 12">Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REW6"/>
    <dbReference type="FunCoup" id="A0A1E5REW6">
        <property type="interactions" value="844"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REW6"/>
    <dbReference type="InParanoid" id="A0A1E5REW6"/>
    <dbReference type="OrthoDB" id="9982946at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035494">
        <property type="term" value="P:SNARE complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19504">
        <property type="entry name" value="RecA-like_NSF-SEC18_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000026">
        <property type="entry name" value="vesicle-fusing ATPase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000166">
        <property type="entry name" value="vesicle-fusing ATPase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000012">
        <property type="entry name" value="Vesicle-fusing ATPase protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000187">
        <property type="entry name" value="Vesicular-fusion ATPase SEC18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039812">
        <property type="entry name" value="Vesicle-fus_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23078:SF3">
        <property type="entry name" value="VESICLE-FUSING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23078">
        <property type="entry name" value="VESICULAR-FUSION PROTEIN NSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 12" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="15">
        <location>
            <begin position="36"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48" evidence="14">
        <location>
            <begin position="141"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="284"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="567"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ85424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="775" mass="86218" checksum="0BFC336D60CA53F2" modified="2017-01-18" version="1">MEKFGGLANKLSSFGKITHNVPPPMDVSNLDQAPRMLVVENCPDKNYALSNVAAVNPTDFPDNVYVIIDNLFVFTTKHAPINPGCIGLNGNQRTWGGWSLKQPIESKVFDLFRYTHGKPCYLGVLDIEISFRSRAKAVATPFDQDELAKHFTKIYESQIFSPTQMLVMEFQGLHFDLRIRSMQNIELGDIDMSTSTPLSNGIEVKGILTKQTQINFYKGRDGLVNLKSSGNSLRPRSDAVIRPDFKFEDMGVGGLDKEFTQIFRRAFASRIFPPDVIAKLGISHVKGLLLFGPPGTGKTLIARKIGTMLNAKEPKIVNGPEILSKYVGSSEENIRNLFKDAEAEYKAKGEESSLHIIIFDELDSVFKQRGSRGDGTGVGDNVVNQLLAKMDGVDQLNNILVIGMTNRKDLIDNALLRPGRFEVQVEIKLPDEQGRLQIFEIQTKKMKDNNMLDHDVDLKELASLTKNFSGAEIEGLVKSASSFAINKTINIGKGVTKLNNKDITNLKVNRQDFLNSLSEVTPAFGVSEEDLKTCVENGIIKYSPKVDDLLKNGARYVRQVSNSDKSRLISLLFHGPPGSGKTALSASIALQSGFPFVRMISPEEIAGMSEQNKINYIDNTFRDAYKSQLNILVIDNIETLIDWVPIGPRFSNNILQVLKVYLKRKPPKDRKLLIISTTSTYSVLKQMDILSCFDNEIAVPNVQNMEDFFNVLVSTKFGDSEEDRVAVINQLTKVVPEGKLNVGIKKVLTNIETARHDEDPVNELIDLMAQSSSAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF10</accession>
    <name>A0A1E5RF10_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small COPII coat GTPase SAR1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Small COPII coat GTPase sar1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">AWRI3579_g1881</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">COPII-coated vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 21">Belongs to the small GTPase superfamily. SAR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF10"/>
    <dbReference type="FunCoup" id="A0A1E5RF10">
        <property type="interactions" value="937"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF10"/>
    <dbReference type="InParanoid" id="A0A1E5RF10"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00879">
        <property type="entry name" value="Sar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000161">
        <property type="entry name" value="Small COPII coat GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006687">
        <property type="entry name" value="Small_GTPase_SAR1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45684">
        <property type="entry name" value="RE74312P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51422">
        <property type="entry name" value="SAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10 21" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 21" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="13 21" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14 19" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 21" id="KW-0653">Protein transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 21" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="28"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="130"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR606687-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR606687-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU003926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="OEJ85153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="188" mass="21237" checksum="31BB115E05F72A42" modified="2017-01-18" version="1">MWGVVNWFRDVLASLGLWNKHGKLLFLGLDNAGKTTLLHMLKNDRLATLQPTWHPTSEELSIGNIKFTTFDLGGHIQARRLWKDYFPEVNGIVFLVDAADPQRFDEARVELEGLFQIPELKTVPFVVLGNKIDSPNAVPETELRSALGLMSTTGSSRVEGQRPIEVFMCSVVMKNGYTEAFEWLSQFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFE0</accession>
    <name>A0A1E5RFE0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1773</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFE0">
        <property type="interactions" value="93"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFE0"/>
    <dbReference type="InParanoid" id="A0A1E5RFE0"/>
    <dbReference type="OrthoDB" id="5579088at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010945">
        <property type="term" value="F:coenzyme A diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034389">
        <property type="term" value="P:lipid droplet organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019388">
        <property type="entry name" value="FIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23129">
        <property type="entry name" value="ACYL-COENZYME A DIPHOSPHATASE FITM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23129:SF0">
        <property type="entry name" value="ACYL-COENZYME A DIPHOSPHATASE FITM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10261">
        <property type="entry name" value="FIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="9">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="379" mass="44734" checksum="2FE342A04AACBB6F" modified="2017-01-18" version="1">MILSHFIQLWLPTSPFLKYLFTNINKKNFINLIFVKKGWFWSSCIGWWNIFIREPLPNEXKNIDETSLXDQNXESKYHKNKXPNQKQPIGIFKKITNTPLKRFALLIAAWYICTQSLILHRGSLIDLIFVSTGGTCQPPAFVPDFISSSNSNSNSNSNSNSNSDLNLEDLHLSNENNGWETELNKWVKRGFKHLKKQTGKSSTWEILQYIIDNKASFFTKEFGLLIKKEHAKFNSIQCKKIGGQWIGGYDISGHVFLLNLMIWWLLGEHFLYCKAQRQQERNTKKLKSKNSFTIQYDNLKTLARNTYHCTEWTERKQLFKNLLIKTLHFVTIDQSWIMVYFLVFLWWYNFLVTCVGFHTFGEKYWALFVSYIVTYKIYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RG55</accession>
    <name>A0A1E5RG55_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GTPase-activating protein GYP5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1757</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the GYP5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RG55">
        <property type="interactions" value="173"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RG55"/>
    <dbReference type="InParanoid" id="A0A1E5RG55"/>
    <dbReference type="OrthoDB" id="295078at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000044">
        <property type="entry name" value="GTPase-activating protein GYP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23436">
        <property type="entry name" value="RabGap-TBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="12">
        <location>
            <begin position="470"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="36"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="128"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="392"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="844"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="907"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="10"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="198"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="214"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="259"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="907"/>
            <end position="916"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00072088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ85553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="941" mass="106806" checksum="CB8EE0F63130D26E" modified="2017-01-18" version="1">MNTDEKKLQSAETNTANDSIDTNTALEEVDELANVAATSGAPSDTKSNEIFLEAETHEASLDKAEPPKLANQNENDAQEQFATADVSNNENGEEPSSKDPEFTEAVSEKQTVVNNAEESLKAFMVDSPVDATHDSNAMNHKPEEQHDQEFVSQLKQLKLAKPVETSQLSNIDDTTSRDADIETISTDQDKTLDEDSVIVNSSMIEQSTAQKSVVPPPVPPPVPPRDVTAEAKVPPSLPPRENNSKATNQGPNLLPALQFSRQRTRNASPASSRTSTHEHQLSPQQNVKFKKSMLQIHAVPPPLSEEYKSPRFLRNLKINDAGSSQELNARQGTSLTDHVEQFRINSTATSSDYDMIVDRLNENSKSDITNQESVLESNTALKYTFKGIKDRLTSKSRRHRRSRQELSETESPSINGVGDTVEDEHSIISYAEDEAKEIAIDWPFWTSVVYDYEKVAQQEPEKMELEISKGIPSQIRGVIWQLISRSKSKALELLYANSINQECVHENVIKRDLKRTNFIPHDRLDNLFNVLKAYANYDTDVGYTQGMGFITTPLLLQTDSESDAFGLLVRLMESYKVREMFLPDMPGLMLKLYQFDRILEESSPGLYNHLARQGVRSTMYATQWFLTFFGYKFPLEFVLRIFDIVFAEGAEALLKFAIALMLKNESNLLALKFDQLLEYLKNDLFNSYLHXSLENGGKRLGSSNYSKKSRSALSLFRTNSEVSYSSLSNQDDKKAEEDTAADSCLEKYNVDEFIQDAIKIKIMPISLKRYEKEYAEIHEIEKQKEQQFESLRIQNKNLEKELEKLKHDFNSLKHDHEAIANELINKRLYIETVADEKRDLEEQLAQERRKNSLPNPDSELPSDLQHDLKVTMERNLEVMSKNENLEMEVASLNKVIAQLNEEKRHAWGIDNNHSESTDTSTDSSKLFRKKSLPGPSWKIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGR5</accession>
    <name>A0A1E5RGR5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein HGH1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1145</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HGH1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGR5"/>
    <dbReference type="FunCoup" id="A0A1E5RGR5">
        <property type="interactions" value="1104"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGR5"/>
    <dbReference type="InParanoid" id="A0A1E5RGR5"/>
    <dbReference type="OrthoDB" id="338814at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039717">
        <property type="entry name" value="Hgh1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007206">
        <property type="entry name" value="Protein_HGH1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007205">
        <property type="entry name" value="Protein_HGH1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13387">
        <property type="entry name" value="PROTEIN HGH1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13387:SF9">
        <property type="entry name" value="PROTEIN HGH1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04063">
        <property type="entry name" value="DUF383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04064">
        <property type="entry name" value="DUF384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein HGH1 N-terminal" evidence="4">
        <location>
            <begin position="93"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Protein HGH1 C-terminal" evidence="5">
        <location>
            <begin position="292"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="342"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="373"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ86085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="390" mass="45102" checksum="DB8B28BCF83853DB" modified="2017-01-18" version="1">MPTQIEELVSFLHSDQPAVRQIALENLVGFSTNPSIFKYDNYRSVTDLKKLSQENSQILVRQSVTILANLCDDPVFRKLIVQDEDYMSYLVWKICDLQNTSADIMSILLSNLSKDDSIIKVFDIVKVNSLVKIDKQVFKSERAIDCLMDCFVKGYDRKLNKFANFDYLAYFFADISRFRKGREYFITKQDYDHVIPLQKLLVFTEKYDEAKTRREGVAYTIKNSLFDSEKHESLLTNEELNLLPYILLPIASAKDSEIDDEDMFNLPEELQLLPEDKQRDPSNEIICVYLESILLLCTTKAAREYMRSKSVYPLIREMHKNVENDDIDELTVRVVNMLMRGDPNTPVEEMPVKSAEDLPLEEDDAIDANTEAEKEDESDDEDDEAIVEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGT1</accession>
    <name>A0A1E5RGT1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Maintenance of telomere capping protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1095</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May be involved in telomere capping.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the MTC6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGT1"/>
    <dbReference type="FunCoup" id="A0A1E5RGT1">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGT1"/>
    <dbReference type="InParanoid" id="A0A1E5RGT1"/>
    <dbReference type="OrthoDB" id="5573651at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051008">
        <property type="entry name" value="Telomere_Capping_Maintenance"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057530">
        <property type="entry name" value="TIM-barrel_MTC6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35518:SF2">
        <property type="entry name" value="MAINTENANCE OF TELOMERE CAPPING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35518">
        <property type="entry name" value="MAINTENANCE OF TELOMOERE CAPPING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25506">
        <property type="entry name" value="TIM-barrel_MTC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184740" description="Maintenance of telomere capping protein 6" evidence="11">
        <location>
            <begin position="27"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="473"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="MTC6 partial TIM-barrel" evidence="12">
        <location>
            <begin position="38"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ86066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="523" mass="59557" checksum="0F2D1238600979A4" modified="2017-01-18" version="1">MKVHIHHLQALFVSFIFIFPLSFANALHLEMTSPQIYPNLSTQAFRGLIIQRDILKSVRLSQLPSLGVNFTQVLLPSNSFHFGNSTEMENYILVLLNSGIGTLGVDIFFESNRWKIYNTDLSFAWFLSIIQKYIGNTQSEVSVTSLFLLLKVRYLGTATEEMSFNTTTMNGLNHDIEANINNNKILSPQQQDTNQTWPTLNNFLYSSSQRLVIFNAGDDTYTNTVFRGNNTFVFTNEAMNYLHIPDQSEISIEFPVPSNLNFPVVASDNYNETSIRDVLYNGYTPLISNPDLIQIPYFLNSSLIWGWKKNQPSETYALDPFASDTNKISSFACASLQYDYTTNSSYWEVNDCYQKLPILCHNAVSNEWYIPTMQKAQFFSITSDNDQVSSNYGCPEGYLFNLPTTPLAISELNYAFSKQYEEDQKNFYFKKGTNNSIWIDLNSVSASNCWVVGGSHAICPYAKYVTSRNFVKMVLPLACCIGALLLMVIMLKFRKLPIQNNRRRWKRIVKESEKNAAADGVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGT5</accession>
    <name>A0A1E5RGT5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S20</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1316</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGT5"/>
    <dbReference type="FunCoup" id="A0A1E5RGT5">
        <property type="interactions" value="846"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGT5"/>
    <dbReference type="InParanoid" id="A0A1E5RGT5"/>
    <dbReference type="OrthoDB" id="10248551at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.600:FF:000004">
        <property type="entry name" value="30S ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="entry name" value="Ribosomal protein S10 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00508">
        <property type="entry name" value="Ribosomal_uS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_uS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_uS10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_uS10_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005729">
        <property type="entry name" value="Ribosomal_uS10_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01046">
        <property type="entry name" value="uS10_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S10 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00971">
        <property type="entry name" value="RIBOSOMALS10"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="Ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS10" evidence="6">
        <location>
            <begin position="20"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="117" mass="13235" checksum="99876A5545548922" modified="2017-01-18" version="1">MADVEKKEIEQPQETFLKIRMTLTSTKVKELEQVCSSIIDNAVNNQLVKKGPVRLPTKVLKITTRKTPNGEGSKTWETYEMRIHKRYIDVVSPASLVKKITQLTVQPGVEIEVTIVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGU8</accession>
    <name>A0A1E5RGU8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">threonine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Threonyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g1226</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGU8">
        <property type="interactions" value="86"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGU8"/>
    <dbReference type="InParanoid" id="A0A1E5RGU8"/>
    <dbReference type="OrthoDB" id="5423599at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070159">
        <property type="term" value="P:mitochondrial threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000039">
        <property type="entry name" value="Threonyl-tRNA synthetase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF50">
        <property type="entry name" value="THREONINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="13">
        <location>
            <begin position="43"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ86130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="464" mass="53786" checksum="09290A1636A1FEB6" modified="2017-01-18" version="1">MRASLKKLKVLRFCRLNSTKTESATNTSREVAHTQQLFFTDPVSPGSVFFLPNGAKIFNKLVNFMKLQQQHKHGFQEVITPLIYKKSLWETSGHWVNYKDDMFKVEGQDATKEEYGLKPMNCPGHCCMFEKFDRSYHELPIRYSDFSPLHRNEASGALSGLTRVRKFHQDDGHIFCTPEQVEEEITKCLKLIEMCYTRVFQLAPVEKQIQPXNTNLDGNSNKSSPYSLTLSTRPEDKYIGNLDVWNMAESTLKTILEKTGKPFTINEGDGAFYGPKIDXRVTDHVGKQHQVATIQLDFQLPERFDLKYKDKDNTYKRPIMIHRAVFGSIERFMSMLIDSNQGKWPFWLNPQQAVIIPLNTKNEKIMETSQKLWEKLRNTSEVDDMEPVKLNSFHFSADIDDRAEPAGYRIKDAIMKNYSYLIIVGEKEIENGKFAIRTSTSRKLEHLSMDEIFNKFVSLEESYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGW5</accession>
    <name>A0A1E5RGW5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1132</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RGW5"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ86141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="216" mass="24761" checksum="4F647D5564D09AB7" modified="2017-01-18" version="1">MVDFLILYASTSVYLKIKEHKIWQKLWRRKKRAXIKEDTVQDFPAQVEVPARKLLDSSXVFVSNSRKNIFMSLSETSSKIVSTWSLQLNHLSTSPITASTXSSITIGTTDLPNKITPNVDIFLSSFQWCAFKYGFNKIMFLPNLHAAQKLWGLVLLLASINQIKPLNVPTGFTKSVLKNTIKSLTTHHLFYDNTNKRARNLQKLKLLIMIFSNYFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH42</accession>
    <name>A0A1E5RH42_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Origin recognition complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1200</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">ORC is composed of six subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ORC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH42">
        <property type="interactions" value="442"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH42"/>
    <dbReference type="InParanoid" id="A0A1E5RH42"/>
    <dbReference type="OrthoDB" id="3972524at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000199">
        <property type="entry name" value="Origin recognition complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041083">
        <property type="entry name" value="AAA_lid_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048867">
        <property type="entry name" value="WHD_ORC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF23">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17872">
        <property type="entry name" value="AAA_lid_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21312">
        <property type="entry name" value="WHD_ORC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82061">
        <property type="entry name" value="BAH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="12">
        <location>
            <begin position="549"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="307"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="461"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="319"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="336"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="347"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="13">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ86228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="999" mass="113584" checksum="C9BD60D0C9EE83E7" modified="2017-01-18" version="1">MSFKHINFLLDWDVVGENGKPVLDVNTDSPSKRTRRSKRLHQEEHNELKKVVHKKNGLELTIGSCVKTTDKDANEMLILICDIRXDVLNKVAEVIGCHFEKKKSESLKQEEPVNSSEQAEAEHELVLTIKFFLLDSTANYHPITVLDKSENTLNYEETIKEVDSTIFYTKCIERNQVFLNFSLKNFVEKIRAVAKQGEPVLLNEVFHKMFSKGSEREKVDVSKVNIESSTKSLSKSHGSPVKMEESDFNDKKIEFTALRGSGDSQVDLLEGHSFEKNGVSLEDSLSFNTXGNKKTKVLSEVVLNQDSSEKDIPTDGEIASSDDYAASDSGFSSEKSDDEQNDSDASDDYKPVRKSTPKDLSFEPANSVFQKFNVTKEDYDDILKVYRTKKNNGKLSSSSKGKPKIKLNDAIKKYSRKHVGCAKKSHTPFSKRFRNVKAIPDLSNISDFHHLKVRETRTSLNQRFTSPRKKRITTGEGQPDGDNMDYGVETIYSKLKNTLVSRALNAEQKQEQIRNMIYEDNDIEDTIPGREDEYSRLFLSVYSSLESEQGTSIYVSGTPGVGKTLTIRQVIKDLYEPSLAEDGELPMFQYIEMNGLKFISSSDCYEVLWQNISGETLTTNAALQSLEYYFHDVPKNKKRAMIVLLDEMDTLVNKKDQDVMYNFFNWPTYKNSKLIVIAVGNTMDLPERCLSNKVASRLGLTRVQFTGYTYAQLQKIIGMRLKYINNCWFYVDQENGKAILSTHKDVQKXLTQPGSQNKKYKKVKLAITDNAVEIVSRKIASISGDARTALKICSRGIEIAEQEYLNKHGYDIYKLAAHHDTQRESGAINTAVFDDLEEENKTVAEEVYHIDIKHVLRATNETNNSPIIEYINNLSFAGKLILYAFISLKERNGSEEQQLGDVVDQVKTLIDLNTGTVSVQSSGSNGFKGTKNKYMDDIGRLLYVVEDGRPELRIADWEYLINELIDSDILVRNNILNERVQLYRLNMSFEELKKCLKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH72</accession>
    <name>A0A1E5RH72_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carboxypeptidase Y inhibitor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1607</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphatidylethanolamine-binding protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RH72"/>
    <dbReference type="FunCoup" id="A0A1E5RH72">
        <property type="interactions" value="1433"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH72"/>
    <dbReference type="InParanoid" id="A0A1E5RH72"/>
    <dbReference type="OrthoDB" id="2506647at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030414">
        <property type="term" value="F:peptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030162">
        <property type="term" value="P:regulation of proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046578">
        <property type="term" value="P:regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00866">
        <property type="entry name" value="PEBP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001858">
        <property type="entry name" value="Phosphatidylethanolamine-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF148">
        <property type="entry name" value="CARBOXYPEPTIDASE Y INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01220">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="223" mass="24815" checksum="A81599DCB37E64A1" modified="2017-01-18" version="1">MNQALQVPQTIKEAIEKHEIVPDVFSTDFNTSNLPNSLLTVEYPGELGVTMGNVLPLNKVQQAPDFQCLAIPSTETNFEIDLHEVYTLIMTDPDAPSRKDHKWSEYCHWIITGLTAEKANAQINVIKKNSTKKGELDQEIATARELVPYTPPAPPKGTGPHRYVFALFKGALNGSSSDNLIKQINENRANWGYNMPTAGVEKFANDHGGLELLAVNFFFCEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH75</accession>
    <name>A0A1E5RH75_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LSO1/LSO2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1119</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RH75"/>
    <dbReference type="STRING" id="56408.A0A1E5RH75"/>
    <dbReference type="InParanoid" id="A0A1E5RH75"/>
    <dbReference type="OrthoDB" id="4069860at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054413">
        <property type="entry name" value="LSO1/2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22048">
        <property type="entry name" value="LSO1_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LSO1/LSO2" evidence="2">
        <location>
            <begin position="11"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="94" mass="10847" checksum="24B2293DC2A39512" modified="2017-01-18" version="1">MGKRFSESAPKKAAGLQRKAEQAKQKSFQEKARLEQEEAARWSEGAPEPNKKKLEEQMKKEEKQRKKDERDRLLAEEEAELSAGKKAGKSYKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHB0</accession>
    <name>A0A1E5RHB0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein mS33</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1101</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHB0"/>
    <dbReference type="FunCoup" id="A0A1E5RHB0">
        <property type="interactions" value="225"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHB0"/>
    <dbReference type="InParanoid" id="A0A1E5RHB0"/>
    <dbReference type="OrthoDB" id="2257454at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013219">
        <property type="entry name" value="Ribosomal_mS33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13362">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13362:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08293">
        <property type="entry name" value="MRP-S33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="85"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="89"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="113" mass="12617" checksum="286C0E7BBCFADF19" modified="2017-01-18" version="1">MSSPLKLKALKIAELSAKVFDQNFNPSCARAGSKYLSQRLKGPAVVQYYGDPDFPKGTHIKKRIEEDLGYPHVKLFNSDEQYRVDAAMRKRKRGKGAPPKKKAPAASGNKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHB4</accession>
    <name>A0A1E5RHB4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1219</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHB4">
        <property type="interactions" value="113"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHB4"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="unsure residue" description="I or L" evidence="1">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="1">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ86277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="405" mass="47128" checksum="8A8036AAD7D0DADF" modified="2017-01-18" version="1">MDINSDGRLELVLTNKENLLNKLYKDQTLINNYKAALLNEYPIDDDGKQENSQYDPNDDERVIEDRFDVPSLKQIRTTITILLQDMCILSEKCLSHDDPDFSLNLDXQSFSSILREVKLKEQEFQTLQENERPKLLNLDELSTPXSNTLSXEESLPPNFITKEXLRLDNIEEMLNSYLTLYSESFNQNSSPDELDLISRVKMLSSIDQHIKHNVVTLQAKIKSIQNIQTTLIDKELLSVKEIYQNSIISSYENKIAQHXRYLNDLISKNFQSFTEQLQFLNVEELFAYIKKLLDIKISALKDYINKEKTIANNVADNLAXWQLFLKKIDTFEMELLSCLSNNXKSTSKLTKAAPSSTDQKIKQLFDEHIDFIETEIVQVCKKEKLNIIQYVEQELRFIKLTRQSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHN4</accession>
    <name>A0A1E5RHN4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cysteine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cysteinyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g1550</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHN4"/>
    <dbReference type="FunCoup" id="A0A1E5RHN4">
        <property type="interactions" value="930"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHN4"/>
    <dbReference type="InParanoid" id="A0A1E5RHN4"/>
    <dbReference type="OrthoDB" id="438179at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00672">
        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22249">
        <property type="entry name" value="UDM1_RNF168_RNF169-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00041">
        <property type="entry name" value="Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015803">
        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890:SF3">
        <property type="entry name" value="CYSTEINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="CYSTEINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00983">
        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA synthetases class I catalytic" evidence="13">
        <location>
            <begin position="43"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="729"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="672"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ86397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="771" mass="88190" checksum="6966F1F54AD2475D" modified="2017-01-18" version="1">MSSTVSTKVTQPKWHQPGNSTNEPVLKLLNTLTRNKDVFIPQSGGKRVTWYSCGPTVYDASHMGHARNYVSIDINRRIMQDYFGYDISFVQNVTDIDDKIIIRARQTYLFKKFLQEQNASEKEESVTQFPDESILKSLNEYLLTFIESNLKIKATSIQDYEKWATINKPNIETLKIESPKLPMYITSIDNAVKALSSPKNTVEFFQQVKDILVPALDSQFGSEINDPKVFQALPQHWEHEYDKDMRTLNVLPPDITTRVSEYVPEIVDFVKKIIDNGYAYPTSDGSVYFDTAKFDSSNKHDYAKCQPWNKGKLDLIQDGEGSLSNSVQDTKKSANDFALWKSSKPGEPKWSSPWGEGRPGWHIECSVMASDIHGATMDIHSGGIDLAFPHHDNELAQSEAHYDNCQWVNYFLHTGHLHIEGQKMSKSLKNFITIDEALSKYTWRQMRLCFALVQWNNQLDFKESLIQEAKGLESSFNNFFKNTRALNNDFKHAVANGVYVSKKTGNLEKKLLTDLYAVQDAVHVAFCDNLSTPVAIKNLAELVTKTNTYISTVGSDLKIEYVLNNVKYVTKILNTLGFTARPDGLGWEDVAEVSNKRPASLSNTASAEDTVLPYVKALSTFRDQVRQYAISKAPYGDFLILSDKVRDDDLLKLNVSLDDRSDNQGALVKFLNDQEREELLQQVNERELKKREKEQKKLEQARLRELKERERKEKASLKPQDMFKNTELYSAWDENGMPTKDKDGNEVTKSMTKKLKKQWDAQKKLHDEFFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHR6</accession>
    <name>A0A1E5RHR6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Carboxypeptidase</fullName>
            <ecNumber evidence="11">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1027</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Release of a C-terminal amino acid with broad specificity.</text>
            <dbReference type="EC" id="3.4.16.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="11"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHR6"/>
    <dbReference type="FunCoup" id="A0A1E5RHR6">
        <property type="interactions" value="858"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHR6"/>
    <dbReference type="InParanoid" id="A0A1E5RHR6"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046938">
        <property type="term" value="P:phytochelatin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031638">
        <property type="term" value="P:zymogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.410:FF:000001">
        <property type="entry name" value="Carboxypeptidase Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF113">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE CTSA-4.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11 12" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 11" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009028107" description="Carboxypeptidase" evidence="11">
        <location>
            <begin position="26"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ86424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="583" mass="66552" checksum="6C3A200950C8224A" modified="2017-01-18" version="1">MQLPLTSSSLLLLPLMTLLLNSVSAEHQDAFKMNYDNVNELKNNEIMNSKNNNKNNNNPTSDHRFQLLDEIMIKLGFATDDIGVSSSSSFNNNNNNDDDERSKKNPFFPTCLSDLNFKVESHLKKNDKKFTKTQRESEFTVKNVEKLGDNYQLRVNSIKNPEKLGVEVLADQYSGYLDVEDEDKHFFFWFFESRNEPSKDPIILWLNGGPGCSSLTGLFFELGPSSIGPDLRPIHNPYSWNKNASVIFLDQPVNVGYSYSSNSISNTVSAGKDVYAFLELFFQKFPQFLQNEFHIAGESYAGHYIPQFAKEILVDHPERSFELQSVMIGNGLTDPLNQYPYYHDMACGVKSGYKSVLSEDECDNMLDTLPRCLSLIEACYSTESVWTCVPASLYCNNAQMGPYQQTGLNVYDIRKPCVGELCYEDMKYIDEYLNQDYVKKAVGADENIEQYQSCNMEINQNFLFQGDWMKPIFKKDVQTLLDLELPVLIYAGDKDFICNWLGNLGWTNELEYADHTEFSSKDLEAWFVDGEEAGEFKNAGYLTFVRNYDGGHMVPYDQPEAALAMIQSWTLKGQKIEKNGTVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHR8</accession>
    <name>A0A1E5RHR8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA primase large subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1319</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the eukaryotic-type primase large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHR8">
        <property type="interactions" value="939"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHR8"/>
    <dbReference type="InParanoid" id="A0A1E5RHR8"/>
    <dbReference type="OrthoDB" id="421393at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07322">
        <property type="entry name" value="PriL_PriS_Eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058560">
        <property type="entry name" value="DNA_primase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016558">
        <property type="entry name" value="DNA_primase_lsu_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007238">
        <property type="entry name" value="DNA_primase_lsu_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10537">
        <property type="entry name" value="DNA PRIMASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10537:SF3">
        <property type="entry name" value="DNA PRIMASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04104">
        <property type="entry name" value="DNA_primase_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26466">
        <property type="entry name" value="DNA_primase_lrg_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009449">
        <property type="entry name" value="DNA_primase_large_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9 10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0408">Iron</keyword>
    <keyword evidence="7 9 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9" id="KW-0639">Primosome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA primase large subunit C-terminal" evidence="11">
        <location>
            <begin position="316"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="464"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR009449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR009449-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ86123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="503" mass="59467" checksum="66D3E0EEA5C0DA6B" modified="2017-01-18" version="1">MFRQTKRKLVNRRNLXEEDPTIKPEYLLQNVHTSGDVAFQENYFKESFSTDPYANLLSMYERPPIGEIXLEEFEVWAIDRLKLLLELESYHQRNRSIKEMIEQNIFKGLVSKAFSPGNTNYNFRKDYYSHHILRLCFCSNNDLRNKFIRDEILLFKLRFENMNSSDQEKFIATCPILKGKNIETVSQEELTDSMQENLFRVYGNKKINNKVIKLPFEDVIEMVGQRQVYLYKGFAYLPESLQMSYIVSEYQKFLESELLYTFRSLPRLNEDDRLLPILNHLGSGYMISDYNEQNSALSASDSNLTHSNVYASENFENYPLCMQNLLQGLKLNSHLKYNGRQQLSLFLKSCGLGIDEAMKFWENSFAKKYPIEKFNKEYRYSFRHNYGMEGNRINYRPWDCATILSKPRPSKEEFHGCPFRDWNEDRLRSTLKQKKDPSTGENLTNQQVNSVVDSCKQNDYTIACTKYFELTHATLHNEEQITHPNLFYERAVMSHKKEEEIDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHX9</accession>
    <name>A0A1E5RHX9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Histidine biosynthesis trifunctional protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="19">Phosphoribosyl-AMP cyclohydrolase</fullName>
                <ecNumber evidence="19">3.5.4.19</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="19">Phosphoribosyl-ATP pyrophosphohydrolase</fullName>
                <ecNumber evidence="19">3.6.1.31</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="19">Histidinol dehydrogenase</fullName>
                <shortName evidence="19">HDH</shortName>
                <ecNumber evidence="19">1.1.1.23</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="22" type="ORF">AWRI3579_g1621</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>1-(5-phospho-beta-D-ribosyl)-5'-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:20049"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="EC" id="3.5.4.19"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 19">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-5'-AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22828"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="3.6.1.31"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 19">
            <text>L-histidinol + 2 NAD(+) + H2O = L-histidine + 2 NADH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:20641"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/9.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 3/9.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 19">In the C-terminal section; belongs to the histidinol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.19" evidence="19"/>
    <dbReference type="EC" id="3.6.1.31" evidence="19"/>
    <dbReference type="EC" id="1.1.1.23" evidence="19"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHX9"/>
    <dbReference type="FunCoup" id="A0A1E5RHX9">
        <property type="interactions" value="384"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHX9"/>
    <dbReference type="InParanoid" id="A0A1E5RHX9"/>
    <dbReference type="OrthoDB" id="1703565at2759"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00007"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004399">
        <property type="term" value="F:histidinol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004635">
        <property type="term" value="F:phosphoribosyl-AMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004636">
        <property type="term" value="F:phosphoribosyl-ATP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06572">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11546">
        <property type="entry name" value="NTP-PPase_His4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.810:FF:000002">
        <property type="entry name" value="Histidine biosynthesis trifunctional protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1980:FF:000050">
        <property type="entry name" value="Histidine biosynthesis trifunctional protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1980:FF:000001">
        <property type="entry name" value="Histidinol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1300:FF:000002">
        <property type="entry name" value="Histidinol dehydrogenase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1080">
        <property type="entry name" value="MazG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.810">
        <property type="entry name" value="Phosphoribosyl-AMP cyclohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01024">
        <property type="entry name" value="HisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008179">
        <property type="entry name" value="HisE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016298">
        <property type="entry name" value="Histidine_synth_trifunct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001692">
        <property type="entry name" value="Histidinol_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012131">
        <property type="entry name" value="Hstdl_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021130">
        <property type="entry name" value="PRib-ATP_PPHydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002496">
        <property type="entry name" value="PRib_AMP_CycHydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038019">
        <property type="entry name" value="PRib_AMP_CycHydrolase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00069">
        <property type="entry name" value="hisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03188">
        <property type="entry name" value="histidine_hisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256:SF2">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS TRIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256">
        <property type="entry name" value="HISTIDINOL DEHYDROGENASE HDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00815">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01502">
        <property type="entry name" value="PRA-CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01503">
        <property type="entry name" value="PRA-PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001257">
        <property type="entry name" value="His_trifunctional"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00083">
        <property type="entry name" value="HOLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101386">
        <property type="entry name" value="all-alpha NTP pyrophosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141734">
        <property type="entry name" value="HisI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00611">
        <property type="entry name" value="HISOL_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 19" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="13 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16 19" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="11 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="15 19" id="KW-0520">NAD</keyword>
    <keyword evidence="10 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phosphoribosyl-AMP cyclohydrolase" evidence="21">
        <location>
            <begin position="148"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00008260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR001257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF01502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="OEJ86497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="802" mass="88221" checksum="EC62384393ADA9CE" modified="2017-01-18" version="1">MGLPFAPVYTSVRELEENFPKYQYTANCILDFNNLSVAEILQFIIAQGKYLQDVYVKNVTEKDDIVALLNGGVSKIIVDENNAHKIDTATISETDCSSRFISSSAVEELHLCNSEQLARKYMSCLKTDREDGLFTSVVVDANNKCLGLVYSSFPSLLAALQTGTGVYYSRSRNEIWYKGKTSGDTQKLLSVGFDCDGDCLKFVVEQSGSGFCHVPEQTTCFGSQNYDVYELEKIIQSRFQDSNLPSNSKSYTKRLFSEPKLLNAKIREEADELVEAQSKDEISWEFADLLYFSICKLVQNGVSLKDVEANLKLKNLKTIKRKGDAKAKYLETEEKKETENVEKKKPVEQDKKNGSVQKPIHLSVVNYGDKQGILDAFTRPIQKSNDIMNLVGPIVSNVRTKGDAALLSYTEKFDGCKLSSPVLEAPFPPEYIESLSEEMKQSLDLSIANVEKFHQAQLQTDILDVETQPGVICSRFPRPIEKVGLYIPGGTAVLPSTALMLGVPAKVAQCKEIVFATPPNRQTGKISGEVVYVAEKVGCKKIVLAGGAQAVAAMAYGTESVPKVDKIMGPGNQFVTAAKMLVSNDTSALCSIDMPAGPSEVLVVCDKKSDYDFVASDLLSQAEHGIDSQVILVGINLDQEYLTNLQNAVDKQANNLPRVDIVRKCIEHSSIVLCKSVEEAFEMSNNYAPEHLILQIENAESYLDLVDNAGSVFVGTYTPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQKFITSQAITPQGLDKIGKAVMTIAKVEGLDGHRNAVKIRMQKLGLVPETFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHZ0</accession>
    <name>A0A1E5RHZ0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein lunapark</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1173</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHZ0">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHZ0"/>
    <dbReference type="InParanoid" id="A0A1E5RHZ0"/>
    <dbReference type="OrthoDB" id="3972114at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="144"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="271" mass="31225" checksum="C54967C5F6441DA2" modified="2017-01-18" version="1">MSIISRLFTRKDDLTRYSVQLSKLTEKIDQTNKKLAKNNKHNHYYKLFIFSIISILLSSAIQWAVPXLLSSKMKMVLNFXTLVWPFIYYKFLQVRHNVLLKRLTVLREKHNTTLEDLKNKTSFYNTAAIINRYSAGQYGDQDEAKILDQEVLQKQEQLNEIRKELQENSKQSKEETDKWFDKCLNALAGGDTLVGPLPHQNAGNLDPINSQTMIRIICPQCQYDSGMYRVKDDPXCVFNCXHCAFNGTLDGSQKTNXALKALEPPENADKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI38</accession>
    <name>A0A1E5RI38_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">1-acyl-sn-glycerol-3-phosphate acyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.51</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1296</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:19709"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57970"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.3.1.51"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="4">The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.51" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI38"/>
    <dbReference type="FunCoup" id="A0A1E5RI38">
        <property type="interactions" value="286"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI38"/>
    <dbReference type="InParanoid" id="A0A1E5RI38"/>
    <dbReference type="OrthoDB" id="202234at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07989">
        <property type="entry name" value="LPLAT_AGPAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004552">
        <property type="entry name" value="AGP_acyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00530">
        <property type="entry name" value="AGP_acyltrn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10434">
        <property type="entry name" value="1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10434:SF11">
        <property type="entry name" value="1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="4" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="14"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="50"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="6">
        <location>
            <begin position="77"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="324" mass="35950" checksum="501BBCAE52291A09" modified="2017-01-18" version="1">MPLLSTLKYHLRSITSVILLGACAIYGVVISCVFNVIGQKRLSQWSTARCFNLIMGGILGISFKVINEHYLNESAPCIFISNHQSTLDILMLGRTFPKGCTVTSKKSLKYVPFLGWFMSLSGTLFLDRANRDKSVNTLNKGLQRLVKEKGSLWIFPEGTRSRTTELKMLPFKKGAFWLAKEGKIPVVPVIVSNTSTINNAKLKIFNRGEITVKILAPESLENIETKEQMNEFVARIENEMNDELKNNVGYSTPIDSTGVPQEYLDWLAKKQACDSTTDVETESVDVIDANVSANDEAVTSDNESTAVSADVEVHNITVSSSRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI78</accession>
    <name>A0A1E5RI78_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]</fullName>
            <ecNumber evidence="10">1.3.1.88</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AWRI3579_g1182</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>5,6-dihydrouridine(16) in tRNA + NAD(+) = uridine(16) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53380"/>
            <dbReference type="Rhea" id="RHEA-COMP:13543"/>
            <dbReference type="Rhea" id="RHEA-COMP:13544"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:53382"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>5,6-dihydrouridine(16) in tRNA + NADP(+) = uridine(16) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53376"/>
            <dbReference type="Rhea" id="RHEA-COMP:13543"/>
            <dbReference type="Rhea" id="RHEA-COMP:13544"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:53378"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>5,6-dihydrouridine(17) in tRNA + NAD(+) = uridine(17) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53372"/>
            <dbReference type="Rhea" id="RHEA-COMP:13541"/>
            <dbReference type="Rhea" id="RHEA-COMP:13542"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:53374"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>5,6-dihydrouridine(17) in tRNA + NADP(+) = uridine(17) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53368"/>
            <dbReference type="Rhea" id="RHEA-COMP:13541"/>
            <dbReference type="Rhea" id="RHEA-COMP:13542"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:53370"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 5,6-dihydrouridine in mRNA + NAD(+) = a uridine in mRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69851"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:69853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 5,6-dihydrouridine in mRNA + NADP(+) = a uridine in mRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69855"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:69857"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Dus family. Dus1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.88" evidence="10"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RI78">
        <property type="interactions" value="699"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI78"/>
    <dbReference type="InParanoid" id="A0A1E5RI78"/>
    <dbReference type="OrthoDB" id="272303at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017150">
        <property type="term" value="F:tRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082">
        <property type="entry name" value="TRNA-DIHYDROURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082:SF5">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(16_17) SYNTHASE [NAD(P)(+)]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="18">
        <location>
            <begin position="33"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="420"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="429"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="19">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OEJ86602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="439" mass="50884" checksum="16C588EBA0A01AAE" modified="2017-01-18" version="1">MTGEKPVKDLTKMNNLMSGRQLFKYLGEPKKIVAPMVDQSELAWRMLSRKYGADLCYTPMFHAKLFATSEKYRQDMWQDDLDGNPKFDRPLVVQFCANDPQYLVEAAKFVDGKCDGIDLNLGCPQGIARKGHYGSFLMEDWNLISELLSNLKKEITKTNVTCKIRIFPELEKSLEYAQMCVNSGAEWLTVHGRLREQKGQITGLADWNYIKHVRENIQCMTCDEKQVVFANGNILYSEDIHRCLNETGCDAVMSAEGNLYNPGIFNFSNNNDIQDKDKVFPRVDLVTREYFEILRKLDKKSKASMHAAKSHLFKILRPFFAKHTDLRNKLGSLNIMKLSFEEIENQLIKPIEETVAKILEEXPHLDVIEYDTEISNDVFQYKKVPYWRCQPYFRSVNGVTTNKRVGEFVESRDNKQEFLDNIKKRKKSKESTEDKDTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI95</accession>
    <name>A0A1E5RI95_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein SKG3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1597</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI95"/>
    <dbReference type="FunCoup" id="A0A1E5RI95">
        <property type="interactions" value="223"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI95"/>
    <dbReference type="InParanoid" id="A0A1E5RI95"/>
    <dbReference type="OrthoDB" id="5563754at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058155">
        <property type="entry name" value="Skg3/CAF120-like_PH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25381">
        <property type="entry name" value="PH_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="162"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="599"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="675"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="758"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="807"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="860"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="909"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="958"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1000"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1116"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1176"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="100"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="603"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="622"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="675"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="685"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="771"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="868"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="881"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="964"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1015"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1026"/>
            <end position="1102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1230" mass="137094" checksum="C08DBAC5B5A5AD8A" modified="2017-01-18" version="1">MRIFSKNKDKESPTESNHRNFLEVPPIVTRNSPASTKQSSRSDSPKTPTTPLSNLKNNSNTTDSFANFDPLMPPTITAKKRSNSFTNLLRAMSPSANSMNHYNNNNNYNNNASPSRESQLNQLSTANLMTKALSPELVPVVTLLSAQAHRRYHVGVFLIFYDLNNEGQPAERVWKEQYAVLIGTQLALWNASDLQISKNKKFTSKEMTEKLFNAASKPSYINFTDAKFKILPDRENTLVVSTTMKNRYFFKFSDKKSFHMWHATFRLCTFEYSSLQEAYTGAFLSSKGAKLSDINVILADTKFQYEDWVSVRFGAGMPWKRCYAVVSQPTRKPKKKKSKDHKKNHKNDKNDLEYNDEDDEFVYPQGAYGEVIFYENEKKIGNKKYAMARIHSAISVYAIYPSSPLLIDDSTMLKIEGMVTFGKDDKDPKESDIFIMPEKHFAVAGYDTIIRFLVPMLNAFYLYGRPKRLISTKEDPNSLLFGLPTLPEIHYLQVKDLLPLMEQNVSESWSNKIWKRKIKKLLLTKLKTGYSGCGSASGYSGALISPSITSVELFKKTASPQSQPISRFVSEGLPVSFTQKTEINSTAFHNLKTPVTQTAPVNLSGSSAKSKQSLSPIKRELATSNTQTKTSPNGVTTTTTTTTTETAYAKTPLKAVKSHQKHANQPTKKLETKFYSPQKSTVPIQSPSSPYQQYKSNHSGSSIFNPEIELNSVSASQQNTATQKALTPLKNQHQVLNQHTILSNTNSLQELDIGRESETSADSIVLTQPRADPKSDFSEARKQHASMNSEFLQNSIIDSYQHLQNTGAKVDDKKTGKKGNHNLDDDEDEDKDYVIGDNGSADPLADFYNLSRTLSSYKPLTSQTAQKKMSEEKPGIEHNSRNTTRPELTHQASSTILDDFKDARDSFDFGSSNSNNADDFENGNGFEADEEKNVFDPDYYEASSILKDESVNEIINEGGPAKQGSYSATESTHLNKSPSMRDLALPKFVAEDATTAFKKSPKVNDFKSQMNGPKPDFHQPTHQDQRTPTYTNQPQFSSTNRSASPLQPMINVNTGTHPSPGPQASQGSYGNPYQKQSPSQPYMNVTPTQPYHQPQFTNNSPRNQHQFAVNQYQNTGTGTTYQQMHPQRPAPPTAMAGGPVPGPSGYGYSPNARVMNPNNGGMPVTNNFTYQHPNVGKPVPPPMQMSRPMHSPTSPHFKKPSVKPAPLKKSGPGGFAQFMPPTPTTNPYSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN43</accession>
    <name>A0A1E5RN43_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphopantothenate--cysteine ligase CAB2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g873</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN43"/>
    <dbReference type="FunCoup" id="A0A1E5RN43">
        <property type="interactions" value="780"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN43"/>
    <dbReference type="InParanoid" id="A0A1E5RN43"/>
    <dbReference type="OrthoDB" id="70224at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10300">
        <property type="entry name" value="CoaB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035929">
        <property type="entry name" value="CoaB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12290">
        <property type="entry name" value="CORNICHON-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102645">
        <property type="entry name" value="CoaB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="240"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="381" mass="42942" checksum="9DE160ABCA3A177A" modified="2017-01-18" version="1">MISPSHRFGSESPIHTSVTEYHTAIDHGSDKNVVNADTLIEDEYFRTNPKPEYLDKLVGKAEKFLKNIPKTCKIVLLTSGGTTVPLENNTVRFIDNFSAGTRGASSAEQFLANGYYVIFLHREFSLTPFNRQFTHSATTQFLDYFNEHGELKQDFKAKILEEKKLQLKYQKQLLLLPFTTVNQYLWSLRSIALLMNNKNCLFYLAAAVSDFYVPYSKLPQHKIQSSNEYKKSDYSKESKSASSSSSSSSSSSSSSSSSSSGSSSNVLSSGKLILDLDPVPKFLRRLVQSWAKSSMIISFKLETDSSILIKKAKQALKNYNHQLVIGNLLQTRNKEVVFVSEDANIKEQWVKLTADVTCIEALIIPEAIKRHQEWIATQESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN77</accession>
    <name>A0A1E5RN77_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Adenine deaminase</fullName>
            <shortName evidence="7">ADE</shortName>
            <ecNumber evidence="7">3.5.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Adenine aminohydrolase</fullName>
            <shortName evidence="7">AAH</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">AAH1</name>
        <name evidence="9" type="ORF">AWRI3579_g644</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the hydrolytic deamination of adenine to hypoxanthine. Plays an important role in the purine salvage pathway and in nitrogen catabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>adenine + H2O + H(+) = hypoxanthine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:23688"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family. Adenine deaminase type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.2" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN77"/>
    <dbReference type="FunCoup" id="A0A1E5RN77">
        <property type="interactions" value="632"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN77"/>
    <dbReference type="InParanoid" id="A0A1E5RN77"/>
    <dbReference type="OrthoDB" id="272271at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000034">
        <property type="term" value="F:adenine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006146">
        <property type="term" value="P:adenine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043103">
        <property type="term" value="P:hypoxanthine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009168">
        <property type="term" value="P:purine ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000039">
        <property type="entry name" value="Adenine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01962">
        <property type="entry name" value="Adenine_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006650">
        <property type="entry name" value="A/AMP_deam_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001365">
        <property type="entry name" value="A_deaminase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028892">
        <property type="entry name" value="ADE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006330">
        <property type="entry name" value="Ado/ade_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01430">
        <property type="entry name" value="aden_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43114">
        <property type="entry name" value="ADENINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43114:SF6">
        <property type="entry name" value="ADENINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00962">
        <property type="entry name" value="A_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00485">
        <property type="entry name" value="A_DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Adenosine deaminase" evidence="8">
        <location>
            <begin position="19"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="7">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ88335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="355" mass="40545" checksum="57719C765EC913E2" modified="2017-01-18" version="1">MTISYDLTPEVKEFLKELPKCEHHLHLEGTLEPDLLFPLAKRNNVALPQGFPQTVEELNEKYKQFDDLQDFLNFYYMGCQVLINECDFYELAWQYFLKVHKQGLKHAELFFDPQSHTERGISVDTVYNGFAKACSKAESELGISSKLIMCILRDCPLQKCQDLVEKEAAHLIKEGKVHGLGLDSAEKPFPPTLFTNIYASAKKLNPELRLTAHAGEEGDHTYVKNSIDFLKVERIDHGVNSVQSDDLLRELSANKTMLTVCPLSNIKLQVFQDLKELPLLKLLEFGVPFSLNSDDPAYFGGYILENYYAVHETFGFDYKIWGDIVINTIKGSWCDENRKTELISLTKSIVAKYTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN90</accession>
    <name>A0A1E5RN90_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP synthase assembly factor FMC1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g861</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN90"/>
    <dbReference type="FunCoup" id="A0A1E5RN90">
        <property type="interactions" value="81"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN90"/>
    <dbReference type="InParanoid" id="A0A1E5RN90"/>
    <dbReference type="OrthoDB" id="15893at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039196">
        <property type="entry name" value="Fmc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28015">
        <property type="entry name" value="ATP SYNTHASE ASSEMBLY FACTOR FMC1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28015:SF1">
        <property type="entry name" value="ATP SYNTHASE ASSEMBLY FACTOR FMC1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13233">
        <property type="entry name" value="Complex1_LYR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ88334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="169" mass="19594" checksum="9681E011C1DB9939" modified="2017-01-18" version="1">MSAVSFTPELKASYKNLVKSLVRSSRRSRIQQLEASQKKEIALLKYDLIKLNRLNLQSTDPKNMEKHSDTKKQIERLENSALENSKKLLFHPQISHLKELILTSTPSSDSTKHSNRIKHFKEVSDFLINQSEYDELVERYNPGLTMSQEEKVKRTAQKVGFEIPPERVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNH1</accession>
    <name>A0A1E5RNH1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g831</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNH1"/>
    <dbReference type="InParanoid" id="A0A1E5RNH1"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="305" mass="34243" checksum="6122C3C7BD4EE5C9" modified="2017-01-18" version="1">MAKKNNKKNNSSHSNTSVTPKSGSLKNSSKSPTPSPSSPSLSSEPGLLTSNGDLAIKTKDAGLELQHNVSQEEQLLFKEFYTSALEQYLETKNGNGALTIQKQNKTPCSMIKCVLKTIFKVSVALCLIYAIKLYILYQVKYYSLYYLLPPITYPLNKVRNYLITLFTIFVTKVLPTTMGNTIIDKMGKHAVASAPAVDFEKTVIKNAVAIDMCSLNTLHLLLKLIQLQIYSVLPVQAVEAINYMIHLIQLAMHNLHQRIIVGIHDIWVHYHPQSYQYFVSHMTCLLRHLGLFTKSSCEGVKKAFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNM2</accession>
    <name>A0A1E5RNM2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein NSG2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g767</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the INSIG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNM2"/>
    <dbReference type="FunCoup" id="A0A1E5RNM2">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNM2"/>
    <dbReference type="InParanoid" id="A0A1E5RNM2"/>
    <dbReference type="OrthoDB" id="205546at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025929">
        <property type="entry name" value="INSIG_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15301">
        <property type="entry name" value="INSULIN-INDUCED GENE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15301:SF3">
        <property type="entry name" value="PROTEIN NSG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07281">
        <property type="entry name" value="INSIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="222"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ88502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="300" mass="33109" checksum="6DB455EBBC05E0B7" modified="2017-01-18" version="1">MTQAEKDPITSKSYSNLITPQLYGLYANANDTIVEEEADRYMALVDSFLSAKGHDPTTYIKDMNTKNSGKSVSAEKKLPSVDEMESFLKTHNAKTGASVKASKNGQFCLYKIVKSTIVFAVVGVVYSKIINMFYDHHLLIHPAVVYVLEPFLNASLGKAAGLGVVLGGVLYVFDKILLQAVFTTDKDRVESVSTLIKLGYSLIGMFLGLRKIEWKSGKEASLMWFCLNLMIWLMFDFTKSLLVIGIAMGGVLGVMNSSLESIGGDSIIGELSSTSLFLFNVNFVFSTIMFFGKLARYLRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNM7</accession>
    <name>A0A1E5RNM7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g783</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNM7"/>
    <dbReference type="InParanoid" id="A0A1E5RNM7"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="444"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="544"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="594"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="338"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="594"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="626"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="671" mass="77288" checksum="BDF5B8578F4D01BE" modified="2017-01-18" version="1">MATVSSRQSPFSSSTNFFNDLLDSNNNTFIHQKKETNDQEEFCATRSLDPSIRKRPGTPKHTTFESALKKQPFVLSPEEKKRYSQLYNIDDENILQEIIIKRNKHNSSLIGLDQNSVNSKIYGHSNQNSYNNHIQNSNNSFIQNNGGSIQNEQFCINTPQHSSYHNHSKPTQGSSLMSTPALQLKTPTSNIQTVTVLSPTKQNNNNDNNNNDNNNDNNDSNDNNHNKPKEVDPTEKMNQYFQDFSKFHQEQEINHKKRMSGKIDIHGNQKQNQKQNQILSSNNTKKSKFHLHSFINKLRSVSGTSSKNDNGKQKNKSKMKKQFYSNSINRQKSKSFRGKNNTILSKNSSPSPKQPYWNSSPNFQDPQSLSAIIDFCQIPLSRISSNNSMTEKIYQTVNNIRAAQRHHIKPRYSLRYNKHYKLKNYSSISDLHIDLSDFANSTKNAPFTYHPKRKKTQKKNKTGTHTQTKKDKLQNLWNEYLTQVVFQRVQLRLQMEKEYNTSSSMETTSGSGEEVKQHSQDLTHLDMNNMPLFDMKRLRKYTSTVNSTTHNESSESEEAVHNNHNKSSSCITNEDLYSIQNIINEYIEQKRKSISATTSHKSNQSHSDHFSKHSKQASNDESIRKNTSTSGIYNTTSYNTTNHTISNMYSGMLDTTHQTTFIRKHDRKDAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNY7</accession>
    <name>A0A1E5RNY7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ribonucleases P/MRP protein subunit POP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g594</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNY7"/>
    <dbReference type="FunCoup" id="A0A1E5RNY7">
        <property type="interactions" value="135"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNY7"/>
    <dbReference type="InParanoid" id="A0A1E5RNY7"/>
    <dbReference type="OrthoDB" id="442863at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039182">
        <property type="entry name" value="Pop1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055079">
        <property type="entry name" value="POP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009723">
        <property type="entry name" value="Pop1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012590">
        <property type="entry name" value="POPLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22731">
        <property type="entry name" value="RIBONUCLEASES P/MRP PROTEIN SUBUNIT POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22731:SF3">
        <property type="entry name" value="RIBONUCLEASES P_MRP PROTEIN SUBUNIT POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22770">
        <property type="entry name" value="POP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06978">
        <property type="entry name" value="POP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08170">
        <property type="entry name" value="POPLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pop1 N-terminal" evidence="5">
        <location>
            <begin position="105"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="POPLD" evidence="6">
        <location>
            <begin position="619"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="domain" description="POP1 C-terminal" evidence="7">
        <location>
            <begin position="896"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="8"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ88590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="954" mass="109878" checksum="C238D8226227F5EE" modified="2017-01-18" version="1">MDKRSATNSNNNNGKKPGNNNNGKQPGNNKNQKSLNNNQRNKLDRIRNARSIRTQIVASGNTHKHRRGGGNGFKSSGSNNNKRGYNSIERDIFSKDGGLLKVPEFINSRMFEIKQLEYSMKKSAKSSSTRCFQSLPRVMRRRTASHNIKRIPKRMRNRALREMVKGDQHITKGEKLTAPKQHLSKKNLYKLKMSVKILRLITKSKFMKNALPLKYYQTNLPQKFQLRKIMKIMKAHVRASSTTTTPTGGANTSSSISTTFPFQKNTMNNQLGHTDVTGINRLVTGSVELGQLVKSYKYAKRQAVNVWLPTHVWNAKRSHMMNLYGKKICYTPTQKCYKRTHRLGSPQSAQSDGCLIEDTCYMGKCVIESTDPMTNLVKLFKSKNDDLKIKNNNIFVNKETEKLGSIEKLVWIEKPNKLLLILHPSIYEKFFNLFLETFSHEINNLQTLSIHDCQYSIGSINLMGSNALLNLVKTFRSFQGKEESAAKEDIDFEKFVQLGASLRDFQLLKPDFSMSLQIFDPRLLKKPQLPNNINKYLKKDINDLLVDLHNTNVQQASNKNDVLKELCNPLQRYASYKNKSTLKELSKRYRAAVGQNFVGDFQKDVTIPVHLYKNAWGNGFTLLLPYHWVLPLWYQLNKIPRTYHIGLKQIQQLKYEMNVMDFPNDHLDTVAGYLNLVLKSHYSKELWLKKPSSKRLNYEKLKNLHFDDNRIRVGEVGDPFQPDFVFLRFLYFGLKYIVRKYPDLYENEQNVSLILPNQTSGMDLQTNQLDAKTVYDLIVHYNKLHTTVNKNMDFGSPIVAVPFSNSNGSTDNKVDILQFWSQFNSWKLPCTPVSISMVSRGHPTDNARLYRINKQNWDIYWKHYAENDKFNNHGNNNLENLKNNVIVKSQYLPKVQDLIGFVNAGTFNLSVGKGTGTGFILADWNSDAYCDKIGLLRNVGTDDYKLVKYEMVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP35</accession>
    <name>A0A1E5RP35_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative WD repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g855</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP35"/>
    <dbReference type="FunCoup" id="A0A1E5RP35">
        <property type="interactions" value="722"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RP35"/>
    <dbReference type="OrthoDB" id="25131at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039328">
        <property type="entry name" value="WDR89"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22889">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22889:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="148"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="355"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="355"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="381"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ88637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="412" mass="46083" checksum="FB503A72AA5352F6" modified="2017-01-18" version="1">MSVVSNRSFGSNNWCLQFETLSDSSADQSGNYLLTSLSNGECHVLNKETLASVNVCKGWFSGKPISGMTSMKDGNTFALSSDKTIKIFDLRTDLQKNCATTITSMNAPIMSLTYDHDTLCYGTELKGVDAPIHLYRKGYYNTPYRSFLDSHHDDVMSLKFHPTDKHVLLSGSTDGYCNIYDLTQEEEEDALHQVINYQSVHSCGFLSPKRIFVLGHMECLSVHSLNDKSDELNEEKPLDFGDLREPLNCEYVVNLYPGFVATGKNSESSLKIWSLQNEQINTKQPIVELANVHGGEIIRDVFVQDDLVYTCGEDGQVNISKTPNSLHLPKSFWEYNTNNSAETNVFEGTTAEVDMSENADEQPDFEANVLEADLNADKPRSSKHKSKGKSKHKSKHKSANQSSSSSRLYKPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVD2</accession>
    <name>A0A1E5RVD2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Membrane protein TMS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g401</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TDE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVD2">
        <property type="interactions" value="523"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RVD2"/>
    <dbReference type="OrthoDB" id="5963193at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005016">
        <property type="entry name" value="TDE1/TMS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383">
        <property type="entry name" value="SERINE INCORPORATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383:SF9">
        <property type="entry name" value="SERINE INCORPORATOR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03348">
        <property type="entry name" value="Serinc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="394"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="434"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ90799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="465" mass="51868" checksum="8DD8FB4577583096" modified="2017-01-18" version="1">MGAIISLPFTTAATFISSFLGATVSNLLSSVFSKFDTSASSVATRIVYALVLLINSLVSWIAMSTNHSILYPSKTCVGLECGVFSVYRLNFALGLLHVALAITISSLARSAWKVKMQNSLWALKTLFYFFTLFASFKWLSNDFFIWFSKYISSPSGAIFIFIGLILLVDFAHEYAEVCVRHVEEDDENSTFWRRLLVSSTLGMYVCSLVMIVVMFVVFCGDSCTMNLSSAVVNVLLGVVVTLLSIAPKVQEHNPKCGLVQSSIVTVYCTYLTFSAMASEPDDKRCNPLVRSSGTRRASIILGSLFTFVAIVYTTTRAAGNSAFNTTEEDSYNIHLDGADNDLFGEEDRSQLRIQAIREAVEEGSLPESALNDIELRHEDGDSESLAGFRPSYNYVLFHVIFFLATQWITMLLTINVKELENGDFIPVGRTYFYSWVKIISAWLCYGLYAWSMVAPIIFVDRFXYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVD9</accession>
    <name>A0A1E5RVD9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pyruvate dehydrogenase E1 component subunit alpha</fullName>
            <ecNumber evidence="5">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g404</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVD9">
        <property type="interactions" value="658"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVD9"/>
    <dbReference type="InParanoid" id="A0A1E5RVD9"/>
    <dbReference type="OrthoDB" id="10256198at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000013">
        <property type="entry name" value="Pyruvate dehydrogenase E1 component subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050642">
        <property type="entry name" value="PDH_E1_Alpha_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017597">
        <property type="entry name" value="Pyrv_DH_E1_asu_subgrp-y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03182">
        <property type="entry name" value="PDH_E1_alph_y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516:SF60">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 5 7" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="6">
        <location>
            <begin position="97"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ90796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="430" mass="47141" checksum="BC41584B6874FDED" modified="2017-01-18" version="1">MLSVVARNQAFALKKAAAIXLSSTTARSFXKQTAIAAKQQAAGSDLSKAEEAAAATENDELVEITLPESSFEGYLLDTLPDLTFQTSKGTLLQMYKDMIVVRRMEMACDALYKAKKIRGFCHLVVGQEAIPVGIEYAATKRDTLITSYRCHGMTYMRGASVQAVLAELMGRRTGVSFGKGGSMHLYAPNFFGGNGIVGAQVPLGAGLAFSHKYKSEDACSFTLYGDGAANQGQVFESFNMAKLWNLPCIFCCENNKYGMGTAAARSSAMTEYFKRGQYIPGLKINGMDILAVYQGSKFAKDWAVSGNGPMVLEYETYRYGGHSMSDPGTTYRTRDEIQHMRSKNDPIAGLKMKLLELNIATEEEIKSYDKAARKYVDEQVQLADASPAPEAKMSILFEDVYIPGSEVPTLRGRISDDTWDFKKGGFAFRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVG5</accession>
    <name>A0A1E5RVG5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RVG5"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="432" mass="47692" checksum="1F095BE36CF88A15" modified="2017-01-18" version="1">MTLAFGEGELTLSTDTPMQNTFEQGPCAAIVRVAKNANTTNSKMKGDARAKTAKISNHNLHNEGEKLVANSTPFVCTRIPALDAGLAVETSALASMLPSSAHAQAMSWWPESYSKGDECETMEETLAACASRTETLXAXSVVYIGRDXSFVGLATTGTRASNFKICDSXLLPYRANXASCTKTXLFFSFFFHGSGXDRTHNHLDITNCGSRSDKHNGKKCAVXHGIDEAEVEAHENTQTLVTVKGRKNGHQQIVDHAPQSEASDRDTLLEMDRGAGKYKDGDTCSNNHNDHELKXDNTDXIESGXDTAGDRSKQIPEFSNTLTQKVLDEDLTIDELMNEVAAILRRDCGSTHDLKTAFCGSEDDEKTDIHEAIIARRYSXLKKREGVWAITRGEQEKDRIVTMTTWRKQNRRKVSMLNLCFEHVKTRTARTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVM1</accession>
    <name>A0A1E5RVM1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ATP synthase subunit g, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g501</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase g subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVM1">
        <property type="interactions" value="102"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVM1"/>
    <dbReference type="InParanoid" id="A0A1E5RVM1"/>
    <dbReference type="OrthoDB" id="437at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006808">
        <property type="entry name" value="ATP_synth_F0_gsu_mt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04718">
        <property type="entry name" value="ATP-synt_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4" id="KW-0138">CF(0)</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ90818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="113" mass="12480" checksum="6EB975E878CAA74A" modified="2017-01-18" version="1">MSAFVSNLSSKINVLVSKSIYYSKVVGEVAKQVYVKEGLQPPAFADVKSVYSTLFAKAVYYSKNTAEFTPILKNLNKNNYITYGAIGVQLAGLXNLGEIIGRRHIVGYKSHHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVM3</accession>
    <name>A0A1E5RVM3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">COMPASS component SWD1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g545</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVM3"/>
    <dbReference type="FunCoup" id="A0A1E5RVM3">
        <property type="interactions" value="968"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVM3"/>
    <dbReference type="InParanoid" id="A0A1E5RVM3"/>
    <dbReference type="OrthoDB" id="196858at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037850">
        <property type="entry name" value="RBBP5/Swd1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44040">
        <property type="entry name" value="RETINOBLASTOMA-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44040:SF1">
        <property type="entry name" value="RETINOBLASTOMA-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="88"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ90909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="522" mass="59048" checksum="26FA8E29E2BDD12E" modified="2017-01-18" version="1">MNLLLQDPFSVLKEYPENLANILETPLYTVIQKFSPNGDYLAVGCVDGTLIIYDMDTLKPIRILGSQRQKSNGHHEPQLEMFNGTHASVGHTQAIQSIQWSSNGRFLLTSSRDCTVKLWDLLHTNDASVTHTGNFHQCKSFTFDTFVWSASFLPGDTQCVVCVSTNALPFIIDFSTEVVCPIVDRERNATLVYDDCKKYGNSISNFVFEGNTQYASSLEEKPHSNDELLASTASGAVPNNVEKRNEEVNAERGFEKVLQKNIILVGTTKGWLTIYEMVGMNRIVFLESIKITSSSIKNIILSEAGNLLCLNGADRTIRQYALSFENQNDYNTEKETGNTQSLSNAKIALHFQHKYQDVINKLQWNSISLSPKYGEYLVASTHGSSAHDIYLWETSSGFLVRVLEDAGEELLNIDWNYHTMDIVSNGLETGNVYNWSIVVPPKWSALAPDFEEVDENVKYMEKEDEFDEYDEELERNDRNKLEEKDLDLVTCESFDVRGNAINTKIISIPLEYDKIVQLRNAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVT8</accession>
    <name>A0A1E5RVT8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Initiation-specific alpha-1,6-mannosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g523</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVT8">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVT8"/>
    <dbReference type="InParanoid" id="A0A1E5RVT8"/>
    <dbReference type="OrthoDB" id="409543at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039367">
        <property type="entry name" value="Och1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834:SF1">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="148"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ90858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="537" mass="62450" checksum="78550BD0970FBB1F" modified="2017-01-18" version="1">MALKTPFHWAQKRKSKRFLVLTTALVVFFYVVIVPLNKLPLVNRLGSLTTPMVLPSTYESSVNFKTGIDTSASKRGIENNDKSQKINEQSETANLRSLMNSHFPYDPYSSTPKHVWQTWKVKFDSPEFPPYYKTFSRSWQKKTLYNPLRDPMEQDPEAENYVHSPSSSASTLRKDEKDKQWVYSLIDDSEVESLIENIFYEVPAIVQAYKLLPTPILKADFFRYLILYSRGGIYSDMDTIPLKFLNTWPGNNPTYIKEKIYKNDVMHTYGIRYKNMDQTFENEMDKENLVQKQQLQPLTMTPGLVIGIEADPDRPDWAEYYARRIQFCQWTIQAKPGHPLLRELILNITATTLNTVSNKYVQXEYYSLEKTHVSESNTNLDKTDYFFRARGKQALNVDLKELAKTKNKVWKPEEIDGTDIMNWTGPGIFSDTVFNYMNNLLKYNKDINIVNKNLEYDDLEKTTKKFYKQVSTNLVDENNFHWGFFSLLQDPILIDDIVVLPITAFSPDVGHMGSLSSLEEIALAKHYFSGSWKNEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVV5</accession>
    <name>A0A1E5RVV5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">nicotinamidase</fullName>
            <ecNumber evidence="6">3.5.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Nicotinamide deamidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g524</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; nicotinate biosynthesis; nicotinate from nicotinamide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the isochorismatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.19" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVV5">
        <property type="interactions" value="420"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVV5"/>
    <dbReference type="InParanoid" id="A0A1E5RVV5"/>
    <dbReference type="OrthoDB" id="3341310at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008936">
        <property type="term" value="F:nicotinamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019363">
        <property type="term" value="P:pyridine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052347">
        <property type="entry name" value="Isochorismatase_Nicotinamidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11080:SF2">
        <property type="entry name" value="LD05707P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11080">
        <property type="entry name" value="PYRAZINAMIDASE/NICOTINAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="8">
        <location>
            <begin position="9"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ90863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="235" mass="26880" checksum="0EE7C25D69076A90" modified="2017-01-18" version="1">MPMHLPPNSCLLIXDMQYDFLPPDGSLAVPGGDSXVPKIRHLISENSKEFDLIVFTQDYHPQTHISFASNHKDTKPFDSIDYKNPXXISXPNKHEEEQDDKEVTLETIKSTLWPDHCVQGTRGCEIEESLLNTANKNVPYKLKFVKKGYLEDREYYSCFNDIWHSHHTELNETLKNAGIKHVYCCGLALDYCVFNSALSSAKLGYQTFIVKNMTAAIDKSWDLDKMNTNISYVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RW55</accession>
    <name>A0A1E5RW55_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g371</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RW55">
        <property type="interactions" value="1381"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RW55"/>
    <dbReference type="OrthoDB" id="3973009at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11388">
        <property type="entry name" value="bHLH_ScINO2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057071">
        <property type="entry name" value="bHLH_INO2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23179">
        <property type="entry name" value="bHLH_INO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="2">
        <location>
            <begin position="313"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="3">
        <location>
            <position position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ90978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="397" mass="44599" checksum="6E3FF3477FFC618C" modified="2017-01-18" version="1">MSLNSEAQDLFDLDIDFETAYNMLGNTDTFLSSFLNASPKKTEHIEPSNQHTSALFETTHXAQTSINSANTQXMQPQIQTQQFGQSLNNVSTSESNVHDGLNSKTFFSSKSQDTSDNDVSAPHHANLASFPSDIAQYNDHGNGNYIPETSNPDQRQRQPSTYELLSVNESHAIESFLDSLMEGNNQKNIAVAQQHVQRNSXPSHNSSSERLATPGDTHLKHEGSTQPFSSENIEARNVWGMEPQRFVNNVEHDASVSVKNTGPASTPKFSSPQPENDAFLTSMENSKEEENTDKLGYQELSVKYEKIMGQINVQKKKHLLAEQKRRAAIKNSFELLQNTIKYPRVDGFHARNSKKRVKKHEYLQFVIEDIEGLLRANQELREMNESIGNLSTYGSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RWE1</accession>
    <name>A0A1E5RWE1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein SPT2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g426</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPT2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RWE1"/>
    <dbReference type="FunCoup" id="A0A1E5RWE1">
        <property type="interactions" value="437"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RWE1"/>
    <dbReference type="InParanoid" id="A0A1E5RWE1"/>
    <dbReference type="OrthoDB" id="4035998at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013256">
        <property type="entry name" value="Chromatin_SPT2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08243">
        <property type="entry name" value="SPT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00784">
        <property type="entry name" value="SPT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="11"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="11"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="91"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="153"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="209"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="222"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="235"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="251"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="4">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ91033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="342" mass="39196" checksum="0354077B0036095B" modified="2017-01-18" version="1">MGYLDQLKAIQQNKKTQARGQTSGKAYSPLSSRNGSQSFKTSNAFTDPLDMKSSILPERYTREVDPAVKRLKELRRQKQLEEDAKRGGTLKVRKPSAARKARQSPTDKDSSITSTRFKKKIENKRQSMEVPQARTPAVKEKKLSFEQLMAQAEKSNCTNGADSSARSTPIPQKRAPVEISGWKKKKKNSPLGNTSTSVREKFKNAKPQESVKDVSEKQETLMSIPSSIKSGPSKKIMKKLEKRKSSRNGYYDDEENDSEFDDFIEEDDPGYDRSEIWKLMSNGRARSSRPYDYDDESDDMEADGFEILEEEEASRKYARLEDKKEEAWLKEHEKSKRKRLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RYW2</accession>
    <name>A0A1E5RYW2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">U3 small nucleolar RNA-associated protein 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g253</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000002">
        <property type="protein sequence ID" value="OEJ92131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RYW2">
        <property type="interactions" value="839"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RYW2"/>
    <dbReference type="InParanoid" id="A0A1E5RYW2"/>
    <dbReference type="OrthoDB" id="277439at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006709">
        <property type="entry name" value="SSU_processome_Utp14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14150">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14150:SF12">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 14 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04615">
        <property type="entry name" value="Utp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="138"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="416"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="601"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="60"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="109"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="157"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="418"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="429"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="455"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ92131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="875" mass="99699" checksum="DDB5E4C4054D5156" modified="2017-01-18" version="1">MAKKRVSRNHNKKRAQNALEIAERELYAGMSSSDEDDRRNNPRLNGTIVNPQKRAQGDASDAEGDDEDEDDFEDEEIDSDEAFGSDEEIDVLDSRFSQTIRDKLKNGEEIDXIDEEDGYTSIDEDDLLPLSAIWDANEKVEKNDSKSVSDNQSDLMLNDDDVSSASESEESESEDEEESDDPFDEVSDGEVDLKTVAKNLIDQTTSKQQQYKKLNNAAVGEENEYALPSLSNNTSTSLNLADILSVVDDPDAVNRASLIKKGENQAMAVPLPQRIQKRNERKAAYEISKDEVSKWQDTVQQNRRADHLDFSKLNEADAKVEASTFTPIEKPMTELEKKVDEVMKQSNLIDMTKKESTFEEIATAKMSPEEMRKRTAELRLMRDLMFREERKAKRIKKIKSKAYRRIKKKEMLKNQALVDEDEDEDEQSKEDRDIARAKERMTLKHKTSSKWAQDMIKHGMTKDKETREEMEDMLRQGERLKAKILGHESGEESDRDLDHLDSSDDENDTPETRSKVGKSGVLNMAFMKNAEARERERNLAEAEELKQLRNSNGLELFQQTSANKEAIDGANVHINAGRRVYTPGTAESNEEMKQLDDQVLQEREIDEQGTIEKRIAKKQLTNAQKKSSKKKNEDTTTENATEEANPWLDGSDDEDYQGQKSSKVYVVDENSSKLSKAASKISKNKAKNEAKKXVKKTNNDDLILDLESSNTLDIVDPYGGSDSEEFKQQDLIAEAFAGDDVVSKFEEEKKRVAIDEDDKEVDVTLPGWGDWAGEGMNPRKKRKFIKTVKGVVNKDKRRDKNLKHVIINEKVNKKNLKYQSSAVPFPYENREQYERSLRMPIGQEWTSSNTFSKAIKPRVVTKPSSVIDPLKQPFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RYY1</accession>
    <name>A0A1E5RYY1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">U4/U6 snRNA-associated-splicing factor PRP24</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g267</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000002">
        <property type="protein sequence ID" value="OEJ92151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RYY1">
        <property type="interactions" value="127"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RYY1"/>
    <dbReference type="InParanoid" id="A0A1E5RYY1"/>
    <dbReference type="OrthoDB" id="360390at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031766">
        <property type="entry name" value="RRM_occluded"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16842">
        <property type="entry name" value="RRM_occluded"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="37"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="117"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="400"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="403"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ92151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="431" mass="48912" checksum="EF2359EA473B87CA" modified="2017-01-18" version="1">MERLKRRLSTGETAVLSRKKPSSIDQLQELDNRRETTTVLVRNLPKSTNIGQIKHYFADCGAVTHVGFAKTLQIAKVQFAAYEDALSALTRTSKIFNLKDPISGKGNQITVEWYRNSTIWCTNYPPTYRIPDLNKLFEEAGGQVLNIRTPSLRYNANRRFAYVDLTNSEDAASCVAKLNGKILENNYSFVARLSDPSSKTKRTDQSLLDGRELLLKNIDGVDIPSLKLYLTTWGGEIERFTIPKNNQSIAWCSFTSSHDLQRLLAKSVTVKLDDKIVYLQKADKKAYLERQQVKELMNLRKRDPLYETFLSLFPLSDKYNKETVKSLILSKTSLLESDISNILLVSDHEGALIQLADASKFSKTILELQGKNLNKNVLLKCADIDALKKHCPNNKARINHVQEQSKQEPAKQDNHTIGGKTNNDFKXILYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZE3</accession>
    <name>A0A1E5RZE3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Endoplasmic reticulum oxidoreductin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">AWRI3579_g248</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">May function both as a monomer and a homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EROs family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000002">
        <property type="protein sequence ID" value="OEJ92135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZE3"/>
    <dbReference type="FunCoup" id="A0A1E5RZE3">
        <property type="interactions" value="779"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZE3"/>
    <dbReference type="InParanoid" id="A0A1E5RZE3"/>
    <dbReference type="OrthoDB" id="269384at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016972">
        <property type="term" value="F:thiol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007266">
        <property type="entry name" value="Ero1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037192">
        <property type="entry name" value="ERO1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12613:SF0">
        <property type="entry name" value="ERO1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12613">
        <property type="entry name" value="ERO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04137">
        <property type="entry name" value="ERO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017205">
        <property type="entry name" value="ERO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110019">
        <property type="entry name" value="ERO1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 17" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 20" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184952" evidence="20">
        <location>
            <begin position="22"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="16">
        <location>
            <position position="388"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="391"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="18">
        <location>
            <begin position="118"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="18">
        <location>
            <begin position="388"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR017205-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR017205-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR017205-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OEJ92135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="613" mass="70249" checksum="B244575A59F06598" modified="2017-01-18" version="1">MKLTGLVAPLLVLQQLYSVLAAENTNTNQSSNTKVNTTNNNNNNNNEVDQLSDNTNFCKVNKDDLISETCDVTFHEINEMNAKIRPILKKVVGSDFFKYFKLNLYTECPFWEDNNGYCVNRACAVDIIEDNWDQLPEYWQPDVLGQLNSSEESMIIGNQTDDVQEEFLDQLCKFNNLNGNNKFVNQKFERDIEYCDVDTLTNHENAVLVDLTANPERFTGYGGEQSGMIWGSIYRENCFSVTPEDKLGKDPKCLAKDAFYRLVSGMHASIATHLSNDFLDTKTGKWGPNLELFMNRVGYFPDRVANIYFNYAVVSKAVYKISGYLKNLEICEAYDENVTGMIDQISSDIDTRIFNENLLFQNDISSDLKDQFRTRFKNVTKIMDCVHCDRCRLWGKVQTTGYATSLKILFEMDEFDAEEIVNGLTKYELIALFNTYDRISKSVESINNFEKLYHQKNEGFFQRNNIFKLLDRAKKQWLTTSSSISSSITSTLKSLSTSADSEAPIKASGEQPAKEDIEEEKFQDLIMSSKKKAANSEPAKGDNRSSLRVAWDTEINNVKEALKFIFKSYIDLPKNMGYLLLRKMNSLWNSFIGVENYFDESDQNDNTYTLNIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZI7</accession>
    <name>A0A1E5RZI7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ubiquitin domain-containing protein DSK2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g281</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000002">
        <property type="protein sequence ID" value="OEJ92158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZI7">
        <property type="interactions" value="680"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZI7"/>
    <dbReference type="InParanoid" id="A0A1E5RZI7"/>
    <dbReference type="OrthoDB" id="267397at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14324">
        <property type="entry name" value="UBA_Dsk2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16106">
        <property type="entry name" value="Ubl_Dsk2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000024">
        <property type="entry name" value="Ubiquitin domain-containing protein DSK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006636">
        <property type="entry name" value="STI1_HS-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015496">
        <property type="entry name" value="Ubiquilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10677:SF3">
        <property type="entry name" value="FI07626P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10677">
        <property type="entry name" value="UBIQUILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23195">
        <property type="entry name" value="UBQLN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00727">
        <property type="entry name" value="STI1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="3">
        <location>
            <begin position="3"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="2">
        <location>
            <begin position="343"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="258"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="322"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ92158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="389" mass="41474" checksum="EE09DC561F425A11" modified="2017-01-18" version="1">MSESITVHVKSGQHKWEVRVVPTATIGELKTEISKISEIPSENQRLIFSGKILKDESTVESYKIADGHSIHLVKSAGKKTNTNSATNSATNSATTETXGTDSSNSTGTANGNENVPNSFATGSGSAFNPLADLTGARYAGYGLNLPSADSFGPDGGMNNMMNQEDMLQMMENPIVQSQINEMLSNPQMVDYLIEQNPVFRSMGPSARAMLTSPYMRQMMTNPQMIRQSMQMQNAMGRGNNPNAGNDFGFPSPGTAAATDNGSTTTTTNNNTTNTNTNTSTSTDNXNNTSNNNTVNPFASMFGNLPPPNPEFIQQMMSEGLNPGSAFGSGSSPSAAAAPADNRPPEEKYEHQLRQLNDMGFFEFDRNVAALRRSGGSVQGALDALLNGSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZV4</accession>
    <name>A0A1E5RZV4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g98</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZV4"/>
    <dbReference type="InParanoid" id="A0A1E5RZV4"/>
    <dbReference type="OrthoDB" id="3364892at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF26">
        <property type="entry name" value="CARRIER PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G07710)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="158"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="261"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ92414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="422" mass="47876" checksum="FAEC4AF9CDA095FB" modified="2017-01-18" version="1">MRELDFTSDYEDTANALLESTSSGHPSSFIAVLVSGLRYVVYRLTSFYLKTPLKLFRPARFDALFYLRLMEAQKLALRQNTQKITIPRTKTAAIRNALYNSSLGILIRSLRQHGWKILPQAVAPPFLANSVSGVVLYTSYLTFLPSPFFQSTTSEIKILPKDAFCSGALAGFMQAIVSQPIEAVYQRSTVSEFLSLTRMHQSLWKFGFDKLKQAGFLGVFYGGFSLSILKESLGFAVYFGSFEYLKYNGYDLLLPTKNHKETTLPSNQNEQKDPTSSTTVRDLSMRSEPRXGYLNRKTLQTFNAGVSAAFFLQVIQFPINKILKVXVSNLQMQSELNFNILTKSLKHNWSIYSSSYINTFKNIKALYTKEKCXFAAWSYKGFWRNTLAIIPGTTASLLALEYLRNYTRTQSLDHGELKKMTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S0C4</accession>
    <name>A0A1E5S0C4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">DASH complex subunit SPC34</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Outer kinetochore protein SPC34</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AWRI3579_g8</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex SPC34 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5S0C4"/>
    <dbReference type="FunCoup" id="A0A1E5S0C4">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S0C4"/>
    <dbReference type="InParanoid" id="A0A1E5S0C4"/>
    <dbReference type="OrthoDB" id="10016597at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013966">
        <property type="entry name" value="Spc34"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08657">
        <property type="entry name" value="DASH_Spc34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="16" id="KW-0137">Centromere</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OEJ92423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="286" mass="32786" checksum="FA12B28E42AA215F" modified="2017-01-18" version="1">MQRLQQCLDKLDKHNKSIATLYFKPPGIYNNAVSTINDDAENITSLIRDVHTEEETELYSVDEHQTVRKDGQRIGIMDYLSTLNSDSQTTGDMANMTSQGSKPVITVPMEYYLKQNELMLESANRSNRNKRRRTGTAALDEIFFSRLDENAFVFDILLKKFSNDSQMRSLIMALRDGSVLDEGAIASVGGEGANSGDHRRRTLFVEDFPSDCIFKIFQEIVTNWPLNEYNTKFNSLLERFNLISDQVNELKEHISMHEEDGVSIEQLIDKEEAEIEILKLQLQQMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R036</accession>
    <name>A0A1E5R036_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g4651</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000017">
        <property type="protein sequence ID" value="OEJ80246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R036"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ80246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="123" mass="14542" checksum="3B5900D76E567CF7" modified="2017-01-18" version="1">MAREQLDGAKLCFRVIYMCERVTQLSQTFVIMETTKVKTPQQHVIKQEAFAQXXAFSNGMMKPSXQYRVFIPEKNLLFKSEERVQYNGLGEQEESYVNVNKKRREARFTRLRSLHDDNRTVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0H4</accession>
    <name>A0A1E5R0H4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ribose-phosphate diphosphokinase</fullName>
            <ecNumber evidence="3">2.7.6.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4474</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">5-phosphoribose 1-diphosphate synthase involved in nucleotide, histidine, and tryptophan biosynthesis. Active in heteromultimeric complexes with other 5-phosphoribose 1-diphosphate synthases (PRS2, PRS3, PRS4 and PRS5).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0H4">
        <property type="interactions" value="223"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0H4"/>
    <dbReference type="InParanoid" id="A0A1E5R0H4"/>
    <dbReference type="OrthoDB" id="413572at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009156">
        <property type="term" value="P:ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000063">
        <property type="entry name" value="Phosphoribosylpyrophosphate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000056">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000842">
        <property type="entry name" value="PRib_PP_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF36">
        <property type="entry name" value="RIBOSE-PHOSPHATE PYROPHOSPHOKINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00114">
        <property type="entry name" value="PRPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="12">
        <location>
            <begin position="5"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ80390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="427" mass="46833" checksum="109ADF0143C603CE" modified="2017-01-18" version="1">MSNNIVVFGGSSHPELVEQICDRLSIKPSQVTLGKFSNGETSISVGESVREKDVYVIQSGCGHVNDVFMQLLILINACKTASANRVTAVMPYFCYSRQPDIPFTSKGAPLLPLASNPSSANLSDNKINGGSSSTSLNYIPVVENNNSNSESSEASFNISNSGYKLWIAQNGTLIANLLTTAGADHVITMDLHDPQFQGFFDIPVDNLYSRPLIERYIQFQIPNYKDAVIVSPDAGGAKRAAKIADALELSFAMVHKEKRCQLIKGPPSNPTVIASHSMRQQASINRKMQNNKYQKDSNSKYVSTTMLVGDVRNKVCILIDDMVDTSYTITRAAKLLKDQGCTKVYSIITHGVFSGDALDRISKNGDIDHIVVTNTVPQKEHQEKLKXLASLKKTPVFEILDVSAVFAEAIRRIHNGESISTLFERGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0J4</accession>
    <name>A0A1E5R0J4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">GPI mannosyltransferase 2</fullName>
            <ecNumber evidence="12">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4497</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the PIGV family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="12"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0J4"/>
    <dbReference type="FunCoup" id="A0A1E5R0J4">
        <property type="interactions" value="300"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0J4"/>
    <dbReference type="InParanoid" id="A0A1E5R0J4"/>
    <dbReference type="OrthoDB" id="10252502at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031501">
        <property type="term" value="C:mannosyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004376">
        <property type="term" value="F:GPI mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007315">
        <property type="entry name" value="PIG-V/Gpi18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12468">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12468:SF2">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04188">
        <property type="entry name" value="Mannosyl_trans2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 12 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 12" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="125"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="224"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="280"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="400"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="451"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ80397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="472" mass="55411" checksum="962E0D017390C178" modified="2017-01-18" version="1">MFSVFDMKQPPIWTITKVFWISKVLQYTIIFFTPCIESQFDTSTTLLLDNFPQLDQSQWYNAKILNKLLSWDSAYYLKNILHMAQTPDGIYLPEYENEYAFSPFVWSSVLRFSVKALLLKEDTPFLLLVLISITNIIHYCSTIALFYLTRIVFQKNSMYKYQKQTVFRIAMFTCLLFIFSLSGGFQSTFYSEQFSTLFTFLGMYTREYSTKYTGSFTITIENKALYLLTSVFFTVAAVNRPNCVINGLFFLLDLQKFIFNKTSQHSELKNKLNDHRKREALYPLFSGCFMLCGMVYTVYYTPFQIFCNTSNSADNLPNWCNNSLLSQLELLKLNSLVPTFVTRNSLYTYIQRRYWNVGLLTYYTANNIPNVLIALPNVIYLFMAANYFQWDFNSFNLHSYCYVSFAFLIMICGWAHLQIINRVITFIPLHLWYLSSCFAKNGNSLKVKLYLAWVIIWWPLQTVLFANFLPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R170</accession>
    <name>A0A1E5R170_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">5-oxoprolinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g4306</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxoprolinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R170">
        <property type="interactions" value="834"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R170"/>
    <dbReference type="InParanoid" id="A0A1E5R170"/>
    <dbReference type="OrthoDB" id="3643at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049517">
        <property type="entry name" value="ACX-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008040">
        <property type="entry name" value="Hydant_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003692">
        <property type="entry name" value="Hydantoinase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF2">
        <property type="entry name" value="5-OXOPROLINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19278">
        <property type="entry name" value="Hydant_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05378">
        <property type="entry name" value="Hydant_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02538">
        <property type="entry name" value="Hydantoinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase/oxoprolinase N-terminal" evidence="4">
        <location>
            <begin position="5"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase A/oxoprolinase" evidence="2">
        <location>
            <begin position="242"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="Acetophenone carboxylase-like C-terminal" evidence="5">
        <location>
            <begin position="546"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase B/oxoprolinase" evidence="3">
        <location>
            <begin position="737"/>
            <end position="1273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF19278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ80641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1275" mass="139011" checksum="091FC7CB9F549FAE" modified="2017-01-18" version="1">MAGIKIAIDRGGTFTDLIANPGTGKREDDVVLKLLSVDPKNYADANLEGIRRILELFQKKNIPRGSPLDLTGVDSIRMGTTLATNCALERNGEPCALLTTKGFEDVLXIGDQSRPQIFDLRIDRLDVLYSIVMEIDERVTMEDFAEDPDQLKTNLQENKDPNLVYGKSGEIIRIIKRPNEKEVLEVLQKIYDTGVRSLAVAFLHSYTFPEHEKLVGDLAEKVGFTHISLSSDISPMIKYVPRTHSSVADAYLTPVIKRYIDTFCMGVKNGTETKIQFMQSDGGLTDANKFSGLKSILSGPAGGVIGVAATSYHKDYPHAYIGLDLGGTSTDICRYSGVLEHVFETITAGITIQSPQLDVKTIAAGGSSMLFFENGLFRVGPDSATSNPGSACYRKGGPLTITDANLFLGRLVPEFFPKIFGPHENEPLDIDIVDKKFSELTETINKELGKNLTADEVAHGFLQVATESMARAIRTLTEAKGHIVADHRLVSLGGAAGQLISFVGESLDIDTILIHRYASILSAYGIFLADVVTEEQEPCSFILEESTAAIESKFQELTKKSLTSLLSEGFEEIKTEKFLNLRYVGTSNPIMVLEQPGVDFKTSFTNLHQKEFGFSYEEKDIIVDDIRVRSVGISHARETPSVDKQLSELEENIVDPKTSACFYKNVFFDGQRXNTPVFKIDDLPLGCTIFGPAILVDNTQTNVVPGSAKATVLEQHIVVQILKKSKNLLQDEDDAIDPVMLSIFGNKFMAIAEAMGNQLRKTAVSTNVKERLDFSCALFDSSGALLSNAPHIPVHLGSLSTCVTFQAKLWEGKLKPGDVIITNHPSCGGTHLPDVTLITPAFKENKVIFYVASRAHHADIGGITVSSMPSNSKYLFEEGAAIYSELIVKEGFFDEEKIRKLFYDIPASYPGCSGSRKLSDNISDLKAQIAANLKGIQLLYNLISTYGLKNVYKYMAGIMENAETTVKAMLNKFAHHYQQSSFYGADYMDDGAIINLHASLDVENEKYTFDFTGSSPQVYANLNAPIAITNSAIIYFLRCLVGENIPLNQGCLSAIKVIAPEGTILNPSNNVAVVGGNVCTSQRITDVLLKTFKVQADSQGCCNNFTFGIDTKLNPDGTLNKAGLGYYETIAGGAGAGADSWRGPGWNGASGVHTNMTNTRMTDPEIFEKRYPVLLKKFCIRKQSGGNGFYKGGDGVIRDIEFRSKLQASILSERRSLAPHGIDGGADGKRGLNLWIRKFDGAVINIGGKNTVDVSPGDRIVIETPGGGAVGKPKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R178</accession>
    <name>A0A1E5R178_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Acyl-CoA desaturase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g3957</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R178"/>
    <dbReference type="STRING" id="56408.A0A1E5R178"/>
    <dbReference type="InParanoid" id="A0A1E5R178"/>
    <dbReference type="OrthoDB" id="10260134at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004768">
        <property type="term" value="F:stearoyl-CoA 9-desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03505">
        <property type="entry name" value="Delta9-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015876">
        <property type="entry name" value="Acyl-CoA_DS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351">
        <property type="entry name" value="ACYL-COA DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351:SF31">
        <property type="entry name" value="DESATURASE 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="12">
        <location>
            <begin position="409"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ80651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="530" mass="61888" checksum="3468E57BFB2A90B1" modified="2017-01-18" version="1">MDSERNDIETLREAPAIPSFDYTGFLDDPEIKKFLDPAHVNKLKNSAKHDPYFIHLFKKINPSKAILNIVIPLYCLFILIFNKPPISTASNTDQIQPAKPLTANFYLQYTKSDIFLGVIVINYLLTLFAFFVGYHKYFTHHTFQTDSKALQLIFAIFGASVGVGSIWEFSSQHLLHHFHTDNEKDPFNAKHGWLFHMWGHNLFYGNKKAENAYFQMMKSSNDKRNLVFLHKKRKDQGNFNEFMIWQHRNYSYIVLLMIFVLPMIVSRLLHVNMWSCIFYLGLCKMSIIQQQWFLGETLGHHWKVNSCQSYDDSNSSMNCGFQWFWAFFTFGETQHNFHHEFPGDYRNSNKWYVLDFQKWIIYVLYQIGLVTRLHTTNSKQIRQAEIQQEQRNLDKLRKTLMWGVPIEKLPIITAQQFSAMAEQEYKVRKRALVCVEGIVHDVTPWVKDHPGGPSLIEMAIGKDASDAFNGGVYLHSKAARNLMANMRIAVVVSNKDGVWGKSSQDDNDQYRLRLERNRRRNKTYYAAGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1A2</accession>
    <name>A0A1E5R1A2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Plasma membrane ATPase</fullName>
            <ecNumber evidence="17">7.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AWRI3579_g4190</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H(+)-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>ATP + H2O + H(+)(in) = ADP + phosphate + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:20852"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Cell membrane</location>
            <topology evidence="2 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.1" evidence="17"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1A2"/>
    <dbReference type="FunCoup" id="A0A1E5R1A2">
        <property type="interactions" value="490"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1A2"/>
    <dbReference type="InParanoid" id="A0A1E5R1A2"/>
    <dbReference type="OrthoDB" id="116380at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008553">
        <property type="term" value="F:P-type proton-exporting transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02076">
        <property type="entry name" value="P-type_ATPase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000011">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000005">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000008">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006534">
        <property type="entry name" value="P-type_ATPase_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01647">
        <property type="entry name" value="ATPase-IIIA_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 17" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="14 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 17" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 17" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="312"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="683"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="710"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="746"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="785"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="816"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="849"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="19">
        <location>
            <begin position="60"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="23"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OEJ80657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="908" mass="99238" checksum="BD9B5B2B0BE45A02" modified="2017-01-18" version="1">MSDQVHEPVVEKPVEQKTPEKPVEDDDEDEDIDQLIEDLQSHHGGNDDDEEEEEEHHAAGAARVVPEEQLQTDPNYGLSSDEVSKRRKKWGLNQMAEENESLIVKFLGFFIGPIQFVMEAAAILAAGLEDWVDFGVICGLLMLNAAVGFIQEYQAGSIVDELKKTLANSAFVIRDGSLIEVPANEVVPGDILQLEDGTVIPADGRLVTEECFLQVDQSAITGESLAVDKHYGDATFSSSTVKRGEGFMIVTATGDNTFVGRAAALVNQASGGQGHFTEVLNGIGTILLVLVILTLLLVWCACFYRTDRIVKILRYTLAITIIGVPVGLPAVVTTTMAVGAAYLAKKKAIVQKLSAIESLAGVEILCSDKTGTLTKNKLSLHEPYTVEGVDDDDLMLTACLAASRKKKGLDAIDKAFLKSLISYPKAKALLTKYKVLDFHPFDPVSKKVTAVVESPEGEKIVCVKGAPLFVLKTVEEDHPLPEDIRENYENKVAELASRGFRSLGVARKRGEGHWEILGVMPCMDPPRDDTAQTVNEARHLGLRVKMLTGDAVGIAKETCRQLGLGTNIYDAERLGLGGMGGDMVGSELGDFVENADGFAEVFPQHKYNVVEILQNRGYLVAMTGDGVNDAPSLKKADTGIAVEGASDAARSAADIVFLAPGLSAIIDALKTSRQIFHRMYSYVVYRIALSLHLEIFLGLWIAILDHSLNIDLVVFIAIFADVATLAIAYDNAPYAAKPVKWNLPRLWGMSIILGVILAIGSWITLTTMFLPKGGIIQNFGSIDGVLFLEISLTENWLIFITRAVGPFWSSIPSWQLAGAVFCVDVIATMFTLFGWWSQNWTDIVTVVRVYIWSLGVFFIMGGAYYLMSESEAFDRMMNGKKKEKEGSRTLEDFLAAMNRVSTQHEKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1C5</accession>
    <name>A0A1E5R1C5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">U4/U6 small nuclear ribonucleoprotein PRP3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g4072</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1C5"/>
    <dbReference type="FunCoup" id="A0A1E5R1C5">
        <property type="interactions" value="769"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1C5"/>
    <dbReference type="InParanoid" id="A0A1E5R1C5"/>
    <dbReference type="OrthoDB" id="10264544at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd24162">
        <property type="entry name" value="Prp3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013881">
        <property type="entry name" value="Pre-mRNA_splic_Prp3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027104">
        <property type="entry name" value="Prp3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010541">
        <property type="entry name" value="Prp3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14212">
        <property type="entry name" value="U4/U6-ASSOCIATED RNA SPLICING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14212:SF0">
        <property type="entry name" value="U4_U6 SMALL NUCLEAR RIBONUCLEOPROTEIN PRP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08572">
        <property type="entry name" value="PRP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06544">
        <property type="entry name" value="Prp3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor 3" evidence="8">
        <location>
            <begin position="105"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Small nuclear ribonucleoprotein Prp3 C-terminal" evidence="7">
        <location>
            <begin position="369"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="38"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="201"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="112"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="253"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="56"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="492" mass="57371" checksum="F15AC4AF91F75378" modified="2017-01-18" version="1">MSTDKEHAASSRNTQSKGLNTEIHSALLNDNLTLIKTQFQQPKIPKGRGHKYNKNRNNDGQRNKRKWAEDVNNDSVIDNPYLSGGQIGALKKKPILDLNNFHTVPGGMSEKYEQERSRIEHERRAKAEAKLRREQEKQRDLAMVKAGEKPDATLNESLTLLTDSILEEFENNPWDLVFLNKTHTDIHDKYIKVDYRTLKAEDDSDEDVDESEDDDLDEDTESSTPSVRYVLHPVPVVKEHIQTIDGDVKPSKLYLTKKEMKKLRRNKRKLDRQAMREKIRLGIEPKPEPKVRMSNIMKVVQNNANISDPTLFEKQVKEQVRERAEQHDLTNKQRHEKAVENQTFNAHEANQKRKELVESGMKYEVHCLCFKISRLWNPKNRFKINTNCRQLKLQGCCLRLKGSGEAIIIAQGSEKSCKFFQKLIMNRIKWDESFVDKESGNEIDMSDTTVELKWSGLVGKSSFKGKWFMKECYDLQHLLRVLDEFDCRQYWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1H9</accession>
    <name>A0A1E5R1H9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aminopeptidase</fullName>
            <ecNumber evidence="11">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g3974</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9 11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the peptidase M1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="11"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1H9"/>
    <dbReference type="FunCoup" id="A0A1E5R1H9">
        <property type="interactions" value="1019"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1H9"/>
    <dbReference type="InParanoid" id="A0A1E5R1H9"/>
    <dbReference type="OrthoDB" id="10031169at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000001">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000002">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000002">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000004">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF174">
        <property type="entry name" value="PUROMYCIN-SENSITIVE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11 15" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 11" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="14">
        <location>
            <begin position="21"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="12">
        <location>
            <begin position="241"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="13">
        <location>
            <begin position="532"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="314"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ80732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="869" mass="98855" checksum="4D42ABE49D7A20D1" modified="2017-01-18" version="1">MCTCNRAAPPPEREILPTHLKPLHYDLTFHPDFETFKFKGKAVIDLKIQDSSVHYVELNTLELFDFEATVGKDHVKPLKIESIKETQRTRFHFPAELFTSTDDVLRLNVNYNGILNDNMAGFYRSKYKDALTGEEKYMVSTQFEATDARRAFPCFDEPNLKAKFTITLISDKKFVQLSNMDVASEVIENDLKTTKFNTTPLMSTYLVAFVIGEFVGVPSKYEYRIPVTTWTIPGTEKSAQYSADFTAKCLEFFEKTFDIKYPLPKMDSALIKEFSAGAMENYGLITYRDTALMLDLENSTLDEQIRVSEVVAHEVSHQWFGNLATMDWWSCLYLNESGATFWSWFCCNHFKPEWKVWEHFVTDSLQAALTLDGLRSSHQVEVDVNKADEINQIFDAISYSKGGSLLRMMANWLGEETFVQGVANYLREFSYKNAVTEDLWNHLGKQSGKPVPEIMSVWTKKVGYPIVTVEESGDKTLKLTQHRFLKTGDVKPEDDTTIYPIIPFLKTAADKIDKDTVLYERENTLEIPNSDFYKINGNQAGFFLTCYPESRYAKLGTQAHLLSVEDRAGLIGELESISAAGHTSTSNFLGLLRNWKSESSPAVWGQMLSSLAAVKTAWLFEDDKTTTALRKFTADLISDKLNELGWEIKESDDFELKSLKISLFSSSVNAKVDKYIQIGLDQFAKYAQGDSSAIDPLHKDIVFNAVAVDGKAENFDKLLAIYKKNDKDKINALKALSRFENDEIIPKIISFLLDESVIAPQDIYIPMANMRTSKKGVLALWDWIQNNWSEIEKRFPPSLNMLSYIVKISTSGFTSTDRIGEIEEFFSTKNCSGFDRALAQSIDSIKSKAQWVERDAPVVADFLKEHKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1J2</accession>
    <name>A0A1E5R1J2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Kinesin-like protein KIP3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g4334</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1J2">
        <property type="interactions" value="102"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1J2"/>
    <dbReference type="InParanoid" id="A0A1E5R1J2"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF13">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF19 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="8">
        <location>
            <begin position="229"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="99"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="215"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="901"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="100"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="920"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="944"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="966"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="978"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="301"/>
            <end position="308"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1005" mass="113436" checksum="4D31EE78482096DB" modified="2017-01-18" version="1">MAEENISRQSSIQVVVRVRPFTYRERLMLQDSSAQYSDSTVSRTSLLNDDLVLLNPNTSNENXELDPNASLLDHWNHKAEGVRRVVECVDDKMIILAPRNGSQSAGNHMSGKHTSNTRTKSNKDSGSLNNLKFIGLPNEPTLRNQPLNSSTSASHTRSITPRKRKLNISKKEAPQRSPHSNFRINSYSNNELPNGLENLSSSPTFLSRRKTSLIESRTTVSPTEGRKLAGGATRRVSSPSPGPMAALPSTRSTSAYKREHRYIFDRVFDESSTQEEVYEYTTKPLLNSIIQGYNCTIFAYGATGCGKTYTINGDPAAEIGSPERNNGLVYKVIQDLFVKLSEMQENENKEYELMVSYLEIYNETIKDLLSPQTPSKKLIIREDKEQKIQVANLSYHKPETLEDIMELIDFGNNYRTTNETNANKTSSRSHSVLQISVVIKNKDLNDDLSENQLSSVLSIIDLAGSERACKTQNRGARLQEGANINKSLLALGNCIKALCSXGNSGGGNNNVISATGGVTLEPTSLNSGAQDIYTPHVPYRDSKLTRLLKFSLGGNCKTVMIVCCSPSSEHYDETLNTLAYADRAKEIKTKLVKNSLNMNNHVGSYVKMIERQRLEIGKLKQYNELSLKKKREQINLKIREIIAGVETRLNLSTKFDNYKYTRSLILCKRRHLQILIMNFEHYMQFYSAAFQESSQGKDMSIPQFLQSVVGKLNEKIYLLETQYESEEALIDSFIDYENMISNLPLNIDSYNEVIDFPIVDSLIKALKDNVLNEILTNSIIMVENLIKDYSXIAMIPMSVVXPRLALDSAELLREFEQFVNNIDVNFEKFSAKMFENLFGDRAKLSTKNEHVFDQTNVLNNEALQKKKLRWTDTEAAEGLDLDIQDVKDXANEVVGDXQKEDIMMDQSDGNEDIVSSLDNHSGEESLSRDQSIDREYEIANNASNDDDDEEXXEEEEEELGNQSVVRNEDYRNEKSADNNDFNLTNQLGFQLKERPNSLVSRKLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1X0</accession>
    <name>A0A1E5R1X0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">RNA polymerase-associated protein RTF1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4371</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1X0"/>
    <dbReference type="FunCoup" id="A0A1E5R1X0">
        <property type="interactions" value="360"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1X0"/>
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    <sequence length="588" mass="69181" checksum="AC3C8A73A4C8C576" modified="2017-01-18" version="1">MSDLDDDLLALADGDDFSDQEDQVSVSRKRDATELSDSDDDESVPTKKRNKYTEYDEDMEEDEEDDYVPGGSYGNDDEDREEEEEEEEESMESAPYPLEGLYKNEDDREEILSKNEIEREQIVFQRSEEMKTYRERKMLRERAKQIRRDQQRIQSSSNRKGQKSTRSTEAVSAKDIRSSKLNELKKQRAKKRNQVEYGSEDEDNEYGSDDYTPAKTVKSRKGRYDDEEDEEDDYYEEDDEDQYYPGSGDRRGNTTEVEWAEDEYEADRDASIEDFNKLKLGRQFVDKFCFYPEFNKIVQGCYGRVNVGVDKHTGAPTYRMVKIEKVFLQKPYNFGRFFTNQYFGVTQGKDRKVFQMKFFSDGEITPNEYERYMSCLSNQDIQKPSLYDIKDKLSELKAFIEEPMNDKLMDQIVRNRLQFNKKLSGTNAVLEKTVLREKLQYAKDTGNERDVAKYSSQLRSLEKRLNMYEKHHENDQTGAKKLGALTNKNKRINIDKMREASHRIVQHDMKTFDMKSDPFSRLKTRTKVYYQEVQEAENAKAKEEARQKLLQENKDAAAHKEKTLLLAKFRRLGGLEELVPTIDIKFNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1Y0</accession>
    <name>A0A1E5R1Y0_9ASCO</name>
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        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">AWRI3579_g4170</name>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
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        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">AWRI3579</strain>
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    </reference>
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        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    <sequence length="506" mass="56469" checksum="5E077B308ACCE686" modified="2017-01-18" version="1">MNKVSVKSEAIDGQGKKDEQSGFIREENNFXSSKVKTLKPKIXSPHFGSSPNTSSISTVXSTKKRRKKTLFQQSKSSKSYQNSSVSGATSKNGFDPNLENKSLSSSLIXNNISANNTMGSKNHCDDSLMDVSSSKVKLEDSSRSXRKHDVAHVGNTETKFNLTSSFSHTDKENIEDISFSKMFSTPQFSKHSKVDFSQPVKQEKGLEHSQKKQXIKNTHXHQSXSLPEIDGTGKDVTHNEXXNCQSFDDAWLPSLQKFYLKEPEIGNMASNGKKTIPVAEDNSQSTVHKKALSFPSTNXEKSLKNIESDKSISQETSLILPEAKRFNKAHCSASTVVKKNVSFSLXNISIISGETSFEQKXPIRQEEPIPADCRDPGKYHAINELLYDRPTVTENEAFEDQSTRGKKSLSYIDLFQDVGDTQAQEVFASYGALSLEQWVQTGLDMNAKHQALLEKIIVSRLKLSYKFKVITDLLNEKAQELNQNADLINEKLNKIKXLGNEILNIM</sequence>
</entry>
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    <accession>A0A1E5R1Z0</accession>
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        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
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                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
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            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51263"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="OEJ80925.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="693" mass="76940" checksum="D01A40A80AAA3520" modified="2017-01-18" version="1">MALEPIDYRTHIRDIEQKYLKVINSEDPELKWLILSPNSSKEYEVAXTGAEFHDFLNSFQDTSVEFGLCKTSPPGSDVEKIVLIGWCPDSAPLKSRSSFASNFNDVSNNVLKSYHVQVTARDEDDLDENEILMRLSNAAGARYSIQDGSKKKFGSPVTRSTPARSTPSPHVKSTSSPSPASAESTPEPAPKPXPKPAPKPAPKSSPSPVAPTPVKASPIKTAPXKASPKPVAHDDWDEPEVEERDLSNAPLKPNTSSWKPIGKIDLQKIIAEERAKEDPRLXTKSTSSSSKINPADDIAALKEKSKLKRDQDYGSFLKKPESNAAFKEAPKKEEQSEKXNIPEAQKEDKQEEXDELKARFEKMNTDSSTSNEPPIIQPRKYNNHASSAGFNQSVDAPSPVLAGKNNYKKVGIPLPGMHFDEEKEEEEEKETSKPDNKSDDGWSDEEPQEAEDDDQRPIPSLPPXQRKESIPETQLSAQNSMTVETTEEEDENNEPPPAIPTREKTPAFSSRPGSPALFSRPNSPALPSRNESPKEVPLRQKLLSKEKEEXEKEEDDDEWDDDEKDEPPAPALPARGKSSASKLAKEVEPEEEEEEEEEKKEAPAPSLPPRHESPEPQLPPRRAAQPPPPPPPKKSAKPQAIAEYDYEADEDNELTFKEGDKIINIEFVDDDWWLGELESGARGLFPSNYVNQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R210</accession>
    <name>A0A1E5R210_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADPH-dependent methylglyoxal reductase GRE2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g4039</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R210"/>
    <dbReference type="FunCoup" id="A0A1E5R210">
        <property type="interactions" value="268"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R210"/>
    <dbReference type="InParanoid" id="A0A1E5R210"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000191">
        <property type="entry name" value="Methylglyoxal reductase (NADPH-dependent)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="6"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="345" mass="37758" checksum="0EC6A3A94736A1E6" modified="2017-01-18" version="1">MAKPTVFVSGATGFIAQHVVKGLLETKKYKVVGSVRNSEKATLMKAGFENNPDLSFVIVRDLAQIDAFDESFKQLGSEIKYVFHTASPVIFDITDFEKDLIVPATNGTKGIFQAAVKYAPNVKKFILTSSFSAVCSIAEENDSSLTLIEKSWNSQPLEEAMGNSVDAYGYSKTAAEKAAWDLHKKLDPKFSLTVINPAYVFGPQCFDSSVKEKLNASCQNINEIVHSNLGDPVKAQVKGSFIHISDLAKAQVSCIENHKLNGQRLILAKDRFSLQNVVDIINAKYPSLEGKITKGEPESAKQAIEGLAKVDNHVTKELLGFPLKTLTEAVQDTVDQILRVEAAQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2C0</accession>
    <name>A0A1E5R2C0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4079</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2C0">
        <property type="interactions" value="133"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2C0"/>
    <dbReference type="InParanoid" id="A0A1E5R2C0"/>
    <dbReference type="OrthoDB" id="3972310at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="378"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="406"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="493"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="606"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="633"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="669"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="671"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ81042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="701" mass="78361" checksum="5415B0A9885B3FCD" modified="2017-01-18" version="1">MAKLSSKLRTTGLGIGNGHRRISIGFLKPDKKNPLVKKYAVQNKRKRQRRGDEQRSVRSLVEDFGSSFRVSVYDEDEGDVIDYQDDQLEGDEGFGGQLSRSVSLPSRVSEESEVVGEPDVGWLLQEHERRYSQVPTIPEHDDEADNIEQDPRFYLQEEIHGHEVETXSTYDSFMRKLKMQSKSHLEEGGFPRRGSYGAISGRGEVPKIFEAEDDDEDFEHENVTFASESKILVRCSIPLIFTFLLEQIFSVVCSLVVGHLGKAELAAVSLASMTTNITLAVFEGISTSLDTLCPNAYGAGNYKGVGVHLQRCVALSMIVFIPFAFMWWYSEHLLLYVIPESEKELVVLTSKFLRVVILGAPPYILFENLKRFLQAQGIFDAAVYILLFCCPINILLSYLLVWNETIGMGFIGAPIAVVLNFWLMAIMLVLYTVFVDGRKCWGGFSKKALTHWKDLAKLAIPGVIMLEAEDLSYELLTLFSSYFGTEYLAAQSAIATTALLMYMVAFAVGVCSSTRIANFIGAKRVDNAHIASQVGICASLFVGLLNCGILNFGKNYIASIYTSDPQVFHLITDALPLVGFVEIWDAVNCIGGACLRGQGMQYIGSIVNLTVYYLLAIPLGMFLAWVLDMKIKGLWTGIGLGMFLIGAIESYYVLYPNWEDILERFEMMKETEDDSDDEEDDLAISDSDSDLEEDENTRLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2F4</accession>
    <name>A0A1E5R2F4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NADPH dehydrogenase 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4388</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2F4">
        <property type="interactions" value="869"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2F4"/>
    <dbReference type="InParanoid" id="A0A1E5R2F4"/>
    <dbReference type="OrthoDB" id="3969599at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02933">
        <property type="entry name" value="OYE_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000138">
        <property type="entry name" value="NADPH dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045247">
        <property type="entry name" value="Oye-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893">
        <property type="entry name" value="NADH OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893:SF91">
        <property type="entry name" value="NADPH DEHYDROGENASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="4">
        <location>
            <begin position="19"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ81086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="394" mass="44074" checksum="7F5D3A2410CD8CE8" modified="2017-01-18" version="1">MTITYDKRYTPVNLKDSNLFKSIKVGKLELPQRVVMAPITRMRAHPDQVPNVDGLAAEYYDQRSKRQGTLIITEATSISAQAGGYPNVPGIYTKAQADEWKKIFERIHNNKSFVFLQLWNIGRQASPAYLKAKNLKFIGPSDGLYTGKDSEKEAIGSGNELKSLTKEEILATVQDYVNSAKLAIEYGADGVEIHGANGYLLNQFLDPISNKRTDEYGGNIENRARFTLQVVDALVAXVGADKVGIRLSPFGQFGTMSGNDPLIVAQYAYLIGELEKRAKESPQKRLAFIDLVEPRVVDPMAEEGKHNDTGFSNEFVYSIWNGVIMRSGEYALNNKQALKDVNANDRTLLVYGRFFISNPDLIDRLEKGWPLNAYDRSTFYVPGAKGYTDYPTYX</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2G8</accession>
    <name>A0A1E5R2G8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cargo-transport protein YPP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4055</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in endocytosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic granule</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the YPP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2G8">
        <property type="interactions" value="263"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2G8"/>
    <dbReference type="InParanoid" id="A0A1E5R2G8"/>
    <dbReference type="OrthoDB" id="3972608at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23270">
        <property type="entry name" value="YPP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051722">
        <property type="entry name" value="Endocytosis_PI4K-reg_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23083:SF464">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT DOMAIN 7, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23083">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN, TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ81089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="783" mass="90233" checksum="7C242ACB81C4A6B2" modified="2017-01-18" version="1">MTQQTQAISQESSSAEFPLLRKDQIVDLLNNFFKVALVGSSASFSASKYANVEDQEAVDLLGAYNKIVKLHYRMNYNIFRVLAGETFKADRFEIILNDALKVKSSKLFDAASVKQDRAEYHQLLELTLTAVIGVCSYYLDDKDFAEKQLIQVLQSSNGTELESIASKYRKMLKLESYFFLNLLGKVMSQFTLSSILKQGIPQRTEAVSVFYLDLTIRQFTNYTTENNKFIKMYGDILCNRIQSAELLAMGELILSQASFPKADEANNFDLESFHYVLTFFYNFGLKDFSKWEAFMEKSISLTFQSIVITHSAALFYNKMAAKQTELKGSSTKLSLLHFENLLVYSGKYRQLHDNEHSDIVSSISLYEHMLEIFASQENINSKGIGQIFSASSSKNKQARNFVCLLKEFYQNNEIPLVSLDEAKYIDSNKLVNTLILPHNLTNTLSKSWLAIYSNFKDSLKTLLSGNLLFYLANSLQLHDSKDVTEKIDILYEFAYCLATQREAVMASKFLKSQILNRWPENSIKSWLLLCLLESRSENKSNAFKISSTVLESINDRLEQLTFEDKWYAIQTKLTQLSLIQEMFSVQDALELLPELFSLFQSLFEQEKNVEGYTIKRSKEYLLQSIWLFVANLYSRDANSFSDAXESVKESENVENVQYTNLNINIARGYLDQDPRSFERVIRHDPFNTDALVGLAQYTFETLIPDDTKPVFSPESNNTLSDLKLKLELCLENSIEGHFCPELWFYLSKIYDIYNDHIRMESALLNCIKYEEENPIRDFKHCRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2I3</accession>
    <name>A0A1E5R2I3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DDB1- and CUL4-associated factor 13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">WD repeat and SOF domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3927</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat DCAF13/WDSOF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2I3"/>
    <dbReference type="FunCoup" id="A0A1E5R2I3">
        <property type="interactions" value="1312"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2I3"/>
    <dbReference type="InParanoid" id="A0A1E5R2I3"/>
    <dbReference type="OrthoDB" id="10249065at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001105">
        <property type="entry name" value="WD40-repeat-containing domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007287">
        <property type="entry name" value="Sof1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051733">
        <property type="entry name" value="WD_repeat_DCAF13/WDSOF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22851:SF0">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22851">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA U3 SNORNA ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04158">
        <property type="entry name" value="Sof1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="106"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="298"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="341"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Sof1-like protein" evidence="11">
        <location>
            <begin position="376"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="436"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ81116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="472" mass="54591" checksum="9872798FACBF5223" modified="2017-01-18" version="1">MKIKTINRSADTYVPVKSTQESVLTRNLNPELHPFERAREYTKALQATKLERMFAQPFIGQLGDGHRDGVYHLAKNYHILNQMTSGDGEGIIKFWNMGDRSEKLSYKAHYGLVTGLSMTPMKLGYDAGMLLSCGDDKTIKLWDVGSLNNELIGKHQDQDPSQSVNKTGLIKTFHGETAFQGLDCHRNKASFVTGGPSVQLWDTARSRPLTDLSWGCETVTHVKFNQVETDILCSTGTDNSVVLYDLRTNSPTQKIVQSMRTNSICWNPMEAFNFAVANENHNVYYYDMRKMDKALQVFQDHVSAVLDVDFSPTGQEIVTGSYDKTIRVYNIHHGHSREVYHTKRMQHVFQVKYTMDSKYIVSGSDDGNVRLWRNVAWERSNVKTTKEKTKLEYDEKLKEKFKYMPEIKRISRHRHVPKVVKKAQEIKKIELDSIKRRENNERRHNKNMEYVSERQKQIKGKAFQYDEDDQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2M2</accession>
    <name>A0A1E5R2M2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ribosome quality control complex subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2M2">
        <property type="interactions" value="60"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2M2"/>
    <dbReference type="InParanoid" id="A0A1E5R2M2"/>
    <dbReference type="OrthoDB" id="205993at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006994">
        <property type="entry name" value="TCF25/Rqc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22684">
        <property type="entry name" value="NULP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22684:SF0">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT TCF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04910">
        <property type="entry name" value="Tcf25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="706" mass="81493" checksum="8690BA1AA6F700CC" modified="2017-01-18" version="1">MSSRALRKLQKKNNLDELSLLEDKLNAKSLTDTTADSKKGKVSHKQPANLFSMFGDEKSESESSEEEEKGEDEEEQYGKQSDDGNXKSQAALNSSFSNVTSKTAKTSKNKNSKSNDAESEDDFDKLVDQFQKENMQKYYAGINQEGEGTLDVADSNVYSIKNDPGFTKFRKHSDIQALFSSIKTSFLDPQTELKSLFDDIPLSVMNETSDSSGLSQHQQQQLKRMKRLVKNWATGKNKRTVPNGPSLTKTLLFSNIRDDWIPTQRGELHMKVLNDQDFLDWNAYLRPNDWLDVIKEDISVQQKNXPKIKYFKFEPLSREQTSKNMVEFYLSVVLHPDHESLISLIQSQAPYFVPGLLQVATILVRQGDRSNSNGLVEKALFVFDRALKNGLTFNGIDXQLPYIFFFNRQFYLAIFQYIGILEQRGCIGSASNWCKVLWSLSPCEDPLGCRFFIDHYLLQAQDYKFLVDLSRNKLFTQYKEWQTFNLDISIVLAFXKMDLHEQAAGHMKAAFQRHPYAVKEFYLQGLHGNPXXLPKNIQKLQLSSVAEDIQLSFYLLRMSKYWSDLDITKMTEILDEIWRAGVXNETFENTLDSKDAIQNERLYLEGLEINLLRFTLLSQESSIMHKLPTKIFEKYQIFDYDVLPPPFNESLEKIDALPLETIHDFINKKDLEEKSSHLLQDQNLLDLIQRVSLQDYIQDNTEQPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4M0</accession>
    <name>A0A1E5R4M0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">DNA-directed RNA polymerase III subunit RPC3</fullName>
            <shortName evidence="9">RNA polymerase III subunit C3</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g3498</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4M0">
        <property type="interactions" value="515"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4M0"/>
    <dbReference type="InParanoid" id="A0A1E5R4M0"/>
    <dbReference type="OrthoDB" id="272392at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055207">
        <property type="entry name" value="POLR3C_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013197">
        <property type="entry name" value="RNA_pol_III_RPC82-rel_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008806">
        <property type="entry name" value="RNA_pol_III_Rpc82_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039748">
        <property type="entry name" value="RPC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12949:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12949">
        <property type="entry name" value="RNA POLYMERASE III DNA DIRECTED -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08221">
        <property type="entry name" value="HTH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05645">
        <property type="entry name" value="RNA_pol_Rpc82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20912">
        <property type="entry name" value="RPC3_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22536">
        <property type="entry name" value="WHD_POLR3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 14" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase III subunit RPC82-related helix-turn-helix" evidence="12">
        <location>
            <begin position="17"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase III Rpc82 C -terminal" evidence="11">
        <location>
            <begin position="169"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase III subunit RPC3 winged-helix" evidence="13">
        <location>
            <begin position="492"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="366"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="403"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF22536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ81834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="652" mass="74560" checksum="4E2214CF66F814B0" modified="2017-01-18" version="1">MNVGQTLELRTIYSESFLFTNLVQAYFGERASEVINVLISKGRLNCREISRLTKIDYKTVKKALISLIQLKCVSYFEDKDALNGSSSSNQGNVYYSYNEEGIITLLYSGEIISQVTKLYSNHHLLSKNNSYKLIGEILQNVLQYGSLNPSDFIASLTNDNTSSQIYQTIFIQLVEDNFLVPIKPIDYCPIQDLWHLLYTKEYKAIPKSSTLSDLKKREQAKGKAKETFIKLTDKSYSSSSNVITTDKKTLLRKISDNVYLTFNLQRYFKHKRSNHLVQLCASTVGKIPSKILECALRYTERSAPNVCNPLSKTGLLQDLSEQEALKDELTLQEEKNNGVTFNAIDISKILDLNVVLQDSLXSKLKDKFGKNKNNKRKQANSSXSNKKIKLENGATLNDIPEAANEDENDDENEAAENYNDDENDNDDDNDDILEAQLDSESSSHSITLINAHLKLLSDPNAQVQFLRESRAGLYYVPYSSLTPLLKSYTYDALVESTLGLSALRLLRSIRINRLVTEKTLNNLVLFNEKDVRSILSKLLKCNSVEIQEMPRTMDRAASRSVFLFRNNQQHALQFMEKNLCWNIANLIYKTELLKKENQTLLEKAEREDVKGREEELLLSSELNQLKMVNERELNSLTRMNRLISLWEVFKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4M7</accession>
    <name>A0A1E5R4M7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Biotin--protein ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3491</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4M7">
        <property type="interactions" value="130"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4M7"/>
    <dbReference type="InParanoid" id="A0A1E5R4M7"/>
    <dbReference type="OrthoDB" id="10250105at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin--[biotin carboxyl-carrier protein] ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03144">
        <property type="entry name" value="GATase1_ScBLP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019197">
        <property type="entry name" value="Biotin-prot_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835">
        <property type="entry name" value="BIOTIN PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835:SF5">
        <property type="entry name" value="BIOTIN--PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09825">
        <property type="entry name" value="BPL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="1">
        <location>
            <begin position="367"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="688" mass="77013" checksum="4965542BE130FDA7" modified="2017-01-18" version="1">MNVLVYNGPGASPVCVHHTVETLRNLLEPYYAVTTISNRALLTEPWMTKTSALVFPGGADIPYTKECQPILSEIQKFVKKKGGLFIGICAGAYWGTSRCEFYENHPTMEVSGNRDLKFYPGIARGPTFPDFHYKSQSGARVVTLNVDNGTKVKSYYNGGCSFISPEAYENVEVLARYTDEKDEMWVQLKSENEKSKDAAVVLCXQGKGKALLIGPHPENVPEIMLKSKEDGPYDTNLLKELILDNQNRKTFLRYILAKAGLKVNDSDTYSSAVPLTPIYLSVDKKPSPRLKELKTHLTPDLVDVDHYHLKSNVDEFDIYEGFMNPKVKSFTSSPYDRTYSDPKVIVFPSDNESCVPTAKTPKFDAEKYFANKWPTTEYGSMLLYGEVVTSTSAILDNNKSLLSMFPQNSVVFLGTHQVMGRGRGGNIWVGPEGTMATTICTSFPLTSPVTGKPLSIVFVQYLSSLALCKTIKTYAKGYENIPVKIKWPNDLYIMDPQYYYNNKLDIFNMKPVPLNDIEPSYVKSSGTLINTNFMNGSYTVLVGTGLNATNIAPSVSVQRWVDIINEELRKRSDFDEKDLLPDVEHEKLLALYVNHLNETINSFINVGPKGVLGEYYENWLHSSQIITLTEHNSVRAKIVGITEDYGLLIAKELMVGSDDVFTGTVHHLQPDGNTFDIFKGLISKKVYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4N2</accession>
    <name>A0A1E5R4N2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">LETM1 domain-containing protein YLH47, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3658</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4N2">
        <property type="interactions" value="556"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4N2"/>
    <dbReference type="InParanoid" id="A0A1E5R4N2"/>
    <dbReference type="OrthoDB" id="275278at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033122">
        <property type="entry name" value="LETM1-like_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044202">
        <property type="entry name" value="LETM1/MDM38-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009:SF11">
        <property type="entry name" value="LETM1 DOMAIN-CONTAINING PROTEIN YLH47, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009">
        <property type="entry name" value="LEUCINE ZIPPER-EF-HAND CONTAINING TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07766">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51758">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="120"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Letm1 RBD" evidence="10">
        <location>
            <begin position="166"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="415"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="415"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="11">
        <location>
            <position position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ81877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="441" mass="50097" checksum="9339519FBA56A858" modified="2017-01-18" version="1">MMRSYATAVAVNRNLILRNTNRLVLPSVYTRFNSTSSTPPKPNAAPAAKDTKXAVVATNAPANKPTLWTKVKHEVQHYWDGTKLLGLEMKISSKLLLKMSLGYELTRREMLQLKRTTTDVVRLVPFSAFVIIPFAELLLPIALKIFPNLLPSTYESSVDKEKKLSSLRKTRTLMTEIMKDQKAAKHFKPSNINEAQKKLFNQFYQHVRETGEPESRGQMIKVAQLFTDDTVLDNLTRSHLIAIAKYINLQPFGTDVMLRYRIRYKMLQLKKDDLQIHYEGISSLNPAELRMACANRGIKIVNVDDLVLRDNLRVWLDMRLKDKIPSTLLLMATAYNYGELVGKNTLYDSLCDVLSGIPDELYHEVKVNVLEGADSVAAKSKMNYLKEQEEIMKEEVEQEKGTVIRVRDNINLDSVNNVSPDQLSSDAQSPVSNEVKADKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4P0</accession>
    <name>A0A1E5R4P0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Prefoldin subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3811</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4P0"/>
    <dbReference type="FunCoup" id="A0A1E5R4P0">
        <property type="interactions" value="985"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4P0"/>
    <dbReference type="InParanoid" id="A0A1E5R4P0"/>
    <dbReference type="OrthoDB" id="6375174at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23156">
        <property type="entry name" value="Prefoldin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000001">
        <property type="entry name" value="Prefoldin subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016655">
        <property type="entry name" value="PFD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12409">
        <property type="entry name" value="PREFOLDIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12409:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016396">
        <property type="entry name" value="Prefoldin_subunit_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="80"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="144"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="87"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR016396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ81879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="199" mass="23373" checksum="ACD40620FD53D9EE" modified="2017-01-18" version="1">MENLLRPKKTNPRGIPEAPFIEKVEEYVVSPDQFEQVFQAFQERLQQYKYMMESKNKTFSDLKRRLPGLESNLKMCGVLKGGKSAGDEDEDEEEDEEEENKQIEVNYQLDDTLYTKAVIEKPVDKVALWLGADIMMEYPLDEAIELLHTQIKDCEEKLIEAEQDVEFLRENITTMEVNTARLYNWDVERRQKERLAASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4Q1</accession>
    <name>A0A1E5R4Q1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Vacuolar protein sorting-associated protein 52</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3794</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS52 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4Q1"/>
    <dbReference type="FunCoup" id="A0A1E5R4Q1">
        <property type="interactions" value="887"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4Q1"/>
    <dbReference type="InParanoid" id="A0A1E5R4Q1"/>
    <dbReference type="OrthoDB" id="19482at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007258">
        <property type="entry name" value="Vps52"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048361">
        <property type="entry name" value="Vps52_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048319">
        <property type="entry name" value="Vps52_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14190">
        <property type="entry name" value="SUPPRESSOR OF ACTIN MUTATIONS 2/VACUOLAR PROTEIN SORTING 52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14190:SF7">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 52 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20655">
        <property type="entry name" value="Vps52_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04129">
        <property type="entry name" value="Vps52_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vps52 coiled-coil" evidence="6">
        <location>
            <begin position="45"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Vps52 C-terminal" evidence="7">
        <location>
            <begin position="217"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ81860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="555" mass="63758" checksum="C69E3A05F089DE28" modified="2017-01-18" version="1">MDVLVQKFESLDQEWSDAPLSDNGTQPLTKELDNTNQDKFKAELDSLAQTDKIIEAILSHTDAYSAGSLDFTRQIDTLSLELNRIKGKSVELENVSSENQSKLSELDQKIHQYKLLSPSLVERIIHGAIDARWCEDAELVVSYSQESAAEETDLCTILKHVVLERCKRKLVEAIKKLRTFYPSQVVQKELLNVPTVFEIIKSENKELAEQLFQAYCNTMQWYYTQFFARYIRSLTIMQYHQIDANYKFGGQLSYYYSMISGVVGVLSVNDYFKSVNKRIFILSQENSTVMVSQIAETNTMKNFLEIGFKNLNLCILNNYRVESEFITNFFQQKPQPSEDAASNTTKSKTTNDHLQVIFSKVFANAETFTKNMIAYTFDFYGLLLCIRISQIYIDKHASMNSASESAFESASESASTDPFEEYLNSQLFQILWPKFQSIINNNLNNVTDISNVDPDQFISFLQGILIISTNYNIRVEPLYQSLMNILNNFETYNLQKTHAGNKTYQLNVYNKLVNLLDSMESEQAQDSEVSSPKDDETRSIVVELKERYINCIKNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4S4</accession>
    <name>A0A1E5R4S4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Retrograde regulation protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3806</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4S4"/>
    <dbReference type="FunCoup" id="A0A1E5R4S4">
        <property type="interactions" value="320"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4S4"/>
    <dbReference type="InParanoid" id="A0A1E5R4S4"/>
    <dbReference type="OrthoDB" id="690068at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="152" mass="16699" checksum="14E884D10737CCEA" modified="2017-01-18" version="1">MSDKRIETKSLADINEKIQEIYTYLPKELFRDDKAPRTKDGKPNKGMILTAAVSHLLDLQSQVDVFNREEIELMVRLEELQKQEKQKFGKNIVDTSQINFENTSAEIALGAIGVGPLAGQLAASPGPSPFNNTSNEAVGGIDPLSLPSETFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4S6</accession>
    <name>A0A1E5R4S6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Srp40 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3577</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4S6"/>
    <dbReference type="InParanoid" id="A0A1E5R4S6"/>
    <dbReference type="OrthoDB" id="5599646at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007718">
        <property type="entry name" value="Srp40_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05022">
        <property type="entry name" value="SRP40_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Srp40 C-terminal" evidence="2">
        <location>
            <begin position="247"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="118"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ81868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="319" mass="32749" checksum="519CE30389531996" modified="2017-01-18" version="1">MGPAPKLSEKAKKKEEDDSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSDSDSDSSSSSSSSSSSSSSSSSSSDSEPETKKVMKKVQESPKKLVKKXLDSSSDSSSSSSSDSSDSSSSSSSDSSDSSSSSSSDSSDSSSDSSSSSSSDSSDSSSXSSSSSDSDSSSDSSSSSDSEEESTSNKRKLEKTVEVSNKKVKEDSXPKENSTESEKLSISAGVDEIKEGQRRHFSRVDRSKLSFEAWDLTDNTYKGAAGTWGEMANEKLGRVRGKDFTKNKNKMKKGSYRGGTITLESGSYKFKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4Y4</accession>
    <name>A0A1E5R4Y4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 17</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 8">Mediator complex subunit 17</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MED17</name>
        <name evidence="10" type="ORF">AWRI3579_g3538</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4Y4">
        <property type="interactions" value="184"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4Y4"/>
    <dbReference type="InParanoid" id="A0A1E5R4Y4"/>
    <dbReference type="OrthoDB" id="5319830at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019313">
        <property type="entry name" value="Mediator_Med17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13114">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13114:SF7">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10156">
        <property type="entry name" value="Med17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="25"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="136"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ81962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="673" mass="76279" checksum="C88D5373BE9E3BD9" modified="2017-01-18" version="1">MSSHEPNDGNIFLSLDPNLIYVTEAFPTPGSQTSLKREERDDDDDDDGEKDRTLADSEEASNLQEKANFQVLKHLKKSLVNNPYESYGSLPLNEFIPLILRQRGIQFSQLTSEYLQSELSEMAKQDAQSEIVDGTDSKQANENGHVVSPMTNSSPETSEPLEIDESHFMTPEEFNKCKQEIFXSVSMALNESSLSLELVSLLLSAVRNRQGMATMSPFLKNTVPPGSLCADKIEVSKLTQNQAAEKYDITIGMKLQSMRSAQEMISANYKSLTEAFKKEQQFWTTISDNFSNSNAIFKXRDRVSGEKSLGVQYGYGDSGSTFTGDKGIAILKAVHDSSERKEYLEMAPMSKDLGKTVLPTDETNKPLGFYTKLRIYSQLEDESDYILTGESSLHKDVAKMFKDAETENNLKAQTDCLKFTIFEQELMYQMRKETNELFKYGCTIQNENKIVCEMAQEKFEIELLPVDSDLLETYDQIPAKTDDRRANIFLVILRMLLVVMFKKKLLHDRTHQPLYSRESTSNKAVVFAGTKSINKYKSELLLIKPLLGKLRHSQYAILLKKIARDCILNVVKGSRVINDSPSTETSVDRYPASSDSNIELLQEQIRLFDFILKVPESVYEVELPSKDTYKLTLQSPNYCGVLIKLSISGKDSLEFTEFKELEEYLNYSISISQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R547</accession>
    <name>A0A1E5R547_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SURF1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3655</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Probably involved in the biogenesis of the COX complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Mitochondrion inner membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SURF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R547"/>
    <dbReference type="FunCoup" id="A0A1E5R547">
        <property type="interactions" value="367"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R547"/>
    <dbReference type="InParanoid" id="A0A1E5R547"/>
    <dbReference type="OrthoDB" id="10040024at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06662">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002994">
        <property type="entry name" value="Surf1/Shy1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045214">
        <property type="entry name" value="Surf1/Surf4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23427">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23427:SF2">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02104">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50895">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="389"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="43"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ82030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="436" mass="50440" checksum="175F9693F8F29EC0" modified="2017-01-18" version="1">MFRVRPAQIQLLCCGSRRAPTIPHFIAQRTVKTSTVDWKPIKSSRSPNEEQNNNNGSKRGNFAKYIFLSLMIAMPIVSFKLGMWQYRRLSWKNKLVATCEDRLISEPLNNQTGVFKMLFKNTNLDKIAECDLEKFREFTDDVNQVVDEQFQYKRINLTGQFDHEHELFVGPRMRDGQKGYLLFTPFVLQSGKGPNDLDARKFDGKKVLIERGWISADKVLPMERALTHLSLPQGIVQLECVIKVSRNLSATQWERDNKDGRLWQVIDFPEMCKEVGVAMPILFQQMYDLKDHKYEVIYEDDKSSKEKYQKGSSSSSWFSKLLGRQLEPPATEPAPVDASQKFTIKDTVIHATDNSGTEEEEPEYTKYQFVTAGVPLGKNPKIEYKNDHLQYMVTWFGLSFLSSIFLIVALRKFSKPNVISQEQLKRDKLRHASRNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5I1</accession>
    <name>A0A1E5R5I1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Mitochondrial metalloendopeptidase OMA1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3588</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R5I1"/>
    <dbReference type="FunCoup" id="A0A1E5R5I1">
        <property type="interactions" value="226"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5I1"/>
    <dbReference type="InParanoid" id="A0A1E5R5I1"/>
    <dbReference type="OrthoDB" id="7464992at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07331">
        <property type="entry name" value="M48C_Oma1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051156">
        <property type="entry name" value="Mito/Outer_Membr_Metalloprot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22726">
        <property type="entry name" value="METALLOENDOPEPTIDASE OMA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22726:SF1">
        <property type="entry name" value="METALLOENDOPEPTIDASE OMA1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M48" evidence="7">
        <location>
            <begin position="130"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ82128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="325" mass="36976" checksum="E7818F3C038C4851" modified="2017-01-18" version="1">MFKVFQRAYHRRVVYKSWNGATTGSGSANVINMLSTPRGKRNLALFVGGSTVFYLTNLHEAPVSKRTRFLWIPRSWELKIGDYSYNSMMSQVQNKILPTSHPEHKRVARIFHRLVEAAQNDAQDPEVREQLKDLNWQIHVINDPHQPPNAFVMPGGKVFVFTSILPICANDDGLATVLAHEFSHQLARHSAENLSKSPFYSILGLTLYGLTGMSVFNRLLMDACLRMPASRQMETEADYIGLMIMSRACYNPNESIKLWQRMANYEKSVSGYAGNVEFLSTHPSSANRIENMRQWMPQADAIYEQSDCGMFKSFNTIPKPFGLPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R6I3</accession>
    <name>A0A1E5R6I3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">Polyadenylation factor subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3828</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for 3'-end cleavage and polyadenylation of pre-mRNAs. Also involved in chromosome segregation where it has a role in chromosome attachment to the mitotic spindle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R6I3"/>
    <dbReference type="FunCoup" id="A0A1E5R6I3">
        <property type="interactions" value="253"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R6I3"/>
    <dbReference type="InParanoid" id="A0A1E5R6I3"/>
    <dbReference type="OrthoDB" id="16717at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045245">
        <property type="entry name" value="Pfs2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22836:SF0">
        <property type="entry name" value="PRE-MRNA 3' END PROCESSING PROTEIN WDR33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22836">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="157"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="227"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="262"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="394"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="473"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="476"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ82153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="515" mass="58232" checksum="4333305106916644" modified="2017-01-18" version="1">MNRTFGSGSNMAPLGNPSGMNNFNDNLDGNSSLGAVKKNPAPRRVVDLNNGYNRYLFYLKNRIVKNNFRHIQRNSGFTSELLPPTVINQMNMTGVLPSQATEINTMTKLQHLSNKKHVNSINTVKFTPTDGRRLLVAKSSGEFMLFNADDYSFETIMQAHDAGITACEYSNSGDWLVSGDMDGIIKIWQQPSLNLVKQIDPRNAVPSGPGGSSLVSNRYDELVVGSIRDISFNWDDSRFISCGDDKLVKIWNFQTGQLETTLKGHHWDVRTCDWHKEYGLVVSGSKDNLIKLWDPRSGKVVSTLLGYKHTVLKTKFTDPSTNKLISVGKDKTCRIYDLRNNCREYKTYKEDLNYISLELHPVQKTTFIMGTYDGQIKFYDFSKETLNESYHTIPHAHEQNINTLSVNPRGHMLASGSRDRSIRFWTRGRPYDPNAFDEPVYNNEKINGWYYGINNGPNAVRQKTDHGVAVPENDDLQGTTTNFMPNVTKDKLSSTNKHSKKTDDLEDEYVLPGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7Q8</accession>
    <name>A0A1E5R7Q8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Regulatory protein ADR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3184</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7Q8"/>
    <dbReference type="InParanoid" id="A0A1E5R7Q8"/>
    <dbReference type="OrthoDB" id="10018191at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000012">
        <property type="entry name" value="RB-associated KRAB zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051061">
        <property type="entry name" value="Zinc_finger_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179:SF13">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="84"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="112"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="474"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="485"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="12">
        <location>
            <position position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ82934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="628" mass="71953" checksum="17A3C11E801E068F" modified="2017-01-18" version="1">MKVNSPQEIDFNMDDTYSAHNEHDAVXESDDGNXSLAANSTFKIQSKNKKKQKLPQKQKPSKNGKYAQTSSHERVLKKVLDRNFPCGKCGKAFTRMEHLTRHERIHLNLKPYKCGNCMMEFSRKDLLKRHADMDHDGDIGDDAMFKDDKKQYNLKKYQRIYCNKESNIKRMEKKKQQKKMPKKKFDKQVLDDAADLNNLENIENLENIRSNIKFDGKKHQPHDKNATCTPKKNLQIQHNTKKFNSNNEPGEMVKYMQSDELKTLIKECNKFEPHDKVSKFPCEVVLPLAPVITKLEVPSITFNMCPNGDPTNCQSPNSFLIQDTSRDFHFHEDINSSFNLENQKPCNERIDNNYNIPRGCSVPLEXKNIXIPFAKKIIEPLIYTKNKNTLIEKNSVNLKENSSSATNLMDCNYIYDGAVQYCYGSRTNSNKKHFASYEEKIEFPGMNENFESLSFTNKENADYIERSKKKRKSKCESAGNCGNKDDDDXDDDDDNDNNDNNNNDDDNDDDGIYSPDNKLDCTSVFIDSSEIILDVVIDYDTPENENFQTGSTSNTKIDXPNGSTFQTCSSSFSSRGLSNQENETFFNNTEHTFSNLVSLCDAPTHXFQNGNSNANFLVKVCLKSQTDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7S9</accession>
    <name>A0A1E5R7S9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable vacuolar protein sorting-associated protein 16 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3246</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Essential for vacuolar protein sorting. Required for vacuole biogenesis, stability and to maintain vacuole morphology.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the VPS16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7S9">
        <property type="interactions" value="1085"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7S9"/>
    <dbReference type="InParanoid" id="A0A1E5R7S9"/>
    <dbReference type="OrthoDB" id="1792at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.780">
        <property type="entry name" value="Vps16, C-terminal region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016534">
        <property type="entry name" value="VPS16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006925">
        <property type="entry name" value="Vps16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038132">
        <property type="entry name" value="Vps16_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006926">
        <property type="entry name" value="Vps16_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12811">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12811:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04840">
        <property type="entry name" value="Vps16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04841">
        <property type="entry name" value="Vps16_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007949">
        <property type="entry name" value="VPS16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vps16 N-terminal" evidence="4">
        <location>
            <begin position="130"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Vps16 C-terminal" evidence="3">
        <location>
            <begin position="525"/>
            <end position="832"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR007949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ82970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="841" mass="97705" checksum="C8E28E7C22143F59" modified="2017-01-18" version="1">MKTPSFGWERLEKSFYRNRIIAQPSVYSQEYTEETIFDINYDLIALYLPDAQRIECYNSKKELMFSFATPNLKHLIKIEFTMDSFYLITRTNIRVXTSFQPLSFKDYDFCTSQLQTTSRGTREIGPSNEEEQHSLEESXEEIWSYKNTYVLSNKSQSIYKFNGSGFIKVLANNDQYTLLTEQHWDAQNNYLVFLSTNSLLYFDIKSGKLELIESDILWQKCQIAPSSPFVFLYNENSNKLSIFSLSAKKEIVAIKLSFIPSDIKWCGNDAVGCLNNEDDEVNXYGPSGKYITFWYPXNENSGKLLMQTTYFGLKIYSTETVSLISKVEESSFNIFRMGSTSPSAILADCLQLLNAGFAPRAIENLKSINLLECASECIEAALQEFDSTWQKQLLTAAAFGKASILKANDDEEVLNLSRKFVYACNTIRLLNNLRNITSYRFTFKEFQYLGFDKILHHLIQCNNKFSECLKLCELFHEKKHDYKMKVMYYYAVRKILVSNEETDEQLYREILAKFVKLHMDKSFDFSKLSNVCYQEGRLQLAKQMILADSNVENQLIPLLNLEEDELPLQKAHQSGSNLLELYVLLKLKNRLTNAQLFRLMALNLKVNNNLLVEYYRDSTKFGMSFDFYKQTDDYKNLSNLIMEKNVAQKQIVLEQVNELYKKDVGLNGDFRQCSSRELDLILFQKDLAMKMEHQDLGSMTCNQTLKILIQSSQSKLITKFLKKFSISDKVFYAIKSDVLISKKNFESLWDMCNERRFANNLKQLFKKLKKAGHPEEASRYVVLMKNETYETKLNLYLECKAYNSAIHLAIQQSDHDALVQISNMITDKNMKKAIQEQLAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7T4</accession>
    <name>A0A1E5R7T4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3402</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R7T4"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.100">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="84"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="173" mass="19089" checksum="AAE1B28538A84799" modified="2017-01-18" version="1">MQENKTRTFTIPCEDLLHVLVTEPISQDDFKTPITITVSATPISNSDSIKHASEXLSCIYYVIPNYKKSAARGTVSASTPLAHSSSSSSSSSSTSSSSSSSLANKYFLTTLQECTDNNDLKEFGKKMCLLNYQLYGLKNPVYVIVSSTLEITSSLDMMMMLNAYKQNRQDLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7V8</accession>
    <name>A0A1E5R7V8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Nucleolar protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3244</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7V8">
        <property type="interactions" value="1274"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7V8"/>
    <dbReference type="InParanoid" id="A0A1E5R7V8"/>
    <dbReference type="OrthoDB" id="267048at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12676">
        <property type="entry name" value="RRM3_Nop4p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12677">
        <property type="entry name" value="RRM4_Nop4p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000680">
        <property type="entry name" value="NOP4p Nucleolar protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034809">
        <property type="entry name" value="Nop4_RRM4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034808">
        <property type="entry name" value="Nop4p_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051945">
        <property type="entry name" value="RRM_MRD1_RNA_proc_ribogen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48039">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN 14B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48039:SF5">
        <property type="entry name" value="RNA-BINDING PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="12"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="139"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="287"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="459"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="88"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="221"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="624"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="262"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="647"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="659"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="673"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ82968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="683" mass="77867" checksum="B3275785D00DF0E4" modified="2017-01-18" version="1">MTSVQEDNLDNKTLFVRSVPFDASDEEFTNFFAQFAPVRHAVIVKDGEQKSRGFGFVNFADEDDAKQALGKARRTKFKNKLIRIDIAKRRERKGNKRSTSEETSIPDDEAAVSLGKESVIDDSTGEKNEEDDGEFKGQPKLIIRNMPWSCRDPTKLTKIFSRYGTVDNAYIPRKAGGRMSGFAFVVMKKISACRRAIEESKSLKIDGRQVAVDFAVSKNRWDEHKDESDVEESIADENKSDEKSVINEENAKTDEPNSDQEDVXKDEQEKEEEKVXERPKKNRKENFSVFVRNVPYDATEESLEYHFGKFGSVKFALPVTDKETGLPKGTAFVAFHNEESYQKCISDAPDVNTTSLLISDDVKPEYVYEGRVLSITPTLDRENANKAAEKKSLQRKEALGKAPADKDKRNLFLLNEGRINEQSKLAALLTKQDLEVREKSYKQRVDQLKSNPSLHLSLTRLAIRNLPRSMTEKSLKALGRKAVVEFATEVKEGKRHALNKEELDRSTKAKYKGMSEEEIELEKNKKKKHGVIKQAKIIMEVKGSTVGRSRGYGFIEFRDHKTALMGLRWLNAHEVSKEEVLEGLTDEEKKGIDHESFNKRRLVVEFAVENTTVLKRRQERVYTSRKRSAFGDSETRPDRSFKKQKTTESLSSSTLQPENKNKSGMSDDVKRLIGIKRKRRSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7W0</accession>
    <name>A0A1E5R7W0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glycolipid 2-alpha-mannosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3256</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7W0"/>
    <dbReference type="FunCoup" id="A0A1E5R7W0">
        <property type="interactions" value="118"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7W0"/>
    <dbReference type="InParanoid" id="A0A1E5R7W0"/>
    <dbReference type="OrthoDB" id="3970169at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000051">
        <property type="entry name" value="Alpha-1,2-mannosyltransferase (Ktr4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF8">
        <property type="entry name" value="GLYCOLIPID 2-ALPHA-MANNOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018153">
        <property type="entry name" value="Glyco_trans_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR018153-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ82987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="412" mass="48350" checksum="59EAB048FE3BD3A4" modified="2017-01-18" version="1">MAVIVSKKIVRIVLVALVLITGLLYSTRHISSDLNSEQLMQKLNENKDYLANSVGLKTSSNDTEEDEDDEDAVKKPFNVDKMFKPPGTPVKACFVSLVRNSDVWSLMPSIRSVEDRFNKNHGYTWVFLNNEPFDTTFKKAVSNIVSGEVKFGLIPEEHWSYPDWIDQDKAAQTRQDMSDIIYGDSESYRHMCRFESGFFWRNQLLDEFDWYWRVEPDTKLSCDIPEDPFQYMNDNKKIYGFTISIHEYESTIPSLWSVTKDFVSKNPEYVNEDNMLDFISNDDGDTYNLCHFWSNFEVANLNFWRSEQYKSYFEFLDQSGGFFYERWGDAPVHSIAVALFLPKTSVKFFDTISYFHPPYIACPRDINVWQQYNCDCNPEDDFTFEDYSCGKEYYKANNMKKPKTWRKGNIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R864</accession>
    <name>A0A1E5R864_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cell division control protein 42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g3179</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R864"/>
    <dbReference type="FunCoup" id="A0A1E5R864">
        <property type="interactions" value="948"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R864"/>
    <dbReference type="InParanoid" id="A0A1E5R864"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01874">
        <property type="entry name" value="Cdc42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000236">
        <property type="entry name" value="Cell division control protein 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037874">
        <property type="entry name" value="Cdc42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 13" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ83102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="191" mass="21422" checksum="A9DC87C9E6B9D388" modified="2017-01-18" version="1">MQTLKCVVVGDGAVGKTCLLISYTTNQFPADYVPTVFDNYAVTVMIGDEPYTLGLFDTAGQEDYDRLRPLSYPSTDVFLVCFSVISPPSFENVREKWFPEVHHHCPGVPCLIVGTQIDLRDDKVIIEKLQKQRLRPITRDQGERLAKDLKAVKYVECSALTQRGLKNVFDEAIVAALEPPVIKKSKKCTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R883</accession>
    <name>A0A1E5R883_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Transcription initiation factor TFIID subunit 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3292</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat TAF5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R883"/>
    <dbReference type="FunCoup" id="A0A1E5R883">
        <property type="interactions" value="905"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R883"/>
    <dbReference type="InParanoid" id="A0A1E5R883"/>
    <dbReference type="OrthoDB" id="10266330at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08044">
        <property type="entry name" value="TAF5_NTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.500">
        <property type="entry name" value="TFIID subunit TAF5, NTD2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007582">
        <property type="entry name" value="TFIID_NTD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037264">
        <property type="entry name" value="TFIID_NTD2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF1">
        <property type="entry name" value="CANNONBALL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04494">
        <property type="entry name" value="TFIID_NTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160897">
        <property type="entry name" value="Taf5 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="TFIID subunit TAF5 NTD2" evidence="10">
        <location>
            <begin position="181"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="520"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="580"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="622"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="664"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="706"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="756"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="368"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="18"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="133"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="399"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="413"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="437"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="11">
        <location>
            <position position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ83098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="857" mass="95051" checksum="67045EFDBEEE5D9C" modified="2017-01-18" version="1">MSSKPTQSSGAGTPTSKQPPGSQSTQQASASQSQAQPAKSQPQPPQQQQQQQQQQQQQQQQKQQRGRTGGPTASGSGSGGGFSSQDLNKIVLEYLSKKGYHKTEQMLRAESSRTLTPTAAAALPGAAGTSKSAGEKLGKEQKPVPKQSTYVAENQHKAAPVSNPPHPQKRDFEGRVITNFGPNDYIRAFYKLKLWVDQSLDIYKNELVRILFPVFIYTFLKLIQINHPLHARKFFDSFMQDFQLEHNEELNKFFSINSVDHLKENEHVLSWLNNKYKVSITRTSMNLLLYFLIEMDNDYGSMIISIINLHLQPDFIPSTDSGNILNDGIIASSETDNLDTLNSQKVKLAKLPMDSEFKKEVEYELKRQDELQQKETPQSLPTKTLVEEFKDINNNTATGKEDESKTQAKNENETNASGNTNSTITGSENSGSNANDSTTTTKEISSSSSGTGVSATGALSAGSFSTAKKFTEEDIPDSIPLPIKSALDIKLEIQKVKESRDAISIDNLQIPSPSVCMYTLHNTNGDMTCVSYSADCTIMATGFQDSIIKVWSLDGKPLVSLVDEDLMFQDEINGKSCRTLVGHSGPVYAVSFSPCGKYLLSCSEDKTVRLWSLDTFTCLVSYKGHNHPVWDVQFSPLGHYFVTGSHDQTARLWSCDHIYPLRIFAGHANDVECVKFHPNGCYVFTGSSDKTVRMWEVLTGNCCRLFITSQSAVTCIEVTNDGRWIAIGSDDGTISIWDIGSSKKLKVLRGHGRKPIYSIQFNKEDSILVSSGGDNSCRVWDLKKMTVEPNLEPEEQLKNYYGDLESSLQADIKLYGKKKIIQPSLDLVSTFYTKKTPIYKLKCTRSNLIFGAGPYLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAI1</accession>
    <name>A0A1E5RAI1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAI1"/>
    <dbReference type="InParanoid" id="A0A1E5RAI1"/>
    <dbReference type="OrthoDB" id="4070475at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020998">
        <property type="entry name" value="Med3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11593">
        <property type="entry name" value="Med3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="427"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="237"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="271"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="304"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="434"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="472" mass="49892" checksum="2D62DD38DE2CCE6B" modified="2017-01-18" version="1">MAIETVPAGNASGSQTEKDMIIPADLQSLQKYLLEQVPQSEPNNKSASASIALNGYMAKVQNEILPLRLEFNALLKQMAHLDNPQESGKQKYLALKARLVTVTTKINDLSQEFKKLQPLLYILKNNQKILETPNSYYILETLDQLPQPEIKVPLPKQFQPKIINTLPNAVSNSTGVNTAVSSAQKATKSPVNSVNSPSTPATAKTPGSVAASNTVKHSLQGNLASASTFSGIRKNAKGSGANAHASSGSVKKPATNSKRGTPQAFSPLSANTPVTNVSASNNSTNLSNIGAVYNSGGRSKNHDQVTSAPLSKANSANESPSMYMNTSNITPANILQNLNSSMGNNSQSSNMGALSSRVGMNGPSGNIGQQRVNNPNSNSNSNNINNNSMNYGNNMNNRVPSTDNSMGGYGYNGNFSMSPSSILQNMNNLPNNNSSVASGNMSPNGSLNTNNNSAGMLDLNTLDLSNLDDFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB20</accession>
    <name>A0A1E5RB20_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TMEM14 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3065</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RB20"/>
    <dbReference type="FunCoup" id="A0A1E5RB20">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RB20"/>
    <dbReference type="OrthoDB" id="5620at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1740">
        <property type="entry name" value="Transmembrane protein 14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005349">
        <property type="entry name" value="TMEM14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044890">
        <property type="entry name" value="TMEM14_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12668:SF53">
        <property type="entry name" value="TMEM14 PROTEIN HOMOLOG YJR085C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12668">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 14, 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03647">
        <property type="entry name" value="Tmemb_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ84095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="11149" checksum="BBDA5582647CDC32" modified="2017-01-18" version="1">MEHPSFTLAGLCAVGGTMGFLKKKSVPSLVAGLTFAGIYGYAGYLLKQNADNGIEIALGASALLLATGVKRSLPSRFTKPVPLVLSVLGLAGTSYYGLKYKQFYLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBF6</accession>
    <name>A0A1E5RBF6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Histidine--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="3">6.1.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Histidyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g3056</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the aminoacylation of histidyl-tRNA in both the cytoplasm and the mitochondrion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(His) + L-histidine + ATP = L-histidyl-tRNA(His) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBF6">
        <property type="interactions" value="886"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBF6"/>
    <dbReference type="InParanoid" id="A0A1E5RBF6"/>
    <dbReference type="OrthoDB" id="1906957at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00859">
        <property type="entry name" value="HisRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000015">
        <property type="entry name" value="Histidyl-tRNA synthetase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000021">
        <property type="entry name" value="Probable histidine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033656">
        <property type="entry name" value="HisRS_anticodon"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11476:SF7">
        <property type="entry name" value="HISTIDINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11476">
        <property type="entry name" value="HISTIDYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="15">
        <location>
            <begin position="72"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="30"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="41"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="363"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00058343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ84229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="571" mass="64036" checksum="8042927460C1D33A" modified="2017-01-18" version="1">MFLGGAVRNILTRRTFPNTRSPTLRFALXKNQTPNTVSRTTMSTSAQASEQQQKEQQPTKEKLQFTLKTPKGTKDWADKDMVIREAIFNTLSTLFKKHGGITLDTPVFELKEILSGKYGEDSKLIYDLKDQGGELTSLRYDLTVPFARFVAMNGLEKIKRYHIAKVYRRDQPAMTKGRMREFYQCDFDIAGSYETMVPDAEILCVLVEGLTNLGIKDFKVKLNHRKILDGIFEIAGVKEEDVRKISSAVDKLDKSPWEAVKREMCEEKGQTEETADKIGEYVKLNGSLREISEILSKDDKIMNNEKTKQGLEELNLLTDYLEAFDVAHYITFDLSLARGLDYYTGVIYEAVTAASAPPKNAADLKKKAAEKQAKNKKKNDSTEDEDASAFVGVGSIVAGGRYDNLVNMFSEASGKKANKIPCVGLSFGVERLFSLIKQRESLAKNIRPTSTQVFVMAFGGGKDWTGYLPERMKVAKTLWDAGIETEYVYKSKVNPRKQFDAADKCGCPLAVILGKEEYAEGKLRVKRLGPEFADDDGELIDIENLIPLVQEKLSQVHENGVDEVTKLLKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBG0</accession>
    <name>A0A1E5RBG0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="9">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g2854</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RSP5/NEDD4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="9"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBG0">
        <property type="interactions" value="664"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBG0"/>
    <dbReference type="InParanoid" id="A0A1E5RBG0"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000011">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000017">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000053">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000156">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000005">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024928">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF440">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NEDD-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001569">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 9 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="233"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="348"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="404"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="15">
        <location>
            <begin position="493"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="132"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="261"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="139"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="188"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="270"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="285"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="319"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="10 11">
        <location>
            <position position="794"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR001569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001569-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ84235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="826" mass="93742" checksum="EAA9506782270188" modified="2017-01-18" version="1">MSTIFVKLVCAESLYRRDVFRSPDPFAVLTIDGAQTKSTSAAKKTLNPYWNESFKFVSVNENSILTIQVFDQKKFKKKDQGFLGVINVRVGDVLGHLDEDNIQGQTQEETITRELKKSNDGTSVTGRLILSLSKNQQGASQTRTTTPASAAPGTTHSTSANSAQRRVSXARSTRTTGSATSNGTGQPPSATAANGASAGSRRASTTTTQQTPASQTQQQQQPRQYSSFEDQYGRLPPGWERRTDNFGRTYYVDHNTRTTTWKRPTLDASQQQQQQQQVQPLGPTANGTAPSLVSSASSNNNDERRQQHRGRTLPDGSVSDATGASSTSNSSQQVAALAATGSTTNGLGELPPGWEQRYTPEGRAYFVDHNTRTTTWVDPRRQQYITTYGPTTSTIQQQPVSQLGPLPSGWEMRLTNTARVYFVDHNTKTTTWDDPRLPSSLDQNVPQYKRDFRRKVIYFRSQPALRILPGQCHIKVRRKNIFEDAYQEVMRQSPEDLKKRLMIEFEGEEGVDYGGVSREFFFLLSHEMFNPIYCLFEYSSHDNYTIQINPNSKINPEHLNYFKFIGRVVGLGVFHRRFLDAFFIGALYKMLLKKKVVLQDMEGVDHEVYNSLKWIMENSIEGILDLTFSIDDDNFGEIVTIDLKPDGRNIEVTDENKKEYVELFTQWKICDRVHDQLQAFMDGFNELIPEELVTVFDERELELLIGGIAEIDVEDWKKHTDYRGYQESDQTVQWFWKIITEWDNEQKARLLQFTTGTSRIPVNGFKDLQGSDGPRRFTIEKAGEPSQLPKSHTCFNRVDLPPYDDYESMKQKLTWAVEETIGFGQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBX9</accession>
    <name>A0A1E5RBX9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Golgi to ER traffic protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2603</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WRB/GET1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBX9">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBX9"/>
    <dbReference type="InParanoid" id="A0A1E5RBX9"/>
    <dbReference type="OrthoDB" id="69461at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043529">
        <property type="term" value="C:GET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071816">
        <property type="term" value="P:tail-anchored membrane protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028945">
        <property type="entry name" value="Get1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42650:SF1">
        <property type="entry name" value="GUIDED ENTRY OF TAIL-ANCHORED PROTEINS FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42650">
        <property type="entry name" value="TAIL-ANCHORED PROTEIN INSERTION RECEPTOR WRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04420">
        <property type="entry name" value="CHD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184711" evidence="9">
        <location>
            <begin position="23"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ84083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="240" mass="27283" checksum="4B4DBB02B9545EE1" modified="2017-01-18" version="1">MELHLLLTIFFILLTRVSNVYSIPENVVLYLLAPAEYKVELKNYQKILNEKTELKAEQSSISAQDQYAKWTKNNRRLEKMDKELSAKKTLIQSFKTVADQRTKYLKIALVTAPFFIVKFYYGKKLVYLFEEDHLSPMLPKPLQVVCTLGFVGVPMAIVKKVLSEILGKKTASTESAVLATSGVSLGIWIWALEKIISEIIFVYKNIFVKIXEPKTLNNPVEIVTKNPDVELIAPTSAELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDI8</accession>
    <name>A0A1E5RDI8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcription factor tau subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2440</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDI8">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDI8"/>
    <dbReference type="InParanoid" id="A0A1E5RDI8"/>
    <dbReference type="OrthoDB" id="6021743at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024764">
        <property type="entry name" value="TFIIIC_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12660">
        <property type="entry name" value="zf-TFIIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012023346" evidence="1">
        <location>
            <begin position="16"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor IIIC putative zinc-finger" evidence="2">
        <location>
            <begin position="591"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="659" mass="75274" checksum="F78DCB2D3F343E19" modified="2017-01-18" version="1">MPSAGLFTVVGFLHAHCLVDHMNKEQTPNKTSLLLQMKNIYSQKNELVISRREVQYWYDSLCWLDNGIFIINTQPHLTILKTKYQLSHEQNQENNTDMPLKDMLEMDSFVLNLDGQNKFKKDVDTYGNTLLNTEVESFPRQIISGAGGYLAVITNHYNVLIYNQDFELLVNLDLPLTEQPKDVASLFKFNKSRIFHCALWSSDLSFIAVGNENGAVLLFNIRGELMETIPVAEDKSIWVTHLALSEDNLYVACGLADNSVFLHNRKTGESHQIKKQSRFLLSDMKFVCATGHRSGDTVVLVTDAAQIHAYDVFRNNYSCSVLENLGYDQKKXLKTKSSVYILSSCACVKVFVDEECKLSFQLEEKVNTFLKKKIQKFNYIKNENNEYATKMDLYGSCLSRDGSTLALVYTPELIALKYSILSASSFKLSLIPLDESWQINPSFPQSGGLWYQIFHVYDKALPQNYQPLLASSQDNDNIKFDTGLPFNEYLRKYVLRENMGLKFEVLLDQGQSMNKILLLYCKCLYVYAQSHMETLTNTLDAFCVHTVTELLGDATLLDKKTLLKFDMKSEFITQSFDGRAPGTKVPEDTWAIRSLSNHEWKRCGITFLPLVSTALRVCPVTNTRFIDFTKDSFNEYGWFTKTVLETLGDESIYANVKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDJ0</accession>
    <name>A0A1E5RDJ0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DNA polymerase V</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1798</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDJ0">
        <property type="interactions" value="404"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDJ0"/>
    <dbReference type="InParanoid" id="A0A1E5RDJ0"/>
    <dbReference type="OrthoDB" id="342531at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000182">
        <property type="term" value="F:rDNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007015">
        <property type="entry name" value="DNA_pol_V/MYBBP1A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13213:SF2">
        <property type="entry name" value="MYB-BINDING PROTEIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13213">
        <property type="entry name" value="MYB-BINDING PROTEIN 1A FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04931">
        <property type="entry name" value="DNA_pol_phi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="316"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="786"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="324"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="792"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="852"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="898"/>
            <end position="916"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ84968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1125" mass="128470" checksum="9873FCD7B68441A8" modified="2017-01-18" version="1">MEQPSKEETFVTKVNRDLFYTLASDLDSERAQSVINVIDQLKSIQKHDDATYTKEMNYTIDRLITGLGSNIGSARIGFSLCLTEIIDISLDDGITSDVHQYIKKLQEKLPLNDSNNGNKKKVKGKDERGFVFGRLFGLQVLMNEPLFSKLFLLGNDEEKTLNSAFVLEFASMLIDLSSFKSWIKETALFTLYQFLHKLEQTGLNNAQNVLQILELLDSTNITMTMEGLAIYLLFGDNEKTYDLTSFKPSNLHWENNDPLNKTNMSLLIKVLKDVPVSDSDSNQDLKLQQKGFWTPRLHFVFPILIERLLRIEDTEDQDLGPPTKKQKKNDKSAKKHKKQLAAQKRFTIDDLYKKVVDESYFSEKSSNERKYIGFLIFKLCLETFPVEKVQLLFKQQNFLRTLINHASNKQRMLHKLCSSLLNKQIPVIIQDQPEKITPILENIWFYKHNGFLINFDQLTKSKFTSNLLSHQSLEENNLTAISKMLNHHLHEILDNIYLLKSEGSAGDEDKDSEDYTKSLNMYKFCIDSLLHLVRSHKSQLSKKWILPIIKSLAKVSYFRNNEKKILSADLNHDEETRDEAKVLANQNDVHTIALERLYSILFDVLSSQNEITSKGKPVVWSLQLLKEIREIEEQMSADCLLHPVDSSLVQVKXAALKDLEDIQQSLSSDKTPSAMRNVLQGLQQLLVLNLLQLYSGNSEAIAVLEDLSSFYEDYSEKCSQNESVYDAINFVSITEILMSSMSDEKALFRKLSLDSWDMFVKEIGTQELEILLETLTTRENKSGFSHLFGADADMEDVXEEDDDGEDKDENYDDGAEEEEEEEEEEEEEEDEQEGTDTDSSDSENDENGNHSNENENSXKVEKETTSALAKALDLPEGIVDENGNVNHDGYVDMGSGDDFGEGEDEEEEEEEEDFDDEKMLELDKTLSMIFKTRKEALSGTPTGNKRKIEVQKARENVVNFKSRVVDMLEIYVKYVDKNALDVNKLETLLYMLPPMIQCLKMTLSKPLVDKLGKLIKARLTKIKVNASHSDAHLFDVAVTTLQTIHDLASSNKSGQYSQLFYSVCSICSLFVSKIIFLSSADNTFDAAIETYSNTMKQWSKNXNCKTPATFFADYVNMLATKKHQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDN4</accession>
    <name>A0A1E5RDN4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Genetic interactor of prohibitin 7, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1782</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDN4"/>
    <dbReference type="InParanoid" id="A0A1E5RDN4"/>
    <dbReference type="OrthoDB" id="4069787at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ85018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="314" mass="36235" checksum="123911DE868CBB87" modified="2017-01-18" version="1">MPSKHIAMYARRLFVQSKLGTKSFERYASTNSKVPKSLLLKQEKRLTQSNLSAEELKQIETQKLKNQTSQLLITSLKDISGYFNPGGATSNEDDLEAEHFDKLRKKYENGYLDRLLKEKFGVELQNSDILIASNVNEKLWASSFENITFEDLEILNYYLDVVLLENYGYAWSNLPKDLQRLLFYRAYGSYGPRNDLENTFATKLVKPQDFLWLKPSPSVFSQTINISSRKPHKLNLISDIKNVDLSKVGNGLINIYECSDKRFDQWKNKFNKADPGSKFVLYLMGVVSLFCLYKDKQFYSTLESSESDSEPVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDR1</accession>
    <name>A0A1E5RDR1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein SCD5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2227</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDR1"/>
    <dbReference type="InParanoid" id="A0A1E5RDR1"/>
    <dbReference type="OrthoDB" id="2553626at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013182">
        <property type="entry name" value="DUF1720"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08226">
        <property type="entry name" value="DUF1720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="DUF1720" evidence="8">
        <location>
            <begin position="480"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="128"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="394"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="625"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="725"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="16"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="128"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="176"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="396"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="625"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="641"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="678"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="702"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="731"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="766"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="794"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="812"/>
            <end position="848"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="884" mass="98228" checksum="16E5322F21522016" modified="2017-01-18" version="1">MSYEWLDNPAVDQAVPSYFNPSSQNPPSNGNPQHGFSQSTYPIQNQSASVNTQNSSYQAPFSSQSPQLPQVPPRTNMGVLASDIFKNPTYSLPTQSPHFQTSSQKPNFTQPAEEANAAAIYNYFSNPGVSSKHQADSQTSGRSKPLNMNNPFRSANTDXSNLLTLPNQKTGPARLSSGGTSSFQSSRSETSIFKMNSNSDSPQVSEIPLALSTYQLSLEESKNYLRWYQYISDKSNFKSYVTLQDIFNFLKTNFEITDHIKKVVSEFFRNIKERIGLENFXAVLRFISYLLLKGQIPTKDMLLLAAPVLKPKSILSKGEKEEVYEEIEDSQNDQPFDFDNFASLLLTGKSHRVKRVVKRSDNRPTKNVRFSEKLETFEKNELPEIESPIVEKYLQPGSSSSNTSNNDQPSENLKNEPLDFSLPMDQLLAKLQQPKQPATKEEEEELEDMKESLTHFQNLPKIDSVSLHLPSRNYNDPNEIEPLKPTATGSANHLFGQQPLQPTATGSANRLFGFNGNKSGLENSAMEPLKPTATGSANHLFSNQSGLPSAGTNFDSGNPTTTFGNFESNALKPTATGSANFYMKHSNDYIQSHPQTFNXTLQANNFGNNSSNGNEYLNQLKLSPTHTSNGTFAQNSNFSGNTQNTVDDNNLTNTNQNNSFQNXSNEFTFNLPNKNHLAGNSSSGLQAHNSGGATSFGAPFSNIPQQSRIDQTNVQNKNKASDYFSMLLQPNGNGSQNNSGSSLHLPNSMSPSSLQQSPMHQGNIMQQQHTVQPQFQPQQPQQKHNTGPLGYQYTGYSNTNFQPNGAPQLHTQQQNSSFLSQQQQQQQQQQQQQQQQQNFSNGFQSQNSTFLGNRPRTNSAAILGDMSQMQQQLNNMQNNRYGQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDR6</accession>
    <name>A0A1E5RDR6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Iron-sulfur assembly protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2310</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the assembly of mitochondrial and cytoplasmic iron-sulfur proteins. Probably involved in the binding of an intermediate of Fe/S cluster assembly.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HesB/IscA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDR6">
        <property type="interactions" value="189"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDR6"/>
    <dbReference type="InParanoid" id="A0A1E5RDR6"/>
    <dbReference type="OrthoDB" id="333486at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.300.12:FF:000001">
        <property type="entry name" value="Iron-binding protein IscA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016092">
        <property type="entry name" value="ATAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000361">
        <property type="entry name" value="ATAP_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050322">
        <property type="entry name" value="Fe-S_cluster_asmbl/transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017870">
        <property type="entry name" value="FeS_cluster_insertion_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00049">
        <property type="entry name" value="iron-sulfur cluster assembly accessory protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10072:SF41">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY 1 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10072">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01521">
        <property type="entry name" value="Fe-S_biosyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01152">
        <property type="entry name" value="HESB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core" evidence="5">
        <location>
            <begin position="140"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="54"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="87"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00054873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00071673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ85047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="243" mass="26790" checksum="526350EEBA92B0E4" modified="2017-01-18" version="1">MSLNVPIMMRSYMRVLKTDXFIVSQPLMASTKPGWASVRYINMSYPARLRTNFDSRNTDPNDSSAVLHGLNNSSSSHNNSAHGTGASEHSTTGWSSYSLPSRETLKNLGKKATSKKSETSTATGTGLRKKRKLRPRKAFISLTPNAVVHLKNLLNSPDPKLIKIGVRNRGCSGLTYDLSYIQEPGKFDEVVEQDGVRVVIDSKALFSIVGCEMDWLDDKLSSRFVFKNPNSKGTCGCGESFHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDS3</accession>
    <name>A0A1E5RDS3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Alanine--glyoxylate aminotransferase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2346</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDS3">
        <property type="interactions" value="1326"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDS3"/>
    <dbReference type="InParanoid" id="A0A1E5RDS3"/>
    <dbReference type="OrthoDB" id="7403325at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008453">
        <property type="term" value="F:alanine-glyoxylate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004760">
        <property type="term" value="F:L-serine-pyruvate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019265">
        <property type="term" value="P:glycine biosynthetic process, by transamination of glyoxylate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152:SF24">
        <property type="entry name" value="ALANINE--GLYOXYLATE AMINOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152">
        <property type="entry name" value="AMINOTRANSFERASE CLASS V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="6">
        <location>
            <begin position="281"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="114"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="123"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="179"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="588" mass="65669" checksum="49792E3CFFF32337" modified="2017-01-18" version="1">MSIEEYITKHKESAHQDPHMVDVPKELQEQLERENGKEGETPSSSSSAAFSPQFNEHVYIPGPVSMSEDVLKALGVQSQSHLGDEFATTYRXALQNLYKVVQLDVNSAEYMAGDAGKGETDGHNTPNHTASSSHTAHDSSANHHPGNAISHAGSLLGHAMDRTPSGRSVPPSPHKMKKTDKESTPVLEKSKQKSYQSFVLSGSGTLGFDLIATNLMVNKPSITKTQQRALVLSSGYFSENMYKCLKQYYPDTHDTKNYNMVTSNVDILYCPTGTSKFNHHELKFLLTSNNYQVITVTHVDTSTGVLVDLYTLLPFIREHAPNAFICVDAVCSLGVEQIDLNVLSQVDFMFSVSQKGLGCPPGLIVGYMGSRVLEYLANPDINKQIKGFYTNLHKWIPVWESYEYYKLQIPGTSAALSEFNTEKPKYFATPPTQLIHALNVSLKEILDFGIEKRCKAFAELSNYVKQQVHDLFGLELLTEHADSKQAAHGLTCVLYKNSMALVKYCHDTFKIDLAGPLLRNIKLKNSNIFKIEYFRIGHMSEALLKDFVENEHKNLDVFLKILNKATETVDLNENRKGYSSISLVNMEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDS4</accession>
    <name>A0A1E5RDS4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Alpha-1,3-glucosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1814</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the ALG6/ALG8 glucosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDS4"/>
    <dbReference type="FunCoup" id="A0A1E5RDS4">
        <property type="interactions" value="965"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDS4"/>
    <dbReference type="InParanoid" id="A0A1E5RDS4"/>
    <dbReference type="OrthoDB" id="5589195at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042281">
        <property type="term" value="F:dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004856">
        <property type="entry name" value="Glyco_trans_ALG6/ALG8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12413">
        <property type="entry name" value="DOLICHYL GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12413:SF1">
        <property type="entry name" value="DOLICHYL PYROPHOSPHATE MAN9GLCNAC2 ALPHA-1,3-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03155">
        <property type="entry name" value="Alg6_Alg8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="244"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="379"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="445"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="498"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="529"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="565" mass="64939" checksum="CA2F03E218CE393F" modified="2017-01-18" version="1">MGSSSKKPETLVLEARNDATKESAATTTQTGIESKEAFYASPLYDFLSPFKLVTHQWEAKYIIVLFALILKCAVGLGSYSGYNTPPLFGDFEAQRHWMELTINLPLSQWYWFDTHYWGLDYPPLTAYHSYILGYLGFFLNNKRANWFTLDESRGHEDLDLKSFMRFSVILSEVLLYIPGVIYMCKWIGKQTKQSPINQYIAAAAILLQPSLILVDNGHFQYNCVMLGLTVYAINNLLDEFYAAAAACFVFAICFKQMALFYSPIFFAYLLRKSLFQWNLGRFLLIGLATVSSFVLCFLPLYLLGGGLSNVIQSIHRIFPFGRGLFEDKVANFWCVSNIVVKYREVFSNEELKLYSLVLTLAGLLPSMLVIFFWPKKHLLLYALASCSMSFFLFGFQVHEKTILLPLLPITLLYTSSNRSVVSMVSWINNVGLFSLWPLLQKDGLQLQYVVIFCLSNWLIGNFSFVTPKSLFPKWLTPGPSVSQVSATYKRRTLLPRSIFWKLVIIISYVAMGITHMLDLWVLPPEKYPDLWVVLNCSVSCMCYMLFWLWTNYKLFSLRNQSFQSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDT6</accession>
    <name>A0A1E5RDT6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation factor G, mitochondrial</fullName>
            <shortName evidence="10">EF-Gmt</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Elongation factor G 1, mitochondrial</fullName>
            <shortName evidence="10">mEF-G 1</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Elongation factor G1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MEF1</name>
        <name evidence="12" type="ORF">AWRI3579_g1952</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Protein biosynthesis; polypeptide chain elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDT6"/>
    <dbReference type="FunCoup" id="A0A1E5RDT6">
        <property type="interactions" value="662"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDT6"/>
    <dbReference type="InParanoid" id="A0A1E5RDT6"/>
    <dbReference type="OrthoDB" id="198619at2759"/>
    <dbReference type="UniPathway" id="UPA00345"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070125">
        <property type="term" value="P:mitochondrial translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01886">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16262">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01434">
        <property type="entry name" value="EFG_mtEFG1_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04091">
        <property type="entry name" value="mtEFG1_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000003">
        <property type="entry name" value="Elongation factor G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000001">
        <property type="entry name" value="Elongation factor G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000022">
        <property type="entry name" value="Elongation factor G, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000015">
        <property type="entry name" value="Elongation factor G, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000558">
        <property type="entry name" value="Elongation factor G, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00054_B">
        <property type="entry name" value="EF_G_EF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009022">
        <property type="entry name" value="EFG_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047872">
        <property type="entry name" value="EFG_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004540">
        <property type="entry name" value="Transl_elong_EFG/EF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00484">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009381">
        <property type="entry name" value="PRK12740.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43636">
        <property type="entry name" value="ELONGATION FACTOR G, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43636:SF2">
        <property type="entry name" value="ELONGATION FACTOR G, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 12" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="69"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="78"/>
            <end position="85"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="149"/>
            <end position="153"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="203"/>
            <end position="206"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ85062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="771" mass="85558" checksum="1C6499F94C549ED8" modified="2017-01-18" version="1">MSATVMKQFGPSATMRSALGRSIQSSFIKPTHRLFSSTLSKRMEYEEEKEIVDKIRATELKPDDLARCAKLRNIGISAHIDSGKTTFTERVLYYTGRIKAIHEVRGRDNVGAKMDSMDLEREKGITIQSAATYCSWDKDNEKYHFNLIDTPGHIDFTIEVERALRVLDGAVLVVCAVSGVQSQTVTVDRQMRRYNVPRITFINKMDRMGANPFKAIDQIEAKLKIPCAAVQVPIGLESNLKGLVDIINRKCVYNEGIKGEVLRVDENIPEDLKDLVEERRALLIEKLADVDEEMAELFLEEKEPTVEQINNAIRNATIARKFSPVLMGSALANTGIQSVLDAVVNYLPDPSEILNKGLDVANEEKEVTLIPNDKLPFVGLAFKLEEGKFGQLTYIRVYQGKLKKGGFITNVKTGKKIKVSRLVRMHSNDMEDVVEVGSGEICATFGIECSSGDTFTDGTVSYSMSSMFVPDAVISLSIAPKSKDGATNFSKALQRFTKEDPTFRVKYDSDSKETIISGMGELHLEIYVERMKREYGVDCVTGRPEVSYRESIQIPSEFDHTHKKQSGGAGQFAKVVGDLTPVEVQEGEPIASENSFKSKVVGGRIPEKYLAACEKGFIEACEKGPLVGAKVIGVDMTMIDGAIHVVDSNEFSFKFATMTAFKEAFMKAQPVLLEPIMKVDVTAPIEFQGSCISLLNKVNAVIIDTENGQDEFTITAECSLNMLFGFATTLRASTQGKGEFSLEFKHYAPTLPNIQKEIVDNYVKEQSKKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE20</accession>
    <name>A0A1E5RE20_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative transporter MCH4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2375</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RE20"/>
    <dbReference type="OrthoDB" id="6499973at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032218">
        <property type="term" value="P:riboflavin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF177">
        <property type="entry name" value="RIBOFLAVIN TRANSPORTER MCH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="127"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="150"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="290"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="360"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="390" mass="42442" checksum="AA5262C42E98E148" modified="2017-01-18" version="1">MSRQSGLNFSPSKDTXVLGETPGDXVLSRTSSFSEEALDEKQEFNHDNNFPDGGRQAWTTVGGAFLGLTSVFGVLNSVGAIEAHIRDNQLREVPTSTVSWIFSIYTFMLFFSGIFCGCFFDRNGCSYINHIGSAFSIVGLYTLAVSTKVWHFILSFSICYGLGSGILLTSLISAASTWFRKKSNTAQGLISLGGSFGGIVFPVMLRCACAESGTTVAATYFASYCIHKGYTAAQSYTFITVINAFGILGRSSGWLADRYTGRYHILFATMLLASIFCFVLWLPFGNHMKVMYAYCALYGCMVSGFLSLAPAAVGAISKIEEFGKRFSTMYFVVSVFCIPILEIGGVIIGDSKSQSSFNHYVIFCSCILLLGAISYTITRILHLGLKAGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE42</accession>
    <name>A0A1E5RE42_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative pyridoxal reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2340</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE42">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE42"/>
    <dbReference type="InParanoid" id="A0A1E5RE42"/>
    <dbReference type="OrthoDB" id="37537at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050791">
        <property type="entry name" value="Aldo-Keto_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43625">
        <property type="entry name" value="AFLATOXIN B1 ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43625:SF78">
        <property type="entry name" value="PYRIDOXAL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="17"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="335" mass="37891" checksum="DA0B447EDF6DF9B7" modified="2017-01-18" version="1">MSTAKIIKENLLKSSTLGYGLMSFTWRATPIPIEQAVATMGTVMKTIPPQSHIMFNVGEFYGEPLKNLKYCQXFLDTLSKQERARILVSVKGGVDLKTFAPRGSRADVRKSLDNCLTAMPYIDIFEVARLDEANWEETFETIIEDYIKKGKVGACSLSEVTQEQIQKIHEKFGEYVVAAELELSMFSQNILKDGTAEYCNKIGLPIICYSPLGRGLLTGQVKNTKDIPEGDFRKMLARFSDENMKHNSILLRFLEKFVIPGERSLPQVALAWIRKLPESKFPNTNFIPIPSGTTKERVTENFTPKTLSSSEFEEINKFLESFVTKGDRYEIASKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REJ6</accession>
    <name>A0A1E5REJ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2103</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5REJ6"/>
    <dbReference type="OrthoDB" id="3973258at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="325"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="372" mass="42256" checksum="108CE59CD349AEAA" modified="2017-01-18" version="1">MQPRIDFIQIKHLQNEARHARMGPYESHAESRSIDSRPTPAPLPMKGSFQNLHMAHCNPLDLRLDLEAPQQHPTPLNSPDLEVTHSGLTSSFLDDDDESDESDGSCEINGNNKRVSGTKKLALEPLKTQDNLTQTLTQKISTFPLRPHSPKIQKFNNVPKHRSSKNLKSLLKVQEGQLNYVVYSSKDNLSDATIFGTQINILQTVAYHSQQRQEQKKQARQQQQQQHLQEKGSNENAQESVNPAIPLPQTEYETVSIRVNKSIKEEYKKRKRLEDRGGYYASVNNEQVSVDDDMLENQDNGEQQLAQEQDEYALIKAFKLACGKSNDSTASQNQHNKNDHRVVGPSSPHSNDDKXSQKKKKHRHLVWDLSHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RER9</accession>
    <name>A0A1E5RER9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Putative transcriptional regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1701</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RER9"/>
    <dbReference type="InParanoid" id="A0A1E5RER9"/>
    <dbReference type="OrthoDB" id="2595934at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051089">
        <property type="entry name" value="prtT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR OF PROTEASES PRTT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="20"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="246"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="638"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="266"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="815" mass="93202" checksum="CEC9EA229A2438B5" modified="2017-01-18" version="1">MNSSSNEGPVKKKLPRSLKACDRCKIKKQRCEMSLNVSMDVRCVACQKVDTKCSLQELLQPQLSSYTLNPDAEADGENPENSDSLKENIDIALTNNDLNPVDGNVFLKKILEDKKLKMSFENKIKKPKTASNAALQKKALSTSIYTQHYVNSFGTSTIDEINAKVSKILQYLEAGTNVTHPTVPLFSNATESFSNDLGSMNNEDGAAPIVHDMLQLPTTNSKPSSGSEQPLISISNVFDEENQNVPIATSDASRSNEDTPLPEQRQTPEDKNNYKNSIDKHVLLHTEKHLSKPEPTFLLSPFATFSRLVQKLNVPLQVRYLHDPVPLDEATEVPVVDDVISSNILTLPEVIMLVREFKDNYGTWVSFPENVPVEDIVDAIRKSHCSVLLTTACVLALRYTVYYHDLKTRIYKNLLFKLKYDLEKKLVSNDFSIEFIQSLVMMSIYSSSLSSDIQTFDAWSLSAMGIQFYISHGVLNAFKNVPVSPLTTTSSATETNLKDNLNTDEQSMLERLRNHRLWNHLVLVHLTYSVLSGRMSILNKRSLRICFDCLELPNATHFDGRMIAEISLYSILYEYTQNFSDVKDVKEPLIIIDAWYAKWNYLTTQPLEQFVDFSYHFSYVLMIYMWYHHKKFPXVESQRKRQSKKSKEQEQKEQASFTNANGFGAQRTFNGERLNRKFPLSDVFGLIPLETQKIMLEHCEKGLKLFVDTRIHNFRYLSDQLIFISVYLSLITLKTLKNVCEEHPXELPQELVKSLIGYVKQLSLKLRNIRESELKSFWVEEVDLRVPSVILQYHSALESYLKETFPEMVDSTKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REW4</accession>
    <name>A0A1E5REW4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2380</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REW4">
        <property type="interactions" value="145"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REW4"/>
    <dbReference type="InParanoid" id="A0A1E5REW4"/>
    <dbReference type="OrthoDB" id="267323at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003873">
        <property type="term" value="F:6-phosphofructo-2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003094">
        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013079">
        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF39">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE_FRUCTOSE-2,6-BISPHOSPHATASE YLR345W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01591">
        <property type="entry name" value="6PF2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="4">
        <location>
            <begin position="139"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="71"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="94"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="649" mass="74117" checksum="692BB10EBEDA07B4" modified="2017-01-18" version="1">MEHNTRTSNGSGMNAILPKIKAENNHMARKTSRWDRADLKKVSNTSSCNNDKGVNQTSNEVPSIKKLKLENATSHSDLNSSADFLPSAGKKRGSPSPSHSSTSVGVDSSSEDYSNIHKDGVHEEQGDYSISPGQLYSTDSGRLFHAGKILIVLVGLPATSKTKLSVAITRYTRWLGVKTQAFHVSEYRRKYCQQRHVEDSTLDSTDSKSKKANILPADYFSVCPQTEEAKKYREDIFKSCMEDIDLFFKEPNSQLAIYDALNILPEDRQTIFNKFAKKNEVSKGKVLYTDDGDSLGVDDVKVLFIESVVTDPELMQRNVNISIGSVDYEGWDPELARKDFMQXLDINKKYYQEMTSQENLSFVKYINYGERLIVNNNFYGYLINKIVFFLMNLKEKKGKVFLSRCGTSDNDKYLDDEQLNEEGVCFSKKLTEVVLKRLNEERMQRIKNSKSQLEQKTFDSNSSLNTLSKPLVETNATVQRHLTGGKAQYKQVSLHDGADEDSFIVWTAPRKRTHDTSKFFQQLGIPVRQRNQLKQLHPGIVTDMLAPEIAKKYPHEYKHFLKNPYHYRYPRAESYHDLAVRIEPLLLEIERMCGDVLIIAHESTLRVLYGYLMACTSSDIPNIDFSRNEVIEISFGHYENQAIRLPIDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REY7</accession>
    <name>A0A1E5REY7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Meiotically up-regulated gene 14 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2005</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REY7"/>
    <dbReference type="STRING" id="56408.A0A1E5REY7"/>
    <dbReference type="InParanoid" id="A0A1E5REY7"/>
    <dbReference type="OrthoDB" id="3238794at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000009">
        <property type="entry name" value="Class II aldolase/adducin N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051017">
        <property type="entry name" value="Aldolase-II_Adducin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004855">
        <property type="entry name" value="PRK06208.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672">
        <property type="entry name" value="ADDUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672:SF25">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 14 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="2">
        <location>
            <begin position="64"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="298" mass="33434" checksum="0C0D4FE733950928" modified="2017-01-18" version="1">MSKTETTSTTTTSNEPEKFNVSTSRGYQLKGTQGVHNIATGTVEYENKKPQFKTKEEEREHALEHMAGAFRVFGRRGYTEGSAGHISIRDPVDPTTFWINPLGKHFSLMKKSDLVHCNEHGEILPDGNTECVINTAGLKIHSQLHKARPDVNAAAHCHSVYGKAWSSFGLELDMLNQDACTFYKKHSVYKDFGGVVLEEEEGQKLAAALGDNKAVILQNHGLLTVGETIDEAAYLFTLLERTCEIQLLVEQTGLPKKIIGEEEAYYTYFNTSDPETLYTEFQPDYQMELKLTNSEFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF71</accession>
    <name>A0A1E5RF71_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Rhomboid-type serine protease 2</fullName>
            <ecNumber evidence="4">3.4.21.105</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Rhomboid protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g2013</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Probable rhomboid-type serine protease that catalyzes intramembrane proteolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.</text>
            <dbReference type="EC" id="3.4.21.105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">cis-Golgi network membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.105" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF71"/>
    <dbReference type="FunCoup" id="A0A1E5RF71">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF71"/>
    <dbReference type="InParanoid" id="A0A1E5RF71"/>
    <dbReference type="OrthoDB" id="10257275at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066:SF1">
        <property type="entry name" value="RHOMBOID PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="88"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="15">
        <location>
            <begin position="49"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="319"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="282"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="330"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="356"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ85527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="367" mass="41860" checksum="AAC18C7387685F56" modified="2017-01-18" version="1">MPYVYPGPTAITSGLIVFMWIVYLSPSMSFLLNPLDLVDHSIGNFFKNLSMYPLDHADLPHLIVNSFSMFVLLPELEMKKGTIRVGVLLNLFAGLTGLVYSLLTLFIKYVVYHNMSVRMIKGSTYWLFTVYTWNCFRLSYKQQNLNKVWEIPSTPYKIKSLQTYPFLALILLNIIFYQHTSFWADLSGVLVGLLYFKFQHFFDVTLCLPRQLLLKIETLNFWTNRQHTGFSLAKFLRFMQIHYVMEDDEPLSQTDSFDNEHSNAGSQDPILPIIRSRRGTLLGSQKPPSNTTRAGDIDGISDANTSTVSLGIASSFPELAEFPQPPSLTKKMSSHSPVRSRRNTLLDSQKPLSSKILKDSPMKSKQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFC1</accession>
    <name>A0A1E5RFC1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Autophagy-related protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Autophagy-related E2-like conjugation enzyme ATG3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1742</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt) and autophagy. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8. The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8 from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8. The formation of the ATG8-phosphatidylethanolamine conjugate is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). The ATG8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATG3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFC1"/>
    <dbReference type="FunCoup" id="A0A1E5RFC1">
        <property type="interactions" value="1080"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFC1"/>
    <dbReference type="InParanoid" id="A0A1E5RFC1"/>
    <dbReference type="OrthoDB" id="1584384at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019776">
        <property type="term" value="F:Atg8-family ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061723">
        <property type="term" value="P:glycophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044804">
        <property type="term" value="P:nucleophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007135">
        <property type="entry name" value="Atg3/Atg10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12866">
        <property type="entry name" value="UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12866:SF2">
        <property type="entry name" value="UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03987">
        <property type="entry name" value="Autophagy_act_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="285"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="288"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ85599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="352" mass="40156" checksum="D9D8F346742FAEB7" modified="2017-01-18" version="1">MLRSTISSWREYLTPLQHHSKYKENGTITPEEFVIAGDYLQSMFPTWKWNGEDMANTNVKDMSIRDFLPKEKQFLVTRKVVCDKRVEVPLEDYDVNDEEQDGKNIKAKSTGSDDELDDEGWVVESLKTLHVSDRTNPEKAKQGNTAFADQANTLGADDEEFVEEIFEEDFEGTIDDFDESNGVEGFESVSATALNATTRYYDLYITYSTSYRVPKMYLVGFDSDGLPLQPKAMMDDITPDYRSKTATIENLPFFKSKMTSVSIHPCKHSNVMRMLMNKKLEGVSLQTHSDEAESREKEVDTKLAANSDLKEEELQDNEDAAGLRVDQYLVVFLKFISSVVPTIQYDFTMEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFH0</accession>
    <name>A0A1E5RFH0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ubiquitin-conjugating enzyme E2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2213</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="5">Protein modification.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFH0"/>
    <dbReference type="FunCoup" id="A0A1E5RFH0">
        <property type="interactions" value="419"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFH0"/>
    <dbReference type="InParanoid" id="A0A1E5RFH0"/>
    <dbReference type="OrthoDB" id="10253686at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23791">
        <property type="entry name" value="UBCc_UBE2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="9">
        <location>
            <begin position="10"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="173" mass="19827" checksum="D8BF8B7E1D616C5E" modified="2017-01-18" version="1">MSQNQRDQQNVTQRLQTELVQMMTKGTPPGLSAFPIDEEDITQWSGIITGPEGTPYEGLKFKILFKFPVQYPFVPPKVTFKSPMWHPNVDMSGNICLDILKTQWSAVYNVQTILLSLQALLEEPNNSSPLNAVAAELWDTNMVEYKKKLMARYEEFDDDDEDDDDDDDDDDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFI7</accession>
    <name>A0A1E5RFI7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Structural maintenance of chromosomes protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2260</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFI7">
        <property type="interactions" value="1025"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFI7"/>
    <dbReference type="InParanoid" id="A0A1E5RFI7"/>
    <dbReference type="OrthoDB" id="10254973at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001301">
        <property type="entry name" value="Structural maintenance of chromosomes 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45916">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45916:SF1">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad50/SbcC-type AAA" evidence="11">
        <location>
            <begin position="27"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="243"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="675"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="802"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="875"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="12">
        <location>
            <position position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ85616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1104" mass="128736" checksum="F830103D4D30DF5D" modified="2017-01-18" version="1">MTAEPSRKKFKVSSPDYSEFAKGAIVKIRLENFVIYKLTEFHLSPSLNMIIGPNGSGKSTFVCAVCLGLAGKPEYIGRAKKIEDFVRNNTQSCKISIFLKNDYGKLSQSTKLRNFIQKNQDIIEVKRIIYKNPLSATNSTRNYNGNKTNQSAKKSEYFINNNPCSESLVKELVSLLNIQLDNLCQFLSQERVEEFAKLKSEKLLTETIRSIQPDLIQVFEGLQNNELVKMEKTKQLRNQMKMLEDSTTKRDELKGKVDELQNYREKIKEIELHKKLIPIVKIKDFDKSRQKLKQELKTIKSHYIELTKKAEPFQSALQRLTEDYKKSQNLYKNSEKEVIVLNNELNHKMKKIKKVVSKIDENKKKIQYYQKRNEACLHQIETEKVKKESLVKQLSNLNSPDQSEFDKLHEQRKLKTIAKNNKVAECETLNDERMSVSSKLSKERQVIQQRQSSLESNDPIHILDTQRFKPEIKTAVLYLREQADDINSTILPPPIMSVSATNVAYSRFLNAVVDRNVAMAFTITSDSSYHQYSSMLLDKWGLNIRQLSTEPVXQPNIDSKTLQQEWGFDGYLLDFVKGEPAVIQMLCEQSRIQQIPVSAKPLTNDIVERLKNFKDQKGNLIFQRVLAGNTIYNFRQSKYGDRQIFYTSSNIHQNNTLYQGNVISEEYKNKILEFIQTRTTNVEAYEQRLHELKEQERAIKNEIDSFVEEVDRVVQEEVRLKQLGKEYRRVQAYIEKADSNISHFNEQLKKDVSSKTQQLLFDNITLSGTLCQQNEEMLRFNVKVDEMNARMLEQKIVIFDKKNKFKHKEDEYEGLNKELEEMDKLYRDKANEYKEYKKNDKVLQWKTEIRQYSEDLLLKLKNLSDHYQDIQKFDTESIREEIENLEDDIKNSNHDDSVINILETTEEKIKVLERDIPKAQQEVFQLEEQMKTDHEFLLPKLEHMVSKISTRFGTLFPNVGSAGAVELDTKKDYHDWNLKIMVAFRDNDKLSPLDSHTQSGGERAVSTVLYMIALQEFTTAPFRVVDEINQGMDSNNERIVHKAMVENACAEKTSQYFLITPKLLTDLHYHPKMRIHCVMAGAWIPNPSEDXEKIHFGQTSNYVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFW8</accession>
    <name>A0A1E5RFW8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Bud site selection protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFW8"/>
    <dbReference type="FunCoup" id="A0A1E5RFW8">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFW8"/>
    <dbReference type="InParanoid" id="A0A1E5RFW8"/>
    <dbReference type="OrthoDB" id="434695at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034044">
        <property type="term" value="C:exomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015374">
        <property type="entry name" value="ChAPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31975">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31975:SF1">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09295">
        <property type="entry name" value="ChAPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="457"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="460"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="804" mass="90648" checksum="2B24945ACC0CD9B6" modified="2017-01-18" version="1">MLLTQGSIPEVQETSINETLRARHSKLGSPALQTYPLGPADLVSLYKYIPVDPNQHIGANNNTSASGNNNNNNNNNNSSGKTNNNEGAEVYEDPLAQIKKKLELGTHFYNSGIDTSDPTSIAVFCKSIKEAIEENGQQLWYGKQKHFRVGHIAYSTYNCFSQCDMNVVVKFPGNIETYLTTSEDGDIALKEDHPGANEELWLETFVSGVVRSFTQMMDNKSEGELNSVVETKLYNPLLMNDINDIAELFITKGFPLCYDRGFTLGGPVDCIGITNLNNYLVEVYCKIVQLSGYHQMAITTLDVMISKMGKKYRELYYLKARILFENNRKMESLDIINEELQSKGKTKKSSTPNGVYKNELILLQINVLYSIVKDKEIQQLENDAQDIALTHQMINKLSKQLCKISPSEFRPWYWLVKSYISLKDFENALLALNSCPMNMTKDKYIFKRMDNSGGYGARNGTGSSASSSSANASTAGSNNANGNSSNSGVAQPGSPESMHLPLPIDVVMEDITGLNSQDVISEHMQAEASLMNLPGSSLKTTYAIAYSLLVDIVNLIGWEKLLVLRSDIFVMEEEFHEINSRKASTSEFETEDLQNGEAGISPGNGVSKSFLKDDKRLCERWLDNLFMLLYQDMKQYTMWQAEQVQFESSGTEYTRSTFEWELLGLLANRLKHFNEGSLAFQVGLKQRFSGVCCKKLIEYYVNEKNRILQNYKLSSGVILKNVQQLNNLILDLCVQNMVWNHRWYIEFSLNLQYAFKSLIEQDGLEKLENEIKSKYSEEVYALCYDNALKFFGNQGVGISDFHAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGG3</accession>
    <name>A0A1E5RGG3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SWIRM domain-containing protein FUN19</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2214</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGG3"/>
    <dbReference type="FunCoup" id="A0A1E5RGG3">
        <property type="interactions" value="133"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGG3"/>
    <dbReference type="InParanoid" id="A0A1E5RGG3"/>
    <dbReference type="OrthoDB" id="5598695at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000087">
        <property type="entry name" value="Transcriptional adapter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374:SF21">
        <property type="entry name" value="SWIRM DOMAIN-CONTAINING PROTEIN FUN19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTOR 2 ADA2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SWIRM" evidence="2">
        <location>
            <begin position="412"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="101"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="509" mass="57614" checksum="03D32BBC50BBB2FF" modified="2017-01-18" version="1">MSVTEFNTPQPEHVELNVCKDDMNHGFKPKVSQDSNGLGEQGLHQVLEPHPSVSSNGTLQEIPKTGNFNPMAVLLAKESSNLSDCFGEMNKKVGVNATSATTDYNNHNTNTKNNNNNKRTNESVVDGTELRSSQGSMGYGTKELLSPPMSPNINNREADDDYTSKNDTLLFNSSDNNKPINHNSYLLLTPQSHHAQLSTHPMTHKSLYQVHGGHTAFNDNSFDGIFLQGHTNDKETQVPCKPDHKISANKPLVADLQWSNKDYSSDLKFFISKYRNYKKLKNGIHMNNRLERTYYTRSNSNIVDNYTNGENLNGNLTNNSVYSNTFHPVRSYRTRLRTIAEGNKDYEPQLSARSIPKKLVKTTHTPGKITKQHIPKPHVAHQPLNEWKQMQDYAPPLASLDNIHNGNSKGIMKVEWKGSTMDLSEDPLRDKLHPAELQLASILRLSCDSYLASKRRLFMEKVSKLKRGQGFRRTDAQKVCRIDVNKASRLYQAFEKVGWLDDKNFVKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGK7</accession>
    <name>A0A1E5RGK7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">DNA polymerase epsilon catalytic subunit</fullName>
            <ecNumber evidence="17">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AWRI3579_g1570</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">DNA polymerase II participates in chromosomal DNA replication.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="17"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGK7">
        <property type="interactions" value="742"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGK7"/>
    <dbReference type="InParanoid" id="A0A1E5RGK7"/>
    <dbReference type="OrthoDB" id="10060449at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008622">
        <property type="term" value="C:epsilon DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008310">
        <property type="term" value="F:single-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006287">
        <property type="term" value="P:base-excision repair, gap-filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045004">
        <property type="term" value="P:DNA replication proofreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05779">
        <property type="entry name" value="DNA_polB_epsilon_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05535">
        <property type="entry name" value="POLBc_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000002">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000010">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1600.10:FF:000006">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013697">
        <property type="entry name" value="DNA_pol_e_suA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029703">
        <property type="entry name" value="POL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055191">
        <property type="entry name" value="POL2_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054475">
        <property type="entry name" value="Znf-DPOE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10670">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10670:SF0">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08490">
        <property type="entry name" value="DUF1744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22634">
        <property type="entry name" value="POL2_thumb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22912">
        <property type="entry name" value="zf-DPOE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01159">
        <property type="entry name" value="DUF1744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 17" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 17" id="KW-0235">DNA replication</keyword>
    <keyword evidence="14 17" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 17" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="12 17" id="KW-0408">Iron</keyword>
    <keyword evidence="13 17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 17" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 17" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase epsilon catalytic subunit A C-terminal" evidence="18">
        <location>
            <begin position="1506"/>
            <end position="1885"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU365029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM01159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OEJ86014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="2203" mass="254486" checksum="DF273540F9940AAE" modified="2017-01-18" version="1">MANVRFKKQGNNYANNGFYNNNNGLSMMAQHMQQAAKTDEIDIMMGFERFIPAELDDSSAAAQLNNRRTGWLANMHPTLIPNTDEQSSGSLSYSGVSGVDFYFLDEEGGYFKSTIKYDPYFLVMVKNEKRVNDVEEFLKKFLESCLHSTEIIEKDDLKQSNHLIGLKRTLVKLNFINTNDLFEARRLLRPILTQNNNTQLTRDIYKDHHKANKSTTDEDVRLLIQDIREYDVPYHVRVSIDKGIRVGKWYTVTKNGFELFAERESFPDPVVMAFDIETTKAPLKFPDASIDQIMMISYMIDGEGFLITNREIISSDIEDFEYTPKPEYVGFFTIFNEPDELHLLKKFFEHIKDVRPTVISTFNGDFFDWPFVDNRCKIHGLDMYDEIGFAPDAEGEYKSSYCTHMDCFRWVKRDSYLPQGSQGLKAVTKVKLGYNPIEVDPELMVPYAYEKPQQLSEYSVSDAVATYYLYMKYVHPFIFSLCTIIPLNPDEVLRKGTGTLCEMLLMVQAYEHGILLPNKHTDPIERFYDGHLLESETYVGGHVESLEAGVFRSDLDSTFNIDTTAIDELLEELPSALKFSIEVESHAKIEDVTNFDEIKSKITTELLELKAKNKRSEKPLIYHVDVASMYPNIMITNRLQPDSMKSERDCASCDFNRPGKQCDRRLDWAWRGEFLPAKMDEYGMVKRALQNENFPNKNKKAPNKFLTFDELNYADQVAHIKKRLGDYSKNVYHRTKVSETVTREAIVCQRENPFYANTVRSFRDRRYDYKGRAKDWKKKLSQIPSSDKHAKDEAKKMVVLNDSLQLAHKVILNSFYGYVMRKGSRWYSMEMAGITCLTGATIIQMARKLVERIGRPLELDTDGIWCIIPKSFPEDYKFKLKNGKTLFMSYPCSMLNYRVHQHFTNDQYQTLDDPIRKKYTIASENSIFFEVDGPYKAMILPTSKEEGKGIKKRYAVFNDDGSLAELKGFELKRRGELQLIKNFQNDIFKMFLEGATLEECYGAVAQVADRWLDILYSKGAMLEDEDLIELICENRSMSKTLKEYEGQKSTSITTAKRLGEFLGEDMVKDKGLQCKYIISSKPRGAPVTERAVPVAIFSSDLEIKRAFLRKWLMDFSLEDFDPRAIIDWEYYKERLGSTIQKIITMPAALQDIENPVPRVEHPDWLKKKIATKKDRFKQSSLNRFFTKIENSKEKVLVNDIEDMFAEKPVSQVTKTSQIVSKRKRHEQNTEEEEPVLVLPEKCPDPDFDYLAFLKYSKIRWKQQELERKRRETIFGSKSGSLGRSALGGFLKKHAESYAKATWEVLQYKSSKDLDITDVHLRINDQIHTIKIKTPKIVYCTFKKGFQPDTAQFSDFVIEKSVTKHFQGGSTVGSQNVYKITLPHHLFDLELKNPCSLFNNPALLGIFEANIPSTERAVMSLGSTIEFNSKVKGALGIGLEKGFNITDLKAVESDRYLQRFDMMTCYLLHIVTNIGYEFFLLHKSWDETCEIFVLKPTSSAQELNHKTLINMFKSQYQKKMDKVRKHEKFVNISDHTEFAITHETDIKKLYRKLEKTLSQAKDEHGSKLVLLLQSPYVTNMLLSVNVLNSIPLIHFSMAELKLPQINWANVVCKRFVNHLLSLGSWISNLLMLSKYSNIPIGNMNLDNLGYMLDVLYARKLTVQNIGLWWNEKQPLPDYGGQEKNLDITSFFQNSIKAINNPEIYDSVVLEVEINNLMINTVLASTIIHELEGSDLADVDGGSLIEDGFNPGALAILRQLMKELWDSVMTTNNITAESLLQTFVLWVQNPESKLYDHTLNYHVQNLANKGLLRLVKEFGSLGSTCVFADKNKILLKTSKLTVENAYAYGQYIIKAVRTDPLFTYLNLNIKNYWDILIWMDQYNYGGRAATNIREDKEEQELWVFGNWNIKKYLAPIYRPEFEDWLVIILDSLIKTKVQKLVDLSDGTQRITQFVPVNGNGNTNHVAEKETVKVETTNDFIEGFTTSFHVPLLKRLKKLYANQQEYILDPAFADDYAIPRLPGVLKSPSVTNNPLLELAKSLFHILLLSNNRNIEVRKLRKEVLKLFQVREFDPKAEFQIASVQVSICNFTCEYCFYQTKLELCKLLDPAVLNCDGCHKKYNLFLMQEELIQMVEVELQNFYTQDLKCSKCFKIKEDLMSEYCNCSGKWVETVPHHTQKLKLNVFKQIAAFYEFDLLKTLLNDLHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGN3</accession>
    <name>A0A1E5RGN3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1572</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGN3"/>
    <dbReference type="STRING" id="56408.A0A1E5RGN3"/>
    <dbReference type="InParanoid" id="A0A1E5RGN3"/>
    <dbReference type="OrthoDB" id="436519at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051948">
        <property type="entry name" value="Hsp70_co-chaperone_J-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="7"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ86016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="267" mass="30247" checksum="C08A3D2D00443326" modified="2017-01-18" version="1">MLAEEIDLYKVLHSTIDDEPSQLKAQYMQLVLAYHPDKLPPESSTSERKESEEKFHLLQKAYGVLQDQASKRVYDNWYRKNSEKIGINSNSNSNSFARTTNRQIKLQEYGVYLRKLQHFDVPLENAVSFEKFSSANAKFKNSGSEASFSKRNQPHESAKSCLNDSNLVESKQLYVRVDTGVAESLLSQANNTDDIELIKNNFTNIATIEPLSGKDFSSTHEYIVEFHTFLDTLHNLNTASENSSCANLVVKPKCFVNGTYHFGLSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHE8</accession>
    <name>A0A1E5RHE8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1544</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHE8"/>
    <dbReference type="InParanoid" id="A0A1E5RHE8"/>
    <dbReference type="OrthoDB" id="3163292at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="1">
        <location>
            <begin position="22"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="847" mass="97109" checksum="1271D5D7F76E4B77" modified="2017-01-18" version="1">MSSNNTNQPARDSPDSVKTFRSCDRCRKSKIKCDSTDRYPESCTHCLHKNVKCEISVMGKPFRCKPTTKDFHTLYNSINDLNDLMTCLQEQNGDFPTSKHEDITQKHGGNKILALPVFDSTTLLTKEIVINLRYDNEMFYINNYSINTAELDKIMHLYNTTLISDYINIYKTWHHDPLFGDSEHAVSDLHVDWRKLFTNNELLQLLIILNFYIDIPFVDFQFQDLLNTLITLYTGQDKSLEKGSSSLFAQYVPYIIDAEHFDISKKLSSNLTSDYFIKNFTKILLTILSLNGPTVNDNLYSVAMKLLHLKKIDYIKHWDLKFYKFIVSTVFAHCRKTNCAEKEELDNILNFLQNGGWKNTQSSESRGHTFQYLTRYDEKLVKRDTVSLESEAMFEKSFVEVYTSLKSSTTIFTLSTQLRLSYVQMFLCQLVSLNLIYYTNKTGDFTYMDDFKMSKKGTLLFNGWNYETPKNESSYHHSQYQINAIHQKYEKETTTKKLLNLMTLPICKNHEPALEVKDLGLLDVVSWELHTGTNIMKSADFFIFHLYKEIIQRQMLETLNISESQNNETENFTTQSHLHLQNGLQLSFLSDPGNNELGKKFQVVLQKAVPVSLDKRVSNTDDSIDLDMCSPNIAFLSQTTDPDVTKEIEDILQISEAVPMQLSESLTSTSSSVFSGVQDSSSSRKTSVESVNSVLGLEPKEVVSNSFTEEWGYDLVHPKEQHQKQTLYDGYSSAALQTTTITTSIAQDGASFSKTKYVAIEPKFCNPAMDLDMSSMVESFKPQASKLKNNKIVKKHSKRKHQETLSAQKSLAHNNGRLRINNVLEDVDWIKDSTEDVLQKIHGVLEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHI4</accession>
    <name>A0A1E5RHI4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1478</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHI4">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHI4"/>
    <dbReference type="OrthoDB" id="4040439at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="102" mass="11756" checksum="0C4856E009C6EF3C" modified="2017-01-18" version="1">MNNDSPRNKEELYKQLASQCRMLNENLYITKLELNKLTKVSRENVINQIGIINGSLLMGANKYFETQLTRRADKLEEETAEHQQGSEGMSDARAEQQEQVQX</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHK5</accession>
    <name>A0A1E5RHK5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alkyl transferase</fullName>
            <ecNumber evidence="2">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1457</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHK5">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHK5"/>
    <dbReference type="InParanoid" id="A0A1E5RHK5"/>
    <dbReference type="OrthoDB" id="4173905at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904423">
        <property type="term" value="C:dehydrodolichyl diphosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016094">
        <property type="term" value="P:polyprenol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291:SF2">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT SRT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="393" mass="45460" checksum="2758B05A6A889BA2" modified="2017-01-18" version="1">MLRKLVFFRYNHXKEIYSKKYANTAFIQKINAKSCKSNCLKLLYMAMAFPVISQIMSAFQLLLLLILSQGSVPQHLSFIMDGNRRYAKGIDHPAKTGHEVGSVTLVEMIYYCRMLGVQSLSVYAFSIENFNRSQREVSLLMDLLVAKLTEFNERSKSPLLQDKFFQNCQIKVVGDKSYFDADLRARLETCENGSDSFDADSIHKKPHFIIYICCPYTSKNDIYHAMVKNIKAQPEMDANLEQITKESVTNAMFLGNSSNKCDLLIRTSGQTRFSDYMTWQVHENGSVVFTNTLWPNFGFFELFAIILKWSYYKXYEVWNAKKPXGTFHMPLHLQKNVHQSHPFTHACLQKVFSLNXMMNLPQLKKSSIWQKRETSIEDLPEPPISLSVXDRNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHL8</accession>
    <name>A0A1E5RHL8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Mitochondrial escape protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g1010</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">Plays a role in maintaining the mitochondrial genome and in controlling the mtDNA escape. Involved in the regulation of mtDNA nucleotide structure and number. May have a dispensable role in early maturation of pre-rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion inner membrane</location>
            <topology evidence="1 11">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the YME2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHL8"/>
    <dbReference type="FunCoup" id="A0A1E5RHL8">
        <property type="interactions" value="149"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHL8"/>
    <dbReference type="InParanoid" id="A0A1E5RHL8"/>
    <dbReference type="OrthoDB" id="10267654at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018850">
        <property type="entry name" value="Mt_escape_2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039627">
        <property type="entry name" value="Yme2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32198">
        <property type="entry name" value="MITOCHONDRIAL ESCAPE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32198:SF2">
        <property type="entry name" value="MITOCHONDRIAL ESCAPE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10443">
        <property type="entry name" value="RNA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Mitochondrial escape protein 2 C-terminal" evidence="13">
        <location>
            <begin position="313"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="680"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF10443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ86375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="735" mass="84055" checksum="1933A045D2F57105" modified="2017-01-18" version="1">MFSQRVLLNSFKFHFRKTPYNKVWNANFKRLISTDIKKADALLGETNSTVIEKSKEETLLYFDNVYPRANLFSWYDFLSFKSTDASVRDRIVSYAGNIEMTEMIPMKRDGGCFAKFSVPPDAKISDINATIMANTREKNKRKIFSQVSCFPTKGIPLIEDMQRLPSKTLVVKYDGFLKEEDLYSLFRRYGQIVDIIPETNQSTIKFKSLKGSVCAKNCVNGFTISGCTLNIHFAKNSSSHVIQNFFFNHTRLAIPFVFALISIIALLIFDPIRELMVELKISKKYEFYYSYYNALVSFFSSNSEKIDDGLYHSEIEDFQLWLEENNNTFVVIRGPRGSGKHNLVRHALADRKNVLELNCDSLVKTRIDSRFLRNASSQLGYYPIFPWLNSFVGIIDLGVQSLTGAKSGLSESNEKQFNTMLATAMISIKRLALSDFQSGNEEDYLQQHPEAKPVIVIDRFNNKSDINSFVYKELAEWAALLVQMNIAHVVFLTEAVSPVKLLTDALPNQVFKNMVLSDASNEISRKYVYSNLNTEIDSKCIEPLGGRMLDLQGFVRRVKSGEHPEEALEKMIIQGTEQITQMFLSNPSIRSAQAWELIELLSDAGSLNFKDIVFRPIFKGDPETALLELERDGLITISRDRGMLDVIKPAKPLFQYSFKNLVSDEKSSRILKTSYYLQIIAFETKRILKWEEELKAFKEQNEKLFKSRLTYLSNKIQLSSDVIDNAEASIKKLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHY9</accession>
    <name>A0A1E5RHY9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">LAS seventeen-binding protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1626</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHY9"/>
    <dbReference type="FunCoup" id="A0A1E5RHY9">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHY9"/>
    <dbReference type="InParanoid" id="A0A1E5RHY9"/>
    <dbReference type="OrthoDB" id="10068368at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14232">
        <property type="entry name" value="GAT_LSB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044103">
        <property type="entry name" value="GAT_LSB5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038425">
        <property type="entry name" value="GAT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045007">
        <property type="entry name" value="LSB5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47789">
        <property type="entry name" value="LAS SEVENTEEN-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47789:SF1">
        <property type="entry name" value="LAS SEVENTEEN-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="386" mass="43999" checksum="E42D0B16A8A70C6F" modified="2017-01-18" version="1">MGFLKDHQHTRITDVVVKTVSSDRVSVTTVLPTLTSLIEKGVDDDEYDDEDSRRYLDYDVDFMGSRGSRKQRNRRSHTASQLTDYEYVQNQLECGRQIRKFFKYGKLPEKLKSLQLFNLIVSQDLHYPEMFNEPKLLKQLECLALGVNSGGSGSVPEYMDMKNVKKLMKITKTYITSWCSGNRDAKMFHGCYAGFFNLLAKLYKSPSALSGGKSKKRQVSRNFMMDTADSSLHPDDVKYGIPVIIDIKKETPRIKILISDSMNCAVSLKNSLSQLKKNERSVDNEQCTSWFIKTRDIRRKVLRYLQLIPDGEFLGALISTNDELVAALQKYDNLSGQSADYSDLDENSELSDLSEEISESDPEEEEEEEEEYGADSNNPFGDSNHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI75</accession>
    <name>A0A1E5RI75_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">cystathionine gamma-synthase</fullName>
            <ecNumber evidence="9">2.5.1.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">O-succinylhomoserine (thiol)-lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1180</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the formation of L-cystathionine from O-succinyl-L-homoserine (OSHS) and L-cysteine, via a gamma-replacement reaction. In the absence of thiol, catalyzes gamma-elimination to form 2-oxobutanoate, succinate and ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-succinyl-L-homoserine + L-cysteine = L,L-cystathionine + succinate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20397"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57661"/>
            <dbReference type="ChEBI" id="CHEBI:58161"/>
            <dbReference type="EC" id="2.5.1.48"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-cystathionine from O-succinyl-L-homoserine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the trans-sulfuration enzymes family. MET7 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.48" evidence="9"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RI75">
        <property type="interactions" value="198"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI75"/>
    <dbReference type="InParanoid" id="A0A1E5RI75"/>
    <dbReference type="OrthoDB" id="10047078at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003962">
        <property type="term" value="F:cystathionine gamma-synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000094">
        <property type="entry name" value="Probable cystathionine gamma-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000063">
        <property type="entry name" value="Probable cystathionine gamma-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054542">
        <property type="entry name" value="Cys_met_metab_PP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051750">
        <property type="entry name" value="Trans-sulfuration_enzymes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42699">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42699:SF1">
        <property type="entry name" value="CYSTATHIONINE GAMMA-SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="269"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="344"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="269"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="344"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="364"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="376"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00051441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ86600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="768" mass="85919" checksum="BE694DA3DBEAFEC2" modified="2017-01-18" version="1">MNNFINISTQVGESIPPQTEHAVSVSLPTWEANVGYEEGDPNIINKLTTGYPRFFIHLYIKKLCSLILTKFSRNDSVEQCLIFSSYNVAKRCREYIKVKRPSTSTYPIRILQLSSEKPNSLDESRYKQECKVSTVFVHKDDFKYLKEYWQHTGEGISSRVAEYMLRNLFDIDDQTLQSISHEHALQTSTETPISSTNITITEKEKEGTTTSTSYIEQRFGRAMDMSMGNYAKRLIKKRIALKVVDVEEETVXXNXSTATTIIAVSNEGRNTVTTPSQPMEVQNHGAQSPSSNTAAQDTHVEDISAQFTEHVIVVETNSTEQNPNMTTSNLQRNRALESEFGNHVGTTQSSFANGTHSREGTPGLNDSGSANERFNTHNPSAAAASNSRTSTTFVDPNKDVYLFPSGMAAIFTAHRLLLEMDSLRVKSRMSASSLASSSPMASSLVGYGPPYKKTVMFGFPYTDTLSVLRKFNHTHFLPNETDAMSKLETILQTGEQILAVFTETPSNPLLKMGDLLKLKDLSLKYGFYIIVDDTIGGFINVDILPHADIVVSSLTKIFSGDSNVLAGSLVLNKHSRLYDFAVNQFFPKSYEDLLWCEDAICLERNSRNFVQRSLKVNTTTEWLIDTILKPYEDXKSTASTIEPGXSLNHKANLFAKIYYPKYTSLETKENYEKVMTSNGGYGEVFSLVFHNLSQAKAFFNNLKLCKGPSLGTNFTLSCPYTIIAHYNELEEVAKYDVAKSLVRVSVGLEDQAYLEKVFLFAIEQALKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI79</accession>
    <name>A0A1E5RI79_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1070</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RI79">
        <property type="interactions" value="257"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI79"/>
    <dbReference type="InParanoid" id="A0A1E5RI79"/>
    <dbReference type="OrthoDB" id="6159137at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052462">
        <property type="entry name" value="SLIRP/GR-RBP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48027">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN 87F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="206"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="306"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="660"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="485"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="605"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="696"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="485"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="696"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="706"/>
            <end position="716"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ86293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="977" mass="110144" checksum="5447B801A99BFCF2" modified="2017-01-18" version="1">MPKPNQNYTKHSAESVNHADTRNENSIEPHQLEHSHQDQIPSLIMSQLELLANTNCLTIRLKAIANSDNTMSKSLKELYTSIVDNLIFFINSKSSKEHVRKIDLIENYQFWPTSKRLKHIQDNRTSLIFELGDELCLCENEHEMIPFETDDKSTFDNILQYKCNDLAMLSGXMELVAGFLKDMTAKHPNVFLYNISLNKYSLSSPGNLYIKGLPKSQNVESLLPYFEKYGKILNFKIILSNETNRSLGYGFLNYQFGSQASECIKKLNGTLLNNSRLFINYHVERKERERLQISQIKEGNDDNKFRGVFIGNIPVFDKNSQKFLSPSNVVEKILEILIDKNSLPNIKIVSFYFPKDNSQTNIQYNNEIDEVLDNLETCVNQHEESPLKGYGFIKFKQHDQALAAIKXCNNFNWLGHKLVVNKAIQNKAPEFHLTEYQSPVHSRTLSVSSNINDFDSSANISQMGSPLTNSPPSTVFSALNTNKTSTRTSVDSSNHYMKHSDSVGKTSNGHHKDTQKRRFSVPYFVHQNGPIVPHQSMIPNFYPNNMVPPPPFGQFPPGPVPGAHTFTPGTPPVFSANGSPPLGNPFNLCSSPTMHPLFYNGTNQAPMSEGFSKSRKNSGDPKHHKKYKNPYFHGGQNAHYQAPGPLFYGYPIPTNNQQESNLYVKNLPLTWKDDDLSSFYQNYGEIISAKVITVGGSTRSSSDASETYKDSDKINDDDSLERLSPQNTRKFESVEESTSGLVEPHTSRDDEVANMQAQELEMGMSKGYGFVCFKNPLDASKAMFETNGMEVDKNTITENNKQSVISNVLQVSFAAKRHKSTSSSAFDIQCNQRPYKNNDQNYNSNNPANPYAPNSFNIKFMQALHEQQQALFCSPVYIPSPPLSQGMGNGYSQLLPPPPPPPPSLFQQNRYHRNSHPVMNNTGRQNPVQSPSPTMKFQPEIFTRPSSESRTMGFAPILDDRIQLPMNTTANQADNSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIC6</accession>
    <name>A0A1E5RIC6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="7">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1164</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RIC6">
        <property type="interactions" value="517"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RIC6"/>
    <dbReference type="InParanoid" id="A0A1E5RIC6"/>
    <dbReference type="OrthoDB" id="8118055at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044322">
        <property type="term" value="C:endoplasmic reticulum quality control compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904380">
        <property type="term" value="P:endoplasmic reticulum mannose trimming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044674">
        <property type="entry name" value="EDEM1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679:SF5">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184782" description="alpha-1,2-Mannosidase" evidence="8">
        <location>
            <begin position="23"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="377"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="424"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="509"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="842" mass="97684" checksum="D8184979D64081EC" modified="2017-01-18" version="1">MNTLLIRHLACLTLFFPHLVASYHEYSFSKHDLQKYSNQTKDLFYWSLDQYFEHAYPFDELNPIDCTAKTRNFADPQDTVTNDVLGNFSLSLIDSLTTLIVFQDVQNFTKIVELLTNDLDPHWQIDSTIQSFEAIIRIIGSFLSAHIHIVDQNFWDSSSKTTPQYKDVKFNLLYRAEKLADIILETYKTPSGLPLPRVNLKNGLQGIAEFDSSNNPVAMGCPFLEFHLLSILTSNDKYKIVTQYAYDRVIHELTSELNLIPHSVDPVSLQTLEPYTNVGASIDSIYEYSLKNAILFNNETTMQVYRDLIKSINVYSEEFWFYKTVDIHNMGSSIAFWIDSLSAFFPGVLVLNGDVDNATYKNMFYWKLWNYYGGIPERWNFQSAGNSQIPPEVEINNQXDLERAMKRQRIDSIVNLEWYPLRPEFIESTYYLYQATRDPMYLNIGKQVLHDLQTRFKCKCGLCGIQNIKTGEMQKRTESFVFSETLKYLFLLFNHELQPKRFDNVIFNTEAHPLYVPKKMYQXYTKTRFFNDTYFSNLQDYWDLWKESTKSEYNLFVNNNGGNATCPVSEKFMILPFLQNNKNXKTFMNSPILNDREKLFEFDRQYHTQLVYPDWLIEQRGNDTHFQMEVNKEFIDVYSPSSILQCKLSKEAAITGDSFALEKQYMENPAGAXTGEMPSFGQTLKLVLGTNRQYAVENNQLGSNFFKSLIGCNLYIITEGGSHKARMHGDAMEMGNSDTKMYAFLTRVDDQHFEPDQXIYVNKTLLMPSTKXEFEYDEPYYEKTANKLHGEGSXGFFXRSSKKGYLKTVLTNIKDIQKIISVNGNGQVXVNNGIFLQNVYAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIR4</accession>
    <name>A0A1E5RIR4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DOA4-independent degradation protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1382</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RIR4">
        <property type="interactions" value="739"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RIR4"/>
    <dbReference type="InParanoid" id="A0A1E5RIR4"/>
    <dbReference type="OrthoDB" id="10252926at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10476">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="15"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="224" mass="25813" checksum="2D282995D91C9875" modified="2017-01-18" version="1">MAILDWVFGKQMTPQEKLRKNQRSLQRAQRELEREKHKLEGQERKLVQDIKKSAKNGQIKAAKLQAKDLVRTKKYIEKFDMMATRLQAISLRIQTVRSSDQMAQSMREATGLLGMLNNSLNLPQLQQITMEFEKQNDMMDQRQEMVDEAIDDAMGXDEFEDEEEADEIVNKVLDEIGVDLNTKLNDVPSQNLEAPETLSAIAEPQKVPQSMDDQLQARLDSLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN12</accession>
    <name>A0A1E5RN12_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative glutamine amidotransferase DUG3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g626</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN12"/>
    <dbReference type="FunCoup" id="A0A1E5RN12">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN12"/>
    <dbReference type="InParanoid" id="A0A1E5RN12"/>
    <dbReference type="OrthoDB" id="14446at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061672">
        <property type="term" value="C:glutathione hydrolase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008242">
        <property type="term" value="F:omega peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01908">
        <property type="entry name" value="YafJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000045">
        <property type="entry name" value="Glutamine amidotransferase DUG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026869">
        <property type="entry name" value="EgtC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052373">
        <property type="entry name" value="Gamma-glu_amide_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43187">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE DUG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43187:SF1">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE DUG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13230">
        <property type="entry name" value="GATase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="2">
        <location>
            <begin position="2"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="358" mass="41059" checksum="2CDC42F39A6E42FD" modified="2017-01-18" version="1">MCRFLIFKGKEPIKLSHLLTRPAHSIINQSFDSRLRLDRRRPINGDGFGVAYYPTDRDLEQDGPCLFKAITPAWNNQNLETLAEKTKSSLVFGHVRASTYGVLSETNCHPFTYHSLTFMHNGGIANFKKIKRRFLSELDDEYFNYIQGSTDSECAFALFLDILHKMGHDPSKKEGNFGDRVLRKALKKTIQKIKYWTQEANKLVKEDEKEEPSLLNFAVTDGDSVVISRYITSKTDEAASLYFSCGSSFKEYAPREYRMERLDRNSDVIMVSSEPITFERCDWTAMNTNSLLTIKNQTILLHPIIDEYYQIDPLYTRDSEIAASKGLMGSIPVAIEQERFVPPLEREGRLSRPVTHAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN26</accession>
    <name>A0A1E5RN26_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribonuclease</fullName>
            <ecNumber evidence="9">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g791</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase HII family. Eukaryotic subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="9"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN26"/>
    <dbReference type="FunCoup" id="A0A1E5RN26">
        <property type="interactions" value="437"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN26"/>
    <dbReference type="InParanoid" id="A0A1E5RN26"/>
    <dbReference type="OrthoDB" id="7462577at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032299">
        <property type="term" value="C:ribonuclease H2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07181">
        <property type="entry name" value="RNase_HII_eukaryota_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.460:FF:000001">
        <property type="entry name" value="Ribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000016">
        <property type="entry name" value="Ribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.460">
        <property type="entry name" value="Ribonuclease hii. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004649">
        <property type="entry name" value="RNase_H2_suA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001352">
        <property type="entry name" value="RNase_HII/HIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024567">
        <property type="entry name" value="RNase_HII/HIII_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023160">
        <property type="entry name" value="RNase_HII_hlx-loop-hlx_cap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00729">
        <property type="entry name" value="ribonuclease HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954:SF7">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01351">
        <property type="entry name" value="RNase_HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51975">
        <property type="entry name" value="RNASE_H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-2" evidence="10">
        <location>
            <begin position="33"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ88290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="327" mass="36836" checksum="D1E8C75D21739DEC" modified="2017-01-18" version="1">MLPPSVQSALQSTHTTTFVSDLPQEIQNHKDQPVIIGVDEAGRGPCLGALVYGISYCLKSFEQDLKKNYVFDDSKKLTDAVRKSLFSQIWDISNSEVSKLSLELSQNVGYATTAIQATDISSGMLKFPPTENYNLNEQSHDATINLIHALVNKGLNIDHIYIDTVGPPHTYQKKLETIFPRIKFTVAKKADSLFVIVSVASVVAKVTRDLVLEQMISKIYHPQSMAETQDTDHKTEPVNCGSGYPSDPNTVRWLKSNQTPLFGWPKEITRFSWSTCQTLFKNPQTAIIIDWEEDFIVNGTNNNKKAAQLFMLNNKQEKLVTLDNWYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN69</accession>
    <name>A0A1E5RN69_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protease B inhibitor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g878</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Cytosolic inhibitor of vacuolar proteinase B (yscB), probably regulating protease B activity during limited proteolysis. PBI2 is a component of the LMA1 complex, which is involved in the facilitation of vesicle fusion such as homotypic vacuole and ER-derived COPII vesicle fusion with the Golgi.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the heterodimeric LMA1 complex together with the thioredoxin II/TRX2. LMA1 binds to the ATPase SEC18.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protease inhibitor I9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN69"/>
    <dbReference type="FunCoup" id="A0A1E5RN69">
        <property type="interactions" value="184"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RN69"/>
    <dbReference type="OrthoDB" id="5518345at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000005">
        <property type="entry name" value="Proteinase inhibitor I2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052471">
        <property type="entry name" value="PBI_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28288">
        <property type="entry name" value="PROTEASE B INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28288:SF2">
        <property type="entry name" value="PROTEASE B INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="1" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0722">Serine protease inhibitor</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00062658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ88314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="73" mass="8185" checksum="9CCBD63DC61C77F1" modified="2017-01-18" version="1">MSEKSFIVTLKESADPEKFKESVNKLGGSISHEFNLIKGFTVKLPESLHIDSLTKKHESEIATVEEDSKVHTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNB6</accession>
    <name>A0A1E5RNB6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA-directed RNA polymerase I subunit RPA34</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g989</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNB6"/>
    <dbReference type="FunCoup" id="A0A1E5RNB6">
        <property type="interactions" value="236"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNB6"/>
    <dbReference type="InParanoid" id="A0A1E5RNB6"/>
    <dbReference type="OrthoDB" id="4089784at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.250.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013240">
        <property type="entry name" value="DNA-dir_RNA_pol1_su_RPA34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053263">
        <property type="entry name" value="Euk_RPA34_RNAP_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28155">
        <property type="entry name" value="ACR243WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28155:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA34.5-DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08208">
        <property type="entry name" value="RNA_polI_A34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="239" mass="27253" checksum="D7E2AE1B5C0EDD33" modified="2017-01-18" version="1">MAKLHTAASTASKPYISDSDESDLELEKDYLSTAPSSNYKEFQNSKHFHKLSDSSLKKKQLILIKAPKDFDFSKMESIPLDNNKGGDFSTVLDDVKESKRSLGKQYYIKKDTLVNTRNIKVMSLSKDKYLKIKGDLQIKDVYSINSGVEIPKIQLDQVKTPREDVVKVEGLKMEHFATGYDASDFAEANVSSSIKTSSKKENKPKKVDESSSRKDKKDKKDKKDKKDRKEKKEKKDKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNF2</accession>
    <name>A0A1E5RNF2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DMAP1-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g685</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RNF2">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNF2"/>
    <dbReference type="InParanoid" id="A0A1E5RNF2"/>
    <dbReference type="OrthoDB" id="69964at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010506">
        <property type="entry name" value="DMAP1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056881">
        <property type="entry name" value="Mug62_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22754:SF32">
        <property type="entry name" value="DISCO-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22754">
        <property type="entry name" value="DISCO-INTERACTING PROTEIN 2 DIP2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23024">
        <property type="entry name" value="AMP-dom_DIP2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06464">
        <property type="entry name" value="DMAP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24919">
        <property type="entry name" value="Mug62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51912">
        <property type="entry name" value="DMAP1_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DMAP1-binding" evidence="2">
        <location>
            <begin position="5"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1612" mass="182845" checksum="CE7F3FB8A8F03C17" modified="2017-01-18" version="1">MDFSIPEYLPVEAQRRLQELVQDFQEENLTQKGYTNKRAQLIDKYTSYNGGRNESVSSDRYEPRISIDSDNKSTSTHNNVTRKIDSNHLQVYQTPDSHSLHKLSSASTNGTPVSALQNTRHQEIMPQSAFNTPQSNNGFVDTSTTPSSKVSLVKNNSLSQRASINRHNTSLHHRKKSSQISLPFDLIANQYDPMTPILPRVETLTPTFKGASLTYILQARSVIQQKETAIITINVKGKEKLLSWSKLHGRSERIAQELTKNKKKIHKMAKILLWFDQDDLLEFVVSLFGCFSADMVAVPVSFDTYSLNEIIEIIKLTNSKNILISDDCYKNLENLTTNNGKLKLLKSEFFQDINFIKTNDLGTYTKAKNINNIPNISYIEFTRTPLGKLSGVLMKHKTLENQFELFAQVLNSRNPKTKVIRPIKLSPGKTTASMATRYTVINSLDSTRNTGLIFGLLFNIFSGNLMIHINSKYLSMSSGNLETCVDKYRANVLLCDQLQLKQVVINYLDNPELMQKKNKRKIDFSCIKFCLTTCTTIDTEVSDMIVQKWLKNLGCIDASQCYSPVLTLPDFGGCFLSFRDQLSIPPKTLKKVHGQVQLITDDNFSAEYDDSAESTGNGSLIKFKNNTQDDIYIEKEPLKDNVIKISSTPMVGASSSAQKHIKLSAFGFPLVDSFLAVVNPDDGTLVQDSTVGELWISSSSMTNEFYHMDKVNDFVFKAKLNYNKMFRMLEKSNAMQQSFQKLKAIMQICPSETSFLRTKLIGFVHNNKIYVLSLVEDMVLQNKLIRLSNGLHTCDLTSSSVTSTSSDAASSLSLSANLHNRDPRLLKKRVVQTHYLQHITENIVRTVEHVREISAFELPQYKLEHFLVICIESDLVKSSSEPSSSSDKVLNSFVDKIYKILWIFHRIQPMCIMVLPVGSLPKRYCSLEIANSTVEKKYFSAQLNVKFVKFQLDNVILDFIPRTSCENESVFSEHLSNYRHASIEDVILRQGHTQANFTQFFQTTNNNIVESSVDTRTHILLNKYKSIMEILEFRCGQQGNDLAYSDGYATAPPKNSNNYHKSITWSSFNNMVASYLKKIVESKTPLKSGDSIIVVSENSVEYAAMVITAFYCGFNIIPMPSFNQSSLTPQKDTEFFAKVVKAYKVKRIFCDFLNQTYIEDSNTVTGKFFKKLKHMLPRVTIFSKVKVKSQHNIKFFKSQIKSKLGTVREASVVFVDKSDDMSSKLVTTMSASTLLNLCKQYKNVLHLSSDFRLLSACSHTDQIGFVRSCLLGIYVGTTTTLYSVSDIKHDPKEFFMDMKNLTIKDLFLSKELFTFILMKAKENVLRGIPHILLPVKGRPDHYFNLKLVLKYPQAGCNMGNLNYIYENKFNPFISLRSYPPISIFLDCESLRVGIIKEVDPHEEPNCIEIHDSGVPVCEELRIMNPENGQNTYINELGEIFVSSSKSVDNYCLIDQSGKLVRDKFLTKQLMTDVGGKTFMRTGDLGFVKPFMIDNKQVNILFVLSEINNTIESSGFHCAVDLEKTVETIHACIEKCRVAKMGGYTIALVELRKELKNQKGNLVPIIASNILQKHKLIINLVCFVSRIKDSRSQTINDFVKNNENYIYGVYRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNM3</accession>
    <name>A0A1E5RNM3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Malate dehydrogenase</fullName>
            <ecNumber evidence="3 12">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g959</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="3 12"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNM3"/>
    <dbReference type="STRING" id="56408.A0A1E5RNM3"/>
    <dbReference type="InParanoid" id="A0A1E5RNM3"/>
    <dbReference type="OrthoDB" id="4069699at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070013">
        <property type="term" value="C:intracellular organelle lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000268">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.110.10:FF:000009">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001252">
        <property type="entry name" value="Malate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010097">
        <property type="entry name" value="Malate_DH_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01772">
        <property type="entry name" value="MDH_euk_gproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540">
        <property type="entry name" value="MALATE AND LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540:SF72">
        <property type="entry name" value="MALATE DEHYDROGENASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00068">
        <property type="entry name" value="MDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 12" id="KW-0520">NAD</keyword>
    <keyword evidence="5 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="14">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="15">
        <location>
            <begin position="160"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="184"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="7"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="127"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ88464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="386" mass="41978" checksum="9F109902BA7B00E6" modified="2017-01-18" version="1">MKVTVIGASGGIGQPLSLLLTQNTTLPINELALFDLRLSKGVSTDLSHMTGPTTGIDAISPKKVKGYGKDELSGALENADIVVIVAGIPRKPGMTRDDLFKINGGIMRNLILETMKHARPDCLVAIISNPVNSLVPLAVETMKQDIHQKYAPEKRVFGVTTLDLVRAITFLKESGQENVTQNGNSSNCNNNSNSNSSSKEDIFKDIVVIGGHSGKTILPIIPESTLLKKGLAKGTNTKDYNDFVYKVQFAGDEVVKAKEGMGSATLSMAYSGYMFVSDLINARCNTQNKQAYTSSTTGMSNTIVAYVCLNNNNKGQQLKEKLHNSVDYFSTPVVLQKNTGFVEHLDFDHTLNKLNTHELQLLNKDVLPQLAKDINKGESFVRSSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNS9</accession>
    <name>A0A1E5RNS9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoinositide 5-phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g704</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptojanin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the central section; belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.36" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNS9"/>
    <dbReference type="FunCoup" id="A0A1E5RNS9">
        <property type="interactions" value="380"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNS9"/>
    <dbReference type="InParanoid" id="A0A1E5RNS9"/>
    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043813">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000029">
        <property type="entry name" value="Inositol polyphosphate 5-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF257">
        <property type="entry name" value="PHOSPHOINOSITIDE 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SAC" evidence="11">
        <location>
            <begin position="219"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="175"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="968"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1013"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="175"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="186"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1017"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1049"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1141"/>
            <end position="1151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ88373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1151" mass="130067" checksum="D4A7F6017D6F0C69" modified="2017-01-18" version="1">MEIYVQQHPDHRKIALCSSTHALIFQALNADGSNGRSRSDGPSCSMELKPKDAVTSNRIQEQNGTPSSSATGQQNDSSKFFQRLTFKDIHGFIGLIEVEKLIFIVCITGTTKVASPVPGETVNKIFSVEFFCLNDSRWDFIEIDSSGYPILDEHDQLNEKNGNYGDADWNSQTMGNINENYSYNPSRNGNSRYDSSRSRSSPPEQNMHPLMGKHPANDLKKLLSNGSFYYSSDFDLTSSLQKRGGLSNKHSLSLDQFQEEYMWNSFLMQEMVSYRDRLNDEDKQILDEQGFLTTVIRGFAETFVSYINELKCSLTVISKQSWKRAGTRYNARGMDDEGNVANFVETEFVMYSKEYCYAFVEVRGSVPVFWEQDTSLLNPRVSVTRSVEATQATFDTHFERLYSKYGPINIVNLLSKKSSEIELTRRYRAQYEHSKVKNEITMTEFDFHKETASEGFAASIKIKPLIENFLLNSGYYSYDVVAKKTMCEQYGVFRVNCLDCLDRTNLIQQFISNLAFKLFLKDFNLMKGMRASMFNDDTEVFVKHNSLWADNGDQISQIYTGTNALKSSFSRKGKMSFAGALSDATKSVSRIYINNFMDKDKQNNIDKLLGKLPDQIAVQLYDPMMDYVSAKMKLTLEQYSSSELINIFIGTYNVNGITRKADLTKWLFPIGDKFKPDIVVLGLQEVIELTAGSILNADYTKSSLWEKMVNDCLNQFGENDKYLMLRTEQMTSLLVLFFVRADKIDNVKQVEGGSKKTGFGGITGNKGAVAIRFNYGETSFCFLNSHLAAGATALEERKLDYENIIKGIKFNRGRTVSIHDNIFWLGDLNYRIELDNLEVRKMLSDPTSNNIQRLLENDQLNKETRIGRVFDGFKEPSIKFLPTYKYDLGTNMYDTSEKARTPSWTDRILYKGNDIRPLSYSDVPLTFSDHKPVYAAYRAKVEFVDPAKKELFIVNFQNEWKKSHHLKEVSVGEQISRPPSLDPAREPHRPQLPPKPRKTVQLHNDVSLLDLDLPAESSNDSSSTLSLGDSVASSRTSNVKSAPPLIPNKPSNLSLNTTHSAFTSERDIPVKPHSSVPPPLPAPRRKISNSSSTTMPPGFDQGLTLKSKDGTPKAESPLSPGTPALEKKIAPVKPVKKPDLSVNSWKPLTPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNV1</accession>
    <name>A0A1E5RNV1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">J protein JJJ1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g587</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNV1"/>
    <dbReference type="FunCoup" id="A0A1E5RNV1">
        <property type="interactions" value="909"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNV1"/>
    <dbReference type="InParanoid" id="A0A1E5RNV1"/>
    <dbReference type="OrthoDB" id="5894at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000046">
        <property type="entry name" value="dnaJ homolog subfamily C member 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051964">
        <property type="entry name" value="Chaperone_stress_response"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054076">
        <property type="entry name" value="ZUO1-like_ZHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44029">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44029:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21884">
        <property type="entry name" value="ZUO1-like_ZHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="6">
        <location>
            <begin position="10"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="380"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="439"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="491"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="598"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="700"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="493"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="598"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="614"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="629"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="712"/>
            <end position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ88562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="724" mass="83571" checksum="ED009B4C265A001A" modified="2017-01-18" version="1">MSRQNETKTCYYELLGVESTASSVEIKKAYRKQALVYHPDKNPDNIEEATLRFSEISIAYETLADPQERSWYDSHKNEVLYGGSGETGDAEDGTYEYYDSNDPIVTGITCDEIMKFFMPSLYTRLDDSPAGLYQIAGKVFSRIAKDEILFARRLGLESKIKFEDDFYEQDIRQDGYVETCKKYGNSNGISGSTLLWPSYGYSETSYEDLKKFYKKWGSFNTVKNFAWKDEYRIFRNYDRRTKRELNKRNERIRNQFKNEYNKTVKNYVNLMKKYDMRMKLGAKREEDAKRLQRLKDFEALKQKVQTKSSATENIQYEEQAWQAVDESMYKELEKTLADFDDSGNLSCDEKQGNEKTALHADASFQEDYSKEDEEEVVYVYECFVCNKTFKSDKQLTNHNNSKMHKQKLNQLQREMKQQDMELGLDQVSDFEDFESANEDDCEEGEGAVITKEDDATDVDQGNNDLLDEMENVQDLDALQAELDKLDAELEALGSEEDSEEELDADELDAGEERTANTFEFEPDFEIDTDIDHSDDSITIFEPQKIVSKNTNLDNTKSDSIQVTQASDQEDLQNNLDVDDLANLLSSLNDKKKPKKFNFAENESDWNGNGSTSNKKGKKSKKKKVKPRQESSSTTTDHTPISDAPPTKTQNFENVLSPETVCGKCGVVCETRNQLFKHVKLTGHISSPKEVEMQLKNFKKLQKKKKGTRKTQMEQQHNLTNHPYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNX6</accession>
    <name>A0A1E5RNX6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Centromere DNA-binding protein complex CBF3 subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g911</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNX6"/>
    <dbReference type="FunCoup" id="A0A1E5RNX6">
        <property type="interactions" value="103"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNX6"/>
    <dbReference type="InParanoid" id="A0A1E5RNX6"/>
    <dbReference type="OrthoDB" id="1747771at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031760">
        <property type="entry name" value="Cep3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16846">
        <property type="entry name" value="Cep3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="8"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ88580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="583" mass="68377" checksum="5BFF07398DD68AE0" modified="2017-01-18" version="1">MSNQINRSCVRCVRKKIKCDRIIPCSNCVKRGLEDECVLPSETALSAGEMAIKESFELQILNVMHQWDYWVVQQGFCNGLLLPCKVYTLDYSKENWHPGVIKLIDLEISARLLTHSVESIGCLYLNFFPDIQELFSKFDTFLSVGDSKTSNIGDLMNEALLWSLLTLAMYYLDNSVLKELGLPNELQELSTNKLYCEFLAYTIELIQKIQHYPDVRIVQMFLILSNTFIRYEFPLFYNHFLTNVLQISQILNLNTCFKTGSGSSTTSMLIKNVSDRMWYKLLYWDYKTESFNRPISLHSSLFTSLLRHAAFLKSQNPEKYVREDSLELLQWKITSLERDLDQYTHKKPSLKTLEVIRRELEILQVKSSQIKRLQFPESENEKFDLFVINLTIFSTHWKLLQMVIVFYYQDVFSLNELFEYSNLILQTIERETKFIKHPMVLRILALLGSFHGFFYVFNKNQENHLLFKKIRELLKSLNFAKTEKYQALLALLTRFSELKNMFQTRALTDTELVQSVPIVILENDIKILTNQAENRIPNVLEDVGPVLDSAIEEHTQYDTETRNYIKSEQNKLFQILASFKDFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNY9</accession>
    <name>A0A1E5RNY9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Mitochondrial FAD carrier protein FLX1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g793</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNY9"/>
    <dbReference type="FunCoup" id="A0A1E5RNY9">
        <property type="interactions" value="92"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNY9"/>
    <dbReference type="InParanoid" id="A0A1E5RNY9"/>
    <dbReference type="OrthoDB" id="428293at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015215">
        <property type="term" value="F:nucleotide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044712">
        <property type="entry name" value="SLC25A32-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45683">
        <property type="entry name" value="MITOCHONDRIAL NICOTINAMIDE ADENINE DINUCLEOTIDE TRANSPORTER 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="14"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="132"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="235"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ88605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="331" mass="37469" checksum="933B42943D9ED8D2" modified="2017-01-18" version="1">MTLHKPTNGNIVLTPVQKEIVCGLSGGTMTILITHPLDLIKVRLQLSRSSNTSSYKGIIKNTILHTQQNGSQLKNVVKEAYRGLGINLIGNAVSWGMYFGLYRFYKDIIHDTFVPKNTFDLAQTNFTKDQKMNSFMYLGAAWGAGITTSVLTNPIWVIKTRIMGSTKYDPANYQNYHKIKSTFKDMIHKEGWKSLCKGLVPSILGVSQGAIYFSCYDTLKIRWFHSEGDQVEKKLNALDYITITTLSKMVSVTSVYPLQLIKSNLQYNKDTSLSLAQLLRNIYENQPSIGSSKMSKKSWRHFYKGLNANLIRAVPSTCITFYVYETLKHCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP64</accession>
    <name>A0A1E5RP64_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative transporter ESBP6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g730</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP64"/>
    <dbReference type="FunCoup" id="A0A1E5RP64">
        <property type="interactions" value="443"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RP64"/>
    <dbReference type="OrthoDB" id="2213137at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17352">
        <property type="entry name" value="MFS_MCT_SLC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF315">
        <property type="entry name" value="TRANSPORTER MCH2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="261"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="357"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="390"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="422"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="479"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="542"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="567"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="602"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="633"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="63"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="114"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="175"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="187"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ88659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="754" mass="84233" checksum="A7F98DD2E3301315" modified="2017-01-18" version="1">MTGSLKREISQDYNDSHDNESCLSSEEIQSLDSNVIMELQNHSAHIPLINQIDKNQRFVSKYDHTNRVSRVSSKNGLSKTSTNKSMHSVTSNISKTLSYIKSCVQDDNLQTEQEKCDATNFDNGYRGNREDEDEDDDDDDDDDDDDDDDDTHREEELSKILTKNFELEDALRLTASHQSRYRSPSTIEKEHKQQDLEKGQYANIQTFFGNTEIQEENKTKLFDQESSDDDKSQSGKETLEKVFTNYNTNTLDLPPDGGRNAYVSLACVLVLMFCTWGANASNGIILAFFLNANVFSDGTPFKYAIITGLSLFLGQALSPFVTLAVKIFGFKPPMCFGVVCTFLGFILAAFSTKLWQLFCTLGVLCGFGISFTFVPATAVLPGWFLKKRSMALGVSLVGTGLGGFIFSLSCNALIERDQNQKWALIQMAIITTGLSVFAVFFIKQRNPPPRTHVSGFYGFINLLKTIFDINIMKKYITNLISIWFTLAVLGYMLMVVSLSSYARARGLSANQASYLTVYLNVAQAVGRPIMGLSGDRFGRSNVTSTLTTVIIILIFGFWIPAHTNGQLVAFSVLIGLCIGVANVMNTVLLADSVAPKDFLTSWSYVNIVGSPLFLFSESIVQSLTDSKNIANPYFHAQIFAGCCFVAAFCLSLLIREFKVRHLIKERLQATLTNLQDYDTESITSANGYEEVEKKKKNICNGRDIGAQDIQLTAASVSNNIPSEALLLERKQKYEYLLKNNYKSFFFRAFYPIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RPD2</accession>
    <name>A0A1E5RPD2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein MSS2, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g902</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RPD2"/>
    <dbReference type="FunCoup" id="A0A1E5RPD2">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RPD2"/>
    <dbReference type="InParanoid" id="A0A1E5RPD2"/>
    <dbReference type="OrthoDB" id="1658288at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="196"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="369" mass="43070" checksum="5DB89F86A97D6920" modified="2017-01-18" version="1">MMQTYCAVRKYTTEAPKMLTSRPRLKDILPKKSFFNRCLYDLKADNSFTSMYPTVKQIYKNMGPDGSLTKVKYPKWFTSTDLMNFKRCMENYRVQHKRINKNLFELENTLLKIAVDMNDSNAKALSAFYVLTKSTSFQKEIVLQAQKQLQMLYKNENNVLSMKLLGDLAFNGNNFQAAIKFYRKFITFENNTPLAAEVYKSLGEVLFKLARFEEAEECFLQCIRNCKTQESVKAYYYLGQIYTVSDPLKSKQCFEICASEGFVESLSLLGFLEMNYFGDMVKSCEWFKLGKELGDLKCYIGYFDLQWALKDYRECMFTYKSLQDMAKNSENATKALQTFEESRNSKIESVLNINKEYTPATTEDSRWTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV24</accession>
    <name>A0A1E5RV24_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nuclear migration protein NUM1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g321</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RV24">
        <property type="interactions" value="205"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV24"/>
    <dbReference type="InParanoid" id="A0A1E5RV24"/>
    <dbReference type="OrthoDB" id="2149224at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032065">
        <property type="term" value="P:maintenance of protein location in cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13365">
        <property type="entry name" value="PH_PLC_plant-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053005">
        <property type="entry name" value="Nuclear_Pos-Cytoskel_Interact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024774">
        <property type="entry name" value="PH_dom-Mcp5-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28190">
        <property type="entry name" value="NUCLEAR MIGRATION PROTEIN NUM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28190:SF1">
        <property type="entry name" value="NUCLEAR MIGRATION PROTEIN NUM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12814">
        <property type="entry name" value="Mcp5_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="2064"/>
            <end position="2182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="36"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="442"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="520"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="997"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1154"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1387"/>
            <end position="1439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1460"/>
            <end position="1558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1584"/>
            <end position="1618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1670"/>
            <end position="1691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1705"/>
            <end position="1728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1835"/>
            <end position="1918"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2014"/>
            <end position="2034"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2198"/>
            <end position="2296"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="88"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1192"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="36"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="442"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="454"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="520"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="997"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1044"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1161"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1387"/>
            <end position="1398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1401"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1468"/>
            <end position="1478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1485"/>
            <end position="1494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1520"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1543"/>
            <end position="1556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1600"/>
            <end position="1618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1705"/>
            <end position="1719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1846"/>
            <end position="1858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1876"/>
            <end position="1914"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2025"/>
            <end position="2034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2198"/>
            <end position="2217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2230"/>
            <end position="2245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2255"/>
            <end position="2272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2278"/>
            <end position="2290"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="4">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ90781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="2296" mass="256941" checksum="478107F59B030C1F" modified="2017-01-18" version="1">MDSRRNSRQDLRAQLENLEKNMDSKLIKRSTSLYGLNKDNQSLNTSQSSTPAEKTALSNRNKSNPMRNSIHHVNDREPENFKFMKDLSDNLLLQCRKLQRELDQSKKQVTSLELQNQHLTLTKDNLDSHNLKLTNELSSLQDEVREVNKTTNVNVNDQNNVVLSQYNYKNLLNNSVDRISKEDLLKFISSRFNLRAIPYGEYENLIKPMNEQLLQAEAARLGFVAMPRQNFDLIKAKSESPDEKTVTSAASKMGLTLVKSKELHNLKNPSKHELEKKIKKLGFTMVPENLSTSNTSESNLSQVFSNGTPNASSISLQSATSSPMKSKFAPSSKVSASREFFEQVIREENNKKDVILESGRSLGFVRLSTDQYKKLLEGQKEHVLTKSDIYAGAKDYQLTVLPNEEYKALLRKKNYTDSLSFDDINEFAKRLQLKLIPLASNDSSVENSRRSSSQLGHITNNHAHSMLLPGSSTLRSRNYSTDSLLSSTDLHDPRASADSTLLLNNFKQTQDKNFNKTFTKDDHHAEKVDNGLXDDSHEMTSKSAQVVDDDLLSKKPSSTSEMTLEKLTETCKLKGYVMLKQEEYATLKKPPTIEYLKEHVAKNNLKLLTPQEVSKLKSKEHLQEMLEDKYKVVSKEELAKLQQPTPVSLASVKXFVNTNDMKILPLKEFEILQNDAAKPEVEGKRLQDFPVQDLKQYLEATDDCAIMKKADYDDLMNPKPRSLDEVVKDLQSQFPSQVVIERSKLEHLSSKELSLDELKQNLSSMGYTAVSDEELSSLKTEVADLLKPPALEDLQAIASTKYGYALISEEEMAELQKGKTPTEEQLQAYAPQMGLALLKQDEYVLLKSAAETSKKYEDMEPHTLQTIAESKGFQIIPPNELKELQATAEKVKTLSAAKNLSKTELMKQASQNGFSMIPQAELKEMETKINNSKITLENLQNQCEKLGCVALPISEYEELRSQVTTTGELDLSKEAEKQGMVLLSNKDYAAMKENPHFLTEHKKDPQKMISTDGLPLPNDKNGENKENQSISNESTGKHLVGEQLSKTESVNDNQDNKFSTELLQKEASKLGMLVVPKHNYELPKESPSEGNSQHLNIHEEADKLGLILLSQENYTSIMRELDELNKQSERSVDIEKEAKKQGFAVLKQNELESLQQSASSESKEKEESAPTVEKVEKDAKKLGLVTMNKLEFESLKEAIVEAQQIARNELENSKPNIEEEAKKQGMIVLAKSDFEVLKQTVEEAQNAVKLQLPNDATELAIKDAAQKQGLIVMTKEEFDSLKELVMEAEIIQEKADENQKDGINQENEVDNPAFATIEQEALKLGLIVISQKELDDLRAYAENSSVGKHISSTPYNIYEEAKKRNLLIMKQSEFDFMQKQIQEGGNHNELKQSTKAEDSNSEISQSSNNRSFASAKQATDAGAQNSEPFYPKNIVDPAATKPSTEMESTLLLNRQEASIPDTPCEASVKSQSTQSSAFSPEASAGKQTLQSSGVPTEVAAGKQTLQSSDVPTEVAAGKQKLQSSDVSPDASANTHNISRDNQRVASPTNGNPSPLNDSLAVSRLKEHALHTDSEFLQPEISHTEDDSFHTATELSVKPEQGIRKTEANEPPAAEHKDVAQNEWDLSVISETAKKHDLVLLSKVKYRKLMASSRANHQDFSFDDDFESTKRTIESEEGSENGNAEGKTNIDKNISSINQNELVQSERNYQNNGKPTTQTDDSYEPASIDQPKQAFLNKNIITERAKEFGLIAIEESQFNEITNQLASQELSPEEIVMKAREYGYVSIEHDQFAEIKEELENPHLSKEQLARIALEQHNLVLIDKNEYRELKKSDLNDYKNHQPQGRSFSGSVPSSPSVSHNPGNRANSLIKVRQPIRQVSLSNPTAHQRQNSVSSLHPPQLGSSTVQPSPASESNGAVFVPALPAPGSFRSKSNRANNSATYNFKEEAKKNGFLCLPESCYIAPSSAATTTITPSQDFLNEGQLIDSDRVVVLPKEHYTALLESYTNAQTNMINDSASHDKRSGGESVSRTHQVHDVSNVTRDDISLKSNASLTDYNVIAALTQTVIGEYLYKYYPKFGILTKVRHERYFWIHPYTMTLYWSHSNPAMSSNVSSSGYDDKTKGCAIVDVLTVEDSNPYPPGLYHKSIIIRTDHHKTKEIKITCPTRSRHNIWYNSLKYLIENNNVSSAHASSEFKQVSTQVVKNTSAHPTRQTQSAHSISGAREQNDRHSSSYNRPPSDSNEQNSYFFVKSLADGNNSNNNNNNNNTRSTSSNKRAVSANKQQSRSASFLSKTFGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV89</accession>
    <name>A0A1E5RV89_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Protein dopey</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g337</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DOP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RV89">
        <property type="interactions" value="129"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV89"/>
    <dbReference type="InParanoid" id="A0A1E5RV89"/>
    <dbReference type="OrthoDB" id="297643at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040314">
        <property type="entry name" value="DOP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056457">
        <property type="entry name" value="DOP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007249">
        <property type="entry name" value="DOP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056458">
        <property type="entry name" value="TPR_DOP1_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14042">
        <property type="entry name" value="DOPEY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14042:SF24">
        <property type="entry name" value="PROTEIN DOPEY-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24598">
        <property type="entry name" value="DOP1_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04118">
        <property type="entry name" value="Dopey_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24597">
        <property type="entry name" value="TPR_DOP1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DOP1 N-terminal" evidence="8">
        <location>
            <begin position="23"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="DOP1-like middle TPR" evidence="9">
        <location>
            <begin position="370"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="DOP1-like C-terminal" evidence="10">
        <location>
            <begin position="1224"/>
            <end position="1369"/>
        </location>
    </feature>
    <feature type="domain" description="DOP1-like C-terminal" evidence="10">
        <location>
            <begin position="1390"/>
            <end position="1758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="235"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1375"/>
            <end position="1398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="240"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="11">
        <location>
            <position position="1521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ90867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1786" mass="202493" checksum="A31959F8A41F41AD" modified="2017-01-18" version="1">MNTPKEAPRLVPFVEKKTAKVDYKEKQFSSNVEKALQQFDSVDDWADYISRLGKLLKALQSWSPKFQNVSYYVPHPYQVARRLASSLAPNLPAGVHSKTLEVYSIIFDKVGIETLSSQCNIWIPGILPLMTYATLSVKPQLIEVYEEYILKMNSATLKVLVKPILASLLPGIDEEGSEFQSQILQLIMTLQEALNNDSLFWQSFYLIMIKDKDHRLGGLVWMTKQMPSLNAIPHLATSSDENKXQKEKNAXSTGEETSTKEQRKTALELLLPKAKPLVSLEVGLLIRCLVACLANDNEIIIQRGILDLILQRIHLDSPVLETLIFPSEKEMLVMACCEQLLKKDMSLNRRIWSWFLKPSSKNEEEPDQQYFVEHCLETLFHGLHNYIKNGRLVDALKISQILMHKWEIGSLVTGXMFLPLTKASIGENEKTILAASEYFDSIDTKIIWNSILSTLLESDYSVLNFVLDNYHLENEEEMVVKHLPMILLELLVLPQERNYPLLMKLLRLIPERAYLIEPQAASFKSLPSDEEIAAKIXEYYSNDNAMAPFSLITLTHIILSKLYALLLSSLETGKHVPESIELFVMLFSLLPEAELITDFDYKKVSDKLIESKYKIGDDNYVNVIPLSNLFVAILLTKIDTPSMIKNLRFLFQRLWVLLTDPSKQLEALQALENLQRVIPQKYMEASIVSVFMEEKEITKKLLVMNGLWTQSNKTSIVTNNSTSNYTLAAKPLELILDELADESKSEYLYAKEWLLNIFDDTKSANRLFEIYGWNLLNFDFINQKEEXEEFDNIDIFTYYVNTLINVLNCSDGQGLALLNSELTSSSCANLFLSTSKDKKVNISTYKNLFVAILLKFLCIKSNKNSRSVRVVLKFLHMVLNGSEYNFKNTVSLFLDLSSSYIQKSSSHETDLIIVALLDIVSNVLSLSQKRNIKLEIFDNSQSHLKYVDFLVSSVLLIKKPSIMKAYVKLLTESILYFREAVFNIILPLISAMVASVEALYSEEYGTYENYDNYLSVSSLLDGIEELLQVSHSYMNVSLSHLNGGNKSDFLSNVVSNVFSGVSSSASSGPGTNGSPESNRILVNRQVVVQSFAATIKCCFKIWKSCNSYEQDLDRSTAYSSSLYQQSCKYKYKARKLLEKLYLLEPVESSQCLLDMKNEPASIHSIYKLVYALDGNRPTLTLPHFFRMCVYKHNPGSTLLNQDFKVKMSTSNSFYNVDSALLMDFVLIYVANLEFSAVEDINTAFLQFMKEVVGNASAYENISAQFLELAATVCYKMGHCQFGEDKRLRKEYSDLFSKYLGLLDYSLLQLKNRKIYADSLQTCVQYAEYIVADAKFSEKYNFLLSSLIGTFVAPFMKKINANSSISTEFLPVDSVTSNDESAKDRSTTKTASKRLSRQTKENSIDIPNYVTEFMSFIAPLGSKVKTWKAMMSEFFSMAPNDDYSKSSKIFGNAFSNCLFIVDQSSAWKIIIYEWSQYSDNKGRLINDLVINCSAATSNPFGSWSQTEVGTKCLDYFKICYLLLISPKDEHILQFKPLMNEVERVCLNENGDIENEIMCMGFKLLRCMFLKFNKMQFLNNWGSITFILQKNLQVLLESEGKIDMQLVFQIFKTLDMLVTLNFEEFDSTQAWLFIVDSLKGISSDEYVSLVDLLAQSGKIFDLSGQGSGSANKETLLPSSSTTAAASLASTTTATTITTTTPSSSSSVSTTAISASGPGKLLSMIEKHKMPLLYGKHDALTQMDLKNFVEIFSYANYESLFNDSGAHYSNEEILEDTLQDLFYSPQFFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVA8</accession>
    <name>A0A1E5RVA8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">GTPase-activating protein BEM2/IPL2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g442</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVA8">
        <property type="interactions" value="628"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVA8"/>
    <dbReference type="InParanoid" id="A0A1E5RVA8"/>
    <dbReference type="OrthoDB" id="79452at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005933">
        <property type="term" value="C:cellular bud"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050729">
        <property type="entry name" value="Rho-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF96">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN BEM2_IPL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GEF" evidence="4">
        <location>
            <begin position="709"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="5">
        <location>
            <begin position="1316"/>
            <end position="1480"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="6">
        <location>
            <begin position="2211"/>
            <end position="2409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="118"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="280"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="377"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="443"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1985"/>
            <end position="2082"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="45"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="57"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="84"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="118"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="130"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="289"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="377"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="391"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="446"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="469"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1996"/>
            <end position="2046"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="2047"/>
            <end position="2068"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="7">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ90887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="2413" mass="274416" checksum="6800B7EDAE861F32" modified="2017-01-18" version="1">MKSFLWGNKHHRKDDEIEQSPRISISQPSNYQHLTPESLNGAXETNNVNNGNTSTTQKLSXPSIHENNNSNDFEXSKQNVNRISSTGSSSTMRRSGLTRSNRSSTISTYLDDSYDYTNESLSRQTPNLGQKQQKQKQQQQQQQQQQQQQQEQQQQQEELRQXNFNTMPEPQIESQPQHTLTQEQEQQRQEQRQITPRKPKTSISALNATMEDMKVTXRTSDLQNSKNRQSSTPKRNVSRALRNYSSTDNIRKSMNLSNLRNSFSSSTVSLGTHSNIETVEENDNDDTYNNVNKQNTNSNSLRNPFVDNGQGAKRKIRSVSNVATPVVQTPKTSSQMTLSKDNYDSYVFKTGWLNKTQSGLPNYRESKLFYSTNIDHNNESSSSTSVKGHGASDAASDSSSYSDNYLPDYRLYKVSLQGSVLNFYKSWNNNIKYFDPNIKYEAPNESKVSSSSNTQFXSNKTPSKKEPESSTSFASPDQTPKHSPITENNCAKNDQETLAPQELEQQMKFETFWPSLVYQEADGVHPDLRFDSASPSQLIFHDSLEGFMHYLLFADKLTSADVENYVLMLPIITSLEESSKNFTNPSERSNGMLDALKYMYAFLKHFNSXVNNNIEFGKEQLMISRAKFVLETIKSSLLGYLIDSNCFKVMIIIIDLLRNFHSDYDFKELKQEFANDKTNLDSLLLNLRQXVLPDQKSDLLEPNSFIALDLTDIANAVHFLNSSFNSVWSPEKDYTLLYEIPLSSNSLNPLMFKNQENVHFLSKLMVQHLNTTLLTTVSDAEVNILAKKRARILSKWVQLGCTFEKLGDMVSWLAIATIICSVPILRLSITWKYVPDSILKTIFKDWTPTISQLERKNFCFSSSNIFILAPPEMNXNNIESIVPYFGDLLISKEELGIFFAEFESRNSAKTDSASTLIESSSNSETRGAKQNYKHLLRKINRTKSAFAKWKLKSNSLKXPQSVATAQPLLDFQSKFETFFETYPSSGKLFQQLSTFWASSSKPDQPSVSLTNIMDLSLKLEPPLVNDKLYSQISSRRSPLLSGSYLPIIFNDLLPNYSLFSKAALIGASGAKNGKSNSEMSNSTTSIQGIPKSVSTRSVILTGNSEIDDSVADLTQQTSSLVSNKQRFLKSIRDAFNIEMNDFHIKDEIILKNVYEDSAKSNSRPASMVIETPKRTSSVSMSGTLTTSPFRHSTSSSHFSALDNMNMFLKNVGNETDKISSENFVHVVLKSCTLERMFDILVLTTAVFSKLVNSNDVKNYFQALQTKIQENRFALQKMPSVKSNSSGNKILLHRSSVFADGNDNAQTSSTKRCSSNAPFQAPPPTFGPLDMPLINFVLDNDLFTETFFNTYKSFTTSSNVLTSLAKRFVSAQSCAXSIDNLEKNLTTDTTDAFLLDKETRFPKWDIKKQEFPEFDADMWARVANIQIRVMEAMYFFVKEHFSDFTVSLENHATIVDFFKIVDDELKHDWKYRVKKFSQNEKAAEYLDEKASLEELMPPTDLAGPAEVTKQPIVASSQELPGLVEKLANLFNKTKASYQKHYFRPLNYNKQQKSILNNLSNFDSVSPFEYHSKFSTNGDPMYSGFVNMKYDQYESIIDWVYKIDDVVVEKLKMVKVNEWLEAGQFLDMFSYSSLTSLFNNDLHSYNWELVSKTPMNQVAYDNLEISNIFTWLTSLTYPEHNKGNDDASSENFKAHPKHVMVQLPYSIQMIFKFHLSLTSFFMLHISNPYLTQEERSNVFCKILQLLLFTRWKNSQVDLFDEESSDDQPDISPHVPSFLEACILEAILAPESRFYEMTWELAYQKLSGTTDPLISMSTMITNVDRERIVRFVEHDGINKRKNLTPCPGWFINRLLEISQFVPNMSIENSKLINFDKRRFLNNMVSNYCDMIPELDSHSARQENDKNFAEMLFHDVSKSMILSYRAQCKKKAGEESSNANYQNHGLFNDVISLELGGVLRNQHKYQELKTQEEQTRSAVYVKDIKSVKNKNDKTNDARXSISSPSMANFQNKTFGPTSTYQPNKSRMSNSQSIISLNSTMGSQTPSVSAGSSTKVSTKRSSVVTSAKSSRSSSHGEPHQPRSSRIGGFFKRPFSLSGFSSSNSNNSLNSYLANNKGAIAPQALPVVTLQEIKDTKPQISVRTFEVKSCMPLHSYKHNPHCSDAFKICMNNGMEYCVQATSLEDMHEWITMINKSKRYSFYSIRYRGKTHNKVFGVPLEDLCEREGVFVPAVVTRLLEEIELRGLDEVGLYRVPGSVGSINALKQAFDTYGAENNNNFTLEDDRWFEINAIAGCFKLFLRDLPESLLTENRLDDFIXVMAQFKIDNDKELMETNLRILLKQIPTCNYHVMKRVFQHLNRVHSHFENNKMDATNLAIVFSMSFIGKDDLSMTVGSKLGALQTILQHFIRSPQTFFTDSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVI6</accession>
    <name>A0A1E5RVI6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Exosome complex component MTR3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g520</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVI6">
        <property type="interactions" value="173"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVI6"/>
    <dbReference type="InParanoid" id="A0A1E5RVI6"/>
    <dbReference type="OrthoDB" id="2504340at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF2">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT MTR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="9">
        <location>
            <begin position="38"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ90859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="250" mass="27660" checksum="38EDEF4ADE61C3B1" modified="2017-01-18" version="1">MNVQDRRRLLGPANAKPIVFTKPSKHATTKKTYCSISNMYIKTGLIKNCNGSSYLELVLEDGSKFLLITSVYGPKPIRGSFVSKAAVSVHINSDTNEIATXGNILSNDFDVMFQNDEETLKDLSGYLKNCFDSTIQLEKYPKSGIDIFVNVVSSSNLQVSTPFFLKQSINGILXALIDANIEVSDLIGSGVSNNNCITTFIKNGQEIVGFWKMDDDEAEDYAASVAEVVEVCKQDYFANKKVLXQFLEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVK2</accession>
    <name>A0A1E5RVK2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g528</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVK2"/>
    <dbReference type="InParanoid" id="A0A1E5RVK2"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184896" evidence="2">
        <location>
            <begin position="23"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ90865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="118" mass="12278" checksum="1F1458D1BF9EF536" modified="2017-01-18" version="1">MFSKSVVLFFSVAAMLVNFAGAQVESSSSIETYYETVTSCDSIPADSTDSAPFDSSTALSIVDSTLLEIVSATYYVTHYTSQCGADVNSGSTTTTSVSSSTSTSSISLSTHTTTVFAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVU0</accession>
    <name>A0A1E5RVU0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SPX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g427</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVU0">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVU0"/>
    <dbReference type="InParanoid" id="A0A1E5RVU0"/>
    <dbReference type="OrthoDB" id="5588846at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033254">
        <property type="term" value="C:vacuolar transporter chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016237">
        <property type="term" value="P:microautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006799">
        <property type="term" value="P:polyphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14474">
        <property type="entry name" value="SPX_YDR089W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051572">
        <property type="entry name" value="VTC_Complex_Subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140:SF1">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE COMPLEX SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="981"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1020"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1056"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="9">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="214"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="565"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="673"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="228"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="574"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="678"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ91032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1078" mass="123893" checksum="B8129ADE0B94A6D7" modified="2017-01-18" version="1">MKFGSQIFDKSVPEWRYNNLDYNKLKKLIKLCTTTQYSNPDHLVIMKQHFEDQFLNVNIFINLKLNEITSRILVLEEQLLALKQKKDSLSSASTIKSNQQNESHYQPLNTHXRTNFSKTKRVFLKKIDKCNQLLTNLSRFLIVQKIAIRKLFKKFIKHYPDRQRGTQFIDELQSSDIFTKGYNGVSIINVDLDPYLLEVSLIMDYLYSDNTEXELQEEEEQRQRKNNVDSSSRTNINNKKKTANSSKVFHDECEFDGLMLKNNFKSKKFMVSGESSMQLKFMLMKLGFLLIAPGFFEKALTTTISGTSSAANAADNTYSENNNLHNLTVPEINALNRSRSFQNLRKQSETSLLSNQGLESDFEKISLKLLNRPLSNNGHQKLDIETSKDINTHPNLVISYITQQQQSAPNDMLHNEKCCVQCXXACSANAADNTYSENNNLHNLTVPEINALNRSRSFQNLRKQSETSLLSNQGLESDFEKISLKLLNRPLSNNGHQKLDIETSKDINTHPNLVISYITQQQQSAPNDMLHNEKCCVQCHIGGIRQYIVTDQVPYTAIVDKDSEELLNPSDPMHSSTHTNLSSTTTNTTNTTNTTNTSSSLSSHPLNKMIIEWIQSKNLQVVHPTLEFKRTRFALFDKTTKNIKYLCTLDENFNNNEFIENNAFLTLINLEPESQKTPPSSNXSHASSPASNKKKQIDTRLNNEIEFFIDFYKNLKANNIMAFPTNNTLWLSMYHSQFYDMQDFYKFTMQDFKDSHFYIDDLITNDIFYFVGGKVIDFEVKQLTERQKTLNDNHSYILKKPHRNAVALLNTDPNSQNLLYQHQVGESDENDNQQHPQPQQLRYWNEFDDGDEYDNGHSNDYNYYDENEDYDDLENGALYPDERFIKISKNFINTAYKVFFPRVPLPSLSSLEHLRLGRIRSNGNSENSSLLDSPTLFDHPYESYGEESHTRNGSMVRKANFLSLEDSMIIYEYKHDQVFSVLYILSLVLSCLTCGVSIGIVLSVFDLIDEHSSELEISSLFIVILICSLLGALVFGIISLLLLFSRFTKAPLWHYMSGFSLFIAVTIIACYGILGLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVV9</accession>
    <name>A0A1E5RVV9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Succinate-semialdehyde dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="3">1.2.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">NAD(+)-dependent succinic semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g480</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; 4-aminobutanoate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.24" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVV9"/>
    <dbReference type="InParanoid" id="A0A1E5RVV9"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004777">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07103">
        <property type="entry name" value="ALDH_F5_SSADH_GabD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000007">
        <property type="entry name" value="NAD/NADP-dependent betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000004">
        <property type="entry name" value="Succinate-semialdehyde dehydrogenase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050740">
        <property type="entry name" value="Aldehyde_DH_Superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353:SF5">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="9">
        <location>
            <begin position="61"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="311"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="10">
        <location>
            <position position="275"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="10">
        <location>
            <position position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ90969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="541" mass="59589" checksum="F17C3FFBDF610EEA" modified="2017-01-18" version="1">MLKYRLGFKVNPLSSALKRHQSTTNTSVKKVLDILENKDILPSTQAFINNEYVGSSSTFNDPEDNMFDVLNPSTLKSITKVHNMGVSDYNKAIDIAHKAFEESRYHITPRDRSRWLYSMYELLKENRLDLAKIITLENGKTLKDSLSEIDYSASYFQWFAEEAPRIYGSIISSSSPLSSLQXAGSTFKEIQARRQPLGVVGILTPWNFPSAMIARKLSVALATGNSCIIKPAKETPLSGLAYGWLAKQANVPAGLVNILPTTYADSIGKLIVGNDLIKKVSFTGSTKIGKLLLKQGMSLDNNDTLKKYSMELGGNAPFIIDQTADLDKALSGLVNAKFRQSGQTCICANRVYVHEDVHDKFAEMLKTYIEENFKLGDGFDSMVTHGPLIHSKALDKVAALVQDAKVKGAKIFLGGTRASSIGPLFYHPTILTDVNDSMKIYHEEIFGPVCSLIKYSDSIDTVIQECNKSDVGLASYFYSQDVQSIHKVTQQLECGMIGVNSGSISDTALPFGGTKNSGFGREGSLYGVEDYTEVKSVVYEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZK5</accession>
    <name>A0A1E5RZK5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZK5">
        <property type="interactions" value="68"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZK5"/>
    <dbReference type="InParanoid" id="A0A1E5RZK5"/>
    <dbReference type="OrthoDB" id="10267139at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043743">
        <property type="term" value="F:LPPG:FO 2-phospho-L-lactate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10680">
        <property type="entry name" value="CofD-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002882">
        <property type="entry name" value="CofD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038136">
        <property type="entry name" value="CofD-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31240">
        <property type="entry name" value="MATERNAL EFFECT EMBRYO ARREST 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31240:SF0">
        <property type="entry name" value="MATERNAL EFFECT EMBRYO ARREST 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01933">
        <property type="entry name" value="CofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142338">
        <property type="entry name" value="CofD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="263"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="535" mass="59571" checksum="7F62985710754EBA" modified="2017-01-18" version="1">MYTANNNQNTGKKLESLSVTTDNKAIKDNGNKKSIVVLSGGTATNSLIDSCFSPALYNKVTFILPISDNGGSSSEIIRLFPGAAPGDFRSRIVKLIEPKSKDDIELAKIMNYRLNQENIDLAKKEWNDIIEGDHQVWXECSSILKHMIRSFLINVHIEILKKQQLNPSAKHKFDFRNANIGNFFLTGCRLLIGASFDSGLELMMRICKIDSNKTKVYSCINTNYTHHIAAILGNGQIIIGQSQISHPSSVSVLSNSEDGLNAVSSSQSHXQNSVQQKNHEEDGTITDDEFDVDIKSPDEDDNQKRIHPDLKISQLNFTKSMTHSKLPSPIEKIVYINSYGEIIQPLGNPRCLHSIKHANTLVYSIGSLMTSLLPILILPNVAEAIVCNKKMKKILLVNSEVDRETEQFVDTLANAQKDTADSSAACSTNTNAFVHMIVKSIYNNLPPKYKPFNLSDNNNNNNNNNTMNTSPQDDEIPIXFYKEVLTDVIYLKKGDIPIDGNDLKEKGLNVHCIDSDSQKFEGKDLQEIFETIGAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZL4</accession>
    <name>A0A1E5RZL4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase</fullName>
            <ecNumber evidence="13">2.7.8.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g126</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Functions in the biosynthesis of the anionic phospholipids phosphatidylglycerol and cardiolipin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>a CDP-1,2-diacyl-sn-glycerol + sn-glycerol 3-phosphate = a 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycero-3'-phosphate) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12593"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60110"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Phospholipid metabolism; phosphatidylglycerol biosynthesis; phosphatidylglycerol from CDP-diacylglycerol: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the CDP-alcohol phosphatidyltransferase class-II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.5" evidence="13"/>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZL4">
        <property type="interactions" value="426"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZL4"/>
    <dbReference type="InParanoid" id="A0A1E5RZL4"/>
    <dbReference type="OrthoDB" id="10250191at2759"/>
    <dbReference type="UniPathway" id="UPA00084">
        <property type="reaction ID" value="UER00503"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008444">
        <property type="term" value="F:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09135">
        <property type="entry name" value="PLDc_PGS1_euk_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09137">
        <property type="entry name" value="PLDc_PGS1_euk_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000044">
        <property type="entry name" value="CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016270">
        <property type="entry name" value="PGS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12586:SF1">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12586">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--SERINE O-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000850">
        <property type="entry name" value="Phospholipase_D_PSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 13" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 13" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11 13" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="14">
        <location>
            <begin position="147"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ92276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="503" mass="57867" checksum="8B054552B1A54E5B" modified="2017-01-18" version="1">MSTSSSSISLSLFESCKTLLSQSLKTKFFFKPQQIEIIEKPKDFYDSLKQGISNAKERIFFASLYLGKNEQELIQCVSHALQKNKNLKVYFMVDGLRGTRETPLPCSASLLATLVRDHDDRVDIRCYKTPRIFGLKESLIPKRFNEGLGLQHMKIYGFDDQVILSGANLSXDYFTNRQDRYYCFRSKSFADYYFKLHQLISKLSFRALYSENNAKYQLVWPDDNLAVNPQRNRQRFLSQASTVLKTFLEKDHTKDSLDEDTSSYPTVVYPVSQFTPLFNTFETLPHRYQDSKHDYSTEKPTILRLLDHIGGLQKTCINWTFTAGYFNMLPEIKQRLLMNSVQGKHTCGKVITASAHANGFFESKGISKYLPEAYMHLAYKFLREVARNGSQIDLYEWKKGIVTRPDGWSYHAKGIWIGEGSNQPPTVTIVGSSNYTKRAYKLDLESNCIINTRDPLLQEKMQRELENIMANSEKLSLQTFKDDPSKQVSNGVKLATRLLGDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZR2</accession>
    <name>A0A1E5RZR2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">DNA replication complex GINS protein PSF2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g20</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Component of the GINS complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the GINS2/PSF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZR2">
        <property type="interactions" value="697"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZR2"/>
    <dbReference type="InParanoid" id="A0A1E5RZR2"/>
    <dbReference type="OrthoDB" id="1938138at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11712">
        <property type="entry name" value="GINS_A_psf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21694">
        <property type="entry name" value="GINS_B_Psf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1020:FF:000001">
        <property type="entry name" value="DNA replication complex GINS protein PSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007257">
        <property type="entry name" value="GINS_Psf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056784">
        <property type="entry name" value="PSF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12772">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12772:SF0">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25005">
        <property type="entry name" value="PSF2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028998">
        <property type="entry name" value="GINS_Psf2_subgr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160059">
        <property type="entry name" value="PriA/YqbF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication complex GINS protein PSF2 N-terminal" evidence="8">
        <location>
            <begin position="63"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="GINS subunit" evidence="7">
        <location>
            <begin position="96"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR028998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ92326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="236" mass="27043" checksum="40DE97B83004F71F" modified="2017-01-18" version="1">MSFPESLQTNFLPEEIQFLVENELVKILPRITTRRKKQXNGRSQQENGSLSLNWELISADGHNVNNMIAMRPTELPLWMAXFLKEQKKCNMIPPGWLTASKLEKFLEFEIANPMKFSALPWHWLVVSKIYFSKCPDDLTDPVHILRSKCQDLREIRAAKVHKGLEAMNESHLQLDNLSLLEINESRPFIIGVMNKMKDVHKSTAAHGNNVDNNXYEDXSNIYSNSNAGNASYLSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZS1</accession>
    <name>A0A1E5RZS1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein MAK32</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g71</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZS1">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZS1"/>
    <dbReference type="InParanoid" id="A0A1E5RZS1"/>
    <dbReference type="OrthoDB" id="497927at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47098">
        <property type="entry name" value="PROTEIN MAK32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47098:SF2">
        <property type="entry name" value="PROTEIN MAK32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="1">
        <location>
            <begin position="217"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="376" mass="42021" checksum="41D1DB728380FCD5" modified="2017-01-18" version="1">MTNPTSTETLFTTNGMFIIDEIVNKNTGERLTDIVGGGGTFAALGATIVATAKHNFQQKNXLSEKNKALSVAERIKFIVDCGSDFPYETVQSQLDAWGIGIIYRKDSSRLTTRGYNEYGPGDFRTFKYQTPKKLVLGQDFQQTIYGPIAIVHLVCSAKRAMEIIEALRVESPMAKGEYTYKCKKFVWEPVPDLCTLEHYPDIYNVLNQYTNDDFEIILSPNAEEAFQLFYGIDYKDPSIQKCITKLQSKIHQLSPFPVVIRCGAHGCILFNETDKEGENIKPRVLPAYHKLTQDEVKDPTGGGNTFLGGFAMGLVLSGGDFYQACICGNIVSGIVIEQNGLPALDKTTQPGVYLWNGKSFAQRWDHYMQTFLCMSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZW7</accession>
    <name>A0A1E5RZW7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g186</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZW7"/>
    <dbReference type="InParanoid" id="A0A1E5RZW7"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184959" evidence="2">
        <location>
            <begin position="20"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ92349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="140" mass="15028" checksum="A936877BC3C14650" modified="2017-01-18" version="1">MFFSVYVWVALLSMQWCSAMDYTNTTSSSSTIHDYHHYANMTANTTATMLSSSTMVVYSTKPDTTITSLATKTTTVSRLGLEEDSSFGSSHSLHSTTTQTSGSSSGENSVAATSSLKNKAVPTCGTFPLLWMISSLFACI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S016</accession>
    <name>A0A1E5S016_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ribosomal RNA-processing protein 44</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g87</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the RNR ribonuclease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5S016">
        <property type="interactions" value="1175"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S016"/>
    <dbReference type="InParanoid" id="A0A1E5S016"/>
    <dbReference type="OrthoDB" id="372421at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071034">
        <property type="term" value="P:CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071031">
        <property type="term" value="P:nuclear mRNA surveillance of mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09862">
        <property type="entry name" value="PIN_Rrp44-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000309">
        <property type="entry name" value="Exosome complex exonuclease DIS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000010">
        <property type="entry name" value="Exosome complex exonuclease DIS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.700:FF:000001">
        <property type="entry name" value="Exosome complex exonuclease exoribonuclease (Rrp44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041505">
        <property type="entry name" value="Dis3_CSD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022966">
        <property type="entry name" value="RNase_II/R_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050180">
        <property type="entry name" value="RNR_Ribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033771">
        <property type="entry name" value="Rrp44_CSD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033770">
        <property type="entry name" value="RRP44_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355:SF35">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RRP44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17849">
        <property type="entry name" value="OB_Dis3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13638">
        <property type="entry name" value="PIN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17216">
        <property type="entry name" value="Rrp44_CSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17215">
        <property type="entry name" value="Rrp44_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01175">
        <property type="entry name" value="RIBONUCLEASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11 18" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="10" id="KW-0271">Exosome</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="S1 motif" evidence="17">
        <location>
            <begin position="922"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="333"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00077930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ92399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1012" mass="114516" checksum="2F70093DCF8E4098" modified="2017-01-18" version="1">MTLPANHRKRLSDGLSVTSKVFIRSSRNTANPMKVVREHYLRNDIPCNSKYCSKCSLLMSENNAGTVNSGNNASNPILSDKPDILLPSKTPHYVVLDTNIILQAIDLIESPDCFKDVIIPQTALDEVRNRSYPIYTRIRSIIGQNDELKRFVVFHNDFMEPTYVDRLPNETINDRNDRAIRKLCGWYKSHLTSAYGVEIVLVTNDRLNRESAKKEGITSLSLVDYVERLSNADNIRDIVPQLEPXSAPNASKNAEEFSFPEYYSTARVLGGLQNGSLYQGNVQISEYNFLEGTVSLMNFTKPVLIRGTKNLNRAFNGDQVVVEILPKSEWKTPSNITMDSEHFNVDDNPDGNDEDNDLEEVYGGSLSANTTALISDKERRLLAQEAITTQKGSNKVYPTAKVVSITRRSWRQYVGQIVESSVDKQTPNAVQNCFVLLMDSCLPKIRIKTRRALELMNKRILIGVDSWPATQKYPLGHFIRDLGEVESAQAETEALLLEHDVEYRPFSKKVLECLPTEGHDWKAPADLKDPEANKKDQLLNKRADLRDKLICSIDPPGCVDIDDALHAKRLENGNWEVGVHIADVTHFVKANTALDAEGASRGTSVYLVDKRIDMLPMLLGTDLCSLKPYVDRFSFSVIWELDNDANIVNVDFTKSVIRSREAFSYEQAQIRIDDPSQTDELTLGMRALLEMSKKLKQKRLDAGALNLASPEVKVHMDSETSDPSEVEVKKLLATNSLVEEFMLLANISVARKIYEAFPQTAMLRRHAAPPSTNFEVLNEMLQIRKGMSISLESSKALADSLDRCVDKDEPYFNTLVRIMSTRCMMAAQYFSSGQYSYPDFRHYGLAVDIYTHFTSPIRRYCDVVAHRQLAGAIGYEPLDLSHRDKNKMEMICKNINKRHRNAQFAGRASIEYYVGQVMRDNHSIETGYVIKVFNNGIVVLVPKFGVEGLIRLENLTDDPDNVEFKEDEFKITFTDKNSNSKRDVFVFDKVEVEVKSVLDPITNKRKAQLLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S048</accession>
    <name>A0A1E5S048_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RNA-binding protein VTS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g222</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Monomer. Binds to RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the VTS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5S048">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S048"/>
    <dbReference type="InParanoid" id="A0A1E5S048"/>
    <dbReference type="OrthoDB" id="2155283at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09556">
        <property type="entry name" value="SAM_VTS1_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050897">
        <property type="entry name" value="SMAUG/VTS1_RNA-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037635">
        <property type="entry name" value="VTS1_SAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12515:SF5">
        <property type="entry name" value="PROTEIN SMAUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12515">
        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN CONTAINING PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="SAM" evidence="9">
        <location>
            <begin position="460"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="303"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="403"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="21"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="72"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="332"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="346"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="364"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="379"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="408"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ92375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="531" mass="56941" checksum="84B2D8808A6F11C1" modified="2017-01-18" version="1">MNTPPGQLSPRMHPGAVLMSPNGSYLNGTNNASTANGSNLSNKNNTSVNMQGHYLPRSTLDDLIENTDHPLSPTTSNPSAQHMNGSYLDSFASPSQAPLDSDLISYQSQYHKKTSDPLSSATNLSNNFIDANYGSSKSSTVVNFTQDVNQLIYWMEKLSVQQQNTVMDNLLSSLNEEVLQYTKLKLDSLIHTGYISPSPIGSPAMLAHNLNTSNNNNNNNNMINGFDLNNSGGTSNVLGSFMPLETTMSAPAMGPAVSMGTSPSLSMHTQPQMNSLDFIMGVNEQPAAYGSNVAPDYNNQQWGSTNPMVPSLSNGDQPNSSIYDFLTGNRPRSADPYKDKPQQFLNXNNSGNNNNGVDAAGTAFHNSSSKYPNGNESFGNGASSSKNGGNSFMGKKLGIKNFLSGMKSGSSSSSSGPQQSSSDSSSSHTATVPSSASSANAGNQNQAMQPSNLTDVELLQNIPMWLKSLRLHKYTENLXQYNWEELTAMSDADLQKNGIAALGARNKLLKAFKIVLDCKEQGLIAKEAYEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S049</accession>
    <name>A0A1E5S049_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g146</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5S049"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="375"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ92388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="443" mass="48445" checksum="89678178AC207192" modified="2017-01-18" version="1">MEGVIVLDKTPKFLVASLYYNYIAPLIGYAIWPLHAACVLVVKCVRYLSVFPLLAKILVRGICTGAFQVVFWVLWKPLGIFLEFVQVLTKLLFDFVFFPVNVVLFVFLGTSLSKILAGAQSLDKKDYMIIGELLKIFLIHWSALLFLGVVLGSLNSLVMFKLLNSVFNISFLKFEFTFKISTLLKYVTGPVRSTLKIVEKVASLVKNEISNLGLQDPSPIQSTPLPSSPQASIGSLESVSKLPLDKQTAKPAAXTEHHAQTSPLLERPLGVSLKSESALSSRDSVSSISPGKRVLSPSSSKANRKHSIHATEPIISLNSTTGGVAIRRMSSTREELATQLPKDYFQQLQTPANSDTEKEGPEIERTSVQKLPTYSSKYSGSQSSHSELGEDEEAEFDDASESVFSYSXEETARTKVGTNGGSAKTSGAFVPRIHAANVIXKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S085</accession>
    <name>A0A1E5S085_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA damage checkpoint control protein RAD17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g235</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the rad1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5S085"/>
    <dbReference type="FunCoup" id="A0A1E5S085">
        <property type="interactions" value="159"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S085"/>
    <dbReference type="InParanoid" id="A0A1E5S085"/>
    <dbReference type="OrthoDB" id="337581at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030896">
        <property type="term" value="C:checkpoint clamp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003021">
        <property type="entry name" value="Rad1_Rec1_Rad17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10870">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10870:SF0">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02144">
        <property type="entry name" value="Rad1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01245">
        <property type="entry name" value="RAD1REC1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="380"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="399"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ92469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="422" mass="47985" checksum="EB87D1B628BA27A7" modified="2017-01-18" version="1">MQHTSKRNNTTRELLLFMSLTNDILFTATTVHLEHITTALNCLVPFGAKEDVLIIIDEEGLSFASEKSHIIKIQLFLSKDLFTLYNFDSSKAPFEKVCVKLNHILDSVNVANNVNNSGTGGSSNSNNNNNNNNNDETDFLECTLSYDGDGTPFVFIFEDALITERMEYSTYLIKEMDVFDILQIDLDRIEYECIIQGDILFNTLQDLKELDCKECYLYVSAKKNNKPHFSFIFKNEQLGLSRISLPSERTCLEKVEFMNNADGQRDQSAPQEESISYFDFQMLDKISKSVKIASKVLFRKDTRGVLSVNILSKTNNIIPVGNFRKSSATLQNRKKTNVLPKNYSGIVIDITMIEKESESGFQDIIEMVNRDENTISTKIRASRKRRRSDEQILDDEDSQNANTTSVNLGPTQNKKPGVPLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0F8</accession>
    <name>A0A1E5R0F8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutaredoxin-8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4508</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0F8"/>
    <dbReference type="FunCoup" id="A0A1E5R0F8">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0F8"/>
    <dbReference type="InParanoid" id="A0A1E5R0F8"/>
    <dbReference type="OrthoDB" id="418495at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="1">
        <location>
            <begin position="23"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="111" mass="12960" checksum="7A87D7398BE1822C" modified="2017-01-18" version="1">MSSKLDIARDLVSKNKFFQLTAAWCPDCVYTYRIYDKFGIKSKFAFFDVADLDRRSREYQEYRDAFTKVAGGVTNLPTVFVNGKFWATEAEFLKWEKNGTLEQEFKKVGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0R8</accession>
    <name>A0A1E5R0R8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Riboflavin synthase</fullName>
            <ecNumber evidence="3">2.5.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g4545</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the dismutation of two molecules of 6,7-dimethyl-8-ribityllumazine, resulting in the formation of riboflavin and 5-amino-6-(D-ribitylamino)uracil.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.9" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0R8">
        <property type="interactions" value="168"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0R8"/>
    <dbReference type="InParanoid" id="A0A1E5R0R8"/>
    <dbReference type="OrthoDB" id="10258924at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004746">
        <property type="term" value="F:riboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00402">
        <property type="entry name" value="Riboflavin_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000004">
        <property type="entry name" value="Riboflavin synthase, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001783">
        <property type="entry name" value="Lumazine-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026017">
        <property type="entry name" value="Lumazine-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006767">
        <property type="entry name" value="PRK09289.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00187">
        <property type="entry name" value="ribE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21098:SF0">
        <property type="entry name" value="RIBOFLAVIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21098">
        <property type="entry name" value="RIBOFLAVIN SYNTHASE ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00677">
        <property type="entry name" value="Lum_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000498">
        <property type="entry name" value="Riboflavin_syn_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51177">
        <property type="entry name" value="LUMAZINE_BIND"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="Lumazine-binding" evidence="8">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Lumazine-binding" evidence="9">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="repeat" description="Lumazine-binding" evidence="8">
        <location>
            <begin position="107"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Lumazine-binding" evidence="9">
        <location>
            <begin position="107"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="214" mass="24092" checksum="F50A6597623734E3" modified="2017-01-18" version="1">MFTGIVETQGTISKVQNDXDNFIVTINVTGTDFLSDCHVGDSISCCGICLTVIEFSNESTTESAWFKVGISQETLRRTSIEKMWKEKSKLNLERAMGMGNHRFGGHYVQGHVDTVANVIEKDSEGDSINHGFQLRDKEYEEFIVEKGFITVDGASLTVTKVEPEGNFWISMIKHTQEVLVPREIGDQVNIEVDLTGKLIAKQVKLYMDKYMKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R172</accession>
    <name>A0A1E5R172_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein mL49</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4268</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL49 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R172">
        <property type="interactions" value="160"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R172"/>
    <dbReference type="InParanoid" id="A0A1E5R172"/>
    <dbReference type="OrthoDB" id="19439at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007740">
        <property type="entry name" value="Ribosomal_mL49"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13477:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13477">
        <property type="entry name" value="MITOCHONDRIAL 39S RIBOSOMAL PROTEIN L49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05046">
        <property type="entry name" value="Img2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 7" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="164" mass="18481" checksum="E70498622F7095E1" modified="2017-01-18" version="1">MLNMRTMLRGSIKPTTLLSKPQCTYKLQSTIAANAXSSIPHEHAHTAPDLAAEESADTSDFKIFPSMHDVSVNDLIGSDRFTSSNYFIERSSQGNLPVYTDYKNGITYTEIRKIRGNSVKLRDDLINLMPYVNRKHIKVVPQSNKILIQGDHSTRIKKLLSTTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1D9</accession>
    <name>A0A1E5R1D9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1D9">
        <property type="interactions" value="87"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R1D9"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="263" mass="29410" checksum="3CF73929C1018D1C" modified="2017-01-18" version="1">MSLNKKSKEVFKEQHATFQYNRLHELPQALASTNKDQDGLDAVQSFNLSSVLGPLYLTKFFRSCKKNKIILPGETRGAILKTKKLKKWDKYEQDIKGKSXDNSAKSPRSNDGTTKFCEKCGNLRVYGSTTKVRTLKKNEDFFVELHCLLCDSKALFNASDPPTVWPTTTTNDPSVKETTPVVQGSNTPKNVLNKLYIGTPSPPPQGSSLTSNKVSKPVNKNISAKQRSKKRKTNTLADVLKQKKLKEENKSKSSLSLADFLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1K8</accession>
    <name>A0A1E5R1K8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4342</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R1K8"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="234"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="544" mass="62361" checksum="C958B578720959E7" modified="2017-01-18" version="1">MSLVGDKTGSMEVXNHISKVNGLFQDKTLAEIEEYLQDTINDELVVLGNQLYTQMQDQSLINEVILETCSVYGDIYNTNCNDIEXNLMSLCFDRDDADENNKSLGDTSLDIIPMDNRAFKEQDNTXMLDNIFNSLSTTRFDYDLELKLEEFTKKRDNXDNAESIDKFKTFNELYPKMISFDNRDKDNDNTTQTKQRADIFDKYYGKFCETLESLDVEQYTTGMDRDNDFQNTTSXSSSSSSSSSGNKNNTLLTVNMFIVKMVSLYQCINQESLNKKYKQLLSIVSMQLVRIYHYIDVNDLSYTFNNAYVNIKKLLIGKNEEEKQLANHNVSLRQMYVNFIKDEIVSLSKKLAMNXLKRDVKVLDFYHTTTLXEWENGHFTDTNNKSDIDSKCDSYSGDSYAGGNDIHNLYHDIEHYYTNGWYRPDMIDIGTKLNYIDQLFHQLKMLTVTGSTTGSATDDLCAFQLDIYKSVLDLPSYKKQTQDXGLLSSPFATLSTKPSPDTTISNDMITNGAINTDNLIKSITTHSNNHRWNSYLDSLCKDVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1S3</accession>
    <name>A0A1E5R1S3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="11">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g4183</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Essential for the maintenance of cell size and integrity in response to osmotic stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 16">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 16">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PPP phosphatase family. PP-Z subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="11"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1S3">
        <property type="interactions" value="378"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1S3"/>
    <dbReference type="InParanoid" id="A0A1E5R1S3"/>
    <dbReference type="OrthoDB" id="3972427at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07414">
        <property type="entry name" value="MPP_PP1_PPKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000006">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF484">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE PP-Z1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="13">
        <location>
            <begin position="734"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="504"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="943"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="58"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="252"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="321"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="361"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="402"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="424"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="456"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="515"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ80855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="980" mass="107331" checksum="F29E589047899DB0" modified="2017-01-18" version="1">MGNSSSKPSSGSSSKSSKLNKSSSSNKKTGSSNISSNTSTGSTGKKTGLLNKRKGTVQPASGLSTETAPQPAQKTVNTISSNNRETHKNVSQAGNSNIHSTSKDHYPTPNNDKIKQHSTTEQGQRPIDKDAFVNSTADSAVNAAHDRTSNTTEPSKSYQRHKRSKSTGSIPSSEISAEVAKHKSKTPTMASKIEVTKMKNFANVDXPSEQEIKDKYGEDDEDDDEEDDEDDEDEDEEASTRHSQEKLANRTSSSSSKKSGSIFNKRPSSSSATLSPPPLSKASSFSKSVKSSRKSSFDQTLPPSMVTIKPSTSILKKNPSRHSMSSSAYYSDNALTDDETDPSTYHGNNRRSFDSSRRHLSSSSRRSSSTSVVSGTTGNMMNNGAGNNATNSPYNSARDRNSSFGDNNDFQNLADPLSRTSTRNSGNSVSNNTINSTGSGSRIISSTGNGGNLMGSRKSSFSSLKSTGXTAYSTPAGSPGIEMYQSGKAGEGDYFNMNEHAPNNEGGNTIPGAYSQISGHSNGEQQVAEGETSHHPKYAHSHVEPDLSQLDPEERELFLAQMQRVDGSVNNASNDHLEGSSQTHHRYSKQYGAEDLARTADLGSSGIEKKTIKRPLKPLNVDXTIQKLLDAGYSGKRTKNLVLKNSEIQQICSIARDIFLQQPPLLELSPPVKIVGDVHGQFQDLIRMFDKCGFPPSANYLFLGDYVDRGKQSLETILLLLCFKIKYPENFFLLRGNHECASVTRVYGFYDECKRRCNIKTWKLFVDTFNTLPLAAIVAGKIFCVHGGLSPVLNSMDEIRHVSRPTDVPDFGLINDLLWSDPTDSINEWEDNERGVSYCYNKVAINKFLNRFGFDLVCRAHMVVEDGYEFFNAHTLVTVFSAPNYCGEFDNWGAVMSVSDALLCSFELLEPLDSAALKQVMKKGRQERKQAALQAAEFEQQELQELQQQQQTEDRVLGNYRAQDAVREKNEIGQPEPTQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1W3</accession>
    <name>A0A1E5R1W3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14 15">Diphosphomevalonate decarboxylase</fullName>
            <ecNumber evidence="3 14 15">4.1.1.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g4293</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(R)-5-diphosphomevalonate + ATP = isopentenyl diphosphate + ADP + phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:23732"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57557"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.1.1.33"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:23733"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 15">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14 15">Belongs to the diphosphomevalonate decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.33" evidence="3 14 15"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1W3">
        <property type="interactions" value="676"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1W3"/>
    <dbReference type="InParanoid" id="A0A1E5R1W3"/>
    <dbReference type="OrthoDB" id="10253702at2759"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00100"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004163">
        <property type="term" value="F:diphosphomevalonate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000018">
        <property type="entry name" value="Diphosphomevalonate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.890:FF:000005">
        <property type="entry name" value="Diphosphomevalonate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005935">
        <property type="entry name" value="Mev_decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029765">
        <property type="entry name" value="Mev_diP_decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053859">
        <property type="entry name" value="MVD-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041431">
        <property type="entry name" value="Mvd1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01240">
        <property type="entry name" value="mevDPdecarb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10977">
        <property type="entry name" value="DIPHOSPHOMEVALONATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10977:SF3">
        <property type="entry name" value="DIPHOSPHOMEVALONATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18376">
        <property type="entry name" value="MDD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22700">
        <property type="entry name" value="MVD-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015950">
        <property type="entry name" value="Mev_P_decrbx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 15" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 14 15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 14 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="11 15" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="8 15" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="10 15" id="KW-1207">Sterol metabolism</keyword>
    <feature type="domain" description="Diphosphomevalonate decarboxylase-like N-terminal" evidence="17">
        <location>
            <begin position="11"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Mvd1 C-terminal" evidence="16">
        <location>
            <begin position="198"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR015950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF18376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF22700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ80896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="397" mass="44035" checksum="E37F4267B8EFAE22" modified="2017-01-18" version="1">MSEVYTSSITAPVNIATLKYWGKRDKSLNLPTNSSISVTLSQDDLRTLTSVGTGPSFTKDRLWLNGSEDLKLSESARLQQCFVELRKLRKNLEAKDATLPKFSEWKLHIVSENNFPTAAGLASSAAGFAALVTCIAKLYELPESYSEISKIARMGSGSACRSLFGGYVAWEMGVEKNGSDSKAVEVAPMEHWPQMKACILVACADKKDTPSTSGMQLTVATSDLFKERIXNVVPARFEQMKKSILDKNFAEFAELTMKDSNSFHATCLDSFPPIFYMNDTSKKVIKLCHLINDYYGETIVAYTFDAGPNAVLYYLEENEEKLMKFIYNLFKQLPGWDTKYSAAXLQKFVDADKNELAALNLDFELYKGCTRVILTQVGPGPQATTSTLINKTTGLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R274</accession>
    <name>A0A1E5R274_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATP-dependent RNA helicase DRS1</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">ATP-dependent RNA helicase drs1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">AWRI3579_g3879</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-binding RNA helicase involved in ribosome assembly.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 24">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DEAD box helicase family. DDX27/DRS1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R274">
        <property type="interactions" value="939"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R274"/>
    <dbReference type="InParanoid" id="A0A1E5R274"/>
    <dbReference type="OrthoDB" id="10259843at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17947">
        <property type="entry name" value="DEADc_DDX27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000842">
        <property type="entry name" value="ATP-dependent RNA helicase DRS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 17 22" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="21">
        <location>
            <begin position="271"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="19">
        <location>
            <begin position="302"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="20">
        <location>
            <begin position="506"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="113"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="700"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="16">
        <location>
            <begin position="271"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="61"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="118"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="204"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="231"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="700"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="761"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="774"/>
            <end position="787"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00043881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="OEJ80992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="787" mass="88300" checksum="F57C3EE06AC54E8B" modified="2017-01-18" version="1">MGVKKSSAKETSKQKFDLDFVPTISDSEEDVPDLEVLSDADGNQDEEGTTNGTTXSESLKTAKKSKKSKKPKKSKKATSVEEVDADGQTDLNADFNFNLDEDDLYSTKEELQSWNFEGDDKKNSTFKDIDLDGIIKRKGGLIQLAGIADDGEAESEEEKEEEEEEEEEEEKEQQPESTPKNEKDDDELAMDGFGMGTQNQNANGEDEQDNNEEEEEEEEEEDKSSDEEGTEELKVDGKLKEVKEEEDSAEAIAQFYATADEASAAKEQTHKTFNSLQLSRPVLKGLSALGYSKPSPIQGASIPIALLGKDIIAGAVTGSGKTAAFMIPIIERLLYKPTHVPATRVIVLTPTRELAIQISDVGKKIAKYVEGISLGLAVGGLNLKQQEQQLKTRPDIVIATPGRFIDHIRNSASFSVDSVEILVMDEADRMLEDGFQDELNEIMSLLPQKRQTMLFSATMNSKIKQLISLSLKKPVRIMIDPPKQAADKLTQEFVRIRKREALKPALLFQLLKKLDSTGQTRIVVFVSRKETAHRLRIILGLLGLKVAELHGSLTQEQRLESVTQFKSLQVPVLICTDLASRGLDIPKIEVVINFDMPKTYEIYLHRVGRTARAGREGRSISFVGESTLDRLIVKSAIKSVSENVSKSRGKALGRNVNWEEVEKLNKLLEQKGDVITEILDEEKQEKEILRAEMEIKKGENMLKHKHEIQARPKRTWFQSEKDKKNSQVIQALSSNKKTLNSKKRKQQEAKEDGYSRSYKKTQNDRIADQERSFKKQKKTDGKKKGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2B7</accession>
    <name>A0A1E5R2B7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribonuclease P protein subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g4040</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal RNase P protein component 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2B7"/>
    <dbReference type="FunCoup" id="A0A1E5R2B7">
        <property type="interactions" value="240"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2B7"/>
    <dbReference type="InParanoid" id="A0A1E5R2B7"/>
    <dbReference type="OrthoDB" id="124041at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.210">
        <property type="entry name" value="Ribonuclease P/MRP, subunit p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016848">
        <property type="entry name" value="RNase_P/MRP_Rpp29-subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036980">
        <property type="entry name" value="RNase_P/MRP_Rpp29_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023534">
        <property type="entry name" value="Rof/RNase_P-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002730">
        <property type="entry name" value="Rpp29/RNP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01868">
        <property type="entry name" value="RNase_P-MRP_p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027081">
        <property type="entry name" value="RNase_P/MRP_p29_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00538">
        <property type="entry name" value="POP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101744">
        <property type="entry name" value="Rof/RNase P subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR027081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ81039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="287" mass="32725" checksum="30704FE5DC1D1952" modified="2017-01-18" version="1">MDKVQPFIRDYLLSRGEKDVASIEDRLNESLIISATKKRIKKPRKLKSLLHVSHGSSKHSLKQTSYKSVNANSKAILRSYVLSSKHAVERATRLINKKEAEGSITTLSKEEKYQYLKENYPDIYRQLPKKFTDVQETLYKKMWLPYIREVLKISDVESLQCTNLQNCLLKLSIADYNGCLLKVSTSRNTSTLNTSGIVLWDAQKSFIMLSPKKDGFDEVKVIPKKGTLFEISIPLETSNGTGDSAIFNIVGDRFQYRSSDRSGRKFKNRRVDDLLFYLSSEATSSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2D3</accession>
    <name>A0A1E5R2D3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Urea active transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4325</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2D3"/>
    <dbReference type="FunCoup" id="A0A1E5R2D3">
        <property type="interactions" value="830"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2D3"/>
    <dbReference type="InParanoid" id="A0A1E5R2D3"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015489">
        <property type="term" value="F:putrescine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015606">
        <property type="term" value="F:spermidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015204">
        <property type="term" value="F:urea transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11476">
        <property type="entry name" value="SLC5sbd_DUR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031155">
        <property type="entry name" value="DUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154:SF4">
        <property type="entry name" value="UREA ACTIVE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="58"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="362"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="405"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="433"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="462"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="500"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="624"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="655"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="741" mass="81519" checksum="453A078263A81611" modified="2017-01-18" version="1">MSSSSDINRILTPNDGYAIVVGFTALFAVSMILISYCLKRYSKEIMTSEEFSTAGRRIRTFLGVTSIISSWTWLSTLSTSSSLTYQNGIFNAYTYAGGAALQLFVFCLVAVTVKLKAPSCHTYLEIIKVRYGPTAHKVYMFFALATNILVTVMLITGAIDSFNALTGANTVGLGFLLTLGVIIYTMFGGLKSTFMGDYTHTIAVVIIILTFLFKVYCTSPLLGSPGKVYDLLKESTEKYPIAGNAEGAFLTFKSRSAGMFFVINIAGNFGTVFLDNSYWNKAISTKAIDGSVFWCYIIGGVCWFFIPLSISTALGAACRALENTPAFPTYPNRMSSDQVAAGFVVPFAAQTLLGKSGAYAALILYISAFCSAFSGELVAVSSISSYDIYQGYINPKATGKQLIRVTHLSCVIFAGCLIGFGIGLHYANVGMGYIYELMGVIISSAVIPATATIFWKDMNKQAVIFTPIIGTGLAIMSWLACTSAEYNHVISVDTTFADNPMLVGNVVALLSPLVFIPIFQLCFGKQNFDWDILKNAITRVSEIEEIEAQDGEVDQDGNVAQDVIKADAKEGPAESSSSSSNHQQDIEKLKPVVSVASAAFEVPQTEIDDMRHLEEIALTKYKKWATYIAWAAAIIFCVLWPMPMYGTGYVWSKQFFTGWVCVMFIFMFITGFVVIIGPIYEGREDIYYTFRGIYWDLTGQSQKLYQWQEENPEKLHVVQSQIYNRAFEKDEDVQNLDDVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2F6</accession>
    <name>A0A1E5R2F6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RP41</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">S18</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">YS12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g4223</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2F6"/>
    <dbReference type="FunCoup" id="A0A1E5R2F6">
        <property type="interactions" value="1194"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2F6"/>
    <dbReference type="InParanoid" id="A0A1E5R2F6"/>
    <dbReference type="OrthoDB" id="10254436at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00364">
        <property type="entry name" value="Ribosomal_uS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.1000:FF:000001">
        <property type="entry name" value="40S ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_uS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028333">
        <property type="entry name" value="Ribosomal_uS17_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019979">
        <property type="entry name" value="Ribosomal_uS17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032440">
        <property type="entry name" value="Ribosomal_uS17_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006345">
        <property type="entry name" value="PRK08572.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03630">
        <property type="entry name" value="uS17_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744:SF9">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16205">
        <property type="entry name" value="Ribosomal_S17_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00973">
        <property type="entry name" value="RIBOSOMALS17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00056">
        <property type="entry name" value="RIBOSOMAL_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS17 N-terminal" evidence="9">
        <location>
            <begin position="8"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00075870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00077551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00080769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ81079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="156" mass="17986" checksum="1F3D435B2B1BC376" modified="2017-01-18" version="1">MSTELNVQSERAFQKQPHIFTNPKAKTNRRTKRWYKNVGLGFKTPKEAIEGHYIDKKCPFVGDVSIRGKILTGTVVSTKMHRTIVIRREYLHYVTKYNRYEKRHKNVPVHVSPAFRVQVGDIVTVGQCRPISKTVRFNVLKVAAATGKVNKKFAKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4J4</accession>
    <name>A0A1E5R4J4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Vesicular-fusion protein SEC17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3554</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SNAP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4J4"/>
    <dbReference type="FunCoup" id="A0A1E5R4J4">
        <property type="interactions" value="903"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4J4"/>
    <dbReference type="InParanoid" id="A0A1E5R4J4"/>
    <dbReference type="OrthoDB" id="9984275at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005483">
        <property type="term" value="F:soluble NSF attachment protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035494">
        <property type="term" value="P:SNARE complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15832">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000049">
        <property type="entry name" value="Alpha-soluble NSF attachment protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000744">
        <property type="entry name" value="NSF_attach"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768:SF8">
        <property type="entry name" value="ALPHA-SOLUBLE NSF ATTACHMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768">
        <property type="entry name" value="SOLUBLE NSF ATTACHMENT PROTEIN SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14938">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00448">
        <property type="entry name" value="NSFATTACHMNT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ81837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="291" mass="32556" checksum="58CE160D533BCE64" modified="2017-01-18" version="1">MSDPLELIKQAEKKAQPTTGFFKFMSNDADKFEEAADLYVQAANSYRVSKQVGSAGECFMKAAECQLKAGNDNEAGNTFVEAFKMLKSFEPSKAAESLDKAISIFTAKTGQFRRAANYQFDLGELYETSLNDFPKAIAAYEAAGEWYGQDSATSLSNKSYLKCADLKALDNQFFEAVQIYNKVVENSLGNRLSAWSLKEYYLKIGLCHLAATDAVAAKKTLQDAQRNTDDPNCREYQLLSTLIDCYNEQDAEKLSAAVFDFDRFSKLDNWKTTILLKIKDSLISQEDDDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4K0</accession>
    <name>A0A1E5R4K0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Putative ferric reductase transmembrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AWRI3579_g3553</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ferric reductase (FRE) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4K0">
        <property type="interactions" value="399"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R4K0"/>
    <dbReference type="OrthoDB" id="4494341at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000293">
        <property type="term" value="F:ferric-chelate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF9">
        <property type="entry name" value="FERRIC REDUCTASE TRANSMEMBRANE COMPONENT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <keyword evidence="7 17 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184601" evidence="18">
        <location>
            <begin position="23"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="221"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="273"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="346"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="375"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="19">
        <location>
            <begin position="409"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="20">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OEJ81839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="714" mass="81706" checksum="2BE99D1BFE3158EE" modified="2017-01-18" version="1">MRLNRFLSHIAILLSICHLSFAQLASAWWKANQLSVGRACKTAVTNAAIYCKGDNTVEYDCQCADVVAMGAYAFCGYTETEGHPASRKRFEDYFISFCPNYTHQMIADSYANVTNYIKNSTEISGFNRTIPSRVPVLYSKARYTIVYKSVYQRFLNNDYGIIFGSALVGYWAAIFAVFAIYRCLEMMNLSDKLVNNNRVFRFFQANIFLKPVFGKKHKSDYIFGCVPVTADAIIIIGFIILDVVACFVNIHFLPNSTIWPVKATQIGRLIGDRSGYIAVFTTNLTWLFAGRNNFILWCTGWNFQSFLTYHKWVARMTVFNVLVHSIAYLINGKSMGLVAIREAQDWYRWGCVATVAGSVMLLQSMYPLRKHAYEVFLYFHIVLAVFFLVGTWIHLVWYEFQYYVYASVGLWCFDRLLRVVRIAAFGGFRKNNVKIVNNEFLILSIKNYNKKFFKPQPGNFVFIYFGLKNCFWQSHAFTILSASEEELKVIATVKKGCTKTVFSHMVAAKKXTMDMPIAFEGPYGSTREKIINHDQNILMYSTNTGVAGPYNYLKTFAKKGALLNKNVKFYWTVRHWTTLELLAEELQFLSEQNNIEVIVHVTQYDDAPXSSCEQSLSDEGKQTSAVEKVTSCQSSSFSADSIQKLYPNIKFRPGRLPVKDTVTRDISECLDNTNITIMLCGHPSFCDELRSIAIENIKKEKTKNIHLIDDLQVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4T7</accession>
    <name>A0A1E5R4T7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">37S ribosomal protein MRP10, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3709</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4T7">
        <property type="interactions" value="118"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4T7"/>
    <dbReference type="InParanoid" id="A0A1E5R4T7"/>
    <dbReference type="OrthoDB" id="2210at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010625">
        <property type="entry name" value="CHCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017264">
        <property type="entry name" value="Ribosomal_mS37_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28066">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN MRP10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28066:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06747">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="CHCH" evidence="2">
        <location>
            <begin position="31"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ81900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="95" mass="10883" checksum="9E959E25A95ECD2B" modified="2017-01-18" version="1">MANKAPPVYKLPPLPRIRVRRPVMKQEXNRCMVLMSNLLQCWASNGHMDSKCADLVKNLKDCTRDSPRGTEVEAKRSNINYHAARLHKRISGKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4U7</accession>
    <name>A0A1E5R4U7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Geranylgeranyl transferase type-2 subunit beta</fullName>
            <ecNumber evidence="3 11">2.5.1.60</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g3512</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the transfer of a geranylgeranyl moiety from geranylgeranyl diphosphate to both cysteines of proteins with the C-terminal sequence -XXCC, -XCXC and -CCXX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>geranylgeranyl diphosphate + L-cysteinyl-[protein] = S-geranylgeranyl-L-cysteinyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21240"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11537"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:86021"/>
            <dbReference type="EC" id="2.5.1.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the protein prenyltransferase subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.60" evidence="3 11"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4U7"/>
    <dbReference type="FunCoup" id="A0A1E5R4U7">
        <property type="interactions" value="308"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4U7"/>
    <dbReference type="InParanoid" id="A0A1E5R4U7"/>
    <dbReference type="OrthoDB" id="5428259at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004663">
        <property type="term" value="F:Rab geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02894">
        <property type="entry name" value="GGTase-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000012">
        <property type="entry name" value="Geranylgeranyl transferase type-2 subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026873">
        <property type="entry name" value="Ptb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF11">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-2 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 11" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 11 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="12">
        <location>
            <begin position="6"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ81910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="327" mass="36746" checksum="B92D286B7C1D4AE5" modified="2017-01-18" version="1">MSTPPFFREKHIAYVKSLDTKTQNYEYWLTEHLRLNGVYWGLTALCCLKSKDTFDKDEVIKFVLDCWDEENGSFAPFPKHDAHLLTTLSGVQILATYDSLHVLGKPKIDRIVEFVERNQNDDGSFQGDEFGEIDTRFSLNALATLSILGRLSKKVVDPAVDFIGRCYNFDGGFGLCPGSESHAAQVFTCLASLAIADKIHEVLSPEQLQNVEWYLAERQLESGGLNGRPSKLPDVCYSWWVLSSLAILGKLGSIDFAKLRDFILKAQDPDNGGISDRPDNEVDVFHTVFGVAGLSLMNYENLEPIDPIYCMPCAVTKNFKKYSVDYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R542</accession>
    <name>A0A1E5R542_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DASH complex subunit DAM1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Outer kinetochore protein DAM1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g3656</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="15">Component of the DASH complex consisting of ASK1, DAD1, DAD2, DAD3, DAD4, DAM1, DUO1, HSK3, SPC19 and SPC34, with a stoichiometry of one copy of each subunit per complex. Multiple DASH complexes oligomerize to form a ring that encircles spindle microtubules and organizes the rod-like NDC80 complexes of the outer kinetochore. DASH complex oligomerization strengthens microtubule attachments. Within the complex, DAM1 and DUO1 may form the microtubule connections. On cytoplasmic microtubules, DASH complexes appear to form patches instead of rings. Interacts with the outer kinetochore component NDC80; the interaction is direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex DAM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R542">
        <property type="interactions" value="194"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R542"/>
    <dbReference type="OrthoDB" id="5586015at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990537">
        <property type="term" value="C:mitotic spindle polar microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990758">
        <property type="term" value="P:mitotic sister chromatid biorientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013962">
        <property type="entry name" value="DASH_Dam1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28113">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28113:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08653">
        <property type="entry name" value="DASH_Dam1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="96"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="237"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="131"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="261"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="17">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ82027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="288" mass="32028" checksum="7145B5707C4E281C" modified="2017-01-18" version="1">MDLVLKSLENVSQQMENLDHNMTRLHFINESLSNFNESNGALVYGLMCNTWSIELQSNQFNVDXVQRELDNIEKIEEIENNIKNVQLEIEKLTTPLPKTDSALHTLPGKPGSSRKPANTSQSNAGRKRGEPTTSSSRPTHSLSNGSHRHIVTARASNNDTTSSFVTNPDASTIQPLKNYGSARHNMPNRAQPTPKSTADRVYKNRRKSSILNSAREKSIFQTPENHHTERYSLAVGPTRLGTMGXPQQRTSLAGPAHQSRETNASKNGRAQRYTTSGTEFRSARSXSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R568</accession>
    <name>A0A1E5R568_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3717</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R568"/>
    <dbReference type="FunCoup" id="A0A1E5R568">
        <property type="interactions" value="178"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R568"/>
    <dbReference type="InParanoid" id="A0A1E5R568"/>
    <dbReference type="OrthoDB" id="191150at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF9">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="305" mass="34866" checksum="D9E5B16F68CD0087" modified="2017-01-18" version="1">MFKKPLKNLLTARWYSPPRIPKTQFNQSQYGTSFQNSDPASNIGDLSFINRAMQSRIFTEPTIVIRREIEMMNLFLGYEQVNKYSVCDVEGFEIGRIDEDDSLGGRGFLNLINRQLFRTRRPFSLDIWDHTMPGVKLGSVNRGFKFINSHVQCHIGDTKSNDIVAGESRQVWAPLRRKYELYAVENPAESISPQDAALDQFGAIDTMFLGWDFRVQDEQGLVIGGCDRSFGGFGREIFTDTGIYILRMDSNQSFNGVEDPQRMNFNKILNFYERCALIANCISIDFDYFSRHSGPNGLVSTSSYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R662</accession>
    <name>A0A1E5R662_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein MON2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3772</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R662">
        <property type="interactions" value="772"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R662"/>
    <dbReference type="InParanoid" id="A0A1E5R662"/>
    <dbReference type="OrthoDB" id="294853at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032629">
        <property type="entry name" value="DCB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16213">
        <property type="entry name" value="DCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Mon2/Sec7/BIG1-like dimerisation and cyclophilin-binding" evidence="4">
        <location>
            <begin position="8"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Mon2/Sec7/BIG1-like HUS" evidence="3">
        <location>
            <begin position="209"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ82023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1601" mass="182632" checksum="639D498CEA8CA2C0" modified="2017-01-18" version="1">MNTFTFVDFRKKLSTDLAALTSDCKRKYPDVKNAAAKSVKILDQCSRFEDLKKNNDLVDPFILACATENVKLVSTAMLSYQRLAMEQCVTSVSKIDQILDSFLICTNLAVEIQLKVLQILPVFFKTYSAFIVGSSLKKMLCCFTNLLQPNKSSVVIGSSSATLQQLINGIFERPSLLQNDIEDGKESSDVQVFHTLIDNENKTIALNKYQXDTNVVFRDICMEQVPDSLLDFKXLTIDIVLEILESIFLNQKTVIIQRKDLQFILKTVAVPFLLKEISAPTTKSYAITVRSLRATKLLMDRDYIEILDLELEVVLSFLIHILQGDAATPPWKKTLVIEFFCNVEFLTWFWIFKYFDARKTEKQQVLLQLLVAFKEELLSEKFQPYLREAPILVNGEPPLLSQDSITMKTPLLELVDKVHPPVIDPKYGLILLFQIVTNLSSESESYTLKTAQNKEELDILKLMIAHIYDVLSESLRLFFYSSALDTNLFHRLVRGFQKLAHSCCLLKLKKQFDQSIQDFAKYIVQLTAESPKDYLEGEYQPDTQSLSSANTEISILGAISDSFLGNSSNNASNNDDNTSSVRFYPRSFHSRNLDLLKACVALFISSSTALDVSNWNVFFKIWQWCNYYLVGPSQLFSDMFLNKAVPENPFGLSKPSLLSLENSFARLFESTSXYSESDLFSITDSLISLSREALKDEKFGGSFSPFNSNGQLLIATYNRDFFIKILESVMSMNGNRIFRTFKDETWNRVFDYFIEESTTRKENLNQDXRLDFVQSFNAIIYGLISSAGSDPAQFALVENHVMTLLMRLINSMLSLKTDSSKDLYNGNVNIESDILLQTLKTVKDLVDNYGDYLKNSWTPIFQILGSPFSLISDSSYKDADSEEERLAIQEALKTKHKSMIQVSFSVFKLISDDFLQSLPLVEIKGVIQTLVQYVSQDTDLNVSFSSISYFWVVGDYLRSSESSANSNETFGALLDGEHYNSLIQKHXSLLSLLSSENLTKSETYTTLWLFLLQSLINCSQDKRLEVKNGAIKTFFQIIDSHSSFLPSWNLVFEEILVPFLGSTHANIPPDFADITLKGLLQVYDLYFNNLPPVTLDNTPKWLKLLDFVACCLRSTNYDTXFTGFDNLKKVLESCEKQEFSGDFVDRCYITWKQYEIDYTDFSQSEKKTNYDCIDKMLDCYPSMHKLLLKNDMLTVEKIVSTFQVFNQSVKYPLLPPFASDKQFLSNLQKSLMNALSCFANVQDSAVSIMYLNQLAVISALQFGTKDTIQKKLGSKLPNNAKSRIPTFEAVGYEAAKTLNENILQLTKIEESLLSSNKYLTSTLVILSEQIISKNWLDFTKEKTAPLWVMASKTFHKLCVLIFSSESTSAKKTFVESLVPLIIDVMSALLTKTTAENPKNELYTREEYSSYKVLFMDNMCFFDNDSFEKLMSSIWSRSFFYEMDEVENNILSSSKNLENFIEKLVIFDFENTLGSIEEPEVIPSFELSKLALEDLIFFSSNKTLKNTCRSFLISRVLIVLRKYISDQKLRFKKPVSTVNKLEILWVLEGLFKVCSSFKSTSDFDGLGVLYPFVLRTIPASNQFEDLQLILQKLSLIFAEQNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7G1</accession>
    <name>A0A1E5R7G1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RP21</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">S13</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">YP28</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">YS11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g3303</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. uS4 is involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes). Interacts with snoRNA U3. uS11 interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7G1"/>
    <dbReference type="FunCoup" id="A0A1E5R7G1">
        <property type="interactions" value="1125"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7G1"/>
    <dbReference type="InParanoid" id="A0A1E5R7G1"/>
    <dbReference type="OrthoDB" id="1697570at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000021">
        <property type="entry name" value="40S ribosomal protein S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_uS4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005710">
        <property type="entry name" value="Ribosomal_uS4_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003139">
        <property type="entry name" value="PRK04051.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01018">
        <property type="entry name" value="uS4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF5">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 13 16" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 12" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS4 N-terminal" evidence="15">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="145"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00060032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00075843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00077547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00080764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00081686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ82821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="179" mass="20523" checksum="F755CB83E9C27A30" modified="2017-01-18" version="1">MIERPYESARLDAELKLAGEYGLKNKKEIYRITFQLSKIRRAARDLLTKDEKDPKRLFEGNALIRRLVRVGVLDEDKKKLDYVLALKIEDFLERRLQTQVYKLGLAKSVHHARVLITQRHIAVGKQVVNVPSFMVRLDSEKHIDFSPRSPFGSGRPGRVARKRAAAKAESGEAAEEDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7G5</accession>
    <name>A0A1E5R7G5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MED8</name>
        <name evidence="10" type="ORF">AWRI3579_g3300</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7G5">
        <property type="interactions" value="168"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7G5"/>
    <dbReference type="InParanoid" id="A0A1E5R7G5"/>
    <dbReference type="OrthoDB" id="5329317at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019364">
        <property type="entry name" value="Mediatior_Med8_fun/met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13074">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13074:SF9">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10232">
        <property type="entry name" value="Med8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ82818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="205" mass="23688" checksum="2C4E734ABB550CB5" modified="2017-01-18" version="1">MVNQIVENYDYAAVPAQELDAIRMKLSQLTSSLSKIKEDIGKPIGFQQWHSLNAKITLLLTQLXSLSRTLQHYEYTLNSLLIYPLPNFPTTAHESLLTTLLRKKNIPEVDEWIKDCKIETSSAQAQQERIREDDSLSKWCLSALKKERTKIFNADNQNEETNDYDMDIDSDFNANKPLKKDIFKILPPPFDVNNVMKYMYTGEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7K2</accession>
    <name>A0A1E5R7K2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7K2">
        <property type="interactions" value="122"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7K2"/>
    <dbReference type="InParanoid" id="A0A1E5R7K2"/>
    <dbReference type="OrthoDB" id="4067613at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="522"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="567"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="722"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="838"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="997"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="85"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="412"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="530"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="570"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="610"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="663"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="725"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="838"/>
            <end position="848"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1052" mass="117002" checksum="A54EB4AC7FC1C864" modified="2017-01-18" version="1">MSEHKRRSFFFGLNKSSSDDQSTPAQQQTRQLPKKTTHPPPAQIHAKNTNPFVTANSSNHRKENGSSFKTFSVVRADSTAQDLRNANNNSNQLQQQQVLRPDLNRSSSSSYTTYTNANVTAKKSMANAPSLPSTAVPIVAPTFTSRASSMSRPPPPPVDMVFVRNXSNKSDKTLTGVPSSLDPATRSSSVYTSTTGNMSPNVEKNLPQFTNQRKASHADRMREFSDASAFSNQSSQLRNPMKNHKRERSQVEIYIDQLDSFMKDEEVKKHAGAYQDDDSENLSAQLEGNRAXNPFYDENFKXLNKDQASRTKSKEHNDFSANHSNDENSFDNASVQNDHFSPVKPLYKKDNSGTFSQYVEPFEMEPPFLNSTLSNLALDSPSKDPQNDSFHTAQSNDDSLENESDQLHLSGKHSSLNSARSNDYINNQFSPDNPFAGDNKPLYPEPIRLINTNSSEDYDNHAQENNGFDQYSFDNSIVESQLPRKHESVKEMQNVSIIHSSSDEMLGSERKEQRFRVINQDRPSFYVNHDEDDDEDDVDDGAAGKAYYGHKDAASFTYQXSEHTPPLYYKSSTNSTSSLEYDQDEHESSLLDADSALKTSMHSERADSNIGRNSFISNSSFPAKADSDQKFSNGRPRLXTQQISTASFKFPSHGTTEDHEINGETSETTSSLLXHDTPKSXQQSKYKPFVETERNPHLVDQESFSSDHYVPAIPETPIAPQIPESVLSSSSGDTSGQLDSSNLSXVTRSATLTTGALRDTGGKISLVSEYVEELRYKYFPTANVVQPPPNLPLSIKSKNTLELPQSSNIKVKIRTSSKQIGIKHGKAKQKLLSLEAPAEEDEYSDVEDPXNISFNGNEGYIPTAEEEEERLILSELPGDEAYNGDDLMAPLRERNKTINSAKNGNARVSRSDSTKSYFTKNAHRFRSGTLDNSYVPPMGNFGKMGALPQLPSNQAIPLYKENVITDPVTPTSSNMSGXNKNSLNDDEVMKEIEKTYRKPTNDFGKSGLSNXNTNESKTRTNKHGSLVSADFYHQDLNATGSSLKVANPDHEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7L1</accession>
    <name>A0A1E5R7L1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Succinate dehydrogenase [ubiquinone] cytochrome b small subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3141</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion inner membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the CybS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7L1"/>
    <dbReference type="FunCoup" id="A0A1E5R7L1">
        <property type="interactions" value="251"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7L1"/>
    <dbReference type="InParanoid" id="A0A1E5R7L1"/>
    <dbReference type="OrthoDB" id="18577at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048039">
        <property type="term" value="F:ubiquinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03496">
        <property type="entry name" value="SQR_TypeC_CybS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase, transmembrane subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007992">
        <property type="entry name" value="CybS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13337:SF5">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEIN SHH4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13337">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05328">
        <property type="entry name" value="CybS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0830">Ubiquinone</keyword>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <note>ligand shared with SDHC</note>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>a ubiquinone</name>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <note>ligand shared with IP/SDHB</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR607992-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR607992-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ82900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="180" mass="19624" checksum="B11B85DDDD47E58F" modified="2017-01-18" version="1">MFARNLIRRQVGLVAKQHQHQQLFHKSAVNKLTIPFLSTLPQNAGGVKGDVNSNLTPPKSDKMHGGIHWDFEKILSIGLVPLCGASLASGGDISGVADTLLSGILLAHVWVGFQSCIIDYIPARVYGKNHNYAMYLLSLGSLVSVYGIYELETKENGLIGVVKKLWGTPGQDSEIKEEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R815</accession>
    <name>A0A1E5R815_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein ROT1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R815"/>
    <dbReference type="InParanoid" id="A0A1E5R815"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184666" description="Protein ROT1" evidence="1">
        <location>
            <begin position="21"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="236" mass="26471" checksum="6011997ADAC11979" modified="2017-01-18" version="1">MNIVLAKALFVSVFFYQTLCAYANKNLLENNLSISGTLLGDSMWKNNFHGTPFLLNTHTDCLKNQTFRNTWYVSTNSKLVKLAVRQGNAEQVGTVAERESLITKKSIVSFSGPPHIAPVLLPMQAATYINGVANYETHPQNFKLQILSNNAYPSNAKIKLRGRSASGAKPFYKVTRNTTRLQHSSYFNVNPKKTNYGWEIKPKENSAFSTRNLASGKYTIFGLGFYAIIYLIEVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R824</accession>
    <name>A0A1E5R824_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">tRNA dimethylallyltransferase</fullName>
            <ecNumber evidence="5 6">2.5.1.75</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3473</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>adenosine(37) in tRNA + dimethylallyl diphosphate = N(6)-dimethylallyladenosine(37) in tRNA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:26482"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10375"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74415"/>
            <dbReference type="EC" id="2.5.1.75"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 7">Belongs to the IPP transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.75" evidence="5 6"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R824"/>
    <dbReference type="FunCoup" id="A0A1E5R824">
        <property type="interactions" value="984"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R824"/>
    <dbReference type="InParanoid" id="A0A1E5R824"/>
    <dbReference type="OrthoDB" id="775260at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052381">
        <property type="term" value="F:tRNA dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00185">
        <property type="entry name" value="IPP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039657">
        <property type="entry name" value="Dimethylallyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030666">
        <property type="entry name" value="IPP_transferase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018022">
        <property type="entry name" value="IPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00174">
        <property type="entry name" value="miaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088:SF89">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01715">
        <property type="entry name" value="IPPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039110">
        <property type="entry name" value="IPP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR039110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ83038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="447" mass="51695" checksum="7ACBC0CE8BF4E11F" modified="2017-01-18" version="1">MASVIPAIARKKIIVIVGTTGVGKSQLSIDLASKFNGEVINSDSMQVYKHVSTVNNKHPLEERNGIPHHVMDLIDLDDDYNIHKFEKDCLQSIRSIHSRGKLPIVVGGTHYYLQSLFDKKIDQDYNSDLIDPNHKLFLEENDSNYKVLYERLCQIDPYVSTKFHPNDGRRIKRMLEIFYSTGQKPSDIFTNQKFNLKFDTLFLWVYSNPAELSARLDLRVDKMMKVGSGGLEDVQLLYKLYQHGKMTPQSLDHGIWQVIGFKEFLPWLLDGEKDAGKFQEGIERMKIKTRQYAKSQVKWIKNTLIPDIKGSNMYILDATDLSKWHQLVAETAEPIVQKFLEDDKALEGVVQAPDHLQSLLESDFHKNSKNVDNPKTLNLGKKYVCEICHDKDQDKLIFIGDKSWSDHLNGRKHRTTIRKTEKRKNYEIWKLKQQNTEENETAKNISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R884</accession>
    <name>A0A1E5R884_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pheromone-regulated membrane protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3424</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R884">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R884"/>
    <dbReference type="InParanoid" id="A0A1E5R884"/>
    <dbReference type="OrthoDB" id="4035655at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ83104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="310" mass="34300" checksum="EC5A2DAB69905451" modified="2017-01-18" version="1">MNTSDMYIQDTYIEQEKKRESSKNQKKVLKNVTKGASVVMTILMLLVFVNLTHPISDFYDLTNSSTSKQSTLMDTTFSGSKHTTKDETTSSLAFSQTKILSNNRPVATDTRLIQPESSKANGGPVVKDSIGKGTKSNQKDPVDXKGSLYSSTSEDTKTKQTXKKLSDQSSVTLSPSDMSSIAAELHEILNTSPVVIFGKKNEPDSEYLYNLLTKEYEVTPEPIFVDLDKHASGSMIQKYIQTKKIVRHKEVDESNTMDIPYLFINGVSIINTGVATDIKKNHASNKLLDKLKALASDTVSFTRLESPSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R892</accession>
    <name>A0A1E5R892_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3477</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R892"/>
    <dbReference type="FunCoup" id="A0A1E5R892">
        <property type="interactions" value="567"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R892"/>
    <dbReference type="InParanoid" id="A0A1E5R892"/>
    <dbReference type="OrthoDB" id="2649at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050684">
        <property type="term" value="P:regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000770">
        <property type="entry name" value="SRSF protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000275">
        <property type="entry name" value="SRSF protein kinase 2 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051334">
        <property type="entry name" value="SRPK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634:SF9">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="221"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="30"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="384"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="428"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="56"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="392"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="436"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="446"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="470"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ83129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="746" mass="83352" checksum="8F9B0ED60D84AFD7" modified="2017-01-18" version="1">MGSSINFDLLKNSGKIQELENSGFMHKYQDVSEQQHEYQQQQQQHEYQQQQQQQHLPNHTSSQEASSVANSRSEQFQQTEPQNDIFSTSFANQNATTSNKSKLSLALQSGVSIASGSDMHSKNVGTVQENGIKLTVTTNPLSDDEYDEVDYADIAKPSNFENGIQIPNLGRHFEEHAPEKDDSSSDSDEELNTKYEESITEYKPGGYHPASKGELYKNGRYVLVRKLGWGHFSTVWLAKDLQNNFHVAMKIVRSNQVFTEAAVDEIKLLTAISSNMITNPQLEGSKHVLTLLDNFIHSGPNGDHVCMVFEVLGENLLALIKQYEHKGIPLVYVKQIAKQLLLCLDFLHRYCGVIHTDIKPENILMEIGDVEGMMRVIEMQNEEKKKLKNQRKNSFNSVTPGSSGSKFDPAATGNATAPLLPLSYGKPALKGIDHKSRTKKKRRKTLLASSQPLPSPLSSKEFSEMKNTFLTLGTSSSGESSDGKACGGQCRSSNGDISKSFETKLELTDDCNCEAPSIFNENEEDTIEPANSKSSSNEKNIINIKLADLGNGCWIDEHFTNSIQTREYRSPEAIIGYKWGCACDIWSVACLVFELLTGDLLFEPVAGKSYGKDDDHLAQIIELLGDIPPKMKYYGKHSTEFFDSSGQLMKISKLNMWPLHNVLIEKYKFSEHDAKEINDFLLPMLAINPKERADAGGMANHPWLKDTLGMENVTCCDRKLYGKGIDIPGWYCQCKKASGTQTDDNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R8A3</accession>
    <name>A0A1E5R8A3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3330</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R8A3"/>
    <dbReference type="FunCoup" id="A0A1E5R8A3">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R8A3"/>
    <dbReference type="InParanoid" id="A0A1E5R8A3"/>
    <dbReference type="OrthoDB" id="332863at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070290">
        <property type="term" value="F:N-acylphosphatidylethanolamine-specific phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070291">
        <property type="term" value="P:N-acylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070292">
        <property type="term" value="P:N-acylphosphatidylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024884">
        <property type="entry name" value="NAPE-PLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15032">
        <property type="entry name" value="N-ACYL-PHOSPHATIDYLETHANOLAMINE-HYDROLYZING PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15032:SF4">
        <property type="entry name" value="N-ACYL-PHOSPHATIDYLETHANOLAMINE-HYDROLYZING PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038896">
        <property type="entry name" value="NAPE-PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="17"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="3">
        <location>
            <begin position="194"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ83118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="469" mass="54639" checksum="21861F4B5451815C" modified="2017-01-18" version="1">MPANARSFRRLRAFGKYSFFGILVPYSGYALYITLNSNKEITKRQQLYKNGNDVADSKDSLFAKYTPLKILGRYENPFEEYRIQTLYEFFFNRFVELFETNRGGIPKTSARMEELMPVHKPTWVVQGQIQEDRIVHSEILDRSGSDNRGKSELDGNESGSKDDKKTIPSPCKVMDVTWLGQSCTYIIYENRFKVLLDPLFSDFLINEKFGPQRITRLPCAIEDVPVPDMILVSHNHPDHLDEKSLRHFNSVKSSTTKRNININKNKNKAALRKPPLWVVPHGMGKYMDSFQISNYQELSWWESLKLTTERGHSYFVHCTPTMHWSGRNLIDTNESLWCSYLVATKSNTNLFYHAGDTGYVHDLFRIIKENYGRTQLGILPCGQYCPQWHQRPRHISPEEVLRIMKDMQCTQVLGVHWGTFLLSGEYFREPKEKLETLAQWKGLDPQNCYCPELGKTTRFEIKQEADQVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA45</accession>
    <name>A0A1E5RA45_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative lipase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2545</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the putative lipase ROG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA45">
        <property type="interactions" value="153"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RA45"/>
    <dbReference type="OrthoDB" id="273452at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF24">
        <property type="entry name" value="LIPID DROPLET PHOSPHOLIPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="327"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="DUF676" evidence="5">
        <location>
            <begin position="28"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="DUF676" evidence="5">
        <location>
            <begin position="136"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="101"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ83764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="494" mass="56243" checksum="8DC301B30AE20893" modified="2017-01-18" version="1">MDINDENMEPETLHETVCSNVEEKTSGSAHLYVLLHGMWGSDKHMFTIRDQLLLQDPECTVFIPCKNGFFKTFDGIELIGDRVIDEIKLFLLENSATKKEKTKKEGTSDGFQNSSNSSNSSXXSSGSSXSENATTFTHISFISYSMGGVVARYCLGHMHDFLSKELNLQLQVFATFATPHMGINFYKPYFPYTFLTYLGSTLLGRSGRQLFLNDNKSKSDENRLLLKIARGKYLHALSKFKYKVTFANSIHDRTVAFYTAFITDKNFVCDEHTFETEFTNIENFPASSILDVTKAPVYISNDSLAESPTVFFFSKVVQVLTKIGKTLLFTLLFLFFFPIMLLINVTGTIYSYSRIXKVLKTLNSEDPEAQSLQEEFQDLESDWEVYGSDGYSQDGNDSVSKQFDHNMEAFISKYSQNEKNYAHDIQKLSFDHARKVIYHNLNSLKWIRVPLSLKAFNAHRAIVARSGMKKLAKQDADTLIFNMKLIAYLSERPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA69</accession>
    <name>A0A1E5RA69_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor eIF2B subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">eIF2B GDP-GTP exchange factor subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3127</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA69">
        <property type="interactions" value="1200"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA69"/>
    <dbReference type="InParanoid" id="A0A1E5RA69"/>
    <dbReference type="OrthoDB" id="10249309at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1070:FF:000002">
        <property type="entry name" value="Translation initiation factor eIF-2B subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1070">
        <property type="entry name" value="Translation initiation factor eIF-2B, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051501">
        <property type="entry name" value="eIF2B_alpha/beta/delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042528">
        <property type="entry name" value="elF-2B_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45860">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45860:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ83771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="309" mass="34406" checksum="908BD605883694CA" modified="2017-01-18" version="1">MSEXFSIKDTYLHFLEQDPEMTMPIAAIESLITLLQHTQPSTSAEMIQVIHKATAELTRSIPNSISLKAGCDIFSRFVLKNTHELSKKNDSKDWETFKQHLIENGQLFIDRAKQSREKIASIGADFIRDGDIILVHGYSRAVSHLLSKAAERLIRFRCIVTESRPTDGGLLLCKQLQEHGVVCNMIVDSGVGSIIHKVDKIMVGAEGVTESGGVINLIGTCSIAVLARNAKKPFYVVSESHKFVRMFPLSSNDLPSDKDPLKFSSKWDGQLSSPLLDFTSYDYITALITDLGVQTPSAVSEELMKMWYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAE4</accession>
    <name>A0A1E5RAE4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g2560</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAE4">
        <property type="interactions" value="287"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAE4"/>
    <dbReference type="InParanoid" id="A0A1E5RAE4"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000499">
        <property type="entry name" value="Serine/threonine-protein kinase KIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF10">
        <property type="entry name" value="FI20177P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="134"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="65"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="860"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1136"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1500"/>
            <end position="1543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="65"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="82"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="863"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1143"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1157"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1167"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1509"/>
            <end position="1543"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ83876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1543" mass="169254" checksum="B2C7AD058D91D9EE" modified="2017-01-18" version="1">MPNAFNNGGSGTPFGXVHNVDPALXKTRSANPSSNNTPLDNXSNHHSSGSTLNLARIHTSLARNASNASVQTAGSSQTPVLSSTTSASHLHSNSALSMNRKRSNSNTLLLQQSASHSKLHLPIQTPAQNVTKVFKRTEVIGRGKFGVVYKGYNIKTKQVFAIKVLNLDTPEDEVEDVQKEIQFLSSLKQIPNVTRYYGSYLNDTKLWVIMELCSGGSLRTLLKPGVIEEKYMGVILRELLIALSYIHSDKVIHRDIKAANVLITSEGHVKLCDFGVAAKLTQTRLKRQTMAGTPYWMAPEVISEGVSYDTKADIWSLGITAFEIATGNPPYCELDALKVMQLISKSQPPRLEGRQFSASLKEFIALCLDEDPVERLNADDLLKTKFIQNYKNIKVVILKDLLTRYQHYKEKNKAQIRESLMIDDIPSIKKENAAPLISSSNTVDVPATPLPLQPGEKKMYMEPLKKIDSNTNKDTGEDFKWDFDSLSSRDYVMPEETETNMPLGMHADEEHDWDEENVDDRNFNFQHPGTIHKDFTYFNTATQGRQYTNNTLNKSTIIPTMSHTNNTKTMLFNTPDHASMHGAHMHPTNSSTSNHNIYNNTFTQSNRAGAYNTNLNINTRQPSQHMVSTNNHNSHAVTTGSAYTRKFTANNYAEQPPKAPKHLMMLFDQEFNDHFGSVDAGVTPTTIKMNENFASDLHNRYDTNDHFGDYKRSDSLGFHSNSTLMHPAQKAKRPDIFSAAQHNQSVPVLPSLNTNLDMGSSSLRPYDSSSGLASAPLSHSHLNKENHIVEIEIPDELPVARTPSMAAIPMQIKPRSRSSTFSSNANTAHGNAHIQRRPTYSGAMHSAAGIARKMSPSKPELMVNTSTTSVPAPTNPVSLLENDGKRPGNGSPLSRESNRAMSKQHVVHISDTSDGPNVIMEKSAVGSNVGFTPSPKRNRNVSIINGQINGVHVXSQLSVSPSKQTDGVISLSYSTNXGNEMDKYNHTTSRIVTSPLSDMDGHGLIAADGTKFPLANKETDSNLHNMNTRLANNFKRNNPNLKLQMPSPTVANDAVFLGNSDTVSSATANXYNSAAAAAATAVAGDSGNNGSGAAGGTINQFGFNTNSTNNLPIAMTPLSEKPMIFNPITGVGSAATKGGSGGMKNNQMDGNFNSMEDXSKLNKIDTQENSQNPVSDPSSNRASNLSTPRDITMNHGSRNLGLNFPHGSRNAGTNNEIRDIDYTSATTASVPAPAGFVNNNGNIGSAVASPQAAFNNISNLHRSASLLHASTTDQNSGSIRLXHRANVTKPSALSFGNEQITEKDTVEGSGVVSGMVSDKDVTRHKAKSTAFPTALEAEHEENDTLMERLIEIAPLDENDFLSLRSMLLEYPAIKSEILDDDDDGELLNRSEQTHKSNLDLIMEEERDHYYYYSYEQEQKDMILTELNDAINQFENSLNVLDNEFKVMMSLKTQFGEDQDLLIYLEEDEKNGNCENEQHSYRSHFRDDKGAALKNSVSGIEKNTFKKDSQVNPTPNSAMFPSSENSPSEQPISIASDSSSKLHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAF2</accession>
    <name>A0A1E5RAF2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Vacuolar protein sorting-associated protein 71</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2623</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAF2"/>
    <dbReference type="FunCoup" id="A0A1E5RAF2">
        <property type="interactions" value="110"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAF2"/>
    <dbReference type="InParanoid" id="A0A1E5RAF2"/>
    <dbReference type="OrthoDB" id="74807at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="273" mass="30890" checksum="6EABEFB09E746943" modified="2017-01-18" version="1">MIEIEEIDRKSYNPNVYFSSADSFKVSKRAFNKSAGSSRLGKKKINYSLADMETQIYQRSQAENETGESEDGAGINKTNVAGNRGNAGGKLTNQASERQMLQSEKRFFELDTENASDFKYVPQLLSSLTGINKDKLMDSSVSLSQQRVSKRFEMPKNIKLLYKCTMPPTGLLRKSKIHSNRSENTNKSKATMIKKILSSKRSLSSYIEGLDRLNFKIIFKNVYNKRFFKVLRLMNICSNCGNSSNLSQCKKCHGKICNSVNCYILHSETRCDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAH2</accession>
    <name>A0A1E5RAH2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Zinc transporter YKE4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2774</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAH2">
        <property type="interactions" value="173"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAH2"/>
    <dbReference type="InParanoid" id="A0A1E5RAH2"/>
    <dbReference type="OrthoDB" id="200954at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16950">
        <property type="entry name" value="ZINC TRANSPORTER SLC39A7 HISTIDINE-RICH MEMBRANE PROTEIN KE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16950:SF16">
        <property type="entry name" value="ZINC TRANSPORTER ZIP13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="411"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ83905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="459" mass="51655" checksum="75F19E5F8A21A58B" modified="2017-01-18" version="1">MAHGIHNHQHHHHAKDTMEKTQSNLLQALDKYFYTSNSLHNSVLSVFLLLCVPPVLIKXLPRKIVQPIMTSNKTLLFSLGSVLGELFLHSLPSLYFQTITTELDEVTTSPVVAGIAIFIGYTVFYTLDKCLLLSINKWFNKNGDSLDLGHSHSHADXDSNMNYAHAHTHXQIEQDTNVETICLEDDVPLQVTTEKITIIDDKSDAGKSKARADSVFYLNLFSDMSHSFSNGILLSSSFKTSKNYGISTTMALLIHEIPHVMGDYALFMQKYNFSFGQCLQQHFVSSLSTLIGVAFNYLIFNENKKILKNHSQVSKIPSIILEPSYLQKFQNNVHSFVSWFLVKIGTTNKSTAQYPPMSTIDTYTLTSSVSNTAPYVFNILYSLDIFEKLPKNVQTKIISFTNTDLLKYDEILIMGLSCGSLFYMIFNNILPEITNPKGFKDLFLQTCAVSLGFAALFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAI5</accession>
    <name>A0A1E5RAI5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Histone deacetylase</fullName>
            <ecNumber evidence="2 9">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g2818</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the histone deacetylase family. HD Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="2 9"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAI5"/>
    <dbReference type="FunCoup" id="A0A1E5RAI5">
        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAI5"/>
    <dbReference type="InParanoid" id="A0A1E5RAI5"/>
    <dbReference type="OrthoDB" id="1918432at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034967">
        <property type="term" value="C:Set3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000007">
        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003084">
        <property type="entry name" value="HDAC_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF36">
        <property type="entry name" value="HISTONE DEACETYLASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037913">
        <property type="entry name" value="His_deacetylse_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01271">
        <property type="entry name" value="HISDACETLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="13">
        <location>
            <begin position="46"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR037913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ83881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="449" mass="51346" checksum="008D475C0AF7DB00" modified="2017-01-18" version="1">MTSTFEYDESTKPTYPLFENLNSTYKPRVSYHYNAGVSKYHYGVKHPMKPFRLMLTDHLVSSYGLHKIMDLYETREATKEEMESFHTKDYIEFLSKISPENSGRLSRATLEKFNVGDDCPIFHGLFKYASMYAGATLDASRKLNNQQSDIAINWSGGLHHAKKASPSGFCYVNDIVLAALDLLRYHPRLLYIDIDLHHGDGVQEAFYTTDRVYTASFHKYCGEYFFPGTGDVDEDGIDKGKHYALNVPLNDGIDDDSFICLFKAIIDPLINSYKPTVIILQCGADSLGHDRLGAFNLNIKAHGECAKFVKSFGLPLLVLGGGGYTPKNVSRLWTYETGIMNDVLLNEEIPHNIPFRDEFAPDLSLYPVLDDLYENKNSKRYLEDLRIRSLENIRYMQGAPSVRMDAEIIPTRDIRGLTEEEEEAIKEWNEEDERYKLLNDDVSRVGEFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAJ7</accession>
    <name>A0A1E5RAJ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Needs CLA4 to survive protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">UBA4</name>
        <name evidence="17" type="ORF">AWRI3579_g2710</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">In the N-terminal section; belongs to the HesA/MoeB/ThiF family. UBA4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAJ7"/>
    <dbReference type="FunCoup" id="A0A1E5RAJ7">
        <property type="interactions" value="857"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAJ7"/>
    <dbReference type="InParanoid" id="A0A1E5RAJ7"/>
    <dbReference type="OrthoDB" id="10261062at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070566">
        <property type="term" value="F:adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042292">
        <property type="term" value="F:URM1 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14686">
        <property type="entry name" value="bZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00757">
        <property type="entry name" value="ThiF_MoeB_HesA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000014">
        <property type="entry name" value="Adenylyltransferase and sulfurtransferase MOCS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000033">
        <property type="entry name" value="Adenylyltransferase and sulfurtransferase MOCS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03049">
        <property type="entry name" value="MOCS3_Uba4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028885">
        <property type="entry name" value="MOCS3/Uba4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF102">
        <property type="entry name" value="ADENYLYLTRANSFERASE AND SULFURTRANSFERASE MOCS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 17" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 13" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9 13" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="72"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="16">
        <location>
            <begin position="343"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="2"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate; for adenylyltransferase activity" evidence="13">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine persulfide intermediate; for sulfurtransferase activity" evidence="13">
        <location>
            <position position="401"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="107"/>
            <end position="111"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="168"/>
            <end position="169"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00075323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ83924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="444" mass="49452" checksum="015C36142309CC49" modified="2017-01-18" version="1">MITTTTEEIFQLKKQIEELKTENAQLKKTNAEVQNEKALPLSISEYSRYGRQMICDVTNGVKGQLKLKNSKVLFIGAGGLACPALAYLAGAGVGTIGIVDDDVVENSNLHRQILHSSAKVGMLKCESAREFLTSLNPHIKIVTHPVRLSNDNAFEIFAKYDIILDCTDTPITRYLVSDVAVNLGKMVVSASGVQTEGQLCILNFKNIGPCYRCFYPTPPPPHAVSSCSAGGVIGPCIGLVGVMMAIETMKIILDVYTIENFKPFLLQYNGFPEQSLRNFKMRGRQAKCQSCGDTKTIDRTAIESGKINYFAFCGSKNYNVVSPEERMTVHEFETKYWNNNKKPRDSYVLLDVRPTHHYEISHLANTHNIALKDLQNLKGDMTKLQSKIKSISETSEVVVMCRHGNESRTATRLLKDEFGVANTKDVIGGFFKYVDEINPQLPKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB03</accession>
    <name>A0A1E5RB03_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Importin beta-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2609</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the importin beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB03">
        <property type="interactions" value="252"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB03"/>
    <dbReference type="InParanoid" id="A0A1E5RB03"/>
    <dbReference type="OrthoDB" id="2016913at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051345">
        <property type="entry name" value="Importin_beta-like_NTR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363:SF33">
        <property type="entry name" value="IMPORTIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363">
        <property type="entry name" value="TRANSPORTIN 3 AND IMPORTIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="868"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="886"/>
            <end position="898"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ84077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1236" mass="140818" checksum="DBDB6FADE1761195" modified="2017-01-18" version="1">MSQSIGXIIQLIETLYSPTPPANINEIQQHLQSIQKNEKIGIELAKTLLRLDSKLSAQCKYFGALTYTVQINHYLSKFSNSENAILDDERPYSSNGSPPPDDDVPVFLKTLLIDNMSFLIQYSSFYIKGECEELFLVIRKLMSNLSVLFININKDLIDLIVPPGGSKNSAGWKNPINSLIYALLHIPDGNISLINDSVMLNIINETISYDKLIEFISFSKYSNKLLITFLSILVEEIMKTSSTRILPFQLTNVYHIIHDNVYITGLSIFMENLQQRIVCTTPGGGAAAASELHKMAYTDELLFESLTSWFQYSQTIPQVSGQSIDLTELFVKLMELMCNDNVDFKYSSEIVNIFYHLHDVNSLVVLNYEIRSRLEKLIFPTAPVTIDNSYLSSSSPLLRNSNWIMEYMNYLVTNELYENELKNLALLMPNVLSNSMTKTCNNFFLRENRGNQQIQEHVQLLLQYTNFPLVPITQENFSIQMLNFWCDLAEVFASLDDRSSEGNTLYDHEFAISLFQQCVEIYIPKLSLHNKEKVLEEANDTGETQLILEFDDFRKIGTEFIDQIWSILGHTHLTSKLMELSLNNNNDIYQLEVCSMLLQQLCEGTNFQQSPMLSDALEQEDSKIINQNIKMLEVGLTHPVLKNDFIKTATRFLATVASYFNISDNKKPVLNHCIDVLFKTLSDSAKNNQETEFMICKTLTTLCETCRFQLNSYLDSFMDILQTMLQPSDPSNAATNVTSNGIKISALTREKFSGSCGSIVTSFDKHAIKDTTNFPHLAELQKAYINKFLDIVSECIGKSPSCFTMEQDLSDYVLSLLKCIHSFNEGLLIFNDDEDFDGNGPHISTTLTSFSYSAPSTTXGNLNIDSASTTIDLSNDGENNGGNENGDGDGDDDDDDDYGSNGISSTNPMVFHYQQFMIQYWSIPENYSLLVQKLLHILNDLVFVNLASVSVNNSKIIEQANMIMSQYFSANLEQQPFPFKFEITEVMGYISNKFSACPFAKCFESNLTVLKKLVQYMNKLKMIGKDEFDYIFNNFVVKYYFVEGGILSDPDYIQTCIEFVNNILETSPRLIIQSAYFESFLVPEFLKLLXLVREKFTIIPLCKFWIKLLNNKKYDQDCVVYVNGLFNSPSPDCLGYRLVKNCFLIILNSNRSEVMQYSDVIRTLVSKHPLYCKKWLQMCLLEDQDLSQLLHRQTNTTSSGINHAKFIEKLMITSGSKRACIVINDWYLQCNGLPHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB97</accession>
    <name>A0A1E5RB97_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Prefoldin subunit 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2957</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RB97"/>
    <dbReference type="FunCoup" id="A0A1E5RB97">
        <property type="interactions" value="813"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB97"/>
    <dbReference type="InParanoid" id="A0A1E5RB97"/>
    <dbReference type="OrthoDB" id="3972598at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23161">
        <property type="entry name" value="Prefoldin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21431">
        <property type="entry name" value="PREFOLDIN SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21431:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="69"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ83843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="102" mass="11815" checksum="AAE72370548BE75D" modified="2017-01-18" version="1">MEKLEVLQTELQELVIARQKLETQFQENKIVQDEFKAIKGNNKPIYKLTGGVLLPVEYEEASVNVDKRLEFITTEIEKCEQNIKTKQTEMQKTKSEAIASKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBG6</accession>
    <name>A0A1E5RBG6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Malate dehydrogenase</fullName>
            <ecNumber evidence="3 12">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g2489</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="3 12"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBG6"/>
    <dbReference type="FunCoup" id="A0A1E5RBG6">
        <property type="interactions" value="859"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBG6"/>
    <dbReference type="InParanoid" id="A0A1E5RBG6"/>
    <dbReference type="OrthoDB" id="4069699at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01337">
        <property type="entry name" value="MDH_glyoxysomal_mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000013">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.110.10:FF:000001">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001252">
        <property type="entry name" value="Malate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010097">
        <property type="entry name" value="Malate_DH_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01772">
        <property type="entry name" value="MDH_euk_gproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540">
        <property type="entry name" value="MALATE AND LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540:SF73">
        <property type="entry name" value="MALATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00068">
        <property type="entry name" value="MDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 12" id="KW-0520">NAD</keyword>
    <keyword evidence="5 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="13">
        <location>
            <begin position="24"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="14">
        <location>
            <begin position="170"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="29"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="140"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ83913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="341" mass="36325" checksum="51DB1C88BDFA2F53" modified="2017-01-18" version="1">MFSTKALNKSTQRLFSTTNPTNFKVTVLGAGGGIGQPLSLLMKLNHKVTDLRLYDLRGAKGVATDLSHIPTNSTVQGFTPEESDGLKNALDNTDVVLIPAGVPRKPGMTRDDLFSINAGIVRDLAKAAAQYCPNAAICVISNPVNSTVPIVAEVFKKSGVYNPKKLFGVTTLDSIRASRFISEVEGTNPTQEKVTVVGGHSGVTILPLISQTKHSMMPKETREKLIHRIQFGGDEVVQAKNGAGSATLSMAQAGALFAGKILQGLDGEADVITPSFVDSPLFKNEGIEFFSSPITLGPEGAEKIHSLGNLSSEEEEMLATCKETLVKNIAKGKKFIDDSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBH4</accession>
    <name>A0A1E5RBH4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphoribosylglycinamide formyltransferase</fullName>
            <ecNumber evidence="2">2.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">5'-phosphoribosylglycinamide transformylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">GAR transformylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2627</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(1)-(5-phospho-beta-D-ribosyl)glycinamide + (6R)-10-formyltetrahydrofolate = N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + (6S)-5,6,7,8-tetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15053"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="2.1.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GART family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.2" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBH4">
        <property type="interactions" value="218"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBH4"/>
    <dbReference type="InParanoid" id="A0A1E5RBH4"/>
    <dbReference type="OrthoDB" id="5575075at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00126"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004644">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.170:FF:000009">
        <property type="entry name" value="Phosphoribosylglycinamide formyltransferase (Eurofung)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.170">
        <property type="entry name" value="Formyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01930">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004607">
        <property type="entry name" value="GART"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001555">
        <property type="entry name" value="GART_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00639">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43369">
        <property type="entry name" value="PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43369:SF2">
        <property type="entry name" value="PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00373">
        <property type="entry name" value="GART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184704" description="Phosphoribosylglycinamide formyltransferase" evidence="10">
        <location>
            <begin position="24"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Formyl transferase N-terminal" evidence="11">
        <location>
            <begin position="58"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ84250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="268" mass="29101" checksum="A0497E92832C7DC0" modified="2017-01-18" version="1">MAPPLKKFVLPTILAISSIVAIASLYQHKDSILPKKTSNDVSDESEKPLVSQTNQKPKKITVLISGSGSNLQAIINAIDSKTLXNVEINKVISSSKKAYGLTRASNNSIPTAVHSLYSYTKGLSKDAVAERKAARELFELELADLILQDKPDVVVCAGWLLILGPTFLSRLGNAVPIINLHPALPGAFDGTVHAIDMAYEKAKELQKPFTAGCMVHYVIEDVDKGRPIVVKELVIDSSKETLEQYEERLHAAEHIAIVEATNKVLSDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBI6</accession>
    <name>A0A1E5RBI6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Negative cofactor 2 complex subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2644</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBI6"/>
    <dbReference type="FunCoup" id="A0A1E5RBI6">
        <property type="interactions" value="874"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBI6"/>
    <dbReference type="InParanoid" id="A0A1E5RBI6"/>
    <dbReference type="OrthoDB" id="601405at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017054">
        <property type="term" value="C:negative cofactor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22905">
        <property type="entry name" value="HFD_Dr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000019">
        <property type="entry name" value="Negative cofactor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042225">
        <property type="entry name" value="Ncb2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46138">
        <property type="entry name" value="PROTEIN DR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46138:SF1">
        <property type="entry name" value="PROTEIN DR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="4">
        <location>
            <begin position="10"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="128"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="139"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ84256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="151" mass="17318" checksum="EEE56293832D0D07" modified="2017-01-18" version="1">MEANTGEDVSLPKATVQKIISEVLANTDITVSKEARDLIIDGGIEFIMILASMASDMADTENKKTITQEHVIKALEELEFQDFIPQLNDVINDHQKLLKQKQIRDSKLKKSGLSEEELIRQQEELFKKSRSRLNSQHQTPEELKKEQEQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBM8</accession>
    <name>A0A1E5RBM8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cell division control protein 53</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2907</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2 3">Belongs to the cullin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBM8"/>
    <dbReference type="FunCoup" id="A0A1E5RBM8">
        <property type="interactions" value="1085"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBM8"/>
    <dbReference type="InParanoid" id="A0A1E5RBM8"/>
    <dbReference type="OrthoDB" id="27073at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cullin family profile" evidence="4">
        <location>
            <begin position="455"/>
            <end position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ83983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="775" mass="89233" checksum="6EAE9C51DB8EDCD6" modified="2017-01-18" version="1">MAEVSAPSTMQNDMEPLETTWSFIESNLETILGPIDTKEATKALPSQLYMDIYTVIYNYCVNRSRFSQRQQQQLQQGESGAGGSTVLSGGDLYFKLKDYLKSYLVQLKPLDEDYLTFYCRAWKRYLVGAKTLHYAFDYMNRYWVQKERSDGRRDILNIYNLCLVCWKTYIFDNNSEQLVDELIKAIEYKRDLYAYSHNLQACPADAENRTESLIKTCVESLIKLGIDQHDLKKVNLSCYVSNFEDKFLEYTKEYYRKFSDKVLLQNDLNDDFVTILQDIYDFENASIVKLLDDHTKQPLNKALDEVLVWANKDVMYENLEKLLILDSNDNDDAATQRQVKLIATLYKLMKREASLVPKMASVFEHSVQTVGVANVRRLLKQNNMSITPKQYIKNLIATHAKFLQITEQCFGNDSMFIKALDYASRGFINSNDFATPSKMDAGENSSNINGDEISKTPEMLAKYSDLLLKKSAKNEQLVEDMKVSDIMIIFSFLNEKDEFETQYRRLFAKRLIHGTSISDAKEEEVIKNLQKENSMEYTGKITKMFQDIKLSKSLEIEFENAIQLDSEFSRNKYPKFEPFVLAETMWPYSYNKIDFKLPKELEPTYNGFEKLYSSKHSGRILKWLWPLSRGEMEAAIAKPGKPNFIFTLSLFQMSILLQFNTNLGESQSHQSNENTLQNLCEKTGLSQIDLLGHLIPFIKGKLITLHPGFGSATALHDLTVLRDPNVVLKLSTPYKALKTHINFANGVRPDIASNIMQSNDFNAQSSFKPESATDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBR1</accession>
    <name>A0A1E5RBR1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glycerol 2-dehydrogenase (NADP(+))</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2697</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBR1"/>
    <dbReference type="FunCoup" id="A0A1E5RBR1">
        <property type="interactions" value="476"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBR1"/>
    <dbReference type="InParanoid" id="A0A1E5RBR1"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000002">
        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="5">
        <location>
            <begin position="17"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="4">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ84321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="313" mass="34956" checksum="376F9D7B79B9ED18" modified="2017-01-18" version="1">MPSHEIKLNTGATIPQIGLGTWRSTDDEEAYNAVVSAVKDAGYLHIDTAAIYGNEVAVGKGIKKAIEFLGGRDKIFVTTKLWGTQQREPKKALQQSLERLGLDYVDLYLIHWPVALKTRNCPNGDLMSIPTNPENPGVRDVDLEDWDFTKTWALMQELPATGMTKAIGVSNFSVNNLKTLYKSPDFKIPPAVNQVECHPLLPQFELLDFCRENNIVLEAYSPLGSAGSPLLKEEVIIELGKKYNVEPAQVCISWGVNRGYVVLPKSVHAERIKSNKVIVEMSKEDIAKIDNIHKTQGIQRFVNPPFEPFPIFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBU3</accession>
    <name>A0A1E5RBU3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Deoxyribonuclease Tat-D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2976</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. TatD-type hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBU3">
        <property type="interactions" value="584"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RBU3"/>
    <dbReference type="OrthoDB" id="6079689at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008296">
        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01310">
        <property type="entry name" value="TatD_DNAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018228">
        <property type="entry name" value="DNase_TatD-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050891">
        <property type="entry name" value="TatD-type_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10060:SF15">
        <property type="entry name" value="DEOXYRIBONUCLEASE TATDN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10060">
        <property type="entry name" value="TATD FAMILY DEOXYRIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01091">
        <property type="entry name" value="TATD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="unsure residue" description="E or Q" evidence="5">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ84365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="415" mass="47820" checksum="6DB3047C7034064E" modified="2017-01-18" version="1">MXNPPIKQLVMNTIKQSNASTSYKYFDIGVNLIDPMYEGKYNGKQYHTCDIEAVLQRAERFGVQRMLLTGSSIHESLETIKLVQQYGTKYSFPLYYTVGVHPCCVNEFVTNGVSTIDKVXPVEYSEELLEKTKNLTRTKLKQLLDIWTQESFSTGFKSIGEIGLDYDRLHYSDWNLQKWFFEQQLKLACILNHNLTKSENNLSKSSNVSPPPLFLHMRNCCDEFVEILQKFVTGFQDSVDFYNYKEWVEKKQSHEKLNSFTYKFPENQVFVVHSYTDGPSHXSDLLNLSPNVYIGFNGCSLRTPESIATVEKVDLARILVETDAPWCEIKRSSASFEYVTETVASLDTTIVSVKKDKYTKKLQDMNNNANNIMVKGRNEPCNVNQVVEVISKIKKLSYDHVANTIWETSLRIYEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBV3</accession>
    <name>A0A1E5RBV3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein MMF1, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RutC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBV3"/>
    <dbReference type="STRING" id="56408.A0A1E5RBV3"/>
    <dbReference type="InParanoid" id="A0A1E5RBV3"/>
    <dbReference type="OrthoDB" id="309640at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019239">
        <property type="term" value="F:deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00448">
        <property type="entry name" value="YjgF_YER057c_UK114_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.40:FF:000001">
        <property type="entry name" value="L-PSP family endoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="entry name" value="RutC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006056">
        <property type="entry name" value="RidA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019897">
        <property type="entry name" value="RidA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00004">
        <property type="entry name" value="Rid family detoxifying hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803">
        <property type="entry name" value="2-IMINOBUTANOATE/2-IMINOPROPANOATE DEAMINASE RIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803:SF58">
        <property type="entry name" value="PROTEIN HMF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="YjgF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01094">
        <property type="entry name" value="UPF0076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="124" mass="13125" checksum="97931DAA3B6A2700" modified="2017-01-18" version="1">MIEAISTTNAPPAAAVYSSAIKANGFIYCSGQIPMTPDSKLVEGSIADKAHQVITNIKNVVEAAGSSLDKIVKVNVFLADIKYFAEFNEVYAQYFSAHRPARSCVAVAALPLGADLEMECIAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDJ9</accession>
    <name>A0A1E5RDJ9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2303</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RDJ9"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="436"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="453"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="477"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="495"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="542"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="581" mass="65807" checksum="3C7EE1E523060053" modified="2017-01-18" version="1">MPPAGPKRRRGGSSLRGRRSSSAHLASQQSHEELRKQRQREQLQANRLRCLSTGAGALSGIYKDQAETFPYKRRAAQNFSKDRRLMDVLTLHNVPNRRIQAISLKAHLPSIESLNSALKQKEEEIATLLSDQQSRGDENLSTFSDYSAITTSSVFEKEYEYLEKVESELHNSFLQLRNAFLENSKDSFKDTTLSIDSTSPEPDKNPTANLSDSKTELAAENSKTXTQNGRISTSDDKETIVEDDSTHTSAQNDPLMEKDDAQRKSPITQSKPVTTNINKLYEKTLQHYKLKSFCDIQVQNSEQMTFMQANNSMAPGNYWTDMYEKHLMHLKAKEEAERKEEEERKAKEEQEKENLRLAKERTEQEQILQKQFQQQRMAQLSNTSLLMNGQPSSLHDIXSPITASSAYSINDNNTAASXMPMGXNNNSNTNSVAVESHEVSAAVKEPNKPSLPNHMQTAPESSATSLLQELNDPSFFGGNTLSNSMMQSSSEANNLQQQQQQHQQQQQQGQQQQQQQQQELQGQQGQQELQGQQGQDQDQEQQDSHLDDKQANLEDPYFNDFETFDTEFGDNAFADFDPAFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDL4</accession>
    <name>A0A1E5RDL4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein kinase</fullName>
            <ecNumber evidence="8">2.7.11.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1824</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.21" evidence="8"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDL4">
        <property type="interactions" value="381"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDL4"/>
    <dbReference type="InParanoid" id="A0A1E5RDL4"/>
    <dbReference type="OrthoDB" id="408964at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13118">
        <property type="entry name" value="POLO_box_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13117">
        <property type="entry name" value="POLO_box_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14099">
        <property type="entry name" value="STKc_PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.30:FF:000005">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000091">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.30">
        <property type="entry name" value="POLO box domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033701">
        <property type="entry name" value="POLO_box_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033695">
        <property type="entry name" value="POLO_box_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000959">
        <property type="entry name" value="POLO_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036947">
        <property type="entry name" value="POLO_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00659">
        <property type="entry name" value="POLO_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82615">
        <property type="entry name" value="Polo-box domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50078">
        <property type="entry name" value="POLO_BOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="122"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="POLO box" evidence="11">
        <location>
            <begin position="597"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="domain" description="POLO box" evidence="11">
        <location>
            <begin position="698"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ84998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="793" mass="92087" checksum="6FF19D65AF9F911F" modified="2017-01-18" version="1">MPLSYPLQIVSDKHLNARRQYDDPAVGVSNNNNNNLMKTPVKYSSYHQRQIQIQHPSHDQQYSSNKENNTPIEYANQQAXNKRSDHHLESNKNQPAPKKKKEKLSALCKTPPSTIKMKGKDYKRGXFLGEGGFARCFQIKDETGTIYAAKTVAKSSIKSEKTRKKLLSEIQIHRSMKHTSVVEFIDCFEDDVNVYILLEMCCNGSLMDLMKKRKTLTEPEVKFITVQISGGVKYLHDRGVIHRDLKLGNIFFDQDYNLKIGDFGLAAIVSEESEKKYTVCGTPNYIAPEVLTGGKTTGHSFEVDIWSIGIMIYAMLFGKPPFQSKDVNKIYQRIKCGDFQFPPDKDETRYSRERVSEEAKILIEDLLATDPLERPSIDDIMDYVWFRKHFPPRLPYDVETASPHFDFSSLESFENFRTCMTKAGLLITEREKRKPIHIFKDGVFHTVRSDKYILPHCLSPSNAKAKYIELVDYQTHVKLNQFEREYRLQKAQQIKFEQKQREEEWKKKQLLAAQNSTSLLAESTNHIRSDLSLRILASECHMTLNGILSCEQQKIKYFEQKHQNERMSHNQYQNGVIPVSDDEXENISTIDSECPIIVTKWVDYSNKHGFSYQLSTDDIGVLFNNGCTILNLADADEFWYINADEKDGWAATHYALNTRPQEFNKHLEVVEFFSKYMQNNLNRVSTFKREVYHKDDVFLRRYTRYNQYVMFELSDGTFQFNFKDHNKYILSNNGHVMTYISPSREQFVFNLIDLMKYGSVPNYSGVDLQKVLAYIKEGLKQKSLILSQNQQVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDQ7</accession>
    <name>A0A1E5RDQ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GLC7-interacting protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2222</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDQ7">
        <property type="interactions" value="104"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDQ7"/>
    <dbReference type="InParanoid" id="A0A1E5RDQ7"/>
    <dbReference type="OrthoDB" id="3973067at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026241">
        <property type="entry name" value="GIP4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02082">
        <property type="entry name" value="GLC7IP4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="459"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="570"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="475"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="570"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="599"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="650"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="759" mass="86049" checksum="DF46A4C6453BC285" modified="2017-01-18" version="1">MLFRSAARAAASASTPSIQGGNIFDHLDAKNVQFQFSVDHLRRMLELLNAMHNSITSSENNQNIIPLTNLISSLCAGWFFNVHSTLKKRFALILNGKLIKLTSIKPILSTQLVEYKYDFLSPADSGEDFDFEKYKKKFYNFNHQLKSLESLISIIHNALQFYEVKLKQIIMERSSNRRPDASGVASVIDHESLVEFTHVQETQFSLDLAVLVEDKLKDTTDESFRNLQLQVLKKTQDVVKQFHDGTLCGFSKTMAALXNKETTVSQLPYWQFTFHRMYAFLLRCTQHSTFVAEIMHQLYYPSKAFFNDNRTKLFSKNLIEYEHMLALLEDTLDVVFLENMVKDFCENNVVIFFQTNTITSLYSRYIFEVEKCIKYRHAVLGLWIEHWEYLEKNIGDNLKEYTDFESSAQLLQLVEERRVNDRITCKKPLLENNVDIKAPSQPAEFKAAILRKPYKASNSTTKSVVRSGSTKKHSASSSTTSSRNRSNLASPQANSNPGSPRPGPVQAPFARAGRSRSSSLQHVKHTANKEAYNMKLAAAAAGLSTGMSGNDAAQKSHSLQLASLETQAEIQKQYSSQPKFSHQPLKPQPTSVLMKKSASENNRGSRQEISSTLQNELIPKLANVHLKEDTLRSSGKPMRTNSKKQEGKTKYTSSSESRQSDASIVVSDLDLKKTENSENEAYFDSEEYPHXIMVKKVRFTGVPPFNKKEDPPPTRRGWYIKPAVLHYPLIPCQNVGVQQNVSKQAEGLAFKRGHSLIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDT8</accession>
    <name>A0A1E5RDT8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1957</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDT8">
        <property type="interactions" value="284"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDT8"/>
    <dbReference type="InParanoid" id="A0A1E5RDT8"/>
    <dbReference type="OrthoDB" id="205099at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055122">
        <property type="entry name" value="Med14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013947">
        <property type="entry name" value="Mediator_Med14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12809">
        <property type="entry name" value="MEDIATOR COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12809:SF2">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08638">
        <property type="entry name" value="Med14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit MED14 N-terminal" evidence="10">
        <location>
            <begin position="63"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1111" mass="126745" checksum="61313EB2FEAF0E32" modified="2017-01-18" version="1">MTSVMNNNPASTDNANAIKPFQESTTGFKITQTNDPESGATLQEETPVRVVQPPPLPHVDVNQLPLSLLIRNLCIYSIGEIKQFFKLTKPNRRFEFLKLIIYLRNQFLRLYVLVKWCKTIKNNNFHLLIDMLNYLRQQNMNIGHCIWHLKNSVISSTINAKLPNPDLETALEVFNLGRPNLKNDIIIRNNNSGGSTKTFSSNNPISSFLNNNMIPAELILKKLRDLNVLLSIRVSMMELPAKFSFANHFIQDGRLIIRIPNEYEIHLSTATPQSPFYFVDFKILLMDVQTSDNTIYLKKYINDLLFREKDNSKVLHVLEHFLDKYLLSLKLYLCHKRLGRLSREKYSNNLXYHYDQKKSIINIKYWVFSRIVNPADCKITIGISKNEEAGDVELVLRWEIGSLQKLPFTIPTVYKNDILNNLFDAVDEIIFNHIQIIKHQLVSIDAGIFTDSKYDENNSDNNDGVRSFGDTESVGLNHSSSALLTGTANSTQENFIASSKNEFAKEEMSMSIPTKGAVSTSDSTSLTIYLPSTCTTTTPVKLKINPITGLLYFSNPNSKLLLSYMERLNKNVMKPADIVKSLYQLKLAKIVQIFTNMFTKCGWAVFNENVMKLSSETLHPHHKTWLKKNNNILIKDMFIRMSAWPANWYLVVSVLSSPSSCFVETKIAKIVSKQATWCLKYLSDNFKEKDDKEINSNMMKLEHVSYQRVDFLSKTILSKIINHIVLDSLNELGIEKNIVSESAKXKLPEYIQNLLSQDSNSAVIALNVNSLMKTNVSILDSTLFLLINCGIKNQQNKMSLFGRFTNVIDTQLLRSSDCTKLMIEFIDQQCFVMSTSDENNERIVDHLNSLGKQLAIFKKKLSHLIVLSDVITRLTQSFHSPDFQVVSLEPKEIAFNYFPKGDASDVSAADCTITLKDTDENSENSQLAKSQTKLEFELGATNPQMELNQFLRGANYEHEFLFKYFQFTSKFFFAIEQFKHTSTNQCPLFFNIHSMSXYEIAYLLFDEEISQPECLKISLEIRTKPVNRKEELLYYITVSNSKASSDSNSVIQKTISKISKACFEISKPATSKDGTQKLANCKSVIKLNSGVCCSDEDIEEVLNEIHNIVTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDV6</accession>
    <name>A0A1E5RDV6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NADPH-dependent diflavin oxidoreductase 1</fullName>
            <ecNumber evidence="9">1.18.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">NADPH-dependent FMN and FAD-containing oxidoreductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TAH18</name>
        <name evidence="12" type="ORF">AWRI3579_g2149</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">NADPH-dependent reductase which is a central component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Transfers electrons from NADPH via its FAD and FMN prosthetic groups to the [2Fe-2S] cluster of DRE2, another key component of the CIA machinery. In turn, this reduced cluster provides electrons for assembly of cytosolic iron-sulfur cluster proteins. Positively controls H(2)O(2)-induced cell death.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>2 oxidized [2Fe-2S]-[protein] + NADPH = 2 reduced [2Fe-2S]-[protein] + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67716"/>
            <dbReference type="Rhea" id="RHEA-COMP:17327"/>
            <dbReference type="Rhea" id="RHEA-COMP:17328"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with DRE2; as part of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Mitochondrion</location>
        </subcellularLocation>
        <text evidence="9">Relocalizes to mitochondria after H(2)O(2) exposure.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the NADPH-dependent diflavin oxidoreductase NDOR1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the N-terminal section; belongs to the flavodoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.18.1.-" evidence="9"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDV6">
        <property type="interactions" value="751"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDV6"/>
    <dbReference type="InParanoid" id="A0A1E5RDV6"/>
    <dbReference type="OrthoDB" id="1856718at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160246">
        <property type="term" value="F:NADPH-iron-sulfur [2Fe-2S] protein oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000030">
        <property type="entry name" value="NADPH-dependent diflavin oxidoreductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03178">
        <property type="entry name" value="NDOR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028879">
        <property type="entry name" value="NDOR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF10">
        <property type="entry name" value="NADPH-DEPENDENT DIFLAVIN OXIDOREDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0274">FAD</keyword>
    <keyword evidence="4 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 9" id="KW-0288">FMN</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 9" id="KW-0521">NADP</keyword>
    <keyword evidence="8 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="10">
        <location>
            <begin position="5"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="11">
        <location>
            <begin position="216"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="11"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="58"/>
            <end position="61"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="369"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="399"/>
            <end position="402"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="474"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="527"/>
            <end position="528"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="536"/>
            <end position="540"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="612"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ85079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="612" mass="71205" checksum="5C4C7301B8D2AA28" modified="2017-01-18" version="1">MGKKIAILYGSESGNAEEFAHSLSHKLHRYHFPHHVSSFMDYDPKDILSCKYLFIITSTRGQGDFPKNVANSDFWAFLKRADLPPTFLNHLNIAFLGLGDSSYSHFQYAVRKLQTRFVEQLGARELFPRYETDELGVKGSGGESVEAVYFAFEKQLISLLNEKFKTRKVDGKVVKRVPLDDSXYLXPSIVLQEAGTSHKSEQSTFHGVQKDLYTKAPIKXGKXLLNKRITAQDHFQDVRQLHIQCHDEYEPGDTLAIYAQNTEEDVESFLXAQPHWLSIADKTLVFENGLXPMNFLFDHQLTLRNLLKYHFDIMSIPRRTFFTKVWTFSTDSSRLSDGEEQLIQQREKLKEFGFDQDLQELYDYCNRPRRSVLEVIQDFESLRLPWEYAFDFLPPIVPRYYSISSSSNGSYAELTVAIVKYRTILHKLREGLSTKFIANLREEQDTVKYSIQRNFLLSRLSNQAHPVIMISPGTGLAPVKSXISSRIFSNHQYVFFGCRYKDKDYLYREELEQWNSNKELKLFTCFSRDISSSPNVKYVQDLLWENGAMVCQLIKDENAYIYLCGSSGKMPVQVKLTLLEILKKYGNFENDKSAEEYFQNLQKQYRYLQETW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE26</accession>
    <name>A0A1E5RE26_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Regulatory protein GAL4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g2039</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE26">
        <property type="interactions" value="866"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE26"/>
    <dbReference type="InParanoid" id="A0A1E5RE26"/>
    <dbReference type="OrthoDB" id="3364175at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000435">
        <property type="term" value="P:positive regulation of transcription from RNA polymerase II promoter by galactose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.240.10:FF:000009">
        <property type="entry name" value="C6 transcription factor (Gal4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051127">
        <property type="entry name" value="Fungal_SecMet_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005600">
        <property type="entry name" value="Gal4_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424:SF3">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03902">
        <property type="entry name" value="Gal4_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="12">
        <location>
            <begin position="21"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="112"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="697"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="824"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1009"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1081"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="112"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="146"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="697"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1009"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1098"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="13">
        <location>
            <position position="658"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="13">
        <location>
            <position position="816"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ85162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1112" mass="126106" checksum="C3E5A4CD83FCAAD6" modified="2017-01-18" version="1">MSVVKKESELIEISPELIEQACDLCRKRKLKCSKDLPQCKSCVKYGKKCVYSPKERRSPLTRSYLSAVEKRLFKLEKIMKRAFPNMSIEELADKVDQKDQLDIAESLISLQSAPSPSVAATPTRSAEQQSTFSADNFKAQTPTPSKHAELETSKNQDKKFAINEKDEHRNNKIFTVXATDPTLPNMSQESQMIDRINEQVSQGKKLVRDKKNDSGNQKIVPTDTPNDPLFGFDWTEVDENQKFVQNDGMAALTTDPNNKGYYGQGSSAILLRAALKNSGSLTQNKKKIEDLIVTTELEQQLRSLEIREMFINAYFEHYHTSYPFLDKEFFMMKFEEYEGADIDLQPTSADSDDTHTAERYLDWKILLNTVCAIGCWCLHGESLLNIDLFYYKEVKKCFKGTLFERGSILLVVSFTLLSNYAQKRNKPNSGWNYLGLAVRMAIGLGLYKEFDWHVTLGNSKYAHELEVRRRLWWGIYIFDAGVAITFGRPINLPNNFIYELALPSNIDDYSGHQFEGASESFQANKKKGGKSLPKPPQVVDYPTIYSGLIEQTKFTKISVEIHNRLLARPSPTAKECLQLNEKIKSFIGSLPSWFSKNDNTAFNSCSQLKKLSDNHEIPHWFHLTRYRLIWRYLNLQIILFRGFVWQNILGKNDANFKDFFENSTVNECIEICLDSSGQSIEYVAKYIESCKKPANANENTQFGSEPQQTTETANKNKNDVEQYSKLSILGSWYATYFLFQATLVPVICVVYHDARYKNFEIWKKQILVARRLLSVLQEKNKTAGDFIKIIDSVCGKILSGKSVSKNSKVGRPTLKDELLKTNDFKSSNTSSNKTKKVAKATGNGKKESSMKEKVLKKNLEKTGNAANINFNSPDIEKFFEETMPLDSNPWENTMDGNGTNLNSLLSLFNVPTPGMPNVNTPDYFKINNYEFGFSPKAESASQFESPKPGAYFDGNMNETSNPLFVPGLEKPQYANNFVNNKNEGTQMVGSEQAARSVLIPVPQLSSEIANDDQQPVPNNADLQSELLNPTTKNDSSELHKSISNSSFFPIWNEQSFYSTSINENAIKKDGEEIYRYIFNDTTASTPTPTPPVREHVSKGTQNTQPQGNFQNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE52</accession>
    <name>A0A1E5RE52_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AMP-activated protein kinase glycogen-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1939</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RE52"/>
    <dbReference type="OrthoDB" id="5873279at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="24897" checksum="1526C799884131D7" modified="2017-01-18" version="1">MXTNSIIYKSKAVENGISIAGTFNTWKPEPMLYNSSKKYYEYVLKKEELVGVVKVFFKFINNQTGDWFTDDNFSITQDEHGNNNNVMLITPEKKSNETRDKKMTLSNGLSPPAKSMTPGLEIHNSPAPTPKLNPSFDTPKDNNTNEVSNTKVDDKSALEIGVSDKNNSTIEIEVPGXDLNKNTEITKKNDQDIDYKNALQKLVLFIKNIFMSWVNWFKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE71</accession>
    <name>A0A1E5RE71_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Carrier protein YMC1, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2167</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RE71"/>
    <dbReference type="FunCoup" id="A0A1E5RE71">
        <property type="interactions" value="272"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE71"/>
    <dbReference type="InParanoid" id="A0A1E5RE71"/>
    <dbReference type="OrthoDB" id="409586at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990575">
        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF51">
        <property type="entry name" value="CARRIER PROTEIN YMC2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="21"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="118"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="212"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="300" mass="32275" checksum="A3FA77EAD2DA3348" modified="2017-01-18" version="1">MSDEIIAPQLIDETQTKTHESNVAMSLLAGTCGGIAQVLTGQPFDTTKVRLQTSTDPNTSAIKVVKDLIKNEGFKGFYKGTLTPLIGVGACVSVQFGVNEAMKSYFQKGDASKTLTLPQYFACGAAAGFANAPLASPIEHIRIRLQVEKSATANNGPLQVISKLRKHNALMRGLVPTMLRDGAGVGMYFAVYEGLVAKELNSHPGMRRSDIAAWKLCSFGGFSGCMLWMFVYPIDVAKSVFQTSNLATEKNLTTRRVLQNLYAKNGLKGLTRGFGPTLIRAAPANAATFLAFEATMRLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REI9</accession>
    <name>A0A1E5REI9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2446</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REI9"/>
    <dbReference type="InParanoid" id="A0A1E5REI9"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ85328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="184" mass="21708" checksum="BDB712814C3BEBAE" modified="2017-01-18" version="1">MTLIYFDVASIPTLIDNNDIFAFAIQKDAVEREYEKLNPVGSVMRKGEIAHFDPNANKIRVFLQFVRVVDEYDDLYENSEYDGIILEFKNYDGFRTQTLEPNRNENAIRKRAYFFMNRYLYKKYFISPKFSKIVQPGQLLDVWCIPQYVKRQSDLGDSLLYSISLISIIENPDDLKCLMELCKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REJ2</accession>
    <name>A0A1E5REJ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytosine deaminase</fullName>
            <ecNumber evidence="14">3.5.4.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Cytosine aminohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g2170</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the hydrolytic deamination of cytosine to uracil or 5-methylcytosine to thymine. Is involved in the pyrimidine salvage pathway, which allows the cell to utilize cytosine for pyrimidine nucleotide synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>cytosine + H2O + H(+) = uracil + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:20605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16040"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; uracil from cytosine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.1" evidence="14"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REJ2">
        <property type="interactions" value="146"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REJ2"/>
    <dbReference type="InParanoid" id="A0A1E5REJ2"/>
    <dbReference type="OrthoDB" id="408702at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004131">
        <property type="term" value="F:cytosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008835">
        <property type="term" value="F:diaminohydroxyphosphoribosylaminopyrimidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046087">
        <property type="term" value="P:cytidine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019858">
        <property type="term" value="P:cytosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008655">
        <property type="term" value="P:pyrimidine-containing compound salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01285">
        <property type="entry name" value="nucleoside_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000016">
        <property type="entry name" value="Cytosine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079:SF190">
        <property type="entry name" value="CYTOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079">
        <property type="entry name" value="CYTOSINE DEAMINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="17">
        <location>
            <begin position="8"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="18">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00066550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00074321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00084039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ85321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="158" mass="17562" checksum="97B31AF7C7D6B13D" modified="2017-01-18" version="1">MSVPXQQEIDKKGLSIAYEQALKSYKEGGIPIGGCLINNRTGEVYGRGHNQRMQKNSATLHGEIDTLENCGRLPAKVYAECTMYTTLSPCNMCTGSVLLYSIPRVVMGENKTFVGGEDLLKGRGVEVVNFDDAECYNLLQKFIKEKPHEWNEDIGVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REK8</accession>
    <name>A0A1E5REK8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nuclear fusion protein FUS2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REK8">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REK8"/>
    <dbReference type="InParanoid" id="A0A1E5REK8"/>
    <dbReference type="OrthoDB" id="10256089at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="1">
        <location>
            <begin position="122"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="624" mass="73821" checksum="62B6CD987408C59D" modified="2017-01-18" version="1">MQKTTTLVEHFQTSSKQEMTPIIEKHETKSFYENPYSNTLWATKSYNKEVLEDISTKSTVSRQKTLKRVLLNSVDQQIGAQHIESYIQDEPQRKKIDSPYRLKKDDFFTDTFETKKLACELSFIEVLQDFCNTETHYVKMLTLIKTVYFFELQKNQKLMTKIKSLAEMEGGXLILFGNIDTMIDLNKLLISDIKKRLSEIFNVNINSTEFWTTIKKSFIVQKLWNFEISKILQLAFXKFKQLYASYAISHKKQMFFLSFIEQHKTTKRLVEEXFSDCDCEYELEYLFKLPIERVLGWPVFLERLXENGKGVLHSDFLAALCDFQFEYNLYXKTFTENLNHHKEENDALKFARIXSNPSTNSKTSSLYSXHFSSNXSDKADMTLQEQKKKFMSIYQNMEKLKTMFQNMDLLFFVKSLYEIAAKWQTFVELLENEKXYSKFDTLNSNGNSMHGKYMKKLERQFLAVSRLQNNEISHNLVKNLNQMLGYCNKVQKIINXHYVLEKKQKNLTQKDAKYDAIXKQKLYLQTSLAENLPLFLQYCASFTNALTLRYYGIMLSYLEIMSGGAYFLQKETESLKNEKRELGDNFDILQLYTYNRFLTXNAVMDTWFEKDKSLYSKVFRRLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REL9</accession>
    <name>A0A1E5REL9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">High-osmolarity-induced transcription protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1872</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REL9"/>
    <dbReference type="FunCoup" id="A0A1E5REL9">
        <property type="interactions" value="274"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REL9"/>
    <dbReference type="InParanoid" id="A0A1E5REL9"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060963">
        <property type="term" value="P:positive regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052146">
        <property type="entry name" value="HOT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022210">
        <property type="entry name" value="TF_GCR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37784:SF2">
        <property type="entry name" value="HIGH-OSMOLARITY-INDUCED TRANSCRIPTION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37784">
        <property type="entry name" value="PROTEIN MSN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12550">
        <property type="entry name" value="GCR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription activator GCR1-like" evidence="2">
        <location>
            <begin position="1038"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="357"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="471"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="601"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="706"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="930"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="241"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="311"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="357"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="485"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="527"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="537"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="706"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="995"/>
            <end position="1004"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1139" mass="123782" checksum="EC2034E04A7349A7" modified="2017-01-18" version="1">MANNEANNHAHQQSLESQDNQPQDHQALEEPPTSNVSAVPEQLSLSMDKTQLQNRTDLSQPLPPQQASHLENSQSFPMSNPLLLNESYPHTGFTSQDQNANVMTTQPSGSSVPSFHQPTLHNPAMQHLTQETFGKQPMMHTPTTEKLIKFASQVAVDAEGNNVFGNPVVLDSRPSTPLQELNGTKNVGFDNAANTMNTKNANIKTSNLENLSLNNASMNNGNQLTNLKPFSSDGNALSLNMSHSPSTAHSSFSMTNHSHRPHPLQEVSSSMMNAAKGDPFGTNSNLSMDMPLVGSLDGKVTKNTYKESGLNNSTVSSHVSNNTENATQDTPLQVNNNGGGENNAINTINEAAQVNKYKSNNKSSSKSQKSFHGLSQAQLGNHSQNPSPSINQSIQANEQRNSEPHPLLITNYPDTQGIPVQATHNTTLSPHVVDTAMHESLGSSLNNVSFGVAANTGNLNVDGRRVENNRNIDPAQIGHNHRNSHGSSAAHSQKSSSATPLARRHSYVPNGTLLPNNTQTKRDSMHSHSSAQMASHPNSNLVSPTMSHSTHLHDAFTKDRFFGRSDGIDASVSHMAHPTRPTIDSNLSIDTKMLNAAANALSPNSAHGGHITHNQSPTSNSQSHSTTSPSSSLTTTKQILQRVSDVSKRLTYLEQKIGDLFAIQTQMMKDSQMYNQSVINRQTSFSTDGGASHSSQTNLMTHPINTMVSMNSHGPNNGLNNGVANQATDPSLLNTHNNASGLHMNPLGRQPFKLNANVLNRKRKAKQEKKKKHQLMQQQKLLEEQGLKEPQLVSNMLASASLPNMQVHNQNAPPSNNQSPVVNEAKLVQRKTVSPTTVSFPNAVNSIASVTGNDVIPSSSMPPSFGAVLGQQFLTPSYDIPLSTANMISSSRSPALTAFQAGIPKPQTSKTTDKNVATGNELRAEMTLETNNPHSLSHAPNIDNESSGITHNGHGKHADQSSNVGADHSASEERSGVSVNTSSGLVNEDGEALHLKTKRRRKKSTGVVSNNLGGKKRKINLDEFEKKNKESISKEIDYTMVKSPESVQIIWEEYIHGCNGNPSIMYLENTYGSKWRRQQNGKTFSRRKRIYKFIVNGMKEGKTAEDMIDLLEKKRIYADKDGKQKLRTIGWLQESLAGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RER6</accession>
    <name>A0A1E5RER6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cytochrome c oxidase assembly factor 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2114</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion intermembrane space</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome c oxidase subunit 6B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RER6">
        <property type="interactions" value="171"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RER6"/>
    <dbReference type="InParanoid" id="A0A1E5RER6"/>
    <dbReference type="OrthoDB" id="5545577at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.140:FF:000003">
        <property type="entry name" value="Cytochrome c oxidase assembly factor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.140">
        <property type="entry name" value="Cytochrome c oxidase, subunit VIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048281">
        <property type="entry name" value="COA6_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048280">
        <property type="entry name" value="COX6B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036549">
        <property type="entry name" value="CX6/COA6-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47677">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47677:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02297">
        <property type="entry name" value="COX6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47694">
        <property type="entry name" value="Cytochrome c oxidase subunit h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="108" mass="12781" checksum="D0454D58A9B9F47F" modified="2017-01-18" version="1">MVWNPLSSNNKKPIDDQSHAPSRSSRKLCWEKRDEYFACLDKNQILDSLDPAQQGKIKSXCKKEQNEFNYNCATSWIQYFQEKRLTEFKRTKMFEEMKANGAQLVKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF15</accession>
    <name>A0A1E5RF15_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Sterol O-acyltransferase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1720</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Sterol O-acyltransferase that catalyzes the formation of stery esters.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF15"/>
    <dbReference type="FunCoup" id="A0A1E5RF15">
        <property type="interactions" value="264"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF15"/>
    <dbReference type="InParanoid" id="A0A1E5RF15"/>
    <dbReference type="OrthoDB" id="10039049at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034737">
        <property type="term" value="F:ergosterol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008204">
        <property type="term" value="P:ergosterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014371">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408:SF23">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="338"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="377"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="525"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="565"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="651"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="705"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="37"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="150"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="63"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="179"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="194"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="663"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000439-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ85499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="725" mass="84352" checksum="11035A8A15B0F353" modified="2017-01-18" version="1">MGSTSANDTILQKKLVLHTKKLEREFALTRTKKINKSKFREMEKKTRDGINNAEGNGQDSKNNAEGENLNNNNLNNENNSEPMKKSLLNDVHFTNVGSILDVYVDGPIKKLFQGPLSDRRIEQITERNKQLYIKKVNTVQETTKTIEPEIIINNNNNNNNNSSSSNNNNKKKNKKNKKSNNDKDDKKNEHLDTETATTTTTKTTTTTTTETVAAFESTVQDIDPSVIDASVDVSDLLGQDMDKSESVHYKNGDSANNTNSEIIGGGVPANPKQLIHQEDLEDLYGEAEISSLTDFSGFFVLGWIACFFFVLKNLYVFYTQHGLHVMESEIWQYMSSGLFKVGAFDLVMYLSTYFTFAIQKLCYHKYLDWSTQGKHFVAIYEIVFLFGYLILAQFVVEFHWIAKIFIFLHSTVLLMKMHSYSFYNGYLWNLKQESDISKELLQFLKDHDELNKENSKILEKSVEYCESEIKSQSPKTNDPLTQQPLFPESLTVTNFFEFSMFPTLVYQITYPRNNKIRKVYVFEKLSAIFGIIFVMMTVAQIFMYPAALKAMSLESYPNSLEKWKLWIFACIDMIPGFICMYLLTWYLIWDAILNCIAELTRFADRNFYGDWWNSVSWNDFSKHWNIPVHQFLLRHVYHASMSFWKFNKVQAVLFTFILSSIFHELAMYAMFNKIRYYIFFFQMLQLPLTWFHYTLFVNNPVLGNCFFWFSICCGPTIVCCLYLTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF40</accession>
    <name>A0A1E5RF40_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Serine/threonine-protein kinase CAK1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2398</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RF40">
        <property type="interactions" value="274"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF40"/>
    <dbReference type="InParanoid" id="A0A1E5RF40"/>
    <dbReference type="OrthoDB" id="413582at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009891">
        <property type="term" value="P:positive regulation of biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF508">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="395" mass="45496" checksum="D718DC1964AF622F" modified="2017-01-18" version="1">MEVKWSQCQLVYTGLSELYRDQENPSTCYKKISKTSLPPPHNSQKELYFLKKLNTNGKANTHIVQLLNYIEDEVTDXVDPFYVFKFKYYQVSFDEYLLQYWKPKKPTLIANDERSTKPKRKKFTFSADGTMQLQAPVSEPSACNNHPIFVNTMTEKMAIDLFYQIVDGLEFIHANKIIHRDIKPQNILVAENGTLKIIDFDISYDVEHNNGPTEPDFTKKITDVSTSIYKAPELLFGVGNYDYGIDIWSLMVVLQHFALAEYMPAKDAQGTGQDIERLSRLKLPCKIDNGMEINYTKNFIVHGGSDIRLITSIFETFGIPSLEKWPTVSKYGSETFEYMFSAEGDPLYLEKIDFNSLFPVFSXNLVKECIAPMCVIEQTDRITSAELKKRLKMHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF99</accession>
    <name>A0A1E5RF99_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Mitochondrial import receptor subunit TOM20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2275</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Tom20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF99"/>
    <dbReference type="FunCoup" id="A0A1E5RF99">
        <property type="interactions" value="334"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF99"/>
    <dbReference type="InParanoid" id="A0A1E5RF99"/>
    <dbReference type="OrthoDB" id="2154253at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005742">
        <property type="term" value="C:mitochondrial outer membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016031">
        <property type="term" value="P:tRNA import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.10">
        <property type="entry name" value="Mitochondrial outer membrane translocase complex, subunit Tom20 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002056">
        <property type="entry name" value="MAS20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023392">
        <property type="entry name" value="Tom20_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12430">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12430:SF0">
        <property type="entry name" value="TRANSLOCASE OF OUTER MITOCHONDRIAL MEMBRANE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02064">
        <property type="entry name" value="MAS20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037707">
        <property type="entry name" value="MAS20_rcpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00351">
        <property type="entry name" value="OM20RECEPTOR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47157">
        <property type="entry name" value="Mitochondrial import receptor subunit Tom20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="166" mass="18383" checksum="1B13B9773A624176" modified="2017-01-18" version="1">MKTFYTTLSLLTTAALGYAVYFDYQRRNNVEFRRSLHKKALDFKKNQQARETEAAKEKLVEIQKYVITELAKDPLPAGAAAGGEDNSALFQKLVEDGEKLASQPGFAMEAAFSFYKALCIFPNPVNLLSIYQRSVPAPIYETIALMIAAMPPANVAPIISGQDNIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFB4</accession>
    <name>A0A1E5RFB4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">OPA3-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2007</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OPA3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RFB4"/>
    <dbReference type="OrthoDB" id="2129069at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019216">
        <property type="term" value="P:regulation of lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010754">
        <property type="entry name" value="OPA3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12499:SF0">
        <property type="entry name" value="OPTIC ATROPHY 3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12499">
        <property type="entry name" value="OPTIC ATROPHY 3 PROTEIN OPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07047">
        <property type="entry name" value="OPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="119"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="190"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="206" mass="23533" checksum="22365DD52D03EB6D" modified="2017-01-18" version="1">MSAIAIKLLAVALRQVSRPIANIIKIQAKEHEMFRNVCMRFAQRMHKVDATLRDKITPAKYRPLDPKTGKRKTIVRPLNDAKAVESGATILSEFFVFGVASSLVIFETVRQMKKETVRREQIASTLSFLENEIEKLREEVKIKDVEFLLLKEDLIKKGIESRKXLDLEFQKEKXSFVKNLLEGSGETADSKTPVNSQPVKQLNKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGA1</accession>
    <name>A0A1E5RGA1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">G1/S-specific cyclin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1746</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Essential for the control of the cell cycle at the G1/S (start) transition. Interacts with the CDC28 protein kinase to form MPF.</text>
    </comment>
    <comment type="function">
        <text evidence="6">G1/S-specific cyclin essential for the control of the cell cycle at the G1/S (start) transition.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGA1"/>
    <dbReference type="FunCoup" id="A0A1E5RGA1">
        <property type="interactions" value="356"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGA1"/>
    <dbReference type="InParanoid" id="A0A1E5RGA1"/>
    <dbReference type="OrthoDB" id="3969999at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007089">
        <property type="term" value="P:traversing start control point of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20559">
        <property type="entry name" value="CYCLIN_ScCLN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000080">
        <property type="entry name" value="G1/S-specific cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014399">
        <property type="entry name" value="Cyclin_CLN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21615">
        <property type="entry name" value="CYCLIN N-TERMINAL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21615:SF2">
        <property type="entry name" value="CYCLIN N-TERMINAL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001770">
        <property type="entry name" value="Cyclin_CLN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 6" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 6 7" id="KW-0195">Cyclin</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="9">
        <location>
            <begin position="74"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="372"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="417"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="469"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="417"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="490"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00053308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="542" mass="61050" checksum="59B579F6A7000B7A" modified="2017-01-18" version="1">MESVGLVITAKQHHYPIELSNLELLAHYETIQDYHQELSNNIVMKNFKFKPDLKLILQQPEMCPLDSFRFQIVNFLFQLSHKTRVSQGIFQASCRLYDRYCSKRIVLQDQAKLVISTCLWMTAKTYGGCNHIINNISVPTGGRFYGPNPRARIPRLNELIHYCGGYDKYDESMFVQMEKHILDTLQWDIMEPLLCDYILNVDENCLIQYELYQRQLASSKTLQMENNKHNSNNSTDTESENEEDEDLQDKIQLIHAKNFLIDLSCWQLELLQFEYFEIAHGIFKLINKVLPPSPQITAHPHTSILLTPQASVQQTNKVVNIFLDAIVNKKLPSPLMQQYSKNYKVMEFVALCSTKLGPSLFASNNDAVINNNNNNNNENMGSSSTANVYGTQTPQHSMHSNNSTAEMNHIISTAYATPPSSRTASTSVANTSSSSILSQGTTSTNSSQGNTPYMIQGKAFISGMNQTHNLHQQQPSPITPGIGGPMMHPLQNNTKFCFNNDSANSGTKMNRNENVSCKNSNSTQAKNNTTSTMGIGLIHQRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGL9</accession>
    <name>A0A1E5RGL9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Nucleoside diphosphate kinase</fullName>
            <ecNumber evidence="3 11">2.7.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1363</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the NDK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="3 11"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGL9"/>
    <dbReference type="FunCoup" id="A0A1E5RGL9">
        <property type="interactions" value="879"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGL9"/>
    <dbReference type="InParanoid" id="A0A1E5RGL9"/>
    <dbReference type="OrthoDB" id="2162449at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04413">
        <property type="entry name" value="NDPk_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.141:FF:000002">
        <property type="entry name" value="Nucleoside diphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00451">
        <property type="entry name" value="NDP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023005">
        <property type="entry name" value="Nucleoside_diP_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001908">
        <property type="entry name" value="PRK00668.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11349">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00469">
        <property type="entry name" value="NDPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside diphosphate kinase-like" evidence="12">
        <location>
            <begin position="6"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="9">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ86052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="154" mass="17128" checksum="8BFAAF369E3A2621" modified="2017-01-18" version="1">MSQGNTERTFIAIKPDGVARGLISQILGRFETKGYKLVGIKLVKPTEDLLNQHYAEHVGKPFFPKMLKYMLSGPILATCWEGKDVVKQGRAILGATNPLNSAPGTIRGDFAIDMGRNVCHGSDSVESAQREIKLWFKDEELVDYPLSGLSWIYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGM2</accession>
    <name>A0A1E5RGM2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Inositol phosphosphingolipids phospholipase C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g1592</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the neutral sphingomyelinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGM2"/>
    <dbReference type="FunCoup" id="A0A1E5RGM2">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGM2"/>
    <dbReference type="InParanoid" id="A0A1E5RGM2"/>
    <dbReference type="OrthoDB" id="387657at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004767">
        <property type="term" value="F:sphingomyelin phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000073">
        <property type="entry name" value="Inositol phosphosphingolipid phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038772">
        <property type="entry name" value="Sph/SMPD2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320:SF24">
        <property type="entry name" value="PHOSPHODIESTERASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320">
        <property type="entry name" value="SPHINGOMYELINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="441"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="467"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="15">
        <location>
            <begin position="74"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ86058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="526" mass="59615" checksum="7F62F5317D477DF3" modified="2017-01-18" version="1">MRKTTSYGSHNAEMSSDDNNSNDYLVVDGTQVAAQISNPANEDEVGYTTSIAQKIKSHTQEQGADNTPTTLKLLTFNTWGLKYVSKFRKERLRAIADKLAGLDTGVPLKEEQTFTGSSFDSQNETLDYDVVALQEIWCQEDWDYLVMRTKQIYPFQRWFSSGILTGPGLAILSKIPIEETFLYRFPVNGRPSAFWRGDWYVGKSLSVTILQSTSKDSKICLMNSHMHAPYAAQGDAAYYCHRSCQSWDISKMIKLYREAGYYVILVGDLNSKPGTLPYRFMTTEGGLTDSWELYHTRADGSSTSHSLQEIETMKTIDQIKYGGCTCDSTLNTWRASRLPTEACRLDYALVDGLKFQALSCGVAFTERIPSIGSYSDHFAYTVTLQLVSPDQPLQMGPMTPKFKDEELTHMLSLNRLKNYDDMLYVLNDYFETAYWQKRWRITHFFASVVFLVIMVIITTFTSNRAGWSSVLILLASCVVAITGLTNGFIGFLFGQKEIRALKEVEQEVRDSKQGLQLRMHMDKVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGY5</accession>
    <name>A0A1E5RGY5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein ASI3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1486</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGY5">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGY5"/>
    <dbReference type="InParanoid" id="A0A1E5RGY5"/>
    <dbReference type="OrthoDB" id="66726at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16616">
        <property type="entry name" value="mRING-HC-C4C4_Asi1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22696">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22696:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="367"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="435"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="435"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="813" mass="93113" checksum="D3F84F7EAAFABF0E" modified="2017-01-18" version="1">MSGNNSQASAGXASTSSGPSNGTALSMFQYLNKVKSLYEIGSELRKKEILNNNRLDXEGSSIXKARLKFMWESLQLLLKTPIYMVKSMHTAIQMDREALQLKDLMPKNSTSLSLSSSSSSSSSEMSTSSFSTTNNDNIVLFQFEMNYFFSFFSLSCIMSALLLARINYMSTTTLRRRRVNPTEQLALRKMEKISRTICYTAVILLLCVMLVFLYKQVQISQIADPLLINLKVFKIKQQQFLKLCFFAFVISEANSLLINNIYTIKKPSLTTSDYTLFDLSLLFYSFDQYPITNTKSLPFAYTVVIAYCIVSHLSINVLELIKKRKLWFKVSTTLNVGYTGWLLYLLYTGEYFALPMVVKYTSLPKLTSLFIIALSSFSFLLTCIVMGFKNLEILQYFKFFKNLKNHLNFTGEEEFNTIILKLSLFLTNSTSNNSNGNGNVDTSVNPFNTDTTSNTNSKDNLAQSNPLLSNDLGDVNMFKNIGMLNKFEAYPEHMFKDEEEETSQNANGLGNNSNGVWGNKMLHVHRFSPLYKFFYKLHVILLLPWKKVKKIDKRTEEVNGLTSLEKEIGYVNINEMINENNYYRLLINPKIGLFPDTDTSKDYECDSETSGQDDLELVEDILEENDEIDNLDENSDLDLVEMREELPELLHLDELIYEKNQCEQELTEVENLIEVEPTKDDSDFYYEVLKLSLASKKFAEKFTGKLTRSKYLALNEPDVLRSVVQEKRLEHWNRKQGEKVIFSHDDDDLSDLEDANSEHTCVVCKTNLRCVILWPCRCLCMCDDCRVSLGVRGFNKCPCCRSTVEGFSKINNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH02</accession>
    <name>A0A1E5RH02_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar-sorting protein SNF7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Vacuolar protein-sorting-associated protein 32</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1483</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH02">
        <property type="interactions" value="682"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH02"/>
    <dbReference type="InParanoid" id="A0A1E5RH02"/>
    <dbReference type="OrthoDB" id="5592979at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1060">
        <property type="entry name" value="ESAT-6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF10">
        <property type="entry name" value="GH13992P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="207"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="222"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="233"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="244" mass="26964" checksum="F6F9B64301985799" modified="2017-01-18" version="1">MWGYLFGSNSSKTNQETPKKAILELREHIQLLNKKQSFLLTQADTQKAQAKVYLQQNKKELAKNYLKKSKLLESQIVKISNQVDSLEQQLFSIESANLNLETMKAMKSGAKAMKHIHKGLDIDKVDDTMDEIREQVELGDEISDAISRPLNSGMANAVDEDELEDELAALMEEDAGQELGVQAKPQPAVAAVKVEMPSAPANKLATELGTSNKAAAAAAXXETEEEEEDEDERALRELQAEMGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH47</accession>
    <name>A0A1E5RH47_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glutamine--fructose-6-phosphate transaminase (isomerizing)</fullName>
            <ecNumber evidence="4">2.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Hexosephosphate aminotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g1438</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in amino sugar synthesis (formation of chitin, supplies the amino sugars of asparagine-linked oligosaccharides of glycoproteins).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH47">
        <property type="interactions" value="1100"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH47"/>
    <dbReference type="InParanoid" id="A0A1E5RH47"/>
    <dbReference type="OrthoDB" id="15235at2759"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00528"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00714">
        <property type="entry name" value="GFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000001">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000052">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047084">
        <property type="entry name" value="GFAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001484">
        <property type="entry name" value="PRK00331.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937">
        <property type="entry name" value="GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE, ISOMERIZING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="GLUTAMINE--FRUCTOSE-6-PHOSPHATE TRANSAMINASE (ISOMERIZING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 14" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="12">
        <location>
            <begin position="2"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="13">
        <location>
            <begin position="397"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="13">
        <location>
            <begin position="569"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="230"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ86186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="725" mass="80753" checksum="359D7AD96D87A14B" modified="2017-01-18" version="1">MCGIFGYCNYMVEKTRGEVMDTLVEGLSRLEYRGYDSTGIAVDGDEAEETLIIKQIGKVSALKEEIKRVNPDRSTPLVNHAGIAHTRWATHGPPSQINCHPQRSDPHNEFVIVHNGIITNFRELKTLLLNKGHXFESDTDTECIAKLFKHLYDTNLKMGFELDFNELTKQVLSELEGSYGLLCRSSHYPGEVIATRNGSPLLIGVKSKKSLKVDFVDVEFPGNDAKPESVLKSASSSSSQIHGSSSSLSLNQRAAAIAEDRILFSPLVNNETALRYSQSRAFLSEDGIPMPIEFFISSDASSVVKHTKKVLFLEDNDIAHIYDGELHIHRLKKQQDGETTTRPIQTLEMELAQIMKGPYNHFMQKEIFEQPESTFNTMRGRLDFENNTVRLGGLQNWLPTIRRARRLIMVACGTSYHSCLATRAMFEELSEIPVCIELASDFLDRKTPIFRDDVCIFVSQSGETADTMLALKYCLDRGALTIGIVNTVGSSISRSTHCGVHINAGPEIGVASTKAYTSQYITLVMVALSLSDDRVSVVDRRTEIIQGLRKIPNQIKKVLQMENQIKALCDKDLKDQKSLLLLGRGYQYAAALEGALKIKEISYMHSEGVLAGELKHGVLALVDEDLPIIAFGTRDSLFPKVVSSIEQVTARKGKPIIICNENDEVWANKKKDIQGLVLLEVPQTVDCLQGLLNIVPLQLMSYWLAVNKGYDVDFPRNLAKSVTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHB1</accession>
    <name>A0A1E5RHB1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphatidylinositol N-acetylglucosaminyltransferase subunit GPI1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHB1">
        <property type="interactions" value="87"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHB1"/>
    <dbReference type="InParanoid" id="A0A1E5RHB1"/>
    <dbReference type="OrthoDB" id="70250at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007720">
        <property type="entry name" value="PigQ/GPI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21329:SF3">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21329">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT Q-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05024">
        <property type="entry name" value="Gpi1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="358"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="399"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="435"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="472"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="614" mass="72085" checksum="35A602C24257170D" modified="2017-01-18" version="1">MAVIGQKLKGTENKFFVLSLVDEECKKNTLNSIPLARNANNTDRYCILGFFRQLDKNFKGCFEQGTKVQFVYFDSPRMWQMQFYSLRPINLSTPISLLLLQYSQENNYDAVELGYGCSNYTKFQEIYXKCHTSKYDNIQLVLDEVNIIDHHFNVLAEQFPGLSLQKRHQFFWKFSRRTRDMWHMCLTLLIVLIRIICEVWFQICDFFLLDTYSATGLQINLRCRQLCYFPLQYVNIWRKKAFKRQLTLSKSRYYSYLDYIKFYNTIWLIVNDVCFGFTLSSFLLINANTISTQVSKYLEYFFVDSVKSLTLFLSRSPFGIKLNDELSNFLKELFLGIIDFFNDLYLKKIVSPTLLSKVIKLVGLASYLCGATFALSICIDYLSLISLHLKLFYRISSKLYGWQLTLMRSLFYLFCGRKKNVLRNRVDNEEFTLDVMLMGILFFVILVFLLPTVFAFYITYSVLEYFTVITEIFFESAVMLLNHFPLFVLMLRCKDSKRIPGGIKFDICREDVLIFNNGITKKCVPGVKMETIPLNLAQIFGXFRQVMKDLSDQYFSLNTLKSIFLGQDITVRRKDLYATLYSSIPAKVIGIQDLRDLMEQSFNDSDRIINKKPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHE4</accession>
    <name>A0A1E5RHE4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alcohol dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1460</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10736"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHE4"/>
    <dbReference type="FunCoup" id="A0A1E5RHE4">
        <property type="interactions" value="1046"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHE4"/>
    <dbReference type="InParanoid" id="A0A1E5RHE4"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000947">
        <property type="term" value="P:amino acid catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08297">
        <property type="entry name" value="CAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000039">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000002">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF3">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="10">
        <location>
            <begin position="41"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ86320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="374" mass="40136" checksum="4A6E7315599233D6" modified="2017-01-18" version="1">MLKTFINTSKISTNRLVLRRLQSTYTIPTIQKGVTFYKNNGPLKYEDIAVPKPKPNELLINVKYSGVCHTDLHAWKGDWPLPVKFDPYLVGGHEGAGVVVAMGSEVKNWSIGDHAGIKWLQSSCMTCEACENSNESNCPDAVLSGYTHNGSFQQYATADAVQAARIPQETDLAEVAPILCAGVTVYKALKSANLKAGQWVAISGAAGGLGSLAVQYAKAMGYRVLGIDGGEGKQELFEKCGGEIYIDFTKEKNIVEAIKEATHGGAHGVINVSVSEQAISASTEYVRNNGTVVLVGLPAGAVCKSDVFSHVVKSISIVGSYVGNRADTREAIDFFSRGLVKSPIKIIGLSELPRAFMEMEQGKVLGRYVVDTAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHM7</accession>
    <name>A0A1E5RHM7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ribose-phosphate pyrophosphokinase 1</fullName>
            <ecNumber evidence="5">2.7.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Phosphoribosyl pyrophosphate synthase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AWRI3579_g1248</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">5-phosphoribose 1-diphosphate synthase involved in nucleotide, histidine, and tryptophan biosynthesis. Active in heteromultimeric complexes with other 5-phosphoribose 1-diphosphate synthases (PRS2, PRS3, PRS4 and PRS5).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHM7"/>
    <dbReference type="FunCoup" id="A0A1E5RHM7">
        <property type="interactions" value="402"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHM7"/>
    <dbReference type="InParanoid" id="A0A1E5RHM7"/>
    <dbReference type="OrthoDB" id="413572at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009156">
        <property type="term" value="P:ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000017">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000043">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000051">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000842">
        <property type="entry name" value="PRib_PP_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF57">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00114">
        <property type="entry name" value="PRPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="19">
        <location>
            <begin position="5"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="257"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="259"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="288"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00077829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OEJ86401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="423" mass="46666" checksum="140B54317F7FA756" modified="2017-01-18" version="1">MRKCKVFVGNSHPELGNLVCERLGIEPAPCTLKKFANGETSVQIGVSVRDEDVYVIQSGSQHVNDHIMELLILVSALRGGSAKKITAVIPQFPYSKQSKMKKHRGAITARMLANLLIMAGADHVVSMDLHASQMQGFFSKPVDNLYGGPSLAKWIKENIEDYENAVVVSKNPGGTKRVTALADALKINFAMIHTDRRRSKDIYAQNKGNMTQLKLRKQSMLRKNKPIIKQGENGEDEENIILTNGIQTARLVNGHVVNDEDPFDSDSETEEEDSANNSGSNTSREYDNESDDEDDDGTPDETEKLITLVGNVNDRTAIILDDMIDRPGSFISAAEHCVKNCGAKNVYVIATHGVFTGDCLEQLEQSDSIAKIVVTNTYPISEERLAKSKKLVLIDVSPIFAECIRRDHYGESISVLFDSLKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHN9</accession>
    <name>A0A1E5RHN9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1397</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHN9"/>
    <dbReference type="InParanoid" id="A0A1E5RHN9"/>
    <dbReference type="OrthoDB" id="69177at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF236">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="139"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="244" mass="28295" checksum="4ED72A4AC0168311" modified="2017-01-18" version="1">MPKKTKQEASKKVNCTKPTTAEYTFPNELHSLRKGNPSFKFLPQPVVEFEDKIILIRKFFSPSLCQALTKSFGNSAENDYQHMEPFVQRGSKDYAARFNDRFQILDEIVANEVLWPYLREILEQHEYVMKNLDFKNAQGLNPQLRCYRYTKGHYFGKHYDESVNISNGTNTTRWTLLIYLTGGPDELVGGDTIFHSSLQKNKTLSVHPERGMALLHKHGDDCLLHEAQMVKKGVKWVLRSDVYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHQ2</accession>
    <name>A0A1E5RHQ2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein MIF2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1395</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CENP-C/MIF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHQ2"/>
    <dbReference type="FunCoup" id="A0A1E5RHQ2">
        <property type="interactions" value="120"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHQ2"/>
    <dbReference type="InParanoid" id="A0A1E5RHQ2"/>
    <dbReference type="OrthoDB" id="1939643at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019237">
        <property type="term" value="F:centromeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051382">
        <property type="term" value="P:kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051455">
        <property type="term" value="P:spindle attachment to meiosis I kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028386">
        <property type="entry name" value="CENP-C/Mif2/cnp3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025974">
        <property type="entry name" value="Mif2/CENP-C_cupin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028929">
        <property type="entry name" value="Mif2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16684">
        <property type="entry name" value="CENTROMERE PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16684:SF11">
        <property type="entry name" value="CENTROMERE PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11699">
        <property type="entry name" value="CENP-C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15624">
        <property type="entry name" value="Mif2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mif2 N-terminal" evidence="7">
        <location>
            <begin position="3"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Mif2/CENP-C cupin" evidence="6">
        <location>
            <begin position="552"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="115"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="280"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="457"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="692"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="144"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="175"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="280"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="341"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="366"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="394"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="458"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="478"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="694"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF15624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="727" mass="83124" checksum="9A54671F812B84A3" modified="2017-01-18" version="1">MDYVNLGVRSRKTGIKLKPNLPRDEFSMENVSDFFADDTSELSTQQQQQQQQQYVQNFSNATNMQQQFYPQQFYLQKFFTNDQQHQQLQRNQNVHAMPFSSRALNSEQISSSLYVSEGRRRQSNYKKKRSSTLSLPPFDLSVDGDTSTQHVLPSTSDILQQQKKRSFTKRDRTFHNNTQTQLASNRTFNDDSVEPLDQPFPENSNSPNYKDSEVQIEPFQAGYGVSNDQQNSVQLTPIQGREPSEGHPSFEVYKSNLSGFINDSIRDEPTNGLVDIEAIVDDEEDDEVGSVNEDPELDDDYNDDQDNDVEEVTDLDDEEDVNYTDQRSRLHNVSLEPIQENSEENESSESDTEALFDGTKDLINNMDLTRGTSFEQDTQNGNMSSDDEYIASQAVANDSPNFQSSGLRRSSRIKVPTLDYWRNEKIVYKRETDKPGLSIEAIVRKDPAEEVDEEEILRKKKEEKRKQKNSNYSYNPSGKRRGRPRKKKEINSDILEKLDRGEVKTGEWIKFGILETSVKTATGLSKEEIVAFAPDTAQKEDTEENEDNMYSLSIIFDTYKEHFQTGMYKLPPEVGKKKETSSESFVFNCFVVQGILEVCLNSKSFICTQGSTFQVPANNKYSFENKGEDEVRLYFVQIAMPGMTSEVHNNSEQQMFLQDSSNYNNSNPSLKNNKSYTINETDEPQLFLNEDSFRQNSGNTGTMRKQASHSLSSSIGQPSSFSDMVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHR1</accession>
    <name>A0A1E5RHR1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Vacuolar morphogenesis protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1251</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the VAM6/VPS39 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHR1">
        <property type="interactions" value="672"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHR1"/>
    <dbReference type="InParanoid" id="A0A1E5RHR1"/>
    <dbReference type="OrthoDB" id="5325112at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032914">
        <property type="entry name" value="Vam6/VPS39/TRAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019452">
        <property type="entry name" value="VPS39/TGF_beta_rcpt-assoc_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12894">
        <property type="entry name" value="CNH DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12894:SF49">
        <property type="entry name" value="VAM6_VPS39-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10366">
        <property type="entry name" value="Vps39_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vacuolar sorting protein 39/Transforming growth factor beta receptor-associated" evidence="5">
        <location>
            <begin position="593"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="254"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="6">
        <location>
            <position position="529"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="6">
        <location>
            <position position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ86442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1030" mass="119731" checksum="D898D464230B8CBF" modified="2017-01-18" version="1">MSGEMLKFXKXDSISLQNEFNDEVGSAQIEHIVHTVPLINNENSSVSLLTITSRGHIRVINNANEPPYHQTTYQHILTTKEVKQIQSSQYVKEISTFFIQLEKQTLLLNTSNLSKYDRINERNPFKSKLYSFQMGREAKYTVLIYACAAAKNTTKSVLLKCFLWDNCSGQYKTFKSQTLEWQTSKDYIKDINVMPSPDXNDKVAIMSSXQVSVWNVLSDSDXEIHPITHFMAKKWFDLAKALRPMEEHLVKNMSHTPSMQLQTKTSSDDDQATAVSLSSSTLQQKSISNESWVSMQKLSNGEKKRGSKAAFAKLFQLKQKLYMLTTLGQVYQVLFKNSKYYLIQLNAFEPPECTFNDLTQINDFMISYNNNQSSHSMTNNMTVFHPLYGYNYGELSLIDQCIKQIYTTKNSTVIVLLDDSIDIYELNVDDHMDKDFTADTLLSYQHKMVFWESVLDLASFSGVNANQLLKLRDISCLWASQMLKTITAFPLHAGNAGKDTSLESMCMKKVFQKFLEFLAPPDLIIPFIDHIPPKDCKELVIPYFIEIERFCKNLHEQNFDKCIWKNQIDVDLDFFTLNNDNDEKTVKDLLKLVDTTLFNLYLNFNETLLKPFLRVPNQCDINVVIEKLKAHNKFQDXIDFFYSRKLHKQALEFMVHLEDYVPSIPLDSVMKLVFNYICQLKNENLTVIFEYLEWLFSQEWCERSPTDMCEIVFFQNRTKFDNIKVFEFLHQVDSIFSYQYLEYCVNVLXLQNSKIFFELIKYYMVQITGDQNDSLKEKTLLNLYHLIKDKPFYDCFELLSLLNDKLSTDGTKMTQRQNFILTHCKSLVLKEMNDHEGALKIIINDLDSYYLAAEYINDVRLKDDKENFNKVLKVTFNILVSQDNHKKLLLLLNDFATFFTPIYLLQNLPDDICLQDIQPFLIHNVLFQSKKNCNSTQLXKSLVQVALIENDYQALKEKSVGVSLXETSKCFVCGKLLSPGLIPDGIYYGFKMRNKDVLVHYNCKTVFQKRLDERKSIKKLPSLGEMNQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RMZ9</accession>
    <name>A0A1E5RMZ9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ribosome biogenesis protein 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g708</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RMZ9"/>
    <dbReference type="FunCoup" id="A0A1E5RMZ9">
        <property type="interactions" value="878"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RMZ9"/>
    <dbReference type="InParanoid" id="A0A1E5RMZ9"/>
    <dbReference type="OrthoDB" id="3972564at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12307">
        <property type="entry name" value="RRM_NIFK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46754">
        <property type="entry name" value="MKI67 FHA DOMAIN-INTERACTING NUCLEOLAR PHOSPHOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="60"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="166"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="40"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="172"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="193" mass="22426" checksum="73E34D0849FB0BE8" modified="2017-01-18" version="1">MSTENKELIKGFEDESKNAQDQKHTIKKLNHKNKSSSTSKKQESSGKKTKKAKQDDDLSSIIYISRLPKGFHEKELSKYFSQFGDLKQVKLARNKKTGNSRHYGFVEFVSKYDAQVAQETMNNYLLMGHLLKVEILRNSEDNEAQISKLKKYKRMIYKEDPIKKTANALKEQSNKKHSERLSSLKEQGIDFEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN01</accession>
    <name>A0A1E5RN01_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitin synthase</fullName>
            <ecNumber evidence="2">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g624</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN01"/>
    <dbReference type="FunCoup" id="A0A1E5RN01">
        <property type="interactions" value="101"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN01"/>
    <dbReference type="InParanoid" id="A0A1E5RN01"/>
    <dbReference type="OrthoDB" id="26569at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013616">
        <property type="entry name" value="Chitin_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF9">
        <property type="entry name" value="CHITIN SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01644">
        <property type="entry name" value="Chitin_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08407">
        <property type="entry name" value="Chitin_synth_1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="877"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="913"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="947"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="998"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1026"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1124"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1174"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin synthase N-terminal" evidence="12">
        <location>
            <begin position="457"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="49"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="365"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="404"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="59"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="86"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="423"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="434"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ88266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1213" mass="137660" checksum="5566530311F52118" modified="2017-01-18" version="1">MSYNYNIPNHINQPNPNEPNTQEQHQHETTYGQSYNDFLDQYSDLDAENAEKTQPQQPNDNMYNNAINNNNNNNNNGTAGLPANNHRQSSYIQQQLTRPPPPLAPSLPSRDGTDATTAFESNQNNVVIDAGPMPAITVTGVQRPSRNNTINVIPNSTTFNQPTSDLNENELFHDYSDNSSIAESADPINQRNLTSEPFISQNDPSSDSIFAYNNHTSQMDLLNHLSEETNGNTPINAYHNPSTNTNAYDNTHLSLPSSTFHQQAGFADPNLNSYELQQSFDPHGNNFSPFRDVESGEDNPIFLADQGDDYQINSYLKRNGTMVDPYKDNRDFQDIWVRRTGTLDNGHDYNAQHDFTNLANYDDGADGSNSLNDQEQFGGVGQNQHMDLGDSIDDVEKLEFPKDREVTYKNEVEEEEEETGTELSSSFHNLNGKSTSENSSMGSRSGFSRKKRSDTAVRKFLLENGNFVFDCPLSETLISSYYKSVDNPANISNEFKFMRYQAATCEPDDYIYENYTLRQQKFMVPRQTELMIVVTMYNEDEILLGKTLKGVFDNIRHMLKKTHSSTWGPEAWKKIVVCIVSDGRSKIHPRTLALMSALGCYQDGFAKSEINEKKVTAHIYEHTTKVNIAKIDDNNGIQLACDQSTIPVQLLFCLKENNQKKINSHRWAINAFAEGLKPEIIVLLDAGTKPGKNSIYQLWKEFKDPQVGGACGEIRVDLGKNCKSLINPLVAAQNFEYKMSNILDKTTESNFGFITVLPGAFSAYRFEALKGKPLEKYFFGDNMQSIKLFASNMYLAEDRILCFEIITKKDANWVLRYCKSSFGSTDVPEKIPEFMLQRRRWMNGSFFASIYSFIHMFQIWTSGQSAMRKLALTLESAYLLCNTVISWFALSSFFLVFRILTLSISVSFYKIKVFNYLAVIFLWLYGLTLLTTFILSLGNKPKGTARFYLIVFVFFAILMVYVIFCSIYLTVHTIEDLIAEKSTITFKKLLLSAPFRDMLVSMGSTYLLYLFSSIIYLQPWHMITSFIQYLLLSPAYVNVLSIYAFCNVHDITWGTKGASKAASLGKIATNKDGSVQVEIPVSAKEIEKNYQEHTHLLASQPEKEQEQEVSFDEQKQSYYASVRSIILIVWIISNFAIAAVVLETGGIQQYDRLRQTNSNVASVTVSIIGKRANIYFSVILWMVAFLAAVRALGCTFYLLRRFFYSVLHRRRNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN14</accession>
    <name>A0A1E5RN14_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Aromatic amino acid aminotransferase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g652</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN14"/>
    <dbReference type="FunCoup" id="A0A1E5RN14">
        <property type="interactions" value="172"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN14"/>
    <dbReference type="InParanoid" id="A0A1E5RN14"/>
    <dbReference type="OrthoDB" id="691673at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047536">
        <property type="term" value="F:2-aminoadipate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008793">
        <property type="term" value="F:aromatic-amino-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009074">
        <property type="term" value="P:aromatic amino acid family catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050859">
        <property type="entry name" value="Class-I_PLP-dep_aminotransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790:SF2">
        <property type="entry name" value="AROMATIC AMINO ACID AMINOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="6">
        <location>
            <begin position="148"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="567" mass="65168" checksum="DB86C24B4B37B23D" modified="2017-01-18" version="1">MVKQKENLYLQTKNNTGDNYNKMTQLTYKDFETLYQPLISSNDAKRKLNLFYLVNGLPQNLKCHENPVYLAGGMPFESMFPVQEIDLQIRDNPGSLTDDQVLSKNYSITKIKMYENEEKIKETESTKMIPVNSMIKGPSKVGLSYALQYSEVAGFPELLELTRQIITRINKPKYDMWDTLLTCGTSDSLAKICDMLIDDNSTMLMEEFTFTPVLHNLQYSGGKHIPLKLNVTSDQSKQGIDPEYMSDLLDNFDKKYPGLQKPKVLYTIATGHNPTGLTQTMENRQKIYDICSKHNIIIIEDDPYGYLQLPKYDESNPLNNPYSKGEISVDQYCKEILIPSYLTIDNDGRVLRCETFSKVGAPGLRLGFITGNKFLIDKLTKLTHLTVRNPSGVSQAVLTNMLIDMGQKYQALPGNENAPFIDGWIEWCMKISGQYTTRRNYMFQSLYEQPAFKEKKFELIEPTCGMFFNTKIVLKPEWSKKETKRIMDYLYCCLLEEGCLAVLGYKMTIDEDFSLERANFLRFTFAKANTEQLFKEGAERFSNAVERLFNEYGVDEKFDLAPQGFVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN52</accession>
    <name>A0A1E5RN52_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Geranylgeranyl transferase type-1 subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g880</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein prenyltransferase subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN52"/>
    <dbReference type="FunCoup" id="A0A1E5RN52">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN52"/>
    <dbReference type="InParanoid" id="A0A1E5RN52"/>
    <dbReference type="OrthoDB" id="3972076at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005953">
        <property type="term" value="C:CAAX-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004662">
        <property type="term" value="F:CAAX-protein geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF4">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-1 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="8">
        <location>
            <begin position="6"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ88321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="347" mass="39545" checksum="62D8CCEA4CFA775C" modified="2017-01-18" version="1">MDDPRLKKKKHIAYFDRFLGFVPASHQSMDINKLIVIYYSLCGQSILGVTELYQKYPHAQKCIRDMYYEREQFAGFLPSSLMDFGNPTNFSLPNTLFGLLCTLLLEDQHFLQNINKRKIFAFVQLCQDTIEGGFRSYIDKCGVDPVDIRYSYMAVSILHILGCRTQDDFANIINTEKLLDFIENKCYCTGTGGFSKSLEPHAGYTSCVAGIYHFLGRQCPFKDETTNWLVHRQVCNTIAVEHLNFDINDHGGIQGRENKFADTCYAFWCINSINYFGDGNIEFFDIPQVVEYLLEKTQNKVLGGFSKTNEDDSDVYHSFLGVATLGLVCGTFNGFLSLPKSICKQIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNG9</accession>
    <name>A0A1E5RNG9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein SHQ1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g839</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SHQ1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNG9"/>
    <dbReference type="FunCoup" id="A0A1E5RNG9">
        <property type="interactions" value="804"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNG9"/>
    <dbReference type="InParanoid" id="A0A1E5RNG9"/>
    <dbReference type="OrthoDB" id="73639at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000493">
        <property type="term" value="P:box H/ACA snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039742">
        <property type="entry name" value="Shq1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048696">
        <property type="entry name" value="SHQ1-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007009">
        <property type="entry name" value="Shq1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12967">
        <property type="entry name" value="PROTEIN SHQ1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12967:SF0">
        <property type="entry name" value="PROTEIN SHQ1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04925">
        <property type="entry name" value="SHQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21413">
        <property type="entry name" value="SHQ1-like_CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="2">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="508" mass="58503" checksum="4102831CFA6DAEF9" modified="2017-01-18" version="1">MITPRFQITQDEESLFIKITVASIRFNASSLEINVDENLFIFHLSPYYLRLRFDHPLIDDEDHPASAEYKLKDECIDLKIPKLNKGQVFDDLDNPLKLLARQGDILNMDKAAQPEKAAKGPLIQDITGPSLDAKNANLENIAKEGDSFDWEIKQEIQESSGSDLFKPKLGFNNQYTGEIIAMSVANGNDINEVDLPDNTNALDRCKERERKENLKFDAEYYAGEYILSLHGSAEDFEINGIKRLLKFIPELPKMYLKWYRENPQSDLSVEFTANEQNQMTDNLPHKEYFIDNVQPLYVTVVNLLYAFIFEQIENEGEHNTESAWIIGKLTPQIAALDQLLVVNDSNLEGALMKEVHQGESHNNIVRESMIIGIKRSLSYPLHRNYELSLKAWDYVYYTFLGGPKLIIKALLEIHELFRFHDVYYVYNKVLLDDLVSWFIKYNDANTISNIAFDLQTQLKSIDKNSITFDCLAGIDEETGTLEYESLSLQEIEEISNQQYQESMQSTQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNM6</accession>
    <name>A0A1E5RNM6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Inositol phosphorylceramide synthase catalytic subunit AUR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g629</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNM6"/>
    <dbReference type="FunCoup" id="A0A1E5RNM6">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNM6"/>
    <dbReference type="InParanoid" id="A0A1E5RNM6"/>
    <dbReference type="OrthoDB" id="5784at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070916">
        <property type="term" value="C:inositol phosphoceramide synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006676">
        <property type="term" value="P:mannosyl diphosphorylinositol ceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03386">
        <property type="entry name" value="PAP2_Aur1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026841">
        <property type="entry name" value="Aur1/Ipt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052185">
        <property type="entry name" value="IPC_Synthase-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310:SF11">
        <property type="entry name" value="INOSITOL PHOSPHORYLCERAMIDE SYNTHASE CATALYTIC SUBUNIT AUR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14378">
        <property type="entry name" value="PAP2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="146"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="245"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="6">
        <location>
            <begin position="176"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="362" mass="41302" checksum="3DFD31BACA0CA324" modified="2017-01-18" version="1">MEKIKRAFLKNRPSNAQCGTLDTSLNISLTLNKLKSHHFLKREIANYVILSSVLLFVLITNPASIVAKLIVVAVFALIFLIPMTSQFFFQALPIWTWLSLYFTSSTFDSSKRNPISVNVLPAIETILYGDDLSAILSNSINPVLDIFAWIPYGLFHFGAPFVVAIILFLFGPPKILRSYAFAFGYMNLVGVIIQNLFPAAPPWYEDLYEKQIPNYAMKGSPGGLGRIDQLLGISLYTDGFSNSSVIFGAFPSLHSGCATMEALFFSYVFPKLRPLFVFYVCWLWWSTMYLTHHYFVDLVVGSVLSYSVFEYTKYNYFMPKILPDGKFHSRWTYANVEYMHLQDRDPLKEGQDLEESIELNMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNS3</accession>
    <name>A0A1E5RNS3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">tRNA (uracil(54)-C(5))-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g976</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of 5-methyl-uridine at position 54 (m5U54) in all tRNA. May also have a role in tRNA stabilization or maturation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>uridine(54) in tRNA + S-adenosyl-L-methionine = 5-methyluridine(54) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42712"/>
            <dbReference type="Rhea" id="RHEA-COMP:10167"/>
            <dbReference type="Rhea" id="RHEA-COMP:10193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74447"/>
            <dbReference type="EC" id="2.1.1.35"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.35" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNS3"/>
    <dbReference type="FunCoup" id="A0A1E5RNS3">
        <property type="interactions" value="256"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNS3"/>
    <dbReference type="InParanoid" id="A0A1E5RNS3"/>
    <dbReference type="OrthoDB" id="10250660at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030697">
        <property type="term" value="F:tRNA (uracil(54)-C5)-methyltransferase activity, S-adenosyl methionine-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000201">
        <property type="entry name" value="TRM2p tRNA methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000174">
        <property type="entry name" value="TRM2p tRNA methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030390">
        <property type="entry name" value="MeTrfase_TrmA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030391">
        <property type="entry name" value="MeTrfase_TrmA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025795">
        <property type="entry name" value="tRNA_(uracil-5-)_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010280">
        <property type="entry name" value="U5_MeTrfase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11061">
        <property type="entry name" value="RNA M5U METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11061:SF30">
        <property type="entry name" value="TRNA (URACIL(54)-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05958">
        <property type="entry name" value="tRNA_U5-meth_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51687">
        <property type="entry name" value="SAM_MT_RNA_M5U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51622">
        <property type="entry name" value="SAM_MT_RNA_M5U_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01230">
        <property type="entry name" value="TRMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01231">
        <property type="entry name" value="TRMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 9 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 9 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TRAM" evidence="11">
        <location>
            <begin position="136"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="576"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="576"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="448"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="481"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="502"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="549"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00052788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00070108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ88516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="624" mass="70193" checksum="2734AB78C6DE8BD8" modified="2017-01-18" version="1">MFRLASQLKRGSKVTNLFLLHRNHTSTSLCKPKMTEVSANATAQMAKSSKNESIESNNGPNLKKIKLENAMGAKKANNSRKKLRKFKPKKYDSTSPLGVLQKEIEELLQEHGLKESDVSNDVALVINNENKAEVDAVYHTIVQDVNVLKFCSNGDGLALIPGSPFTDRTHRIVTIPFGIPGDVVTIKIFKTNQMYSESDLLEVQKPSVMRDDSLIKCDKYFGKCSGCQYQNITYQTQLSLKRQTIENAYKFFANSSVNPESESCELSLEKYQEMYASKIRDTVGSPLQYDYRTKLTPHFDISFSLSKKLRKQAQNGSTTTTTEKIEFERPNLGFGVKGKPTWRKFADPSVANCKGSVLDIEECVIGTPVVQEGLKRERNKFLNEYKNYKKGATILLRENTEFVDQQEKEEVANADSKVVKTCVTNPKQIVVELVDGFKFEFIAGEFFQNNNSILPLVIDHVKKNLVISQDSSKPRYLVDAYCGSGLFSITCSTNVNKVIGVEISQEAVKFATRNSELNKIGNCKFIAGEAQEIFKEIDTPADQTSVILDPPRKGCDEVFLKQLSEYNPAKIVYISCNVHSQARDVDWFLKKSANGHLYKIDSIQGFDFFPQTHHVESVCVLSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNT7</accession>
    <name>A0A1E5RNT7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g771</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNT7"/>
    <dbReference type="FunCoup" id="A0A1E5RNT7">
        <property type="interactions" value="93"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNT7"/>
    <dbReference type="InParanoid" id="A0A1E5RNT7"/>
    <dbReference type="OrthoDB" id="191979at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF285">
        <property type="entry name" value="RETINOL DEHYDROGENASE 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="281"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="371"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="379"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ88544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="410" mass="46110" checksum="A86F8C4F1A10FBEB" modified="2017-01-18" version="1">MPVNIIGSLIIDGPDAIPYYEQITKVLPYATGIGVVKYLFKGRKNTWERNLHGKVYIVTGATSNGMGTSVVLDLAKRGAQLIMLTRRVDEWTTQFVEDLRESTQNELIYLEQCDMSDLYSVRKFATTWLDNTPPRRLDGVVLMHGDAEPWFQNDKRKSSQDGVELQLATNFAGPFHLLNLLLPSFRSQPPDRDVRIIYTTCISQAFGSVNLDDPLLEKKQLNARQFFGTAKLQMSLCCLELQRKITSEKKDDAGSVTVSLVQPGLMRSSSLRTILSNGSPILLLLLYCIILYPLLWLFVKNGHQGAQNILYTLMTPELEKVNLLDYSVKYVVNCTIMKNFARKEFADQELQKKLYEKMESEILGIEKKSAFRRNKAKASGSTKEAASTSGTKSANNTSSSKPAKRKSKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNU3</accession>
    <name>A0A1E5RNU3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">mitogen-activated protein kinase kinase</fullName>
            <ecNumber evidence="8">2.7.12.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g968</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.2" evidence="8"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNU3"/>
    <dbReference type="STRING" id="56408.A0A1E5RNU3"/>
    <dbReference type="InParanoid" id="A0A1E5RNU3"/>
    <dbReference type="OrthoDB" id="10252354at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038066">
        <property type="term" value="P:p38MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06622">
        <property type="entry name" value="PKc_PBS2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000341">
        <property type="entry name" value="MAP kinase kinase PBS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013:SF25">
        <property type="entry name" value="MAP KINASE KINASE PBS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="853"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="61"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="199"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="391"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="487"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="704"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1145"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="61"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="199"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="241"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="253"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="304"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="361"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="391"/>
            <end position="404"/>
        </location>
    </feature>
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        <location>
            <begin position="408"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="503"/>
            <end position="521"/>
        </location>
    </feature>
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        <location>
            <begin position="531"/>
            <end position="546"/>
        </location>
    </feature>
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            <begin position="611"/>
            <end position="622"/>
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    </feature>
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        <location>
            <begin position="704"/>
            <end position="728"/>
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    </feature>
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        <location>
            <begin position="738"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="882"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038035"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ88549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1165" mass="126832" checksum="67402E7351627D2D" modified="2017-01-18" version="1">MDHAPANDSGGNVNMPTGSNDSDHNTAQRPSLQAHSSSNPSVSSSKISQNLQARVLAFQEQRQQLKRSTSQKTTANAVGTKPQPMSPTVTTPASAPPNPHYNASQQLLQHQHSRLDSTLSPNPQLPQTHEPSQDPFFNNASHTKNVEPVSQYPKTLSDSENIALSSLHSNNSRKRSDGPNASNNSNLQVFTNTHTLYDSSQSSASTVSGNNTPNSLSLSFTKTPNMTIVNKPLPPLPPPSRSNSVTSSQHAVSRSSSLNNNASINNNNNHLNADYSSTSNSSSNLNINEKDGLNEDFNSSMRDLSVSSNSNHSNNNSTLSLQNVPSRNSSLNSKQQNSNNAGEQLNNGGLYSSHHNTNSSHKLDLREDSIRSDSTESNLTLKESISRRILSRQSTLKGDSQKSQQLDKEEEEEEEEEEEEEEEENQGQGQGQGQGQGQRHQEAEFDIGTNYAKRATRAELNNVDNQLLQKGKEYAASNNSRQSFWNDEASANNFLPTPDLRIVTSNKTQSNSSMSPTSAQKFHNYRDSEDVSSTGSTSRPTTPVSGNFRKLAKQLPGPLRKAPPIPKNMPLQLKNSIQNGTGGGGEVESNMNTGAKKSLYPLPMPKKPVFQQTSSPQSQQKSMGSDEKPKPKMKLGLSARRGMKLDLSSMNNPVAGLNQKSVLNPVLSMKMNKNTNSPALTSSNTNYLDNDSASNLSCANFSRGSNTTTTSTVISNGSTSSSSSSSDGGNSGLHPPNFTGSLISSTFNSHSTNSRNSLHLNSTRSLNMGTPNNSIMNNPAMGNMQHNTNSSGGGSNVNSMGSINNKSMGLFANFSKYVNIKSGSLNFAGKLSLSSKGVDFSNGSSFRITLDELEYIGELGRGNYGNVSKVLHKPTNIIMAMKEVRLELDESKFRQILMELDILHRCNSPYIVDFYGAFFIEGAVYMCMECMDGGSLDKLYQALPNSEGIDEPRLAYIAISVINGLKTLKDEHNIIHRDVKPTNILCSAMQGTVKLCDFGVSGNLVASLAKTNIGCQSYMAPERIKSFNPDAATYTVQSDIWSLGLSLLEMALGKYPYPPETFDNIFSQLSAIVDGQPPRLPAGKFSPEAQDFISRCLDKHPDRRPSYAMLLEHPWLKKYENVDVGMKNYLTARLQKISEYQEANNVNSNDEVQTRPLPALHKGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNW4</accession>
    <name>A0A1E5RNW4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g667</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNW4"/>
    <dbReference type="InParanoid" id="A0A1E5RNW4"/>
    <dbReference type="OrthoDB" id="3973436at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184825" evidence="2">
        <location>
            <begin position="25"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="106"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="250" mass="26643" checksum="719FCF9C0173FD26" modified="2017-01-18" version="1">MTLFSSYFFSLALSIMTLFSVANAANHKAPKQDHHLHKKGLITSDGIVYSEYFVTSTLPPVTYYYTSYWTSYYTYAITQYSTFTYGDSSTVTKTYIAPTTSVSVGTTQPASSETTTSSSTTSTLTPSTSSTSPTSSTSSTSSTSSTSSTSSTSSTLSPTSTSNSPSTTSFSSSLAASTTNLFEAVAEPYLVTDTSDSTATKVYYYEDIYYDDSGSATSTVFEKFIGSGTPTISVYSTTTSTKITYITITV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNX7</accession>
    <name>A0A1E5RNX7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DNA mismatch repair protein MLH1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g912</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="interactions" value="769"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNX7"/>
    <dbReference type="InParanoid" id="A0A1E5RNX7"/>
    <dbReference type="OrthoDB" id="10263226at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032389">
        <property type="term" value="C:MutLalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061982">
        <property type="term" value="P:meiosis I cell cycle process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16926">
        <property type="entry name" value="HATPase_MutL-MLH-PMS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000003">
        <property type="entry name" value="DNA mismatch repair endonuclease MutL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000014">
        <property type="entry name" value="DNA mismatch repair protein Mlh1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014762">
        <property type="entry name" value="DNA_mismatch_repair_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013507">
        <property type="entry name" value="DNA_mismatch_S5_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032189">
        <property type="entry name" value="Mlh1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002099">
        <property type="entry name" value="MutL/Mlh/PMS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00585">
        <property type="entry name" value="mutl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH, PMS, MUTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073:SF12">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01119">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16413">
        <property type="entry name" value="Mlh1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01340">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00058">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair protein S5" evidence="7">
        <location>
            <begin position="214"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="418"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="426"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="439"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ88579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="615" mass="68949" checksum="AEA6DEE8B2D2DCFB" modified="2017-01-18" version="1">MSIKPLDPEVVNKIAAGEIIISPANALKELIENSIDAGATKIDVTVQNNGFKLLQISDNGSGIAKDDFPILCHRFTTSKISSFSDLSNIQSFGFRGEALSSISHIAHLSLITKRNDNKDGDLAWKAVYEMGELLPSSVKPSAGNQGTIISITDLFYNTPSRLKSLNQKEEYHKILDCLGKYSVNNHGIGFTLKKADESKMALIIKSTTPVLDRIRSIFGSSIANDLIAFEIKDIDELVEELGFVSCKGLVSNLNYANNSNRKAAKPVFFINNRLIDCEPLKRAVYQIYANYLPRGAPRPFVYLNVCLKPDHIDVNVHPTKKEVGFLNEEEIVTFISDKLGELLSKQDSSRTFKANAPTTNTLSNVHIINPSQAPFGMSQGAQIKRDETRLVRIDSQQSKITSFLKSADVKYESQSYGSTQSKVFGEEEEEVEAKEEESSLNTNSNFNSQSQQSLQRSSSENLLNKLVFNEEREKNQVNLTSIENLKEVVNLNAHSELTTILANLVYIGVIDPYKRLCAIQHDLKLFMIDYGSVCYELFYQIGLREFANFGKFYLSDCLKIRDLLELLNVLEAAKITEIVEQLFEMKDMLDEYFAIEIDKMENSDEVVIKSAWSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RPK7</accession>
    <name>A0A1E5RPK7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cysteine protease RIM13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g929</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C2 family. PalB/RIM13 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RPK7"/>
    <dbReference type="FunCoup" id="A0A1E5RPK7">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RPK7"/>
    <dbReference type="InParanoid" id="A0A1E5RPK7"/>
    <dbReference type="OrthoDB" id="167576at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051297">
        <property type="entry name" value="PalB/RIM13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46143">
        <property type="entry name" value="CALPAIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46143:SF1">
        <property type="entry name" value="CALPAIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="7">
        <location>
            <begin position="102"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ88623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="751" mass="84602" checksum="813EEB85D511B4AA" modified="2017-01-18" version="1">MDIYDLQVNSNRKGGGGGGGAQQSNSQTQANQPPLWSDLNDIRWQIYTSSSQNTKNTLSVSIVGPKLKDNCPRALQKALLKLLQCHAELPLQERFLWLSSFYLQRAYPPISLEETGGYTPHFLIEETGVSLDSKDFPEYKINKPLLSSSAPKTGTPNLIRQHRKIGDCSFVCSLINLQRANELLPYLASKNVPGTYMVNFHFNGSDDRLVCTTNVAHIPTTDSNDQLGLYSSEENHKILELAYLKLKGSSYNHIGSNTAIDTFLLAGFIPETLKIRDLASWAKLLQFYKSGLCTVAFGTKAAFANASCEFISGHDYAVHDFSGSQISLTNPWAPEVPIVIKQWSTFTEYFDVAYISWNAKKMFKFHEKIHFSYNAETNNKSIRSTLNKPAFLIANDSLNNETVYVLLEKHLSQEHPGIHLELVNDSIDVSNLTKYKNNTGFHLLKVSIDSITEKRVCCFSELSVRFTLHIYSLSKSITIKKANDKNVISVTNVFLENYTFENDKYCMNQTYKLDFADANDENKHCNTDGSENVVLCTVQLLATKSHINFQLYHLNDFALERPLISEHAYEFEMQTSPQIPLARNKQYKLICSAKDAYCGAEFVLGCSTNKRSIRPKLSEYYNYFGGLFKTSFSATDFVVKDIGDGTKLSVLLKSEMGSCHDMWNIRLFNVSCKKKHLDWSIRVVDQEYGHSLLSNSSGCSTFTSNTAANVVVNKNGAVSRKVTFEMTLLEKIDPRSTHLLMEIGTQYKLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RPN6</accession>
    <name>A0A1E5RPN6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LSM12 anticodon-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g734</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RPN6"/>
    <dbReference type="FunCoup" id="A0A1E5RPN6">
        <property type="interactions" value="689"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RPN6"/>
    <dbReference type="InParanoid" id="A0A1E5RPN6"/>
    <dbReference type="OrthoDB" id="1057137at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ88663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="120" mass="13801" checksum="751537F2432C1410" modified="2017-01-18" version="1">MNLDNVIGVNVKVTNVINESSVGRIYSWNTDSNTLTLLTNSPKSNGKQQQQQQEPFFSFKLFKISFIQKIEVIPNQSQPSSQAYLKKEPLKPHHVYPERIENFINGEIKKYREQNSMATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV16</accession>
    <name>A0A1E5RV16_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g417</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RV16"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="561"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="660"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="784"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="834"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="957"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1047"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="176"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="334"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="564"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="669"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="683"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="729"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="784"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="845"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="862"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="1101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1102" mass="119720" checksum="A94EBA32B4B29AEF" modified="2017-01-18" version="1">MPLLPSSRNSNNHTVRGTVSASYASNTGSTNNASSVPQIEKSSEPSQGSSASSSRWRLPHYRRHHKLKSSISSPMLPQTSTSNGGKLNVETGFNVTSNNAIDDLNDSLAKFKGYMHQSISENPSPVNSVGTSSTNSTNFSSISADTDNQNLQSKFSNNDVETVGNQQSNQIKSSNPHTQTQTQTQTQTPAHAHAHAHHHHHHHRTSRVLKLFKYKHHEHGSCSSDDISTCKEGFGTANDERNLDVQASFASLKYQQQASELEDPSKQKKTKRSYSSRILHYATGNSLRSKNSGASPINNNGQNNATFFLESAPNDANLDYHQNSSMQTSKTGLVESPSAETSTSPSVPRNPVFVENFQSKNTSSPCVIDGQTMTSFNNSNNQTQXPSPVIYSTRAGSSPLVDQHYAMNMQVYSRPTSALKRSKSYTISDPPYSPYGFNHTNNKSFXTMDGTSPAYAASTLSASSARFGDSSDSTFSPVLSRFSQAPIIHSPLNTSTSAFPNACSNMINNKXNMVAGSTNESSTNKNPRFKRSNTFGETSLMNMQLQQQQHMKQGLLAKSLTTESRSASNTNTNITSNSDTQDDNELFEYGETESAKNIHIIADXDDENDASVAFNKVFSSASNSFSNLNLPSAPNSGTQFLDSVPTPHTNKHTAHSAAGTLAGPLHMSTHSKPHYISRRKRTNTCGSTNSRTSISTNASSVVKLSPIRRSTSPNRSVGNTTLNTGSNVSSLKSQHQQQQHWSSISSGSSSIMSSNIRSNSSSNLLQISTASSIYAPNTANNKGLGTDTYLDQNSNVQNNLGGNSQPLPPVPQLLNTTAHVSTTQPILQLPSSVVRSSKSRMLKNAITSSSSAGPQKTEKSRASTQNKMNSLSMRPGTKHTDLEMQGYPNPSDEYLQPPNSATTITSAGHEMPMDDERTVYSHSTTTPVDLSQGSGLADVGEDPEFMNLMSLMEITHPSSSEELSSAVDGKNSQNAPQATVQELEDLESELDLNSVFKHKDGENASGNTGPLADNMENRRYFTSDALDRHKDDVLMLQDNGIEELSHNDDSKNNFPHTHSGSEFPLGRSGNESDEMMQMMLDLPELPEDFDQDYFLINETEDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV46</accession>
    <name>A0A1E5RV46_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g477</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RV46"/>
    <dbReference type="InParanoid" id="A0A1E5RV46"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184891" evidence="2">
        <location>
            <begin position="22"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="91"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ90812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="178" mass="17901" checksum="349576DA10CBA9EB" modified="2017-01-18" version="1">MYTKVIASIALLTSVAAAADASSVTKKVTTTEEVTSTGTMWMTMPMLDTKGGSTVTEEMGMSMFMTSVMEVPTTYTTVVAASDDSMSSMDMSSMDMSMNMTSASSSSSSAKSTSMSSMDMDMGSSSNSTASTSASTSASASANSTSSASISSTKSKNGAVGQNLANSGLVALAAIALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV47</accession>
    <name>A0A1E5RV47_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Cytochrome c oxidase assembly protein COX15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g462</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Fe(II)-heme o + 2 A + H2O = Fe(II)-heme a + 2 AH2</text>
            <dbReference type="Rhea" id="RHEA:63388"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:60530"/>
            <dbReference type="ChEBI" id="CHEBI:61715"/>
            <dbReference type="EC" id="1.17.99.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:63389"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Porphyrin-containing compound metabolism; heme A biosynthesis; heme A from heme O: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RV47">
        <property type="interactions" value="894"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV47"/>
    <dbReference type="InParanoid" id="A0A1E5RV47"/>
    <dbReference type="OrthoDB" id="3969963at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120547">
        <property type="term" value="F:heme A synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016653">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006784">
        <property type="term" value="P:heme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01665">
        <property type="entry name" value="HemeA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003780">
        <property type="entry name" value="COX15/CtaA_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023754">
        <property type="entry name" value="HemeA_Synthase_type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23289">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23289:SF2">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02628">
        <property type="entry name" value="COX15-CtaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="215"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="310"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="384"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="414"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="443"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ90827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="501" mass="56288" checksum="E41B14A1305B8506" modified="2017-01-18" version="1">MFRNIVSLKQGVRALSLFNSTASKNARISTSTATLFSKSFSPSLKSMTASVXTSISSKSTLQRRFFQTKNSKLYSTVQEEAQSARPQRKTRRLMNSHSNVGYWLIGTSGLVFGIVVLGGLTRLTESGLSITEWKPVTGTIPPLNQKEWEEEFAKYKDSPEFKQLNSHITLDEFKFIFFMEWFHRLWGRAIGGIFVVPTLYLLASRYTSARVNRRLLGLSALLGLQGVIGWWMVKSGLDEENLEERKSKPTVSQYRLTTHLGAAFMLYMGMLWTGWEIVRENKWMKNPXESIKIFEKLNNPQLKPLKKMSLALVALXFLTAMSGGLVAGLDAGLIYNTFPKMGDTWFPSKRELLDKNFSRKEDNSDLVVRNVLDNPTTVQLNHRILAITTFTAVLAFHMYAVKRKALIPKNVNRTIHAMMGLVTLQAALGIATLVYLVPISLASAHQAGALALLTSTLLCASQLRRPRPIMQQLIMNLQKNGIPQKTGSKILRQINGMPMKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVD1</accession>
    <name>A0A1E5RVD1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g419</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVD1"/>
    <dbReference type="FunCoup" id="A0A1E5RVD1">
        <property type="interactions" value="933"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVD1"/>
    <dbReference type="InParanoid" id="A0A1E5RVD1"/>
    <dbReference type="OrthoDB" id="7851174at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23813">
        <property type="entry name" value="UBCc_UBE2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000020">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="8">
        <location>
            <begin position="4"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ90775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="150" mass="16924" checksum="E1D5826369B6C036" modified="2017-01-18" version="1">MSNPLPKRIIKETARLVSDPVPGITAVPNEENLRHFQVEIQGPSESPYAGGLFKLELYLPEDYPMEPPKVRFLTKIYHPNIDRLGRICLDVLKNNWSPALQIRTILLSIQALLASPNPHDPLANDVAKEWLDNESAALQKAKECTEKYAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVN6</accession>
    <name>A0A1E5RVN6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">mRNA decay factor CTH2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g319</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVN6">
        <property type="interactions" value="78"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVN6"/>
    <dbReference type="InParanoid" id="A0A1E5RVN6"/>
    <dbReference type="OrthoDB" id="3972987at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF18">
        <property type="entry name" value="PROTEIN TIS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="162"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="200"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="162"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="200"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="119"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="261"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="119"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="263"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="8">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ90919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="300" mass="33821" checksum="71D30D16057E5A4F" modified="2017-01-18" version="1">MNSYNKGSVYSSIPFVNVEYQRESNKKPLNFNTFEVAPLQKNPAAMDEHYVYQNSDNGSITPPQYPVSVSSASNINVEQNNNKLLSPVSIDNEVARAQKKLQEAQDFLKFLEQQKKVQQISQNTKSTTNHHQXQVSAAPRSPKHARANPQRKIEINKINKSLYKTELCESFVTTGKCRYGNKCQFAHGLSELKLKDVTNNYRTKHCSSWIKYGYCKYGNRCLFKHGNDEDIKVYLAAGTLSNGGPQTDTLKTQEHTHATKNFASASPSVSLSSSSARSSNRVHHNNFANVKTIKKLNESW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVN8</accession>
    <name>A0A1E5RVN8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">26S proteasome regulatory subunit 8 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g513</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVN8"/>
    <dbReference type="FunCoup" id="A0A1E5RVN8">
        <property type="interactions" value="1113"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVN8"/>
    <dbReference type="InParanoid" id="A0A1E5RVN8"/>
    <dbReference type="OrthoDB" id="1664597at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000006">
        <property type="entry name" value="26S protease regulatory subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000044">
        <property type="entry name" value="26S protease regulatory subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000030">
        <property type="entry name" value="26S protease regulatory subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="166"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="12"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ90838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="394" mass="43821" checksum="839DAAC0146A177F" modified="2017-01-18" version="1">MAGVKPFFEAQIQDCEILIRNKQENLHRLEAQRNLLNDKVKFIKDELVYLQEPGSYVGEVVKVINDKKVLVKVQPEGKYIVDVASNIDIKSLKVNQRVCLKSDSYVLHKVLENKVDPLVSLMMVEKVPDSTYDMVGGLTKQIKEIKEVIELPVKHPELFESLGIQQPKGVILYGPPGTGKTLLARAVAHHTDCKFIRVSGAELVQKYIGEGSRMVRELFVMAREHAPSIIFMDEIDSIGSSRGDGGSGGSGGGDSEVQRTMLELLNQLDGFESSKDIKIIMATNRLDILDPALLRPGRIDRKIEFPPPTVAARTEILKIHSRKMNLTRGINLKKIAEKMNGCSGADAKGVCTEAGMYALRERRIHVTQEDFELAVAKVMNKNDETAISVAKLYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVQ0</accession>
    <name>A0A1E5RVQ0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">F-box protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g356</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVQ0"/>
    <dbReference type="FunCoup" id="A0A1E5RVQ0">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RVQ0"/>
    <dbReference type="OrthoDB" id="4060589at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ91011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="616" mass="71959" checksum="DB9FA9508F5B5F52" modified="2017-01-18" version="1">MLDCIPDDVAAYFWQFLSQQDRVKLTTLNRSSYKIYSQKLYKNLFLNRLPVYDSDTDINDFKIFTKWSYLVGSLSTDDLRSEKNGSNPNKLLQLNNESLRADFIDPLSLLLRTFSKNPSLLTHVENVYCTWHIDKKLKYQLLDLLAECVGHANMMKGEVALLRGGNLISFENNLDFKVFEKLHLNCTFEQNRWTALDIPPITPLPNSIQDIKKKKKYLSTAITVLSQSAFQNIQTLTLHFNPIHVFPLLNKKLVLNVKNLCLNFRPDKLENEHDLDQSKTARLVDFFNPDYLEKLDLVSWHETTHKNFYQKNGLLDAVVNFPNIKHMKLASLPYSETFVKLIFKHWNKLNYLHLDFFIEPNNFNHSFFVDHKPSFHDSLHYLFVRISDELVPKRYSVTETNAQGAVYMKPAATSCLCKSCVKVHEEIIEKYYFRSNKTLIPINEKTVSQKDYTNHIFQRFSILPYCANVLNEDYPPVGYGTHTLSQCSKKIHELHSAALQKSCNKLNWWEIRALYFYTYHQYARSFNTFLGLFRNLRVLNINDVPSRIVGSRNYPLFLTQNTNYTSNQGVDPTEIAHKTLYEIESVTTQLDSFDVEEELVREPSLNSDSDDSDFDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVQ5</accession>
    <name>A0A1E5RVQ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Mannosyl-oligosaccharide glucosidase</fullName>
            <ecNumber evidence="11 12">3.2.1.106</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Glucosidase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g541</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Cleaves the distal alpha 1,2-linked glucose residue from the Glc(3)Man(9)GlcNAc(2) oligosaccharide precursor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(4)-(alpha-D-Glc-(1-&gt;2)-alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + H2O = N(4)-(alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:55988"/>
            <dbReference type="Rhea" id="RHEA-COMP:12806"/>
            <dbReference type="Rhea" id="RHEA-COMP:14355"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:59082"/>
            <dbReference type="ChEBI" id="CHEBI:132537"/>
            <dbReference type="EC" id="3.2.1.106"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="13">Glycan metabolism; N-glycan degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the glycosyl hydrolase 63 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.106" evidence="11 12"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVQ5">
        <property type="interactions" value="592"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVQ5"/>
    <dbReference type="InParanoid" id="A0A1E5RVQ5"/>
    <dbReference type="OrthoDB" id="410058at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004573">
        <property type="term" value="F:Glc3Man9GlcNAc2 oligosaccharide glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.110">
        <property type="entry name" value="Glycosyl hydrolase family 63, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031335">
        <property type="entry name" value="Glyco_hydro_63_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031631">
        <property type="entry name" value="Glyco_hydro_63N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038518">
        <property type="entry name" value="Glyco_hydro_63N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004888">
        <property type="entry name" value="Glycoside_hydrolase_63"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10412">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10412:SF11">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03200">
        <property type="entry name" value="Glyco_hydro_63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16923">
        <property type="entry name" value="Glyco_hydro_63N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184900" description="Mannosyl-oligosaccharide glucosidase" evidence="14">
        <location>
            <begin position="20"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 63 N-terminal" evidence="16">
        <location>
            <begin position="46"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 63 C-terminal" evidence="15">
        <location>
            <begin position="335"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 63 C-terminal" evidence="15">
        <location>
            <begin position="836"/>
            <end position="892"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU369107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ90905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="899" mass="103127" checksum="2E13FCB1767F425A" modified="2017-01-18" version="1">MNLIHCLVAVAFLCPFLEAYSAASDSKKLDVANVESKQFHQRLNQSLLWAPYRSNCYFGIRPRYIDATPWSSGLFWFQSASSAPVRNAAQFQKHFLKNIRHFVEQGDNLXRYNWELYDPXLGGKQVLIDSKNNINLTILWSKSSDGNNWGYKVIGQPIAEPHKNPTSLSMIMYMSQASKEPTDYLMMAPSKFADTQDRANKMSFVGSSSELKDYEIEIYDVRGKYHESLSDADAFPQIDTSKPVHFSMTVPEEDTWKAKDIFQLLVSDSVSNIMQNLQKTPKNIDMAAMQTIRNVHDFEKGNFHFVQKTFDLSEPFEFDVIYNSEHSSEKISGSEELTELIASNLALIKSKYERKIVAAGNGELGFSQEEKDFAQHTFFNLVGGLGYFHGSQRVDRDTELLDHSFQDIKWENVQMEGPYSLFSLVPSRAFFPRGFYWDEGFHLLQLLEYDFDLCFEILQSWFNQIDEKTGWIPRELILGDEARSKVPEEFQVQSPQVANPPTLLLVFSKILKQAVDAASKRGNSTSAETNFDTEHLKKDASLLLEYSKNIYPKLLKHYQWFKETQKGCADEYFAFFEEEGYDMNAVNLHELYRWAGRSLDHCLPSGLDDYPRADYVDISEMNVDALAWVGVLSRSLKQLAEILGYQDDVAEFSKHEQEVINNLHNFHWSSTHKAFCDVAINPYTDEMEHICHEGYISLLPFALKLLPKNSSTLPYLVELMSDKSKIFSPYGLLSLSKKDKYYGTNENYWRSPVWLNINYLCLDALNYYFGSGAESLSAQEGETKYDGEMLXEIKKKMXDNVVPNGPLGFAKSLGNQIAGKREDFFESEEASARCHLEKARVLYKDLRKNLXKNVFKVWKKSGYVYENYKAEDGAGGGVEHFTGWTSLIVNIMYRLPAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVT1</accession>
    <name>A0A1E5RVT1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g564</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVT1"/>
    <dbReference type="InParanoid" id="A0A1E5RVT1"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="162" mass="18502" checksum="69CEB361744E9F66" modified="2017-01-18" version="1">MDRGAGKYKDGDTCSNNHNDHELKVDNTDVIESGTDTAGDRSKQIPEFSNTLTQKVLDEDLTIDELMNEVAAILRRDCGSTHDLKTAFCGSEDDEKTDIHEAIIARRYSPLKKREGVWAITRGEQEKDRIVTMTTWRKQNRRKVSMLNLCFEHVKTRTARTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RW02</accession>
    <name>A0A1E5RW02_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RW02">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RW02"/>
    <dbReference type="InParanoid" id="A0A1E5RW02"/>
    <dbReference type="OrthoDB" id="7042322at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047536">
        <property type="term" value="F:2-aminoadipate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000080">
        <property type="entry name" value="Aminotransferase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42858">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42858:SF1">
        <property type="entry name" value="LD15494P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="1">
        <location>
            <begin position="51"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="434" mass="48793" checksum="ED6F44BAC5408853" modified="2017-01-18" version="1">MKASTEEYNFYVGHPTDNLLPNKEIVKATAELLTPDHRDIDDDPLELHPLNYGTDIGSLWCRKEICNLVNSYYPAADCDPEFINFNNGASYGIMNILELATQPHAYTKQAFLVSPTYFLMNKIFSDAGFEGKMTAISEIGDGTTIDLEKLEKKLKSCEANCEAECRETDALNTQNTENFYKYVMYLIPTFSNPSGNTYTVECRTKLIELARKYDMLIISDDVYDFLNYSTEKTNLPPRLVHLDRKTLXKENKRGNTISNCTFSKLIAPGLRSGYXECATLKMAYFLAEGGANHSGGTPSQLTSNIIGTLIQNGDFAKILDNLESVYSKRSKTLKNSIDKYLPVGTQYKQTKGGYFSWCTLPDQYNCHKVCKIAKEKFNVVLANGDDFEVTSDKYGWGSKSVRLCISLMSEDDIEKGVQLWGEACKLSLKCESNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZB4</accession>
    <name>A0A1E5RZB4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetyltransferase SLI1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZB4"/>
    <dbReference type="FunCoup" id="A0A1E5RZB4">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RZB4"/>
    <dbReference type="OrthoDB" id="3972366at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052058">
        <property type="entry name" value="Alcohol_O-acetyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010828">
        <property type="entry name" value="Atf2/Sli1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28037:SF2">
        <property type="entry name" value="ACR018CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28037">
        <property type="entry name" value="ALCOHOL O-ACETYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07247">
        <property type="entry name" value="AATase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ92297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="504" mass="55982" checksum="2E29CFCDB315FCC8" modified="2017-01-18" version="1">MSYSHSSKNTHTKTSNTNCFHSMTKDLEPSVTPGFKAYLPFVHKYFYFRTCSELSTAVYVGVQYNKTPSVAELYEALAKVVDKNPQLGLQIFADDGADVNCCPVEAEDGITKPDPPSRVYAAQLKMLDFSKVVSFHEELDAKSDEAITMFLGERFEFGSSDKSFWKIDVFKDNWLVFTFDHTLLDGMSGVLVQNDLANFLNTGHSGNVQRCNNIYRPKKIASSVEDLGVVNIYTKPHTDRAGFAHHLSKSLVSEKYTFNPEHVIEKNHFIDNTRYVSIPQSDLLRLIRVGKQNGSIALNSLLCALLGVASKNTFVLGEKTIPFLFAVNHRESSNLPADLLGIFIKAEHLTSPILKDSLFQANELNIAEFLQYAADVHGLVAKAIISKDGLENFNELALVDLLSATKGKEGKLPTDVFASSNLGAQFKSYVSGGDRGLAFTIEDSIFGQSHSFSNLLTISSISTLNGGMNMLVTYPDNRKKEGDMFVENLNRCIRALLDFELREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S035</accession>
    <name>A0A1E5S035_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">GABA-specific permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5S035"/>
    <dbReference type="FunCoup" id="A0A1E5S035">
        <property type="interactions" value="191"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S035"/>
    <dbReference type="InParanoid" id="A0A1E5S035"/>
    <dbReference type="OrthoDB" id="3970180at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF6">
        <property type="entry name" value="GABA-SPECIFIC PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="318"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="372"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="430"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="455"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="496"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="529"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ92419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="587" mass="64299" checksum="339436A0CD0E2D53" modified="2017-01-18" version="1">MSDTTSYERNEKHNGKAPEANVVVSNVSDARHLRTVTSTRGYQMQVVRSQDQQVALEELGYKQELVRKYTWFELFGVAFSIMGLLPSIGSTFYQAISAGMSGATWGWLIASFCGILPMGIGLAELGSAYPTASAVYLATWNWSPPKLRNFISYGVGFIDTLSLAASVCSITSGCSGQILSCVQLYKPDFVVTNGNNYGVYAACIVAMACIGSASSGFNAKVQTISTISNGFLLILVFIALPAGTHHKGIAFNGGKEIFGNVENFSNWPKGWNWILNAFQPAVWTIGAFDSCIHMTEEARFTPRKPGRPLLLDPAASPAAFGIITSIVVCGLLGFLMLICLCACMGPSVADIYNSSYDEAITQIFMNSLGQKWTVAIMSFMCWGSFLMGASCMLATCRQFYAMARDYGIPTKFLSEYFAVVDSKLHVPVRAMWGSTALSLALGCMMFGGDACSAALFSLSICGMYMAIVVPMFLRLTYARKDFVPGPFYLGNFWSPIINWIGVLFQIFMIVIVCIPLVNNPDKTEMNYTVVIGPGFLLLGLIHYSLYQHKFYHGPCSNLTDEEYAELMIDENIDAIISSDKIDKKEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S051</accession>
    <name>A0A1E5S051_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein ROD1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g119</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5S051"/>
    <dbReference type="FunCoup" id="A0A1E5S051">
        <property type="interactions" value="110"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S051"/>
    <dbReference type="InParanoid" id="A0A1E5S051"/>
    <dbReference type="OrthoDB" id="2333384at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF17">
        <property type="entry name" value="FI21816P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="2">
        <location>
            <begin position="293"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="478"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="545"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="642"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="872"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="918"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="872"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="954"/>
            <end position="963"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ92504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="963" mass="105109" checksum="8B71CB82096E3F17" modified="2017-01-18" version="1">MLSFVSNKSSSKPPVLFEIRIDKNNSSTPSGNNDVLIFKGPPEEAPSILLTGNIVLSITEPMHIKSMYLKLTGDVRIKVPLTFHTQKGEPITRLSRFDKRFYEHTWGNKNFKDHLSKMNTSSTNSANISSVNSSNNLQASNQFQSIDKPSSHENANVARPDTLSKQKKAKSTTNLKRFASSFSSTALNGSTTSLSTINKPHHDPKGSATSSAGSSHLLPKGNYEFPFSLVLPGNTTESVEGMPHACCVYKLQAVIERGRYLSDLQVKKHIRIIRTHAADSLDLSETVAVDNCWPKKVEYTISTPAKAVIIGSSIPIHMAFVPLAKGLKLGTIKCYLLENSAIVSVHGDPYQQERIIAKSKTPNPLKTNRDANYFQHSDYQYYLNESEQQAEAAGSGRDDQGEFQDKWEVTLFLDTPTSLSKCTQDCTILTYMRVRHKLKFVISLINSDGHVSELRASLPIQLFISPFVPISCKSVDPLDTASRTSSAPTSDAEDEGYNGNGVSKRHNQSNNTQGEPGDDTVLFDSSTNLLYDSVVNAINSKSSENLAGSQSTQDQQSLPALLHSKSSPQITTPLTRANGSRHGSNASGLNMMSTTPINSTADLLAYSRQHSSMVLTNLLAPPNYEHRVYDKLWDGVKYIESGQTTPKGKTPLKSNGTANQTSGNNGNFAEQNSSALDLGALQNGLQRLQLERADSRQGNSTPPALGTNFPPALSNDYFSKMSLTNVPTVSHLSRTTSFHASEPQTLSGVSADEASNIKKWKNVGSLAKVPSYKDTMKSATAGSQIDLPPVYDPQDYEKQPAVLGDDFLKRPKQSRVRGGSNENMPTLGLSALRRNNSSGLSMYRTNSANSTDNISDNNDSGIVIGQMSKPNFITGKVNNNESTESNGHPTHQRTPSGSSMFRKNKTVTKKFGFGMTPMHQHQHQSQSSLPAMNQGTPPPTQQPHNPLGLKNKSRSFSNFFAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S065</accession>
    <name>A0A1E5S065_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Altered inheritance of mitochondria protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g226</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the BolA/IbaG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5S065"/>
    <dbReference type="FunCoup" id="A0A1E5S065">
        <property type="interactions" value="95"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S065"/>
    <dbReference type="InParanoid" id="A0A1E5S065"/>
    <dbReference type="OrthoDB" id="203381at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.90">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052275">
        <property type="entry name" value="Mt_Fe-S_assembly_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46188">
        <property type="entry name" value="BOLA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46188:SF1">
        <property type="entry name" value="BOLA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ92395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="112" mass="13108" checksum="3800C53F874AE682" modified="2017-01-18" version="1">MFLKTVPLRLRPYCPRVPHFPQLTTLRHNSTFPLQTDEEKNIYNKLKQNFQNPKELLVQDISGGCGSMYKIIIQSAEFNTLTMIKQHKLVNSILKDDIKKWHGLQLTTKKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S073</accession>
    <name>A0A1E5S073_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tag1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g155</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5S073"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055012">
        <property type="entry name" value="Tag1_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20775">
        <property type="entry name" value="Tag1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Tag1 N-terminal" evidence="3">
        <location>
            <begin position="145"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ92405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="350" mass="40339" checksum="55150DEF0E3B78B0" modified="2017-01-18" version="1">MGEPDSTTPLLSANTSSNKAQQHQPQHTETGLQEYLSEENTYTNNNNNNNNYAAISTPEISDDQYREMEESFASSKKRSRAYTYLRAVLIFFVALCFLLPLGCMIFSVPLMRAVDNKYGNNYLQSFVENSTNIDVQDIDWHILNSKHIQLNITADLSFDYLASVNDASFASTVNSVALLPRDKTLRKLMRFSMNHAVKNMCLMVDNIEVVGEVHKDDEETSHADNVKWSLSNLKPGSQDELLCFSVMENVTTRVYIPILLELNWTNLYKIIKXINKEKDRDALQRNVEIFSVLDLQVYKEXFLGQKLPILKNLHIDKFDVTKYILDTGYYTELAEPYLRPSSFENWEERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R094</accession>
    <name>A0A1E5R094_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">U3 small nucleolar RNA-associated protein 20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g4486</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R094">
        <property type="interactions" value="1071"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R094"/>
    <dbReference type="InParanoid" id="A0A1E5R094"/>
    <dbReference type="OrthoDB" id="360653at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052575">
        <property type="entry name" value="SSU_processome_comp_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057525">
        <property type="entry name" value="UTP20_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046523">
        <property type="entry name" value="UTP20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011430">
        <property type="entry name" value="UTP20_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17695">
        <property type="entry name" value="SMALL SUBUNIT PROCESSOME COMPONENT 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17695:SF11">
        <property type="entry name" value="SMALL SUBUNIT PROCESSOME COMPONENT 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20416">
        <property type="entry name" value="UTP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23099">
        <property type="entry name" value="UTP20_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07539">
        <property type="entry name" value="UTP20_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 20 N-terminal" evidence="3">
        <location>
            <begin position="830"/>
            <end position="1415"/>
        </location>
    </feature>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 20" evidence="4">
        <location>
            <begin position="1607"/>
            <end position="1824"/>
        </location>
    </feature>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 20 C-terminal" evidence="5">
        <location>
            <begin position="2252"/>
            <end position="2495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2480"/>
            <end position="2506"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2408"/>
            <end position="2435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="2480"/>
            <end position="2492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ80322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="2506" mass="287841" checksum="C93FF99536B7AE9F" modified="2017-01-18" version="1">MVKQRIGSTKGANKFRYTSFRNKIDDLKIEPAKSLIKRHHDYVETSHFLSTFEHWKDINRSXNFSNFAEENESKIQTLAQILYHQQAIFSSLKAHIDLHDDKSLEPLCELLAQFVHDLGPDFMVFYEDTLKCLLSMLENAVSVENSSVFEWGFNCLAYIFKYLSRVLAQDLNRTFELLFPLLSHQKEYLSRFSAEALSFLVRKSKATSLQHFIESSVAKLDETHDVHFYDGLETLFEESLKTTSESLHSKTNVIIGALLPQVINSNGSDSISLFCDILMDILRHASSENVAEVYHFILEKLSESVASKKCDTTDKIAKVLATLGFAESGRKVPSWENFVTIVQRLMDNASPENKPRSETFAFLFAVLLRNADVSVLTQFHKKMFEFYLAEYTDSFLEFFTTCLNMASERVFVFNGSRFLQKYIRDXWATSSGKLSLFLFKLEENETXKEKLNITLPEEFISKTFSDMISANEDYYKILTCLSILSYRXNDDQNIDYFSSLKGMSKCLNELNDFQKDVFGVFLGLSLSNYNVDDEGVYDILSMVKERMSALRNSLFFVKGLRTFLQCLKKNDLFAAKLVQLVKDDDYLLIKELTNNLLLPNSKLRYETLYLLYDLMGMVNYDVPDVLKTCAILEEIPQTLEHARDFTARIRSMGSEFAKTTSNTIYNMIFFKHMFGLLNVRFSPIWDGVYEILPNVYEKDQQLVWDLSLQFLTVLDKNFVLQYPEEPMVELTRDEWWSVSVVRLDDAINACNKVFDGYFFEEGSLLDIMKERRGNLEYPQLIRNQTLKLLLSISSLAERHSRDLVPFFFNSKENEEIFGEQLEDKSNTAASWNDADRNLLLKLLGKFKNIRSVYHSDEIYERLLTLLSSRNSEVQKLALSAIFAYKDKTINKYKENLNSLLDDTLFKDEISRLMASDETKIIESQDEHVLIPVILRILFGRAQTPSTSGIKKSRKNAVITVLPNFDEKYVTQFLQLGSERLNFSYYFNDYIVAKEEVTLLTLRRVVGFVTIINSLFSVLGVKFPKSLESTIAPLIYSSAVSSFVLQEKMSENNIDKMAINARQQIMKAFYQLFEYYSETLPWDLYIDDLFKVVVNPRLKAFEEENLQQPSAMMKMITFWSSHKTLYKFLYYNNNAAARALIRTLSNDKAKESVVAIILSFVNNLVTGATEESSYAELVAICVSGSLLKLPVLFSEAMSPEVSTAAVNLLLNITNAGFIQDNDTRRLLVDSLSVALEKHAKSIPIKDVAKILSVLVTVVAEYDCSWSDIEPLYKQLSVLFRYYVDKTVRVTLANVFVSIAQRFPDFEKVANLLSDLNSYGNDRMQGYDFKRRLPAFKEIVENDFKTMNDLEWMPILYSCLFFINDFDELAIRTSATSVINSYTDYLNLQTDEVRSQKAVQLMKEIVLPNLKLGLRKKKDDIKNEYISVLAYIVTNSEVYTDLNDMKVLLMKGDQEADFFFNINHIQIHRRQRAVKRLCELAPQLHENSISHYLIPAIENWVFCEDEKYRNLCNEVITALGKLSNFVSWNQYKALIRRYKSMLKMDSPHLKEIVLVIVNVSVSIKNSLLFLSDANCGLPSIRKFPSKLDEPKLFIANELYSDLTKFLNKRDDETIVARIPLSEATVNLILGLPQDTVVSYLPGTLTSVCQVLRSRSEELRDAVRKNLSKLAVILGAAYLSFIIKELKGALTRGSQIHVLSFSIHHVLVSLNDHLVQGDLDSSADMIAGVIMEDTFGAAGQEKDAEGYTSKMKEVKHNKSYDTAEILSNSISLEKFGSLLHPVKALLLEKLPLKVQNKLDELLRRYVLGLSKSPESAKFEVLALCHEIYQQSLGPEAYKTFRKKRTLDEKEEFFTVDLNSKFTHVETESSLFVHTLQRLSFDLLRTSLSKNANLMDAQYLEGFIPIFKKSLNSDDEGVLISTLRILTMIVKINFSEDNEPIFKNCARKVFNIIKDSPSTSSELCQLGLKFLSVLIRHKNVEIKNSSLSYILTRILPDFNEPHKQGLAFNFLKSLITKHVVLPELYDVVDVVSEIMIANHTKEIRDVARSVFFQFMMEYDHSKGRLEKKFKFLIDNLQYSSPEGRISVMETISLVINKSSTELLSKLSSSLFVGLANVAVNDSFPKCREMASQLLHDLLTKVGEEGTSTMEKYIIAWLKQTSNNQYLSLGLRIYKIYLEVFGLNKEHNQELHELAIMRVQYIIENTDLGSEIPWELIYLALQLFGSYSNKAGGETFKSSFHAAWKNVFNCLLYPHSWVRLAATRLVSLMVEQKDNFESFPSDYDIQTIAFRVFRQLGAPSISEKLADASVKILVQIIMSWKEKDQQYIAPKGQEHEEEDDEENGDGQELKYISSIDFAVSKAAHILRSEKLVLSESFVGKKAMIQLLALMIQILDSAIVEKESEKIIMSLFMYLENERSIDEQEEELQDLAKECLQFLENKLTVSQFTAAYSAVKQEVYVRRQERRAKRAVLAVRAPDVAANRKFKKHARSREKRKHQKDENGFYKKKRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0D9</accession>
    <name>A0A1E5R0D9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cyclin-dependent kinase</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Serine/threonine-protein kinase PHO85</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g4560</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0D9"/>
    <dbReference type="FunCoup" id="A0A1E5R0D9">
        <property type="interactions" value="486"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0D9"/>
    <dbReference type="InParanoid" id="A0A1E5R0D9"/>
    <dbReference type="OrthoDB" id="1732493at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009891">
        <property type="term" value="P:positive regulation of biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000611">
        <property type="entry name" value="CMGC family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000062">
        <property type="entry name" value="PHO system negative regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF46">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="47"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ80347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="357" mass="40124" checksum="C0B7F9C20D94A63F" modified="2017-01-18" version="1">MSQQDTFADQTGGVPPASADYNQPSSSSSPRSAHTSNAGSGQPFSKFKQLEKIGKGTYATVYKGLNTKTGQYVALKEVQLDSEEGTPSTAIREISLMKELKHENIVRLYDVIHTESKLTLVFEFMDNDLKQYMDNVYRQRQKTLGTTENVPKGLDLALVKFFAYQLLNGVNFCHENRILHRDLKPQNLLINKNLQLKLGDFGLARAFGIPVATFSSEVVTLWYRAPDVLLGSRNYFTSIDMWSCGCIIAEMITGKPLFPGNKDEHQLKLIFEALGTPNVLGDDTWCRNLPKYTPEIQSLGIYPKDMKDVLGITANQNVDPQLLDLLSCLLQLNPDLRISSQQALEHPWFQDLRAQIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0H2</accession>
    <name>A0A1E5R0H2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trehalase</fullName>
            <ecNumber evidence="5">3.2.1.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Alpha-trehalose glucohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4493</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>alpha,alpha-trehalose + H2O = alpha-D-glucose + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:32675"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:17925"/>
            <dbReference type="EC" id="3.2.1.28"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the glycosyl hydrolase 37 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.28" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0H2">
        <property type="interactions" value="337"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0H2"/>
    <dbReference type="InParanoid" id="A0A1E5R0H2"/>
    <dbReference type="OrthoDB" id="3542292at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004555">
        <property type="term" value="F:alpha,alpha-trehalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005993">
        <property type="term" value="P:trehalose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000026">
        <property type="entry name" value="Trehalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001661">
        <property type="entry name" value="Glyco_hydro_37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018232">
        <property type="entry name" value="Glyco_hydro_37_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011120">
        <property type="entry name" value="Trehalase_Ca-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23403:SF6">
        <property type="entry name" value="CYTOSOLIC NEUTRAL TREHALASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23403">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01204">
        <property type="entry name" value="Trehalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07492">
        <property type="entry name" value="Trehalase_Ca-bi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00744">
        <property type="entry name" value="GLHYDRLASE37"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00927">
        <property type="entry name" value="TREHALASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00928">
        <property type="entry name" value="TREHALASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Neutral trehalase Ca2+ binding" evidence="7">
        <location>
            <begin position="104"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="46"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="747" mass="86082" checksum="456194D8E16C38FF" modified="2017-01-18" version="1">MEKYALNNNSNNAPNPAAAHKKAAIKGALVNPYSDAETYYSAPTQRSSSPTSRLRSFSVSQNMSNDKTNWIKTYQLQRRGSEDVSSFAEKGKRLFFIEXVNKTLEDLLKREDTDSNYQITIEDKGSKVLKLGTASSEGYKFVNIRGTYMLSNLLQELTLAKRYHKSQIFLDESRLNESPVNRLQRLISTAFWKSLTRRIDGESILKLARDTKFEDESTVRIYIPYNCPDQYDFFTKAIGQNPLVKLEVEYLPKDITPEYVQSINSKPGLLTLAMDKVQDPVTGKDKLVGWPYVVPGGRFNEFYGWDSYFITLGLVESQENVDLCRGMVENFVFEIDHYGKILNANRSYYLCRSQPPFLTDMALQVFKKLGGSGNQEAVDFLKRSFNSAIKEYKNVWHCKPRLHESTGLSCYHPDGLGIPPETEKDHFDSVLQTYADKYNVSIDDFKEKYNSREIKDASLDDYFLHDRAIRESGHDTTNRFEGHCCELATVDLNSLLYKYEVDIAHVIEKYFDDKHIDFEGVETTSKHWADLAARRKKVMNKLLWDEEKGMYFDYNIKSRKRTTFESATTFWPLWAGLATQDQADKMVKNALPLFELFGGLVSTTERSRGVVSKSRPQRQWDYPYGWAPHQILAWIGLEKYGYPGEVARLAYRWLYIITKVFVDYNGAVVEKYDVTSEIDPHRVSAEYGNQGIDFKGVAEEGFGWVNTSYVMGLKYMTRYSKRALNNCVSPKIFFERLPPKEKKLYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0K1</accession>
    <name>A0A1E5R0K1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytochrome c oxidase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4417</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome c oxidase subunit 6B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0K1"/>
    <dbReference type="FunCoup" id="A0A1E5R0K1">
        <property type="interactions" value="438"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0K1"/>
    <dbReference type="InParanoid" id="A0A1E5R0K1"/>
    <dbReference type="OrthoDB" id="1107506at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00926">
        <property type="entry name" value="Cyt_c_Oxidase_VIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.140:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 6B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.140">
        <property type="entry name" value="Cytochrome c oxidase, subunit VIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048280">
        <property type="entry name" value="COX6B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036549">
        <property type="entry name" value="CX6/COA6-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003213">
        <property type="entry name" value="Cyt_c_oxidase_su6B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11387">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02297">
        <property type="entry name" value="COX6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000278">
        <property type="entry name" value="Cyt_c_oxidase_6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47694">
        <property type="entry name" value="Cytochrome c oxidase subunit h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="28"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000278-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="83" mass="9824" checksum="E36E36A76F71EC7B" modified="2017-01-18" version="1">MAEKLDHIELKTPGFDPRFPNQNQTKHCWQSYVDYYKCINVKGEDFAPCKTFFRTFTALCPLDWVSKWDEQRENGTFTGDIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0K5</accession>
    <name>A0A1E5R0K5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alcohol dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g4617</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10736"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0K5">
        <property type="interactions" value="1046"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0K5"/>
    <dbReference type="InParanoid" id="A0A1E5R0K5"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000947">
        <property type="term" value="P:amino acid catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08297">
        <property type="entry name" value="CAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000039">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000002">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF3">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="10">
        <location>
            <begin position="18"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ80431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="352" mass="37599" checksum="112EAC374F7E5778" modified="2017-01-18" version="1">MSIELPEKQKGVIFYKNGGDLXYTDIDVPKPKSNEILINVKYSGVCHTDLHAWKGDWPLAVKLDPYLVGGHEGAGVVVGIGSEVKGWEIGDYAGIKWLNGSCMNCEECELGXESNCPQADLSGYTHNGSFQQYATADAVQAAKIPKNADLAEVAPVLCAGITVYKAIKSANLKPGQWCAVSGAAGGLGSLAVQYAKAMGLRVLGIDGGEGKEKLFYDLGGEKFIDFTKEKDIPKTIVEATQGGAHGVINVSVSEKAIEQSTQYVRSNGTVVLVGLPAGAVCKSSVFDQVVKSYKIVGSYVGNRADSREAIDFFSRGLVKSPIKIVGLSELPRIYEEMEQGKILGRYVVDTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0L0</accession>
    <name>A0A1E5R0L0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4448</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R0L0"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="398"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="398"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="654" mass="74691" checksum="A8DB1A88F7875DE2" modified="2017-01-18" version="1">MLVKVAFNSLWCLKKKIKATELEKKEKELKAIAMTLSIYDKATFLPDTLKEDAQKYCSSEKNNFSLNTEESLLSDKFASMFFDNPQSKLKQSPGTKDQSRNSPFEKHDNKFCNQFTSKCSNVDWNTDVLDYFEDIKVQKSIHNKGTASCSNFYLTHKSYSSSTKNAKVWGCDCNNSQXFXNSDVQSSFSLYRNESRSSVQLLETESLLKKXKSNLDDAIGYESVRATYTKKKDRSHTISTPQTKLGKRTRVCKKIAKIATKITTFSNSPKPLLAEIGKLPPEHSYTKNNQLQTLFKMEYSSELSVEPPNRQDETDXLQFSSYSQXGKLVARLNQAETSIFYPRQLSYFEHPYLVTTESNTRSFIRVETEAERKKIIAQHDRDQLNCIIKHNSSLKSTSAQTLTPTSSLSQSTDGIHGVHPSRTRQYFSNLKHALAGKLTFRKESSAETSAETSVEXLGPSNAENMINITEKKEHSDANSHLKIDQWFSSNKPSKNNPTQFRKNASFVHPSVLRLFTSLGFFEFENKKXLPGKIADESELLGNTTLGSFPRLKHSVNTLVELSEQVLSQKICNQTETTMWSMFFWKLLLHFPQASYIYCKHCGDAIAANWDCIEFRKQLNDHNKQVCKQCQLAESQQKWAGKKEKKILFLSKCLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0N4</accession>
    <name>A0A1E5R0N4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4572</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R0N4"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014804">
        <property type="entry name" value="Pet20-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08692">
        <property type="entry name" value="Pet20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184549" evidence="4">
        <location>
            <begin position="18"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ80461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="349" mass="38256" checksum="12E3706AED42632D" modified="2017-01-18" version="1">MCLTILLLLSASSSSSSSSSSSSGDHHHPAHTSTKYKYKYTRFPRILSVEPVYESTSYLTPIKHSNPLTSVNALKLREDTNKTNDDYYDSLYSTSDSGSSSTASATKANTLVNGTKTSVNYLKSEKRKNSGLAYDMFFNAYRPLNNVFANGSNXXAISKSKDQXLFQQLYKSVNTPINDHAVVKTPKSAIPKDGKYSEQFETVLKGQNDQGGIKTIGLQSHYKYSSRVPKELMISINKKMIPMVGVVSAMFMECDKSWINTITTSSMGRASDYCSCMNDSAKKNGKAVSLVNHSFEPYSLERETLDFNTASTNKGSACGVSDCKLSSRKTNTKATGDANNISNIIGKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0U0</accession>
    <name>A0A1E5R0U0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">AP-2 complex subunit mu</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4521</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0U0"/>
    <dbReference type="FunCoup" id="A0A1E5R0U0">
        <property type="interactions" value="175"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0U0"/>
    <dbReference type="InParanoid" id="A0A1E5R0U0"/>
    <dbReference type="OrthoDB" id="3973245at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005992">
        <property type="entry name" value="Clathrin_mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="7">
        <location>
            <begin position="214"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="496" mass="55414" checksum="0AADECF426490278" modified="2017-01-18" version="1">MISSLFIYTQRGDLIVTKALKDSIRKSNSLAEVFRVQVIIGGGNGAASNFSDQGVGNGTGSDNGDTRSPILTLGSTTFHHIYKNNMWIVAVSRNNVDSGLVWEYLCKLHGIMVEMYAINNDELLRDNFWTVYEILDHTLNVNGVPFDTDMHSLCERIVSKKPADLSKLTLAHNKEGHGLAGKTGSISSYNPATAVTKDISNIPWRKNQDIHYKKNEIYINVLENVNLLISKTGEILKNNIDGKINVDARLSGIPLVSFELTSNSVDQSSMKFHECVKLEKRYRDGEKNWDDYSKQEGANLITFVPPDGPFELLAYHSVGISSIPFSIVPTITQTVNNKTSNIEMHYSIKIKSLFANKIATNVVMRIPLTDSTADCSMELSGGKCKMVSANNHKKFVEWTFAKFKGSTEHFITLTAKTTTASPVTTQFDRKDTSNVSAKQQPISLAFEISMYNGSGLTIKNDRVRNQLISKNGTDHQYKVHKWIKYDTRAGNYEVRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0X5</accession>
    <name>A0A1E5R0X5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative phosphosugar isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4563</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0X5"/>
    <dbReference type="InParanoid" id="A0A1E5R0X5"/>
    <dbReference type="OrthoDB" id="1872003at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38418">
        <property type="entry name" value="SUGAR ISOMERASE, KPSF/GUTQ (AFU_ORTHOLOGUE AFUA_6G08860)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38418:SF2">
        <property type="entry name" value="SUGAR ISOMERASE, KPSF_GUTQ (AFU_ORTHOLOGUE AFUA_6G08860)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SIS" evidence="1">
        <location>
            <begin position="47"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="357" mass="39893" checksum="2F3A81CF31557968" modified="2017-01-18" version="1">MTTQPQEPRPALKLLHATKRGLSELLLALEGRDASRSESFDSCLDIMQQCIERGGKIIMVGCGKSYKVCDKITCMLNSLSIQSLKLHPVDALHGDIGNYRADKDCLVFVSASGETKEINSLIEFLQYRHSGMSLXMIGVTQYKDSLMAKLLMQTCDSGKCVSILEVLCGDTTTCKEACVFPGLKTPTISLTLTLQLLDAMVIELAYNLYGHNWDRVIKMFEDNHPNGSIGMNNGIHSRSGSPXECNSPVQSEARKQEISXKSIFSTQTFHCTKKKLEDLDDLEVLNVFLTNYFVTVLGSTEKYESIGQDTIYVECSSALDAYRDVCICQNKLTSPKNKKQSFKEYFIKEFLENDVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0Y0</accession>
    <name>A0A1E5R0Y0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Protein MAM3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g4476</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0Y0"/>
    <dbReference type="FunCoup" id="A0A1E5R0Y0">
        <property type="interactions" value="87"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0Y0"/>
    <dbReference type="InParanoid" id="A0A1E5R0Y0"/>
    <dbReference type="OrthoDB" id="5353557at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010960">
        <property type="term" value="P:magnesium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000006">
        <property type="entry name" value="DUF21 and CBS domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045095">
        <property type="entry name" value="ACDP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064">
        <property type="entry name" value="METAL TRANSPORTER CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064:SF97">
        <property type="entry name" value="METAL TRANSPORTER CNNM-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5 7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="116"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="11">
        <location>
            <begin position="112"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="10">
        <location>
            <begin position="380"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="453"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="529"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="680"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="822"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="453"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="504"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="531"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="680"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="831"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="866"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="892"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="907"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="934"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="952"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="977"/>
            <end position="986"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ80540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1005" mass="109735" checksum="A87CB10452B1483D" modified="2017-01-18" version="1">MKIPNNNTIIASNNSNGNTSANNNVQILTSGGDTEDDAYQERTSCVATLQNNTPVEMCKTSPKTSMWVFYFALLPALCKAFPIFGHNTMNNMQTISNVAAFKATETASPHTGEKNFVAYLLVAMLLIILGGIFAGLTLGIMGQDEVYLKVISVSGTSRERKASLKILELLNRGKHWVLVTLLLSNVITNETLPIVLDRIAGGGWQAVLGSTVSIVIFGEIIPQSVCVRYGLEIGAFFQPFILVLMYIMYPVVKPISIILDMALGESHGTVYKKNGLKTLVTLHNSESMLGVEEPLTGDEVTIISAVLDLKDKKVQEIMTPLENVFTLSCDTILNEERVEEILNSGFSRIPIHVPNKPLDFIGMLLVRILISYDPEDCWKISDFPLATLPETGPTTSCLNILNYFQEGKSHMCIVSENPGFSHGAIGVLTLEDVIEELIGEEIVDESDVLRNSPLATMNNSDQISNGQHTNSGSDGNLLLAQTNSETRKNSVSSFKKSLSTVKTRLNKNKNLPKRQKSLKRQSIDHTNAMHASDHENFDHEGNDYNPQTTKKTTTIITTEVPDNDDNNHNNNTADKIVQIVSPEAKENTPLLTVQHDNSAVSPIQTTEPGSPPSDFCTASHLPKTTAMGPQPLNRASDPLKVNKNFVTIKQLPKEFAQRQTHNLQHLNASSIYLDSHTADQGTHHTYSTQKQSNSMSPLITLSLPPNSPHPQPETLQETQPSVSVVLPHVDPVTYNNPQDFHNEFVASTTYNLNSNLRNNHSSSPSYIGSSSTSPMVINPQSHFQKRSESHGGIVESVITVEGVKKTIIEQAVVGSLGRLVGEKKDTSTHNKDKKSKSKSKSKSKSKSQSKKGKNKKNKKKNTSGSESANVSTVISNEENRRHYPNSNRSGDSDVDSNESSDSDDTENSNTSNSATSEEASTSRNGEDEGTVRKVLGKIRRLSHVSKEGKNDFGNSNKENSNSGTTSPVVGANTNYLSSSANATSKSSSRRSSSKANPPEESFQMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0Y4</accession>
    <name>A0A1E5R0Y4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA repair protein RAD5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AWRI3579_g4416</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0Y4"/>
    <dbReference type="FunCoup" id="A0A1E5R0Y4">
        <property type="interactions" value="1037"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0Y4"/>
    <dbReference type="InParanoid" id="A0A1E5R0Y4"/>
    <dbReference type="OrthoDB" id="2801544at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014905">
        <property type="entry name" value="HIRAN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF22">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08797">
        <property type="entry name" value="HIRAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14634">
        <property type="entry name" value="zf-RING_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00910">
        <property type="entry name" value="HIRAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="465"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="863"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="958"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="274"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="304"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="317"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OEJ80542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1126" mass="129220" checksum="4C35CB81EE736778" modified="2017-01-18" version="1">MSKPFFNSALDDENSAPAKLDTINDTENGDIASQETSIFDLKPFDEGEVSQKTRKAEKSLERTFAKKRQLDFYNRHTWHKFIGSFIVEGTATRPTTKPLIYGSKLSFLVTTLESNSKVKIKRSMGSLIKFWDPATRREVGRLPEKHASIIYPFLYNSDTEDPTSKKEVSFKATIVYAEPKRISVGDSFLVQIDCFLNSSLFELSSTTRPSIEASQLQNTTDLGHSLNGKKSSNILFGRNTDLDQHEEKLHRRRKALMTLFDELNLKPVPPNDDISIVSDTQNESDADQTLDVSTASAQKKEQENIEVIDLDDEDENDAIKEEHQGSRYKKYYDERPNAESSPELSVNQLQNIYKITQSMSSLMDLPETSPSGGFQFTLRKYQAQGLSWMLKREHAFDKIHDTEFINANSNKMNPLWKKFKFPNDSAWSLNKTPDTTKETAESEFFNEPSHFYANLYSGEFSLKVPTVKLMTKGGILADEMGLGKTISTLSLISTVSKDPDFQPYDIVKISEDNMKPYAYQTTLVVVPMSLLFQWKSEFENAMAGSSKYCEIFYGDQSQKKNLIQHLCSNKSTPTVLLTTYGVVQSEFSKYAKVKQGNFGLFSVKFFRLVIDEGHSIRNKQTKTAKSVFELKSSRKWVLTGTPIINKLDDLYALVRFLDLSPFSEPGFWRTSVSEPFENKKYKAALDVVSTILEPVLLRRTKNMQDADGAPLVELPKKNVKIQYIELSPLERRLYQSFLQVAEKNVRDSLKKGELLKNYSSILVHILRLRQLCCDLHLLMKAKKNKEAVQQSKFDDIIMEEDSEENQAENVAEAEHLDLAQLLKMSILRDRFFDQKDLAYLQNKDEFFGNLIGAEASSLRKMECQICLSSPIEPLESIVFTECHHIFCSDCMDRYLSHKETKNESAVCPICRTPELAPARLLSPIMDNTNKTIASFRIESPPVLEDFVQSTKMFHLTNDLYELYDDNPSERVIIFSQFSTFLDVIQNELEKKEHDSRFFVSKFDGRLNLKERQLVLQKFDQDSENNQLKVLLISLKAGGVGLNLTKANKAFLMDIWWNAGIEDQAIDRIHRIGQERDVFVKRYIIKNSVEEKMLKIQERKRMLGEIVEGDAEERRQRRIEEIKSLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1B3</accession>
    <name>A0A1E5R1B3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">GTP-binding protein YPT31/YPT8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g4327</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Golgi apparatus membrane</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1B3"/>
    <dbReference type="FunCoup" id="A0A1E5R1B3">
        <property type="interactions" value="1010"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1B3"/>
    <dbReference type="InParanoid" id="A0A1E5R1B3"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034498">
        <property type="term" value="P:early endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000067">
        <property type="entry name" value="ras-related protein RABA1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="204"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="205"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="244" mass="26782" checksum="72C63F8E826CA77A" modified="2017-01-18" version="1">MSTSGTAADSGNNGATKDNQDYGFEYDILFKLVLIGDSGVGKSNLLTRFTANEFNIDSKSTIGVEFANRIVQIEGKKIKAQIWDTAGQERYRAITSAYYRGAMGALIVYDITKSKSYENCSDWLKELKENADSNIVIGLIGNKSDLAHNRSVPTEEARNFAKENQLLFTETSALENDNVEKAFEELITAIYNIYKKHQANIDIGQDSQSNSNTNGAGINPNGKSLNTVSLKDQKTKKSSSSNCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1J6</accession>
    <name>A0A1E5R1J6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PHO85 cyclin CLG1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4333</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1J6"/>
    <dbReference type="FunCoup" id="A0A1E5R1J6">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1J6"/>
    <dbReference type="InParanoid" id="A0A1E5R1J6"/>
    <dbReference type="OrthoDB" id="244495at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20557">
        <property type="entry name" value="CYCLIN_ScPCL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF27">
        <property type="entry name" value="PHO85 CYCLIN CLG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin N-terminal" evidence="2">
        <location>
            <begin position="120"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="250"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="456" mass="52001" checksum="153CB8FC62CB2E54" modified="2017-01-18" version="1">MSLVYQTQNQAQAHASAPYGYHNNVNNQMFPGAQYYPQNASYMPQGYAPQQFQPQPQQPQDQPSAGGVCETVDYDFKMMTEFVIQNAYLTFDKEIIADFDDKTLENSDVVEIFTNGVTFVLKSIRLPESTIYLALNYLHKSVNKLKKQHSSTESKSSIFADVSENVIYQYLVIALVLANKFNDDKTFTNKSWSEATGMDLQTINRMERELLNLFNWNLNDVIFETKLYSEYAKSFEIYSNAMSCKREASKQSSVPSAAPSQPLNLYTQQQQQQQQPSYDYVESSPLLNYNYGANPSVQAPQYSTHGHSDLYHSAATPGSSNGPETPITLPSMLNNSSNNYFFSSPMAPESQSFMVQPQQFQPQPAKPYNDFSSPSNYYNNQYSQPSANMGVSSNYYAHPQNYMSGPMPVDMQLQNQMQMQMQMQPHLQMQMPLHNTQQQFHPQQQGFYQHPLLSFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1J8</accession>
    <name>A0A1E5R1J8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein SSD1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3962</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNR ribonuclease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1J8">
        <property type="interactions" value="257"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1J8"/>
    <dbReference type="InParanoid" id="A0A1E5R1J8"/>
    <dbReference type="OrthoDB" id="372421at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.690:FF:000001">
        <property type="entry name" value="Cell wall biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.700:FF:000002">
        <property type="entry name" value="Cell wall biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041505">
        <property type="entry name" value="Dis3_CSD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041093">
        <property type="entry name" value="Dis3l2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050180">
        <property type="entry name" value="RNR_Ribonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355:SF9">
        <property type="entry name" value="DIS3-LIKE EXONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17877">
        <property type="entry name" value="Dis3l2_C_term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17849">
        <property type="entry name" value="OB_Dis3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNB" evidence="3">
        <location>
            <begin position="762"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="52"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="347"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="461"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="610"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="67"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="87"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="179"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="191"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="204"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="290"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="311"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="351"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="495"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="514"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="531"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="547"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="614"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1342" mass="150288" checksum="8681495CD67EB62D" modified="2017-01-18" version="1">MSSHHNSNKGSFNTNQQFVTPMGNNKNNPQKIHVAHRRSPSELTNLMIEQLNIGNENRSNESRSNEGRSNYHNSQNPSPFLPPPPLQGNANNNNNNNNNNYYHYSGHSNSNSNAGSRGHSRNNSYGNAQNSNGGGGSDTXYHKRKSDSQSSIVLQPPQSSSSSSAQQGHSRRHSLGLNEAKKAAAEEQMKRQGQMSGHNTPPHSSTSSGLSSGVTTSSTLSQPPVVQAHGNGLNHSRNNSLNAFTSPNKSFQFPAKPPSFGNSNSNGNDNDENSGQDYHYAYSPRKFQQSHSRNNSGYNSGSTGGSGGGYANNNNNNNNNNQHYSHKNQNSHSGQQSLLLEPLQTHHHHYNNSNSSNFHKHSMSNSNSNSNSNANPQSQRKSLFAPYLPQANIPQLLQEGKLVLGTLRVNKKNRSDAWVSTDGVLDADIFICGSKDRNRALENDLVAVELLCVDDVWGSKKEKEEKKRRKDQGSVQQDLLNSGEDYHNDATVLSPRKDSTKRSSFIDEPVIPSAGNSNLANFNNDNNNNAKTHENDFKNDSDQQQGLKRRGSLKQRPTQKKNDDVEVEGQSLLLVEEEEINDDFKPLYAGHIVAVLDRIPGQLFSGTLGLLRPSQSSSSNSSENNNNKDNNNNNNNNNNEHVRKPKIVWFKPTDKKVPLVAIPTEQAPEDFVTNSENYANKLFIASIKRWPITSLHPFGTLVEQLGEINESATEVEAILKNNNFESNEYLDPQNRSREKPIFKPYPFQSDTTQRPVYQSPNIHTIKQSLNGPHDMALQVEHFAPGGSNAGAEIVRFSVHVVDVTEHIENGSSLDRRARKRSTGVYLPQKTVYLLPQKVNSELSLTKGSKATTLSVHYDIDLSNGKILGQKIEESFIVPDGESIGEEELEXAPNAKTAAPTAHFIESIQKIASLFNQLRMNNSKNSITPRLSLLETLDDEKIKISDLNYYFNENGYIMKEIHFKVNNTVAEFNYSKLGDQAFLRRHAEPLSTKFQYFLHRISEFNQFDTVLNLGNPELCLKSISKIEDNHLRKCIEILFYKTLNRSKYYIAGKVDPDQFGHYLLNYPIYTHFTCPFKRYADHVVHKQLKAVLKNQVDSFNAGNDIDSLKITAEYCNFKKDCAFAAQEQSIHLLLCKTINDMCKNSNNMDQVLTLGLVIQVYESSFDVYLPEFGIEKRVHGDQLPVTKAEFDNINKVLELYWNPSIDSATFIPNDEKKPNSYRSSIKNNFKSSSHNIAKTELRKIPELSQELQKTFQDWHLEPPTLSLLSKLTTKSSSLPETSGSASSPLNVFKNYTTTRIEGDLEKSHVQEIRELDYVPILVRAEIGMALPCLSVRALNPFLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1M5</accession>
    <name>A0A1E5R1M5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Enhancer of mRNA-decapping protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R1M5"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="439"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="145"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="502" mass="55929" checksum="8ADE70E509C6AB22" modified="2017-01-18" version="1">MYFNSSRLLQSNSRNKTPAVKQKQTYPQIQQPSQQQQQQQQQQQQQQQRKKNNTGNANNNNNNNKKKNHRPTSSCSSLHKKNAKKSTLNDDHSDTKVQDSLEHRKSSGSSTKHSKKDEYPQIQQLPNGERPNFGNSHKSSHKNSNKNNKNYQRRSSSNASGHNMNKTKGKNDEDANDNDVRFIIKNALNMHLNNSAVVSDEEPEQNPQGKMSRTSSHGSNGKSRRGSHRKSXNKVEADVNEKENEDDLXDFXARSAQNRSKAPISPVSPMGKPMMAPPMFLAPPVSSGSPLVQPINHMVAMPPLQQHQQPAPPPPPPPQLSTMFANGPQRGYPYQGTHAMGNTMNVPPLPMQMPIAMPLKQAPSSPHPTFAMNTNSMMLHPAPPQLPQPHFMNMNHHHQNLGNAQHPIMPSTTNQGNYHYYPPQHANRVVPREIYHQSYTAKSPAAPANENKKDSKRNERRNTDVDGDASSTSLNSIGSQYAGASFTINAPTITKLPKPSFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1R5</accession>
    <name>A0A1E5R1R5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">tRNA acetyltransferase TAN1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4358</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1R5">
        <property type="interactions" value="905"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1R5"/>
    <dbReference type="InParanoid" id="A0A1E5R1R5"/>
    <dbReference type="OrthoDB" id="367221at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11717">
        <property type="entry name" value="THUMP_THUMPD1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2300.10:FF:000001">
        <property type="entry name" value="THUMP domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2300.10">
        <property type="entry name" value="THUMP superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040183">
        <property type="entry name" value="THUMPD1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13452">
        <property type="entry name" value="THUMP DOMAIN CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13452:SF10">
        <property type="entry name" value="THUMP DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="THUMP" evidence="2">
        <location>
            <begin position="144"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="283" mass="32262" checksum="D60FECCB989334B4" modified="2017-01-18" version="1">MSKRVRSSNGNAAAAKPNKKQKYIGAVGIIEPGTSGIYATCTRGKERFAQQELMQLFQEKYQEMYAATEDGESGDXEVEGSDHEELDMEQQIAKELEDLKKQNVQIKTHKAKISPIDLQCECVTFYKTRKPVVPEKFVHELMKDFKNNELKRTRXIAKILPITNSCTATISELEKLCDKVLAAHFHTDEALKQPLKFAVDVTRRNFGTLERLDIIKTVAAKVGQNGENKHRVDLKNYDKLVIVECFKKNIGMSVVDKDYLTEFKKYNVQQIFESNLELDDNKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1W5</accession>
    <name>A0A1E5R1W5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alkyl transferase</fullName>
            <ecNumber evidence="4">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4495</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1W5"/>
    <dbReference type="FunCoup" id="A0A1E5R1W5">
        <property type="interactions" value="402"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1W5"/>
    <dbReference type="InParanoid" id="A0A1E5R1W5"/>
    <dbReference type="OrthoDB" id="4173905at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904423">
        <property type="term" value="C:dehydrodolichyl diphosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016094">
        <property type="term" value="P:polyprenol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1180.10:FF:000005">
        <property type="entry name" value="Alkyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291:SF43">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT DHDDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ80543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="296" mass="33635" checksum="6AF4A0644DE14109" modified="2017-01-18" version="1">MKDGLEAPNLEDPLAAGFLSNTSTWYNRMLKSTLSSIIKVSNHVPQHIGFIMDGNRRYAKQHKLKVKQGHDLGFFSMCSVLELCYECGVKSATVFAFSIENFKRSQSEVDALMELARKRILQIVQHGELCAKYGVKVRILGDRSLLDDDVLEELQKAEKITESNSRATLNICFPYTGRYDLYQSMHAIVQASGEGFLDYKNITAKTIDDYLYTRDDPPVDLLIRTSGVKRLSDFLVWQVQQRGVVLEFLDCLWPEFSVSDMWFILLKFTYNKTVNGEGSLPSNTQDTKPTLSQQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2J0</accession>
    <name>A0A1E5R2J0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">40S ribosomal protein S27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3901</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eS27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2J0"/>
    <dbReference type="FunCoup" id="A0A1E5R2J0">
        <property type="interactions" value="850"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2J0"/>
    <dbReference type="InParanoid" id="A0A1E5R2J0"/>
    <dbReference type="OrthoDB" id="5567124at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.100:FF:000001">
        <property type="entry name" value="40S ribosomal protein S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.100">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00371">
        <property type="entry name" value="Ribosomal_eS27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000592">
        <property type="entry name" value="Ribosomal_eS27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023407">
        <property type="entry name" value="Ribosomal_eS27_Zn-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11594">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01667">
        <property type="entry name" value="Ribosomal_S27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01168">
        <property type="entry name" value="RIBOSOMAL_S27E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ81098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="94" mass="10326" checksum="E6EC0FF6A236D68C" modified="2017-01-18" version="1">MGVLLKKIYKSCTQRVQDLLHPSAASEARKHKLKTLVQGPRSYFLDVKCPGCLNITTVFSHAQTAVTCESCSKVLCQPTGGKAKLTEGTSFRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2M8</accession>
    <name>A0A1E5R2M8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinase</fullName>
            <ecNumber evidence="4">2.7.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g4208</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the inositol phosphokinase (IPK) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.-.-" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2M8">
        <property type="interactions" value="211"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="2573163at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000824">
        <property type="term" value="F:inositol-1,4,5,6-tetrakisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008440">
        <property type="term" value="F:inositol-1,4,5-trisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.160">
        <property type="entry name" value="Inositol polyphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005522">
        <property type="entry name" value="IPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038286">
        <property type="entry name" value="IPK_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400:SF21">
        <property type="entry name" value="KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03770">
        <property type="entry name" value="IPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="56"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="194"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="572"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="665"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="23"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="90"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="216"/>
            <end position="227"/>
        </location>
    </feature>
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        <location>
            <begin position="228"/>
            <end position="243"/>
        </location>
    </feature>
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            <begin position="573"/>
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            <begin position="588"/>
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            <begin position="667"/>
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    </feature>
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            <begin position="727"/>
            <end position="746"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ81149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1103" mass="126171" checksum="414DEF520CCBAF88" modified="2017-01-18" version="1">MLKMKENSGHDTNGSKIVNKLWNNNNNNNNNTNNNNSHLSIKKIVDDIQHMSILNGHKNDADKSDISGSSGSSGSSSSNNSNNSRSGNRLTANINKQSVSQDTAMYGRKGSVSLRIFHNDEDNDQNKSVEMIDERGNICDERTSAYDKRTSAYDKRTSTPTLEHSNKFTKPFINESYSVKLKSPLYHVFEENQNESAIDDNDEDSDEIVPSKTKDQTSVPNLSSSSPTKTNIKANTSTNTNTTSVNKRLLSKDYLFNEPDESNVVKRVSSATYIPHTVPTVGKHDVKKSLLSNETLGITSDILDHNTFATITNDQDNDTKKHVRLVGEKLPIMSDSRMIKDKNPIIQETTRNIDTKNNKDKEHVDNNQQYEFPLAVELKPFKNKVGGHTEIFKFSKRAVCKTLVNNENVWYENLEHLYATKEANEKHXSSGENEKESHNILHYMPKYIGVLNVRQHFNTLNEYMEAVGELNHKNPQXKEGKGDTKXVTNEKQQXQQQQXLQCEKDSHRYMKKKPRCNSDTINEVVLDDNIHLFPSSFLNKINHVFSSXENQSTPTTSPVYGTSRSLMERRMSNQDISTSPWNKQIRRNSSSNNTNTLLSTSPSSTHSLNLTASNTEQIEMPKLQHSXSQSIIDDHFINTTMINTKLQEQVINEVFAPYKCTNLSHHNNNNNNNNNNDNXXNINNALLSAHPHDSPRSNSRSNSNTIGSMRNSSSPSLSARRRRKSDSTSFSSKKYAQTHNLKQTITPPLKSKQSDCDNEEAALMSDELTLLPEPSLTKQYSNENHNSKIEYQNNNEPLXVFETEESKTTALQNEQSLHSPQNDYTVVSQFILLEDLTRNLENPCVLDLKMGTRQYGVMANVRKQKSQSLKCLKTTSKKLGCRVCGLKIWDPKSKTYLKKDKYFGRRIRIGWQFSRILARFLYNKQTITSILVKIPKLVKNLDNLNETIKFLKGYRLYGASLLFMYDGSXELSHCKIYLIDFAQCLTKQDFVSKMDSMECPPKTSIQWEDKGFCKGLKSLKFYLKAIWNHLTNNHELIYDDKQLLQYLETNKEIFQKNWAWLDEFDVEDEAEFNDENSKLRKKWMKDELLFDVEPPFENSEESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4I4</accession>
    <name>A0A1E5R4I4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3635</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0A1E5R4I4"/>
    <dbReference type="InParanoid" id="A0A1E5R4I4"/>
    <dbReference type="OrthoDB" id="1259151at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="72"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="273" mass="30506" checksum="113F699A5CACF9DD" modified="2017-01-18" version="1">MPANHHPSLEVQGFQKLNEYEKIIHVYKNCSWNCYPIMSDNKVNKNYEGKLGQKGATVIITNLRLVLQSAQTNETNHSSETSLNSLSYSPPFPSSGTSTSTSTSTLTSTLTSTSTSTSTFTKSKSKSKSKNALDLTVEYPQVIYDKKVFQKGKKISAKMLPPELVIPWIGENYLLLKFQPGKEQQPYLNYIYPWVLEIYCKTDIFALKDCFSKVILESRTPTNGTLNNNNAVSTVDNHASESANVNGNHRQDSQCTPDCINADNFVPLPKYEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4K3</accession>
    <name>A0A1E5R4K3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3614</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4K3"/>
    <dbReference type="InParanoid" id="A0A1E5R4K3"/>
    <dbReference type="OrthoDB" id="5426978at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="159"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="274"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="479"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="539"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="603"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="576"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="57"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="131"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="318"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="329"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="547"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ81814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="625" mass="68227" checksum="B9E253B862453A08" modified="2017-01-18" version="1">MTSFRAKPNLISNLISTQQAARDTDTRQKGNTHTGQLKSDGHGEDLITTTRKASRYTTGTDTSNNNNTVPTTSISGYHSIVPQPDYRSISIGDIVLDESEMLSEEMQMHSVQSIDSIAAFPKNEVSKKVSKVQKKVSKKVNKKEKETQKQTQRKKSSRSCDYCRLKKVRCFFVNEKGVSDSNGKDGQKQCENCVKNGLECTFTRVQLKRGPGAGTSTSKAGASELSVDVAVDTGVSSSVDASTVRDIVPNVXISPNADVXASAYKVGKXAAKRQSKKXKLQKEAVPSTAFQQQEPYNQPRGNSIGSASGVALPPLSSFPDTPSETRGYTNSNNNNTTAISTTNNATNHTYSPSPSLTTPYAYSGGFSSLLPNGSTTLSGVNYNINMDTHRGSITQTQPSGPPPHTIESATTRFAHTPTSPPHQQFWKVPYNRRDSIVSLTSGISSSSERSSNENEHTNFFANGLVSGMNVEKLLLNLNSATPENNNPSRPHSTAPIPSTINNRDGVQPASATGDSPRQQQQMNNIFLANPMLQAFLEHQQSVHSGTQQPQQQQQQQQPGVRHSANTQEEEGVVGIIKVFINIIENMNKKMVKLENRIRRMESANPTFNQKQHPHSRPPTKNLPDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4K8</accession>
    <name>A0A1E5R4K8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Suppressor of lethality of KEX2 GAS1 double null mutant protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3559</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4K8"/>
    <dbReference type="InParanoid" id="A0A1E5R4K8"/>
    <dbReference type="OrthoDB" id="4097102at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051647">
        <property type="entry name" value="Mediator_comp_sub12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007:SF12">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="196"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="367"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ81810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="415" mass="46401" checksum="EA652290E447EBFB" modified="2017-01-18" version="1">MVSDSTSVAVGCAIGIPIGVGVLLAAVMWYRMQKRIEKEIEMDHDVIDKFDERDLDLQDIYDDSYKQAHQNGQGTIYNSKNIENYSEMEDMNLKKKRKTEKKDGNVVYTSTESGSLPNLAETENNKPNVSNDQEHGSRYIPAYKRKLYNSIKVLNKKTMPQRVPQDASTTEFGTESSILTDSGATSSAGTELEARSQSQLLQHQQQQQQQQQQQQQQQQESQQFRDIEQDIYDSYVPFSTDPQIGNGKTDVSKEHSKLNHEQKGHSSKPPKSGFEKHLTHGFASLGLKNKNGHGSSKSGNDLVGLDASSLNNQEKNTITTVRETLHKEPSDEQLLMRNLSTKNYGGSYPRMPGVAADEQTASAMANSNTYTGPSTNTNTNTNTNTNTNSPYRKKQKTPFITDDPNYVLEESQEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4L0</accession>
    <name>A0A1E5R4L0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Pre-mRNA-splicing factor PRP11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3804</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SF3A2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4L0">
        <property type="interactions" value="440"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4L0"/>
    <dbReference type="InParanoid" id="A0A1E5R4L0"/>
    <dbReference type="OrthoDB" id="10250970at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052092">
        <property type="entry name" value="SF3A2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031781">
        <property type="entry name" value="SF3A2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23205">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23205:SF0">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16835">
        <property type="entry name" value="SF3A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="U1-type" evidence="10">
        <location>
            <begin position="77"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="215"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="11">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ81849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="296" mass="33402" checksum="F0D6203C513016CE" modified="2017-01-18" version="1">MDYNDRVGSKKGGGGIASASIQNIHRRKQVEDVLIGERIPFTGEVAEADQAQTGKEDISKDEQELQRRNPYIYKNHSGKLVCKLCNTMHVSWTSVERHLKGKKHGVNLQKRNNILGSSSNXRIETPEEKLQREXQTEREAKIXEIQKAIXXAGKMAVEDFQVCNVENSLGEQGILLKIQYGDQSVWQDGTPPFVRVISGLEVASAXDEQEKTNDTKQQQQSGQKVNKDKSYLCXSFPPYENVAVEIPSGKQITFGGSADGVIEQHHNKSLTVDKLNKRACFWDKDNKQFFVQIFFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4L1</accession>
    <name>A0A1E5R4L1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">rRNA biogenesis protein RRP5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Ribosomal RNA-processing protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3631</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the biogenesis of rRNA. Required for the formation of 18S and 5.8S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4L1">
        <property type="interactions" value="1254"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4L1"/>
    <dbReference type="InParanoid" id="A0A1E5R4L1"/>
    <dbReference type="OrthoDB" id="412781at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00164">
        <property type="entry name" value="S1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05702">
        <property type="entry name" value="S1_Rrp5_repeat_hs11_sc8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05703">
        <property type="entry name" value="S1_Rrp5_repeat_hs12_sc9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05693">
        <property type="entry name" value="S1_Rrp5_repeat_hs1_sc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000103">
        <property type="entry name" value="protein RRP5 homolog"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000155">
        <property type="entry name" value="rRNA biogenesis protein RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000196">
        <property type="entry name" value="rRNA biogenesis protein RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045209">
        <property type="entry name" value="Rrp5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048058">
        <property type="entry name" value="Rrp5_S1_rpt_hs11_sc8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048059">
        <property type="entry name" value="Rrp5_S1_rpt_hs1_sc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 11 PRE-RRNA PROCESSING PROTEIN RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270:SF10">
        <property type="entry name" value="PROTEIN RRP5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23240">
        <property type="entry name" value="HAT_PRP39_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="122"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="339"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="512"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="602"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="696"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="796"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="901"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="1005"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="1090"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="1175"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="1263"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="63"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="167"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1415"/>
            <end position="1444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="174"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1420"/>
            <end position="1433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ81824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1729" mass="192823" checksum="B3FF890F984F7558" modified="2017-01-18" version="1">MAVKRKRDNTESPLTRSDETKAPAKSSLVQQDAATEMSFPRGGASVLTPLELKQVANEAASDVLFSKEAQTTSSKDGKPVSKKSKKNSKKSQDNXVENDEDIKTSSQIVQQQHISFRTLPVGSLLLGQVSRVHNHGLTISLPDNLTGYVPITNVSKQIDDILEKLNEDQMKDDEAQDSGYESDEEEESDIKKPAEQELPHLNDFFKVGQWLRCAVTKNTALENSKKVSKKIELTIEPSVVNKGLEKEDAQLKFSTIQCSVKSVEDHGAMLDLGLEDASDLTGFIAKKDLVDSSVKLVPGYVFLGTVSKYSGRTVSVTEKQLSSTKPVSTISSVDAIVPGQLVDFLVDSVKEDKGVFGKVFGLANAFLSISQMKTKNSKKFKDIFEIGSNIKTRVIANILYKGERVVLLSQLPATTEFDQTLKSLECLESFPIGYTFENKMAIIAKDSQYLYVQINDNLVGQIHFSKHSEESEEFXEKARVLGYNTVDNLFVLTNDKDQLAMEYLRSQDIPIGKILTGCEIVTVSAEKGIELKILNGHFKAMVPPKHISDIKLVYPERKFKIGSTVKGRVINVNPLNGKVFVSLKKTIVNADVEETPLVSTYDQLAPGLKTLCTVETFKDNGAVVTLLGNLRAFLPNKEISESFVKRPQDHLRLGQTVPVKIVNFDKENRKISVSCKIISQEKLEAQKHIIDSMQLGRSIVQCVVVEKTKDSAVVELKSDHTIRGVVYAGHLSDQRIEQNRSNLKKLKIGSELEGLVIDKDPKTKVFNITCKTSLIKDSQSGDLPLTFSDVTSKATNAELHGYIKTISSTGIFVAFNGKFVGLVLPSYSGEDRNTDLXKKFYINQSVTCYLLRTDEENERFLLSFKDPASKTDAKTPAGTSNTAIINPIDKSVKTMQDLTMGKIVTCKVKQVKRTQLNVILADSLQGRVDISQAFNSFSDIKNIKNPLAQFKKDDILKVKIIGTHNIKTHTFLPISHTSGKNAIFELSAKLDQEEIPKLSSFKVDDSELYGFVNNYSKDFLWLTINPQVKAKLNYIDLSDNTSDLNDIQSAFPIGSAISCKTVSVDNEHECLVTRSRFSSIITSYQDVKVGDKLPCRITKISDSYVLVNLGNGVKAISFITNALDDFKNDLKTTYHINQVVVGQVLAKDDEEQKINVSLIKNPDSIINSASDLQVGQVVDALVKNVTDKGLFVYLSSTINAFVPVSKLSDSYIKDWKKFYKPLQHVLGKVVTAESNKRISLTLRESEINGDIQILKTIKDIQKGDVFEGVVRNVTDFGVFVKLDKTVNVTGLAHVKEISDSKIXGDLSDLFGEGDKVKAYVLKTNPEKNQLSLSLKASRFKIQDAENDVDMDAEAENEDAEEVEEDEVMDIDYNDEASDDEDVKVNASNGLSGTQISLTNDGLSLSAGFDWTGSILNQANEDSDGEENDEDEDFTAERRKSKKNRASQIVEDKTIDIAARAPESVADFERMILGNPNSSVVWMNYMAFQLQLGEIDKAREIAERALKTINFREEMEKLNIWIAKLNLENTFGSPETLEEVFKQSCQYMDSYVMHMKMIGVYQMSAKVDEALSLFKAAAKKFGSEKVSIWVNWGEYLIDQQRADEAHAILASALQSLAKRDHIEVVKRFAQLEFKKGDPEQGRSLFEGLLADAPKKIDLWNVYVDQEIKHDDKTKVEDLFERIISNVKITKKQAKFFFKKWLDFEENKNDTKTAEYVKAKAAEYVQNAENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4Z6</accession>
    <name>A0A1E5R4Z6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein SST2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3528</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4Z6">
        <property type="interactions" value="155"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4Z6"/>
    <dbReference type="InParanoid" id="A0A1E5R4Z6"/>
    <dbReference type="OrthoDB" id="196547at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030695">
        <property type="term" value="F:GTPase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04450">
        <property type="entry name" value="DEP_RGS7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058855">
        <property type="entry name" value="RGS1/SST2-like_Fungal-DR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845:SF192">
        <property type="entry name" value="DOUBLE HIT, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845">
        <property type="entry name" value="REGULATOR OF G PROTEIN SIGNALING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25889">
        <property type="entry name" value="WHD_Fungal_DR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0734">Signal transduction inhibitor</keyword>
    <feature type="domain" description="DEP" evidence="4">
        <location>
            <begin position="327"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="3">
        <location>
            <begin position="715"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="5">
        <location>
            <position position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ81985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="741" mass="83509" checksum="EE21BD3103686EF8" modified="2017-01-18" version="1">MSSGSSCQIVSEKSSTLTKPRKKTLHETTSKCFSRTPKGLIFTKDIKDVYSLMVICMDLTKKPKRKLNNPFYTLTGSSSGSFTFCLQDAVDIMEKLRVDVEMKSTAVSISYSIKAEMSIQLIRIFMKAKLLHSPADRTRTEPKDKIFLQPTPKGVSILQKYVKDMGIKNVPAIIYSSLNSNQLFRFERSSVTDRIIYNEYFINLLFIRMMGTKPNVWAPTNENDPLPSLGQLLEYNDETFSFDCLDFSQGITNALMQQNQATQNNGNDKFLINNVSEETLRNEKRTSPLAHRFFTHPDSDSHVQYYTSNCGLRLLHNKIFGKGSPKTIPYSFTSKAIIQWIMDCTDVIYPKEAVTIAALMLKNGLIVPITGFPSTSVTSKFGTGKTQYYTMTTIGCDIISWNAELKKEVAFSMYGSRSSDXSSXDSAGGGADCEWTNDQNNAFKSISRXFSTNLKVATLAETLGDPGVRFLFRAHLEREMCVENLDAYVDFKKLLKKMALLQSLVESRMKAKLKNKRLQMKVAQKREKIKASAINVTTTINNALIKEVEDCLSLVYQLFSTYITTGAPRQLNLDYILREKISAVVLHPDSPITQSRSKDLVADSLARTTIKRPSSKMEIVLKAFTAPSKMEIVLKAFTDEELSKMNNDEFSIPHASALKRPEKPKSLQLTNSTTLLPDYDTSISTSVFFQDENPMTPTDEIIKHSVATLKLLFPLFEEVNEKLFKIMDSAAFPKFLESNSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R582</accession>
    <name>A0A1E5R582_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3677</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R582"/>
    <dbReference type="InParanoid" id="A0A1E5R582"/>
    <dbReference type="OrthoDB" id="5398685at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="57"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="82" mass="8897" checksum="AAD19C480040816A" modified="2017-01-18" version="1">MTSQNEGTDTPTEFTPGLLSDMLKKIQQGETTATQLENMLDKIEAQMASIIEEMGEPGATISGSSLDVETCEDELETEKTPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R584</accession>
    <name>A0A1E5R584_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Transcription elongation factor Spt6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g3657</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Plays a role in maintenance of chromatin structure during RNA polymerase II transcription elongation thereby repressing transcription initiation from cryptic promoters. Mediates the reassembly of nucleosomes onto the promoters of at least a selected set of genes during repression; the nucleosome reassembly is essential for transcriptional repression.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the SPT6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R584">
        <property type="interactions" value="1260"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R584"/>
    <dbReference type="InParanoid" id="A0A1E5R584"/>
    <dbReference type="OrthoDB" id="995477at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140673">
        <property type="term" value="P:transcription elongation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09928">
        <property type="entry name" value="SH2_Cterm_SPT6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09918">
        <property type="entry name" value="SH2_Nterm_SPT6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2740:FF:000002">
        <property type="entry name" value="Transcription elongation factor Spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000065">
        <property type="entry name" value="Transcription elongation factor SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.650">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2740">
        <property type="entry name" value="Spt6, Death-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.850">
        <property type="entry name" value="Spt6, helix-hairpin-helix domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3500.10">
        <property type="entry name" value="Tex N-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.140">
        <property type="entry name" value="YqgF/RNase H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049540">
        <property type="entry name" value="Spt6-like_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028083">
        <property type="entry name" value="Spt6_acidic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042066">
        <property type="entry name" value="Spt6_death-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032706">
        <property type="entry name" value="Spt6_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028088">
        <property type="entry name" value="Spt6_HTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035420">
        <property type="entry name" value="Spt6_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035018">
        <property type="entry name" value="Spt6_SH2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035019">
        <property type="entry name" value="Spt6_SH2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028231">
        <property type="entry name" value="Spt6_YqgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055179">
        <property type="entry name" value="Tex-like_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023323">
        <property type="entry name" value="Tex-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023319">
        <property type="entry name" value="Tex-like_HTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017072">
        <property type="entry name" value="TF_Spt6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037027">
        <property type="entry name" value="YqgF/RNaseH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10145">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10145:SF6">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14635">
        <property type="entry name" value="HHH_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14641">
        <property type="entry name" value="HTH_44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14633">
        <property type="entry name" value="SH2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14632">
        <property type="entry name" value="SPT6_acidic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21710">
        <property type="entry name" value="Spt6_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22706">
        <property type="entry name" value="Tex_central_region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14639">
        <property type="entry name" value="YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036947">
        <property type="entry name" value="Spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158832">
        <property type="entry name" value="Tex N-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="13" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="7 9" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="SH2" evidence="12">
        <location>
            <begin position="1259"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="95"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="145"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="165"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="205"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="221"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR036947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ82028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1454" mass="168057" checksum="95DA23F9CDC7D520" modified="2017-01-18" version="1">MSDTEEKNVANVDVNENEENDIIHGAEEQEGHSESEGEDVFDSSADDEEDEEDEEEAAKVKEGFIVDEDGSENEDEDEEQRKEERRRRKKRRTEEDVELDEDDLDLLMENAGYSNGPHKSEKGKLKRLKRAGRLDSDEEEENVEEGASGASASGSQRSTIINDFFSDDEEEEEEEELDHEEVDHYDHKRKGKPHQAPVAAKQAEFDDFIEDDEFSDEDEETRQQRLQERKKQREKNPLQVTGLSADKIDEMFEIFGDGHEYDWALDLEKEDEGEGIAEGSVQLEDIYDLQDLKQNLMTEEDMVIRRTDIPERFQELRKGLTDYEPLVNEEFDSEVMWISSKLASVKQVPEAYIQEFHEAIGNAINFISKENLEVPFVYSYRKNDISSKDTNGFILNETDLWEIVHFDVEFHSLLSKKKYIVKFYRDLDIHDSVVEEYLSSNDLNILQDIYNYLEFNYASEINATKNHTQNAAYHKKTHLKNSSYEKFKNSKLYQVVEKSGISTVQLGENINAGTQVYVVKDPEDDLSPEELVELTVADDVFFSSNLKLALETVEKYFALEMSQSPKIREAIRKNFYSYYVANVNLLPKGQKEIQLGSPYEDIKYAINRTPQSFLQQPETFLKMLEAEQQHYLTVEITMSSQEGFIDHVFDTCLESGGTNEVATKWNQFRKKVFVKAINKIFQDVSNEIKAQLQKVCENLVMKAIKREFLKKLDQAPFVNNHKKASRVLAVTCGNGKFGTDAIIGVLVNRKSLYEKDHKFVNNPFDRLNSKQFDDEFKNLCMDLQPDCIAINGPNVATQRFMKKLQDLIQYEKIENKHGNLLPVVFVDDQVATLYQHSKKSEIEYPNKNHLVKYCIALGRYVHSPLLEYANLSHEELCGLNIHPHQKLLSSELVNRSLETAFVDIVNLVGVDFSKISSDNPYYCSLLPYLSGFGKRKTIDFMENLQRLNEALVARQQLITKKILTKTIFINASGFIKMSWKSSSKMKNGRDRHSSTGGIDEDLLDGTRIHPEDYQLAAKVAADALEASPEEIQDHEETGEMGEFVEILSDDRDKQQKLESLDLNSFAKQYEEETGKRKFNVLRSIVYELIDGFEELRSDFHIMRPHEIFYNLTGETEESFHIGSLVPVKIDRYRNHNEIICSTNSNIECNISSTKHEGYYKRDVPVDELYELGKTYPSKVLSIDYANIKCEGSLLKHDTDLSSGGAGGVNGAIDGLVNTKINRDANTWDIAQEMADLAKEKELTMRERRAKRQHRVINHPYYFAMNGKQAEEYLKPKERGEFVIRQSSKGDDHLTITWKLDKDLFEHIDIKEEDKFNPLYLGNKLIVDGVEYNDLDHIIVEYLQNKIRLLNELTFSEKFKNGSKREVVKFIEDYSKVNPNRSVYYFSFNHEHPGWFYLMFKLNATSKLYIWNVKLTKNGYQLVNFNYPSVIQLCNGFKNLLKANSAKQRTQPPHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5C7</accession>
    <name>A0A1E5R5C7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein ECM27</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3544</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5C7">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5C7"/>
    <dbReference type="InParanoid" id="A0A1E5R5C7"/>
    <dbReference type="OrthoDB" id="407410at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051359">
        <property type="entry name" value="CaCA_antiporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12266:SF0">
        <property type="entry name" value="MITOCHONDRIAL SODIUM_CALCIUM EXCHANGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12266">
        <property type="entry name" value="NA+/CA2+ K+ INDEPENDENT EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="189"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="439"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="464"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="531"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="572"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="619"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="676"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="716"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="9">
        <location>
            <begin position="30"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="9">
        <location>
            <begin position="537"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="219"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="283"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="283"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="299"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ82106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="742" mass="83433" checksum="2780EEBD1A8AD54F" modified="2017-01-18" version="1">MNRFINHVYNGSLYTSSGVPLIILVPISIVHLIVCFALMGVLSSDFLASNINSLCDSSGILVTILLSFCNSSPDIISNFVAWTSSKTVQENTDFLAFSEVLGACGTIICIAQGLVLITIARIVRKKIFTIETVDDEDDNSDIPSNMDSHSEEELNRTVRHMLKKMLADLSFFAAAMCLMLICVFAGKISVFNCFAMLFLYGAYLFYNYKQHKLGQQVTKDSTTQPSASIQLEDYNTNDRGSLDDNTMEEEIVRYNYTHNPNYIQSIDYSVFLDLLEGSDSNXXESSNIEVSTNNNVGNKSKDHEPKDDKXSTRPFSAPEFTTGTNSERYTDGSRWPISEPGTLRPYSDFEQDNTREPLTSQEGIEQQEPIIFDPTVTSHKTXERSLYNQFHHRWTNFKRKKLYRQILIVSTTPMLLCIHASCPRLDKSVPATSKKFSQLICQSLLTPFFTVFSLAVFFGKFNWLVIGXLSAVLSIALIGLQLLMHNQLKEYTKVTLKPQVGKRRERLKDDTQDXLSESLQSIIDWEQEFRVFLKIFAIFGVVNSLLWIVILANNLIGIIQYYQEITXISESILGLTFFAWGNSIADILSNLAILKLYNTKMDNXNGDHKNKKLTSLLKFATISIGSCVGGCLINTMIGVGLNSLIAIILDKQXSLRVVYADPRFTWVFHSMTRKGFLHICVSSLAIVSTIFLLIGLLVVMLLGNRQCSAKTLCVSYLHKLGIVLVSFWMXTTLVNVLIEILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7G0</accession>
    <name>A0A1E5R7G0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Activator of C kinase protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3209</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7G0"/>
    <dbReference type="FunCoup" id="A0A1E5R7G0">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7G0"/>
    <dbReference type="InParanoid" id="A0A1E5R7G0"/>
    <dbReference type="OrthoDB" id="272077at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051726">
        <property type="entry name" value="Chitin_Synth_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430:SF3">
        <property type="entry name" value="ACTIVATOR OF C KINASE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430">
        <property type="entry name" value="PROTEIN SKT5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="501" mass="57144" checksum="30829DB1EF3B32BF" modified="2017-01-18" version="1">MVMNGNTSTSNAYPTHDLPYPVETLDYQSGGMANTTLGPPLSNTLSPIKQSGSPHPAIQQQNQPLTVPQGSSEPVQYPMEDFLLNYFQYRYEETVNDTKQNKKFTPYVQFQWCLALLQYSFQLGFVQRYNINCNVLPRILNDVEMKKNSTIILEHGIKVLSKLCDLKYGPAFYLMGCLYSHQLDLEFLSQFYENDPVARQIFPKKDGLTVYKKLCSGVQLKKILPLNDKKALEFYLESTHRTSDLLSITFGLYRSGVCLELGKGSDADLERAMELYELGWRDYKDQLCGYKFGLNVLKGKHRSSSHANDSIVVIKDAIKALHDVDTQQSLFELGKYYDNLGSQEVSKQQQQQQQQQSQDFLEKENKALMYYFRAAQKGYPLAIWKMGQIYEYGLLQCSVDADRSIAWYSKDMGFPLNLLALSGWLLTGGGSLEADEWQSFQMLERASTSGIGMNTSRIQYGMAIYYRYGIGCAVDKEKVRFYMTRAAKLGNSKAIEYLNTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7I8</accession>
    <name>A0A1E5R7I8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ABC1 family protein, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3345</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7I8">
        <property type="interactions" value="88"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7I8"/>
    <dbReference type="InParanoid" id="A0A1E5R7I8"/>
    <dbReference type="OrthoDB" id="1290869at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13971">
        <property type="entry name" value="ADCK2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044095">
        <property type="entry name" value="ADCK2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052402">
        <property type="entry name" value="ADCK_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45890:SF1">
        <property type="entry name" value="AARF DOMAIN CONTAINING KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45890">
        <property type="entry name" value="AARF DOMAIN CONTAINING KINASE 2 (PREDICTED)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="3">
        <location>
            <begin position="242"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="4">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ82880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="718" mass="83533" checksum="8F06D7D04C8EE4B9" modified="2017-01-18" version="1">MGFYSRAVGLTKQGVFPRLNHTLHGTRNYTXHFYTTNNHKEAXKNNNATPXTLVKSASRPSTLGSVYYLFGAFGVSCVGIFLYYRYERHQHILEHDLKINRTSPQGFLQPDLSHSDTYEMGLYVQSQKELEQSERAKRKRFEXSNFLIKYLYRFRYWTTDYVIEPCFIVGRFFRLLFIFTPVLVTFPLVSVLPSQLFSYDQFYSILLFCLQKAGPSFIKLGQWAASRTDIFDEKLCMTLGHLHSNVKPHSWKYTEKTLCENLQIDNLDEVFSEINKTPIGCGAIGQVYLGTLKSDLKNPIALKIVHPKVHKIISRDLKIIKFFANVIDHIPTMEWLSLPQEAEQFAFFMQLQLDFRIECCNLWKFNSQYKHSRQIKFPKYYPDYTTRDILFEEYIDNGVSMEKFLDMKKILANDDQSDIDHNMVPLFKKISDPFIDCFLDMLITYDFIHADLHPGNVILKFWDPQHKEYVSQEKINDLLEKYHACQETVEKTSHIESESSAEFSTKRELYSKKDQLKEQLIQILTEYEPKVCLIDVGLVTELNKRNRINFIALFNALAQFDGIKAGNLMIERSRTPETAINKDEFASKVNKLMQLVKKRTFTLGSISIGDLLEQMLSMVRSHHVRMEGDFVSVVVAILLLEGIGKQLDPDLDLFARSLPMLREFGMKQEGREALMLASDGHFLSMFKMWIGLELRNLLTTSVKELNELILTDQLSPFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7J0</accession>
    <name>A0A1E5R7J0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Sugar transporter STL1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3203</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7J0">
        <property type="interactions" value="88"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7J0"/>
    <dbReference type="InParanoid" id="A0A1E5R7J0"/>
    <dbReference type="OrthoDB" id="648285at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF73">
        <property type="entry name" value="METABOLITE TRANSPORT PROTEIN YDL199C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="276"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="403"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="427"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="461"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="493"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="522"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="110"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ82848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="683" mass="76386" checksum="75914C8361A269C4" modified="2017-01-18" version="1">MADHTSPNKHELQKTPVLTKPQPTASLFATGDKNTNTHGNPHTDQLLTDGPIYSPMPAHPPSYLTRDQESILSRTTPDGKRTPFSTDDIDDGSQLMLNSFLIGKPLLYFTTLFVSLGVFLFGYDQGVMSSIITTPEFKENFNYPSSAQISNIVAILEIGALLSSLSVGLKFGETYGRRRLIKFGALIFSVGGLIQTIGLNQVYLFVGRFISGIGVGVLSTCVPIYQSEISPPHNRGKLGCVQFTGNIIGYSSSVWIDYFCGSAFQNSKWSWKLPLLLQVFFGMLLFLGTFVIVETPRWLLNHDRDIEGYITLGKLHGEVDFMNSDTVKDEYMNIKETVLVSKINNEHHHSLWYYMKKYKQRFAIGMSSLMFAQFAGINCISYYAPLVFEQAGWVGRKAILMTGYNSIIYVLSTLPPWILIDYWGRKPLLMMGALTMGTSLIFISLSMLWNNXNWTPNLVVFGVIFFNWNFGMSFGPIAWLYSVEIAPITARSAMASASTAVNWFSNWVVGFLTPILQDAIGWKLYLLPATSCFISLYVVWKYYPETTGMKLEDMDSIFNDDRTSIVSLPKFTNENENTXALIAQPLTTTSTRNPIASETTSSXTPMMNILTANQLQTPTTNDILQSDSAKMVQQIGHPHFAHAENKMRKMLRRGSGTISLLYNKVFQNSSGQNIEYGTLNNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7J9</accession>
    <name>A0A1E5R7J9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ribonuclease T2-like</fullName>
            <ecNumber evidence="4">4.6.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AWRI3579_g3368</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Rnase which modulates cell survival under stress conditions. Released from the vacuole to the cytoplasm during stress to promote tRNA and rRNA cleavage and to activate separately a downstream pathway that promotes cell death. Involved in cell size, vacuolar morphology and growth at high temperatures and high salt concentration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vacuole lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the RNase T2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.19" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7J9">
        <property type="interactions" value="145"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7J9"/>
    <dbReference type="InParanoid" id="A0A1E5R7J9"/>
    <dbReference type="OrthoDB" id="435754at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005775">
        <property type="term" value="C:vacuolar lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033897">
        <property type="term" value="F:ribonuclease T2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01061">
        <property type="entry name" value="RNase_T2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.730.10">
        <property type="entry name" value="Ribonuclease T2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033697">
        <property type="entry name" value="Ribonuclease_T2_eukaryotic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001568">
        <property type="entry name" value="RNase_T2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036430">
        <property type="entry name" value="RNase_T2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018188">
        <property type="entry name" value="RNase_T2_His_AS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033130">
        <property type="entry name" value="RNase_T2_His_AS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057328">
        <property type="entry name" value="RNaseT2L_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240:SF22">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00445">
        <property type="entry name" value="Ribonuclease_T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25488">
        <property type="entry name" value="RNaseT2L_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55895">
        <property type="entry name" value="Ribonuclease Rh-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00530">
        <property type="entry name" value="RNASE_T2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00531">
        <property type="entry name" value="RNASE_T2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="RNase T2-like C-terminal" evidence="18">
        <location>
            <begin position="363"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00071169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR633697-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF25488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OEJ82858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="490" mass="55810" checksum="79CA8D0A01276DE1" modified="2017-01-18" version="1">MKDFWKILTGFGAASSILEDTPHKNPKDKIQNYQHNNXNKYSPSCPLNTPLSCSNSSAIADTCCFEYPGGIFLLTQFWDYMEPMNDEEKKVLEKKFTLHGLWPDNCDGSYEQFCDSGLNIVGYDIREMLADESAYSSPALPELEISGADLLADMXLFWKSNNNDDSSLWKHEYDKHGTCIKTMSPKCYSRWFDLDEDQEQNGQEVSWVQRXFKGNDADADDNAFKKEKDHENHELIKKRAVYDYFKTTMKLYKKMDTFEILKQSGIVPSEDKTYTRQEISDALKKGFDDKDVFFKCDRNNALNEIWYFHLVGQGSVLLNEAFVPIDSFRKYSNCPIDQIHYYPKGYKKKRPDHGGGGNDGKIGTGAIRISGGSKSSLGGFITRLGRWMSKGTEAKFDVFKSEFGNYLVRSSAGYCSVVGDSKELKCTGGRNNKNGATQFELNEKTGHLGYGGEYSWHSSXYPRGRQQSPVYHGASEDVEYTFELKFAKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7K8</accession>
    <name>A0A1E5R7K8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein IFH1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3190</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7K8">
        <property type="interactions" value="418"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7K8"/>
    <dbReference type="InParanoid" id="A0A1E5R7K8"/>
    <dbReference type="OrthoDB" id="3973039at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060962">
        <property type="term" value="P:regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018837">
        <property type="entry name" value="TF_CRF1/IFH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28057">
        <property type="entry name" value="PROTEIN IFH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28057:SF1">
        <property type="entry name" value="PROTEIN IFH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10380">
        <property type="entry name" value="CRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="243"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="761"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="975"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1208"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1382"/>
            <end position="1402"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1097"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="47"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="70"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="89"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="127"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="154"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="195"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="254"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="333"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="355"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="368"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="394"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="421"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="453"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="478"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="767"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="983"/>
            <end position="1000"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1402" mass="155601" checksum="B6290B58F0195782" modified="2017-01-18" version="1">MVPSISTTNKIFKSSTASTNGRPRRFSLLYSSSDEDDGESSNGNDDLSGKSKQTADRRNVSSDGFENIRKKLQSTLKRKLQNKSAFKDKSRRRSNSFRSKHTXRRSFSSKSVAHGLQQPQXERKSQQNESASSVFRSDSDLSALSEEEEKSSLNTGGRNSSMKNKQSVPGKEDGDDEDDDDDDDDDDDDDDDEYELQRQFEMQKKKNLKKKFMLLSANNQTYDRRGPNSDTSKLXHSTIEARSAMTGSKYXPMKTVPVSSSVSSKTVPLVNGVKNHGFIPSKRPMPLKTVSHGSPAGKKPPSFGRSKYSQNESDSELSEISSSDDSESESDEDTRIKDGLIREKYNKRARHSNSNDRSLLSGVSNGPSETQQNSSTXQHSSKDQHEIFNPNPLVATNSNGLKIAPTNNEMAIGDSSMSDISDVEIGESDGINDFDDLDENSLGIESDALQSSEEGDDEIEDDDDDNFDQEAYYKTIAEDDEEDLESSEDDTNAEDYDVFPILHEEEQNILEELEKDGLSFEGEEDILENQYIYDDSDGMSSENDDYMSEFNVPFYEEPNFTSLDYYKTKKNTEEDLDNDTLPLQLSTSSLLPLLHNYSNSTNFAATSKSGSVQTRKEKIMAKRKELMEKRKQRKLAKENNATFMKSPTPELLLLSNVKEDNSSTSAAPFVKNESDTEFYEKLFTNDGSAGLRPTKSPKKLGGNGYFTSDESSDEESVDFENFKYGKALQKKNDSFYSASEDDDDYDELLLEAALLDTENDDTDGIVTANNSEVGDNNSVKHFSTTDNTSLRSKASTTIHNSKSAASTPIKKLESSSESSSDFDEGDISMSHVFIDIDDLDPDAFYFQNTSEEDSEDGYSYKTQFHEGVKSDFARGMHSRAXVKTIDQTKGLLDQGANADLIAEDDDDVVYFIGGESTDEEENLPAASKTVRPVKPKGIVTLNTTGLNPPKLGMWKADDKKPFSIIDGLSTKSLHANQAKETKSSQQEENGSSSNNFSKSGTPGNVSGAAATKNDELDLNELLNMSSSSSENSDDDQNAHKMNYEQFFSNVNTSVPLSSFRNRGTIGDQMFSMSNDFPSIGRKVPIGYVGSMKTRKKLDKMKELQKKKQLRLKKLHKRKKLIKLEREKQRNAKEDALLGQLEENIXRQRESTQNQVLXKNTKXFEAQENXEXAALNAEPLVQSENFSAEKLQGSIKEEVLDQTDNSKNQEITQQDGFPSVHESPGNGAVANXYPDPKVTSDNVLPPANFAPANHISFVEDQQKNKNIGMNGLFNTAYQEMMIDGNEKQSVLMSNPGGIILDHSSGGFVNGVVIGNDDVDDVNLLASLTAPVSLDDSQNSQSMAALKRRRQSMAEANAERLRFTKAGLFSESALLDLEEIMQNTNSSSEQDFFSPESNNAEVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7V4</accession>
    <name>A0A1E5R7V4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3462</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7V4"/>
    <dbReference type="InParanoid" id="A0A1E5R7V4"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="86" mass="8941" checksum="6353EE994F61CF13" modified="2017-01-18" version="1">MIQGSSSSNYSIVDGLIVLGSIVDGLIVLGSIVDGSIVSDSNVENSIISDSNVEDSIVSDSIVENSIELNSLISFMSRVVAGYCIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R829</accession>
    <name>A0A1E5R829_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">DnaJ-related protein SCJ1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3475</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R829"/>
    <dbReference type="FunCoup" id="A0A1E5R829">
        <property type="interactions" value="506"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R829"/>
    <dbReference type="InParanoid" id="A0A1E5R829"/>
    <dbReference type="OrthoDB" id="550424at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072655">
        <property type="term" value="P:establishment of protein localization to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000001">
        <property type="entry name" value="DnaJ subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044713">
        <property type="entry name" value="DNJA1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43888">
        <property type="entry name" value="DNAJ-LIKE-2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184633" evidence="7">
        <location>
            <begin position="23"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="8">
        <location>
            <begin position="24"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="9">
        <location>
            <begin position="154"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="6">
        <location>
            <begin position="154"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ83044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="376" mass="42815" checksum="CCFDA8688E27D5A1" modified="2017-01-18" version="1">MKFSLYSLFVLNFFWLISSVFAQDYYKILGIERTASEKEIKSAYRQLSKKFHPDKNPDNEEAHHKFIEIGEAYEVLSDSEKKTIFDRHGAEAVKQAQQAGQQGGHQPFQDPMDMFHQFFGGGGGANGGGFQHRTPKTPTIQFQTPISLRQYYLGDTLTFELPLRTACRQCNGQGGKLETCGRCQGHGILIRQFRQGPMIQKIQQQCDLCNGQGKTLKEKCGKCHGQTVVQENKETTVDLPKGAIREYKAVLEGQGELYPGHEQGDIVVQFSESERDNMGYRRRGNNLYRTEYLSLKEALLGNWERELEFFDPTMNKIKLKRQSGERVIEGEIEVKSGFGMPFDDGNEEGDLFIDYHIIMPIGMDSAKASQKYFDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R8W6</accession>
    <name>A0A1E5R8W6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">L37</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">RP47</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">YL37</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3401</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R8W6"/>
    <dbReference type="FunCoup" id="A0A1E5R8W6">
        <property type="interactions" value="857"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R8W6"/>
    <dbReference type="InParanoid" id="A0A1E5R8W6"/>
    <dbReference type="OrthoDB" id="1166329at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.190:FF:000001">
        <property type="entry name" value="60S ribosomal protein L35a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.190">
        <property type="entry name" value="translation elongation factor selb, chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00573">
        <property type="entry name" value="Ribosomal_eL33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001780">
        <property type="entry name" value="Ribosomal_eL33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018266">
        <property type="entry name" value="Ribosomal_eL33_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038661">
        <property type="entry name" value="Ribosomal_eL33_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10902">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L35A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01247">
        <property type="entry name" value="Ribosomal_L35Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01105">
        <property type="entry name" value="RIBOSOMAL_L35AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 7" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00075866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00080139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00082606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ82973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="114" mass="12878" checksum="F6FF58CD49702F92" modified="2017-01-18" version="1">MTRLSNLEQQFQTVYVKGKHISYQRSKSVTNPNVSLIKIENVGTPADAKFYLGKRIAYVYRASKEVRGSKIRVIWGKVNRTHGNSGVVRATFRSNLPAKTFGASVRIFLYPSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA16</accession>
    <name>A0A1E5RA16_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3005</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RA16"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="216"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="15"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ83734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="267" mass="31048" checksum="A76CB44B93E01303" modified="2017-01-18" version="1">MFGVMNRDTSRTYPNLLNNNDSTSKSDSSGVIEDRKHKYSKYEKVHGTTATNAYASTIKKQTILREMRRQYKNMKTMEVREKKRRNNYNMAANGDNDGVQLTKTCXSPDETDNKALGSGDNNLGWYDAFTSDGDDVDETQQKAHFPTQKDNFLHASNSKTLDVDLTRNDSNGLDSYDSNDLNSYXSNDSNVGNYRYINKMSAKYQISVEEAELYYSQMLHEELQEEEHYLKEQENENKKKQLELQLQDLEEQEQMDIQYLLENFKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA33</accession>
    <name>A0A1E5RA33_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="2">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3124</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA33">
        <property type="interactions" value="255"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA33"/>
    <dbReference type="InParanoid" id="A0A1E5RA33"/>
    <dbReference type="OrthoDB" id="3349449at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039756">
        <property type="entry name" value="Lsb6/PI4K2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12865:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12865">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE TYPE-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="10">
        <location>
            <begin position="243"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="80"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="264"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="724"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="11">
        <location>
            <position position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ83769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="907" mass="104447" checksum="01FAFE66AF0C4F50" modified="2017-01-18" version="1">MSLEEQPLLRNQTWSPFEETDETNLLENGFSNINEKDGEESRYLRETGVMPVTSKKTRLNLFSSKNAQSSSSLVKTAIKNNKNNTNTTSKIRKGXQKHQRKQKLKKNKLKMSNQKIGRKNSLISMKSTMRWTKGLFQLNSTTLPEPGLYTRHFNDEIYSSSSSSSSSNNNAPNSIDDNIYFDEIPYEIQYSVFRATDKPLQSTGDNQSLVSTNSXTFTEDFELEKIEFLSLIQESIYTIQNYRIQPLRIQQGSSGSYFVYGRKPQFRDQPPENIDPNQXMSPSGDYDNRETQLSNEHIRSVSSLSAYPEFEILGVFKPKDEEPYGPLSPKWTKWLHRTFFPWFFGRTCLIPNLGYICESAASLLDKKLNIDMVPFTDTVVLDSSSFYPKKSNLIKNFFRKHFLHRSTVQLQRKVGSYQLFLQNYMNANDFFDIYPLPGMRGKYSTALHSDNXAFSHQSSFIWSANDGALLHRFRLQLEKLIILDYLMRNTDRGFDNWMIKMIRDPDSGNPIDIKIAAIDNALTFPWKHPDEWRSFPYGWLYLPINILNVPFHKKTREHFLQILTDVKWWEETFQDLTELFSRDSEFKMRLWEKQWSVLKGQAFNIVETLKSKDQGPLQLVRRTRCVVVDEHMKIEIVDPAFFKNLSSRIDIGGKXSENKSRTIFGIGTPELLNPPRNFTSPMKLSYESTKNVDESWRHLLNGEIDEQTWVNQATPVISQTXVGRENNGVQKSGKPFNETHVSDEAGGTSGSPRNTDFADANASFLNFPDEDVSAKXPPLRTVNKNRQFDNPFYHTEDDLGLNVSAVLPNSVNDVPSISKANSAGLKHILESDNGTPNTNEFKDTLQVSEDEDDEEDEDFSEYHDAVESSRDYQSISSIKPKTKNNKKTVIIERLKLVTSKPPVFTWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA90</accession>
    <name>A0A1E5RA90_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 18">3-isopropylmalate dehydratase</fullName>
            <ecNumber evidence="6 18">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16 18">Alpha-IPM isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17 18">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">AWRI3579_g2612</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 18">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 18">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="6 18"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RA90"/>
    <dbReference type="FunCoup" id="A0A1E5RA90">
        <property type="interactions" value="261"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA90"/>
    <dbReference type="InParanoid" id="A0A1E5RA90"/>
    <dbReference type="OrthoDB" id="2279155at2759"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009316">
        <property type="term" value="C:3-isopropylmalate dehydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01583">
        <property type="entry name" value="IPMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01577">
        <property type="entry name" value="IPMI_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000006">
        <property type="entry name" value="3-isopropylmalate dehydratase large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000007">
        <property type="entry name" value="3-isopropylmalate dehydratase large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000003">
        <property type="entry name" value="3-isopropylmalate dehydratase small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01026">
        <property type="entry name" value="LeuC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01031">
        <property type="entry name" value="LeuD_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004430">
        <property type="entry name" value="3-IsopropMal_deHydase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004431">
        <property type="entry name" value="3-IsopropMal_deHydase_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012235">
        <property type="entry name" value="3-IsopropMal_deHydtase_ssu/lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050067">
        <property type="entry name" value="IPM_dehydratase_rel_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033941">
        <property type="entry name" value="IPMI_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033940">
        <property type="entry name" value="IPMI_Swivel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00170">
        <property type="entry name" value="leuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00171">
        <property type="entry name" value="leuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002458">
        <property type="entry name" value="PRK01641.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004016">
        <property type="entry name" value="PRK05478.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009116">
        <property type="entry name" value="PRK12466.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822:SF9">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001418">
        <property type="entry name" value="ACN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10 18" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="15 18" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 18" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="14 18" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="19">
        <location>
            <begin position="9"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="20">
        <location>
            <begin position="540"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00014371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00033368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR001418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OEJ83830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="770" mass="84043" checksum="4D2D0F61D5F71116" modified="2017-01-18" version="1">MTAARTLYDKVFADHVVHKDESGSYLIYIDRHLVHEVTSPQAFEGLSNAGRKVRRTDCTLATVDHNIPTASRKNFKDLDSFIEQQDSKLQVKTLEQNVKDFGVTYFGMTDARQGIVHVIGPEQGFTLPGTTVVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLIQAKSKNMRIFVDGKLNPGITSKDLILHIIGVIGTAGGTGCVIEFAGQAIEDLTMEARMSMCNMAIEAGARAGMIKPDETTFEYLKGRPCCPSGSEWEKAVSYWKTLHSDEGAHFDFDVVIKGADIVPTITWGTSPQDALPITGCVPDPATVSDPIKKSGMERALSYMGLTPNTPFKEIPIDKVFIGSCTNSRLEDLRAAAKVVKGHKKADNVKLAMVVPGSGLIKKQAEKEGLDKVFEAAGFDWREAGCSMCLGMNPDILDPEERCASTSNRNFEGRQGARSRTHLMSPAMAAAAGIAGHFVDIREHQYKTDSAVEIAIESKEDAELQKAVYEHEKAPLPAGESSQEELADEIDDIPPSSQSATAAASGPTGMDPFVVLKGIAAPLDKANVDTDAIIPKQFLKTIKRTGLKSGLFYEWRFVKDANGKDKETDFVLNVEPYRNAQILVVTGDNFGCGSSREHAPWALKDFGIKSILAPSFGDIFYNNSFKNGLLPIRLPQEVILEKIYPLAQKGVEITIDLPNQQILDPEGKVLVSHFEVEPFRKHCLVNGLDDIGITLQKEEFITKYEANRRVQFSFLEGGSKLVKPIVGTKKSPYGNTAQEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAI6</accession>
    <name>A0A1E5RAI6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Putative ATP-dependent helicase IRC5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2772</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAI6">
        <property type="interactions" value="525"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAI6"/>
    <dbReference type="InParanoid" id="A0A1E5RAI6"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000015">
        <property type="entry name" value="lymphoid-specific helicase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="296"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="709"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="53"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="614"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="929"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="14"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="615"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="929"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="946"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="989"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="999"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="12">
        <location>
            <position position="622"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="12">
        <location>
            <position position="884"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ83907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1026" mass="117750" checksum="3EF26EA7464BBED8" modified="2017-01-18" version="1">MTTDHNQKGSLKPMQKRVSARLQKKQNNKGLGKNDAVGKIRYKEREEYELDQDVLLKDTVQKYGDSDDDRDEDNHNIDDLDNDLRVDEFLPSSFRLIHNKRNEKQFSNTEFMEEYQQLLSTADFEHLRLTDKKKPTNGGDDGIQDDVAEKNVQDHSETPEFDENEVDDAQIEQKVNELKEFVEQSKLYSQIIAEQLLQKEAKATEETPVREPLDHSSINFQVEEPPVRKRGRGKXLDDFFTRKSRKGTASDKIVLGGEAEQDSQNIEESWSIPIPKLLSPSITLKNYQREGFEWLVSLYSNGLNGILADEMGLGKTLQTLLLLSFIYENDTRGPFLIVTPLSTLDNWMNELAKFVPGMPRLKYYDREIRDHSDKYLRQGMVRSGGCIVTTYEMVIKDIDKFVGLDLKFLVVDEGHRLKNINCKLIQELKRLQSNGNRLLMTGTPLQNNMNELWTLLNFILPDIFTDFETFNKWFDYDQGVSNSVGASSASDSMMGKSTSIKLQKIINDELQKNLISNLHIILKPFLLRRLKRNVLSGVIPPKREYLVNCSLTPHQLDYYKYTLHSLLKEYLFVDGIKEFFICNRDKYGLNKVSNEVLLKYLQYRNATYMEKDFNDGDDDDDDDDDDNTHNTQNNNNNDELHEFFHKLDILLYNKYIVKQIKYKKYGNTIMQLRQIVDSSLLMFNPYHHYRTISGISLEKLLAESGKLKMLHQLLTQLLLPKGHKVLIFSQFYNMLDILEDYLNLLNLDFYRIDGGIKNEERKLQIEEFQLNASGNENTKKMPCSHKSSSQNQVNIFLLSTRAGGLGINLTNADTVILFDSDWNPQVDIQAIDRVHRIGQTKPVLVFRLMCDKTIENLQLLKIMDKRYLEKIIIEFGDFQIFKQDVVGEKGNNNNNNNSNNTGFSSSSSLLKTELNKLLMQRKSIMGFTSGNVASDTATTTTSSPXSPSPSSPSPSSPSPSSPSPSSPSSPSFPSSLDHGLLTQEELDILDDRSWKSYDTDSTMKNNHSQSLNSSNIRLFESSNSMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAN6</accession>
    <name>A0A1E5RAN6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">F-box protein MET30</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2748</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAN6">
        <property type="interactions" value="212"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAN6"/>
    <dbReference type="InParanoid" id="A0A1E5RAN6"/>
    <dbReference type="OrthoDB" id="5580488at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22147">
        <property type="entry name" value="F-box_SpPof1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000016">
        <property type="entry name" value="E3 ubiquitin ligase complex SCF subunit sconB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000715">
        <property type="entry name" value="F-box protein MET30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051075">
        <property type="entry name" value="SCF_subunit_WD-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19872">
        <property type="entry name" value="UBIQUITIN LIGASE SPECIFICITY FACTOR/HREP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19872:SF9">
        <property type="entry name" value="UBIQUITIN-BINDING SDF UBIQUITIN LIGASE COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="6">
        <location>
            <begin position="344"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="534"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="578"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="618"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="657"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="745"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="768"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="142"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="463"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="29"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="71"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="103"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="184"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="469"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="479"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ83957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="842" mass="94335" checksum="C3B375AE602D5DFD" modified="2017-01-18" version="1">MTLLRHSKRRSTTSINDTSDNTDLLDNTESQITNPQILNTTSGQAPTINPSQSIFPNSTGDNSSNNNNNNDIHTQPLRSDHNNNAISVSHREQSDLSLRSPPTFANKQQPPVTQKNKPLXSVPLIQNTESTQDIDMIHQSCSNTRISNTSPSNKRKLPHEEQXGCVDGDSASKVPKLSDPVPLSPNSSESADSRHNSFSNVHVSRNTGLSTNYTHNKPNINSISMTPPSLPQYSFTRYCYRHNPEYHIQQQDIIRQRRLSLASNIPQDSPKNVIRNLIKDDALFEKFQKEVGNDEENADILKFLNIFSGSIPQKRELLLKMILNLCCSPQLSFISSEVNSLIKIDFISTLPQEISMKILSYLDCSSLCNASIVSKTWKAMADDDRIWYHLCEQHIDRKCPQCGWGLPLLQMKRRRVISNNVCVNTSNEVSVCDTLNTAPINTPTAPHASLSMGQNQTALQGPVALNIGNDNNNNNNNNSTRSTALVSGPTSKTSGKPQGCVPGRTKTRPWKVVYKERFYVENNWRKGIYKKQEFVGHLDGVLDCKLSFNLNFNFLFTGSYDCTIGIWDINTGNLLRRLSGHSQGVKTLHFDGAKLITGSLDKTIRVWNYNTGQCVSTYRGHTDSVLSIDAYKKIIVSGSADNTIKVWHVESRTCFTLRGHTEWVSCVKLHPSSFTCFSCSDDMTIKMWDIRENKLLKTFQGHVGQVQKVIPLTIIDEENLISDIDYDRNYKDPELDAALKYPTHLLSCSLDNTIKLWHVQSGTCLRTHFGHVEGVWDIAADNFRIVSGSHDKTVKVWDLQSGKLIHSFGNMHDAPITCVDIGDSEFVSGDETGKVCMVHFDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAP4</accession>
    <name>A0A1E5RAP4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">V-type proton ATPase subunit H</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3062</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the V-ATPase H subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAP4"/>
    <dbReference type="FunCoup" id="A0A1E5RAP4">
        <property type="interactions" value="449"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAP4"/>
    <dbReference type="InParanoid" id="A0A1E5RAP4"/>
    <dbReference type="OrthoDB" id="10263554at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.150">
        <property type="entry name" value="V-type ATPase, subunit H, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004908">
        <property type="entry name" value="ATPase_V1-cplx_hsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011987">
        <property type="entry name" value="ATPase_V1-cplx_hsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038497">
        <property type="entry name" value="ATPase_V1-cplx_hsu_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10698">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10698:SF0">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11698">
        <property type="entry name" value="V-ATPase_H_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03224">
        <property type="entry name" value="V-ATPase_H_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032184">
        <property type="entry name" value="ATPase_V1_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase V1 complex subunit H C-terminal" evidence="6">
        <location>
            <begin position="348"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR032184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ83974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="475" mass="54692" checksum="25F15887B8666400" modified="2017-01-18" version="1">MFMLKDSVHFDTLKSQVKFQQTPWDSLVRSGDLNKTELDVLKNLFQNGVSDSSNKKDLDAVFKIIKTTDNIAILQNCYSLLVDLFISEKNKPIYEIVSAKNVCQEYLQTLLDSFKKIPKDASESNILVVETLGLYTAIVLLLQVQFDDAFFANFLSNVYFPLFKKTFGFVNELQYTLIRYLQELAAVSHYRSVIFSKDNDFVDHFFEIILSPISKNSNNSSSKALGTNQTVLPNNNLTIQIQYYSLLTIWLLTFENSIASKISTKYATSLLKLLRLVTVTIKEKITRLSISVLLNCCKNNKNFLKRMILLNNSKTLLANLSNRKFSDTELVQDLSTLNEMFDEITQELNSFDEYTIELDSKELCWSPPHVDTQFWYDNVDKFRENNWKLLKKLCDLITEFSEYSASSDSNAKASLILQVVLSDMTHIVEMLPDSVEFIEKYNAKIKIMELLNYPDSKVKYEALKTTQALVKNSFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAR2</accession>
    <name>A0A1E5RAR2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">V-type proton ATPase catalytic subunit A</fullName>
            <ecNumber evidence="2">7.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Vacuolar proton pump subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">AWRI3579_g2904</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20 26">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="19">Vacuole membrane</location>
            <topology evidence="19">Peripheral membrane protein</topology>
            <orientation evidence="19">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAR2">
        <property type="interactions" value="1119"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAR2"/>
    <dbReference type="InParanoid" id="A0A1E5RAR2"/>
    <dbReference type="OrthoDB" id="1676488at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006314">
        <property type="term" value="P:intron homing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18111">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18119">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00081">
        <property type="entry name" value="Hint"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000008">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1140.10:FF:000003">
        <property type="entry name" value="Vacuolar ATP synthase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001296">
        <property type="entry name" value="Vacuolar membrane ATPase subunit a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.16.10">
        <property type="entry name" value="Hedgehog/Intein (Hint) domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="entry name" value="Homing endonucleases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031686">
        <property type="entry name" value="ATP-synth_a_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007868">
        <property type="entry name" value="Hom_end_hint"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007869">
        <property type="entry name" value="Homing_endonuc_PI-Sce"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022878">
        <property type="entry name" value="V-ATPase_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607:SF1">
        <property type="entry name" value="H(+)-TRANSPORTING TWO-SECTOR ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607">
        <property type="entry name" value="V-TYPE PROTON ATPASE CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16886">
        <property type="entry name" value="ATP-synt_ab_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05204">
        <property type="entry name" value="Hom_end"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05203">
        <property type="entry name" value="Hom_end_hint"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="Hedgehog/intein (Hint) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="Homing endonucleases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="17" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0404">Intron homing</keyword>
    <keyword evidence="16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0651">Protein splicing</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-1278">Translocase</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="DOD-type homing endonuclease" evidence="23">
        <location>
            <begin position="487"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00029427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00058996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00082211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="OEJ83982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1120" mass="123206" checksum="0B69C9402E82C54C" modified="2017-01-18" version="1">MAGALENARKEIKRISLEDHSENEYGAIYSVSGPVVVAENMIGCAMYELVRVGHDNLVGEVIRIDADKATIQVYEETAGVTVGDPVLRTGKPLSVELGPGLMETIYDGIQRPLKTIKDMSQSIYIPRGVNAPALSREAKWHFKPSKTLQIGDHISGGDIYGSIFENSLLDDHKILLPPRARGTVKWIADEGEYHVDEIILEIEFQGEVSKYSMYHTWPVRVPRPVAEKLSADHPLLTGQRVLDSLFPCVQGGTTCIPGAFGCGKTVISQSLSKYSNSDAIIYVGCFTKGTQVMMANGEDEAIENIQVGDFVMGKDGLPRKVIGLPRGNDTMYEVSQLTGNRRDAEIHDLMDFVVSSDHKLVLKTKQNIKVATRSYNGNSFSSVSYFALEANKSGIDMVKNKTKVFGHHVHGGKSGADEKAAAFVAELDAQPFIEWVLEAKDYQQVSANVKANTTQLINPVFYESGKLGQLLESNGYSKNLAPEMAYLLGSWVGNGTMKSAQFSIDPKDTSFVKKINEYAQNFDLSSYSNDDHHCYSSNSNATGSPATNEYQFFVEKIGASQDENGDFTFDADFESGNELETSESSLVSWTEKGDLTVTFETAETHGKTVRKNNXGSNIFSRIAESFGVKTQTKCVPKHLAIDDIKVREFFLAGLIDADGCVRENQDGSKSASITTVHKSVSEGVVRVARSLGIEVSINTEDERIDSQGVRHQFSYCVSLNGAPLAGVLQNSALERNSTNSVTVXRKPVSFHFDLTEAAEKQDYYGITLDESSDHMYLLSNLALVHNCGERGNEMAEVLMEFPELFTEISGRKEPIMKRTTLVANTSNMPVAAREASIYTGITLAEYFRDQGKDVSMIADSSSRWAEALREISGRLGEMPADQGFPAYLGAKLASFYERAGKAIALGSPNRVGSVSIVAAVSPAGGDFSDPVTTATLGITQVFWGLDKKLAQRKHFPSINTSVSYSKYTNVLNKYYDSNYPEFPQLRDKIREILSNAEELEQVVQLVGKSALSDSDKITLDVATLIKEDFLQQNGYSSYDAFCPIWKTFDMMRAFVSYHDEAQKSVSNGANWSKLAEATSDVKHAVSSSKFFEPSKGYDEVHGEFEKLMSSIQDRFTESSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAZ5</accession>
    <name>A0A1E5RAZ5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2732</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RAZ5"/>
    <dbReference type="OrthoDB" id="128867at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="404"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="2">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="471" mass="55654" checksum="0813DAC1CF0B474B" modified="2017-01-18" version="1">MAGQPTLNLPGFYFDPEKKRYFKIALSSADAPPAQIFQREETEQEKYAKFQQLCQKNEYLCINVLQMTNNSTILEKFLRNRKLVMKAETFSKIHMVNRYNQKLSFDMIINSLHESEFFLFDLKEGSIWRLEENEHWGFPKWELTQVETYPYLVNCRQISVLGTETLFLTQKNDSFSVDENCGLFKHNDEFCRMISVGRFEAFYVRSKNHIGIRKFVDPKPHKDHQLIKLKNKSDIVSIGAAIKSMYVGLRDGTIMHYRFDQKRRCTSESLFSWNIHDHVEATNGLLYINKIDCAYLEAXYKVLVFTNRGILLYVQKETTHFLGFYNDLLSETLRLPSIIPQHQLLYMFCRAKTTKLMIYGRSNTPLKHFQVYMMIPEFMNDTPYESNLAFQNCFQQPFTDLWSLIKISNQRHQEEQELEEQQQENELEKDSIVLGMLESTMELALFLSHKGYVRCVHLHPEDFDTYIMSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB89</accession>
    <name>A0A1E5RB89_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mitochondrial acidic protein MAM33</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2869</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RB89"/>
    <dbReference type="FunCoup" id="A0A1E5RB89">
        <property type="interactions" value="563"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB89"/>
    <dbReference type="InParanoid" id="A0A1E5RB89"/>
    <dbReference type="OrthoDB" id="3972961at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.280.10">
        <property type="entry name" value="Mitochondrial glycoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003428">
        <property type="entry name" value="MAM33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036561">
        <property type="entry name" value="MAM33_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826">
        <property type="entry name" value="COMPLEMENT COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826:SF1">
        <property type="entry name" value="COMPLEMENT COMPONENT 1 Q SUBCOMPONENT-BINDING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02330">
        <property type="entry name" value="MAM33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54529">
        <property type="entry name" value="Mitochondrial glycoprotein MAM33-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="302" mass="33708" checksum="4CD5218166086017" modified="2017-01-18" version="1">MSKFLKSAKATSNLLRNSFISSVYTKPVVSTSSLMYLNGATGKTAKHNTNAKVWSRCFSSVNSVLFNENSKRVHDVLQSEIALEVEDPSVAELPEQLSQYMSNHKYEIVNRKPDASLVELLKSTDVENIHIFFDVSQIANLPSNFQNEAQSDLANGGSAASGSSDANAASGASEEMEFDEDELNEAFANVNVVVVKKSDNSAISMELLMNVMEGSFYIDSITPFKDSKIALDESADMEVKRDLLYHGPPFTNLDEELQDSLELYLQSRGIDSELANFVYEYADFQENNEYINWLKNMKSFFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBB8</accession>
    <name>A0A1E5RBB8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative elongation factor 1 gamma</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2498</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBB8"/>
    <dbReference type="STRING" id="56408.A0A1E5RBB8"/>
    <dbReference type="InParanoid" id="A0A1E5RBB8"/>
    <dbReference type="OrthoDB" id="249703at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050802">
        <property type="entry name" value="EF-GSTs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986:SF1">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="2">
        <location>
            <begin position="92"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ83863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="226" mass="26397" checksum="498C26D1ACB2784A" modified="2017-01-18" version="1">MFNSAVIYYFPGSPRYQLVNTLVEHCGLSDKITFKDINKCKPEFVKLFPEQKIPAFYEKEDDFKLTEVIAISYYLLSLGQDNQKENVLAGVKNKDKATILRWMSFFNQEFMKSATAIFYALQREDADEDETDVIAENYERLSEVLIPEVEEQLKKKNNFLSYLDHITLCDIFGALVFSLMFQAYLGKEWRSAHPAIMKWYNTVLNSEYLKGSYSPELLEKDAFFVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBE7</accession>
    <name>A0A1E5RBE7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="15">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g2785</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the peptidase C19 family. UBP8 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="15"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBE7">
        <property type="interactions" value="539"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBE7"/>
    <dbReference type="InParanoid" id="A0A1E5RBE7"/>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF33">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 15" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 15" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="17">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="16">
        <location>
            <begin position="141"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ83893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="487" mass="56045" checksum="C758C722D195DA64" modified="2017-01-18" version="1">MSCPHFDVIMKNNQIKSTFLNKYRMVKNLLQQSVDLNNNCDFMAHICNDCGEVHKGGNTFLCMHCTYIGCWGEKHFQQHLKQSKHNFGVNANTGELFCFRCENYLMQYDILSNIDQWSNWDLIEAASIALPNQFYTLNGLIGIHNMGSTCFMSSVIQCLIHNPYFAKWXFDQNHVLSCDLQNETACLSCAMQDIMNAAFSPNYDSDKPFVKLLQYCFKINTNFTGYTQQDAHEFWQFLLNQVHSDYKRCSKNVDLXSSDXSAQQQSSPLSGNSEDNEQCAHHLCNCISHTTFQGFLKSIITCPSCAKDSTTVDPFMDLSLEILNTNNKNEKQPLSTLYDCLDKFHKIEILQDYAFNCSHCSKKNIKSVSKKFVINKLPPVLTFQLKRFSHNLNGASEKLNHFVEFPRYLNMKNYLDFAKSSESFDLIYELFAVISHDGTVDSGHYVSFCKVTSDVWIKFNDADVQQVPEEDVLKEKAYLLFYIVRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBN5</accession>
    <name>A0A1E5RBN5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GPI-anchored wall transfer protein</fullName>
            <ecNumber evidence="8">2.3.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3116</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">A acetyltransferase, which acetylates the inositol ring of phosphatidylinositol during biosynthesis of GPI-anchor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Endoplasmic reticulum membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the PIGW family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.-.-" evidence="8"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBN5">
        <property type="interactions" value="548"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBN5"/>
    <dbReference type="InParanoid" id="A0A1E5RBN5"/>
    <dbReference type="OrthoDB" id="15270at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032216">
        <property type="term" value="F:glucosaminyl-phosphatidylinositol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009447">
        <property type="entry name" value="PIGW/GWT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20661">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS W PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20661:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS W PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06423">
        <property type="entry name" value="GWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017321">
        <property type="entry name" value="GWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 8" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="230"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="394"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="423"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="111"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU280819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ84307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="488" mass="54968" checksum="3DD6518EB17DE5AA" modified="2017-01-18" version="1">MADQADRLKDYMDSLKSRKEDFVSGLTGSTIPEINYISAVALTTYIAGKILNLASNKGNGQKFTLYMAIDYALNWLCLLLAVTLYANNVQLLHILILVPVALAAFAKSSNKSSKNTKKNAPRAKVDNKKTLKNKKNNTQTSSELKLVKQSFLTGYRSHMLIMTAIAILAVDFPLFPRKLAKVETWGTSLMDLGVGCFVFSNGVVSTRSVISEKLELKSKXNYLRKFVQSFKHGIILLALGLLRLYFTKNLEYQEHVTEYGVHWNFFMTLSLLPPFLVLVVEPIIMLTNVPRFIIALAIGLTTEYMLIHNEKMLPYLITSERDTLINANREGLWSFLGYCSIFLWGQTIGMFCLSSTPFKNNLYTVNPQSKGSKSVNKKLTKFDKLTTVSPLQGLFTWVCILGALSAIVFKYDPYTCSRRFANLPYVLWVALYNVCSIFFYALADYVCAYYKNSNFQNESVYEIPESLEAINSNGLLLFLLANVSTGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RCN3</accession>
    <name>A0A1E5RCN3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mitochondrial outer membrane protein IML2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3106</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IML2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RCN3">
        <property type="interactions" value="139"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RCN3"/>
    <dbReference type="OrthoDB" id="2154985at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019412">
        <property type="entry name" value="IML2/TPR_39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31859">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 39 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31859:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 39C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10300">
        <property type="entry name" value="Iml2-TPR_39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="236"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="45"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="236"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="265"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="856" mass="96024" checksum="DE18E98890C65E92" modified="2017-01-18" version="1">MFRLFRDKKAGSGSTSSLGSVANESNGSTANSSATSMHSVKSNRSSSSLATTGTSSTSKNNKAYTKQEQESELILNQAECFEIALQAMDYLLDDHAEKGYTILSEKAQEVALNQKHHPPGSEMILTLATGVIQFLEATLGFEANMINKARETMSKAEDQATKAQNYNVKKNLITSSYYPPGTEFKVTYTESCLLNALLMLFNESMMDSAKALYKLRKAYNNLQDLEKLITKAKMMQKKDNELKEKTGTEENGNDSEKTHNDDTKSMASSVASFSTANDENESEPSNTHEDKALGQKGNISQQSMSSLYSDVPFDMQKTDSSPNLIKLAEKVYDMRDQRLKGSHIGNTPASNRLRTQLGLQATMKNTVKDNKSNKFMYLNGDSSNSKPTVDEFIESGCDLCFGIIQVVLSLIPPAIGGVLSIIGFRGNREDGLRLIWRCVQERNIHGGLGLCGLLFYYNGPFQFVDDDFDIPATNSPASSLQQSMSSVSISSASSSSSSSLAATAGSNAREMTSHELLHPGKRLHDELLKARAVFPNSALWILQEGAVLAGNGKLDEALEVMDSIDLNLIEMKQVKSQLVFNRAIMLVFLQKYERAAEDFLYLLEINDWSHAFYTYFAGCCYLENYRMCQMGISTDDLDITKMDFYRERAASLIYKAPXYLNTKKFMAKNLPFDRFMSRKVXMFQNIAKKHNIEDPLDCIPISPVNELTYLYDGYTRMYEKNLLNSLKMVTEYKNPAIELQLEDQETIKEFLTAVIYRRIGKIAEGCEILDQKIIPKIIQSENNGKVKFXKLKDDPWLYPSALYERALFNWKLEDVKGLKDCKDWLQKSLDFAGDYELSSRIGMKTKAALSRVQDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDJ6</accession>
    <name>A0A1E5RDJ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GTP-binding nuclear protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1979</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the small GTPase superfamily. Ran family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDJ6"/>
    <dbReference type="FunCoup" id="A0A1E5RDJ6">
        <property type="interactions" value="1591"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDJ6"/>
    <dbReference type="InParanoid" id="A0A1E5RDJ6"/>
    <dbReference type="OrthoDB" id="48625at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00877">
        <property type="entry name" value="Ran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000131">
        <property type="entry name" value="GTP-binding nuclear protein Ran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002041">
        <property type="entry name" value="Ran_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24071:SF0">
        <property type="entry name" value="GTP-BINDING NUCLEAR PROTEIN RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24071">
        <property type="entry name" value="RAN GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00627">
        <property type="entry name" value="GTPRANTC4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51418">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ84956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="216" mass="24624" checksum="9B11C4EB8E317980" modified="2017-01-18" version="1">MAEQKETPTFKLVLVGDGGTGKTTFVKRHLTGEFEKKYIATIGVEVHPLSFHTNFGEIKFDCWDTAGQEKFGGLRDGYYINAQCAIIMFDVTSRITYKNVPNWHRDLVRVCENIPIVLCGNKVDVKERKVKAKTITFHRKKNLQYYDISAKSNYNFEKPFLWLARKLAGTPQLEFVSSPALAPPEVQVDEALMQQYQQEMEQATALPLPDEDDADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDQ2</accession>
    <name>A0A1E5RDQ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Serine hydroxymethyltransferase</fullName>
            <ecNumber evidence="10">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2347</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 10">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 10">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 10">Belongs to the SHMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="10"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDQ2"/>
    <dbReference type="FunCoup" id="A0A1E5RDQ2">
        <property type="interactions" value="870"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDQ2"/>
    <dbReference type="InParanoid" id="A0A1E5RDQ2"/>
    <dbReference type="OrthoDB" id="10265628at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000097">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF28">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6 10" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8 9 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="11">
        <location>
            <begin position="18"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ85030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="471" mass="52130" checksum="98D0DAF560F846A1" modified="2017-01-18" version="1">MSHYGLSESHLKMTTGSLHDTDPEVEAIIQGEVDRQKHSINLIASENATSSAVLDCLGTPMCNKYSEGYPGARYYGSCQWIDKMELLCQERALKAFNVTPENWGVNVQPLAGSPANLEVYQAIMKPHERLMGLYLPDGGHLSHGYQTENKKISAVSTYFESLPYRVDPETGLIDYDTLEANAILYRPKVLVAGTSAYCRLIDYKRMREIADKVGAYLMVDMAHISGLIAAGVIPSPFEYADIVTTTSHKSLRGPRGAMIFFRRGIRSHKKNGDPIYYDLEGPINFSVFPGHQGGPANHTISALATALKQAATPEFKEYQTQVLKNAKVLENEFKAKGYRLVSNGTDSHMVLVALREKGTDGARVEYVCEKANITLNKNSVAHDKNKSAIVPGGIRIGAPSMTTRGFSEEDFQKVAAYIDRAVNFAIELQKSLPQDANKLKDFKNKVDEGHEELETMKKEISEWAGSFPLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDU6</accession>
    <name>A0A1E5RDU6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SWR1-complex protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2033</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. Involved in chromosome stability.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SWC5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDU6">
        <property type="interactions" value="487"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDU6"/>
    <dbReference type="InParanoid" id="A0A1E5RDU6"/>
    <dbReference type="OrthoDB" id="445677at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011421">
        <property type="entry name" value="BCNT-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027124">
        <property type="entry name" value="Swc5/CFDP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48407">
        <property type="entry name" value="CRANIOFACIAL DEVELOPMENT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48407:SF1">
        <property type="entry name" value="CRANIOFACIAL DEVELOPMENT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07572">
        <property type="entry name" value="BCNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51279">
        <property type="entry name" value="BCNT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BCNT-C" evidence="5">
        <location>
            <begin position="259"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ85071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="340" mass="39175" checksum="A1F4C443BA1F840A" modified="2017-01-18" version="1">MSWSAKKETQESNSAQNKEHHLDSDAQEEVKQHQEGSQSDSEYDEEEDEDYNPEAQPDGKNGQFGNEGVDQEEDALNNDKEYQKLEQQYKSIESGGTVGGLVKTRRARLLEEEAERKQKYDNMNENLVTTSNVSSVWENLKKKANVRLQSNTTVSALDEAFKSDKLTDYPQXAFKNKDTSADMTNKIKIKRTYVFAGQQHTEEKYVLQNSAEAKEYIHSLKFKFSTDSSNASELKRDKTHXGETTENQNEMINGLRRPXKRPPILEKIIAGSLKPKLSTLEKSKIDWAGFVDNEGIYDELQQNTKAGYIQRQEFLHRVDKHKDVQYKKLRQQELAEKFKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDW8</accession>
    <name>A0A1E5RDW8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g2367</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDW8"/>
    <dbReference type="FunCoup" id="A0A1E5RDW8">
        <property type="interactions" value="1499"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDW8"/>
    <dbReference type="InParanoid" id="A0A1E5RDW8"/>
    <dbReference type="OrthoDB" id="1662883at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097435">
        <property type="term" value="P:supramolecular fiber organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000013">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000006">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013838">
        <property type="entry name" value="Beta-tubulin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002453">
        <property type="entry name" value="Beta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00228">
        <property type="entry name" value="TUBULIN_B_AUTOREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="14">
        <location>
            <begin position="47"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="15">
        <location>
            <begin position="246"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ85097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="451" mass="50144" checksum="298EFFEBB72E7A11" modified="2017-01-18" version="1">MREIIHISAGQCGNQIGAAFWETICGEHGLDYDGRYVGTNPLEKAGLPVYFNEASSGKYVPRSINVDLEPGTIDVVKNSKIGTLFRPDNFINGQSSAGNVWAKGHYTEGAELVDSVMDVIRREAEGADCIQGFQISHSLGGGTGSGMGTLLISKIKEEYPDRMMATFSVVPSPKTSDTVIEPYNATLSVHQLVEHASETFCIDNEALYQICQSTLKLEQPTYNELNHLVSSVMSGVTTSLRYPGQLNSDLRKLAVNLVPFPRLHFFMVGYAPLTSIGSRSFRSLSVPELTQQMFDSKNMMAAADPRNGRYLTAAAFFRGNVSVKEVEDEMLKVQNRNSSSFVEWIPNNVQTAVCSVAPKDMEMAATFIGNSTSIQELFKRIGKQFSAMFKKKAFLHWYISEGMEEQEFSEAESNMNDLVNEYQQYQEATVDEPVDEVYEFNDGTEPLRDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE98</accession>
    <name>A0A1E5RE98_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Imidazole glycerol phosphate synthase hisHF</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="12">Glutaminase</fullName>
                <ecNumber evidence="12">3.5.1.2</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="12">Cyclase</fullName>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g2056</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 12">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The glutaminase domain produces the ammonia necessary for the cyclase domain to produce IGP and AICAR from PRFAR. The ammonia is channeled to the active site of the cyclase domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 12">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 12">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the HisA/HisF family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">In the C-terminal section; belongs to the HisA/HisF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.2" evidence="12"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RE98"/>
    <dbReference type="FunCoup" id="A0A1E5RE98">
        <property type="interactions" value="289"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE98"/>
    <dbReference type="InParanoid" id="A0A1E5RE98"/>
    <dbReference type="OrthoDB" id="10254903at2759"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01748">
        <property type="entry name" value="GATase1_IGP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04731">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000094">
        <property type="entry name" value="Imidazole glycerol phosphate synthase hisHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004651">
        <property type="entry name" value="HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050064">
        <property type="entry name" value="IGPS_HisA/HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014640">
        <property type="entry name" value="IGPS_HisHF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010139">
        <property type="entry name" value="Imidazole-glycPsynth_HisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00735">
        <property type="entry name" value="hisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01855">
        <property type="entry name" value="IMP_synth_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235:SF2">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE HISHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE SUBUNIT HISF/H IGP SYNTHASE SUBUNIT HISF/H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036936">
        <property type="entry name" value="IGPS_HisHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12 15" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 12" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5 12 15" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="3 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="16">
        <location>
            <begin position="7"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="PRFAR binding" evidence="14">
        <location>
            <begin position="372"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="PRFAR binding" evidence="14">
        <location>
            <begin position="410"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="PRFAR binding" evidence="14">
        <location>
            <begin position="482"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="PRFAR binding" evidence="14">
        <location>
            <begin position="508"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="PRFAR binding" evidence="14">
        <location>
            <begin position="531"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="13">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="13">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="13">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="412"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent" evidence="14">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="477"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR036936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR036936-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR036936-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ85204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="558" mass="61769" checksum="E4FDDE2EA1213C9B" modified="2017-01-18" version="1">MSNKVYVIDVGSGNLRSLINAIKYLKYEVELVTKPEQIPSGADFDPETTKLIFPGVGNFGHFVDSIYAQKFDNAIKEYVANNGKIMGICVGLQAFFSGSEESPDSKGLNLFQDLALKKFDDSDKPVPEIGWNSVFGDELFYGLDPYKRYYFVHSYAAILPENLVNKNEINGWKIAKCRYGNQTFISAVQKGNIFATQFHPEKSGMAGVHVIDCFLKGDTKPFDTSIYTEEDKLKLKNDYSNYGLTRRIIACLDVRSNDNGDLVVTKGDQYDVREKSDNKDVRNLGKPVSLASKYYEQGADEVTFLNITSFRNCPVKDAPMLEVLEKAAETIFVPLTVGGGIKDVVDVDGTKYRADDVASLYFRSGADKVSIGSDAVYAAEKFIANGFKGDGTSPIETISKKFGAQAVVISVDPKRVYVNSPSNTKNKTFKPLKKGPKGEEYCWYQCTIMGGRESRDLGVYELITACEKLGAGEILLNCIDKDGSNSGYDLELIELAKKSVKIPVIASSGAGVPRHFKEAFLETNCDACLGAGMFHREEYTVNQVKEYLLEEGLKVRMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RED3</accession>
    <name>A0A1E5RED3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ankyrin repeat-containing protein YAR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1852</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RED3"/>
    <dbReference type="SMR" id="A0A1E5RED3"/>
    <dbReference type="FunCoup" id="A0A1E5RED3">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RED3"/>
    <dbReference type="InParanoid" id="A0A1E5RED3"/>
    <dbReference type="OrthoDB" id="10057496at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24188">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24188:SF29">
        <property type="entry name" value="GH09064P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="57"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="164"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="168"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="229" mass="25485" checksum="61A803E7DCE1A879" modified="2017-01-18" version="1">MSSTTETPATIFDAPLDQEDQDAIIFDARAGDLDSLKEIFESMVHPKLLLTCFQSYSKSTPLHMASANGHLEVVQYLYALAKDNLSSNDESGKAEELKKWVNLKNDSGNTALHWACLNGQLDVVKYLCDECDADPFVKNNADHDAIFEAENNGKEEIETYFLKKYDVEPEEKDTEEQEQVEGTDKDDDKTSGTSASDDMLHSVKVTEGTEIANVTIEANEAMAKMKLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REF2</accession>
    <name>A0A1E5REF2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA-directed RNA polymerase II subunit RPB11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1823</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal Rpo11/eukaryotic RPB11/RPC19 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REF2"/>
    <dbReference type="FunCoup" id="A0A1E5REF2">
        <property type="interactions" value="566"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REF2"/>
    <dbReference type="InParanoid" id="A0A1E5REF2"/>
    <dbReference type="OrthoDB" id="10248581at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06926">
        <property type="entry name" value="RNAP_II_RPB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.10:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00261">
        <property type="entry name" value="RNApol_arch_Rpo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037685">
        <property type="entry name" value="RBP11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009025">
        <property type="entry name" value="RBP11-like_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008193">
        <property type="entry name" value="RNA_pol_Rpb11_13-16kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022905">
        <property type="entry name" value="Rpo11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I,II,III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946:SF16">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13656">
        <property type="entry name" value="RNA_pol_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01154">
        <property type="entry name" value="RNA_POL_L_13KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RBP11-like dimerisation" evidence="6">
        <location>
            <begin position="31"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="121" mass="13912" checksum="65931A0D8E6BED06" modified="2017-01-18" version="1">MNAPDRFELFLLPEGASKLTIEPDTKAANAIIVDFEKEDHTLGNLIRAYLLKDPKVLFAAYKIEHPLFAKFKMRIQTIEGYDPKLALKSACNEIVKNLSKIHNNFETEWNLQTLAQEDVYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REK3</accession>
    <name>A0A1E5REK3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Thiamine biosynthesis regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2041</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REK3"/>
    <dbReference type="InParanoid" id="A0A1E5REK3"/>
    <dbReference type="OrthoDB" id="416217at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF32">
        <property type="entry name" value="ARGININE METABOLISM REGULATION PROTEIN II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="25"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="221"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="7">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="619" mass="71643" checksum="665BA9CB7E616F9B" modified="2017-01-18" version="1">MVNSNTDKKTKMVSNTSKKTRLFTGCWACRHKKRKCDQQKPRCSLCVKHNTVCSYDVKLTWNLNNIYLLEKSTNGFVSMDCVEKLKIKQNNKKNKKCCTSTREXEKANKKKKAFTISDRRFRVYKTLCPTIHTDDQYSQKXVDKRLSKLLNDLDILSEHVSFNDENSTTLGPYSVFKHIDDRENNKTVENNLPNASFASVSLSKNVKKIVQPCSSQFLAESMLHSQQQHQQHQQQQQQQQQQQQQQKNCPQQYIPQLSLFPNLLQPAPVMPSLLPGSDAFPLSRKDSNLSLSISPSVSSLESDDKSAANADDDLFNESHVFDDDEVNSEALQRHVRDFANSITTVSLGDVGKQHDLPPLEPQFENVDVNLDELSRLLTDDDHLVKTFSKNISNLTIELKYNISTTILNNLNTYLVQHNYENYNGLIDVMRWCLCNCEPNSKIPNTIIKSIYYHDMQVDMHYINLTFFILMWKDYNINNYTEWLYQWIQKCYTSLKSIAAANVSSTTAKSFNENTHSATLPKKLNFSLFLVLIEFYVCKDSMGYGIEIIPMDRLVTCFKLLNFLSAQQVSDMKFDIDLLKLKVQFDRELMRRSNSNKEMKKYFQIQLDIDSTLLHDVYEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REN3</accession>
    <name>A0A1E5REN3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Selenoprotein O</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1871</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SELO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REN3"/>
    <dbReference type="FunCoup" id="A0A1E5REN3">
        <property type="interactions" value="334"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REN3"/>
    <dbReference type="InParanoid" id="A0A1E5REN3"/>
    <dbReference type="OrthoDB" id="10254721at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070733">
        <property type="term" value="F:AMPylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003846">
        <property type="entry name" value="SelO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32057">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE SELO, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32057:SF14">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE SELO, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02696">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="311"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="57"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="739" mass="84390" checksum="65D0EAEDC97B83CD" modified="2017-01-18" version="1">MDPKMSKQFGVLRNINCFGKSNNFKVSSSFIENLTPDELLSSVSMASTLVRDEKFGSSSTSPSTSASAEEKHQMQIDEIMHTPRIISSGAHFAYCLPEIRRDYKTLFKSTKAYQDLQLPIENELAQSSNETSELNNKNDIEKIMNGQDLIITDKIYPYSMAYAGFQFGTFAGQLGDGRVVNLFELNGNAVQIKGSGLTPFSRFADGKAVLRSSIREFIISETLSAIGIPSTRALQLTLLPGTRAQRGEGFEPCAQVTRFAPSWVRIGNFDLQRWRQNAQGLIKLADYCIDYVMPKDMKTLPLSVFTPTAKGNANDSKNATQSPIPGMESSTEQQKKDDFVKTASFNPQDLTKYDEFFRKVIILNAKTVGYWQAYGFLNGVLNTDNTSILGLSLDFGPFSFMDRFDPRYTPNHDDFQLRYCLQEQPSVIWWNLQKLAQSLVVLLGSGPQYIDVFLQEPGKITEEMAQHMTERVQSMLEYNGNEYKFWVAFYTSKVMSERLGLKLFPEGDLFNESDYSKYQDIMNKVNTELLEPLWEFLYLTKIDYNNFFIDFQNYNDYTFFTETNGKKIFKKDFVTQILNTANNKELSPGLVEYLATGEFSQDNVLDIDTETDYREIEEKLRAWTGVYTEMYEQYNIDQTQGQVSLKLKRDIASKVNPVFTPRSFMFDEVIDDYVERQRDALHDPEAKIDNSLLEKLFLMSTNPYDPSKWKDSLHPATAKKWMAGVSEEDNVMQQCGCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REN7</accession>
    <name>A0A1E5REN7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Forkhead transcription factor HCM1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1706</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REN7"/>
    <dbReference type="InParanoid" id="A0A1E5REN7"/>
    <dbReference type="OrthoDB" id="5954824at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00059">
        <property type="entry name" value="FH_FOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000260">
        <property type="entry name" value="Forkhead transcription factor (Sep1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050211">
        <property type="entry name" value="FOX_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829:SF343">
        <property type="entry name" value="FORK-HEAD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829">
        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fork-head" evidence="6">
        <location>
            <begin position="225"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="4">
        <location>
            <begin position="225"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="111"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="312"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="111"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="124"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="319"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="789" mass="87988" checksum="D51272D6917F9ACE" modified="2017-01-18" version="1">MQNINQNTISLQETDKTLXNTKDITSTIHSDVVHTDNSYWNGRGKDXLGSSPLQMFESVSHITTPFEVTSSNQMDYISFENFKHISNAGSLESLESLKSLNGLEAQQSYLKPPLSPRLSSPVSHQNEINNTDTSPSRLTKKGTTEPNVNSLKSNKRSHSQLSSNSNSQTKSKKKIPISSSKTSVATPMVEIPLQKNYGSLEELLAEVHSTVSPEKXSGLYDASVKPPYSYASMIALSIMESGQGRLTLSQIYSWISTHFPFYKLGDSGWQNSIRHNLSLNESFIKGGKCADGKGHFWQIEPGQEYKVLKVQSKNGRNPSSTSTSNNSSSNKSTVDSGNKKKNIPSKNSLEKLKTNNAIXTNMQQKNTTVQLLTPLSSFSSSSLPLNVQPTHTAHLIPETPTSGDSWEWGAANNNTGNDHHHFDAFDTPMELLAKNDNDKFALRSEKKYDFAGNLATPKDTQHNDELFLMDTNTQLSKDSFLRNSTKQFNLNTTNTQIQSHFQEPNNATKNMHYWNNTFGTLSEPLITHQRGFSCSFNSSFELPNERSPPANSIDQQIQNKETLEEFKIELNDHEAESENHFDFTKTPKPAGRKLQETLEPERNFMLKNKHDDEFPKSIITETPYRFTNFLSSTPKTLDCVLHQEAFSKQFPENSNSGIPAGQTPRWKTPAVIDDFLTSPVTFKSPKNQNGLLGQVSSALLMHGSPDEHLHPTAGLLLSPTGRMSHSQKSTSSAKHTLLTSPRFDLINKRSRVLGHGGLFGVDVYSVLQRALNSQKQKKLLPHPELKDKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RET6</accession>
    <name>A0A1E5RET6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NADPH dehydrogenase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2456</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RET6"/>
    <dbReference type="FunCoup" id="A0A1E5RET6">
        <property type="interactions" value="869"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RET6"/>
    <dbReference type="InParanoid" id="A0A1E5RET6"/>
    <dbReference type="OrthoDB" id="3969599at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02933">
        <property type="entry name" value="OYE_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000138">
        <property type="entry name" value="NADPH dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045247">
        <property type="entry name" value="Oye-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893">
        <property type="entry name" value="NADH OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893:SF91">
        <property type="entry name" value="NADPH DEHYDROGENASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="4">
        <location>
            <begin position="18"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="396" mass="44766" checksum="BF2DBB5A117EA365" modified="2017-01-18" version="1">MTYSYVKDYKPVDLKNSNLFKPIKLNDKITLEHRITMAPLTRFRADDNGVPNKNDWAETYYSQRSVAPGTLIISEGAYVCAQAGGAPNAPGVYTQDQMKEWTKIFEKIHENKSYIYVQLWNLGRQASPAYLKSKNLKFVSATDNLYMDEQSKKEALDCGNELKGLTVEELETLKSDYVKAAKNCIEYGADGVEIHSANGYMLNQFLDPLSNQRTDKYGGSIENRSKYVLEVVDAIVDAIGADKVGIRLSPYGTYGTMSGGANPLIVAQYAHIIGELEKRGKEEPSKRLSYLHIVEPRVPNPNFSEGTHEYLDGTNEFVFSIWKGLVMRAGNYALDNKTALKDVNLNDRTLLAYGRYFISNPDLVERLEKGLPLTKYDRDTFYTKESKGYTDYPNYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REV6</accession>
    <name>A0A1E5REV6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Mitochondrial import inner membrane translocase subunit Tim21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2382</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the TIM23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion inner membrane</location>
            <topology evidence="1 11">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the TIM21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REV6">
        <property type="interactions" value="258"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REV6"/>
    <dbReference type="InParanoid" id="A0A1E5REV6"/>
    <dbReference type="OrthoDB" id="436405at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.320:FF:000002">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.320">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit Tim21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013261">
        <property type="entry name" value="Tim21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038552">
        <property type="entry name" value="Tim21_IMS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13032">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13032:SF6">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08294">
        <property type="entry name" value="TIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8 11" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="106"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ85439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="285" mass="32324" checksum="CCFE082B7A731429" modified="2017-01-18" version="1">MIKNIGHLQAAIRLDSSFAGPQAYKRLHTATQPFLQKAFLKNSYPKLNFHSAKXAPFSFQTHSLYAVQHHQQACRLSTIRKISQQAYSQANSKNPKQSAWQKVKNATTFTASGVLVLGAAGLAIVVVYLIFSELFLPSGDTHIFNRAVNLIEDDAISRNLLECNDTNVSKPDGTHHITKEKLKAYGETIKDDHWTRNRPIQSMRKVGVDGKERYFMRFHVESEKKLGTVYVEAKESETKFQPEFIVIYIDVPGKRRHYLVKPQISRTLNNTKGKGFLGLNWGPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REY9</accession>
    <name>A0A1E5REY9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein transport protein SEC22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g2004</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the synaptobrevin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REY9"/>
    <dbReference type="FunCoup" id="A0A1E5REY9">
        <property type="interactions" value="1056"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REY9"/>
    <dbReference type="InParanoid" id="A0A1E5REY9"/>
    <dbReference type="OrthoDB" id="1719357at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15866">
        <property type="entry name" value="R-SNARE_SEC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000065">
        <property type="entry name" value="SEC22 (YLR268W)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044565">
        <property type="entry name" value="Sec22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45837">
        <property type="entry name" value="VESICLE-TRAFFICKING PROTEIN SEC22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="190"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Longin" evidence="15">
        <location>
            <begin position="6"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="16">
        <location>
            <begin position="135"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ85455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="217" mass="25582" checksum="23C90DB0A7B27D58" modified="2017-01-18" version="1">MIKSTLIYREDGLPLCTSVDDDNTDVNLNELKKNIKKLLSKFTPQTENEATMEFKGELEAHYIRKNSIIYFVIVERNSPTPQISFSYLKDISVEFEHNYSNEYWKPTTRPYQFVSFDTFLQKTIKLYNDKRVFSNLDQLNNDLKDVKQIMTKNIEDILYRGDSLDKMSDMSYNLKNDARKYKKSAQKINLQLLISQYAPIAGVAFLVVLLIWWKFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REZ5</accession>
    <name>A0A1E5REZ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mediator of RNA polymerase II transcription subunit 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Mediator complex subunit 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MED9</name>
        <name evidence="9" type="ORF">AWRI3579_g1738</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the Mediator complex subunit 9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REZ5">
        <property type="interactions" value="137"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REZ5"/>
    <dbReference type="InParanoid" id="A0A1E5REZ5"/>
    <dbReference type="OrthoDB" id="4069563at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011425">
        <property type="entry name" value="Med9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07544">
        <property type="entry name" value="Med9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0010">Activator</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="197" mass="22206" checksum="4449BF6A8A9F33AD" modified="2017-01-18" version="1">MNTDIKVEDIDNGTVAXANTIENDALKKEAARAQEDAEMIDVASTEMKKEATNDSTRVLSKQGEPKEQEAKEATKHKGLEYIAAQLTTTEFIPQIFQSLNTLKMKDAINSSNNLEKQTANIKHKIKLCKNYILTEESTKAAGSXEAHTEDVLIRELLLKTPEQWAQYTEERHQLLATQQLAIDSLKEKIRDVLPEEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF36</accession>
    <name>A0A1E5RF36_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2404</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF36"/>
    <dbReference type="InParanoid" id="A0A1E5RF36"/>
    <dbReference type="OrthoDB" id="2444812at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="2">
        <location>
            <begin position="16"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="79"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="205" mass="22448" checksum="F658F2E63A0014A3" modified="2017-01-18" version="1">MNQPHSENNXPILLDPKKWKAVWDKEYKTWFYVDLSTGQSQWDAPKGTTFRPAAPPSGGAKDAGAPPAYSAAVNNNASPQNQQQQQQRFQQQPYPQQQGYPQQQMYPQQQMYPQQQMYPQQPMYAQQPMYAQQAQRTSNGSRFGGMGMGAGLLGGALGGFLLADALDGHDSYEQGYDQGYDNGVDNGNDFGGGDFGGGDFGGGDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF49</accession>
    <name>A0A1E5RF49_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">rRNA-processing protein FCF2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2387</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RF49">
        <property type="interactions" value="383"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF49"/>
    <dbReference type="InParanoid" id="A0A1E5RF49"/>
    <dbReference type="OrthoDB" id="3972693at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039883">
        <property type="entry name" value="Fcf2/DNTTIP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014810">
        <property type="entry name" value="Fcf2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21686">
        <property type="entry name" value="DEOXYNUCLEOTIDYLTRANSFERASE TERMINAL-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21686:SF12">
        <property type="entry name" value="DEOXYNUCLEOTIDYLTRANSFERASE TERMINAL-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08698">
        <property type="entry name" value="Fcf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fcf2 pre-rRNA processing C-terminal" evidence="4">
        <location>
            <begin position="129"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="74"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="245" mass="28854" checksum="0DFF8E2CC88771F2" modified="2017-01-18" version="1">MSNIDEKKLDQLFELLKNTDNMKSESNNQLSVDVSFADELDDNKIHFDDDLEDNKREQKFLEIEKNLKKLPKLENNFDKLSNPKSLNNDERANSKSSGQFSKISDPIEILNQRKGTKSKKXKGEEALPQTDTEKWFHIGKTEMTPQVKRDLLVIKHRQALDPKRHYKKDKWVLPKNFQVGTLVEDKSEFYSSRINKKQRSSTILGSLVNETDTNKYFKRKYAEVQDIKTRYGRSRHNYKSKSNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFJ7</accession>
    <name>A0A1E5RFJ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RFJ7"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="459"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="460"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="505" mass="56991" checksum="8C38E01AF5E4440E" modified="2017-01-18" version="1">MIMNDSIIQAIERFGVDCNSLKKPILTTTTFELYAVPEIHAGQQVYVKVETIPSILLNGNAAVSSTSSVNKDCEQAKCTSLMKKMTNYLNYGICKRENIVRKETLDNLEITLYCLDLQPANQQQSCSSXDLEHLLDQSRTNSNLQECPDYFYTSTDSASILSKEGHSSSNLKNTNSVDQTNNEVHVQWVERKTGTYLEDPDALRKKKGHNCKKTMKIRKSIGHIVCKNSRLPKSPMPFLIKEHTQNTASHFNSHKESSLKVAFKNTHYDELLKATAYNKSSNSSLNSLLYDTFEDVDEYDSEYLDSNADSEDLEEENSDSDLDTETECDDLCCDDDDDDDDDDELSCDEDDDLDEDNENLRSVHDAEHRGFASTAKVCLNASFENAPEMPKRVPKTTVFQLGSGPLNSNITNTEMEMSVKSPIHHTFPRTPVPPSCEDSSRTSINNVLFLADNSTFMPENKFEDNKHEQEQEQEQGNRKGSWASVSLNLDQSSVLNRRKHKVIPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFL5</accession>
    <name>A0A1E5RFL5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Structure-specific endonuclease subunit SLX1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1765</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalytic subunit of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Forms a heterodimer with SLX4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the SLX1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFL5">
        <property type="interactions" value="412"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFL5"/>
    <dbReference type="InParanoid" id="A0A1E5RFL5"/>
    <dbReference type="OrthoDB" id="24645at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033557">
        <property type="term" value="C:Slx1-Slx4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10455">
        <property type="entry name" value="GIY-YIG_SLX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1440.10:FF:000006">
        <property type="entry name" value="Structure-specific endonuclease subunit SLX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.10">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03100">
        <property type="entry name" value="Endonuc_su_Slx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027520">
        <property type="entry name" value="Slx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048749">
        <property type="entry name" value="SLX1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050381">
        <property type="entry name" value="SLX1_endonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20208">
        <property type="entry name" value="STRUCTURE-SPECIFIC ENDONUCLEASE SUBUNIT SLX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20208:SF10">
        <property type="entry name" value="STRUCTURE-SPECIFIC ENDONUCLEASE SUBUNIT SLX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01541">
        <property type="entry name" value="GIY-YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21202">
        <property type="entry name" value="SLX1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00465">
        <property type="entry name" value="GIYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50164">
        <property type="entry name" value="GIY_YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 10" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2 10 13" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 10" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GIY-YIG" evidence="12">
        <location>
            <begin position="17"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="109"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ85699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="345" mass="39809" checksum="8B44ABAF60320ABE" modified="2017-01-18" version="1">MAXQEGINNNXNNVGVDFYCCYLLRSLPKQQSFYIGSSPNPVRRLRQHNGDLSRGGAYRTKKLGTRPWEMLMAVYGFPSKSAALQFEHAWQHVYQSRFVKEWSNDCLEKTPSPTKSQTKNGXQKQTQSQSQSQFTKNSRGIHYKVAGARILLHTEHFKPMNLXVRMFNKDALKIWDTNKYKLPNXVDEKNVFLEQPEKCALTLPGKDLELIDIEQADESNREVLERANTNMRLLSNFYSHFIKGVNDYYEKSKDKLSYGDLACDYCCGNFNYIEDDANLKPRVVFCYKPGCNFKAHLKCTYETFPKPCGKLIPIKGECPICHETLDWVFLIKNSHKIKVKLGISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RG24</accession>
    <name>A0A1E5RG24_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Outer spore wall protein RRT8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2243</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RG24">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RG24"/>
    <dbReference type="InParanoid" id="A0A1E5RG24"/>
    <dbReference type="OrthoDB" id="10012223at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005619">
        <property type="term" value="C:ascospore wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059112">
        <property type="entry name" value="CysZ/EI24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052786">
        <property type="entry name" value="Spore_wall_assembly"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34292">
        <property type="entry name" value="OUTER SPORE WALL PROTEIN LDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34292:SF2">
        <property type="entry name" value="OUTER SPORE WALL PROTEIN LDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07264">
        <property type="entry name" value="EI24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="162"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ85523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="357" mass="41036" checksum="2401A199AECF72AB" modified="2017-01-18" version="1">MSSQLIITNHKSYSNELKPHNINENVTNDQNNVNSEKLKSHKNKAVHENNAAGKVSMLKKIFENLSSYVMKEISLVTSNLPKDFFVKMTYLYPLQGFIEFLTTPLYWEFLLPMLYPYFLLTTVIGILYYFLIFPIILVNLTMTMGVIGFVIAHIHFFLEANAISLAIAKIILFPSTNAIFFSLVLENHNQQDYLVEAKKQLTSRGAFQNPEKHGSFXKIWKQILEDDXILDLSKDEHDHRYWVHVAPLQILSLFHRMLRFCFLFGLSFIPIVGPILVLCLTSPARAQNCLHEYFVLENYSKDTINNVRRANYGQFLAFGLVAGLLETIPYLSLVFEYSNLNGAALWANDLIEKKKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGI6</accession>
    <name>A0A1E5RGI6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MICOS complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1083</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGI6">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGI6"/>
    <dbReference type="InParanoid" id="A0A1E5RGI6"/>
    <dbReference type="OrthoDB" id="2399148at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044284">
        <property type="term" value="C:mitochondrial crista junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019166">
        <property type="entry name" value="MIC26/MIC27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033181">
        <property type="entry name" value="Mic26_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28268">
        <property type="entry name" value="MICOS SUBUNIT MIC26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28268:SF1">
        <property type="entry name" value="MICOS SUBUNIT MIC26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09769">
        <property type="entry name" value="ApoO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="244" mass="27785" checksum="BA0952FFD7E09AC3" modified="2017-01-18" version="1">MARNFYTEIDEKTIFPPSQXNVEHKTYIPASFPSENKSXQSXQLESNNGNGFLSKIFGTNQLVDGISIRNFTPITDFFTAVRSSLNKTYNEKIEPSLEQKANTYYKYESHVMQNYVDPLYTDKREILLPGLAYTAIAYMTGAIMSRNLKPGILRLPLPLLLSALTFKYTLPSTFENTKNVLYKIESNVTSDKFLSKQHELVQSAETLEKDTVASYHKTVTKIEDINTSVSTNWEKLKKFTGLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGR0</accession>
    <name>A0A1E5RGR0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LicD/FKTN/FKRP nucleotidyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1098</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGR0"/>
    <dbReference type="InParanoid" id="A0A1E5RGR0"/>
    <dbReference type="OrthoDB" id="444255at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009644">
        <property type="entry name" value="FKTN/MNN4/W02B3.4-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007074">
        <property type="entry name" value="LicD/FKTN/FKRP_NTP_transf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407">
        <property type="entry name" value="FUKUTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407:SF28">
        <property type="entry name" value="RIBITOL-5-PHOSPHATE TRANSFERASE FKTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04991">
        <property type="entry name" value="LicD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="6">
        <location>
            <begin position="467"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="886"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="7">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="905" mass="106343" checksum="D216C8977E61C0C7" modified="2017-01-18" version="1">MRYFASMFFRRHKLLKHICLSLFILVTTIAVLHKLEAHKNEQLQSYXKNLPSLVDNVLGTSGSSSLDSSSLTPTYQSKNINDIAPSKINSAKTGDIITHLKNLYRNTKFNKHNVESNIQSTAFEYPDIIQRDLYYQQQQXTEESDSTKSLLPSKNXKTYXFDIKRTLASYYELIDADPSVEELPFNWYDWRKNVGDNLNKYIETKDYLEKLSCTKLYNDGKDEKKQMDHDQIRKYCADVRSKEEMIQNVHLDLESDGINVEYLYDHVSEKAMHILTSSYLYNGKMQPLSLTFLDSRNGDIKQYKTKLSTPFFQTSESDVIRNKLEKSDKSDKSDKSGEIPSSSITNILDLAWNKVKYHDNEIKVGSTFDRFDTKFDPNRKESTENNKNVLQVHQEHFNKAKSFVDHPSTNKNXSEFRYFLEARDFDAHIYPQFFNYPLDHNVLTKGQSLHDLARVWFKFIEGTKQASSWLAHGSLLAYVYNSESFPWDDDHDVQMPINDLILLAEKFNQTLIVSENGSPMFFDVSPYILKGRHYSDEWYNHIDARLVDITSGYYIDITGVGYSGENNNINKNDHLRFVDERKALDDKSITDVMQFHKDHGIVNCKNKHFSLIDELVPTKKILFGGVPAQVPHQSLPSLKSEYTLSKLLYAKTHDFRYHNYVPELRAWIPTSWTKQKFSXSYFERSQLGQTDVETLHENLLKTIKQNQEDKDYIEFYQDETLIYVLKLQHTTLNRLQTMNIEMDYTKNLLQKEQLIYDINHWPLVERSFRSRLIQQFHDNLFXKTLDHLSIGNFDEYNKLLLNISSETDQQKGFQSKEQMVNFLKNSVIKDEIDHYIEIEKKYHITENYDFIKSLETKNYYKDETVPSDLNTILNKEVPKIYKLRKDKDDEEKSEKPKPFVKDYNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGT8</accession>
    <name>A0A1E5RGT8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Zinc-regulated transporter 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1497</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGT8"/>
    <dbReference type="FunCoup" id="A0A1E5RGT8">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGT8"/>
    <dbReference type="InParanoid" id="A0A1E5RGT8"/>
    <dbReference type="OrthoDB" id="262547at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF210">
        <property type="entry name" value="ZINC-REGULATED TRANSPORTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="432"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="463"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="507"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="129"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="547"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="547"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="562"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="590"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="628" mass="68767" checksum="A6F6D969A15A38D1" modified="2017-01-18" version="1">MTLIIPKWLMYTLISSFLCILGVLTVPLISVGVGNWSRGRKSAVQLQTHRDSINARLLNYGLSMSSGSMLLTSLYKMLPKIKSGSGSNAAGNKYVVTFGCLFGVVLSLFLNFVVHAYTSESLIHCAHSHNDDENEHGSETDSNHRDHAHVNRDVHTNKPTKREILGPDLENQVDQHHHLQNTPVLQKKKSSIRDVLSKKKSGVIGGCEPKSCDLNNELTTKLSPQEGQVSSLATTGGAVANTTSTSKDSFTSAPMCLENSIGYDLQNLDQYRNNFFASANQNVAKNTTESAPLLLHHVHHQDSHDEDSHIHTNESVNHAQHSYGSTHSHNTHNELPRTSSFNVLEPDLLVNGSTANIHEKHNDDHHHHMETPFSKLMSIGLQTCVALTLHKLPEGLILYFTNNGAENNPNHGKHFGNSAPSDKDDFDSSSKLGFSIFIALAIHNFIEGFAMCLPLYTALSSKIMAIGLISILAGGSQPLGAGLGYMIHKYYSHSEYFQENGEMLQELLLSITSGFLLVISLQMFQTAIGFSDAHSHAMSSAQAIDTDDMHEHSHGHESRLDSSFQNTNSPSSGSSFGSYESHNLSHEEPTFQNKNPSSANNDMHTSGTTCVKWCCFGAILILSSGLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGT9</accession>
    <name>A0A1E5RGT9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ribosomal RNA small subunit methyltransferase NEP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1599</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase NEP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGT9"/>
    <dbReference type="FunCoup" id="A0A1E5RGT9">
        <property type="interactions" value="990"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGT9"/>
    <dbReference type="InParanoid" id="A0A1E5RGT9"/>
    <dbReference type="OrthoDB" id="269804at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070037">
        <property type="term" value="F:rRNA (pseudouridine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18088">
        <property type="entry name" value="Nep1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.10:FF:000003">
        <property type="entry name" value="Ribosomal RNA small subunit methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005304">
        <property type="entry name" value="Rbsml_bgen_MeTrfase_EMG1/NEP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636">
        <property type="entry name" value="NEP1/MRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636:SF5">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE NEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03587">
        <property type="entry name" value="EMG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ86094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="259" mass="28704" checksum="ADFC66F70418F4E3" modified="2017-01-18" version="1">MVEDSKVRDANNKEKIEHGYSRATLPASLIPQAPVVLTSKDKDAQRMIVVLSQASLDSHKVSTSSVSGQDKYALLNCDDHQGFLKKTGRDISEARPDITHQCLLTLLDSPINKAGKLQVYIQTSKGVLIEVNPSVRIPRTFKRFSGLMVQLLHKLSIRSINSQDKLLRVIKNPITDHLPTKCRKVTLSFDSKVVRVQDYIQTLDDDESICVFVGAMARGSDNFADEFVDEKIGVSQYPLSASVACSKFCHGAEDAWGIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGV0</accession>
    <name>A0A1E5RGV0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">26S proteasome regulatory subunit RPN13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1223</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGV0">
        <property type="interactions" value="204"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGV0"/>
    <dbReference type="InParanoid" id="A0A1E5RGV0"/>
    <dbReference type="OrthoDB" id="340431at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061133">
        <property type="term" value="F:endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.70">
        <property type="entry name" value="Proteasomal ubiquitin receptor Rpn13/ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006773">
        <property type="entry name" value="Rpn13/ADRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044868">
        <property type="entry name" value="Rpn13/ADRM1_Pru"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038633">
        <property type="entry name" value="Rpn13/ADRM1_Pru_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12225">
        <property type="entry name" value="ADHESION REGULATING MOLECULE 1 110 KDA CELL MEMBRANE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12225:SF0">
        <property type="entry name" value="PROTEASOMAL UBIQUITIN RECEPTOR ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04683">
        <property type="entry name" value="Rpn13_ADRM1_Pru"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51917">
        <property type="entry name" value="PRU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4 7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pru" evidence="6">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="151" mass="17144" checksum="4733D9CD9235A144" modified="2017-01-18" version="1">MSFPIITFKAGVCDFDEETKTITPVAMGGSVTIYQIKEDTEDXDNAEEDVAMNDAAEEDEDDMLIYEFVWKPTEASAKGRETDSYTIIPGENVWKQIESCKDGRVFALVFSSGEKKFFWLQDKVEDGYPKNHLSKQDLKNVEIVQKIMKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH83</accession>
    <name>A0A1E5RH83_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Actin-related protein 2/3 complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1322</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Arp2/3 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
            <location evidence="1 6">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ARPC3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH83">
        <property type="interactions" value="960"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH83"/>
    <dbReference type="InParanoid" id="A0A1E5RH83"/>
    <dbReference type="OrthoDB" id="200404at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1760.10">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007204">
        <property type="entry name" value="ARPC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036753">
        <property type="entry name" value="ARPC3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12391">
        <property type="entry name" value="ARP2/3 COMPLEX 21 KD SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04062">
        <property type="entry name" value="P21-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016315">
        <property type="entry name" value="ARP2/3_P21-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69060">
        <property type="entry name" value="Arp2/3 complex 21 kDa subunit ARPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR016315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="184" mass="21487" checksum="3E186595CD08D3C4" modified="2017-01-18" version="1">MPAYHSTFQVDPQNDRFVGNFVLLPLNTTYRGPAYSANSDYDIIDECLDLFRANSFFKNFEIKSXSDRVLIYGILYINDCLNHVKTNMSYNEAMKQLTNLSLDSNNFKIPGDIGFPLNNIYNIPTDNRQNVELLRQYLQQFRQELAMRLLKRCYADPSNPGQLIDTPSKFWLGFTRRKFMNKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH86</accession>
    <name>A0A1E5RH86_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Pre-mRNA-splicing factor 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1068</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH86">
        <property type="interactions" value="1363"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH86"/>
    <dbReference type="InParanoid" id="A0A1E5RH86"/>
    <dbReference type="OrthoDB" id="1931567at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097157">
        <property type="term" value="F:pre-mRNA intronic binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030623">
        <property type="term" value="F:U5 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000393">
        <property type="term" value="P:spliceosomal conformational changes to generate catalytic conformation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08056">
        <property type="entry name" value="MPN_PRP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13838">
        <property type="entry name" value="RNase_H_like_Prp8_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.230:FF:000001">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000002">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1570.40:FF:000001">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1570.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.40">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012591">
        <property type="entry name" value="PRO8NT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012592">
        <property type="entry name" value="PROCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012984">
        <property type="entry name" value="PROCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027652">
        <property type="entry name" value="PRP8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021983">
        <property type="entry name" value="PRP8_domainIV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043173">
        <property type="entry name" value="Prp8_domainIV_fingers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043172">
        <property type="entry name" value="Prp8_domainIV_palm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019581">
        <property type="entry name" value="Prp8_U5-snRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042516">
        <property type="entry name" value="Prp8_U5-snRNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019580">
        <property type="entry name" value="Prp8_U6-snRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019582">
        <property type="entry name" value="RRM_spliceosomal_PrP8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11140">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR PRP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11140:SF0">
        <property type="entry name" value="PRE-MRNA-PROCESSING-SPLICING FACTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08082">
        <property type="entry name" value="PRO8NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08083">
        <property type="entry name" value="PROCN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08084">
        <property type="entry name" value="PROCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12134">
        <property type="entry name" value="PRP8_domainIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10598">
        <property type="entry name" value="RRM_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10597">
        <property type="entry name" value="U5_2-snRNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10596">
        <property type="entry name" value="U6-snRNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="MPN" evidence="9">
        <location>
            <begin position="2219"/>
            <end position="2347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="135"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="2126"/>
            <end position="2153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="29"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="135"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="2450" mass="284110" checksum="17A2B31FFF101187" modified="2017-01-18" version="1">MNSELLDFASEDEDELLPPPPPPPPGFESDIENEXXVEETDFFQNLPPPPPPPPLADFEEHLDGSSTGETSLPPPPPPPGFDVDELLPCTTSEXVINSNSHNDIQYSNEIKQAHSNLSSRNIQAPDELEKEMPLRKKTKKNKXSLRKAPAKNTKVSSHKVDMPKEHLRKIMNDHIDMAGKRFXNDKNAHLSALRYMPHAVLKLLENMPQPWEETKEVRVLYHINGAITFXNETPRVIEPVYTAQWASMWIAMRREKRDLRHFKRLRFPTFDDDEPPLLYDESLDSLSVPDPMILELDEEDDERVKDWLFASKPLIADPQRVNGSSYRKWTLDASTMANLHRLATPLLDQVHDKNYYYLFDRKSFLTAKAINNPLVGGPKFEPLFPNEEHEESEDYTEFNSLNRLIFRNKVRTEYKIAYPHLFNMRPRNVEICDSGTHVSCLIESDELASTFGFQESLNNFTXHEAKKHKTAFEKEDFAPSFETFPAFRPILDNAPIVAPHTDDAIELYHAPHPFNKKHNKMIRAQDAALIKHWYLQHPDEQDYPLKVRVSYQRLLKXYTMNVLKTSTKHSSSDKKLKLLKNLRSTRYFQQTKLDWVEAGLQVVRQGHSMLNLLIHKRGITYLHLDYNFNLKPTKTLTTKERKKSRFGHAFHLIREILKIVKLLVDVQVQYRLGQSDAFQLADGIYYILNHLGHLTGIYRYKYKVMRQIRACKDLKHVVYYRFNKVIGKGPGCGFWQPAWRVWLFFLRGIVPILERWLSNMLARQFEGRSNEVAKTATKQRVDAYYDLELRASVMNDILDMIPQNLRQSKAKTILQHLSEAWRCWKANVPWNVPGMPDPIRKIIEKYIKAKADGWVSLAYQNRDKIKRGEKVEKTAVKKNLGRLTRLWMRNEQEQQEEMQEHGPDITPEEATTIFSTMVDWFEATKFQPIPFPPLNYKHDNKIFALALENLKEKYAVKSRLNASEREEVALIEEAFDNPHETLNRVKKFLLTQRVFKPVYLDMVDNFQEIQPVFSVDPLEKITDAYLDQYLWFEADKRNLFPNWIKPSDSEIPPLLVYKWCQGINNLHNVWDTSESQTNVLLQTTLENLPNKIDLILLNRLLRLIMDPNMADYITAKNNVTINFKDMSFVKKLGLLQGLQFASFVYQYYGMMCDLLILSPERALELAGPADNPNQFMEFADVQTEVKDPIRLYCRYFDKIFIFFRFEEFQSEDLIKDYLMENPDPNFENIVGYNNRKCWPRDSRMRLLRSDVNLGRAVFWEIESRLPVSITSVKWEKTLSSVYSKNNPNLLLTMCGFEIRITPKVRIPGGNSSGESVWDLIDNKTKERTAKAFLRVSEDEVKKFESKIAVLLMSAGSPTFTKVASKWNLALVALFTYFREAIASTEXLLDALVKAETKVQTKVKLGLNSKMPTRFPPVVFYSPKELGGLGMLSASHILIPTSDLTWSKQTDTGITHFRAGMSHDDEKMIPTIFRYITTWENEFLDSQRVWSDYSVKRREALEENRRIAFEELEGYWDRGIPRIGTLFQKNRHTLAYDKGFRVRSEFKKFSSDQSGHFWWTNPNHDGKLWNLNNYRSDVIQALGGIETILEHTLFKGTGFTSWEGLFWEKAAGFEDSMQMKRLTNAQRTGLSQIPNRRFTLWWSPTINRANVYVGFLVQLDLTGIFLHGKIPTLKISLIQIFRAHLWQKIHESVVFDICQILDDQMDLLQIEEVNKEVVHPRKSYKMNSSAADVTLNSTNTWKVSQPSLLHETNDKYEKKNSISKFWIDVQLRYGDYDSHDISRYVRAKFLDYTTDNVSMYPSSTGVMIGIDLAYNMFDAYGHWFENFKPVIHNSMKTIMRANPSLYVLRERVRKGLQLYQSQTQEPFLNSSNYAELFNDDVKFFVDDTNVYRVSVHSTYEGNISTKPINGCVFILNPKSGELFLKIIHTSVWVGQSRLSQLAKWKTAEEVTALIRSLPKEEQPKQLIVTRKPMMDPLEVHMLDFPNVGIRPTELKLPFSAAMSIDKLSXIVLKANEPQMVLFNMYDDWLESISSYTAFSRLVLLLRALKANEESAKNILFQDPTIRIEENHLWPSFSDDQWIEVETSMRDLILDEYSAKYNVNIAALTQTEIKDLILGQNIKAPSAKRQQMLDLQESKRILEEEKQNILNENSDSATVLKTKSVNAQGEEIVVVTSANYETQTFSSKDEWRARAVSNSLLSLRLKNIFVSQADFVEDKNVYVLPHNILXKFIEISDSKAQISXFIYGKPASNDQPHIKEIQTVVVVPQLGNTHYVSMSRHLPKHPYLKDLELLGWMHTQSDELKFMSSTEVTTHAETFSNVDTECIDLCIASSPGSVAISAYSLLDEGYLWGINNKNLQDSDPEGFEPAFSEYAQVLLSDRIMGNFMVPDNDVWNYAFMGSEFEKDIKYSLKIDVPIEYYHELHRPFHFIQFKNFLGSEDDLEADQEDVLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHA3</accession>
    <name>A0A1E5RHA3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pre-rRNA-processing protein TSR2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1214</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TSR2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHA3"/>
    <dbReference type="FunCoup" id="A0A1E5RHA3">
        <property type="interactions" value="197"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHA3"/>
    <dbReference type="InParanoid" id="A0A1E5RHA3"/>
    <dbReference type="OrthoDB" id="263560at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019398">
        <property type="entry name" value="Pre-rRNA_process_TSR2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21250">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN TSR2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10273">
        <property type="entry name" value="WGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="142"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="160"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ86267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="208" mass="23707" checksum="85D65EF3889A26A2" modified="2017-01-18" version="1">MNFSNIDASAFCEAISPKTNLLFDDEKQQANFELGVSMMIYKWDALDVAVSNNWGGPDSAEKRDWISTIVIELFQNNKYVDIQMIEETLLYAMVDEFETNVEDDSSLPISAGILEIWKNCAQNDYTIPQALYQAWTEKQKTQTGPRKVEIVNDPLNGTDSSEESDQDYDDDESMMVDQEGDLEMDQESNNEPIIDDDGFELVQPKSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI07</accession>
    <name>A0A1E5RI07_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA-binding protein SRO9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1630</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI07"/>
    <dbReference type="FunCoup" id="A0A1E5RI07">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI07"/>
    <dbReference type="InParanoid" id="A0A1E5RI07"/>
    <dbReference type="OrthoDB" id="340227at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045180">
        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF132">
        <property type="entry name" value="LA-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="4">
        <location>
            <begin position="344"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="88"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="482"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="118"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="148"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="220"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="237"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="324"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ86532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="512" mass="55962" checksum="1F9F473DC9F91174" modified="2017-01-18" version="1">MSTIEQVQEPVVSAVEQTEQAPVAQIVDEKSSTNETATPGDVVEEAAEPEKTFVPAPVPVASPWKVVDVSSATTGGKKVPSVAEAVEELKSNNENKSRNNQLLRSSSKDRWVPLHANITVSTKQPNSSPRQGSGKPKSNGTPSGPQRATKKKRTTNKRKQTGENETNGEELASDSISESSIPEDSKASSASASSKKPVHGNGVSKRATNKSKPFKKTQDEASIGSGESSETAESSNVEENATQVSETEGADSAERKPFSKRQNGYSYSNGNNNNRPFSRNGNGPRRSTQPRHHHNTGDGSSRPGYYNNNNGYRKHHNNYGYGQFPSQNFGNGTESQSHFKAHHPPSPYFAIKKVAKQLQYYFGEQNLTKDAYLKNQFSSKNGFIPLSLISKFYRVVNLSYGGDPNIILGALHQILSSSPSESNVEVAVLKENLENDNVLSKYVIRSNKWEQFLGEESQDIDYDLEKTVEAADLASFEVEAPVFETSTEEHNENNIDEPQAHAQSAKVIEQSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI58</accession>
    <name>A0A1E5RI58_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Peptide transporter PTR2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1212</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RI58">
        <property type="interactions" value="1105"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RI58"/>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="360"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="405"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="436"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="478"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="516"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="547"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="640" mass="71651" checksum="D8E3B5352AD1A4FA" modified="2017-01-18" version="1">MMSNIEEKNNIKNDAIELDSSSYDAVDVSYSTGSDVEXGEFREPTEEESKTLRRTTGTVPWYCYLILTIEFAERASYYCVSDRLSNFIELPLPAGSKTGKVMNSSQNDGALAMSLSRANSTSNALSFFAYCFPLVTSYLADMYFKRMTMLKAGVVIGIVSHIIFIIAAIPSVIARPTASYGVLFTGIFTLAWCTACIKPCLLPTLLQQYPHRSDVVTKTKKGELVIWDREQTLQKQTIWFYFAINLGAFVSLGCGYAARRIGYWLAFLIPMLIFLILPVVIFFLEGRIDDPKPLGRSILNDIFKILKVALEGNFIKRIKNKTFWEYPHPEYLQETQPERLGWRKSKPGWYASHLVNDTKVTLSACVIFLYYVMFNINDVGLGALINNLSASMTTNGVPNDVINNFNPLAIIFFVPFLDLCFYPLCRKMGIHWKPTWKIFTGFMIAALAMMVGAILQSWVYKHSPCGDQASSCEEHAPISLWTTAIIYALTGLAECFANTSAYTIAFLRAPNNMKSFVMALFLFQTALSSIVGIIISPAMVDPKLVRVFIGITVVGAAFAFGFLFQFWNLDKTMEKERLERLAFENAEAAEAAELLAQQEGEKATTSYELMDGDNQHELTSRLNQPLSPVLSAVSAQALKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI82</accession>
    <name>A0A1E5RI82_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Superoxide dismutase 1 copper chaperone</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1357</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RI82">
        <property type="interactions" value="287"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI82"/>
    <dbReference type="InParanoid" id="A0A1E5RI82"/>
    <dbReference type="OrthoDB" id="666972at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006801">
        <property type="term" value="P:superoxide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="332" mass="36030" checksum="EB81BE2F2345FB3B" modified="2017-01-18" version="1">MPENRQTSSGVPIPQFEATYLTSLECESCCKXLINTLTTIPNTVVSQITFPYFPSLGYPKQARPAVVSTEQHDKGLFSIISNIQPSKLVKTVDTDLGKDTILRGSSKSSAVAILEDFSSSAAQSSYANNSDSVLNYANFTGVKEHAIRGLVRIVSLPTASMLDGKTVAGSAGSKIFCDLTLSNVLHKDYVFEVYEFGDLRLLENAXSNLSSVGDITKSFGKRIISIPITAQNSEIHDKKTSGDVARFDISSFVDINNGTKXKTDMEMWELIGRSLVIRPSDAAGSDFNDNSEIIAGVIARSAGAWENTKQVCACSGKTVWEERRDALEKNIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RJ74</accession>
    <name>A0A1E5RJ74_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Eisosome protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1347</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EIS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RJ74">
        <property type="interactions" value="87"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RJ74"/>
    <dbReference type="InParanoid" id="A0A1E5RJ74"/>
    <dbReference type="OrthoDB" id="3972697at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024527">
        <property type="entry name" value="Eisosome1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28298">
        <property type="entry name" value="EISOSOME PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28298:SF1">
        <property type="entry name" value="EISOSOME PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12757">
        <property type="entry name" value="Eisosome1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="558"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="773"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="465"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="611"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="10"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="793"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="860"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="876"/>
            <end position="889"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ86613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="889" mass="99166" checksum="76B6C7DB82DA292A" modified="2017-01-18" version="1">MSLVSALDRSTXSANTTNTATNXNISTTTTTTNTHTYKPPNVYQVDGKPLSKEALYKAKLKYGFYQSPAIQQSTGFADTTKSSNKAASLAAENYYASVNTTGTHEEQQHLSKEAQYKRLLVGPDASKIAAKTSASATTTSPSSSSSXSSSFSTAAAAATSSTKSSSDNGRTLFDTQTLQKVAKQAIQKNNTHKAKKAKTHTEKVELPTKISTGNINLSKVLVGAERNAHKRIAQASGVDDKGVAVGNKNLKFNYYVKKENQKTEEFKHQVLGQGVPEWYAQANKAAATAVDQFHEQLDYKLDAKGEKYAHDAAEKSGPILNKERQQEEARRDLYLNANAHSHVLALARESVLKQMNAMEFEAVSKRDPSLVLFSNEEFNKAAVEYVTKKYDANAQERSTLKNKINLGGGLLLTQKELDEIAYKIVSPLLKEIDSRTVEQRRKDAEIASKQKYNKNETKLYKLNKQEQLERERARTAFEKQHVKEEETLNKELVQIEKTGELINIPDLLQQIKTHNNEINKILEDDEDLEQKIKNFEQDIATHTASSHQLSKDIKLKKSAKDKALTXTPSNESDTEESLNKYDYQIYDNEALMKIAEQAKERAFVIIQQKNLQNHKHDLELNEKVKQTLQQEIDIKLEKLKSLEDLGTKSKEKYNMLNKLKEENHVNIVSRGLYIRSTFGKDLDALIAQERENEIRARKEASANEDNLYNDNLSEAXVGAVQTNKVXENEVPATXTTDLVQDDVLTEEIPAEDPEAEGAEHDDAYKLAKVESELGADLDPATDETVPASLVDEKLDDEVPEPVEEPVEESVEEPVEEPVEEPVEEXVEEPVXXPVSAPVQKTVVETTQEEGVSAAPAPAEVSDDDVAEEEESSINNDHQEKRQSLFKEIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN56</accession>
    <name>A0A1E5RN56_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nuclear pore complex protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g888</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as a component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
            <location evidence="7">Nuclear pore complex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the nucleoporin Nup84/Nup107 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN56"/>
    <dbReference type="FunCoup" id="A0A1E5RN56">
        <property type="interactions" value="570"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN56"/>
    <dbReference type="InParanoid" id="A0A1E5RN56"/>
    <dbReference type="OrthoDB" id="3973031at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3450.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007252">
        <property type="entry name" value="Nup84/Nup107"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13003:SF2">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13003">
        <property type="entry name" value="NUP107-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04121">
        <property type="entry name" value="Nup84_Nup100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="5 7" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0811">Translocation</keyword>
    <keyword evidence="1 7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ88319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="728" mass="82248" checksum="45716790B592A107" modified="2017-01-18" version="1">MNSVLSLKQTLVSFSNVLKDYQFSQLSEQPTDIEKVVQEFKKISNKEAERSLLKPTDAENWELETKLWHLIEILVTFRSASQDGDFFSDDMTIYDYNSNVVYWKKFLQNDKALYEIWLVMVWIQENLKVPARPDNMSGLKWPNSSVSGEMSSCDVDCPLRENSILDKADAANDEIAFKYIFNLLLCGKVEQAKKECELTENIALGMILCGMDEYCDPVLDSQIEQANEALITSPSGCEKKVLWRRAVYSLGQNPSLGKYERAIYQYLAGTVPNDSQDDITEYNWDTYLLLYLNKLWNICIENYMNSIGRFPKDQTVNEIPESKTSIQTILNTVALKSGSECEHPTRVLMAAIIMDDVNSVIKSSVNELVKSVQALEQQSLFEKEPFLLRLLTHVSIVCDTYSPHTVDNSDKTKLISSYITILNMYELYDAIPIYASFLDHDSLLQCYSFILTTMNNRDLKKSQLSICTALQLPTSNILKKLAQTVFDSTAKYYETSVGEETVIKSAVDSSDHKLINAIDWLIEGHLYSEALDMIVAVSRSFLLCGKTEALSALMQEIDVIALIKELKLSALAECDENKSASSVNLKTNEVSEYNNLVEGLLLFKKWGSSSLLLNNQSNLPSLVGQFNEFSAHFYKLITNFLSELVGDDATLYQIRALYVPYLVMELHKSLMSASEALQITSFAHEAMNLSCVVADESNQIYLLFQSSGKLKEFLTLVAQSAIKVGIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN83</accession>
    <name>A0A1E5RN83_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Nucleoporin NUP53</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g927</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN83"/>
    <dbReference type="FunCoup" id="A0A1E5RN83">
        <property type="interactions" value="176"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN83"/>
    <dbReference type="InParanoid" id="A0A1E5RN83"/>
    <dbReference type="OrthoDB" id="1733656at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044613">
        <property type="term" value="C:nuclear pore central transport channel"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044615">
        <property type="term" value="C:nuclear pore nuclear basket"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12721">
        <property type="entry name" value="RRM_Nup53p_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007846">
        <property type="entry name" value="RRM_NUP35_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21527">
        <property type="entry name" value="NUCLEOPORIN NUP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21527:SF6">
        <property type="entry name" value="NUCLEOPORIN NUP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05172">
        <property type="entry name" value="RRM_Nup35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51472">
        <property type="entry name" value="RRM_NUP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6 8" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RRM Nup35-type" evidence="10">
        <location>
            <begin position="294"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="8"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ88340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="533" mass="58085" checksum="5BEFE661F7A20CF8" modified="2017-01-18" version="1">MNGPTGFFGSSNNKVNPTTSSGLFSQNSAPSLTLPLQQQQQQQQQQQQQPLAPSLFNPSSTQTLGGSTLSFNSGMPNNFQQNQPLLQQQTPLYSTLQPQPIPTSQSSISRQEIKTTPAWFADNIKRTTPQTIVRRSNKETNDDQDSFSGTAAPATAKDAFGTISFGTKKFNMCNITKNVTSTFNSVKLTDSDQQGSPDKNNLNFHLDSNDAPPAKSIFDLQREDEFSNHAMDNGVVEIAGSSNTTDESNKSPAKKMGIFKSKNIFDKSKRQDSTSEAVQIIKPLKAESHATTVDAQGYAVLVFGYPESISNQVIAHFSKFGKVMEDFEVCREEPAFGLSRFKNPSETAVDRTKYPIFTGNGWVKITYNTETSALRALKENGTLYGGVLLGCVPYSKDSVEGLTNCKIEKYQDSFSVSAPAPPSGKSFPNSNLNLSAKVQAQPVSTKAQKESVDAKNEPLFANARKLNVTSGANIFTHNNSGSLYNNTNNGKSLKHGGWYNTLESVMTQDVASSKHPNGLVAKLSNWLFGWGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN91</accession>
    <name>A0A1E5RN91_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g955</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN91"/>
    <dbReference type="STRING" id="56408.A0A1E5RN91"/>
    <dbReference type="InParanoid" id="A0A1E5RN91"/>
    <dbReference type="OrthoDB" id="10261433at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43094">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43094:SF1">
        <property type="entry name" value="AMINOTRANSFERASE CLASS-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ88344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="472" mass="51784" checksum="5BE8164FCF1920B8" modified="2017-01-18" version="1">MTQDTAQETYVFLNTINKKLPQVLGGKGINITIEKDGKVYDDIIDAMTGAAVGALGWGDEDVPKIVAKAVENSTYTFPFYVGNQASEDLAKFYIDHSPKGAFSSALWCCSGSEANENAMKIIKQYWLERGQPKKNRFISRETSYHGFSLGALSISENNRATNFLDILIDQKNICLKMPACYPYRLQKEGQTTEEYVQELMDALEKIILDADPATVASVTIETLPGSTMGTPPPPPGYLPGIRRLCDKYDILMHLDEVMCGTGRANPNGGLNCWENYMEPGQFPDIQTVGKTLGSGYVTIAGILVGPKIKEAYEKGSGMIIGSHTYSSHAFNCAVALGVQQKIMDNGLTKNIFEKGNSLGKKLSDALIPADNIVGDVRGIGGFWSVEFVKDKKTKAMFPKELHVAYRFQDICFENGLTVMGMQGCYSNKTGEGDIALIAPAFIVTDEDIDEIAKRFVKSVDELSKALKEEGEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNB4</accession>
    <name>A0A1E5RNB4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Putative tyrosine-protein phosphatase OCA1</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g695</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Putative tyrosine-protein phosphatase required for protection against superoxide stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNB4"/>
    <dbReference type="FunCoup" id="A0A1E5RNB4">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNB4"/>
    <dbReference type="InParanoid" id="A0A1E5RNB4"/>
    <dbReference type="OrthoDB" id="6375174at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14531">
        <property type="entry name" value="PFA-DSP_Oca1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000035">
        <property type="entry name" value="Tyrosine phosphatase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020428">
        <property type="entry name" value="PFA-DSPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004861">
        <property type="entry name" value="Siw14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF8">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE OCA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03162">
        <property type="entry name" value="Y_phosphatase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01911">
        <property type="entry name" value="PFDSPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="12">
        <location>
            <begin position="53"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="17"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ88379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="219" mass="25081" checksum="04027EB843BBB903" modified="2017-01-18" version="1">MTTKQIHSPQTSILNDPFKEQQHHEQEQEAVNRKEEQLVHIIPSTEKIVPPLNFCPVERYLYRSGQPSPVNFPFLSSLNLRTIIWLNNEDPQDSLLEFCDYNDIFLQFAAINPDGGEDDNPWDGLNEKSISNALKTLVNKENYPLLVCCGMGRHRTGTVIGCLRRIMKWNLASCSEEYRRFTGSRGGRILVELLIEGFDTSKIEINEATAPEFMLNALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNH9</accession>
    <name>A0A1E5RNH9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Inheritance of peroxisomes protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g948</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNH9"/>
    <dbReference type="FunCoup" id="A0A1E5RNH9">
        <property type="interactions" value="126"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNH9"/>
    <dbReference type="InParanoid" id="A0A1E5RNH9"/>
    <dbReference type="OrthoDB" id="4045067at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ88445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="871" mass="100943" checksum="3FAC5C725A3737C7" modified="2017-01-18" version="1">MSTDLGLGILNRDELNQTLFKDVARYKSARQKNHDRALKKSLNTSGNLSLLLSSVHEGVLQQPQGYRHRGSDEHTVKFSDSSPTVVNEEVFKNETHSTNAYSTLFHTIPFWQITKNSYHSQKNVRNNGRYKKSYLPQYPFQSAHKYDKNINNLLDQILELKPFGDDQFVDELQYTIISSSLFSAQSENEYARLLSLPIKKSVLDFRKVNNTKVCNQSNHSEHTITKYGYIFTREKKLVLKKLNSSYLNTHLFVYSMLKNIYISQQKNYTKFTNKLVICLVIQAYLNYQQESFRNRYIKYSALLSVKSTIRSLEKINILLQKYENLYKELLVHKSMDNDNCSRPNTENTDENEGELLQQLQMLLKATIYPIYQKLIKAVELFVPLINQNILQKHCKLFSVDLVCIHSYYKFVTLDQDLDYQVSCFRNLVKLFLICMLSMQTLQVASSTKEDKNLAQSNIINHEIDVFFQNMFTFIEPCTIMNDFDKLHFLKKQNLSVEKFITPFYETLLMKRFWFFGSSAASRDKDSFANASRPPSPAFDYTQILLHEQNQQHRNQSVQKMTFIMNQLSNQLLTANKIKFSTQKFKTKIASEIQNLQDLWTAISLAPVEDTDLHSPYPDSSDVAQKMFQFPAGSVKNTSGSNNAHMHKKHIRQPSNTGFHLNILDIDSDVANTALNKMHRNVSLQPLKSCVIDGMQKESVNTKKTSLSNSEQKQTLEARKLTTPIDFVRVDDELDIDDIGSYFNNEELENTFLLKPPAINKFKTLSDEELRKKLDEKIHCLAVENKMHKKSMLKLRKEQRMHGKTDDAALAKQKSFNCAGRHPYQLGKNGKTNLKYSQSTPYDLNIAERPDMFKEESIPALYELNKLLNNEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNV0</accession>
    <name>A0A1E5RNV0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein BNI4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g582</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNV0"/>
    <dbReference type="FunCoup" id="A0A1E5RNV0">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNV0"/>
    <dbReference type="InParanoid" id="A0A1E5RNV0"/>
    <dbReference type="OrthoDB" id="5563016at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12751:SF18">
        <property type="entry name" value="PHOSPHATASE AND ACTIN REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12751">
        <property type="entry name" value="PHOSPHATASE AND ACTIN REGULATOR PHACTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="513"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="547"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="767"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="806"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="881"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="295"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="384"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="439"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="551"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="576"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="885"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="929"/>
            <end position="939"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1021" mass="114407" checksum="23A73F4F5B342872" modified="2017-01-18" version="1">MDSHENRKGVADNSPNPAQFNETNFYRSSNNLNFRDHAKNFRNSLFLSEDVHVEDLNFDKDGNLVQPDAHGASNATYFESQNDHQFKDSQTRKIGDKSSKTTDPSNPFLNTERKFEQNDYYGSDFDEDYGNSYSHVNTDNEVETQAIHFGLSPSLSSLAGVLKNNVIDIPKVDTMDKICEDEEEEEEEKEEEKEKEKDGRNRELNQETANKENKEVPRVRQHQLPQQHSSDTEYKRFEGYVPNATTNKSTVSRSNSTASTSASKQSRSQRNVEPEAYTKYKNAAPTLQKHHSTRSNRTSSITSNHLSNDAASTTNSKGKKKSIFSIFKKKQNISLDPLPVSETFSGKASLLKSNRKSSDSGRNVANDENGSSKFLGSWKKLNRSRTSITDDIPSKNTLQVKKNRSHSVASTVNTESSADSPVKRSDIGQRKPTPLDFNKTINENGHSKTEQTNAIQQRKQTPLNFELTFSKDQKIDDFVDNETISTPKMGGEDLFPKFLDSNEIESIKSLEKNRLSFSSKNGSKRRSLNDSISINAQNEGMYVEEAGDSFPISTPDLTKSPASSILRGGRFDDGTNRSSYNFSESTKSNRNSFAFSDGNRSNRNSAALSDGNKSNRNSVLFSETATSTLDNPVTTLQPLPPLKENYVTNDAAKNPLNNNTVPAREQEAIKMNNRDPSMESIEKLVNDMSMSENDTAFLKDEPSGSFFNTDFEDSEYVSEIMEFASIIDFGGDLDLDLDLNPGIGSSPNVLGLHKLPGHTEQVYKEPNFKSEETEHTSPNQAAVINPEKRVPSVDLTQDENVYENEAQKLVATPSPERGRFQASPRESLSPTSHAYIPKNTAEPTGARPLSMSFKGLHHSNQLNQSSSYESDIRAPLDAATVAPHQPEESFKEPLDAEYTQPQFPSRWDDENEYGLSADSSKEFSFETDPPSQLSTTSDGHWSGYPAEKADVYKPSGNRKLHFSSKIILYETYAEDEYDRRPELATCNQLTPQLAMMIRDELNELKSSMEVHPSSEQYTHFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNV3</accession>
    <name>A0A1E5RNV3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SEC14 homolog 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g584</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(out)</text>
            <dbReference type="Rhea" id="RHEA:38691"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:38692"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Microsome</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNV3"/>
    <dbReference type="FunCoup" id="A0A1E5RNV3">
        <property type="interactions" value="140"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNV3"/>
    <dbReference type="InParanoid" id="A0A1E5RNV3"/>
    <dbReference type="OrthoDB" id="75724at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000013">
        <property type="entry name" value="Phosphatidylinositol transfer protein PDR16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052578">
        <property type="entry name" value="PI_Transfer_CRAL-TRIO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45824">
        <property type="entry name" value="GH16843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45824:SF29">
        <property type="entry name" value="GH16843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2" id="KW-0492">Microsome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="5">
        <location>
            <begin position="137"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00083195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ88559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="349" mass="40662" checksum="F9EE093BD621ABDF" modified="2017-01-18" version="1">MFRRKKEAKDTVDTSKLIKIDLPIENLPASIKTPGLPPLTKEQEAFYEKVLHYFLQEENQLLPTTEKDCHNKEGPKEPLNDFEKAWLTKECLLRYCRATKWVFKDCIDRIVLSVAWRREFGISNFGEENGDKLTAADVEKENESGKQVVLGYEKDGRPILYLKPGRQNTKTSHTQVQHLVFMLERVIDFMPSGQDSLALLIDFKDHDDVPKVKENSRIPPIGTAKEVLHILQTHYPERLGRAMLTNIPWLGWTFLKILHPFIDPLTRSKIIFDKPFVDYVDKDELDVAYGGYLDFKYNHKVYWPPFLAMAEKKRCHYLTRFEKFGKSIGLSEIDLRGTDDELQYPVEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP24</accession>
    <name>A0A1E5RP24_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">mRNA-decapping enzyme subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g721</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nudix hydrolase family. DCP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP24"/>
    <dbReference type="FunCoup" id="A0A1E5RP24">
        <property type="interactions" value="349"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP24"/>
    <dbReference type="InParanoid" id="A0A1E5RP24"/>
    <dbReference type="OrthoDB" id="3972814at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140933">
        <property type="term" value="F:5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03672">
        <property type="entry name" value="NUDIX_Dcp2p_Nudt20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000045">
        <property type="entry name" value="mRNA-decapping enzyme 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1050">
        <property type="entry name" value="Dcp2, box A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007722">
        <property type="entry name" value="DCP2_BoxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036189">
        <property type="entry name" value="DCP2_BoxA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044099">
        <property type="entry name" value="Dcp2_NUDIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23114">
        <property type="entry name" value="M7GPPPN-MRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23114:SF17">
        <property type="entry name" value="M7GPPPN-MRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05026">
        <property type="entry name" value="DCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01125">
        <property type="entry name" value="DCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140586">
        <property type="entry name" value="Dcp2 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="11">
        <location>
            <begin position="106"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="876"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1078"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="896"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="1104"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1127"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1171"/>
            <end position="1181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ88627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1181" mass="132345" checksum="CE3C221D7B416959" modified="2017-01-18" version="1">MSLPLRSPFEDIKSLDRAMEDLLVRFVINCPEEDLSSEERVMFQIEEASWFYLDYVRMANINTQESLPKLKIKKFATKMIEICPLIWKWDDETISQALQKFSLYKKTIPVRGAAIFNETLNKILLVQGIESKSWSFPRGKISKDESDVDCCIRECLEETGFDLTNYINENEYLERNIGGKNYKIYLVKGVPKDTVFEPRAKFEIEDIRWIDFRKIGGYATTSNGNQSSYDSRRNQRYYLVNSMWKAIYVWVKKQKQLMKSGDKLEMKQNAERELKMILGLIKPEEANEQEVVQENGNINSQSFVPNKETTTADPGRDLLNILQSAVKKSSGPDLIGSENNTNSNLLPTINGQPSQIPLFPIDHPAMLHLNPPHLMRNGPVPPMMSPYAYNTPFAPINAVLPDHVQQQLSKNMINNGVPGNMPIMTPDGMVMSNGNAQMAASHSTMISNNQPVTIQPETGAKHLLSILKKPSNSNANDQQIFNNNNNNNNKAIEEKMLLKAMNKPQIDEIDKDLKRVNINGQHIQNGDPQTSDAQDLLNLIKKPIVNETKDGHSISMQEESKDNDLFYKKYHQKPDIADNKEFFQLLNRNKSNVTAPSADDLGISASNGSTQHKKIVHKEEDFESFDEFESSDENGNDEFFDATTTSTEHLSSPSNVIKSPLNANSSIKRNANDFFKNNNHVEEENSEDDLDSLVDENEEFATSTFPKIDLSEDQVSKRRILYNMNEQQDIKGNVEKISEIHGSPQLQQQQAPVAKPKFKILKRGEELPQPAPKAEEKVDIKEKTVTNASNTFEKVKENQESDEKNLAISKKDNVDLNEKFGNALIDSTTGKNHEIIGNANKAETQPFEEEKAKGKANEIIEEATENLQVLQMGKVHPHTAASPINKSKNSEEGDFSCQNDASNQNTAHAQESLDLLHLLNNNVQKSQINNDQGHTHAVKNKVQANAFDSNIPAQMKPPFTNDNLHGFSSPMHNMMPQNMYSPASPHVQPNQMMSNSANLLQMLKAGHNKPHADSKTAPTEPAGTMSLPQNMNGASFGPYQNQPIQPFGIPPHAASPLNPPFPMAGPFGFNAPSPAQHAFYGNNNFSGRDPMQPQHHPFSQQPAMFPPQQTPNAHQPPPFAHYPPHIDSPGPVNNNVPSSNVPDSTMLLNMLQNNNANSNGTRNINGNMNMDGKDGHKNSIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVB3</accession>
    <name>A0A1E5RVB3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Zinc-regulated transporter 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g327</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVB3"/>
    <dbReference type="FunCoup" id="A0A1E5RVB3">
        <property type="interactions" value="513"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVB3"/>
    <dbReference type="InParanoid" id="A0A1E5RVB3"/>
    <dbReference type="OrthoDB" id="448280at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004698">
        <property type="entry name" value="Zn/Fe_permease_fun/pln"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00820">
        <property type="entry name" value="zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF32">
        <property type="entry name" value="ZINC-REGULATED TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="312"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="201"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="216"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ90779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="403" mass="44587" checksum="C342BBEB664BF96E" modified="2017-01-18" version="1">MNSTVTQIAQIIAKRDDDDDCPTQSEYNGMLGLRVLSIFMVLISSSIGAFLPILTQRYSKNGVFSGETTKKVLRVAFFIARNFGSGVIFATAFIHLLDSGYEALTSPCLGGAFQEYPMAYAICMLSIFVIYLMEIISRHYVDRYVSRNGGPSQPSHTHGNFDFLMKDNSEEDHHSHANDTSIGAVHIHDDAADVENATDAHYHNHNNMDDEKDLENINNTTNGIATSSESDAESSLYDDDMSQRDIMNQIFAIFVLEFGIIFHSVWVGLTLGVAGEEFKTLFVVIVFHQMFEGLGLSTRIAECKWPKNKKHYPYLMALAYGLTTPITIAIGVGVRTSLNMANMTLSIVSGVADSISGGILLYNSFELLFGAFNEYPGNLKMKLLSYFTTCWGVGLMALLGKWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVF6</accession>
    <name>A0A1E5RVF6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sulfate permease 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g382</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVF6">
        <property type="interactions" value="509"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVF6"/>
    <dbReference type="InParanoid" id="A0A1E5RVF6"/>
    <dbReference type="OrthoDB" id="288203at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008271">
        <property type="term" value="F:secondary active sulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018045">
        <property type="entry name" value="S04_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00815">
        <property type="entry name" value="sulP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01130">
        <property type="entry name" value="SLC26A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="295"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="337"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="462"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="517"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="543"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="7">
        <location>
            <begin position="686"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="595"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="595"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ90758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="875" mass="97707" checksum="74A5C43840D1CC76" modified="2017-01-18" version="1">MSNELDDLELEYYNHQVHNHHGDQASPLQEENHPMHQSVNEYFHIDQNTGNNDSEAEFSQLKSVKSGQYLHTNSEVKVPYYEEDVVGFKDYYNHKLRGFIGLRAAGQYLLSFFPILKWLPHYNVQWFVNDIVAGITVGCVLVPQSMSYAKIASLNPQYGLYSSFVGAFIYCCFATSKDVCIGPVAVMSLQTAKTISTVLAQLPADTSVTGAQIATVLSLYCGIIAIGFGILRLGFLVEFISITAVSGFMTGSALNILWGQFPGLMGYKNEVNTRGKTYEVIINSLKHLPDTNINAVFGLVPLFLLFAIKYFCTNLAPKLIKSNKRLNDRQKFIWNKVFFYTNTLRNAVVIIVFTLISWGAWRHNKKDDIDRLGTVPKGFKDVGLMTIPDGVSSLVAKELPASVIVLVLEHISISKAFGRVNGYRVVPDQELVAIGATNLIGTFFNAYPATGSFSRSALKAKCNVSTPLSGIFTGSCVILALYCLTQAFYFIPNATLCAVIIHAVADLMASYKTTWNFWLTNPLDLVGFLVTVLITVFDSIDHGIYFAMCWSAAVLFFKVAFPSGQFLGRVKIAEVCNPNVDFQSSVVVEENGSXXSNSFSDSSKSFSKGSSKVNLDDKXSKMNNTNTNKAKKSNSKAGYMEYDGDYDDDSVELSKTKLKFYTKWVPFNHKYTRELNGDVQVLPPPPGVIVYRPSESWTYLNCSKLYDAVFNNAVANTKKGHIITEKKWNEPGEFKTPFFVKYFQKNKNENSAIDNHYNDERPILKIVCFDFSTVSQIDTTAVQSLVDLRSALNKYADRQVEFHFCGIISPWVRRALINSGFGTVNEEFANESLIVGHTSYHVVKSDTSDLEYGSATGVNLPFFHIDIPDFAEWDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVR9</accession>
    <name>A0A1E5RVR9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Mitochondrial GTPase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g556</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVR9">
        <property type="interactions" value="764"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVR9"/>
    <dbReference type="InParanoid" id="A0A1E5RVR9"/>
    <dbReference type="OrthoDB" id="269151at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45782">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45782:SF4">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="4">
        <location>
            <begin position="173"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="199"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="199"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ90936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="435" mass="50145" checksum="2F7C726E8F4A70CE" modified="2017-01-18" version="1">MFTPRYTFPSYNILLNKFKGHQFKTLNKLSQLIPKTPLIIELRDLRIPLSSRNPALEFLLDHHKCRHKRLVIYTNGDYLKQDPKKYETVLQKLHELHTPQATNYKKLSDEAPLEQKQPVTTKSFNKKPEPPLPFMVLDCRNLNXVSKKLLPILQYKYDLLEAQQKGYLPLGYRILICGIPNVGKSTLINSLRTLYATRPSRTNDSTTTQMAKSNETDNDEAPGSNMSVKQFNGNNHYISNSLNKKGKANVCKTGDYSGVTTKVSEIIKISNYKNGIYLIDTPGISQPVKTLSTSKTLSMSLCNESKESWNVIDPFLKADYYLFLLNILGESGYVGGKDKEYKNNDIEHVLKTWAQGRKSLQHKNLNWKKKSTGDLQMYKDENLLAHKWLQYCHDVLKIRIPSYDVDFLNLNYELNQQLIEQDVKKLGHEFLQKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVZ9</accession>
    <name>A0A1E5RVZ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">glutathione-specific gamma-glutamylcyclotransferase</fullName>
            <ecNumber evidence="1">4.3.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g423</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.7" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVZ9">
        <property type="interactions" value="327"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVZ9"/>
    <dbReference type="InParanoid" id="A0A1E5RVZ9"/>
    <dbReference type="OrthoDB" id="1933483at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061928">
        <property type="term" value="F:glutathione specific gamma-glutamylcyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006840">
        <property type="entry name" value="ChaC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12192">
        <property type="entry name" value="CATION TRANSPORT PROTEIN CHAC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12192:SF2">
        <property type="entry name" value="GLUTATHIONE-SPECIFIC GAMMA-GLUTAMYLCYCLOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04752">
        <property type="entry name" value="ChaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ91036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="249" mass="28887" checksum="BDEFB79952D39379" modified="2017-01-18" version="1">MTQSGKGLWXVGYGSLIYKAPIHYQYRVPGLIHGFIRRFWQSSSDHRGTPKSPGRVSTIIPYEDIVSNPSILHDTKNYLHLPQDHELTRDDLTILATAYYIPPEKVEEVVEYLDEREQDGYSTHSIEVHLMPPLPMVDEQYMEVTRVLNKTLPMHEDHHRNMRFYSLQSTVYIGTLQNESFIGPEDINSTAKIIASNVGPSGPNYEYLKKLYDSIERDLVTFKGQYHLKHRDHYLIELMEKTEKLKLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RW86</accession>
    <name>A0A1E5RW86_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g424</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RW86"/>
    <dbReference type="InParanoid" id="A0A1E5RW86"/>
    <dbReference type="OrthoDB" id="407617at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF37">
        <property type="entry name" value="PQ-LOOP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="190"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ91035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="305" mass="33677" checksum="8DB4EB10DCA14C81" modified="2017-01-18" version="1">MISEKGSTIAATLGTVCWCVQLIPQIIFLYRKKDCTGFPPIFMFLWVICGIPFACYTLISHANIVIEIQPHLFMFFCSISYAQTLYYPPYGQKSMKRVLLHLIPIVLVDIAFECGFVIWLRPMYAKGTTAPGIVFGSIASALLAAGLVPPYWELAKRNGEVVGMSFIFLGIDSLGAYLSIISVILGNMDIMGIVLYSIVAALELGIFLSHFIWCCRFKWFGKKEKLLDQEKDAELYSGAEGDKESLATVANGEPLVIGKIEDDNYGTQDSASGACIERTQSRSSLKDNSVSVQVQDEAPSIRTNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZ60</accession>
    <name>A0A1E5RZ60_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g13</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RZ60"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="unsure residue" description="I or L" evidence="1">
        <location>
            <position position="190"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="1">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="1">
        <location>
            <position position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ92290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="505" mass="57741" checksum="6FF050F7A30CDF40" modified="2017-01-18" version="1">MSLKHHPDDESAILHEVQHVITVEDEQHQKVNIKLVCKSQHIQEIVEYITNRISDPYRLLLNVNESTEENLIIKCDSIVNHGKTKCNCPFLVLDYNLDTNYFELWKELGFSALFRSSGKPLRKYVHSKVSTMPEISGXNSGLSEFPSGQQNTQCNVSKYDYSAVMKRLQLGSYDFHLLIKYCGYGPLEILNNDYAMFLQKHIQQQITSGHEKLDQSQILKLWGVQTIGEKKIRELQFNKETSNLIFTNMKYKSNKLLYFGFKVIADKNGIIISTSFQFFEVLTLKLRAFDCTLQQDXTXQAVSDLNSFVQRRXQKSWSSYISEKKPIYASATIENVLIDVACKFVNKFAXXEEQLXLFRLLSKKXVKFVVNLAWNDLIISINKTSVLLFLTEYAKFYDFPKTEGLVFXGAESWIIPDTYTTNMLKXQEIPTAAAQPXKHLXXDPDXYDTSFANETCSNSSSSDLSTQQPMQTNSNDEFASVFVLDKQIASIPDTTSPLGENNIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZH6</accession>
    <name>A0A1E5RZH6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptide hydrolase</fullName>
            <ecNumber evidence="6">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g19</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZH6">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZH6"/>
    <dbReference type="InParanoid" id="A0A1E5RZH6"/>
    <dbReference type="OrthoDB" id="10013407at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02130">
        <property type="entry name" value="PA_ScAPY_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000093">
        <property type="entry name" value="Peptide hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045175">
        <property type="entry name" value="M28_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147:SF17">
        <property type="entry name" value="AMINOPEPTIDASE Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147">
        <property type="entry name" value="METALLOPEPTIDASE M28 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 6" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009028127" description="Peptide hydrolase" evidence="6">
        <location>
            <begin position="20"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="8">
        <location>
            <begin position="192"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="9">
        <location>
            <begin position="309"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="32"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ92284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="563" mass="62204" checksum="3CD3405D928B95B5" modified="2017-01-18" version="1">MQVKSLMACAVAGSTLTSALLIPQFDQAEHPFQLNENSNSQVEQQLQQVEQQVAINTNDIVEKIEKIENQVDFNSWLHRPNWMKKTVDSEKLQEMITVEELNNTAAELYQVANASTHEYGHPTRIIGSPGHWATIDWILSKFDKHRDYYDVSLQEFPAVTGKVKSFNLTYTDGTNVKSAKPFALTPGVKNFTGKVLEVPNLGCKLIDYESVIPKKGKAIALIERGECSFGEKAKLAGKAGFHGAIIYDNEIGNTTISGTLGTPNKHNVATIGVNKIVGGLLKTAIELSLNDFYLNFGLDAYVEKIKTKNVIVETKHGDENNIVALGAHTDSVETGPGLNDDGSGTISLLTVAEHLVKFKVQNKVRFLFVSAEEEGLIGSSYYASHLSKKENSKIRLFMDYDMMASPNYQYQVYDANNIDHPNGSENLKNLYIDYYTSHGLNYSLIPFDGRSDYVGFLKADPYPIPAGGIATGAEGRNTDNGEVLDICYHSLCDDXTNLNFDAFLTNTKLIAHSVATYGKSLEDFPKRNIPCHHGGKNKTMSATTKEVESNKPFFKYSGSNLVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZN3</accession>
    <name>A0A1E5RZN3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g131</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RZN3"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ92296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="188" mass="21322" checksum="F8AC28945E26940A" modified="2017-01-18" version="1">MFGSITNHSSFKRMKNRQXFDNYQAVDGSRQGGLQYKDKIDHXGSVPEEDALAKKSNKIKASLVQCFHKAKKVFKGTSSEPTEEXCLARVANRRPPSPSIGTTNSSMFSLESHLFTVXRITLSHRIVSRILFNRPQTLALSTPRKKSDYACLIARKLARLKCLSEFKKRTPTRRFSRAHRLAVQGHQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZQ3</accession>
    <name>A0A1E5RZQ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosome biogenesis protein RLP7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g125</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the biogenesis of the 60S ribosomal subunit. May act as a specificity factor that binds precursor rRNAs and tethers the enzymes that carry out the early 5' to 3' exonucleolytic reactions that generate the mature rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZQ3">
        <property type="interactions" value="383"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZQ3"/>
    <dbReference type="InParanoid" id="A0A1E5RZQ3"/>
    <dbReference type="OrthoDB" id="28644at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_uL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018038">
        <property type="entry name" value="Ribosomal_uL30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_uL30_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524:SF26">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN RLP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00634">
        <property type="entry name" value="RIBOSOMAL_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="9">
        <location>
            <begin position="150"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="107"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ92279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="330" mass="37753" checksum="90CDC3EFFBE65BF7" modified="2017-01-18" version="1">MPADQPKQALNSTPEILLKKRRNADRTRLEKQELAQKKKELENKRKYAKHSDSRFVRAETIVATTLATSREKERIKRISKLEHKKSKNQISHLPSNVDFILKITEPATNSNEEDQDEDAVTLSQEDDEAXGENEGLVKEKVRYNGEDMLIFVIRVKGPTTAKIPKKPFTVLKLLRLLELNTGVFMKLTKTTFPLLKLVSPYVIIGQPSLNSIRSLIQKRSRILYQREGEAEPKEIILNDNNIVEEKLGGDNIICIEDIIHEISSLGENFNKCNFFLQPFRLNREVSGFSAVNKLMKLKLKEHYSKFLQNSNSSTAPVIEVDIDAFISKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZS5</accession>
    <name>A0A1E5RZS5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sister chromatid cohesion protein DCC1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g171</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DCC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZS5">
        <property type="interactions" value="428"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZS5"/>
    <dbReference type="InParanoid" id="A0A1E5RZS5"/>
    <dbReference type="OrthoDB" id="276989at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034088">
        <property type="term" value="P:maintenance of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019128">
        <property type="entry name" value="Dcc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13395:SF6">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN DCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13395">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN DCC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09724">
        <property type="entry name" value="Dcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ92451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="400" mass="45916" checksum="416B2ADF86621DC8" modified="2017-01-18" version="1">MKYKLCSKRLDQIDDGTGYKLLQLTPELLAHIEDDSITTKSLTLKPMDGPKSELVLCTDSSTFVIKQKXHSNCVLLMSRSDRAVXQDISAPEYNXNTSTDRGDDAEETMCTYEAYSQQCFELEPRKIEGKINIAQLPIYDGPDKPLLSTSYTLTISLSQFSESSPCSNKEFYDQWYASNGCMLAGQACVLPGRFIDKQLHLLLLCIVGAELDIANGLTMEQCKVAMGSNFQESNIEKTDLEQILKTILMKFSKNPVTTGENQDSQVYQLDLGKIAQWYGKKCLSKNCSNFQYIGIDDFMIKWKSSFPPFFSCDIDLEMLLGFYCKDLNHNKLCYLDDVMLPQDPKQRFSRLFSIQKSWLQQEIWPFVKDLNHQNLKPETFLIKYARRKRVKRDKFIITSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZY5</accession>
    <name>A0A1E5RZY5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Vacuolar protein sorting-associated protein 55</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g195</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OB-RGRP/VPS55 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZY5"/>
    <dbReference type="FunCoup" id="A0A1E5RZY5">
        <property type="interactions" value="528"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RZY5"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034424">
        <property type="term" value="C:Vps55/Vps68 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007262">
        <property type="entry name" value="Vps55/LEPROT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12050">
        <property type="entry name" value="LEPTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12050:SF0">
        <property type="entry name" value="RH04491P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04133">
        <property type="entry name" value="Vps55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ92369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="154" mass="16458" checksum="7682B08D069BFDD6" modified="2017-01-18" version="1">MAITISPLTKIIFLSGLLALGFLLVILSCALYSNYYPLFDLLLFLLSSVPRAMFPFGRGGSGTAGSSSFLGDSQGAGGALKDTGTYFTSWFLTLAIGLPVVMEHSNVINKSAMILSITGGGIIYSTIVLFGWFFSNGGSWLDEEDDDDYDGFGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S0G2</accession>
    <name>A0A1E5S0G2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">54S ribosomal protein L8, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g47</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the bacterial ribosomal protein bL17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5S0G2">
        <property type="interactions" value="658"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S0G2"/>
    <dbReference type="InParanoid" id="A0A1E5S0G2"/>
    <dbReference type="OrthoDB" id="275000at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1030.10">
        <property type="entry name" value="Ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000456">
        <property type="entry name" value="Ribosomal_bL17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036373">
        <property type="entry name" value="Ribosomal_bL17_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040894">
        <property type="entry name" value="Ribosomal_bL17m_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00059">
        <property type="entry name" value="L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL17M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413">
        <property type="entry name" value="RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18502">
        <property type="entry name" value="Mrpl_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01196">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64263">
        <property type="entry name" value="Prokaryotic ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein bL17m C-terminal fungi" evidence="5">
        <location>
            <begin position="173"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF18502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ92463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="293" mass="33845" checksum="2FB470A047ED6615" modified="2017-01-18" version="1">MKPSLASLGAKFKFSRTKSHRDAMLRNLTSQVFQHNAIISTITKCTAVQQWVDRCITFAKKRNSGSVNAKKFYTEKLLQKVFFKGENKNLLRGKLDLLATETYKNRKSGFTRIIKLEPRSFDKVPMGMVELVDLPIFNKVHTTKHVDIETSEGAEGAEGAEGTDNVVEEIAEVVNKGNMKLWMLMRTILKQEQEGVIXPLTLQNLSKMIALKNETQVNDFFSQDLLKMRKVIRENSNEVFNETEELEKLKLLQEKVFEFKEQQKSQKNKYFKGYNKLTVRPDWQKDVQIAANY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0P7</accession>
    <name>A0A1E5R0P7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Presequence translocated-associated motor subunit PAM17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4393</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the PAM complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the PAM17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0P7"/>
    <dbReference type="FunCoup" id="A0A1E5R0P7">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0P7"/>
    <dbReference type="InParanoid" id="A0A1E5R0P7"/>
    <dbReference type="OrthoDB" id="5970083at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013875">
        <property type="entry name" value="Pam17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28021">
        <property type="entry name" value="PRESEQUENCE TRANSLOCATED-ASSOCIATED MOTOR SUBUNIT PAM17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28021:SF1">
        <property type="entry name" value="PRESEQUENCE TRANSLOCATED-ASSOCIATED MOTOR SUBUNIT PAM17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08566">
        <property type="entry name" value="Pam17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ80474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="178" mass="20342" checksum="8A65769031662AD8" modified="2017-01-18" version="1">MLRVNPNIYKRAYTTASNIQKTKPSNVELTWSKFFQIRRQERKINIGSSVLSAFLTSNIAFAYVATIEIDPTQTIFGLDPSIVLIGGVMTSAAFGYLIGPSIFGKPIFQLRHKSVLEGYNLKNKIFLKHIIKNRVDASKQSFSNPPPDYYGEKIGSLKEYRRWLRDCHAYRIKASEFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0V1</accession>
    <name>A0A1E5R0V1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="16">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g4585</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:20630"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:47005"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="16"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0V1">
        <property type="interactions" value="1217"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0V1"/>
    <dbReference type="InParanoid" id="A0A1E5R0V1"/>
    <dbReference type="OrthoDB" id="445564at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000036">
        <property type="entry name" value="Serine/threonine protein phosphatase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013235">
        <property type="entry name" value="PPP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051134">
        <property type="entry name" value="PPP_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45668">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45668:SF5">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08321">
        <property type="entry name" value="PPP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033096">
        <property type="entry name" value="PPPtase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 15" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="15">
        <location>
            <begin position="9"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="15">
        <location>
            <begin position="77"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="17">
        <location>
            <begin position="323"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="14">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR033096-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ80529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="530" mass="60571" checksum="2701BE62D9C1F335" modified="2017-01-18" version="1">MTSGKEELALKHKDAGNKLFKDKNYHEAINEYSKAIEFDPTVSIFYSNRAFCNLKIENFTNSLEDCDKAVQLDNNNIKAYYRKGLSFIGLMQYKKAKTQFKIVLKVKPQDEGSKKALEMCEKIIFQNNFENALSGGDTLATANGVKDPKDRICNTVSLDSFDGNADLQSYNGVKLDFVHKSEKDTDLPHYEVTNMDQNFISFMVNDLFLKSKYLPKKYVAAILSHVDYLFRNEPTMVKLYNNNMDEDKTADDVMKRKITIVGDTHGQFYDVLNMLKKFGKVSDLDNHVYLFNGDFVDRGSWSCEVALLLYCLKIVYPTKIFINRGNHETDSMNQVYGFEDECKFKYSNRLFKMFSETFDSLPLCTLINDSYLVMHGGLTSDPETSLEEINKINRFKQPPREGPFMELLWSDPQEEPGFGPSQRGLGYRFGPDITEKFLNHNKLRKIFRSHEVRHNGVHFEQNGKLVTVFSAPNYCDVQGNQGGVVHXVPGKGTVGQHDNDDDDLIVELFQAVEHPNMKPMAYCKTGGLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1D0</accession>
    <name>A0A1E5R1D0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribosome assembly protein SQT1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4544</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1D0"/>
    <dbReference type="FunCoup" id="A0A1E5R1D0">
        <property type="interactions" value="636"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1D0"/>
    <dbReference type="InParanoid" id="A0A1E5R1D0"/>
    <dbReference type="OrthoDB" id="10261640at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051179">
        <property type="entry name" value="WD_repeat_multifunction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857:SF8">
        <property type="entry name" value="ANGIO-ASSOCIATED MIGRATORY CELL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857">
        <property type="entry name" value="MITOCHONDRIAL DIVISION PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="93"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ80373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="447" mass="49295" checksum="07AEBEECDAB5766B" modified="2017-01-18" version="1">MSEQPNAPNTGGDEPADYINLNEVDQEIVLNQDLPAPEDNEFEEEEEEEGEEGEEDDDESNKGGETSQPLEMAPENEQADIEIEMSNNSIGYFDKHTDSIFSISHHPRVPLVVTGGGDNKAHLWTSHSVPPKFATTIDHTESVIAAEFSKPLGKYLVTSDMNGLVKVSKSSKDGSKWKFLEEIQQVEEVVWLKTHPTVESMFAFGANDGSVWCYNIEDNNELTVLFSGFIHQQDCSGGDFLNVQEDGSAYLVTAAMDGTVVVWNCYTGDVVNKWASSDFKGLETPWVSLKTYSNNMCCLGANNGCLAVVNCTSGHVLHLTPCVIELKPEEDELGASIESIAWSPESHSIPLLCIGLVRGDVIVYDTASMRIRTEFQLPDSVTKILFRGTDIYISSIDGKVYKYDSRRFQEPSFTCVGHNMGVLDFCFAGEDKIITAGDEGVSLIYKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1M3</accession>
    <name>A0A1E5R1M3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mediator of RNA polymerase II transcription subunit 20</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Mediator complex subunit 20</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MED20</name>
        <name evidence="5" type="ORF">AWRI3579_g3950</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the Mediator complex subunit 20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1M3"/>
    <dbReference type="FunCoup" id="A0A1E5R1M3">
        <property type="interactions" value="255"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R1M3"/>
    <dbReference type="OrthoDB" id="10667814at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013921">
        <property type="entry name" value="Mediator_Med20"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08612">
        <property type="entry name" value="Med20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ80790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="236" mass="26986" checksum="D1608A847D39C572" modified="2017-01-18" version="1">MKHVVHIDTRDANTIVQLSDALINHSYSRSLQQWSFDSKTFRAPSKTEQTGADTNLVTEKTQYFLHFLSFNNQQEVLVNYKSVTSTSNSTQEKCHQNGIKLCVPGNFTKLLQNGCLFQENLQFGNDLIFNKILNSNKKWQQRQSVSGKQGHIYTLNKSTVKTINVYTSSNELKGCIVQIDSEDKETVNNVLVILNLFVEECLPKNSTYSLLDKEHETTDSGSEFQTIRDYIKFLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1P8</accession>
    <name>A0A1E5R1P8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">ER membrane protein complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g4357</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the EMC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1P8"/>
    <dbReference type="FunCoup" id="A0A1E5R1P8">
        <property type="interactions" value="754"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1P8"/>
    <dbReference type="InParanoid" id="A0A1E5R1P8"/>
    <dbReference type="OrthoDB" id="369569at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009445">
        <property type="entry name" value="TMEM85/Emc4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19315">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06417">
        <property type="entry name" value="EMC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017207">
        <property type="entry name" value="UCP017207_TM-p85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR017207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="185" mass="20690" checksum="08D86AC0317DA2CD" modified="2017-01-18" version="1">MSSQEVPQWVTNLTNKDFKPAVAINTERNSKTSRITSPPGFQNYSNKAVPPPARTLNDRRIEDMLIKKAWAIAMNPAKSIPMNLIMSYFSGSSLQIMSIITTFMMITGPIKSIITIKEAFKPVLTHESIKYDVKGAMAMFVVFQLLTMGIAVYKLNAMGLFPNTKSDWLAWEAPAQYVGGRSFFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1U4</accession>
    <name>A0A1E5R1U4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Putative mitochondrial carrier</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4361</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1U4"/>
    <dbReference type="InParanoid" id="A0A1E5R1U4"/>
    <dbReference type="OrthoDB" id="44467at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071913">
        <property type="term" value="F:citrate secondary active transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006843">
        <property type="term" value="P:mitochondrial citrate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049563">
        <property type="entry name" value="TXTP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788:SF5">
        <property type="entry name" value="AFR253WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788">
        <property type="entry name" value="SUCCINATE/FUMARATE MITOCHONDRIAL TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="385"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="306"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ80864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="422" mass="48122" checksum="02303ACCECE07C2E" modified="2017-01-18" version="1">MSAXTEQYLPSSDETTLIDVMQSQVLRASIVASLITQTLSHPFEYVKTGQQLSVGKQMYRMFDNKATLFFAGLASANISILGTNIIKFYTYPLTVNYLGKKTXEQGKSNSDHNQNLVATTAGVITGFFEGLLMVPFENIKNKQIYNAQICTXREQKALKIGKAEKDIRIQEDDAKSLKSQKDHAKRTPLNKAAKVAQEIEEISKTTPKQISYEDIAKENKEIALRVKNWKSKADYYYKHPSVKFYKILPEIWQVSSYRGFAQASYLTVFQHMTMVAIQFTALNTFLQMKKNSSNALFRLGEQYEVVQFMLPMVYGVATSAATVLITQPLDVVKTRMQSTKFAPFQTWIRDVPGRVTYPNTFSCFHQTFVEQNGMKAFYKGWMPRLFXLSVLNSGIGFGFYFHFQTAWENYLNGEPQEKPLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1X4</accession>
    <name>A0A1E5R1X4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein CMS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3934</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1X4"/>
    <dbReference type="FunCoup" id="A0A1E5R1X4">
        <property type="interactions" value="318"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1X4"/>
    <dbReference type="InParanoid" id="A0A1E5R1X4"/>
    <dbReference type="OrthoDB" id="1929311at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032704">
        <property type="entry name" value="Cms1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24030">
        <property type="entry name" value="PROTEIN CMSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24030:SF0">
        <property type="entry name" value="PROTEIN CMSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14617">
        <property type="entry name" value="CMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="311" mass="35611" checksum="2A2ADDFF5618A26B" modified="2017-01-18" version="1">MTSSVKTMSTSSDKTPPHIKNPKEYNYTMNADDLDDGLDYDFNSGVVSADEGETIALSDNENSYKRKLSHGDDEPNKKTISEDSANQAPLSKRQKKLQNSKFHNKKKEKMEFEKSQRLALSTLSGEDIAENLNKMIGLWYPDLSPLELEELYFKKTEFIPTTSYTEPRTAENFQDFVTKFTKAPKAIVFCSTNIRVADVFRNLGGSKQCLKLFSKNKLKEDLERLEELSQEKKKPHHNEIKYFISTPNRMEKIVKESPVFFQGKDKLDIIIDSSYLDPKTDTIFTSLDGKVLCSVLKKIIKEKSSVKILLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R239</accession>
    <name>A0A1E5R239_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Protein arginine N-methyltransferase SFM1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g4069</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R239"/>
    <dbReference type="FunCoup" id="A0A1E5R239">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R239"/>
    <dbReference type="OrthoDB" id="373498at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035241">
        <property type="term" value="F:protein-arginine omega-N monomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18090">
        <property type="entry name" value="Arginine_MT_Sfm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007364">
        <property type="entry name" value="SFM1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35517">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE SFM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35517:SF1">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE SFM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04252">
        <property type="entry name" value="SFM1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ80960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="217" mass="24924" checksum="FEA20B971F5084C7" modified="2017-01-18" version="1">MKYIVEHMEEGFSEWVILEYAKILSDVGKENLIICSLPSTTTSADIPAKLLDLGLKWTTKELDKINEEYPDFHYYTPESGKKICLLDPRAEEDLTCSDVEKFDCMVLGGILGNHPPRDRTAELKVKYPGLLVGRRLGDKQMSTDTAARTAFYILERKIEFENIKFIDYPEFRFNKNEAVEMPFRYIIDDKTGKPILPDGMLELIKEDSEQSLDDLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2E2</accession>
    <name>A0A1E5R2E2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribonuclease H2 subunit B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Ribonuclease HI subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3973</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2E2">
        <property type="interactions" value="93"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2E2"/>
    <dbReference type="InParanoid" id="A0A1E5R2E2"/>
    <dbReference type="OrthoDB" id="29098at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032299">
        <property type="term" value="C:ribonuclease H2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040456">
        <property type="entry name" value="RNase_H2_suB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019024">
        <property type="entry name" value="RNase_H2_suB_wHTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041195">
        <property type="entry name" value="Rnh202_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13383">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13383:SF11">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09468">
        <property type="entry name" value="RNase_H2-Ydr279"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17745">
        <property type="entry name" value="Ydr279_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rnh202 triple barrel" evidence="7">
        <location>
            <begin position="7"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Ribonuclease H2 subunit B wHTH" evidence="6">
        <location>
            <begin position="175"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="387" mass="43763" checksum="826A0AD613EE775B" modified="2017-01-18" version="1">MKSVLVLPIDVEDHQKRTLSVIQLPNPSNTECKTFIDLLLSSHTEHDTQKQTVYQLKGHHFSKGTSHNKFKDRENYYYKDSSIPLKSLIIHNSNDMHEGELCSNGEMKIAFKYDLTFSLIGALVAIAMKRAKHSAKSTAKTENDYYRPAETLVSKNQYGATQDKEEKEEEEEELFNGKVLPYVDLHESLIYNHDPQWEHISKELLVSKMRNVSDVIVESGEEYFKISKSKVVNFVLGKINKLVLEFPKSIETRFLSDINDKDVLTSGKKLYALNLLVSLLPLQVYDTVYSELGNDITKYNVYLKEKQEAKXKELRGDGEFDKKLLLQQAMTVGVIGGGSGSMSVAVKKTTSTMKKSVPAKRKPAFITSTTAGKPSRGAIDGFFKKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2N9</accession>
    <name>A0A1E5R2N9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2N9"/>
    <dbReference type="FunCoup" id="A0A1E5R2N9">
        <property type="interactions" value="227"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2N9"/>
    <dbReference type="InParanoid" id="A0A1E5R2N9"/>
    <dbReference type="OrthoDB" id="9992527at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08249">
        <property type="entry name" value="enoyl_reductase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047122">
        <property type="entry name" value="Trans-enoyl_RdTase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348">
        <property type="entry name" value="HYPOTHETICAL OXIDOREDUCTASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45348:SF2">
        <property type="entry name" value="ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE PROTEIN C2E1P3.01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="1">
        <location>
            <begin position="14"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="376" mass="40586" checksum="5002BC442A6010F9" modified="2017-01-18" version="1">MSIPKTMKAIVVEGDQAVLKNNVPVPQIGPKDLLVKVKAAAGNPTDWKHITFKIGPQGSIVGCDASGEVVEVGAEVDSSKFKKGDTVTAVVHGCSVLHPDNGAFAEYCRASSFTSVKIDNLTSSTAGTIPAGQRITNFESAAALPVSLYTALLTLAHNFQTNIDFEKVPSTPQHNFPLLVWGGATGFGQYIIQVAKLVNAYSKIIVVASKKHESLLRSFGADELFDYHDSDVLDQISEKYPDLVHLVDGVSNEETFKQTYQLASKNEQHKATVVNLMNFTEDKIPSNLRRNNVAIGLIFLYSISGEDIPFGPVIVPKDLDFAKLAAEVISKLQTPISQGKLKTIAIKNHKGLEAVPEILEGIKTGKNSGEKFVTTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4V3</accession>
    <name>A0A1E5R4V3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL23</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">60S ribosomal protein L25</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3759</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4V3"/>
    <dbReference type="FunCoup" id="A0A1E5R4V3">
        <property type="interactions" value="833"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4V3"/>
    <dbReference type="InParanoid" id="A0A1E5R4V3"/>
    <dbReference type="OrthoDB" id="1267328at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000035">
        <property type="entry name" value="60S ribosomal protein L23a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_A">
        <property type="entry name" value="Ribosomal_uL23_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_uL23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001014">
        <property type="entry name" value="Ribosomal_uL23_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005633">
        <property type="entry name" value="Ribosomal_uL23_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011118">
        <property type="entry name" value="PRK14548.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11620">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L23A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03939">
        <property type="entry name" value="Ribosomal_L23eN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00050">
        <property type="entry name" value="RIBOSOMAL_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8 11" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL23 N-terminal" evidence="10">
        <location>
            <begin position="5"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00075004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ81947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="140" mass="15410" checksum="99924B1174B123A8" modified="2017-01-18" version="1">MAPSTAAAKKAVVKGTNGKKSLKTRTSATFRLPNTLKLARSPKYASTVVPHYNRLDSYKVIVQPVTSESAMKKVEDGNILVFQVAMQANKYQIKKAVKDLYEVDVAKVNTLVRPNGTKKAYVRLTAEHDALDIASRIGYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4W5</accession>
    <name>A0A1E5R4W5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ATP synthase subunit beta</fullName>
            <ecNumber evidence="15">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g3534</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="15"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4W5"/>
    <dbReference type="FunCoup" id="A0A1E5R4W5">
        <property type="interactions" value="1236"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4W5"/>
    <dbReference type="InParanoid" id="A0A1E5R4W5"/>
    <dbReference type="OrthoDB" id="14523at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042776">
        <property type="term" value="P:proton motive force-driven mitochondrial ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18110">
        <property type="entry name" value="ATP-synt_F1_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18115">
        <property type="entry name" value="ATP-synt_F1_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01133">
        <property type="entry name" value="F1-ATPase_beta_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1140.10:FF:000001">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.170:FF:000004">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000026">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01347">
        <property type="entry name" value="ATP_synth_beta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005722">
        <property type="entry name" value="ATP_synth_F1_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050053">
        <property type="entry name" value="ATPase_alpha/beta_chains"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01039">
        <property type="entry name" value="atpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184">
        <property type="entry name" value="ATP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184:SF71">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039072">
        <property type="entry name" value="ATPase_subunit_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 15" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 15" id="KW-0139">CF(1)</keyword>
    <keyword evidence="7" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="178"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ81957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="507" mass="54274" checksum="959CFED4CC1C8224" modified="2017-01-18" version="1">MVIPRLYSASSRTALQAARKSLPFQSTSLRTLATAAAAHGKVRAVIGAVVDVQFEQGSLPAILNALEIKTPTGKLILEVAQHLGENTVRTIAMDGTEGLVRGEQVVDTGAPISVPVGRETLGRIINVVGEPIDERGPIVTKKKNPIHADPPSFAEQSTTAEVLETGIKVVDLLAPYARGGKIGLFGGAGVGKTVFIQELINNIAKAHGGFSVFTGVGERTREGNDLYREMKETGVINLEGESKVALVFGQMNEPPGARARVALTGLTIAEYFRDEEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGLLQERITTTKKGSVTSVQAVYVPADDLTDPAPATTFAHLDATTVLSRGISELGIYPAVDPLDSSSRLLDAGIIGQEHYDVATNVQQTLQAYKSLQDIIAILGMDELSEQDKLTVERARKIQRFLSQPFAVAEVFTGIPGRLVRLKDTISSFKGVLDGKYDHLPENAFYMVGGIEDVIAKAEKLLAESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R566</accession>
    <name>A0A1E5R566_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3666</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R566"/>
    <dbReference type="InParanoid" id="A0A1E5R566"/>
    <dbReference type="OrthoDB" id="1939598at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14705">
        <property type="entry name" value="bZIP_Zip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="2">
        <location>
            <begin position="450"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="330"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="209"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="341"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="391"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="3">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="3">
        <location>
            <position position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="523" mass="59411" checksum="FA083569CC3C9C23" modified="2017-01-18" version="1">MLGSMENARIDTHENIIENDKSPRFMVHDIEPRHYSAHQNLHSSAKLPSSLATGGSYXYTNRSQRQHHNSVTALSEEEEEQEEEQEQAQTNQGFAKKAAHEPKGNNQYSNNXQTQEEAEFEVEKDKDDEPSEYEDQDMHQNDQFLNQLQSLISSDQQFGTKLLQLLINKEAPSGLENLMNNRTVSTGKKEHLKDAPNASANNNLLAGLLSSASSNSPSPLDGSSTKKKTKKRKSSKRTDLLREMLQEKSETNKQQSYKPSSFEQNLTMRKSVSPIPSSIVTHEHIFPAQTANSHFPLFTRNRESTSPKSFFGLKGLKEXVSASIIPQRXPFASTKSPEQPNNNNTLRNNKSSGSSLKKVSSTSSPVTSFQPNDSFHSVSRNFLENSGPHASHNTYTKNTLSATSMNYKQTKIGDEKLDRSHFEELLPDTSATKISEKESKDKEDEALKLKRKRNMEASARFRLRKKLRYKENMTKYENLVSDVGVLKNRIIDLTKENEFWKKKLKCYYDLKNKELLENIKSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R586</accession>
    <name>A0A1E5R586_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3818</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R586">
        <property type="interactions" value="786"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R586"/>
    <dbReference type="InParanoid" id="A0A1E5R586"/>
    <dbReference type="OrthoDB" id="10253553at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037212">
        <property type="entry name" value="Med7/Med21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009244">
        <property type="entry name" value="Mediatior_Med7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044888">
        <property type="entry name" value="Mediatior_Med7_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21428">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21428:SF11">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05983">
        <property type="entry name" value="Med7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140718">
        <property type="entry name" value="Mediator hinge subcomplex-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="179"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ82075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="213" mass="24690" checksum="09DB2D1493E7E4A9" modified="2017-01-18" version="1">MSTNESNNVAALYPRPPVFYKYFTQQNIDKLEALKTKRKADQKXSHESGQTVNEANTEDLLSELEAANDDLQYLIPPSLPKSGQFRSFGNIWQIEDELPDLTKMGIQQLYKDSDDYSDKINNLKTLLKTLLLNFLELAGLLSVDPSKYEVKLEHIRVILINIHHLLNDYRPHQTRESLIMLLEDQLAFKKMEIREIKRMCNEVTGQLKELSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5A2</accession>
    <name>A0A1E5R5A2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein CRT10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3702</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5A2">
        <property type="interactions" value="83"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5A2"/>
    <dbReference type="InParanoid" id="A0A1E5R5A2"/>
    <dbReference type="OrthoDB" id="4068815at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014839">
        <property type="entry name" value="Crt10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08728">
        <property type="entry name" value="CRT10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1100" mass="125736" checksum="51182359A21891BA" modified="2017-01-18" version="1">MNNGSDQLARRNAEENDATNQEQETTNDENFQTFSPFNAEASQNDLPNEDVXTKAKEENVANIYPKNVVNQERYRKMRFFDYCMGYSDNLPDDKEAIDFDADVEANQSGKYPRTNLFFSGYLDIDILKFDFALKLLKNPRNMAQDVGPHFFVVTQIKHDPFENSYKRIFVDVPEFHTDDLKRRILEKEEAAEILPSLKVRKLASNVSMPHWKSGDTFPLGKHPSYKNNLAVMYKGYFISIALKGPATILVSKPEYGYSKEKILQTPDFFRRRKIEPYGLIEDEYLFSRYSRGLPWAPHSKALTLINYMKXCQLFGKAKLFICMGSPNFKTSPEDLYAAQKNGSCFHSNGCMVFDLEALINDAFSASDNTIKKKANSRKLEATVASLETAEYPTESEYVHPEDFQPDIIFETTDSVWSCDTYSDEDFDVVVLGTNEHMVRVYYASTALTQTGKFCKKFYKSFAIPIYHNVPTLQLSKTLLPTGNLLLTVGAVDGSVYTIELDINDEKQYVQDFLSKLKSAGGSKTDVLNAFLIRSAVCKGKYTLLDMQNLRNPVWTITPLDGKTDFVHADSWKSLTNSELPQFHAEKELACIQTEYLLGRGIDPQILDSQNYNVGALLTTIAVPTMALSSKFWCNKSGGSEYAKDVFNEMIQFETAHQNISPNEAKLPSMIFARNDLGPRLITVSGYEMYLEDCYNDYLVDDIEENERSEIKTVPCPQXLRAVXNKYSSKLXKPQEVDKSIITDSTSPKEKRPFLQANTLHNAYFLPTFRRWPEEIDGFSQDDMMNLQKFFHAADGLLGSQTFILVKDYFLEVFLHVIQDTPLFSIVESFLNSVKSVDILNFDKLKELLFVFKRFEDEYLHKDPMTWVEKILEKARILKTPPLTLEFITKDVPRTIAEEKDLFVECQRSKLGRLYGYELTSLESMETKLANNTYFLVTTESAIYLLYGHPLLVKAYTPESIFPLAHKTFTDHDTLQHSNRLSIVVHIKELCAVAVASQIGLVSIMRLVEYKGVKSFRQECILGLEFLTKFDETVEPYADNLNYSNLPVRVRGSDHELQSKGTNVAFPMATIIGMTYEMLSESVCMVYIYSEVGSFQYELRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5F4</accession>
    <name>A0A1E5R5F4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Transcription elongation factor SPT4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3619</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">The SPT4-SPT5 complex mediates both activation and inhibition of transcription elongation, and plays a role in pre-mRNA processing. This complex seems to be important for the stability of the RNA polymerase II elongation machinery on the chromatin template but not for the inherent ability of this machinery to translocate down the gene.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the SPT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R5F4"/>
    <dbReference type="FunCoup" id="A0A1E5R5F4">
        <property type="interactions" value="448"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5F4"/>
    <dbReference type="InParanoid" id="A0A1E5R5F4"/>
    <dbReference type="OrthoDB" id="248751at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032044">
        <property type="term" value="C:DSIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140673">
        <property type="term" value="P:transcription elongation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07973">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.210:FF:000003">
        <property type="entry name" value="Transcription elongation factor SPT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009287">
        <property type="entry name" value="Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022800">
        <property type="entry name" value="Spt4/RpoE2_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038510">
        <property type="entry name" value="Spt4_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12882">
        <property type="entry name" value="SUPPRESSOR OF TY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12882:SF1">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06093">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025023">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01389">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="RNA polymerase subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0137">Centromere</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="12" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="Spt4/RpoE2 zinc finger" evidence="11">
        <location>
            <begin position="5"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR025023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ82131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="103" mass="11414" checksum="046322533051247D" modified="2017-01-18" version="1">MSSSERACMLCGLVQTEAEFVSKGCANCQSIFDKAGVDTIDCISPSFEGYVGMCKPNKSWCARWLRIDNFHQGLYAVKVDGRLPVDVVDLLPHYIPRDGSHVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5K0</accession>
    <name>A0A1E5R5K0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Alkylphosphocholine resistance protein LEM3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3822</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the CDC50/LEM3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R5K0"/>
    <dbReference type="FunCoup" id="A0A1E5R5K0">
        <property type="interactions" value="311"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5K0"/>
    <dbReference type="InParanoid" id="A0A1E5R5K0"/>
    <dbReference type="OrthoDB" id="340608at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005045">
        <property type="entry name" value="CDC50/LEM3_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926:SF20">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE ACCESSORY SUBUNIT LEM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03381">
        <property type="entry name" value="CDC50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015840">
        <property type="entry name" value="DUF284_TM_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="395"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="31"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ82148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="436" mass="49604" checksum="F0B0B5FCBBBDB33A" modified="2017-01-18" version="1">MTSQLASKGKNLGDFFKFKNGGFKLSSLNHFNNKNGSQEEVVPEEEEVDASEFEDDDEFEEKKDPHSRRPKNTPFVQQRIPSYNPIFTPRLTLSIYLILAGIFILFGGILLAMYQRTDQVILYYQECSSSAPQDDFGTIPTEYVDTHFHKTNKSYVFDAQWKYTPLDSSSEYMGDENGTCTLKFTTPYELPHPLYMSYLVKNFYPNHRRYALSFNEDQINGVKTSISDVKESTGISCKVLHQDDNGKQLYPCGLIANAMFNDSFDLTLTNTENSSNSYDLTNKGISWSTNPDRFKKTKLSYDQIAPPPYWEKKFPNGYNSTNVPDISTWEEFQNWMDNPALPVFSKLLGKSSEKKKLTSGTYELDIELNWPVSMYKGKKAFFLTHGSPVGGYNDFLGITYLIGGLICLAVAVLIIGMKLLSGRKTADPSLLSWYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5Q1</accession>
    <name>A0A1E5R5Q1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative transporter MCH4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3766</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5Q1">
        <property type="interactions" value="182"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R5Q1"/>
    <dbReference type="OrthoDB" id="6509908at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032218">
        <property type="term" value="P:riboflavin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17352">
        <property type="entry name" value="MFS_MCT_SLC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF295">
        <property type="entry name" value="TRANSPORTER MCH4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="380"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="408"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="445"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="477"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="4">
        <location>
            <begin position="98"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="5">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ81863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="507" mass="56163" checksum="54547909C65D0E3A" modified="2017-01-18" version="1">MTARENEYLYESTSAIMHGGSNATGYNKQKINEQRETEGFPLQTISHEEPWNSVXNVNTGKNKHEDQISTAFLDEHSTDDQRENVFYDASSFPDGGFKAYTVVFGSFMGLVPVFGLINSLGAIESYISHNQLSQVRPSTISWIFSIYLSVSFVSCVFAGAYFDRNGGLKPMLFGTLFYVGGIMAMANSYKVYHFILAFSLCCGLGTGICTTPLVSCVSTWFYHNRATATSLATIGGSIGGIVFPIMLRKLYEKVGFEWAIRILGFICLFCLLMAAIFAQERKDPNHKRKEFENKKELVSWYFTTCFNWRYFLEGKFLFATLGAALAENSLTSSATYLASYSLLRGNSETTSYALITVTNAVGILGRYIPAFLADKYVGTFNIVIITVSMAAFWNFVLWLPFGGNTACLWVYVCLYGFSAGSILSLTPVCIGAISKTSDFGKRYSTTYLAQAVITIPVIPISGVIIGDGNHIKGYNHFIVFSTMFMVAGALCYLTSRYLCVGTRLCKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7B0</accession>
    <name>A0A1E5R7B0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Elongator complex protein 3</fullName>
            <ecNumber evidence="15">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AWRI3579_g3313</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalytic tRNA acetyltransferase subunit of the elongator complex, which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>uridine(34) in tRNA + acetyl-CoA + S-adenosyl-L-methionine + H2O = 5-(carboxymethyl)uridine(34) in tRNA + 5'-deoxyadenosine + L-methionine + CoA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:61020"/>
            <dbReference type="Rhea" id="RHEA-COMP:10407"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74882"/>
            <dbReference type="EC" id="2.3.1.311"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:61021"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="15 16">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the ELP3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="15"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7B0"/>
    <dbReference type="FunCoup" id="A0A1E5R7B0">
        <property type="interactions" value="1074"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7B0"/>
    <dbReference type="InParanoid" id="A0A1E5R7B0"/>
    <dbReference type="OrthoDB" id="10265243at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106261">
        <property type="term" value="F:tRNA uridine(34) acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000003">
        <property type="entry name" value="Elongator complex protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034687">
        <property type="entry name" value="ELP3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056591">
        <property type="entry name" value="ELP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01211">
        <property type="entry name" value="ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF0">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23613">
        <property type="entry name" value="ELP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005669">
        <property type="entry name" value="Hist_AcTrfase_ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01086">
        <property type="entry name" value="elongater_protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00344">
        <property type="entry name" value="ELP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="13 15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11 16" id="KW-0408">Iron</keyword>
    <keyword evidence="12 15 16" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 15" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5 15" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Radical SAM core" evidence="18">
        <location>
            <begin position="92"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="17">
        <location>
            <begin position="406"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR005669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR005669-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OEJ82799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="561" mass="64166" checksum="C3A5096A516B411F" modified="2017-01-18" version="1">MARRGKNPKSTNYHKLAPEKERFIQCCSDITLELTNSLTNTSTNEINLNGLITNYSKKYKLKQQPRLTDIINSIPDQYKKYLLPKLKAKPVRTASGIAVVAVMCKPHRCPHIAYTGNICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYDPYEQARGRIEQLKQLGHSIDKVEYIVMGGTFMSLPKDYRENFIVNLHNALSGFNGTEIDEAIQYSQQSLTKCVGITIETRPDYCTHSHLDDMLKYGCTRLEIGVQSLYEDVARDTNRGHTVKSVCETFAVAKDAGYKIVSHMMPDLPNVGMERDIEQFKEYFENPDFRTDGLKIYPTLVIRGTGLYELWKTGLYKSYNANALIDLVARIMALVPPWTRIYRVQRDIPMPLVTSGVENGNLRELALARMKDFGTTCRDVRTREVGIQEVHHKVQPDQVELIRRDYYANGGWETFLSYEDPKQDILIGLLRLRKATKKYTYRKEFTSQRTSIVRELHVYGSVVPLHSRDPRKFQHQGFGTLLMEEAERIAREEHGSEKISVISGVGVRNYYAKLGYSVDGPYMSKTITPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7C6</accession>
    <name>A0A1E5R7C6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative glycosyltransferase HOC1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3485</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7C6"/>
    <dbReference type="FunCoup" id="A0A1E5R7C6">
        <property type="interactions" value="109"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7C6"/>
    <dbReference type="InParanoid" id="A0A1E5R7C6"/>
    <dbReference type="OrthoDB" id="411251at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.10">
        <property type="entry name" value="Mog1/PsbP, alpha/beta/alpha sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007681">
        <property type="entry name" value="Mog1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016123">
        <property type="entry name" value="Mog1/PsbP_a/b/a-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039367">
        <property type="entry name" value="Och1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834:SF11">
        <property type="entry name" value="GLYCOSYLTRANSFERASE HOC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04603">
        <property type="entry name" value="Mog1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55724">
        <property type="entry name" value="Mog1p/PsbP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="250"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ82796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="604" mass="69815" checksum="4A2F9E4ED5CC0411" modified="2017-01-18" version="1">MAQPMELVPQPLYGGAIEIMLPKGYLDVSQLREIPDNQEVFVNNNDMEGGNKNGMDTGKNGGNESIIVELMERVDVKDGESAIKEHCKEIVEINYSNKQLSGSFDNSFEVIDVQVVASNRLICKVKQKNVGKWENDKEQVQELDLYIGLIRLVEYDADALITLNVPISALSKDVEETNSSKYTNLKMAKRKTINFRKLIMFLMIPTAIIFFLGYRFLKFSNSSDLQSLLQNLPKEITDSISLANSRQKENEEMFKKFEDLTKDLMEVNKKQNQQLADQKLVLQNKMNELREPPETATLREKLTWVYGYELKSKFPAYIWQTWPYSQYDDRMDVSLKKFYDNWHNKNPGFVHELINDDIMNLLVNHFYKAIPEVIETFNILPTQMLKADFFRFLILYAKGGTFADMDTDPIQPIPNWIPENVSPKEVGIIVNIEVDAQTKDWQEKYPRRLQFGTSVIQCKPRHPVIREVIATIIETTLKKRHDGEARMNLKEDMNIVKWTGSGSFTEILMKYFNDYIQSGILSKVTWKDFHNLEMPRLVGDVLVFPQFSFNAPRSFDNSDERKPLYFVSHERMKSWNGLDNIKNKNLEQKQEAAADAAADAAAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7H0</accession>
    <name>A0A1E5R7H0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3205</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7H0"/>
    <dbReference type="InParanoid" id="A0A1E5R7H0"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="96"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="189" mass="20706" checksum="E4E210BF565A3E75" modified="2017-01-18" version="1">MENKEDSATAPIPAHLSEFVDILKNNKLRKSTSTVFNSADTEGGNASASPALLSFGSRNFSFSGHSPQQRRSSQSYAGSMTSSLYQFQTIISKKDIDNTKKDIKALQTEFQKFAKQLDEMTKTASNIGAVLENLSNNSKSEDDEMLLNLISMLWVLPHVRNSSTGAQPNDQTSIFETLGTPHVSNNAQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7K0</accession>
    <name>A0A1E5R7K0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R7K0"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="204"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="364" mass="40825" checksum="EC3733E6B2C10E87" modified="2017-01-18" version="1">MSSDQQEKLEMASKNNSSSKKSNLESLQKDGYDKKFVLPPIKNLFKDNDXIDYENKNVIGNGDSIATNDINIVTNTDSNTSTNNDAXGFVENSAPKLGTKLGTKLGTELLKSDSDMFSAIKXDAQIQXNGQKDLVDSGKNVVFDSLGPNFSSKIKLPSLNDFYYPPSTPNNVNSSTSHMKNTTIINNQNNFTLTPNRIMPPLRNNAYNPSNNHSNNNDNNNNNNNRSNNDNNDNNSNNNGNNNHHHHHNHNNNXQLYYYNYATMDMSHKELKPTNDMELNKMKNDSEIESRPISSRDSNNSQVIQPNTKFENMKLRLLNSRDLMNGLDSNSLNNPLNNPLNNPLNNPLNIPLNNPLNNTINNSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7V0</accession>
    <name>A0A1E5R7V0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3392</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7V0"/>
    <dbReference type="FunCoup" id="A0A1E5R7V0">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R7V0"/>
    <dbReference type="OrthoDB" id="2556122at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044695">
        <property type="term" value="C:Dsc E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045226">
        <property type="entry name" value="Dsc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025390">
        <property type="entry name" value="Dsc3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28049:SF1">
        <property type="entry name" value="DSC E3 UBIQUITIN LIGASE COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28049">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN YOR223W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13373">
        <property type="entry name" value="Dsc3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="DSC E3 ubiquitin ligase complex subunit 3 C-terminal" evidence="3">
        <location>
            <begin position="164"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="203"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ82990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="341" mass="38598" checksum="45B844D6D7932DDA" modified="2017-01-18" version="1">MGKCFLVIRFSSDFENDIKLDITKLPIHNITPAWLRRMCRDLRSSKHGNTHASTEDSEEAEFANRSVDLNKCRLKFIRNGRLLTGMHWNEFEMELRSAKESIAHTAEGQENDKYIYVQCLIGIPEEDDIDVQQELEEDRRSYTASAPSGEGQNGLGNETPQPVIGFDRLRSLGFSDEEIAMLRQQFQARMNVRNFAAGGGPGNRPNGAVNTNATNNGPEDDEYDEDQIFEDVTGEEQLGAHANTGEARLGQQTRPVDPREMEERWMETNALEDVSTDIANNITMWKYNSELLLGVSIGFLLGVFGLLLLIFFDKKTFNTRIQRAIFAGLTANFFFGIIRGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7V2</accession>
    <name>A0A1E5R7V2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3400</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7V2">
        <property type="interactions" value="380"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7V2"/>
    <dbReference type="InParanoid" id="A0A1E5R7V2"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF32">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="109"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="427"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="475"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="554"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="656"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="775"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="842"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="898"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="17"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="478"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="507"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="560"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="577"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="600"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="668"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="776"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="842"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="898"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="932"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="942"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="964"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="975"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="993"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="11">
        <location>
            <position position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ82974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1004" mass="111077" checksum="91912AAA33228105" modified="2017-01-18" version="1">MSAAGPATSTAMMQPKRYTYYGTGRSTTGAVSSSPAPVSSPVASSSSGNFSHQYYSPSQQQQQKQQNQPHSANPYSSKRHSVHAMAGGSATSLNSSSKPQRRQAVFGPYILGRTIGEGEFGKVKLAWSVNKKASDSNHYNINSNMGNLQMSNGVNSDDIPKQVAIKLIKMETIPKGSERETKIFREINALRLLTHPNIVQLEEVLQNKTHIGIVLEYASGGEFYKFIQERKRLKENQSCGLFAQLISGVHYMHSKGLVHRDLKLENLLLDKFENLIITDFGFVNEFDPGRNELMRTSCGSPCYAAPELVVSSRPYLARRADIWSCGIILFAMLAGYLPWDDDPTNPEGDDIAKLYMYITQTPLKFPEYIAPVPRDLLRRILVPNPKNRLSVRQIEQHQWLQGQKSFLSITPEEWDKILNQNKVYRPSQSKKKVVRPKSAASVSSNGEKRNSLVLDNSYNVQPLPPQESQIFATVSDVSGKEKDNEQKREYVDASLERLAEPNPSFTKDSEPRPTFFADKHDRPENVYTPVASTNRHRRTNSAASIVLQAVYDASESQNNELKETTKDSSIDTEPRNLHTSKNTGKSKFQTSSKPRKPRPTSYQPSMSRDTSNYTNPFTFPNKNMSQVLPEASLSEHDAMKDNAKREKQILEQKLKALKVNENSNSTSERSHGREPKNYDNGRKPNNSIIQEEDETEDISFPSKHILDLDQSDLKYNTARSNIFSAGDEKFESPAASKPRIVSDASSISSPIPFNKVKLDNLDAATAAATAAVAGLTGQNSSRQTSRNVSGASANEKAGRRVSYNAYHVNNDNNTPVSATNNYTQASYQQQQRQFSNTLHSVATPNATTMPGANPASSXTKDMKDKKTKRFSLLSFYSLYDNSKSSLDVEKAGPVGATANNSPAATYNPPNSATTAGSSRTMGSSSQKPATATKERNKDRRVSSVNTSGNTTHSSYNSLATPTKKHGDTYEKHGSRNTSGTESGGSNTPSTAKKVLDFFKRRSMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R803</accession>
    <name>A0A1E5R803_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein EFR3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3471</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EFR3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R803"/>
    <dbReference type="FunCoup" id="A0A1E5R803">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R803"/>
    <dbReference type="InParanoid" id="A0A1E5R803"/>
    <dbReference type="OrthoDB" id="19232at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039786">
        <property type="entry name" value="EFR3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049150">
        <property type="entry name" value="EFR3_HEAT-like_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47766">
        <property type="entry name" value="PROTEIN EFR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47766:SF1">
        <property type="entry name" value="PROTEIN EFR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21072">
        <property type="entry name" value="EFR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="665"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="786"/>
            <end position="808"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="665"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="713"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ83040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="843" mass="94617" checksum="F90E96EA2A1413AB" modified="2017-01-18" version="1">MALRNLFSTKHQRLVNQCYPTGKTTEKRAKSSETSYLIYYVNSRRTKLDKVSVYLLKRSMKDIHGRRIGNIAVTLELMGKIIVSCKENLSIFLSNFLKILLTIEDNKSINGDSSIVQGLIQVLENLCSIIDVNLITPNQLTSFEKFIALYFTTICGRSSHDSPQASNYNEYILKGCIAITGIKGIKFISSLRDQLFHGAYQSLKLFQQIHPHYLTPDLNTQISEDDALNKRLTLTRSITHRQLGLDDYADEKTQDLSIISLKKYFNTLETDILPISIRALIKVLIETPNKELFEFVVNGIPVQTRYIAIIIIVNAIVYSSSSKDAVVLLKLVSTLVYTEVSIVGLSVVDTIKNLLNFQLSKVEDKDIVAQCTVTIGDLNTRTFYKDQTFDVIQEFLRRLNTNESGNLPQSSGANSGEKQQRVKTVLIEDLNCLLSLVKAQNTECISFELFINLMDKTYPDMENLQNIFRMIQTSSVNTNSMLKFYQTLAIVNNRNPTSESSTMAQTLLGQSFQRFGPIALLAGLVFFQQPENCKPNVQYYQYHLQASKFLNLKDYEQQAIGHQKHSTVFTNESLLNFYIDEGANRYSAKGQQILRTIPSAAGDTSIRTVSRSTTDVESQKNGFQSKAPSYKAIVEGQINTEDKSEADIVDSLMNTTLSNMLKMTPLSSPSMHPLSSPKQRAVSNDVHPESKSLHSLSYKKPSVSQLRKYVKPSKTNSSLPNTGPLTNRASQSVKSKVTNITFLLSELNDQSSEIPIRDPDEEEVIGLENYGKSSAQTKLKRSSLKRMSNSSNFGSIKSNSPSVNMHTTANKHPAQIDEEISDAFVDAQNDITFDDTTRGKLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R835</accession>
    <name>A0A1E5R835_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">F-box protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R835">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R835"/>
    <dbReference type="InParanoid" id="A0A1E5R835"/>
    <dbReference type="OrthoDB" id="5351126at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="417"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="359"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="427"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="895" mass="103150" checksum="077576C2D3AEC205" modified="2017-01-18" version="1">MTTTHRSHHIDDVRILNTNYNKYYTSIYEKLKKHNPSKNKKESVYCDDLITEEQTNIKTEPNIIEGINDANVLNATKEDSKMTSMYQYTYRSLTSPMTYTINTPIEHIKCLKKRDLNGVIHETNNKSLKNRAKEYLKNKLDLSRSSTLLHSNISIQSLTSNNNNVHPLFFCRSTAPYKTIDGLPQELLTRIYDYLVDDRRALLRGLYVNKQFNKSCKIVLYKEITLYSTYRLAQLVTTLQENSYLRRYVEYIDLSQLKSGMELYQEYMIFMKMANENKMKNLTKKNGNSNININLNVENTEYLEQLQEDVSTQEEMFASWRDWRFRNDLLYSATRLKRQRTMSCSSLSLSTTEKRPRITRNSSTPGAITGTTRNKRNSISLPTTTQNAQNKNKKWKTNHNNNILLKSLLIFKMSSLSKNSKNDGSKKKICHSKNKRSKSMNKSINKPNGHLQTTTPIKNNLAQNNTSSTVSKDNDPIPRIQHPLSNKFLSKYCMNKDVPIGQLLHLIEYCSSNLRILKLNSLSLSEDFIISNTTFKGKSQKQNSTQSTKAKNGFTQQRKVAFVFDRNYASNRGLACSNGGVERKDLKIRRNNKKQEDLDKVEIDDLGPNSGKEVVYFSDTSYHTLNIEGNPKIKKVTTNDIFAKLADLKNLENLELENCIFINENIVTNLLLEXLPDINLHQVCPEKTLSHYAFQGSGMVQLKWALNGTSVEILIMILLTQLVHRTKDSTLEALFGINIHKPLFCISDKSKKXFGENDTNKPSVLXQYNGESLMATSDLIYLDFDTDHRVAFSVSVYETFENIKEHYSYQVVPASSNSSGSPVIALHCYLNKLHNSAIYLPTLRPXNTDINNHIINYMKKLYVQRVKYLRVLRLRDNIGYNMIQHPTNYSHADRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R889</accession>
    <name>A0A1E5R889_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Tyrosine-protein phosphatase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3370</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R889">
        <property type="interactions" value="148"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R889"/>
    <dbReference type="InParanoid" id="A0A1E5R889"/>
    <dbReference type="OrthoDB" id="6058203at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF561">
        <property type="entry name" value="PROTEIN TYROSINE PHOSPHATASE 36E, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="3">
        <location>
            <begin position="641"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="4">
        <location>
            <begin position="826"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="94"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="272"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="359"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="404"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="113"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="310"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="361"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="404"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="425"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ83125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="950" mass="107770" checksum="0582869D751BDEE7" modified="2017-01-18" version="1">MNNKMNINIPRNISRFSTTNKNNYIDLIKSDVYLNGKLVILDNENIDIFYEYDKETMNTENDILVLDMSTSMTNTSVTNKXVANTSVTNANTSVTNANGASVNNTTTSSSSSINKNDAQENKTLSNVSGNTKGXTPSEEQLDMVLSEIVSGCEKKYNITTMQLSFPYTLLKRSNFQLSKLIESTLYHNQQEQLLASIRDANIFIFYDNFNAANGNNSNSNACHCDISTYHIINKFITHLDATRNIHKNENDNNENNNNDYKVFYLNYQLEKHQPSSPGLESEYNENNXQDSNQEPLPKQPITTTKKDVSENVTNTPISTSPVQTTEISNMFANNKEIYEKKLAALNKHRAAMHKSRVIAAGTNSVPGSPTTINSPNGQSSSHKNKAHSRKLSLKLLIPNPDDQYTLNKVSKSTPSILGSPSAKDGSNGTKNFNGKGNMGNNGKDHYMNKKSYGSSTNRAGARHFLKKDSCYYSPSTLNKYFSFTRPITLSNTTNNKTISSSNTSNNVSDTQTQNKLTLPTWLDFLNDSNMNENDCLLQILERFQTLENLENDRLLKSMDVNREVEDVKTGNTSITASNNNNNTSVYQFNYETLNTNQFYHLNTPDCRSCKNLXFTDTNVPTHTSQASTPTYVFSQGIKYFNKNRYNNILPYEHTRVKLLADPQNNSTMALNSPSGIYFNANHIRIPKLNPNYQYIATQAPLPSTFDDFWQVCLAEKIEFIISLNSMDELNLKKWDQYWNNDYLHLFHIEQVYLNDSVENLSGCMLRVFEISPKLATSSDSSKNEKTETRKIVYHLQYSNWFDSASVVSTSIIEFMNIKNKLVNNPTAYIEFLKTCNSDPHAYSKSKALPQSSVKHYDTPLLVLCSAGCGRTGVFITIDFLLNLLNDHENPVRNIDFWKFPYDLIFIVVNELRRQRISMVQNLQQYIMCYQTIIEYLTHNYDAIANGSEKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA54</accession>
    <name>A0A1E5RA54_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial morphogenesis protein SLD7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2546</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle pole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SLD7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA54">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RA54"/>
    <dbReference type="OrthoDB" id="3973164at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041260">
        <property type="entry name" value="Sld7_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041564">
        <property type="entry name" value="Sld7_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18596">
        <property type="entry name" value="Sld7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18636">
        <property type="entry name" value="Sld7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sld7 N-terminal" evidence="11">
        <location>
            <begin position="15"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Sld7 C-terminal" evidence="10">
        <location>
            <begin position="176"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ83767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="314" mass="34768" checksum="BF584684116088DD" modified="2017-01-18" version="1">MASLQHNRTITLKYSSSIYLRDIQLWSESASSTSKPQTADSSINYSFVEYIDLKKLPLFASLSTSVRTTSRKPPVSTIITSSKQTAYFFIDKLITLQRCMLIRQKTVQNSYLLLFASQDISSLQDDRKDSQPSSAGIDNDISSFGLYAVQLDLEKLXKVIKSSKSTKDHAAKQSNARFDSMISECIASGLRLRGLDHVKNSGPGVTTFSTTVPGKNNSLYSAEXGGGYNKSSAISHFPSSSQSSTNRVLSQSFTDNSADTKLVYKSNTDAEYIKNLKEITFKATKFALSNTKLNDPFQECQETVETLLKLFTKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA55</accession>
    <name>A0A1E5RA55_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SIT4-associating protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3122</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAPS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA55">
        <property type="interactions" value="1082"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA55"/>
    <dbReference type="InParanoid" id="A0A1E5RA55"/>
    <dbReference type="OrthoDB" id="3972258at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007587">
        <property type="entry name" value="SAPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634:SF8">
        <property type="entry name" value="FIERY MOUNTAIN, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634">
        <property type="entry name" value="SIT4 YEAST -ASSOCIATING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04499">
        <property type="entry name" value="SAPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="152"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="377"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="445"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="804"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1019"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="152"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="171"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="196"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="244"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="393"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="807"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="822"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1020"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1050"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1104"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1125"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1161"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1212"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1232"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1274"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1299"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="4">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="4">
        <location>
            <position position="770"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="4">
        <location>
            <position position="1092"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ83774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1331" mass="150935" checksum="520A9084EE48BCFE" modified="2017-01-18" version="1">MASSFWARPKPNSSILSILHKAFIEVKEDSVSDVELQKQTKPSSHATGTAFTGXDGNDNVIVLDDKLLTKDQILITSSTNNLKYRPNLQVLDDFLKDEDLSRNLFLEPEDDPNETGLTLEDYFTNPLVLNQLLDYILDEXFCVEEKDQETELQNKKNKEHKDDNQDNEVEGASKKNAMKDLDESLLKRQKKQKYYDDDDDXEEEEEEEKKKEEENEDGQDDDNEKKHEDKLVSSETPQKKSFEDSDDSSDSSDDEEFGKFDNSLDDPELHDFLDESKSRHNGLEGDDDDENKNVELXEFFTKATVAAEILSIDLMEITASFFSHDILISKLWFMLDKYSTLSQNASSSFLKINERLLQYAEIGGLFGGNNGINDNSNSNNNFFQDALASSKDNDDDEDDDDDEEXDEGDNVDLFTKLNNGENVAKFNSTKNNXNNKDYDVDEDAAAAAADDDDDXDDDDDDDNDEEKLHKVVLETDEQVIDADEDDLMEGIDSNTIEITGNSTVLDRFLHIIIHREKLVDKFVKHIELAPLMDFLLKIISTDKPELPTYVTLFLKNHQLIEKLLFVLDGKNSATAQSTATDFLKALIAISANSNSEIASAIGPNELTRYLVSSKMIQKMGDLMLQGGLPLSNCVGIIIEIIRKNNSDYDFVQVMYTTLESNPPDMRDPIYLGHLVKFFAENLSKFNDILLSSNKVDFQTPFGLIEPLGVDRFKICELVAELLHCSNMALLNELGGENVVEERDVVRAKTVKDFLKLNFKIDTPEVLEFNDCVPSDATNKTITPTKTNSDDEHLNIKIENLNIKSEENSSEDNLEQQQDEDFETPKIDAETNDPNDKTIEGNDDDAVNHDESSAEALRMDPVVGDQLKIALQDSNIVDVILKMLFHFPWNNFLHNVVFDIVQQILNGPLKEGYNKFLIADLFKRSNITHFIVSSDEYCQRYEEEKNLRLGYMGHLTLIAEEVAKFAAYLDEMDIVMHDAAIENALKESEWVKYTDVVLSETREKYSTVFGDFNVDLYDVLGGEEDDGEDAEDISDDMVRGPNGTIHGNSLGDDDDYDEDDDDEYNATDNGNRSHLSGFKNDMDPSIDDDIPLDLRPYNSRYGXDHEDDEEQELDYSDEDNDEDEEARYGADKNEDLYGEDIEVTLNSHFHRMADSNGNSKTKNSDAKKKTLFDLLNEEEESSTSHNSKDHKSSMHTSSDDDDFDDDSHSPYSKQSYNSLNNYKPSLTSGTSSQEDEDDDDYIDPNDDGLSYAKPGHPLYSDSGKTFSEGTKMDLVDDEDDDVYEDAGDLIDDLDDDESGVSLSRQKTSDNMQWDQQEQNRIFGYKRLHNKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA63</accession>
    <name>A0A1E5RA63_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein GTS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2830</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA63">
        <property type="interactions" value="102"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA63"/>
    <dbReference type="InParanoid" id="A0A1E5RA63"/>
    <dbReference type="OrthoDB" id="3972898at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044518">
        <property type="entry name" value="ARF_GAP_AGD11/12/13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46220">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE-ACTIVATING PROTEIN AGD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46220:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE-ACTIVATING PROTEIN AGD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="3">
        <location>
            <begin position="14"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="156"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ83799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="388" mass="44992" checksum="BD0DD7694B90ACBA" modified="2017-01-18" version="1">MGFLKRKSNTNAFEKQLKKFVSSSVNKNKCVECLSPYPTYCSVTFHCLLCGRCANVHQKILPKSLVKSLSLERWNRDEIDELLDSGGNRENMKRYNDTDFPFPYDGDVDKTEVENFIRAKYILGKYTSHGIDENYTYEYLCSGNKNGGKYNGDDNDYNGYNDYDEDNYNNDRRSTYTSGSSSSRRNRENSGASRSNSRYSEVNDEXGPPLPNRPMNTGYAKPSLFDGESVTGLQTSVQTGVDSNFQSVQPREAVFDGSVDLQPYYNPNDGQVYVDKNQYQNFLTQQQLQQQQQQAQQQAQQVQQKAQVMNMYGNPGYYSSGVEISTNNPQYQQIMQQQQLQQQQQQQQQQQQQWFQQQQQLQQQQQQQQQQQQQQQQQQQPNFFNPYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAA4</accession>
    <name>A0A1E5RAA4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome c oxidase subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cytochrome c oxidase polypeptide VIa</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g2820</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome c oxidase subunit 6A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAA4"/>
    <dbReference type="FunCoup" id="A0A1E5RAA4">
        <property type="interactions" value="273"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAA4"/>
    <dbReference type="InParanoid" id="A0A1E5RAA4"/>
    <dbReference type="OrthoDB" id="5947505at2759"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.95.10:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 6A, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.95.10">
        <property type="entry name" value="Cytochrome c oxidase, subunit VIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001349">
        <property type="entry name" value="Cyt_c_oxidase_su6a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018507">
        <property type="entry name" value="Cyt_c_oxidase_su6a_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036418">
        <property type="entry name" value="Cyt_c_oxidase_su6a_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11504">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE VIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11504:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02046">
        <property type="entry name" value="COX6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000277">
        <property type="entry name" value="COX6A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81411">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit VIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01329">
        <property type="entry name" value="COX6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ83836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="122" mass="14207" checksum="88604E123E4E05E3" modified="2017-01-18" version="1">MFRQAFTQTVKRSVHTYKPDPAAAKAFRESIEQVEHHAAHTTALWKKISLFVAAPAVLAATVNTYFIEMDHAKHREHLAHVSDEDWPKEYEYQNIRTKPFFWGDGDKTLFWNPVINRHVSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAA7</accession>
    <name>A0A1E5RAA7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Hydrogen peroxide stress regulator 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2828</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAA7"/>
    <dbReference type="InParanoid" id="A0A1E5RAA7"/>
    <dbReference type="OrthoDB" id="3972862at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="B-cell CLL/lymphoma 6, member B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="726"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="12"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="7">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ83832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="750" mass="84673" checksum="851D18C7ADB57996" modified="2017-01-18" version="1">MGKVQESTTESSENRTNKERLVSKKVSENANIVNSEYXPQEVDQSFFEKSSLEKSNQFQTQCSYPQKXVSNKQQVHNLRHGDLNIDTLHETSQTSDLPNQALFPNAAPLGFIAANKINPYRNSFSETSGSTSYSTADDASVMGVADEIELGYNIYNAYHYNLYSDSNVPLIYQNQTEVIQNTEKEMKPVEANNGSTRNANSSVMPKNTSNFKKLFFSDQVLETNSITASIMSSSSILIDPRVQFQSQRFPXSLRTGTSSFSQNPKRNNQRNFTKHFNAVSSQADIENGQNKRLEQQYXIAQMQELYYNKVNTNFDYSEQHITNPVNSQRSNYQTTYVTGDEPNQCWYKNQVGNENKRRNFCEXSENKLNTVHNKNTDLKFLDDNGKYGNTNDNSLSFPSNEKTYEQISKTNDEDIAPDSYTKDNKAXFTAGKLMGEHDTCENSNVQNYENLEEGPLLRNYQNNPTGLYGPAATLDASESVPFFHSSTQYDVVSVDGPRDVQTGILEAKVFHKHLSYAMQRNNLEPNAQSYSASAPISIMQPYKNNKQAMLAAEGFALRNSNNVAHATTQPREPFTQHLSFPKNMQGFSSEQNYGRNTPVNQCVETRYSNEPNCGNQNFGVNPHSQSKLLPPISMASSLQNTDMGNNHGPDNIFGKKVNDMTASQYIKVQTKKSSSKNAKVRGRKPTNKGADSLMCPYCKDENACSFKRKEHLKRHISSVHIKKKPFQCPKCGKKFTRVDNMKNHVNICKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAC4</accession>
    <name>A0A1E5RAC4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2921</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RAC4"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="327" mass="37635" checksum="DEACCB39BD40BAD7" modified="2017-01-18" version="1">MDNEQVEDSRENSRENSRENSRENSNQDRKVNVTKDRKEEDNCXANKIHSSGKNSVVSTTPTRKMSTSPCSTTESASAKSLYSYQMDKMNNCDYYXSAEXKDKKITSQPSFLNXEDEIVYMSEMNGWNAGCNSSNEVTQTXLKNTFKKKKLQNKPEXERVTSGNNKFEDKENNDMNNRQVSLERDXEEXGKSPTPECPSXDYINNLEMYNYNNYNDNIDDSEQXGNGHENFHKKXNYNXYDILHANENSQSWQSFDEIREXNKGKLYKCNDDQDCINNTSKTKANKDNMXSMHQKNQSNGTRGKVLTDRIPTRGSPIXGNDDLEMQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAG0</accession>
    <name>A0A1E5RAG0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pre-mRNA-processing factor 17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2702</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAG0">
        <property type="interactions" value="888"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAG0"/>
    <dbReference type="InParanoid" id="A0A1E5RAG0"/>
    <dbReference type="OrthoDB" id="10257301at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000034">
        <property type="entry name" value="Pre-mRNA-processing factor 17, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032847">
        <property type="entry name" value="PRPF17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43979">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43979:SF1">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="2 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="140"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="185"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="269"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="371"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ83888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="435" mass="49594" checksum="D365294968C0854A" modified="2017-01-18" version="1">MGLISGYSSDSSNDSLSDTAEINSKRHLKPTKQESKLLKKQRKGNDPWAXWESSEEDEPLANQLISNQKESALSMLESAGLATLENEEISESDIQEFSQFFINKHYTENNITKPRVRXKDFVKETTKLKCYLPKKILHTYKGHVGGTNDIKYFPKTAHLFLSGGNDKTVKLWDMYHDRQLLREYNGGNHQAVRRLGFDSSGKKFLSSSYEKFVKLWDTEAGKVIHTFSLPAESTTIQFNPKIDDEFLVGLSNSEIKHFDIRDHRCVQTYDQHMSSVLDLEFFPDGSKFISTSEDKTVRIWNTGINITIRHISDTNQYAMPFVKIHPSHKVFLTQXMDSVARTYQMTPKYKQQKKKFVGHDCAGHGIGLSCSPDGRYLVSGDSKGKLMIWDWNTTKVLKSFQVDRTNKPVSIVAWSPQETSKVLCSGSVGKIQLYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB28</accession>
    <name>A0A1E5RB28_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">J-type co-chaperone JAC1, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2605</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HscB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB28">
        <property type="interactions" value="339"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB28"/>
    <dbReference type="InParanoid" id="A0A1E5RB28"/>
    <dbReference type="OrthoDB" id="448954at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051259">
        <property type="term" value="P:protein complex oligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.20">
        <property type="entry name" value="HscB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004640">
        <property type="entry name" value="HscB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036386">
        <property type="entry name" value="HscB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009073">
        <property type="entry name" value="HscB_oligo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00714">
        <property type="entry name" value="hscB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14021">
        <property type="entry name" value="IRON-SULFUR CLUSTER CO-CHAPERONE PROTEIN HSCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14021:SF15">
        <property type="entry name" value="IRON-SULFUR CLUSTER CO-CHAPERONE PROTEIN HSCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07743">
        <property type="entry name" value="HSCB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47144">
        <property type="entry name" value="HSC20 (HSCB), C-terminal oligomerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="4">
        <location>
            <begin position="29"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="148"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ84081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="212" mass="25019" checksum="39501609E7B68BA7" modified="2017-01-18" version="1">MLKQLGFSTMKMTNLLRHSYRIQNKRHVSFYELFPKTLAKSDSDKWNLNLNQLRKEYRVLQSRSHPDLVNHNADTSAKQDQLDSSLLNQAYNTLKNPLLRAQYLIQLETGKNLNQDSEVQELKKTAISPEILMDVMLVHEQLEDATSETEVKEMREENKQKMEKLEEKLGECFNKTHDYXEAIKLALTLRYWTNLDQAFKEWDPNRPVTLNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB79</accession>
    <name>A0A1E5RB79_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RB79"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="405" mass="47273" checksum="088EA4B079861B8D" modified="2017-01-18" version="1">MSELIDNIVFXYNENDYLCDILNGNKTTENNSEDFGVFDVEIDRPYTPINKIIPENEIFEENLNEMNNEIYDDKXDINNTKRDDGTEXYDLKYTDIRLTTENYYKYYSLTKDKPILPNIQVNGLENSSLGQQXLSSPFCYNKVSLFNNEVDSEFNDNDFSLDYTLDSNGEYNNNNNNNDNNNNNNNNNNNDXWDDTLLNDYTVWETNTPMSLNMEHKISTNEVNESYMDMKYXCDINNNXKNKNDKNFTKTNQKCYDNSALKIIKSNTESGLNSXSHTRRPSDNYHTLKNACNFIHTNNNTQMKRFSNVKEISQGLKIFKKRFKSNANSMLTMGSSKPTVHQLNYYNNDNKLHFNNELTNKLENYIVAMADDKTCCANDSFLRGKTEQNEERIKEIGVRLSKTYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB81</accession>
    <name>A0A1E5RB81_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein SPT3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2655</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SPT3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RB81"/>
    <dbReference type="FunCoup" id="A0A1E5RB81">
        <property type="interactions" value="1142"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB81"/>
    <dbReference type="InParanoid" id="A0A1E5RB81"/>
    <dbReference type="OrthoDB" id="66982at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22926">
        <property type="entry name" value="HFD_SPT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000023">
        <property type="entry name" value="transcription initiation protein SPT3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003195">
        <property type="entry name" value="TFIID_TAF13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID/SUPT3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380:SF16">
        <property type="entry name" value="TRANSCRIPTION INITIATION PROTEIN SPT3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02269">
        <property type="entry name" value="TFIID-18kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="104"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="251"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="104"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="251"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ84158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="333" mass="37469" checksum="78F7D8292FF993A8" modified="2017-01-18" version="1">MEIQQMMFVSGEVNEPSVATTSLIEDIVRGQVVEILMRSTTTAHARGSKNIMPEDVIFLMRHDKAKVNRLRTYLSWKDLRKNAKDQDNNAALGGADAVDAAEGGAGGSGVGAGGAGGAGEGKDSKDPANMKLKKSTIKLPWELQFMFSEQPLQNNEDDDDDDEREAHLETMKRLKMADDRTKDMTREEYVHWSDCRQASFTFRKGKRFKDWSGITSLIDGKPHDDVIDILGFLTFEIVCAITETALKVKAKDERISKEKTDQSQKHDAQDDDSVNGTIRKRKRRLFDGPENPVNPINTSNVQEAWRILQIQDHRHRALGNFKGQRARSRPMIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBA5</accession>
    <name>A0A1E5RBA5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cAMP-dependent protein kinase regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2771</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cAMP-dependent kinase regulatory chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBA5">
        <property type="interactions" value="453"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBA5"/>
    <dbReference type="InParanoid" id="A0A1E5RBA5"/>
    <dbReference type="OrthoDB" id="417078at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000039">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000118">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00103">
        <property type="entry name" value="CAMPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0114">cAMP</keyword>
    <keyword evidence="4" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="9">
        <location>
            <begin position="318"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="9">
        <location>
            <begin position="436"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="122"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="210"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="123"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="227"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ84179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="551" mass="61207" checksum="372FD4E3639F3146" modified="2017-01-18" version="1">MKHRLNHESEQDNKKTRLAIXCTXDIREXRDNTEIQGNKEVKDRKPRNRENIEPRENIEPRENIEIIKTNTMNQYQSPELNXFIASLNSEKPDNFVTYGLKYFSERAVELLSTSTTISNNNNPAQNSGMFSNSYSIGGNNNNNNNGKKSASVVSLNNGSSHRGSIFKSPFVDTDPHTDNTSDVSIDKANFSSGGKANVSSNLFKGDFNVGNHISQSKEPELDPMAPAPTSTTATTTTTTNTTSNNSSASTANDQHTSHGKILPFNFNAQRRTSVSGETIQPEKDDIEMRALYERIKKLNNKTSEQLEKLSASVGENFLFKKLDTDSKKLVITALVXKHISKGETIIKQGDEGDFFYIVENGQVEFYVNGTKVSTSGAGSSFGELALMYNSPRAATVVASTDCGLWCLDRLTFRKILLGRSFKKRILYDEFLKKVPILSKLTTYDRAKLADALNTKFYSKGDVIIKEGDVGENFYFIEYGSALVLKKGHNDPLTTLQKGDYFGEVALLNDLPRQATVMANENTKVVTLGKSGFQRLLGPVVEVLKLHDPTRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBG7</accession>
    <name>A0A1E5RBG7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2899</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ubiquitin conjugation factor E4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBG7">
        <property type="interactions" value="1275"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBG7"/>
    <dbReference type="InParanoid" id="A0A1E5RBG7"/>
    <dbReference type="OrthoDB" id="20295at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034450">
        <property type="term" value="F:ubiquitin-ubiquitin ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16657">
        <property type="entry name" value="RING-Ubox_UBE4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000055">
        <property type="entry name" value="Ubiquitin conjugation factor e4 a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019474">
        <property type="entry name" value="Ub_conjug_fac_E4_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045132">
        <property type="entry name" value="UBE4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13931:SF2">
        <property type="entry name" value="UBIQUITIN CONJUGATION FACTOR E4 B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13931">
        <property type="entry name" value="UBIQUITINATION FACTOR E4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10408">
        <property type="entry name" value="Ufd2P_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="11">
        <location>
            <begin position="991"/>
            <end position="1065"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ84236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1066" mass="123229" checksum="FE7DB4AC8E1C6B4D" modified="2017-01-18" version="1">MSNNNNTSTASMIEDIFKVYIDPSRKTDSNWTLIESEDFHGVESLSMDHLDSIILHQLTEVSGLPFYENNPFXYLTSCYNDCNVYKKTLKSNKNIDDDDDDDHHHNNNKNVFEDNVAACQDAISNYGLILLQLDEFSSYGSQFEMVKYIIENLNDCNEFLVAIIHKAIEQEELLTVCNTLFNQYMVFIQDDAVSSSSSSSSSFSSSSNSINDIRLSMNDPLLYNQVLNYFEFLCSIKPIGAIITQLDSFDPLKISPLTDSKTRNDPNLKINMVEQKSLLGTILRLSPLQKNVTLGSYKSLAVYEEEDLRNNSSIDREITQIHETLQLEHNVIINKLFFILNKLFRSGNNTRSQLLNWLGAIVNKNHLRKGDHHDPRKLSSDAFMCNLSILMIKFSLPFLDIDYTKLDKIDMDYFKHPFKISIDIEDETRMNSDLKEYKEQYSVNDINDPYETNFISDCFYLSCTFLHYGIGGAILNDSIYERRSKQLKEELKKIDYAIKQKTRTLGGNAGEFVVKMFQETKNKIQNQMLEMKSYRLSFQGFFIHKFLQMDVFSFICGVSTFLIRVVDPRKEYPKTQIXLPLLPDQLGLENVDNFEYLRSNAPKPFKYFPEFMVDGLIDYCQYINRYYMSPMIKNPRLGSFLDLCTVLLRCPELISNPHLKSKIIVTLSIGCTPQRRKAFGSDEDDDMDMDLDDEGSGDFLNNGSVVPGFMMEVIETDEICLENLIFSLLDFYVIVEKTGSSSQFYDKFNSRYYISVILEQIYKIPYYKKSLFNLVDTNLDFFTRFVARMLNDLTFLLDEAFTSLIEVHDCYVEIQKRKRGEVSSSNEEESFEKLKARFLNAGSKAKSSCGLANKSMNLFKIFTRDVAPAFTIPEIVDRLASMLNFNLKILVGPKCNNLIVKNPEKYSFEPKKLLKNLCSIYINLSNEENFILAVSKDERSFDKKLFDQAINNLYNRLHIVDAEFAESLQNFVQNVVEQKMSNEQEELDMNDCPDEFLDPLMYTVMNDPVRLPQSNVIIDRSTIKQHLLSDSTDPFNRSPLKLEEVIPETDLKLQIEQYKKNKXAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBI9</accession>
    <name>A0A1E5RBI9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 15">Saccharopine dehydrogenase [NAD(+), L-lysine-forming]</fullName>
            <shortName evidence="15">SDH</shortName>
            <ecNumber evidence="5 15">1.5.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 15">Lysine--2-oxoglutarate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">AWRI3579_g2466</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>L-saccharopine + NAD(+) + H2O = L-lysine + 2-oxoglutarate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12440"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:57951"/>
            <dbReference type="EC" id="1.5.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the AlaDH/PNT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.7" evidence="5 15"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBI9"/>
    <dbReference type="FunCoup" id="A0A1E5RBI9">
        <property type="interactions" value="208"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBI9"/>
    <dbReference type="InParanoid" id="A0A1E5RBI9"/>
    <dbReference type="OrthoDB" id="265306at2759"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00034"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004754">
        <property type="term" value="F:saccharopine dehydrogenase (NAD+, L-lysine-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd12188">
        <property type="entry name" value="SDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000217">
        <property type="entry name" value="Saccharopine dehydrogenase [NAD(+), L-lysine-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000423">
        <property type="entry name" value="Saccharopine dehydrogenase [NAD(+), L-lysine-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051168">
        <property type="entry name" value="AASS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007886">
        <property type="entry name" value="AlaDH/PNT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007698">
        <property type="entry name" value="AlaDH/PNT_NAD(H)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027281">
        <property type="entry name" value="Lys1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133:SF23">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE [NAD(+), L-LYSINE-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05222">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018250">
        <property type="entry name" value="Saccharopine_DH_Lys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01002">
        <property type="entry name" value="AlaDh_PNT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01003">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11 15" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="9 15 17" id="KW-0520">NAD</keyword>
    <keyword evidence="8 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alanine dehydrogenase/pyridine nucleotide transhydrogenase N-terminal" evidence="20">
        <location>
            <begin position="6"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Alanine dehydrogenase/pyridine nucleotide transhydrogenase NAD(H)-binding" evidence="19">
        <location>
            <begin position="185"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="16">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="203"/>
            <end position="204"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="318"/>
            <end position="321"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="205"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR018250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR018250-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR018250-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR018250-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM01002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM01003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OEJ84264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="373" mass="41440" checksum="6E48FCCAF63D427B" modified="2017-01-18" version="1">MSVILHLRGETKPKEARAALTPTTVQKLISQGFTIYVEESEQSTFNINEYKKAGAKIVPFGSWKTAPLDRIIIGLKEMPETDTFPLVHEHIQFAHCYKNQAGWQGVLQRFIDGRGTLYDLEFLVNDQGRRVAAFGFYAGFAGAAVGLKDWAFKQYSSDNKDLPGLSPYPNEKALIKDVSKDYKKALKYTKGRTPKVLIIGAKGRCGSGAVDCLTKIGVPQENILKWDIDETKKGGPFKEIADADIFINCIYLSKPIAPFINYDLLNQDDRKLRTVVDVSADTTNPHNPVPIYSIATVFDEPTVLVPTTKGPKLSVVSIDHLPSLLPREASEFFAADLLPSLLSLPQRNTAPVWTRAKALFEKHCARVTRSSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBL9</accession>
    <name>A0A1E5RBL9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="9">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g3117</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. UvrD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="9"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBL9">
        <property type="interactions" value="262"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBL9"/>
    <dbReference type="InParanoid" id="A0A1E5RBL9"/>
    <dbReference type="OrthoDB" id="1470711at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17932">
        <property type="entry name" value="DEXQc_UvrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.486.10">
        <property type="entry name" value="PCRA, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013986">
        <property type="entry name" value="DExx_box_DNA_helicase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070:SF2">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE SRS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51217">
        <property type="entry name" value="UVRD_HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 11 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="13">
        <location>
            <begin position="7"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="14">
        <location>
            <begin position="308"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="524"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="694"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="867"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1015"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="528"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="694"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="867"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1021"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1039"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="28"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="15">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="15">
        <location>
            <position position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ84286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1078" mass="123187" checksum="9F6A7A2722F7F252" modified="2017-01-18" version="1">MQEFSNQLNDNQYRAVVFDPKKVLQVNAPPGTGKTKVIISRIVYLLTVAKLPPESIICTTFGVKAKEEIVDRLSKALENTDINYTQVNVGTFHGICYGILRKHGYRIGCTDLEVISDDDQEKLMKKMIENVPPHILDAASSFLRKKNLMMMNKDGAYVLSVKLIKSNISKLKSQGQTVKDYSDAHDVDQALLYFYENYQKAMKDQKKVDFDDLLLQAYTLLKEHNCLKQVQHVLVDEFQDTSVLQMQLMFLFARGKSVNCNGITVVGDPDQCIYAFRDAMPHNFKDMIRISPVPVTTITLNQNYRSTQRIVDSCQNLMKQQQKGRDNTTKLFAQYDTKFGPVFHSYHNFKEEAAGLVSEIMYLKSLPGLFSYNDFSILVRQRRQIPALERALIEARLPYXIIKGQSFWERREVKSIMNNIKLVLSDYEPFALEDALLYPARGLGKAGFEKIKNKLSDEANSFNQLKKYYNLLPTKGRKVATKLIETIETARAMFENGTSKSNMEEIFDYIYENSGLKHELTVNKKDTNPSQQKASVQKSGDFEEAPDLSSPKHMNVMQVKEQLMEFSNDYSQMNVDDEEEDNDFIFSLSRTSSALXSKVEDNELDDLDTAEVVEKPNECSKTPEIIYDKSFVREFVSSVRLYGDKESEDEESQFKEGRVTVCTTHKAKGCEWPVVFVPGCTEGTNPSLFANDVEDEDSEDEXEAANNTNKPGKYKKTMDELLNEERRVFYVALSRAKVQLYLSSVETSSERNHETEYKLVRSRFITPGFLKTVEMAQQLFXNVENIKSFYXTMGVKFQKNISNFSLQQLIDDYDSYLNDERLHFHWMGSQVYVKNGINLSFNHISASPKKYSSGFLSAAEQLGSSLSLNATSHNRQTLAPRPSGRSRQPKELPNIRXNLDXTXSRFVNKSPTRKTYAPTKNLNLSPVRRKVYAPSKAPVSNNSPNVSPEKKKLKKDFKIAXSNERKNQKKPXLDLPTGRTPTIDLTAAEILHDPEEETVDHRPFLTSANSLLERSRKAKLKKEGDDVKKEHDENLSQHPLKKARKNNKKNTQQPPEGKDIFSLLKKGKKIDRAYIEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDG3</accession>
    <name>A0A1E5RDG3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable electron transfer flavoprotein subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2337</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="7 8">Binds 1 FAD per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ETF alpha-subunit/FixB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDG3">
        <property type="interactions" value="503"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDG3"/>
    <dbReference type="InParanoid" id="A0A1E5RDG3"/>
    <dbReference type="OrthoDB" id="1715808at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01715">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000001">
        <property type="entry name" value="Electron transfer flavoprotein, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001308">
        <property type="entry name" value="ETF_a/FixB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033947">
        <property type="entry name" value="ETF_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014731">
        <property type="entry name" value="ETF_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153:SF1">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00766">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000089">
        <property type="entry name" value="Electra_flavoP_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 7 8" id="KW-0274">FAD</keyword>
    <keyword evidence="4 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="9">
        <location>
            <begin position="34"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="289"/>
            <end position="290"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="303"/>
            <end position="307"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="320"/>
            <end position="327"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000089-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ84937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="373" mass="40161" checksum="2A6C841A6D912D9D" modified="2017-01-18" version="1">MIRNSTRSSVNGLRTLLSQHSSTLTKSTRLHQSTLAFIETNTDGGILKHSQSALEASKKLNASEGVVAVLVGSSSDKAVPNLTKLAQTYNIQKILYSDNSALDHYSPELVTPLLQQILSSNGNSEISHILFSASAVGKSILPRLSATLNVQCINDIVDIKDKKTYIRPIYAGNLLSTIENNQKTQLISIRSSAFPXQESLDQQENSGAAPQVVKVEYTTPSAQEVHAAEYVXSKLLESERPDLGSAQVIVSGGRGLKNKENFDKLMIPLADAFAKLPKFKNVPAAIGATRAAVDSGFCDNSLQIGQTGKVVAPELYVACGVSGAIQHLAGMKNSKVIVNINKEEDQPLQDISDYTLIGDVNEILPELTEKLVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDG6</accession>
    <name>A0A1E5RDG6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 15">mRNA cap guanine-N(7) methyltransferase</fullName>
            <ecNumber evidence="3 15">2.1.1.56</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 15">mRNA (guanine-N(7))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12 15">mRNA cap methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AWRI3579_g2024</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Responsible for methylating the 5'-cap structure of mRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:67008"/>
            <dbReference type="Rhea" id="RHEA-COMP:17166"/>
            <dbReference type="Rhea" id="RHEA-COMP:17167"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:167617"/>
            <dbReference type="EC" id="2.1.1.56"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0 methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.56" evidence="3 15"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDG6">
        <property type="interactions" value="1037"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDG6"/>
    <dbReference type="InParanoid" id="A0A1E5RDG6"/>
    <dbReference type="OrthoDB" id="10248867at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004482">
        <property type="term" value="F:mRNA 5'-cap (guanine-N7-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000280">
        <property type="entry name" value="mRNA cap guanine-N7 methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004971">
        <property type="entry name" value="mRNA_G-N7_MeTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016899">
        <property type="entry name" value="mRNA_G-N7_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039753">
        <property type="entry name" value="RG7MT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12189:SF2">
        <property type="entry name" value="MRNA CAP GUANINE-N7 METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12189">
        <property type="entry name" value="MRNA GUANINE-7- METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03291">
        <property type="entry name" value="mRNA_G-N7_MeTrfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028762">
        <property type="entry name" value="ABD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51562">
        <property type="entry name" value="RNA_CAP0_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15 20" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9 15 17" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="5 15" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 15 20" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MRNA cap 0 methyltransferase" evidence="19">
        <location>
            <begin position="128"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="27"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="34"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="137"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>mRNA</name>
            <dbReference type="ChEBI" id="CHEBI:33699"/>
        </ligand>
        <ligandPart>
            <name>mRNA cap</name>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="site" description="mRNA cap binding" evidence="17">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <feature type="site" description="mRNA cap binding" evidence="17">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="site" description="mRNA cap binding" evidence="17">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <feature type="site" description="mRNA cap binding" evidence="17">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <feature type="site" description="mRNA cap binding" evidence="17">
        <location>
            <position position="335"/>
        </location>
    </feature>
    <feature type="site" description="mRNA cap binding" evidence="17">
        <location>
            <position position="404"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="20">
        <location>
            <position position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR028762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR028762-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR028762-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OEJ84950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="413" mass="47982" checksum="7679E328070BFDD6" modified="2017-01-18" version="1">MNSAPPKAPWMSDAEYQRLYGHLLNTKTEEKLELTSSTSNPVEETNTTTENKDKEKYSVSKKRRGLDSSSYRDEEYLERQKREKLLAEQIKKQEINLTAGGKVNVDHMVIQHYNERTIMTKNENRDLSPIIKLKNFNNAIKYMLIHKFTKPGNVVLELACGKGGDIRKYASCNISQLIGIDISEESIREASRRYQKMRNSISYEVVFITGDCFGESLGPTVEPFPTCRFPCDAVSVQFALHYAFETEAKARTMMMNVTKSLKIGGYFFGTIPDSEFIRYKLNKFPKDREEIKWGNSVYRIKFADNEYLKNDYEFPSPFGQMYTFFLKDAIDNIPEYVIPFETLRSLADEFGLELRLKKGFNEFFAQEFPAWYNQFSPRMKXGLKRKDGKYGIEGDEKEAAAYFYTTFAFQKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDL1</accession>
    <name>A0A1E5RDL1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">E3 ubiquitin-protein ligase PEP5</fullName>
            <ecNumber evidence="9">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g2081</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex. Component of the class C core vacuole/endosome tethering (CORVET) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Vacuole membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the VPS11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="9"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDL1">
        <property type="interactions" value="617"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDL1"/>
    <dbReference type="InParanoid" id="A0A1E5RDL1"/>
    <dbReference type="OrthoDB" id="26184at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033263">
        <property type="term" value="C:CORVET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048284">
        <property type="term" value="P:organelle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16688">
        <property type="entry name" value="RING-H2_Vps11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057308">
        <property type="entry name" value="CHCR_PEP5_VPS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057307">
        <property type="entry name" value="PEP5_VPS11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016528">
        <property type="entry name" value="VPS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056939">
        <property type="entry name" value="Znf_RING_Vps8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323:SF24">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 11 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23341">
        <property type="entry name" value="PEP5_VPS11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23356">
        <property type="entry name" value="TPR_PEP5_VPS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23412">
        <property type="entry name" value="zf_RING_Vps8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007860">
        <property type="entry name" value="VPS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0926">Vacuole</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="CHCR" evidence="11">
        <location>
            <begin position="429"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="929"/>
            <end position="974"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR007860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ84992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1052" mass="119974" checksum="E10444696B922A27" modified="2017-01-18" version="1">MSLSSWRQFQFFDVQQLNDPLTSDKRPLFSEHSLSAIAPLTLQTSPEEIHFKKYTNSDLLVVSYNSTQIKIISLVDSEILVDYNAYNETDPYRITHVRSLEKISSVFLTVAECAGKPALIKVWKFGSHLTNPDSFLSLVEAKDKEHGNMFPISAMDTSATLNCIAVGYANGLVILIRGDIMRDRGYKQRIIYSDAKNEPITSLLFDSTVTHLFVGTSSKIFTLSTNGRNNRELDLLLNDHGGVLLNCATKDEKKRFACLXEDSIDWYTSKGEKTSLALDIQNKARFFFIDPMHYLIVXSVDTTSYTSKNFAQVSNFQIXIVDIATRVVSMSTTVTSSIIDVFNFNYKRESCVLALLGDGKLLRITEKNIKDQLSIVIQRELYPVALNLAEKHYSETNENQVPEIKKLYGDYLYKKDEKTKAMDQYIEXLDVIEETSEIISKYSIAQNGSSDSVNDLSRFLWALLRNGCAHTDHITLLIITLVKLKDQKNINFFIDHYSRAGKLVDTADDYNYTFEEDEEFFYSDVELFDLEKVLKLLVEAGFKEEAYKLCRKFSKDPSSIVEVLLNVLNDPRKALNYAKSLPVDDTLRVLLTYSKKLLDYLPNDVNALLIAVFTGVYKPEVYVTSRKAEEKQQKTSDXENFNSIVFHTYTSFFQYSSKINEDQKQASLLSEPTYHPPRPSLIFTSFIHKPYEFVVFLEACLEAYNKFKGFDNDKQDVLTNLYEVYLSLGEDDEVVERRESWKQKAXQIYKKVEETETFEPDNSLMLLISDLHKFSVEKDDLDRNEITSLFRQMTLNSSTETVMHFLKKHGEQEPELYAIALKYSISSKTILENVGGEDIXKKDILTPMIHAGRFDMLHLIELLSSSKVATFGLIKDMLINHIQEESHSLDTNKKLLHSYKDELDKVNTELKVLLTENEPVEINLDREVCSICQTALELPVIYFKCGHSYHQKCLSEEDYDTLKNGEAVYKCPKCVVELETAKKLASAQADFAQKQNQEFELALPVSLHDASTNINGSSTPGTKSSYEINDKFGAVFELISKGALESSRVTID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDM2</accession>
    <name>A0A1E5RDM2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SAGA-associated factor 73</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1899</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDM2">
        <property type="interactions" value="423"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDM2"/>
    <dbReference type="InParanoid" id="A0A1E5RDM2"/>
    <dbReference type="OrthoDB" id="21678at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041251">
        <property type="entry name" value="Znf_C2H2_13"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18508">
        <property type="entry name" value="zf_C2H2_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAGA-associated factor 73 zinc finger" evidence="2">
        <location>
            <begin position="69"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="416"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="416"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="441"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="476"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="619" mass="69731" checksum="F021C93099C66E6B" modified="2017-01-18" version="1">MSGEISKIKNEIAEKYALSTGKESQISKGWQNLVTNVNLQRKKAQSDSNSIENKNPEIYQQNWRDLFSSNVEILPNPIQNRINLRVCNNCNKPLTPTAIQKHVAFCNNPKQYLETKILKASNNINNKKDIKQEQPISPVKDNTAKEKSVKPNGKRKLEDDVALDSPSTPSKQGSPGPKRRRNNNNTKSESQAQTPQPEAMDSVESSAPSSAPTKQQAKISKQMEKKQKLAKKQEERALQKRLKQEERVKQQQLKKHQRLKTQQEKALKLQLKQQQQQLLLEKQQPKKLTAEEETHNVLSGISRSYPLPLENCVLSSTRLKTKNFRLRQMFSKCFNTKQGFTKPGFGALEGRLGYVDVERPNEYAFKIDFSHSKYTPAQQQQMLMQQQQQQLLNQQQILHQQQQQQQQQQMSALSPQQVLAQQQKKMAQQGPAANAMPQNTNPQQILMLQQQQQKLQHQQNQQQQPNQQPPDPLIFQKMQQQRMKQQQAQMQALQKQQSSQNTNSGVNTNLNLNLNGPVQAGLVPTPQQQRAQEAAMHLAASTQMRQXQNTHLQGASLNGNFATNNNGNNVPAGTPKSTPTMNNGITTPVAAPNSATGGVQQGLGSAVNIGITSPFQPNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDP0</accession>
    <name>A0A1E5RDP0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 20">Aurora kinase</fullName>
            <ecNumber evidence="2 20">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">AWRI3579_g1894</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
            <location evidence="1">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2 20"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDP0">
        <property type="interactions" value="1078"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDP0"/>
    <dbReference type="InParanoid" id="A0A1E5RDP0"/>
    <dbReference type="OrthoDB" id="377346at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032133">
        <property type="term" value="C:chromosome passenger complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051233">
        <property type="term" value="C:spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045143">
        <property type="term" value="P:homologous chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044779">
        <property type="term" value="P:meiotic spindle checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
        <property type="term" value="P:regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090266">
        <property type="term" value="P:regulation of mitotic cell cycle spindle assembly checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902115">
        <property type="term" value="P:regulation of organelle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072479">
        <property type="term" value="P:response to mitotic cell cycle spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000819">
        <property type="term" value="P:sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14007">
        <property type="entry name" value="STKc_Aurora"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000235">
        <property type="entry name" value="Serine/threonine-protein kinase ark1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="8 20 23" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="7 16 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 20" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="123"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="47"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16 18">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="250"/>
            <end position="251"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="17">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU367134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="OEJ85009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="381" mass="43645" checksum="DE4735CF09778BCB" modified="2017-01-18" version="1">MSSKQFSSRNRVSAASRTDTDKDKKVYMRTYGKTPSTPLGIAKNLNVPAQTMSXHSNLSPTKQLTSSRSPNKRNFSMKPTNPSSGIKPATRSLSSKETRTSSPMKNSASSDYSIQRNMSIKDFQIGKKLGKGKFGRVYCAKHVTSGFIVALKVMDKKELLHYNITKQLRREVEIQSRLKHPNITQLYGYFHDDKRVYLIMEYCQGGELYKYLKKRGTLNDITASNFVHQMLSSLSYMHGKKIMHRDIKPENILLGSTGQIKFTDFGWSTEYVKNSKRKTLCGTVDYLSPELIKSREYDQQVDLWALGILTYELLCGKPPFEEATKDLTYKRILERDLKFPTHISPDAVDLISRLLALNPKERISLSDALKHPWITKHQPYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDP4</accession>
    <name>A0A1E5RDP4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Putative metabolite transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2043</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDP4">
        <property type="interactions" value="794"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDP4"/>
    <dbReference type="InParanoid" id="A0A1E5RDP4"/>
    <dbReference type="OrthoDB" id="4540492at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015149">
        <property type="term" value="F:hexose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF8">
        <property type="entry name" value="FACILITATED GLUCOSE TRANSPORTER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="220"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="345"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="385"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="409"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="435"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="472"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="497"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="52"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="547" mass="59965" checksum="AD7549B57D7FA32D" modified="2017-01-18" version="1">MSENAPLLGVPPVPAASDMPEVTNNTNNTTNRSQRSQSFWKKNSLTWRLALIILITCMGSMQFGYHMAELNAPQRYLTCDYLHGDVSPKGSSLSSSSSVSSSVFNNLKQTQRLKTCVEMSDQQYGVITAIFSIGGLVGSFYAGRFADYMGRKNLSIVNAMMSLIGSLVLTLSDSYWQFIVGRLIVGMAAGSSIVVVPLMITEIAPVSMKGVFGSMNQGSINIGILVVQFFSLFWARPKKWRYLFVAGIVIACIQLILLVFLFEESPKWLYNKGELRKAEKVLXGLRPGSRTEVKREIEEWGHQSRNSQASGSVGAGMGATNSTPYNRDVTFWEYLTGPRYFKTRLLVTVILVGQQFTGINSIILYGVSVVGNLAKNSRTPIKVNFGISILNVVVTIGASPLIDHFGRKPLLISSSVLMGIAAYMVXIGLHFEKLVFLILMIFGYIGFFALGVGPIPFLIIPELSPSNALGAAQSYGTVCNWIGTFIVAYGFPVLSDMIGITSVFLMFATFAILFAIYLFFTLPETRGKSNYNEVWGIDSSSSSPYQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDQ3</accession>
    <name>A0A1E5RDQ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g2223</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">High-conductance magnesium-selective channel that mediates the influx of magnesium into the mitochondrial matrix. Essential for the splicing of mRNA group II introns in mitochondria by affecting mitochondrial magnesium concentrations, which are critical for group II intron splicing. It also suppresses a variety of mitochondrial intron mutations and its absence may disturb the assembly of mitochondrial membrane complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homopentamer. Forms homooligomers. Interacts with MFM1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Mitochondrion inner membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDQ3"/>
    <dbReference type="FunCoup" id="A0A1E5RDQ3">
        <property type="interactions" value="358"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RDQ3"/>
    <dbReference type="OrthoDB" id="10251508at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045016">
        <property type="term" value="P:mitochondrial magnesium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12823">
        <property type="entry name" value="Mrs2_Mfm1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039204">
        <property type="entry name" value="MRS2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890:SF27">
        <property type="entry name" value="MAGNESIUM TRANSPORTER MRS2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890">
        <property type="entry name" value="RNA SPLICING PROTEIN MRS2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22099">
        <property type="entry name" value="MRS2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 14" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="377"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="407"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU366042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ85038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="512" mass="58502" checksum="2054CD4D1EFC466E" modified="2017-01-18" version="1">MQKFSAATHHNLIRSFTNGSRFICARRLRSMSFDTALRKYTIAHGVLRNFGAFRTRTYNQVTSAITQLRGLSTTSPLLQNERSLDNLRHQLHQYTASLNPLKPVVPNDLFVSCTIMKKNGTVVGISQKFPKWQFLKDHDLYPRDLRKIDTSTIDNIPSIVVKPNKCILVNLLHIKALIKANEVMIFDTVNPVAAHGLGELMYDLEVRFQHSTAQLTLPFEFQVLEIILINVLNSLEVQLNNLEKQCGGILKDLENEIDRNKLKDLLIRSKNLSAFYKKTLLIRDVLDEILDNDEDLQGLCLTNNTNKDSEALPNQNHGNLLESAEDVEMLLETYYYQCDEFVQKSESLIQNIKSTEEIVNIILDSNRNSLMLFELKITIYTLGFTVATLLPAFYGMNLKNFIEESDLGFAGVILVSSLAGIIITMTNFKALRSVTRLTLMNNHSGKNTKTHIDNAEMKTQKFVPTVSSNLKNWKRKLHYLMKGENSKIAQVDRLTSKREAIRQWLIQHKTDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDU9</accession>
    <name>A0A1E5RDU9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chromatin target of PRMT1 protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2454</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDU9"/>
    <dbReference type="InParanoid" id="A0A1E5RDU9"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="231" mass="26020" checksum="037B25AC10D70924" modified="2017-01-18" version="1">MSEEITQSTAVSTGDNVPVSIETQEQHPQQTNDLDMDVEENAADSSATTEGAHETVNDHAVSSANENTDVQPKDNSNRKITYYRSRSLRTGLGKRLGFNSVQEMQQLREPEEPYGDYDRSNHGPYSGQYERRAPRFHGTASSYSPGPSPSGGRRLIDILDNGRYKSKNYRRSKGTSNGSGFDHDHGHDHHHHQNVNKGRHPRGKKQRSKVETPKRSIEDLDKELEEYMKGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE12</accession>
    <name>A0A1E5RE12_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Non-histone chromosomal protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2153</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NHP6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RE12"/>
    <dbReference type="FunCoup" id="A0A1E5RE12">
        <property type="interactions" value="448"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE12"/>
    <dbReference type="InParanoid" id="A0A1E5RE12"/>
    <dbReference type="OrthoDB" id="1919336at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008301">
        <property type="term" value="F:DNA binding, bending"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001195">
        <property type="term" value="P:maintenance of transcriptional fidelity during transcription elongation by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070898">
        <property type="term" value="P:RNA polymerase III preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01390">
        <property type="entry name" value="HMG-box_NHP6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000016">
        <property type="entry name" value="FACT complex subunit SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF22">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION FACTOR A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00886">
        <property type="entry name" value="HIGHMOBLTY12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="8">
        <location>
            <begin position="22"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="6">
        <location>
            <begin position="22"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="94" mass="10622" checksum="B0513B7253613F8B" modified="2017-01-18" version="1">MAAPRETKRKAGTRKKKDPNAPKRALSAYMFFANENRDIVRAENPGISFGQVGKMLGEKWKQLSEDEKAPYDAKAGADKKRYESEKQLYLATKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE25</accession>
    <name>A0A1E5RE25_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative tubulin--tyrosine ligase PBY1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE25">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE25"/>
    <dbReference type="InParanoid" id="A0A1E5RE25"/>
    <dbReference type="OrthoDB" id="202825at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1210.10">
        <property type="entry name" value="Survival protein SurE-like phosphatase/nucleotidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002828">
        <property type="entry name" value="SurE-like_Pase/nucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036523">
        <property type="entry name" value="SurE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027746">
        <property type="entry name" value="TTL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00087">
        <property type="entry name" value="surE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47551">
        <property type="entry name" value="TUBULIN--TYROSINE LIGASE PBY1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47551:SF1">
        <property type="entry name" value="TUBULIN--TYROSINE LIGASE PBY1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01975">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64167">
        <property type="entry name" value="SurE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Survival protein SurE-like phosphatase/nucleotidase" evidence="1">
        <location>
            <begin position="11"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="794" mass="90479" checksum="90B1C7E5D80E4438" modified="2017-01-18" version="1">MTQDLSSRLNILLTNDDGPLDEFVSQYVRPFVQYITQHKKNWNLVICVPDSQKSWIGKAHFAGKNLXCTFLYSNSKSSNNNDFIGPFSKPLLYNTNTKFKPSTNGITFPYTNPTTNQTFKTYTDLKNYYDLEWCCINGTPASCCDIGLHHITXIPKFDLVISGPNVGRNCSRPYITSSGTVGAAFESCITGNTKAIALSWAYFDGKKNFDESTYATASKISCDIIESLYGQWGIGGNHSEVELYSVNVPIHQELHENNTKAIFTPLLDNKWCSIYDTVESDESVVFAWNPDFQHNRDQMLQSLESGEINDGAVIEDKQISITPLFASFNQAIDAKSIGKTLDYQAASFLSNTFLKTSSDDXAKDSTXALLCMSIDSKEYIYQPIINAVQKYTPHVSIVTAIPTEFSQNSETDNNEKNLKIVQYADYEDIDHEFLNSIASQKTFATNTYTYRKSLIRKHYLNNTIKMYCSKNPESILNSAFMDSYHLELDYAEFLDDSLDDNWELRQDLEEESEDKWWIVKPSMSDKGQGIRVFKTINDLQAIFDSFDEGVETDDEYEEEQADGNDPTTTKDDNKIIISQLRHFIVQRYLTNPLLLPQMDSKKFHIRCYVLCNGDLDVHVYDRMLALFAPDTYEMPSTDQDLNRSTQKLQCHLTNTCLQENKENSVREFDTLNDLTVEQKQSVKTQIHEIVKDLFMAATNDKMNFQAIPNAFEIFGLDFLVDNHYNVKLLECNSYPDFKQTGDDLKDLIDELFDGVAKQAICPRLTFSNAFPFSSLTEKAPENFTQVLSSTGKNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE28</accession>
    <name>A0A1E5RE28_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RE28"/>
    <dbReference type="InParanoid" id="A0A1E5RE28"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="76" mass="8584" checksum="0A561C5966BB277C" modified="2017-01-18" version="1">MSALQQWIGIAKRQVRNVRRTAETIANEILQPVEQQAQPQLQRVPIPVNENNGQSRLPFPVNRGNSNNFNGSDNFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE53</accession>
    <name>A0A1E5RE53_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Lysophospholipase</fullName>
            <ecNumber evidence="2 9">3.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1833</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the lysophospholipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="2 9"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RE53"/>
    <dbReference type="FunCoup" id="A0A1E5RE53">
        <property type="interactions" value="106"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE53"/>
    <dbReference type="InParanoid" id="A0A1E5RE53"/>
    <dbReference type="OrthoDB" id="4084751at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07203">
        <property type="entry name" value="cPLA2_Fungal_PLB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000010">
        <property type="entry name" value="Lysophospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF33">
        <property type="entry name" value="LYSOPHOSPHOLIPASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8 9" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6 8 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009028103" description="Lysophospholipase" evidence="9">
        <location>
            <begin position="23"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="domain" description="PLA2c" evidence="11">
        <location>
            <begin position="35"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="619"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ85181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="673" mass="72857" checksum="8A89CEB52C1C6D6F" modified="2017-01-18" version="1">MKGSIAVSSAASLLMLSNMAYAWSPSDSYVPANVTCPSNFNLIREASGISDKESDWLAKRDNLTTPILKNWLSDKMTNVTDHDSLLSSVFNSNNGSSSFHTPRVAIACSGGGYRAMLSGAGMLSALDNRTDGANEHGLGGILQSATYLAGLSGGNWLTGTLAYNNWTSVQDIVNNMTQSDSIWDISDSIINPGGWNIFSTVDRWDDISDDVQDKKDAGFNVSLTDVWGRALSYNFFPTLKDAGAGYTFSTLQKSDVFENAEMPFIISVADGRYPGTYILDTNSTLFEFNPFEMGSWDPSLNAFADIQYLGTEVDNGKPLNDSACVEGFDNVGFVLGTSSSLFNQFILQLNSTSISNFLKSFIGDFLKDLSEDSDDVALYSPNPFYGSEYYQDNITQGTLSKAEVLYLTDGGEDGENIPLVPLLQQEREVDVIFALDNSADTESSWPAGLSLISTYERQFGMLGKDMAFPYVPDEETFINAGLTDKPTFFGCNASNMTSLEYIPPLIVYVPNKQYSFASNTSTFKMSYSDSERLKMIKNGFEAMSRNNLTEDSNFDMCISCAIIRRSQERLGLSTPKECQQCFDTYCWDGKRDETALSEENSLANSSDSSSISFITTSGSASATGGSSSSSSSSSASASSTSSKASSKNAGSSSTSVTFSQLFAFVISITLGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE61</accession>
    <name>A0A1E5RE61_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Chromatin assembly factor 1 subunit p60</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1688</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat HIR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE61">
        <property type="interactions" value="836"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE61"/>
    <dbReference type="InParanoid" id="A0A1E5RE61"/>
    <dbReference type="OrthoDB" id="71227at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033186">
        <property type="term" value="C:CAF-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055410">
        <property type="entry name" value="Beta-prop_CAF1B_HIR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045145">
        <property type="entry name" value="PTHR15271"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15271">
        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15271:SF4">
        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24105">
        <property type="entry name" value="Beta-prop_CAF1B_HIR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CAF1B/HIR1 beta-propeller" evidence="10">
        <location>
            <begin position="71"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="165"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="484" mass="53907" checksum="AD943E68A9524FBE" modified="2017-01-18" version="1">MSIEAFPIVAYWHATKPVYSITFESGSLNRFITCGGDSKVKIWKFKTDSASEENGNTPPHQLSGKTFTQLAQESVQKIEFLSSLVGFEKTVNVGSFSHSSSESCKNNINVLACAGDDGXIMLWKKETSEDIKNGVKHSSIAMMAHDEFDDSSDNKENWYIWKKLRSTNTSEIYDISWSPDNEYLVAGCLDYSVKIFHVQSERCLYHSTSEKGHSNYVQGVEWDPLNELVITLGADRAMCVYKLQWNNKTGTLKDLKLINKIIKGDIAGKSNFLFKNVDTSFFRRLKFSPCGSILLAPAGIWKQTPTDGSQVLPDQPNESXSNCVHIFSRANLLQKSSKPIGTIPNLNSRAVAVAFSPNFYKIASQEDSLFNFAYRLVFAVATLDQVLIYETDKLSCVAVVRNLHYSSIHDLAWNSDGSRILVSANDGFVSTIDLSKFAPRFGEILPNKSDFVKPFVPASAKSCSQLKSKPVSQPAVNVLVPRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE95</accession>
    <name>A0A1E5RE95_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative peptide chain release factor-like protein, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1990</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE95">
        <property type="interactions" value="58"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE95"/>
    <dbReference type="InParanoid" id="A0A1E5RE95"/>
    <dbReference type="OrthoDB" id="277888at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000065">
        <property type="entry name" value="Peptidyl-tRNA hydrolase domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052405">
        <property type="entry name" value="Mito_Transl_Release_Factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46203:SF1">
        <property type="entry name" value="MITOCHONDRIAL TRANSLATION RELEASE FACTOR IN RESCUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46203">
        <property type="entry name" value="PROBABLE PEPTIDE CHAIN RELEASE FACTOR C12ORF65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="Release factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Prokaryotic-type class I peptide chain release factors" evidence="6">
        <location>
            <begin position="47"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="129"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="129"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="141"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="169" mass="19520" checksum="726914FDF06D7584" modified="2017-01-18" version="1">MIKLLQXLPIHSKPLLVANKSAFHTLNTLSIKAHELPPRPKFTPEMESEIEESFLHGGRGPGGQKINKCNSKVQLKHVPSGIVVNCQETRSRDKNRKIAREKLAMXIAVWKNGGQMVDRQLKMIELKQQRKSSKLKKSTKKHELLKQQKEEQELKQEQEDREFLAKMMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REE9</accession>
    <name>A0A1E5REE9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative transporter SLY41</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2105</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REE9">
        <property type="interactions" value="554"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5REE9"/>
    <dbReference type="OrthoDB" id="1588579at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="462"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="516"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="7">
        <location>
            <begin position="164"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="7">
        <location>
            <begin position="413"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ85288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="540" mass="60879" checksum="275D83332637F93B" modified="2017-01-18" version="1">MQMITIHHEQGQQRREAGSSTGDIQQHPWQQXHAETSNNGIKRRLSLTVKGHAHRKSVYDAAAXNAQFAFPXGTAYSSQHSEAIPTGREEELHSTFFNLPTKAEGIDYQNYYSNEKDQMEKXNFFLSTSEKSDFLYTQPVEVNKFTGAYDTFIKXYVPDMDSSIIVICTVWYICSSTSSNLTKHILKEFPHPIALTELQFCVSALISVLFIISLNLMSNNKKFPILKLFPPGLLPEYLDGGFRSSILHGFLQFDKKILMSTLPMGMFQFFGHITSHKSTRLIPVSVVHSIKAMSPIITVAYYRVVHNRIYKILTYLTLVPLIGGVIMTCVSSSSSKKKSTAAQSNASGLGGYFWGLNYAFVSMLIFVSQNIFAKNVLTLKKGSADTGILGEGDATAQMRXKRNQMLNHKNSQQKNHKKHTSNIDKVTISFYCSCVGFLIDMPLFIYNEFTSRALYQDLKPNILFLVIIHGISHFIQTILAFQLIGLLSPVNYSIANIMKRICVILVALFWENQWSANQVLGIILTICGLYSYDKWGKTTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RER0</accession>
    <name>A0A1E5RER0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RER0"/>
    <dbReference type="InParanoid" id="A0A1E5RER0"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="425"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="437"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="458" mass="52413" checksum="63F80D190975E0C4" modified="2017-01-18" version="1">MIDWETRLACASESDESLEEMSKSVTSSIEFEQTHYLKVRKFFEQKVQQAKEESWNANCTRELNILAKSPVHDENFESNNDKQFNFGNDNEFDLSSILDCYSEKSVNSLEKVARQGSRVFEYQKANYVVLPGSGNDKSKEELSCLTNKNDNQSTTIAQLALQTPGLGVQSNISRQEKFGRSEGGEIIDLGRLVFHQECPHISHKSSSSEIVKQKLNSNFPVTKCLVRPNKRTNLDQNEHQKCVFRVFSDKRKPRIVSIMEKTDSSQDDTDDSDIVSVSQQTFFHQNQCINQTKPINQSEYVQYRFNTGNGRLYSGYYDNASLSNCDYHTDQYAKMVPLAKLPSPMSFEKSFDDFESNIFDRENHNMSNNTTNTMTTTVVDDKQFSSTSDKFMSNGNLLRTGQTNFISLNDTPDYLDQSKYQHSMSNKNVQNSNMKQNKSKRLAHKLKQAFSRTSAARI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF13</accession>
    <name>A0A1E5RF13_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UPF0642 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2134</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF13"/>
    <dbReference type="FunCoup" id="A0A1E5RF13">
        <property type="interactions" value="97"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF13"/>
    <dbReference type="InParanoid" id="A0A1E5RF13"/>
    <dbReference type="OrthoDB" id="4087970at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019434">
        <property type="entry name" value="DUF2423"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28219">
        <property type="entry name" value="UPF0642 PROTEIN YBL028C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28219:SF1">
        <property type="entry name" value="UPF0642 PROTEIN YBL028C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10338">
        <property type="entry name" value="YBL028C_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2423" evidence="2">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="142" mass="16054" checksum="507AB12F12BF1720" modified="2017-01-18" version="1">MAKSLRASSTIKAKAFRKNNLEYQKYADSRVLRISEKLKQNYINEKVKKYLKEHEGLETVDEDIMAQFAAEAEKLIGSKSQPRVDGNGAQDIDSMDTDGATDGDKSAAKSISTSGWRDARHLNYRKAKKMKKSKKRGSFTKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF18</accession>
    <name>A0A1E5RF18_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Bud site selection protein 20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1961</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ZNF593/BUD20 C2H2-type zinc-finger protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF18"/>
    <dbReference type="FunCoup" id="A0A1E5RF18">
        <property type="interactions" value="442"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF18"/>
    <dbReference type="InParanoid" id="A0A1E5RF18"/>
    <dbReference type="OrthoDB" id="24683at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000299">
        <property type="entry name" value="Zinc finger protein 593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051879">
        <property type="entry name" value="C2H2-ZF_Maturation_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46095">
        <property type="entry name" value="ZINC FINGER PROTEIN 593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46095:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN 593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="155"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ85474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="190" mass="21769" checksum="8A1CEC6927E62F1C" modified="2017-01-18" version="1">MGRYSVKRYKTKRMVKSLDLIFDELTDPSKVAKLLNQPLDETKPGLGQHYCIHCAKYFETAEALKTHLKSKIHKRRIKDLKGVPYTQETSNAASGLDVQKFLNKVQEIQTVKGPNKEAIEKELKTHLDATLENAKAIDQMTYIDKVNNQENGISANIPVQNTAGPVEQQQQQQQQQQQQQQQQQQQQQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF25</accession>
    <name>A0A1E5RF25_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mitochondrial import inner membrane translocase subunit TIM23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1725</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion inner membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Tim17/Tim22/Tim23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RF25">
        <property type="interactions" value="584"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF25"/>
    <dbReference type="InParanoid" id="A0A1E5RF25"/>
    <dbReference type="OrthoDB" id="159299at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005681">
        <property type="entry name" value="Tim23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045238">
        <property type="entry name" value="Tim23-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00983">
        <property type="entry name" value="3a0801s02tim23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15371:SF0">
        <property type="entry name" value="SD19278P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15371">
        <property type="entry name" value="TIM23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="227" mass="24692" checksum="DB799339D6AFDCB2" modified="2017-01-18" version="1">MSWILNFGKKPQQQQQQQQDQQQNIPTSVNDTNSINNTNTXDERKQLNQTLGFDQQKLNTQTKSLHDFANLDKGVESLDLDEEKLSNLEGSQGLIPSRGWNDDLCYGTGAVYLSGLGVGGLYGFFEGLSKIPPNAPGKLQLNTLLNNITKRGPFLGNNAGVMALSYNIFNSSLDYYRGKHDHANSIAAGFLSGALFKSTKGLKPMGYAGGLTALAAAGWCGLKEYLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF26</accession>
    <name>A0A1E5RF26_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endoribonuclease YSH1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Endoribonuclease ysh1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 11">mRNA 3'-end-processing protein YSH1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g1992</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF2/YSH1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RF26">
        <property type="interactions" value="1017"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF26"/>
    <dbReference type="InParanoid" id="A0A1E5RF26"/>
    <dbReference type="OrthoDB" id="10249535at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16292">
        <property type="entry name" value="CPSF3-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000001">
        <property type="entry name" value="Cleavage and polyadenylation specificity factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10890:FF:000001">
        <property type="entry name" value="Cleavage and polyadenylation specificity factor subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021718">
        <property type="entry name" value="CPSF73-100_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050698">
        <property type="entry name" value="MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203:SF11">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11718">
        <property type="entry name" value="CPSF73-100_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16661">
        <property type="entry name" value="Lactamase_B_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01098">
        <property type="entry name" value="CPSF73-100_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="13">
        <location>
            <begin position="27"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-Casp" evidence="14">
        <location>
            <begin position="256"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA 3'-end-processing endonuclease polyadenylation factor C-term" evidence="15">
        <location>
            <begin position="553"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="504"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00075008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ85484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="823" mass="92459" checksum="2A2364312A925794" modified="2017-01-18" version="1">MSILVSPEATSDSSFKFFGLGGSNEVGRSCHIIQYKGKTIMLDAGVHPAYRGMASLPFYDEFDLSTVDILLVSHFHLDHAASLPYVMKKTTFQGRVFMTHPTKAIYRWLMKDFVKVTNISERAVNDYDESNLYSNADLADSFDKIETIDYHSTIEVNGIKFTAFHAGHVLGAAMFQIDIAGVRILFTGDYSREIDRHLNSAEVPPLSSDVLIVESTFGTATHESRHTREKKLTSLIHNTVSKGGRVLLPVFALGRAQELLLILDEYWTQHAEELGHGQVPIYYASNLARKCLNVFQTYVNMMNDDIRKKFRDSQTNPFIFKNISYLKNLESFQDYGPSVMLASPGMLQSGISREVLEKWCPEQKNLVLVTGYSVEGTMAKFLMLQPDTIPSYNNPDVNISRKCKVDEISFAAHVDFRENLEFIEQINAKEIILVHGESSPMGRLKSALLSNFKDLKNTKNQVHVHNPKNCVLVEVEFHGIKIAKAIGNIVDEIKGQLIELKDESQDEDVKSEEISEGETTEKLEAQKDNLKQEGATNEIESKVHINEEGLVEEDTGISVSGIVVSDESHFDLNLVSLSDIRTHHSDLKTTILKERQSLKISCKKELIYWHLCQMFGDLKVLVDDDNISNTKEDEPKDSAKTNDDSGEFEVQIMNSIKVTIYNSIVTVEWVQNALNDTIADSVIAVLLGVESSPVSVKMSSKSCQHDHSLDHSHDKVCQKTDFEGDEKMTHDQTSVKDAEDEALWKVTEMARLFXEQFGDTFKLXLDKEEYKVGDEIVGTVSLGQXVAKINFSKMNVEDCPSKPLKGRIETILQIASDLLTPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF61</accession>
    <name>A0A1E5RF61_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1806</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RF61"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="112"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="251" mass="28693" checksum="474C6097AB4EA259" modified="2017-01-18" version="1">MNPAHPVHPVPXPDDPPPPYPNDIDGAFTLRVDRSXPRVLRHXSGPSPYTQDXQSVTGPXQDTERRLGKRQRSLLKVATYKKKFKDMFSAQQDPAXTSASRTLRTFTRETSRTPSSTPRPTGPTNTAGSTHAVRNQRSGTQLSRPTAGSPARRRYVRTFKWDFFCKKLXWRSRTSERLSWKSADLFLQALANSLWEDAQRHHKNAQGHSGVRNRNFALRYKTFAAFSVLLLVWFYLKAADHAVQWIRNLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFD0</accession>
    <name>A0A1E5RFD0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MoaB/Mog domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1865</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFD0">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFD0"/>
    <dbReference type="InParanoid" id="A0A1E5RFD0"/>
    <dbReference type="OrthoDB" id="448496at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047884">
        <property type="term" value="F:FAD diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042726">
        <property type="term" value="P:flavin-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47675">
        <property type="entry name" value="MOLYBDOPTERIN BINDING DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G11210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47675:SF1">
        <property type="entry name" value="MOLYBDOPTERIN BINDING DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G11210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="2">
        <location>
            <begin position="31"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="311" mass="35198" checksum="6A2BDD0FCB7349ED" modified="2017-01-18" version="1">MLRAATQQARLLSKTTRKMAEQSLNPPINAACLIIGDEVLNGKINDTNSKYFARYCFDLGIHLTEICVIGDEEEQIIETATRLAQKNKFIITSGGIGPTHDDITYESLAKVYPDTPNLLSQECVDKMEKIHGSSKKMSGEALKNHYRMATLPSGPKCKHYYLDDKMWVPVVSIYDQFYILPGIPQLFEKLLQLLKPKVKEIYGLKEMKYVRYFVKTPFGESAMSEFLRNLQEKANXKSTEIKIGSYPHWGLGFNTISILGTDSQDDFLQKLRDECIAELQGTEISAEDEEKVSDTRKDPSDDGNAKLLLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFF9</accession>
    <name>A0A1E5RFF9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Centromere-binding protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2185</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFF9"/>
    <dbReference type="STRING" id="56408.A0A1E5RFF9"/>
    <dbReference type="InParanoid" id="A0A1E5RFF9"/>
    <dbReference type="OrthoDB" id="71302at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47787">
        <property type="entry name" value="CENTROMERE-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47787:SF1">
        <property type="entry name" value="CENTROMERE-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="3">
        <location>
            <begin position="221"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="304"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="43"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="119"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="167"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="367" mass="41017" checksum="BE83C04CD546B57F" modified="2017-01-18" version="1">MPESYKKSNIENEHPEIDPAVDTALTEQSTSDQENDQQHADGEEQQEGQEKEDEEENTTTTRYHLHNHRPSENNDSYSFESKKRRSSDSSESSSKSKKSKKSKDNEPKKEDEEEDEHTTTTTTTNNSDDTPNPIITITSTATAAQAVKAAQDASNDDDYTTKTSTTAEEDDEGNDEGAENDGKKKKSGRGQSAKDKNKKNRIAAIVGALDDDAMEDIKLQQRRESHKEVERRRRENINTAINKLNDLVTLSIEKSAASSASSASSSSNTNSTSNVFQNGEDEDTKDKKDSKKGTQTANPKAKILLRACKYIESLNKELEDLREKSNLTKLLDEQELTRLSSENENLKTALEEAYKKLKKSGIKEDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFJ4</accession>
    <name>A0A1E5RFJ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Mannan polymerase I complex VAN1 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1671</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ANP1/MMN9/VAN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFJ4">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFJ4"/>
    <dbReference type="InParanoid" id="A0A1E5RFJ4"/>
    <dbReference type="OrthoDB" id="204164at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00761">
        <property type="entry name" value="Glyco_tranf_GTA_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000163">
        <property type="entry name" value="Van1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052086">
        <property type="entry name" value="Mannan_Polymerase_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083:SF5">
        <property type="entry name" value="MANNAN POLYMERASE I COMPLEX VAN1 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083">
        <property type="entry name" value="MANNAN POLYMERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03452">
        <property type="entry name" value="Anp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="546" mass="62884" checksum="5819E68C3FC8CE99" modified="2017-01-18" version="1">MDSNSLPKYHTYNSSSSSLNGNDSAYDQDGPSYKKHWNSKKSESKLKNMKXKATEFMFNFKDLKLRNLKNNSLSRKRFIIALIKLVFILVTGGFIFQKLIWEQLAEVGSFTEFFTDFFNEDAVTQYNSFASLDDPEYYNFDFRNLDVDTVSKFDKGAKHFLITHEAQIITDQDKKTLEAELEMLLATSVQKYDLRDYDGSPEGAGKREHVLLCVPLRNAEPVLPLMFKHLMNLTYPHELIDLAFLVSDCSPDDKTLETLLDYSKELQNGTLSSVLSKIPHSPVDEMHLKYMNQDYLNRVKTAFEPPFHKSYNKPFRSIQVFQKDFGQVIGQGFSDRHAVKVQGVRRKLMGRARNWLATNALKPYHSWIYWRDVDIELSPGTVIQDLMSFDYDVVVPNVWRPLPVFLGSEQPYDLNSWIESPPALELAKTLDEDDVIVEGYAEYPTWRVHLANLRNPNGNTRELINLDGVGGVSILAKAKLFRKGINFPAFTFENHAETEAFGKMARKMGFRVGGLPHYTLWHIYEPSEDDLKEMASRDRDERNNGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFX9</accession>
    <name>A0A1E5RFX9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BEN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RFX9"/>
    <dbReference type="OrthoDB" id="4096434at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="216"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="600" mass="66141" checksum="C6BCA02097EE75A5" modified="2017-01-18" version="1">MLDNIPLNTNTEDIFISFFSNTCNAGDNGVCHVKETSVESEQNGNVSGSATPCVSASPQCINNTSSTTTTNNSNGLVDESHDCNNINNGITSYEDDTDFSLAPSSIIDHHNQFNHYTHLDVQTPSISYDSIQEVGNLDIFEIVGKDHVNDQKPNGAVYNNNTDTLQETILLQPVNSLSTYTSNASTTPLSNSIMEDDMLYSYNNSFPYNATTHPFSKSSISQPPPSSTASTVSSTFDLQSSRMTSFSSGSSVSLSSPFQYKDIYDNHETNTNISTLSMYDIVPMHILMQNXTTTXKITKTHQIYHNSHTTNGXNLQSDXSTNYKNNHTMQNNNGQLHPLMNTYLPTNATTGTTTAFSAPRNVMGGECDTVAANANYDCLTPGVIPSTSNFKAKANFPNVVLMTSPQTKKKQRKTNKTINENNAGHGIDGLDPMCNNDVVFSSDINDGVGCGSTTPSSSISPASLSLIKTVGHAVYTKDKKISDSRLSAEALARVLNLQSADEAREIEKFVLECLSQKCGFPLGYKTWVRDTTKSERDVILGKLYLMIKPRYPQYSIEILEKIIRRATYYMMQSRLRRERRAKAKAQKQELLQQERALNNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGD8</accession>
    <name>A0A1E5RGD8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1523</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ85988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGD8"/>
    <dbReference type="FunCoup" id="A0A1E5RGD8">
        <property type="interactions" value="282"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RGD8"/>
    <dbReference type="OrthoDB" id="1588579at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="268"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="313"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="6">
        <location>
            <begin position="9"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="6">
        <location>
            <begin position="259"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="386" mass="43559" checksum="6A48C1982225F954" modified="2017-01-18" version="1">MKINSFAFRIVTTCLCWYGISSVGSQITKRILSECPMPLLLGEFQFIYIALLAMMTSTAAYYCPQFYNAFPHGTFPDSYKNKPNGRFTHIITRPTKHIIITVLPLGVFQFVGKYFGHKATALVPVSTVSSIKTLSPLFILLVQRMFRIQSKITLHMVISLCCIICGVYSIVYFDANSLKKSQVSALAGDTALDNIKFSSGIIAATISMLVFVFQNIYGKKVFTYDEKPALDDYHSAKKETLPVYQAPTTKWYKTPLLLSTKKYDKMTLMIYISLFGFCSSFLWFVALELPLLWQPKTLDNTVLESVAVPWKLLFVNGTLHFIQAMIAYYLLGQISTLSYSISNLMKRIIIIVFSWAISSGSSSFTVIQFVGLFLNALGLFLYNRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGJ6</accession>
    <name>A0A1E5RGJ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphatidylinositol transfer protein PDR17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1573</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGJ6"/>
    <dbReference type="STRING" id="56408.A0A1E5RGJ6"/>
    <dbReference type="InParanoid" id="A0A1E5RGJ6"/>
    <dbReference type="OrthoDB" id="75724at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052578">
        <property type="entry name" value="PI_Transfer_CRAL-TRIO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45824">
        <property type="entry name" value="GH16843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45824:SF5">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN PDR17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="197"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="397" mass="45861" checksum="A549CFE5A73849D7" modified="2017-01-18" version="1">MGLFSRLTGSGSNHQKSEHVSPVSSGDKISTDAKTNQTSAQSSSSASSSSNNEVVSSGNKTKADGDKLIECDTMITKPPQSYYDLLPKLLKEHEETKLTSEQEAMYEKLHSLLTDPEFKLPVKNCTKWEEEPKEEWIPLTSWEKFYLTKECLLRFLVGCKWHFDNVKKVLLETLVWRREFGLTYDEENFKNCIQPEDLSEENETGKQILLGFDKKGHVLLYMKDGLQNTDSSLNKVKHMIYMIEQAVLWNPKGVSKVVILVDFKHYPEVPGVLKKRKMTPLSIAKQCLHICQTYYPETLGYAVFINIPWLGWTFLKLMVPFVDPNTKKRFVFDEPFTNYIDPNNLEAAYGGNLDFHYNHKKYWPDFIEKSRERRINNYNKFLELGGCVGVSEYDIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGN4</accession>
    <name>A0A1E5RGN4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Flap endonuclease 1</fullName>
            <shortName evidence="15">FEN-1</shortName>
            <ecNumber evidence="15">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Flap structure-specific endonuclease 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AWRI3579_g1369</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="15">Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Nucleus</location>
            <location evidence="15">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Nucleus</location>
            <location evidence="15">Nucleoplasm</location>
        </subcellularLocation>
        <text evidence="15">Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14 15">Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="15"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGN4">
        <property type="interactions" value="1059"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGN4"/>
    <dbReference type="InParanoid" id="A0A1E5RGN4"/>
    <dbReference type="OrthoDB" id="1937206at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09907">
        <property type="entry name" value="H3TH_FEN1-Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09867">
        <property type="entry name" value="PIN_FEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000009">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000003">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00614">
        <property type="entry name" value="Fen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023426">
        <property type="entry name" value="Flap_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF9">
        <property type="entry name" value="FLAP ENDONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00842">
        <property type="entry name" value="XPG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 15" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6 15 20" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9 15" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 15" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 15" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="19">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="18">
        <location>
            <begin position="148"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="360"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="102"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OEJ86055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="385" mass="44117" checksum="FDF740612B18ED5C" modified="2017-01-18" version="1">MGIKGLNAIISENVPHAIRKSEIKNFFGRKVAIDASMSLYQFLIAVRQQDGMNLSNAEGETTSHLMGMFYRTLRMIDNGIKPVYVFDGKPPVLKKYELDKRLDRRTDVMTKLEDLKDNAEKQEEYNKLQRRSVKVTKQQNDEAKHLLKLMGIPYVEAPCEAEAQCAELAKRGKVYAAASEDMDTLCYKTPFLLRHLTFSEQRKEPIHEIDTEKILTGLDLTLNQFVDLCILLGCDYCPTIKGCGPVNALKLIKEHKSIENIIACIEDPEKNAGINKKWAIPQNWDYKEARELFLNPDIVNADTIDLKWEPPNEQELLKFMCEQNGFAEDRIKSGIERLKKGLKTGTQGRLDGFFKVIPKTKEQMQKKNGKLNKKEPKGKVXKKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGV6</accession>
    <name>A0A1E5RGV6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Vacuolar amino acid transporter 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1584</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGV6"/>
    <dbReference type="FunCoup" id="A0A1E5RGV6">
        <property type="interactions" value="125"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGV6"/>
    <dbReference type="InParanoid" id="A0A1E5RGV6"/>
    <dbReference type="OrthoDB" id="28208at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF458">
        <property type="entry name" value="SODIUM-COUPLED NEUTRAL AMINO ACID TRANSPORTER 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="194"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="296"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="340"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="386"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="416"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="448"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="10">
        <location>
            <begin position="74"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ86096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="481" mass="53110" checksum="5075867F28295825" modified="2017-01-18" version="1">MSSTKDKYHKLDNGKALELSSYDASPSTHPLPGEQTHAKNELDIDNDDFEMNTGESFKWADGDSNEYLDSHNSRSSFLMGFMNMSNSIIGAGCIGLPMAIKYCGIVGGVLVLVFSTLLVDFTLRLVVVNLKLSGTSTYQDSVEAAMGKWGKLLILLGNSFFAIGGCIGFCIIIGDTIPHVLKAFFPSHASIFHRNIIIVLITIFISYPLSLNRDISKLAKTSLLALLSMGLILISVIIRGPLTDSSYKGEFVFPNYLISSKLLQGMSIINFAMICHHNTSMIFSSLRKPTLKRFDTLTHLCCGIAMVVCALMGFTGFAIFRDKTKGNILNNFPSDDKLINVARFCFGFNMLTTYPMEVFVFRECIRDLIFFKNYDLREPPVLSKKLHFLITTVIVLVTMSISLTTCNLGATLEIVGSTSGSLMAFILPPWVNLIFTGKEKPPKEKIKYYFTIGVGIFLMIFSSLQTLIDAMKGSADSHCSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGZ8</accession>
    <name>A0A1E5RGZ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dolichol kinase</fullName>
            <ecNumber evidence="3">2.7.1.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1303</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the polyprenol kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.108" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGZ8"/>
    <dbReference type="FunCoup" id="A0A1E5RGZ8">
        <property type="interactions" value="86"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGZ8"/>
    <dbReference type="InParanoid" id="A0A1E5RGZ8"/>
    <dbReference type="OrthoDB" id="377083at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004168">
        <property type="term" value="F:dolichol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043048">
        <property type="term" value="P:dolichyl monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032974">
        <property type="entry name" value="Polypren_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13205:SF15">
        <property type="entry name" value="DOLICHOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13205">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="183"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="211"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="270"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="317"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="347"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="368"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="407"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="467"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ86167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="539" mass="61442" checksum="052858E44D3A0013" modified="2017-01-18" version="1">MAVKNRNNSKSKKSGNAIVPNSNSHSNKETTDQTDAVSEDATIDDDKFDFLTEVLCTNRITQVMIFLATCHLFYFQNENNSQLLSKVFNTLISFVIVFFVYYRRGALDINIDNFEKNYFHLIYLFLLPIGITIYYAHPRMQYLGVNCALVLNYLPVPLHLKLPLQGIFALINSEDAYFYDYKFHYLVAIPLNYCIYFVLTKISQLKSLSVVECNVFAILATNLLYLYQSESVEFQILQKSLWSLILIATVNWLLDLSLFHKSANSASRKIVLGGITLLGFPFLLQTFLSRFQSATFSGNAVQWLYQYIVEDPLKLKIMGIWLASLLILIPNILVMKSKLSLNTSRKLWHFIICLLITIPFKYDPEFVKIGISGVVPLFLLVEYLRYTNLCGNYVNDSLKSFSDYRDSAGPVVISYLYLIIGVSFPLIINNDICGCIVLGVGDSIASIIGKKFGKKYWDGPDNKTMEGTAAFVISTFALSLILKAFYNDWDYLPILSNKDQLFEIFTNCFVAGILEGNATFNDNILLPPFMMIFSKVMKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH85</accession>
    <name>A0A1E5RH85_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2-(3-amino-3-carboxypropyl)histidine synthase subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1215</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2. DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase. Facilitates the reduction of the catalytic iron-sulfur cluster found in the DPH1 subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the DPH1/DPH2 family. DPH2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH85">
        <property type="interactions" value="957"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH85"/>
    <dbReference type="InParanoid" id="A0A1E5RH85"/>
    <dbReference type="OrthoDB" id="449241at2759"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11860:FF:000001">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010014">
        <property type="entry name" value="DHP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00272">
        <property type="entry name" value="DPH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762:SF2">
        <property type="entry name" value="2-(3-AMINO-3-CARBOXYPROPYL)HISTIDINE SYNTHASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01121">
        <property type="entry name" value="Diphthamide_biosynthesis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00408">
        <property type="entry name" value="Diphthamide_biosynthesis_famil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="6 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="550" mass="61664" checksum="CE97F539B27E52A0" modified="2017-01-18" version="1">MSETVAPSLSTFQADYLFQKYEQPHHARPHLNQGSTETSVAQQLNEYYSVSEILDFLITNPQYEKITLQFPDHLVMDSAYILKDIQAYFTSXEDNKRQFWILADTAYSSCCVDEVAAEHVKGDLVIHFGDACLNSVQKLPVAYSFGKPKLSQDVAVQFEKRFPDKNDKVCLMSDSSHCYLLPELSSKLKENGYTNVIYTGIPKDIVPEGSFVANFXQNDKIGSTKTKIGRNRVVFSEHEVTQEELQQDYTLFHITTPADPHLLFLTTSFANVVLYDPSSMETFEGPFPSMMKRYKFMHVARTAGTIGILVNTLSLKNTKETINALTKLIKSHDKKSYLFVVGKPNVAKLANFESIDVWCILGCPQSGIIVDQYNEFYKPVITPYELQLSLQYDVTWTGKWVTQFEDVLKEIDGDMEADENDTIKEDADLKEKSGSTHNDQVDDMPVFSAVTGKYVSTSRPLRHVQHLELDAPTENQTSSNSLVKNFSSHLTIKNTVSTSAAALQNRTWTGLGSDFAGADEGDAEEEGATLQLGIAGVARGYAFDRENTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH99</accession>
    <name>A0A1E5RH99_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Regulatory protein SWI6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1603</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RH99"/>
    <dbReference type="FunCoup" id="A0A1E5RH99">
        <property type="interactions" value="589"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH99"/>
    <dbReference type="InParanoid" id="A0A1E5RH99"/>
    <dbReference type="OrthoDB" id="6718656at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030907">
        <property type="term" value="C:MBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033309">
        <property type="term" value="C:SBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051642">
        <property type="entry name" value="SWI6-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828">
        <property type="entry name" value="ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828:SF3">
        <property type="entry name" value="CHROMO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="324"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="501"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="424"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="532"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="563"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ86254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="724" mass="81546" checksum="A47FE98507155E94" modified="2017-01-18" version="1">MDQEFSRTAPSGETFVITKHEDDGVYHISENLCKVNPNYQLSTNKTILESEYIFKNDLIDYLQSLNLFDLFSDIFIQNNESSKSKTSSFGSPNSGLTMQFTTSSDAVTFTTKQKQALEFFLQRVLAPTNNSDSSRGKELHAKKDHAKVILQNVFQDFKNIYPNEVLNLKIPIDENGSTALHWLGSLANSNLIREIVQYQKETSAMSSAGPNGKVKIEMDISLGDAMGESALVKAVKTVNNYENGTFEGLLEVLYPSLNLLDNQRKTILHHIILQSGKQGCAIAAKYYLDIFMAWVVKPGGVEVDGHTVTLQWFINNVLNAQDVEGDTCLSLASRLGNVAFVNLLLDYGADPAIANNSGLKPLDYGATLRTNDQQKFLNGITDQTNSCDIKFENASSNGSLRVPFDLPNNAPMMDHMAKLIESINATYKEELTSFNNEMEELQKNIHIKNVELNTVRHKIYQYQALKDEYSKLQEQITSLKSGIRDQKKLFQVLSQDFPELLEETGGSGSLEPEESNSAQGTQEIPDYDEFGNSRSASTDGVVKNSESLNSDDNKDEKHTNGSGEEEEEKEEEETFDADEPFKIDFVYSYLSDKLENEFNGDIDKMEQSIDIQQLVEHFMETETTSDEAQSNIEDLPSLSELQFRLKMYEENEVGLDRFYKQVQEEQENLESKFKRVLSLCLKVDESKVDSMLDGLLEAISNEDLENLALDDVQSFLKQNETMIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHH4</accession>
    <name>A0A1E5RHH4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">RING-finger protein MAG2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1233</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHH4">
        <property type="interactions" value="588"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHH4"/>
    <dbReference type="InParanoid" id="A0A1E5RHH4"/>
    <dbReference type="OrthoDB" id="302966at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039739">
        <property type="entry name" value="MAG2/RNF10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12983:SF9">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12983">
        <property type="entry name" value="RING FINGER 10 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="262"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="485"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="648"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="715"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="37"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="90"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="650"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="715"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="746"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="787"/>
            <end position="798"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="809" mass="91326" checksum="CED97EF055FF25A6" modified="2017-01-18" version="1">MDKKNGSSTGIIPSGXSAIXNNNVNGNDNSNSATNVQAYNDNAQNRXNYANNTKSKKKSNKRNTNXRRGSNDTKDSKGIXNSGETLGPAKHFHRNNNKPNRRRNSSTLNTTPDDNLPSNSAFNSYKNIKTKSKXNVLVDPYDFEQDLQDQIMASQFRSGTKKAQISINHLLDLQFDRESNGPHSGYSSSSTSNRNYLKNYRDNDLNSAHLHGASFINATYKFVVLDKHDYSEQYANPNVLIKPEQIIRVVILKDEKEESQNCPICLSSDIVAPRMVACGHVFCYTCLLQFFDANNADTTFAASTSTASLSSATSQMHKPMKKRYHECPLCASMIKENSSNILPVLFETRKSSLHGDTTIVSTDNTLSSNKISVNKQMDFQLICRPHGNILGLPVNLGLDPSLCEYPPRMSSMIDTDNYQFSRIVQTDVLDQIRFYKKDLQSIQEQHQLDQLLYNDDGKYAKLAEQKINDTIEHILSEQLSNLDLKPNSKSSLNNQTKKTSQRSSENAFIGTDTILQKYDDSNAFFYLEHVDXLGKRFFLSSLDVKLLKKTFGAYSKFPLSLHAKCENFTIDQRVNEYSIQNFKYFGHLPMGDTFNLCDIDWNYTNNHYNALQTQKNLETLGETGNASDVTEPTNESLIPSAVMENFKTQLKSRRSKTTRKKQKEDHEKKVYEKQLEIKHHKFYEQEGAGSASNWALDTNNIPALXNTFSSSVEHFPDLPRHDAGLSNSDHSDGENADATNKKREYTQTVWGTTIPKNEQNQDSGSDQDSWLPQNILDQIDSLNTKQKNGKGKKGKKKITLFANSQRGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHH8</accession>
    <name>A0A1E5RHH8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">AP-1 accessory protein LAA1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1540</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHH8">
        <property type="interactions" value="122"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHH8"/>
    <dbReference type="InParanoid" id="A0A1E5RHH8"/>
    <dbReference type="OrthoDB" id="192608at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040108">
        <property type="entry name" value="Laa1/Sip1/HEATR5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046837">
        <property type="entry name" value="Laa1/Sip1/HEATR5-like_HEAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21663:SF0">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21663">
        <property type="entry name" value="HYPOTHETICAL HEAT DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20210">
        <property type="entry name" value="Laa1_Sip1_HTR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ86335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1849" mass="213262" checksum="BA3077C62C5E2B6A" modified="2017-01-18" version="1">MPDPISFIANLENSVDKAQSIAIWLCKQDETFALNTKTLEDDDKWDCEEQCINFYNSMFELLKYFVKKEYYHESFTNGDGSIQEKDTSFLSPQLQLQYAQVLRNGLLQLYQYMPGKINDISMILANIFCEEEIPRVSIDDGKKKKKKKLTIKKQYHFSELKSLCCVCLDFLIEGFKNNLTSLFPLIQTGLFRNLKKMKEKPKHFHANFSINLIKLFKSIVHNSPISKEFQTKFIKYFRDMSKFPQQFKCTAVECSYILFVNHKSLRNAALMEELFKALEVDLYFDISTNATSKMICDVLVFYLTENRITMEECLEFYCKLFTNYPSRIVKAGVFESIIQFLTINYMKDPQIFLNEKYLFMLELLVTQIFQHPNIQQRKIDSITRYINYFHQFHEILLVHVSESSKILILRSLFASNKYLKADIEHVNETLLFLDFTKQLLESLLESINIDEQFINTFKNTLLQLSKCEIFLIRIHANVVLKSFFRVYPQNLQATLQSALNSLNSTFSMISDFNFSENHGNALLIANMMSISDPTFVSNDLVLKITVFATSFLKKQNASVGTLSYYKDLLCWIMLTGLMNYKDKSFIQLQKNQLFLFWKNLLTHSITFNTEDELYKNLEIRNHALTCLLAFLKNHKALLDTELIKQVTYLLTKSTAFNNQVNIKSKTIDNSLLHNEHRILQIYAVIKDYVKKDFNSSMLILVLKNFSDPHLFLSKQSNILDEFLKTDKKKAVETAPKVTLNSLLASEDSFAFGLTSLITENSIVSLQKNSKRFIFEWKRNIYWDSKFEEDLHRPCIPVFSYDYMIQLYGSEYDGENAYLPNIIGSLVNHSMFVFSEIFPYLNSSIQLSIMENLNSFMFSKKSVPERLLAIAINSSIAINDAVKVFLTHNLSMDLSVAHLLIEMLKKIVIEGEEQLMKINADTLGMITYLLENQAQKEIIDVSFKAISEQTLPYERGFHLLNIASISKYSPKNIDISRVVDLISNLVSEKDPVVHTWALDALLLTLNKIDSDQNELFSDVVDLLYTVFFSSEYGRNSNFLSIVDYAFKFDTHLIIANIIKRLTQITPPESLSEIDEVCLHKYTTLLHTLSVDIDVNKKCILLETFQHLVTLDSNKISTEVMVQVGVKVLNNSYYAVNSCSNSSLYSSKNVFDWKTSVDAIESAELLFSSLYHCKNFANKEVEYYTFLLLSKYPHNKSLLAFIDRFVDKTWIDKVITFYDMSQERLSQGINNNFVDHLKGLNIEVSATNTESSTIWQFKCVLLKIMLRLVKEQTAADEEHMAKLLELGVSASNSKIKNMKLLGIEIVELLIHKAAQIAKTREADVISVLMSAFDEFSSVQVVNAALTCCSEFFAYYPKVGPENDLIKTLVNSLLEMQNVNQHNLKIGNYVAKTRQAKNDLQMHILSAWATLTVGENFALVENYMDGLIPLWIVSLRQNAVNRHETERDFYSEPLSDVYFKMIQAIGTVANRAKGKLDCFLKSQDLQSFLFVLYAECMEYLIRNKSNKSGRNDTLLTLKHLLQSEIACDLFFEENINNECMIVFDQILMEFDDTDLLVCEICTLLAKNYIRHFQDNRFLEKLDILYELFRIELKVIAKYIPFIEEGVAEDKSGFLQSHQFEVVKEVYTMLSQTCLMLPEEFKDDLTCCLLFTIGMIFNHASCNNYVPYVLPAVKLLTSNNGTLAHELYMCTKNSLEQNFSLQNYLSSMTVFFGNGLDHTSVETFCSRLCQFLIERNEIGDKILNTLLSSEYPMKNLIIKTLLKSLFKEAECAENVNSVIKLFLTVVGDLHSKNSFMLTLLFFNKLKKHHADIDINEYIERVASSTTDKKMLQDAISGLPKDSRHEVCEMLASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHZ8</accession>
    <name>A0A1E5RHZ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2 6">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1639</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FKBP-type PPIase family. FKBP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2 6"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHZ8"/>
    <dbReference type="FunCoup" id="A0A1E5RHZ8">
        <property type="interactions" value="561"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHZ8"/>
    <dbReference type="InParanoid" id="A0A1E5RHZ8"/>
    <dbReference type="OrthoDB" id="1902587at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000006">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044609">
        <property type="entry name" value="FKBP2/11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45779">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45779:SF7">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0697">Rotamase</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184750" description="peptidylprolyl isomerase" evidence="7">
        <location>
            <begin position="19"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="8">
        <location>
            <begin position="44"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="138" mass="14993" checksum="147FF46F60B6DCF5" modified="2017-01-18" version="1">MLKKFVAAFLWLAAVVIAGELTELSIDVTKEIPASECKVKASKGDVVSVHYTGKLLSNEKVFDSSINRGVPIEFVLGYGQVIEGWEKGILGMCIGEERNLYIPSSMAYGKRGAGGVIPADADLLFETELVSLRRNDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI30</accession>
    <name>A0A1E5RI30_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Protein transport protein SEC61</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g1292</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RI30">
        <property type="interactions" value="991"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI30"/>
    <dbReference type="InParanoid" id="A0A1E5RI30"/>
    <dbReference type="OrthoDB" id="420669at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3370.10:FF:000002">
        <property type="entry name" value="Transport Sec61 subunit alpha isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="entry name" value="SecY subunit domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030659">
        <property type="entry name" value="SecY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019561">
        <property type="entry name" value="Translocon_Sec61/SecY_plug_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00967">
        <property type="entry name" value="3a0501s007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006341">
        <property type="entry name" value="PRK08568.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="SECY/SEC61-ALPHA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10559">
        <property type="entry name" value="Plug_translocon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="Preprotein translocase SecY subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00755">
        <property type="entry name" value="SECY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00756">
        <property type="entry name" value="SECY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="118"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="368"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Translocon Sec61/SecY plug" evidence="13">
        <location>
            <begin position="43"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF10559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ86550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="485" mass="53089" checksum="A8C7C7AD741961B5" modified="2017-01-18" version="1">MSSATXQFLEFFKPFQPYLPEVVAPTRAVPYKQKLIWTGVTLLIFLIMSEIPLYGILSSESADPLYWLRAMLASNRGTLMELGVSPIITSSMIFQFLQGTQLLSINKENKKDRELFEVAQKVCSIILTFGQAVVVVLSGNYGKPSDLGIAICLLQCFQLIFASLIVFLLDELLTKGYGLGSGISLFTATNIAEQIFWKAFAPTTVQTGRGTEFEGAVIAFFHLMIVRKDKKRALVEAFYRPNLPNMFQVLATVAIFLFVLYLQGFRYEIPIKSTKVRGQVGVYPVKLFYTSNTPIMLQSALTSNIFLISQLLFNKFPSNPVIRLLGVWGVQNAGAGSNGQKAAVSGIAYYLQPPFSVTEALMDPIKTAVYIVFVLGCCAMFSTTWTEISGTSAKDIARQFKDQGMVINGQRESNVYRTLKTIIPTAAAFGGACIGALSVGSDLLGTLGSGTSILMASTTIYGYYEIAAKEGGFTKNLVSGFSELM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI35</accession>
    <name>A0A1E5RI35_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Mitochondrial inner membrane i-AAA protease complex subunit MGR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1637</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MGR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI35"/>
    <dbReference type="FunCoup" id="A0A1E5RI35">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RI35"/>
    <dbReference type="OrthoDB" id="4087899at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013911">
        <property type="entry name" value="i-AAA_Mgr1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08602">
        <property type="entry name" value="Mgr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="375"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="455"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="19"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="383"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="461"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="486" mass="54741" checksum="59436101671E828E" modified="2017-01-18" version="1">MGLYSPPDKNPGSSKLREGNSSNGNSDGNSSGSNDNNNNNNNNRDNNSSAQNPMLFTSRPSVGLRLWGPLVPASDCTWGMWTLLGIQTAIGFTLLTKFRVSLWKYRMGKTVTKEIADIPSLNRFSTTHGTIQYKPENLLKQVNGTAPVAKNGLNQFGGTHTFHFIHQPTRWEKLVGKWKNSMYSRWMSHSMRLVYLFSGSILLSLSMLEFSRLVVFDYDPWVEQMKSAREKQFYNDTSRFYREGVDPSKFEVKVQDAATGKFVDVTSDPEIRAHVAMARALMVQNSWISKWFGPLEVKPLTFKEYLDKIEQYEVSKLAAEEAFKLIDARLAMPLPEAVQHLNTFALDSENTRKKTLELLHSLPANVLPSVSSIYANNPLNDQPDGKGAERKTLGQAPKEKHNSLQLQDDIKIFPPNCKEPQQVRVGDTWLYNDPWSKLGVETHLGVKIIPSTPTYFEETNDGTNTTNQDSTLESSGNFTINNSKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI45</accession>
    <name>A0A1E5RI45_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PSP1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1609</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI45"/>
    <dbReference type="InParanoid" id="A0A1E5RI45"/>
    <dbReference type="OrthoDB" id="243127at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047767">
        <property type="entry name" value="PSP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007557">
        <property type="entry name" value="PSP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43830">
        <property type="entry name" value="PROTEIN PSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43830:SF3">
        <property type="entry name" value="PROTEIN PSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04468">
        <property type="entry name" value="PSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51411">
        <property type="entry name" value="PSP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PSP1 C-terminal" evidence="3">
        <location>
            <begin position="861"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="379"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="475"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="543"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="887"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="17"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="37"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="382"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="394"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="478"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="543"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="575"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="586"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="614"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ86572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1025" mass="113204" checksum="80D2E73B988F38E0" modified="2017-01-18" version="1">MSSITSQHTPGHPNKKSSISDSSSNTSPNASSFVLANPSSTPNFQGTPLNANGYVKDTQSGTPGTKRTNSSGTLLGSSHNRYVSENAEFATNAFYSHDNMFNKMNDMFQQMNVHPSQQNVASNEEKVSDEGIPFYNEKFHSKDKQVQSAVHQQMQSSGSGLGSMGTLFQSPSLVASTHSSPSSTDVNMYGLHGLNQQTQNYPLTSNPYLNNNLTGPQLYTAAQQIPQSNRDQNTALRSSSYMNYIYETAPVYSEGRNDALTSNKSLNDFNGNSKYVNNVIDRNNGNKTNDSIYQMQSFLGGAHNFESTNPVNQNHDNAVLESSNHMQSGYLNTPLTRSLSTPPFDSIRPNLRGKFSGNSYMNVFQNDAPHTSGLDAQGMLNHYSNGVPSATGSNNNNNNNNNNNTNFNAKNISNAQYLQHSSNQQRDYAYDVFRGDQAGETPLSTGSMYNQSQNTNMAQTAGSAVMMTVAESNYDPNSSTPSTPKFFTSAMRSSSSSSSGNPFTTSFGASSASNLNNTATNTGGNPSFYNAAYRNSSAWPAQNSNNLSPMTPLSSSSVSSAGNHIGAMPLGTAPHSHHDHHMKLNHNASYYNSHTGSNKESKSNTDGGPGNQYHTPNSHNNSGNNSNNNSNTTSPNISAFAAPVLPPPQASLPGNGLITTVDNKSEASGNLQKLYKEYGLKYFSSELCFKFITDLKERVMQEFVKPLMAPSKPVKNTRLNQFLNYLINCNEQYGVHSHVEPFVSNQGKKLVLVCLKNGKLELLSVSKNLSCSNTFKQHDLVIIDGDRGNDLGLVLDPELSFPMALLINFLKKKIHFDSLITSLDTHFSNDSFIDTLMNNAKLINPKHYDIVELTQLIIPSKQVLRFAIPQEVTSSLYGKFCEELKALKFAQAKLRNLNNRNSSTALNIRILNAEFQFDKKKLTFYYVCHKRNDFRELIKELFKFYKTRIWLCAIPNNLDIETKYYSHTLHEYNLVHKNTTTQGRKGGADVQDNLHSRQQFPAVFPDVELDNFQIGVYIELTKQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI59</accession>
    <name>A0A1E5RI59_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Endoplasmic reticulum-Golgi intermediate compartment protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1195</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Plays a role in transport between endoplasmic reticulum and Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Golgi apparatus membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ERGIC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RI59">
        <property type="interactions" value="378"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI59"/>
    <dbReference type="InParanoid" id="A0A1E5RI59"/>
    <dbReference type="OrthoDB" id="5541786at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045888">
        <property type="entry name" value="Erv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012936">
        <property type="entry name" value="Erv_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039542">
        <property type="entry name" value="Erv_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984:SF81">
        <property type="entry name" value="ER-DERIVED VESICLES PROTEIN ERV41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07970">
        <property type="entry name" value="COPIIcoated_ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13850">
        <property type="entry name" value="ERGIC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="310"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter N-terminal" evidence="7">
        <location>
            <begin position="15"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter C-terminal" evidence="6">
        <location>
            <begin position="163"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="362" mass="41249" checksum="64EDC7C645B10456" modified="2017-01-18" version="1">MSXTKDSGSRTFRVLKQFDAFPKVAEQHQKVATKGNNIKSYLWYAFLLFMLWTEFGSYFGGYIDEYYVVDKKPRTTAQMNLDIFVHAKCQNLXVNFRDKTGDRLLVNELLHFEKMPFFIPHTVKKFDSAIDITTPDMDEIFGKAMPAEFRDVVNTDDLPGSENFDGCHIFGNLPINKVYGALQITAKGHGYRSASKASLEEMDFSHVINELSFGEFFPYIDNPLDNIAKLLGPENRLSKFQYYIDVVPTTYKKLGAILETSQFAVSEVVTPVQVGSNGRPMAEVPGIFFIVNFDALTVVVEDVRLNFFQFLAKLVTILCLVFYIFTWFHKLLDKLLVSLGGKKWSLYYATAEPKGLLDSEKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI65</accession>
    <name>A0A1E5RI65_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1354</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI65"/>
    <dbReference type="InParanoid" id="A0A1E5RI65"/>
    <dbReference type="OrthoDB" id="68483at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="114"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="559"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="666"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="725"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="766"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="818"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="867"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="11"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="569"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="666"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="743"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="771"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="818"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="9">
        <location>
            <position position="776"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="928" mass="104111" checksum="AF5133AD8DA3F80C" modified="2017-01-18" version="1">MKQYPSLLDVQGSSTMVEQRSEQSLLSDKPKQLEAQAEDDYHNSLESQSYFSIIETHRDKFKIIPVSELVTLPQLQKSHISPARSHERMAKERDLTPTTIIQSPSTNSRYCNQYKILKEIGQGQYAKVYKAITPVGGLVAIKQFRKKPLNHQSQYSFKQIQAYMMKQQRGKKSTYSGSSVSSSAGTSMGHSSHLSNFNLSTPLTPTTTQTVDSIIMNMTLDKIRWELYVLGTIQDSAYVVRLVDCIDSVKSKKNWIVFEYSPFGEVQWRSLENQMININSHLHKSKKLTSHVLNPLQFCVKFIHDTAHGLQYLKQQCCIHRDIKPSNILIFPDFTFKISDFGSSIIHPDRLPEMGVEKTTVFTKFQNELNKIVGTPAFISPEICNFSNFRETTGLGDNVSPMKSDDALMSPKSNNTSITHQNLIPILDGYKMDAWSLGITVFCIMYGRLPFEGDNEFETFDKIVKDELVEDDLVLRPGLSNIIIADEVGNKQSENAEMIELLQELCIHRLLVKIPQXRIDITELTESVLPSLVEFYNQSLSGSNMPFITVASHSXSKLKKNRSLKRDFSPTSTPKNINHSTXTKPMSHNINSRKPANGSSFSPTSHFFTNTFQINDVSTKTKKESVESPKKIKGFFFSKWPSSTRKKSSPIKAQYIDTMDSPRLASSKNFSGGSTSNSGANTPSIFSQPMAVPLDNDESVSVSSGSSINSDIDDHLAYPHPEIINLSDLDNDDDEGYALEQTSDQSSSDNNQITRINTGEIIERAKLDNVNDNXNENXNXNXNDNXNENXNENTNENTQRQVVEPPARNAERLLAENATISQKPTASSVPLRQPKSAGLVPSRGTIDFTKYLHSTGSQTDIANQLVEPDHASSSSPLVHKTDTPKNSKEKKKNYRTTFLRHASSLNFETPDELTRYLSFADSSSNKPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RMX6</accession>
    <name>A0A1E5RMX6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g639</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RMX6"/>
    <dbReference type="FunCoup" id="A0A1E5RMX6">
        <property type="interactions" value="279"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RMX6"/>
    <dbReference type="InParanoid" id="A0A1E5RMX6"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000919">
        <property type="entry name" value="NPR1p Protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF113">
        <property type="entry name" value="NITROGEN PERMEASE REACTIVATOR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="655"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="130"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="219"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="428"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="624"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="883"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="136"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="223"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="236"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="268"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="325"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="358"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="431"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="446"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="482"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="517"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="558"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="579"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="905"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="684"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ88248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1004" mass="107513" checksum="EB61C01CECBF711B" modified="2017-01-18" version="1">MSQLAEGTTASQNLPSRSSSTLTQLFQDKTNQSSSATPTQNTMQATDTSRNNSNASALDINKNGFSQSFIANNNAHTAVFGASLYGSTNINNSNNIMSSSPMTLNSNSSAGTLNTHKSFAQKNYPSLSSSIPYSVPNSNPSPGEENNGNINNGNNNFNPGTSASSNNNYRPGSSGGSSSSLMDHYSGLLPNNIQQINSNVIQSPKVANVEPRFVITKQKLQEKQQQSANYHRQSTSGGSSSSSNNNNNNNNGSKTLNHSSSFNKKPMSPSGSSSSSTSMGSHSRSRSGSLSSQLGNLFFNSSSSSASASNSTAKGSMNNTSSKPQSKLTSSLKNSTSSSSSSLSSSMNNGSMQNGGSFSQDSAKNPGNAASYSNSDGTFNPKSVGSTIEEQPGFPHADSSAISASVPKPIRARQSNIYSASRTPVNSLHINTSSMSNKNSNSNSNLTNQMRQNTPDGSVTPGTPGSLKNSHSSGIAKFFKKSSSSSSSSHPNIHGNINSTSKNNNAANSAGSNYGNRYSSSPSVSTFNTNAIAHGNGNGNNNGNGNKSAHTIGRDSRSNSNTSSTASTPTASAAGGPQGSSFTSSTFNGPSSLGNTDILYQASNTSLNNGSQQASVPSTSLLQQAHLQAQNQQQQQQQQWQQKQSASLPFHKRYVKTGDDLGAGAGGTVKVFKRLVDKHLFAVKEFRSKYENERKKDYIKKITGEYCIGTTLKHPNIIATIEIVYDENRIFQVMEYCDYDLFAIVMSNKMSYDEICCCFKQILNGVEYLHSIGLAHRDLKLDNCVVDQHGIVKLIDFGASVVFSYPFSKNLVTASGIVGSDPYLAPEVCIFTKYDPRPVDVWSTAMIFVCMVLKKFPWKIPKLKDSSFKLFCSGRDCDSLSQLVTRTPNPPQYEESETGAKESQQKPQSSSNNAKDPNNLNVGPQRLLHNLPEESQHIIGRMVDLAPACRANIDEIMNDPWVESIKMCFVDENNHVVCPNDSHKHTVVDQSIAHIAGLEKNKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RMZ2</accession>
    <name>A0A1E5RMZ2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Cytochrome b5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g709</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Microsome membrane</location>
            <topology evidence="11">Single-pass membrane protein</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RMZ2"/>
    <dbReference type="FunCoup" id="A0A1E5RMZ2">
        <property type="interactions" value="733"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RMZ2"/>
    <dbReference type="InParanoid" id="A0A1E5RMZ2"/>
    <dbReference type="OrthoDB" id="260519at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000002">
        <property type="entry name" value="Cytochrome b5 type B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050668">
        <property type="entry name" value="Cytochrome_b5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359:SF150">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0492">Microsome</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="110"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="15">
        <location>
            <begin position="4"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ88258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="129" mass="14032" checksum="8D8142957DEF743D" modified="2017-01-18" version="1">MSDAKLYTYTEIAEHNKADKLWLIIEDKVYDCTKFADEHPGGDEVLIDLAGQDATSAFEDIGHSSDAVKYLEKLYIGDVDTASEKPQPSAEELAAQEAAKTSSSEGNGRLVLALFVAAFAVLGYFLHKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN30</accession>
    <name>A0A1E5RN30_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Trafficking protein particle complex subunit 33</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g716</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN30"/>
    <dbReference type="FunCoup" id="A0A1E5RN30">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN30"/>
    <dbReference type="InParanoid" id="A0A1E5RN30"/>
    <dbReference type="OrthoDB" id="941624at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14944">
        <property type="entry name" value="TRAPPC6A_Trs33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037992">
        <property type="entry name" value="TRAPPC6/Trs33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12817:SF0">
        <property type="entry name" value="GEO08327P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12817">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="265" mass="30623" checksum="CFA5B8A48D125D5E" modified="2017-01-18" version="1">METAQNSLNPVEEEHRQYQLYLKNLPTVNYLTYNYMILETVPMCISLELEKQKITNDSDESSSFCIDTKYKSHHMLYRYIKLSPQQQENILKRVKSIGFDLGSKLTDMLVFTKDPNFRFEHNSNGVSQVSSSNVGTFTTNKRVNTDLLSIMKFICRDVWKLLFNKQVDNLKTNHKGTFYLLDKNFDYFKNFAVEPSSKYYSNELDNTTVLIQPYLEICTGLIKGVLSTLNISGVADKDEIICSYEFPKDMPVGTVSFSVFVPINH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNA0</accession>
    <name>A0A1E5RNA0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">UPF0641 membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g672</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNA0"/>
    <dbReference type="FunCoup" id="A0A1E5RNA0">
        <property type="interactions" value="81"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RNA0"/>
    <dbReference type="OrthoDB" id="1898221at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006838">
        <property type="entry name" value="ADTRP_AIG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10989">
        <property type="entry name" value="ANDROGEN-INDUCED PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10989:SF16">
        <property type="entry name" value="AT02829P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04750">
        <property type="entry name" value="Far-17a_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ88361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="222" mass="25303" checksum="3303C0F2FFA46794" modified="2017-01-18" version="1">MTKSKRLATTINAILLVTGTYGLYKCTMVHLPPTLNSAGHKQFLTNISLAISLVYNLLALLTQSSSHLVEKYVFPISVILETVVTLVYWPLKIFFVHLIYQQSANPLQKRGTVLPLHVDMCLHLFPCIGLLFQFLVLKRSQFALSTKTAFIGCLMLGTIYKWYLQILVKPELGAKYPYPFLDIKEPWKSVIMYGVTLVAWGVFMVLQKLKRTYSLKSRAKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNG5</accession>
    <name>A0A1E5RNG5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Flocculation protein FLO1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g995</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNG5"/>
    <dbReference type="InParanoid" id="A0A1E5RNG5"/>
    <dbReference type="OrthoDB" id="4036630at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000128">
        <property type="term" value="P:flocculation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001389">
        <property type="entry name" value="Flocculin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018871">
        <property type="entry name" value="GLEYA_adhesin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011658">
        <property type="entry name" value="PA14_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00624">
        <property type="entry name" value="Flocculin"/>
        <property type="match status" value="25"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10528">
        <property type="entry name" value="GLEYA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00758">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184815" evidence="2">
        <location>
            <begin position="24"/>
            <end position="2152"/>
        </location>
    </feature>
    <feature type="domain" description="PA14" evidence="3">
        <location>
            <begin position="56"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1685"/>
            <end position="2095"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1685"/>
            <end position="1695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1701"/>
            <end position="1717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1718"/>
            <end position="1812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1813"/>
            <end position="2035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2036"/>
            <end position="2052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2060"/>
            <end position="2095"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ88410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="2152" mass="223472" checksum="A04BCD2CD8BA608E" modified="2017-01-18" version="1">MFSRLLLFAFFSIAFFLTKKVHADDYLSACRIPSTYALVDGFDMDFYNYPEYSSVYTESGFYLSGYSRYGKLGQATDVETLTFTNDYDIYNGVYSTSLPTYFHYSGAQMDSGYFTMVATGYFLAPTTGTYEFNLVWADDFSMIQFGGGNAFPCCQQQNGSPEITDPEDVVSVNYSNKNGGVTLTVNLLAGRYYPIRVTYVNISTLGKYAFRFMTPGDTDYRYDCSGYVKSGQTDLDYVCDDEVITTTSLFAGTETTTVETMTFATFTTNGKVISETVEEVIVKIPQSIVTTVTTTVSTTGLTAISTVYNSATSTNSQNGQQTITVAESVLVPFTYATSNSYTPYSGSNTSTFATSTVSTTDSNGLIHVTTFYYVEVPISQASSVIVRPYSGSFTSTYSSSFTTIYGSNGQEEISAVYYVETPIVFESSNTYTPSNVSATTTYSTSTSIWTGEDGTESESLIYYVATPIVTKVLKVFTPGTLGSGTTSEIYSTSINTTIGADGKETDSTIYYIYTPISFVSSATTKASEVSFITTYNTSTITTTGADGLETETYLYYVATPIINQYSTKKFPYSGSEDTTYTTSVITVVGEDGKETETSVYYVQTPIVFSSTDIYSPANVTASTTYKTSVLVTTGADGKETDTTVYYVATPIVDQYSTVNTPYSGSVNKTYSTSVIVTTGADGKETETSVYYVQTPIVFSSTDIYSPANVTASTTYKTSVLVTTGADGKETDTTVYYVATPIVDQYSTVNTPYSGSVNKTYSTSVIVTTGADGKETETSVYYVQTPIVNSNATFSTPYFGSVVTTSTSLLTTVGADGKETVSSFYYVLTPVVNITSTVKSPYSGTTEVTASTSYLTTTGADGKETVATIYIVSTPIMFGLSTFVHSINGTKVSTYSTQVTMSTNSDGQEIEESIFYVDQPVIYTSTVVDMPYSGSVVSTSTETVTSFDADGIEIINRIFYVQRPLVVTASTKISNSDIVSPTTYSTSVATYIGDDGKETETSIYYVAEPVSMISTTVHVPINESVAFTSMSLSTTIGSDGEETIYSVFYENDPIKTITSISNYPYNGSTLLTQGSSVTTSTGTDGVQTVVTIYYVDTPTNIMYQTSNAPYSGSTTYTYATSVITTVDANGDEMVDTIYFVYTPIVFVASTSAIPYTGSVPLTVSTSFITSTGADGKQTENTIYYVETPESHANSSASTSASNVVTSVPLSSTTGITYLTSNAPYSGSTTYTYATSVITTVDANGDEMVDTIYFVYTPIVFVASTSAIPYTGSVPLTVSTSFITSTGADGKQTENTIYYVETPESHANSSASTSASNVVTSVPLSSTTGITYLTSNAPYSGSTTYTYATSVITTVDANGDEMVDTIYFVYTPIVFVASTSAIPYTGSVPLTVSTSFITSTGADGKQTENTIYYVETPESHANSSASTSASNVVTSVPLSSTTGITYLTSNAPYSGSTTYTYATSVITTVDANGDEMVDTIYFVYTPIVFVASTSAIPYTGSVPLTVSTSFITSTGADGKQTENTIYYVETPESHANSSASTSASNVVTSVPLSSTTGITYLTSNAPYSGSTTYTYATSVITTVDANGDEMVDTIYFVYTPIVFVASTSAIPYTGSVPLTVSTSFITSTGADGKQTENAIYYVETPESRANSSASTSTSSSSASALASSSTGFSFSYTHSGRSTPAYYNTTSSPSSAETSDKASSTTPMYSSSLPSTPSSMITVSPFTPGPQSFSPTTQSSAEPQTRTSSEPDTGTLPAESQSSTTNSGFISSAESQNLTSQKEGVSTTESGGSFTKSQNNAAVSTASSDSEAAQSSASNSGSGSSNNGSSNNGSSNNGSGSSNSGSGSSNSGSGSSGSGSSNNGSSNNGSGSSNSGSGSSNSGSGSSNNGSSNSESGSTNSGSGSSNSGSGSSGSGSSNNGSSNNGSGSSNSGSGSSNSGSGSSNNGSGSSNSESGSSNSESGSSNSGSGSSNSESGSSNSESGSSNSGSGSSNSGSGSSGSGSSNSESGSTNNGSGSSNSEPGSSNSGSGSSNSGSFNSDDGQSGFASTSSASGVKVFSTIKPSSTETGNGNPSSNVPDGPSNFSPQVSITQGTPTPDLSISAVAVGSGSHQSSIASVTVSSQYHNNGAHVKVSNSHRWASLTAFIAGIMMFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNI3</accession>
    <name>A0A1E5RNI3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Vacuolar segregation protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g821</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNI3"/>
    <dbReference type="InParanoid" id="A0A1E5RNI3"/>
    <dbReference type="OrthoDB" id="1204at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070772">
        <property type="term" value="C:PAS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010513">
        <property type="term" value="P:positive regulation of phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903778">
        <property type="term" value="P:protein localization to vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000011">
        <property type="term" value="P:vacuole inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024260">
        <property type="entry name" value="Vac7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28258">
        <property type="entry name" value="VACUOLAR SEGREGATION PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28258:SF1">
        <property type="entry name" value="VACUOLAR SEGREGATION PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12751">
        <property type="entry name" value="Vac7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1512"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="403"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="484"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="955"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1293"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1328"/>
            <end position="1359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1676"/>
            <end position="1723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="407"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="429"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="489"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="565"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="585"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="628"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="963"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1005"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1682"/>
            <end position="1692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1693"/>
            <end position="1719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1816" mass="201258" checksum="1D855DA2260F4BF2" modified="2017-01-18" version="1">MEGNKHDQLGSLKSATSLTTSVNSKDDLKKNSVSNMTMETVIVEMPKLKINSKNDKNESIHSNDQSNDNLQQNNSKTLDENKNTQQQQSNGSQVHPYQTRKNSLDSNAPSSLGIIDNRSHSSLSKSFMTSPTRQFFGTHLEDPIYEEDNEDTDDNRDEDGNPHLINNGGYNSYNEYNEDDTLEDPLDLDGKINRNIKYDYDTHNNNDPNIEQNNIHPISSNNTTIDINNDLVNENSVKMNMNENDVKMNMNENENESTTLHSNQLARQNSKLIDVNPLSSFHSHPSTSTLHNKSSSLLISEDGLLKGSYKPNKDGKNELSKMESSKQRKDGNNLSSKDSHQALPNDNHSINSMSSTNSLTNFSAQFNSNSNTNSNFNQLHSSNSNNSNSNLIDNTIMNLSPIKRPSLSLNHGSTRSLQTQNKSTTNTHNNNNDNNNNNNNNNNNQETRLKSVVSNEVVSLNSDLNILRHVGSSATMPALLKKSSDFGDLSTSTQTNLNSGDEEMAQQTGNRALRGTSNRQDSIQSTNSLNITKNRSSINDYPNEVSITQQNEKMAHPKPLSRMPSSIVPTTTSSSSNTDLQSSSDATQPTNNIHESQKNMQSANKTNLIEDGPYTRKLENTNNNMNNKTSMQTLSKKQSTINEKLHSRNSTSLLGRDTSMSSANLEDKSKLNAVSSSSLVIPTDKISTSIVNPTKNARNAKNLRNGSQKNEKDENIDINKILANRTDFFAAKLASATNEKIKSNDDNDEDFVYEDLMIGHNSHDALAHTAMVKNALKGTNMANINGINHGLDKNTSQLNVEATPSEMTTSSTIAAPMVSSDTPANDNTNTNLNTNSASLPVSSQTHRTAQPKLHSTPGNISHQSSVIIDPTKLMVSQQSSQQSLKMKPNVSQELHHQTTLEKLTSKGNQVSSNFPQKLNSKGISSKLSAPVLNATGPNSAASKRLASAAAARHASIGPGPIPQRNSLVNTTGTSGVFAPNSTKQLPGIEKISGKPENQEITSTSPKKKTGHSFKKQASRNSIAEGYQERAQSNLGNFNNHPTELQPEPFGIVNSHESKTEGASSPTFLQGEFRPLGQNHTSNYQPSHPQLRTTASRMFGKHSTASSIKRHVPKDVNLEDYIDELQDSEYDLLTNSHNNDSARFQKNQLFNETLSQFQGGSGANTNFKTRNGVKNLDQKLLQQRGVASVGGNFSPTARDHGFGNVYATDQSAEPINSRHLDHNDDESDTRSEFFYRSNHAKPKKNSKQQLKQFKRLSQSSVGSFVENDLQPPNTNSVFPGRRIFTDNDYEHLGAQSYPNALDDNKPHQSVNADDMDSFYTVGDNFVNDHEYDQHRNNRYSYQSRNDNESDTTDGKELGYVSETCKNGNSKSLRGSQWEAMDQFGSYVTPDTTSHIRNGMSPSHYIADGSDEEDDMHSTSDLNMYFDSLDDEPRAIANHEAPNFTKYGSIGSNLNKNVKGNNILPEDGKSPSKVLLQPRFQTGNYSSAANNMFSPHNYKKRVRKNSIWYKFRNFVYFVFIITSLLVLGFICGFFLASNKDLQSFDLSKIDNILSTNDELLFDIIAVALNAGLFTISISDVELDIFAESKYTSSIPDDDNDGGENLNVPSKAFPSWTTASALDKNMNKYKERAYDDVDSEPWETILLGTVYSLEEPLQFRGTFFKRDFDISGSSIKLKNPGLPDSLPPPPAPAPSLSPSSTTSLPLVSTSSSTTTTRNASSEATKKITSTLSPSKKTLHHFSSMHLLEREAVITPENDVKHELSSNPYEKYEQWKRLNKYEYNLIIRGKAYYRIPFFQNEKYIGVQQEVVVNAGKKRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNK8</accession>
    <name>A0A1E5RNK8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Negative regulator of RAS-cAMP pathway</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g787</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNK8"/>
    <dbReference type="FunCoup" id="A0A1E5RNK8">
        <property type="interactions" value="185"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNK8"/>
    <dbReference type="InParanoid" id="A0A1E5RNK8"/>
    <dbReference type="OrthoDB" id="5054775at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013860">
        <property type="entry name" value="AreA_GATA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08550">
        <property type="entry name" value="GATA_AreA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitrogen regulatory protein areA GATA-like" evidence="2">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="580"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="289"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="625" mass="70694" checksum="37499034AA6A0D22" modified="2017-01-18" version="1">MDENLRKHNANHEQKQKPKYGVNLEKFEQLVNKQNELNNNDHCFFKISPRLFTPNNLQNFRNEELYQSIMGVSKHLEHGQRLRNISWRILNKTASKVNEPRTESLLMNDNPYSVTKNNKNQMHNLDQLTIRKRDGVKSIYKVITPRIKTATSTTASASHHSETSHANKATSEQSGNSGTSLFKKPSSTLFNKTTFPTHLTHPVDHNSTKNTLHNNNVSNIAKSSPLFAQKSKSLFNTPHTSEMNINNNNNNNNNNNNNNNNNDSSQFAQKNGAVKTTNAYDQQKVYYSSDEDIYSTDEDTSSSDIYSDEDNDYEDEYYSHNDNESGMMNLRQPTTAHSDLSKTSTNHSTHAHANQDMNYQKKWGSLMLRKYNNKRSSSDKHESNVASGPHQYGYGNNANYPRDPVTEKKLKNLDSITQEMLLDNQSSVTHSSNDSGIKAKVVVPTIPTGPNHSSNAPAQNNQVLFRRGSFGSMVSEATRKRYVHASNAPPTAQTLLPIAFQTHMFVPNDISLQRQEMEQKKILLFNDEDANDMAIDPDMSKFSNPLGKANVTVHHAANVEVAGESDEEIEEESLFYSEVDRNLLNSKDQPKQQQQPGKKYSTAKHNEHRPCSPHHSLFTGGRTRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNN5</accession>
    <name>A0A1E5RNN5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g764</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNN5"/>
    <dbReference type="FunCoup" id="A0A1E5RNN5">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RNN5"/>
    <dbReference type="OrthoDB" id="4036106at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019433">
        <property type="entry name" value="GPI_ManTrfase_II_coact_Pga1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10333">
        <property type="entry name" value="Pga1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="221" mass="25444" checksum="29EC8B3CD55ACF91" modified="2017-01-18" version="1">MSVLFPVEQLKNDLKTPKISTNQRQTWKIRTEKAAQIMKLSLMLAFLCMHFFQIARANTETIIIEVPSYIREDLIHRETRNNNANSKQDQISLAHSNHKTQRFEVVPNARTLVEVLDYQKSSKYMAKICWSAIHPVSIEAIEYEFDEDMSLLLSFDVVQSGPILNQKIIPINVSVDQLVLGIPVTLFSVIINIVVVLMMSCGIIYKYLWKEVDKSDTKKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP03</accession>
    <name>A0A1E5RP03_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein WHI3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g619</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP03"/>
    <dbReference type="FunCoup" id="A0A1E5RP03">
        <property type="interactions" value="160"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP03"/>
    <dbReference type="InParanoid" id="A0A1E5RP03"/>
    <dbReference type="OrthoDB" id="3972316at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061157">
        <property type="term" value="P:mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008361">
        <property type="term" value="P:regulation of cell size"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12245">
        <property type="entry name" value="RRM_scw1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000089">
        <property type="entry name" value="RNA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10501">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN A/U2 SMALL NUCLEAR RIBONUCLEOPROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="539"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="459"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="635"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="635"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ88612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="706" mass="75127" checksum="226DC53AA27D1A8A" modified="2017-01-18" version="1">MSTTTSSSSSSAAATTAAESASGYGLKPTRSSESTSSAQPSTLNSNNNADSIANQTQKNSFSINHRHSIAPIMQTARASTNDPTQQLNHMLNRLSFSAGPGSSGLSGTSRANITSSNMSSPLVANSNIGLHYMIKIRNIPNDITLREANVMFALSQSALVDIDVIEESGTGEKCIHVLWNSLNVALSNALLLDSKTTLFGPDFPFKSFIQLYDVQSQQQIPFNTAANLASTQNSAHRPSLMRTQTDPMQLQQQQQQQQQQQQQQQQRTLFSFNDPFGSRHNSAVIGSQQSAHLIPGSAGGHGNNTSLQASHEMMIPPSPFEDQSFSYDDVPSSASSASNIWNTSNMNGSVSNNVLLASGNIDDSAASNVSRATTFNMPPAPNSSTISSRAPEWAQRSPSIYQNPAVPANLDHSPSATYKNSIFNNGHGRGPLETNFIGSPLGKKIREDIGTQLPYNTAEGVNTQNLSPTSKNASLATADSQASRETLKTNNNNEINAVANKQNVPNAFAQIEGVTEEDLVLLAKVPAPANPADQNPPCNTLYVGNLPPDATEMELRQLFSQQKGFKRLSFRNKNNGNNHGPMCFVEFEDVILATRALASLYGSQLPRSNGSTHYGNKGGIRLSFSKNPLGVRSSVSAGSGPLTNGHSRRSIAASASGAPTTMSVLSTLSDADHGRMSLSARNSVAYPSAKAALTSSSQEPSNTFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV45</accession>
    <name>A0A1E5RV45_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Translation machinery-associated protein 64</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g384</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RV45">
        <property type="interactions" value="510"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV45"/>
    <dbReference type="InParanoid" id="A0A1E5RV45"/>
    <dbReference type="OrthoDB" id="199771at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11608">
        <property type="entry name" value="eIF2D_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.245.10">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039757">
        <property type="entry name" value="EIF2D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039759">
        <property type="entry name" value="eIF2D_SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041366">
        <property type="entry name" value="Pre-PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036885">
        <property type="entry name" value="SWIB_MDM2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003121">
        <property type="entry name" value="SWIB_MDM2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057429">
        <property type="entry name" value="WH_eIF2D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12217">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12217:SF4">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17832">
        <property type="entry name" value="Pre-PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02201">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25304">
        <property type="entry name" value="WHD_eIF2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47592">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51925">
        <property type="entry name" value="SWIB_MDM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DM2" evidence="3">
        <location>
            <begin position="377"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="SUI1" evidence="2">
        <location>
            <begin position="503"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ90764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="601" mass="67237" checksum="9E50CAF8A6AD87AF" modified="2017-01-18" version="1">MFKKELQCKPQSNLKNSDRKKILQKIGQGYDNAGGELNTFQQGNFENHDGTIHGILYTISHVNGDTKQTLPIWFQVTKSKDLKKWGSLKQTALIPTVYTLWLNPTLLPIVITNDFVLDEKIIGSGANLMKKGCYIPVNDIQKRHQAGQNNSCVXVGIASYKRPSYVKAVGVLETSIDSTANNDENGGVAVTVLHYLGDKLCEQFKTKLDPPHALLQEVKEDDEGNVIEEASPSLETESSKAEIEGKENEIDKEDQLSVNEVDEILTRAMYYTLTNDSTLTLPITSSQFISNHININLPKKITPSMVNVKKSSYKKTSKLLKVWEKEGFMKLKSKGGDFVITNVAAKKDKPELQTFISYQPRGAPKSQQQVETNKQKTGKKLYITSSVLLKPSGPQARVFFPNLKFVEPQDVRAAVEEYVKTHNLIDPKDKSKVLCNDELWLFMGKKLADNGSSRLMPRNQVVTAVTQSSSQLLKYFQIYRPDGSAVLKEPHKLSGKDLKIPTVKITTEMKMRRKIVTKVENLEMYGVDPEELASKLKIKCSASTSIGETHNEVLIQGPHEAIVLSVLQKDYEIQSTSPQYVTCENKLKNKKKSSTTAPSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVC8</accession>
    <name>A0A1E5RVC8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Proline dehydrogenase</fullName>
            <ecNumber evidence="2 5">1.5.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g303</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Converts proline to delta-1-pyrroline-5-carboxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23784"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.5.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the proline oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.5.2" evidence="2 5"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVC8"/>
    <dbReference type="FunCoup" id="A0A1E5RVC8">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVC8"/>
    <dbReference type="InParanoid" id="A0A1E5RVC8"/>
    <dbReference type="OrthoDB" id="5464at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004657">
        <property type="term" value="F:proline dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:L-proline catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.220:FF:000017">
        <property type="entry name" value="Proline dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002872">
        <property type="entry name" value="Proline_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015659">
        <property type="entry name" value="Proline_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13914:SF0">
        <property type="entry name" value="PROLINE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13914">
        <property type="entry name" value="PROLINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01619">
        <property type="entry name" value="Pro_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="FAD-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 5" id="KW-0642">Proline metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proline dehydrogenase" evidence="7">
        <location>
            <begin position="163"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="59"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ90892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="516" mass="57801" checksum="D60819AC01E7B511" modified="2017-01-18" version="1">MHKTTASVQYIVANSMKGGSTKNIGSKLVTPQLQRLNNFQLHKHIHTTSTPNTTASLHNAVLSSSPSSSSSSSSLSSSSSSPSFNNQEDAIQKIMKTPAPSPVHVLKSLSRSELFSLTMIGCCTLNKPILNLVIKLFPFVPLPLIKLFIYKLYCGGDNFEQVLETGKNLQKKGINNMMLSLTIEDAEGTKNIDINHIVKETIESVYKVLQPHLEQQLSKATNATSINEIPPGYIALKPSALVTNPAQVLLNFNNMDPYWQKQRQVLIDNCSKITKVVYDLNQKLLQKYPTRKTPFFVSVIDAEKYQLQKNGVYDLQRILFQKFNPKTSPIVSCVGTWQLYLSDSAKELADEYTRAQQGNYKLGLKIVRGAYIHSEPNRSQVIHTTKQNTDENYNEVMATVIKDLVNKGQDSLYGHLVVASHNYQSQLIASQMLKNVAKENTGKCNIVFGQLLGMADNVTYDLIKNHNAKNIIKYVPWGPPLETKDYLLRRLQENGDAVRADNGWPLLKSIFKSLRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVG9</accession>
    <name>A0A1E5RVG9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Putative copper-transporting ATPase HMA5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g496</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVG9"/>
    <dbReference type="InParanoid" id="A0A1E5RVG9"/>
    <dbReference type="OrthoDB" id="432719at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043682">
        <property type="term" value="F:P-type divalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055070">
        <property type="term" value="P:copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520">
        <property type="entry name" value="ATP7, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520:SF32">
        <property type="entry name" value="COPPER RESISTANCE P-TYPE ATPASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="489"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="534"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="584"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="780"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="821"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1206"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1237"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="11">
        <location>
            <begin position="306"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ90950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1299" mass="143117" checksum="CFC4246373BBBFF5" modified="2017-01-18" version="1">MIFVWFPIFTSKPHPVFVLSTMQGTMETILQNRQVHVSNIHCETCVSAISKTLRDVFHNNIEFDINLALGTVRVVANRVNVFDQDFIASLVFELKKIGFSVDDIDAVPSLDVTSQEQLSKHPSNPFVNKPGKETKWKDWVILPSRAHGTERDETSSGDLSSLRSEDFDAKHNSICEVCHQDTKESDPTVHEFQSVDLENKNGGGSEKKYKLSAAIDGITCAACASTIQGITEPFWFIDDVYIDVVLKTGKFIMNNADPSCVAELKEAIEDAGYDFHIIDSVEEIFPGNRDNQDQSQEKAGKEYTHRRITAMIDGITCAACVSTINGVIEQLSFIEDRSISAVSKVGIFILKDDLQESHTRKVDILKETIEDAGYDFHILEDEPLDLHMKSNHKQRTVELLVEGIHCDQXPRRVVEKLQKYGTCGEFHRSKADSSTGTIKITYLPQVSQDITIRAIINSLEQLQLHVQIQXHETMSEHLQKMARAELRSIVWRLVFTCVFVVPTFIFGVVVMSLLPKDNSFNQVMSRRVAGNTDRTAWVLFGISTPVYFVADWMFHTKALKELFVLWKIKGQWLRKIFKFGSMNLLISLGTSVAYFASILLLIISACSVESMQMTKTYFDSVVFLTFFILIGKLLESVSKSKTSSFLNELSAMKPXTAILHSPTVTETNTSNETVPIQYLECGDRILIKPGESPPLDCILTQGGPTQFDESCLTGESVPQAKTLGDLLYSGTVNVGGHGVVAEVVTLDGDSLLDSIVSSIRDGQLQKRAPIEIIADKVTSFFVPCIVILALVTWIVWILLGYCGALPREYLHTSGATDGGWAIWSMEFAISVFVISCPCALGLSVPLALFVGTGMVAKHGILAKGGGLAFQKGSKVNVVCFDKTGTLTLGGEPKITNYCVSANLGAQGGVEFNQVFKEIYEMEKLSNHPLSKSLISFIGKEFPLSVENADHGNDHTGVEVSEIPGRGLQSENLIIGNEKFMLENHCHVNSIHLANLSTWKSAGKSVILVGRRSARGNDNSHMLVAAFAARDPVRPEAKRVVSLLQQRGIQCWVISGDNETTTRAIAKEVGITNVIAEVLPQGKADKVRWIQENVKLAKSPGNTVFNIPKKIVSAITFKLFQRTNQFGSSSVNSKTQSIVCMVGDGINDAPALSTADVGVAIATKTQASDLALSSCDFALLNPKETLYSLLVLFDLCNKIFHRVYFNFAYAIIYNTIGVPIAAGVLFPLPSHYRLSPTWSSLIMAVSSVSVVLSSLMLKFYKPKDNGADGNPKSKNPHSQGISEINRIETNTMAAIQEHFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVH2</accession>
    <name>A0A1E5RVH2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal lysine N-methyltransferase 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g314</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the class I-like SAM-binding methyltransferase superfamily. RKM5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVH2"/>
    <dbReference type="FunCoup" id="A0A1E5RVH2">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVH2"/>
    <dbReference type="InParanoid" id="A0A1E5RVH2"/>
    <dbReference type="OrthoDB" id="2529286at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF109">
        <property type="entry name" value="RIBOSOMAL LYSINE N-METHYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ90921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="339" mass="39141" checksum="D5E70FE78AA2E3ED" modified="2017-01-18" version="1">MELTLGIESKNEDTIVLEFEPPNSGLSSLRKLQKRTQRKSKKLESPSSTNLIKHSYHLELKQKLCSLNSSRDNNNSTTGYVLWQTTPFFIDWLLYHTDSNTQEQNFNLRAFVFSSNVIELGSGVSCVSTVILCNYLSNSNYYVVTDQKGILSTLKENIITNKHEIIHNAKQLKSVTMNWSKPLKESFASNNEEYSLPEEFFHIEVEPLDWEYVFSQKLPCFVEKALGDTYSRNCTILAMDVIYNDFLIKPFLNTLDTLFKKWESENDSSRLTKNDTQCEIKCEKKGIKAIMGLQLRAQDVVQDFLLEAVLEYEFNVQVLHSDYLNSSRFCFYVISPSTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVI4</accession>
    <name>A0A1E5RVI4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Maintenance of telomere capping protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g366</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the WD repeat WDR59 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVI4">
        <property type="interactions" value="387"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVI4"/>
    <dbReference type="InParanoid" id="A0A1E5RVI4"/>
    <dbReference type="OrthoDB" id="311712at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035859">
        <property type="term" value="C:Seh1-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16488">
        <property type="entry name" value="mRING-H2-C3H3C2_Mio-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049567">
        <property type="entry name" value="WDR59-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049566">
        <property type="entry name" value="WDR59_RTC1-like_RING_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46170">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46170:SF1">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17120">
        <property type="entry name" value="zf-RING_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0926">Vacuole</keyword>
    <keyword evidence="3 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="111"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="198"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="RWD" evidence="8">
        <location>
            <begin position="457"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="692"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="698"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="740"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ90952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1170" mass="132478" checksum="3F30CC792DCFCCBA" modified="2017-01-18" version="1">MKNGFVGEDPFTSSTFGKSLSLRVDGGVNAVSINPSGRDVVLASKQGLLIVDLDDPFQPPRLLRHLTSWQVADVQWSPHKSKPYWVISTSNQKAMVWNLARSSTDAIEHVLHGHSRAITDINFHPDHPEILATCSIDTTVLAWDMRSPQAPYYKTADWRAGASQVKWSTNDSNLIASSHGNYVSMWDVRKGTTPLFQMHGHTSSVNNISFDPSSKNRIMTSSNDGTVQYWDYTAACATPTKTVITDFPVGRGRFLPFGDGSCVIPMIEGGNNIYLMNHEFFETDDKEVKLQPIYVFKGHKDRVTDFLWRSRHSENSLTXDREFQLVSWSNDSDLRLWPIPDIVYEKVNFSRKQKLPSPLPDFSYHSYREEPIQYRDKEEFTKIIGTDASYRYPKEHFVSTVPGGRNTNEADRIDHLAWLSGVRVNNDDDNGGQVANTLLYSDNGYNLASAEYRNLGEEFSGVGHKFSKLNFEKISVSTGELVITLNRPSNKEQLVFLRVEADFPQGYPRKGRYPKFHIEENREFTKETRTELETTLSDIAIKYTNKEKYCLEPCLRFLLGENVDLTILDEEDEGLVDFDIMKKLGLDDDFGLSENTRNMSDSDGSIDSGTHFEQDFETTAELGGKNNKNHFETLGGQDFDSTPVPKGCGAVWTAQGQLLCFFASSSKSEKQQKVKEKLFKFSANGVRSFGKLKSSNKNSDKRITSTSDKTKPRPKTYLETLQQPDENDEKTLDSSSGSDFDTDDSLEDESSEEDDFDDILNNDITFRKKIPIISMRANSVSEAYSEGEKTAESSKVGKNIVCVRDFSHVIPDSLNLALNYRLMGEEPEVLCKYNASVAESFGFSDISTCWKIISDMLISQSKDIFNHQDWCFDQYGGQVFIKQIIAYFELTKNIQMLAMLACLLVTPGISEELQTKVNSDMVESIISYENDSRNFSSSRSTPMGYASSIHLQNAHFDGSSVRSENYFNHKEPMLRSQSTXRHTSMARSSLEEPXANYFNQFDDVHNILPDVKVEVLATEISDLSNGSSASLLDPRDEEKLRSFRLQYAELLYLWGLPVHRVKILKFNINHNTELGFINSLKTSETSDIYKGVDSRFVSPFQSVGQHAAYTYAEIHNCCFCSTPVKRRLFVCNLCQHIMHARCSNQWWKEESECPTGCGCRCVSFEETGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVK4</accession>
    <name>A0A1E5RVK4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RNA polymerase I-specific transcription initiation factor RRN6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g540</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVK4">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVK4"/>
    <dbReference type="InParanoid" id="A0A1E5RVK4"/>
    <dbReference type="OrthoDB" id="4090074at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070860">
        <property type="term" value="C:RNA polymerase I core factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001179">
        <property type="term" value="F:RNA polymerase I general transcription initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001163">
        <property type="term" value="F:RNA polymerase I transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019350">
        <property type="entry name" value="RNA_pol_I-sp_TIF_RRN6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048535">
        <property type="entry name" value="RRN6_beta-prop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28221">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28221:SF2">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10214">
        <property type="entry name" value="Rrn6_beta-prop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RRN6 beta-propeller" evidence="2">
        <location>
            <begin position="281"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="761"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="780"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="809"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="823"/>
            <end position="835"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ90904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="835" mass="94674" checksum="804BF4673773880F" modified="2017-01-18" version="1">MTSQSSQRHLRMEGSLPPNYTTKYPYFFSKGVQLDVGSNTTNAYSVDNKWESVKIGDRCFEPRVKFGQEKRVILNLAGPSDNVSEDSSISLPTHLVSEHELETFSXFTPWESTLPQAQSTQALIDXRKTGLVHTSFYSSFPRSRSVTPSEFIPNLKXPTLKFNQQDKGGVPHIKAQIQTVFALGHMQTATDLRANKDSKPVLCFSSGPDNSVFEIYYQSRTLLKVECESKILKIVPPVFSEVALVYSDHFGLVLENGNVMVLKILEVYEEKALFVRKVFQKSQFQSNNLVDVVFSPFDKNQAAFMDCVGNWCLMHLKQTGSTLKLVLLRNVCGSMFDPEELDSIWRRVIWSKEHDKLLLFDETKIVSASLNGNTDLLQLSENKTWSRIHDVAKVGNYLVCLTTMEIQILHMNGSGLLSKAFSWKHYFHHEQSLELSAVSSGNEYMYVGIHLRNTSVLFFYQFLVSRNEPIRVTQSPVYVDFGNPLECFEVVCGDLGTYFADERAGTSLPNVDFGVLYKLQGENEICYMDVAFDKGHNYFLPINHERTHFNLCIPRASFNFLRVLDSKIKGDGSTSAPHNTIDEVEKYENYGLVLESHVSEASMLPFVKSLSRFEIPHFDVNDSEEFLSFIEQIGKHFNCKYPITEIKSLLSAQLFTSKASEFEEVFNSISSAMSLRQRXDLSVFLKTIVLDSLCIFNLTSLKAKNKXDTKNLNFEYQSLLAEWSLNEAVDPIHKAHLELNSLNHTNTTLRLQTEXQLPEIRLSQSSQSSNQKRKQERVQESQVSGFGSQNFNQASLPSSMPPGFSLVGSQQALASQQSSRSQPTKKRKKRAKGFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVQ2</accession>
    <name>A0A1E5RVQ2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g566</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVQ2"/>
    <dbReference type="InParanoid" id="A0A1E5RVQ2"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="413"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="519"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="569"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="413"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="665" mass="72780" checksum="2F4B80D1D86A3599" modified="2017-01-18" version="1">MSIIANQHEMQSNKSQNAIPQTNSSQIPNTEWNCRNTLRNAFNTIKQHFIENRASFFLNKDGLPKEDVLENANAAENFASRPMAIEANSWSPQLTQSSSRITQSNLRIAQLSSSFTPITSGIASQGAFKFAFKSAFRWNYVSLGLANAALGSEYASAGATKGIRNFTETRSQGGQFSSSSSPILQMIEKERAAKKSKRARMRGAKAALKRKLHKSKAALKCKLHKSKSELKCKLHKSKSELKFKFADRGLFKACGKSAQAYEFACKRVQPAESLRAGDSLESRGSNTNDANPNSETALPVSSTSALDYGAVPNDGFSEQETLGNKKAVKRSKRARIRDAKAAFKCNVQHSKARFKCKLSKCSFLKVNKDHYQANEYSNEKSRPDELLREIDTLTSNGVDIDSTEEAAGLAMLDNSTSDASPDTPSTNILDISAQGSPSNTQETQASTLATEDKTSEGELVQQIIKKVRFILPERNYSDDKKTLAHAYNFAPLGGSNTTSNSNFAFCNIGYTVEQKLEQDAADDDAADDEQDAADDDEQNVADDDEQNAADDDTADDDTADDEEHEICEAIKAGSYSKSRENDYSGNKKTLAHTSNITSLGGFNTTSIPNFAPCSTGHTAEQEGEVLEESVKNPNFQPFHNPIPAIEKYFRGWGASLNSTPPLKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVY4</accession>
    <name>A0A1E5RVY4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Protein ERP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g544</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVY4">
        <property type="interactions" value="425"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVY4"/>
    <dbReference type="InParanoid" id="A0A1E5RVY4"/>
    <dbReference type="OrthoDB" id="3427at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184894" evidence="9">
        <location>
            <begin position="26"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="10">
        <location>
            <begin position="35"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ90908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="223" mass="25293" checksum="A7C745476FDB8917" modified="2017-01-18" version="1">MKLASNLASAAFLLIFMCFFQNTNAFYVFMTGGERKCFSKELSKDTLLNGKYILQAYDAAIDGYRDASXNELGIMLDVEEVFDDNHRVVHQKGAASGEFTFVALESGEHKICFQAQSSGWIAKVKTKLDVSFEVGSDIKQLDSKLKGTQKTLQNKVNILHAKVSEIKREQALVREREATFRDISESVNSNVMFWFVIQIIVLGLTCFWQLKHLKGFFVKQKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZE6</accession>
    <name>A0A1E5RZE6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ATP-dependent RNA helicase DOB1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g27</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZE6">
        <property type="interactions" value="1228"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZE6"/>
    <dbReference type="InParanoid" id="A0A1E5RZE6"/>
    <dbReference type="OrthoDB" id="64767at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18024">
        <property type="entry name" value="DEXHc_Mtr4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13154">
        <property type="entry name" value="KOW_Mtr4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000083">
        <property type="entry name" value="ATP-dependent RNA helicase DOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000141">
        <property type="entry name" value="ATP-dependent RNA helicase DOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.300:FF:000001">
        <property type="entry name" value="Mtr4 exosome RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.30:FF:000002">
        <property type="entry name" value="superkiller viralicidic activity 2-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025696">
        <property type="entry name" value="Beta-barrel_MTR4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048392">
        <property type="entry name" value="MTR4-like_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016438">
        <property type="entry name" value="SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012961">
        <property type="entry name" value="Ski2/MTR4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF7">
        <property type="entry name" value="EXOSOME RNA HELICASE MTR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08148">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21408">
        <property type="entry name" value="MTR4-like_stalk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13234">
        <property type="entry name" value="MTR4_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005198">
        <property type="entry name" value="Antiviral_helicase_SKI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01142">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="166"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="398"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="367"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="68"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="376"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ92321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1078" mass="122338" checksum="C3E74FB5A00E3C12" modified="2017-01-18" version="1">MSSGDLFNVFGGNGQDLSIDDIDIEDDVVETVVDEDPNETQKADKKRAPSEDESEDEQNAVQSVKKPRNFEKKLIDKKESKKDAQNVVAADSFEQEASREVEASTGLSNNATMEVGEDGKVKLSHQVRHQVALPPNYDYVPIGQHKRQNEARTYPFTLDPFQDTSISCIDRNESVLVSAHTSAGKTVVAEYAIAQSLREKQRVIYTSPIKALSNQKYRELLAEFGDVGLMTGDITINPDAGCLVMTTEILRSMLYRGSEVMREVAWVIFDEVHYMRDKERGVVWEETIILLPDKVHYVFLSATIPNAMEFAEWICMIHSQPCHIVYTDFRPTPLQHYLFPAHGDGIHLVVDEKGTFREENFQKAMATISSGDDPASSKGSKNGKSYKGGNSKGSSKSDIYKIVKMIWMKKYNPVIVFSFSKRDCEDLALKMSKLDFNTEEERDALTKIFKNAIELLPEQDRELPQIKHILPLLRRGIGIHHSGLLPILKEVIEILFQEGFLKVLFATETFSIGLNMPAKTVVFTGVRKWDGTQMRWVSGGEYIQMSGRAGRRGLDDRGVVIMMIDEKMEPQVAKGMVKGQADRLDSAFHLGYNMILNLMRVEGISPEFMLEHSFFQFQNSGAIPLMEKKLKALEEQNSQIVVEDEYNVKDYYDIKQTLDSYHNDVRLIMTHPAHILSFLQAGRLLKIKVDGKYDYGWACVIDFVKRTNKRDMSVTYTDHESYVVNVLVNTMYVDSPINLIKPYNPQYPEGIRPAEPGEKSMSTVIPVTLASIQEVGKIRVYLPNEVRSPKQKETVGKSISEVIRRVQGXVPTLDPVSGMKINDDDFMKLLKKIEVLTNKANTCPVAKSVKLPELYENYSKKVHMNQQIKQLQNKITEKQSIIQLDDLRKRKRVLRRLGFCTSDDIIELKGRVACEISSGDELLLTELIFNGNFSDLKPEQAAALLSCFAFQERCKEAPRLKPELAEPLKTMRELASKIAKVMKESKIEIVEKDYVESFRHELMEVVYEWCKGAPFAQICKLTDVYEGSLIRMFKRLEELIKQLVEVAGTIGNNALKEKMEAALNMMHRDIVSAGSLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S0A8</accession>
    <name>A0A1E5S0A8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">MICOS complex subunit MIC12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">Altered inheritance of mitochondria protein 5, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10 12">Found in mitochondrial proteome protein 51</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g59</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Mitochondrion inner membrane</location>
            <topology evidence="2 12">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the MICOS complex subunit Mic12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5S0A8"/>
    <dbReference type="FunCoup" id="A0A1E5S0A8">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5S0A8"/>
    <dbReference type="OrthoDB" id="4037694at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044284">
        <property type="term" value="C:mitochondrial crista junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031463">
        <property type="entry name" value="Mic12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17050">
        <property type="entry name" value="AIM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ92445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="99" mass="11319" checksum="EEAEA4D6DB70E21F" modified="2017-01-18" version="1">MSKLVKLTSISILSSATLGYLYYNNQYYFKKSEYNQIDNKVVSIIDRKGPAGLQDGTSQTELEQFAPGHYLTRNSKAEVMKDIWNDSVRTGINWIYSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0B6</accession>
    <name>A0A1E5R0B6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PA14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g4574</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R0B6"/>
    <dbReference type="OrthoDB" id="4070743at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000128">
        <property type="term" value="P:flocculation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001389">
        <property type="entry name" value="Flocculin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018871">
        <property type="entry name" value="GLEYA_adhesin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00624">
        <property type="entry name" value="Flocculin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10528">
        <property type="entry name" value="GLEYA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184541" description="PA14 domain-containing protein" evidence="2">
        <location>
            <begin position="27"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="domain" description="PA14" evidence="3">
        <location>
            <begin position="41"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="684"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="738"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="747"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="4">
        <location>
            <position position="433"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="4">
        <location>
            <position position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="940" mass="100470" checksum="DBB077D2646B6739" modified="2017-01-18" version="1">MLTSYLKLVAAVFFLSGSQLLHVVFASNHYVSSCNILANQLENQGFQIDFYEYPEASNEYRASDFYTTGYKNYNHIGQCKNVNQPSLFLSDEGDLPEEYNAPASMERVSSYTMVATGYFLAPFYGNYQFTVSDLSGAFHXSIGAENTFPCCQQQNGXVESELDPVNAFSVDSSSGVTEQTLTVYLLRYQYYPIRFVLVDALGEGQFNFEYMGPLDTDPSDDFTSHVFSSNEDSESNCANPIVSQFVEWGSTTASSSADLAYATVTNNDDVLTVGFFKEYVFYTPEPTTVATTTTSNVVGLNEQSTTTSVITSTDPANLQIIYSTVIVINVPLVESTTTVYDPWAASTTRVMTDSTYWSYANGTSYFLHKYFAFSPFHYAMSTIQTAALVSATTTYSTGFYTQTGTDSSATLFTVYYVETPIISTTSCTTTVSDVADTTTYSTATFNTTDAKGGETIATVYYVATPYGHTATTANSPYTGSATVTYSTSLSTMVGDDGKETEVSVFYVETPIDWETSTVYAPETVLTATTTLTSTAVTTGSXGKQTNTVVYYVATPIDMATXTVYLPYNGSAASHDVSFVTTTGLDGKETVSTMFLEYNPVATATSTVNVPYSGSTACTYSTFYFTTTGENHIETDGTIYYVSTPFTSSSMALSVSGSSSSSAASGLSTKTSLXVIGTSTTSLVSSTADSSVKPSSFGLPSSIPSANNTSSGKDSPSXASISYSRPFYYSGNSTATMPQETTVKHKSSAVLSSPPASVASESTTTTTTRNVVFLTSEVLCSTETPSVATLSLFNSLEQNSFSSLPKSESVVSEERSAKSVXIEKTAIPVQASSDIEVISTLIVTTVSSATTTSQTSALQDSQLLLLTTTNAGQTESYSFLSSWAANTAASSTHTTXSVSAQIASAQTVATQYNNQGSVETVNCKSIFSILLATAFAFFVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0G3</accession>
    <name>A0A1E5R0G3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g4446</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0G3">
        <property type="interactions" value="174"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R0G3"/>
    <dbReference type="OrthoDB" id="4953at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000015">
        <property type="entry name" value="Putative zinc metalloprotease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000031">
        <property type="entry name" value="Putative zinc metalloprotease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43016:SF16">
        <property type="entry name" value="METALLOPROTEASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G07610)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43016">
        <property type="entry name" value="PRESEQUENCE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="2">
        <location>
            <begin position="25"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="3">
        <location>
            <begin position="165"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="984"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="988"/>
            <end position="1004"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1004" mass="113346" checksum="1F503102CEAAC35A" modified="2017-01-18" version="1">MQLVHINSTASPLVEGYFAVPTEITNDSGCPHTLEHLVFMGSKKYPFKSTLDIAGNICMSTTNAWTATDQTVYTLSTAGWTGFKKLLPVYLDHLINPVLTDEACTTEVYHIDPETFQDKGVVYSEMEGIESQSWFITSLEMQRLLFDKDSGYKSETGGLTANLRNLTNDEIRQFHKDMYSPDNMCLIITGNVPEDELLQIVEEFDGSLNAVTEKRVRPFVETATAKTLVPHFHQDIVESTVRFPEMDETQGEMRFSWIASLYTEGVSDLALACLLEYLTKGPLATFQKELVEIEDPLANFSEYDTDDYYRSILNLNFYGVPTEKLQEAKNAVLNILSTHKVDIERMKQVVDSYKLDYIIKCENKGADLLSAACITDFLYEKDEYGATLKQSVSNLKDFDTIYNYSTEMWQSVLDKWLVQNKPTIVIAEPSAEVYEQLNKXKEERLAKRKTEYGEEGSKKLKAVLDNANAKNNTPIPENVLEEFVLDDPASSVKFIETTSISSVAAHNDESNEVVKQVLANKPADLPMFIHVEHFPTQFVEIHACLNSKFIKDVELLPYYLLFCDLFTMPMKLPDGSIMPYEDVVACIKKETLDSDVLIGIAGNFQDFVNARLRAKAENYEHSVGWIKHVLQDMVFDEKRVTVLLENFLSSIVETKRSGSSMLYSLVKEHLFEARSLSKSTDVLFVEELLKEVLEQIKAGDYKTKVFPKIETFRSDLRAHLGESHIVVLGNVEKLGVTEIYAPWVKHFGNGSVNKIPEIPKACDNLSVLGKTTGHKKAFIITTPASESSFMSIHTPLSINYDHEDFPALLLAAEFLQGAEGPFWKGIRGLGLAYGAYTSVNDQEKQLSFEIYRGADIIKCYETGKQIIADFASGKSKIDSLVLKGALSLRINRIVQQEDSYVRSALRKYVSVFGKKLPLNYNEEIFKKMAQVDEAELIAVIGKYFVPLFEVTTATPFIACHPSNCELITEFFEKMGYVVSVKELHQDENSDSDEETDYSDSEEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0M6</accession>
    <name>A0A1E5R0M6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein FMP27, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4626</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0M6">
        <property type="interactions" value="252"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0M6"/>
    <dbReference type="InParanoid" id="A0A1E5R0M6"/>
    <dbReference type="OrthoDB" id="1562405at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019441">
        <property type="entry name" value="FMP27/BLTP2/Hobbit_GFWDK_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019415">
        <property type="entry name" value="FMP27_SW_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019449">
        <property type="entry name" value="FMP27_WPPW_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045167">
        <property type="entry name" value="Hobbit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678">
        <property type="entry name" value="ANTIGEN MLAA-22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678:SF6">
        <property type="entry name" value="BRIDGE-LIKE LIPID TRANSFER PROTEIN FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10344">
        <property type="entry name" value="Hobbit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01214">
        <property type="entry name" value="Fmp27_GFWDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01215">
        <property type="entry name" value="Fmp27_SW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01216">
        <property type="entry name" value="Fmp27_WPPW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27 SW motif-containing RBG unit" evidence="5">
        <location>
            <begin position="937"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27/BLTP2/Hobbit GFWDK motif-containing RBG unit" evidence="4">
        <location>
            <begin position="1050"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27 WPPW motif-containing RBG unit" evidence="6">
        <location>
            <begin position="1414"/>
            <end position="1877"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1561"/>
            <end position="1588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2419" mass="277791" checksum="EB5F84ECF8D28B2C" modified="2017-01-18" version="1">MWAVSVITNSLPKYARHYLQDQFQTIVLLLLVYLVWQKLLTTFLLTVACFFSKGKLANTSIRFWFLNVIYKTKVNLTHNSSIFVSKIRLNKKKIDNXNHKTLVLDLVDVTINITEEFLSSQKSQKKKQQSSDSQSEDDKQIKMEVSLEKFMKQKKIIQYIASFFIPLQVSLQKISLVVDGQEYYVDYASAKVIFNAKSKSLSANIFLHEFTDYKGTYLKHFEFNVSGSCNYSEESNMLSIRKWSTSLRLSDFTASIPPRNTSCEKDTGYTSDQAAQPKSRKESLSXKLQRFQPLWKAMSVVDLKFENITISVPQYCTKFHCANMIFSVKSLTHLSRDSKFEMTLSSNSLYVELLKQRVVVIPLMNIFSTSDRSLNLDFNTINCTMGLVDSKITFGHEHMKYFLHHTGSPSKSQDAESSSHLNNGSVFDSLTIPKFNFKLLLPNFSIRYMTDDNSFFIWKNNEISVHLQHSYLTTSNISVKAASRDALVRINQPTLERLTSSNYIKFSDSSLTFKYENSDLPIAAVHKIEVQNTNDNPHVLNFIVKNFHITLNSLKTLTIGKEHFHSLQQLLATSKPKGPAKGKTDTHSSKFENLEIKLQLIDFSCMSCISGFLPVQNDVPKEXYNTAYGVSLYVDNAFVXYKNKLTKADXDNIYVNKVISVNECEAEQSFVSRMQNITFTHSPDKMKKLTIPQFRIDLDICLLWMIFYMKTVFATILAGSEGNAAQKTDKNDTKSNLSTFDVQVNVILIKGGLANDLKLLFTCYNLKFKXSALSFKRFHILTHSVYVPEQADEVYVRLFIIDGFKFNLLSKVNDQVFDIQTKLIQLRAEYHLRFYTLIDRIVSTVKTIKQLKVAFQSLPKFKHVVPVAEDPKHVPRIRLRTRRLVMRVEEDPFEHDLGVIYRVGLLEQKLRLQKTMFMEEELYNNADQNMSEETIAHYKEKLYRNFSRSWIERIKRARELFTRVGAFDLTEKTILGSKVKTLFGHEKTSLFLVAFENLDLMVKEPNFGVENFPDFIWKYGKHVPKDTKYTTLIPMNISWSTDLLKFQLRDYLLPILSFSGLTLDGDVCFAEQDADYKNKRSVWVPFIKQDEELEKTNSIFGSHIIRTLNSVKTFMNVRFNIDHRDPSIITWGKSLQPAYQAVMIWFDYFTKPPIDPSPKIGFWDKFRLLIHGKMVWNWSPRSELHLNIKGSSDPYKITDDGAGLTFAWREGTCLTIHGSSNPKDFLVIQSKGFLLGIRDFTTLSQKDRFRKALMQLKGDVTWKAGLLFEAGDQAMAGEEPRTSYFKSHYSVELINPKYTSKKSKHDSYAGFRSDFIHMSIGVYSDNKEGTRNSAHLAPTSSDHFKKWWHLFGTYTSGPIRQGPLFSTLVQNPKKFGASLFTIKYSLSLAPLEISHIYRHPNIGQKSKALTEFTGMKGKASSFKFDLHHKRLKMLLMDDRLQRKKNIWKLKIDQGEVDCKNADVRLMSAEFDGSQKHQSVKDACAEADDLEWYNFFDFIDLEDFELRSDALSKYFTIPLLFSPRISYFRNIDRKVHDLKYPFGNEDSHDCMYGKNHPEDTQRRICQKRIRELEQELRFKKNEQNILIQSKASTKDAKEKLSSLSKSISLLKDSVAALEEVLQDIHIVDNENTDKVVLERRESGGDLSLVKTDTLGSLRQMKLATSNTNTNTFDNRFMIHNILLKINGKSKNLLTNYFIKLGERKTASFYVSHKSLSIVNEMLNWSGTENAPESVPRFGKMTTPGMLSNTEFIEKFEELIREVADEKFQTVDNILIKLILPQIQVSSTAEKEWAAIIAPKNIEVGLIDVVQDDGLYANAINSVESLRETRYCTLLSDTNFYVLNKESVCKNELFRTEGAGYGVVDELHNWPPWIPLEACYQSELISPYSILKKTDIFLTFTKPNSLFFDKVLHSVYEEDPVLRVGFPELHITSNAIQYNAIYNIAEDVMKYGSTVNKKLENLAKTILANELKSNFKLSFTFIMGLQNKIRDIHYQRSVLKLTDPDXYNELCQEIEAELQSTRFELIMIMSSLKKTFAQMRGLKHSTKLLSIISADDIIWEMYHDNNDSFITFGLGHTLFQRTEDSDGANTNKVSISSLRCFNLQQNPVYVDLLAPYEEDGTKVVPGPLLEILWFLGTPVGGISDLEDLTIRCRPIRFQMDHITLNQIIDFLFTTRDTGSSNXDDNQDISSNSTWNGHLKTPTGLKNKLHDRMAGEPSDSPDSLLRVKTIGSWDFEHMPTVQNVESSVNKQNKLTGKIVETGDGMEEMVNRSSKYINIRSLTIRHMTVCVSYRGSKSVITNVDNLIVKVPTISYKNKIWSTSDFIATLKKDIIKVVLHHTGTIIGNKFKPKKKIESKYESFKQLSKLLKNEVKVPNSEDSDRQIVYDDGEKFMHXQDYKPIDALTDGDSLADGNEIHAYNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0T6</accession>
    <name>A0A1E5R0T6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">U6 snRNA-associated Sm-like protein LSm1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LSM1</name>
        <name evidence="8" type="ORF">AWRI3579_g4426</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the cytoplasmic LSM1-LSM7 complex which is involved in mRNA degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the heptameric LSM1-LSM7 complex that forms a seven-membered ring structure with a donut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0T6"/>
    <dbReference type="FunCoup" id="A0A1E5R0T6">
        <property type="interactions" value="456"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0T6"/>
    <dbReference type="InParanoid" id="A0A1E5R0T6"/>
    <dbReference type="OrthoDB" id="10263346at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01728">
        <property type="entry name" value="LSm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034104">
        <property type="entry name" value="Lsm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044642">
        <property type="entry name" value="PTHR15588"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588">
        <property type="entry name" value="LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588:SF8">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Sm" evidence="7">
        <location>
            <begin position="31"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="167" mass="19247" checksum="241C0277AE3D6931" modified="2017-01-18" version="1">MSHNKSGDTTSGKSLVSNGEADLYLDQYNFTTTAALVGSVDRKIFVLLRDGRNLFGVLRTYDQFANLTLEDTTEIIYVEDEKTGEIVQYGEAEIGTYMIRGENVVYLGELDIDKEDKPLERYEKIPFEQAQQFLESNNKRLLIENQRKKDFYIQKGLTYEYHGNGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R137</accession>
    <name>A0A1E5R137_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">COMPASS component SDC1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4059</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dpy-30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R137"/>
    <dbReference type="STRING" id="56408.A0A1E5R137"/>
    <dbReference type="InParanoid" id="A0A1E5R137"/>
    <dbReference type="OrthoDB" id="417678at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22965">
        <property type="entry name" value="DD_DPY30_SDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.890.10">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007858">
        <property type="entry name" value="Dpy-30_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049629">
        <property type="entry name" value="DPY30_SDC1_DD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05186">
        <property type="entry name" value="Dpy-30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="83"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="94"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="112"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ80625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="139" mass="15735" checksum="8D25CB9D411C5A55" modified="2017-01-18" version="1">MTEDRKVSIAETIGGSSVRKYLNENVTKELLKGVRKVALERPENPLLFLGQYLIERSRELENISKQGFPVEKQKISTAAVKKEIPGNANEHDTTINNSRQNTATTVDIQEQEATKETEVEMKEEATKETDVEMKEEPTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1B0</accession>
    <name>A0A1E5R1B0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g4410</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1B0"/>
    <dbReference type="InParanoid" id="A0A1E5R1B0"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ80353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="87" mass="10260" checksum="C19E3FE64E8B8367" modified="2017-01-18" version="1">MQRNESANHLELQWEHIRASPYHLNYHILGFLKTKFNELQLLNNNGDEKKQKMNMVLKKVSLASSSTQAISAQERWCIAQTKKRKYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1L4</accession>
    <name>A0A1E5R1L4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosome biogenesis protein RLP24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3995</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1L4"/>
    <dbReference type="FunCoup" id="A0A1E5R1L4">
        <property type="interactions" value="1029"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1L4"/>
    <dbReference type="InParanoid" id="A0A1E5R1L4"/>
    <dbReference type="OrthoDB" id="10262490at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902626">
        <property type="term" value="P:assembly of large subunit precursor of preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00472">
        <property type="entry name" value="Ribosomal_L24e_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.170.20:FF:000001">
        <property type="entry name" value="probable ribosome biogenesis protein RLP24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.20">
        <property type="entry name" value="Ribosomal protein L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038630">
        <property type="entry name" value="L24e/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056366">
        <property type="entry name" value="Ribosomal_eL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_eL24-rel_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792:SF8">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN RLP24-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="TRASH" evidence="5">
        <location>
            <begin position="6"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="150"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ80798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="196" mass="23528" checksum="AC047FAA615EBDFD" modified="2017-01-18" version="1">MRIYSCHFCSSPVYPSHGVMFVRNDAKEFRFCRSKCHKNFKMRRNPRKLKWTKAFRKAAGKELAVDSTLTFAQRRNIPVRYNRELVATTLKAMSRIEEIRQKRERAFYKNRMKGNKETDFLRDLELVNNNPELLKMREVELRKQAEKEARKLEMENEIDMESETEDMEVESEEEEQVEEPVKIALKNKKRTKKIAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1L6</accession>
    <name>A0A1E5R1L6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3954</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1L6"/>
    <dbReference type="STRING" id="56408.A0A1E5R1L6"/>
    <dbReference type="InParanoid" id="A0A1E5R1L6"/>
    <dbReference type="OrthoDB" id="10069473at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12460:SF0">
        <property type="entry name" value="CID DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12460">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CID" evidence="2">
        <location>
            <begin position="27"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="304"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="414" mass="45729" checksum="CFD605C5D164EB5A" modified="2017-01-18" version="1">MNQTSLPTTYERRLKKDFCTRTILREKQMVNSTEVNLKLKKLNETQDSIVATSKWIFANSVKEPNTFDVILFTISSYLNSSDPQYQVSNKKKLTLVYLLNDLSHLIKFKHADKAEFVQTLDEQIPNLINEAMNAVPGDLQQKIVRVVKIWKQRNVYPEKVLSQINTSVQSSSAGDSKVSSTGADRIVKELKVINDIYTKYKNTGSTKLDLKKEIIKSLDVLIAKEKNEYLDMKRRETNVKNRSTIQSVPVQQPIISATDPSSVAEEIPTYAASSSSSSDSDSDAETQEKQTEQDTAVVEKHGDANGNASRDANGNANGNANGNANGDANAFSEHGNEAVGLPVDNNAHLDTSTETNEQLEEYDPGEQQRAQKKPRLDASPPANGATVSAAANQKPVLLTSNIQDLLNKLSQSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1P9</accession>
    <name>A0A1E5R1P9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Chromatin structure-remodeling complex subunit SFH1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g4087</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1P9"/>
    <dbReference type="FunCoup" id="A0A1E5R1P9">
        <property type="interactions" value="230"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1P9"/>
    <dbReference type="InParanoid" id="A0A1E5R1P9"/>
    <dbReference type="OrthoDB" id="10258327at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006939">
        <property type="entry name" value="SNF5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04855">
        <property type="entry name" value="SNF5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ80821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="384" mass="44135" checksum="8CD9B68BD6655081" modified="2017-01-18" version="1">MSLPPQAFITNFHNRVKNKDEPVTLYGQIQSTKSSRRTKVVNYSEDNNQDFDYDQDEEEEMMFDAENAESGENGAANAAAVPTTKPFAPFPTFEEHADPTNFLKFSKIKETYANGKLTRPYSEMLDLELKFDVDENEILEANKKFKLKSESLPYKENFDTPALLPIKLIFEEPNLDCKIDDYVIWNLNDQGLTPEDFASIYLMDLQIDRSSTLYNRIVSTIYDAIEKQSSYVRAVLPEIHLVIKFSCNLGNTYYEDTFQWNIGESSNTEYQKGVPPSDISPETFAEIVVADLGLNREFLGKIAFSLHETLLRLKKKVIEGDSDEIQLFNNGAPVGLPAGVRLDIEDLGSSWAPKVEVLSEFEIERRKEERQRTNRRMHRRKYVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1R2</accession>
    <name>A0A1E5R1R2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Xylulose kinase</fullName>
            <ecNumber evidence="6">2.7.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3865</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Highly specific D-xylulose kinase which participates in the catabolism of xylose. Xylose is a major component of hemicelluloses such as xylan. Most fungi utilize D-xylose via three enzymatic reactions, xylose reductase (XR), xylitol dehydrogenase (XDH), and xylulokinase, to form xylulose 5-phosphate, which enters pentose phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>D-xylulose + ATP = D-xylulose 5-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10964"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17140"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.17"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the FGGY kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.17" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1R2">
        <property type="interactions" value="489"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1R2"/>
    <dbReference type="InParanoid" id="A0A1E5R1R2"/>
    <dbReference type="OrthoDB" id="1728974at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004856">
        <property type="term" value="F:D-xylulokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042732">
        <property type="term" value="P:D-xylose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005997">
        <property type="term" value="P:xylulose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07776">
        <property type="entry name" value="ASKHA_NBD_FGGY_SpXK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042024">
        <property type="entry name" value="D-XK_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196:SF57">
        <property type="entry name" value="XYLULOSE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 6" id="KW-0859">Xylose metabolism</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="8">
        <location>
            <begin position="148"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="9">
        <location>
            <begin position="318"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="565"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="569"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="641" mass="72584" checksum="A9DFDADE8E37A288" modified="2017-01-18" version="1">MTKGXEIKIDKNGKYFMGFDLSTQQLKIVIVNEQMQLVVEESVGFDKELPQYKTKNGVYIHDEDGTINAPVAMWLEAVDLIFTKITNHENEAVKGILPQIYGMSGSCQQHGSVYWNCSADLQLRDMTDKKNLKENLASSFSHDLSPNWQDHSTQDEIDAFNNLYGIEKLSEITGNRAHYRFTGPQIMKIAKKYPDIYKKTSKIQLVSNFLCSVLVGKLTPMEQSDACGANMFDIPKRQWNRDYMGLIDNSGEPPSIDVLTKLNGEPWPMCGESLPNGLIHPYFVNKYGFNPQCEVFPFTGDNLATILSLPLNKNDILVSMGTSTTVLVVTDQYHPSPNYHMFLHPTMKNHYMGMICYCNGSLPREQLKDKLDPPTWDHFNQVLDAQSESDSGNNNDNEIGVFFPLDEIVPGVRKQTKRYNFDIKTGNLKDPIKNDGTDHVKKDIRTIVESQALSCRVRCAPLLSNDEQGNKDTSSQGDSTTTEKVKFDYDEFPLDNYAKIKPNRIFFVGGASQNDSLIKKFAQVLGSNHDEIYRLKTTNSCALGGSYKALWSYKHQRSQASDFVSGIADSEKQQHKPETTASKAPSSTSVKQDFFEFLNENFDWNQNINRVPIDKEAYDKSFKAYTAKIKPLSQLEQLLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1S2</accession>
    <name>A0A1E5R1S2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="14">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g4236</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="14"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1S2">
        <property type="interactions" value="981"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1S2"/>
    <dbReference type="InParanoid" id="A0A1E5R1S2"/>
    <dbReference type="OrthoDB" id="361536at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02658">
        <property type="entry name" value="Peptidase_C19B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14298">
        <property type="entry name" value="UBA2_scUBP14_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000086">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033864">
        <property type="entry name" value="UBA2_scUBP14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016652">
        <property type="entry name" value="Ubiquitinyl_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041432">
        <property type="entry name" value="UBP13_Znf-UBP_var"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF664">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17807">
        <property type="entry name" value="zf-UBP_var"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016308">
        <property type="entry name" value="UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 14" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 14" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7 14" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="17">
        <location>
            <begin position="164"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="16">
        <location>
            <begin position="323"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="15">
        <location>
            <begin position="576"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="15">
        <location>
            <begin position="640"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="332"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="727"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="18">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR016308-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR016308-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ80846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="774" mass="88268" checksum="F42A2097F8A424A8" modified="2017-01-18" version="1">MEDQVLFESLPQTIYKDDCGYCFRSMYNEHHDGNEGLNLCLSCFQSFCDGHLSKHFTAAGNEHKRILYIKKTKKEELNXXSKEGDLGNETAIKKIKLEVKNEPKEDEIYNTFWAVGYLNNDGTKHYTVDNASCSDHTMQQVHQILNQKNQDFQEMSNAWQLELRSCGHIKELQEKIISNKAVLXLDPDLKCSSCELTDNLWLCLXCGNLGCGRQQVGIEGHSHAVKHHASTQHXLAIKLGSLSKDSRDIYCYTCDDEVKFEEDFVAANYLSNYLQNLKLDSKEQTEKSLTELNVEQNMNWDFQMKDSQGKELEHLLPNKELGCGLINLGNSCYMNSIIQSLVNFWLARDXSELEKYLVFPEDVVYVNTNFKCQVIKLLNALCKESFNYPNGVKPTLFKKLVGGSHEEFKSGRQQDAMEYFTFIINTFDKKFFNSSTNNNPNDIFKFVLEDKLKCNNCQKVKIMEQASENLQIMLDENLDEQNFGDQINAYFQGDQNIDFKCPNCGDALQMSKVPYMKTFPKVLVLNPTRLKLVNWQPVKTSSSLDFPELLDISSYKSKALQKSETLFAENNNSGFEPNQTALEQLMETGFSXNACERALFHTGNSSNPDSAMEWIFQHMEDSDLNARFEPPVQNSKXNNSXDTESLSSMISMGLPEKLCKKALILNSGNVNVAVEWVFNNMDDDGELPAAEQTVSTPENLYGDENGSGKYSLQAIVCHKGNSAHSGHYVVFIKKRVDDKDQWVLYNDEKMVIATDVNNLEDMKKNSYIFFYSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1X9</accession>
    <name>A0A1E5R1X9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protoplast secreted protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4227</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WrbA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1X9"/>
    <dbReference type="FunCoup" id="A0A1E5R1X9">
        <property type="interactions" value="420"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1X9"/>
    <dbReference type="InParanoid" id="A0A1E5R1X9"/>
    <dbReference type="OrthoDB" id="504689at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032126">
        <property type="term" value="C:eisosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003955">
        <property type="term" value="F:NAD(P)H dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160020">
        <property type="term" value="P:positive regulation of ferroptosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000001">
        <property type="entry name" value="NAD(P)H dehydrogenase (Quinone) FQR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010089">
        <property type="entry name" value="Flavoprotein_WrbA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01755">
        <property type="entry name" value="flav_wrbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002999">
        <property type="entry name" value="PRK03767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546">
        <property type="entry name" value="FLAVODOXIN-RELATED PROTEIN WRBA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546:SF23">
        <property type="entry name" value="FLAVOPROTEIN-LIKE PROTEIN YCP4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="2">
        <location>
            <begin position="4"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="201" mass="21359" checksum="3DF6E63D1D30ED8D" modified="2017-01-18" version="1">MTKVAIIIYSMYGHIAKLAESEKAGIEKAGGKADIYQVPETLSDEILAKMHAPPKPAYPIATIETLATYDSFLFGIPTRYGNMPAQMKNYFDATGGLWAQGALNGKFFGVFVSTGTGGGNESTVMNSLSVFVHHGMIFVPLGYAKVFAQMTSFDHGVKGGSAWGAGTIAGADGSRSPLDIELEMASIQGSSFYEVVSKYFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R289</accession>
    <name>A0A1E5R289_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Origin recognition complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g4239</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the origin recognition complex (ORC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the ORC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R289">
        <property type="interactions" value="1038"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R289"/>
    <dbReference type="InParanoid" id="A0A1E5R289"/>
    <dbReference type="OrthoDB" id="346673at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007220">
        <property type="entry name" value="ORC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056772">
        <property type="entry name" value="RecA-like_ORC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056773">
        <property type="entry name" value="WHD_ORC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14052">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14052:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04084">
        <property type="entry name" value="RecA-like_ORC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24882">
        <property type="entry name" value="WHD_ORC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Origin recognition complex subunit 2 RecA-like" evidence="7">
        <location>
            <begin position="123"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Origin recognition complex subunit 2 winged-helix" evidence="8">
        <location>
            <begin position="439"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="219"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="32"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="9">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU368084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ81026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="509" mass="58289" checksum="D7D6507736617066" modified="2017-01-18" version="1">MQLDEVNVNKVRLNPDVKFVPTRVPENYSPPDDLTTTDGGTGASTASSAAAIAMPNAFIDGFEGYIDQSRMLWSQTSKTLISNNSMSMAPTVTRNEYDAVLRELNQLPTXDNNYKDGIDDSIEIKKKVYPQYAFELKQDFTLLFYGYGSKRNFLKELCEQYLSFNLNSIDGVLNMDDLKSEAIPVLHINGYNLQLSHRDIFAEVMKYIQVDDQELMSVLEPQSKNTETXEEDVKNDESXYDFDAPPKSKKRARETSKENKIWGNSINYELENLIKYYNITNSSFDKTFKLIVCIHSLDGPQPRRAKFISFLKLLAQINCIALVCSSDHMKLSLLYDHNAAVAFNFVYHDATNYRFYALEHSFQYDSFQTVYHDTQYVLPSASSGGMAVGSKKTTDNSNTLTGVKYVLDSLTKNSKKMYKLLLETILARKEKQSGRFNKAYRIEFKPFFDLCRDEFIVSNEMALRNMLREYVEHRMAAVTKNKRSGVEAIDVPYSVLEIDMILKEHMSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2C2</accession>
    <name>A0A1E5R2C2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Vacuolar protein sorting-associated protein 53</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3918</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the VPS53 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2C2">
        <property type="interactions" value="796"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2C2"/>
    <dbReference type="InParanoid" id="A0A1E5R2C2"/>
    <dbReference type="OrthoDB" id="10261632at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.110">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 53, C-terminus"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039766">
        <property type="entry name" value="Vps53"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031745">
        <property type="entry name" value="Vps53_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038260">
        <property type="entry name" value="Vps53_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007234">
        <property type="entry name" value="Vps53_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12820:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 53 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12820">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16854">
        <property type="entry name" value="VPS53_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04100">
        <property type="entry name" value="Vps53_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vps53 N-terminal" evidence="8">
        <location>
            <begin position="8"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Vps53 C-terminal" evidence="9">
        <location>
            <begin position="664"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="723"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="879"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="730"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="879"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="10">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="10">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ81057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="899" mass="104182" checksum="34622B2DE5A4E851" modified="2017-01-18" version="1">MVSISDFDYSPLDDLCEILKTPQALQELEGLIAKTTQYKQFLLHKEVTVSNEGAEKKELNEDEHTSLEEVYAAMKSINKQYNEYLLKLDSTQASIMSLTSYISELDCLKTNLQTSSQFFGNLKSLCEHYTNCTKILNNNSKTKDEEDYISMVEXXKVMDKLASDYFEKEYKSINEINRMLIMVSDLKETLDSRFKRHFSQLLDXDPSSGPASGLSQDQFKNGVCEIIGVNQRQKSEIIHWIIDNKVLFEFKQVFQIDDETSSLENLSRRFLFFKKVLNNFQSKYAEYFPANWDMLLEITKKFYQLTRTDLIIILKRDLTHQQQQQQQSXSININVFMESLQATLEFEKYIKVKFNNRLNKESDVQNISTCFEPYIYLWIDNQNPILQKKIMQYMSEPKIPSTSEIEDTSNASIIPSSMDLFRTYRQILNQALELVSSSDNESKDKMILQLTKFFNKWLLEYLNKILKNLLLPESPTSGASAXAAASAADADADGADKESIIQYTLLLINTADYCSTTINDLAEKLNEYCPNSAATIDTQLQSCKDQYLLVINSGIAVLLNRIIVPELQFVWKEFNNINWAHVQIEDYSRYMGSLEKILNNGSLPQIMKHFYKELYIWNFWDKIVNYIMETYIASIIKLLNLQQWKIDPAVKPKFSISQVVSIGEQLQLDTELLKKTLYTIQEQEFTAQPSSSSLKRATANIDSSVSLIVEFCKLLTTPVDSTAEPAAAXPREENKSNVDDINDDKSSKEESPKDETIIQESQYNQIFKKLTHNCINPAVWAYVLVLKGCPWDLSLWSSLWMQGLQEGNLGDWFIFKTSSVKHMFIQFNNFLYSVPNNNQEWYTFIHQDLQIKEFKQSASSRQVPQKQFSLQQQQQQQQQQQQQQQQSNLQQKLESLFNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2D4</accession>
    <name>A0A1E5R2D4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative oxidoreductase TDA3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g4139</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2D4"/>
    <dbReference type="FunCoup" id="A0A1E5R2D4">
        <property type="interactions" value="122"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2D4"/>
    <dbReference type="InParanoid" id="A0A1E5R2D4"/>
    <dbReference type="OrthoDB" id="498204at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF150">
        <property type="entry name" value="OXIDOREDUCTASE TDA3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="3">
        <location>
            <begin position="26"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="3">
        <location>
            <begin position="293"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="334"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="247"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="347"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ81066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="575" mass="63100" checksum="576E7419BB3226B7" modified="2017-01-18" version="1">MTDSIDDIEFSKTPNFAKILKSSEHIVVVGAGIIGVCTAYYLTRHPLFDRERHHITILESKRVAGGASGKAGGLLATWAFPQQIVPLSFQLHQDLSDEYDGDKHWDYRRLTTVSLEADVREKEQVGEGEEEEEEEEEEEEENTNSDDGSNSSTKSKSKKLEGLNGLKENEIGCCLMKNSAEQSVMDFESVEDVDDDRGDSRYNDSSDDEDDDDDDSLADFDNHDFVPGAVSMTSGQQASNTNSASGNESKNNNSDNCSDNKESANSKKKLPSDLYWINHSLVKGWSSLGGTDSTAQVHPYKFTYFLLKKAMETGAVDIILGKISKIHYSDVADASTNKQSQKKKSNEPTTDTTPSLATGVSYIPTDDSVLEELNGAAPQEVAMDATQVVLCMGPWTSKILPDCPISGLRAHSITIKPEQGTVSPYAIFTELQTSENEFFSPEMYARKDEVYVCGEGDTMVSLPETTADVQVVREKCDELFNYVSKLSTHLSRGHVLKRQACYLPVLNVPTSSGPLIGETNVENLFVASGHSCWGINNAPVTGKVLSELIYEGESKCCDISALDPGLYFDAQEQHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2I4</accession>
    <name>A0A1E5R2I4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3925</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2I4"/>
    <dbReference type="STRING" id="56408.A0A1E5R2I4"/>
    <dbReference type="InParanoid" id="A0A1E5R2I4"/>
    <dbReference type="OrthoDB" id="4097093at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184606" evidence="2">
        <location>
            <begin position="29"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="150"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ81115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="339" mass="33912" checksum="F3C86402A634E30E" modified="2017-01-18" version="1">MQLTAYTSKKNTAVLVLALMAISQQASAAESNSSDDSGSLESSSNSNLGADSSANSSADSKASTASTAISVATSNINTLSASSVEVVSSAATTTGATTGATTSGTATAQFAVEPSTTILTTDAQGQTTTEYLWYVPNDSSASASASASASASLPTSDTSSVIASTSNSASASTSQTKDNTKTTTTLSTTAASSTGSSRAWDEPLTTETVTLSNGDVTTSHIWWLASTTSGGSSSASDSVSSSTGISDVSFSTSYSVSTYTSNDMTYTTSIAETTLTTKNSNKKSSTSATVSSNSTSNAAGSLFHYRASKNTESNAFQTDALWNVAGVTLSCLAVVGLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2J1</accession>
    <name>A0A1E5R2J1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Suppressor of disruption of TFIIS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4343</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2J1">
        <property type="interactions" value="445"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2J1"/>
    <dbReference type="InParanoid" id="A0A1E5R2J1"/>
    <dbReference type="OrthoDB" id="1065058at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008252">
        <property type="term" value="F:nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009166">
        <property type="term" value="P:nucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006206">
        <property type="term" value="P:pyrimidine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010237">
        <property type="entry name" value="Pyr-5-nucltdase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052791">
        <property type="entry name" value="SSM1_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01993">
        <property type="entry name" value="Pyr-5-nucltdase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47438">
        <property type="entry name" value="PHOSPHATE METABOLISM PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47438:SF1">
        <property type="entry name" value="PHOSPHATE METABOLISM PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01132">
        <property type="entry name" value="C1.5.3:_5'-Nucleotidase_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="431" mass="49519" checksum="D3B2BEE0426BA846" modified="2017-01-18" version="1">MIMTASNTEKQPLKNKLNKMDSLTRTLSTTSSNSNSISTNRSRSNSSSGNIDAFSNNYDENNVGGESGEGGVVVLQHGSAAGADPGYYTTTNTSTSPDSNSITINHNYAVPRNIHKYNYQHLIQLIVNDDIPFDEYKKQIFLQLIDNEENLRYIEKTQVGTHCPKLMECLRMEERAKSNRFQKSSNYSTPLQTYTTKPCDSVFFFDIDNCLYKKKLEIHVLMQVYIRKYFQNILKLDSYKDAKLLNEXYYKEYGLAIKGLINENPHIEIDPIEYNKLVDDALPLQDLIKPDMELRRVLVDLKKSGKVDKLWLFTNAYKNHGIRVVKLLGIADLFDGITYCDYDKEENMICKPSLKAYEKAKLQSGLRDYRNAYFIDDSINNIKTAKSLGFKNAVLIDEDYQSDDRNVEIEGKQFTVVKDFEELKEVMPELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2T0</accession>
    <name>A0A1E5R2T0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">NADH-cytochrome b5 reductase</fullName>
            <ecNumber evidence="15">1.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g3891</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2 Fe(III)-[cytochrome b5] + NADH = 2 Fe(II)-[cytochrome b5] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46680"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14 15">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.6.2.2" evidence="15"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2T0"/>
    <dbReference type="FunCoup" id="A0A1E5R2T0">
        <property type="interactions" value="328"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2T0"/>
    <dbReference type="InParanoid" id="A0A1E5R2T0"/>
    <dbReference type="OrthoDB" id="432685at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090524">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NADH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06183">
        <property type="entry name" value="cyt_b5_reduct_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000032">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000009">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001834">
        <property type="entry name" value="CBR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370:SF171">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00406">
        <property type="entry name" value="CYTB5RDTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14 15" id="KW-0274">FAD</keyword>
    <keyword evidence="4 14 15" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="10 15" id="KW-0520">NAD</keyword>
    <keyword evidence="9 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="16">
        <location>
            <begin position="56"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601834-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ80883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="312" mass="35041" checksum="05591A47A384BD9F" modified="2017-01-18" version="1">MLSRLSHSKLFYPSIIAASAVTLYATRNHFMNTSNTNNNFLSNDAAPSNAVFTGDGKWIDLPISKIENLSADSRRFTFKLPQDDQVTGLITCSALLAKYVTPKGSNVIRPYTPVSDIHKTGEMELVIKHYDNGKFTSHLFGLDVNDTVSFKGPIPKWKWQPNMFRNITLIGGGTGINPLYQLIHHVCVNPQDNTKIKLFYGNKTPQDILLRSELDALAKKYPDQFQVQYYVDQESTQAGLQQNDVLKKGFIGKDDLRSKLPKPEDQTHQIFLCGPPPFMKAYSGEKVSPQDQGQLTGILSELGYSKDQVYKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R3A9</accession>
    <name>A0A1E5R3A9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">4,5-dioxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3911</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R3A9"/>
    <dbReference type="OrthoDB" id="9970095at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1240">
        <property type="entry name" value="DOPA-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023389">
        <property type="entry name" value="DOPA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014980">
        <property type="entry name" value="DOPA_dioxygen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36423">
        <property type="entry name" value="AFR070WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36423:SF2">
        <property type="entry name" value="AFR070WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08883">
        <property type="entry name" value="DOPA_dioxygen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143410">
        <property type="entry name" value="DOPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="unsure residue" description="D or N" evidence="1">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ81053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="15970" checksum="3C7B76D3BB1FB322" modified="2017-01-18" version="1">MSHETSHFAKFHYNIKSYDFHTYFHLNDKEETKFAIIGFRENVIEEFKDDIAHKKIGNYXIWHEQVGPHPSGYGMFEFDVRDSNSFLKLLNFYQLNHGQLSVLIHPKTNQGDLFDHTQGALWLGPQLDLNLDTLMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4U4</accession>
    <name>A0A1E5R4U4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein transport protein SEC23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">AWRI3579_g3508</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15 16">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The COPII coat is composed of at least 5 proteins: the SEC23/24 complex, the SEC13/31 complex, and the protein SAR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 16">Cytoplasmic vesicle</location>
            <location evidence="1 16">COPII-coated vesicle membrane</location>
            <topology evidence="1 16">Peripheral membrane protein</topology>
            <orientation evidence="1 16">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 16">Endoplasmic reticulum membrane</location>
            <topology evidence="2 16">Peripheral membrane protein</topology>
            <orientation evidence="2 16">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Golgi apparatus membrane</location>
            <topology evidence="16">Peripheral membrane protein</topology>
            <orientation evidence="16">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the SEC23/SEC24 family. SEC23 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4U4">
        <property type="interactions" value="1070"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4U4"/>
    <dbReference type="InParanoid" id="A0A1E5R4U4"/>
    <dbReference type="OrthoDB" id="10256289at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11287">
        <property type="entry name" value="Sec23_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.730:FF:000005">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.380:FF:000001">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000006">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000008">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037364">
        <property type="entry name" value="Sec23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037550">
        <property type="entry name" value="Sec23_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141:SF0">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14 16" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8 16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 16" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="12 16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 16" id="KW-0653">Protein transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0813">Transport</keyword>
    <keyword evidence="9 16" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="18">
        <location>
            <begin position="53"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="19">
        <location>
            <begin position="121"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="21">
        <location>
            <begin position="405"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="20">
        <location>
            <begin position="531"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="17">
        <location>
            <begin position="644"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU365030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="OEJ81937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="776" mass="86839" checksum="7CFACDFFFD1FE9C9" modified="2017-01-18" version="1">MDFDTNEDINGVRFSWNCFPATKSDANRNVVPIGCLYTPLKEREDLAIVNYNPVLCTGPQCKAVLNPYCQVDMRSNTWICPICKNRNHLPSHYTGITQENLPVELSNTTMEYILTNKQVNVPPIFFFVVDLTTEXENLTSLKESIITSLSLLPPNVLVGLITYGNTVQLHDLSCKTIDKCNVFRGDREYELNHLIELLTGEKTSATSGTGAAGLNNVMMNGMSKITPYSLNRFFLPLEEIEFKLTQILENLQPDPWNVAPGKRPLRSTGSAMNIATLLLESCYKNCSSRIILFSSGPATIDPGLIVSDELKDPLRSHHDIDSDNAKHYKKAVKFYTNLAQRAAKNGHTVDIFAGSYDQVGISEMQNLTDTTGGVLLMTDAFSTAIFKQSYLRLFSKDVEGYLTMGFNGSMCVKTSKDLKLQGLIGHASQEKKTDAWNISENSIGLGGTSTWKMSSLTPQHTYAVFFEIANNNPNSNGMSNGMMNPNEPGPLAYTQFVTSYQHSSGTYRIRVTTVANQLLPFGSPLIASSFDQETAAVLMARIAVYKAEKDDDADVIRWVDRTLIKLCQSYSDYNKGDPSSFRLTQNFSLYPQFMYYLRRSQFLSVFNNSPDETAFYRHVFTREDCTSSLIMIQPTLTSYSMEEDPQPVLLDSISVKPNTILLLDTFFYILIYHGEQIAQWRKAGFQDDPQYADFKALLQEPKLEAAELLVDRFPLPRFIDTEAGGSQARFLLSKLNPSDSYTNQQATAGSTIVLTDDVSLQSFMQHLQQVTVNGQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4Z2</accession>
    <name>A0A1E5R4Z2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3798</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4Z2"/>
    <dbReference type="InParanoid" id="A0A1E5R4Z2"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="434"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="303"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ81972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="483" mass="55637" checksum="E5894FFB9D2ACE86" modified="2017-01-18" version="1">MRRLVERHETDLADSASIQLSTPDLGPLTRDAGHTPPRSAASSMHRNIHFLVSNDNDEREPINYEIVENSEAERSLSSVHTIVTRENNGSDFSTSLRWSWSQFFQRYAQENERFRPIHQDQNGNNDLQIKRQENRHRRLRHFYNWRHRHSERENRSLDISSIGTGQTGFERQPGDVVSFLKANNIVQVIGKEGKLSWETWQKLALLIDRICPLENWDHYSSPANNPEIKRIVEMIIQNFHVAVLENDLDAFAKYLASYAIMVSEHKDKNEFEISHAHDLAKPFAHHSLMGVELLEKTEKEIMKNQLQFQNEKLADKVKALEKNLLDLNEVLDEQADLIKKNITLISWQKHKLQSLAVSRKKSYKMVATMTSQLKNILHSQASPSPAIENTARSCNSIMQRLKKSDKQKGKTLTGSEFDMFLTSVFEPTIKHLTNSKQRANHTRKTSKSFQNPPFSDVDSVTLGVQQLSMCEKDPLENCHTAVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R500</accession>
    <name>A0A1E5R500_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">37S ribosomal protein MRP2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3522</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R500"/>
    <dbReference type="FunCoup" id="A0A1E5R500">
        <property type="interactions" value="561"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R500"/>
    <dbReference type="InParanoid" id="A0A1E5R500"/>
    <dbReference type="OrthoDB" id="413436at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1480:FF:000001">
        <property type="entry name" value="30S ribosomal protein S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001209">
        <property type="entry name" value="Ribosomal_uS14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19836">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19836:SF19">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US14M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00253">
        <property type="entry name" value="Ribosomal_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00076896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ81980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="113" mass="12782" checksum="EAEF78499221A034" modified="2017-01-18" version="1">MSSKINYLRLPPLFVKAGIIKDNYVRHAFAKTEVTQQALKHIARNTTLPTKVRLQAQLQLASMPNYTKFTQVKNRCVESGHARGVISKFGLNRYVFRQKALAGELPGVKKGVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R520</accession>
    <name>A0A1E5R520_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-(5'-phosphoribosyl)anthranilate isomerase</fullName>
            <ecNumber evidence="3">5.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3734</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TrpF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.24" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R520"/>
    <dbReference type="FunCoup" id="A0A1E5R520">
        <property type="interactions" value="168"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R520"/>
    <dbReference type="InParanoid" id="A0A1E5R520"/>
    <dbReference type="OrthoDB" id="524799at2759"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00042"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00405">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00135">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001240">
        <property type="entry name" value="PRAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044643">
        <property type="entry name" value="TrpF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42894">
        <property type="entry name" value="N-(5'-PHOSPHORIBOSYL)ANTHRANILATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42894:SF1">
        <property type="entry name" value="N-(5'-PHOSPHORIBOSYL)ANTHRANILATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00697">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="N-(5'phosphoribosyl) anthranilate isomerase (PRAI)" evidence="10">
        <location>
            <begin position="5"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="N-(5'phosphoribosyl) anthranilate isomerase (PRAI)" evidence="10">
        <location>
            <begin position="181"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="116"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="119"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ82000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="293" mass="32850" checksum="884FFE9D673F4473" modified="2017-01-18" version="1">MEVVKVCGLRKLEDIEYCAENGNGKITHLGIICVPGRKRSIEREVGLKISQVLDKCTTKDKRPKLVGVFRNQPLNDIIALTKEYRLDAVQFHDDNPEWLEWSLELLNIQKNNPLFEKASSSSPSSSPSSSPSSSPSSSPSSSSSSSSSSSSSRTNTYPPFSIIKRWCFPRDRMLIVQYAKKISEMNLQNHFIFLFDSETGGEGKVSNDWKAVSDFLQEIRKNKNISTGLKFVMAGGISPENVQILKKTCPEINGMDVSSGVENEKTGWKDYEKVERYVAEASMKDGEYRETEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R565</accession>
    <name>A0A1E5R565_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA polymerase-associated protein CTR9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3837</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R565">
        <property type="interactions" value="1006"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R565"/>
    <dbReference type="InParanoid" id="A0A1E5R565"/>
    <dbReference type="OrthoDB" id="343875at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031101">
        <property type="entry name" value="Ctr9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14027">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN CTR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14027:SF2">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN CTR9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="233"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="780"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1012"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1012"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1059"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1092"/>
            <end position="1113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ82055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1128" mass="129519" checksum="2F10E0DD8B103CDB" modified="2017-01-18" version="1">MSQTVFDTNVSSGGSQEKFAYPVIQWDTTFDIPLKDSDEVVSIDLVNDLPPDPNELTSLLIEENANREHWLRIAIAYCNQNKLKEGIKLIELALDVFQGPLSSQLHTFLTWAYLSLTKKCGLDQVDNRKVYLQKSEQHLKSAIDYEPTWIGNMLATIDIYYQRKQYDKALETAQLFSKAIVSSQPQNSTKSXQQRINVLFLLLKAKVYYQKKNYTASLKLFQELLILNPVLQPDSRIGIGLCFWQLKDHDMAIKSWKRSLELNPNNEAVKILVLLSEFHNSLHKSGNDTEFFDSYTKYLQDLNSIFETAQKTHDEQVANSKEFPEYQQVEEVSPVLYCLLQNYFYLKGDHNKVVDIYNKVLPFKTLVTKDLSSEISFWCGRAHYALKDYKSAFQMFQESLKNSDEHFLSRFGLGQSQYENNLVEEALLTFENMYKTNESIQELNYVLGILYSSKIIGLDTTKSQSSSTGNNKSGAQNEDVMNKAFFFLEKYIRLTRAKKNQLINLNAYLVLSQLYEIKNLYDKSLHCLNKVVEQLEYMNETIPLEVYNNMACYXFSLSNMEKAQEFFGKAKDASAESISALTIDYNIARTSEETDGTVDFAVDSYTSILKTNPSYITARMRNIFLDYVKGERGEKLESEIEKLMSQDSSDLEVRSLYSWFLKNVSSKKVDDATGELLESKHNKETLTEFDSHDMYALISLANVYVNVARNAKRXSKKSPKEFERIKHSYIKAVHLYSKVLTLDSYNVFAAQGLAICLAENGMYTHALDLLKKIRDSLNVKSVFMNMGHCLTELGEYIKAVDSYSEALANCEDDVERSDVYNLLGRVWLLKGLKESNLEFIWKSLEYVEKASSNDVQFDKFKANLKYNRASLFITIGNMVCKLEPIKRSLKDLQKVDENLLACANDLNSILEDAELVKFFSREDLKHKCALLESSLKEKIGDSIQEQEAFEGGVVKRLEEARHHILAEEERSVAELKAQEEQDKLRKQKDAEEFKKLQEAAQKLIQERAEMIQNEDDKDQDLPIEEDGEFDEGKTSSKRKRGSKDSASKKKKQKKRARIEDDEEEEEEYYDDEEVPSSKSRSKKSTLSNEFINDSDVDADSNPDDLFGDEDEGEGPLKEASEPAPVEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R577</accession>
    <name>A0A1E5R577_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ras modification protein ERF4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3777</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Interacts with ERF2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERF4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R577"/>
    <dbReference type="FunCoup" id="A0A1E5R577">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R577"/>
    <dbReference type="InParanoid" id="A0A1E5R577"/>
    <dbReference type="OrthoDB" id="5377273at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031211">
        <property type="term" value="C:endoplasmic reticulum palmitoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019383">
        <property type="entry name" value="Golgin_A_7/ERF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051371">
        <property type="entry name" value="Ras_palmitoyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13254">
        <property type="entry name" value="GOLGI AUTOANTIGEN, GOLGIN SUBFAMILY A, 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13254:SF0">
        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER 7_ERF4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10256">
        <property type="entry name" value="Erf4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Golgin subfamily A member 7/ERF4" evidence="8">
        <location>
            <begin position="112"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="32"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ82051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="261" mass="29295" checksum="5ABCD053ED337195" modified="2017-01-18" version="1">MSSSDDSSHEVLELHHAPQKQETRETNEFVDETQTQSQASMNSPTSKERENSNDALAVLFNYHEYTVDEYSDGMGSAPYKHRVDHQDGTGSVQSHVLCINHFPSYKNKGKRIVRVPRISNYPIVFSTFIPGTEPSAVTTDFQFVKRGVYNGQWYNDSSTSPLSLYLSPQDFKSIVQHINALSVAATQGNGLLNFVMTFIDCVTFGLWTLLLTQFGYHPYRSIKSFVEKLNSENNTFADNGIALISPTENGFLSLDFEINAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R596</accession>
    <name>A0A1E5R596_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3714</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R596"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="209" mass="23891" checksum="4859F74415F7F4BC" modified="2017-01-18" version="1">MKRRNGEQEXYTFLSKEKLEEYKKSRNPDAKSRPTLXFERDFRKTHHAENAPKLVFKPWSSGGTPTNRDSKKFGEPRFDVADSSNPVRFQSDFKLEEYGPEICGYRFVGVSADPLQSEYPSDLERANLASAVDHILVAVAEPLDDISRDASRVREYLQAQNLYTQVHLKSLRVLLHPDKNVLSRGSASSARAYTLAFQLVDQLYKELXS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5H2</accession>
    <name>A0A1E5R5H2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3625</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R5H2"/>
    <dbReference type="OrthoDB" id="5361617at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="390"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="404"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="2">
        <location>
            <position position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="436" mass="49725" checksum="D4D00C5812C95984" modified="2017-01-18" version="1">MLPDDIAVXKERTKDKKNDESVVLENSGHDSRIDPPVFNSKTPESKRKNGKPNWKRAVKRDETTSGTPKTTALKESSTPQKPAKRKSTKKRKQRKAKTSGIDSNELPRKDDQELIFIVDPTCFTKGLGHVMQWVSYIEYMQKNHDSEAKSSSGSSVKRSNTSAAQKTKITLYLSTYTIEELNFLKTNRNFTSAIKSLKFIDTKFEYFDMEILDKKSVIEEMSWSGVLRDLFFPAEQSAPPLDDLENVGFQSDLSETQDHLETEESADAFDAVVDKPYQRQLVKKLFQATMKIPMRYKKLIKSYHLLQKELLQRSKEDPKVFDNTKVYIVSADIYVLQDLNQIFNGAVTTDSEISESDQQDSKKIEILDILKADIMIDDIYHSKSHKVLGAEKGSSQRSSNGFGSHNNVQNNNASSENMYFKKNFFAPRGKGELWTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7C1</accession>
    <name>A0A1E5R7C1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">E1 ubiquitin-activating enzyme</fullName>
            <ecNumber evidence="4">6.2.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3484</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="6.2.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the ubiquitin-activating E1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.45" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7C1"/>
    <dbReference type="FunCoup" id="A0A1E5R7C1">
        <property type="interactions" value="1296"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7C1"/>
    <dbReference type="InParanoid" id="A0A1E5R7C1"/>
    <dbReference type="OrthoDB" id="10252231at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004839">
        <property type="term" value="F:ubiquitin activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01490">
        <property type="entry name" value="Ube1_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2660:FF:000001">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12550:FF:000001">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000015">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.60:FF:000002">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.80:FF:000001">
        <property type="entry name" value="ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.180">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, FCCH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.80">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12550">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2660">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, SCCH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.60">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, UFD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032420">
        <property type="entry name" value="E1_4HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032418">
        <property type="entry name" value="E1_FCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042302">
        <property type="entry name" value="E1_FCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018965">
        <property type="entry name" value="Ub-activating_enz_E1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042449">
        <property type="entry name" value="Ub-E1_IAD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038252">
        <property type="entry name" value="UBA_E1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019572">
        <property type="entry name" value="UBA_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042063">
        <property type="entry name" value="Ubi_acti_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018075">
        <property type="entry name" value="UBQ-activ_enz_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018074">
        <property type="entry name" value="UBQ-activ_enz_E1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033127">
        <property type="entry name" value="UBQ-activ_enz_E1_Cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000011">
        <property type="entry name" value="UBQ/SUMO-activ_enz_E1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01408">
        <property type="entry name" value="Ube1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF4">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16191">
        <property type="entry name" value="E1_4HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16190">
        <property type="entry name" value="E1_FCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09358">
        <property type="entry name" value="E1_UFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10585">
        <property type="entry name" value="UBA_E1_SCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01849">
        <property type="entry name" value="UBIQUITINACT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00985">
        <property type="entry name" value="UBA_e1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00536">
        <property type="entry name" value="UBIQUITIN_ACTIVAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00865">
        <property type="entry name" value="UBIQUITIN_ACTIVAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-activating enzyme E1 C-terminal" evidence="11">
        <location>
            <begin position="918"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="9">
        <location>
            <position position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ82797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1048" mass="118126" checksum="C638F0DCF79BFEC5" modified="2017-01-18" version="1">MSQPKDKEIDESLYSRQLYVLGKEAMLQMQKSNVLIMGLNGLGVEIAKNVALAGVKSLELYDPNPTVLQDLSSQFFLTQQDVGKPRDQVSMIKLKELNNYVPINVASPNLLQNYETELSKYQVVVITSDLVSLKEIVQLNDFCHSKNIKFISTKSNGLFGQVFVDFGENFQIIDPNGEELKTGMISDIENDGTVTTLDATRHNLEDGNYIKFSEIVGENSDFLNDQSNIFKIKVLGPFAFKLVAEYNNNNNNNDQDGIASDIEGGKSCIPANFKYSKNGNFTQVKIPQTLSFKSWSQCYKEPEFFYPDFAKFDRPAQLHLGFQALDKFKDLHQRLPHPMNEQDASEFIKLVDQFAKEQPNVLQGNELSEKVLKELSYQALGDIPAMQAVLGGLVAQEVLKACSGKFTPIKQFFYFDSLESLPSPESFPRNENVCKPINSRYDNQIAVFGIDFQKKIANLKVFLVGSGAIGCEMLKNWALLGLGSGPEGQIYITDNDSIEKSNLNRQFLFRSKDVGKNKSEVAAEAVVAMNPDVRNKIVSSIEKVGPETEDYFNNDFWENLDFVTNALDNIDARTYVDRRCVFFKKPLLESGTLGTKGNTQVVIPNLTESYSSSRDPPEKSIPLCTLRSFPNKIDHTIPWAKSIFQGYFTETPENVNMFLDQPDYVEQLSKQAGDVKGILESIVSAFKDRPYNYEDCIKWARLEFEKKFNHDIQQLLYNFPKDAVTSTGAPFWSGAKRAPTPAEFDIHNKDHLDFIVAAANLRAYIYGMEGGNEGDRPDVSRYARVCSQIDIPHFNPKSNLKIQINDEDPVEKTPMNETRDEIDELISELPDRASVGSFRMHPVEFEKDDDTNHHIEFITAASNNRAVNYGIEPADRSKTKFIAGRIIPAIATTTALVTGLVVLELYKVADGKTDIEQYRNGFVNLALPFFAFSEPVPSAKGKYNDTEYDKIWDRFDIKGDITLQSLIDYFENEHGLEITMVSQGVSLLYANFFPPKKLKDRMNLPITQVVKLVTNKEVPANTKTLILEVCADDKEGEDAEVPYCTIYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7K5</accession>
    <name>A0A1E5R7K5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ureidoglycolate lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3341</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(S)-ureidoglycolate = urea + glyoxylate</text>
            <dbReference type="Rhea" id="RHEA:11304"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57296"/>
            <dbReference type="EC" id="4.3.2.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7K5"/>
    <dbReference type="FunCoup" id="A0A1E5R7K5">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7K5"/>
    <dbReference type="InParanoid" id="A0A1E5R7K5"/>
    <dbReference type="OrthoDB" id="10266039at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004848">
        <property type="term" value="F:ureidoglycolate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050385">
        <property type="term" value="F:ureidoglycolate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000256">
        <property type="term" value="P:allantoin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20298">
        <property type="entry name" value="cupin_UAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.480">
        <property type="entry name" value="Ureidoglycolate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047233">
        <property type="entry name" value="UAH_cupin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007247">
        <property type="entry name" value="Ureidogly_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024060">
        <property type="entry name" value="Ureidoglycolate_lyase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21221">
        <property type="entry name" value="UREIDOGLYCOLATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21221:SF1">
        <property type="entry name" value="UREIDOGLYCOLATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04115">
        <property type="entry name" value="Ureidogly_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ82876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="185" mass="20339" checksum="BCFCE83FD464E62F" modified="2017-01-18" version="1">MVTIKAEPLTVESFKPFGSIISPSEQLTIADTNANQGTAIKQRKVSALTNGFATTSNVHAKDHQINFNIFQCFPQIQLAQQNSHVCKILEKHPFSSQTFIPMCGDAQNEKQYLIIVANGTDKPDLSTVKAFFVKGDQAVTYGMGTWHAPMIVVDKTVTFAVAIAECDVDELDCVEDPSTEITVEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7P9</accession>
    <name>A0A1E5R7P9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3188</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R7P9"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="41"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="606"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="607"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="630" mass="71148" checksum="7C43462D87EB1544" modified="2017-01-18" version="1">MNXXLFTLPSWQPQQCDDLEKHGLDYNANXXLEANPQYVGKTSLDKLPLAVLFQICLYLDFNDICNLCCANKLLKGIGNEALNYKRKYNIRSPNCGLISRKWEEKLIYAALKVLEPHKLALSNIQSKGVFIVDPLLAIQQRLMRESSEYINMITNEEEIDGHKNDTTNELEHAKKCAGESEVEECILEQDFSELSKNLAYINRLKLNDFDLSRGSGDIYMNATSDANDSNDNSTLLGNNNMNGSISTPKLLDFEENGENSGKFEINMQNDQIILPENNFLDELHFVNSIPDTALSDSITNQENSDRRLHNVSRPDFSDTTENCTNDIFDKRVRERRNIFESGAPIGFIGNIPAQLPTNSYEMAFEEIQEETCGKILAGLEELTDLSKTMSTKAKIKMFESASQQTTXPLQKTASLKESTNKQDMHXAELGEVKKIESTARNINTKLFQNTFXFSSVVQPSEKEEDMQDXDSYSNSSFELIRQLQMSTKVKDKRMLFETLVEKTNELSLGNSKYRKHSKKKRLTERVFSNNSSIQHTNNEXIXELETPAQCSLTESPPRSSTAVEQVDLSLLDDFSDEKEKLEIKSLPQSSSFDAYSNLDTTVAVAKNETANETANETANETANETANETE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R826</accession>
    <name>A0A1E5R826_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R826"/>
    <dbReference type="FunCoup" id="A0A1E5R826">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R826"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="444" mass="51481" checksum="B19DCCF648307BCE" modified="2017-01-18" version="1">MEVALLRLPRVSFSLHENKLISLLNKMKKSSRSYTTVTPTTTNSAHSQLNLTNKNDLPVKTSSTIPAGSASFNKTVNVPLSQYISFQHFKMVDGREDMAQYVKDVPYLPIFQYPHEITNTKNISRWKQIFKKGKFILKYYFYGIKNILVYLKNNKLMTSNHKNIGRDTNDLVKKIEFKLMEIVQENQRNPSIGTEATKDLSVKQLSTINEHIFAVSNVLSSRRIFEQRSMHFLNGDLLRFVWFSGIVFVFEELAILLFLFCIPSRWKHKVIPYNCTIPELLASQANANLQAFHSRVLQDYSKDYLANDYLSPPKLAIELKKTPANLAPNKNVLSSAFFVPQIDEAYMKYIYLDDLLILRDMVESHRNAPMQCWYKEELVQKCCERFLVKSLSASKELDVASLQQKLCAYLLEKYAQDLDVNKKFGIWNVYTLGRIPEEYKDQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R852</accession>
    <name>A0A1E5R852_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GPN-loop GTPase 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3457</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Small GTPase required for proper localization of RNA polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Binds to RNA polymerase II (RNAPII).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R852"/>
    <dbReference type="FunCoup" id="A0A1E5R852">
        <property type="interactions" value="1065"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R852"/>
    <dbReference type="InParanoid" id="A0A1E5R852"/>
    <dbReference type="OrthoDB" id="5839at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17871">
        <property type="entry name" value="GPN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000338">
        <property type="entry name" value="GPN-loop GTPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030231">
        <property type="entry name" value="Gpn2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF3">
        <property type="entry name" value="GPN-LOOP GTPASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="312"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="337"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ83089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="354" mass="40548" checksum="90E6739E875D4989" modified="2017-01-18" version="1">MPFGQIIIGPPGSGKSTYCFGCQQFFNAIGRNCSVINMDPANDSLQYECALDIRDFVTLEEIMSDQQLGPNGGLMYAFESIENSLDVFILQIKSLLKEQNSYLIFDCPGQVELFTHHGALFKIFKKLEKEMDLRLCVVNLIDSIYITNPSQYISIVLLALRSMLMMDLPQINVFSKIDMLSQYGNDLPFRLDYYTDVDDLSRLQPELEKESEIQGAGSVGKKLAKLTASISEIIQDFNLVSFEVLCIDDKQSMISLQTMIDKANGYVFGSAEITGDTVWSEASRQGIIHDLGIVDIQERWLDNKEEYEKEEERKRQEFLKEQEMHDKPVNVDEEDEWNRAMKEWERETGNKAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R858</accession>
    <name>A0A1E5R858_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exonuclease V, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Defects in morphology protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g3284</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EXO5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R858"/>
    <dbReference type="FunCoup" id="A0A1E5R858">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R858"/>
    <dbReference type="OrthoDB" id="354769at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045145">
        <property type="term" value="F:single-stranded DNA 5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019190">
        <property type="entry name" value="EXOV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14464">
        <property type="entry name" value="EXONUCLEASE V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14464:SF4">
        <property type="entry name" value="EXONUCLEASE V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09810">
        <property type="entry name" value="Exo5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8 13" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ83074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="599" mass="70306" checksum="F567322A7A036C7B" modified="2017-01-18" version="1">MNHHGIRNLVCTTRFARLYSKLTEDEIRSLQSLPFLKNLDTTPQPLTPEQVSLLSVEESKILDRLPKKKREYINKKIATLKPIFRKGSSDMLDYRITDPAYSHVTPFDVESSKRLSVTKLLTKGWCELRKMYDIYAQTDIFKSKRVAEGSKIHSTLEERTHKKTKDVLEIIDSYNIENLLQEFNEKWLELEAGHKLLDVPSAEGEHVEVEEILDLELFAEDIFSQGWCNNVSRLYDLCTNGEAREILVHGYINKSTGKFVEGAIDSKSEDLILISGVIDHIYVPNHPTWDSSYSPLDGNHIDENDDMDATFKDIDVLLNHPPKETEAPWVYHLGDVKTRAYNNVPLQENVVNASKLQVMYYKFFFRNLCTQPREHTFQKLVHNAHYRGLDPYKYLQSEQVFSLFVQFAFFRKDLLKLKDGSIFQDEVLDGGSLQVLTQEANAAPEYDQEKLNYYLQRFFPDWGFEGKWVYWPTLEYFARRMSQLYSRVLSQPNSSISNELSIEYYNKRNMLFQKTEFEYDENYFKQNLESSVAFWLGKRLVEPIDPLVDDNLNTYCKKCEYRNHCFWKFKRMENLQKMSTKSKKLGTMLQEINRSLCES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA79</accession>
    <name>A0A1E5RA79_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional activator FLO8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA79"/>
    <dbReference type="InParanoid" id="A0A1E5RA79"/>
    <dbReference type="OrthoDB" id="5600002at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36911">
        <property type="entry name" value="LIM ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="549"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1176"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="284"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="318"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="554"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="692"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="715"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="753"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="771"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="858"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="868"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="896"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="958"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1014"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1176"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1193"/>
            <end position="1215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1233" mass="134949" checksum="0E675041E42DFE60" modified="2017-01-18" version="1">MDLNRIPSGFSPGYNKGKDNNEMNGQTAAEINISNNNTKDSDNTNPKKSNINNNNNNNNNNNNNNNDNDNDNNNSTNNNPNNNDNDNDNDNDNDNDNNSNTINSSKNGETEKFYEDLQRNSSLNTLGFADDDHFTRFLQETIGDNHDIITNEPIATGKDTNELIASESMVDETAINKVIKKEDHSNKEVDGSKLDKKNGEVNCNINNKDTKAKETIENPASIVNSNFXNIDTQENNXSLQNAKKPTTKQNSNNVNNNKRLNETFDDETASISSTKKRRVKKNNNDNGTTKIISNSNTSEISSANDAHKMEKPVQKVDSSTSNGNSSNNNNNNNNNNNNNNRTXKRNNNTNNTKXXTPPNATIDQTRQKQKXSNSNFSKPNAPKATKPLPHTHKDYQPDTPNKRLLNAYIYDFLIKSQLNKTAKSFVKESVVEERGKPPVLDPQGNFVSFDLTYNVPRGFFYEWWQIFWDIFNARTNRGGSELAQKYYKSELIRQKPEHYYRQAVIQAAHIQQLAEQRGEYHMEMIERQSLQNMQYPPYPMYMSGSSSYPSNHPYGPPQGFPHGPPHGPPHGPPHGPPHGPPHGPPQGPPQGPPQGPPQGPLHGPPHGPLHGPSYGPAVSFVPNKRQVPPSGAPRYYHPSQGIPKSVLPQGAPTGAPVGPPNGTSYETRGPAPGTYIRGKPGQISDVSMRPTSSSMSQTYSSGPYPPAQYPYGTYPGSNASVNRNTPSMTPFNDRSNTNNLVSQGGPSGTMSFQTQVEQQQQQQNIGQPQQNTPKAGNPQSMQQSYYPQNHPPPGPPQNLAVFPQQKKKRPVTQGSPLAETKFLGPEHQMDNIIKQEANSTAFPTKKFEKQSTTNGKSTSRKSSKSSSRRPSGSNPMGNPGGTLNNGTETNDADYGNTAQFPKSQNTTDKAKRSMMRPPSKTSSPRADATPSGNAAFALSPPTYQSMSKKIAKKKIANVSDNSRSATELAQKDTQQVNKYYSAPTTPASSIFPNSNASKKNGSNTVPPPPGTEPLSAQKNQGNGKESPGSAFFVPNVHTTSTTASTLANNANFMAKKQQRVYAKTPTVGGSPAVSAYSQALNAAPQXNNYFASPRSRPPIGENXTAAREHYDSMNTLHDGYNTQNGINGPQPPFPQENYXGSSLGGYPHDNDENLHMGDARMNXXGLNFNGFSPTHPTHAPPGTATATGPMHQKATNNMQPMHTYQNHKSTSSDSPVQELDVDGLNHFFNWDNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA91</accession>
    <name>A0A1E5RA91_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2938</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA91"/>
    <dbReference type="InParanoid" id="A0A1E5RA91"/>
    <dbReference type="OrthoDB" id="410198at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052564">
        <property type="entry name" value="N-acetyltrans/Recomb-assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43451:SF1">
        <property type="entry name" value="ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43451">
        <property type="entry name" value="ACETYLTRANSFERASE (GNAT) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ83791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="291" mass="33012" checksum="24A2FBA60B6428B5" modified="2017-01-18" version="1">MSLVNYTTDIAKVAETLNSAFMRSPSNLYVMKKIFNIKLDEKFSSHRNLAMMFYYASMYSDLGGELCESNNYNAVSLVSPPGVHIDFAETHDDLFNKIWYEDLDNSFKDLVGENPYYYICLVGRNFEQEKQKGSVTNIFKHYQKLADMKNIPIILESISEMSTQIYKHYGFKAIKKFKYGQGEVDENGRPDXNGQGFEAEIMVYYHDFDKLTKYRNSIVTVESLPLVDPKTYTYIPSSTTPSKNNTNVVDVVIPSSKAVDLSTGITCAPMSSGIVPNLTHHPVSIVSEKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAI7</accession>
    <name>A0A1E5RAI7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2574</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAI7"/>
    <dbReference type="FunCoup" id="A0A1E5RAI7">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RAI7"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="187" mass="21891" checksum="BA38CB384CCBEE08" modified="2017-01-18" version="1">MRARNIDKSVLDATREEYEMIDSDSVECDAEQDNTVMDTDAQEDLISDFRIKMMTSNKKYIKMLQIMIVSYILIILIVHPGKLKNKRNKTLSVQSSCISLLYLTGKYNTTKKQAMVFIIINFLLIGFLILKSFVSVENWKERLVVQIVYALPPLIFCSISQLFYYWSLDLEKNISKLIKQKYKYKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAV6</accession>
    <name>A0A1E5RAV6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">chitin deacetylase</fullName>
            <ecNumber evidence="12">3.5.1.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g2727</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Hydrolyzes the N-acetamido groups of N-acetyl-D-glucosamine residues in chitin to form chitosan and acetate. Chitosan is a component of the spore wall.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>[(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n) + n H2O = chitosan + n acetate</text>
            <dbReference type="Rhea" id="RHEA:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:9593"/>
            <dbReference type="Rhea" id="RHEA-COMP:9597"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17029"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57704"/>
            <dbReference type="EC" id="3.5.1.41"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:10465"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Prospore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the polysaccharide deacetylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.41" evidence="12"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAV6"/>
    <dbReference type="FunCoup" id="A0A1E5RAV6">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAV6"/>
    <dbReference type="InParanoid" id="A0A1E5RAV6"/>
    <dbReference type="OrthoDB" id="2125469at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004099">
        <property type="term" value="F:chitin deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.370:FF:000008">
        <property type="entry name" value="Chitin deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050248">
        <property type="entry name" value="Polysacc_deacetylase_ArnD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10587:SF133">
        <property type="entry name" value="CHITIN DEACETYLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10587">
        <property type="entry name" value="GLYCOSYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="3" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="10" id="KW-0170">Cobalt</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0749">Sporulation</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184674" description="chitin deacetylase" evidence="16">
        <location>
            <begin position="22"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="NodB homology" evidence="17">
        <location>
            <begin position="123"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00054095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00060373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ84036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="325" mass="36183" checksum="369F329844505032" modified="2017-01-18" version="1">MMTSLFAYTLVYLALLSLGYSAEINHSQALSAEKAQSARLVSGLQGALSVAAKTPFPKWLSDFTGLTEWPGLNPPYIPLDYIDFSKIPNYKPYTQGSCGLNPRIACSFDCDKCVEPDDVYTCTKLSQTFDDGPSDATQDLLNHLKHKTTFFNLGINVVSHPDVYKKILDKGHLIGTHTWSHPFMPSLTNEEIIAQIEWSVWAMNATGNHLPKWFRPPYGGIDNRVRAITRQFGLQAVLWDYDTFDWQMLGGGQTRKEADVYRDVAQWKSRTPAQGLVLEHDGSQKTVDVGKTVSDIIGPDQLTVAQCVNGIDYIKVFGQADNLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB45</accession>
    <name>A0A1E5RB45_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2931</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RB45"/>
    <dbReference type="FunCoup" id="A0A1E5RB45">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RB45"/>
    <dbReference type="OrthoDB" id="4036490at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012470">
        <property type="entry name" value="Pup1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07954">
        <property type="entry name" value="DUF1689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="332" mass="36837" checksum="64D6D3C9B22A0F93" modified="2017-01-18" version="1">MSSSDFPGNSNQGGNPPNPNNLVRLNDNTMASVGMSATGGEAIEPEDVRNPWLKEAYSAAIEFYENDKNLTKKDRQELSASFLGIARAQLYTGWSFFTIVFGTPFAVKYYKTKSVKGIAVPRNFIMGIAAMIFGTITGGKIAYTSAIDRLKNSTYEENIGDKSFRQYQILKLISPGESPKWAMYYKNTSLNPQRAVQDPRTLLHKVQNKLDQRGGQPPVSTFITQRDPLNLYSGPEFDQYKQDQKHNDYPSSLPSADSAPTLGSSYASSIEQPSQRSDSNLLNVKLDNPSSSIQKDHLGENDPDKDDPFFQENAESNSSGSAWDKIRRGKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB53</accession>
    <name>A0A1E5RB53_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL23m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2636</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB53">
        <property type="interactions" value="258"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB53"/>
    <dbReference type="InParanoid" id="A0A1E5RB53"/>
    <dbReference type="OrthoDB" id="3971233at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_uL23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12059:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL23M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12059">
        <property type="entry name" value="RIBOSOMAL PROTEIN L23-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ84129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="278" mass="31567" checksum="3BB3BB10EEFD7AAC" modified="2017-01-18" version="1">MQSLIARSTRVXLQSTAFITPVFKRCLATTTTSATTGTVEDTKVTFEPPLNEGLGAEPVKKIGGRKRPIKVAERIASLMEKAVEQDEPHFKVGGKLSYFPRELVTLLPPIAKHTPYQARFRVPLTFNKLDLRDYLYHIYGLRAYNITVQLTRPVFLAQPKGKKLTTSQQKIMTIDMDEPFIWPAKEFEEHQKDMLVEEKVHRDASLFIQEQAFRKGSDHFKPIKNLFDGVGGATEPIAQPFVSALSYRQLKNEKLNSAKLKQEKSKLDNIESLINRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBA9</accession>
    <name>A0A1E5RBA9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Actin-related protein 2/3 complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2480</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">Cytoskeleton</location>
            <location evidence="6">Actin patch</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ARPC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBA9"/>
    <dbReference type="FunCoup" id="A0A1E5RBA9">
        <property type="interactions" value="726"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBA9"/>
    <dbReference type="InParanoid" id="A0A1E5RBA9"/>
    <dbReference type="OrthoDB" id="336240at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1460.20:FF:000001">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034666">
        <property type="entry name" value="ARPC2/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008384">
        <property type="entry name" value="ARPC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22629:SF0">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2_3 COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22629">
        <property type="entry name" value="ARP2/3 COMPLEX 20 KD SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05856">
        <property type="entry name" value="ARPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039100">
        <property type="entry name" value="ARPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69645">
        <property type="entry name" value="Arp2/3 complex subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR039100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ84185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="172" mass="20266" checksum="84698B367A7A90B7" modified="2017-01-18" version="1">MSQSLKPYLTAVRYSLTAALNLSNFSSQEVERHNRPEIEVAHQSMELLLNPMHISRNEHEQVLIEPSVNSVRVSIKVKQSDEIEEILVHKFTRFLEQRAENFYILRRVPINNNYSISFLITNQHTETMKVNKLVDFIIEFMEEVDKEISEMKLFLNARARFVAEAYLSEFVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBB6</accession>
    <name>A0A1E5RBB6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mitochondrial resolvase Ydc2 catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2483</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBB6">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBB6"/>
    <dbReference type="InParanoid" id="A0A1E5RBB6"/>
    <dbReference type="OrthoDB" id="5552842at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000402">
        <property type="term" value="F:crossed form four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004520">
        <property type="term" value="F:DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070336">
        <property type="term" value="F:flap-structured DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000403">
        <property type="term" value="F:Y-form DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039197">
        <property type="entry name" value="Mrs1/Cce1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015242">
        <property type="entry name" value="Ydc2_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28072">
        <property type="entry name" value="CRUCIFORM CUTTING ENDONUCLEASE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28072:SF1">
        <property type="entry name" value="CRUCIFORM CUTTING ENDONUCLEASE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09159">
        <property type="entry name" value="Ydc2-catalyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mitochondrial resolvase Ydc2 catalytic" evidence="1">
        <location>
            <begin position="101"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="2">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="2">
        <location>
            <position position="434"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="2">
        <location>
            <position position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="517" mass="59179" checksum="FEB7ACA2A07DC1C2" modified="2017-01-18" version="1">MRAGSLIVEKIKFLIPEFKNNELKKLAYMLGSGNGVNKEEMVKGIVERCNLMSGMNMGEPIDMIIGKPVDMNTNNIHHTGKKKNLNTSNTLHENLYSTINVVAVDMGIANFSFSKFNVTLSKKHTSHKSTGDTNGFQINGNCINEMSAFTKKFDPLDXKHTKTVVYPQLTEWVKYDLTNKYANILNPNYPDPYYSKKSGKIKTIEKSKTIANSKYIQFNPQTVDKLCDELVKLLFPMVTSQIIKLKSSMQSLLPRTVVLLEHQRTRTGSSSNVLETVYRNSVIEYVLFGKLTRLFQNTEKHGASQIQLSDARKMCDYWVKDTKEMNKKTSKXPRLQLCYNIFDSLQNYDKPVANLKEITKKMGKIDINDCGTKTKPQLFSASPNFITKHPQLFQNNQNKNKTNKTRFYNALGMTTHDNGTMKDDDLCDSLLHGLAWIQWFEFYTNLADMVLKLEKLDVETGEMGKTRGDSVEWKKIQKLPQYQDQVTRLKNFVVTEWEKHESLLTDLTKINLLPSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBH6</accession>
    <name>A0A1E5RBH6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Sulfiredoxin</fullName>
            <ecNumber evidence="2 9">1.8.98.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2490</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>S-hydroxy-S-oxy-L-cysteinyl-[peroxiredoxin] + [protein]-dithiol + ATP = S-hydroxy-L-cysteinyl-[peroxiredoxin] + [protein]-disulfide + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17545"/>
            <dbReference type="Rhea" id="RHEA-COMP:10593"/>
            <dbReference type="Rhea" id="RHEA-COMP:10594"/>
            <dbReference type="Rhea" id="RHEA-COMP:13681"/>
            <dbReference type="Rhea" id="RHEA-COMP:17976"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:61973"/>
            <dbReference type="ChEBI" id="CHEBI:61974"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="1.8.98.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the sulfiredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.98.2" evidence="2 9"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBH6"/>
    <dbReference type="FunCoup" id="A0A1E5RBH6">
        <property type="interactions" value="361"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBH6"/>
    <dbReference type="InParanoid" id="A0A1E5RBH6"/>
    <dbReference type="OrthoDB" id="10023328at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032542">
        <property type="term" value="F:sulfiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1530.10">
        <property type="entry name" value="Conserved hypothetical protein from pyrococcus furiosus pfu- 392566-001, ParB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016692">
        <property type="entry name" value="Sulfiredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21348">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21348:SF2">
        <property type="entry name" value="SULFIREDOXIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017267">
        <property type="entry name" value="Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="ParB/Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB-like N-terminal" evidence="11">
        <location>
            <begin position="9"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Interchain" evidence="10">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR017267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR017267-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ84249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="144" mass="16311" checksum="16CE74F16DA1855C" modified="2017-01-18" version="1">MSMQSGHNKKIEYIPIRQIIRPIQPQLDQQKVDAMVSTMKGVPTASQTCTLEQASSMNGELPAVDIFKLTRRVPVANDGKTTGEPEMVTKDFYFSFASCHRLTSYERLQNETPDKEVLVKCKVYPAIESQLRLYMGASLDHWIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBN6</accession>
    <name>A0A1E5RBN6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Coatomer subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g2882</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Golgi apparatus membrane</location>
            <topology evidence="1 10">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBN6"/>
    <dbReference type="FunCoup" id="A0A1E5RBN6">
        <property type="interactions" value="1292"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBN6"/>
    <dbReference type="InParanoid" id="A0A1E5RBN6"/>
    <dbReference type="OrthoDB" id="10261470at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22948">
        <property type="entry name" value="Coatomer_WDAD_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000002">
        <property type="entry name" value="Coatomer subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000022">
        <property type="entry name" value="Coatomer subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006692">
        <property type="entry name" value="Beta-prop_COPA/B_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047312">
        <property type="entry name" value="Coatomer_alpha_WD-assoc_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016391">
        <property type="entry name" value="Coatomer_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010714">
        <property type="entry name" value="Coatomer_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050844">
        <property type="entry name" value="Coatomer_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056176">
        <property type="entry name" value="TPR_COPA_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876">
        <property type="entry name" value="COATOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876:SF1">
        <property type="entry name" value="COATOMER SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04053">
        <property type="entry name" value="B-prop_COPA_B_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06957">
        <property type="entry name" value="COPI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23953">
        <property type="entry name" value="TPR_COPA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003354">
        <property type="entry name" value="Coatomer_alpha_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <keyword evidence="4 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="47"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="89"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="131"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="204"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="248"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B second beta-propeller" evidence="13">
        <location>
            <begin position="342"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B TPR" evidence="15">
        <location>
            <begin position="627"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer alpha subunit C-terminal" evidence="14">
        <location>
            <begin position="821"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="858"/>
            <end position="888"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR003354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF06957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ84296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1216" mass="136817" checksum="27B2DD9DCE50DDAA" modified="2017-01-18" version="1">MLTKFESKSTRAKGLAFHPSRPLVLVTLFSSTIQLWDYRMGTLLHRYEDHEGPVRCVDFHPTQPLFISAGDDSIIRVWSLDTHKCLYTLTGHLDYIRTVFFHHELPWVISCSDDQTIRIWNWQNRKEIACLTGHNHFVMCAQFHPTEDLVVSASLDETVRVWDISGLRKKHSAPSHANNFEDQMQSSQNLLDGGFGDCVVKFILEGHTRGVNWASFHPTLPLIVSGGDDRQVKLWRMSSTKAWEVDTCRGHTNNVDSVIFHPFEELIISVGEDKTIRTWDLNKRTPVKQFKRENDRFWLIAAHPHINLFGVAHDSGVMVFKLDRERPPFVNHQNQLIFVNKEKQVQIFDYKKKVASLPLVSLKTIGNPWNAFRSISYNPAQHSILINTGEHDKFALCVLPKEPTGAVQPTNVIHDTGNFATFVARNRFVTYDKSTSSLEVKDLNNKKTKTIKIEGTVTNIVSASPGSILIMQPKQVVHFDVQQDKVLAQVEALNVKYVSWSHDGQYVALLGKHSITICNRDMEIVNAMHETIRIKSAAWDESNVLIFSTLNHIKYCLLNGESGIIRTLENTVYITKVHGKHVYSLNRSGEVEILTIDPTEYRFKRALINKNFPEVLQIIKTSNLVGENIIGYLQKAGFPEIALQFVQDPQTRFDLALQDFNLEIAYTECLKLKDEGIWSKLGESALLQGNADLTELVYQNKEDFDKLSFLYLSTGEQNKLSKMGKIAERRGDVNSALLNTFYSNNSVKRAELFAEHGSLPLAYAVSKANGVNVASILEEAGISESDIVLPDGYSTQSFVKSPVVNEPLQEWPLKQPELTYFEKVVLGEIEDLDIQDDEAQEIDDGTGAEEQLAYEEDAEDFHEAEDDNGQADEENAWDLGDDDLDIGDVTDGNEELGELNEKDVQAEDAISMSTETELSKWVQNSKLAAILVSSGAFDAAAQALNKQAGVYNFEPLKEKFIDLYESSKLYMMATPSELPSIEGFIRYYEEEHAKVLPFVPDITFINTKVSKGFKSFKANKLEEAIEIFRDVIYTVCLLAVDNEEDEEKAFEALKLSSQYILGLSIELTRRALPASDIKRNLELASYFTKVKLLPAHRINALQVAMTQSFKAKNFVQASHFAEELLSIVTSGPRAEQATKVKAKADSIASDAQEIDFDPYGTFDICPATYTPLYNDSPTAVDPLTGLKYKASEKGKIDSISLISKIGAPASGLRIHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDH5</accession>
    <name>A0A1E5RDH5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2333</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M24B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDH5"/>
    <dbReference type="FunCoup" id="A0A1E5RDH5">
        <property type="interactions" value="399"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDH5"/>
    <dbReference type="InParanoid" id="A0A1E5RDH5"/>
    <dbReference type="OrthoDB" id="10261878at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01087">
        <property type="entry name" value="Prolidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000002">
        <property type="entry name" value="Xaa-Pro aminopeptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007865">
        <property type="entry name" value="Aminopep_P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052433">
        <property type="entry name" value="X-Pro_dipept-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226:SF1">
        <property type="entry name" value="XAA-PRO DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05195">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01011">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminopeptidase P N-terminal" evidence="6">
        <location>
            <begin position="66"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ84941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="527" mass="59772" checksum="AB88068B85EAB526" modified="2017-01-18" version="1">MDALSVGSLLVATSALSISAAKKYMTSSNIVKDTKNAKDQFSFEPIKRNHSNGEKNPYAILETQKYPAKKHCKSTKSQLSTATITESSAIFLASNQVEGVEYSDGTKPFRQNRYFFYLTGCNLPSCYVFFNMKTEKLTLFLPPVDKDDVMWSGLPMSLKEAQDKYDVDEVLYSSELSTFFDNLESFENIYTTDTKNINDKEIAGKLKVGAPEFFYALDEARSIKDAYEVALIKKACEITDKCHLAVMSALPIETNEMHMHAEFTYHALRQGAKFQGYDPICCSGPNCSTLHYVHNDDDMEHKKTVLIDAGAEWECYTADVTRCFPIQGKFTKEHREIYEAVLDMQTHVMKNIKPGTNWDDMHFLAHKVLIDNFKKLGIFKKEFSNEEIFKRRASTCFFPHGLGHLLGLDTHDVGGEPNYSDPDPFLSYLRLRKPLAAGMVITNEPGIYFNDFLIKNFLEKHPERLEVVDVTVMEKYKYIGGVRIEDDVLVTKDGYENLTGITSDPDEIEQIVSNGIAKGRSHFHNIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDL7</accession>
    <name>A0A1E5RDL7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">CCR4-Not complex 3'-5'-exoribonuclease subunit Ccr4</fullName>
            <ecNumber evidence="6">3.1.13.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Carbon catabolite repressor protein 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Cytoplasmic deadenylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Glucose-repressible alcohol dehydrogenase transcriptional effector</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">AWRI3579_g2238</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>Exonucleolytic cleavage of poly(A) to 5'-AMP.</text>
            <dbReference type="EC" id="3.1.13.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CCR4/nocturin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="27">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.4" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDL7">
        <property type="interactions" value="487"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDL7"/>
    <dbReference type="InParanoid" id="A0A1E5RDL7"/>
    <dbReference type="OrthoDB" id="428734at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF100">
        <property type="entry name" value="POLY(A)-SPECIFIC RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="23" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="17" id="KW-0804">Transcription</keyword>
    <keyword evidence="16" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="26">
        <location>
            <begin position="406"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="25">
        <location>
            <begin position="578"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="35"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="299"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="23">
        <location>
            <begin position="172"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="24">
        <location>
            <begin position="35"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="24">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="70"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="24">
        <location>
            <begin position="88"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="24">
        <location>
            <begin position="300"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00030493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00031469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00033317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="OEJ84981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="913" mass="104126" checksum="A28813CFC5198FCA" modified="2017-01-18" version="1">MNFNNQFPQAATQQQMSYSPFNVQQQQQQQQQQQQQQQQQQQQQQQQPLGAPPGVLVGNNNVHGNGMPMHSNTLFSQQQLQNHPPGIXQQNQQSHPTQQFLNQQNQQGQQQQTQQQPQSEQSQRFMNAEMGETLSQFLGVQDASSANNLGHQHYNQVSGQGQVHSPQFQHMNQMQQQQQQQLQQQLQQQQLQHQQQLGQVPNQPLQGALLFENDPEYHPHLKNPQLLNHPNWELQMFLAMISRHMMGSESNIYAIESAINAIRGNLGGGTITNSHTEVSNRLVNATKASLIEMANSLPKSGSSVVVGNGPXDANTSVPGSSSSGSNTLSAGTPNPLTLSPESLPGTPSFEPLTPLSHNAQHGVSGMGGAAHPGSILLQHKKLSQYNIDEDDDNDVTSKQHAYDEQLWHVLDMANLKLSCISERLTSYTFLNRLYLNGNKISVIPKSIKNLKKLRVLDLSNNNISMLPAELGMLFNLKYLYLFDNKVKNFPYEFGNLISLQFLGFEGNPIDPELLKIYMDKGLTGLLFYLRDNAPEVKLVNQRQIVEIDADGEMTGNEYPSLTEAVQNQPLGPNSFSLVTYNTLCQHYATPKMYRYTPSWALSWSYRRDKLQKEILGFNTDIICLQEVETKTYEDFWLPLLSKQGYAGVFHSKTRSKTMQLKDSKKVDGCCIFYKESEFKPVFYEHQDFSTLWMTNRNFLKTEDFFNRAMSKDNVVIYLKLQHLKTNEIVWIVTTHLHWDPRLNDVKAFQTAILLDHLEKLVKEQSHLTREDQYKKEPIIICGDFNSQRHSAVYELFSKGNVQKDHIDLHGRDYGHLTKNNFAHNFQFQSAYNCVGELNFTNFTPTFTDVIDYIWYSPDVLRVRDVLGDIDSNYTSHFIGFPNADFPSDHIPMLSRFEFTKNKADNVSSRSRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDM4</accession>
    <name>A0A1E5RDM4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DnaJ homologue subfamily C member 28 conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1897</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDM4"/>
    <dbReference type="InParanoid" id="A0A1E5RDM4"/>
    <dbReference type="OrthoDB" id="1922282at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018961">
        <property type="entry name" value="DnaJ_homolog_subfam-C_membr-28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39394:SF1">
        <property type="entry name" value="DNAJ HOMOLOGUE SUBFAMILY C MEMBER 28 CONSERVED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39394">
        <property type="entry name" value="YALI0E31793P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09350">
        <property type="entry name" value="DJC28_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DnaJ homologue subfamily C member 28 conserved" evidence="1">
        <location>
            <begin position="190"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="380" mass="43636" checksum="146A1D5EE66629D3" modified="2017-01-18" version="1">MLKGKLQLLPKIFFRSYSLKKNKAESQIYQRLSDLHDQTKQDENDPLVKIQKQVAAREFQEKYAKEIAFAKVSSIANKHAQETILSQPWTGEENVKDTSLRMVLDKVGPEKNKNLHFYTNTGFKSKTLEIPKLLRTPKLSTKERLEAAQDKVLNYKLDNQEIEKKEAGAFRAXYAEKFTPIGSVEKIKSLADARIEESMRNNGFAGLEKLRGKKLDIGLNNPHVSRTEHHLNNMLVNQNIVPPWIEKQGSVNTEIANFKXSNISSFQRSLHIAFKQMKNTEVNETMKLKVFQEWKTINSVLXGSKIKSLNDSLRTYNLQAPLSTQKFYLLPERELTRIFDGVDIIKEYEAYKQERTASEKRVSNGDSSLGASIGSFLKFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDV5</accession>
    <name>A0A1E5RDV5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Hexose transporter 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2137</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDV5"/>
    <dbReference type="STRING" id="56408.A0A1E5RDV5"/>
    <dbReference type="InParanoid" id="A0A1E5RDV5"/>
    <dbReference type="OrthoDB" id="5141738at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000044">
        <property type="entry name" value="Hexose transporter Hxt3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF75">
        <property type="entry name" value="GALACTOSE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="386"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="426"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="462"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="490"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="66"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="37"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ85077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="565" mass="62176" checksum="1B68527A3D17E726" modified="2017-01-18" version="1">MSSLENKSTQSNSEAQSQLSTPPVDVQNNNKSNKAQRFNEDGTLKIDPEDEMPQKPFSAYWGVCSMCLMIAFGGMIFGYDTGSISGFVNMSDFIQRFGSHNSKGYYLSNVRTGLIVSIFNIGCAIGGLILGKLGDIYGRKIALMVVSIIYIIGIVIQIASVKAWYQYFIGRIISGLGVGGIAVLCPMLIGETAPKHLRGACVACYQLMVTCGIFLGYCINYGTKKYTGSAQWRITLGLSFLWGLFMIGGLTLVPESSRYLCEKGRIEEAKKALATCNKVEMDDPAVIAELDVILANVEAEAMAGSATWKEIFETKTKVFQRLVMGTMIQSLQQLTGDNFFFYYGTTVFQAVGLEDSFQTSIIIGVVNFFSTGVGIYTVGLFGRRSCLLVGATCMTICLCIFASVGVKALWPNGWEVGSPSKAAGDVMIVFTCLYILSFATTWAPLAYVVVSESYPLRVKSKCIAIASAANWIWGFLISFFTPFITSAIHFAYGYVFMGCTVFMVFYVFFMVPETKGLSLEEVNDLWMEGVLPWKSANWVAPDKRGADYNAEDLKHDEKKGLKRFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE41</accession>
    <name>A0A1E5RE41_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">E3 ubiquitin-protein ligase SNT2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1886</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE41">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE41"/>
    <dbReference type="InParanoid" id="A0A1E5RE41"/>
    <dbReference type="OrthoDB" id="336088at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048189">
        <property type="term" value="C:Lid2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036205">
        <property type="term" value="P:histone catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029617">
        <property type="entry name" value="Snt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47672">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SNT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47672:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SNT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00439">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BAH" evidence="7">
        <location>
            <begin position="176"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="376"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="8">
        <location>
            <begin position="1173"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="527"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="898"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="527"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="902"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="926"/>
            <end position="935"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1435" mass="163927" checksum="10DCCD625FC52D4D" modified="2017-01-18" version="1">MADGIKELQPFDADLFYTGRRSTKKVNYNEKKADAELEQNLKNSKKQKKSASPPSSFNSKKSPEPLGNNSASKKPRNESKVSKPRNVASKSSSGKKSAQNGNNSGASNKNVKKITENYVNKIVSDKSVPWNFIPSLPPAYRKHNRFSTMLPLEDAMVDVAKQILYIPGGKNYKMEILLNINEHIYMVSEPPGEPYYVGRVVAFVPKKDFYXRIDNCTGTSIFPAAYFNLKMNWYYRQRDISDSIYKSEPRILFASLHTDVCPLQSFRGKCTVMHKKXAAELFSSIXEYVTRPNHFYFDQVFDRYTGEYYSVYSTKSLLETLKPIPGSKQQSNYLKLLAMEYPYIYCEPKFPLDNFIEKYLLNHKVADSNKYDQIWDLRCGECWEWCQNTGQHIQCEGCGQPYHLYCLNPPLERKPNRDVVWVCSDCISKDNFHFEEANPKNEXLPPFKSKVPSGDLEDFAKHIINSMSLKKKTLNKDNWWFQYFGTFVVNNLESCVTFDCPYPFKTNRLGVSKYQYKFPEESCATLTSSMSNDNXTTSTPAQEKTDSGILDRGTDATVSVLWRWNESDLDEATLDKYMLDCIATFPKDRDFSPTDSNFRDFLCKALMENNFDVAKAYEQCDEQLNRKRLEIPTFTDQELSVFEREVAKYGSELKPVCKAIQTQPMAMVVKFFYLWKKTERGLKVRNKWDKKLKAANLKKXPNLSKEWKYIDDSSVELDNLPKETEISCSFCLTDFSPMWYKLTGCSTKPAKKENSETFRLQSLCIRCARLWRRYGVKWEPPSNTLKAVYGGNASSFKANVAELLSDNSESQHNVKVTASLAYQKKIEWELILDSELIIKQRESIFLDNSRASKYKKNISNLRSTMSKSLMQLVDNSLTNNDFMGKKLEKYLAKFEKXKSEPKSASPQTXVANAKQTPXSPKAHASSLEQSXTPDTKNAKTPEVSXNSVSKPTKRKLPKVLSSDGLGHTTGAPNTKSTGLKVNCTLPNNENCELLIDPDFKTVRIANVSDLFKNYTERIAKWDLPEIPDTLKVETSLDELNLQFSAASTENNIPVLLNESKEKVLDVLHQHALKVLEKAHVSVPHTKKIRSKKGGYRDSMALKSTTNSNKLRDCCSVCMGHMSPEELQAEEIICNNCGMNCHYYCYGKSKDLVGSNWLCXPCSNDVQTIASQDYRCCLCVSKEIDYEACRKLLSSAIPDALKRTDNNKWAHVICALYQPGVSVEHPSLQPLQHIKHVCRSLKCSICSNTSGSVIKCDLCENRFHVTCAQDEETFQLGFTSEEALQPVLLCSLHSDSRLKPLDFKLADGKTLWRKYLESVIHRNITSSDVTGLKSEETRQCPKCGTKESIYWYAENVCRGCYIDELYPGARPKILAPVFPNDKKDEEKDHKENKNKFLENIDPSKFQTQFMRNKFKANNKRLMVEESEQKNSKKIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REA3</accession>
    <name>A0A1E5REA3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NADH kinase POS5, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1928</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REA3"/>
    <dbReference type="FunCoup" id="A0A1E5REA3">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REA3"/>
    <dbReference type="InParanoid" id="A0A1E5REA3"/>
    <dbReference type="OrthoDB" id="24581at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.30:FF:000014">
        <property type="entry name" value="Mitochondrial NADH kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF26">
        <property type="entry name" value="NADH KINASE POS5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ85231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="442" mass="48998" checksum="47409D0C61E8DC4C" modified="2017-01-18" version="1">MFWNKLLFSSANTGPTAIRFYRGLNTASTCISHVNVKPVSSLKGRTFPDYVNSPNSKLQSLIWPKPIQNIFIVKKPWSEKTTAATAELIHHLHAKYPSINVIVQDNIIDELKPRLLLTPENSSCKIDETHDTIPLFTGTEEEIVKKTDLLITLGGDGTILRAISFFNTDYVPPVLSFSLGTLGFLLPFEFKEYSKVVDEIIHSRSKVLYRTRLECYITKRSKNQIKTIQEKSDDGKDAPKQITKVHAMNDIFLHRGDSPHLTYLDIYIDGDFLTRTTADGVVLSTPTGSTAYSLSAGGSIVSPRVPCIMLTPICPRSLSFRPLVLPHSSHIKIKIGSKSKKKVAGGEPQAKPIKLNIDGVSLNDLEYDDEIHIVNEVGTIYLDSNSNIPYSKTGVPPLDAGYKGLSNNTKLAGIHCLARSENDWTAGINELLGFNSSFKLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REL0</accession>
    <name>A0A1E5REL0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetylglucosaminylphosphatidylinositol deacetylase</fullName>
            <ecNumber evidence="2">3.5.1.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1674</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PIGL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.89" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REL0">
        <property type="interactions" value="540"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REL0"/>
    <dbReference type="InParanoid" id="A0A1E5REL0"/>
    <dbReference type="OrthoDB" id="3972725at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000225">
        <property type="term" value="F:N-acetylglucosaminylphosphatidylinositol deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10320">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003737">
        <property type="entry name" value="GlcNAc_PI_deacetylase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024078">
        <property type="entry name" value="LmbE-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993:SF11">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02585">
        <property type="entry name" value="PIG-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102588">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="299" mass="34821" checksum="7EBC0156DF09A3CB" modified="2017-01-18" version="1">MKRGLYAAGFMGLLLSALFSSLLLLQPSNTRSTIKKINDRSLELLLXKDLQSAXYTSVNLVIAHPDDEIMFFSPLLNVLAXMETNMKIRVVCLSNGDXDGLGYIRELELQXSVKAIFHKISNFESYSSXESPPPPPRDFEKNQKTLEIHIGGMIDGMDQSWDLXEIYNKYLMPVMTDGNPLFITFDHXGISSHINHITCSKVVQKYYENVLVLQTPNLLQKYSSFMLLPYHMLKAWYSXTVHPSXSNEQKNFLFVNTWYDYKASLQKMRITHRSQMVWYRYFWWVLSSLVFYNNYEYIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REL6</accession>
    <name>A0A1E5REL6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Homocysteine S-methyltransferase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1670</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REL6">
        <property type="interactions" value="219"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REL6"/>
    <dbReference type="InParanoid" id="A0A1E5REL6"/>
    <dbReference type="OrthoDB" id="261426at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.330">
        <property type="entry name" value="Homocysteine-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003726">
        <property type="entry name" value="HCY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036589">
        <property type="entry name" value="HCY_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11103:SF10">
        <property type="entry name" value="HOMOCYSTEINE S-METHYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11103">
        <property type="entry name" value="SLR1189 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02574">
        <property type="entry name" value="S-methyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82282">
        <property type="entry name" value="Homocysteine S-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50970">
        <property type="entry name" value="HCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Hcy-binding" evidence="4">
        <location>
            <begin position="6"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="356" mass="40278" checksum="3DB25C7D25F7591A" modified="2017-01-18" version="1">MRKSLADQLKDPNDKTVFVLDGGQGXELENRGINVSNPVWSTVPFLDSSFWHSHNGLPNGCSKHTQRENEKTQDRQIVEGMYHDFMDEGGCDVCMTITYQSSFTSLKQHTDIKTLKEYQDLMEKIVLFTREVIGEEKYLIGSIGCYGAHICSEFNGDYGSEPEKIDYYEYFKPQLDVFCFSEKLNKCNVIDMIGFETVPNLHEIKAILSWGTDKINKPFYISLSVGNDGKLRDGTDLADICTLLKQLISDKKLNPNFTMLGVNCVSXKQSLSMVANLHNNLQGLDVPLIVYPNSGEEYDPEEKIWLTRDQGDIASFSWDKYVPQFVKNNCRIIGGCCRTTPADIAKIRKATDLCTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REU8</accession>
    <name>A0A1E5REU8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial distribution and morphology protein 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Mitochondrial inheritance component MDM12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MDM12</name>
        <name evidence="12" type="ORF">AWRI3579_g1829</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the ERMES/MDM complex, which serves as a molecular tether to connect the endoplasmic reticulum (ER) and mitochondria. Components of this complex are involved in the control of mitochondrial shape and protein biogenesis, and function in nonvesicular lipid trafficking between the ER and mitochondria. MDM12 is required for the interaction of the ER-resident membrane protein MMM1 and the outer mitochondrial membrane-resident beta-barrel protein MDM10. The MDM12-MMM1 subcomplex functions in the major beta-barrel assembly pathway that is responsible for biogenesis of all mitochondrial outer membrane beta-barrel proteins, and acts in a late step after the SAM complex. The MDM10-MDM12-MMM1 subcomplex further acts in the TOM40-specific pathway after the action of the MDM12-MMM1 complex. Essential for establishing and maintaining the structure of mitochondria and maintenance of mtDNA nucleoids.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the ER-mitochondria encounter structure (ERMES) or MDM complex, composed of MMM1, MDM10, MDM12 and MDM34. A MMM1 homodimer associates with one molecule of MDM12 on each side in a pairwise head-to-tail manner, and the SMP-LTD domains of MMM1 and MDM12 generate a continuous hydrophobic tunnel for phospholipid trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Mitochondrion outer membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="9">The ERMES/MDM complex localizes to a few discrete foci (around 10 per single cell), that represent mitochondria-endoplasmic reticulum junctions. These foci are often found next to mtDNA nucleoids.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the MDM12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REU8">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REU8"/>
    <dbReference type="InParanoid" id="A0A1E5REU8"/>
    <dbReference type="OrthoDB" id="3356905at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21672">
        <property type="entry name" value="SMP_Mdm12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03104">
        <property type="entry name" value="Mdm12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027532">
        <property type="entry name" value="Mdm12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26544">
        <property type="entry name" value="Mdm12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 9" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SMP-LTD" evidence="11">
        <location>
            <begin position="1"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="65"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="87"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ85404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="301" mass="33959" checksum="BA16E3CBEE9AEB9B" modified="2017-01-18" version="1">MSFDIDWDKIRNDGSINQSIQDFLNDXLSSTELPKYIKNLKVLNFDIGDEPPLIELKQISDPLPAFYESSDEEPDEELELGNEEATSKSNEQKTNNVNNTDCEERIKNKDKMDTQLLVDIAYKGNMLLEISADISMNYPTDSFVSLPVKLLISDIGFHSLCIIAYLKSKQESKNAVDESSIENRIFLSLLCDVADSELDXPKSAQNQDPQSPNLSNQQAGLPSISKATNYRQQQYHFPSSMERMQFLRTMTVKTEIGGDGGSSSNASALKNVGELETFLLEMLRGLIRKELCWPGWIEFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF87</accession>
    <name>A0A1E5RF87_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ISWI chromatin-remodeling complex ATPase ISW2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g2099</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RF87">
        <property type="interactions" value="1211"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF87"/>
    <dbReference type="InParanoid" id="A0A1E5RF87"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17997">
        <property type="entry name" value="DEXHc_SMARCA1_SMARCA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000082">
        <property type="entry name" value="ISWI chromatin remodeling complex ATPase ISW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000015">
        <property type="entry name" value="lymphoid-specific helicase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.30">
        <property type="entry name" value="ISWI, HAND domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044754">
        <property type="entry name" value="Isw1/2_DEXHc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015194">
        <property type="entry name" value="ISWI_HAND-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036306">
        <property type="entry name" value="ISWI_HAND-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015195">
        <property type="entry name" value="SLIDE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF49">
        <property type="entry name" value="SWI_SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY A MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09110">
        <property type="entry name" value="HAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09111">
        <property type="entry name" value="SLIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101224">
        <property type="entry name" value="HAND domain of the nucleosome remodeling ATPase ISWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="266"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="579"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="12">
        <location>
            <begin position="1004"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="181"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="856"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="918"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1182"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="101"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="857"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="939"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="956"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1187"/>
            <end position="1202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ85555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1223" mass="141383" checksum="70EE7A68E413EC6D" modified="2017-01-18" version="1">MGQPSRKRSTRPTTRTVIEVDSGDSSSTEGEYESTQEHNSNSVSESDEEPIKNDSPLKKHATKVRIADPFSEEEDDAASSTTKTDIPEQKYQGDEDAQENSVHKDNSHEAESDGVEEYDADYDTHGALYWEERIKRFFDTKATNKQDSYDRYKYLLNLNGLFRNFISQKSKKDKQVQRLLKDYDKKEMTSSKSAESSGRHRKSEKEEDQEMMLLEEDGEDYGYDDSELDLVDIQDEVDTGFFKKSPNYIKEGKLRDYQIQGLNWLVALHKNKLNGILADEMGLGKTLQTISFLGYLRHVAKIDGPFLVIAPKSTLDNWRREFAKWTPDVKAIVLQGDKEERKNLIKNKINKARFDVLIASFEIVLKEKNFLKKFAWQYLIIDEAHRIKNEDSMLSQIIRLFYSKNRLLITGTPLQNNLHELWALLNFILPDVFESAALFDEWFGNNKEEAIEPGSDKGSGDNKEKDQDMIVKQLRTVLSPFLLRRIKADVEKSLLPKIETNVYVGMTDMQLKWYKSLLEKDIQAVNGAVSKTEGKTRLLNIVMQLRKCCNHPYLFDGAEPGPPYTTDEHLVYNAGKMIALDKLLKKLKAQGSRVLIFSQMSRLLDIIEDYCFLREYECCRIDGSTAHEDRIAAIDDYNAPNSNKFIFLLTTRAGGLGINLVTADTVVLFDSDWNPQADLQAMDRAHRIGQKKQVRVYRFVTENAIEEKVLERAAQKLRLDQLVIQQGRGKSNMNDQKIGSSKDELLSMIQYGAQDVFDEKSHKISVDDDIEEILKKGQLKTQQLNQKYEKLGLDDLQNFNAFADQSAYEWNGTNFQKKSTSDNLGEQWINPGTRERKRRHEVQSYSVDDYYREKLNSSKNASSVNNTVDHIKQPKTRNMPDFQFFPPQLKFLDEKEILDWKRKVGYKVTKFDIKLKEESENAASSTNKAKKXSRSKNGESSSDFSDASSSSDEEEHTSADSEEKKEDGKELTMKEKIALEQQIIDNAPIFTEEDAQQQEKLASEAFTSWNKREMMQLINLMATYGKDKYDIFAKNLENKTQEEIENYCKVFWQRYQEIDGYQRYLRIIESNRKKAERLEHQEYVLKRKVANCKSPLHDLIIQYPQHARKPYSTLEDXFILWRLSHYGLFHENVYEKIRQDILMSNVFPFNWYLKTKTAQEISKRVTTLLALVSKEMDALDKKKRKAEVKDESPDMNGKKQKIDGIINDVPADAVSSVSPTPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFJ2</accession>
    <name>A0A1E5RFJ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Signal peptidase subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1949</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Essential component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Essential for the SPC catalytic activity, possibly by stabilizing and positioning the active center of the complex close to the lumenal surface. Essential for viability.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SPCS3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFJ2"/>
    <dbReference type="FunCoup" id="A0A1E5RFJ2">
        <property type="interactions" value="429"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFJ2"/>
    <dbReference type="InParanoid" id="A0A1E5RFJ2"/>
    <dbReference type="OrthoDB" id="10261524at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007653">
        <property type="entry name" value="SPC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12804">
        <property type="entry name" value="MICROSOMAL SIGNAL PEPTIDASE 23 KD SUBUNIT SPC22/23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12804:SF0">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04573">
        <property type="entry name" value="SPC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016089">
        <property type="entry name" value="SPC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR016089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="198" mass="22584" checksum="5CD57A3794F1A1EE" modified="2017-01-18" version="1">MFSLNQRFQSVTNTALTYCVLLIALVIALTQLQLSVHNKVYDSIPLSNFHINNIQTNIRSSRMFGGSNKQNMKLKFDLSTDLSSLWTWNTKQVFVYLTGEYQDSTGSKIGKSTGKVTFWDKIITQKEDAVLDMENIVSKYSVWDKNSQLKDKIINVKLQWNIQPYVGPLNFGEIVVPKELLPNTVFTVPDVKRKSVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFP7</accession>
    <name>A0A1E5RFP7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2198</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RFP7"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ85704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="152" mass="16825" checksum="DC355074317A7BC2" modified="2017-01-18" version="1">MATDITNYYANLDCGTFNISAXAYSNDSFTIDQFNEYYYCGLSYGSYMYMGQNFTHDDLLQNSTHQGWEDRGNTYAVFDCGGGYTKNVSTIFDGATGNIFPHVDAYDCGQTFFSSKATKTSTTHAKENAGFKMDNNKSLFVMGMIAVAALFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGC1</accession>
    <name>A0A1E5RGC1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Epsin-4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2316</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGC1"/>
    <dbReference type="FunCoup" id="A0A1E5RGC1">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGC1"/>
    <dbReference type="InParanoid" id="A0A1E5RGC1"/>
    <dbReference type="OrthoDB" id="4033880at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="2">
        <location>
            <begin position="10"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="299"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="345"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="367"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="393" mass="43992" checksum="57AEB3CCB9B133C1" modified="2017-01-18" version="1">MFKSFFQFSSSTEQKIKNFIDDTKGTKKSNTLMQEIAVLTFSPKTLKEIVAVFRKRLQLIANIMLQYQELIGKLEKNKRSGNRGDFNGNKSGRGGSVCGVNTQLTHQSLQVLNALTLMEYLLTNGSDDFVSWTKRNLVYYIAPLMKINFPGASTTGANLDNNPSLSLLLLLQKHSHKLQKMISDTQSISKQREEIVTFRNSISFAGRTSTDNSPLKKKFQYDGTTRSASLDFSSRYSASPTVGKKGELGKLPKRSQTLYTNYKQRGRNNGDDCQNNNPFLQRNDYNTGTNNKSITATVNTFNDINDSSNNNNTTNDYNNNSRNYARRSLDLHSHGKTLGPIKTHGLQSLNTQGNNSLQFQPYHINNSPTTSYRSYTSSPLSAGTGHLDPVVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGM1</accession>
    <name>A0A1E5RGM1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Histone transcription regulator 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1157</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HIR3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGM1">
        <property type="interactions" value="168"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGM1"/>
    <dbReference type="InParanoid" id="A0A1E5RGM1"/>
    <dbReference type="OrthoDB" id="77564at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000417">
        <property type="term" value="C:HIR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033053">
        <property type="entry name" value="Hir3/CABIN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15502">
        <property type="entry name" value="CALCINEURIN-BINDING PROTEIN CABIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15502:SF7">
        <property type="entry name" value="CALCINEURIN-BINDING PROTEIN CABIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="335"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="335"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="360"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ86027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1605" mass="186598" checksum="8873A417FD694AA6" modified="2017-01-18" version="1">MSSFTAVNLEHDDDFQEQEEHTRELQVEQGYSLYNAGLSLYKDKKLEEASQKFDQLLAIEILKXNRFGNFSYNSPAADRLRYLAYRNRGFLYVDYLHQNYESLDNHTIVDDLLKAVEYLLEALSHGHADYKCTDLLLTLLKAFNSKKLQRWVLELELAKDDFILNSSTLLPQWRNLISSEQQLVSDLNDLEHTSTFLKSLFSLNNYQQLSDKEASPLMVKIQKMKREDENLFRELDFDEIILEEKTWEHLTQRLETLLPSRKTAFNYSKXSDPYTDLDDPIESIVLKFSASTEKKTXPFSSQDDILSKSLTXLQTDSKKDSELLSFFAQQELKDSQNDESLHSKRLAEERPQRVSKRVRDKTTDTETDTKHSDIQQLFLKEFKMLLDAVGFSFASAEEVESIKFLKDTSSESLVSSDMYDCISSWSNKYTEILKASEANEREDSQGLTDFVNILKASLNLGDNTMSVKPLCDEHLGSFVESVNSRKMHFHQVRLQLLFTLTSVGDKGECFITDRLWTGDLFKMIEYSVLTLEQHILPILNISKLYDVSIAVSIYEVLVNFFCKLSKELTLKRLQNQRVSDIENLMSKIMARINEWKMLFFRKVERTKFVKKIFLRHDWASLYLTQSIHMADQESFLAELQKFKQNLSQDDIVSNCNYGELPSISKTDIDNLVLKYEIIYSFDSKDVAQEHKQVESLIKVLSNEKNSYTPQELTLQKFLLGCPIQVKLQIWTVIFNHLSKFGNSDIMERCFFTMLEYIDVFFNSPSYNTMEHLQRHQLFLSVIGYLTNLTKVYVDYLCKCDQLMPTVSSSKMDLLLRYFRLFYSIINYEIMCIKDSQLQSFFKKAANSSQKLKTLLCCWSILIAVHYQQLLKRIPQWEQYVERLLNNFHALFAFFKFCDVADNAFLKYSEKILCGFDSSSVLPQIQQILYCRYHLQLSSGERSEVELHDTKKTDIDEESAIQIGSYLIRNQYGASNPMLFSGTKYSMKQIFDVIVDAIGTPNYETCFLAKRNECYFEEFLELPVKIMYFKNALNGSMGLDLVDTESPYEKVIKSKIVLVAAIQQFNIFKSRKRSNLARLSELDITRSLLKADIVYNSGRFESWYLLGKCYSYMAEDDMTWTSDKLIALDKKKITAAYQRKAIICHLMAINTFNKIARVDSNMKNEDEAILEAIFESLGEVLMTAWFQPMKGLCFSQDLDHILCMDPHGELYETGASSAQTISEHNVKQLMLCCFDIANMHVAKDQVMDSSSYWMVPFYKAKVNYKADKHFDDYKSCALDACFAAINDKSSKTSFIMEPHYFIVSNCYKLVRNGALTVSEALTFLKQDNKLFELEESFWVCEGITVKSFNEKLVQMLEILIGLDKKKWYHRPKYRIATIYAYSLANIDKAVEFMEELVFAASPNKSLVTIWKPDHELPGKHFVYITQYIRFYLELLFEKKDLAKIGQVSKKLRKFGSNMLKSNEMQDYATKMYAELVKMLFRFDEKEIVESFLPQVSNTIFTRTSSKILETCLSLNSNEEAINVFLETNDQRKGSYTQYDDLCIALYFKFLFLPTVENDPDYISTKETVDLDSFVKGKNVQNQVKKRVSKKEVLEQVSQLCKKIKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGM7</accession>
    <name>A0A1E5RGM7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Exopolyphosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1158</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGM7">
        <property type="interactions" value="244"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGM7"/>
    <dbReference type="InParanoid" id="A0A1E5RGM7"/>
    <dbReference type="OrthoDB" id="374045at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004309">
        <property type="term" value="F:exopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.20">
        <property type="entry name" value="DHHA2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1640.10">
        <property type="entry name" value="inorganic pyrophosphatase (n-terminal core)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004097">
        <property type="entry name" value="DHHA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038222">
        <property type="entry name" value="DHHA2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112">
        <property type="entry name" value="BNIP - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112:SF39">
        <property type="entry name" value="EG:152A3.5 PROTEIN (FBGN0003116_PN PROTEIN)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02833">
        <property type="entry name" value="DHHA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01131">
        <property type="entry name" value="DHHA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHHA2" evidence="5">
        <location>
            <begin position="230"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ86034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="375" mass="42547" checksum="CB2BEB19D5F48F6D" modified="2017-01-18" version="1">MTTSLAGFLGFLKSSYIREQLARGDVLNIVCGNQSCDMDSVMSALTYAYYSYLKRPEQPIIPVLNIPKEDLKLRKDIIYVLSKKANISQGDNLLFFIKDIXNYKQEKKLNIHAVLVDHNKLEGEAELVVDEIVGIIDHHEDSGTSRDSIIKYHGPDIIKVTGSCSSLITNYWARQLNNDLSAMKNTVELSLAAVLIDTNNFQYKVEAPDEEAFRIYKSQLSPTYSLEKYFDEIKKEKNNLDGLSISDLLRKDYKQFEFGGYRVGMSAIVKPTSWLIEQYGADEIVHDCNEYSREMXVLILLTSFMEKGNFHRELVIVTKNNQLQSQILKGIESPLKLEALQETGFGYTVYNQKNTESSRKQVAPAVKNAIEKTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGU6</accession>
    <name>A0A1E5RGU6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">OTU domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1317</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGU6"/>
    <dbReference type="FunCoup" id="A0A1E5RGU6">
        <property type="interactions" value="695"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGU6"/>
    <dbReference type="InParanoid" id="A0A1E5RGU6"/>
    <dbReference type="OrthoDB" id="415023at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22762">
        <property type="entry name" value="OTU_fungi_OTU2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049771">
        <property type="entry name" value="OTU2-like_OTU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050704">
        <property type="entry name" value="Peptidase_C85-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419:SF10">
        <property type="entry name" value="DEUBIQUITINASE OTUD6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419">
        <property type="entry name" value="OTU DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OTU" evidence="2">
        <location>
            <begin position="180"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="115"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="346" mass="40116" checksum="70521F565DDDDF00" modified="2017-01-18" version="1">METEKSDIESKECLLARHKKEKKDLQNQVTGMKKQSGGNKSKRKQINSKCAELEESLQKRHEQEVREWEKFHGLGETSIDDDNEDDDEFSPEKLLKELEIRQKEEEPNETETSNASKQDSSSAQQAQPKKRRNRQREKLAKREAEIAKIKEEAAKEAMEQPDLKKMEQDTLDKIFAMNNVRPYDIQPDGHCLFASILDQLQTRHVGKKNTEIMEHYNIPNDVAAQVKQKPIEQWNVADLRSLAANHVREHKDNFIPYLFDEATMSLLDINDYTEEIEKTAKWGGDVELIALANVFNCCISIIFSGRSTLKINDLENEQTPRNPELKLVYYKHTYALGEHYNSLRDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH64</accession>
    <name>A0A1E5RH64_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1203</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH64"/>
    <dbReference type="InParanoid" id="A0A1E5RH64"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="289"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="104"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="232"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="297"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="328"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="365"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="3">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="375" mass="43052" checksum="7B87FDDC0DC69CDD" modified="2017-01-18" version="1">MSNITPSEMPTEQETIDEIVRWKAKTTINFDPIKKYINFNNSIERLVDALLPKNNGDSTEDKASEFKQTGKPFTSANYVPSQILKHEMEFKILSLEVDQYLRMLENLVQNHQDDELENDATQDEAQEQQAQQDKEQYEFFQGFLKVIETKNTAIQKFNKSQIDNMLPNKSYKDVSYYKILKKEYQKKIQKHELERSHDLGIMRKNQDFVNTLEQLLATHERVLSSIDKEVNTDEEEAEEEDVDMSLSDSDLDIEALVASNALDDDFSSEQQEHNELLEEDEHDIEELIHENDDENIKDESMQERDGKTDLRQAIEVEPEETAAVSASPPGVEETPGVEETPGVEETPXXEEIPGVEEIPGVEETPGVEETPEETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHA1</accession>
    <name>A0A1E5RHA1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Clathrin coat assembly protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1079</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHA1">
        <property type="interactions" value="424"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHA1"/>
    <dbReference type="InParanoid" id="A0A1E5RHA1"/>
    <dbReference type="OrthoDB" id="44015at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16988">
        <property type="entry name" value="ANTH_N_YAP180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF5">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-BINDING CLATHRIN ASSEMBLY PROTEIN LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="2">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="420"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="501"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="546"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="640"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="277"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="431"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="502"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="519"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="546"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="590"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="640"/>
            <end position="664"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="677" mass="77470" checksum="701A99EE9CB37AEC" modified="2017-01-18" version="1">MTXYEKLVKGATKIKMAPPKPKYIEPILLGTTNPDNFHEIVSCLDRRLHDXAFTISYKSLITLHLMLNEGEKTITVKYLNKHLEVFENLRVKASHMDSVALENYNEYLKVRCEEYKPNLMGKDFIREGETFVIKYFQTYNNNNFGKTEQNAEKLLIIISSLERIIVQLLKNKYSQFDLSNNDLLMTCFKLLIKDLLILYQALNESIITILEGFFELNHELARKTLKLYKRFVNLTDEVVKYLKMGKSCGLKIPIIKHITTKLVNSLEDHLQSEELTSKNGNAGNTTNTNTNTTTTSTSTAALNSNVPPNNNNNNNNKNGLSPREQLERIRRQKEVLEQQLKQTSTLIPQTPQMNQSPSMMSNNGASALTSPVQPQHTNNPFLMDIQQSTGQQFATGQQQTMTGQFQQPAMTGQFQQPAMNGQVQQPAMTGQFQQQQQQQQQQQQQLHPQFTQHHMQHQPTMPQIQENGLMNTQTGFNGTINNMASSNGLGLLQQAHTFSNLQMQQPQQSQEFQAPKQNVRAQTTGSNNPFSMANVQEQNIKRQDTNPFSLQNYGTEGATQQHQTQSNPFSIGNTPAAQLPVFTNTTGLNQPAQQTQSFQQSQQPQLQQQYTQPQLQQQYTQPQLQQQYTQPQLQQQYTQPQLQQQYTHPQLQQQQQQQYTQPYTNTNMNEHPSLIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHE2</accession>
    <name>A0A1E5RHE2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Endoplasmic reticulum transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1261</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BCAP29/BCAP31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHE2"/>
    <dbReference type="FunCoup" id="A0A1E5RHE2">
        <property type="interactions" value="638"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHE2"/>
    <dbReference type="InParanoid" id="A0A1E5RHE2"/>
    <dbReference type="OrthoDB" id="435607at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070973">
        <property type="term" value="P:protein localization to endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008417">
        <property type="entry name" value="BAP29/BAP31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040463">
        <property type="entry name" value="BAP29/BAP31_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701">
        <property type="entry name" value="BCR-ASSOCIATED PROTEIN, BAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701:SF20">
        <property type="entry name" value="ENDOPLASMIC RETICULUM TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05529">
        <property type="entry name" value="Bap31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="BAP29/BAP31 transmembrane" evidence="6">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="216" mass="24839" checksum="26ED923220AF6F5A" modified="2017-01-18" version="1">MSLYFAFIYGLLVIEMGIFSIMILPMPNFLMGSKKHFIRAVNFVIQGNDTLKMVSKCVIIFITIFFLDCVNKLNKLQMAIQVFSTSKISMGYNTPMNQNINNNNNNNMAGLVDGMSNEYMNLSKNELYKNKFYSQRNLYLTGFTLFLSLAILRISSIIMDLLNVKDNLNKHLMVKDSIINKENLQKTQLENNEKVKSDIAEMDKLIKELKERGIST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHF9</accession>
    <name>A0A1E5RHF9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Myosin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AWRI3579_g1464</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Actin patch</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHF9">
        <property type="interactions" value="367"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHF9"/>
    <dbReference type="InParanoid" id="A0A1E5RHF9"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007121">
        <property type="term" value="P:bipolar cellular bud site selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000601">
        <property type="term" value="P:positive regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006970">
        <property type="term" value="P:response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01378">
        <property type="entry name" value="MYSc_Myo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11858">
        <property type="entry name" value="SH3_Myosin-I_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000015">
        <property type="entry name" value="Myosin I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000004">
        <property type="entry name" value="Myosin IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000007">
        <property type="entry name" value="Myosin IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035535">
        <property type="entry name" value="Fungal_myosin-I_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036072">
        <property type="entry name" value="MYSc_Myo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF837">
        <property type="entry name" value="MYOSIN-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06017">
        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 15" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 15" id="KW-0505">Motor protein</keyword>
    <keyword evidence="10 15" id="KW-0518">Myosin</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 14" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Myosin motor" evidence="18">
        <location>
            <begin position="37"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="domain" description="TH1" evidence="19">
        <location>
            <begin position="771"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="1117"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="15">
        <location>
            <begin position="587"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="953"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="980"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1031"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1075"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="1104"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="130"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OEJ86317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1252" mass="139836" checksum="A51E9ACB6E46C2A1" modified="2017-01-18" version="1">MAIIKRGARNKNQAAPEKRSAKKIQKAAFDTTKKKEVGVSDLTLLTKISNESINENLEKRFKNGIIYTYIGNVLISVNPFRDLGIYTDAVLQSYKGKNRLEVPPHVFAIAESMYYNLKAYNENQSVIISGESGAGKTEAAKRIMQYIAAASSSQQHSITKIKDMVLATNPLLESFGCAKTLRNNNSSRHGKYLEIKFNATFDPISANITNYLLEKQRVVSQIKNERNFHIFYQFTKGASDNYRQIYGVQKPEQYVYTATCTTADTIDDLKDWQATLQAMNTIGVTQQEQDEIFRMMAAILWIGNVTFVENDEGNSQVKDTSVTDFVAYLLQVDNQILTKCLVERIMETNHGMKRGSVYHVPLNKVQATAVRDALAKAIYNNLFDWIVSRVNVSLGALSGGDKSIGILDIYGFEIFEKNSFEQLCINYVNEKLQQIFIQLTLKSEQEEYAREKIQWTPIQYFDNKVVCDLIEARRPPGIFAAMNDAVATAHADSSAADQTFXQRLNLFTSNKHFDLRQNKFVIKHYAGDVIYDVLGISDKNKDQLQKDLVELISTTSNAFLKTIFPDTVNTESKRRPPTAGDKIITSANALVETLSKTTPSYIRTIKPNQTKRPEDYDEKQVLHQIKYLGLQENVRIRRSGFAYRQTFEKFVERFYLLSPKCSYAGDYIWQGDEIEACNQILKDARIPISEYQVGVTKVFIKTPETLFALESMRDKYWHNMAARIQRAWRRFLQRRIDAAASIQRAIREKKSGNKYVQLRDYGTDKVLAYRKERRSMSLLGYRAFMGDYLSCNEPKSKGSFIKKQAGISEFVVFSMKGEALLSKFGRSAQRLPKTIILTKSVLYVVGQTVLNNQISYTVDHKIPLTQIGNVSLTNLQDDWIGVYVNNAAQPDPLINCIFKTELITHMKKLNPKLQVKIGPNIEYQKKPGKVHLVKSKVDASAPLNSDFYKSSTISVRKGNPANSRSKKRPMKKASSTFNGSTTVRTSVKTTTVTSPKKTLDPFSSQVNGAQKPAAPVPARKSKNPAPPPPSSSQRNGTNASAMAAAAAQSVYHPQQQNQPAARPQPQARPQPQARPQPQAQPRAEPTPQTAAQSSVVNNGAVNKPGPPPPPPPPPAQKQDPKYKAAYAFVGTGSPSEMPLALGQVVFISRHEPSGWSLAKSLDGSKEGWVPSAYIVEITESASVPSSQPLQQSAPQVEQVQQVQQVQQVQVQQDAAPAGADFSAGLANALAARANKVRQDESEDEANSDDDDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHG1</accession>
    <name>A0A1E5RHG1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">SWI5-dependent HO expression protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1467</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SHE2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHG1"/>
    <dbReference type="FunCoup" id="A0A1E5RHG1">
        <property type="interactions" value="112"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHG1"/>
    <dbReference type="InParanoid" id="A0A1E5RHG1"/>
    <dbReference type="OrthoDB" id="4041888at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.20">
        <property type="entry name" value="She2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024261">
        <property type="entry name" value="RNA-bd_She2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036827">
        <property type="entry name" value="She2_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11435">
        <property type="entry name" value="She2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116942">
        <property type="entry name" value="RNA-binding protein She2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RNA binding protein She2" evidence="9">
        <location>
            <begin position="17"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="243"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="248"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="261"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF11435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="289" mass="33338" checksum="9A23756683A54D90" modified="2017-01-18" version="1">MSSINFVLDESLVESLQEMITAHSCYLSEYINLLNRYINFLKKVSSLKNERAGLIKFVKKLRYLNNYYTFNEKIVILAEDEQLEDIPLIPIIRRVCSYNIKVLEILDILNFQVTQSIKNEILSKTLNYNIVFDDQFVKIMEDTFRVFIKFNQWMFESLEITGCDMNALDSSLSIELIDVATKCAIDDGIDLEQSNDIILQQVETCSTEEEYLTTMNEWIDIIKQNLQKFNTNADTMVAAFHNKQGSQNHGTNNNNNTNGTVLSNKGSASPFSPTRSNFLTTPKKNMRRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHI0</accession>
    <name>A0A1E5RHI0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1542</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHI0"/>
    <dbReference type="FunCoup" id="A0A1E5RHI0">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHI0"/>
    <dbReference type="OrthoDB" id="4064185at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ86337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="643" mass="74629" checksum="C666E42CB11D1346" modified="2017-01-18" version="1">MASNLAFFHNTLHAVPAPSTIKDALPSHILFQLSNDLLEKVNCTLQSEILNLLNSNKELNFIVANSYNKDSKLRQLIRTSMIMDPNSLQLCNSVLMNPEMKSLSIFSAYTHTVFENKTQLFHWIIHDCTQHQDFFELTTLVLKMWNKNPESLENEIVFKKIILPNFLLKQKDEINTRDLRFLLSFFEKNPHITLNVSEISSFLTKANSVRKINPELNKKCLGFIFNLNHKENFNQSLVILDHYDSLLQDIAISNVAGVAMTWEALLKVQPDLLKHNTYIIYHLLKTFKKTILYQDYGLKIFEKLYPVEYFLKHQSLIGPFLQYAILKKDSDMAQNIITTISENVENYSKFNLSKYYSQLLILHLSFNDSNGTSNMISKILKRQKNNLLPPYDLELIIRHILKFPRSKANESDQTQKMKVLSVKIQEAIYLFNKTTKDLSNQELVAYYTNCYPPVIKACVENLKLCRNSAYEITNQTQYLKNINIIDAMFKNVKKWTPPGQKPPRAFQQSISSIYFDFLTHCTLQPKALSAVNCLQIAKTNFTMSLNELANKTVSPQNPFNINQNLAITTKLIVNYANRIVILKNILKNAKKQKNFDITKWCVGELKKYMGQDSYAFEQKILSQKYHQRQSKNEKNHVNIMSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHL4</accession>
    <name>A0A1E5RHL4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Zuotin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1016</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHL4"/>
    <dbReference type="FunCoup" id="A0A1E5RHL4">
        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHL4"/>
    <dbReference type="InParanoid" id="A0A1E5RHL4"/>
    <dbReference type="OrthoDB" id="1690618at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051083">
        <property type="term" value="P:'de novo' cotranslational protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23953">
        <property type="entry name" value="zuotin_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.840">
        <property type="entry name" value="Ribosome-associated complex head domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032003">
        <property type="entry name" value="RAC_head"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042569">
        <property type="entry name" value="RAC_head_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054076">
        <property type="entry name" value="ZUO1-like_ZHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044634">
        <property type="entry name" value="Zuotin/DnaJC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058871">
        <property type="entry name" value="Zuotin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43999">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43999:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16717">
        <property type="entry name" value="RAC_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21884">
        <property type="entry name" value="ZUO1-like_ZHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26185">
        <property type="entry name" value="Zuotin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="5">
        <location>
            <begin position="100"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="302"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="346"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="363"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="375"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ86377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="450" mass="50296" checksum="E8944F02346DEEB7" modified="2017-01-18" version="1">MSFLLPGLTIEALEISDNAKSLLSSTVKSVEPVGQEFIHHAQRAIRNHTFSEYEKLLLEKNGGADGDNADNTDPDEALFDDELADASLLKHDPKDWKTADLYAAMGLSKLRYRANGEQIVKAHRKQVLKFHPDKTSAASGGNNLDQDGIFKIIQKAYETLTDVNKRRQYDSCDFVADVEPPAAPSKKAVASGKLPYDFYEVWGPVFASEGRFSKKQPVPVLGDAETPRQEIEKFYNFWHRFDSWRTFEFLDEDVPDDSSNRDHKRYIERKNKAARDKKKTADNARLAKLVERALSEDPRVRAFKEEDKKEKERKKWEKDAGARAEAEAKVKAEAEAKAKAEAEAKAAAENKVNSKKAKEAAKSARKKNKRAIRNAPKDHGYFGDNDKEAVIDEQIGYVIDSMDDEQLADTVSKLADASGNAASVKQILVDIAKVLADSEKLVASTAPYFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHQ0</accession>
    <name>A0A1E5RHQ0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Mitochondrial respiratory chain complexes assembly protein YTA12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AWRI3579_g1444</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="17">Component of the 850 kDa m-AAA protease complex, a heterohexamer composed of YTA12/RCA1 and YTA10/AFG3. Associates with the prohibitin complex, composed of PHB1 and PHB2, inhibiting the activity of the m-AAA protease complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHQ0">
        <property type="interactions" value="691"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHQ0"/>
    <dbReference type="InParanoid" id="A0A1E5RHQ0"/>
    <dbReference type="OrthoDB" id="1413014at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005745">
        <property type="term" value="C:m-AAA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097002">
        <property type="term" value="C:mitochondrial inner boundary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140567">
        <property type="term" value="F:membrane protein dislocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065003">
        <property type="term" value="P:protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000019">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000003">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1690.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050928">
        <property type="entry name" value="ATP-dep_Zn_Metalloprotease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011546">
        <property type="entry name" value="Pept_M41_FtsH_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655:SF14">
        <property type="entry name" value="MITOCHONDRIAL RESPIRATORY CHAIN COMPLEXES ASSEMBLY PROTEIN YTA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06480">
        <property type="entry name" value="FtsH_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="19">
        <location>
            <begin position="449"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="881"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="895"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="20">
        <location>
            <position position="907"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="20">
        <location>
            <position position="922"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00065348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OEJ86449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="926" mass="104043" checksum="8031742F8A61AB16" modified="2017-01-18" version="1">MIIGASSQKLHIKVGQIFAGSILRNQQTRAASVRLSVRCFGSFPSVSTISNSKTKFEAVKNVNSTFSGSRGFHSGVVLRLEEAPENSVDNSKLLKDIQDFKNSLSFRETFELSNRLRVLEKRDIDATSAKRNQIFMEAMQAKTEAERKAKFEEYDKVPKVEVRFFNLTHPDSNKYIVQDKMFKDWLKQKTESQLNMYKEHSQTNGEKAQSNSNNSSKNGKPKIEFVFTKNAKGPEKKNPQNPKPQFRLTFGNAVLIAALVVFLFNVLGGVSSSKEISWQEFRNKILARGFVDKLIVVNKQFVEVELNAQGKSHPEFTQSYYYFNIGSLEKFEQKLSKAQEELQISEEMKVPVLYVQQGSLMKSFYQMGPTLLMLAGMFWLIKKGASAASKQSGGIFQVGKSKAKQFNQDSNVKVKFDDVAGCDEAKEEIMEFVNFLKHPKKYEKLGAKIPRGAILSGPPGTGKTLLAKATAGEAGVPFFSVSGSEFVEMFVGVGASRVRDLFKNARMNAPCIIFIDEIDAIGKARGSGNASNSNEERETTLNQLLVELDGFTPSEHIVVLAGTNRADILDKALMRPGRFDRHIELDNPELEGRKQIFKVHLGKIKYANETEDLVNRLAILTPGFSGADIANACNEAALIAARENANYVRLIHFEKAIDRTIAGIEKKTKVLTPEEKKIIAYHEAGHAICGWFLEFADPLLKVSIIPRTQGTLGYAQYLPGDLYLHSQQQLLDKITATLGGRVSEELNFPTVTSGAQDDIEKITRMATTMVTQLGMSPKIGWTYYSKEDRMRQNDYTKPFSEETGAIVDSEVYRIIQECHDRCTNLLKEKAVEVEKVAQLLLKKEVLLREDLVNLLGKRPFAERNDDFEKYFTKELTDRLRDQEQAVVTENEAEKEDXKSKDESNRXDKKNSDGKDESPSSGEKNEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHR0</accession>
    <name>A0A1E5RHR0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">mRNA transport regulator MTR2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1243</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHR0">
        <property type="interactions" value="170"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHR0"/>
    <dbReference type="InParanoid" id="A0A1E5RHR0"/>
    <dbReference type="OrthoDB" id="25408at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019488">
        <property type="entry name" value="Nucl_pore_RNA_shuttling_Mtr2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10429">
        <property type="entry name" value="Mtr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ86406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="173" mass="19554" checksum="CD3716E5826224EA" modified="2017-01-18" version="1">MNTAVTNNKGNHLIEVLVKKFILLLDETDQTKLTELNNMFSPADCKIIYNNVPYASAVNFLTMWVNKCPQTQHVLQLLDCHIIPGLNHAIXNINCKVKFDETGYTKLNESALIQENHVGGLNRRRPIFGSNFGCSIQLVIDANKFLGQKDQNGVVMSFNYNIVYKPLDSLMQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHW7</accession>
    <name>A0A1E5RHW7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Tat-binding 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1033</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHW7">
        <property type="interactions" value="797"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHW7"/>
    <dbReference type="InParanoid" id="A0A1E5RHW7"/>
    <dbReference type="OrthoDB" id="5421at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140674">
        <property type="term" value="F:ATP-dependent histone chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006337">
        <property type="term" value="P:nucleosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05491">
        <property type="entry name" value="Bromo_TBP7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19517">
        <property type="entry name" value="RecA-like_Yta7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001218">
        <property type="entry name" value="AAA family ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000016">
        <property type="entry name" value="ATPase family AAA domain-containing protein 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000061">
        <property type="entry name" value="ATPase family, AAA domain-containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045199">
        <property type="entry name" value="ATAD2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23069">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23069:SF0">
        <property type="entry name" value="TAT-BINDING HOMOLOG 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="12">
        <location>
            <begin position="1191"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="40"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="88"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="451"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="520"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1279"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1355"/>
            <end position="1378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="88"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="191"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="261"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="278"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="290"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="476"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1362"/>
            <end position="1378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ86474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1471" mass="164777" checksum="F0288C0BB9AE3009" modified="2017-01-18" version="1">MSDPNIAYTNALDDHVSHHTRNLRKKVNYAELENSYDFVDFDDPPDAETDVNGGNLEENNGTSVSHAAESDEDINVIRRSTRASQGLINNINNNLGGNTNNEGVDEIGENVNNTRSRRSTRFAQNNHGNDENAYEQRFNNNINNNNGNTNKHGDDDDDDDDEYHYDANANAEDNEDYVYSTRRSSQKRGRTASTRNTRNSRYRTSGNSALNDFIEPDEFDEGKLDNDDSDDYGYSTTRRSKRGRTTKGQQSATTGTERLTRNSGRRTASTRSTSRNNTRSRRLKRRGVNNDEDEDEEYTEEDKPFAASDEDISLKQELQELQEDSPVNSPKRSLRKRTKNVNYVLPLPTDLDNNLEEQTDGYYANNSNQPIYGASSNAYPSQSIISPRRRLYGGGKKNGLGAKTGPVRKLFATGGPFGGNDVTSIFGGVNVPTYIPSPTENDVAHEQTHIDTFNDDPTTKLVGTSIDANGPTVPPSLASNSTNFANNASLNPANNKKNTLILDSDSSDDEILPFGEASKKNANKKSSSKSLFDANGNALTKKPETADVDPLGVDMNIDFNDVGGLDNYIDQLKEMVALPLLYPELYATFNITPPRGVLFHGPPGTGKTLMARALAASCSKASGQNITFFMRKGADILSKWVGEAERQLRLLFEEAKKNQPSIIFFDEIDGLAPVRSSKQEQIHASIVSTMLALMDGMDNRGQVIVIGATNRPDSVDPALRRPGRFDREFYFPLPDVKGRNKIIQIHTKKWKPPLPQKMIDKLAELTKGYGGADLRSLCTEAALSSIQRRYPQIYQSSTKFKIDPNKVHVSAKDFMLALEKIVPSSARGGSSLQQPLPKNIECLLHRQLTHLENELELILPKDSIQKKHSDSLIKHYLEHDETVSDDDEENQDFKTDGGFGKQEFLKELKDSRVSKPRFLLCGKRGNGQQYIGSALLNFLEGINIQNLDLSTIMSETTKTMEASTVQVFTEARKRQPAVIYIPNFDVWIRTVAPTVVYTLTSLLRSLRADEKVLLMAIADGQLPTEPPLIASLIGEFEFETAFELETPNAEQRHAFFSELKTLLCTKPTKFSSNKKRVKPVERLPEAEKSLDPDLVDENGAVLSEEQRLEKSLKKFQHLDMKLKNTLKIKLSGLMELFKNRYKKFRKAPIDDIYLIHLFEPLPEPAEYYQPAYVKDGEKILEVATGKRFFNMDLDVIEERLWNGFYSEPKQFLKDIEMIYLDAHVLGDRERIIKSSEMFANAQVGIEEIATPEFAEQCKATRKRENLRLKLYLKKQASEEDAEQESSAVQTNFVPEVGADPPAKSLVSSNTVEVTVGAGSQVQSQLQKVPEETVSTELSRDDNLALPLEVVNVPATESAPLKNETQLSNDANSPKLPNSLAASESLENAVEPLHTQVPANEEPKKSFPDPEFIVNDVLLTDIIERLTVVTNGWPVQMIQQKYAKLVSCIWKFRYEWDKKEAIEALKELAEQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI00</accession>
    <name>A0A1E5RI00_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">General amino acid permease AGP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1611</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI00"/>
    <dbReference type="FunCoup" id="A0A1E5RI00">
        <property type="interactions" value="272"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RI00"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF17">
        <property type="entry name" value="GENERAL AMINO ACID PERMEASE AGP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="328"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="380"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="428"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="451"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="502"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="535"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="7">
        <location>
            <begin position="96"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="607" mass="67442" checksum="E2845783583C94A6" modified="2017-01-18" version="1">MSYITSEKSPAPVFSKEEDTIRVDESLEEMSYNEPSSSNKKPFLNSKRLQGMIDSFKKPEESNHLGESNDLEVSPSNEIDGFGKKTELQQKIKPRHIFLIQLGTGIGTGMLVGNGSALRQAGPAGLFIGYAVVSSFIYCTYNSISELGISYSQLSGSYNEFSCILIDRAFGFSVAVVYALQWLSVMPLELTTSSMVIKYWTTKVNSDVFVVIFYVLLLMVNFIGGSGYAEFEFIVNTMKVLMIIGFIIFGIISDCGGSSGNNHTYIGGENWRAPVGAFFGKSKADHFKGVCSSFVYAAFAYGGFESSILTASEQVNPRKSIIKAGKMLIYRIIVIYCLTVVIIGLLVPHTSSRLLGTGSSATAASPLVIAASNIRGLPHIINAVIMMAVLSVANNSLYCSSRVILSICEQFFPNVPLLTYVDRRGRPLFGLILAAIVGCIAFVACSPKEDIVFTWLLSISGLSALFTYFSMNMSHIRFRKAMKVQGKSLNEMGYISKTGLWGSYYSCIVIILIFIAQFWIALVPVGTYKPDANNFFQNYLAMVVFVVLYFGFKIITKEWHIFIRPEEVDLTRGRRIFDHDLIVQEKLEEDERLKHSSIFVKILNFWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI67</accession>
    <name>A0A1E5RI67_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">54S ribosomal protein L6, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1108</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RI67">
        <property type="interactions" value="731"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI67"/>
    <dbReference type="InParanoid" id="A0A1E5RI67"/>
    <dbReference type="OrthoDB" id="540873at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="entry name" value="Ribosomal protein L6, alpha-beta domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_uL6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_uL6-like_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_uL6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019906">
        <property type="entry name" value="Ribosomal_uL6_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002358">
        <property type="entry name" value="Ribosomal_uL6_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="60S/50S RIBOSOMAL PROTEIN L6/L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF14">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL6M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00059">
        <property type="entry name" value="RIBOSOMALL6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="Ribosomal protein L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00525">
        <property type="entry name" value="RIBOSOMAL_L6_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="5">
        <location>
            <begin position="60"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="5">
        <location>
            <begin position="132"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ86283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="220" mass="24724" checksum="AC45BCAF9AA706F1" modified="2017-01-18" version="1">MLSNRFFLTQKSLLHTSSVCRSYIGKMPVLLTPETSVSIEKLSEPIKRKLPYEKIMLFQNKVKVVGPKGELSLEVPDFMKIEQNAEKTNFTVSVXDSSIPVQRSMWGTVRSILKNNVIGVNEGHLAMLKFVGTGYRCQILEKDNYISIKVGNSVKQGIEIPAGLTVTSPTPTFLIVEGIDLQKVNSFAMNIRKFHPPEPYKGKGIYVNDEKIKLKVKKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI77</accession>
    <name>A0A1E5RI77_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Putative transcription factor TDA9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1181</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RI77">
        <property type="interactions" value="831"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI77"/>
    <dbReference type="InParanoid" id="A0A1E5RI77"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051059">
        <property type="entry name" value="VerF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626">
        <property type="entry name" value="MIP31509P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626:SF13">
        <property type="entry name" value="RESPIRATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1381"/>
            <end position="1400"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="103"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="163"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="757"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="854"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="219"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="296"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="760"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="773"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="854"/>
            <end position="868"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ86601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1523" mass="172738" checksum="20095599F5CC29FE" modified="2017-01-18" version="1">MVSVKNEEVKQYLTNSEDIQNQPYNAQQANMTEPNPLILKTEQPSPESSISQPIVTVATAEGKKTDVSAASKKQASSLEIAPETNELVPRNSRKVRTDKPRPFICEICLRGFVRREHLKRHSLAHTQARQFDCVYCGRPFTRRDMVLRHQRTLHWQLFTDVDEKNKSSDNTPTTQKKIRKHKEKTIGEDGKVLLSKRPAKTDNNTDDSYRAKTLKKQADISSSSTTTTPITDSNEQDGEENGDELLEMKPRKRPRHASFSASSGSTYTFTTPISHGSDLNTLAGFAQNRGFVTSNTTDENTYXTHTQNNNNNNNNNNXQYTLSATLAAPETSTSLATTPFQXMTPHGQDVHYQEAISATETRPHQQVEFSTPQMKAQDMALLDFLLPDMQLDQDEETGVSEFQNYNFGSAINSSANLHSKYSAEADALNAERLNTVLKNIDSILPPKHFTMEYFHTAQNAEKQIATSNNSNXLHGKPSSLDQKNYPIHQDQNNLKTHSKQDNPQNGDTFLTDKSMKRNKSLLNLNLSQLPLYSTILSSFPDQAPGNMSTANQLAQLQYYQTAGEMAVQNAHNKPSELGKSTHLAGXHVFSPETCNPPSTANFLAFGNHGTVSEQFIDKKYHLNQYNQYDFKANSAPVEDMMMDLLQKNSANTQELLEGEAPASWLSDFINTHFEDELKDSNXLNTHFNDIGFDENSYANTNLSSSNSYADINLQSHLTQVPPSQPQFETKPPVLDLDTNNLPVKYMPPFTSPLQQLKADASSTYFQPSSTHDQXQQQQQQQQXTKTSNAELVPGLFKQRQMDLWKQFFPRTTSPSDFSSPVVSSTVSETGKLTSSHQASAIPSPLAKTNVFTPTSSIPPSDLTTQTASGXAKVQEKIKTKKPVSKPRVGKLKWFDEKLRVQIMQLHNLTQFPRVKELNEYINLYQREFHPYFDFIHLQSITPKLENHSILTSVACIGALYSFHSYHSMQLFSISRHNIREMLEEFSDHHKGQDILAPDFNLNISKDHINKMYESSYYDGGELPLWLIQAMVLLSFVEIFHSSSSINEQVATHLKTVIKLIQIKNLNKPLEKIINPPIDFNNMKANKIGDENLKEIFDYFITAQSRIRTCHTVLLISNLFTSLVGLDCRMHSVDLKEGGIPCLQEALFHAANYKEWSKLLRVQYKIRLDSKFTLVELSNGGDDYTHCLRYLSNNALSSMTDGVGNEVTFELDDFHSRRLSHSTLLSMLLSIHEKIFIERSKNYNTIQWELKSKPIIRQLMKTWEHLYLKNGGVFYDSSNLDIIIQNPKMKLLLPLYNFAKLRKCLNIAPIMKKIWLKNWAKLNEILDDLMFTCCXNDELSAESYNKNLMREASLYCLDTIKLWIETVSFANNSEKSSINTPIFFITCIFSSIVILSVYLQVLEYMATKVVLVESDKKFWMQTEMVLQKLENHLSTNKSQIDSTVHPVVKKPLAFSXEFLQKIAEDDVQFNKMVVSTKLSAKCLYLGVRILGDAPVWPASLNFASGLFSRCQHITTAEVFGEYSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN21</accession>
    <name>A0A1E5RN21_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA-directed RNA polymerases I and III subunit RPAC2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g713</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal Rpo11/eukaryotic RPB11/RPC19 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN21"/>
    <dbReference type="FunCoup" id="A0A1E5RN21">
        <property type="interactions" value="619"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN21"/>
    <dbReference type="InParanoid" id="A0A1E5RN21"/>
    <dbReference type="OrthoDB" id="510325at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055029">
        <property type="term" value="C:nuclear DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07029">
        <property type="entry name" value="RNAP_I_III_AC19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.10:FF:000012">
        <property type="entry name" value="DNA-directed RNA polymerases I and III subunit RPAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00261">
        <property type="entry name" value="RNApol_arch_Rpo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009025">
        <property type="entry name" value="RBP11-like_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008193">
        <property type="entry name" value="RNA_pol_Rpb11_13-16kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033898">
        <property type="entry name" value="RNAP_AC19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022905">
        <property type="entry name" value="Rpo11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I,II,III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946:SF28">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13656">
        <property type="entry name" value="RNA_pol_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01154">
        <property type="entry name" value="RNA_POL_L_13KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RBP11-like dimerisation" evidence="6">
        <location>
            <begin position="45"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="129" mass="14683" checksum="D429873556A60D8F" modified="2017-01-18" version="1">MATEQKDVEMIEENNSVVENNEPQALADHNKIRLLPEATSEDGTCASFQITDEDHTLGNSLRYMIMKNPEVEFVGYSIPHPTENFLHLRIQTYGQITAVDALHKGLQDLMDLCDVVEEKFTENIKSFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNC2</accession>
    <name>A0A1E5RNC2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA polymerase II holoenzyme cyclin-like subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g845</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family. Cyclin C subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNC2"/>
    <dbReference type="FunCoup" id="A0A1E5RNC2">
        <property type="interactions" value="985"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNC2"/>
    <dbReference type="InParanoid" id="A0A1E5RNC2"/>
    <dbReference type="OrthoDB" id="10266018at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20513">
        <property type="entry name" value="CYCLIN_CCNC_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000077">
        <property type="entry name" value="RNA polymerase II holoenzyme cyclin-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031658">
        <property type="entry name" value="Cyclin_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16899">
        <property type="entry name" value="Cyclin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="6 10" id="KW-0195">Cyclin</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Cyclin-like" evidence="12">
        <location>
            <begin position="138"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="51"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="352"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ88389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="467" mass="52258" checksum="18B5FA559F7BF71A" modified="2017-01-18" version="1">MSASYWTSTQRNIWTFTKQELARERNKLWFLESQTFADGLTVPMVNNSSGSHGGLNAGGSNQDTGGAGTNLGDSIASSNKTNGSSTNVSAGNGMNKKNGASGSLSSNSNGRIVSKHISIYSKDLHYDKDYNLRIYCYFLVMKLGRRLNLRQIALSTANVYLSRFLLRVSVREVNIYLLVTTCIYLACKEEECPLHIRSLVNEARTNWPEFISTDATKVTEFEFYLIEELGSYLIIHHPYHSLEQLMNSFNEKVKQTALDNGAIGDDLNGDTDNDPMMIDLTKADGNEHSSQATFGEDSTYKNLLLINAQIYQNAWSLINDSYITDVHLLYPPHVIAVACLMISLSSGTSPHCDSYTSEQSSGSSSSSSSSIPSAILDTKPHGTTGNGGNQQYHFKAKGHKSPSPSKKTQERNQKIMKKLHVFLAESLIDMEMVLQCCEELLNLYDKWEKYNEDWTRYLLYHVYLVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNC6</accession>
    <name>A0A1E5RNC6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA polymerase</fullName>
            <ecNumber evidence="12">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g699</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="12"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RNC6">
        <property type="interactions" value="952"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNC6"/>
    <dbReference type="InParanoid" id="A0A1E5RNC6"/>
    <dbReference type="OrthoDB" id="6755010at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05776">
        <property type="entry name" value="DNA_polB_alpha_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05532">
        <property type="entry name" value="POLBc_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3200.20">
        <property type="entry name" value="DNA Polymerase alpha, zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024647">
        <property type="entry name" value="DNA_pol_a_cat_su_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038256">
        <property type="entry name" value="Pol_alpha_znc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045846">
        <property type="entry name" value="POLBc_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015088">
        <property type="entry name" value="Znf_DNA-dir_DNA_pol_B_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00592">
        <property type="entry name" value="pol2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45861">
        <property type="entry name" value="DNA POLYMERASE ALPHA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45861:SF1">
        <property type="entry name" value="DNA POLYMERASE ALPHA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12254">
        <property type="entry name" value="DNA_pol_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08996">
        <property type="entry name" value="zf-DNA_Pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 12" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase alpha catalytic subunit N-terminal" evidence="17">
        <location>
            <begin position="12"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="15">
        <location>
            <begin position="503"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="14">
        <location>
            <begin position="817"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger DNA-directed DNA polymerase family B alpha" evidence="16">
        <location>
            <begin position="1316"/>
            <end position="1508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="168"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="168"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="195"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="213"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="238"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="258"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="275"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF12254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ88387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1514" mass="171906" checksum="B4C3DD311AC7E03B" modified="2017-01-18" version="1">MSSTKEDRLKALRKLQKKRSGHFSDSEQESDYDAGKTHQESDNEQLYDVVDEKSYKDQRRQAMLNDNFVVDDDGDGGYIDNGVDEWDREDQYYSDENMEPESSKKASKSKKTGKSSKPDISSLINKHAAKSYGKRGSTASGFDSIGRKAHKVSVDMFDDIFNEFGEEKVNKNSTEKKKNFKRKMESTANGNIASDDFHDEITPETTKKLKFSSPITTTFSQDKNRNTSAQPVSSPLKQKWDANLSKKDEMDSPTDENAYGREKSVSNHDATSSKDDDDDEDDEDEILTLRRKRTVRAGNVSRTVNMKSGNSSFVTAPSTPNTSIQNISLDLDASSPSTSTVSSSFVSSSFNRFSKKLDKETIFTSKDGSIPFYWIDYSEINNTLVLYGKVKSTDGNYCSCMVQVEQMYRELYFLPKTGETPADIYDLVVSTISEKYGLPTLRAKPEKMKYAFELPGIPKRETEYLKVLVPYSTPKNSFKKITLDMFKQKTLNNQEEENFPIQHIFGASSSIFESFVVQRKIMGPCWLALRNVEFDKLTGASHCTAELKIEKATDISVLEGNAFQLPPLSTITLSVQSVLNVKENKQEIVSISYKLNPKLDLDYDVADVSSQNLETVTLMRPPSFSTFPVSLKKLAESKFPTNPVRLFNNEKGLLSCFAAILKKSDPDVFIGHKLETVGLDIIMNRMFDLKIANFSSIGRLNRREWPDRWLRNRNHQFYLIRDILSGRLVCDIANEMGQSLTPKCQSWDLSEMYNIYTGNSYKPLEINFNQVQYQEDASSLILAIEENISNCKMVDEVANKIQILSLSKQLTNLAGNNWNQTLSGNRAARNEFILLHEFAREGFILPDKEYKNNADQTNSQGRSKKSKYQGGLVFEPEKGLHKNFVLVMDFNSLYPSIIQEYNICFTTVDREHLLSPSSADSEEEALPEIPSVTKEQGVLPKLLNNLVARRREVKKLMVSESDPFKKAQCDIRQQALKLTANSMYGCLGFVNSRFYAKPLAMLVTNKGREILMNTRQLAESGGLKVVYGDTDSVMIDTNKNVYSEAIEIGQEFKKNVNDRYKLLEIDIDNVFKKLLLHAKKKYAALNCSIDPKTHMERLLLEVKGLDMKRREYCPLSKEVSEYVLNILLSDKFSDSEAALSEVYTYLEDISDGIENSKFKFDKYRINTKLSKDPTQYPGGNSMPAVQVALRMRKTGRIVKAGSVITFVITQPLDPEDQEKDKNGVSSDSTKDEQHFAARARAFTELLAKNSNLKPDAQYYLEKQIFTPIKRLLENIEGFDVVRLAESLKLDGKKYLASISSSGASANGINEIQPLESTVTDNERFRECKAFVVHCSHCSSNFKYHGIVKSADYTVSFNGIKCQKCDTQMRLIQVTSQLESRIRGFIAKYYEGWLQCDDSTCNCTTRSIGMYGKKCLNEGCTGVMNFKYTDKMLYNQLLYFSSLFDYEKNKQQKLEIFANTAEDEDHKLSASELKALVEQNREMMFVNQQVVQKYLNDCARSYVNMTDIFSFSARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNQ6</accession>
    <name>A0A1E5RNQ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Guanidinobutyrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g762</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arginase family. Agmatinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNQ6"/>
    <dbReference type="STRING" id="56408.A0A1E5RNQ6"/>
    <dbReference type="InParanoid" id="A0A1E5RNQ6"/>
    <dbReference type="OrthoDB" id="288726at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033389">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via agmatine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11592">
        <property type="entry name" value="Agmatinase_PAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.10:FF:000014">
        <property type="entry name" value="Arginase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="ARGINASE/AGMATINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358:SF26">
        <property type="entry name" value="GUANIDINO ACID HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00116">
        <property type="entry name" value="ARGINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184820" evidence="5">
        <location>
            <begin position="20"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ88504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="427" mass="47477" checksum="486022BC358D00FE" modified="2017-01-18" version="1">MQLLATSSILLAFAHLCKAHGNHHSDESSFNPVDFADEEASLEDIWGGFWPYQGVETFAHLPHTECLIDDQETFDIGIIGVPFDTATSYRPGARFGPSGIRKASQRQDMWRGFNFRAGLNPFDDWAKIIDCGDIPVTPMDNALALKQMSAAYDELVLKRNSTFDYSQSGSQKTSPTRVPPRFVSLGGDHSILLPILRTLKKAYGEVYVVHFDSHLDTWAPDKYPSYWKSEASDFTHGSMLWLAQQEGLIAKNGNLHVGLRTRISGDSWDDFDEDDEVGFKRIFADEIIDLQATGMARKILDYLPHDKPIYVSVDIDVIDPSAAPGTGTMEVGGFMTRELIYMLRQMETLNIVGADIVEVAPAYDPLDITQLAGSQVAYELITSMVKKGPLDTSLLADPATVATDEVDIIDKLINDESRIANMRMPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNY3</accession>
    <name>A0A1E5RNY3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cysteine protease</fullName>
            <ecNumber evidence="11">3.4.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g590</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[protein]-C-terminal L-amino acid-glycyl-phosphatidylethanolamide + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine</text>
            <dbReference type="Rhea" id="RHEA:67548"/>
            <dbReference type="Rhea" id="RHEA-COMP:17323"/>
            <dbReference type="Rhea" id="RHEA-COMP:17324"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:172940"/>
            <dbReference type="ChEBI" id="CHEBI:172941"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67549"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Preautophagosomal structure</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase C54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.-" evidence="11"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNY3"/>
    <dbReference type="FunCoup" id="A0A1E5RNY3">
        <property type="interactions" value="309"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNY3"/>
    <dbReference type="InParanoid" id="A0A1E5RNY3"/>
    <dbReference type="OrthoDB" id="2960936at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019786">
        <property type="term" value="F:protein-phosphatidylethanolamide deconjugating activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035973">
        <property type="term" value="P:aggrephagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005078">
        <property type="entry name" value="Peptidase_C54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046792">
        <property type="entry name" value="Peptidase_C54_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624:SF49">
        <property type="entry name" value="CYSTEINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03416">
        <property type="entry name" value="Peptidase_C54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 11 13" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase C54 catalytic" evidence="12">
        <location>
            <begin position="84"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ88594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="534" mass="59832" checksum="30DE9340489E68F7" modified="2017-01-18" version="1">MQPTNTSEDKNLTTEEDPNATPLVCILGTIYEPEKYAQTHTKKAEEPAEPDLFMKVNNFMTSMLENTASNLTSMLTPTNNLHDEAIMKDIYTVILFTYRTDFEPIAKITGNEDDHAISVLLRAGVFAYYSNIVKNNANNSFITDVGWGCMIRTGQSLLANALQIIHYGRSYRYSLEASDNDTGKQADSSLTGGNFMNWFRDEFQYPFSIQNFVKVGYELSNKKPGEWFSPSAAARSIQKLVLSFPECGINNCYISIDSGTIYLDEVDRMFEKDDSLSRKTNVLILLCVRLGLSNLNEYYWNDIKGLLGLEGNTCGISGGKPSSSFYFFGYSNDVLLYLDPHSPKLHTKDPTQFAQSAHSTDYKTLPFSQVDPSMMLGFLIQGKEDWISFQEKIRAMNNNKGSRIVQFTNHPEYGGRDSVALDQHPPVSAGETESSYVECGSDAATRGTLERTESIGFASNGAAEVFSDTEDYVDIGEVLGNMRLKKASFDQEYDEFQEVQCKDQKIVTLKNDSDDVDNEEKVLCEQETVSLGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP02</accession>
    <name>A0A1E5RP02_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">26S proteasome regulatory subunit RPN6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g934</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP02"/>
    <dbReference type="FunCoup" id="A0A1E5RP02">
        <property type="interactions" value="1196"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP02"/>
    <dbReference type="InParanoid" id="A0A1E5RP02"/>
    <dbReference type="OrthoDB" id="1418352at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050871">
        <property type="entry name" value="26S_Proteasome/COP9_Components"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040780">
        <property type="entry name" value="Rpn6_C_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040773">
        <property type="entry name" value="Rpn6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10678">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 11/COP9 SIGNALOSOME COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18503">
        <property type="entry name" value="RPN6_C_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18055">
        <property type="entry name" value="RPN6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="249"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ88609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="456" mass="51833" checksum="DD573CC2B839E1DE" modified="2017-01-18" version="1">MSLEQADTYFQSANTYPQAIKEYTSVFQSNKDKILTITSSNAVSSAISKILQEQEHVIIKLGELYGVTQNLSELIQLIEFSKEFMMTYAKSKIAKILKVLIDDFEMIPDSIDAQIKVIEESIDFADKHKRIFLKNSLSIKLANLYYDKQQYSLSLNLINTLLRNFKKLDDKSSLVDIHLLESKVYHKLRNMPKAKAALTSARTAANSIYCSSATMAELDMISGTLHCEDKDYKTAYSYFFESFDAFHNDSTKSTSALKSSKNNGNKKISKDRSNKLFVKSEQVLKYMLLCKIMLNLIDDVNQILNAKYTKEIYQSKSIDAMKTIAEAYSDRSLLEFNKALKIYETELKTDDLIRSHLNALYDALLESNLSKIIEPFDCVEISHISNLIGLDYKQVEGKLSQMILDKVFNGVLDQGNGWLNIYGNEVCDPTYDSSLELIGNLNKVVEQLFEKAGVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP42</accession>
    <name>A0A1E5RP42_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">INO80 complex subunit B-like conserved region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g603</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP42"/>
    <dbReference type="STRING" id="56408.A0A1E5RP42"/>
    <dbReference type="InParanoid" id="A0A1E5RP42"/>
    <dbReference type="OrthoDB" id="2021186at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006880">
        <property type="entry name" value="INO80B_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04795">
        <property type="entry name" value="PAPA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01406">
        <property type="entry name" value="PAPA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="INO80 complex subunit B-like conserved region" evidence="2">
        <location>
            <begin position="283"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="338"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="430" mass="49627" checksum="14F4EB1EE9544955" modified="2017-01-18" version="1">MPINRGQRKRYISDSENSDDDEEIVARRTSKRSSAGKRPSPVYGDDEDENGDEDEMLYSNSGRPIRSSSRLSSRKIDFKEPNEDEFDSEPEDVPAKWNEQKEIEKDETQLNENEGEKVNAGHADNDDEDEISNDENEQVGEDGDDIVADKNEEVEEDQEDENENENENEEEDEEEEDEEEGEGEDEDDEDEDEEEEEEEEEEEEEDIPIDDDENDDTSIPAIVDDDDMDSLEQSNNERGSVSNRNQRLFSKTSENDETLNNQNAAEDGNENEDEEDVENKMSEKEIRRTENLRRRKVLHDKRMEEEKRDTINKLLKRRAGKSRSKDSGSKKSAALQQPGGSVNGNKSTSSLASDGTEGNHGKLDKEEEEEEEEEEGYKVNKPRRKYDNRGMRRIVYGRDNTIRYAFEYQDKNGAFQHEASERQNDMVSVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RPC4</accession>
    <name>A0A1E5RPC4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein MKT1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g772</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPG/RAD2 endonuclease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RPC4"/>
    <dbReference type="FunCoup" id="A0A1E5RPC4">
        <property type="interactions" value="391"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RPC4"/>
    <dbReference type="InParanoid" id="A0A1E5RPC4"/>
    <dbReference type="OrthoDB" id="17262at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09858">
        <property type="entry name" value="PIN_MKT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022039">
        <property type="entry name" value="MKT1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022040">
        <property type="entry name" value="MKT1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF32">
        <property type="entry name" value="POST-TRANSCRIPTIONAL REGULATOR MKT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12246">
        <property type="entry name" value="MKT1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12247">
        <property type="entry name" value="MKT1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ88543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="832" mass="95204" checksum="E9E0994F3CFD5016" modified="2017-01-18" version="1">MPIRSLESFLFERNLVDTCPIDSISGCRLGIDVDHYFSKLLLNKKELFTDAIGGCPTGLIVHIENDLKFFKENEITPVFVFNGFETVNQVEYSSKLFTNPKFVPQSTSTSSPESTAQEKSIFVSSPLLSVSGDSVVASSAERMVASDRGTFKQIVDYQRIQGWSAWKKLLEETKGSYVDQPPTPTQPFKFETKFLEINRFKNELIQILINKEIEYLVAPFVSWIQLAYLFENQLIDAIYGSTDLLLLEKLDKFILSISFLGKEVRYVEKASFLKETKISFNDFVDISMCCGNSLQPETLLSLSASVPDQQIFAYALDLVSTQGFDLYVNLKNSNEQKETDYFERGLATLSCSPIMSMNGKVILYSTQLPADTNTTSTTDSGTEQRIPKNKVPKNVHDYISKRLPDEYHFYFMIGLFSNTKVLDAIISNQYVENLPLAAKNIAEYQELVRKSSSVFKNNQFNILASSLNRYYQSRPIKHIKWFEPQNSEELPSRLDSNLFDEISKIYVKTDNEKPFTLANFVALFQNQTEWKTKFFNQKVIFPGNVPITDKLNTSFDLLSTSLLRFLFLIGFFELKLDATKTKATRLKATKWGDVFLKLKDITGVDQDMESLLLFLILMKFQVIKLNATFDDNKYPSSLSESTKTQHPMESKNISLLCRVLLLCKSKNNPECYQGPVDYSLLCFRDFYENVSQNSRELLESCTASTLVYGEFNRVSLKSNNEWSQKIVKQMLFKEQTPNTLFTMIWEFYLQKMLHSGNSHKEAVQLISKTFNTAQFSTEGEIEKSVLRKGLIFLQSIQSLFNELTQKKLVDETEAEALNKAVTWVEEIVGNYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RTS6</accession>
    <name>A0A1E5RTS6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NADPH dehydrogenase 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g571</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000004">
        <property type="protein sequence ID" value="OEJ90246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RTS6"/>
    <dbReference type="STRING" id="56408.A0A1E5RTS6"/>
    <dbReference type="InParanoid" id="A0A1E5RTS6"/>
    <dbReference type="OrthoDB" id="3969599at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045247">
        <property type="entry name" value="Oye-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893">
        <property type="entry name" value="NADH OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893:SF91">
        <property type="entry name" value="NADPH DEHYDROGENASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="4">
        <location>
            <begin position="7"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ90246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="132" mass="15152" checksum="7430CB6D7C5FACBF" modified="2017-01-18" version="1">MDKLHAHFIGELEKRAKENPENRLAYVHIVEPRVPSPAFGEGEHEYLEGTNDFVFSIWKGIVIRAGDYALNNTEALKDVNENNRTLLAYGRFFISNPDLVDRLEKGLPLNAYDRDTFYTPGPKGYIDYPNYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV56</accession>
    <name>A0A1E5RV56_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Homocysteine/cysteine synthase</fullName>
            <ecNumber evidence="4">2.5.1.47</ecNumber>
            <ecNumber evidence="14">2.5.1.49</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">O-acetylserine/O-acetylhomoserine sulfhydrylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AWRI3579_g509</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-acetyl-L-homoserine + hydrogen sulfide = L-homocysteine + acetate</text>
            <dbReference type="Rhea" id="RHEA:27822"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57716"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="2.5.1.49"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-acetyl-L-homoserine + methanethiol = L-methionine + acetate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10048"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16007"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57716"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="EC" id="2.5.1.49"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate</text>
            <dbReference type="Rhea" id="RHEA:14829"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.5.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homocysteine from O-acetyl-L-homoserine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="4"/>
    <dbReference type="EC" id="2.5.1.49" evidence="14"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RV56"/>
    <dbReference type="FunCoup" id="A0A1E5RV56">
        <property type="interactions" value="201"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV56"/>
    <dbReference type="InParanoid" id="A0A1E5RV56"/>
    <dbReference type="OrthoDB" id="3512640at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003961">
        <property type="term" value="F:O-acetylhomoserine aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071269">
        <property type="term" value="P:L-homocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000083">
        <property type="entry name" value="O-acetylhomoserine sulfhydrylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000035">
        <property type="entry name" value="O-succinylhomoserine sulfhydrylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054542">
        <property type="entry name" value="Cys_met_metab_PP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006235">
        <property type="entry name" value="OAc-hSer/O-AcSer_sulfhydrylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01326">
        <property type="entry name" value="OAH_OAS_sulfhy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43797">
        <property type="entry name" value="HOMOCYSTEINE/CYSTEINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43797:SF2">
        <property type="entry name" value="HOMOCYSTEINE_CYSTEINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="8 17 18" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="17">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00066529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00080931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OEJ90822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="445" mass="48320" checksum="D86D1DBF9A9274BB" modified="2017-01-18" version="1">MPSHFDTLQLHAGQEPDNDNNRPRAVPIYATSSYVFNDSKHGAQLFGLETPGYIYSRIMNPTNDVFEKRIAALENGIGALAVSSGQAAQTLAIAGLAHAGDNIVSTSYLYGGTYNQFKVAFKRLGIEARFVDGDEPEDFEKLFDEKTKAVYIESIGNPKYNVPDFEKIVAIAHKHGIPVVADNTFGAGGYFVKPIDHGVDIVTHSATKWIGGHGTTIAGVIVDSGKFPWGKYPEKFPQFSKPSEGYHGLILNEAFGPAAFIGHARTELLRDLGPALNPFGAFLLLQGLETLSLRAERHASNALKLAQHLEKSPYVSWVSYPGLESHPYHEAAKKYLTNGFGGVLSFGVKNLKDEKAVAAAKEDPFKDSGAQVVDALEVVANVANVGDSKSLAIAPYFTTHQQLSEEEKLASGVTKDLIRVSVGTEFIDDIIADFEQSFKKVFGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVB7</accession>
    <name>A0A1E5RVB7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">54S ribosomal protein L1, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g385</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVB7"/>
    <dbReference type="FunCoup" id="A0A1E5RVB7">
        <property type="interactions" value="299"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVB7"/>
    <dbReference type="InParanoid" id="A0A1E5RVB7"/>
    <dbReference type="OrthoDB" id="1747252at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_uL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL1M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ90765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="285" mass="31701" checksum="3E5D9232B4CBB8A8" modified="2017-01-18" version="1">MMNTVVLRSYTASELFCTKRFISTSSILLGPKSNDAKVARDKSKKKELRKLAMNKNIAKDPAYNQPLYMPLDMALRYLRAAEVGYPTSQQTISITTKIVRDKGTPNLRGKVFFPHSIESENSKSKIMVFTNNDQSQIDYYKNELGCDYVGGQDLVDAIQNDKINLDEIKLSFATPDIASSLNKLGKKLGPKGLLPSTKRGTVAENLTNIIKENSASYPFTQKRADAISFPVGRLTFSDLKLAQNINAVRDGLYKSLSEQQSRKPSIVGHTYITSTNSPAITIDFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVJ5</accession>
    <name>A0A1E5RVJ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g459</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVJ5">
        <property type="interactions" value="86"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RVJ5"/>
    <dbReference type="OrthoDB" id="73788at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="340"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="357" mass="40681" checksum="AD10CF7FAFEB8E1F" modified="2017-01-18" version="1">MDYAYEPTIDEKTRXDSEKEVAGNNHNTIVQDAKTEETFNKLEQKLDQSFTKTTNFLYNLWNEDQHLSLFTEEEDKNGSPGSKTPSKDSSEDIVTRGERAAQHVLDAFDTKLQVVEDFMHNKDQQQKAAENATKLIKDKWSTWSTGLTQFSESLKTKLNDVSGVLAFPESEEHAXETSQRLQVLGTDENIYLNYKGDVNKNDKFSNDDISREVARDINLKVLADSLVAGNKIDYKTFWSIYFTEKQKILNNHSLSTLSQGSVPXMETXLPSWDDEDEEEDENADHNNIETNAVSDSSNEPFDSNDIRLESRESVVIVNKGKDTVVEKSITPGQSEFKNGEEEEEEDEEEADDDDDWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVL2</accession>
    <name>A0A1E5RVL2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g473</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVL2">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RVL2"/>
    <dbReference type="OrthoDB" id="4069967at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16588">
        <property type="entry name" value="zf-C2H2_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="21387" checksum="CD9E47B9464B743A" modified="2017-01-18" version="1">MEEHNAGNDSSNNELIDEMNKLVKLVIKKQDESLRLEKQYETKLKNLTLLVEYLMNLRSTSAKSETDDXXXXDDXNSXTDECGTNAHSTSESDVISKINTTALDDILLNGLEYCVKDGKKYCMIPVINTATEPNTKRVSQSSTKTGIINSPMTPSTHQKKKKKNKIPCSYCKETGHTRSKCQKRLQTEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVP6</accession>
    <name>A0A1E5RVP6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DNA repair protein RAD33</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g559</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVP6">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVP6"/>
    <dbReference type="InParanoid" id="A0A1E5RVP6"/>
    <dbReference type="OrthoDB" id="4085867at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014841">
        <property type="entry name" value="Rad33"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08730">
        <property type="entry name" value="Rad33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="unsure residue" description="D or N" evidence="1">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ90929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="202" mass="23354" checksum="9C31135A06B68A39" modified="2017-01-18" version="1">MSSKKKSSRPQQLNYXKIEQFTNPKSVPKDIEEEVLTCYVDFNIEHDMTIYDLQPFFEKLQLPHNIYKFVMYKGEKELCVEGTNIIDFDKLLIETYHLLIYMDNVAVIDEYWSLLVESCQRDQQFPQVKLKDHVIGLKQLRAIINNVNGSGEEETASGSTMPLIQMMKAVSSPPQKSGADKLFINYLDFAFMLGKIGLLKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVS5</accession>
    <name>A0A1E5RVS5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Heat shock protein SSA2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g492</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVS5"/>
    <dbReference type="FunCoup" id="A0A1E5RVS5">
        <property type="interactions" value="2161"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVS5"/>
    <dbReference type="InParanoid" id="A0A1E5RVS5"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10233">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000172">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000001">
        <property type="entry name" value="heat shock 70 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000016">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000026">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="609"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="610"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ90946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="645" mass="69872" checksum="59874BD865A8CC04" modified="2017-01-18" version="1">MAPAVGIDLGTTYSCVAHFANDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKNQAAMNPSNTVFDAKRLIGRNFSDEEVQSDMKHFPFKIIEANGKPKIQVEYKGETKSFTPEEISSMILGKMKETAEAYLGSKINDAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKDSGAGEHNVLIFDLGGGTFDVSLLTIEDGIFEVKATAGDTHLGGEDFDNRLVNHFIQEFKRKNKKDLSTNQRSLRRLRTACERAKRTLSSSAQTSIEIDSLYEGVDFYTSITRARFEELCADLFRSTLDPVEKVLRDSKIDKSAVHEIVLVGGSTRIPKVQKLVSDFFNGKEPNRSINPDEAVAYGAAVQAAILTGDTSSKTQDLLLLDVTPLSLGIETLGGAFTKLIPRNSTIPTKKSETFSTAVDNQPGVLIQVFEGERARTKDNNLLGKFDLSGIPPAARGVPQIEVTFDIDANGILNVSAVEKGTGKSEKITITNDKGRLSQEDIDRMVNEAEKFKDDDEKESKRIAAKNQLESMTYSLKNTLSEAGDKLDAADKETLEKKCSEVIEWLDANTTATEEEYTDKQKELQEVSNPIMSKLYAAGGAPGAAPGAGAPGAGAPGAEAEGPTVEEVDDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVX4</accession>
    <name>A0A1E5RVX4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SLS1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g305</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVX4">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RVX4"/>
    <dbReference type="OrthoDB" id="5392646at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048400">
        <property type="entry name" value="SLS1_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20776">
        <property type="entry name" value="SLS1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SLS1 N-terminal" evidence="2">
        <location>
            <begin position="296"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="127"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ90898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="959" mass="110042" checksum="A216CF03424B5202" modified="2017-01-18" version="1">MLRTQIVNRASTAKWNFSRYSSKSLHLLTQPYQKRQYSTGKPTSANLLVEDTGSKNKDHATSALQHDAAAATPELPTSFSPSNVYDDNYYASITNVLKSNMSDTSIKSSLANSSLSPDPSALKSDHISFLSLELKNKEQEKKDLEELEKAMSIVKNTENDLETATKQSNVDANIPEEVDLNAATRGAKKKPAKENIMVINPSQGFGKNRNKRKTYGYLNQKRETLLKTDEYAFIRNNVLNEKTSENSAKELNDFLSTNSSVDHNSITNSFAMNNLNMTMKSIEQMSKKSDGNPTQDKATLLASINSFKPTATTSISPEYHEYLTKTLKKRFTRQQLYDFAISSELSNETSLSKTLAKSSTKNKIVTYILHEIWDIDINYKKVFEHEEILYLDRIQSYLLFFGDENHEIYRYLYERGIKISVELPTVLSKDNEESLRSLVRVQGSKEMCKLAEVYIRERLKKFKTYKFNITDVITNHFGNVDEVTQKLSLKQIAKYIQSEVGCFIQPLVPDVYTECYLHTSSGKDKFTKARRLLLSILNYNPQIHEAVFDFTENPLEEKEINKYPNPNIEPLLWFEKNKVFFRKSLPIKRHASKGSKTEYELTSNQIDEIYNKLDELESTEEKVTISQENLKSNISIQLGSYLEANDKTKTFITQVPHLNEKVLTLPLMVEESESETQQNEKQTNYIQEQSKEALPVTNTIKKTGDEGVTKLHSFEDLETLKIENEKQGVLLENDYETASVSDTTLNSMVVLKFNPKISKNNNYMPSGETQFFKPPPMELWFEKSSNDIKPDIYSARCLLPLNETHIFLKTPQLKFDVKIVKDKYIELCSPXYEEHEEDAPDTLFETADQNNWLSDQQGLKNYLELLSKMKTLNVHNVPRLLTLNIPLKXGVVLENVEYDVVSIKETTFLNFKYMNKYATQFSDLKSPELNSFATQIDFIGGEKLDKNNFGQFLNDVLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RW16</accession>
    <name>A0A1E5RW16_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="17">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AWRI3579_g554</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="function">
        <text evidence="16">This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports the calcium to the vacuole and participates in the control of the cytosolic free calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 17">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="17"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RW16">
        <property type="interactions" value="447"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RW16"/>
    <dbReference type="InParanoid" id="A0A1E5RW16"/>
    <dbReference type="OrthoDB" id="3352408at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02081">
        <property type="entry name" value="P-type_ATPase_Ca_PMCA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000028">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000018">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006408">
        <property type="entry name" value="P-type_ATPase_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01517">
        <property type="entry name" value="ATPase-IIB_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF369">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 17" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 17" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 17" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="387"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="431"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="968"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="991"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1033"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1127"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1160"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="19">
        <location>
            <begin position="84"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="104"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="507"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="572"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="519"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="584"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00059328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OEJ90938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1229" mass="136423" checksum="0D3E7B6CB8E1D224" modified="2017-01-18" version="1">MGADSTKDTLKIPVSVTDANSESGIESVSSNNSFISTDEARNETRSLIHNRHHDNDNISGNQFKLTVDQLSELHDPKSLHAFNNVFDNDYNNLFKYLKTDAKNGINIPKSNDDGDDDYNDQGNNTTNSNLSELEKQRQQVYGINKLPEKQSKTIWQLLWEGLQDRTMIILSAAAVVSLALGLYETFGQPPEYDNEGKVIPKVDWVEGVAILIAILVVISVGAANDYQKEQQFKKLNSKKQDRSITLIRNNGETHVVSIYEVMVGDVISLQTGEIVPADCILISGECECDESALTGESDTIEKYPLKKCLDHFNNDIQDQSLDITNMGNEKILDPMLISGSKLLSGLGTAVVTAVGVNSVHGKTMQALNVDSEVTPLQKRLGKLADNISLFGCLASLVLFIVLFIRFCINISPHHIYHDLPSSEKGSKFMNIFIVSIALIAVAIPEGLPLAVTLALAFSTTRMSKDGNLVRVLSSCETMGSATCICSDKTGTLTENRMTVVKGFINGEEFDDNHDNAIDNSDDENEESGEEAAAMNPQQKKKTQTSISLKKSWSKDLSNDLLTNIVLNSTAFENKDKDDTEENDNSTTENQTENPFDHQLKNKKKHKKRTSSEVVENMVNLNPEPYLGSKTETALLKFADKTLDKKLLHTYRSTPEETELDVQEVVETIPFESSRKWSGIVVKLKSQRDIYRLYVKGASEIVLXECGFVVINEDIIHLDDLQYKDMSEKITYLANQALRTISLAHKDYDLSEISDSSSWPPKFLQNPKNPSTCDPDLLLDPNFDRTNSDNSGMVLDAIVGVQDPLREGVRNSVEQCRRAGVTVRMVTGDNLNTAKAIALNASILTEEQYXDENCAMEGPVFRKLSQEQKLEVIPKLRVLARSSPEDKRVLVDNLRKMNEIVAVTGDGTNDAPALKLADVGFSMGIAGTEVAREASDIILMTDNFSAIVSAIKWGRCVAISIKKFVQFQLTVNFTAVILTFVSAVASKSETSVLTAVQLLWVNLIMDTLAALALATDKPDDAILDRHPKGRGAPLISPAMWKMILIQSSVQIIITFVLHFRGEDFFFNGQATAHQQQQISSLTFNAFVWLQFFNLIVTRKFDEADGIETVKGRFTLNNLNFFSHFFSNYYFIAILLIIGGFQVLIMYVGGAAFSIARQTPGMWATAILCGLGSLLVGVIVRLIPDHWVTKYFPTKLAHKLEYYVFLGFLHKNKEPQFDLENSSGNDGQPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RYZ1</accession>
    <name>A0A1E5RYZ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g246</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000002">
        <property type="protein sequence ID" value="OEJ92161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RYZ1"/>
    <dbReference type="STRING" id="56408.A0A1E5RYZ1"/>
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    <dbReference type="OrthoDB" id="191995at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010640">
        <property type="entry name" value="Low_temperature_requirement_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36840">
        <property type="entry name" value="BLL5714 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36840:SF1">
        <property type="entry name" value="BLL5714 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06772">
        <property type="entry name" value="LtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
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            <begin position="232"/>
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            <begin position="1"/>
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        </location>
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            <begin position="537"/>
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        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
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            <begin position="24"/>
            <end position="53"/>
        </location>
    </feature>
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        <location>
            <begin position="547"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ92161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="597" mass="68353" checksum="1D7CE1E557D25EB8" modified="2017-01-18" version="1">MHSSEKKSPKENTKNALKHLHPLKHPELHHEGGADDHTEAAQKISSREMRAEEGKDEDSDFVEKYGPVKFKWIDPPESAKKRYMIIKPYALNYYHNGVLYRTRGERGSSVFELFFDLLYVGIIANLAESCIEHSDGKSLAKYVLLFFPCWQIWGDMRCFMDYYYNNDIIQKTYVVWIMSLLVTYANNAQTTDENDKQLFGLVVACYMLARFSFATIVLLYNLLYIKEHRRQMVGYCIFVYCSVIMAGFVILPKAMYKKIIILCCLYFWDIVSYAISFSPWFKRFIRSEFHTALNIEHEIKRHSAFVTIALGEFLYVIVAHSPATSGLNERTARAICSLIIAYCLSWFYFMGEGSKKAVHAIRRHAMFSVCWIQLHFPLIISLVIAADAAGELTRSGYDFPMNHAETVVKRATSEGPSLELPRDNYIRDVEIFFGAGIFVALVAMTGIALLDKGLDKEVLWKFTPKVRILPRIMFGGIIFGMAFSDMKITLFLGLSALFLTIQLLFENVEEVDGSFVHEDTDVVKNVINNYDVFDSTEDEQPAQYNSSEEKSHDYEHKETDEDPEIEQEQQGSIESDFASDGGSNASLKQHLQENAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZA2</accession>
    <name>A0A1E5RZA2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZA2"/>
    <dbReference type="InParanoid" id="A0A1E5RZA2"/>
    <dbReference type="OrthoDB" id="3972271at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="747" mass="85533" checksum="75287D5B5E9B3398" modified="2017-01-18" version="1">MKYNPAKTVFDDFSLRDALHVGSVELMKIGFLFLLNICLSVGLYLCSAIKFHTXVAIFVGLIAHHFFNMVYLPAVVVLSXKLNYVSFEGTKQITTKDSGXSFNIFLYLRDWCLLAIFLQALYCLCKKCTIFQNLSNNYHTYHENVFFWKYKNSYATFXSDVLEFFVYIGLIGCISYFVVEICTLKIKDAYELASEAPANYNSDPPLTNHEEYHGYVLCSEERTKPEAGXALEDYPQDFIAHPLTKSVIIHKVYSLQILQVFQNKVDYNIPEKSAVNIPLFSHXNTGSPPPKVVKLEIDEXFGRFLSILYDDGTITIFELIKRQFVAKNYKYSVYSNGTRALESFFKLRSEGSSSSNSSMVSKEQDQDPLVGKVFGXYIIIDSSGIMYTIRFMQENLXPVIGSNITDVSYENQWSINTNSSRISSNKITQCHKMITLRANDRLIFQTDDNLVHVCQEIVNKPWRSRPLIILENAFNTMKKGRTLANPAGGIDNRMYPSGIKILNLSYFGFTFIFNPITMKGELIDVQTGTLMKTFKIDQRYCFDDSLQIYKDVMPTFCKFCGSASIKKLCLTYNIKVSIPNTTKDDPSFSEQKVLMKTHIFKTNKKNKKLICLRVERDPREIRCFGLESVSEKTCDLDVTEKTWFLKKYSVNDPTRIYVLQSGDAVQNGGGKHGEMLSIINTTKFKIQKFDIIKSPLFWDNKFVNDNDIFGXVAENQTMKAYDDDNILLLRKNNITNSSALEIIYNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZP9</accession>
    <name>A0A1E5RZP9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Putative mitochondrial carrier protein PET8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g170</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZP9"/>
    <dbReference type="FunCoup" id="A0A1E5RZP9">
        <property type="interactions" value="759"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RZP9"/>
    <dbReference type="OrthoDB" id="276989at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000018">
        <property type="entry name" value="S-adenosylmethionine mitochondrial carrier protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45667">
        <property type="entry name" value="S-ADENOSYLMETHIONINE MITOCHONDRIAL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="4"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="90"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="197"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ92452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="286" mass="31543" checksum="002187D9E0E0F5E4" modified="2017-01-18" version="1">MNTNDMMISLLSGACAGTSTDLAFFPIDTIKTRLQAKGGFFHNGGYKGIYRGLGSCVIASAPSASLFFVTYDSMKTFLKKKYGKDNDRGNYTLIHMVSSSLGEISACLVRVPAEVIKQKTQTTVGSTSWKTFTSILHSSASWNDLRLNLYRGWSTTIMREIPFTCIQFPLYEYLKAYWKSKEQQSQSPNDNSKHVTLSPLKGAMCGSIAGAIAASLTTPLDVLKTRIMLSTDKQRDSLWTITKNLYKEEGGIKVFFKGVGPRTMWISAGGAIFLGVYETVHAVLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZQ2</accession>
    <name>A0A1E5RZQ2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative serine/threonine-protein kinase IKS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g37</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZQ2">
        <property type="interactions" value="210"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZQ2"/>
    <dbReference type="InParanoid" id="A0A1E5RZQ2"/>
    <dbReference type="OrthoDB" id="1405469at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF138">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE IKS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="159"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ92316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="592" mass="67000" checksum="1CAF51DEEB24A067" modified="2017-01-18" version="1">MSLILYKQVSPPIKRXGKQELALNDGHIILKDPQSRSVVVYNHSSEELTIYQQSSKTDVIEQNQFNSLIGTASPPSPDNQXAFTKNADSCPTCGQRIPNVLDQKTQNDSDQQVFEDYVEPFMHENYFKILNKGLLDSAINTHKGIPSNLFTQGYYKEFFQELGXLGVGARGSVYKVQHVLLNSLSLGIYALKKIPIGDDMKWLTKSLEEVKLLSKITNSHPNLIGYNHVWLEMDKTNGIVTGTDSEFLIPCVFILQQYCAGGNLEDCLVNQVWKLNQSFESATMRKXKHKLRKNSATDSDYQENNTLSNLQIISILRDIANGLVKLHELGIIHRDLKPSNCLLDSEYTGSSTDAFPSVVIGDFGESQFDGQLRSRTGCTGTTEFTAPEVLMTTMDGSSTSEFTFKSDMYSLGMIGFYLIFQKLPWKPYSTLDDLKNMXKKQTFDHESLIAQFGTKENVDPKLFHLLEKLLSHNPDXRFSAKQVVTFLSEINITPIAEDVLNKEKNLEEENFIIEDKETKEGVPPLPXIKKHDYYFKILNVASALFFIINPNRSSVPQINYVLFAVGGYLWNNGQHRYLTGIXLAYLLFLYTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZT9</accession>
    <name>A0A1E5RZT9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative target of rapamycin complex 2 subunit BIT2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g38</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZT9">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZT9"/>
    <dbReference type="InParanoid" id="A0A1E5RZT9"/>
    <dbReference type="OrthoDB" id="2290221at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013745">
        <property type="entry name" value="Bit61/PRR5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08539">
        <property type="entry name" value="HbrB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="154"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="598" mass="68015" checksum="CBD547FD9D0D95F9" modified="2017-01-18" version="1">MGKPKSTFELSSLNPLHKKYSNNKSSTRSRKGSIFSLSSDVVPQTLDVNHQDTTNPLIQSVSESRKVKNRNRSDTSTTVLSNVGFQSIGYNRPSEDYHKNAVEGVRNPRVSVDRYSNAEVKPGTSHSSTPGLYLTSTNISSRRNADLRNSHMLSPISSTRRSFSSSRSSLQRSRSHSSDFDLFLEPAVPHETSSSSSPNLGKKHHFYTKFLPRRKSLSKNXATHRNTNEENAVSSISKMLHYPHYNTKHGHGTFSNGTNSSLFENSASKRQVSTHVLQDLITQNETLTKNDVMYLQDLMKNIPTLELNFHQFSNEERXRILQNTWHVYCNICFALFKNHRIWNLNNKIEDLNRVLQFYIKMVQYNVKKKKHSSKSFASSSEQSRSSGLSSFAEESETPSFGNHPKSLTNSSSVLVNTLNSANKEVISTFLDNLEAFFLSALYVLENQIVFNYKDESVINNPVRRMCIIWKAFYENIFNDVLILNNPFDTYFDKQYNYAGGNKHASTNNAXRSTDIXIENILLRCFRDSIVLPYYQNFSHANEGGSASFMKFINEQDDIKTKEKLLLLQCLGVLKTIGSKDQNQLIIDELSTGLRMSMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZV6</accession>
    <name>A0A1E5RZV6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Structural maintenance of chromosomes protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g15</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMC family. SMC3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZV6">
        <property type="interactions" value="1282"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZV6"/>
    <dbReference type="InParanoid" id="A0A1E5RZV6"/>
    <dbReference type="OrthoDB" id="431497at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03272">
        <property type="entry name" value="ABC_SMC3_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041741">
        <property type="entry name" value="SMC3_ABC_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43977">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="5 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="11">
        <location>
            <begin position="538"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1070"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="179"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="258"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="411"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="693"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1008"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1095"/>
            <end position="1106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ92288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1241" mass="141292" checksum="8CE685F429A213E2" modified="2017-01-18" version="1">MYIKRIAIQGFKTYKNTTLVDNISPHHNVVIGSNGSGKSNFFAAIRFVLSDDYNNLRREERVALIHQGTGSVMSAYVEIVFDNSDKRMHVSSASSFSLNSQYEQDEVVIRRTIGLKKDEYSVNGKSKTKQEIKSLLESAGFSTSNPYYIVPQGRITALTNAKDHERLNLLKDVTGANXFEKKLKDSLANMEETENKRLKISSELAELNFKLGDLNEEKEQLIQCQDLEKQKKVLEYTLYDRELSDVILEIDKFQANXQENVNESTEYVEELEKRETLVREIMEKIQNLNKDLALKESTKVLQLKEQKLESDTEIAKLEIAKNDVQQQLSTERLTTTKQLKLLKHIESRIKSNILQINETEPAYRNLSKKESAIKTEIEFLSQRQRYLINKKGKYAKFSTQSDRDVWIKTHVSELNDSLEALKKDENKNTQEVAALKERLSGIQEQLDDIQDSIDGPDISGKLSDAENSLLALKSQYYEQIDQRKQHWRQEQTLKTVLDSVSTEIEVAERKLTESMNRNLSNGLNAVKKIAKNLNLGDDKIFGTLGELIQFNEKYKTCVEVIGGNALFHVVVDNENTATILMNELHKDKLGRVTFMPLNKLDLSEQISYPSMEENCIPLIKKLHFDPKFSTAVKLIFGRTIVVKELTTGVRLAKQYKLTSITPDGDRVDQKGVLSGGFNDFNQKTTRLDSLKILKQKRNEFAVTERHLAELKAILEQNDTEIDKINSKIKEVTSEKESLSDTIESFKNKKNQLLSSKYGLEEQNKLLSTKLSNLKNSIETFTSKIATLKADLHQPFNTELSNEENLELDSIKKELQDKDSELSLVDEQVNGLSLELEALKSENKSKLEPQMASLKQTSAISANISDLETELVKLEAQLSKVKATGDEITKSLADLKNEINNMTAERANNEKLLEKANSQQRLLLKKIDEFQKDSEKAMMKKATLTARKDELQRRISELGFLPEDAFSKFEAVSTEKCVQQLTKVTKRLSKFTNVNKRAGESFTRFLEKKEDLENKAKDLDLSKKSIEDLIKTLKDQKVHAIETTFEKVSLYFTEMFEKLVPRGTGKLIIHRREEASDVTQNTRSLKKRKRAGNSDTGEELGDPEEDASQADNLYEGVSISVSFNSKHDEQLHVEQLSGGQKTVCAIALILAIQKVDPAPFYLFDEVDAALDKQFRNSVAATIKELSVNAQFICTTFRTDMLQRADRFYKVKFENKLSTVVEVSRDEAIHFIKGDSKNTMAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZX6</accession>
    <name>A0A1E5RZX6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 18">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="6 18">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AWRI3579_g83</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Both L- and D- forms of purine and pyrimidine nucleotides can be used as substrates, but the activity is much lower on pyrimidines. Negatively regulates the biosynthesis of acetyl-CoA from pyruvate in the mitochondrion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 19">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="6 18"/>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZX6"/>
    <dbReference type="FunCoup" id="A0A1E5RZX6">
        <property type="interactions" value="776"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZX6"/>
    <dbReference type="InParanoid" id="A0A1E5RZX6"/>
    <dbReference type="OrthoDB" id="275353at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00318">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000003">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000019">
        <property type="entry name" value="Phosphoglycerate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="entry name" value="Phosphoglycerate kinase, N-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015911">
        <property type="entry name" value="Phosphoglycerate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406:SF0">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00111">
        <property type="entry name" value="PGLYCERATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="11 18 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="24"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="64"/>
            <end position="67"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="372"/>
            <end position="375"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00016471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OEJ92305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="416" mass="44663" checksum="C2EEAA0D26C8620B" modified="2017-01-18" version="1">MSLSNKLSVKDIDCSGKRVFIRVDFNVPLDGEQNITNNQRIVAALPTIEYVLKQNPKYIVLASHLGRPNGEPVAKYSLKPVAAELSKLLKKDVEFLPDCVGEQVTKATSAAKNGQVFLLENLRYHIEEEGSRKVNGEKVKADKKDVDAFRAALTSLADVYVNDAFGTAHRAHSSMVGFQLDTRASGFLMQKELEYFGKALENPDRPFLAILGGAKVADKIQLIDNLLDKVNTIIVGGGMAFTFKKVMENSEIGKSIFDAKGAEIVPKLVEKAKKNNVQIVLPVDYVIADDFSATANSKIVTDSEGIPADWEGLDAGPKSRELFDKTIKSAKTIVWNGPPGVFEFEKFAEGSKAMLKSCVAACESGATVIVGGGDTATVAKKFGGAEKLSHISTGGGASLELLEGKALPGVVALSDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZY2</accession>
    <name>A0A1E5RZY2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative kynurenine--oxoglutarate transaminase BNA3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g39</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZY2"/>
    <dbReference type="FunCoup" id="A0A1E5RZY2">
        <property type="interactions" value="430"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZY2"/>
    <dbReference type="InParanoid" id="A0A1E5RZY2"/>
    <dbReference type="OrthoDB" id="2414662at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016212">
        <property type="term" value="F:kynurenine-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000024">
        <property type="entry name" value="Kynurenine--oxoglutarate transaminase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051326">
        <property type="entry name" value="Kynurenine-oxoglutarate_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43807">
        <property type="entry name" value="FI04487P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43807:SF20">
        <property type="entry name" value="FI04487P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="6">
        <location>
            <begin position="41"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="7">
        <location>
            <position position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ92318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="435" mass="49019" checksum="4D769FDD21CB8A29" modified="2017-01-18" version="1">MASNFKFTPNKKFLNTPKDVWSFTNETAAVASTNANNKGRELINLGQGFFSYAPPEFAIEAAKKALDPASNNQYAPTKGKPQLIQALIKQYSKYYGENALDAENIQVCTGANEAILSCLIGLINEGDEVIVFEPFFDQYISNIQIPGGVVKYVPINPPKNLKTEITTGDDWTIDYEQLENTITEKTKVLILNTPNNPIGKVFNKEELTKIGDLCVKHNIVIISDEVYEHLYFNKKLGFPRIATLSPEFAQLTLSVGSAGKSFAATGWRIGWIVSKNKELLSFASMAHTRVCFSSPAPLQVACAESIELALHNDYYEDMRQQYIKKFDILCSAFEYLGLPYTKPDGTYFVLADFSKLKIPFDTYPFPQEVKSKAKDFQIAYWLINEFGIVAIPPTEFYIPEHEQAAETLLRFAVCKTDEYLEKAVDRLKLLKDYIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZZ3</accession>
    <name>A0A1E5RZZ3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Bud site selection protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g172</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZZ3">
        <property type="interactions" value="161"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZZ3"/>
    <dbReference type="InParanoid" id="A0A1E5RZZ3"/>
    <dbReference type="OrthoDB" id="4066896at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057454">
        <property type="entry name" value="Bud3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021895">
        <property type="entry name" value="Bud3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12015">
        <property type="entry name" value="Bud3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25351">
        <property type="entry name" value="PH_BUD3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="2">
        <location>
            <begin position="329"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="856"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="985"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1186"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1237"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1416"/>
            <end position="1464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1786"/>
            <end position="1840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="856"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="897"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1006"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1016"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1090"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1186"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1201"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1240"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1268"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1416"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1805"/>
            <end position="1820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1823"/>
            <end position="1838"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ92454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1856" mass="207639" checksum="007C3BD6D431F108" modified="2017-01-18" version="1">MRLTFAGDGNAASSTGDFAIKSVVKTEQDNSEIFDDSVYSTHSESNDTPVHQSEETIKIRSKETLQHPETSSIATEKIIFEYQDTQKDSLDSTNSMKLPANLFDVLLPDDKLIAENFEEIFNSAVFIRSNDPKVFGNFIAIVYNISGKPDTFCCYLFDKFGCTYHENVDISPVSKYYHAIENLSEANKKLNVPKVVAVALLQKYAYAQKFNPDFFPKILAFDPTDAGMALNEHKLVETNSKLTPLQLGLKMQQMSCLQSRIIKSLFIDVLYENNSDVIDDNNKLVFHLGSQLEQLFNPLTEYSPEQTEIVYKPVPPADGSSLVLKDSSLVQSIIKEFVDIQTIFTVELVNFLQKYIIPIRVKVSNNEIPGISFSKLNKLFPPTIDEVTRINCIFLDALKLAQNYGSLQILKACSVTIAYFYKAYTRHEAATKKFHKDIKLFLAKFNDQLPLNQVYTELKLETIITGPQEKITKYKLILQRLWKEKRWETESAEQEATKYYNNIIDVIDTFGNQATPSSKMSAYQNRVFTPSGKILTELAEKWPIELQYKWLKRRVVGVFDGVHYTDNKRDVVVIFNDYVVFLNIIDGDLYYSEGSNKPLISDILMNSLINEVPLPNNIPQLTVRKHAHINQLLVTSFGDSGKQIRFDVLDGKSFPLYYQLVXPTDTCEYICELIVKAGILCKSTAFHLFNKSFETNSVDSXXXQSSKLKTYVTAHEINTYKDEKIKSPFAIFLNIDPSYATLKENDVYFGAFLKFVDASKKLVQLQILTAKKENNQGFQLSKTEFDESPIIVSLSEYTKVILSLLAKYHLKEYYYSLLSPLTDQIFSINEHVVEQVQEPVKFFTRGLVKSENLQNLPTVSSPTTETLNHMDKSVTSQSTSVGESTPHNSSFMDKSKSNSTITTFSSSSNQLXKNPPLFQKNGAAPXAXIRHASTVITKPVKQSAVPXKGQXTKXXVVAGNDNIQKQVIKPPSKMANAINKKEKVVLQQAPKAKGAASKDLSTKEKSKGKKKKNRLSKVFASIFSSSSNPTKPDSSLKAPAAASTKAAKRQSLPVIPSPSXDIKGSAKSELPSTKVSDKTPAINKKRFSLGSITTPSSATPAFKNTDNTALSKGTTGSSNSGQSKVTLSPLKTPGTRKYSSSNFSGISKTPISSPLKISRDHLDANMEIPKMQSNIEQDKMFSNPXLXVXKSPLSLNQNTEKQTEPSKKQTTEAVFNDDLYGEILNHDEKYFVKAPAKATNVEQPNPNQARSAVRNEQKXVPAPEQKRSSSSIENDLTNTXTSSGSHXETEGRKLKSVFDLPEQSTYGQRKSTGEMVFEKLQAMDESKPTSVFAPLXSEHQNPSETFRKNVNFPQSASYRELFQDMEKVLNPHEKNWNWVSLEKSASLENVETAAAKDGNSREQLETDLLEKYYEKIPVEEKDSSHKDGDVEVKENPQYEENNNREEKAHSLHDTENFDSPQGNQNFAESVEENLGCQHGNQMNEKDKNIFVSEAPASNVANVNDMNVSRGADDIACEINELNAKKQNNFSAPSNNGQLLDDPQRVRMRVNYQSKFRVVKSSSPTRAPLDSAVSXSTRPSQKLXLDETIPVLAATYSRSTATDSELDFRSDSKAKVNARFFPAGSESSELSSMSVSPRKANSGIFNRLLDLQDHIAEQPSSRDISFHTSDLEKDLGESTLEFSDFDITFNRHDTLDEKTEEEFTTPKNVLLQSAVSPEIEEPIFYDFNTTQDYSNGVTNSSSKKGMDFSNNAIWVSPSKLDVFDLSKKSPSMYERLNVAETNKTGKLVFHDAPDNPNSELGATSYDPKRNDDLGHQAEKESSINNNNNNTQPNTLVNKNQNGKVLRDESYGYLSGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S002</accession>
    <name>A0A1E5S002_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">G1/S-specific cyclin CLN3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g213</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5S002">
        <property type="interactions" value="221"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S002"/>
    <dbReference type="InParanoid" id="A0A1E5S002"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044772">
        <property type="term" value="P:mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048258">
        <property type="entry name" value="Cyclins_cyclin-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00292">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 4" id="KW-0195">Cyclin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="78"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="399"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ92338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="462" mass="52342" checksum="5B5A494D3DA148C5" modified="2017-01-18" version="1">MPNRYTVMSMEIFSHEQACQEYGVEFLQHLLALDKKYDVQVKHTNANYGSPASMVYSSQSIENIMFLNYYSLRKLIFDFMMCCHTKLRLSTPTLILSFQILDKYVTLLRLEQEMLLDRLKFQMIGVVSLWIASKFMDPKIKHPDINILLQLISYNKSPPGGAATSNDFAQAKEKVKMETSGKLKSLELDILKHLNWCVSDIPTHDFFVDILLRSIETFDDTQQEKRFENKTQSCFSMEDINQIKYGAQMLCELACFYPHINNNHSVSSIAYSSVILIQKCMQDFYSCGEKNXFSTELSLDGISYKLLEMFKDCHNALNEDGLFALPFSFKLKYFPKNASQNPLFLQSLLNFVSSQFYSSLGDSXVLQKQDTRDAAAAAVGSTPSGAPYLSAKAIPPTPNTPSLLDLGKPVKPSATDQTGPRPSTRGKRSLYELDPAIEEGAEQTFNPGKKKLHQELFIESTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S003</accession>
    <name>A0A1E5S003_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Dual specificity protein phosphatase PPS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g48</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5S003">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S003"/>
    <dbReference type="InParanoid" id="A0A1E5S003"/>
    <dbReference type="OrthoDB" id="273181at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008138">
        <property type="term" value="F:protein tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033260">
        <property type="term" value="P:nuclear DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000110">
        <property type="entry name" value="PPS1p Protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053239">
        <property type="entry name" value="Dual_spec_PTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47550">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE PPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47550:SF1">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE PPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="4">
        <location>
            <begin position="705"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="5">
        <location>
            <begin position="779"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="59"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ92464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="903" mass="103571" checksum="E319916939CBE148" modified="2017-01-18" version="1">MLNNTSVVRNKETQIGCCNVVEEHDTXEYSNNSSADELPLRISKKSKISTNEDLELSETKAQQQQQSTQETSPVXKYQRISLDMAYEFLQNNMNTPLPEGNVMFPWLHSTNTTPLENNEVISSNCKTDTQTQNNPINNTNCKKFIKNNISVIRSAPPGLFESNNGDKKETLYIENSGILHNSVDLRDLFVPFDLYCHDLYAILENEYTEFIQKKRKEDGFLKKDESFSINAKEIEELCEYCSKNRILPILNVDDVGQCLYGIRKRRLSKGTHAMSHHTTRDPAHEPQLFRRFDVQCGKMLQMSSKIIVYCFNCKSHKNDLGSSTCFCTTLLKIMQMSLERNFERSGYIFPLKREFYIVDVPKQSVNYEHPMIGTPLMLCESLXKRETQLVSEFDATILNNWEKDLFYREKLEVSKISSASVINELGNVWCGNSIDYEISKMPEKLGNCSLHGRKNDYFTLQDSICGIDKLKXKNYKENDTLLFNLPQVKWSYFIHCTNCFQTDGGSITENIMNDTMYDSILQDHHHCQTAHKNNLSSGFNXYKNNNTTLKKKLNFPASGSLGLGNLTLASIKMIFQFVDLIYQLSLPADQNKENHNNACGDSQILIYSTDGYTETSLLVVAYTIYAQNLTLNDALTYLHIVHKRPFFLFPSDLQILFYLQNLLLDKSPQNPNRSTSKEITNEAFTTMFFSTDVTKNSLMTLKGPLPSRILDYLYLGSLKHAENYELLKNMGINCIISVGEKLNWIQSYQTPKTQIIKEKGFTVFHIDGLADNGKDPLLHSLSSILNFIEEQRLKGNKILIHCMVGVSRSATVVIAECMRYLKLDLLRAYIYVRVRRLNIIIQPNLMFMYELLKYEELLNAENSLNSVSDNLNGYKLKGKPIRSINWQILCKAVNELNGNYLGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R030</accession>
    <name>A0A1E5R030_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Polyphosphoinositide phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g4657</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000017">
        <property type="protein sequence ID" value="OEJ80247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R030">
        <property type="interactions" value="772"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R030"/>
    <dbReference type="InParanoid" id="A0A1E5R030"/>
    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043813">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043573">
        <property type="entry name" value="Fig4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45738">
        <property type="entry name" value="POLYPHOSPHOINOSITIDE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45738:SF5">
        <property type="entry name" value="POLYPHOSPHOINOSITIDE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC" evidence="5">
        <location>
            <begin position="169"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="717"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="717"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="6">
        <location>
            <position position="772"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ80247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="808" mass="93857" checksum="424627605A374605" modified="2017-01-18" version="1">MKEXSHTVNKTKSKTRGMPRTQRKNTRFILTKYTIYETEKRMYIVGSNKRENMFRILEIDLTVPQDQLVVLEDNIFFTRDEVMKILQEIDKEDENDTFSKKLTADGLLGFIRFTECYYLVVVTKKSQVAVLGGHSVFHIDQTELIPIVASNANKKMSKTSIEARFLATFDNLNLSKTFYFSYTYDITNTLQTNLLREKLKGVXRSDIDIPSGSGNSYNEMFVWNQNLLGPVLEYIDTVYDWFQPIIHGFIDQVKISVFGRPIYLTLIARRSHHFAGARYLKRGVNNMGYVANEVETEQIVTDMLQTSFHDNERRFYGSDRYTSFVQHRGSIPLFWSQELSNLTTKPPIELNVVDPFFSCAALHFDKLFQRYGGGKIQILNLIKKKEKTARESILGKEFTNCVEYLNQFLPEQNKLDYTAWDMSRASKSRGQGVIEFLERYAINSLETTGIFHNGPQLSDTELQQGVCRSNCIDCLDRTNAAQFVIGKRALGYQLKALGIIDDTFLEYDSNLVNILTELFHDHGDTIALQYGGSHLVNTVQTYRKINQWSSHSRDMIESIRRFYSNSFYDNARQDAVNLFLGHYVWKEGQPAIWDMGTDFYLHNQEMYYDFEKPKRSYCQWWNIHNIRNISNLIETEVVSAGNDLTQEKIVKNVREYPKTVDNYWNEYYSIRTYTSVKGMFELKMNNTDRYTRKKDISPFVGNRRAGRVTVEXKSVETDENFETHAKPGQDSVSHGDAHAQSGTSEFKAFVDEETTXLYQDYSRINVGNSLDDYPSLSFDEKDEKDLFDYYTDYVKIPIKDEQGSVLIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R097</accession>
    <name>A0A1E5R097_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Pre-mRNA-splicing factor PRP9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g4481</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF3A3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R097"/>
    <dbReference type="FunCoup" id="A0A1E5R097">
        <property type="interactions" value="1270"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R097"/>
    <dbReference type="InParanoid" id="A0A1E5R097"/>
    <dbReference type="OrthoDB" id="2160351at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000690">
        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031590">
        <property type="entry name" value="PRP9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031774">
        <property type="entry name" value="SF3A3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024598">
        <property type="entry name" value="SF3a60/Prp9_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16958">
        <property type="entry name" value="PRP9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16837">
        <property type="entry name" value="SF3A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11931">
        <property type="entry name" value="SF3a60_Prp9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50171">
        <property type="entry name" value="ZF_MATRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Matrin-type" evidence="8">
        <location>
            <begin position="303"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="405"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="413"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="566" mass="66125" checksum="4C0ED08381D0D3CF" modified="2017-01-18" version="1">MNDIEQERLNCEKLDTIALNITRRFERNPAVFYRNQAEYYKTGFPTTLASSFDNSELARKNSLLKVEKPLRSKKQILAQQHEINRFCKIFKDTKLHMQEGKGFTRDLVQVAKGELGNQDELDFLRFDSIVNNIIDEHESRTGNSEKTHFIKSTKADSLDDEFSMFPHQLQILSKAAQDININSFFQREEKYGEFLNLIKYHTLWLSVIKSADLSYLEFLRITIGNFKKQDYAYILSNKKWRDTPQYHYFLLQLNNYLVHFIQKTFPLFPYEKFEEHRIVARFDNFLQTGLNIDSQEHKSENNIYCLVCDEFLHTEASPNNASQKSHGQTKKHIQNLKKRENVCFQELKLHMLCSGLLSEIIDNTESFTEMKLSYTNKERLLELDKLSQLYEEDLYTQKEFELDQQLKSGNPRSRAEKDVSTNRKSTEYGHVDPKGSVSKEDQSTAEIPKWLRKLKKLDVEYQCEICGNVTYKGYKLFQKHFFESKHVLGLKCLGYSGNPSQFKGIVKVSDMKALQYKLLQTASLVDRTDATQGANTSKDATKSELESNQWFVEVEDSQGNVVKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0A2</accession>
    <name>A0A1E5R0A2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g4552</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0A2"/>
    <dbReference type="FunCoup" id="A0A1E5R0A2">
        <property type="interactions" value="561"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0A2"/>
    <dbReference type="InParanoid" id="A0A1E5R0A2"/>
    <dbReference type="OrthoDB" id="996720at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002673">
        <property type="entry name" value="Ribosomal_eL29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12884">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12884:SF0">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01779">
        <property type="entry name" value="Ribosomal_L29e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="22"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ80321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="85" mass="9876" checksum="1B5EE0D668B39F1C" modified="2017-01-18" version="1">MTKVFNPFDSIVLITKQFHQQKMAKSKNHTAHNQTKKAHRHGIKRVKTHKYSSLKNVDSKFRRNHRYALHGTAKALAARKAEEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0H6</accession>
    <name>A0A1E5R0H6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g4530</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0H6"/>
    <dbReference type="FunCoup" id="A0A1E5R0H6">
        <property type="interactions" value="43"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0H6"/>
    <dbReference type="InParanoid" id="A0A1E5R0H6"/>
    <dbReference type="OrthoDB" id="292213at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052241">
        <property type="entry name" value="SLC66/Scramblase_ANY1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14856:SF9">
        <property type="entry name" value="PQ-LOOP REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14856">
        <property type="entry name" value="PQ-LOOP REPEAT-CONTAINING PROTEIN 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="301"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ80401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="359" mass="42060" checksum="097BFEFA04615700" modified="2017-01-18" version="1">MSETQGQDVGQVVDDGSFLNLGGYEQYLPKIDQFYIPEWFSMQFVINNMISFTPLISYGSTVLSIRKAQTAMGFSIDICATMLIASILRVSYYLITPYEVTLLRQALIMIFIQVILLYTSLKYRPEDYIYENLDDVESLGELLHDVWLEYFPANPFSRKEMRHLIKSLSWKNLLRFAYKMALVAVYKFLKFFDPSYKRFEKFWQWSEKKKFWQFICVFVTVQIVITFFISKVMNWESFAGWIGSFIGSLGLFIESTLPLPQIAILSKLKSVQGFKLILLVSWLCGDILKICYLVFGANNISVIFLFFGLFQMGLDIYIGVQYVYYKYYYTGRTYFDENPTPAVYRPHSSKEDVEMTEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0H8</accession>
    <name>A0A1E5R0H8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4447</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDPK1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0H8">
        <property type="interactions" value="354"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0H8"/>
    <dbReference type="InParanoid" id="A0A1E5R0H8"/>
    <dbReference type="OrthoDB" id="3972123at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000196">
        <property type="term" value="P:cell integrity MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010606">
        <property type="term" value="P:positive regulation of cytoplasmic mRNA processing body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060211">
        <property type="term" value="P:regulation of nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05581">
        <property type="entry name" value="STKc_PDK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000191">
        <property type="entry name" value="3-phosphoinositide-dependent protein kinase 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000534">
        <property type="entry name" value="Serine/threonine-protein kinase PKH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039046">
        <property type="entry name" value="PDPK1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF163">
        <property type="entry name" value="3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="163"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="54"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="478"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="532"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="902"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1070"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="70"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="478"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="537"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="605"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="646"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="942"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="965"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="982"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1070"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="13">
        <location>
            <position position="450"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="13">
        <location>
            <position position="960"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ80408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1101" mass="121849" checksum="9547F0751B78A822" modified="2017-01-18" version="1">MTDYTNIASYGGETKIRHXSQSHDRVSNDVLENSAYSLDPGRPPVPKAINYAITDSSSQSDARRGKKVQSHVSSRPPSSLSEASGPSRNTTPHGNNPTLDAYSSSALDPTRELSSSEVPVSVEFQKRRXEWAERGAAKLVREYQDPDTGETKKKIVSQGIKDFKFGQEIGDGSYSTVVLATSIQDPSKKYAVKVLNKAYLVRQKKVKYVNIEKQALQRLNNSKCVVKLFFTFQDENSLYFLLEYASNGDFLSLMKKYGSLSEECTQYYSAQIIDAIGFLHKRGIIHRDIKPENILLDTSFKVKLTDFGTAKVLDSLPNGEHDDGLEEPAAGGKKYDLLTRSKSFVGTAEYVSPELLNDSYVDYKCDIWAYGCIVYQMIAGKPPFKATNEYLTFQKVMKVQYAFTAGFPLVVRDLVKKILLKAPEKRLEIIDIKKHYFFKGLNFSKIWDLDPPPVAPYKMSAKSMSPIPALSHGNYSASPKPLLTKTPKKASTPTIHTTKVHAPLVQSSPLSKVAAKKSAVDPKTREILESAKRSVASKKQQQLRESQSKTNLGLGLSSAQNPKNAAVAAVAALSKTSSPSQKPRRKAPSSKQIGAAKEIQHVTPSRSSSSLSSVLNYDYSSDVTKATTDGSHDSRKSNGNTEMLRSPSSSNFSPSHSIISTPDSPQGAVAKSKTQSSWAPYMRSNETVIKMGEIDMVSLPSQVLEKVLHKKSLTLKAIPLNDDQTGYNRYSTGKTSFLSQVAKSGLTGFRDVEEHDLYIEEKMRSNFEPAPEYALHHLDYRILDSDHSSLRKIFSVYSEEEEEELHDKISVVPVEYYERKLALITSQGRCLIFIKSXTSVYHLQYRLYLNQAGMQMKELTSTFNNLKENYHFVLESPFKSFVFQCSIGSSVSWITCLQESMASSKKPSRRPPPLASSTNRVSSNPPSPALASASSFARTDKNSSAMKDISSTKXSNSTVDXALRSPLXXTPSSTTAIKTAKAKKDPSTKQKGERMFDSYVTMKQKKHDSSSKPLKPIQNPIKLVNGLPVLSRPSNSVNADATDLHSYNSEQQHYEDGIRKEIVNAAELAASRRASSGSSEHTSAGLSKLDNMKKKLLSRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0I2</accession>
    <name>A0A1E5R0I2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">3,4-dihydroxy-2-butanone 4-phosphate synthase</fullName>
            <shortName evidence="12">DHBP synthase</shortName>
            <ecNumber evidence="3 12">4.1.99.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4444</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-ribulose 5-phosphate = (2S)-2-hydroxy-3-oxobutyl phosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18457"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="4.1.99.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="12">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="12">Binds 2 divalent metal cations per subunit. Magnesium or manganese.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 12">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">Belongs to the DHBP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.12" evidence="3 12"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0I2">
        <property type="interactions" value="124"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0I2"/>
    <dbReference type="InParanoid" id="A0A1E5R0I2"/>
    <dbReference type="OrthoDB" id="60371at2759"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00399"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008686">
        <property type="term" value="F:3,4-dihydroxy-2-butanone-4-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.870.10:FF:000002">
        <property type="entry name" value="3,4-dihydroxy-2-butanone 4-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.10">
        <property type="entry name" value="DHBP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000422">
        <property type="entry name" value="DHBP_synthase_RibB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00506">
        <property type="entry name" value="ribB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327:SF18">
        <property type="entry name" value="3,4-DIHYDROXY-2-BUTANONE 4-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327">
        <property type="entry name" value="GTP CYCLOHYDROLASE II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00926">
        <property type="entry name" value="DHBP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0318">Glutathionylation</keyword>
    <keyword evidence="10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 12" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0686">Riboflavin biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ80409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="214" mass="23607" checksum="0925CCA7F0D0C92F" modified="2017-01-18" version="1">MSNKANFSPIEEAIEDFKQNKFLIVMDDESRENEGDLIIPAHNITSQQMAFIVKYTSGYICAPMTNSRADKLDLPLMSTLECAGIHDKKSTAYTMTVDAAKGTTTGISSSDRSLTCRNLADFVNINKPTDFIKPGHIVPLRAVDGGVVKRGGHTEAAVDLCRLSGLPEVGVIGELVREDDGEMMRLKECVEFSEKHNIKIITVEQLISYIKXQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0T3</accession>
    <name>A0A1E5R0T3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Repressor of RNA polymerase III transcription MAF1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4502</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0T3"/>
    <dbReference type="FunCoup" id="A0A1E5R0T3">
        <property type="interactions" value="114"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0T3"/>
    <dbReference type="InParanoid" id="A0A1E5R0T3"/>
    <dbReference type="OrthoDB" id="277029at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000994">
        <property type="term" value="F:RNA polymerase III core binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016480">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.50">
        <property type="entry name" value="Repressor of RNA polymerase III transcription Maf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015257">
        <property type="entry name" value="Maf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038564">
        <property type="entry name" value="Maf1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22504">
        <property type="entry name" value="REPRESSOR OF RNA POLYMERASE III TRANSCRIPTION MAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22504:SF0">
        <property type="entry name" value="REPRESSOR OF RNA POLYMERASE III TRANSCRIPTION MAF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09174">
        <property type="entry name" value="Maf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="419"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="114"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="142"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="449" mass="50771" checksum="66462565564735B6" modified="2017-01-18" version="1">MKFFDELDIEKVNQQLNFETSDCKVTGGCDLFTTKAAGSDRKLYKTIDKHLDTLVQENEAFKKFLEQHHAEVNNSENGAQAQTYGNEGSPKFTHTEFENDNSLKSGYRVKKSSRSNSSASLSSFWEQKRRMSASESYHNHSSHNNNNNNSNSNNNSNNDNFNQIANSSGKIPMTESSVAENERNPIATGESSGSNSEFLRPSSNKLNEQNLEKLIDESYTGPHPLKKKRSCSSTSSANKMDYIAESSPSALNFNMGPFGPLNENHSRKIFSYLIGIMNASYPDHDFSSLAPTDFSSCSLKTLISKFENSLISLGKQPQEWIWDTINSHMDLSDCAIYQYNPIKTFMDDEIPGHLWSLMWFLFNKKRKRVAYLYLSVARLNQSGSNLLQHGGHGRRLTIEHHNMNSFEGEYDLTYDENAIEDDDENAIEDDEEISIEEAENTPQLNEVAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0V0</accession>
    <name>A0A1E5R0V0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Carboxypeptidase</fullName>
            <ecNumber evidence="14">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g4603</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Preferential release of a C-terminal arginine or lysine residue.</text>
            <dbReference type="EC" id="3.4.16.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="14"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0V0">
        <property type="interactions" value="119"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0V0"/>
    <dbReference type="InParanoid" id="A0A1E5R0V0"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF190">
        <property type="entry name" value="PHEROMONE-PROCESSING CARBOXYPEPTIDASE KEX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4 14 17" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0732">Signal</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009028091" description="Carboxypeptidase" evidence="14">
        <location>
            <begin position="24"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="614"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="522"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="695"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="522"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="538"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="704"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ80535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="717" mass="82675" checksum="7DF8F6DCD6E70653" modified="2017-01-18" version="1">MNTFQTCTLLLPCLAVFASGAPAAEDYKIVNELLPGYSEIPEYVGSPELYTKPTEMYSGRISVDNEEEEEEEEGAKKFFFWKFNTVNGSKDKLVIWINGGPGCSSMDGALLENGPFRVNSGGNLIANNGSWHMYSDVLYIDQPLGTGFSYKVDEQSSEGEGGASLXDVEFYDADIQNDLNAHFVNFLLNYFKIFPQDLEKEIILSGESYAGQYIIHFVDYMMKYNEESSTNFFNVKKILLGNAWVDPMEQGLSYLSFMKQENLIDSQNSKLKDLMHQQEVCQNTINSDFDQLLDNFSIDPCESILFSAIGSAVDEDFKCLNVYDYRLVDDYPSCGMTWPFDLPNVTKFFSKKGVMETLHLDESHRWQECNAMVANYLKNKHSTPAIRILPDVLSNGIQVYLFNGDKDLICNSLGVWNYAKKLVWNDEKFSVNETTNKHVWYHDMEESGYFYKDRNLVFLNIXNASHMVPVDKPLENRGMLTLFDLVSHFDLDYNEFDVYTSIKRSIDDEDYDDVESNYIHYVIKDGETEGTEGTKETESEEDEFDDEKQEQEFDEDESEDDSDYESDEENNEEEGFEEQPEDYENEEEGYKDEEEEEELYDDEEEEQHRQTKHALSVFFLYVVIIGLGGFIARYIIKTLYSKKKDRFNRLDAEDSLDFDDEGLVVPAKKKKTVSWADDIENVDLGNHQEDFEMTDTFDLDDELESGKNTHESTPDKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1I2</accession>
    <name>A0A1E5R1I2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytoplasmic tRNA 2-thiolation protein 1</fullName>
            <ecNumber evidence="6">2.7.7.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Cytoplasmic tRNA adenylyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">NCS6</name>
        <name evidence="6" type="synonym">CTU1</name>
        <name evidence="10" type="ORF">AWRI3579_g4330</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1I2"/>
    <dbReference type="FunCoup" id="A0A1E5R1I2">
        <property type="interactions" value="462"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1I2"/>
    <dbReference type="InParanoid" id="A0A1E5R1I2"/>
    <dbReference type="OrthoDB" id="198857at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002144">
        <property type="term" value="C:cytosolic tRNA wobble base thiouridylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01713">
        <property type="entry name" value="CTU1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000188">
        <property type="entry name" value="Cytoplasmic tRNA 2-thiolation protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03053">
        <property type="entry name" value="CTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056369">
        <property type="entry name" value="CTU1-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032442">
        <property type="entry name" value="CTU1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000541">
        <property type="entry name" value="Ncs6/Tuc1/Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020554">
        <property type="entry name" value="UPF0021_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807">
        <property type="entry name" value="ATPASES OF THE PP SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807:SF12">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16503">
        <property type="entry name" value="zn-ribbon_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01263">
        <property type="entry name" value="UPF0021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2 6" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal" evidence="8">
        <location>
            <begin position="69"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Cytoplasmic tRNA 2-thiolation protein 1 C-terminal" evidence="9">
        <location>
            <begin position="330"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="72"/>
            <end position="74"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR004976-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="383" mass="43262" checksum="5C462745C17512D5" modified="2017-01-18" version="1">MYQPPSDPVNKAKHLKTSQLCELCHSRKAVIKRPKNLMKICKECFYYVFETEIHNTIVSNNLFYKGERVAVGASGGKDSTVLAYVLKLLNERHQYGIEIVLLSIDEGIVGYRDDSLATVKRNQKQYELPLEIVSYRDLYNWTMDEIVACAGVRNSCTYCGVFRRQALDRGANKLNIAHVVTGHNADDMAETVLMNILRGDVARLEKSTAIMTISAGSPVKRSKPFKYSYQKEIVLYAHYKKLDYFSTECTYAPEAFRGTARELMKNLEAIRPSCIIDIIHSGENLVLKPKKQKKQTVKSKNVKKTELNTQEQELEVRTDGTVSLNFKDGNRCERCGYLSSNKICKACTLLEGLAANRAKVQLDLDTSTDGAAKVVRDLEKLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1U1</accession>
    <name>A0A1E5R1U1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative glycosyl hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4230</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1U1"/>
    <dbReference type="FunCoup" id="A0A1E5R1U1">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1U1"/>
    <dbReference type="InParanoid" id="A0A1E5R1U1"/>
    <dbReference type="OrthoDB" id="1887033at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046557">
        <property type="term" value="F:glucan endo-1,6-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050386">
        <property type="entry name" value="Glycosyl_hydrolase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297:SF43">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297">
        <property type="entry name" value="GLUCAN ENDO-1,6-BETA-GLUCOSIDASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="7">
        <location>
            <begin position="119"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="8">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="527" mass="61152" checksum="F7E5255B53D32C35" modified="2017-01-18" version="1">MFDKLKQKILHKDKDKDTDTDTNKNKGKDTNQTPFESPHMENLSLNDSISTAQLYQSRAFHGVNFGGLFLLEKWLYPSMFPDETNGTEYEFDAIAAIASKKGVSEAVKMLEDHYAKFLNNNNEYEWLEQHGIDSVRIPVGYWHVSGGDEKYEMKGMKFAQVKEVYQQYDPLTKLKEVVKNLQDHNIGCIIDIHGLPGSANGQHHNGEEKDNKGGFFGSNFEKYEKYIVDHCIPVLAKEFASFENVIGLQVINENEFVNKDDDRKKFSHVTQYYKDCLHKLRSLNVSWPLIISDGWWCGQWDEWVTKENLQLDVIIDTHCYRCFSDADKSQKVQDIVAKFQCLDSQCSNDFIVGEFSCVVDGASWSLSNANRDEWVKNYGLKQVDTFNQSENIAGWFFWTFKFEYGDGGEWGLKPMMDKGCIKGAPNNSNKKDIDSSMLDRFYKEHASYWDQHPSSDYEHFRFKDGLEQAVADIKMFHNFNGSSLGRVNYWVTSRKTQYCEKQKISPNHKSLWEYEHGYKKAIQEFNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R280</accession>
    <name>A0A1E5R280_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Aspartic proteinase yapsin-7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4125</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R280">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R280"/>
    <dbReference type="InParanoid" id="A0A1E5R280"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47965:SF105">
        <property type="entry name" value="ASPARTIC PROTEINASE YAPSIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47965">
        <property type="entry name" value="ASPARTYL PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184566" evidence="3">
        <location>
            <begin position="20"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="4">
        <location>
            <begin position="79"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="24"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="518"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="621"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="621"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="5">
        <location>
            <position position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ81011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="742" mass="80469" checksum="F6E4ABAF4C8579DD" modified="2017-01-18" version="1">MHSFAHILGISLLASLLHAATLEEKEDEQSSSSSSSSSSSSTESSSSTSVSTSALPPTTKLPSEYPPMLRIGRDESSLYYVNLTLGVPQQKQQLRLDLAQPYLWVSNGDVDSDCNKRGSTCLDTGYYYPNQSYTGTLWDPEYAPFYNMTFIDAVFFNGQAYNDELSFDSNIYYYNSTTANSLTISNMSFFSANYSENLIGALGLSGKTNTSDWGYSDISQYNFDDSFYFLHQMVDQGLINCSSYSLWLGADSLTFRDISSAGISVQDDNVGKLLLGAVDPSLYTGDLIKFKNIAFYNASXETYSYGYPIIPLTGVNVISNTSRTVNLTDSTFLEPVLLDSRYTYNYLPLTLVTELAIQTNAYYVQELSSWYVDCSLGNIGAHVSFXFGNLHISIPLIDLMGNSFTSATGRTLYFSGGNEACVLKVRPSYRVGFSVLGGPFLRNIYMAADLEDNSVAIAQAVIIXDYSAENITSTELLYEYYEEELEEELLSLSSVSAASSSAASKSRAVAKFTTSSFHSSSTSSVNASSKSTXSSKSTSSNXYGISTIVSGSIPYAKTNNVTTEIYFTSFSASSFTTQYGSNILEIFGYNITYASLFSGNEIVTVTRSFYDTTIAIQTSSSLSPSSTLSGVSSTGNSGQEHSSSCSGCSTLVLFTNPTGHYSSSGSGSNRIIDVVTQTFIYVTNSTTYDSKQTVTYVYTGSSYPSDWTGNTNAGSTYGAALSKPLHSGLKLLFFTILGILLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2A1</accession>
    <name>A0A1E5R2A1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein BFR2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3943</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AATF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2A1">
        <property type="interactions" value="970"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2A1"/>
    <dbReference type="InParanoid" id="A0A1E5R2A1"/>
    <dbReference type="OrthoDB" id="5783963at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025160">
        <property type="entry name" value="AATF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039223">
        <property type="entry name" value="AATF/Bfr2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012617">
        <property type="entry name" value="AATF_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15565">
        <property type="entry name" value="AATF PROTEIN APOPTOSIS ANTAGONIZING TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15565:SF0">
        <property type="entry name" value="PROTEIN AATF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13339">
        <property type="entry name" value="AATF-Che1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08164">
        <property type="entry name" value="TRAUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AATF leucine zipper-containing" evidence="5">
        <location>
            <begin position="197"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Apoptosis-antagonizing transcription factor C-terminal" evidence="4">
        <location>
            <begin position="441"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="386"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="131"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="143"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ81014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="575" mass="64811" checksum="26B658266E879F1B" modified="2017-01-18" version="1">MAKKTLSEQIAELANKPKIQDFDIEDEDFQVGTNNDEKNNYSDDNSDEGDDEETKKQHYVKMGTSKLRSDLNDDVVTGVLRDTKYTGLKGSRDALFGNGADKQMSSGEEEEEEEEGEEEDEEDEEDEENSSESAVDLDQVSSSEEEQSENDDQGEDDQQSDSDEDSDVEDKKSKLLKINAQARQTLSKSYTASQTKDALKGYSIIQQNKFFDNIIDSRIKLQKAINVANTLPITGKSWDLLNNSKTEKLINENMDLLSTVLNKIVDIRLLLQKNDKINTSDSAQSKEIGHKRSFENVRESTQTLDSQLNKYRKVVLYKWSNKINQTQGVGSSGAATNGISSKKFSAINQTSDVQVETQLADQQRLLKRTFLNRRGVVPMNFEEDYKSGSLSKLKPTDSQQNSDMVNDDEXNIDIPKNYNPRLKSNIDTSENPYIFDDEDFYRVLLNDLVDKKINNANNTNSSSSGAGQQQVRLITSSTNKVKNKNIDTKASKGRKLNFQIQEPIAHYEAPVGNQTKWSDEQIDEFFAGLLGQGINFNEEDEEVDNNESDEDVENIDGSNAEELENIKNDDIKIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2B0</accession>
    <name>A0A1E5R2B0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dynamin GTPase</fullName>
            <ecNumber evidence="2">3.6.5.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g4042</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.5" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2B0">
        <property type="interactions" value="1351"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2B0"/>
    <dbReference type="InParanoid" id="A0A1E5R2B0"/>
    <dbReference type="OrthoDB" id="5061070at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1240:FF:000002">
        <property type="entry name" value="Dynamin-1-like protein isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019762">
        <property type="entry name" value="Dynamin_GTPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003130">
        <property type="entry name" value="GED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF235">
        <property type="entry name" value="DYNAMIN-RELATED PROTEIN DNM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02212">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00302">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00410">
        <property type="entry name" value="G_DYNAMIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="14">
        <location>
            <begin position="25"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="13">
        <location>
            <begin position="723"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="572"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="582"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="601"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ81032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="809" mass="90423" checksum="E0DA91DB030F9919" modified="2017-01-18" version="1">MSSLEDLIPVVNKLQDVMYDAGIDTLDLPVLAVVGSQSSGKSSILETLVGKDFLPRGTGIVTRRPLVLQLNNIAEDSPLISDYQQNEDLSSDPSTVAENENSHEVTLEEHLKKNNSNYRTDVSSEWGEFLHIPGKRFYDFRDIRKEIENETSRIAGKNKGISRIPINLKVYSPRVLNLTLVDLPGISKVPIGEQPPDIEKQIKNLILEYIAKPNCIVLAVSPANVDLVNSESLKLAREVDPVGKRTIGVVTKLDLMDNGTNALDILSGKVYPLKLGFVGVVNRSQHDIQENKSVDEALRSEEEFFHRHAVYRAISNRCGTRYLAKLLNKILMNHIRDKLXDIKARLNTLIGQTEQELEQYGGTAVITKENRAGLILQLMNKFATNFIASIEGTSSNISTKELCGGARIYYIYNKIFGRSLQSINPVSNLSIMDIRTAIRNSTGPRPSLFVPELAFDLLVKPQISLLLEPSERCVELVYEELMKICHNCGTNDLSRYPKLQTKLVEVVSDLLRERLGPTRTYVESLIDIHRAYINTNHPNFLGASNAMAEIAQDRKKKSAAIAEGKRVAEQAAARKFAEEQNESGSESSSNSAGSSKAAGKKSVENPDSKFDRVGGRHDPVLNHSEKLFGDASKQQKDSFLNYFFGKDAHRENIALNGENGFNSKXASQNMALNGSKPRSDDGAFAFSNLNLESNGNTFVNSINYADELDAEEGHLTEKEELECELISRLIVSYFSIVREMIEDQIPKAIMCLLVNHSKEVVQNRLVTKLYKDEYFDELLLEDENLAQERDRCVKLLETYREAFSIISDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2E6</accession>
    <name>A0A1E5R2E6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA (guanine(10)-N(2))-methyltransferase</fullName>
            <ecNumber evidence="9">2.1.1.214</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g4134</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM11 methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.214" evidence="9"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2E6"/>
    <dbReference type="FunCoup" id="A0A1E5R2E6">
        <property type="interactions" value="832"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2E6"/>
    <dbReference type="InParanoid" id="A0A1E5R2E6"/>
    <dbReference type="OrthoDB" id="296065at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043527">
        <property type="term" value="C:tRNA methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160102">
        <property type="term" value="F:tRNA (guanine(10)-N2)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RlmKL-like_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016691">
        <property type="entry name" value="TRMT11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059073">
        <property type="entry name" value="TRMT11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370">
        <property type="entry name" value="RNA METHYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370:SF3">
        <property type="entry name" value="TRNA (GUANINE(10)-N2)-METHYLTRANSFERASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25904">
        <property type="entry name" value="Tmrp11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017259">
        <property type="entry name" value="tRNA_mtfrase_TRM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51627">
        <property type="entry name" value="SAM_MT_TRM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10 14" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 10 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 10" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3 10" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA (guanine(10)-N(2))-methyltransferase TRMT11 N-terminal" evidence="13">
        <location>
            <begin position="3"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA large subunit methyltransferase K/L-like methyltransferase" evidence="12">
        <location>
            <begin position="203"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="264"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="270"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF25904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ81071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="482" mass="55138" checksum="CB918E3B6B787A01" modified="2017-01-18" version="1">MPKYLIFLVQQHFSFRRPELESLCDLFNIKISNLDDIDKNLENPFFIVNDIPNDTVARKLLSRSILCKGIYELYECAEDGSYDTLHKQLSNNKTLHLQIQNNNENKDNSSVNTDFKFDFEFFGGNRKYSAKQKIEIINTFQHLDIKTNINLKTPGTTYTLIEHYLPLTGQTSSPTPHKVYFTRLVSLSRRTGQNVLDKYDLAKRPFRGTTSFEAELSLVTCNIAQINHGKLLYDPFVGTGSFLCAAAEFGALCFGSDIDGRMIRGHTHRTPSNAKPNSSERSLNGKTKKSNDTSKYQTNGIKTNFLYYGYPDQLLDVVTMDFTHNSLRNSLKLDTIICDPPYGIREGIKVLGAKDPAKHERIQTVLRNGQPAYLHRDYIPTKKPYSLDLLLQDILEFASQRLDLGGRLCFWMPVANSNDIETVVPLHSNLELKYSCVQEFNKWSRRLLCYVNRGESYNGPTNDISDGSARNEFRKDYFNGFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4R6</accession>
    <name>A0A1E5R4R6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA (guanine(9)-N1)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.221</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">tRNA methyltransferase 10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">tRNA(m1G9)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g3796</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>guanosine(9) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(9) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43156"/>
            <dbReference type="Rhea" id="RHEA-COMP:10367"/>
            <dbReference type="Rhea" id="RHEA-COMP:10368"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.221"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.221" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4R6"/>
    <dbReference type="FunCoup" id="A0A1E5R4R6">
        <property type="interactions" value="787"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4R6"/>
    <dbReference type="InParanoid" id="A0A1E5R4R6"/>
    <dbReference type="OrthoDB" id="278300at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052905">
        <property type="term" value="F:tRNA (guanosine(9)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18089">
        <property type="entry name" value="SPOUT_Trm10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028564">
        <property type="entry name" value="MT_TRM10-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038459">
        <property type="entry name" value="MT_TRM10-typ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016653">
        <property type="entry name" value="TRM10/TRM10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007356">
        <property type="entry name" value="tRNA_m1G_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563">
        <property type="entry name" value="TRNA (GUANINE-9-) METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563:SF13">
        <property type="entry name" value="TRNA METHYLTRANSFERASE 10 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016323">
        <property type="entry name" value="tRNA_m1G_mtfrase_met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51675">
        <property type="entry name" value="SAM_MT_TRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase TRM10-type" evidence="12">
        <location>
            <begin position="116"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="311"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="314"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR016323-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR016323-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ81858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="377" mass="44114" checksum="BE5D06FD69A7BAF3" modified="2017-01-18" version="1">MSQTSEKDQVETSEKGQVNASSTTSRPLDDNEKKELRELYINKNKQLLTSFGPKPEGMSKSQWKKVCKRKQWESEKEVYREQLRQKRMEKRHARKSKIQEYLQRGEPLPDHLKRKTRRPTVEQQDSKMKIIMDCAFDELMNDKEIISMSNQITRSYSANRNFAYYANIEIASFNKRLKQRFEGKLANTRFMEWKNVKFVENDTVKELSAEDKINTVYLSADATETLDTLESGKTYIVGGIVDKGRYKKLCLNKAQELQIPTKRLPIDEFIKINGRQVLTTTHVIELMLRYFESKDWKTAFEQVLPARKLGHEEGAEEGAEEEAEEEAEEEAEEEEEEEKEEEEEKAEKEAEKEAEKEAEKEAQKEEEVAQNSNAKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4T6</accession>
    <name>A0A1E5R4T6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">U2 snRNP component IST3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3686</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4T6">
        <property type="interactions" value="726"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4T6"/>
    <dbReference type="InParanoid" id="A0A1E5R4T6"/>
    <dbReference type="OrthoDB" id="3971608at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051847">
        <property type="entry name" value="RNA_proc/Spliceosome_comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45880">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN, X-LINKED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45880:SF1">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN, X-LINKED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="34"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="160"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ81878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="191" mass="21764" checksum="0552806A361586D0" modified="2017-01-18" version="1">MNQIRSIRSLSEQELELNILDAQDSWHEEYKDNAYIYFGQLXKSLTEADILTIFSQFGNPVDLRLFYDKQTGLSNGFGYLKYEDQRSTILAVDNLNGVHLLGHTIKVDHAFYRPTKDAQLQKYSSSMEAELAKDXLFTENTKTEPKATLRVTQLLNVQEQPADEDDVNDPMANLLGSDSLLPYDGXTEEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4Y3</accession>
    <name>A0A1E5R4Y3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein kinase MCK1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3757</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4Y3">
        <property type="interactions" value="324"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4Y3"/>
    <dbReference type="InParanoid" id="A0A1E5R4Y3"/>
    <dbReference type="OrthoDB" id="272141at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14137">
        <property type="entry name" value="STKc_GSK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050591">
        <property type="entry name" value="GSK-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039192">
        <property type="entry name" value="STKc_GSK3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057:SF4">
        <property type="entry name" value="PROTEIN KINASE MCK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="48"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="80"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ81950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="445" mass="50973" checksum="7755091293C8E2F3" modified="2017-01-18" version="1">MSGQQQQQQQQQQQQQQQNNNNNNLGYKEFVCNEVTNNRTNETTKMIIKEFRKIGHGAFGTVVQAYIAKVNLGTGELAKTNNITDSGNSNNSLDNENGSTPLQWRGPFAIKKVPAQTEYKSRELDILRVTDHPNIVKLEYYFNYPLPTVVESSAGQHASNNMQNFNQQNTHNVPRTVTKSILFQHLAMECLPETLQLEIQRYTSNKLQLPLKHIKLYTYQLARGMLYLHSFGICHRDIKPSNILVDPNLGILKICDFGSAKKLEINQPSISYICSRFYRAPELILGNTCYTTQIDIWGLGCCFGEMLIGRAIFQGEQPLLQLKEIAKLLGPPDKKFIFFSNPSYDGPLFSKPLFKGSSLERFQKYFGHAGVEGIDLMXKILKYEPEKRISPRRIMAHPFFDELRQELYFFPRGHSQXKKLPELFNFSQYELEVIGNMYSAIVPAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R576</accession>
    <name>A0A1E5R576_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Prefoldin subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3675</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R576"/>
    <dbReference type="FunCoup" id="A0A1E5R576">
        <property type="interactions" value="754"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R576"/>
    <dbReference type="InParanoid" id="A0A1E5R576"/>
    <dbReference type="OrthoDB" id="29646at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027235">
        <property type="entry name" value="PFD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13303">
        <property type="entry name" value="PREFOLDIN SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="5"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ82065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="118" mass="13751" checksum="2B2B509DE7D7FDFD" modified="2017-01-18" version="1">MAQPSIEEQQELQALQAQYEQFKQEYQAVQMKIMELNHQREEYDVVLKTLEQVKDPKRKCYRAVGGSLLETDVEGCMPILKKKSSALTTVIAKYQQELKQAIKSLDEWKAQNAKFQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5B4</accession>
    <name>A0A1E5R5B4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADPH-dependent aldehyde reductase ARI1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3604</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R5B4"/>
    <dbReference type="STRING" id="56408.A0A1E5R5B4"/>
    <dbReference type="InParanoid" id="A0A1E5R5B4"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000191">
        <property type="entry name" value="Methylglyoxal reductase (NADPH-dependent)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="6"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ82096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="339" mass="36946" checksum="60263339313F5578" modified="2017-01-18" version="1">MSKPTVFISGANGFIAQHIIKQLLDSKTYKVVGSVRSAAEAEKLESDFGNHPDLSLVIVEDLSKPNAFDSVFKEHGASFDYILHTASPVNFAAKDLEKDVIVPAINGTKSIFQATVKYAPNVKKFVQTSSVAAMVNVNSSNTTKEVITESSWNPITFEQGLENQLNAYLYSKTAGEKGLWELHKSSNAKFPLVVINPTYVFGPQAFDSSAKGSLNASNEVISAIVQSKLNDDNVTNVKGGFIDVRDVARAQVEAISNSNFNGRRMILDNRNFSSQEVADIINANIPQLKGKIAKDVTLKEVGPGFSANIDSHVSKELLGFEFIDFPKTVIDTVDQILRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5G0</accession>
    <name>A0A1E5R5G0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3721</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R5G0"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="362" mass="38824" checksum="953C5F871C7CB048" modified="2017-01-18" version="1">MDISEHQSSNNHSHPGNNENHHNNSHSNADNKKKISYWVKRIMQPQNTPVSTTXTTTATSTNVVSHXHHRHSTGSTRHTTRKPEPRSSAVGGGGEHRPNTXSSPVKGGSKEKKVREDTADNSGGTTDGKQXDSNRSNKQDIDTTDTXLEVESDRISQNSNQNTIDNSDNFSTTGVISIKTRKSDYSSYTPAPFQKPMAPGLLSQQQTQGQNAYPLQDNGSIKSFTNATITSNSDTINSSIIGIPPASIIDRNKITGNSTTTMSIASQSMINYQPVTNGAASVFSGKVTNHGMTTATTINSSSLNPATPGLSSSSQAGNTAARSRTNTIAEPVMEATTEREEASQRATRASRMTSDVVHNVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R699</accession>
    <name>A0A1E5R699_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">dipeptidyl-peptidase III</fullName>
            <ecNumber evidence="5">3.4.14.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Dipeptidyl aminopeptidase III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Dipeptidyl peptidase III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g3706</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal dipeptide from a peptide comprising four or more residues, with broad specificity. Also acts on dipeptidyl 2-naphthylamides.</text>
            <dbReference type="EC" id="3.4.14.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M49 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.14.4" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R699">
        <property type="interactions" value="642"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R699"/>
    <dbReference type="InParanoid" id="A0A1E5R699"/>
    <dbReference type="OrthoDB" id="4694525at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.30:FF:000001">
        <property type="entry name" value="Dipeptidyl peptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.30">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039461">
        <property type="entry name" value="Peptidase_M49"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23422:SF11">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23422">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE III-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03571">
        <property type="entry name" value="Peptidase_M49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="735"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="739"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="758"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="772"/>
            <end position="786"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ82063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="827" mass="94692" checksum="47725D40DFFEEBC9" modified="2017-01-18" version="1">MPQKTEYDPFLHYADHEPPIDILELKQDLVDQLSMKEEQYAHYMSKASQAGTRIVLRQVSHESEGLFDMIMDNHKHVMAHCGGQYPKGSHEHERRYYLEYCSQFLNNLGNYTFFGDSKFIPQCSREYWYELLRMRGVHTSDKIQVNSVSPIVSLVDKLTYKDLIETVFFINEANVKLGFPNENNHSSYYFGNVSNDDMHFLTEKVYTKYGILPDNTRVWKEEGTVLKEETLLEENDQKKKCTIKIIHFRILIASVFLKNKTELTGYPQGKFTVEKSEAGDILGDETRITAEFIFGDHSNELARIVSYLRKAIPHVSNALQLAMLQDYIKYFETGDGEYHKDSQRKWVKDSCPMVECNCGFIESYRDPSGVLGEFQSIVALQNKKRTKSFQDLVQHASEFIATLPWXKXFENDSFKAPDFTALEVLTFTGSGIPVGINIPNYSDIKEEIGCKNIAMSNVLDLDSKIKTKPSFILENQIQEFKKYSGEAFNIQVGLHELIGHGTGKLLSXIETSNNGKESGKEFGKENSKSYNFDIDNPPLGLDGKPVSTHYSLNETWFNKFGPISGAYEECRAECIGMYLLPNKKILECFGISDKIEQEKVIFNGYLLMARSGLNALQYWSPETNQIMQPHMAARFIVFKQFLKDKNFCKIVLNENETDFFIELNANLIESVGVKLVGDLLQHLHVYRCSGDVERGTEYYNKAAQVPEELLKLRPIVLSKKLPRKQIIQTNTFLGAPMEKNGKTPMEKNGKTPMEKNGKTLSNNSEKTFIDNTKNKETPKDNIKDSIKTTSNKISKPKNKITKGKKIYIKHYPETALGMLDSFAEREY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7C8</accession>
    <name>A0A1E5R7C8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Mitochondrial carrier protein RIM2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g3301</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>5-methyl-UTP(out) + UTP(in) = 5-methyl-UTP(in) + UTP(out)</text>
            <dbReference type="Rhea" id="RHEA:73523"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:63527"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7C8">
        <property type="interactions" value="458"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7C8"/>
    <dbReference type="InParanoid" id="A0A1E5R7C8"/>
    <dbReference type="OrthoDB" id="269120at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015218">
        <property type="term" value="F:pyrimidine nucleotide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990519">
        <property type="term" value="P:pyrimidine nucleotide import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000079">
        <property type="entry name" value="Mitochondrial carrier protein RIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000088">
        <property type="entry name" value="Mitochondrial carrier protein RIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049562">
        <property type="entry name" value="SLC25A33/36-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45829">
        <property type="entry name" value="MITOCHONDRIAL CARRIER PROTEIN RIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45829:SF4">
        <property type="entry name" value="MITOCHONDRIAL CARRIER PROTEIN RIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="80"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="194"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="299"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="17"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="56"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00093195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ82819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="392" mass="43778" checksum="C4C6D1BD32CF90AF" modified="2017-01-18" version="1">MPXNNNIAQLEKDVVESAPYLGSDESNVNMKNVDINSDMLQTNQHTRDSKLTQASQQQQQQQQPQQQQKASEVASKPDGVKPWVHFVAGGVGGMAGAVVTCPLDVVKTRLQSDVYKNMYKTAVKQGDSAGKNMILHFRETIGILGSLYKVEGFNSLFKGLGPNLVGVIPARSINFFTYGTTKEFYSKTFNNGKEDPYIHLMAAATAGWATSTATNPIWLIKTRLQLDQKGKRQYKNSLDCFKSILKNEGIFGMYRGLSASYLGSIEGILQWLLYEQIRRVVKERSTKNDKDKTGWETFKDWAARSGGAGCAKFFASIATYPHEVVRTRLRQAPKEHGKPKYTGLLQTFRVIIKEEGLASMYSGLTPHLLRTVPNSIIMFGTWEFMIKLLSNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7I5</accession>
    <name>A0A1E5R7I5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">dynamin GTPase</fullName>
            <ecNumber evidence="1">3.6.5.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3375</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.5" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7I5">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7I5"/>
    <dbReference type="InParanoid" id="A0A1E5R7I5"/>
    <dbReference type="OrthoDB" id="5061070at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008053">
        <property type="term" value="P:mitochondrial fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019762">
        <property type="entry name" value="Dynamin_GTPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056495">
        <property type="entry name" value="LIS_MGM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF212">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24550">
        <property type="entry name" value="LIS_MGM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00410">
        <property type="entry name" value="G_DYNAMIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="10">
        <location>
            <begin position="245"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="9">
        <location>
            <begin position="843"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="181"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="461"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="740"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="181"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="194"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="11">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ82841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="943" mass="106065" checksum="C4A90F0442608FDF" modified="2017-01-18" version="1">MNNIYKTVGIRRHSTLLFKRSSFATTARTNIFXSSRVSKSXPGNAVYSQNSHGSFMLPVSKNDLFHFTNLHRSISGASFIPKVIGKVVKLPVYLGGGLAAFGSYVAYKVEETSQYTQDKLGGLKDFVDGIGGWFSKEEGEDSTGNNKKRNNNNNNSGATEGLAGASLLASLDDADADGIRRNXQGEEERGKANDDEDDEEKEEAEEEEEDEVSSKDTTGDDMVNLTKQMIEIRNILNRLGSSNANLTLPSIVVIGSQSSGKSSVLESIVGKEFLPKGSNMVTRRPIELTLVNTLDDEITADFPQVRKYNIKNFNEVKTLLTDMNLQVSSSECISEDPIQITIKSNKVPDLSMVDLPGYIQIESVDQPIELKNKIKTVCNKYLQAPNIILAISPADVDLANSSALKASRAIDPKGERTIGVITKLDLVPPEYANQLLNNKKYPLKMGYVGVINKLNKKNTIGSQSKSRNFKNSIFGTSPDNQAGKSATSQTGTNASALSPVNEQFEITYFKENXKYFQNCEVTSKKLRHKLIKILEVSMSNALEPTSTMIEQELDEASYRFKVEFNDRQLTPKSYLLTQIDVLKLAIKELQANFTRNELKSILKADLDQKVLDILSTRYWKESNNTMVYEDKRHSKNTGGSGSNDDDDVYWHKKLELASSSLTKIGVGRLSTQLVTNSILTELNNIIRYINPNYEKGFINELVNDTAINVLNSKFFATADQVENCIKPFKYEIDIEDRDWKDSREHSIKLLKEELKQCNERLLTIKNGVGNGKLQQVVSYLNKVKEQSQNRSQFESTGSFQPQETLGFSKLMIERGLEYIDLNNRSQLLNFRVKFMRSSKCGSTKDMDRCPEIYLNAINDKLTSTAVLFLNVELLSDFFYNFPIDLDRKISTMSPDQMELFAKEDPKIARHIELQNRKELLELALSKIDSILVFRKTYKGYMHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7I6</accession>
    <name>A0A1E5R7I6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosducin-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3148</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7I6"/>
    <dbReference type="FunCoup" id="A0A1E5R7I6">
        <property type="interactions" value="740"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7I6"/>
    <dbReference type="InParanoid" id="A0A1E5R7I6"/>
    <dbReference type="OrthoDB" id="10257948at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21148">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ82856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="211" mass="24223" checksum="1945F8BF46F044B2" modified="2017-01-18" version="1">MEKKIIKAYQTEKIQEQNGIVSCSHGDSKLDIQNSKKETFAEDSEEDIDAMIEELENEDSDDIFTQQYRESKMQAFYDSLKKIEQNVTSGKYGKVNEIHNENELMHAVLNNKTGKTCIMFFQPNFLKCMELEQHMQNVASQLYNTVFYKINVADCPFLVSKFKLKVLPFCVCYSSQLDGSKILSTLVGFPQFDGAEDFTTEEALIKCLLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7L8</accession>
    <name>A0A1E5R7L8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">5-aminolevulinate synthase</fullName>
            <ecNumber evidence="12">2.3.1.37</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">5-aminolevulinic acid synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Delta-ALA synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Delta-aminolevulinate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g3210</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the synthesis of 5-aminolevulinate (ALA) from succinyl-CoA and glycine, the first and rate-limiting step in heme biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>succinyl-CoA + glycine + H(+) = 5-aminolevulinate + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:12921"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="2.3.1.37"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 12">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 12">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 11">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.37" evidence="12"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7L8"/>
    <dbReference type="FunCoup" id="A0A1E5R7L8">
        <property type="interactions" value="484"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7L8"/>
    <dbReference type="InParanoid" id="A0A1E5R7L8"/>
    <dbReference type="OrthoDB" id="10263824at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00375"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003870">
        <property type="term" value="F:5-aminolevulinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06454">
        <property type="entry name" value="KBL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000006">
        <property type="entry name" value="5-aminolevulinate synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010961">
        <property type="entry name" value="4pyrrol_synth_NH2levulA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01821">
        <property type="entry name" value="5aminolev_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF102">
        <property type="entry name" value="2-AMINO-3-KETOBUTYRATE COENZYME A LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8 12" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="14">
        <location>
            <begin position="137"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="54"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU910713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ82871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="571" mass="62706" checksum="39B1A120CB86E439" modified="2017-01-18" version="1">MEAIAKQSMKLCPFIKNSLVKNPGKYISQSGELTAQLYNRCPVMHQAVRNYASGTASSSSSSSAGAAAASPAASTDSSTASSTAADLATQESSYNYAQHMDTELNNKRRENSYRQFNNINRLAKEFPMAHRDDPSDKVTVWCSNDYLSLSKNERVIDKMRETLGRYGAGAGGTRNIAGHNQHGMYLESELAKLHKKSGALVFSSCFVANDAVISLLGAKFKDLVIFSDELNHASMIVGIKHANVPKHIFKHNDMQDLEKKLQLYPKNQPKLIVFESVYSMCGSVAPIDKICDLAEKYGCLTFLDEVHAVGLYGPHGAGVAEHFDFDTHLTEGSNSIAGSISPFTGNHISKSVMDRVDMITGTLGKSFGTVGGYVAGDALLIDYIRSFAPGFIFTTSLPPSVMRGAQEAIKISKESLDLRVTQQKVTQYVKDNLERIGVPCMGNPSHIVPVFIGNAELAKRASDLLLNKHKIYVQAINFPTVARGTERLRITPTPGHSYELCDKLIDAVDDVFNELGLLRIQDYENIDLNGHNKNGITSRLWTEDQLSLSNKDLHKNVFVDDLEFLDSSRGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7M6</accession>
    <name>A0A1E5R7M6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3224</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7M6">
        <property type="interactions" value="951"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7M6"/>
    <dbReference type="InParanoid" id="A0A1E5R7M6"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="228"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="442"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ82912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="618" mass="70398" checksum="B463931C661D05B9" modified="2017-01-18" version="1">MANIKQNHKNKHQRPVDINDILGLTTQTKPKLLSKQERINFLQQKQRAGQLPHGLAKAHXNXLKASGQFSASGGQKVEQNVVPVPAKTNLEPFAITYSEHEDTSLRNAEDELIPKIMMINPFKLKEMKVTKKNKAKKSLELLPARKRHVVSSVSAASNENWEQKSRGAMTNEDWKKFNQKFGISSYECSVLPIKSWQDETDMLPLKILDVIIGKLGFEEPSTIQKCSIPNILSGSDFLGVAKTGSGKTLAYVIPLVVRSMLQDTVRRQVSSCYSLILSPTRELAQQIEQVGNQFGVCDFISIVGGKSLEEYSDKIVHQRRKNLEAKSLVIVATPGRLIDCLNMGIVQLDQLDSIVYDEADKMVDLGFEDQLNQIHAKIPNHGNLQKIMFTATLENKKLENLVKARLSXPNCVKLQSTDDVPRINQFFEFLPVSKQRTQTQKKFTELCNFLKKNVYQYKKIPIIIFVNYKKTADELLQRLIDQNYKAEAIHGSKSQDQRERTLQKIKTHQTEILIGTDVAARGIDIPNVAIVINYELGTNFEEYVHRIGRTGRGGKNGESLTFLCEDEFPHTKFFNSFHKFLTKRYPKEHLHFPKKLLKQHQIDDYDLKDVSQNDFIVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7N3</accession>
    <name>A0A1E5R7N3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Vitamin B6 transporter TPN1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Transport of pyridoxine protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3222</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Thiamine-regulated, high affinity import carrier of pyridoxine, pyridoxal and pyridoxamine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7N3">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7N3"/>
    <dbReference type="InParanoid" id="A0A1E5R7N3"/>
    <dbReference type="OrthoDB" id="5428495at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4160.10:FF:000007">
        <property type="entry name" value="Pyridoxine transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026030">
        <property type="entry name" value="Pur-cyt_permease_Fcy2/21/22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31806">
        <property type="entry name" value="PURINE-CYTOSINE PERMEASE FCY2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31806:SF17">
        <property type="entry name" value="VITAMIN B6 TRANSPORTER TPN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002744">
        <property type="entry name" value="Pur-cyt_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="274"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="313"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="359"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="429"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="522"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="557"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00083325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ82906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="583" mass="64839" checksum="D96FDCB05B8626CE" modified="2017-01-18" version="1">MPSSSGIKSTSDDNHITTINLHLDNEVERKVDLTSXHEQLTPLSTSEKATFANNTNAFQKFTDFLKSVSQRVEKLGVVETTGIQRVPPYARGTKKQQLIQVTGFWISASGGLSSMSSFFLGPLLFGLSFKQSLLSGLVSCFVGCIVAAYCAMMGPQSGCRQMVTARYLFGWWLVKFVALVAIIGVLGWSVTNCVVGGEMLASISNEKIPLWVGVIIVSIVSIFVAIFGIKQVLKVEMYFSVPVMTVFTLLYICASDKYHLINDYQPPAGTSMKGAWLSFFSICYSVTSTWGSITADYYILFPEDTPKSQIFMLTLWGIMIPTTYVGVLALLLASSAMSYEPWLDEYNDFGMGGLLAAGFSRWGGFGKFCIVILLLSLISNNIVNTYSAAFGIQLSSVRMAKIPRWFWAIVVTAIYLVAALVGRNHFSTILSNFLPMIGYWISIYFAILLEENVIFRKHFLYLYTKEFPNLISTQSTSSPSSSTLSSVASMQNNNRQDKSFKFKKIRYNWDAWNDYSVLTRGYAAFVAFLFGAAAVAVSMSQTYWIGPIAKKLDEGDIAMWLSMGVSGLVYPLLRYWELKKFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7P7</accession>
    <name>A0A1E5R7P7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nuclear segregation protein BFR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3355</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7P7"/>
    <dbReference type="FunCoup" id="A0A1E5R7P7">
        <property type="interactions" value="225"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7P7"/>
    <dbReference type="InParanoid" id="A0A1E5R7P7"/>
    <dbReference type="OrthoDB" id="2195113at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042175">
        <property type="term" value="C:nuclear outer membrane-endoplasmic reticulum membrane network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039604">
        <property type="entry name" value="Bfr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31027:SF2">
        <property type="entry name" value="LEBERCILIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31027">
        <property type="entry name" value="NUCLEAR SEGREGATION PROTEIN BFR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="320"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="361"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="90"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="164"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="244"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="408"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="326"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="372"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="474" mass="53537" checksum="3A8622EBDC411C3E" modified="2017-01-18" version="1">MSQNNTSNNTSGNYKRIPKPDVSVRDKKLELLNNQLKKLNLEIDSVRGKIDKSMNSSDNTKRTELTDKLKEIIKVQSDLKQKRNGVFDSLKNLDANLKRKQAEIDSKLNGVSLNKKFQSIDDINNRLEQIEQDMSTGDLSIVEEKILVKEMASLNKLKKNFVLVQPIKVEIDQLKIEISNLKNQLNTELNSKAVSQEFEKTNNELNELRSSQSTEQNAKNLLFNKRAALYAKRDEIYSKIKTIREDFENEYASFKLKLEQEKVKRNEENKLNKLLEQKETDLSKLNEKLIHAKNPAFQQELSSIENVLLVLDPSYEKPKPKSLFPTNTTTTTTTSTTTTTNTPGKLTPIVSKKFESTIVVGGGKKGKKNKHAGASSTSSSNTAGGKFSLEPSLIGILSELDVDIPITQDQVAKTVEQLKAKHKEYEEHQDEQTEINIKAVQDKIEQVKKAYDTKEEEIKQQVASEAAAKVEAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7U6</accession>
    <name>A0A1E5R7U6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcription initiation factor IIA large subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3349</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIA subunit 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7U6"/>
    <dbReference type="FunCoup" id="A0A1E5R7U6">
        <property type="interactions" value="495"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7U6"/>
    <dbReference type="InParanoid" id="A0A1E5R7U6"/>
    <dbReference type="OrthoDB" id="3973283at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07976">
        <property type="entry name" value="TFIIA_alpha_beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.100:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.18.10">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004855">
        <property type="entry name" value="TFIIA_asu/bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009088">
        <property type="entry name" value="TFIIA_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12694">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12694:SF8">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03153">
        <property type="entry name" value="TFIIA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01371">
        <property type="entry name" value="TFIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47396">
        <property type="entry name" value="Transcription factor IIA (TFIIA), alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50784">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="112"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="166"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00074154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ82958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="240" mass="26913" checksum="90614C37639F9242" modified="2017-01-18" version="1">MSNKEVSTVYESIIENVINEVRVDFENNGVDEDTLQSLKQIWQNKLSESKTAVYSWDTEADVESMNTTNGAQRELEHGVDANGTSDAAVGPDGEIVVKGELKEGQEVEIEINNGGENDNDNSNSNIGNNDVIPKSEDNQVLVAKKNAEELEMLEKLKQEQKDAKKTALLETDEVNSDLDDSDDDYLNSDDEDQEEGNILLCLYDKVGKVRNKWKWSLRDGIATVNNKDYTFKRANGESEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R819</accession>
    <name>A0A1E5R819_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Translation initiation factor eIF2B subunit epsilon</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">eIF2B GDP-GTP exchange factor subunit epsilon</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3178</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-2B gamma/epsilon subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R819"/>
    <dbReference type="FunCoup" id="A0A1E5R819">
        <property type="interactions" value="1210"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R819"/>
    <dbReference type="InParanoid" id="A0A1E5R819"/>
    <dbReference type="OrthoDB" id="424572at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04197">
        <property type="entry name" value="eIF-2B_epsilon_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11558">
        <property type="entry name" value="W2_eIF2B_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000066">
        <property type="entry name" value="Translation initiation factor eIF-2B subunit epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035543">
        <property type="entry name" value="eIF-2B_epsilon_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051956">
        <property type="entry name" value="eIF2B_epsilon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056764">
        <property type="entry name" value="LbH_EIF2B3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003307">
        <property type="entry name" value="W2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044123">
        <property type="entry name" value="W2_eIF2B_epsilon"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45887">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45887:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25084">
        <property type="entry name" value="LbH_EIF2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02020">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00515">
        <property type="entry name" value="eIF5C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51363">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="W2" evidence="9">
        <location>
            <begin position="539"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="434"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="509"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="442"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="517"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="534"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ83055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="719" mass="82861" checksum="903ED4F4F724B067" modified="2017-01-18" version="1">MAKKNSSGSNKKNSFVNSATGVNGEAERLQAVVLTDSFQTRFMPMSKVKPRCLLPLANKPLISYTFEFLTQANVEEVFLVCGSHIEQIQDYIYTLKWLPFKIHIINSVESQSVGDCMRDIDNRGIISGDFILISGDLVTNLDFNKAWDFHQKKKAQDKDYIVTMCLSECSDQFDKLRGQDLATFIYNEESQKCIYYQEIQSARSRKKASIDIDPDLIEEDNEDEDFVLRNDLIDCHVDICTEAVPAIFQENFDYQYLRKDFVKGILTSDLLKKHIYAYIAENEYAARVESWKTYDTISQDLINRSIYPLTIENNNSYSYEMPHIYKDENIVLAQSCKIEKNTVIGKNTKLGEETFIENSILGENCIVGNNVVIKNSYIWDNCHIESGSVVNHSIIADGCKINGKLKDCVINYNVHLNDNYSGVRLTNLKFHNKKNHDSFASDSEEDADDDEEEEEDEEEEEDDDDEEEGDNEDQNAPKLYESSTEDDSDEEDDIINSLQYQMDELYTSDTSISSTTKQHKKKKKRTMSSTSYMTEEESSEEEEDFIKEGIATIERAMENNHDLDTALLELNTLRMSMDVTYHEVRVSVITAMIKRVYTFINTQTLAPKEAVIKIFTSWGGLLSRVTFDEDEYFDLLDIIEQEIDELGTFEDPATITFLAFNTLYDIDVLEEDVVLKWWQTNVSTELKQAPINRMVAKWIEWLQTTDDDEEESDEEDESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R878</accession>
    <name>A0A1E5R878_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative 26S protease subunit YTA6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3289</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R878"/>
    <dbReference type="STRING" id="56408.A0A1E5R878"/>
    <dbReference type="InParanoid" id="A0A1E5R878"/>
    <dbReference type="OrthoDB" id="10251136at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19509">
        <property type="entry name" value="RecA-like_VPS4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000022">
        <property type="entry name" value="Fidgetin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000093">
        <property type="entry name" value="Fidgetin-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF17">
        <property type="entry name" value="FIDGETIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="448"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="182"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="263"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="270"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="285"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="320"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="364"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ83071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="685" mass="77215" checksum="D21AFB1EEAADD9B2" modified="2017-01-18" version="1">MGLDKYAIPSSFTLLQSLQLLLFISQSKYQNYKESYNLTIAQTQIQDHQANETFLQNNYKNIQNVAQYLKNGIKIIQNEFQVKDVYENYVNLDVVSDLRILRFEINEETERLKNLLGKKNDFGSFLKHRIFNSHDNAEREQVESLKNKKLVNQRQIEMKHQEQETQKQALLENQIRKEVKQEIIQEKKRTSRSSMERKAEKRISTSRPNLNVETEQHRSAKISTNPNGSLVSPNRATKSSSDVSTGLNAAILAFNGARASPTLQARRQFSPPPPPPPPPQMATPTSNNSRGNNSSNNTASGAKTPVSTHHPRKSFERHTSTGTTSHSNMNSTHVTYNGIPQAGQSKPTRRESPVVLTSDGSVQNDRRKSKFIEDKNKKQKSENDLDPRLQNLPLEPLVITQIMNEVLVSDAKIHWDDICGLEQTKAVLKETVVYPLLRPDIFKGLREPVTGILLFGPPGTGKTMIGKCVASECNATFFSISSSTLLSKFLGESEKLIKTLFLVAKRLAPSIIFIDEIDSLLSSRSGDGNENESSRRIKTELLIQWSNITNDQVLVLGATNLPWSIDEAARRRFSKKLYIPLPDYQTRLMQLRKLLHNSSLTTEQFDILAQITEGYSSSDLTTLCKEAAMCPVRELGDQLLYVDSKKVRLITAADVENSLQVVKPSVSKQNLVNFETWSQLYGSQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R8E6</accession>
    <name>A0A1E5R8E6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3317</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R8E6">
        <property type="interactions" value="1134"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R8E6"/>
    <dbReference type="InParanoid" id="A0A1E5R8E6"/>
    <dbReference type="OrthoDB" id="10260596at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.2650:FF:000001">
        <property type="entry name" value="40S ribosomal protein S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001377">
        <property type="entry name" value="Ribosomal_eS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014401">
        <property type="entry name" value="Ribosomal_eS6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018282">
        <property type="entry name" value="Ribosomal_eS6_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11502">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01092">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002129">
        <property type="entry name" value="Ribosom_S6_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01405">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00578">
        <property type="entry name" value="RIBOSOMAL_S6E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ82803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="265" mass="30230" checksum="03945A1897F9B3F5" modified="2017-01-18" version="1">MVAKSNGLDVYSNKLVRFXEDTGNAITYLQQLNIAYPVNGSQKIVEIDDEHRVRCFYDKRIGQEVDGEYVGDEFKGYVFKISGGNDKQGFPMKQGVLIPNRIKLLMGKNVSCYRPRRSGERKRKSVRGAIVGNDLAVLSLVIVKRGEQELEGVTDTVVPKRLGPKRANHIRKFFGLSKEDDVRDFVIRREVTKGDKTYTKAPKIQRLVTPQRLQRKRAQKALKIKNAQAQREAAAEYSQLLAKRLSERKQEKAEIRKRRASSLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA06</accession>
    <name>A0A1E5RA06_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">5'-3' exoribonuclease</fullName>
            <ecNumber evidence="14">3.1.13.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AWRI3579_g2533</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Possesses 5'-&gt;3' exoribonuclease activity. May promote termination of transcription by RNA polymerase II.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Possesses 5'-&gt;3' exoribonuclease activity. Required for the processing of nuclear mRNA and rRNA precursors. May promote the termination of transcription by RNA polymerase II. Essential for vegetative cell growth and chromosome segregation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with RAI1; the interaction is direct, stabilizes RAT1 protein structure and may stimulate its exoribonuclease activity. The interaction also stimulates RAI1 pyrophosphohydrolase activity, probably by recruiting it to mRNA substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the 5'-3' exonuclease family. XRN2/RAT1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.-" evidence="14"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA06">
        <property type="interactions" value="1117"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA06"/>
    <dbReference type="InParanoid" id="A0A1E5RA06"/>
    <dbReference type="OrthoDB" id="28245at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18673">
        <property type="entry name" value="PIN_XRN1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1050:FF:000002">
        <property type="entry name" value="5'-3' exoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12390:FF:000005">
        <property type="entry name" value="5'-3' exoribonuclease 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12390">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027073">
        <property type="entry name" value="5_3_exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041412">
        <property type="entry name" value="Xrn1_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004859">
        <property type="entry name" value="Xrn1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017151">
        <property type="entry name" value="Xrn2/3/4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341:SF41">
        <property type="entry name" value="5'-3' EXORIBONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341">
        <property type="entry name" value="5'-&gt;3' EXORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17846">
        <property type="entry name" value="XRN_M"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03159">
        <property type="entry name" value="XRN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037239">
        <property type="entry name" value="Exonuclease_Xrn2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 14" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 14" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 14" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="Xrn1 N-terminal" evidence="17">
        <location>
            <begin position="1"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Xrn1 helical" evidence="18">
        <location>
            <begin position="311"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="Xrn1 helical" evidence="18">
        <location>
            <begin position="631"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="101"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="451"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="390"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="122"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="467"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="484"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR037239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF03159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF17846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OEJ83728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="899" mass="104094" checksum="6514D51557B9524F" modified="2017-01-18" version="1">MGVPSFFRWLSRKYPKIISPVIEEENDLYTNTNPNGELDNLYLDMNGIVHPCSHPENKPPPENEDEMLLAVFEYTNRVLNMARPRKVLYIAVDGVAPRAKMNQQRARRFRSAKEAAEKDGGPMEDDENLDDDDNSTSESGKSKKKTWDSNAITPGTPFMDKLASGLRYWISFKLSTDPGWKDLQVIISDATVPGEGEHKIMSFIRAQRQDPQYNPNTTHCIYGLDADLIFLGLATHEPHFKILREDVFEQTIDKRSNRNNSRYDPKRELSEQEKQDLEAKDKKKPFLWLHISVLREYLRIELFVPRLSFPFDFERAIDDWVFMCFFCGNDFLPHLPSLDVRENSIDSLVRIWKEILPTFKEFMTLDGHLNYECVEKLLRQLGSNESRVFMTKRSKEMRKLENQHKRKLQQLENEFDTSTAVEIGESGGNXTNAYNKKFKFDQNGQTLAVDKKNDEEEEDSYDPSSSAQSTSTLEPTDSNMNKAEQMRSKLKQLKDKRTFKQTQVQDKFQEYLSENNLPSAPTPSSGIYDSDQIVKLYEPGYQERYYKIKFGCRNAEEIHKTKRNVVKSYFEGISWVLLYYYQGCQSWNWYYPFHYAPFAQDFVNLGELLDEQDDMTDDGVTDMDTKVKTTKREIAFELGEPFTPFEQLMSVLPASSGHTLPKIFRELMKSPDSEIIEFYPTEFPIDMNGKKMQWQGITILPFINEKKLLKAVRAQYVNLSDADKERNKLKKEILLMSKENVNYGKLKAQVYEHQAEVEIHHFNSGLSGIVKPNDEDSFDINGKSPCPVSGGFPDLENKNFMKFNFANVQHFENKSIILNGYIPHFPALTQVDRDNLIHGFGTSYNRRGGYGNQXYGQGHGMYFRVDDMKANIIKTGPYGNTQYKPRLGGYKSFYFSRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAG5</accession>
    <name>A0A1E5RAG5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">Glycylpeptide N-tetradecanoyltransferase</fullName>
            <ecNumber evidence="3 7">2.3.1.97</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2961</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Adds a myristoyl group to the N-terminal glycine residue of certain cellular proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N-terminal glycyl-[protein] + tetradecanoyl-CoA = N-tetradecanoylglycyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15521"/>
            <dbReference type="Rhea" id="RHEA-COMP:12666"/>
            <dbReference type="Rhea" id="RHEA-COMP:12667"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:64723"/>
            <dbReference type="ChEBI" id="CHEBI:133050"/>
            <dbReference type="EC" id="2.3.1.97"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the NMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.97" evidence="3 7"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAG5">
        <property type="interactions" value="951"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAG5"/>
    <dbReference type="InParanoid" id="A0A1E5RAG5"/>
    <dbReference type="OrthoDB" id="60315at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004379">
        <property type="term" value="F:glycylpeptide N-tetradecanoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000042">
        <property type="entry name" value="Glycylpeptide N-tetradecanoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000903">
        <property type="entry name" value="NMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022677">
        <property type="entry name" value="NMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022678">
        <property type="entry name" value="NMT_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022676">
        <property type="entry name" value="NMT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11377:SF5">
        <property type="entry name" value="GLYCYLPEPTIDE N-TETRADECANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11377">
        <property type="entry name" value="N-MYRISTOYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01233">
        <property type="entry name" value="NMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02799">
        <property type="entry name" value="NMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015892">
        <property type="entry name" value="N-myristl_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00975">
        <property type="entry name" value="NMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00976">
        <property type="entry name" value="NMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycylpeptide N-tetradecanoyltransferase N-terminal" evidence="10">
        <location>
            <begin position="72"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Glycylpeptide N-tetradecanoyltransferase C-terminal" evidence="11">
        <location>
            <begin position="249"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ83899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="462" mass="53321" checksum="B7F75181493C6050" modified="2017-01-18" version="1">MSKQNINKGETHGGAPDLDATQLAKIQEMLKALRMQHLENSNEHQPKNLEDYKFWKTQPVPKFDESIEKEGPIDSLKTVADISRVPVPLKAIPGFEWCDLAVDDHASRDLDDVHELLNENYVEDNTSMFRFNYTKEFLRWALTPPGYYKTWFVGIRVSSSNKLVAFISAVPVTLNANGKTVKAVDINFLCVHKKLRSKRLAPVLIKEITRRVNLENIWQALYTAGVVLPSPVTRCRYTHRPLNWAKLHDIGFTRLPEGATETQMIAKYTLPKNPHLKMEPLVEEQDIDEXFKLLNNYQKKFKLYQDFEKHEFVHMMKTRENVLYSFFVRDQASGKITDFISFYLLPFTILENKIYKDLNVAYLFYYATDEKHXETQDLLKTRLCELVNDACIIAGKQLKVDVFNALTSQDNDLFLKDLKFGAGDGVLNFYLFNYKMKPIDGGMAAYENNLKDXTSELGVVML</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAK4</accession>
    <name>A0A1E5RAK4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2800</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAK4"/>
    <dbReference type="InParanoid" id="A0A1E5RAK4"/>
    <dbReference type="OrthoDB" id="3972078at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184671" evidence="2">
        <location>
            <begin position="22"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="297"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ83939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="327" mass="36938" checksum="FB982156807540AB" modified="2017-01-18" version="1">MKVILWGVFFTVLLSAEKTCAHIVLNKADLWFEDFKRHFNESLLDIMVESNHLAHVLNSLESDSQNPRIASTEFSFLKKKEELDSSDENDQVENQKVLNILQDALCMISDKSSINFKTHNNQEQEKVNNLGMEPPTMRKAFLNSMQTIENGIFTKFENSQLNMFVSQFFWEATSKIVNIKKQIQGSKLVKNKTKEEAYKKKMSTYLAHVITAAKEISSFKPEKISTEEINSFDLISYSKEDEKFLGNLIGMIICKNIKPLMDDGNVSAASQEAVNVLNDIATYLGFVTITIGLCTTFIAPLPLLCWASVASVGVASLALMLRIIKNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAR5</accession>
    <name>A0A1E5RAR5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein ISD11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2759</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I LYR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAR5">
        <property type="interactions" value="463"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAR5"/>
    <dbReference type="InParanoid" id="A0A1E5RAR5"/>
    <dbReference type="OrthoDB" id="275715at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990221">
        <property type="term" value="C:L-cysteine desulfurase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20264">
        <property type="entry name" value="Complex1_LYR_LYRM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045297">
        <property type="entry name" value="Complex1_LYR_LYRM4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051522">
        <property type="entry name" value="ISC_assembly_LYR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056326">
        <property type="entry name" value="ISD11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13166:SF7">
        <property type="entry name" value="LYR MOTIF-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13166">
        <property type="entry name" value="PROTEIN C6ORF149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23511">
        <property type="entry name" value="Microp_apicomplexa_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="100" mass="11752" checksum="9B01204D70CAC2FA" modified="2017-01-18" version="1">MSTPQITGKQFIXLYRDYLRTAGSFTNYNFKNYFLRKAKRDFRNYRQQAVSGSTKESGLSQLDTFNKLRTELAVLKRQSVVSQLYTFDKLVVEPINPHHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAX9</accession>
    <name>A0A1E5RAX9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Vacuolar protein sorting-associated protein 17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAX9">
        <property type="interactions" value="85"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAX9"/>
    <dbReference type="InParanoid" id="A0A1E5RAX9"/>
    <dbReference type="OrthoDB" id="9976382at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030905">
        <property type="term" value="C:retromer, tubulation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053055">
        <property type="entry name" value="VPS17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47433">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47433:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="611"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="651"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="174"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="651"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="769" mass="87323" checksum="670F086B7305B363" modified="2017-01-18" version="1">MHSSDNDFDDDDYDYDNNPFSSPFVSSXGGXIPGIPEASNPFAEEAHAAPEDTSATLASNYEXDQPESSNERVEEDTPSDNTPNXSQDTSADQVNEHTTKLQHLRETHEKFLXKYQLLVKVSKITRTLPANSSDKNYNXRVGNGATGPTTTGSISSNSTNNGRNTVGKKDGYRSSSNDSSSFTSRPGDLVIYMEVFTNLPTFRKKTNKNVIKSYKEFENFCVYLQDFRLDHFHEFFPPALPLNKTSYGLSNAEDLIQCKINFQKWFDLRICGNVKIMKLEELAIFLESDYNTYLPMNGCMNVNNNTTTSVISTNHSLDHLNSNDTMIAPTEQLKRKTLKQFQPPFDPVQELATFRPMIKYWRKNLQMLQVGLFKMCKQRQALSYNESVMGKNYIKMSENMVQIDGFDKNSAINNDRSFSNNTISRSRLNSIGNNSNINSIGGNEVAKKFSNMYKRLGKTMVTIGDMDSIMTTFNMATLYDGLEWCCRDLYNVKEQLTNRHLLMRELNNVSANLKKNQEHIRKLRSKRDTNPLKIDECMKNLQKVCEQEAFWKTRLAKITENLQLESETGIIKIWEQQNIQFMKDYALKNIEYERKKLNILERIRSDVRKTDKSGGLSRLGRPSFDQSMSGMNTGADAPQVDTTTIANPNVVNTDSNGHSKNIFKQPNPSMSSNDSWSGDNRKEHKYYSEIEKELLANGNKQLLSSANNESLLIQNDLQEDQESGALGESAAVNKEFHGNNEQNDNDTAASFVDWNTKKYAANLLGRSTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB00</accession>
    <name>A0A1E5RB00_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">arginyltransferase</fullName>
            <ecNumber evidence="2">2.3.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2608</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the R-transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.8" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB00">
        <property type="interactions" value="642"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB00"/>
    <dbReference type="InParanoid" id="A0A1E5RB00"/>
    <dbReference type="OrthoDB" id="74183at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004057">
        <property type="term" value="F:arginyl-tRNA--protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030700">
        <property type="entry name" value="N-end_Aminoacyl_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007472">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007471">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21367">
        <property type="entry name" value="ARGININE-TRNA-PROTEIN TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21367:SF1">
        <property type="entry name" value="ARGINYL-TRNA--PROTEIN TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04377">
        <property type="entry name" value="ATE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04376">
        <property type="entry name" value="ATE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-end aminoacyl transferase N-terminal" evidence="6">
        <location>
            <begin position="21"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="N-end rule aminoacyl transferase C-terminal" evidence="7">
        <location>
            <begin position="190"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="500"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="502"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="519"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ84076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="620" mass="71832" checksum="EDC3CEF210DA6DB5" modified="2017-01-18" version="1">MEIADRLIISKPIYIREPYTQCGYCKGQKPHESDYFGLQSWYDVHSKNGGLSSTKKVTDEVEIDNCTLGIHVELVSVQQYDEFCNLGFRRSGKYXYKPDLLRNCCRLFTIRTTLPQCKVSKELKQCVKRFKKFTNAENNIPLELQNKNTGGKNKQPQKGFDLLKELYLAEQYSENNDFYTKFEPSVFSQEKYELFVKYQQQVHKDYLNNEQSFKNFLCHAPFAEDVMMGSEEEWEELNNWKEIYAENYLRKSNTNKTEAMTENGEIKSTDTRQKSKNLFQNRKTRYGPVHECYYHKGKLIALAVLDFLPTGVSSVYFIWDPDYSKWNLGKISALRELALVTILQRDYYYLGYYIDDCPKMMYKKKFGGDLLDVCNNQYVPINKVAEYIKHGKFFVVANDTETKSPSSADEEDNSYYDENDHSELPLNDAVKFDVNKPLINIAEKIYGEKEGFAIQHSNEAVAKLMEYGIEYPSMMFNDYYKLKPMIPSFSSLDNHTIVGSNSSTHSSHTSTSTSTLSLEVEEEEEEEEEEDDGDIPGYTKEIYNIPAVIPGLLPMWQIAQIIQSGAINSINNKLMLYNTRTNQVRKMEDFSKENKRVKKCICNVIRLIGLKATAKSLIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB09</accession>
    <name>A0A1E5RB09_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">General amino-acid permease GAP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3089</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB09"/>
    <dbReference type="InParanoid" id="A0A1E5RB09"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000017">
        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004762">
        <property type="entry name" value="Amino_acid_permease_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00913">
        <property type="entry name" value="2A0310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF1">
        <property type="entry name" value="GENERAL AMINO-ACID PERMEASE GAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="83"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="367"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="415"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="444"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="487"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="527"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="10">
        <location>
            <begin position="83"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ84085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="599" mass="65533" checksum="831F1521664C1D8C" modified="2017-01-18" version="1">MSDIEKTKQNYDVNVEQNTSLYEHTSTSSFVDGPDPNKSGWENFKDSFKRVHVEDLDPNLTAAERIAIKTARSPLKRXLKTRHIQMIAIGGAIGTGLFVGSGSSLRAAGPAGLLIGYFISATFILCVISALGELAVEFPVSGGFTTYATRFVDESFGFAANWNYLMGGLAVLPLEIVSASITVNFWGTPKKYRDGFVALFWVVIVTINMFGVKGYGEAEFLFSAIKVTCVLGFIILGIVLVCGGGPNGHYMGGTMWHHPYGAFVGDNGVQRFKSTMSVFVSAAFAYSGVEMVGLAAAETANPRKSLPRAAKQVFWRIALFYIVALSLVGLLVSHKDPRLIGTSSADAAASPFVIAIKSHGISGLPSVVNVVICIAVLSVGNADIFATSRILVALTEMGHIPRWCNFDYIDRQGRPLIALIVTSLFGCIAFVAESNKEGEVFDWLMALSGLSALFTWFGISLCHIRFRRALTAQGRSTDELCFKSPTGICGSYYSCFIIFLVICATIWTSSAPSGFKEMTSNELASNWFEXLLSLPIFLVWYFGHKIYSKSFKIFIPASKIDIDTGRRETDREVLLMEIAEEKAALAQKNFLVRAYFFWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB25</accession>
    <name>A0A1E5RB25_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Actin-related protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3060</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RB25"/>
    <dbReference type="FunCoup" id="A0A1E5RB25">
        <property type="interactions" value="310"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB25"/>
    <dbReference type="InParanoid" id="A0A1E5RB25"/>
    <dbReference type="OrthoDB" id="5132116at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="142"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="335"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="398"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="151"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="335"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="546" mass="61977" checksum="2A6B81E4474F59A3" modified="2017-01-18" version="1">MTVALADTQYRCCVVIQLGTTSTVVGYSNMEQPKFIIPTNYYHYAGKSSSDGINTKDINADTYRYGFYEILDELDENENKGDIFSIINSDYSKVDFDRLTGFLQYIYNDKLGLSSNELPLVVTLPYSAMYVNDFTRFGSGNCSNPTDETSNQNKNNHGNYNNQRNHHNGHKILSKYDKTPKYTLFYNKLKNLLLDDMQIPVINILNEPLSTSLSLGRVNSLVVDCGGNRCNVSCIIDGHVVSSISSKIAGNYLDYMIMEKINDKLKQMNITNSINSNNSVQQWKNSWLWLKDFKSLLLNCDPQLSVLNGSGVNGYNEYTSTGKAITNEMINTEHEYEQEHEQEHEQEHEQEHEQEQEQENTVNFLLKEQGGITLQFKESELKQLSDCLFDPVFSTTNTNTSTTTNQNNDSNNNQNNNQNNDNNNEQYFGVGKLIMTCIKQCVTETLAELSNTANTNDSNNTGSNTRSNELQEYTILESLLNNVLITGGSTLLSGFENRILYEISTHYPKFKISTFANPVLQDRLLSNYHAGVAMSNLNWELNDWKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB76</accession>
    <name>A0A1E5RB76_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">IMP-specific 5'-nucleotidase 1</fullName>
            <ecNumber evidence="12">3.1.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g2634</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">IMP-specific 5'-nucleotidase involved in IMP (inositol monophosphate) degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>IMP + H2O = inosine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27718"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="3.1.3.99"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 12">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ISN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.-" evidence="12"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB76">
        <property type="interactions" value="101"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB76"/>
    <dbReference type="InParanoid" id="A0A1E5RB76"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006190">
        <property type="term" value="P:inosine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071590">
        <property type="term" value="P:nicotinamide riboside biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071592">
        <property type="term" value="P:nicotinic acid riboside biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009453">
        <property type="entry name" value="ISN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28213">
        <property type="entry name" value="IMP-SPECIFIC 5'-NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28213:SF1">
        <property type="entry name" value="IMP-SPECIFIC 5'-NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06437">
        <property type="entry name" value="ISN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028836">
        <property type="entry name" value="ISN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR028836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ84131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="441" mass="50895" checksum="153971CA01BFF634" modified="2017-01-18" version="1">MSSKYRIDYHLKRHRKDEFIDWIKGMLSIPFCLLHSYNLEDVMNATNELETDLKGXKITEEAFQRYLAIFQDIESLVQEKIKDNNDGKSRLDQLVPSIGPIFTPLPLARAFAIQSDKRRISARRFVSVSFNDVRHILNTAQVLHFTDPNVSKLKLVTFDGDVTLYEDGGSLTNDNKIILPMLEILSHGVKVGLVTAAGYDTPEMYETRLSGIVEALYMREDIPYNYKQNLVIMGGESNYLFQYYEDKHLEKRGFLKVEPHQSWMLPEMVXWDDNDIKAVLDLAEXILHKLKKKLNLSPETQIIRKKRACGIMPGHKVDEKTAENIKPLKFTRENLEEMVLTLQKIMENYPPAQRIQFSCFDGGSDCWCDIGGKDLGVRALQYFWDKENPIKPTETLHIGDQFAPMVGCNDFKSRLSGCTLWIASPEETNELLMDILQRWQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBE4</accession>
    <name>A0A1E5RBE4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Metal homeostatis protein BSD2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2504</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBE4"/>
    <dbReference type="InParanoid" id="A0A1E5RBE4"/>
    <dbReference type="OrthoDB" id="10003116at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031398">
        <property type="term" value="P:positive regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019325">
        <property type="entry name" value="NEDD4/Bsd2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13396:SF5">
        <property type="entry name" value="NEDD4 FAMILY INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13396">
        <property type="entry name" value="NEDD4 FAMILY INTERACTING PROTEIN 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10176">
        <property type="entry name" value="NEDD4_Bsd2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="506"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="21"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="133"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="252"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="565"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="23"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="136"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="257"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="567"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="7">
        <location>
            <position position="437"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="7">
        <location>
            <position position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ84220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="666" mass="74165" checksum="ED78023980F0E037" modified="2017-01-18" version="1">MENNGNHPAYNNNSILTHANQINTNNNTNNTNNNTNNNTNNIHNYSSAGSSDVRGSHNEFSGNDSVGVPMLEEYRMNTLSVGSGSMQNGDDQRPESPVPLIASHPELRESFRRMCGYFNILDRVFHPKRQYSLMANNNNNNTNNNNNNNNNGNNGNNSSSANISNADRDVDDNTTTTTTTTTINDNNPYTNNDHSNSLEEPIGQGNDGVFSNLTAKPESLQERRERTRRLEMLAGMNEGYEDYLFRYYNNSEANDSNNTTNNNTNNNNNTNNNTNNNTNTNTNTNTNTNNRNTNARQRPNYEDMYAGEFERQDKPPTYEEASSDTVPPYWDLSPESAXYYDEICIDGLPAGNLINFLWNCAISSSFKFFGFLITYILHTSHAAKEGSCCGLGITFLLFAYSIIPNNVSHKVGKGKDIQKIHLSNPDQHTGDFLNTKLPEFTTKTEDIAKATMTAALNSGDEKNLSKSSSSSSSFLSDNNNAYDTLDTFHSTLSHGYEETNNERNKYMNILAIVLFIIGTGMIFHSIYSYCRIKRIEKSIMRQEESIQELQQQQQAEAHTEQLRQLESRAQQRANSSSHGLLNNNTQNTDASDTVTVSDINELENAVMNDSGAVSIGAQSNRANSAASLSTLTLPFNETRVPFDDLSNIDHADIQSDTSDSPSLRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBP5</accession>
    <name>A0A1E5RBP5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">26S proteasome regulatory subunit 6A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g2703</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBP5"/>
    <dbReference type="FunCoup" id="A0A1E5RBP5">
        <property type="interactions" value="997"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBP5"/>
    <dbReference type="InParanoid" id="A0A1E5RBP5"/>
    <dbReference type="OrthoDB" id="9443236at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000009">
        <property type="entry name" value="26S protease regulatory subunit 6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000076">
        <property type="entry name" value="26S protease regulatory subunit 6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000037">
        <property type="entry name" value="26S protease regulatory subunit 6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="212"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ84306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="432" mass="47727" checksum="D4851A67B1C740D9" modified="2017-01-18" version="1">MSTLEELANQVLPGDEELDTDILNSSAQELQTRTKLLENEIRIFRSELQRLQYEQAQMVAKIKDNKSKIKNNKQLPYLVSNVVEIMDLNDDIEGDEKTNQGGNVNLDNTASGKAAVVKTSNRQTVFLPMVGLVDPSTLKPNDLVGVNKDSYLILDTLPSEFDSRVKAMEVDEKPTETYSDVGGLDKQIEELVEAIVLPMKQAEKFKDMGIKAPKGALMYGPPGTGKTLLAKACAAQTNATFLKLAAPQLVQMFIGEGAKLVRDAFALAKEKAPTIIFIDELDAIGTKRFDSEKSGDREVQRTMLELLNQLDGFGSDDRVKVLAATNRVDVLDPALLRSGRLDRKLEFPLPSEEARAQILQIHSRKMTIDDSINWQELARSTDEFNGAQLKAVAVEAGMIALRNGQSKINHEDFVEAIGEVQARKTKSASFYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBQ7</accession>
    <name>A0A1E5RBQ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Copper-transporting ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2525</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBQ7">
        <property type="interactions" value="455"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBQ7"/>
    <dbReference type="InParanoid" id="A0A1E5RBQ7"/>
    <dbReference type="OrthoDB" id="432719at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043682">
        <property type="term" value="F:P-type divalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055070">
        <property type="term" value="P:copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd02094">
        <property type="entry name" value="P-type_ATPase_Cu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000002">
        <property type="entry name" value="Copper-transporting ATPase 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000043">
        <property type="entry name" value="Copper-transporting ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520">
        <property type="entry name" value="ATP7, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520:SF8">
        <property type="entry name" value="P-TYPE CU(+) TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="341"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="381"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="539"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="574"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="943"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="972"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="11">
        <location>
            <begin position="127"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ84334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1062" mass="117232" checksum="68B4AF3594F2DE9B" modified="2017-01-18" version="1">MDTETSNSPDNMVFQQVHRVVFGIGGMTCSACVNTIQKQVDNYFTQECVAKGIVLKLCNVTLITEEGVVEYTTDVNVESASISPLEISNQLKEVVEDCGFDCNIMSDQIISKEDLSKSSSSSSTSVKTVHLHVYGMTCAACTNSIEDALSQLAGVVSCSVQLVTQECEVTYDHNKQTIRDIVATIEDCGFDCSLQEDTHQDQQLQILQRVKEIKYWANSAIKASICSVTIMFLYMFIPMIIMKTRHQKMDSMSSNMPMNDGTPIADNTGNMSDQQXKHAYGHARNGVIGTFPYVQVGIPGLYLSDIIGLSIASYVQFYLGKNFYKSSFAALKHYTGTMDTLVCLSTSIVYIFSVVSMLRNMIISDRLENENNSTNKIMPNVLFETSCMLISFISIGKYLENKAKARTSTALSKFMTMTPTNCLLYDIETKTSXEIDVKYLETNDLVEIKPGMRIPCDGVIVEGESELDESIITGESHLIYKTANDHVIQGSINGHGHFIFKALQVGESTKLSQIIKVMKLAQLSKAPIQKYADYIASRFVPAVLVTSTLTFVSWVLICKFITVPQIFQQNENGYLYMCFQIAMSVIVVACPCALGLAAPTSVIVGTGVAANNGVLLKGGEILQNVSELDCFVFDKTGTLTTGEMSVETFKKNEEALNEEFALNTLWELCYLVENLSEHPIAKSIVRYTSTKIAGHKLATMSQHHQIASQEIIMGQGMKAVVHCLGANEKDYTEYSVLIGNMKLFQDSPALQKSIEQSMNIDNNANAKSQAFVAINNKFVGTFLVEDSYKKDGPQIINYLLSKKYDVYMCTGDNKNAALKTAADLGIPFQNVYYEVSPVEKANIIKGLQTEHXKKVGFVGDGINDSVALVQADIGISVSNGTDMAMEASDIVLLQNLTTDTVRNDDMDVGIAPTTRTSSDNSLKGLIYAIDISIKTFNKIKSNFFWSVLYNALMLPIAMGFLVPFHISLHPMFAGAAMICSSISVVLNSLLLKRWRKPEIENIRMPAKSAATSSFFEQGKHSLAKLLTFFNKSSNRDRAREEDEGLVVNELNPSTTEIEMEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBV6</accession>
    <name>A0A1E5RBV6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein transport protein Sec61 subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g2972</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Necessary for protein translocation in the endoplasmic reticulum.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the SEC61-beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBV6">
        <property type="interactions" value="144"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBV6"/>
    <dbReference type="InParanoid" id="A0A1E5RBV6"/>
    <dbReference type="OrthoDB" id="5401193at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005784">
        <property type="term" value="C:Sec61 translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030671">
        <property type="entry name" value="Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016482">
        <property type="entry name" value="SecG/Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13509">
        <property type="entry name" value="SEC61 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03911">
        <property type="entry name" value="Sec61_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006398">
        <property type="entry name" value="Sec61_beta_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR006398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ84377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="90" mass="9434" checksum="D721CB940C6DAF99" modified="2017-01-18" version="1">MSGSXAPPGGHKTLQKRRQAQAAKEKKASQTPASATQAGLAGSTNNILKIFTDEADGWKVDPSAVLFLAVAFIFSVIALHVISKVTGKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBW9</accession>
    <name>A0A1E5RBW9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Elongation factor 1-beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2594</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the EF-1-beta/EF-1-delta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBW9"/>
    <dbReference type="FunCoup" id="A0A1E5RBW9">
        <property type="interactions" value="775"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBW9"/>
    <dbReference type="InParanoid" id="A0A1E5RBW9"/>
    <dbReference type="OrthoDB" id="331763at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005853">
        <property type="term" value="C:eukaryotic translation elongation factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00292">
        <property type="entry name" value="EF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.60:FF:000001">
        <property type="entry name" value="Elongation factor 1-beta 1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036219">
        <property type="entry name" value="eEF-1beta-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018940">
        <property type="entry name" value="EF-1_beta_acid_region_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049720">
        <property type="entry name" value="EF1B_bsu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014038">
        <property type="entry name" value="EF1B_bsu/dsu_GNE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribsomal_bS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001326">
        <property type="entry name" value="Transl_elong_EF1B_B/D_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11595">
        <property type="entry name" value="EF-HAND AND COILED-COIL DOMAIN-CONTAINING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11595:SF21">
        <property type="entry name" value="ELONGATION FACTOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10587">
        <property type="entry name" value="EF-1_beta_acid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00736">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01182">
        <property type="entry name" value="EF-1_beta_acid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00888">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54984">
        <property type="entry name" value="eEF-1beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00824">
        <property type="entry name" value="EF1BD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00825">
        <property type="entry name" value="EF1BD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 4 9" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Elongation factor 1 beta central acidic region eukaryote" evidence="8">
        <location>
            <begin position="68"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Translation elongation factor EF1B beta/delta subunit guanine nucleotide exchange" evidence="7">
        <location>
            <begin position="105"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="159"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ84381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="191" mass="20928" checksum="4DB7AF8641DE2162" modified="2017-01-18" version="1">MHIYTSTAATQADVTVYKAFQTAFPEFSRWFNHIASKADEFETLPAGKAPAASASAAAEEEDDDDVDLFGSDDEEVDEAAEKLKAQRLAEYHAKKAAKPAKPAAKSIVTLDVKPWDDETDLEAMVAHVKGIEIDGLVWGAHTWVPIGFGIKKLQINCVVEDSKVSLEELQEQIEEAEDYVQSSDVAAMQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBY0</accession>
    <name>A0A1E5RBY0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Proteasome subunit alpha type-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2614</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBY0"/>
    <dbReference type="FunCoup" id="A0A1E5RBY0">
        <property type="interactions" value="1087"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBY0"/>
    <dbReference type="InParanoid" id="A0A1E5RBY0"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03754">
        <property type="entry name" value="proteasome_alpha_type_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000026">
        <property type="entry name" value="Proteasome subunit alpha type-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034642">
        <property type="entry name" value="Proteasome_subunit_alpha6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 7 9" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="8">
        <location>
            <begin position="4"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ84396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="269" mass="29814" checksum="B7810ECC2AFFD563" modified="2017-01-18" version="1">MSGYDRHITIFSPEGKLYQVEYAFKATNQTNLNSIAVRSPFCSVVINQKKVTDKLMDSKTISFIFKISPTIGMVATGPIPDAKNAATRAISEAAEFRYKYGYDMPCDVLSKRMANLSQVYTQRAYMRPLGVILTFVSYDEEKGPLIYKSDPAGFYAGYKATATGPKQQELTTSLEKYFKKLDNDDGATATANAATDDTATNTALDNENYVEGTSWEKTVELGITQMIDVLGTDFTFKDLEIGVATKENGFFTLTSEQIEERLVAIAEQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDG9</accession>
    <name>A0A1E5RDG9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cytosolic Fe-S cluster assembly factor NBP35</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">NBP35</name>
        <name evidence="10" type="ORF">AWRI3579_g1937</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NBP35-CFD1 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Required for biogenesis and export of both ribosomal subunits, which may reflect a role in assembly of the Fe/S clusters in RLI1, a protein which performs rRNA processing and ribosome export.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDG9"/>
    <dbReference type="FunCoup" id="A0A1E5RDG9">
        <property type="interactions" value="595"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDG9"/>
    <dbReference type="InParanoid" id="A0A1E5RDG9"/>
    <dbReference type="OrthoDB" id="1741334at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904564">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly scaffold complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000427">
        <property type="entry name" value="Cytosolic Fe-S cluster assembly factor NUBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03038">
        <property type="entry name" value="NUBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028601">
        <property type="entry name" value="NUBP1/Nbp35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264:SF35">
        <property type="entry name" value="CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR NUBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264">
        <property type="entry name" value="NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="75"/>
            <end position="82"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>ligand shared with heterodimeric partner</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>ligand shared with heterodimeric partner</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ84948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="325" mass="35264" checksum="AC5046E521A72410" modified="2017-01-18" version="1">MSSKLEKNPTDYNLVEPEPEHCPGPESENAGKTDSCTGCANQQICESLPKGPDPDIPLITDNLSQIKHKVLVLSGKGGVGKSTFTTLLAWCLSADEEVEVGCMDLDICGPSMPRMLGCHQELVHQSNSGITPVYVADNLATMSIQYMLPDDDSAVIWRGSKKNALIKTFLKDVDWGDHIDYLIIDTPPGTSDEHITINKYLKDSVIDGALIVTTPQEVALLDVRKEIDFCRKAGINILGLVENMSGFVCPGCHNESKIFRATTGGGKSLCKELNIPFLGSVPLDPRIGKACDNGISFVDEYPDSPATDAILDIVEQLRDTLEDSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDH7</accession>
    <name>A0A1E5RDH7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative transporter SEO1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2017</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDH7"/>
    <dbReference type="FunCoup" id="A0A1E5RDH7">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDH7"/>
    <dbReference type="InParanoid" id="A0A1E5RDH7"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000065">
        <property type="entry name" value="Putative MFS pantothenate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF15">
        <property type="entry name" value="TRANSPORTER SEO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="364"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="400"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="459"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="523"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ84960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="575" mass="65127" checksum="EBFE52B062505050" modified="2017-01-18" version="1">MVKVLYGELKWGFIPVKRIVEDYPENSVKAVETDESSEYSNAEKKDLDQQTSSATSAAVAEKSGVESANVAEDANVIEYEYRDEADRKWWKFFDEQEYTIPAHIKNKKKQWNWFEPNTNGAEKKLLVKLDILLAFYSCMAYWVKYLDTVNLNNAFVSGMQESLGMTKSDLTTVQNMFSIGNTIFQIPFLFVLFKCPLNYTLPVLDIVWSLFTVGACKSQTLGQLKACRFFVGAAEAPAYFAYQYLFGSFYQSTELVRRSTFYYFGQYIGVLSSGALQGCLYKYGTATFEGWRVAFLVDAMISLGVGFLGFYMLPGNDPYNIYSIFLSDDEIRLLRKRTRRNVFKASDNLLDWKVWLKLFSQPKIYVLSLFNAFLWCCSNASSGSYLLWLQSLKEYSVVKLNQLSMTSAGIGLVLLAVFGLYADLFNSKYQAIILSQCVNFTGNIILSCYSKASKGAKYFAFNIAYSAWAAAPTGYMYMAIMSRDAEERSKILVVANIFAQTMSIISNKFAFNAQDAPTYKHGFPFAAAMAVCLSLCSLVVLYMYKRQERTLAKSNGIVLYNSKTGENYPLDKEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDW6</accession>
    <name>A0A1E5RDW6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">APC/C activator protein CDC20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2036</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat CDC20/Fizzy family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDW6">
        <property type="interactions" value="198"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDW6"/>
    <dbReference type="InParanoid" id="A0A1E5RDW6"/>
    <dbReference type="OrthoDB" id="10263272at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF8">
        <property type="entry name" value="FI02843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="9">
        <location>
            <begin position="425"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="558"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="45"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="10">
        <location>
            <position position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="767" mass="84701" checksum="48CE3ED8921492F7" modified="2017-01-18" version="1">MSNGLVFSPNRPYKKSKGNDENLGTPSKLLRKASFSGKGADKQPSRSSGAALSNSHVPNILGNTPASKLNSLSGSYCPQKVFKKSSSISMPSKQPCLKRQASMSSLSISKPSLKKANSLAMLNKTLSKIGDNNNGSMKXLHAEERLHNSALTHGSYPCGNTSNSSKTNGIRKFNRPAMRNANPALQKTRSFDISSTFFGKSSFDSKSSVSTNNNTKQVDNATXLFSLNSNGQKDGFGSSGLMTQLPDRFIPASTNDKANSTLASVLASTKLSNKSVAQNITEALXPHTNAQNYXDDSEEDINQTASSIIDSPPPPANASPETHLKSINKKFFQKTVAQACGIDANQRILQYLPKAPLPSLKRSQSYSLENSRSRYTYEYYSGVRIDEFSTNSRSSSPSLPTDFMSDRTKPNYQIRKVNQSPERILDCPEFFDDFYLNLIAWNKRNVLAIALNQAVYLWNGDSGDVLQLLDYSXQPLMKITSVMWSDDNFHISVGFEQGFMEVWDTETMKKIRKMXGLEKSRIGVQSWLCNLIASGNKDGMIKINDVRVKNHVIQSWDDHHHGLEVCGLAYRSDGLQLASGGNDNSMIVWDTRMSQPQFVKRQHQAAVKALSWNPDISTLLASGGGSNDQKIHFWNTNSGIRVNTIDTGTQISSLHWGQSTNNKREIVATGGEPKNAISVYNYENRLKVAEIINAHESKIISSQLSPDETTLCTIGGDENLKFFKVFDSKKPKYTGIRAQDDFIDDDHLDGVENDGKLKSPHKNITIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDX7</accession>
    <name>A0A1E5RDX7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutathione hydrolase</fullName>
            <ecNumber evidence="8">2.3.2.2</ecNumber>
            <ecNumber evidence="8">3.4.19.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Gamma-glutamyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Gamma-glutamyltranspeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1974</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Cleaves the gamma-glutamyl peptide bond of glutathione and glutathione conjugates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 8">
            <text>an N-terminal (5-L-glutamyl)-[peptide] + an alpha-amino acid = 5-L-glutamyl amino acid + an N-terminal L-alpha-aminoacyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:23904"/>
            <dbReference type="Rhea" id="RHEA-COMP:9780"/>
            <dbReference type="Rhea" id="RHEA-COMP:9795"/>
            <dbReference type="ChEBI" id="CHEBI:77644"/>
            <dbReference type="ChEBI" id="CHEBI:78597"/>
            <dbReference type="ChEBI" id="CHEBI:78599"/>
            <dbReference type="ChEBI" id="CHEBI:78608"/>
            <dbReference type="EC" id="2.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>an S-substituted glutathione + H2O = an S-substituted L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:59468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="ChEBI" id="CHEBI:143103"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 8">
            <text>glutathione + H2O = L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:28807"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:61694"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Sulfur metabolism; glutathione metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the gamma-glutamyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.2" evidence="8"/>
    <dbReference type="EC" id="3.4.19.13" evidence="8"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDX7">
        <property type="interactions" value="171"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDX7"/>
    <dbReference type="InParanoid" id="A0A1E5RDX7"/>
    <dbReference type="OrthoDB" id="1081007at2759"/>
    <dbReference type="UniPathway" id="UPA00204"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103068">
        <property type="term" value="F:leukotriene C4 gamma-glutamyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.40:FF:000001">
        <property type="entry name" value="Gamma-glutamyltranspeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000101">
        <property type="entry name" value="GGT_peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00066">
        <property type="entry name" value="g_glut_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686">
        <property type="entry name" value="GAMMA GLUTAMYL TRANSPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686:SF9">
        <property type="entry name" value="RE13973P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="43"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="485"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="503"/>
            <end position="505"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="527"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="556"/>
            <end position="557"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="579"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="678" mass="76127" checksum="05E8B953E5871A30" modified="2017-01-18" version="1">MRLADPPGKTWRNKNSEPKVKQLRTKMEKHTNHTRSNVRVKRLLLAFFTLIICLSLYIFNAKSKKLATGEILKDFHNECPNKLSEKQVESLKAVTKDHNIDINPLYRAPTMHPNSSEVLRTGEHGALSSDLELCNELTYKKIIDKYGMENVNSADLAVPLGLCIGMVNFFNSGIGGGGYAVYTDTTRNNENNEWFDFRETCPLAWDNDVHNLTKVGGMSVGVPGEIMGLYELHHQKGLGKVTWSQLLDPIIELGQTGWSVGPVLGAALSIYEDYFNNNYDSWSFVFNKDKSLKKSGDWIQRPTLSAMFQELANNGSVAPFYDPEHWIVKSMVKSVQKAGGLLTGQDFANYKVMQGKPLSTTITTXNLFQYDVQTVGGSSSGAALIAGLNIMKSFPYRVGGDYMKESVYELVETMKWMASSRSRLGDYFLESTDNDTNNYPQRILDVLSDSWRINATHKMRENYLHTFDDFHEYDPKYEMNDPHGTAHFSIVDSHNGAVSLTTTINLLFGSLVHDPVTGVIFNNEMDDFSDPHGQSNAFDLKPSIYNLPEPGKRPLSSTVPTVIIDELGRPDLVIGASGGSRIMTSVFQTIVRNYWYGMPLLESIAYPRVHHQLLPNQLEVESKSMIGSTTIKQLQEIGYTDIVETXPKSVVNAIKRDRISTLHAVSDYWRKRGISVAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE36</accession>
    <name>A0A1E5RE36_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1938</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RE36"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="354" mass="40673" checksum="812F31E128876CE4" modified="2017-01-18" version="1">MXXGEITNCTFWFASLNEEEKNFYGQCDILQYTHQDIVEHLEEIKNTETFNKMCSNQEDFEQMLNNYEKGNIKQEAGSKDNANKEPEFVIQNLVXGDGENYFFSEWLCSLMIQSTSLISREMAWSSLLHWLSSGSIGSRLATSGATGKAQWMIQLEDESLEKKGNNGFSTLMLIDASTKFKKVFSSCSILSTFNSNHFINIQRLTTKIVRSSFAELYMCKENLKELQEDQKLRESLTADLDAKREERDSKTLSLFLKMLNSKKKEVWRLEKQSAQYKRLLIENNVSFEKDEKGSDTDVSDISLENVSMSEEENETTPEEEDLXDVQINQYANTATDDRGAQRVKYNKADPFLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE83</accession>
    <name>A0A1E5RE83_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="13">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AWRI3579_g2355</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:21249"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="13"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE83">
        <property type="interactions" value="1020"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE83"/>
    <dbReference type="InParanoid" id="A0A1E5RE83"/>
    <dbReference type="OrthoDB" id="10248617at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.270.10:FF:000011">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.150:FF:000004">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000008">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000016">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1110.10:FF:000007">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1800.10:FF:000007">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1100.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009674">
        <property type="entry name" value="Rpa2_dom_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06883">
        <property type="entry name" value="RNA_pol_Rpa2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13 20" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="17">
        <location>
            <begin position="41"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="16">
        <location>
            <begin position="214"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="18">
        <location>
            <begin position="506"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase I subunit RPA2" evidence="19">
        <location>
            <begin position="613"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="14">
        <location>
            <begin position="722"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="15">
        <location>
            <begin position="1088"/>
            <end position="1152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF06883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OEJ85207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1222" mass="137886" checksum="5FF778B7922DE47B" modified="2017-01-18" version="1">MSSVILPRIANRTADFRTVEREERFKNPPKDKSAFPLLQEAVEPHVESFNELSDSATALGLLNLAVKDLGSRTVYDGSKMVNSQGEVDVTNPDFLGNKLSMKIEQVSLNKPMSNDGVSNALERKIYPSESRQRLTSYKGKLMLKLSWSVNDGEETFSEVRECGYVPIMLQTNRCHLYNLSPYDLVQKREESDELGGYFIVNGIEKLIRMLIVQRRNHPMAIIRPSFANRGASYSQYGIQIRSCRPDQTSQTNVLHYLTDGQVNLRFSWRKNEYLVPIVMILKALTDCSDKEIFDGIVGSDLENSFLTDRLELLLRSFKKKYPTLQNKYQILQYLGDKFRIVFQAPQGMSDHDVGNMVLNRIVLVHLNNYDNLSNSEYKPEKPSDIHNKDKARMIFFMIRKLYALCAGECCPDNPDATQHQEVLLGGFLYGMILKEKMEEYLNNIVLQIQSDLNRGLAVNFKSKKYMSRVFMRINENIGSKMQYFLSTGNLVSQSGLDLQQVSGYTVVAEKINFYRFLSHFRMVHRGSFFAQLKTTTVRKLLPESWGFLCPVHTPDGSPCGLLNHFAHKCKISTKESDVSAIPDLLFQLGVVPSSHILAAGPSMSCVQVNGKIIGWTSHEQAKIVADTLRYWKVESQGTDKIPLDLEIGYVPXSTRGQYPGLFIFGGRQRMMRPVKYLPLDKQDIVGPFEQVYMNVAVTPQEIANNIHSHVEYSPTNILSILANLTPFSDFNQSPRNMYQCQMGKQTMGTPGVALCHRSDNKLYRLQSGQTPIVKANLYDDYGMDNFPNGTNAVVAVISYTCYDMDDAMIINKSADERGFGYGTMYKVEKVDLSQSRGRGDPITQHFGFGDDEWPKEWLNKLDDDGLPYIGTYVEEGDPIVAYFDETIGKTKIKTYHHSEPAYIEEVNLIGDESNKFQELQNISIKYRIRRTPQIGDKFSSRHGQKGVCSRKWPTIDMPFSETGIQPDVIINPHAFPSRMTIGMFVESLAGKAGALHGIAQDATPWNFSEDDTPADYFGDQLAKAGYNYHGNEPLYSGATGEEFRADIYIGVVYYQRLRHMVNDKFQVRSTGPVNSLTMQPVKGRKRGGGIRVGEMERDALIGHGTAYLLQDRLLNCSDYTQTAICRDCGAFLTTQASVPRIGAKASMRCRRCAIKFEDAKKAISKQGDNIYIDDSNIWEDGQGNKFVGGGNTTTVGIPFVLKYLDSELSAMGIRLKYNVAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REQ6</accession>
    <name>A0A1E5REQ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Serine/threonine-protein kinase MEC1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REQ6"/>
    <dbReference type="STRING" id="56408.A0A1E5REQ6"/>
    <dbReference type="InParanoid" id="A0A1E5REQ6"/>
    <dbReference type="OrthoDB" id="381190at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FATC" evidence="1">
        <location>
            <begin position="49"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="81" mass="9373" checksum="5ABEB468FDEA1CD6" modified="2017-01-18" version="1">MMYGRSKSLQELTLKTNQRYFIGRENQTVSDVLKVIRNKMKGIDPIDFVAISVPAQVEALFQEATDEQTLSKMYMGWLAIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REU0</accession>
    <name>A0A1E5REU0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1708</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REU0"/>
    <dbReference type="FunCoup" id="A0A1E5REU0">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REU0"/>
    <dbReference type="InParanoid" id="A0A1E5REU0"/>
    <dbReference type="OrthoDB" id="4005299at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018825">
        <property type="entry name" value="DUF2427"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018827">
        <property type="entry name" value="YTP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31685:SF3">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_6G12730)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31685">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_6G12730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10348">
        <property type="entry name" value="DUF2427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10355">
        <property type="entry name" value="Ytp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184719" evidence="3">
        <location>
            <begin position="28"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="359"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="389"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="491"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="593"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="660"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2427" evidence="4">
        <location>
            <begin position="99"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Protein YTP1-like C-terminal" evidence="5">
        <location>
            <begin position="368"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="289"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ85386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="693" mass="80201" checksum="450B15D905257D34" modified="2017-01-18" version="1">MKLFTKYQKSTLVTGLLFLSVFLKVHAHEGHEEDVDENEEESNHGMDMVMPEESHSTSSSTSTSNSKIIPTAHEQTHMHGMPILEMGKLTPAERLYWEQYNTTTYFTFEHPKVDKTSMWFHVYYIAVLTVFVYPILLGLNNIGNKWYLPLLGLNYIIMWISSFCFYKFHKNLEKNNIQLYPNNIMHQLTCVLLGFFTVHLISAFIRRALDYGDYNESSNNNTDIHTSKYSAYGRLNDLSSYDDVDFEMDGYIAEEQERENGSKSITSPPTTLRNSHDIDNLNKNAKLQSTRSNSNNTLTNNHSETNNDHPNNSFAQMTNKKLEKLQQKRDSMYHKLYSVSLLQKICINFHNLFNFCFHLINYPILMFLFVYCGIGLAVGNIYATPKIRVFNLLAHWIKGGVFIMLGLVSLSRYCGFGKTKSWAWNTTVYTKQDFINRKMNTQGWWRFLPKSGVNMDFIEVFLICFYGTTNVFLEHLASEDGKWTAKDLQHVSIAFMYIGSGACGLLVEYYLNDWKYQYAIKTMKKYYKEEHSTQHNEDDVDSLYSPENVVIANPGFSPNPLPAFTIFWTGVLMSKHAQASQVSTAVHVQWGNLLAYGSFFRIFTILYMFLVPNYTPLKAVRPLTELVTSFCLLCGGLIFMESTDQVIEGMEYRGYTEMFTFNLSVGGVTLLMSWIMLFFILKDWLKKTQSRRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF38</accession>
    <name>A0A1E5RF38_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ino eighty subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RF38">
        <property type="interactions" value="210"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF38"/>
    <dbReference type="InParanoid" id="A0A1E5RF38"/>
    <dbReference type="OrthoDB" id="3971444at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038014">
        <property type="entry name" value="Ies1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37287">
        <property type="entry name" value="INO EIGHTY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37287:SF1">
        <property type="entry name" value="INO EIGHTY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="445"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="445"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="477"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="564"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="579"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="719" mass="82429" checksum="84C231FC3EFB62FD" modified="2017-01-18" version="1">MSMRRVYDPVHDSMHQSQEEDQTPFSEDDVTSPLPNRERTYSRSSANGKSAYKSRRLSIMSLMNDDDAKASSLTENPNAISQPEESQVVNASSSPAEQINTGASDPSKKREPSRYQRHLKKTDGNSFTRSDIQYGFMTELFQNDYCIFTNMFKPKGFTFDCNMAITPLSKEANLGEQEPSGSSSFEVSEEGSILNHFINHDKKETVYDARGWINNDYLTFGQLYVLSLVCSARCSKVLRDKILTDSHIAFAMCILSLLVNIGRLNTTNNFFIEMTSQSRTFHTIPVLQYGINPGSVHLQDTPRIKSILKHLRINKETTTLEDFYQNYTSDASFNVIYLLFAMFETPNIINEKFLAQYVEFETFEDNENEKEKKNVTLFDISDNGDYDSKQRADIFIWLIYIHLEKQLTETQLEESVQKFQTLSXVTGKPSKKFKLSKSVNAIDKDTSQETEYSREQNEQRKEFLAKNNLSRDEEHTLSTNNSPSKQDTLDQDFPKEEEEEDYMSKEESSITDNTSTNDSKKEFSKKNSPKRNSLEPLSPNFSKSDDTAESSQKKRKLNKKVASSLRTEXNGQQIAQSKEAMEDSKVLSKEPVSSKNSKNEKLLAQLIDTEEKRLLRLDPTTHSSLSSTVRNNKDFTQQEFKRQLYHSQVLAGKKRNELGYLKVFQDYEDIPMATVLGIRGKKKKKYQDSVLGFETDMLKAFKVAKSKMLESVAEKLFED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF68</accession>
    <name>A0A1E5RF68_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Myosin light chain 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2020</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF68"/>
    <dbReference type="FunCoup" id="A0A1E5RF68">
        <property type="interactions" value="254"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF68"/>
    <dbReference type="InParanoid" id="A0A1E5RF68"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000003">
        <property type="entry name" value="Calmodulin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="2"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="75"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="143" mass="15970" checksum="B95058C631BF014A" modified="2017-01-18" version="1">MSTTKTYKDIFTLFDKKNEGKITQGQLGDFLRAVGYNPTNALVHELAGTSSSFTLNEIDQLVLENKQVLEATTQASVQDFMKAFQVFDKDGSGKISVGDLKYMLTGLGEKLNEDEVDEVLKGVESDQDGMIDYKKFVEDILKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFG9</accession>
    <name>A0A1E5RFG9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Eukaryotic translation initiation factor 2A</fullName>
            <shortName evidence="8">eIF-2A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2072</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the WD repeat EIF2A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFG9"/>
    <dbReference type="FunCoup" id="A0A1E5RFG9">
        <property type="interactions" value="1222"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFG9"/>
    <dbReference type="InParanoid" id="A0A1E5RFG9"/>
    <dbReference type="OrthoDB" id="2194683at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011387">
        <property type="entry name" value="TIF2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013979">
        <property type="entry name" value="TIF_beta_prop-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13227">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13227:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08662">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017222">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 11" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 8" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Translation initiation factor beta propellor-like" evidence="10">
        <location>
            <begin position="254"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="526"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="538"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="558"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR017222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="628" mass="70105" checksum="E78ABC0FA59CA18B" modified="2017-01-18" version="1">MASQFYAKTSETIGVFQGYPEFDATLNERFFTLDPPKSENAAENDPEHDEEVMFFSQNTRSTASVITADGSLMAVAFPKFIRIFTGESFNNLVVELPVEIATHDIRFSPSGNYLSTWESFNLDGKNPNHKNCKVYYTRDAATTKPLYEYTHKSQSNWSLQFSKTDAYCCKLLSTNELKIIKLDSDEKSKKTAGFDFSQAWSTIKFPYAISQYHISPSEQTPSIVTFIPEKQGKPACIAIWPFMENEVDTPIISKNFFKADSCQLKWNEQGNAVLGLTTTDFDKSNKSYYGENTLYMLSFTGANGKLGSKSIRVPLNKEGPVHDFTWAPNSKEFAVCYGFMPSTTTFFDLRGNIIHSLSPDSKNTIIYSPNNRYVLVAGFGNLKGLVDILDRNDDFKKLSEIDGSNTMTCKWSPGGEFIMMATTSPRLRVDNCIKIWSYSGKLVFVQEYEELLQADWRSSHAGKDPFFEKKETMKIQAHPSVTKYYLDHPSKAPNAKKAKPILIGSWKKKAANRTVVGGANIVNAKPIGATAGVPGTTKKPANSAKNNNNEKKTPPPSSSSSAGGNTSDVTPEQKKIRSLLKKLRAIKALKDKQDSGEKLEDTQVLKIESEPQVLRELKFLGWSEGDKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGF3</accession>
    <name>A0A1E5RGF3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Diphthamide biosynthesis protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g1552</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the first step of diphthamide biosynthesis, the transfer of 3-amino-3-carboxypropyl from S-adenosyl-L-methionine to a histidine residue. Diphthamide is a post-translational modification of histidine which occurs in elongation factor 2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DPH4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ85992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGF3"/>
    <dbReference type="FunCoup" id="A0A1E5RGF3">
        <property type="interactions" value="380"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGF3"/>
    <dbReference type="InParanoid" id="A0A1E5RGF3"/>
    <dbReference type="OrthoDB" id="445556at2759"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.660.10">
        <property type="entry name" value="DPH Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044248">
        <property type="entry name" value="DPH3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007872">
        <property type="entry name" value="DPH_MB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036671">
        <property type="entry name" value="DPH_MB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21454:SF46">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21454">
        <property type="entry name" value="DPH3 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05207">
        <property type="entry name" value="Zn_ribbon_CSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144217">
        <property type="entry name" value="CSL zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51074">
        <property type="entry name" value="DPH_MB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="J" evidence="12">
        <location>
            <begin position="3"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="DPH-type MB" evidence="13">
        <location>
            <begin position="103"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="30"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ85992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="182" mass="20831" checksum="FD04D0149D06940B" modified="2017-01-18" version="1">MATPFEILGVSVDSSLEEIKQQYREKLLGAHPDKWHRQQQRNNATNADQIGSTKKLRQEEQVPSVESIKSAYHVLSDPLRKEEYLKSLATKLQKQGMHSSGDGLDEFSLDDFSMVERAESPDELVDWFMDCPRCTNKNGFHVTEDILEQNGEELPNGMGYQVIIQCSMCSLWIKLNYDFADN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGI9</accession>
    <name>A0A1E5RGI9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein STB2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1371</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ85997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGI9">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGI9"/>
    <dbReference type="InParanoid" id="A0A1E5RGI9"/>
    <dbReference type="OrthoDB" id="19806at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070822">
        <property type="term" value="C:Sin3-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038919">
        <property type="entry name" value="STB2/STB2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059025">
        <property type="entry name" value="STB6_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31011">
        <property type="entry name" value="PROTEIN STB2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31011:SF2">
        <property type="entry name" value="PROTEIN STB2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25995">
        <property type="entry name" value="STB6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="919"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="domain" description="STB6-like N-terminal" evidence="4">
        <location>
            <begin position="11"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="608"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="777"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="635"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="961" mass="110802" checksum="F24E963831799B47" modified="2017-01-18" version="1">MENSIQGNVPFLFADFHLIFQLIKELPEYQYSCLEVEIKGYEIYLAEQWCLDREPTTVIISYTGDPTMTVKCCKVYLPLNPSKWPTILQHYYKFIENCKQLKYINRMGYLFISDIHDLNFDYQNLNLIPVPAGDVRRVWHNFTNNINLRRLHCGGRSSNMLQPPVGAALDKFYQLFKIVKKPTLNTEAIVKVLESEQANKSLTKNXTSTGKTEDFAESHPVYSVVEIVVYIAQISLRFCNLLDIRYVDGFFCHKTERALYKWQKTYGALYFPALIHQTKLLTPSILAALISLLLVCFYKLKIQNCLQQYSAIPDPYIEPRVMYTAIYHFQKKLTSKKVECSKASYLDIALIDKLFQVTENSDANDFSNLKKLVKTTVKDISVTAGINGIGVSMGAGSAMFSLASTDNLTNNIDDLVKIINTFRAVFNTSTVVDTTELKEERAALNKAKNKGSSYATNIDSPTFLTHTNEDGLARALTHMNGNSRKRSKNSIPVSSSTHSCKGSLLGWLWDENNKTMASFFYNDFIWFDYSKHFDTDYEYFFQEQTCNAELCLNQGEQMDRTFGISHXSSSDFKEQDNDYYENLGENSDEKLEKQKIEEFYACVSAKKHGNAKSNGSSSKTSRKGKALGNALDENGMXKEETIDPNKIDEINEKLKKEQEKQCEREKERILYDPEFRKELTRSKSTNDLLLNSEGTYRKASQVLQRTHSFSNVQDYFEDFDRPLSFVRFAIDFKKLNTLKCQTFINDESKLPKKLRFLQTKLLPNVKTKYDDLKYQYENELETKMSIATKQKRQLESVVSKYNYDIRLLERRMRNVSEALEQFELKAHNTKKQLLEAAKVKGILYLEELSSEGIVTDEEIDAIFFSNTNTSTQQLKKKDDQNLAAIKLVSSVGSNSQHNATLNDKIELLRKRQETQFKGFVFSVASLLWIIXKAVYFRIQLYSQDTSKRTRRLAKDRKLDKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGS7</accession>
    <name>A0A1E5RGS7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1427</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGS7">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RGS7"/>
    <dbReference type="OrthoDB" id="3972313at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="448"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="489"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="519" mass="60433" checksum="D626AE5C0CE93B82" modified="2017-01-18" version="1">MVASKHRKSQHAHRSHRNQNEHETQQSEENKNSEQEVYNQLQDSTGSDSIATHSQEHLMTTSEQSAYHKLDKLQYFDFDEHFVHLTYAPPAVKKDRAKLFPHSTRYSYMTSSAFLKKKYSQKAVKIKEFIDCIHCKITTDVNASRIKKFENLQNIDQQLKEKTTLSPSNSPQKDQDYNEKWRKMELFLLLVFLSQRGGPDYWCDQKPVNEEVVNATQQPEPXLTAQATEQSGKAEPSQNDNTKNXSASSSSGKDKRKRKHKTLTEYIIKQNINKSYSEDSPTSSSSDVNYVYSSIWANFMEGLINHYMEEKIIPSVEKEVCFHLYIPMVRIMVLCHQYQMEQLVENPKLANKDLQKENETLMNLPVPAMITNKLEQLADLYTSLNGDEQDFQLDEFVFCAEEYIETVYIPHLIEVLFEHLKLGNEYVWDVLEVLKPFFYYIEETDEDNEXXAPNGKPMNDTAAQARPXHQQAEHNDEVQSTEAKFVTKNPKETKIGNRSDPRVFTLQRIFEVASQEHWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGY8</accession>
    <name>A0A1E5RGY8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RGY8"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="385"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="663" mass="76151" checksum="5699AC963B32C2FC" modified="2017-01-18" version="1">MAILARNPPFKDISNNAVSLNVSNKQQWHENLKRAGITEEQFENPHFIEFLNNYHGATPKTSITTNSNSCLSMNVLNSKQAESSYLPDTSFASRFSESVKSLADHDFTLQDQINEQEGTVSPTQKNDLEKVLQNYSLFGTLDFDIDRKAWKKKLRSIGHKRREKKMMTEPSSFEFRQQLDQLAKKKKDLVQVYQNVLSNLSQWTESQNEMSLTANSVKMLESLQTLLKNDYEYEVNAAKKWNGFAEWLLSVRIREVSLFVSRCNVQDQNQKVVTSQNEMSSDVDFYMSEYEKAKSSLDIDYAAYNKFLTNTGLMMLCNNCNHFDERLLKMLDCNKIAFDQGFAYLKAEYPDLMNKKLQSCTISNKQLLKSLNKVSKDLVSPVYQNLGENENNHDNKQFSNFGQSSSVSTNYESQTSFSKKQNDAKDNYIDDVERSEMIRKKEIEIEKKELNYIKGMAAKSPLTQTIDSYDYNQGNATYMLNDSPMITDFRKSKIPTPFDKFTATRNKKDEMSLTKIQKERQEHNPFNFSDLNSQILSAPEATDYECKQQRFPSLQSHNTTLEKLAGDNEDELKVDITFNTIDGFENNIQVKDFTDDCKTRTDKRDPHLQDMFDSGNITAVVSAAQDXVYDAGNNSIGAIKSLLPSLNPWSLPKKTSQQVDIHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH29</accession>
    <name>A0A1E5RH29_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1432</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH29"/>
    <dbReference type="InParanoid" id="A0A1E5RH29"/>
    <dbReference type="OrthoDB" id="3971561at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23139">
        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="13"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="165"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="72"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="90"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="241"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="5">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ86184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="637" mass="71853" checksum="DB854F1C70851263" modified="2017-01-18" version="1">MDTLENLRAQIMASMGGGAKAPSNTSNTAKDNSRRGDVQQSNRSGGSVSESRGNGSRTSDQSVRSDRRPDNHNLSTDQNRSGSRAFRGQRVSERRDHYENDRETRTTSGSYRGEANNDRXYGRNHSTNQNNYQGQXKYNHRTHDGRDKTHEKVIRGSVVAGQRFESNGARQHTPHAVPDNNDFPKLPKGPAAGNREAFANSGHVSKPAPKRTNNFXGQTRNYYGGKVISGFSGSSQYQRHPSQTXQSMPSXSNTEAAVDWNLVKSIDELRKKYDIANITKWDVPADGFEKVPAQQVKEMQVFSSPDEIIRRNTYFNYKQNMSLDSGSSETNRGRQMQKGFNSSNVNAEIVNNEDVTXEIIPKFYETELNKLLVFNFDPQEYLGKFCESVFVGNDVKALIPPADQWKVXFIKQNDLGNFVVKFSNRFMSDAVLLTHTLLKPKSSPHIVSKPDSYTKDLEKCIKNWAVKGSTTASMNGDDTGSDSTKINFSENSPFFSVVLEFVHYEHFFNPTTTMNSLINKQSTPRAPSCVLALYNCIDPVELKKQEVFDKILKSFENQKNGILEGMVGIEIPRPPLDYIHTNTEMTMNGGLGKIFIKFASTEKSLXVFKELQDWKFQNRRVLVQFYSQIDFDNGIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHB3</accession>
    <name>A0A1E5RHB3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1459</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHB3">
        <property type="interactions" value="525"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHB3"/>
    <dbReference type="InParanoid" id="A0A1E5RHB3"/>
    <dbReference type="OrthoDB" id="504170at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14081">
        <property type="entry name" value="STKc_BRSK1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF110">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="83"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="29"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="395"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="881"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1055"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1308"/>
            <end position="1332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1356"/>
            <end position="1379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1420"/>
            <end position="1542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1555"/>
            <end position="1583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="84"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="404"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="906"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1308"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1320"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1433"/>
            <end position="1449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1457"/>
            <end position="1472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1474"/>
            <end position="1487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1488"/>
            <end position="1508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1529"/>
            <end position="1538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1573"/>
            <end position="1583"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="9">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1686" mass="189035" checksum="A67D428CF392B439" modified="2017-01-18" version="1">MFDALKTPNLNDLAATQCPSKPXLDDYRRNNKVLNSSNXTAAPSTSSSSFMRSASKSEGLRLQRSTQGLRKKKPKNKDTVGPWKLGKTLGKGSSGRVRLAKHMQTGQLAAIKIVPKKHHKRNGSTTVDQHSSRAANNYKDFETDENHGKNHTEEVFEETPIPYGIEREIIIMKLVTHPNIMALYEVWENKSELYLVLEYVDGGELFDYLVSHGKLSESEAVYYFKQIIEGVXYLHKFNICHRDLKPENLLLDKKNKKIKIADFGMAALEVSDKLLQTSCGSPHYASPEIVTGKKYHGSSSDVWSCGIILFALLCGHLPFNDNNVKKLLLKVQSGQYQMPEKLSDEAXDLISKMLVVNPLQRINIEDILYHPLLNKYSRSTKNKSNSNIFKMLRTSDEHDDGETNGNYDISKNNNNNSNQYLSARIGSTKKSQLPIKPEIVALKSKEEIDATILESLQILWHGVSKNTIITNLLKSGVTDEKIFYSLLYQYQVKQHHLNQEQKRLSDALNNAEQQKQAXNKTSIYDSSSTTSELLPALSAXNENQGLENDYENKIQENSNNAISTESVSAEEPSAPLLKPKSQFSSRQSLISRVDSYVKLNKSSSMTRTNSGVRSFVASRSISSKPGLGISGAHAKSPRKPTLLSKSLSNKAISRNGSIPVSPRRFFRSPSSNHYSPNKMFSSQSKRSLYSLTSISKKSINLKNYLINDHYVENEENPLPEYKNEFAILCEQILFLDDTQEATSPNKRNELDTNEETLDLKKSKEIGGSAETLKAVASSFNKEENTNSNINNTTTINHASNKDRNGTLSLGEELQKSVGPMXAQTXDENYFRRLSLDPRLKKSTIQNLTTLLKXNSSIQRRILAGTKTQDVSVETVDERYSGLRNRPLNADVDSDRDNNASRRKSQLPRSTGNNKTGLGMISMPSGALKTTTTFQDLSSYIEAINFGEEKNATGEAEVFDHNTTQNDEQVGNDIPWDQTTIASRIETAEIVSVRKPDLVSLQNSPEQAVPRIDENVFEDLEQGNSSFSSSAYQYSRDNTIDDSDGDLLQKNIQANRKSISQSSDDGSDYDSNVNESSERSKMSIPHRKVASIDSFGYNDVNANIIHTAATVDVRGSLYVNRLHNNDQNEATLRNPEFRLTDTSTSSNIKFDDRDSLAGDATKDSVLNTQLINALNDEMAAKSSNVVNGLPTATSEEIIAKFKLTPDKKNNGFLNFDHSTAQNGVDNTYSFLNFDDTYSKIEHDRQTQFLKFKHSESSLDMAQAFKNLDDDLKXQNKKPELGNKNFAGLVKNEVIGSILEDDYEFSSVDDNSSKDFNNTESQHNEELPDSKKNSRLMEISVTAMNLSKNLVNQNVKDSNALKESASSKHKHTDKNSSHALLNQDDPVAEMIADMNEREKNQNKRVTMLFDNDEDKKFSLKMTNSQDGKPIRKKVSITNLKSRNKSGNTTPISPLFEVDQSTKNEHDTRGFDKSGKNGTNRNDSNNNSASTIFSNGKNKNAKVSFNNSTVTVVPGPGDDVNVQDSVHQNKRNSSNTSTNSTAKRENWFSKMINKLGRRISSTGRAESTNDRYASGSRHNSGRVQNPTILEEKQQHYTSMSFEKLNELATTHLNKYNIVYQPMKQYIVQPNHIKYNCTSSGDGKLYRFQLEIDDNVIVISKKFTSTSEPEDRQQFQEFNKNISNMIRNFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHE3</accession>
    <name>A0A1E5RHE3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1661</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHE3"/>
    <dbReference type="FunCoup" id="A0A1E5RHE3">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHE3"/>
    <dbReference type="OrthoDB" id="4070503at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000011">
        <property type="term" value="P:vacuole inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035293">
        <property type="entry name" value="Vac17"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17321">
        <property type="entry name" value="Vac17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="454"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="537" mass="60159" checksum="43E0270A2BBB56C1" modified="2017-01-18" version="1">MNEQFSSITGSFLLQSQEAIRNLGKCLETLQKECLENNQALQLDQIQLFDKYERFLSNLSSLQLRSEHIKQKLTSTCDDKDNLENSKQERISFLKTNFSSLMSDFAEINTQLQELSTDINNASAKEDIDPAQDLPLEQQHQLSEEAGSSWKEQCWDKEGSPLRQESPSQHETPGSTFSFQPKPLKLLEKTVSEMNNYPQNGAEGHGPYEHKKSPIKKRHSRRRNSNDELRLPSIFNGRNRISIALFQEDDIYSPNNNNNNNTNDDETVIYPMLDPADEVLEETISEGNQSPEKLLDHQSISEDTPGTQLDDFLNNEDLYNTRPALRRYNSHESILSVQKTPLLFHTTGAAASLSIDNQTTSIQFNPGNERKPAFAGGSMLWNACSSTATTAAIASSTIVNSSKELSHSSSRLLLSQISTTSTGPPFNNTKIHHPITRKPAGSGQSFWNQLKQYTAPSAPSPPFSEIGHRKNTNTSPNRSYKLPQHNRKYTNRGQSLTMTNKGSLIYSGPNTAFTKQSVQTEVQSDLLTDALTSNIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHE6</accession>
    <name>A0A1E5RHE6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Bromodomain-containing factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1262</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHE6">
        <property type="interactions" value="589"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHE6"/>
    <dbReference type="InParanoid" id="A0A1E5RHE6"/>
    <dbReference type="OrthoDB" id="784962at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05500">
        <property type="entry name" value="Bromo_BDF1_2_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050935">
        <property type="entry name" value="Bromo_chromatin_reader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027353">
        <property type="entry name" value="NET_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22880:SF225">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN BET-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22880">
        <property type="entry name" value="FALZ-RELATED BROMODOMAIN-CONTAINING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17035">
        <property type="entry name" value="BET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="4">
        <location>
            <begin position="332"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="4">
        <location>
            <begin position="539"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="16"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="154"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="420"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="628"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="717"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="941"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="42"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="154"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="189"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="213"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="260"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="448"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="458"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="476"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="490"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="663"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="719"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="944"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="978"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="5">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="5">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="5">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="5">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ86307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="991" mass="109361" checksum="C9AEC497876564B5" modified="2017-01-18" version="1">MTDYERVMVNMNQQDIATTNGNGTTVXTNTNTTEENTNKGMNANNTSTFENNSTNNTPNNSEDNTTTITTNPNNNNNNNKNNKITPLTPDASNDNDQKMLMDSSAYQKENENIYTSVANRNNIGITDEKDNNNITDHNNDTSIDINNDINNNINNNNINNNDKNNNDDXNNNNXDNXNNNDNENRNDNNDITNNNEQDHNNNNNDNNNNNNNNKDKDNKDQENNESVGEDSELEPLQQATLEEQEAARQENTTTTETIKSEINTNEPTSTSSETNVSVDADADASANANADPNADATVVTAAQPPPPDLTPSKDPLPDHQHKYATTSIRNIKRLKDARPFLVPVDPVALNIPQYFQYITKPVDLSLIEKKLQLKVYSNVEEFYSDFKLMVDNCVRFNGETSGIAIMAKNLFQAFEKHMNNMPSRDLPKKNTASNTSSTSSSGGGGGSSSSSNTTTSTAXQSRSGKSFTNGTSGVESAKAATATSAAPASKRPKRQIHPPKTKDLYYNEISKTNAKPKDKNILQDLNFAKNVVKDLMSKKYININFAFLEPVDPVALNLPTYFDYVKNPMDLGTISKKLANWEYKNLEQVQSDIELVFNNCFAFNPVGTPVNDLGVKLKAIYEKEWMKRPEPLPPTPEPAHQGSGYYYGNDNEDGXYDDNGYGEYDDDNSNGEYSDEEIDEASVTTPAIQFMEQQLVQIQQELKKLRKIEIDKIKNDRRQAKRSMKQKMVKRKGNNGRTNGLKRKRGGAVGMSSGPGNHKRSKSQQLEVVATYEMKKYMAEKLEHLTPNELDNISTFVQANIDLESLPKDEAGEWVFDLNHFPNDLVVTLYNTFFKKYTDFHNGNGVAATPVSSFXGNSAGLNNHLTVGTPVAYGSSVSTPADHHVFYPHANGANATIMGQPVSGNSISPITNPALLNASGRKKSRSDEAEKISQIKQKLEMLNATASPGQSLGTPTSISNLSKGSVPSLHGSDSQSGESDDDMSSDESEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHJ5</accession>
    <name>A0A1E5RHJ5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Synchronized import protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1274</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the nuclear import and ribosome assembly adapter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHJ5">
        <property type="interactions" value="256"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHJ5"/>
    <dbReference type="InParanoid" id="A0A1E5RHJ5"/>
    <dbReference type="OrthoDB" id="288703at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13394">
        <property type="entry name" value="Syo1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052616">
        <property type="entry name" value="SYO1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13347">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13347:SF1">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00049983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="625" mass="71062" checksum="C075DE37118E94C4" modified="2017-01-18" version="1">MGKVKRRRNNNRANPLGSKTTNNKEEAKYVTRINTLMKNLESVVPNERVMALNTITLLCEEEKWRAMFLKQKLVSILLQKLINDSNDEIVVDSTGLLRNICIEEGYDVCTYLWRNDFWVTITSAIGKITKSLKALQNNSTNATYNDKNGKEGKRLLFDYADNLISCINALTNGSEDILNDILENKLSILLEFVVECLQYISSSTESNLPVNLYNSLLDLIYDFSSESADFVQALGSHAYLSTFIAELFNKVNDKSNTFNEQEKLLGNDLTKILVEGIRLQLCDMELSYTDANVIAQQCIEIITKIDLHQIKSDLSSTEEEDLVNGSIKQENVGKKLKEITKRKENAMMKVQAVEIGVDIITAVLEIVGSNIEQQQXQNKIKHSTNSIEILEQTFNILPQFLHEVFTVLELVQTRVFICWNNFLWVLLSISGQGVFAMDASASWKLLVQDMNAETANENDVSFQITKYGCLWALLKSDCPQFLQETSINALELAEKLINLYKQNESEELHQRLIGCLTXLSKQSVELNSLIGDFFVLSLLSTDEKSARCLCDVVNSIFEIYADKSYAYDKPVFQDRQYLNQLSTVILPRLKTVFKLVDRNKDAGLKDFCTGTFATLEKFIHYKKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHR9</accession>
    <name>A0A1E5RHR9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1391</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHR9">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHR9"/>
    <dbReference type="OrthoDB" id="4053447at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ86416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="189" mass="21506" checksum="36513D7ECC2537B7" modified="2017-01-18" version="1">MDELIKQLLVGQGAIEAQGSQEETSGCCGKKVMIFALGESARQYFHQSPIYGSMGIASSVLAMLNTHNYIDLLFLEKLQYLFMYLTKFEATELPKLDYNHIVIFGLEELVLTSKSANANNSNDTELKLHGNKLDVDQIRICNLIFSIAYKTKRKHXLTITIVPCSTEEHFDTCIDEMNAIEXYWKKLYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHU5</accession>
    <name>A0A1E5RHU5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]</fullName>
            <ecNumber evidence="16">1.3.1.90</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">tRNA-dihydrouridine synthase 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AWRI3579_g1255</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>5,6-dihydrouridine(20a) in tRNA + NAD(+) = uridine(20a) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53348"/>
            <dbReference type="Rhea" id="RHEA-COMP:13535"/>
            <dbReference type="Rhea" id="RHEA-COMP:13536"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.90"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:53350"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>5,6-dihydrouridine(20a) in tRNA + NADP(+) = uridine(20a) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53344"/>
            <dbReference type="Rhea" id="RHEA-COMP:13535"/>
            <dbReference type="Rhea" id="RHEA-COMP:13536"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.90"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:53346"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>5,6-dihydrouridine(20b) in tRNA + NAD(+) = uridine(20b) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53352"/>
            <dbReference type="Rhea" id="RHEA-COMP:13537"/>
            <dbReference type="Rhea" id="RHEA-COMP:13538"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.90"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:53354"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>5,6-dihydrouridine(20b) in tRNA + NADP(+) = uridine(20b) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53356"/>
            <dbReference type="Rhea" id="RHEA-COMP:13537"/>
            <dbReference type="Rhea" id="RHEA-COMP:13538"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.90"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:53358"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 5,6-dihydrouridine in mRNA + NAD(+) = a uridine in mRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69851"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="9">
            <dbReference type="Rhea" id="RHEA:69853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 5,6-dihydrouridine in mRNA + NADP(+) = a uridine in mRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69855"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:69857"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the Dus family. Dus4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.90" evidence="16"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHU5"/>
    <dbReference type="FunCoup" id="A0A1E5RHU5">
        <property type="interactions" value="151"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHU5"/>
    <dbReference type="InParanoid" id="A0A1E5RHU5"/>
    <dbReference type="OrthoDB" id="9977870at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106414">
        <property type="term" value="F:mRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102266">
        <property type="term" value="F:tRNA-dihydrouridine20a synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102267">
        <property type="term" value="F:tRNA-dihydrouridine20b synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000159">
        <property type="entry name" value="tRNA-dihydrouridine synthase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082">
        <property type="entry name" value="TRNA-DIHYDROURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082:SF31">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(20A_20B) SYNTHASE [NAD(P)+]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="19">
        <location>
            <begin position="58"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00060741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00066483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00071722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00078338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OEJ86446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="365" mass="41964" checksum="28CB5EE46E7E42F5" modified="2017-01-18" version="1">MINSRSHTAKTRKQKRYEAKAVPLYPMSFCRITPHTTKRQTIDPLPLIKNSKGFTKIIGPMVRYSKLPFRATCRHYNCDIVYTPMILAREFVRNENARVSDFATNNSDTPVIVQVGVNNSTDLLRFVELVKDYCDGVGINCGCPIKEQVREGIGSALIYNPDVLCEMVRSVKQKYGDSVRLETKIRIHEDLDNTVDLCRRLHREGVDWITVHGRTRHTRSSQPCNFEAIKYIRERVPQDLPIIANGDCFTMKDFKEIIDKTNVQGVMAVRGALANPALFSGYDKCPWSCIEWIWHYAMEYGTHFPLIQHHLWVMMENAGLGKRELKEIIDTRSIIELLELFDSKFVLKRKGEEGFATGTAIPYRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHX3</accession>
    <name>A0A1E5RHX3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GTP cyclohydrolase 1</fullName>
            <ecNumber evidence="3">3.5.4.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">GTP cyclohydrolase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g1035</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">GTP cyclohydrolase 1 is the first enzyme in the biosynthetic pathway leading to folic acid.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GTP cyclohydrolase I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.16" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHX3"/>
    <dbReference type="STRING" id="56408.A0A1E5RHX3"/>
    <dbReference type="InParanoid" id="A0A1E5RHX3"/>
    <dbReference type="OrthoDB" id="3971051at2759"/>
    <dbReference type="UniPathway" id="UPA00848">
        <property type="reaction ID" value="UER00151"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003934">
        <property type="term" value="F:GTP cyclohydrolase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00642">
        <property type="entry name" value="GTP_cyclohydro1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.286.10:FF:000003">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1130.10:FF:000012">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.286.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00223">
        <property type="entry name" value="FolE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043134">
        <property type="entry name" value="GTP-CH-I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001474">
        <property type="entry name" value="GTP_CycHdrlase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018234">
        <property type="entry name" value="GTP_CycHdrlase_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020602">
        <property type="entry name" value="GTP_CycHdrlase_I_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00063">
        <property type="entry name" value="folE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006825">
        <property type="entry name" value="PRK09347.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006826">
        <property type="entry name" value="PRK09347.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109:SF7">
        <property type="entry name" value="GTP CYCLOHYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109">
        <property type="entry name" value="GTP CYCLOHYDROLASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01227">
        <property type="entry name" value="GTP_cyclohydroI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00859">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00860">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="8" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GTP cyclohydrolase I" evidence="13">
        <location>
            <begin position="349"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="248"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="36"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="300"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ86476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="531" mass="58042" checksum="419AEEF3B1887089" modified="2017-01-18" version="1">MTTRRDSLGINDDDLPPSYDEVLRDEQIRLGTALPSSSSNTTGNSMTNSNNVHSTNSNGSGSANPALPPRRESVRPPARPPRPSAATSYNEYHSSDSIYGNSTAGTGASESTDGLNGLPWAYPSGFRCSKCGNSGYIHKRSNKRCKDCWRDFGPGSAYAKIQSSSGGNAASKLPWVYPRGFYCRKCANTGIKLKNGRSCKDCWKKFAPMSNSARPDATILLGNAYPGSNGVPYSAPYSRPSYGPSYGPSYGPPSSTGFAPSPMFRPPAGNSPGLVVQPGDPRLGGCTEPNAMNHKELTERTGTPLNTKSAKPYTLSPPVESDGLSWPTPGARTRCVETEEQEKQRVNRIAAAMKTILSELGEDVDREGLLDTPGRYARAMLYFTKGYQTNIMEDVINNAVFEEDHDEMVIVRDIEIYSLCEHHLVPFFGKCHIAYIPNKKVLGLSKLARLAEMYSRRLQVQERLTKQIAMALSEILKPRGVAVVIEASHMCMVSRGIQKSGSSTTTSCMLGYFRESHKTREEFLTLLNRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHZ2</accession>
    <name>A0A1E5RHZ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mannose-1-phosphate guanylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1286</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in cell wall synthesis where it is required for glycosylation. Involved in cell cycle progression through cell-size checkpoint.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>alpha-D-mannose 1-phosphate + GTP + H(+) = GDP-alpha-D-mannose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="EC" id="2.7.7.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.13" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHZ2"/>
    <dbReference type="FunCoup" id="A0A1E5RHZ2">
        <property type="interactions" value="1483"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHZ2"/>
    <dbReference type="InParanoid" id="A0A1E5RHZ2"/>
    <dbReference type="OrthoDB" id="1733332at2759"/>
    <dbReference type="UniPathway" id="UPA00126">
        <property type="reaction ID" value="UER00930"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004475">
        <property type="term" value="F:mannose-1-phosphate guanylyltransferase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06425">
        <property type="entry name" value="M1P_guanylylT_B_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000013">
        <property type="entry name" value="mannose-1-phosphate guanyltransferase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045233">
        <property type="entry name" value="GMPPB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="10">
        <location>
            <begin position="2"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="11">
        <location>
            <begin position="251"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ86512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="361" mass="39589" checksum="127694840D3FAABB" modified="2017-01-18" version="1">MKGLILVGGYGTRLRPLTLTVPKPLVEFGNRPMILHQIEALAAAGVTDIVLAVNYRPEVMVETLKKYEEEYGVSITFSVETEPLGTAGPLKLAEKVLKKDDSPFFVLNSDVICDYPFQELADFHKNHGGKGTIVATKVDEPSKYGVIVHDIATPNLIDRFVEKPVEFVGNRINAGLYILNPEVIDLIDLKPTSIEKETFPILVEDKSLYSFDLEGYWMDVGQPKDFLAGTVLYLNSLSKRLPERLSKEKYVVGNVLIDPSAKIAPTAKIGPDVVIGPNVVIGDGARIARSVILSNSHIKDHALVKNTIVGWNCTVGKWARTDGTTVLGDDVEVKDEIYVNGGKVLPHKTIGANVEKESIIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI36</accession>
    <name>A0A1E5RI36_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1294</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI36"/>
    <dbReference type="FunCoup" id="A0A1E5RI36">
        <property type="interactions" value="102"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RI36"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="189" mass="20625" checksum="803002A4A7BC1B19" modified="2017-01-18" version="1">MSTTTTSTAPTNWSAAIKSNIPNTKITGKTVHGSTNAGVLHQSAKQTKKTKSSTNNSNSGIADTNTRTTTNHENKDTFKRPVSANGHKANSPSSPGSAHHQTKPYNGDEIRSFLRNKMDYYLKKQKTSNSVTVHSPYMHTGSSSTGDSFFADNDNEDDWGTAGSKKKNKKNKKYVILNDVGRFLQNGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI80</accession>
    <name>A0A1E5RI80_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Prefoldin subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI80"/>
    <dbReference type="FunCoup" id="A0A1E5RI80">
        <property type="interactions" value="101"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI80"/>
    <dbReference type="InParanoid" id="A0A1E5RI80"/>
    <dbReference type="OrthoDB" id="2015447at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="120" mass="13799" checksum="5AAB84A478046AB5" modified="2017-01-18" version="1">MSQPPIQEMVSSLRNSRTQLSFVDQQLTQLKQQELVLKNTIEEVNSLPLSDASDDSGSTKGKVWRSCGRGFVLQDKKVYLKDLKQNNDTLNEHMRALSIKKEYLTTTIEKTVENMKKHMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RII2</accession>
    <name>A0A1E5RII2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Aconitate hydratase, mitochondrial</fullName>
            <shortName evidence="9">Aconitase</shortName>
            <ecNumber evidence="9">4.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1532</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.-" evidence="9"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RII2">
        <property type="interactions" value="276"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RII2"/>
    <dbReference type="InParanoid" id="A0A1E5RII2"/>
    <dbReference type="OrthoDB" id="2224430at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000002">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000003">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000004">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1060.10:FF:000001">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1060.10">
        <property type="entry name" value="Aconitase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050926">
        <property type="entry name" value="Aconitase/IPM_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015932">
        <property type="entry name" value="Aconitase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006248">
        <property type="entry name" value="Aconitase_mito-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01340">
        <property type="entry name" value="aconitase_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005558">
        <property type="entry name" value="PRK07229.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43160">
        <property type="entry name" value="ACONITATE HYDRATASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43160:SF2">
        <property type="entry name" value="HOMOCITRATE DEHYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="10">
        <location>
            <begin position="71"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="11">
        <location>
            <begin position="578"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ86393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="780" mass="85186" checksum="8D0A6FD18668D6B6" modified="2017-01-18" version="1">MLSRSRAQSRATFATVANAINKKIPPYQKLSSNLELINKITGNEPLTLAEKILYSHLNNVEETFAKTNKVQELRGNEYLKLMPDRVAMQDASAQMAMLQFMTTNLPETAVPASIHCDHLIVGKDGELKDLQSSIATNKEVFDFLQSCADKYGIQFWGPGSGIIHQIVLENFSAPGLMMLGTDSHTPNAGGLGAIAIGVGGADAVDALTGTPWELKAPKVLGVKLSGQLHKWSSPKDVITHLAGLLTVRGGTGYIIEYFGEGVESLSCTGMATICNMGAEVGATTSTFPYQKAHRDYLVATNRAVVAEQADLVKNSYLKADEHCQYDKVIEINLNTLEPSVNGPFTPDLSTPISQYGAKVAQEKWPSKISAGLIGSCTNSSYQDMTRAADLVKQANAVGLKPKIPFFVTPGSEQIRATLERDGILDTFSKSGATVLANACGPCIGQWDRQDISKKSQQTNSIFTSFNRNFRARNDGNRNTMNFLTSPELVTAMTYSGDANFNPITDSIKLPNGENFKFQPPKGQELPPSGFTAGRKEFYPDLDPKADPSVSVNVSPTSDRLQLLEPFSKWNGKELKTTVLLKVEGKCTTDHISAAGVWLKYKGHLENISNNTLIGAQNKETGEVNKAYDLDGTQYTIPDLMIKWKKDKRPWIVVAEHNYGEGSAREHAALSPRFLGGEIILVKSFARIHETNLKKQGMLPLTFANEQDYDKIAAGDVMETVGLVELVAKKGDNGGFLDVKVTKPSGETFIIKXKHTMSKDQVDFFNSGSAINYIGELRRQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RJ84</accession>
    <name>A0A1E5RJ84_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cell wall synthesis protein KRE9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1505</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in cell wall beta(1-&gt;6) glucan synthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KRE9/KNH1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RJ84"/>
    <dbReference type="FunCoup" id="A0A1E5RJ84">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RJ84"/>
    <dbReference type="InParanoid" id="A0A1E5RJ84"/>
    <dbReference type="OrthoDB" id="2432613at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006078">
        <property type="term" value="P:(1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042546">
        <property type="term" value="P:cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045328">
        <property type="entry name" value="Kre9/Knh1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018466">
        <property type="entry name" value="Kre9/Knh1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008659">
        <property type="entry name" value="Kre9/Knh1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28154">
        <property type="entry name" value="CELL WALL SYNTHESIS PROTEIN KNH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28154:SF1">
        <property type="entry name" value="CELL WALL SYNTHESIS PROTEIN KNH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10342">
        <property type="entry name" value="Kre9_KNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05390">
        <property type="entry name" value="Kre9_KNH1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184790" evidence="5">
        <location>
            <begin position="20"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Yeast cell wall synthesis Kre9/Knh1-like N-terminal" evidence="7">
        <location>
            <begin position="25"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Yeast cell wall synthesis Kre9/Knh1 C-terminal" evidence="6">
        <location>
            <begin position="182"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="307" mass="33636" checksum="39558CB9B2A01B84" modified="2017-01-18" version="1">MIPILTIFLLLISSQLIECSINVIKPQKGASFDASSSSSSVSVTVEWVDDDTFPTYSDLSSVTFTLITGENTDMNALKTLASKVSKDKFTKTESSGTDHYTYDVEISNDLVGGNGQYFIQLYAVYDGIGAVAAYSPRFYLEDMSGSVTTYTYSYSTQPSMGYYITNNPYETTTAATTTYDTSASFTVPYVSQTGPYRFAPMQQQPNTTVTATTWTRRFPTSAVTYYTTINTKSLYHVTTITPGWSYTLTSDINYQTPDSFPSENGGWYNPSKRMSLTTRKLNYSAHSTSTTSSSSSTASSTTAASTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN23</accession>
    <name>A0A1E5RN23_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative vacuolar membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g718</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN23"/>
    <dbReference type="FunCoup" id="A0A1E5RN23">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN23"/>
    <dbReference type="InParanoid" id="A0A1E5RN23"/>
    <dbReference type="OrthoDB" id="164921at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019431">
        <property type="entry name" value="DUF2417"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10329">
        <property type="entry name" value="DUF2417"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="300"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="666" mass="76519" checksum="4CE0482965A3B32E" modified="2017-01-18" version="1">MSTEHQPLLGSDTDPSSNLGTSSRYLGNNEQQERYGEIPGHFDAENNPNMNDSYEETMKYTRFNHQSKILLNIVLFINALWLVFTFISDFFFDMNLLFKHGNRVSSFIDLNLITVAIIANILSLIFNYMEYYSVIDEKLNFINSLIILLNFFLFLFVGYTRERLGAVGKWTFIWCFLSFFISGMIDYQLSLYIKPLIGFFERGQGTQEGISIAGPTPNNQGVVVDSQVDSIDPVEHPNGRYARSPTPPSVPSDQDHRAEESHPLSKRQQKKSNKRKRKETGVRHTLTEWSVISLRNGLKILLMVWLVLFTLNNIIYLIDVHRIMSHIDEKTKPVDSKSVDVLTPLVQASGVTTDSMSTDYSTTIDGFFWNDLDSPSYKLHVTCYGNLSDYNEPLVIYEHGSADTAYTSGRWIKDMHNFKKLDKFCTYERPGYGISDSAPSPVSIGLITEAFKDVLTDQILPQFTKKKTDITVVGYDIGGLYAQVLCSKLTQCNKLMLIESWHEDLLAYKTWLPKDSFDHRTLNTLGKWNNFKIWFYGAFESALGLPLMKSLFINKSGSLDRISGDWMKYQGKYTRTRFQETLTGGLLSYNDVVESKKNIFERVDLSVVSSKAMIEKNTRWGDWQRSLAKLSKNVREWEIIDSNHYLYDSTNGKRILQDVLHRLVFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN28</accession>
    <name>A0A1E5RN28_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Signal transduction protein MDG1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g657</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRP1/MDG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN28"/>
    <dbReference type="FunCoup" id="A0A1E5RN28">
        <property type="interactions" value="77"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN28"/>
    <dbReference type="InParanoid" id="A0A1E5RN28"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050827">
        <property type="entry name" value="CRP1_MDG1_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343:SF81">
        <property type="entry name" value="CRUCIFORM DNA-RECOGNIZING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-activated protein kinase glycogen-binding" evidence="4">
        <location>
            <begin position="5"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="106"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="318"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="363"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="433"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="514"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="118"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="318"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="433"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="514"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="535"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ88289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="594" mass="65651" checksum="9434A1CDDB0D762A" modified="2017-01-18" version="1">MSNTVTFIYPAGPNEVIVTGDFDLWRQSLPMVKQIDGTFSLTMPIPVKSLKDGNRLYFKYVVDGEWVTSSDYPKASSTEDASIVNNYIEIDEESKRSSSFIPESVLPMSASSNNLNKQNKRKMKIKRKVRKNKKTGEKTILSEETEVYDGNNGSDSSAIITEQEVSTNENTPFEKTQPTTKTTTEALGKTDDAVVDETLQNDTFAEAAKAKASLPAEPVFTVQPIVPETEQSKPLAGETGPVIPKDADNIKEFNEVRDVDQEELNARLNKELKDKEELSGKTNIKSEQVDAPAAVGKMTSLAGEPGPVIPENPSEIKEFSEIRDEDKHPQPTETKYASLAGEPGPVIPENPSEIKEFSEIRKEAETAKTETAPNEENQEFHIQPIVPEVEKVQPLVGEPGPVIPQNAEDIKEFNEVRDVDQEELNTKLNKELKDKEAVVETPKEAETAKTETALNEENQEFHIQPIVPEVEKAQPLVGEPGPVIPQNAENIKEFNEVRDLDQEELNTKLNKELKDKEAVVETPKKAETPKDTEPTVTATKKTEPITTAAKKTKTAKTTPKPAAVKPTPKSKVTAKKPIPATAQKKEKSECCVIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNM1</accession>
    <name>A0A1E5RNM1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fatty acid synthase subunit alpha</fullName>
            <ecNumber evidence="8">2.3.1.86</ecNumber>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="8">3-oxoacyl-[acyl-carrier-protein] reductase</fullName>
                <ecNumber evidence="8">1.1.1.100</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="8">3-oxoacyl-[acyl-carrier-protein] synthase</fullName>
                <ecNumber evidence="8">2.3.1.41</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g814</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17397"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="1.1.1.100"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>a fatty acyl-[ACP] + malonyl-[ACP] + H(+) = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.41"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the thiolase-like superfamily. Fungal fatty acid synthetase subunit alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.86" evidence="8"/>
    <dbReference type="EC" id="1.1.1.100" evidence="8"/>
    <dbReference type="EC" id="2.3.1.41" evidence="8"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNM1"/>
    <dbReference type="InParanoid" id="A0A1E5RNM1"/>
    <dbReference type="OrthoDB" id="4251012at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005835">
        <property type="term" value="C:fatty acid synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004321">
        <property type="term" value="F:fatty-acyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042759">
        <property type="term" value="P:long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00828">
        <property type="entry name" value="elong_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.25.70:FF:000001">
        <property type="entry name" value="Fatty acid synthase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047224">
        <property type="entry name" value="FAS_alpha_su_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026025">
        <property type="entry name" value="FAS_alpha_yeast"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041550">
        <property type="entry name" value="FASI_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050830">
        <property type="entry name" value="Fungal_FAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10982:SF21">
        <property type="entry name" value="FATTY ACID SYNTHASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10982">
        <property type="entry name" value="MALONYL COA-ACYL CARRIER PROTEIN TRANSACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18314">
        <property type="entry name" value="FAS_I_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000454">
        <property type="entry name" value="FAS_yeast_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="2 8" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184818" description="Fatty acid synthase subunit alpha" evidence="10">
        <location>
            <begin position="26"/>
            <end position="1577"/>
        </location>
    </feature>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="11">
        <location>
            <begin position="910"/>
            <end position="1416"/>
        </location>
    </feature>
    <feature type="active site" description="For beta-ketoacyl synthase activity" evidence="9">
        <location>
            <position position="1094"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000454-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ88491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1577" mass="177396" checksum="C1E9C4D85E884BEC" modified="2017-01-18" version="1">MNPENRLYLAHTLLIELLAYQFASPVRWIETQDVILNEFEAERIIEVGPQPILKNMFNRTLSQSYDKECSNARAGRFVKCVVNDLDSIYYDHVSESKAKESNAENNSNSDLATLDDLHIDIIPKNPDFSSTQHAGAKEFNTETAVAYFECLNNEMVARDTTKAKKIIESADIGGNNPQIHPFLKNRDVSNNLQLFSHIQTLNREIESLNSRTAKIENEFGTQNVKNAISEVFQPNRIRCYDSYWNWNRFEVYKFCMSTDHSRDIDTKRLQLLLANEATTENDFTGLNEILKFNNRSLQIDDNQKFVAMVNRFKYVQQLVVAQKNNFSVCGNEQSSSIDTISARLEHDMTSYYKIEAELFQVYSKIMAYASESSSEQSLVLSEGAITEGFQSVYEQLLVFLRNSNQINPYFRNIVDQALFSLNKANINDLVFEDISAYETEFEPINDSACSSRSSSLFSTDGDDHNIMDKAHRCKEIPSVNGDDGISYELPMLKGVIPSINIKSWSADSKNWSYNKNLTHQYLNKLINTLELDFMKNKKVLLAVDQISNFAVQIIESLLKNGSQIFVIQSCQNTSSVSQFAKTINTIYKNNSIDTSKLSIIPVNIKSKTDCENFVRHYYYDMNTDFSFFLPLNCFGKNTEISTNFKNVQHIIAKVIECKKKLGNETSPLHTLLPLDDPLFASFLQSFELEDQREKKSFVSYMGCKFNLVDKSNGPSSEEMVGNFLSLMTPRLVSLCQESTLCSELDIASEVYSPFAQMSINNAKNVDEKGFSNKFCVEPKVSNNLIKPNFESFETLQNEYNGRSLQGLLDLESVVVVTGFGEVGPYGHSKTRWEIESKGELSVEGCLEMAWLMGYIDYDKKLKVWVDMETRKPIDDSSLKEVYEERVLGNSGIRIVDPSLFEGYNPQDKKTLLQEIILTHNLEPIEMSAELAQQYLLEHKDKVFVEEVENGQCMVTFLKGCSIMVPKALSFDRFVAGQVPTGWDPKKYGLSQDVIDQVDRVTLFALVATAEALITSGIIDPFELYQYVHVSEVGNCCGSGLGGLNSHKKMQRGRMMDDDVQNDILQETFLNVMPAWINMLLLGSSGPVKTPVGACATAIESLEVGTETILSGKAQFVLVGGHDDYGEETAYEFANMGATSNSQKENDMGRAPKEMCRPMTSTRAGFMESQGSGIQVLTTAKLAVQMGLPIYGVVGMVSLAADKIGKSLPAPGQGILTSAKQVTSKLAPKKLDIGYRKLQIEKSFQNLFHHYGVTEETIQTFRESKKYWGTSFFKNDPKIAPIVGALSMFGLTIDDIGASSCHGTSTKANDKNESNIINEIMKHLGRSKGNLLPIVSQKSITGHPKAAAGAWMINGALQMFHDSTIPGGSNYDNIDDYFKNFEYLVYPAESVKVPKLKAVSVSAFGFGQKGAFAILINPNYVLSSLNKVEYEKYTQKATKRHCAAQRYYQSAILSNSLIKEKHESPYSNEVDTYLQPLIRAGKENLIDNNATNEYDMGRPDEYRLHEHLLQRWEVNKSECDKPSWLQKCLSEYFPAHNISVIPGPNELYRMTINGDLAQDMVVGCSVNDQKTVFKLYKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNQ1</accession>
    <name>A0A1E5RNQ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Altered inheritance of mitochondria protein 23, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g971</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AIM23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNQ1"/>
    <dbReference type="FunCoup" id="A0A1E5RNQ1">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RNQ1"/>
    <dbReference type="OrthoDB" id="3996489at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029427">
        <property type="entry name" value="AIM23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14877">
        <property type="entry name" value="mIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="29"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="103"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="333"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="439"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="335"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="458" mass="51423" checksum="677C63634F27ABEF" modified="2017-01-18" version="1">MLRSANKSVLIHTPSFKLLRGTNLLIQDRSYNNNNNNNNNNNNNSNSNANYARGALNNKKAPSSSADKWNLDLGGLGNKPSLGSSKNSTSDLLTAFINKAKNARMRQGTTTSNKAHFKGNKQNRYGKNQDKKSNYKQKKKVVRFSTGSEKQQAAATTVCEKVFSMSPQGNIKAVDKDTGSLIETKLLDFIKGIDLTKEGIVIANIFTEGESKIPLLKIVETRLALKIYSQELAEKKNAELAQLGLKKYASSATGASSSSSASDKDSSLKQIQVSWQISQTDLCNQKINEIINQLKKGFKVNLYIDTKKNLDYHRWLEKFEGLDRPMKIDEGLESVQDSSNEVISSSETTQEDVESAENVKATDGKEETFSKLRPREVSARQAIVDSLFEMVEEHSLTPEIYGEVTKRVLIKLKPRAIDKKIETKKKEDEKRAFKEEKKRLRQEKLMKRTAKKQGFLQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNR8</accession>
    <name>A0A1E5RNR8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g646</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNR8"/>
    <dbReference type="InParanoid" id="A0A1E5RNR8"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="400" mass="45856" checksum="06882C517D2C7850" modified="2017-01-18" version="1">MSESIENPNNKTSAYQKDTIINTKNTNNECPFGPLKMLVFTALLVISFIVYYLYFGMILDTVLKTIYPNYNSENIKSWNNPKMSGSTRSLEFSYICLILSSAMHIVCSGKFKTTNKTHLNVTDNNFRNCSNLQKLFTKMRKLECCKYIPTYFGISLLMTNLYSNYTRLSNLQVLDHQLNAKLDLARTNHINYLNKMEAASFGMMLTEMKHRNYTYNSHNAHGDNAHGDNAHGDNSHGDNSHGEKDMKNIKRDPFQLYNSYAAFECSMNRNSERIVKDNDLENQLEFQQYHTLLKGMENLEYYKQNCQDQCFLSLVVLAGYGTIQGLCYFLKDQETDENSVSTDVNDSSCNSNSECNCDCITKLTMENSKYSNDFAATESDEENDLDMDHCLNKDMEAGFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNS1</accession>
    <name>A0A1E5RNS1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Rho-GTPase-activating protein LRG1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g575</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNS1"/>
    <dbReference type="FunCoup" id="A0A1E5RNS1">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNS1"/>
    <dbReference type="InParanoid" id="A0A1E5RNS1"/>
    <dbReference type="OrthoDB" id="20689at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030695">
        <property type="term" value="F:GTPase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08368">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09391">
        <property type="entry name" value="LIM1_Lrg1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24215">
        <property type="entry name" value="RHO-GTPASE-ACTIVATING PROTEIN LRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24215:SF10">
        <property type="entry name" value="RHO-GTPASE-ACTIVATING PROTEIN LRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="8">
        <location>
            <begin position="10"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="8">
        <location>
            <begin position="422"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="9">
        <location>
            <begin position="781"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="282"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="282"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ88532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1051" mass="120308" checksum="D929E37F300AD06C" modified="2017-01-18" version="1">MTSTEQDTVNNCPKCKKPITDQVIKALGKIYHQDCFTCYDCGTICKLKYFPYKLPTTGELVPLCQLDYFKRNKLLCYVCSQPLRGTFFNAFGRLYDEEHFCCKICGEQCSMQECFNYKDDLYCKYHYLKYFSKRCKGCQYPITDQHFEFPRGDKIYRWHPECYGIHRYWHIDLQPDCLGLPLLLPNSYVKDVQMRDTNPTPAEMNRCINISDNLTCKTWSTLYKFEEETAGCISDMLQYLTSEDKTKGVKATALFVLKVECLFKALDSLQSINDYRVSSSTLSSYSAGKSGKNASSSEEVNGLDAPESSVDPTESIAQKYKKFPKQLSSKIMIYLQLLRKSTLQSDSNEVTVSNVMSIINGLSHYLKLLIRYGLYNALEYNKRSHSTNALVKFLRETNKNLNYPASSNPFDFILDVPIDSTDCCSSCNKYIQQDCIKYQENKRWHIQCFNCSDCGKLISKYDILDATYNKKTQKVFCPLCSMNDPESHPGFKPVTKLLQLIYLLKVALIKTKTVMDIQLKSQMTPSMESRANSISKEQSYIRTVNDIKTLRAKRNSLKITRDNSTARLNSMTRNNFIQSSNSASKLPADKSAIEEFNNSKALTLDDISRIVAVEQARELSKRRDFKQQTSSADELSKTLNSLSVSENNVPPSSPTTALFATHKPGLLKYNSTNDINKNTKVSNKGFRHFNKMNAVSSVSNTSGVYYSDLSIDHLYKLQLIALSILKSSNFPIAKMEDILVMLKKHSQPQLVNPSSSAKFWKSSKTIKRLKQSTEQNRLFGLPLDVLCEAAGVDSDLGRGSNRVRIPLLLDELISALKQMDMSVEGLFRKNGNIKKLRMFTEEVNETPVSVPDLSKENSVQLSALLKKFFRDLPESLLTGELYQNWLNLPTKSEERKMDFVLFYTLLPKYHRNVSEVLLSFLNWVSTFANIENPAQQSGSKMDIHNLATVFAPNVLYQEKPHSDGENDKDRFGDYTLDEELMKRRMENGVDGDYVLVIEVVKYLILHIDEICMVPMFLMKLLNRIINEKLSTYEQIQEFIANNYSKIDFSEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP20</accession>
    <name>A0A1E5RP20_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Serine/threonine-protein kinase RIO2</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Serine/threonine-protein kinase rio2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g612</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP20"/>
    <dbReference type="FunCoup" id="A0A1E5RP20">
        <property type="interactions" value="1296"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP20"/>
    <dbReference type="InParanoid" id="A0A1E5RP20"/>
    <dbReference type="OrthoDB" id="10258631at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05144">
        <property type="entry name" value="RIO2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000053">
        <property type="entry name" value="Serine/threonine-protein kinase RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000566">
        <property type="entry name" value="Serine/threonine-protein kinase rio2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000052">
        <property type="entry name" value="Serine/threonine-protein kinase RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030484">
        <property type="entry name" value="Rio2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015285">
        <property type="entry name" value="RIO2_wHTH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45852">
        <property type="entry name" value="SER/THR-PROTEIN KINASE RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45852:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09202">
        <property type="entry name" value="Rio2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RIO kinase" evidence="16">
        <location>
            <begin position="66"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="356"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="356"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="384"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00068837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ88619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="426" mass="49006" checksum="E983A18AD0C40856" modified="2017-01-18" version="1">MKLDTSHMRYLTSEDFKVLQGVEQGSLNHECVPTSLIHRLSGMRSISGTNRSLGDLAKLKLVSRLRNVKYDGFRLTYNGIDYLALKALLNRESVYSTGAMIGIGKESDIYKVSDKHGVEKVMKLHRLGRTSFHTIKNNRDYLKKNSFNTNWMHLSKIAASKEYEFLGLLYTNGFNVPQPFDCSRHAILMEYIKGFPMRRLRKHKNLPKLYSDLMNFIVKLANNGLIHCDYNEFNIMIKDNHNEANENDLGFIVIDFPQAVSIEHVNADYYFKRDVDCIRRFFKKKLKYEPKADSTMLDTDGYGDGYRYPYPVFHRDVKRINNLDELVHASGFSKKHPGDRDLELAVESMRGNIAEEAYDSAEEYSDNSEEDVEETSEDVYSSDYDSEGDDDSQDGLDQENEKIINALSTGLGNLKMDKMGNYILDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP92</accession>
    <name>A0A1E5RP92_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GTP-binding protein RHO4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g975</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP92"/>
    <dbReference type="STRING" id="56408.A0A1E5RP92"/>
    <dbReference type="InParanoid" id="A0A1E5RP92"/>
    <dbReference type="OrthoDB" id="25896at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0488">Methylation</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="365"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="365"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="383"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ88513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="394" mass="44899" checksum="CA04C8984DA6E59A" modified="2017-01-18" version="1">MDHTTPMEGSTHADVENRSRSSTKHSYRPRAYTMPHSISSGQIEDYFADKQENKQHDIKQTLSKKFDQTWSTVDSDENIALQQQNIQNTEYGKDTELLFEPLSPIFSPAFQALSTSVFSRDTFSQKNHSTMLEQTLTLTNSSENVDMDYKLVGKRKKRNMKIVILGDSQVGKTTLLWTFTRKKYPVAQEIVVPRVFENYVVTVQLENIDANTPIKNGKPHDAESIDLVLWDVPGVLESQSGTDTNVSLSRPLAYNNVDLVIVCYSFDNLEKSFNSAKELWIPEARHFCVGAPIFLVGLKNDVIGSKVPKHMKQKIEKIEKIDKALMANKNNQFQNVVKHIKCSSKNLYNVDVLFHEAMQDIIKRNQTSTQKRTASHTTQPKNTSKHRGKQCILM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RPL7</accession>
    <name>A0A1E5RPL7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Histone-lysine N-methyltransferase, H3 lysine-4 specific</fullName>
            <ecNumber evidence="2 12">2.1.1.354</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g727</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalytic component of the COMPASS (Set1C) complex that specifically mono-, di- and trimethylates histone H3 to form H3K4me1/2/3. COMPASS recognizes ubiquitinated H2B on one face of the nucleosome which stimulates the methylation of H3 on the opposing face.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 12">
            <text>L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60260"/>
            <dbReference type="Rhea" id="RHEA-COMP:15537"/>
            <dbReference type="Rhea" id="RHEA-COMP:15547"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.354"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N(6),N(6)-dimethyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60272"/>
            <dbReference type="Rhea" id="RHEA-COMP:15537"/>
            <dbReference type="Rhea" id="RHEA-COMP:15540"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6)-methyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-methionine = N(6),N(6)-dimethyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60268"/>
            <dbReference type="Rhea" id="RHEA-COMP:15540"/>
            <dbReference type="Rhea" id="RHEA-COMP:15543"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the COMPASS (Set1C) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.354" evidence="2 12"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RPL7"/>
    <dbReference type="FunCoup" id="A0A1E5RPL7">
        <property type="interactions" value="146"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RPL7"/>
    <dbReference type="InParanoid" id="A0A1E5RPL7"/>
    <dbReference type="OrthoDB" id="3972033at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140999">
        <property type="term" value="F:histone H3K4 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024657">
        <property type="entry name" value="COMPASS_Set1_N-SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044570">
        <property type="entry name" value="Set1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017111">
        <property type="entry name" value="Set1_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048669">
        <property type="entry name" value="SET1_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45814">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45814:SF2">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11764">
        <property type="entry name" value="N-SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21569">
        <property type="entry name" value="SET1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037104">
        <property type="entry name" value="Histone_H3-K4_mtfrase_Set1_fun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01291">
        <property type="entry name" value="N-SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00508">
        <property type="entry name" value="PostSET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51572">
        <property type="entry name" value="SAM_MT43_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="12" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4 12 16" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 12 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="14">
        <location>
            <begin position="972"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="15">
        <location>
            <begin position="1098"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="671"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="897"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="57"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="107"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="676"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="686"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR037104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ88633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1114" mass="127401" checksum="5A154AD017BEBB1A" modified="2017-01-18" version="1">MSQSERSAANNTGNGTPVRAMPYFSRAQRTRPVETADWERSSYSPKEAETGNLRAQDFTSGHNGSHYSSVARTSEPMGSSYTGSSYSPLSAKPAARISSSYQPSRMLKREPDSRFTDKRSSYEYKSRYAVESNNNASFYKGNGSSRISKSRFDKSSPHITTVQQNTYVGKPFWETRPQGTVKYGSSDINSQWHYLDIQTNKLVNSQTFQTFHEHNPKIPKDGVVYDKSSNLLIKRTSSHKGHDPRERNNLLDSKKLTQKRLKVCLEVVYDKYSLGPPPISEIVVHPIQTELSKNMGTIIPDSVIENHFKNEFHLPISQFQSFSDPNSLVPLNVFVLKFSSPKNEDLNLPGKVAQKAVKKYENEPFVTNGMRFKVSLNNNALSSKIVNEIVQHNLKEMEKLLKPKVAQNEVRKENKDEKSKHFDFQEQELEKNHIDEVPVELRKNLNNRPVLFVPHSILQRYKLQSFDIKVSLRNLKFKLYTHVLGCFLSFDSLQNTMDYQNKGIDISSKRDRAPIRIKFLYIAPKRYQVQDNIFAGDSNLDGKRFSLYSKKAYTSKNDLLNDTVDLILSDLYSSIKKDIKTKIVGPAILNSLDKNKFPELLEQHEKELELKKAADLQRKKRTTITTSFDFLSLYSTNRKKPLKRTSGEAVARNGDLNSVKKVKRRRLKPLAHLLDEQGENSDEESETVKDEKEQSIDFKEETEFSATEELMEEEIEREVEKVSSERLQSPINETSKSAQFEDKFLPSVSEFPAPITPNLSISTLPILDLYTATEDSEDLTFLKKVMLERSGLHKNLAQKPMTEPVANYLSTKKRRYFANREKHQILLKLNYGLEMDCSVLNKNASFKASGYKKLTSYHKDTFIPRRRKAGASKTVQTHNEDMYEDDMLVVASSHLRATSPDNELPKENEQKAASIDNQENGEIANLKQEKGHSSRVNRVLNRRFQNEMEHHKQMLGKETDLLSLNQLSKRRKPVTFARSSIHNWGLYALEPIAAREMVIEYVGEIIRQPVAEMREKTYIKSGIGSSYLFRIDENTVIDATKKGGIARFINHCCDPSCTAKIIKVGGTKRIVLYALRDIAANEELTYDYKFERETDDSERIPCLCGAANCKGFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV40</accession>
    <name>A0A1E5RV40_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Probable metalloprotease ARX1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Associated with ribosomal export complex protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g403</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Probable metalloprotease involved in proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit. Accompanies the pre-60S particles to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RV40">
        <property type="interactions" value="411"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV40"/>
    <dbReference type="InParanoid" id="A0A1E5RV40"/>
    <dbReference type="OrthoDB" id="5876363at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047113">
        <property type="entry name" value="PA2G4/ARX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10804:SF102">
        <property type="entry name" value="METALLOPROTEASE ARX1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10804">
        <property type="entry name" value="PROTEASE FAMILY M24 METHIONYL AMINOPEPTIDASE, AMINOPEPTIDASE P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 13" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ90801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="604" mass="66859" checksum="5277F8B2993B3653" modified="2017-01-18" version="1">MALAISNEDTNILLKDKNVLNEPVLDKYRIAGQITQTAITYLKNLINDSYHYKTXDPPLSISQLCFLTDLFVSQTLETFYKNKVNERGISHPCSIDIDEFNNGWSPELDDFPLLTNKNKSSDYTNGMHSAINNVLKDGDLIKITLGCHIDGYTSQVTHSMCIYPTIENNTKPQGPLLGGKADAIAASHIATETIVSLLACSLHPEKLPQAFHSVSQSQINGTLMRSVVDTIAQSYNCCVVPGSKIRRIRRFLAGQNEGVVAERDFKGVVWYESNQEADLLNKSKSFNENSNNKEESALVASTKSNNSHNFLEDSAIAKDDFQVLPGEVYLIDLKFAPLTGEAGNKLGLVTLETLNEFSGSTTTNKNITIRNSQFVRDYTQTHILKLKTSRAVLNKLDKQGVYPVKLAHLSDDFLTCSPQDVIANNLQNINKDLKSIRMGMNEITNNYLATSKPVQIVKFIPWDLILNCANPSGNKGVDAINPTLPGFEVPLPKLNISSMKLKSLLKNRDCVALPMARESCVVAVSELEDGVLRLTGGSKTCAPIWCHSQYQLDPNNNITQGIFQLTELSKDERFGLKIREVQPMKKVLLKSKQLAGADADSMMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV44</accession>
    <name>A0A1E5RV44_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 15">DNA replication licensing factor MCM4</fullName>
            <ecNumber evidence="3 15">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g503</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the MCM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3 15"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RV44">
        <property type="interactions" value="1029"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV44"/>
    <dbReference type="InParanoid" id="A0A1E5RV44"/>
    <dbReference type="OrthoDB" id="10251574at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097373">
        <property type="term" value="C:MCM core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006267">
        <property type="term" value="P:pre-replicative complex assembly involved in nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17755">
        <property type="entry name" value="MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000003">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1640.10:FF:000011">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000217">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008047">
        <property type="entry name" value="MCM_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF66">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21128">
        <property type="entry name" value="WHD_MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01660">
        <property type="entry name" value="MCMPROTEIN4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 15" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="7 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="17">
        <location>
            <begin position="560"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="24"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="87"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="102"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="131"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="194"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ90820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="980" mass="108941" checksum="0E97215D0905FAB7" modified="2017-01-18" version="1">MSEPSSPIGGGNPPENSSPFPSSDIPSQTQPIANSSSSSTRRNNAQMSSPMQGIESSPRAFDSPLIRRGTQERDTYSDASSQSQRRPMASASSPMAFPSSSLGSNLHSQRNIPASSSSMLGSSRATRNRTLDSQNSRAXPSSSFYSGSERVRNMGRRNDISSSDVSAHRRMVDMSDLSSSPRPLHSNALPSNGLASSSSVIPSDDLSAPNEPVRVIWGTNVSIQECVNKFREFLMSFKFKYRKELDNRGDSVDFEGDEELFYIAKMKKMIQARTTNLNLDTRNLLAYKNSEKLYHQLLNYPQEVISIMDQTLKDCIVQLCVDENMSDSTLDDIESRFFKVRPYNIDNKKGMRELNPGDIDKLVSVKGLVLRSTPIIPDMKVAFFKCSICNHTTVVEIDRGVIQEPPRCPSPSCSQPNSLVLVHNRCSFSDKQVIKLQETPDLVPDGQTPHSVSLCVYDELVDSCRAGDRVEVSGIFRSIPIRANPKQRSLRALYKTYLDVVHIQKVDNTRLDLDTSTVEQEVLQHEVQADEAGGSGVQELRKLTDNDIRKIEEVSKRFDVYDLLARSIAPSIYELDDVKKGILLQLFGGANKVFKKGGKYRGDINVLLCGDPSTSKSQLLQYVHKIAPRGIYTSGKGSSAVGLTAYITRDVETKQLVLESGALVLSDGGVCCIDEFDKMSDATRSVLHEVMEQQTISVAKAGIITTLNARTSILASANPINSRYDPDLPVTDNIDLPPPLLSRFDLVYLILDKVDENLDRELATHLTNLYLEDRPQNGSSASQDILPVEFLTMYINYAKEHCKPVITELAKDELVRNYVEMRKLGDDSRSEEKRITATTRQLESMIRLSEAHAKMRLSQEVGLEDVHESVRLIKSALKQYATDPKTGKIDMSMVQTGKSAVQLQIQEEIARNVLSMLNNSTEDQIAVNELIKQIKESGQQNVENSDVVEALSRLQQEDKIVIKPVRGTRVVSLNTMTTHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVA1</accession>
    <name>A0A1E5RVA1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein DSS4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g505</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVA1">
        <property type="interactions" value="58"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVA1"/>
    <dbReference type="InParanoid" id="A0A1E5RVA1"/>
    <dbReference type="OrthoDB" id="30840at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006892">
        <property type="term" value="P:post-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.10">
        <property type="entry name" value="Metal Binding Protein, Guanine Nucleotide Exchange Factor, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007515">
        <property type="entry name" value="Mss4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011323">
        <property type="entry name" value="Mss4/transl-control_tumour"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13276">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR MSS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13276:SF0">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR MSS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04421">
        <property type="entry name" value="Mss4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51796">
        <property type="entry name" value="MSS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ90814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="163" mass="18340" checksum="ECAC9DE628E5701B" modified="2017-01-18" version="1">MAQLSNDSRMRATCPFEKCASKIIAYQDDQVIELPSYFSELYKLMXSSESVNDATRILPTKFLVTQDIWDFDNIGVSKSLDTLDLEHKVQVLAEEGNAQYSFKNIVIPYKNTNYKIVKALKYLICADCDKGPLGVXLEVFPVGTLDNASHIQLNLLNVDVCKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVF2</accession>
    <name>A0A1E5RVF2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g502</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVF2"/>
    <dbReference type="InParanoid" id="A0A1E5RVF2"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ90819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="272" mass="30581" checksum="CB02EE347B55F7C9" modified="2017-01-18" version="1">MLVDLATISSAPSDNEKPTEGMQVIYDQLKKFCASGDENRHILVFDDVFNVAQSFFLPINDEFEKRLSGAMIALHRFISNGYFSSELNTNLLGIQKSKEVENLTAFEAISTQAPKNGERKIAFAKNNKQLTFEPDDIIEKRSKSLQNSNKEIKMSSILRVKNNPYAESEKLKSLRNDNVSVFDMLAEIISTDKSTKTRDEVAKGNAIRTAQRREQLANYFFTELSSLVRIQNFEVVLSVVGSLCATQKNFGINLNCSEVRGQIKDAEMGDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVL3</accession>
    <name>A0A1E5RVL3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcriptional activator HAP3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g482</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NFYB/HAP3 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVL3"/>
    <dbReference type="FunCoup" id="A0A1E5RVL3">
        <property type="interactions" value="737"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVL3"/>
    <dbReference type="InParanoid" id="A0A1E5RVL3"/>
    <dbReference type="OrthoDB" id="386949at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22907">
        <property type="entry name" value="HFD_NFYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027113">
        <property type="entry name" value="Transc_fact_NFYB/HAP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003956">
        <property type="entry name" value="Transcrpt_fac_NFYB/HAP3_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064">
        <property type="entry name" value="CCAAT-BINDING TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064:SF9">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00615">
        <property type="entry name" value="CCAATSUBUNTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00685">
        <property type="entry name" value="NFYB_HAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="6">
        <location>
            <begin position="33"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="127"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="132"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ90971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="200" mass="22758" checksum="AF023A5E768B62E4" modified="2017-01-18" version="1">MSNPDDQTEPTEDHHVSESYSKMLRSLTEQDRLLPINNVSKLMKSVLPPSAKVSREAKECVQECVSELISFVTSEASERCLVDNRKTVNGEDILKALEDLGFENYSEVLKIYLAKYREYQIIKHNSSPSLENGRKQHMSDQEQHASQQPFGAYSVSKEATMPAQPRSQFAETNDDVEYGTKEEQMTHANREVDSDNSEHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVQ9</accession>
    <name>A0A1E5RVQ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acetyl-coenzyme A synthetase</fullName>
            <ecNumber evidence="6">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g288</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>acetate + ATP + CoA = acetyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23176"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVQ9"/>
    <dbReference type="FunCoup" id="A0A1E5RVQ9">
        <property type="interactions" value="756"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVQ9"/>
    <dbReference type="InParanoid" id="A0A1E5RVQ9"/>
    <dbReference type="OrthoDB" id="1706066at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019427">
        <property type="term" value="P:acetyl-CoA biosynthetic process from acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05966">
        <property type="entry name" value="ACS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000001">
        <property type="entry name" value="Acetyl-coenzyme A synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011904">
        <property type="entry name" value="Ac_CoA_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02188">
        <property type="entry name" value="Ac_CoA_lig_AcsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001208">
        <property type="entry name" value="PRK00174.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF245">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="9">
        <location>
            <begin position="40"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="7">
        <location>
            <begin position="98"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="8">
        <location>
            <begin position="547"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ91030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="677" mass="75155" checksum="4027064BDB89EF92" modified="2017-01-18" version="1">MTMDHLIVHEAHNVAMRDTPQHFYDKQPSRGSYIKNKDQYTEWYNQSINNPEQFFGNMAREYLHWDKDFTKTLTGSLEKGDVAWFLNGELNACYNCVDRHAFANPDKIALIYEADDEKDNYKITFNELLTRICKVAGVLQKWGVKKGDTVGIYLPMTPEAIIAMLAVARLGAIHSVIFAGFSAGSLKDRIVDAGCKVVITCDEGKRGGKTVNIKRIVDEGLNGVQSVSHILVFQRTGNPQVPMTAGRDYYWDAECAKQRSYIPCVPINAEDPLFLLYTSGSTGSPKGVVHTAGGYLLGAALTTRFVFDLHPEDVFFTAGDVGWITGHTYALYGPLSLGTSSIIFESTPAYPDYGRYWRIIERYQATHFYVAPTAMRLIKKVGEAEISKYNISSLRVLGSVGEPIAPDLWEWYNEKIGNENCVICDTMWQTESGSHLIAPLAGAFPTKPGSATLPFFGINAAIIDPVNGQELTGNDVEGVLAIKSPWPSMARSVWNDHQRYMETYLKPYPGFYFTGDNAGRDHDGYYWIRGRADDVVNVSGHRLSTSEIEVALSEHPAVSECAAVGIADDLTGQAIIAFLALKNEEMEHPANPEDLQLLRRELIMKVRGSIGPFASPKSVIIVEDLPRTRSGKIMRRVLRKISSNEAEELGDLSTLANPEIVPKLLSAVDAQFLSLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVR7</accession>
    <name>A0A1E5RVR7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g379</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVR7"/>
    <dbReference type="InParanoid" id="A0A1E5RVR7"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="70" mass="7875" checksum="08488839A12F6A2B" modified="2017-01-18" version="1">MLSSRVLRFATSRSVMSASTKFSKDQVYKSSPTLWLGKEGKRLTKNIGIWAGFMAVCICWPYSIKFLAGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVX7</accession>
    <name>A0A1E5RVX7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g567</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RVX7"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="318" mass="34888" checksum="F3FA066BAD088475" modified="2017-01-18" version="1">MKDAAKIKAAKMKDAAKVCASKMKDEAKVEADKIKDXXKXXADKMKXDGKVXADKXKDAAKIKAVKMKADGKVRADKMKDAAKIKAAKMKDDGKVCADKMKDAAKIKAAKMKDDGKVCADKMKDAAKIKAVKMKDAAKIKAVKMKDAAKVCADKMKDKAKIKAAELRVNAKVCAAKMKDAAKVCADKMKDXAKXEAAELRVNAKVCXAKIKKLIAHFNFVAYTHENEDENDDDNKNDNKNDEEYLDDDEEHEIYKXIEARRYSISRXNDYXGSXKTLAHTSNITSLGGFNTTSIPNFAPCSIGHTAEQEDGEGLNTIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVX9</accession>
    <name>A0A1E5RVX9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Vacuolar membrane protein PEP3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g295</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endomembrane system</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVX9">
        <property type="interactions" value="894"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVX9"/>
    <dbReference type="InParanoid" id="A0A1E5RVX9"/>
    <dbReference type="OrthoDB" id="1845386at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048284">
        <property type="term" value="P:organelle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16462">
        <property type="entry name" value="RING-H2_Pep3p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007810">
        <property type="entry name" value="Pep3/Vps18_beta-prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058919">
        <property type="entry name" value="Pep3/Vps18_RING_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323:SF26">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 18 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05131">
        <property type="entry name" value="Pep3_Vps18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26148">
        <property type="entry name" value="VPS18_RING_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Pep3/Vps18 beta-propeller" evidence="6">
        <location>
            <begin position="1"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Pep3/Vps18 RING C-terminal" evidence="7">
        <location>
            <begin position="849"/>
            <end position="928"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF26148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ91016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="947" mass="108598" checksum="6BFF67817EB185AC" modified="2017-01-18" version="1">MKVTIEKVQFEQSIQGQICCLEVTNNIMHFVMFQSTVQGNSLKMFVIDLDHPEKVQIFDIPHLKENEIIAKTWGISSPKTVQNGIMLMSDGGKFYHYSNDTGKITFLKKLSKKQHFTPNSFASIISKKEEDKEEDKECILIGTRQSKLYRYNLKTEKMSKLHEWSRDFVEGISYADSSLWVIVGSAVSVFSNLNSVKDLKTNTQPSIVEVYEKCSSKIQHLQHKRFTENPNTNSFGWITKNGVVFGSLFESPSQAKVLLNVELPLYNDSVITDIFLSDYHIMLLTQEKQLLIVNQLNNKVVFEEKINLPSDEEIQGFKVDYIKNTFWLYSKSSVFEIVVKNEYTNVWKTLLEMNEFEKILSIKDFPQLDKEFVYQKYGDYLLSTNQVLKASEMYSHSNSDISLTTLKLLENQDISALQNFFXQRLAHSNLTKMQQKLYTSLILWNYVQMQVEDRVVREFLIKYVNKIDKTLAYDVLKYQKTASNLLFYANLIKDYKFVLDHWISLENWYEASKILLLLNQEQYVYQYATVLLGNDPETTVQMWIKLGSTVQIYPEKLIKPILCYYSSCYLRAIGTENHALNYLTWCVANEQLIANDYSRNTNGLKHTSKIITPLVHSTILYMMITDKKNTAEFKIIEYIKAHGGKFDSNFILNLSLKFDRIETAINLYSDMNLLEEAVNLAIKHKMLETAKRIASFSKGSVYSADATVGNGAGYDFSSSATTNFTPGRAAILLDDSEVSSEIAVRKPLWIKIAKVLLLDPMARKNGKDTKQTITDIILESNGVLTIKDLIPLFDSMTTIANVKDELIKSLEQHADSMQQISHEITNLVNIKKNILGQMELLDEAYAQIKPGAVCDGCDTFLQTKKFYCFPCGHNFHINCLISAILKGGDFMLSGKIQDLQKKRKANWVKELDQLIAAKCPFCSEISINNIDEPLLHQSSFDDNFLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZ71</accession>
    <name>A0A1E5RZ71_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Flavoprotein-like protein YCP4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g135</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WrbA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZ71">
        <property type="interactions" value="200"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZ71"/>
    <dbReference type="InParanoid" id="A0A1E5RZ71"/>
    <dbReference type="OrthoDB" id="504689at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032126">
        <property type="term" value="C:eisosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003955">
        <property type="term" value="F:NAD(P)H dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160020">
        <property type="term" value="P:positive regulation of ferroptosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000001">
        <property type="entry name" value="NAD(P)H dehydrogenase (Quinone) FQR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010089">
        <property type="entry name" value="Flavoprotein_WrbA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01755">
        <property type="entry name" value="flav_wrbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002999">
        <property type="entry name" value="PRK03767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546">
        <property type="entry name" value="FLAVODOXIN-RELATED PROTEIN WRBA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546:SF23">
        <property type="entry name" value="FLAVOPROTEIN-LIKE PROTEIN YCP4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="3">
        <location>
            <begin position="4"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="202"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="236"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="246"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ92300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="260" mass="27538" checksum="2D9797278589B580" modified="2017-01-18" version="1">MVKIAIVVYSTYGHVLTLSKQVLKGVQEAGGDADLFQVPETLSKETLALIGAPGQDTXEIPLATPEVLAQYDAFLFGIPTRFGTVSSQWAAFWDQTGAQWSSGAFAGKPCGLFVSTGSPGGGQESTIKNSLSYLVHHGMIYVPLGYANVFGELSNTEEIHGGSSWGAGTLAGVDGSRQPTALELXVAFTQGKTFYKXASKLSPNVKAGNPKSPSKRVESTEKTVLATAEKPSIQQKAAPKKTNTTKPKEESKDNKCCVIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZ81</accession>
    <name>A0A1E5RZ81_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">V-type proton ATPase subunit e</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g120</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the V-ATPase e1/e2 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZ81"/>
    <dbReference type="FunCoup" id="A0A1E5RZ81">
        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZ81"/>
    <dbReference type="InParanoid" id="A0A1E5RZ81"/>
    <dbReference type="OrthoDB" id="1508846at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008389">
        <property type="entry name" value="ATPase_V0-cplx_e1/e2_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12263:SF0">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12263">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05493">
        <property type="entry name" value="ATP_synt_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ92282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="76" mass="8667" checksum="3F6D13D6ADAED155" modified="2017-01-18" version="1">MASSFYTVVIVFLVVALINTIAWFLAPKKGGNTTVFRSTFILALSMMYLMWAITYLAQLHPLVVPRRSDLRPEFVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZR9</accession>
    <name>A0A1E5RZR9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cell division control protein 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g132</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZR9">
        <property type="interactions" value="256"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZR9"/>
    <dbReference type="InParanoid" id="A0A1E5RZR9"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000144">
        <property type="term" value="C:cellular bud neck septin ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031105">
        <property type="term" value="C:septin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000921">
        <property type="term" value="P:septin ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043934">
        <property type="term" value="P:sporulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000260">
        <property type="entry name" value="Cell division control 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 9" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="8">
        <location>
            <begin position="42"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00069702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ92299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="335" mass="38140" checksum="86DD9578CD1C0829" modified="2017-01-18" version="1">MSSTTVPTHVPAGQAATNVIEPKGYVGFDTITSQIEHRLLKKGFQFNIMVVGXSGLGKSTLVNTLFSADLIDRENPSFDHLPKTTEIKLYSRTLVEDKVQLKINLIDTPGFGDQINNNKAWEPIIKYIKEQYSQYLRKELTAQRERRVEDTRVHALLYFIEPHATKGLTELDIAVLKKLTDIVNVVPVIAKADSLTLSERNMFRSRIQQEFSALGLKLYPFDDHYDDLTEDELELNSKIRSIIPFAIVGSNQTYTNKDGKLVRGRKTRAGDVIEVENSSHCEFVYLREFLIRTHLQDLIESTALMHYETFRAKQLIALKENASNNRQSQSGALQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZW5</accession>
    <name>A0A1E5RZW5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Putative metalloreductase AIM14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g70</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZW5">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RZW5"/>
    <dbReference type="OrthoDB" id="17725at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000293">
        <property type="term" value="F:ferric-chelate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033215">
        <property type="term" value="P:reductive iron assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050369">
        <property type="entry name" value="RBOH/FRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972:SF198">
        <property type="entry name" value="METALLOREDUCTASE AIM14-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972">
        <property type="entry name" value="NADPH OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00463">
        <property type="entry name" value="AIM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="106"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="176"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="395"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Ferric oxidoreductase" evidence="13">
        <location>
            <begin position="107"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Ferric reductase NAD binding" evidence="14">
        <location>
            <begin position="394"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ92491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="623" mass="71324" checksum="069BDBE18628B242" modified="2017-01-18" version="1">MNALEKRHGGTHHANDHYGTFLLYFQIAYIAVVLVLVKKFASLKKISGFGNSRSSNSKVQNLLNRLYSLDPLVHMLMILVIIFIPFPTSKFGEYIKSHYTVYFKKFGKISYCMLLLNLYLVLPRSNTFMIMSYLDSIRFHIWLSRFLLLCAILHSVMFLIRWALQGVLIQKLCKTKYFIGLIIFVWTCVLFGITSIFPGFRRKNYRLFYLIHQITMFLYVVLTIFHADPQVLVPFGILNCVSLLIFVTNRFINYEIVKIVEKSTVNGSQLGYYEIASATQNDQQVLGKQYILPGSHLRLHSYSPYHWKHWFYPSHPFTIVGHSTSNRAKHANSQSVSLVVKQSSANLQNMLHQNKTFYTCGVYPPSKDVQELVNSFEPSTSNENEINRPISRKKLVFVVGGSGFSFAFALYLHVLKATISNTSAIPFVKFNWIVRSAKDLHVLEHFNELDFLEKNDKEASLGNGKNYPDMEVFITNNTVTELGAASPERSLETTPSTMINTSDFDDXENEGFEMGDASDFNASVNIHSLPKYLALQTNDGNLVTVKVNYHVNKRFNVPLDLTENLDFIAPDEGAFXNGLEFPDSEQPTEKWLCCCGPESLIEDGQNFSKRHSDIIYVKEEYQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S019</accession>
    <name>A0A1E5S019_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Mannan polymerase II complex ANP1 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g189</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ANP1/MMN9/VAN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5S019">
        <property type="interactions" value="197"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S019"/>
    <dbReference type="InParanoid" id="A0A1E5S019"/>
    <dbReference type="OrthoDB" id="204164at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000017">
        <property type="entry name" value="Mannan polymerase II complex ANP1 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052086">
        <property type="entry name" value="Mannan_Polymerase_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083">
        <property type="entry name" value="MANNAN POLYMERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083:SF2">
        <property type="entry name" value="MANNAN POLYMERASE II COMPLEX ANP1 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03452">
        <property type="entry name" value="Anp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="441"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="445"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="470"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="499"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ92474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="528" mass="61033" checksum="9098832DB6A2A8ED" modified="2017-01-18" version="1">MPYKHSKKRKTALVAILSSLFTLIFLYEFVTKVYKIPGLSSVLLSSTGSFSNKFGFTSFNDKFQIYEPDFSKTPQVEYYDLQKYKGNKDGWQTQDRIVFCVPLRDAAEHLPRFFKILSQMTYPHNLIDLTFLVSDSKDDTMGALKNELEFLQSNNDKTKRYGNIEIYEKDFGTIIGQSFSDRHGFAAQGPRRKLMGRARNWLGSVALKPYHSWVYWRDVDVEVVPDTIMEDLMHHDKDVIVPNVWRPLPDWLGNIQPYDLNSWKESGGGIQLAETLDEDAVIVEGYPEYATWRPHLAYMRDPSGDPEVEMELDGIGGVSILTKAKVFRTGSHFPAFSFEKHAETEAFGKLSRRMGYNVVGLPNYVVWHIYEPSTDDLKHMKWMADEEERILEENKIKDFYDKIWEIGFEDVRSSWEKDIHNILKNSDSTFQRKIDVDWSENEEQDYVKKEEPKRESEGEGEGENXGXGEGEGEGDNTEEDITALEEEAKKKAESLNLDKSSKSGKKTEKKKEQEEEDDSVWDMLSNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S020</accession>
    <name>A0A1E5S020_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g240</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5S020"/>
    <dbReference type="InParanoid" id="A0A1E5S020"/>
    <dbReference type="OrthoDB" id="3972692at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051432">
        <property type="entry name" value="KCNMA1_auxiliary"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46473">
        <property type="entry name" value="GH08155P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46473:SF10">
        <property type="entry name" value="LD45603P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="71"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="116"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="255"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="438"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="71"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="91"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="280"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="439"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="452"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ92436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="915" mass="101884" checksum="E08576724CA3974E" modified="2017-01-18" version="1">MNGRNHNLGPRVTGKMDVGSSTVHDDANSDNEDSLDAGSPLKNLSRSYQLGSIGHVNSKYIALDIDNMPSLQEENNNGYPRQSQDSEQKSVVESTGNNNYSSSFAEYDTVINSPEKKSVSAQEEAHYADGSPHAPALSKSENGGAQWKNFFQPRNRPQLVNGGNMLGSYKHNNEHGAATIENRMNNGGDDQYYSNLLPDVSSHIIKHDASEDEEDSKLGQSNSFNRFTETHDENDKSIFHYEFAEKTNNYTSSFYNEKEINGPDSDISKAQMQSQKNNEEGEQEQEEQEQAGIEEPNNTFLKRKKGISAFNVPQSIPEYHMTNTSDNSNVSGDPAPDARNVFANLFSSNIKPIDNTNNNVQRHYVNADAAPHPTASGLSNATTAIASASSNPAFQQNVGQMKNKGIAPSGHKKALSTTTPQDVGMFYDEKTGVWANPHNSSSMGSANASANTMGDPSLESSRNTVNISGIKARNLNKNGDFKQPSLKTPSTAGVAYNDSLDYEDTSVLPDPIISPLEENLEHDHDSYLNEPETKYKALLMERLIQLAGTTLGSIYDLDISSGMLGLEVQDYFCSLFEMLPNLLELKCSNNGLHEVCLPKFTNLESLNLSENKLKEVNTLLNGVHLHLRSLDLTHNLLTQFETSSAKCAFFKLQNLNLSQNSFSGHFELNLFEDFPNLQTLDVSDNQGLTHISLKRGTKPLRLLLNNNSQLQKVTLVDDELPTDTLIFQTLEMENCLPTLIAYMNTKYQDNIAHMVISSVTPTSLNASWKFARSLESLIVKKYWLQDDWYRLNEGLEQLLITEIISDINCSFPKAGGSLPGNNLKKLHALSIANTNLSFPLSFKFDFLFENLQIKNLHELNVKENVEIKSKIYTQFDIYEQDRVEALYRHGTQKKFGGSLTMLDGRAGFFDWDMQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S042</accession>
    <name>A0A1E5S042_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein CWH43</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g79</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5S042">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S042"/>
    <dbReference type="InParanoid" id="A0A1E5S042"/>
    <dbReference type="OrthoDB" id="68581at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000031">
        <property type="entry name" value="Calcofluor white hypersensitive protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019402">
        <property type="entry name" value="CWH43_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057315">
        <property type="entry name" value="Exo_endo_phos_PGAP2IP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051916">
        <property type="entry name" value="GPI-anchor_lipid_remodeler"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053912">
        <property type="entry name" value="PGAP2IP_TM_1nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053911">
        <property type="entry name" value="PGAP2IP_TM_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14859">
        <property type="entry name" value="CALCOFLUOR WHITE HYPERSENSITIVE PROTEIN PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14859:SF1">
        <property type="entry name" value="PGAP2-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23022">
        <property type="entry name" value="6TM_1st_PGAP2IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23021">
        <property type="entry name" value="6TM_2nd_PGAP2IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23226">
        <property type="entry name" value="Exo_endo_phos_PGAP2IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10277">
        <property type="entry name" value="Frag1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="101"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="129"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="158"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="317"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="346"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="371"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="396"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="427"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="466"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="506"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="551"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="577"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="601"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="631"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="667"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="699"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="domain" description="CWH43-like N-terminal" evidence="3">
        <location>
            <begin position="14"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="PGAP2IP first transmembrane" evidence="5">
        <location>
            <begin position="323"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="PGAP2IP second transmembrane" evidence="4">
        <location>
            <begin position="510"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="PGAP2IP C-terminal nuclease-like" evidence="6">
        <location>
            <begin position="733"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ92494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1022" mass="116588" checksum="358095E31C68B62D" modified="2017-01-18" version="1">MTSQEHGAVKINGSSIILMHTVCASMAFLAALAIGYHLHFIKIVTNAHYSYPDEWWPSVSATIGDRYPERSVFQILIALTSFPRFCLLLTHFFLTQSKAQFVVGVLRTVTCGGWVYITSTDDHDSHDVFMISYIILTLPWNVLCINNSKPETKKYKKLACFSFFGTLVPLIYWFIQHEVKVRPGAYSIYAYFEWSLIMQDILFDALSYKDFCAIDLIFQTSGIKLVEHHDESMKADKEIDEKTTSQNADTIHLVDEDDEDDEESDPEYVDSDIADENDETEVLNIVEIEKDAKALLQEEQNLVSVPPFMDTTAHFALVVYQSFMLWTSLTALLCIIWHFPLWYMGISGYEAGILSVLLSALMYLPYSPQIMHQYGSLLITFTSLGSYLVTVPEKRLITIGFGVGLACMNLVYNFQYALKSKEAVRKFSIAWCLGLALSVILKMGNYSNNPLWPIMFEENGGWNKTGIFLSTVVSFISPTLIAPPFASNCNTFLNTEKKHSLCVKKVFDIVVVAFGFGSLIFATHQLLTDASTIIYWSWEGYKIDEKPLLQWPWSFVTCAFMVLSSVLLCRNISQNKALIVTLLVFSTYVLSNPAFTSFSNYFFGGLPYIVGLILAYSYYFQQLFRLQSIAAFTLAFFVYDILILAHVWTVAYAFVPFGWVLRERIEYVLSASTGLIVLGIICTSSKALPFLVDSNLNKLCLRVSVFLLVLASYFTYTMQPNKTDPEPYHPNKNLCTAGIWTIHFGLDNDMWASEQRMIDLLQDLEVDVVGLLETDTQRITMGNRDLTNKMAHDLNMYADYGPGPNKHTWGCVLLSKFPILNSTHHLLPSPVGELAPAIHATLDMYGEPVDVFVFHSGQEEDEEDRRLQTQELTRLMGSVPEGRPTLLLSYLVTDPHEGNYNTYVSEQSGMHDIEPADDDRWCEYILYKNLKRTGYARVHRGTITDTELQVGKFQILSQNELKEYGDELIYQSHKVESLHDDEFKFPDMFLEDGERGHYYHVFDKPYYYDFDLSXFXKFRGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0E3</accession>
    <name>A0A1E5R0E3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein JLP2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g4420</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0E3"/>
    <dbReference type="FunCoup" id="A0A1E5R0E3">
        <property type="interactions" value="547"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0E3"/>
    <dbReference type="InParanoid" id="A0A1E5R0E3"/>
    <dbReference type="OrthoDB" id="200398at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039730">
        <property type="entry name" value="Jlp2/Ccd25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13049:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13049">
        <property type="entry name" value="DUF814-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NFACT RNA-binding" evidence="3">
        <location>
            <begin position="11"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="205"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="223"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="235" mass="27421" checksum="1F0A6547740A9649" modified="2017-01-18" version="1">MYAYISHPEDTERPYWIFMGRDKFENDLLIKLGYKEQNFYWFHADKYSSGHVYLKLHRDESGIADVPKVVIQDCLQLCKSESIQGNKLPECKIIYTPWTNLRKSGHMSPGEVSFKSSSRCGKLMCYQRDSSVLSRLKKTKVDVVDLNKAEAPFMSQQEKIDNGYLFLENLLRTAKKSKDGEYLVTYVEQNRAALIELEALMKQRNKQAKKEQAKSKKQKLKEEEEAWLNDDLSDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0I8</accession>
    <name>A0A1E5R0I8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Carboxypeptidase</fullName>
            <ecNumber evidence="7">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4520</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0I8"/>
    <dbReference type="InParanoid" id="A0A1E5R0I8"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF113">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE CTSA-4.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 8" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009028090" description="Carboxypeptidase" evidence="7">
        <location>
            <begin position="21"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="493" mass="54845" checksum="1AD656916DDB6289" modified="2017-01-18" version="1">MLSLQHLIAASLLFLSVSFAGPVFPDAFFEEKHSFKLPQNRYQSVKKDISNSKDLETFALESHPNHKLRTKVVDPSKLGVDDVFQVSGYLDVEDEKKHFFFWYFDSRSPMNETDDLIMWLNGGPGCSSFTGLFGELGPASITEDLEVVKNAYSWNSNASMIFLEQPVGVGYSYFDEDADSVTGSNAAAKDVYAFLDLFFSKFPHLISKKFHISGESYAGHYLPAIANEIAIVHKEDAKFKLSSVLIGNGITDPIKQHRSYYDMACNPALSGVPQLVSDSDCHKMQANIPRCEALLTACYASELDFACVLANTYCDAVGLEAFSKTGLNVYDVRGPCETDNGNCYLVSDYIDAWANQPSVQNALGSDIEKYTGCSNDVYGRFAFSGDGAKPFQQYVAQILDQGIPVLIYAGDKDFICNWLGNMYWTDALDWKYQEDFAAVNLQPWYVDGAYAGQKKSYGGLTFLRVYDAGHMVPMNQPENSLAFLNSWILXGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0N5</accession>
    <name>A0A1E5R0N5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Trafficking protein particle complex subunit 20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g4540</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0N5">
        <property type="interactions" value="335"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0N5"/>
    <dbReference type="InParanoid" id="A0A1E5R0N5"/>
    <dbReference type="OrthoDB" id="10252102at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14825">
        <property type="entry name" value="TRAPPC2_sedlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006722">
        <property type="entry name" value="Sedlin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12403">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04628">
        <property type="entry name" value="Sedlin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ80456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="206" mass="22488" checksum="4DA262E665589C0D" modified="2017-01-18" version="1">MQGAEYFAIIANDKDYPLYEASFTESSTNASAELFPFIANSALDIVEDIQWKTSHDELQQQLQLQQQLMLQQQQGTAASAASAAATSSSTSTMASFFGGGKSSSQTASSGAFGAGSSQVSLGSLYLGCIDHYYQTPISAYITFGNVKFILIHTSNXSVNNNNTRLFYQLVHELYIKTIMNPFYTNGDIIASPMFDAKVRSFAKKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0P3</accession>
    <name>A0A1E5R0P3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitochondrial adapter protein MCP1 transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4396</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0P3"/>
    <dbReference type="FunCoup" id="A0A1E5R0P3">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R0P3"/>
    <dbReference type="OrthoDB" id="10259513at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039960">
        <property type="entry name" value="MCP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38409">
        <property type="entry name" value="MDM10-COMPLEMENTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38409:SF1">
        <property type="entry name" value="MITOCHONDRIAL ADAPTER PROTEIN MCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="283" mass="32755" checksum="260BE76658D1E681" modified="2017-01-18" version="1">MPPHLKAADPEPFTASEHEYASGTSSNDSPDRKTHSTRRWLGIPIPKNATIFRQLNYVHKYSTVPYLIFIPFHLFNVLALPTALSFLYSKEEKYQLLNEYYFLLKELNGLIKFTGMIVGGLSLHIVSGIILNQWKNYRKNIFIKRKEEEEEEEEKPVRALQNGVMDRKSWFRWKNWNYNAITGRVAVPLIMYHLYLVKLGGIEKLGSKAHETIMEAISDFIRHNFWEGTVPFVTLVSVVLYHTSFGVSRYFNHNRLNPSKYQSRGWYVTAGAIVAAMAGLMFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0S8</accession>
    <name>A0A1E5R0S8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein RCR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4499</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0S8">
        <property type="interactions" value="43"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R0S8"/>
    <dbReference type="OrthoDB" id="4088875at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020999">
        <property type="entry name" value="Chitin_synth_reg_RCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28187">
        <property type="entry name" value="PROTEIN RCR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28187:SF1">
        <property type="entry name" value="PROTEIN RCR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12273">
        <property type="entry name" value="RCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="186"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="246"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="280" mass="30250" checksum="7672A044DF021A82" modified="2017-01-18" version="1">MYVPEXLFDKRLYLYDGYGGSGSAPWTRYLYIIFIXVGFFVFLMAANAFRIRRGGRPIRGTGWAAPPSYFQSQLRENGEAHRRRQQNEGPPVPLYTEDVNNMDAGYFDDNGIFHPNPNLHPPEVPAGGFPPPKEGDPDSNIHPPQSAPVHDERTHGTVLRDMHNNTIPVATSGTATPDVTGMNRVGGSAAGSAAGSAAGPSTTRTTATTATTQVHQNDTHTNDNYMNDSSTNLVKTGGGVGEPLVTTLNTSSGTTTSEEEIDKESIQFKRPSHPPPGFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0U4</accession>
    <name>A0A1E5R0U4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">asparagine synthase (glutamine-hydrolyzing)</fullName>
            <ecNumber evidence="2">6.3.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g4583</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-aspartate + L-glutamine + ATP + H2O = L-asparagine + L-glutamate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12228"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.4" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0U4"/>
    <dbReference type="FunCoup" id="A0A1E5R0U4">
        <property type="interactions" value="592"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0U4"/>
    <dbReference type="InParanoid" id="A0A1E5R0U4"/>
    <dbReference type="OrthoDB" id="409189at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00712">
        <property type="entry name" value="AsnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000031">
        <property type="entry name" value="Asparagine synthase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000050">
        <property type="entry name" value="Asparagine synthetase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050795">
        <property type="entry name" value="Asn_Synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033738">
        <property type="entry name" value="AsnB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01536">
        <property type="entry name" value="asn_synth_AEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772">
        <property type="entry name" value="ASPARAGINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772:SF2">
        <property type="entry name" value="ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001589">
        <property type="entry name" value="Asn_synthetase_glu-h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="6 10 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 10 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="14">
        <location>
            <begin position="2"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="11">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="373"/>
            <end position="374"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for beta-aspartyl-AMP intermediate formation" evidence="13">
        <location>
            <position position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR001589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ80524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="577" mass="65352" checksum="F951E84CBE49AF3A" modified="2017-01-18" version="1">MCGIFAIHHKDNVNEYKGTALQLAKRIRHRGPDWSGNFIANNTILVHERLAIVGLDTGAQPLTTKDGSLSLCVNGEIYNHIQLREEFPQYPFQTASDCEPIIPLYEKYDVDAPKHLDGMFAWTLYDSKKDRMIAARDPVGITTMYMGRINSNKKTVYFASELKCLVDDCDEIIAFPPGHVYDSATDKITRYFTPNWLDETKIPSTPVDYKLIRETLEKAVRKRLMAEVPYGVLLSGGLDSSLIAAIAARETAKAMDATPGNPSSETELDVVNGKSLTGIDDQGHLHGNVGFSKIHSFAIGLPNAPDLQAARKVAKYIGSIHHEHTFTLQEGLDSLDDVIYHLETYDVTTIRASTPMFLLSRKIKAQGVKMVLSGEGSDEIFGGYLYFAQAPNREEFHKESVTRVKNLHLADNLRANKSTMAWGLEARVPFLDREFLQLCMNIDPSEKMINKERIEKYILRKAFDTKNEEEKAYLPDEILWRQKEQFSDGVGYSWIDGLKEYAEKAISDEQFAIKKPEWGDDQPTTKEAYWYRLKFDALFPQKAAASTVMRWIPKADWGCHEDPSGRFAKIHDQHVEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0V4</accession>
    <name>A0A1E5R0V4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein 72</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g4438</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0V4"/>
    <dbReference type="FunCoup" id="A0A1E5R0V4">
        <property type="interactions" value="103"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0V4"/>
    <dbReference type="InParanoid" id="A0A1E5R0V4"/>
    <dbReference type="OrthoDB" id="49520at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046757">
        <property type="entry name" value="YL1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13275:SF4">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 72 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13275">
        <property type="entry name" value="YL-1 PROTEIN TRANSCRIPTION FACTOR-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05764">
        <property type="entry name" value="YL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vps72/YL1 N-terminal" evidence="3">
        <location>
            <begin position="5"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="55"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="292"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="609" mass="69786" checksum="9CCAFE530D394379" modified="2017-01-18" version="1">MAESRSKRANAGNKLQKLIEQEKLLTESTKSLQTSFGAEIDGEDNDDLVNLLFQGQEDTEDQDFSINVKENEDDYFTDDDEGSGSSDEEAGEKALQEEEKKRKKRKTQQQVAAGTLTSGSKNAVPILRKRKATTLTASLELNQEGLNKRKKFSEELLNPENLLSEERRTSSRKSVVANKLKVYKNLQEAEKARKELHERLKHLNERKHVYVLTQEDRLREAKETEKLNLQSLNRFKELEDFNKQKRLLLQQRNKIKFQEDELVLTQLSCDWRMTPLMEVKDHEYWAEQIRKRESKNKRRKPTKKSTTPTLTLANAESLQVAKPLPTVDDLIKYDLTPNLLDEKSKESHNVSSDAPLEQNILDLAQQKIHTNTGAEAFNVEPSTHGSAEKKQLPQFDKDGNILIEGPEQRVSKNFVTLFKYDDFDSQAITKPVFMETMLGISPHLLQTKGKQSAQLASTFYDPENWETILHSKRKGDAEPMHIFKVDLLDNQEALCDRTMASFPRFGEFNKKFQVLETNETNVTEMIKLQTPAPTGLYLPNGQRKPCLIKGTSSQYFDPKFGIPYGDLDSYRLLQEMVQHPDLFKWYGLKNGGLLYNPNVKPASGVPEGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0Z5</accession>
    <name>A0A1E5R0Z5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein DSF2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4503</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0Z5">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0Z5"/>
    <dbReference type="InParanoid" id="A0A1E5R0Z5"/>
    <dbReference type="OrthoDB" id="3972755at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010972">
        <property type="term" value="P:negative regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052945">
        <property type="entry name" value="Mitotic_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43628">
        <property type="entry name" value="ACTIVATOR OF C KINASE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43628:SF11">
        <property type="entry name" value="PROTEIN DSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="326"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="360"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="613"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="755" mass="84106" checksum="2E7C3DD81585BD0C" modified="2017-01-18" version="1">MDKHAAKAKMSNEKSELWSPLDNIRNNLPNRAKVTTSKLQNSNYKRYSTSSFVSVSSLXTNNTLTNLADITNSSYTETDKSKLQEQNFDDERLLDNLNLSSYEEDILSKALQQQRQQEYQENRSAKEDLTRSSGFEWKENDSKMDQRNISSNSMFSHHSHAPTVCVPAAGFASLSVSGKPVKNVGRNRNRQGSVHQRNFSTGFINSTAANESAENIMSFDAPKLTAPLASIPNSHKRSESWSIQRSHINDVDTHEETVQVFNNVTDLMNXPSGKAEPKSKVGDNPNKTPKNPMSVVTPNASTTKFEFPAQAKQHEQAEQIHDNNYVKPRTPRFKTPTSSRHGSFSSGNTKAFFQNFSLTKSPSITPNKHHFSNQQSKKSTPYLHPPLHXDMNENTHEKPTFRHHKKKSSTFSIKNIFKASPKPSPFLDAAEVSSEDVLHSRETPQKKTGFVYVPPELPSPPRPPQFYSQTSFSSAGLGGKKLIESEPLVSSEDRIKLAIDLKFKGLYKESTDQLFMACQENDKMAFLLYGLALKLGTGIMPNPAESLRYIKRACGINNKEDEQTKIFDESAKLDPYELSFNHAEAVPDVPPEPLAPALYEAGLFYLKGPSGHNSQSSSPAMPNAPAVTPSSNSAHLSTKSPAFSASTASLSASSTRSSCSMAESFDXFEIDELRALRYLELSASLGNKDSILLCGSIWCKEKPAVRPKDLVRAASWFRIAESKGCRLTGSEWIYREKYMDKEKSTSIMKKRLSXT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1F4</accession>
    <name>A0A1E5R1F4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g4053</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat ELP2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1F4">
        <property type="interactions" value="1156"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1F4"/>
    <dbReference type="InParanoid" id="A0A1E5R1F4"/>
    <dbReference type="OrthoDB" id="27911at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001142">
        <property type="entry name" value="Elongator complex protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037289">
        <property type="entry name" value="Elp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44111">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44111:SF1">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="7 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="53"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="203"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="282"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="641"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ80726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="831" mass="94264" checksum="CEC86CCF170F3B6A" modified="2017-01-18" version="1">MQPSVSAIFTGANHQNQVSDYNKQKEIIAFGAGKNIALWRPLDQHYKGVYTTLKGHESEVTCCRFLSESLLVSTSEDFKIKIWEFDDNTDSNAKCIQTIDHYTHTVTTLDCDTQTGKVFATGSADGKVSIWCGSAASTQFELKHEFLIKKNVFPLCVSIQKIEETDAYLLAVGGTIASVFIYSFLINETNNFDIENFQQAACMEGHEDWIKCLDFRRDERLVYTLATGSQDRYIRLWKIDIAYKDKKIKEQDSKKLSLLSNKKYKFQVSSQISGAINFEALIMGHDDWVSSIKWHETKEQLLASTANTELMVWEPEEASGIWICNSRLGELSSKGASTATGSSGGFWSCLWFTQNNTEYVFTNGKTGSWRCWSCPLNSSLTVGDANNKTEEAEGETQTNDEEEDVLAFRDSSFKVWKSELAITGPYREATDVAWSNDGYLLATSLDQTTRLFSKWLLENSQEKFEAWKEFSRPQIHGYDMVCVEPITNTSFISGGDEKILRSFDEPRGVAQILDKFSDIHLNSESMPESASLPVLGLSNKATADAHIRDDEDQDVRETEENTNITYDLISSLTGPPVEDQLQRHTLWPEIEKLYGHGYEITCVDVSPDKQIVATACRSNTKQHAVIRIFDTTTWLQLKPVLPFHNLTVTRLRFSKDNKYLLSVSRDRQWAVWERNMADNSFVLKYKNEKPHTRIIWDAEWCPLEFDNAFITASRDKSLKIWSFDDNDFKLKNVIKLNEPITALSVLNSLVQGKVXVAVGKESGDTSIFSYDHENGFASVVAFDPAITPSXRNNRLRWRPVSQKNIEDPNLMLASASQDNSVRIYAVPKDLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2A5</accession>
    <name>A0A1E5R2A5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3948</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2A5">
        <property type="interactions" value="106"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R2A5"/>
    <dbReference type="OrthoDB" id="4034449at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070823">
        <property type="term" value="C:HDA1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038609">
        <property type="entry name" value="HDA1_su2/3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021006">
        <property type="entry name" value="Hda2/3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11496">
        <property type="entry name" value="HDA2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="163"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="408"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="467"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="169"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="514" mass="59329" checksum="94C6ADA9AB6D4072" modified="2017-01-18" version="1">MTITNHLYGLSPFQKDLLSVLEKSKPNDXVSPENVKETFRKFVFHPTLTVQHYIPRDLLLTTPLESLCFSSGCLHEFVKYLTQMATTNNDSVCIVTPSIQHLDLIERVLLSIPNESGGTHETQIKRLSGLALLPEHTFHIPTNEELEMLYYINKTKQERLRLEEEDKRRIGKNKKKKRKLDTGNKSGNGTSTPRESSVTAHGSSCNLGTFQSMLSNDIERLSYVSKYNYIMPLESNSAETVVAEKQQEKVFYLITSTHLSNLGVSQCLLESLQCRKVIYFEDDLAVEYPSSVDVQVFAYDFVDPMPLSISVNKDELLEYLKEMEDSLVFVRNKIREKQEKEFSRQHEMDQLRKDTRGMFKDKWNNKLEKEVEASSKKVYKVEDDMSRLMNYYDEISTFKSNASTGNTVSELAKEIETMRGQLQEQDDDIEKLRLQYQKVSNEAVSSTGAFNSVEKHLNFHKNKLNMLEHGMQETSFLEKEKANLEKXLSQMEDLANSDTYGKFVQVFEKRMTET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2F8</accession>
    <name>A0A1E5R2F8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable quinone oxidoreductase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">NADPH:quinone reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g4221</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2F8">
        <property type="interactions" value="622"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2F8"/>
    <dbReference type="InParanoid" id="A0A1E5R2F8"/>
    <dbReference type="OrthoDB" id="48317at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003960">
        <property type="term" value="F:quinone reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05286">
        <property type="entry name" value="QOR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000053">
        <property type="entry name" value="Quinone oxidoreductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047618">
        <property type="entry name" value="QOR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE PIG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106:SF13">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="5">
        <location>
            <begin position="16"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00070796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ81081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="335" mass="37217" checksum="AE1DE19A98F8ACC6" modified="2017-01-18" version="1">MSIPEKQSXVLIRETGGPEVLRYETDFPTPKIXNPNEILIKNKYSGVNFIESYFRKGIYPSEKPYVLGREALGVVVEKGAXVKDYNIGDHILYMSPGTFAQYTKVTAGVSKLIKLPQTSTQEELKTYAAALLQGLTVLTFVEEAYAVKKGDYILVWAASGGVGLIFDQILHKLGAHVIAVASTEEKLALAKENGAEYGILYDDPELLKKIEEITKGEGVDAVFDSIGKDTFETSLKAVKRKGSLISFGNATGPVDPLVLNRLSPKNVKIARPQVMAYIHTEEEFEYYAKKLIDYVKNGDLKIKIYKTYELKDYVQAAKDIESRKTTGKLLLEIPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2I7</accession>
    <name>A0A1E5R2I7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="2 10">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g3909</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="10">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with RLM1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="2 10"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2I7"/>
    <dbReference type="FunCoup" id="A0A1E5R2I7">
        <property type="interactions" value="437"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2I7"/>
    <dbReference type="InParanoid" id="A0A1E5R2I7"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07857">
        <property type="entry name" value="STKc_MPK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000013">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000647">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="26"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="448"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="472"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ81100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="546" mass="61909" checksum="D44C3EC82BA7E7CC" modified="2017-01-18" version="1">MSDTTNAIERHTFKVYNQDFTVDKRFQLIKEIGHGAYGVVCSARFMEAQEDTTVAIKKVTNIFQKTLLCKRSLRELKLLRHFRGHKNITCLYDVDIVFDGNNQFNGLYLYEELMECDMHQIIKSGQPLSDAHYQSFIYQILCGLKYIHSADVLHRDLKPGNLLVNADCQLKICDFGLARGYSENPVKNNQFLTEYVATRWYRAPEIMLSYQGYTKAIDVWSTGCILAELLGGKPIFKGKDYVDQLNRILQVLGTPSETTLRRIGSKNVQDYIHQLGFIPKVPFVTLYPDANPDALDLLERMLAFDPQQRITVNEALEHPYLSIWHDPADEPVCAVTFDFGFEAVNELEELKTMVVDEVRDFRSFVRQPLMEDADYEEEVIEEEELPQEQAHKAEQQFNFQQQKPLGEQNNTQSSAFQDLQHQQLAGLSSTNRGANSGSANVQESFIGISADNLPPHSTDLPPRPQETGYSEVPNNAYYNTPNSQPVSNATSANGSNPRGSIGDASNTTTTTTTNPHNSLLSDEDNVLSQFLDLEKELEFGLDRKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2L4</accession>
    <name>A0A1E5R2L4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Methyltransferase OMS1, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g4022</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. METL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2L4"/>
    <dbReference type="FunCoup" id="A0A1E5R2L4">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2L4"/>
    <dbReference type="InParanoid" id="A0A1E5R2L4"/>
    <dbReference type="OrthoDB" id="3971839at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000371">
        <property type="entry name" value="Methyltransferase OMS1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013217">
        <property type="entry name" value="Methyltransf_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050508">
        <property type="entry name" value="Methyltransf_Superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42912">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42912:SF83">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08242">
        <property type="entry name" value="Methyltransf_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Methyltransferase type 12" evidence="11">
        <location>
            <begin position="264"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="462"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="462"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="472"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ81145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="484" mass="55273" checksum="0B2054BE2522B47F" modified="2017-01-18" version="1">MLKVIHPVLRAGLLHRRLGAVSVNTLVASQSQLLHLRGLHQCRGLLQKRSRGGEVIPKSDAQRAAEAKALLEKQMNSSNKIVRWGAIARSEKFQKGLTKWLVGAYGLFILYGGYLMKNLYSREKELEDLKKRADELNEFEKLRSKELDKNVVLRTREEYKLKVYQLLKDEYGVSDFSNITIRDYEPAKDVVERVTGRSLPDDDEILLKFNKQNPLPNILPPIDTTGFYESKATEYDSDIGMEEKAVFMGSKRKWLMKHCSGDVLEVSCGTGRNIPYTDMGKLNSITFLDSSRKMLDVTNEKFRKEYPHFKKVAFVEGKAEDLLALNSDSGVKYDTIVEAFGLCSHQDPIQSLQNFAKMLKPNGRIILLEHGRSDYKMVNRFLDERASKRLDTWGCRWNLDIGEILDDSGLEIVEESRHHFGTTWCFVCKVKGSSKLQEEIGFVEKYISSSFKNRASAFIEDEKAKQTEKTNKPPSSHPQSSDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2N2</accession>
    <name>A0A1E5R2N2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein AHC1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4067</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2N2"/>
    <dbReference type="InParanoid" id="A0A1E5R2N2"/>
    <dbReference type="OrthoDB" id="5355528at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058707">
        <property type="entry name" value="AHC1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058706">
        <property type="entry name" value="zf-C2H2_AHC1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25910">
        <property type="entry name" value="AHC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25909">
        <property type="entry name" value="zf-C2H2_AHC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AHC1 N-terminal" evidence="4">
        <location>
            <begin position="144"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="AHC1-like C2H2 zinc-finger" evidence="3">
        <location>
            <begin position="252"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="205"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="453"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="115"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="55"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="205"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="241"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="466"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="476"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ81138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="559" mass="63810" checksum="5A9BC590E07D159B" modified="2017-01-18" version="1">MHKSSLKQSDINDRKKQLTLXLSPEKSNAMGEKPHINISNVVHTFEENNRFAQCNNDXENNNESKSINNTTRFHPHESSSAAFYYDITTPKSPHDESLLMYEXDTHSMADQSDDVDEKAMEREKLNRALLEEQDRLEQELKEREEKQVMFQVSKKEILNNIHLFMTMKYKENQMLKKSLETVNQNINLLEKLHQAKNLNLLSPNETNPVTDGAPQNVSSLKHTSTNQDQQAGLNQHHRRNYTNTCPTRNSGIVGKTSEGVPVFKRFDGIMIIIKCVTCGREGFTSPQGIVNHARLKHSKLYSSQQLAILNNQKLLPIQSQKILTKFKELKKDPNNDFLPLVAISLYPISIADSGKDLDRKLMLVEEKSSLPTQQKXMTSTHIQKHFSGKVETELPINNKSVLQQPKPLVSLRHLSNFQKNKESRSKENMEQSGNYDLINETVDFLKKFKPKSAIPSSQTTEGHFESSVSSFPSASATPNSDKSVPRSTASYIDEPETKKRKTVHGENGYLNYIKNDIKTQTSAKDLDQKPTLGSTFYNLRSRKRSSIPSKKWEDFVLYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2R6</accession>
    <name>A0A1E5R2R6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Putative mitochondrial carrier</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g4362</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2R6"/>
    <dbReference type="FunCoup" id="A0A1E5R2R6">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2R6"/>
    <dbReference type="InParanoid" id="A0A1E5R2R6"/>
    <dbReference type="OrthoDB" id="44467at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071913">
        <property type="term" value="F:citrate secondary active transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006843">
        <property type="term" value="P:mitochondrial citrate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049563">
        <property type="entry name" value="TXTP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788:SF5">
        <property type="entry name" value="AFR253WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788">
        <property type="entry name" value="SUCCINATE/FUMARATE MITOCHONDRIAL TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="94"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="228"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ80863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="329" mass="37518" checksum="642631AC8CB81F14" modified="2017-01-18" version="1">MPDTAHKEYDRALILTSSALASMITMTLSHPFEVLKIGQQLAFGNYQFNLVHKQLKLYSAGLSSLNLAISTKTFLRFPIYSYLCETLESPQGKISQSHILLAGSITGFLESCWYIPFENLKSRMIGNSIGKSEREQNVSLHNSPTALQKRIAVINARPLENQKKLEYYRLHPSINLFTSFKEICVTDGLKGFFRGSTPTVLRSMSSSAVNFMFYSIAQKNYMEHPYEHTYTLTTLIGIAASSAVVMVSQPFDYIKTRTQSNRYGPFYYNGVLNCIKTGLKEEGFKAFYKGWFPRFIKVNIINFGVQLGLYHYVESLLERNFNTQKFLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R3F7</accession>
    <name>A0A1E5R3F7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Quinidine resistance protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g4213</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. CAR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R3F7"/>
    <dbReference type="FunCoup" id="A0A1E5R3F7">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R3F7"/>
    <dbReference type="InParanoid" id="A0A1E5R3F7"/>
    <dbReference type="OrthoDB" id="3936150at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015203">
        <property type="term" value="F:polyamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010509">
        <property type="term" value="P:intracellular polyamine homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1720.10:FF:000009">
        <property type="entry name" value="MFS multidrug transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF5">
        <property type="entry name" value="QUINIDINE RESISTANCE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="503"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="543"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="585"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="611"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="651"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="675"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="126"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="349"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="57"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="351"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ81103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="717" mass="80353" checksum="84E950E38A15DE4C" modified="2017-01-18" version="1">MTLKNDVPESIVSSVMEELNSEDQAPDLQLQHKPTLLTHEKSDEKHAQASKCHKSNGRVSQSEQQKTAVEPPESLFQSISRAVTLKSSNKDLIVPRNKRNGLFSRILVLQQYKDARDYPRPIKSVLVFVVAAVSIAGPMGTSIIFPCIDSLEQDLDTTTDKVNIAVGIYLLGLGIFPIWWSSISEFKGRRTVYIISFSCLVGFSIGCALCKSIGAFIGLRLLCGASSSSAQTMGAGVLSDLFIPEERGAQIGLFYLGSLMAPLLSPIIGSLLVNRWSWRSTQWFMVIFAACNLLGLVLFLPETLRTINKKDVIKNLITETKQEATDNFAESQLNGRTKCSTTVSLEDGSYADEKGQEQEQHNAGNEEEEKEEEALVERVWTNNASVVSAYERRQEELEHLHSTLGTNHDNASNDEAEDDEIYPFDPVMPQMSKVRTNRSKKTVETHCSMKDEEHAKQRKILKHDVEENLEMQKKTGKLKNVDYKRLFYTYGLRPLKSLYYLQYPPVALSITFSAISFAVLYFVNITVEYTYSRPPYNFKPLYIGLLYIPNSVTYIIASIYGGRWVDRLLINYKKKHGILAPEARISYNVLVAVICFPISLLIFGWCLDKKTFWVCPLVGTALFGFASMMTIGATVSYLVDSVGSVGVAVNNLIRMILAAIACFTTDPMLKGMGVGWAFTMLAFIVLGSSSVLLILKWKSNYWRETLDLDALHRKVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4N4</accession>
    <name>A0A1E5R4N4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Mannose-6-phosphate isomerase</fullName>
            <ecNumber evidence="5 12">5.3.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g3689</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-mannose 6-phosphate = D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:12356"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="5.3.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11 12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the mannose-6-phosphate isomerase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.8" evidence="5 12"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4N4"/>
    <dbReference type="FunCoup" id="A0A1E5R4N4">
        <property type="interactions" value="839"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4N4"/>
    <dbReference type="InParanoid" id="A0A1E5R4N4"/>
    <dbReference type="OrthoDB" id="6605218at2759"/>
    <dbReference type="UniPathway" id="UPA00126">
        <property type="reaction ID" value="UER00423"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004476">
        <property type="term" value="F:mannose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07011">
        <property type="entry name" value="cupin_PMI_type_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.441.10:FF:000001">
        <property type="entry name" value="Mannose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.441.10">
        <property type="entry name" value="Phosphomannose Isomerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001250">
        <property type="entry name" value="Man6P_Isoase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016305">
        <property type="entry name" value="Mannose-6-P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018050">
        <property type="entry name" value="Pmannose_isomerase-type1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046456">
        <property type="entry name" value="PMI_typeI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046457">
        <property type="entry name" value="PMI_typeI_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046458">
        <property type="entry name" value="PMI_typeI_hel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00218">
        <property type="entry name" value="manA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10309">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10309:SF0">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01238">
        <property type="entry name" value="PMI_typeI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20511">
        <property type="entry name" value="PMI_typeI_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20512">
        <property type="entry name" value="PMI_typeI_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001480">
        <property type="entry name" value="Mannose-6-phosphate_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00714">
        <property type="entry name" value="MAN6PISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00965">
        <property type="entry name" value="PMI_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00966">
        <property type="entry name" value="PMI_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12 18" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phosphomannose isomerase type I catalytic" evidence="16">
        <location>
            <begin position="5"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphomannose isomerase type I helical insertion" evidence="17">
        <location>
            <begin position="169"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphomannose isomerase type I C-terminal" evidence="15">
        <location>
            <begin position="343"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="300"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="18">
        <location>
            <position position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001480-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001480-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF20511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF20512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ81852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="430" mass="47812" checksum="40E17173BA95C050" modified="2017-01-18" version="1">MSEQLFRLDAGFQQYGWGKIGSSSAVAQFAHHSDPSVKIDESKPYAELWMGTHSSVPSYNHDTKEPLSKIITEHPQEYLGSSIINKFESKTQLPFLFKVLSIEKVLSIQAHPDKALARVLRANDPKNYPDDNHKPEMAIAVTDFEGFCGFKPLETIADELNKIPEFKAVIGEELAAEFIANIKPSAAENSSEYESNRKLLQKVFGKVMNSKDDFIVEQSKLLIKRAQEHPECFTEKDLPALLLNLNEQFPNDIGLFCGGLLLNHCQLKAGEAIFLQAKDPHAYISGDIIECMAASDNVVRAGFTPKFKDVKNLVEMLTYQYGSVESQKMVPENFDRSSGQGKSLLYNPPIDEFAVVETTFKGAQGGVRSFKGLQGPSIVITTKGNGFLKNGDVKLRAEPGYVFFIAPGTEVDFISEDEDFTTYRAFVEAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4N9</accession>
    <name>A0A1E5R4N9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1748-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3557</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="interactions" value="1"/>
    </dbReference>
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    <dbReference type="InParanoid" id="A0A1E5R4N9"/>
    <dbReference type="OrthoDB" id="16824at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013726">
        <property type="entry name" value="Mitofissin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28075">
        <property type="entry name" value="CHROMOSOME 16, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28075:SF1">
        <property type="entry name" value="DUF1748-DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08520">
        <property type="entry name" value="Mitofissin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184603" description="DUF1748-domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="74" mass="8233" checksum="7724D2DCE4A2B742" modified="2017-01-18" version="1">MTLTGKLIHYSFDLMLVSTVLAGVRRQSGLQLKPDFFLQDEQAQKLVTKYLNFGESTLDYITATAGGSSSFVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4P5</accession>
    <name>A0A1E5R4P5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3814</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4P5"/>
    <dbReference type="InParanoid" id="A0A1E5R4P5"/>
    <dbReference type="OrthoDB" id="4066471at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="435"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="521"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="321"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="446"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="521"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="562" mass="63116" checksum="EB77103772BD75C3" modified="2017-01-18" version="1">MASKNESSLGKQLLTDCNYVSLLLLIDKIKPHLKVCSLRKNAWTMVLDHYNRSASIGYRQTRTIKNKFNKMRKFLETQELRTQLTVLFNDDSLMNLLEKLDQECKLFARDQIKAYYTEQEKLELHIHELNMKTNKEMKKQINENTNIADKLPIPISNNTSLGSSLHSPWSDGPWSSGNSSAKFDHVSPYYNHNEQTGAVPRKRSLDERDAENQYSGIDEDHHHSLPIQHQRPQYNKTAKASAIASPNSSANQLSPSSSTVSSLSATAFPRQPTYSTNLRVSQLRHGEVVNGGSTTETSAINNDMMTVTMLDSTTEQEGNDDNNSPSVVSASAKSPSQIEAPPLDSITVLPHTSFQKGQKKRQKQQQQQQQQDFGTTFSKNSPSPSNESNYSTPSNSNNTKLQGNHPQQFYSPNTFNNSSNDLLQTIINLVNQYGHTSASQPDHGSSKPSPLSQQQQLLDQQTGVSISAMQKLEIRSIIREEMRSFMADFQQAMEQKFSKQVEDLKTLILASSGVEFSLQQQTKSPLQQQTKSPLQQQTSFSLQQHQNELDRLNDTGSPKLPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4R4</accession>
    <name>A0A1E5R4R4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4R4"/>
    <dbReference type="InParanoid" id="A0A1E5R4R4"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="197" mass="22138" checksum="625E35E6E5C5F161" modified="2017-01-18" version="1">MSQQVMSNGTASIVNGAQTEKMNNNTLTTVSVPFNSKNDEARASSSTNVGNNETDVTIEDAPVVKQVGYSLRMERNVNIYDPVLRYLDKKLNVYKSPFSTRLYKISKPKSDETSSAVEKALNNSEILEKENLDRVQEKKDTRRSSGRDDDDDDDDDDDDDDQPEEIVSHDRRSKNLDNPMLTTNIHILSSDDETDGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R559</accession>
    <name>A0A1E5R559_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein uS5m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">28S ribosomal protein S5, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3654</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R559">
        <property type="interactions" value="632"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R559"/>
    <dbReference type="InParanoid" id="A0A1E5R559"/>
    <dbReference type="OrthoDB" id="309483at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000022">
        <property type="entry name" value="28S ribosomal protein S5, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000002">
        <property type="entry name" value="30S ribosomal protein S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_uS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_uS5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_uS5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48277">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48277:SF1">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8 9" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="S5 DRBM" evidence="10">
        <location>
            <begin position="148"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ82029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="310" mass="34828" checksum="E534D1329A5CF02F" modified="2017-01-18" version="1">MWKQCVRHQSTYNKSLASLYPSSSKLAQQVIPKDALFKQQPNVEFAPSYYDDFTKLDPYWDYVPGLPGRYRHMPSALPQWVPPDQKMPANYNIMKDSDKAQIPMDEMKRTGNAGAAGXGAKAAKDSTLFAKDIAKRTGVDEKYLLQNLKMKPLIVKPVKNTTAKGKIMSFYSLVVVGDGNGIVGLGEGKSRSEVSKAVQQAHRDAIRNLVKIPLYENRTIFSDLDYKFHGVQLHFRTKKPGFGLSVNHVIYEIANLAGIKDLSAKVYKSRNQMNIAKGTIEALTRGQKTLEEIALQRGKKVVDVKKIYYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R580</accession>
    <name>A0A1E5R580_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Prokaryotic-type class I peptide chain release factors domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3770</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R580"/>
    <dbReference type="FunCoup" id="A0A1E5R580">
        <property type="interactions" value="251"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R580"/>
    <dbReference type="InParanoid" id="A0A1E5R580"/>
    <dbReference type="OrthoDB" id="270639at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016150">
        <property type="term" value="F:translation release factor activity, codon nonspecific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070126">
        <property type="term" value="P:mitochondrial translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052104">
        <property type="entry name" value="Mito_Release_Factor_mL62"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11075:SF54">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11075">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110916">
        <property type="entry name" value="Peptidyl-tRNA hydrolase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prokaryotic-type class I peptide chain release factors" evidence="1">
        <location>
            <begin position="86"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="237" mass="27660" checksum="50701BB5D5700DC7" modified="2017-01-18" version="1">MRKSLFPVFFNICSKRCIGSSNWKCQTPTRQWDNFSQIRKQLCGAKPPALMLCRQMSGKIQSTNSEYTADDISKAQRWLSRLQPSSIPKHVYEQRFSRSSGPGGQNVNKLNTKCTLIFPNISKTGNCWIPNLVLNQLLHGNVKNEYKNFLLNNVYSEKKDALVIQSDRERSRELNKIDCLNKVVLLLQNSFFVQKEISQEDEQKWTNIKKSSNEHRLKKKKFLQDKKQSRKKIPLDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5A9</accession>
    <name>A0A1E5R5A9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nucleosome assembly protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3723</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the nucleosome assembly protein (NAP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R5A9"/>
    <dbReference type="FunCoup" id="A0A1E5R5A9">
        <property type="interactions" value="1706"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5A9"/>
    <dbReference type="InParanoid" id="A0A1E5R5A9"/>
    <dbReference type="OrthoDB" id="27325at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.90:FF:000003">
        <property type="entry name" value="Nucleosome assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.90">
        <property type="entry name" value="Nucleosome assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037231">
        <property type="entry name" value="NAP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002164">
        <property type="entry name" value="NAP_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11875">
        <property type="entry name" value="TESTIS-SPECIFIC Y-ENCODED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00956">
        <property type="entry name" value="NAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143113">
        <property type="entry name" value="NAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="207"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="372"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="434"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="60"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="210"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="380"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="434"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="478"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ82079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="491" mass="55520" checksum="8DC93D26290E0E11" modified="2017-01-18" version="1">MAGNKPIPNRNQKDFKIANAPTPHNTPSGDSTSFLNKSTSPTVGNGIGERSGKKEDKSVTNDSTTDSTNNSSATSAATTTTTTTKKTTTATDSPSTTTTATTKPSPLGLMQNEVLLQSIQDKLGDLVGQDSGYVNALGPQVKNRIYALKHLQAKLNEAEWEFQQQLYELERSFVEKHYKPIFEQRANLINGVVKPTKEEVANGKAIEKELAEEDDTDEDAEEDAEEETEETEDNTGVPAFWLTAFDNLPTVSQTITDRDAEVLEYLENVELQYLAHKDSSPLGFNLIFSFNSEENPYFTDNTLVKTYYYQNELGYSGDFVYDHADGCEIHWKGNDFNVTIEVETKKQRNKATKQIREIKKIIPVESFFNFFDPPKIPATEENDEKDDNDKEHEEDEDEELESRLALDYALGEEFKDKLIPRAIDWFTGVALDFEMDGMPQDEDEYDEEDEDEFDDNEDFDGEGEDDEQEEDEENDDFANTKKEQPPECKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5D9</accession>
    <name>A0A1E5R5D9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein DIA2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3582</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5D9">
        <property type="interactions" value="111"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5D9"/>
    <dbReference type="InParanoid" id="A0A1E5R5D9"/>
    <dbReference type="OrthoDB" id="629492at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904:SF523">
        <property type="entry name" value="STRESS-INDUCED-PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904">
        <property type="entry name" value="TPR REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="697" mass="81168" checksum="A773D3D285717C69" modified="2017-01-18" version="1">MSDADDLVHKVIDVGKKYYRTAEFKKACSMFTKAIVLNESFSESQLENIRLQYGLAKKPNHDHSRIWHPLLCQALDFRSLCYEKLKKYNESFKDGKAMINYEPYNMKGYIRCGKLYETSLEKPQRALKYYELGLRKAELGXSSFKIKPNKKLTQYLQQRXQXLKPMVPPASILPASRIXDXETLXKMKRKIEEPLDLVETXLLKTQKLQEQREVIDLREFDILNVLPVEIMNQIMRHITCKDVLNCMGLNRLWSQYIHCYSAPLLQNVYIRNLTDLKGFDKFLGKCSKFGDPIIIEKLHFNSSKLLGQKVLSMLFDILLSKTSIVRELAIIAYDLNLAVLTNYMEQNLTWCSHLQKITVICQFLPIVELEHRLLKMCDNLQSLEILYTEVSTSMSSHAIRDHNTNNKGKYTLEQHLSLRYLTIANLVYRKNRIPLVILPKCPALKELTLFGMKLSTDYFEGLNEKFPHIESLKLEKLKDFSLLSFLQIFSQNKMQFRLKKLTIREDKLILMPLLPINQAQRMTLAENFSDLRELDLYSSSITFHNLYKLIEQCFKLESLNIGNCPNLVFDNAGQDIITQIFDQLRNATNLKKLLVPQLIGINSIIFETLVIKQISMLPELQMLDISFNEEINGVNLWSLVRTLNNPNLILLCNGCSKIGPTTISQIQNTXSTPLVRKIIYEYTLQQWTRYVLRNYYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7F0</accession>
    <name>A0A1E5R7F0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Nicotinate phosphoribosyltransferase</fullName>
            <ecNumber evidence="3 9">6.3.4.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g3372</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate at the expense of ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>5-phospho-alpha-D-ribose 1-diphosphate + nicotinate + ATP + H2O = nicotinate beta-D-ribonucleotide + ADP + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36163"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32544"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1.</text>
    </comment>
    <comment type="PTM">
        <text evidence="9">Transiently phosphorylated on a His residue during the reaction cycle. Phosphorylation strongly increases the affinity for substrates and increases the rate of nicotinate D-ribonucleotide production. Dephosphorylation regenerates the low-affinity form of the enzyme, leading to product release.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the NAPRTase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.21" evidence="3 9"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7F0">
        <property type="interactions" value="194"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7F0"/>
    <dbReference type="InParanoid" id="A0A1E5R7F0"/>
    <dbReference type="OrthoDB" id="193380at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00457"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004516">
        <property type="term" value="F:nicotinate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034355">
        <property type="term" value="P:NAD+ biosynthetic process via the salvage pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.140.10:FF:000009">
        <property type="entry name" value="Nicotinate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.140.10">
        <property type="entry name" value="nicotinate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041525">
        <property type="entry name" value="N/Namide_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040727">
        <property type="entry name" value="NAPRTase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006406">
        <property type="entry name" value="Nic_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007229">
        <property type="entry name" value="Nic_PRibTrfase-Fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01514">
        <property type="entry name" value="NAPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11098">
        <property type="entry name" value="NICOTINATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11098:SF1">
        <property type="entry name" value="NICOTINATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04095">
        <property type="entry name" value="NAPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17767">
        <property type="entry name" value="NAPRTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000484">
        <property type="entry name" value="NAPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54675">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6 9" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nicotinate phosphoribosyltransferase N-terminal" evidence="12">
        <location>
            <begin position="14"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Nicotinate/nicotinamide phosphoribosyltransferase" evidence="11">
        <location>
            <begin position="176"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="195"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ82840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="450" mass="51478" checksum="83369EC40B800A18" modified="2017-01-18" version="1">MSSNQIXPVITSFLDTDLYKITMQAAVFTKFPEATVTYGYTNRSGADKKFTLDAIHWLQAQFQTLSLLKFTTEEIEYLRKTVSYLPEDYLEYIKTFQLNPQEQIQFTYSKVEGQNDLYDLQILVSGDWKETILYEIYILALVSESYFKFMDTDWDYDGQKEKAYQKAKKLLDNDISFSEFGSRRRRDCKTHDLVMQGIMQAVEEHQQENAQKQNDRKSAVAASSDSRKLFLGTSNVYYAKKYSVAPIGTVAHEWMMGIGSITGDYVNANKNAMKFWIDTFGPKNAGLALTDTFGTDSFLKPFHPPFSDYYIGVRQDSGDPLEYTKKIANHYLNVLKYPKFSKVVCYSDSLNVDRCIEYARACHXAGLNSTFGIGTNFTNDFQLKSDKATKSEPLNIVIKLLNVNGNHSIKISDNMGKNMGDPETVQRVKRELGYVERSWAGDNEAHRWAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7I2</accession>
    <name>A0A1E5R7I2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Guanine nucleotide-binding protein subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3378</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat G protein beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7I2">
        <property type="interactions" value="460"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7I2"/>
    <dbReference type="InParanoid" id="A0A1E5R7I2"/>
    <dbReference type="OrthoDB" id="10255630at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016346">
        <property type="entry name" value="G-protein_beta_1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001632">
        <property type="entry name" value="WD40_G-protein_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19850">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN BETA G PROTEIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25391">
        <property type="entry name" value="WD40_Gbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002394">
        <property type="entry name" value="GN-bd_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00319">
        <property type="entry name" value="GPROTEINB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="80"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="169"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="210"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="263"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="390"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="315"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ82838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="426" mass="47118" checksum="ECA8C1ED473ADEB0" modified="2017-01-18" version="1">MSLSMEDLQYRLQLARDETRQLHNQLXIIKNTNEDANLLGMSRTINSLLGTNQTGNISGTSTFLRSSLANANNMKNARVLRGHHNKIADMKWSRDFENIISASQDGYMIVWDPATGFKKNAVVLDNQWVLACAISPSSRLVASAGLNNHCTVYKVDNTSMIQQKTVSIFKGHTCYISECEFQSDTSIITASGDMTACLWDIPKSMKKTDFSEHLGDVLALALPKSGFHAENTFATAGSDGYVLVWDSRQKNSVQKFLVGDTDIGSLQFFSDGNSIASGSDDGVVRLFDLRADCKIADYSLVNKFQSLNKYHLQPPTEYRMTPHSEQSPFGSSKQQQNNDSASIRTKDSTFDTSGVVSFDFSVSGRLMYVAYSNYGCLIWDTLKGEIVGTLEGHSNRITKVKSSPNGFGVLTGSWDETIKLWTPSYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7U4</accession>
    <name>A0A1E5R7U4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3245</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7U4"/>
    <dbReference type="InParanoid" id="A0A1E5R7U4"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ82967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="268" mass="30336" checksum="DA5E35D76A6BD06E" modified="2017-01-18" version="1">MSCALPNDFLLPNYTELDLINKLQGLSRESILDLIKEWDKNAKSSTILESAEKIDKKYANRDSTFINVLVKTVLAAGLTAGQRADLERTLVLSNIGEIDEWSAYKIFDKKWDLPTAKYVSTNKLKTTLDKELIKRSKAFHISEQGIADARLKMLSVLIFDNYLEAELEKLSSGENFDELFKSCLITSLSKSSQLSAKQIILDGRSSNFTFSELYESELHDTHVAYDTILEPYIKHSVDIPLTTTDSQEGLRYSHLSYFFKQAPAKSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7W4</accession>
    <name>A0A1E5R7W4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sir4 SID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3198</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R7W4"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031556">
        <property type="entry name" value="SIR4_SID"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16991">
        <property type="entry name" value="SIR4_SID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sir4 SID" evidence="2">
        <location>
            <begin position="735"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="509"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="546"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="643"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1182"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1273"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="347"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="361"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="577"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="643"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="678"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1388" mass="153619" checksum="AD8BE2E3EB2FAF53" modified="2017-01-18" version="1">MPSKKEYMANAYQVNEKSGVPLSSTTRGKDVASPKRNKNSFHINEDEGLALLAQQAQKRKQQALNKMSHQKLQYKRLAKKSKNNRSGQXKTKIVDPFFNEQRQNLRLSSPATHTDKPGVLSSSPAPVSSFHVSTKPEKNERKQKNSXMQGPVFQXTQXTDNXXPVTQSSYKTVPSRTVERRLTNRDNTXRHYPNATXNXQNLRQPXVRQLQNIDTXSXDPPISQDXGGSAATLELEQSELIMSKQNDRLNRLFSRISGHNTALNKFQGNNRIKKRIWNEKPFGNTKFKXXAVXXTFNSNDDARLSNHPXALQHDTDIVMRNAKSKDTPXANNLTKNNARXXFVLGKGSEQGSEQGSEQESNTNHTSVKSTSNGSNQAQHEATKRLSAIEQKQDTPGLIQRNKLTKEVEVNTEENHLXEGSALKTEKSVGLSLPNSIESPVATKKYVLKDYPHSRETDFLSXFTDESDSEFVSSGFSPVKNISRXPFEVEENSLKNNIQSLKTTSNLKDGVTAATAGGPLKPNKSSELSFEAPRWDINKRSEQKLLKDDSTEIKNQYSKQRPSSPPPNQNQPGLSTLPEKNTPTQNSAQISFSQSSLPKMESILSSSSFSSRSPLVKNATTLPEEIKENTKGVAVPSVDIGISKSTKQPSLKNGLQNSNLGDIDTDTPLREKQKSRRLNINSIFSSSDESSVDEHDSNGNDVENTVXDLTEHASVADXKVGAAEPQPFMAKNASLSDLSSSSNVVEMVVPDQQYHRKSKHGIVNKVRDFVENYGVYNRMNDLNIKKMLHSKKQKLITKSDFTKTMLGGIVPHTPFLNSPRVSSTNNIVNVLNFQEESSHGLLKSADYFLKNDFIGGRXPSPTNQKKXNIAHQKSDNYSVTTCSKRFLQSSQSVKXETSKVSKPSLPTLVQILPVETLLSAHQTCSARELPGESSARSGLILDSAPVESNLGESVPKHPSFLDKRVSNIQLSDLPGKLFVDTLESDFEGHLMGSEKDANIVRFVSDSEKGIADTNQKPTSFEKPENGSAIIDLDTISLLQNNQKYVSEKSENVENMAHLRSSXSATNRLFVETEESDATEEEEKEEEEESIICKTDQDAQIRDLENFQNEENADTVAVTSFTGKLPSKAPFVLKQVLSSFELKNSQEDGQLSTKEHSFDTTNCEETVIKESVKTVLLPKSSFSNTNNIQRTNEKPLETNKGSTKPVVNTEKIVSQEERNQSFDHVLTQGLGINLAEPPSLSEIKENTKLSAEDSLIDVSDLSSINDSDLEELLSSDNSVSSVSEDTKTHRGMNTLKGNINADTPLEGTEAQAQEPVENRNTEPYISESQTSEEGFAAILALMTSHYSGLKSYFANKKVPFTKEEQNLLEQSKTILEKAKLVADKSNGVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7Y6</accession>
    <name>A0A1E5R7Y6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Inner centromere protein-related protein SLI15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3258</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the INCENP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7Y6"/>
    <dbReference type="FunCoup" id="A0A1E5R7Y6">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7Y6"/>
    <dbReference type="InParanoid" id="A0A1E5R7Y6"/>
    <dbReference type="OrthoDB" id="6123at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005635">
        <property type="entry name" value="Inner_centromere_prot_ARK-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03941">
        <property type="entry name" value="INCENP_ARK-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inner centromere protein ARK-binding" evidence="8">
        <location>
            <begin position="697"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="162"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="321"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="409"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="595"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="162"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="173"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="195"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="321"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="351"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="370"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="436"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="488"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="601"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ82981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="764" mass="85217" checksum="EEF6BC8C053E02B2" modified="2017-01-18" version="1">MDWALRVAKKRKESAAGGSRSIVESLTQLSQKKLTAVATLDEKYQSIDSWIDGKVEKINDFKKQKASAYSKTHGKTNHPDKEPVLKLLHDLPQSKVSFEPKPNPMLEAPSLRAPPQSFDLTLSNASRSYISPNTSRVSNSVAPTQSTHLVSLQNQLLPEKSAMSTQQLGTPTSEHEISEDIKRNVPNIKSSVGVVEKDSVENKNDDNGETKNKSPHSANTTLERKPLLSNRIKRRTNLFVPIPDNSAIMAQAATSFKADRLSIALQNSALGPVPSNANLSNLNEHEVEPIVEEKSLLVKHSQTTSPVPESNYRTLKISSSLKTRGSLVSSTKASVGSSFHEKRQQPQPNPNDEKTNVRSTTHEEAIPENVAQTNAQKSSIETQHSQKLPVSKTKTLDVFERLSQIPQKYFERSPNKRDLKRRAKNEKVNKTPAKRFQSSDQALALQRAQSRLGASTSGTPSSRVPITQKAQTAVKTKKTADFERSSLAPATSDRSLLTPSKPANGKPKTMSPIFQKKLVRQELSGLTPKSLATKTAETAQSKHNTATKPVVNNSTADKLTRFQLRTSSTTNASLGFNDRDDFKQKINKRLSNVVKTQQLQNEKRRLDHEKRKSQLEEETMKRRSKLFRDSMTKSSNLHSVSHQQQPYQQRLSMHAQSNSVLHDVNLKDYRLKAGKNYDAENFLSEDADDLENTTLPDIDSDSDVDERSNILKTWAHEPFLTEQLQRQSSIDPLNVFGRIPPLHTNEIFDTSRLSKFKSGSATGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R915</accession>
    <name>A0A1E5R915_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3467</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R915"/>
    <dbReference type="FunCoup" id="A0A1E5R915">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R915"/>
    <dbReference type="InParanoid" id="A0A1E5R915"/>
    <dbReference type="OrthoDB" id="978at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012901">
        <property type="entry name" value="CARME"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12303:SF11">
        <property type="entry name" value="AER338CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12303">
        <property type="entry name" value="CARNOSINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07942">
        <property type="entry name" value="CARME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01296">
        <property type="entry name" value="N2227"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184650" evidence="1">
        <location>
            <begin position="22"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="441" mass="50522" checksum="A1AE793B56B3769E" modified="2017-01-18" version="1">MINPSVLNVFVVLLVVAVSFMKNNKKVQNFLISKIETLLYKNLKTTRDLENSFSKVPVSFDSGLGNINPFFTNGAAYTLKDLYDGLCAFKMYEMKSLQYNNVIRKRYNSLPKAQFELVKTIKYGEKLDAVDNTVVEVNQVFMNSLITSYLENNKTVKQKLEEHDPELQLRTPINSNGIAFHRIVETLNHLVRDYSEDMFNMETKPLLEYIQSRLNTIKSLKSEANLNNGKTCIILPGSGVGRIAHEVATEHPNANVHSIEFSSLMYLANQFVYGGKETSSVSPFCNYYSNHLNTEDQLRKFELPLGAFTKPENLKAHYGDFTKFTLASDENEYQNIVIVTAFFIDTAENLLSYLDAIESFQRNLADNKKSTLHWINIGPLKYGTKPMIQLTKKELTLLRKERGWLDLQELCDTSKNIGYITDEKSLYKSYYSVLQFHATKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA15</accession>
    <name>A0A1E5RA15_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Diphthamide biosynthesis protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2999</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2 [3Fe-4S](0)-[protein] + 2 Fe(2+)-[Dph3] + NADH = 2 [4Fe-4S](1+)-[protein] + 2 [Dph3] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:71239"/>
            <dbReference type="Rhea" id="RHEA-COMP:17997"/>
            <dbReference type="Rhea" id="RHEA-COMP:17998"/>
            <dbReference type="Rhea" id="RHEA-COMP:18001"/>
            <dbReference type="Rhea" id="RHEA-COMP:18002"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:33723"/>
            <dbReference type="ChEBI" id="CHEBI:47402"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83228"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[3Fe-4S](1+)-[protein] + Fe(2+)-[Dph3] = [3Fe-4S](0)-[protein] + Fe(3+)-[Dph3]</text>
            <dbReference type="Rhea" id="RHEA:71235"/>
            <dbReference type="Rhea" id="RHEA-COMP:17996"/>
            <dbReference type="Rhea" id="RHEA-COMP:17997"/>
            <dbReference type="Rhea" id="RHEA-COMP:18002"/>
            <dbReference type="Rhea" id="RHEA-COMP:18003"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:33751"/>
            <dbReference type="ChEBI" id="CHEBI:47402"/>
            <dbReference type="ChEBI" id="CHEBI:83228"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DPH3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RA15"/>
    <dbReference type="FunCoup" id="A0A1E5RA15">
        <property type="interactions" value="344"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA15"/>
    <dbReference type="InParanoid" id="A0A1E5RA15"/>
    <dbReference type="OrthoDB" id="66964at2759"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.660.10:FF:000001">
        <property type="entry name" value="Diphthamide biosynthesis 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.660.10">
        <property type="entry name" value="DPH Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044248">
        <property type="entry name" value="DPH3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007872">
        <property type="entry name" value="DPH_MB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036671">
        <property type="entry name" value="DPH_MB_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21454:SF31">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21454">
        <property type="entry name" value="DPH3 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05207">
        <property type="entry name" value="Zn_ribbon_CSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144217">
        <property type="entry name" value="CSL zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51074">
        <property type="entry name" value="DPH_MB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DPH-type MB" evidence="9">
        <location>
            <begin position="4"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00036267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ83750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="83" mass="9684" checksum="C5D94AE7B882BEBA" modified="2017-01-18" version="1">MSSFYDEIEIEDMTFDNDTKTFTYPCPCGDKFQVYIDDLFYGDDVAVCPSCSLMIQVIFEKEDLKEYYEEADQTPPEPFMITA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA99</accession>
    <name>A0A1E5RA99_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Alpha-1,3-glucosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2555</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>an alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate = an alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31307"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19502"/>
            <dbReference type="Rhea" id="RHEA-COMP:19521"/>
            <dbReference type="Rhea" id="RHEA-COMP:19522"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57525"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:132521"/>
            <dbReference type="ChEBI" id="CHEBI:132522"/>
            <dbReference type="EC" id="2.4.1.265"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:31308"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the ALG6/ALG8 glucosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RA99"/>
    <dbReference type="FunCoup" id="A0A1E5RA99">
        <property type="interactions" value="797"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA99"/>
    <dbReference type="InParanoid" id="A0A1E5RA99"/>
    <dbReference type="OrthoDB" id="1689333at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042283">
        <property type="term" value="F:dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004856">
        <property type="entry name" value="Glyco_trans_ALG6/ALG8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12413">
        <property type="entry name" value="DOLICHYL GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12413:SF2">
        <property type="entry name" value="DOLICHYL PYROPHOSPHATE GLC1MAN9GLCNAC2 ALPHA-1,3-GLUCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03155">
        <property type="entry name" value="Alg6_Alg8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="188"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="220"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="376"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="441"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="466"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="505"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="537"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ83817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="567" mass="65924" checksum="622AE16FF3109361" modified="2017-01-18" version="1">MGHSAKQKNKKSPVKSPPLKKPLLQQIEVDETRNYSLWNFWVASTALKILLFPGYYSTDFDVHRNWMAITKKLPLKEWYFEKTSQWTLDYPPFFAYFEWFLAQFVPKAAIEDGCLDIVSVGMFGIPTIYYQRCTVILSEIVLFVVLQVYVNKSKMSEKSANFVVASSIVLSPGFLIVDHIHFQYNGFLFGILIASIVSAKFEHYLLCGFFFSVALCFKHIFLYLAPSFFVFLLRAYILNFDSSFKFKSYKNLFELVHWTRLFKLAGVVLSTFAVCFLPFARDYEQLLSRLFPFNRGLTHAYWAPNFWALYSFVDKILTVAVMKISQHTNKLDFLFKKCHLFTHWGHNNLDEVINTGTRGLVQDVTFEILPQIPPKITFTLTLFYQVIAVLPVLFAPTFKRFIGSLTLCGYSSFLFGWHVHEKAIMLVIIPFSFLVISDRRLLTPFRLVTAAGYVSLFPLLYTSQDFLLKILYVTIWCLIYFVALRDITKISSSIERRVWFFDRMAIVYIITLLPLIVACGTIDIMRVSEKFEFLSLMLYSVYCALGVGSSWLGLSWLYNFDEPLWEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAB5</accession>
    <name>A0A1E5RAB5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">2-oxoglutarate reductase</fullName>
            <ecNumber evidence="4">1.1.1.399</ecNumber>
            <ecNumber evidence="5">1.1.1.95</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g2925</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(2R)-3-phosphoglycerate + NAD(+) = 3-phosphooxypyruvate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="EC" id="1.1.1.95"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(R)-2-hydroxyglutarate + NAD(+) = 2-oxoglutarate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49612"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.399"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.399" evidence="4"/>
    <dbReference type="EC" id="1.1.1.95" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAB5"/>
    <dbReference type="FunCoup" id="A0A1E5RAB5">
        <property type="interactions" value="661"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAB5"/>
    <dbReference type="InParanoid" id="A0A1E5RAB5"/>
    <dbReference type="OrthoDB" id="1621027at2759"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00196"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061759">
        <property type="term" value="F:2-oxoglutarate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004617">
        <property type="term" value="F:phosphoglycerate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04901">
        <property type="entry name" value="ACT_3PGDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12176">
        <property type="entry name" value="PGDH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000036">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000041">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050857">
        <property type="entry name" value="D-2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008759">
        <property type="entry name" value="PRK11790.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42789">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G10090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42789:SF1">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G10090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="8 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="domain" description="ACT" evidence="15">
        <location>
            <begin position="400"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ83846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="470" mass="51282" checksum="4D7258B0BC19D5FE" modified="2017-01-18" version="1">MTSPQQINKLNNNFQDALNLSGSAGAVSTSPVQSFMNTLPHRQSISKQTKALKPFSTGDIKILLLENVNQTAVEILTEQGYQVEFHKTSLDDETLISKIKDVHAIGIRSKTKLTEKILKHAKNLVTIGCFCIGTNQVDLEYAAKIGVAVFNSPFSNSRSVAELVMAEIVALARQIGDRSIELHTGTWNKVSNKCWEVRGKTLGIIGYGHIGAQLSVLAEAFGMRVLYYDIVTIMALGSARQVSTLDELLSKSDFVTCHVPETPETKNLLSFPQFAAMKDGSYVINASRGTVIDIPALIQAIKVGKIAGAALDVYPHEPPKNGAGLFVDNLNPWISDLVSLPNVILTPHIGGSTEEAQSAIGVEVATALFRYINEGNSVGSVNFPEVSLRSLGYDQEKTVRVLYIHHNVPGVLKTVNNILSDHNIEKQYSDSQGEIAYLMADISNVNQSDIKDIYDQLNATEAKISIRLLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAR3</accession>
    <name>A0A1E5RAR3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor IIF subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">TFIIF medium subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">TFIIF-beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g2585</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIF beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAR3">
        <property type="interactions" value="529"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAR3"/>
    <dbReference type="InParanoid" id="A0A1E5RAR3"/>
    <dbReference type="OrthoDB" id="26094at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005674">
        <property type="term" value="C:transcription factor TFIIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07980">
        <property type="entry name" value="TFIIF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000035">
        <property type="entry name" value="General transcription factor IIF subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003196">
        <property type="entry name" value="TFIIF_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040450">
        <property type="entry name" value="TFIIF_beta_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040504">
        <property type="entry name" value="TFIIF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011039">
        <property type="entry name" value="TFIIF_interaction"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10445">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10445:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02270">
        <property type="entry name" value="TFIIF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17683">
        <property type="entry name" value="TFIIF_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50916">
        <property type="entry name" value="Rap30/74 interaction domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="14" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TFIIF beta subunit N-terminal" evidence="13">
        <location>
            <begin position="58"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIF beta subunit HTH" evidence="12">
        <location>
            <begin position="294"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="371"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="415"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="123"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="14"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="371"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="415"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="461"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00081473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ83961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="475" mass="55865" checksum="8E78543C1A8F559B" modified="2017-01-18" version="1">MSDKEDEIRPSRDNEDDPNTDDELLDEQDDIIDGNDIENNEKKVYEESLDLDLEGGKNQIWLVRLPMFLAEKWRNMDNLKGQELGKIRINRRTQEIQMILNEDDKYMKGLDEMPHAYDIELTKKVVENEFIFTEQNLKKYEQRAQQLEQNPELQKQLYILKKERQEEFEKKKKKQQQQYKRNRKKMFKQRVMIDRDGRDRYIPYVKTIPKKTGLAGTVCHECQVIPSMEDPNYIKIIEQRRELIRNNTKPKITVLEEGAGKTMSKAGLSMRSDNSNFLKVAKEKSKDKNTARAIRMPKKELLDLLFKLFDEYDYWSLKGLKERTKQPEVYLKECLDQVAMLVKKGPYALKYTLKTEYKKLKQAERNLRLGELADGHETDEVNVKPEDAEAENTLGVTTTLDNSALQDEQIINNNMINRNDPRNNNNNNNNSNXNSNNNNNNNYTGRDQNYYNNGNNNNGQGDDELDEDIEMEDVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAT2</accession>
    <name>A0A1E5RAT2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="10">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g3129</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="10"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAT2">
        <property type="interactions" value="813"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAT2"/>
    <dbReference type="InParanoid" id="A0A1E5RAT2"/>
    <dbReference type="OrthoDB" id="3971734at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10 14" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="187"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="398"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="704"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="14"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="58"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="723"/>
            <end position="736"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ84009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="755" mass="85032" checksum="82B3C7501872845D" modified="2017-01-18" version="1">MSEDDGMMLMNIAVPTSTASSVPSNNNKKVTGGKWKDRRKLERRLNGKPAPPRKQHFSDANSSTVVPANTYEQRRAQEAFKKQQKLASPDNTRTANPSHDNAPQEQVKPMKVDKQIVSSLFTSSKEIATNVNTHQHDGASHKASNAPLAGNDFSSLGITSKQLLXNISKKLNIETPTKIQKYAIPELLNLQEXKDLFIHAQTGSGKTLAFLLPILENIISTNNGKIDRNSGCFAMIITPTRELASQIHQVFQSISVHYLVPCLLIGGEKKKSEKARLRKGCNVIIGTPGRILDHLTNTRVIKDQLHGLKYLVLDEGDRLMELGFEESLKDIFKVIHEESSQNIKKVLCSATISDNVGRLGESILLKDYKIISSGKKNKKGHIVDQEEVENKLTTAPDQLLQKIVIVPPKLRLVTLAGCINNITTTSKFQDEKTIRTIVFLSCADSVEFHYNVFAHGDDNHIKTLINDSAISTTQGNTILPSLQNGDYQNVEYVFYKLHGSLSQQSRTSTLNNFINQSKTSDKKKHLVMFCTDVASRGLDLPQISTVVELDPPFAVEDHLHRIGRTARAGVKGESLLFLLPGEEEGYMDYIKPYHPKGWDLLKYDXVLKKCFHDSNVIKSDGKRRTEEEAEKEEAWDNNATTWHLNVERRVLDDPRFKTIATNAFLSHIRAYATHISKEKPYFNVKYLHLGHLCKSFALREKPKTLQFQKRGGKPNDDDDEDRDSNSRKRFKGDSKSRMLKMANMVQNQLSSEFNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAW8</accession>
    <name>A0A1E5RAW8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA annealing protein YRA1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2677</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAW8"/>
    <dbReference type="FunCoup" id="A0A1E5RAW8">
        <property type="interactions" value="1158"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAW8"/>
    <dbReference type="InParanoid" id="A0A1E5RAW8"/>
    <dbReference type="OrthoDB" id="346839at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051229">
        <property type="entry name" value="ALYREF_mRNA_export"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19965">
        <property type="entry name" value="RNA AND EXPORT FACTOR BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19965:SF35">
        <property type="entry name" value="RNA ANNEALING PROTEIN YRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="94"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="119"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="131"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ84048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="146" mass="15955" checksum="14CE193D2E17BEAD" modified="2017-01-18" version="1">MFEGMKLIDLDKLDGEFFQQAIGGVVSVMLSYNEKGVSTGFATVTFKNNDRAAKAVQELNGAPIDNNRRKLRIQLIVDPTKSAAPSNLGQSLAARIGTPVSKPVQKKKPAPRAVGKKQPVQKKKPAKAPKKSLEDLDREMADYFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB50</accession>
    <name>A0A1E5RB50_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="15">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">AWRI3579_g2637</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 25">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="15"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RB50"/>
    <dbReference type="FunCoup" id="A0A1E5RB50">
        <property type="interactions" value="1319"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB50"/>
    <dbReference type="InParanoid" id="A0A1E5RB50"/>
    <dbReference type="OrthoDB" id="10248617at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.270.10:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.150:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000011">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1110.10:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1800.10:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007646">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007647">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007175">
        <property type="entry name" value="PRK09606.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04566">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04567">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15 23" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="19">
        <location>
            <begin position="41"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="18">
        <location>
            <begin position="220"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="20">
        <location>
            <begin position="481"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="21">
        <location>
            <begin position="580"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="22">
        <location>
            <begin position="682"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="16">
        <location>
            <begin position="754"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="17">
        <location>
            <begin position="1127"/>
            <end position="1219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF04566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF04567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="OEJ84128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1225" mass="138973" checksum="DB6D30B78FD454B6" modified="2017-01-18" version="1">MSDYEEDNYMNDDQYDFSDENGPITAEDSWVVISAFFREKGLVSQQLDSFNQFISFTMQDLIVEDSSLILEQPAQHTTESDNISRKYQIIFGKIYLAKPSMTESDGVAHAMFPQEARLRNLTYASGLFVDIKKRTYEAVDVPGRELNYKLVEEEADEENAESNKIFIGRVPIMLRSRYCLLYDQTESDLYKLKECPFDMGGYFIINGSEKVLIAQERSAGNIVQVFRKSAPSPISLIAEIRSVIERGSRFISTLQVKMYGREDSANRTIKATLPYIKQDIPIVIIFRALGIVPDGEILQHICYDVNDWQMFELLKPCVEEGFVIQDRETALDFIGRRGATIGIKKEKRIQYVKDILQKEFLPHVTQLEGFEGRKAFFLGYMVNRLLLCALDRKDQDDRDHFGKKRLDLAGPLLAQLFRTLFKKLTRDIFRYMQRSVEEAKDFNLKLAVKATTLTAGLKYALATGNWGEQKKAMTSRAGVSQVLNRYTYSSTLSHLRRTNTPIGRDGKLAKPRQLHNTHWGLVCPAETPEGQACGLVKNLSLMSGISVGSDPVSIITYLKELAMEPLEDYVPYKHPDVSRIFVNGVWHGIHRHPEKLVKAIVGLRRSGELSAEVSVVRDIREKELKIFTDSGRVYRPLYVVDKNSDSGEIELKIKKKEIYNLIEAENQEIEGGLDDDTELTTWSSLLNNGMVEYLDAEEEETVLIAMQQEDLDPNFGANQDEEDLDPAHRIKNIRHSSTFTHCEIHPSMILGVAASIIPFPDHNQSPRNTYQSAMGKQAMGVFLTNYNFRMDTMANILYYPQKPLGTTRAMEYLKFRELPAGQNAIVAIACYSGYNQEDSMIMNQSSIDQGLFRSLFFRSYMDQEKRIGMSITESFEKPHRTNTLRMKHGTYDKLDDDGLIAPGVRVSGDDMIIGKTTPVNPDNEELGLRTAFHSKRDASTSLRSTESGIIDQVLITTNQEGLKFVKVRVRTTKVPQIGDKFASRHGQKGTIGITYGREDMPFTAEGIVPDLIINPHAIPSRMTVAHLIECLLSKVAALSGNEGDASPFTDITVDGISQLLREHGYQSRGFEVMYNGHTGKKLMAQIFFGPTYYQRLRHMVDDKIHARARGPVQVLTRQPVEGRSRDGGLRFGEMERDCMIAHGAAAFLKERLMEASDTFRVHICGICGLMSVIAKLKHNQYECRGCKNKIDIYQVHIPYAAKLLFQELMAMNITPRLYTERSKDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB83</accession>
    <name>A0A1E5RB83_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitochondrial distribution and morphology protein 32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g2650</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in the organization of the mitochondrial membranes and the global structure of the mitochondria. Also required for mitochondrial distribution and mobility as well as for the maintenance of mitochondrial DNA nucleoids structures.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MDM31/MDM32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB83">
        <property type="interactions" value="43"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RB83"/>
    <dbReference type="OrthoDB" id="17678at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012571">
        <property type="entry name" value="Mdm31/Mdm32"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31068">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31068:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08118">
        <property type="entry name" value="MDM31_MDM32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="407"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="502"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="414"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="512"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ84155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="652" mass="74793" checksum="EFB16A62B498AA55" modified="2017-01-18" version="1">MLKYLGGVLRNSRFGPLVRNRELILSRVLLTQSFAYHYKGPQXERFIFSQEYSKSCRKPSTLNTSKPSTSINSQIQILQSTLKTELLKSQGSKRPTKEELLSDADGFLDRFKIRSKWFLIKDNRPFNIDELGTLFSWFFLSQIIWFVLGTTTFVSLLLFSINAILGTNKDFVSMILNKSWGSKNISFDIMGDVLPNWTKNCISMKNLKIKTTRDKITATTQEEIKGETPPVSFDLSINTVEITLSLRKWLKGNGLIDAITIIGVHGNIDLIEDPMLSNWIKNKNYCINKIVVQDANILYNQTYEVAIFNMRLPKFRFDNTIHDLLSSSVISGAINNSLFTLHKRQHNLASIQDAAKDISSSWERITRIHLDQIPVKQLGLPNSKSFSWIDDGELEFTADVMIPKEPPEAEEQHTSTGYNNDYSDPVTTVNNEDREKDTATNNKYLVIDLKLKFKDLKAKIPDMPPHLSTNEIICKLDELRPLVEYVNSKRSRFLSKQQTYNSSYIKNDGPDNTSSNASQNTSQEDHMFPTFQNLFNENDSHSATFPQITIKSWPEDEDSETAGKEFIKYHNVDSNNNNEIILTCRVVANTQSLKGKTLLKETKIYDAITMELYYDLMKMVEDWEYKHTHDWKRLWTATIMSQLLLMGLGSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBC4</accession>
    <name>A0A1E5RBC4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2982</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBC4">
        <property type="interactions" value="107"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RBC4"/>
    <dbReference type="OrthoDB" id="4033270at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020366">
        <property type="entry name" value="Ies5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17335">
        <property type="entry name" value="IES5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ84200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="144" mass="16720" checksum="20608E88AECA72A8" modified="2017-01-18" version="1">MDGKNLDXLQKELIKVTNKKEELSKQFVSLSKDYTTLTRKVSSLYRIFETLDEAGTKLYYDVEEDTANNKEADQQIISQXVIERVPQLEMYNGVLXEIDTWQSDEIIDIKDIVKDLDLPIDLSTMVEMHDNTALLYKDSLNYVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBD9</accession>
    <name>A0A1E5RBD9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2472</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBD9"/>
    <dbReference type="InParanoid" id="A0A1E5RBD9"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013726">
        <property type="entry name" value="Mitofissin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28075">
        <property type="entry name" value="CHROMOSOME 16, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28075:SF1">
        <property type="entry name" value="DUF1748-DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08520">
        <property type="entry name" value="Mitofissin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="110" mass="12550" checksum="46B9A2CC6629CC15" modified="2017-01-18" version="1">MRTVNFTVTWSIIRHTVDLAAIAIILAAIKKQTGYTLSFGPNNEFNHLAKMYTSLGSYMYEKMLGWVRRSSYFKKTNIVEDFADNVSKTFTQLGTDVTDNVVRPTQLECD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBM1</accession>
    <name>A0A1E5RBM1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2736</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBM1">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RBM1"/>
    <dbReference type="OrthoDB" id="4068713at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="317"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="395"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="611"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="787"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="85"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="622"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="787"/>
            <end position="798"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="858" mass="95558" checksum="16B5AE94F9FC3D1C" modified="2017-01-18" version="1">MSNGKNIKTEQESEDXNAINGVERVEQVPTIQKTQQEELQQLEQLKNLNHQVTHNGQFHKHSSTSTTPVXSPKTAFLTADDSASTNTNTNTTYATTNKNNIKDKRKHQNGSNFLHTGARRSSQPYTSQSFSDPQNYPSIIQINNIPVGEKWKQIKYYIGGCIHHTNILEGMVLPYYQQPDPANSKSCLVLFKTPAVAQEAFYKLNGAVWGNNYLTVFYYGFANEQALPQGNIEQQQAKVSRPSHLYSSDYIDNPADEHGQKTTSRRSSVESSLEEFSKPKKQSDSKQVNLPTQKQHLQGVPYPMQQPMFVYYPASPEKYGPSDDENFSQKLKTTPTRNSGQGEFRVGTNKGQSWQPSASVPFQMLPQFPLQTFPPAPFPSSQPILPNVFAEQQQPSTVPALVESTNEPNEAALTSSSEKSPENLRSSTMETSTAQSQQRQPRYRAGSTGGDSRSRTSSGPLAAPFVPILQPAYYDPAKNAPVHLTHASPKNATSHNNGLMPPANVVPGELPVYQYVGAPPMGISHYPGMAPPYPFPVPHPSSPSTLSLGSSSSPISSNPANSHGMYFAPGFPMQQSPFYYPPLSPQYQQGLXQHYYPPHRQSPRSYACGGQNAMFSPQRASRGGGGGGGEPVGGPGSVPSQEFSERSFRSQMDVRGMQYQLKLSNFPYDYKIRWKLLKDFIKDDCIGVRMQETLATLEEKSGQEDTLVLGKXFSAQKYDLYVGIYKERLGEEDDVFVYDXKDVEDVLRKEGSGIKLCVGEETSRNFKQSDIENGSLAQMHHFVKNEKSKEEEWKKDNQNGEXDFMTRSIGERDPDVVVFKAILGFHEKELMDKVIAQLPVLHFIIPGCHLHYKVLSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBW6</accession>
    <name>A0A1E5RBW6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">Crossover junction endonuclease MUS81</fullName>
            <ecNumber evidence="16">3.1.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AWRI3579_g2668</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15 16">Interacts with EME1 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, D-loops, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication fork intermediates. May be required in meiosis for the repair of meiosis-specific double strand breaks subsequent to single-end invasion (SEI).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with EME1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the XPF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.22.-" evidence="16"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBW6">
        <property type="interactions" value="213"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBW6"/>
    <dbReference type="InParanoid" id="A0A1E5RBW6"/>
    <dbReference type="OrthoDB" id="5963188at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048476">
        <property type="term" value="C:Holliday junction resolvase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048257">
        <property type="term" value="F:3'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21036">
        <property type="entry name" value="WH_MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20074">
        <property type="entry name" value="XPF_nuclease_Mus81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000307">
        <property type="entry name" value="Crossover junction endonuclease MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10130:FF:000005">
        <property type="entry name" value="crossover junction endonuclease MUS81 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.670">
        <property type="entry name" value="Crossover junction endonuclease EME1, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.110">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027421">
        <property type="entry name" value="DNA_pol_lamdba_lyase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042530">
        <property type="entry name" value="EME1/EME2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033309">
        <property type="entry name" value="Mus81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047417">
        <property type="entry name" value="WHD_MUS81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047416">
        <property type="entry name" value="XPF_nuclease_Mus81"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13451">
        <property type="entry name" value="CLASS II CROSSOVER JUNCTION ENDONUCLEASE MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13451:SF0">
        <property type="entry name" value="CROSSOVER JUNCTION ENDONUCLEASE MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21136">
        <property type="entry name" value="WHD_MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47802">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 16" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="12 16" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 16 19" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0469">Meiosis</keyword>
    <keyword evidence="6 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 16" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC4" evidence="18">
        <location>
            <begin position="404"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="118"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="279"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="134"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="151"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="279"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="290"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00058015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU369042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OEJ84387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="687" mass="78362" checksum="E419A1FC1BB04919" modified="2017-01-18" version="1">MQIAQEERGGKNKFELESNSNRVQALKMSSRLRDECCKALEVLIENMDSKRQEKSVMVFNKALRQLKGDLSTKVENFNDILKVRGIGPAIQKAIYKQLNSENKLEELGIPRASFDKSSELPXSRLKRTKNSKEDSDDMMEDENEEDENATKKKRTHKKRKYVPKSRSGGYAILLALLSQFYKYPHGMTKNEIIDHAIAFCDLSFQSNPSTNEYYNAWSSMKTLINNEVVAELNVNKKPQRFGLTDDGFELADTLKKVNNVVFDNEKDFSRKWHAKTSVSTSAKTSNSAKGNNPNSSSLTAGNTSDASIGEVSEFSANLSLLLQKEKETLNQPLPKARLWKTQSILQDLPKKDDLFSTQDSVSNKNIQGLERNIEPLCGYADNIIRARYNGVSYEVWDIDSFDIILCIDNREIRARAQRDYFATTLAAKGVHTXIRNLHLADFVWIGKHKKTGKECCLNYFIERKRLDDLSDSIKDHRFTEQKHRLQKLNARCVFYLVEEITALDLGAMAEAVKTSMWQTIIYSGFHVQRTKNSEDTVVTLKNINDVLVKKFKKTKLLVIKPSYFENQDAYAQILEDFRAQFERKKEAGLECCHTFDSYQNIMGKTNLLTVKEAYLVCLLSVKGVSLEKALGIQSLFPTLKSLLLAYQKCKDEVEGKKMIMKAMEEFPGNKKVKKALSETLYNVFGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBY9</accession>
    <name>A0A1E5RBY9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3081</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBY9">
        <property type="interactions" value="145"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBY9"/>
    <dbReference type="InParanoid" id="A0A1E5RBY9"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19071">
        <property type="entry name" value="AKR_AKR1-5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000002">
        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF13">
        <property type="entry name" value="ALDO_KETO REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="5">
        <location>
            <begin position="24"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="4">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ84093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="294" mass="33378" checksum="EAAA054CB70C5732" modified="2017-01-18" version="1">MSTKHSVIPFRLKLNNGTLIPSVGFGTYNLPPTQTTELVYQACLKGYRHFDTAVLYENEKEVAAGIAXYIKEQSEAQKSSPESIRKELFYTTKLWNSQCGYSQAKKAIEGCLQKAGPLGYIDLLLIHSPLCGPKKRLETWAAMQEYVDQGKIKNIGVSNFGAKHLDELLGWKDLKYKPVVNQIEISPWLMRQELCDYCKAHDIQVEAYAPLTHGYNIDNPVVTKIAYEKEVTNGQILIRWSMQKGYIPLPKTANVSRLSGNLDVYDFELSDEQMKELDQPGEYQPTDWECTDCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RCF0</accession>
    <name>A0A1E5RCF0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Benzil reductase ((S)-benzoin forming) IRC24</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2461</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RCF0">
        <property type="interactions" value="118"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RCF0"/>
    <dbReference type="InParanoid" id="A0A1E5RCF0"/>
    <dbReference type="OrthoDB" id="153074at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004757">
        <property type="term" value="F:sepiapterin reductase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051721">
        <property type="entry name" value="Biopterin_syn/organic_redct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44085">
        <property type="entry name" value="SEPIAPTERIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44085:SF2">
        <property type="entry name" value="SEPIAPTERIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ84263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="289" mass="32018" checksum="44641543F98C7F0A" modified="2017-01-18" version="1">MTKQRDLVIVTGCTKGIGAAIVDELFAQCSQNKESPAVSVLGIARSRILLXQMSEKYGEDNFSYVCGDVTQEQTVQEMCDFVSAKCTTDNGSSEHDTLFLKSVVFNAGVLSPVSTIDASSADIEGWKKLYDVNFFSIVNMTMRLLPIFEQSRAHRKNQSVQNFVFVSSGASTKPYKSWGAYGSSKCALNHFASTLAAEQADNVTESAESSVPLVRSVSVAPGVVDTEMQEDIRYKFDTLMPRDFHNRFTTLHKNKELLPPQIPAKVYARLALNDIPVELNGKYLRYNEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RCH1</accession>
    <name>A0A1E5RCH1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Outward-rectifier potassium channel TOK1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3118</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RCH1"/>
    <dbReference type="FunCoup" id="A0A1E5RCH1">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RCH1"/>
    <dbReference type="InParanoid" id="A0A1E5RCH1"/>
    <dbReference type="OrthoDB" id="297496at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030322">
        <property type="term" value="P:stabilization of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF342">
        <property type="entry name" value="OUTWARD-RECTIFIER POTASSIUM CHANNEL TOK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="36"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="151"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="357"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="388"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="10">
        <location>
            <begin position="201"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="10">
        <location>
            <begin position="339"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ84283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="614" mass="70543" checksum="72F3AF7F8DD51A7B" modified="2017-01-18" version="1">MHSTISFEPWVVDQRTLQFNQRKINLLYIDARCKRFVFWFFLSCYGPVITSVMGPVANMLSVAALVNSWRQTPEGKEIKDRTGIYVINATSLGIGCISNIILFMHFSNRLTYITSQLWNIFGWSIAMILLVADLLWCWNKEIRFTDKQKGIGFWYAFFTVILYAGCTATLSLHFLGYKLKKYAPKFNLSEDERTVMMYTLWLSCWFMWGAGMFSQIMNISYGSAVYYCVVSTTTVGLGDVTPQTVAAKIMTLIFFLSSLLILGLIVTLTRTIVQKSFRPIVSLHISESKRSIFLKDLANKNDHRAHDYRDYNQFLLMRKTHKECESYAKRSGAILSVVIWILFWLIGALIFKFIEDWSYFNALYFCFLCLITIGYGDFAPSTGAGRSFFVVWALAAVPLMTCLINSVGEYLYDLGWSLSGNLNKAIRLTYRKINKAVSALGSHFFRNLELILTTTQRTNSGENTSSSDEQIKIELAENLSESDRSGDDSNLLSNKQLKIEKKIERLWLLAKDYSLLSETEPDHKLLYQEWVSIFSLLPDKQFEVIFSKNFWLSNVSPIRYNTNESRLAFLQLSSALKLEMKKLFNQDGNYNALGALNRQNSSDFLEEHNIQNEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDF0</accession>
    <name>A0A1E5RDF0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Spliceosomal protein DIB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2430</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 8">Essential role in pre-mRNA splicing. Also essential for entry into mitosis (G2/M progression) as well as for chromosome segregation during mitosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the DIM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDF0"/>
    <dbReference type="FunCoup" id="A0A1E5RDF0">
        <property type="interactions" value="932"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDF0"/>
    <dbReference type="InParanoid" id="A0A1E5RDF0"/>
    <dbReference type="OrthoDB" id="147752at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02954">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000004">
        <property type="entry name" value="Spliceosomal protein DIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004123">
        <property type="entry name" value="Dim1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12052:SF5">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12052">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEN 4A, 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02966">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017199">
        <property type="entry name" value="mRNA_splic_U5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01410">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4 8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00055473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00067627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR017199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ84932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="144" mass="17020" checksum="EDFFAEC4E5B16B2F" modified="2017-01-18" version="1">MGSVFLPHLHSGWHVDQAILHDQDRLVLVRFGRPQEKECLVMDEVLFEIAEKVRNFAAIYLCDIDEVPDFNDMYNLHDDPVTVMFFWRNKHMVCDFGTGENNKMDFAIDDEQEMIDIIETIYRGARKGRGAVNSPYDYNYKRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE34</accession>
    <name>A0A1E5RE34_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Suppressor of glycerol defect protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2362</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RE34"/>
    <dbReference type="FunCoup" id="A0A1E5RE34">
        <property type="interactions" value="653"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE34"/>
    <dbReference type="InParanoid" id="A0A1E5RE34"/>
    <dbReference type="OrthoDB" id="3973139at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050781">
        <property type="entry name" value="CWC22_splicing_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003891">
        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034:SF4">
        <property type="entry name" value="NUCLEOLAR MIF4G DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02847">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00544">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51366">
        <property type="entry name" value="MI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MI" evidence="5">
        <location>
            <begin position="891"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="16"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="422"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="17"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="65"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="192"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="224"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="284"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="345"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="422"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="455"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="477"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="497"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ85161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1151" mass="129955" checksum="4EF35BCBA6F75CB2" modified="2017-01-18" version="1">MSEENRHTIRIPGLILDELKNQESPDARFNQRDSKKRKYDGPRLGRKEKRKQERLAKKQKRTGKQSDFSSVTKQKYQDSLAKPQGVFSGSKNKNFRAQSAKAENSNGRAPKKSRDLPFSSDDDLDSDDFDDIDDELDSDEREQLRELEQEGSGSEDETGTSDNSENETDDDELDGTPEMTAAETMQALAALKENKRLASGLQRKDTALTADGIHSSSDHSDGSDYIDEEQDEINESDFDEVHESIDTENSLSVEETMAALKALKNKKNGDKTVRFATGTKAEANEEADSDTEIPADSYDLSADDFESEDESLGEDSEMTAEETMAALKAMKEKKKPLNNQDSTSKVKKIKHRSKKHGESDEEALEPILTPQEKALMERDEMDMQYYAKKLGLKGKTKKIKAKDEYDAIGGLLDGLDFFEDYGASDEDYGDLEMDKVNKADSSSEEESSESYSESESSDEDHDGDQEEDDTQATQFSSDDEIDSDDFDEFDENDLNEEEWKQLRELEGVASDESDVSEHEDSSSKKKKKKSKQKENPYVAPVSSVEEAASGAYIPPSLRKQQALATEDAKSLEIKKKIKIGLNKLSESNLANIVSSLSDLYSSYPRQLVSDIYADQMIELVSMNNKVLDSFVMNYSAVTFSLWKLKGVEMGATFVQKVVEEFLRNYHSDLAKYEEANAQDDSNDEKAPLFLSKKASNIVTLLAYCYNFGMISCKLIYDIINLLVSSPNEYTTEILLRIISVSGPMIRGDDPLALKNSLTNLLNNVKLLKNQTSRMKFLLDTVADLKNNRLKTSLLAPNHSSIKKSIVSALKLSSHGDPIQVSLFDIENVNTKGKWWLVGASWRGNDDSAFGALDNENGLDDMEIVDMEIEDDILDDIPDWGKIAKEQRMNTELRRSLFVSVMSAQDFMDAFTKIEKLNLKNKQSLEIPRVVLHCATVDDNGFNPFYSVLSSKLSEYNHNVVKGLQFQFWNVIKRFENDRSGYGVDTDGFEDDENDEETQQQVYGTDDGTRLKRISAQALFFGYLIADGTLKFDIFKHVPLIGGLNSDGVFFIELVLYQILKIVAKKSEAKKGKNVFEYQDNLLRQIFLKGIRAENVSMILKSLNWFVTKKFKPLKLIQHSKGSKEFLKEERRLSWAHKTLKKLIKGELEHLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE49</accession>
    <name>A0A1E5RE49_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Nucleoside transporter FUN26</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2237</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE49">
        <property type="interactions" value="276"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RE49"/>
    <dbReference type="OrthoDB" id="46396at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034257">
        <property type="term" value="F:nicotinamide riboside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002259">
        <property type="entry name" value="Eqnu_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332:SF88">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01733">
        <property type="entry name" value="Nucleoside_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016379">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="386"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="420"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="461"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ85182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="489" mass="54778" checksum="D59641807CCFCE82" modified="2017-01-18" version="1">MDASGNSTASTSAYEIKYVTFVLLGICMLWPWNAMLSLTSYMSHDVFKDTTIYAKIYTSTMMTVSTITSVFANYQLSKQQYGYSIRIKRGLIWQFFVFVVLAALVGLVRKFKLFLDFFIINLLVLISSVGTAYTQNGSMAWANVYGSEIYSQAIMMGQAIAGVLPSLVFFFLSFIKAGGDARDTSPVGIFFYYIATAAISVACYVLFKISKIGHEEKPVDQGNSASLSPAVNSAAYASHEEENDISSILRDTNNLNRKSNTESEDYTNKFIEDQNKQDPFSSAEQLEGFNTVDNAKRSLDEKASHIPLSVMYAKLKYLVLAIFFCFAATLLFPVFAANTYVSNFPLHNAQFIPLAFTVWNVGDLYGRYIASRYAFIFKNPXIVNPFNLFCYSIIRMIIIPFFFTCNIFGHMDSPFVLQDVWYLLLQFLFGVTNGNCISLSFMNIGDQLGDDDELKAASGGFSLIFVSSGLTFGSVLSYLFVYIINKIYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE66</accession>
    <name>A0A1E5RE66_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Chorismate mutase</fullName>
            <ecNumber evidence="4 15">5.4.99.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g2168</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the Claisen rearrangement of chorismate to prephenate. Acts at the first branch point in the aromatic amino acid pathway where it steers biosynthesis towards phenylalanine and tyrosine, and away from tryptophan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>chorismate = prephenate</text>
            <dbReference type="Rhea" id="RHEA:13897"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29934"/>
            <dbReference type="EC" id="5.4.99.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:13898"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; prephenate biosynthesis; prephenate from chorismate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.5" evidence="4 15"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RE66"/>
    <dbReference type="FunCoup" id="A0A1E5RE66">
        <property type="interactions" value="175"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE66"/>
    <dbReference type="InParanoid" id="A0A1E5RE66"/>
    <dbReference type="OrthoDB" id="191918at2759"/>
    <dbReference type="UniPathway" id="UPA00120">
        <property type="reaction ID" value="UER00203"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046417">
        <property type="term" value="P:chorismate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009094">
        <property type="term" value="P:L-phenylalanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.590.10:FF:000002">
        <property type="entry name" value="Chorismate mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.590.10">
        <property type="entry name" value="Chorismate mutase, AroQ class superfamily, eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036263">
        <property type="entry name" value="Chorismate_II_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008238">
        <property type="entry name" value="Chorismate_mutase_AroQ_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037039">
        <property type="entry name" value="CM_AroQ_sf_eucaryotic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002701">
        <property type="entry name" value="CM_II_prokaryot"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01802">
        <property type="entry name" value="CM_pl-yst"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21145">
        <property type="entry name" value="CHORISMATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21145:SF12">
        <property type="entry name" value="CHORISMATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01817">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017318">
        <property type="entry name" value="Chor_mut_AroQ_eu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48600">
        <property type="entry name" value="Chorismate mutase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51169">
        <property type="entry name" value="CHORISMATE_MUT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="9 15" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 15" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-0584">Phenylalanine biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0827">Tyrosine biosynthesis</keyword>
    <feature type="domain" description="Chorismate mutase" evidence="16">
        <location>
            <begin position="142"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR017318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ85185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="258" mass="30295" checksum="9E1B8D7891AF686B" modified="2017-01-18" version="1">MNFLEPSTVLDLQNIRDELVKMEDTIIFDFIERSHFARCSGVYQANNAEVDIPNFDGSFLDWSLMQIEIVHSQLRRFESPDQTPFFPNHILKPILPSINYPKVLASYDAEINYNTKIKNLYIDHIVPMIAKRSGEQKEQMGSVTMIDIETLQNLSRRIHFGKFVAEAKYQSNKELYKKLILEKDVNGIMENITNSQVEEKILERLRVKAQVYGVDPTNKEGERKITPEYLVRIYRDFVIPMTKEVEVEYLLRRLEDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE67</accession>
    <name>A0A1E5RE67_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pre-mRNA-splicing factor BRR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE67">
        <property type="interactions" value="113"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RE67"/>
    <dbReference type="OrthoDB" id="428895at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030532">
        <property type="term" value="C:small nuclear ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023251">
        <property type="entry name" value="Brr1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02039">
        <property type="entry name" value="SPLICEFRBRR1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="353" mass="40872" checksum="14E55F59AC0396EF" modified="2017-01-18" version="1">MEQKEILETHFGQNSVFDLTDKKVDPKVLEYLDSVRNEALTMTGIGAGFKDTESTKKQIMVETESSVKTEQSTVPDHIVDFLDRLPLIQLWYDESREELLNEDVEKSDRSEDENEHNVQAENKPPVGDYQTFKAVEPKKKLDYNAILLQKIDEYFQKQNYKPWIDRVQPLVQKANDTNSNQTLKTQLELDDKWAREFATWLLQQSFTSYEGIKQHLETFQDFQTKEPTSMGEWITYVERNLDKEPPFDIPVGTQFGLLSYVSKNHLNKDFSKVVTKILLTLPSKLLSTQISVLRDLGKLSALYLYNIESLHTSKTLRTIVIDDESSGXIPFNLAELVLTVLSEHYGQKDLIQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE80</accession>
    <name>A0A1E5RE80_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA replication licensing factor MCM5</fullName>
            <ecNumber evidence="12">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g1995</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the MCM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="12"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RE80"/>
    <dbReference type="FunCoup" id="A0A1E5RE80">
        <property type="interactions" value="1087"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE80"/>
    <dbReference type="InParanoid" id="A0A1E5RE80"/>
    <dbReference type="OrthoDB" id="10036721at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006267">
        <property type="term" value="P:pre-replicative complex assembly involved in nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17756">
        <property type="entry name" value="MCM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000241">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008048">
        <property type="entry name" value="MCM5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054125">
        <property type="entry name" value="MCM5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF42">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21933">
        <property type="entry name" value="MCM5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01661">
        <property type="entry name" value="MCMPROTEIN5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="14">
        <location>
            <begin position="434"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="108"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="387"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="648"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="109"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="388"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="649"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ85212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="859" mass="94695" checksum="D021AB98B240E0F1" modified="2017-01-18" version="1">MSSFDRPAIYSAPVLPGEELASNENTEIVKSFKKFILEFRLGSKFVYRDQLRNNLLVKQYFLKLNIDHLINYNEELFKKMYEEPVDVIPLFETGITEVAKRISYLISPNNSNNNNNNNDNNNNNNDNNNNNNNNNDNNDQNGGFETGMAISEDTITDFNNGITCQLILESGGHSTVTSATANANNDIVPIRQLNSSNVSKIVKINGIIVSSSLLASRASKLTLMCRNCKHTVRITLNNFNSISGNTSSSIPKSCLAEKSNANANNPAGFNNTGVNSAAQLTSASESCGNDPYLIIHEQSTFIDQQFLKIQELPESVPIGEMPRNILMTSDRYLTNKLVPGKRVSIIGIYSIYQSKNSQLNGNNNKAVAIRSPYLKILGISTTLSDTMGAGDSSDQQQQKSDGDGVGPQSGIVSSTNFNEQDEQLFLSMSRDPNIYERMSQSIAPSIYGNLDIKKAIICLLVGGSKKILPDGMRLRGDINVLLLGDPGTAKSQLLKFVEKAAPISVYTSGKGSSAAGLTASVQRDPTTRDFYLEGGAMVLADGGVVCIDEFDKMRDEDRVAIHEAMEQQTISVAKAGITTVLNSRTSVLAAANPIYGRYDDMKSPGENIDFQTTILSRFDMIFIVKDEHNEQRDISIANHVMNIHTGRGNNNNHNNDPNNIAGNNQNENSDFEEVPIETLKKYVSYCRAKCAPRLSQPASERLSSHFVNIRKQLLINELEAREKSSIPITIRQLEAIIRITESLAKLELSPVATEAHVNEAIRLFQASTMDAASQDPVSASGSGAGANSSSSYQDILRIEQELKRRLPIGWSTSYSTLRREFVDSRGYAQSALDKALYVLERQETIQMRHQRQNIYRAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REH0</accession>
    <name>A0A1E5REH0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2335</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M24B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REH0"/>
    <dbReference type="STRING" id="56408.A0A1E5REH0"/>
    <dbReference type="InParanoid" id="A0A1E5REH0"/>
    <dbReference type="OrthoDB" id="10261878at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052433">
        <property type="entry name" value="X-Pro_dipept-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226:SF1">
        <property type="entry name" value="XAA-PRO DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="7">
        <location>
            <begin position="38"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ84953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="158" mass="17940" checksum="48302150D2E08198" modified="2017-01-18" version="1">MKSRLKRLEKFGSSNQHPAAPRSREKSMTRFIIFDVNGLDTHDVGGEPNYSDPDPFLSYLRLRKPLAAGMVITNEPGIYFNDFLIKNFLEKHPERLEVVDVTVMEKYKYIGGVRIEDDVLVTKDGYENLTGITSDPDEIEQIVSNGIAKGRSHFHNIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REQ0</accession>
    <name>A0A1E5REQ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Postreplication repair E3 ubiquitin-protein ligase RAD18</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Postreplication repair E3 ubiquitin-protein ligase rad18</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16 18">RING-type E3 ubiquitin transferase RAD18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">AWRI3579_g1705</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RAD18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REQ0">
        <property type="interactions" value="313"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REQ0"/>
    <dbReference type="InParanoid" id="A0A1E5REQ0"/>
    <dbReference type="OrthoDB" id="9049620at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097505">
        <property type="term" value="C:Rad6-Rad18 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006301">
        <property type="term" value="P:DNA damage tolerance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000172">
        <property type="entry name" value="E3 ubiquitin-protein ligase RAD18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039577">
        <property type="entry name" value="Rad18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006642">
        <property type="entry name" value="Rad18_UBZ4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14134">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RAD18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14134:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RAD18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51908">
        <property type="entry name" value="ZF_UBZ4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 20" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14 20" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="22">
        <location>
            <begin position="38"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="UBZ4-type" evidence="24">
        <location>
            <begin position="179"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="SAP" evidence="23">
        <location>
            <begin position="344"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="228"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="276"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="242"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00074353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00082369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="OEJ85381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="474" mass="53858" checksum="58C6488500208E91" modified="2017-01-18" version="1">MSKKKTDLEYQLEKVTDSSDFASSSIPSLSDFDSLLRCHICKDFLSVPVVTPCSHTFCSLCIRQHLHTEAKCPLCLQDVQESSLVSEYLMSEIIKCYKKDLRGKLLTKLKTTSFAEPKEVVLAEASKMQERKINKLDHKENGLLLKHAPPKKKLKTDNKPKSIMSMLANRKTQSENLKLGQCPICSNLFPIKILQTTHIDQCLNGQTNHLSKESSAKPTKSHFIVDIDDEEDGEGTTSLAQKDLKGPKRHSMKYDSSVEIHDDETDLKVIATAETSISESEEAKVESAHHVPKXTNEDRILSSSEEASEKTEQPDLQTPFLNAYLDSAFAPSNAEKQRIPKLDTSNLALSXLKSKLMEWKLPTSGTKSQMVNRYNHWEMLWNSNFVDSLDPISEKELRSQLHVWEKHNNIDKSETTSKKDILVMFGSNNTAKIDLKSERFSRKNWSIINNQHYRRLAKQAKDSMNKKKVSLVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RET0</accession>
    <name>A0A1E5RET0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glucose N-acetyltransferase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RET0"/>
    <dbReference type="FunCoup" id="A0A1E5RET0">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RET0"/>
    <dbReference type="InParanoid" id="A0A1E5RET0"/>
    <dbReference type="OrthoDB" id="2014201at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050587">
        <property type="entry name" value="GNT1/Glycosyltrans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11183">
        <property type="entry name" value="GLYCOGENIN SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="492" mass="57367" checksum="B8B8727E229E7BA4" modified="2017-01-18" version="1">MILGRKKLRLTLVFITAVVCVFTFVEVLSTFQLNKEIEHYKYIMKKKKNNPGFFDPINIKQIPYAAIENLHSKRVNENKDSNGDVLDWNKFAYVNYVADAEYLCNTNVMFKKLLDSGTKAKLVLLVTSDLLEEPNSDIDVEAKLQDLKELSENQVLIKQIEPIYKPKDGTEWKNSLSKLLVFNETLYDRIVYMDNDALLRGETTNLDELFFLPSYIDFAAPLTYWMFTSSKIAHAYHEVANVEKWSTRLDKYIDESFLSAKKEDAYQFYNGLPNLPPSLYLSESKNIASEILESTSTISLSSTVKSLYATKNKNDVAKFASDLMVIKPSQELFDTIYTDLIPRNLKRKEKYDMDVINEEMYDLKKIIYYQFKLFKKLKKAFVPNVLVLPAREYGLLSGSLRNPKHYDFLKHDCIGYYDGIEKDAKNEKIEDIVAKWNKYTHFSDYPMGKPWYYDKSDDFECEIKDNLEDTEGACKTWQHDFRAFISEYEAVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RET1</accession>
    <name>A0A1E5RET1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mitochondrial phosphate carrier protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2455</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RET1"/>
    <dbReference type="FunCoup" id="A0A1E5RET1">
        <property type="interactions" value="757"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RET1"/>
    <dbReference type="InParanoid" id="A0A1E5RET1"/>
    <dbReference type="OrthoDB" id="427452at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990547">
        <property type="term" value="P:mitochondrial phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000024">
        <property type="entry name" value="MIR1p Mitochondrial phosphate carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044677">
        <property type="entry name" value="SLC25A3/Pic2/Mir1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671:SF12">
        <property type="entry name" value="MITOCHONDRIAL PHOSPHATE CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 (MITOCHONDRIAL CARRIER PHOSPHATE CARRIER), MEMBER 3, LIKE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="17"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="116"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="214"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ85412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="313" mass="32878" checksum="A275A8FDB61FEE8E" modified="2017-01-18" version="1">MSIAAADPVPTYNFSGMDYMKFFAAGAFCTGITHGALTPVDVVKTRIQLDPKKYSNGMFGSLKTIVSEEGAGALLTGLGPTLAGYSIQGACKFGGYEIFKKLAIDTFGYENAVAYKNSVYIGSAALAEFVADVFLCPLEATRIRLVSQPTFAPGLVGGFSRIFKEEGFGSFYSGFTPILFKQIPYNISKFLVFERAAEFYYGFTGPKETLSSTMNTLVTLGSGLTAGFAAAIVSQPADTLLSKVNKTKKAPGQSTLGLLGQLAKELGFFGSFAGLPARLVMVGTLTSLQFGIYGSVKSAIGCAPSVQIGGGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REU2</accession>
    <name>A0A1E5REU2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">54S ribosomal protein L16, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2147</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REU2"/>
    <dbReference type="FunCoup" id="A0A1E5REU2">
        <property type="interactions" value="305"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REU2"/>
    <dbReference type="InParanoid" id="A0A1E5REU2"/>
    <dbReference type="OrthoDB" id="268521at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="entry name" value="Ribosomal protein L10e/L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047873">
        <property type="entry name" value="Ribosomal_uL16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000114">
        <property type="entry name" value="Ribosomal_uL16_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020798">
        <property type="entry name" value="Ribosomal_uL16_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_uL16_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_uL16_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01164">
        <property type="entry name" value="rplP_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12220">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12220:SF13">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL16M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00060">
        <property type="entry name" value="RIBOSOMALL16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="Ribosomal protein L16p/L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00701">
        <property type="entry name" value="RIBOSOMAL_L16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="250" mass="28106" checksum="C1BF0B7379873593" modified="2017-01-18" version="1">MLCGCPNIKTLNFLETPLKNDQEQSTSEAIRLLTVMLNSFNHLRSSLVSSLSSLTFKRFRYTAKFGPSLVKAHKGRVPVRIGGSTKGSTVVYGDYGIRLATEGTRITQAQLKEADNILTRYVRKVGGDTRLYPRLVVNIPVFVKGNETRMGKGKGDFDHWMCRVPTGRVLFELKGTNLHEKMAREAFRKVGCILPGVYDFVMKGSLPRVGLKAFKDTSKIAKVNYLSQSNDRSKIKELSMSPEYSLYKNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REV1</accession>
    <name>A0A1E5REV1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Translocase of outer membrane 40 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g1737</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Channel-forming protein essential for import of protein precursors into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tom40 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REV1">
        <property type="interactions" value="820"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REV1"/>
    <dbReference type="InParanoid" id="A0A1E5REV1"/>
    <dbReference type="OrthoDB" id="19656at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07305">
        <property type="entry name" value="Porin3_Tom40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.10:FF:000009">
        <property type="entry name" value="Mitochondrial import receptor subunit TOM40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="entry name" value="Porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027246">
        <property type="entry name" value="Porin_Euk/Tom40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037930">
        <property type="entry name" value="Tom40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10802">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01459">
        <property type="entry name" value="Porin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="9" id="KW-0626">Porin</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00053390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00078731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ85431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="386" mass="41901" checksum="1A08390C89248851" modified="2017-01-18" version="1">MSSPASHLPVGDFSKLSALPKVQPLAPEQTFWNTNPLFSYLNDVYNNIHSHRKTLSLVNPGSIENLNKEVTRDVFLTQYAFTGLRADLNKAFCLNPVFQTSHTFSIGSQSLPNYAFSAMFVNDNVFAQGNVDNDFSVSGRVNYGWDKNNTSKLTLQMSEGQPTMCQLEHDFQGSDFSLNFKALNPFLGAKNTFTGVAVGSILQSITPNFSLGLETIYSRGQASAPADAAVSYVTRYVSSKKDWIASAQLQANGAVIGSFWRKVAPNVEAGLETQLQASVVPINDPVIGAPIGYQPVIEGVTTLGAKYEYRSSSFRGTVDSNGKVACFVERKILPTLGVLFSGEIDHFKNESKVGAGLQFETAGDEQILMMQQGLDPNGNPLQAXVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF62</accession>
    <name>A0A1E5RF62_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Acyl-protein thioesterase 1</fullName>
            <ecNumber evidence="2">3.1.2.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Palmitoyl-protein hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1753</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Hydrolyzes fatty acids from S-acylated cysteine residues in proteins with a strong preference for palmitoylated G-alpha proteins over other acyl substrates. Mediates the deacylation of G-alpha proteins such as GPA1 in vivo, but has weak or no activity toward palmitoylated Ras proteins. Has weak lysophospholipase activity in vitro; however such activity may not exist in vivo.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>S-hexadecanoyl-L-cysteinyl-[protein] + H2O = L-cysteinyl-[protein] + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="3.1.2.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. AB hydrolase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.22" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF62"/>
    <dbReference type="FunCoup" id="A0A1E5RF62">
        <property type="interactions" value="468"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF62"/>
    <dbReference type="InParanoid" id="A0A1E5RF62"/>
    <dbReference type="OrthoDB" id="2418081at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050565">
        <property type="entry name" value="LYPA1-2/EST-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003140">
        <property type="entry name" value="PLipase/COase/thioEstase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655:SF17">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02230">
        <property type="entry name" value="Abhydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0719">Serine esterase</keyword>
    <feature type="domain" description="Phospholipase/carboxylesterase/thioesterase" evidence="10">
        <location>
            <begin position="25"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="268" mass="29947" checksum="F68B485F14C37370" modified="2017-01-18" version="1">MVNNQNQNPPPTTKNSTPMQSYKIIKPLANPVKNCLIFSHGLGDTNEGWYAMFHQLTRQFPQVFQNTIVYLPLAPVQPVSLNFGMPMTSWFDIVSLSGSGNSTGIDDEAINARNFSQTEYVQSLKAITDLCMQISREHAIPTEKIVVGGFSQGAALSLGLSFYAPFKLGGIVALSGFPVMVDAKFIKKNHIDNLIINKDTPVFQGHGEEDPMVDLKFAENAKIFYTDENYLGNISKDKYMFKKYKHMGHSTCDEEMADMVDFLKTLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFB9</accession>
    <name>A0A1E5RFB9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1749</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RFB9"/>
    <dbReference type="OrthoDB" id="4067584at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="163"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="286"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="370"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="458" mass="53547" checksum="390F968996EE9687" modified="2017-01-18" version="1">MMDLQEKEHSRIPKLTNGYRHRLKRETENVFEPQTSTFKRRHTITSPSSTRTIATTSTETVDDAPLMNEITYGPLRRRRATMLALQNENNFRESALLETSLPRYSSLTRTSPSSSFATIRSPFRQNNVLLEKGSFQRRPTMRYNDPAVLERISDRDKKIMSELNQVHRAHTKASRELLALKEEVIPNLKYEISKKMTIMDKLTRDLKEIDDAISKVKKANTETQINNDEKLKNLQLEQKVSITNLRNEHFEDQQAHEKLLRQDFEEYKTAKMKEIKDLKCQDSKELQTLKLNNQTLXKDIEIKKQDENYKLEALQHEFEVQLKALSETNGSEIDGLKELQQXEYSSKLADIKAKIALKRESINDVKKPRIQELEFKISQLKANLQMLNSNKDINLKLQKLISEEYRPRVSNVENLELSLSKLKIKKDFLKKTLQKEQNHTGNLQDCINKLKSSRKSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFD2</accession>
    <name>A0A1E5RFD2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GPI inositol-deacylase</fullName>
            <ecNumber evidence="10">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g2394</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in inositol deacylation of GPI-anchored proteins which plays important roles in the quality control and ER-associated degradation of GPI-anchored proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the GPI inositol-deacylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="10"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFD2">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFD2"/>
    <dbReference type="InParanoid" id="A0A1E5RFD2"/>
    <dbReference type="OrthoDB" id="348976at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050185">
        <property type="term" value="F:phosphatidylinositol deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006505">
        <property type="term" value="P:GPI anchor metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012908">
        <property type="entry name" value="PGAP1-ab_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039529">
        <property type="entry name" value="PGAP1/BST1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056824">
        <property type="entry name" value="PGAP1_TMD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15495:SF7">
        <property type="entry name" value="GPI INOSITOL-DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15495">
        <property type="entry name" value="NEGATIVE REGULATOR OF VESICLE FORMATION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07819">
        <property type="entry name" value="PGAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25141">
        <property type="entry name" value="PGAP1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25140">
        <property type="entry name" value="PGAP1_TMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="757"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="799"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="855"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="967"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1000"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1028"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="domain" description="GPI inositol-deacylase PGAP1-like alpha/beta" evidence="11">
        <location>
            <begin position="189"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="GPI inositol-deacylase transmembrane" evidence="12">
        <location>
            <begin position="761"/>
            <end position="1065"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ85620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1068" mass="122293" checksum="FE2171C5AE23F2FD" modified="2017-01-18" version="1">MESRSCHVXRPPKNEGQFVKEQKRLNSRKRIKNTRSNIFKGSMGLRRSLNALFRLPRLWSAKNHSTPSKLSTNSRSVKDLNVLEKTASGGQPHYYSNDDDQKNYKRGNTSYYVKCVIGLCIVLLIALVSYKTPFKDSDRPQCRPIYMLPSYAKIDGFDDTHSRFSPKYHLYLYREQELDTLPLLDEEIQLDGIPVLFIPGNAGSFKQARSIAAAAAELYYNHPDQISNDPDSVKSLDVFTADFNEDFTAFHGRTMLDQAEYLNDAVRYILSLYASSKKTKDTGYGPLPSSVILLGHSMGGVVARVMPTLANYVPESVNTIITLSSPHSLSPVTFDGDILKIYDVTNKFWFQQYQNNSFYKNNVSIISITGGILDDVLPADYTNIEPIISNXQHSPNYENGFTVYTSTIPGVWTSIDHLAIVWCDQLRKVMAKILLEILDARVPSKTKSLPSRMKTFREFLLSGFESTCSQDLSLLAPDISTTEILDDQDLEPATRLKMDKNDLGKPLNYVFNISQENEVFNFLTSITDKXFYDILVCKNNPDYDGIKSASFSACVSLLSDLKNIPNSNFQSLEQSSIMNRNGHAPFQFLSLNTSLTKNYSRVVFQSKHPFDKDAFFIADVDTAITETVNFKAVDLMFQSKILQTSKDRFVSNYQFPNLWSSLLSYKLMVTQTGSANPLFNPLLKQYVAQPFETKWHILGKVAGKYRINTHNVLPYVLYPKNGDKALKLQLIKPFGVDLTLKISVDWEYTLKLLLLRYRLAIVALPTFLISLLFLWQICQFNRTGKFVSPKNALKEMLQHYFFHLLAAATILTPLCSLNAVKLMLQFLDPVKLEKFYDSNDYDSSFYFLGTNDWQMFWLGPMFILITFCLIFLASSFIDFVEYLXFKVSLVLNKHXXGAKDSFVRNFSFLQXXSLAFLLVLVLFFIPYQLVYVIITIMQVHVCMKFSKYSNDTDYENIRNYNKSLLQLFLFVVPVNAPTIIVYLHNFAIKWNTPFRSHHNLFAIFPXIFLVYCNYNGHLVSCHNKTRKFVLICALSFMTVFSLLYGVRYSYFIHHALNFVCCGLLLNTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFG4</accession>
    <name>A0A1E5RFG4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glucose-induced degradation protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2172</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GID4/VID24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFG4">
        <property type="interactions" value="358"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFG4"/>
    <dbReference type="InParanoid" id="A0A1E5RFG4"/>
    <dbReference type="OrthoDB" id="62at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034657">
        <property type="term" value="C:GID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045721">
        <property type="term" value="P:negative regulation of gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007039">
        <property type="term" value="P:protein catabolic process in the vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018618">
        <property type="entry name" value="GID4/10-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14534:SF3">
        <property type="entry name" value="GID COMPLEX SUBUNIT 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14534">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09783">
        <property type="entry name" value="Vac_ImportDeg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00061469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="390" mass="44680" checksum="F04C2C8729BA78B5" modified="2017-01-18" version="1">MVVNSIVTSAITTKYNGNDSLLQQVLKTQHELRHLHNNNDNDYTATNDNDNNNDNEDNGYLTQTGILTPKSSFLNLKNVLNENDKDXDKEEILDNLNNTIXLKNVNIIDDNNDNKLYDKQNRYYIDTDTSLEDIDSDIDSDTITDTITDTITNTITNTITDTITDTITNTITNTITDTIKDTITSSNSYQSFLKTNTVLQGCQMSRNEKYQVKINILHANTKTGELEGILSIYGLTNKYKKISTFFQGYCVDVNDEINQHEKRKSVXDECMKESSDHETNAFYYTKFNEIFGIDLFASXSKXHKRKNKNNXLLKKYTVMKWKERNLYLDFEQKTIEGASFQGFYYVVVDNENGCVKGFYHHPGSKDTGQQLSLXLERDMEXGNGSSFEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFH5</accession>
    <name>A0A1E5RFH5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">40S ribosomal protein S28</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1840</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFH5"/>
    <dbReference type="SMR" id="A0A1E5RFH5"/>
    <dbReference type="FunCoup" id="A0A1E5RFH5">
        <property type="interactions" value="828"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFH5"/>
    <dbReference type="InParanoid" id="A0A1E5RFH5"/>
    <dbReference type="OrthoDB" id="10258930at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04457">
        <property type="entry name" value="S1_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000025">
        <property type="entry name" value="40S ribosomal protein S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00292">
        <property type="entry name" value="Ribosomal_eS28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000289">
        <property type="entry name" value="Ribosomal_eS28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028626">
        <property type="entry name" value="Ribosomal_eS28_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01200">
        <property type="entry name" value="Ribosomal_S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00961">
        <property type="entry name" value="RIBOSOMAL_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="67" mass="7551" checksum="ABF4032F8F18A641" modified="2017-01-18" version="1">MDSKTPVTLAKVIKVLGRTGSRGGVTQVRVEFLDDTSRTIVRNVKGPVRENDILVLMESEREARRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RG95</accession>
    <name>A0A1E5RG95_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1838</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RG95"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="298" mass="34347" checksum="457E84CE4259B41F" modified="2017-01-18" version="1">MPSKEVXWKSYEIKNGLGHDWLMKVLLNLKARESEMLFTAFDQQAAQFYCFEISYENIDKSLKSLHVLSSKEQICEIFSQIYGYIGNDRLLVTSSSDFCFKICFLESHQEVEVSISIVDELNMIFRVPCNLQKESLHLYKELLSASGKYLQNXQYIINQLVDLVSDKIEVLXECRDMLVESGKQSFLDQLQKTNAQAFDNFHYKNWALEVMAKQSYEQEKAEPRRESPVLELDXFNLKQSPPKKRSXLKVYSGSTASKRKXDIAEFVNPENTKKILMPSLKASDDFSSASTSPKKKTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGD7</accession>
    <name>A0A1E5RGD7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribosomal RNA-processing protein 43</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1527</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ85982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGD7"/>
    <dbReference type="FunCoup" id="A0A1E5RGD7">
        <property type="interactions" value="255"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGD7"/>
    <dbReference type="InParanoid" id="A0A1E5RGD7"/>
    <dbReference type="OrthoDB" id="45882at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034473">
        <property type="term" value="P:U1 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034476">
        <property type="term" value="P:U5 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050590">
        <property type="entry name" value="Exosome_comp_Rrp42_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097:SF9">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="10">
        <location>
            <begin position="73"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="370" mass="40976" checksum="43C9CF96E122FD0A" modified="2017-01-18" version="1">MTAKDATDIQPVTFPPEVLARIAPDISLQRHLSLGLRPSLRKYEEFREYHSTPLTNDCNENPTGAPILGGNISKIGNMFVITTITGGIVEELVPVSGELEDLSNSNQSTDSGYAPVWPQVEVERGRVGAPTDEEMIVSEKLYETILSSQVIPNASLEVDVGVGYQDGTISYTDETMKPKNRKWTYVLYAKQQVFSRSGPIFDLLWNSLMLALQNVKLPRVYIDERATDLKIPIRTRGRSASIRETYDLMFDSQTLVDLKIINDNVSFASNFGVIALDPSLEIKVDEEEDQDEDMDKTLDTALAAKKPVLLADLQDDTEEGAVLSKMSISATADGTYKSLYLASADTRIPLVHLQTALKLSVQRSKDLSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGH5</accession>
    <name>A0A1E5RGH5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Glycerol uptake/efflux facilitator protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2323</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGH5"/>
    <dbReference type="STRING" id="56408.A0A1E5RGH5"/>
    <dbReference type="InParanoid" id="A0A1E5RGH5"/>
    <dbReference type="OrthoDB" id="3222at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015254">
        <property type="term" value="F:glycerol channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050363">
        <property type="entry name" value="MIP/Aquaporin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43829">
        <property type="entry name" value="AQUAPORIN OR AQUAGLYCEROPORIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43829:SF9">
        <property type="entry name" value="AQUAPORIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="386"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="436"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="522"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="574"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="131"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="676"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="729"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="29"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="52"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="140"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="181"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="201"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="223"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="680"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="695"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="756"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="773"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="791"/>
            <end position="803"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="828" mass="91554" checksum="A8EA689C4084ECD3" modified="2017-01-18" version="1">MSSSNHNNDVQSTFSRRTSNTGRQSHITSHGNNNSNAAASIHSGNTTTTGLNSIHTNHSHSANNNNNSSASNKGSSSGTTRRRRRRANSSFQYAHPQGMYQNPYMQQPQRPSPIQIEVIPNTDFAVNEAFHTPQAPYSSRRNDFGGSPRDEAHSDTHTLGSNANRASTTQKSGQSNRGNNDGDEYDEDLENNDPGLDDIPSSRSGRQSRSNNNNNDDNHNSDSEYEGLDDLDISDEDYDYRDPDHNQGRTNPQDPRSYNGSTFQQQGPGGDPNAPIVVVKPKQIHQNPQTPTVLPRGYAPINHWARFKHSRMKEFLAEFLGTFILIVIGDGVCCQVKLSEQHSINTFDKAMQLLAGRNAISNNNGTNATTSEIVSLLTKLVVPNGAGNFTVIMFAWATAVVLGFFASGGSAISGGHLNPAVTLTNFFFRGFPANKLFAYISGQLLGGYVGGLVVFGLYNRVIKEEFSDWKTNQTVIGMFCTVPLDYLRPKDQVVSELIATMTMQLGIFSLTDPYNNSSSDLFPVYLFFLIMACNASFAFQTGAAMNFGRDFGPRAALATVGVNKDALFNTNHHYFWVPIVVPIVGALLGATIYDAMIFQGHESPINTPWYQKVEFFKYWNRKILKYLTFGRYGRRKRSRRNRRIAFGSDSDDDDSSVNSETSNKFFDYDSANGSINELGSTNSSNSQSSSNASDDPLHDTEVNSSTHDLDHLPEDYPLADIDEVDEEQFNHNRSKRKSVGPFGDLDNGQETGEVTRGAKNEDSDGHDIAPATSRISFKANSRNPTTLAKNAKQKHQSHHSGKPSKREIHFVSSNKRHHRTPVPTVEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGI5</accession>
    <name>A0A1E5RGI5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Plasma membrane iron permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2062</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxidase-dependent Fe transporter (OFeT) (TC 9.A.10.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGI5"/>
    <dbReference type="FunCoup" id="A0A1E5RGI5">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGI5"/>
    <dbReference type="InParanoid" id="A0A1E5RGI5"/>
    <dbReference type="OrthoDB" id="4364at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033573">
        <property type="term" value="C:high-affinity iron permease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004923">
        <property type="entry name" value="FTR1/Fip1/EfeU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31632">
        <property type="entry name" value="IRON TRANSPORTER FTH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31632:SF2">
        <property type="entry name" value="PLASMA MEMBRANE IRON PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03239">
        <property type="entry name" value="FTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0410">Iron transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="150"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="296"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="397"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="420" mass="46859" checksum="64E2D4C291B75F37" modified="2017-01-18" version="1">MANKVFNVAVFFVVFREALEAIIVISVLLSFIKQAIGADIVLYKKLRLQIWLGSLAGFIICLIIGGAFIGAYYGLQKDVFGSTEDIWEGIFCMIATIMISIMGVAMLRLNKMQAKWRVKIAQSLVEKPKINEKTGKAKKWQGFSYLMKKYCMFILPFITVLREGLEAVVFVAGAGVTTTGSKASAFPLPVVIGLLCGGLVGAFIFYGGSYSSLQVFLVISTCILYLIAAGLFSRGAWFFENYRFNKASGGDASEGGDGPGSYNILKTVYHINCCNPELDNGWDVFNSLLGWQNTGYVSSMVCYNVYWICCIITVALMGFEEKKGHLPFCKNLKLRQVNPFYWIKGKNKKELNDEQKNEIFRKLHNVKFDQDGNMIQEEDNDNERVVSDENVADLKVSSNSNSDESLNDHKQGEKVVQTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGM3</accession>
    <name>A0A1E5RGM3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1362</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RGM3"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="176" mass="20491" checksum="DB220FCD45346A18" modified="2017-01-18" version="1">MEYLSKNTKVMKTRNNKLSGLYSIIGLGLFIYGYQKTNSEDQFYKQISSNTNSFLPLFQNSLYWNKYRHISYKEKHSLKSVYSNKLNNTLANATRNIKDQTRLALFKPKTIYVDTNNQISKPQQTIPKHGNXSKLGFEHEFEAKTLVKRIGIGLRSKVISKVRPEVRSAQFYNLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGP6</accession>
    <name>A0A1E5RGP6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Folic acid synthesis protein FOL1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g1560</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(7,8-dihydropterin-6-yl)methyl diphosphate + 4-aminobenzoate = 7,8-dihydropteroate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19949"/>
            <dbReference type="ChEBI" id="CHEBI:17836"/>
            <dbReference type="ChEBI" id="CHEBI:17839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="EC" id="2.5.1.15"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>6-hydroxymethyl-7,8-dihydropterin + ATP = (7,8-dihydropterin-6-yl)methyl diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">In the C-terminal section; belongs to the DHPS family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">In the N-terminal section; belongs to the DHNA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGP6"/>
    <dbReference type="FunCoup" id="A0A1E5RGP6">
        <property type="interactions" value="239"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGP6"/>
    <dbReference type="InParanoid" id="A0A1E5RGP6"/>
    <dbReference type="OrthoDB" id="615426at2759"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00155"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005740">
        <property type="term" value="C:mitochondrial envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003848">
        <property type="term" value="F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004150">
        <property type="term" value="F:dihydroneopterin aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004156">
        <property type="term" value="F:dihydropteroate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00483">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.560">
        <property type="entry name" value="7,8-Dihydro-6-hydroxymethylpterin-pyrophosphokinase HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="entry name" value="Dihydropteroate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045031">
        <property type="entry name" value="DHP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006390">
        <property type="entry name" value="DHP_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006157">
        <property type="entry name" value="FolB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000550">
        <property type="entry name" value="Hppk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035907">
        <property type="entry name" value="Hppk_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01496">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00526">
        <property type="entry name" value="folB_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01498">
        <property type="entry name" value="folK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941">
        <property type="entry name" value="FOLATE SYNTHESIS PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941:SF1">
        <property type="entry name" value="FOLIC ACID SYNTHESIS PROTEIN FOL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02152">
        <property type="entry name" value="FolB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01288">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00905">
        <property type="entry name" value="FolB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55083">
        <property type="entry name" value="6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="Dihydropteroate synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00794">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pterin-binding" evidence="16">
        <location>
            <begin position="501"/>
            <end position="786"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00009951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ86071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="792" mass="89382" checksum="523474EC06B61661" modified="2017-01-18" version="1">MPKTSLLKTGLQLPIRQKSTWRDRVYVQNLRVPKVVVGLNSWNVVQPQDVNVTLKMDTDFSKASENDDLNYSLNYAVISRDIGQKLSSKNFESLIEMGSFLSQHLLTQYQGIESMALTCDLLQGDIRCKNISVEISKDRDSEERLLLLRNIEVLTLIGVFTFERLQKQYVTLDIAVPLNTIESQSIDYRLIIKDVWSYIEQTNFKTVESLVERTSQLLIQKHKLDTVTVKVIKLNAITDTDGVGVSCVRNAKDFSGMSPVVAQLKEEETLQAHSNYETANNATFNLPVSVVKRENYNDSIAYVAFGSNMSYKLENIVVGLEELANVDGNVLLDCSSMFESEPMYFEDQDLFYNGIIKLQTNLPPHELLKLCKKIEYEILQRVKKFDNGPRTIDLDIVMYLDNKNENVVLNTPDLIIPHPRMLERGFVMAPLCEVIPSNMKHPISTEPLLDHLEQLKESPFGLGLHKILPLKNNSSIKFNLPACSSSKSDVTITKQKHSFPMCPMYVVNVTPDSFSDGNQDLINYDEKICEISGQVEKALNIYPEMEKIIVDIGGCSTRPNSVQPSGEEELLRVAPLLELLNKDTELRSKIVVSLDTYRPQVARKMINYIDIINDIGGDTLGKSSSGEDSLWKVVKDHPQVGYVLSHIRGDINTMSKLNIYSEDKNGECPKGMIEIIASELDNRLKECLKFGVKRWQIILDPGLGFAKVGTQNIQVLQNLHLFKQKFPNYPILLGPSRKRFLGAITNELVAANRDVETMALVSKLALDSDCDIVRIHSLTDCVKTLKIIDALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGX8</accession>
    <name>A0A1E5RGX8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1310</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the thioredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGX8"/>
    <dbReference type="FunCoup" id="A0A1E5RGX8">
        <property type="interactions" value="609"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGX8"/>
    <dbReference type="InParanoid" id="A0A1E5RGX8"/>
    <dbReference type="OrthoDB" id="10263751at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000245">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46115">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000077">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="30"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="33"/>
        </location>
    </feature>
    <feature type="site" description="Deprotonates C-terminal active site Cys" evidence="3">
        <location>
            <position position="24"/>
        </location>
    </feature>
    <feature type="site" description="Contributes to redox potential value" evidence="3">
        <location>
            <position position="31"/>
        </location>
    </feature>
    <feature type="site" description="Contributes to redox potential value" evidence="3">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="4">
        <location>
            <begin position="30"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ86116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="103" mass="11058" checksum="9E970EC3C3DC17D9" modified="2017-01-18" version="1">MVTQITSVEQFDQAIKADKLVVVDFYSLTCGPCKMIAPIVEKFAAQYTEADFYKVDCDDLGAAAQSNNISALPTFVFFKGGKEITRVIGANPSGVKSTIAANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGZ9</accession>
    <name>A0A1E5RGZ9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uridine permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1430</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGZ9"/>
    <dbReference type="FunCoup" id="A0A1E5RGZ9">
        <property type="interactions" value="569"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RGZ9"/>
    <dbReference type="OrthoDB" id="2018619at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11482">
        <property type="entry name" value="SLC-NCS1sbd_NRT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4160.10:FF:000001">
        <property type="entry name" value="Uracil permease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012681">
        <property type="entry name" value="NCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045225">
        <property type="entry name" value="Uracil/uridine/allantoin_perm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00800">
        <property type="entry name" value="ncs1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618">
        <property type="entry name" value="NCS1 FAMILY PURINE/PYRIMIDINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618:SF5">
        <property type="entry name" value="URIDINE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="399"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="440"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="464"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="517"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="555"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="628"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="641"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="654" mass="74059" checksum="B2565CE88968E71F" modified="2017-01-18" version="1">MVHPTTSNVSEVESAFDPFHHAHISHIDDKIETDEKDSSLGYTNSAIEENASPMDDFGKPADTKWNRLLTFLEVKQNHVAGHSGARSEDVMESFLFNDDLKPVEEARRVWDWKNYVFFWISGSFNVNTWQISATGLQMGLSWGLSWVTIWIGYISVAVFVCLGSRIGNFYHISFPISSRISFGIYFSLWVVLNRVFMAVIWFSTMVYIMGQTIQLMLIAIFGTDLPVRIKNTIGDTQPITTFEFMCFMIAWGVTLPFMWVPVHNLKYLFLVKSVIVPFAAFGFLIWTLVKFDGKVAVGSLSSFHPTGSLLGWNFIRSVMSAMDNFSTLILNAPDFSRFGKTAKSSVYSQLFILPLMYAIIAIIGIITTASAYTEYHENYWSPLDVLTRFLDHQTAGNKAGVFLIALAFCIAQLGTNIASNSISAGTDMTALLPKFINIRRGSYICAVISLAICPWNLMTSSSRFTTVLAAYATFLSSIAGVVSSDYFLVRKGYVRLLHCYTNKPGSLYMYGKYGTNWRAVVAYILALGLNFPGFIAEVSTAEVKVNENGRKIFYLNYYVGYLASFLFYFVLCYFWPIEGTRPNTKITDFKAFREVWVEVENFDEKRRIFLETGEEGEYDEYNDEYVFDESSENDNSSTSDEVNRIESEVVDLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH01</accession>
    <name>A0A1E5RH01_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">37S ribosomal protein S35, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1129</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH01">
        <property type="interactions" value="142"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH01"/>
    <dbReference type="InParanoid" id="A0A1E5RH01"/>
    <dbReference type="OrthoDB" id="10052321at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021036">
        <property type="entry name" value="Ribosomal_mS45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28158">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S35, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28158:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12298">
        <property type="entry name" value="Bot1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ86189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="360" mass="40969" checksum="FAF115C22D22783C" modified="2017-01-18" version="1">MFSSSMSNCSSSSCSYQLQQVRNISKRIKYPTYKFKFLPKKRPMKSDTNLRYAMRQFLGPKNYKGEYIFNKYYQAPTNNVPKYIVPQYEKGMALRDPKTGQVLSTTKIDSRRLIPSAIQRGARTLTSEKELHYIVERATPDFYKNTDSNPLHAPFPLNPFCKTNSLIDGSLKNYIYESIVTNKADPSVVSQKLKIKVPRLEAIIKLMSIEKDWEKSNSISANLENYXSSLSKMFPLYNARNDGENLQEIPIPENTKYSRFMVLAESQPFGPLDAANVLGLAPAESTLKKLADMSQGKNTKNKQNSKGKIVIGTKYKGEKSMFKFIDSKVGHVGYRYGAGNRDSKKDRKIGFNEVGQMIYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH15</accession>
    <name>A0A1E5RH15_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Hexose transporter HXT14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1309</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH15"/>
    <dbReference type="InParanoid" id="A0A1E5RH15"/>
    <dbReference type="OrthoDB" id="5290825at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF50">
        <property type="entry name" value="HEXOSE TRANSPORTER HXT14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="191"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="229"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="396"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="427"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="463"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="491"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="95"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="528" mass="58321" checksum="ABB7138B58E550F4" modified="2017-01-18" version="1">MTLAKASTRLNSRSTTKIKNFEKRHIQKVAPDEAMLNKPNFFSLETLTSSYSFLPIQTPVPNENEKHGNFSGNSSVLSTATTKTDRKFCYHLGKKSFALSVCGLIYGWEIGTVGGIMSMPSFRQDFTRLAPINIGMMISGFYIGCCIGGLFLSILSTPCNFEKCIAIGLAIYLVGILLEFVDIGVASSFRWIQFMVARVIVGLSVGCNSVMAPILMIEISSVHAKSFVALFYHVMCTFGILLGSCCNAYCIYFIEGAMQWKVPIVLGLLYSNALLLALCLMAPESPTQFIQGLLTPRKDTGFIMRSLHEKSKSNVXFRMPQWHDFITGKPKLGVRLLIGCLIMIFQQCCGINYFFYYSTMIFPLSNPYLISALLTLLNFLASGAAMLIXKKFSHRXLLVAGSLGCCLAMFTYATIGTFVQSSAKTVSIVIVTCVFIVFFASSVGPVSFAVVNDLFPQSVKNTSLAVCSITNWTCAYLIALIAPSMSARYGFSYGYLFFGFTLVSVIFYYFAVPETKNKTSNEIDQIYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHK8</accession>
    <name>A0A1E5RHK8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">General amino acid permease AGP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1615</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHK8"/>
    <dbReference type="FunCoup" id="A0A1E5RHK8">
        <property type="interactions" value="272"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHK8"/>
    <dbReference type="OrthoDB" id="3970526at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF17">
        <property type="entry name" value="GENERAL AMINO ACID PERMEASE AGP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="381"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="427"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="452"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="505"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="536"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="10">
        <location>
            <begin position="97"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="588"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ86381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="608" mass="67212" checksum="BB95EBA6A8E61A1A" modified="2017-01-18" version="1">MSSAQQEKAATINDKSNVSIHMQESSSTSYVFDFDPGHETTPSKPLLNSPFVQNFVDSFKRAEPGKTGFEDLEYSSSQKSESLGKTTELKKRIKPRHVFLIQLGSGLGTGLLVGNGSALREAGPAGLLIGYIIVSSFIYCTYNAIAELGISYSQLSGSYNEFSSILIDRAFGFSVACVYALQWLTIMPLELTTSSMIIKYWTTKVNPDVFVVIFYVILVIVNFMGGAGFAEFEFFMNSAKVCMLFGFVIFGIISDCGGSYGNGYTYIGGRNWKEPIGAFFGESKADHFKGICSSFVYAAFAYGGFETSILSAAEQVNPRKSIMKAGKMLLFRIIVMYCLFVLIIGLLVPHNSSRLLGNGSSASSASPLVIATSNITALPHIINAVILMSVLSVANASLFISSRVVLSICEQFFPRVPLLTYVDRRGRPLFGLILAAIIGLLSFVAASPSESIVFTWLLSISGLSALFTYLSMNLSHIRFRKAMKVQGKSLNEMGYLSKTGIWGSYYSSIIIALIFIAQFWIALVPVGTYKPDANNFFQNYLAMVVFVVLYFGFKIITKEWHIFIPSDQVDLSRGRKIFDHELLKQEEQEEAEKSKKKPLSMRIKNFWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHQ7</accession>
    <name>A0A1E5RHQ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">tRNA-splicing endonuclease subunit SEN15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1425</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEN15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHQ7">
        <property type="interactions" value="84"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHQ7"/>
    <dbReference type="OrthoDB" id="10002170at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018593">
        <property type="entry name" value="tRNA-endonuc_su_Sen15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09631">
        <property type="entry name" value="Sen15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="tRNA-intron endonuclease catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA-splicing endonuclease subunit Sen15" evidence="3">
        <location>
            <begin position="15"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ86113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="119" mass="14081" checksum="BFE1FB32F266FB0C" modified="2017-01-18" version="1">MEAHTLDRHGLVXRNVYMNMVHYLKWDDVQLMEFFDQESXEETKIILLTGINTQNDQAENAXREYIYPVHIXSXNVNNTLLKRIFRKISPKNWIHLGILSDDGTVVYYKLYNGLQKPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI02</accession>
    <name>A0A1E5RI02_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Bud site selection protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1533</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RI02">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI02"/>
    <dbReference type="InParanoid" id="A0A1E5RI02"/>
    <dbReference type="OrthoDB" id="546434at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF354">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="5">
        <location>
            <begin position="808"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="4">
        <location>
            <begin position="1071"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="193"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="35"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="196"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ86520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1362" mass="155147" checksum="5B2E26EF31D98036" modified="2017-01-18" version="1">MNRNQRELSNQSNGTFESSVDSFVTANSFPTDTDAFTHVRDLPKPSFEDESDKTPIIVQGEFQNDTTVSRNGTITARAGRNMEETTPTSNGGVIGTGIDLSPLPQHHNLPPRSPSPVRSLHTFEQPLFRKPRITSSYYQPLEEARQSLMQEHHAEPVETESLLEQDDLAFNETTMDSINTNVVSPDRVINEEHFAQNISPPTSIETTKNGGKGEPASNDEEDLPTSTGHEILNNSQLDLDTEEYTNLFIVATKAFDKNVLEEEDDKQVCLSFEVDDVAFVHAIEESGWGEVTIVKVNKRGWVPLNFFTDAIKPTSTFLPKNFLHLSSLESKQIIQLRAQILSREPLLAFFSACGRFLVSPQRVPTETANEYTFDIEDINRIKAGIKKLLEKTGCISRTNALVQAKALVRKTRKRLLADWYSVALKADSCKGTTSSEKIQKLEEMIFQVIRRAFLFYSIWGIEVQAYENEKRLIKLKDRKSFLPSLAAQERPASIDLPQDVKCLDQPPMAISRLNEINNYLFTYIGLVLGRMDIIEHNPAGCEALEYVVYQIILLLRELLYISKACSTLISSKYQNSYENNLDNNLDPLLSLVSELVSAVKIFVTKTLQETATRTNSLRHSMIKFENQSYSHTAEASDFIRVLSQMTGFISNAISSCNNYLRIIGDFQLGDEKEYSDFKSSSIAPEKFVKICSTSILDEIKRLDLKAVTGSPETADKSFARFSTIRAGEGSLSNQLRLTNEGGEFLRECEAADYNDEGLAENDQKSFLRNSVFEQFTVPDENLFDQQEREALLIMDREAMQNELVYDKFTQDLLGGSFRALVFHLTDEVHKPDEFFTTSFLINFKSFGTSKELLTDLFARFDTENKSYKSGLNKKSEAFSSWASQLKHRRKLVVKVIKLWLESYWDHIADFSYLPTLMNFFNECVSNHLPIESRNMIEIIAKLMTVSPDSSHSQLISRSIKVANINSVLSKLSSISTNDSDQVNEDIVDDFDLASNAGDSLPVPVAGLGTSNLLSLTHAQECKKLVEKYRDLAFRTSERSSLDKLISTWFYNVKNNQRIQFSNHEIQFCDMNAMEVAKQLTLIEARLFAAIDPMELLNGRFLEKNKSLGQAPNVSRLITFTNALSNYVIESIVSPLFSLKIKQLRIRQWMKISLSCLYFKNFNSLASIMIALQSHVISRLKTVWEGFTTKDHKLLEFLMKIIHPNQNYKVYRKKLAIFVQQAKNMYMNNGDNNSALNGDKANSLIVPFFNLFLQDLTMINEGNNNYRNPESFRPNKIINIDKYFKVTKIVQKVQELQVFIIDNLDFEEYTDTRDSLYLIGKETIKSLPVLQEFVLYELWRVNLIYTNEPDRGYDMSLKMLPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RID1</accession>
    <name>A0A1E5RID1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Vacuolar protein sorting-associated protein 75</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1549</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the nucleosome assembly protein (NAP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RID1"/>
    <dbReference type="FunCoup" id="A0A1E5RID1">
        <property type="interactions" value="604"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RID1"/>
    <dbReference type="InParanoid" id="A0A1E5RID1"/>
    <dbReference type="OrthoDB" id="19419at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.90">
        <property type="entry name" value="Nucleosome assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037231">
        <property type="entry name" value="NAP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002164">
        <property type="entry name" value="NAP_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11875">
        <property type="entry name" value="TESTIS-SPECIFIC Y-ENCODED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143113">
        <property type="entry name" value="NAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="225"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="228"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="270" mass="31659" checksum="5D25C57E8ADD9C7F" modified="2017-01-18" version="1">MANEKLSLALQGLANIEDELEACDKKVQNYRLSVLQPVYNKRNALIQDVPDFWKIVLTQHQDFADYIQVEDFKYVENISSIEIDWESIDEFQITFNFQGIEDDFPKQKVCKKFKLEYDVDKVYNPNKLKPEELEDMEEFGYWTSEPCAIQWPASYNGFNPAKIMDKSSAIGKKNYRKGMKSFFGWFKWTGLKIGKEFPNGQDLATLFTDDIYPHCLKYYTQAHRDLEDERDGTDSEVDDEEEPAFEIPEEVDEEEEEEEQEPASKKVKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN88</accession>
    <name>A0A1E5RN88_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Histone deacetylase</fullName>
            <ecNumber evidence="3 10">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g853</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the histone deacetylase family. HD type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="3 10"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN88"/>
    <dbReference type="FunCoup" id="A0A1E5RN88">
        <property type="interactions" value="168"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN88"/>
    <dbReference type="ESTHER" id="9asco-a0a1e5rn88">
        <property type="family name" value="Arb2_domain"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RN88"/>
    <dbReference type="OrthoDB" id="424012at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031078">
        <property type="term" value="F:histone H3K14 deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019154">
        <property type="entry name" value="Arb2-like_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017321">
        <property type="entry name" value="Hist_deAcase_II_yeast"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF5">
        <property type="entry name" value="HISTONE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09757">
        <property type="entry name" value="Arb2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037919">
        <property type="entry name" value="HDAC_II_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0678">Repressor</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="12">
        <location>
            <begin position="155"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="Arb2-like" evidence="13">
        <location>
            <begin position="542"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="489"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="505"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF09757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ88350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="806" mass="90889" checksum="86ECC85C79B25150" modified="2017-01-18" version="1">MKSETNLDTHQSVQEASGDESNGIRMQSIHNNRNNNRKRYLELVPVVRPVKLRYVPLKTGVCFDVRMRYHSKLYTSYFEYIDPHPEDPRRIYRIYKILCENGLIDDPSLSIASNSEIEATEEPKNINGKESSSEINVKKENSFSTFDSNNTNSNGLGEYMVKIPIKEASIEEILLVHSKEHLAFIEKTEKMSRQDLLKETETGDSVYFNNESFLSARLSCGGAIEACKSVVEGKVKNALAIVRPPGHHAEPERPGGFCLFSNVAIAAKSILKNYSESVKKIMILDWDIHHGNGTQKSFYNSDQVLYVSLHRFELGKYYPGTKIGDLDQCGEDKGEGFNCNIPWPMGGVGDADYMLAFRKVIMPMAQEFKPDLVIISAGFDAAEGDTIGQCHVTPACYGQMTHMLKSLARGNLVVVLEGGYNLDSIAISALDVAKVLIGEPPNEIENIDPKTGEIVVPKPEAIKTVENVIKQQSKYWQCFKTPEGNQGLPLAFWAPPTPNNEQKKTASPPSETSASKNESKQNSPLSSDTPAISMLPPSIVPLNKSLDNYRFQYLQEMYTFYPLPLIHNPIISEIKAQMENFIIGTTFNPAQHLTDTQNLVVLIHDTPEMWGKPNPITGVLDPSQTYLIDTTLKIIEWCEKQKYAIIDITIPTNSQNTVFYSQEVMMYLWDNYLQYFNNMQKIAFIGIGDSTAGLVHLLGHRDTREYTKCCISFVDKTSLKSIVPLIDQSLVDWYYKNSLVFASKNHTCWSSNTKPRKKYGRVLKCLKDGMCDIFDEKFEEATDFILDSFEEFDDDDEDGEDEESST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNB3</accession>
    <name>A0A1E5RNB3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Maf-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g707</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNB3"/>
    <dbReference type="FunCoup" id="A0A1E5RNB3">
        <property type="interactions" value="118"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNB3"/>
    <dbReference type="InParanoid" id="A0A1E5RNB3"/>
    <dbReference type="OrthoDB" id="10267058at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047429">
        <property type="term" value="F:nucleoside triphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00528">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003697">
        <property type="entry name" value="Maf-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00172">
        <property type="entry name" value="maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213">
        <property type="entry name" value="BIFUNCTIONAL DTTP/UTP PYROPHOSPHATASE/METHYLTRANSFERASE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213:SF5">
        <property type="entry name" value="BIFUNCTIONAL DTTP_UTP PYROPHOSPHATASE_METHYLTRANSFERASE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02545">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006305">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="238" mass="27295" checksum="2E62CBAD7CA8AC44" modified="2017-01-18" version="1">MVHSHLITKIHSKYDVILGSSSPRRREIITKNIGFQNDKVKIMKPSFEENLNKGLYLEDLYRYAKDTVKHKAMSLIPDLEKEYCETKASLQQQQQQQQQHDEILVICCDTTLVDPVDGIIYEKPKDREDQLQNLQKFCYVLHELKCVSSVCIMLYCPSTKKYETHEFNSITSIIFDENIPKSILQDYVNSGEASDAAGGFKIQETAATFIKSIEGDFFNVMGFPVNKTVQELIKIIDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNC4</accession>
    <name>A0A1E5RNC4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">MICOS complex subunit MIC27</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g697</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNC4"/>
    <dbReference type="FunCoup" id="A0A1E5RNC4">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNC4"/>
    <dbReference type="InParanoid" id="A0A1E5RNC4"/>
    <dbReference type="OrthoDB" id="4039294at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ88381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="230" mass="26530" checksum="7AF1FF793DCE4A5F" modified="2017-01-18" version="1">MSTDSQLPRRRFYDVSSKEHVVISSEEASHTPIPVKGLTSEVSIYEPLLEKFHDLHQSVNHFYKTSVLEYQNLTHAAKNEIQNSKNYLNENFVVGNEYEMKEKLVPSVFYSLIGFYTGRIVTNPHNWNSVIFGTKSLLGKITTSLPSRLLFPIALGAYTFENQTPITYQRVGAVVKHDVLHKDTIEIIDIGKQEVEKTRKLFNDLSETIDLTLQKQIHRLRQYAVDTWPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNG1</accession>
    <name>A0A1E5RNG1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">6-phosphofructo-2-kinase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g830</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNG1"/>
    <dbReference type="FunCoup" id="A0A1E5RNG1">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNG1"/>
    <dbReference type="InParanoid" id="A0A1E5RNG1"/>
    <dbReference type="OrthoDB" id="267323at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003873">
        <property type="term" value="F:6-phosphofructo-2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000644">
        <property type="entry name" value="GpmB, Fructose-2,6-bisphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003094">
        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013079">
        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF32">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01591">
        <property type="entry name" value="6PF2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00991">
        <property type="entry name" value="6PFRUCTKNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="5">
        <location>
            <begin position="476"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="129"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="314"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="387"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="780"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1061"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="634"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1165"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="46"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="149"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="279"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="320"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="350"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="387"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="433"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="797"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1069"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1130"/>
            <end position="1142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ88427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1199" mass="137076" checksum="9331C7CC7A0FF9EA" modified="2017-01-18" version="1">MSFRKVDFHQPKKSSLKDDLYSTSPYTATNKFELPIKPLEEPALAPTQTVEESLPASASPQQQQQQQQQQAQTSGSPNTVIAPDPQRSDGQGVPSDGSSLSFQTNDITNSHNELFLQGKKSHNLQVPKISETKSAADLRTNSLEGYGSDVEEGAEEEAEDEQWDNHPLTQVETVKLEDIANDDKINVDDIFSEKSNQVCGDKLEQVNDKNNEQKNENVKYNAPNSAMNAFFNTNASAVPIQSTDNTSKKAALFHNLKHQGLLPTFQKRPLSDTPVHSLMNSPNTNNTPNLSPLPSNLEMSDIDERTNDLITNRQTNASANDNNNNNNYYNHNTYNHDNNKNNKFFGNGHKNNESTHYTDTDYTSSGMDDSEADSFYANNNTDNFNYTTGENATDASNYSTYKDLNDSSSSPNIMNNGYSPDNNIRNSSDRNSARRQRKSVARKHLKKPPLKKRASSVMTTLDVPGLTKSKISPDGHIAQQDAGAKLVIVMVGLPATGKSFLTNKLSRFLNFSMYECRVFNVGNTRREYASKHKDVVPEQDSKFFDPKNENSNHLREQWAMDTLDQLLDYLLDMHGNVAIFDATNTTKLRRNNVVNRIRDRSKHLKIVFLESICSDEELVAKNIKLKLLGPDYKGKNSQQSLEDFENRLKQYRKAYQPVEDEEVEKHNVSYVKMIDVGKKVISYNIQGYLASQTIYYLLNFNLSDRQIWITRNGESEYNVSGKIGGDSKLTTRGLKYSKALAQFIRKKRKEFNYEMRLKNLNVLKDMERRESNLQKELQEIEEGKRSALSPESQDHHQTHHHHSQSHNKSTSHLHHHHHHNHHHHNHHRDTDPSPDQQEENVEMSDGVPPSRPAHEFHLPNFFVWTSMLKRSIETAQFFDEDEFPIKEMKMLNELSAGDFEGLTYQEIQQEYPEEFEERLHDKLLYRYPGIGGESYFDVINRLRTVITELERITDNVLLICHRVVARVLLGYFLNLSKEIYSNLDVPLHSVYCLETTPYGVTWKLFEYDEALNDFHEIPQDEINTKVVQQVGIVHQERRFSVVPTLGRKETDLKKLLDSNSSKEKLTHLGESSSERTNLWKNSSRPAVLSTNPFTSARNVPRSSIMNSQNSNKSVLLRRNDSGNNTIKPQEQQKEHIPNKDDETVMNSPKDLTVPFSSAENIHSTRKQIMDTLETHQLEIEKLSEKLQNLKQNDKSDDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNK0</accession>
    <name>A0A1E5RNK0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Calcium channel YVC1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g680</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNK0"/>
    <dbReference type="FunCoup" id="A0A1E5RNK0">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNK0"/>
    <dbReference type="InParanoid" id="A0A1E5RNK0"/>
    <dbReference type="OrthoDB" id="301415at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056337">
        <property type="entry name" value="LHD_YVC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052971">
        <property type="entry name" value="TRP_calcium_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056336">
        <property type="entry name" value="YVC1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35859:SF5">
        <property type="entry name" value="ION TRANSPORT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35859">
        <property type="entry name" value="NONSELECTIVE CATION CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23190">
        <property type="entry name" value="LHD_TRPY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23317">
        <property type="entry name" value="YVC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="428"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="483"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="514"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="YVC1 N-terminal linker helical" evidence="2">
        <location>
            <begin position="24"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Calcium channel YVC1-like C-terminal transmembrane" evidence="3">
        <location>
            <begin position="256"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ88420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="650" mass="75009" checksum="B8BDF4A00F7C5E62" modified="2017-01-18" version="1">MREDHLLPISKKESLLPTPNSRQILRIAINLKELIDTVIPVEFDESLIVSNKSRIINTNVITLARQACGGDISNPSSISKYQAVLIFCLLKVRNWYLSLGDSQLHDYNLYVLRATACQQLCKYIIEEEEDSGEYDYLLINMLCRRYVINENDVDQEPLSALELASDTHALVVIGSSGFQRCQKFLWRGWIIQNRQDPMQYVLAHDFITNTKFWAHFNPERLKCPMYQNALQLFFSVIYLILYTIMVNSKNSSAVQPIDFIEGAFYTFTIGFIFEEMVKWYHVGSYYLNFWNIFNNSTYVLVIISMIVRCISINHLFNIHDPRAIDTLSYRILACASPMIWSRIIFFLDAEKFMGTMLIVVKQMMKESLIFFVLLIFIMIGFLQGLLALDSADGSSDITGPIVGNLLLTILGESSFKMFEEFAYPYSAILYYSYCFIIAVILLNILIALYSSAYQNIVDNATDEYLALMVQKTLRYMRAPDENVYIAPLNLIELLLQPLAMMMKRLNKNDYMVKVMHHHVMTMLYFPVLIVVSIKEVKEAKRATYNRLKRLPPDANELDTPWDLEDGFVDEDADSVFTSNIRKGLKATNAKNRNTMQKQQLAEQEDPYFSVDQAWHENVKNSSQPVDKGCDSGVGWENYAIYEKVCSLKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNK1</accession>
    <name>A0A1E5RNK1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-histidine N-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.85</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g825</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the methyltransferase superfamily. METTL18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.85" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNK1"/>
    <dbReference type="FunCoup" id="A0A1E5RNK1">
        <property type="interactions" value="1588"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNK1"/>
    <dbReference type="InParanoid" id="A0A1E5RNK1"/>
    <dbReference type="OrthoDB" id="1723750at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018064">
        <property type="term" value="F:protein-L-histidine N-tele-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF39">
        <property type="entry name" value="HISTIDINE PROTEIN METHYLTRANSFERASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 10" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ88456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="410" mass="46034" checksum="9122F4A1CEB8130F" modified="2017-01-18" version="1">MVVMSCRAKHVTTSSIKETDKIHKFTLIVLNGPNMSFKFDFQDDDIDAEIDQQQISNHSDNVVASAANPLDEIESTIVQTGTIIAPEQVDLQALLASLKDVRFTFDIFQTPIEQVTLYRREVFDVKHQLMQEENSFSSAELEILMGDTNEDLKQFIYEGGLKSWECSIDLVDYLTSQNVQAAIPETVVEIGCGTSLPSEHLFVEYLKKFCTPNLEASCAPGLKLVLTDYNKSVLRLVTVPNLVIAWAKTVYGSHWSDESVWGAELQSGFPTQEDELTLTQELIDNFNTFCSIYKIEICLVSGSWCRSFQNILHSLLTKHGENDDSTLVISSETIYQPQTLPVITETLIDLQNSFRQLPNGKQAILVAAKDIYFGVGGSIIDFTNYLDQKQSQYEVIKVKAGLKRSIVIMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNU6</accession>
    <name>A0A1E5RNU6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g789</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNU6"/>
    <dbReference type="InParanoid" id="A0A1E5RNU6"/>
    <dbReference type="OrthoDB" id="5364312at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="356"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="403"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="492"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="284"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="356"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="403"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="430"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="445"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="502"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="518"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="545" mass="58121" checksum="1F9FFE2C78449C50" modified="2017-01-18" version="1">MSAINSYSTNPRGSNTSTFEKTAGLSRSTTNHSFQHSVASNGESVLIDESDLSDNEHGENDHNNNNNNNNNEDFDHPFGMAPASKVPSNAASMIFERTVEDPFVPINPHNNLTRTNSIVSLGNGRTNSIVSLHNLSRTNSNANVGNLFSSSISSTYNNNPINPINNNNNNNNINPNNLSRTNSWADMRAGSSPADINGRRSSMSIHRTLENYIAPSLDASAHLVGDKSTNLDQIQIQHLQANGMYNNHPNRRPSTLGLYTTSGAATPIPTTSEQRSAGNTQDESMSSTSPISPLMSPSSPNLVSSSYVSSSSTSASSNNGRPKILRMYSYADVLNDEKQFRRPSLSQSSSSSILLNTNSFNSSNSNNNNNVPATTSNTPASLNTSNSAIGQGASFSIGSPLSSSFEQNSGNSYNSNNSNNSSLVSPSFSKPISTGSNNGSHPLASNVINSDSIKINNNNNNNTTGNSVRHGSSVGKDASPLSKRAIMTHHAVNKTGGLPNDGTPSRRTSQSSGSGVFEKLKPEEYKQRQYGVDQGGKSPLHYIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNW9</accession>
    <name>A0A1E5RNW9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative aldehyde dehydrogenase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g910</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNW9"/>
    <dbReference type="FunCoup" id="A0A1E5RNW9">
        <property type="interactions" value="307"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNW9"/>
    <dbReference type="InParanoid" id="A0A1E5RNW9"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006598">
        <property type="term" value="P:polyamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000001">
        <property type="entry name" value="Aldehyde dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000012">
        <property type="entry name" value="Betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43720">
        <property type="entry name" value="2-AMINOMUCONIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43720:SF2">
        <property type="entry name" value="2-AMINOMUCONIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="6">
        <location>
            <begin position="26"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="504" mass="54940" checksum="A8EB362184C886B9" modified="2017-01-18" version="1">MLFKEITLPTGTKYEQPLGLFINNEFVESHSSERLPAYNPATGKVIVEVQEACAKKDVDLAVEVATKAFKSWKKVPAVEKRDMFFKLAQLVSENKQLLAEIESANSGKPVETNAKGDIDEMIDVFKYLGGWIDKVDGETHIPDDKRLCLTFHQPMGVVGCIVPFNYPLSMMTNKFAAIAAGNTTIFKSGDQTPISALFFATLVKKAGFPPGVFNLLSGKGSTVGDRIIKHPGIVKVAFTGSTAVGKMIQENTAKNLKPLSLECGGKSPAVVFDDCDFEQAIKWCALGIFFNTGQICSGTSKVYVQDTIYEKFIESMKKYVEENYIVGDPSVESTVVGPLISAKQHDRVTGYIKKSIEEGLHLVTGGLETPASVASNETLKQGYFVQPTIFRDLQPHNTIAKEEIFGPVLAIAKFSTYDEVIELCNDVDYGLGSAIFTTNLQKSIAFAKDIEAGIVWVNSSNDANSNCPFGGIKESGNGSKDLGSYTMLNYMNVKSVQINLSSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP00</accession>
    <name>A0A1E5RP00_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD(P)H-hydrate epimerase</fullName>
            <ecNumber evidence="3">5.1.99.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g616</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-NADHX = (6S)-NADHX</text>
            <dbReference type="Rhea" id="RHEA:32215"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:64075"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(6R)-NADPHX = (6S)-NADPHX</text>
            <dbReference type="Rhea" id="RHEA:32227"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:64077"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.99.6" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP00"/>
    <dbReference type="FunCoup" id="A0A1E5RP00">
        <property type="interactions" value="268"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP00"/>
    <dbReference type="InParanoid" id="A0A1E5RP00"/>
    <dbReference type="OrthoDB" id="10064708at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052856">
        <property type="term" value="F:NAD(P)HX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10260">
        <property type="entry name" value="YjeF N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004443">
        <property type="entry name" value="YjeF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036652">
        <property type="entry name" value="YjeF_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032976">
        <property type="entry name" value="YJEFN_prot_NAXE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13232">
        <property type="entry name" value="NAD(P)H-HYDRATE EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13232:SF10">
        <property type="entry name" value="NAD(P)H-HYDRATE EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03853">
        <property type="entry name" value="YjeF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64153">
        <property type="entry name" value="YjeF N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51385">
        <property type="entry name" value="YJEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjeF N-terminal" evidence="11">
        <location>
            <begin position="8"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="47"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="53"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ88615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="318" mass="34818" checksum="D95CDCD923A3EDB6" modified="2017-01-18" version="1">MLLNSIKAAKIDAELMSSKYGFTLQQLMELAGFSVAQAINKEYGHFDGKRGASSGTSSGTSSGTSSGTSSGTSSDTSSDTSIGESSGICNNNNNSGSRKDILVIVGPGNNGGDGLVAARHLALFGWTPVVYYPTYEKSINKQPFYLELCEQLESMFQVEIVKKLLSSQNKPNFPQSPQLSFFSKFPFILDCVFGFSFKPPIKSGSTFESVLQDMITLQKTIVNHPIGKSHKIVSVDIPSGWHVDQGPIENEPFLKPSMLISLTYPKQCCKFLPESTVHYCGGRFISDAFAKEYGFEPIPYKDDLQVYKFPSKWAGQRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP06</accession>
    <name>A0A1E5RP06_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Serine/threonine-protein phosphatase 4 regulatory subunit 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g615</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP06"/>
    <dbReference type="FunCoup" id="A0A1E5RP06">
        <property type="interactions" value="933"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP06"/>
    <dbReference type="InParanoid" id="A0A1E5RP06"/>
    <dbReference type="OrthoDB" id="27483at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030289">
        <property type="term" value="C:protein phosphatase 4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072542">
        <property type="term" value="F:protein phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055236">
        <property type="entry name" value="EVH1_PP4R3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006887">
        <property type="entry name" value="P4R3-like_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051137">
        <property type="entry name" value="PP4R3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23318">
        <property type="entry name" value="ATP SYNTHASE GAMMA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23318:SF0">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22972">
        <property type="entry name" value="EVH1_PP4R3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04802">
        <property type="entry name" value="PP4R3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PP4R3 EVH1-like" evidence="5">
        <location>
            <begin position="176"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Serine/threonine-protein phosphatase 4 regulatory subunit 3-like central" evidence="4">
        <location>
            <begin position="290"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Serine/threonine-protein phosphatase 4 regulatory subunit 3-like central" evidence="4">
        <location>
            <begin position="438"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="567"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="746"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="811"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1068"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="567"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="751"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="815"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1068"/>
            <end position="1079"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ88614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1088" mass="123528" checksum="E89E6D1FF87C5781" modified="2017-01-18" version="1">MASTVDRPCGNDDTKTFNRLQNKNNDNVNGIHDNQNNRENHSTLNYSTNNTNIKNTELIQDFDIDEMHDSSFSQSSSPITPETDQIDVDLHHLECVTQQKRVKVYLLENNEWKDIGTGFCTGEKNYDETTEIGCDKITENNHENNENSQTKNNIKNNIKNNIKNITNNIKNNTQIEPFLMVREEKSLENILLISKLEGNVEYQRQEDTLIVWKDLNGRDIALSFEESSGCDHICEYIVNVQLNVVKSISLVAIKSDLITNTTVHEIIAGPVNLPSTSFHLSIIELIESLKIFNENLTYQFLKTETINFIIDQKYFTCLIKNFEIAEFEKNWKKLLLLSSILKTIILFNEQIILDFILKDEYFQGMLKILEYDSEYPTMKANYKETFSEIIKEQQTKNQIWFEMNKWDQKMGQMGNLSKNSDTKLVLLNTTKDEDTQLEECRMVVNQLCKLTFLRDIVLVRYLEDYPIITDCIQEDQTFVIDYMTNKSSILDQIVDVYLNSLDKNQKLDSLKLLHDCVMMAKTSLVDHSEKAEFFIKLVHAGLFKMLQNAFGLNTSATEATKTSSSFFSSSSSSSSSSSSPSFASTNSEHKDATEIDENIKLLATDLIVIIIEHDVLLVNSNSSIASAAAAAADIIASEDNQKITTESVESSETSFSNTNTESNQIATKAIGTTVKHTTHTTDTGLEESQHDMSLLSIISNLLVYGTGPCLKDQLLQTLLTLLHPEGNTGAEDFLTNNYSHFEIATAANKNDDDDDDDDDDDDVNDVESDYHMENNKPTGDGDMLQENFEVAETNDKVTTTSTIYTKAKVEKNDLDDMENSDEDENDADYEEGDNLNNEVNFSGMVIDEDMVESSEDETAANEHINVFLNKFYELIAPGLFKPLIDNKFSDYSSAIYLVKLVKFIAIIHTRDISRKFILQNSLMQNISKLAMDPNSTLQLKLASIRCLKCIISLNDEKYIDCVISENVLLSVFELLNENKFKDNLCTSTILDFVKNIMDLKQDTKSAKSIRNFVALKDYILINFHDVLANMNTEPLIQDFLSLDNRERGQLTSNSAECMDTDELVAERKHNEHKRLREDTPEPAASGFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV39</accession>
    <name>A0A1E5RV39_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Meiotic recombination protein DMC1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g402</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RecA family. DMC1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RV39"/>
    <dbReference type="FunCoup" id="A0A1E5RV39">
        <property type="interactions" value="186"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV39"/>
    <dbReference type="InParanoid" id="A0A1E5RV39"/>
    <dbReference type="OrthoDB" id="10251254at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070192">
        <property type="term" value="P:chromosome organization involved in meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000730">
        <property type="term" value="P:DNA recombinase assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042148">
        <property type="term" value="P:DNA strand invasion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000709">
        <property type="term" value="P:meiotic joint molecule formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000239">
        <property type="entry name" value="Meiotic recombination protein DMC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011940">
        <property type="entry name" value="Dmc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016467">
        <property type="entry name" value="DNA_recomb/repair_RecA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010995">
        <property type="entry name" value="DNA_repair_Rad51/TF_NusA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020587">
        <property type="entry name" value="RecA_monomer-monomer_interface"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003301">
        <property type="entry name" value="PRK04301.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02238">
        <property type="entry name" value="recomb_DMC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942:SF30">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN DMC1_LIM15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942">
        <property type="entry name" value="RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005856">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47794">
        <property type="entry name" value="Rad51 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50163">
        <property type="entry name" value="RECA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0469">Meiosis</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="10">
        <location>
            <begin position="87"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="RecA family profile 2" evidence="11">
        <location>
            <begin position="265"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ90802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="327" mass="35849" checksum="0C5E6BA432E8E783" modified="2017-01-18" version="1">MSDTSITTQDIVQIDELQNFGINAADISKLKVAGIYSVNTVLSTTRRNMNKIKGLSEVKVDKIKEAASKIISIGFIPATVQASKREKVIAISTGSKSLDNILGGGIMTMSITEVFGEFRCGKTQMAHTLCVAAQLPKEQGGAEGKVAYIDTEGTFRPERIKQIAVRFDLDPDVTLDNIMYARALNSEHQTELTDQLGNELVHGDYKLIVVDSIMANFRVDYCGRGELNERQQKLNQYLSKLCRISEEFNIAVFMTNQVQSDPGASALFAASAKPVGGHVLAHASATRILLRKGRGEERVAKLQDSPDMPERDCTYIISEKGICDPDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV93</accession>
    <name>A0A1E5RV93_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Anaphase-promoting complex subunit 4 long domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g383</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV93"/>
    <dbReference type="InParanoid" id="A0A1E5RV93"/>
    <dbReference type="OrthoDB" id="2110451at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024790">
        <property type="entry name" value="APC4_long_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12896">
        <property type="entry name" value="ANAPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 4 long" evidence="2">
        <location>
            <begin position="591"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="919"/>
            <end position="941"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ90759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="971" mass="111360" checksum="8EFD5100F3251AEF" modified="2017-01-18" version="1">MDQILRKSFQYIKQNHQELDHELIDLSANLQFVKTPQSIITNTGIPCVSELLNSMKLTTWSCDLSNEVKKSKAKKTDRASKDSFNDYIMHENILHPDLYFPLPINFSQKNISGNNHYLIIYKAVPVGSERIDASNSSDFIDELEQNDTGRTKNDESKETYVTGYIDNFSVSAINSKYHNKNMEFVKYATLPIPVGYGSKIIDYSWAPITKEEEQERIGSNHGMQNNTFIINSDQMKNETQSHQKKTQFSQSKPFLPVSSNANKQNIQLLIVTDTHFLFYDLDNGGKLICKGKSLHEGIDLLRETRNMPKLETINNDENVFRDELLDSMLIKHADLNDFKTDPHCALWYKLYKKKSALTLMSSDFGSQMPQLAYNFDIKSSTANKNDQNDNATGVPYTSLSLLKAGNHTHSTLYFLAMQAMHGPLGFSNTDTLIGKSGDIFKNENFLFSKKPPVLNDFTLKLHNLNQKQEENQTNVFSNSMGTPSSNTTNSNTVCLDHLNSFIFQNVIENGDEDALVLDREISLFLTVLDDGLIILDIDSNFQVPIYLNLSALQSNANDNFGQNEQHNYQVKIYDSSTLLLYDENFIVACNISHLFENYLLMNMVESFTKLESLIAYWKEVLNVSIVKSFIQPFIKFFKNLCSAYNNEESNNRLMEELVELFCTGNYTTELKDWFVFSLGSKNTKKWTKLFNEMYDISVQLTMLNSCAERLLLEVDTFLANAKSFVTINQLTSLPTFILYTQKIKQELSNNILIPLNLMLNGSWKVEYQQHLDFLKWLTMWCLHMEDEDKPIHIVHPEKINSIMHFMTEXLPNLDSKKSDCSSLVLLEFQRQNSXHIATVENCIKIMKSYTASFMGVLVAQDMDYFEPLNETYQINDVSIINNSIHVIQSSEAENSLQIYDMEHDYLVIDLIKDLQEECTEDDNGYPGSNSXPIKRSPKHAAGKRRIKKAQFSNNKCTNSLTNNFDVDVLYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVJ4</accession>
    <name>A0A1E5RVJ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Aspartic proteinase 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g335</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVJ4"/>
    <dbReference type="InParanoid" id="A0A1E5RVJ4"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05474">
        <property type="entry name" value="SAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033876">
        <property type="entry name" value="SAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF65">
        <property type="entry name" value="ASPARTIC-TYPE ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184932" evidence="11">
        <location>
            <begin position="23"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="12">
        <location>
            <begin position="73"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="661"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="144"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="661"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="478"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="513"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ90869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="737" mass="78807" checksum="8A4BF40BB418EAFA" modified="2017-01-18" version="1">MQTKSVARSLLVSALCFGYVQSAAIGDLEPKQVGVRYGIERGSKNVGSKSKFVKRDDGSDVLKFVIDNQQNYYSVNISVGTPPQQVSALLDTGSSDLWLLGNFDDVSACEAYLKAVGIEDSTSSSSSSKFENVVENDVVYSSFHRVLNKYEKKLEQEIQQEKQEQLQKKKQARAVAEKAITTGVRSSYTASGSATQTVDLESYFDTMSFETSMDSTNTADPFQSYDTAAPTETNFDYFSDYYGSYETATATTDAGSYASMSQDMEQAEASQVLEGCEDSGVYTPADSSSYKKNDTTAPFVIQYGDLTFASGWWVSDSIAINGIDVPLQFAVANVSNSSSVLGVSYESSEASIAYSESGDSQSSDMKKKKRXGNSQKELESFTYPNLPVALKNQGLIDKISYSLYLNSLEQPFGEILFGAVDSTKYTGQLYTIPLVNIYAERGVENPVQFDVTLQGTGISLGDGKTKTFSTTKLPALLDSGTTFLHLPVDLAEMFAESVDAEWDDDMESFVMTCPSTKDQNDISFAFDMGGFLVYTPLGNYILETTDXSTCVLGIYPSDELYVILGDNFLSSVYVVYDLEDYEVSIAQANFDGAAENPGKIEAISSAVPSAKKAPMYSSTWSTAMKISTGGNIFTDSSASNPWTATNNNGLSTVSLSASGITXTPSSSSTGSSTISSTGSSTGSPTGSPTGSPTGSSTGSSSSSSSSKSKNAGNAMNSFALSNENIFAISLLLLSSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVL7</accession>
    <name>A0A1E5RVL7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">N-terminal acetyltransferase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g529</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVL7">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RVL7"/>
    <dbReference type="OrthoDB" id="3971729at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045866">
        <property type="entry name" value="FAM210A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009688">
        <property type="entry name" value="FAM210A/B-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21377">
        <property type="entry name" value="PROTEIN FAM210B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21377:SF0">
        <property type="entry name" value="PROTEIN FAM210B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06916">
        <property type="entry name" value="FAM210A-B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1279" evidence="2">
        <location>
            <begin position="68"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ90866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="261" mass="29637" checksum="10FBD7B5EE46C492" modified="2017-01-18" version="1">MFRPGLHSIKRSLIARQFHNQTLYSAKQSFLPSRVSLFPKFKIRKNFYSSAVKSGGSASAAQPKKQGKLQELIRKYGVSALGVYLFLSCIDLPICFYFVHQLGELKIKLYINNAKRFVGFSEKSDQDIEQEVLKHQAEQEEQNKLQKEREESGLXNXASKKFSLQRYWDHLKNTTLLTEFLIAYGIHKSLIFIRLPITAAITPSVVRTLQRMGFKINTKAASPTPFINKGAASLGGANPANKYSLQSTKTNEKTKKWFGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVN2</accession>
    <name>A0A1E5RVN2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g341</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RVN2"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031464">
        <property type="term" value="C:Cul4A-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000109">
        <property type="term" value="C:nucleotide-excision repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042238">
        <property type="entry name" value="Rad28/ERCC8/Ckn1/ATCSA-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46202">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46202:SF1">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ90991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="431" mass="48949" checksum="9B250271DFA47C7F" modified="2017-01-18" version="1">MINDFQLYWDRPANKKESMLLQSGFNSLTIYKPRSKAARNSFNYDVQFPTSTMAIDSTCDWFLQGKKNGRIALWRFEDAKPSSSSSSVSAQENQEEVFLKSKKHTSVKTPMVSISSNGLLTKEANDPVYELKWYPKDNGMFYQVGENSVRIWDTAAMEPVFECPAIQSNVLGQRYKIDVCDDLILYGDQLIDLRTLSSHTQIKSDSKISSNSIFHEDVGVLRNGDATSESSNTCIFTGHLDGFMNTWDLRTLKKPLQRTKIGNGMINHITGQSVNQLESSNNWNSSLREKRESQINDIWVVNNXTTVTKVWENGTTVLSTFKCENIVGSKIEQLYVDQTDQIMYCRDSKKVYIYFYEQAGHANKAAANACDTNGKESASKTPEFMQDRQQISYKYWNQFKTPENSNITCMDISGGHHLFGLQNGGIAEWLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVP9</accession>
    <name>A0A1E5RVP9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Inositolphosphotransferase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g455</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVP9">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVP9"/>
    <dbReference type="InParanoid" id="A0A1E5RVP9"/>
    <dbReference type="OrthoDB" id="5784at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070916">
        <property type="term" value="C:inositol phosphoceramide synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006676">
        <property type="term" value="P:mannosyl diphosphorylinositol ceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03386">
        <property type="entry name" value="PAP2_Aur1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026841">
        <property type="entry name" value="Aur1/Ipt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052185">
        <property type="entry name" value="IPC_Synthase-Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310:SF8">
        <property type="entry name" value="INOSITOLPHOSPHOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14378">
        <property type="entry name" value="PAP2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="289"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="461"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="489"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Inositolphosphotransferase Aur1/Ipt1" evidence="7">
        <location>
            <begin position="168"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="400"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="400"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ90848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="561" mass="64192" checksum="7CB35658806C1BB7" modified="2017-01-18" version="1">MTILRTILKVPIVIVQFLIAVYFAGLHNKYNVITLPFNFFVNFLPIVIWLTIFKKAGEIPITWRPDIHGKTSFMADMFMFGDYWGEILVQYQDRAHGVLLHKLSFVVSTLGFALPFLVCLPLALWYYIYYVKKYTKNLVSYHLPLXNQNPKTQLHIVFIPLMVWMALNVDHQFASQAESNFKDWKNMMSWIFYVLAHLLAPIFTAVYLYVFKAPGTLKCFSLAMGLXNICGVFTHLLLPMAPPWFTHMYGLFNNTELNYNTPGYAAGLIRVNFKAGTHLASEGFHKSPIVFGAVPSLHSAMAFQCFLFIWFNRVQSAPPSALGVSLGNDIEESCTQNNDTTDLEHGVSKGLPVFAEAETQSAQSLQSLQSMQSETPKENFTVEEFDMEDMLLAEDTKSVXSDQHSRTSLSRESFAEDSPVATEDYTPDPALADLRSFNNTSTDLRSFKSKSRLVRVIKSGWIPRVIVSLFLLVQWWATMYLDHHYRFDLFVGTCYSMSSFLLINHFVLQKRVLTAFAEKREMMMNPSKENPXDDEGMSMGMRVFXGTKIEWVFDSFQRQPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVQ1</accession>
    <name>A0A1E5RVQ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">dihydrolipoyllysine-residue succinyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">2-oxoglutarate dehydrogenase complex component E2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g323</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.61" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVQ1"/>
    <dbReference type="FunCoup" id="A0A1E5RVQ1">
        <property type="interactions" value="874"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVQ1"/>
    <dbReference type="InParanoid" id="A0A1E5RVQ1"/>
    <dbReference type="OrthoDB" id="5391403at2759"/>
    <dbReference type="UniPathway" id="UPA00868">
        <property type="reaction ID" value="UER00840"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004149">
        <property type="term" value="F:dihydrolipoyllysine-residue succinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033512">
        <property type="term" value="P:L-lysine catabolic process to acetyl-CoA via saccharopine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000006">
        <property type="entry name" value="Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050537">
        <property type="entry name" value="2-oxoacid_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006255">
        <property type="entry name" value="SucB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004309">
        <property type="entry name" value="PRK05704.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01347">
        <property type="entry name" value="sucB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43416:SF5">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43416">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="14">
        <location>
            <begin position="57"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="132"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="158"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ90783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="446" mass="48696" checksum="4A7D9BA75677EFEE" modified="2017-01-18" version="1">MFSATTRTTSTRRLALLAKSSSPSSSIPAKRVLTNNNYNSSLNSNNAFSVYQKFYQSTVVKVPTMAESLTEGSLKEYSKQVGEFIKQDELLATIETDKIDIEVNSPIDGTISKLFFQPDDTVTVGDDLATIEAGSAPEGGSSSDAAATTTTTTPPPSPKKEDSKPEPKKEDSKPEVKKEESKPEPKKEESKPEPKKPEPKPAAPPVSSAPAQAFSRGEKAVKMNRMRLRIAERLKESQNTAASLTTFNEVDMSAVMQMRKLYKDEIIKTKNIKFGFMGLFSKACTLASKDIPAVNAMISGDQILYRDYSDISIAVATPKGLVTPVIRNAESLSVLEIEQEIVKLGKKARDGKLTLEDMAGGTFTISNGGVFGSLYGTPIINMPQTAVLGLHGVKERPVTVNGQIVSRPMMYLALTYDHRLLDGREAVTFLKTVKELIEDPRKMLLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVQ7</accession>
    <name>A0A1E5RVQ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anaphase-promoting complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g330</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the cullin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVQ7">
        <property type="interactions" value="658"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVQ7"/>
    <dbReference type="InParanoid" id="A0A1E5RVQ7"/>
    <dbReference type="OrthoDB" id="5581181at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007091">
        <property type="term" value="P:metaphase/anaphase transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044554">
        <property type="entry name" value="ANAPC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014786">
        <property type="entry name" value="ANAPC2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057975">
        <property type="entry name" value="TPR_ANAPC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45957">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45957:SF1">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08672">
        <property type="entry name" value="ANAPC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25773">
        <property type="entry name" value="TPR_ANAPC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01013">
        <property type="entry name" value="APC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cullin family profile" evidence="7">
        <location>
            <begin position="522"/>
            <end position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00016068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ90916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="855" mass="99194" checksum="AAE501E23F7C58F7" modified="2017-01-18" version="1">MNTILNQEIDASLNEIINLLGPYPSEAEKREQTDDVEMLLGWMDPIDQQHGFKPPPLRVKKIINSILETGKAFKYKEDVTSVFPKHRTSFFAQNNEFFGLPIYSSNFDQTPDDTGDGVSIVERHLLSIYFTRVRLHFFTNFQNVTSLKMAFKLEELYRFPKKFCFWRNEVLFENEIMILRKYLLNKNHVLELAVIDLLKKCNLQKDLVQVNLMYQWMSSVTEGRFLLIDLMLDTVKSLCCSVCKQPVPLSNTSIVDLVYKKFYFSFWVGHYENMLGVSNEEEKTELLNEIRLVVICEFVRVCAVQSYDLCIXDLSHSYSKLRELKPILMEHSQLCQLFAQSLSNQISEKLLQSSAPTVTLLYSYLQVFKALNFLDANSKTLKQTVLSKLVGTLRDRQDMTKIFLYALFNLLASDIALIDDSALKLDFETLGKIHHELSIRLNFANTSMMPGDTTLSHPLHHPPTSMMAWFEKENFEWQPEVLQETDKITNYNSFIKIVTKTMTHDVYPRVQQDGFDAVFYIIFNNKKTRILIETNEIMKKLLLVAKEESLKPEWRACLQILQNKIKNRVTGNEQSLLNNLNIMLHDFSQSIIMRQQGLRTSMLLPKVISYIYWEMVEDYQKSSQIELPSVMQKEFEKLSKLYSRKFQHGCKLKMYAGKGLVELKLSFDDGRVFEKDVHMYEALVINCFDVNDLKKTGKSGLSLEYIKNKLKCDEDLITKALEFWMSENILYGQNGFYHVIEDLTQYENLIKKGGKRVTSLGDGFDRMESPVRNEDNNQISQLVKSMEKVKPFLVGMLTNHGSMKVGKMFSFLNMTVPKDLGFKCTQSELATYLDILVQEKVLTVTLKGAYKISKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RW46</accession>
    <name>A0A1E5RW46_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Pentafunctional AROM polypeptide</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="23">3-dehydroquinate synthase</fullName>
                <shortName evidence="23">DHQS</shortName>
                <ecNumber evidence="23">4.2.3.4</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="23">3-phosphoshikimate 1-carboxyvinyltransferase</fullName>
                <ecNumber evidence="23">2.5.1.19</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="23">5-enolpyruvylshikimate-3-phosphate synthase</fullName>
                <shortName evidence="23">EPSP synthase</shortName>
                <shortName evidence="23">EPSPS</shortName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="23">Shikimate kinase</fullName>
                <shortName evidence="23">SK</shortName>
                <ecNumber evidence="23">2.7.1.71</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="23">3-dehydroquinate dehydratase</fullName>
                <shortName evidence="23">3-dehydroquinase</shortName>
                <ecNumber evidence="23">4.2.1.10</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="23">Shikimate dehydrogenase</fullName>
                <ecNumber evidence="23">1.1.1.25</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="31" type="ORF">AWRI3579_g367</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="32">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="22 23 24">The AROM polypeptide catalyzes 5 consecutive enzymatic reactions in prechorismate polyaromatic amino acid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23 24">
            <text>3-dehydroquinate = 3-dehydroshikimate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="EC" id="4.2.1.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21256"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="EC" id="2.5.1.19"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:21257"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23 24">
            <text>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate = 3-dehydroquinate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21968"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="EC" id="4.2.3.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21 23 24">
            <text>shikimate + ATP = 3-phosphoshikimate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13121"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.71"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23 24">
            <text>shikimate + NADP(+) = 3-dehydroshikimate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17737"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="23 24">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="23 24">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="23 24">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="23 24">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="23 24">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 23 24">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 23 24">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="23 24">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 23 24">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the EPSP synthase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="23 24">In the 2nd section; belongs to the EPSP synthase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the 2nd section; belongs to the type-I 3-dehydroquinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="23 24">In the 3rd section; belongs to the shikimate kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="23 24">In the 4th section; belongs to the type-I 3-dehydroquinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="23 24">In the C-terminal section; belongs to the shikimate dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="24">In the N-terminal section; belongs to the dehydroquinate synthase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the shikimate kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="23">In the N-terminal section; belongs to the sugar phosphate cyclases superfamily. Dehydroquinate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="31">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.4" evidence="23"/>
    <dbReference type="EC" id="2.5.1.19" evidence="23"/>
    <dbReference type="EC" id="2.7.1.71" evidence="23"/>
    <dbReference type="EC" id="4.2.1.10" evidence="23"/>
    <dbReference type="EC" id="1.1.1.25" evidence="23"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RW46">
        <property type="interactions" value="507"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RW46"/>
    <dbReference type="InParanoid" id="A0A1E5RW46"/>
    <dbReference type="OrthoDB" id="197068at2759"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00085"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003855">
        <property type="term" value="F:3-dehydroquinate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003856">
        <property type="term" value="F:3-dehydroquinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003866">
        <property type="term" value="F:3-phosphoshikimate 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004764">
        <property type="term" value="F:shikimate 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004765">
        <property type="term" value="F:shikimate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00502">
        <property type="entry name" value="DHQase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08195">
        <property type="entry name" value="DHQS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01556">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01065">
        <property type="entry name" value="NAD_bind_Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00464">
        <property type="entry name" value="SK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1090.10:FF:000007">
        <property type="entry name" value="Pentafunctional AROM polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000135">
        <property type="entry name" value="Pentafunctional AROM polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1970:FF:000007">
        <property type="entry name" value="Pentafunctional AROM polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001256">
        <property type="entry name" value="Pentafunctional AROM polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.65.10.10:FF:000007">
        <property type="entry name" value="Pentafunctional AROM polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="entry name" value="Enolpyruvate transferase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00210">
        <property type="entry name" value="EPSP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03143">
        <property type="entry name" value="Pentafunct_AroM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00109">
        <property type="entry name" value="Shikimate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018508">
        <property type="entry name" value="3-dehydroquinate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016037">
        <property type="entry name" value="DHQ_synth_AroB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030960">
        <property type="entry name" value="DHQS/DOIS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056179">
        <property type="entry name" value="DHQS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001381">
        <property type="entry name" value="DHquinase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006264">
        <property type="entry name" value="EPSP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023193">
        <property type="entry name" value="EPSP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008289">
        <property type="entry name" value="Pentafunct_AroM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041121">
        <property type="entry name" value="SDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031322">
        <property type="entry name" value="Shikimate/glucono_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013708">
        <property type="entry name" value="Shikimate_DH-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010110">
        <property type="entry name" value="Shikimate_DH_AroM-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000623">
        <property type="entry name" value="Shikimate_kinase/TSH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023000">
        <property type="entry name" value="Shikimate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01356">
        <property type="entry name" value="aroA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01357">
        <property type="entry name" value="aroB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01093">
        <property type="entry name" value="aroD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01809">
        <property type="entry name" value="Shik-DH-AROM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21090">
        <property type="entry name" value="AROM/DEHYDROQUINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21090:SF5">
        <property type="entry name" value="PENTAFUNCTIONAL AROM POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01761">
        <property type="entry name" value="DHQ_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24621">
        <property type="entry name" value="DHQS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01487">
        <property type="entry name" value="DHquinase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18317">
        <property type="entry name" value="SDH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08501">
        <property type="entry name" value="Shikimate_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01202">
        <property type="entry name" value="SKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000514">
        <property type="entry name" value="Pentafunct_AroM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01100">
        <property type="entry name" value="SHIKIMTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01028">
        <property type="entry name" value="DEHYDROQUINASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00104">
        <property type="entry name" value="EPSP_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00885">
        <property type="entry name" value="EPSP_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01128">
        <property type="entry name" value="SHIKIMATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 23 24" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="17 23 24" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="14 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 23 24" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 23 24" id="KW-0418">Kinase</keyword>
    <keyword evidence="18 23 24" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 23 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19 23" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="15 23" id="KW-0521">NADP</keyword>
    <keyword evidence="11 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16 23 24" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 23 24" id="KW-0808">Transferase</keyword>
    <keyword evidence="13 23 24" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="73"/>
            <end position="185"/>
        </location>
    </feature>
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        <location>
            <begin position="187"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="Enolpyruvate transferase" evidence="25">
        <location>
            <begin position="406"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="domain" description="Shikimate dehydrogenase substrate binding N-terminal" evidence="28">
        <location>
            <begin position="1304"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="domain" description="Quinate/shikimate 5-dehydrogenase/glutamyl-tRNA reductase" evidence="26">
        <location>
            <begin position="1423"/>
            <end position="1498"/>
        </location>
    </feature>
    <feature type="domain" description="SDH C-terminal" evidence="29">
        <location>
            <begin position="1551"/>
            <end position="1580"/>
        </location>
    </feature>
    <feature type="region of interest" description="3-dehydroquinate synthase" evidence="23">
        <location>
            <begin position="1"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Shikimate dehydrogenase" evidence="23">
        <location>
            <begin position="1299"/>
            <end position="1583"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for 3-dehydroquinate synthase activity" evidence="23">
        <location>
            <position position="262"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for 3-dehydroquinate synthase activity" evidence="23">
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            <position position="277"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for 3-dehydroquinate dehydratase activity" evidence="23">
        <location>
            <position position="1190"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; for 3-dehydroquinate dehydratase activity" evidence="23">
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            <position position="1219"/>
        </location>
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        <location>
            <begin position="43"/>
            <end position="45"/>
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        <ligand>
            <name>NAD(+)</name>
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        <location>
            <begin position="79"/>
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        <ligand>
            <name>NAD(+)</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="110"/>
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        <ligand>
            <name>NAD(+)</name>
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        </ligand>
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            <position position="115"/>
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            <name>NAD(+)</name>
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        <location>
            <position position="126"/>
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        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
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            <begin position="135"/>
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            <name>NAD(+)</name>
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        </ligand>
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            <position position="142"/>
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            <position position="148"/>
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        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
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        <location>
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            <name>NAD(+)</name>
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        </ligand>
    </feature>
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            <position position="186"/>
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        <ligand>
            <name>NAD(+)</name>
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        </ligand>
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            <begin position="190"/>
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            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
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        </ligand>
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        <location>
            <position position="190"/>
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        <ligand>
            <name>Zn(2+)</name>
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            <note>catalytic</note>
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            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
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            <note>catalytic</note>
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        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
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            <name>Zn(2+)</name>
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            <note>catalytic</note>
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        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
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        <location>
            <begin position="882"/>
            <end position="889"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004811"/>
        </source>
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            <dbReference type="ARBA" id="ARBA00004842"/>
        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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            <dbReference type="PIRNR" id="PIRNR000514"/>
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            <dbReference type="Pfam" id="PF00275"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF01488"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="OEJ90953.1"/>
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    </evidence>
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    <sequence length="1583" mass="175129" checksum="980BC553F4CE8735" modified="2017-01-18" version="1">MSKINKVEILGKETIHVGYNISSHIVDTILKDCKSSTYFMVYDSNVEQVPYLQELQTQLKSKLDKKSQRLLTYVVAPGEANKTRQTKAQIEDYMLVQGCTRDTVVLAVGGGIIGDMIGFVAATFMRGVRVVQVPTSLLAMVDSSIGGKTAVDTPMGKNFIGAFWQPQFVLIDIKWLETLPKREFINGIAEVIKTAAIWNAQEFQRLEENADDFLNVVNNQKPLVGHKNVLFTNISPILDHIVKLVLESCRVKATVVSSDERETGLRNLLNFGHSIGHAYEAILTPQALHGECVSIGMIKEAELSRYLGILSPTQVARLSKILSAYGLPVSPDEKWFQDLTLKKKTPLDVLLSKMSIDKKNDGSKKKVVLLETIGKCYGTSAHFVSDRDLRVVLTEEILVYPFDLKKPVCKTIIPPGSKSISNRALVLACLGKGVCKIKNLLHSDDTKHMLAAVANLKGATISLEDNGETVVVDGKGGLLESTTEPLYLGNAGTASRFLTSVAALVQPSKETENLVLTGNARMQERPIGPLVDALRVNGVEIEYLNKEGSLPLKINTEKSVFKGGKIELAATVSSQYVSSILMCAPYAEKAVTLSLVGGKPISQLYIDMTIKMMQKFGVDVTKSTTEAYTYHIPKTQYTNPPEYVIESDASSATYPLAFAAMTGSEVTVPNIGSESLQGDARFAVEVLEPMGCKVVQTATSTTVTGPPVGSLKPLKHVDMEPMTDAFLTACVVAAVATNNKESGNNITTIEGIANQRVKECNRILAMITELNKFGVTATELPDGIQVEAVDKLTDLKVPKAVDTYDDHRVAMSFSLLAGLVQRTTPIRILERKTTGKTWPGWWDVLHTQLHAKLDGAEKLDPNLSLTKSHNVQPERPSVVVIGMRAAGKTTLSKWCAIALGYKFLDLDLEFERVNCKKGETIKEYVAAHGWDKFRAEEASLFERIIAQIGRQGYVISSGGGLVENSDSRVALKKFAFKDNGIVLHLHRDIDETVNFLNKDPTRPAYVEDIRTVANRRERWYQECCNFIFYSPHCETPVQFQQLRKSFEIFAQRMVGQLQDKIPTKRSAFVCLTFDDIETQDPATLRAITYGCSAVELRVDHLKNFSPEYVNRQISILRMATDSFPIVFTIRTKKQGGLFPDEDYKGIYALYKMAMKAAVEYIDLELTLPTDIQNSVFNERSQCTKIISSHHDFPGEFPWTHSEWEERYNQALSINADVVKFVGTATKFEDNLLLEEFRAKHTSKPLIAINMTARGKISRVLNTILTPVTSELLPAASAPGQLTVKQINHLYTELGGDLTPKHFYVVGKPIGHSRSPILHNTGYEVLGLPYKFDKFETDSASEVKSKLLSDPSLGGLAVTIPLKLDIIPYMDELSEAAKFINAVNTVIPLGNNKFKGDNTDWLGIFNSLTANGVPSKLPPKTAGLIVGAGGTSRAAIFALHKLGCHKIFMVNRTTSKLHDLKKAFPERYNIEVVETIEDAKKVEENFSVVVSCVPADKPLDETLIAQLEVLLQKNDFKELGFTSTLLEAAYKPRYTPVMRLAKEKYNWNVVPGVEMLVCQGVQQFEKWTGYKAPFAPILHAVTED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZA4</accession>
    <name>A0A1E5RZA4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">DNA repair protein RAD10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g251</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERCC1/RAD10/SWI10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000002">
        <property type="protein sequence ID" value="OEJ92129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZA4">
        <property type="interactions" value="135"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZA4"/>
    <dbReference type="InParanoid" id="A0A1E5RZA4"/>
    <dbReference type="OrthoDB" id="10262814at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070522">
        <property type="term" value="C:ERCC4-ERCC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000110">
        <property type="term" value="C:nucleotide-excision repair factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22325">
        <property type="entry name" value="ERCC1_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10130:FF:000015">
        <property type="entry name" value="SsDNA endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047260">
        <property type="entry name" value="ERCC1-like_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004579">
        <property type="entry name" value="ERCC1/RAD10/SWI10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00597">
        <property type="entry name" value="rad10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12749:SF0">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12749">
        <property type="entry name" value="EXCISION REPAIR CROSS-COMPLEMENTING 1 ERCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03834">
        <property type="entry name" value="Rad10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC1-like central" evidence="8">
        <location>
            <begin position="83"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="23"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ92129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="204" mass="23293" checksum="4B508A75515C6F09" modified="2017-01-18" version="1">MNNSDTTSFKSILENAKKFQQLKNSNEHVEQQTRNTTPPANKAGHIVNSFNRTGERTEQSGSSTYASKSSFQKPQFKSSESKTILVNKSQKGNPLLDKFANVNWRYVSSSSSXLEKIDYDYMVRGKRKVIFLSLKYHKLHPEYIVKKLKPVANQGAIMLCVVDIEKSENILKDLNKLCLFNGFTLLLSFNFEQAAKYLMYMNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZJ1</accession>
    <name>A0A1E5RZJ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Putative ATP-dependent permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g142</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZJ1">
        <property type="interactions" value="332"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZJ1"/>
    <dbReference type="InParanoid" id="A0A1E5RZJ1"/>
    <dbReference type="OrthoDB" id="66620at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF2">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="363"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="820"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="853"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="909"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="941"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="968"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1054"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="420"/>
            <end position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ92392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1080" mass="121345" checksum="721F87B6BEC89BBE" modified="2017-01-18" version="1">MVALNNYCPCRDFNDDLNYLGKTTINSIPTGQSKNMKTTQGEPHFFRLIVFLLAVTAIATATNAFDSRHSGNLKTFDLQNIPRRKPTPGDDVCPPCFNCMLPLFECKQYSSCNEYNGRCECIPGFGGEDCKEALCGGLSSENNQRPIRNVSEGFCKCEDGWGGINCNLCQSDDVCDNFLPGEVKTGAVCNKNGMIVNKMYQGCDVTNEKIKSILGDKSPQVTFSCNRTSEICDFQFWVDQVESFYCGLDTCKFEYDIAKNTSHYNCDNAKCKCLPGEFLCGKSGSIDISDFLTETIKGPGDFSCNLETRKCIFTEPSMNDLISTIFGDPYIMLQCESGECIHYSEIPGYLSPEKPPISLKQKIILITTSIALVMFICVIAKCILVSPLFTGENKNIEDPKSDGERIEDLEFLSNTMGATLTFENINYFASNQHILKQVSGVIKPGLTAIMGGSGAGKTTLLDILALKNKCGKTKGSIKINGEEVGHTNKKFKKXISKTIGFVDQENFLLPTLTVYETVLNSALLRLPKIMSFEEKQKLVIKVMQDLRIYDIKDRIVGDDFSRGISGGEKRRVSIACELVTSPSILFLDEPTSGLDSNNAKNVIECLERLAVLYNRTIVVSIHQPRSNIFQKFDKLILLGNGEMCYSGTTLDVRDFLKNCGYECPRDYNLADFLIDVTFETPLTETSEDQEENFENSAHDHAHSHALLSPNTDTAIQGEWAHLAEHRDELRSLLASTNKSKYNTIDDASKKNILTSSMLSSKFSESQFYADLNFQIKNANASVSETTSELAKRQVPKASFWQQLVILSSRSFKNIYRNPKLLLGNYMLSLLMGLFLGFLYYGVSNDISGFQNRLGLFFFIITFLGFSSFTGLSSFNLERLIFLKERSNNYYHPLAYYLSKVISDIFPLRVVPPIILVLIIYPLVGLNMGLGSGIAYPFFKXIEILIMFNLAIAFLIQSIGFFFQELSNSIIFSVLVLLWSILFSGLFINTQEITNIFFKYMKNGSIFYYAYESLIINEVKDLMLKEKKYGLNIEIPGATILSTFGFHVQHLTLDISILIAFMVFFFFFGYVILKWRVVENR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZQ1</accession>
    <name>A0A1E5RZQ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein AIM2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g232</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZQ1"/>
    <dbReference type="FunCoup" id="A0A1E5RZQ1">
        <property type="interactions" value="249"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZQ1"/>
    <dbReference type="InParanoid" id="A0A1E5RZQ1"/>
    <dbReference type="OrthoDB" id="17560at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17630">
        <property type="entry name" value="DIENELACTONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17630:SF44">
        <property type="entry name" value="PROTEIN AIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dienelactone hydrolase" evidence="1">
        <location>
            <begin position="53"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="263" mass="28796" checksum="1F6BC73941C1155C" modified="2017-01-18" version="1">MASNPPAACCFEGFLHEGTPKGVHKELFGLNTYISTPTDPSKCKGCECGGSEPKECEKVIVMLTDVFGNTLTNCLLIADDLANQGFKVLIPDILLGETIDITKVDKVNFQDWMSRHENSITKPVVDAFMQKVKAEMKPNFLGVVGHCFGAKFAVQQLDHSAENKGVADCAAVAHPSFVTMEELAAIEKPLLISAAENDDIFPAELRHQSEDKLKEIGATYQLDLFGGVSHGFAARGDVSDPKVKYAKEKVLLDQIYWFKHFSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZU1</accession>
    <name>A0A1E5RZU1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g73</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZU1"/>
    <dbReference type="InParanoid" id="A0A1E5RZU1"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ92492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="82" mass="9576" checksum="7BEAC0F4D9222E3C" modified="2017-01-18" version="1">MSNKSTSLTFKSATTHQYIHNKSLYYELFNSTQDTNKEYIETEQYTRNTPLLERCKTVHERLLEMTKNSKEPSSPLASTIPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S066</accession>
    <name>A0A1E5S066_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tyrosine-protein phosphatase CDC14</fullName>
            <ecNumber evidence="4">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g177</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5S066">
        <property type="interactions" value="902"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S066"/>
    <dbReference type="InParanoid" id="A0A1E5S066"/>
    <dbReference type="OrthoDB" id="5632at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032954">
        <property type="term" value="P:regulation of cytokinetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007096">
        <property type="term" value="P:regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17657">
        <property type="entry name" value="CDC14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000038">
        <property type="entry name" value="Tyrosine-protein phosphatase CDC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000073">
        <property type="entry name" value="Tyrosine-protein phosphatase CDC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029260">
        <property type="entry name" value="DSPn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050561">
        <property type="entry name" value="PTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23339">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASE AND DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14671">
        <property type="entry name" value="DSPn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0469">Meiosis</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="16">
        <location>
            <begin position="187"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="17">
        <location>
            <begin position="262"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="374"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="439"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="537"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="380"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="394"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="554"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ92449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="594" mass="67923" checksum="D43161B3FB95F659" modified="2017-01-18" version="1">MAKKLYLDNTIEFLRGRVYLGAYDYIPEDTSDVTFFTMEEELFYNSFHLDFGPFNIGHLYRFAVKLHDLLTREENLSRAVVFYSSTSTRQRANAACLLCCYMILIQSWTPHQVLQPISQVDPPFMPFRDAGYSNADFEITIQDICYGCWRAKECGLIDLQKMDLDSYEYYERVENGDLNVLTPNXIAFASPKENVADMNNNHNTKINRTFRKVLDIFQKTNVQLVVRLNSHLYNKVHFENNGIQHLDMIFEDGXCPDLSIVTNFIGXVETIINEFDDLNDQAPKIAVHCKAGLGRTGCLIGAYLXYKHGFTANECIGFLRVIRPGMVVGPQQHWLYLNQQTFREWKYTMKMSIKPSDLINGMYPLISVEEYKKQKKERLQQQQQQQQQQQKQQQQHHHHHHHGKKLQLLQHERKTSNNDEGLDENSKEIDQADLSGIIIPPETASPMTNSLRHGVPMRSPGQPRKGQHGANTIEDINSLPGKRAKSKTAIVNKNSXSNNNANTTQENLENEADVSMTSRRNITPIDDQDMKMLYVAKQQQRRSGGRNLSSSSSAMTNTMHSANHLGQNGGNFSRAVSGGNXRKISGSYRKDSKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0E4</accession>
    <name>A0A1E5R0E4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R0E4"/>
    <dbReference type="OrthoDB" id="3972502at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="576"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="618"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="860"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="580"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="619"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="636"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="860"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="891"/>
            <end position="901"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="925" mass="105534" checksum="3F81E4AE321A62DF" modified="2017-01-18" version="1">MMNENVATIGVGSENINKSISHKKTPQIRYDKLDLFYDEETTLPVKYPFRSYLLCSNDTKIHYDTINNTINTISMGTPLHSVRQAKTRKKRRILQTYARPQRTVGYSETDDFEAAVEKNEEVSVLLECKKNTSLTDFEFMXXQSEDFSKNNNSNLXMTKTTAKNLKRTRMPNKNKKNKLTDSASEEENENGIDFNSANGTQKKRKYNKGKAXNKLLKPRPKRLIKSRMQKQEEQDELDEKNTLYVKQMKKLKAAREKEKNIKEAIRKLEKGLPLTSSTISASSLLAEVSSSDNEDFFDIEYTEAGEDGKKDDLRPLYPQAPPSKSSRKIPEPNFNQFNEDLWDIPPNVLIRKLSIKDTVAKTSDELRREAFLLEMLKKRAEXPQQXDEELKDRPEVDGGTDFEQVKDVDFEGYDDFIIVGADAYSPEMLHEMQXVKKGRKIRKDYVLPPEPSCVTKDLLLEGRKWRAARMYLLNPLRTSLREVCKENNVSVPTLITYMDATRVARSLMTGKYSGSSVIRDSTFNNRGKNKFPKEVRDQFLKRAYEKLNEAREKTAQITTDLGLRDRHRGLRLFFDSRGTSGSQLSPGPSASSNSXAFSNMDSKXLSGSPLGVEADFFPSSSSSSSTTTSTTFSSSQHTNQINDSTVYDKNNTVELYLPNEDVTIVDVTGNAKSEELSGELKPLEFIRPRKKKTPEPNLQRKKIQINDVSFEPSKVPDVELAKKTPDRAFIEENPAKTIFEKTKQDESFFKRRSKKRSVVKHDESDEKTFKEPIQSMFFKPNKRLLNEINQNESVPKTINIVNENSKKAFVVKPYELDKEKLKVPIPTISLKPGVQTFSKSSSKGDQLRKLFKRIIKQRDELKNDNSSEKDSGTALENHAAPKEAVSXSTEQPKDIEKKDSLESVQXLDPVTQPKAETQIDKQXTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0F0</accession>
    <name>A0A1E5R0F0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ZZ-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4512</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0F0"/>
    <dbReference type="InParanoid" id="A0A1E5R0F0"/>
    <dbReference type="OrthoDB" id="661148at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016235">
        <property type="term" value="C:aggresome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044753">
        <property type="term" value="C:amphisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070530">
        <property type="term" value="F:K63-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005080">
        <property type="term" value="F:protein kinase C binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035973">
        <property type="term" value="P:aggrephagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02340">
        <property type="entry name" value="ZZ_NBR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024543">
        <property type="entry name" value="Atg19/Atg34_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052260">
        <property type="entry name" value="Autophagy_Rcpt_SigReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15090">
        <property type="entry name" value="SEQUESTOSOME 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15090:SF0">
        <property type="entry name" value="SEQUESTOSOME-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12744">
        <property type="entry name" value="ATG19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="6">
        <location>
            <begin position="68"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="143"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="447"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="457"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="498" mass="55596" checksum="6EFA7A110C773991" modified="2017-01-18" version="1">MSEPTIGIPLTQWNDLLTHLNDIAESLKTSHQAASTEGVSGKTAENPNNQAYVPVHKYVYCDGKCDKEITESANDFHQEPIKGDRYKCLECYNYDLCSSCEDAKVESGSHKSFHNMVKIKTPDSYRSQSAYMAAPFFGSSSSSXESESAAQNNGNVSASTQSKTSCKSSGTHTDVVFDISDKDKDLFDFFSKLDTVEKLRGVMNKVVNYDELLIQYEALLPKTLPKSVFPTPSAKTAESFSSSTLTSSKDGFSTTPNFSGKLGNPGIYVDFGIKNKKEMVFKILNSSNNITIPTNCILNFNFVRKSTHKMVKCYLQMSKSQEVKPLDSFKINFNHVFQDGDFLNESEHFEVKIMHKSKFFKXPVVLYEAFTKTLAHPVLLNPSVEGKTALKQEAARENXVKTQQSSTKISYXSENVTFSPDVLSVEETLKKIERGILFHDTKGTFADTPASRLDNDVPTDDDNERADESDEENISDDLQSAYEEELDDYDILSNSDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0S4</accession>
    <name>A0A1E5R0S4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cohesin subunit SCC3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4592</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0S4">
        <property type="interactions" value="245"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0S4"/>
    <dbReference type="InParanoid" id="A0A1E5R0S4"/>
    <dbReference type="OrthoDB" id="498590at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039662">
        <property type="entry name" value="Cohesin_Scc3/SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048610">
        <property type="entry name" value="SCC3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020839">
        <property type="entry name" value="SCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013721">
        <property type="entry name" value="STAG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199:SF0">
        <property type="entry name" value="LD34181P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199">
        <property type="entry name" value="STROMAL ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21767">
        <property type="entry name" value="SCC3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21581">
        <property type="entry name" value="SCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08514">
        <property type="entry name" value="STAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51425">
        <property type="entry name" value="SCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCD" evidence="2">
        <location>
            <begin position="375"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1099"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1099"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1123"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="594"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="1124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1143" mass="131626" checksum="7AD26026340E26BC" modified="2017-01-18" version="1">MTTVRRSTRIKNTSSTARSQLISEDTETVDTRYGNLTDXDEHDRDVSGLESEESSEEDNDDNADEDYVXKPKRSTSSSKKPRKRQSTGKTTKSHKRAKTSKQPVSNEERTKYEENYQNLLDSFQPTTLFTTMCTSENTLALSELVHDWLDVYAESPNRAKQQFVNFILNCSGSLIQVEEHDVANSDSANETVSEVQLLFHEQKFHEFYLLNSYLHKNQHKEYKYLYENFVEFLDHLIKISYEKGFLCVAVEEEVAKENENENGDTGGAQQQVTIGMGPMILDLIIWLSSLSVSNFRCFRYTASLALYVFETALVQLIPTIETDXIQNLKQLHDRESKKKRPNKVAMEQWKSQLQEEQEHKTTIEKIITEIFKVSFVHRFRDIDEHVRSNSIYYLSQWFKAWPEHFYQVTYLKYFGWLLNDENAQVRNQVLSSLADLVKFSNSRNSISIENECFRQFFERFQDKIKQITCIDSSYDCRMHAIKVLTQINHMDYLEEPDRLAMSSLLFKIDSTDDKFSASTSKESRFYSAVAKMFYDSECFTELHIPLKKSKTKSKTKSDAGKANEFSQGIKFIKYLLEALSCHISISKKISTYDDFSLNDVEIEFTSFAEYSLKIYQACQFLSLHYKHLIPILCQMLTYTGNFENLYDIDTDEFDELLMPNNKVSRELYVIVLSGLCMGNRRAAVSSTELKXTLADDLPVLMKELDYRSSPIDVIYTFKILTLFNFESFSENSLSEITHHVRSLFMSISLNDVQLKNAFQDVFEFYKHKIGSNIYDQIWVSTVNEMLEKAKHFLETNFDQKQTSFNDNIKQLFEVXINKLVVLGKFYKLPFDEDFVTLFISKVFESLVDEVDDLDSQVLDFKIFPLIISWSLLDLESKELDYTQAVNDLGETGAVTVPAFPSDLFKSVHSFVQYLVKTEERLFALVGDESLNVLLLKLSSTVMDLLISLKAFSLNCSIDEWAETIDDDYDFTLHKNGYVKVLYTVFCFLESKAAGQLEVETLERLSGEDTFYDQYLGQTAESSEKDITSSDGAVVTRNSINFSDLCVFTIKLKTLQKLSIFDENASEAKKLIKRLSLNKNVFGSVFSNIVDDNVSNQRSTVPSAATTTMPSSSKQPIAHRQALEDPDNDEFAVDPIEDSESLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R190</accession>
    <name>A0A1E5R190_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Prohibitin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4573</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Prohibitin probably acts as a holdase/unfoldase for the stabilization of newly synthesized mitochondrial proteins. Involved in mitophagy; may act as an adapter for ATG8 that supports mitophagosome assembly. Negatively regulates the proteolytic processing of ATG32 via the i-AAA protease. Acts as a negative regulator of the m-AAA protease.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The mitochondrial prohibitin complex consists of two subunits (PHB1 and PHB2). The subunits assemble into a membrane-associated ring-shaped supercomplex of approximately 1 mDa. The mitochondrial prohibitin complex interacts with the m-AAA protease, a heterohexamer composed of YTA12/RCA1 and YTA10/AFG3. The mitochondrial prohibitin complex interacts with ATG8 and the interaction may support mitophagosome assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the prohibitin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R190"/>
    <dbReference type="FunCoup" id="A0A1E5R190">
        <property type="interactions" value="869"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R190"/>
    <dbReference type="InParanoid" id="A0A1E5R190"/>
    <dbReference type="OrthoDB" id="275637at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045861">
        <property type="term" value="P:negative regulation of proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03401">
        <property type="entry name" value="SPFH_prohibitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000001">
        <property type="entry name" value="Prohibitin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000163">
        <property type="entry name" value="Prohibitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222">
        <property type="entry name" value="PROHIBITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222:SF0">
        <property type="entry name" value="PROHIBITIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00679">
        <property type="entry name" value="PROHIBITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band 7" evidence="6">
        <location>
            <begin position="26"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00057247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00063239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="286" mass="31186" checksum="7E6C87F7754D64AD" modified="2017-01-18" version="1">MSQRLVENLGKVIVPGIIGVSLLQNSLYDVQGGSRAVIFDRLSGVSQTVVGEGTHFLVPWLQKSIIFDVRSKPKNIATNTGTKDLQMVSLTLRVLHRPDIMKLPAIYQTLGQDYAERVLPSIGNEILKSVVAQFDAAELITQREIVSQRIAQELQNRSAEFNIKLEDVSITHMAFGPEFTKAVEQKQIAQQDAERAKFLVEKAEQEKRAAIIRAEGEAEAAEFISKALAKNGDGLLSIRRLEASKDIARTLSASGNITYLPGGVNGSNEGHGSNGTPNSLLLNIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R195</accession>
    <name>A0A1E5R195_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mso1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4308</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R195">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R195"/>
    <dbReference type="InParanoid" id="A0A1E5R195"/>
    <dbReference type="OrthoDB" id="3973185at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028095">
        <property type="entry name" value="Mso1_N_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14475">
        <property type="entry name" value="Mso1_Sec1_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Mso1 N-terminal" evidence="4">
        <location>
            <begin position="26"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="79"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="127"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="173"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ80647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="215" mass="24284" checksum="E3A28861CC2B7619" modified="2017-01-18" version="1">MSSSHNNSIWSSFKTKGRSLQSSFHQLNIKHSTEKDGDSSESTLVHKALVTYYKKREPFEGFPDWLGHKEEINVQDQLQKHHPHSAGVVPATPEEAESGFSHGAPTSSTSSSKPLSSMRRKLHLGGSKQQLSTAEQQRLARPTFQGMFDQEYSPPASSNGVXDTEVLFHQQEEQQQQQQQQQQQQQHSAPLPEQPAVNRMPSTSQLMRDRMKRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1C1</accession>
    <name>A0A1E5R1C1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">37S ribosomal protein YMR-31, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4378</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alpha-ketoglutarate dehydrogenase component 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1C1">
        <property type="interactions" value="99"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1C1"/>
    <dbReference type="InParanoid" id="A0A1E5R1C1"/>
    <dbReference type="OrthoDB" id="3972894at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006103">
        <property type="term" value="P:2-oxoglutarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020373">
        <property type="entry name" value="Kgd4/YMR-31"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10937">
        <property type="entry name" value="Kgd4-YMR31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ80686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="119" mass="12717" checksum="DE497AFE1BBB824D" modified="2017-01-18" version="1">MIKFVGGPHTFDSASTAAKTHXCAPNGLLPNSAECIGVAELMKSWKPLXIKPYQGKSSSTSKTSSSGGSNSKTATKYEYTKRPLQKGEVASILDLPQQYRFKPIDEQEMDIIMGGGAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1G8</accession>
    <name>A0A1E5R1G8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Exosome complex protein LRP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1G8"/>
    <dbReference type="FunCoup" id="A0A1E5R1G8">
        <property type="interactions" value="316"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1G8"/>
    <dbReference type="InParanoid" id="A0A1E5R1G8"/>
    <dbReference type="OrthoDB" id="1421013at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007146">
        <property type="entry name" value="Sas10/Utp3/C1D"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04000">
        <property type="entry name" value="Sas10_Utp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="206" mass="23448" checksum="D1E94BC16F550DE0" modified="2017-01-18" version="1">MDDTEKFKKYLKYLNKQLDKFQNDVKAKVLNKSLDDRLNVELSGNDKSLERLQTCNELAYLLSSLVFIYQKLNCDKDMSNVVRELGRIQSYMSEYEQLQKKNSSSSSSSSGTRGENTNEETPVQSSISQQNFSKSTQPQGVHIKFQDMNNNNFSADTSKESTNEYKDNEIKDVSSQILEKLSKKHAGSGGMKKKKGIMKAKRVSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1I5</accession>
    <name>A0A1E5R1I5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Mitochondrial translation optimization protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4364</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the MSS1-MTO1 complex that catalyzes the 5-carboxymethylaminomethyluridine (cmnm(5)U) modification at the 34th wobble position (U34) of mitochondrial tRNAs.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MnmG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1I5">
        <property type="interactions" value="771"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1I5"/>
    <dbReference type="InParanoid" id="A0A1E5R1I5"/>
    <dbReference type="OrthoDB" id="3329at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070899">
        <property type="term" value="P:mitochondrial tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.570:FF:000001">
        <property type="entry name" value="tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000002">
        <property type="entry name" value="tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.570">
        <property type="entry name" value="GidA associated domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1800">
        <property type="entry name" value="tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG/GidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049312">
        <property type="entry name" value="GIDA_C_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004416">
        <property type="entry name" value="MnmG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002218">
        <property type="entry name" value="MnmG-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020595">
        <property type="entry name" value="MnmG-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026904">
        <property type="entry name" value="MnmG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047001">
        <property type="entry name" value="MnmG_C_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044920">
        <property type="entry name" value="MnmG_C_subdom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040131">
        <property type="entry name" value="MnmG_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00136">
        <property type="entry name" value="mnmG_gidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806">
        <property type="entry name" value="GLUCOSE INHIBITED DIVISION PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806:SF0">
        <property type="entry name" value="PROTEIN MTO1 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01134">
        <property type="entry name" value="GIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21680">
        <property type="entry name" value="GIDA_C_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13932">
        <property type="entry name" value="SAM_GIDA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01228">
        <property type="entry name" value="GIDA_assoc_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01280">
        <property type="entry name" value="GIDA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA uridine 5-carboxymethylaminomethyl modification enzyme C-terminal subdomain" evidence="6">
        <location>
            <begin position="603"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="690" mass="76610" checksum="1BFC1946256DD98A" modified="2017-01-18" version="1">MLFVKRPLVAQFLKRSLGQRSIHINAELKNLLEKSENYGVVVVGAGHAGIEAATGAARVLKDTLVPTTLITPFLSKIGTCSCNPSMGGVGKGTLLREVDALDGVAARITDLAGIQFKMLNRESGGPAVWGPRAQIDRDIYLKEMQKEVLNYKHGNLNVKEGKVKDIIISEXNAIVGVILEGDGPTLTNKQQQXHGTVMKCSQVVITTGTFLSAEIHIGMRTIPAGRIGEEPTYGISKTLENFDFNLGRLKTGTPARLKKSSIDFSQLTKEYGDLKPIPMSYMNEKVAVEEQILCYGTRTTPQLHKYLSENLXNSIHFIKETVNGPRYCPSIEAKITRFADKESHKIWLEPEGLTSDVVYPNGISNSMPEDVQLKMMRMVPGLENVEIIQPAYGVEYDYVDPRSLTSTLETKSILGLFLAGQINGTTGYEEACAQGCLAGINAGLQALGTKKKLILKRSDGYLGILVDDLITKGVSEPYRMFTSRSEFRLTSRAXNADLRLTELGRNVGCISSARWNKFSTDKTTYQEIISKLKALSKSSDGWNKNLPAPFFVRDGHTKKSAWDLLKLNGMNLEHLAEAFPELQIDLKHGNVPQHVVTKINVEGKYEPYLTKQRHYIKAFSADEAMLLPVDYDYQNMKTLSHECRNLLNTVKPTTIGQARRIQGITPASIYELYRLVKKQQPSLRRPANKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1P4</accession>
    <name>A0A1E5R1P4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Family of serine hydrolases 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3873</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1P4">
        <property type="interactions" value="161"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1P4"/>
    <dbReference type="InParanoid" id="A0A1E5R1P4"/>
    <dbReference type="OrthoDB" id="2094269at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005645">
        <property type="entry name" value="FSH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050593">
        <property type="entry name" value="LovG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070">
        <property type="entry name" value="ESTERASE OVCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070:SF9">
        <property type="entry name" value="FAMILY OF SERINE HYDROLASES 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03959">
        <property type="entry name" value="FSH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine hydrolase" evidence="2">
        <location>
            <begin position="3"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="235" mass="26555" checksum="5DA103E64E6AD81F" modified="2017-01-18" version="1">MSSKILCLHGFLQNAKVFSEKSSGIRKLLKKNNIQTEYLDGPTVLSKQDLPFTIDDDKWEAIEKDQLNKSWFYHTDISSELNIETAINTVSEYIKEHGPFDGILGFSQGAALAAIVTNQIKTLVPDHPYFKVSILFSAYSFTEKDPNSADANALRITPKYQDSFQPPKDLSTKVIFIYGLNDNAVPGERSKFLYNIYNXTEGVDVKFFEHDGGHLVVNKKDFLRPVVEEITEALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1Z2</accession>
    <name>A0A1E5R1Z2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL40</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4146</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL40 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1Z2"/>
    <dbReference type="FunCoup" id="A0A1E5R1Z2">
        <property type="interactions" value="279"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1Z2"/>
    <dbReference type="InParanoid" id="A0A1E5R1Z2"/>
    <dbReference type="OrthoDB" id="3970694at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3440:FF:000001">
        <property type="entry name" value="Mitochondrial ribosomal protein L40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019192">
        <property type="entry name" value="Ribosomal_mL40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042831">
        <property type="entry name" value="Ribosomal_mL40_fung"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39150">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L28, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39150:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09812">
        <property type="entry name" value="MRP-L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="161" mass="18852" checksum="2AD65BB0DBA3A7D3" modified="2017-01-18" version="1">MLKSTVFATAKNCTSSSALFPAKPMSMTFVRYKRTKGKELSSNVQKAITQMSVLSANRKQPKRLKLCPEDLIKHETIQRCWAEYQRDLRTERNQCLEAQYASMDEAMSVLEQLSPELYTAANKKDMKHRFPKEFRIPTEYPPNKIWYYEYQEGDGNTHKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R205</accession>
    <name>A0A1E5R205_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">26S proteasome regulatory subunit RPN12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g4386</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R205"/>
    <dbReference type="FunCoup" id="A0A1E5R205">
        <property type="interactions" value="1246"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R205"/>
    <dbReference type="InParanoid" id="A0A1E5R205"/>
    <dbReference type="OrthoDB" id="8775810at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006746">
        <property type="entry name" value="26S_Psome_Rpn12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033464">
        <property type="entry name" value="CSN8_PSD8_EIF3K"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12387">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12387:SF0">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10075">
        <property type="entry name" value="CSN8_PSD8_EIF3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="78"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="278" mass="31959" checksum="375A150786A8FBD7" modified="2017-01-18" version="1">MVSLSELTNALKQAVDNSNYSACEQLLPSIKLELIKNNLLVPDLQKSLVDESYFQDLCVSRSITECACICSINLEQFDRFKQFFTLLRPYYFNDINPKLNHSNNKNKLISLYLLDLLSQGDVTTYHEELEFFSKKLNNIEEDEYCSYPIKVEKWLMEGSYQKVKDILAVGGDKEEFKFFSEILLNQIREEIALNTELAYKTLSLSTAKALLFLNTEKQMEQFALDQGWRIEKGVIVFDQNGSQQEDVEIGDTVKGNQDNTHKIISQVLGYAVNLETIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2F0</accession>
    <name>A0A1E5R2F0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome c oxidase assembly protein COX16, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Cytochrome c oxidase assembly protein cox16, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g4051</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the assembly of the mitochondrial respiratory chain complex IV (CIV), also known as cytochrome c oxidase. May participate in merging the COX1 and COX2 assembly lines.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the COX16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2F0"/>
    <dbReference type="FunCoup" id="A0A1E5R2F0">
        <property type="interactions" value="144"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2F0"/>
    <dbReference type="InParanoid" id="A0A1E5R2F0"/>
    <dbReference type="OrthoDB" id="5516033at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020164">
        <property type="entry name" value="Cyt_c_Oxase_assmbl_COX16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17130:SF14">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX16 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17130">
        <property type="entry name" value="MITOCHONDRIAL OUTER MEMBRANE PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14138">
        <property type="entry name" value="COX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ81087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="119" mass="14030" checksum="E75C38D35DEE3540" modified="2017-01-18" version="1">MGSTFGQKVFRSKRQEALYNASIPGKYRKAFEKRPFFFFGLPFLAIIGLSSYWLSGLTSVRYEKHDAKQCEIDAQELIDLPKRKFDMKEEYYRLQGIGLDDWEQKRVPRMKGESDNVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2I8</accession>
    <name>A0A1E5R2I8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sterol esterase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3928</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2I8">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2I8"/>
    <dbReference type="InParanoid" id="A0A1E5R2I8"/>
    <dbReference type="OrthoDB" id="6130531at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006693">
        <property type="entry name" value="AB_hydrolase_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04083">
        <property type="entry name" value="Abhydro_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Partial AB-hydrolase lipase" evidence="3">
        <location>
            <begin position="143"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="67"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ81111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="538" mass="62489" checksum="271321276E3A0BE8" modified="2017-01-18" version="1">MYATLYAITGWIITTQFLTALFVFCILNHYLKPRYKRRDTKPIKTKILEPEKIILVKADNNEHIGKNTXTRQXLRNNNNNNNNESSSTSRPTSSTPLYNHSETESIENIAVGQTDDNGAELKILENPFEMASEPTYKLVPDLNYYFQQYGLTIDSYRLSTEDGFIIDIWHIVNPNATNTRDSDSRPVLFLHGLLQSSGSFASSGTKSLAYYLTVHGGKDCWLGNNRCGFVPGWEDLTINSMGEPENKKKTKKERWAWDILDMAKYDLPCLVDFVLKETNSKNLDLIAHSQGTMQSFYSLTNHRELLGKIKNFVALSPAVYPGALLYEKIPLRLMALLIDNKWIFGKTVFIPLMMQMRKLMGSQSSYCKLAYLMFNYLFDWGDSLWDRHLRDRHFLFSPVYISVRLMQWWLSSKRPSFRNGEFDKTYFPEHKVWYPTDFHNAEQESRNSSSTATFQKTATRAKSTHIHNNSDKNLYPNFLIFAPRLDRLVDGARLINHFTKYEDPALYKIWYIEHYSHLDVLWADDVLEKIGKPILDHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R378</accession>
    <name>A0A1E5R378_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ethionine resistance-conferring protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4233</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R378"/>
    <dbReference type="InParanoid" id="A0A1E5R378"/>
    <dbReference type="OrthoDB" id="2126698at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="382"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="415"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="441"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ81023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="481" mass="52629" checksum="76FF209B7555D392" modified="2017-01-18" version="1">MSSTLAAASPVGYGSISDQKNQPESFNEIKELLYADKREEIVKITKTSVPLAFTFLLQFLITTITVVTVGRIGTIELGGVSIANVTYSVSTAIFIGLATCLDTLCPQAYGRGNRTLVLLYFLRCVMISLAAAIPIVLVWFKSAFLLKLMIQDEQVVAYASKYLKIMCLTAPGYIVFECGKKFLQSQNDFVTGQRLLWLGVPFNIGMNLFLIKHFGFIGAPIAVALTYNLIGALMLYKCSKTECWKEIANISFKQCFESWSTLXSLAIPGIIMLEAEFFAFEVLTVLAGRFGTTVLAAQSVAASLQSLMFQIPFSFSVAASNRIAYHIGSGDLHKCKLATKVTLINIACVLCAINFSLFTFLGNPLSSIFTXDKEVVKISCSILKIIAVNQLYDVFNILSAGCLRAQGRQKIGGYLNLISYYVIGLPVGIYLGFXTSLGVDGFWVGLGVGIFVLATAETYFVVRSNWNEIIERSKKLHGLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4V7</accession>
    <name>A0A1E5R4V7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">UDP-N-acetylglucosamine transporter YEA4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3692</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4V7">
        <property type="interactions" value="161"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4V7"/>
    <dbReference type="InParanoid" id="A0A1E5R4V7"/>
    <dbReference type="OrthoDB" id="999962at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005462">
        <property type="term" value="F:UDP-N-acetylglucosamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005464">
        <property type="term" value="F:UDP-xylose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013657">
        <property type="entry name" value="SCL35B1-4/HUT1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00803">
        <property type="entry name" value="nst"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778:SF4">
        <property type="entry name" value="NUCLEOTIDE SUGAR TRANSPORTER SLC35B4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08449">
        <property type="entry name" value="UAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ81920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="308" mass="34530" checksum="481EADA2C906A6B9" modified="2017-01-18" version="1">MGAADLLGYPAIQDTGSFITFCQFVYISIESVAVPFSHKRVVPLKVYMLSVLMFFLSSLGNNMALNYNISVPLHIVFRSFSPVITMINGSLFLKKKYNSSQILAALILTAGCIITSIYRNKEFXWADLHRLLSTNTSWLPAARKNSTGIVLLFGSSYLMSLLQLYNESTYKKYGKHIWKEMLFYTHFLSIPLFALTSLNSITNNLQLLKNNYLSRHFRPSWDSFPTTLVANLVTQSLCVKGVSRLSSSTNALTVSIVLMLRKMLSLLLSVWIFGNTMTKASVFGTVLTFLGTLLYSIGLFSNKASIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R521</accession>
    <name>A0A1E5R521_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Replication factor A protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3749</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the replication factor A protein 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R521"/>
    <dbReference type="FunCoup" id="A0A1E5R521">
        <property type="interactions" value="894"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R521"/>
    <dbReference type="InParanoid" id="A0A1E5R521"/>
    <dbReference type="OrthoDB" id="25571at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005662">
        <property type="term" value="C:DNA replication factor A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040260">
        <property type="entry name" value="RFA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014892">
        <property type="entry name" value="RPA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13989">
        <property type="entry name" value="REPLICATION PROTEIN A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13989:SF16">
        <property type="entry name" value="REPLICATION PROTEIN A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08784">
        <property type="entry name" value="RPA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Replication protein A C-terminal" evidence="6">
        <location>
            <begin position="220"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="109"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="116"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ81991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="281" mass="30343" checksum="19A45FBECD0BD537" modified="2017-01-18" version="1">MDGGYSGYSAPTDFNSDGGFIQGSSSQGNSSGAAFDAKKHLVPCTIKQLVDSAPALQDGTNICGNLNLTNVSIVGVVRTITDKDSFSVLTIEDGSGQIEFRIYHNTQPAEADGDSDMGMGDFDNNTNSSDDNKKEQYTVGEYVKVYATPRLIGDTLGLQYVVVQRINSYNEVVAHLLNAAKVYMISEGKINENGEKTNGGLKPDGSNNTGNSLFLQDDDQTPGQKIITLLHESKGSFPNGIPLRFISEKLGMSLDEVRDTTQNFIDEGLCYTTLEDHVELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R538</accession>
    <name>A0A1E5R538_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcription factor IWS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3645</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transcription factor involved in RNA polymerase II transcription regulation. May function in both SPT15/TBP post-recruitment and recruitment steps of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IWS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R538"/>
    <dbReference type="FunCoup" id="A0A1E5R538">
        <property type="interactions" value="365"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R538"/>
    <dbReference type="InParanoid" id="A0A1E5R538"/>
    <dbReference type="OrthoDB" id="3973229at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051037">
        <property type="entry name" value="RNAPII_TF_IWS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46010">
        <property type="entry name" value="PROTEIN IWS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46010:SF1">
        <property type="entry name" value="PROTEIN IWS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="5">
        <location>
            <begin position="214"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="70"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="337"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="100"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="128"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="157"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="346"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00037992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ82009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="432" mass="49487" checksum="2253730BC2108AC5" modified="2017-01-18" version="1">MEDNGLPEERVRKHIEESEDDEDVDVNVNNRVINNDDDEEQSYAGGNHLQPDYQAEGVVMDEHARKRHDLEVRMTQALKRQGRGSSRRSRRDEEDLEQMLDDKILRLKDEMTLAAQRDMETIERRINDDEEEEEEEDDEEDDEEDDEEDYEEDDEDREKTKKDSKGAHKNNKTKSNKKKEYQPAMEKVKLLPKVAAALNKVSLADTILDNNLLQAVKFWLEPLPDGSLPSFEIQKTLIQAIAQLPIKTEHLKESGLGKVMIFYTKSSRVEPSLKRLSDKIVAEWTRPIIGASDNYRDKSIMKIDFDVQKYKEKSTQELKKNLAKEKSARLALANGGADVSGAGVTKSKRSKRGSTKDGKKRSSLYDPGQQRKNRTVIPQQTTTDYKYAPVSNVDNLKDHQLSFKTSGVGSSLSNSELYKRINSRLGNTKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R594</accession>
    <name>A0A1E5R594_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="9">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g3671</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">ATP-dependent DNA helicase involved in DNA damage repair by homologous recombination and in genome maintenance. Capable of unwinding D-loops. Plays a role in limiting crossover recombinants during mitotic DNA double-strand break (DSB) repair. Component of a FANCM-MHF complex which promotes gene conversion at blocked replication forks, probably by reversal of the stalled fork.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with the MHF histone-fold complex to form the FANCM-MHF complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the DEAD box helicase family. DEAH subfamily. FANCM sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="9"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R594">
        <property type="interactions" value="246"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R594"/>
    <dbReference type="InParanoid" id="A0A1E5R594"/>
    <dbReference type="OrthoDB" id="164902at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045003">
        <property type="term" value="P:double-strand break repair via synthesis-dependent strand annealing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18033">
        <property type="entry name" value="DEXDc_FANCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12091">
        <property type="entry name" value="FANCM_ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000861">
        <property type="entry name" value="Fanconi anemia, complementation group M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1320.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039686">
        <property type="entry name" value="FANCM/Mph1-like_ID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044749">
        <property type="entry name" value="FANCM_DEXDc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14025">
        <property type="entry name" value="FANCONI ANEMIA GROUP M FANCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14025:SF20">
        <property type="entry name" value="FANCONI ANEMIA GROUP M PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="333"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="747"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="44"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="164"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="209"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="777"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="960"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="78"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="176"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="209"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="251"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="266"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="777"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="792"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="961"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="13">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ82069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1169" mass="130942" checksum="70253798F5497685" modified="2017-01-18" version="1">MNDHDLTDLSDLDDNDMALLLNKDQDHHRETDAINNDINEQNKNSAHHAMDSGTSQLGHKNGSILDTTXSASSGANKTLEKGLQEEPDWHISDDSDDDLLDLLTSKQSTGXNANTSSTSXKTXDTNIFNLASSYNRAPQNIDNDVTSIPKDGANTLTGNIFMIDQGSAGQNGHIPKSSTQLNSTSGISTGEHQNATEKTTREFAGANADIVNDDAAANADGDDDDDDGDDDDDDDDDDDDILNLASKPKAHNSQPIMSPDSSFPQNNSNSNLHSNNTSFGYEEPQLFDSGGDYGYQEINLIPTQTIIRPFNENNIHTFLYPTNYPVREYQLTIAKEAIFKNVLATIPTGTGKTFIASVVILNYYRWFCSLPDFEQNGKIIFMAPTRPLVAQIIKACLGITGIPYDETAILLDKSRKNRETIWEEKKVFFTTPQVVENDLKRGVLDPKKIVLLVVDEAHRAKGSYAYANVTKFLERFNKSFRVLALTATPSADIEGVQEVVTTLSISKIEVRTESSPDVEKYMKTKDVVQIDVPALNXDIVEIIDILSEAILPVLREANETDLYEETDPSRINAFLALQKANKVIANPSLPEGLKWKYYFILQLLSHVGQMLRRLKIYGIKCFYNYFTDKYKESNAKYSLGKSKNKTAASFYQNTKLLQIFTKCDEMLKDPNFITHYKFKYMVEELVEFFCDSNTNSGSSLNNDSRVIIFTELRESALEIVKCIDSLQNEKIKPHIFIGQARGKEXFDETEYLLKNKKKGHKKVERAALEREKSDYQLEKQMKKKHEKETRISSRTGSSEEAQVSGMNQTQQRQVINDFKAGVFNVLVCTSIGEEGLDIGEVDLIICFDATSSPIKNIQRMGRTGRKRDGKIILLLSGPEKGKFNRAMETYQQLQDSIVFGNSIVYQKSDRMLPPEIVGINKGLELVFSEIKINDTNAKVNEMDDPEEVIKFATQAMTGKLNAKKKSAKRSKRIKTAGGTKKQKQFHMPEXVTGGIISAIQMLQKRNPLNDRSSTEEQTPVIENPXDDLVDLSVGEAHEQCAAPEGPIAMQEPRAVASSTVGDXLADIMGSFDENSSDDDADIRRIVNNFGQPKASLELPKEQLAXTVVFKNDSQSRKNTQDLSLEEYFQKNYKPIETSMVINADLNKPHKPIRVKHTMFMQNVFKIFHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7B7</accession>
    <name>A0A1E5R7B7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BRO domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3310</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7B7">
        <property type="interactions" value="86"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7B7"/>
    <dbReference type="InParanoid" id="A0A1E5R7B7"/>
    <dbReference type="OrthoDB" id="2141925at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.560">
        <property type="entry name" value="alix/aip1 in complex with the ypdl late domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.50">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025304">
        <property type="entry name" value="ALIX_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030">
        <property type="entry name" value="PCD6 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030:SF30">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13949">
        <property type="entry name" value="ALIX_LYPXL_bnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03097">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01041">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51180">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRO1" evidence="8">
        <location>
            <begin position="4"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="725"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="563"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="725"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="9">
        <location>
            <position position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ82802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="830" mass="93596" checksum="0791B987605517DD" modified="2017-01-18" version="1">MNPLILSLNLKDTEPVHLDKALSSYLKSCYGHSQYKAFFDKPLALKFDNLRVTANSDLGDEPLLELNYKYVAMLEQLALRSGNNSSILKTEFTWHDARNDGSLPTSPVEKYTQKSLAIEKSSIIFNIAALLSKVGKEKITGGTQDYALAIKSFSKSYGCFKYLSENFLNSPSKDIKTATTKFLSNLMHAQAQELYSLKIINSPDPIKQASLLSKMAFSTHKLYAACEEFYGESPVTTSSVGVPSYGEAKWKDIIHFKQVFYKSLSAYYHAAVLENGSKIGEAIGFLELSKKTYLQALPLKLYVKDMDFEVYQDIIEKKLASLIKDNDYIYHDTIPAEISVDTIKAMDAIKPVSYMESQLAPYMSEVSGDSNVLFRGIVPIEVYEKASLYSEKKSSKFREENETAQTADWEYDSFVEYTNLAKVLPDLEGKYSGSGNSHTDPETEYIDQQISRWSKEVMKSPYNDIPKQQQDILKMRKRILDILSVVSDKENSVKLKSSLVQASQSDEKLFAVVSPYQQEIALLKNFSGLKSIFDSYKVDNEEVDLLGIDDSKNEMILAEIKKCKETFNDLRLLKRERDRLIDEMKQKLNNDESDEGVIQLILENQDSKDIGHLFDGLMEQHFGELSSRIEATIFKQKNLITEIKIQLDSIFKSTGVHKKSGDMQEKLKQRNEMFSKIEEAYNCFKIFANDLPKGINFYSSLLTMVNTLKERESQSVNVTHQFGSLSMENGSGNASDLQSKATPPMVPSKPAAGFGLNYESSTAPKIPQRPNELESKPTFGVAQNYSRPQLPLKPSSNASDIXKGNGYQGNPTAFYNNPSVFDESLYNSFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7D6</accession>
    <name>A0A1E5R7D6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Dityrosine transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3319</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7D6">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7D6"/>
    <dbReference type="InParanoid" id="A0A1E5R7D6"/>
    <dbReference type="OrthoDB" id="3066029at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005275">
        <property type="term" value="F:amine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF21">
        <property type="entry name" value="DITYROSINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="305"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="334"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="456"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="493"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="536"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="561"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="627"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="180"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="98"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ82806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="671" mass="73621" checksum="9FC3C3E7E9D9CAB7" modified="2017-01-18" version="1">MLLSNGSADDESKSKHKLNKQQMMHNLRDTSKSSNMIVSDTKNVCAAQTVDDLNNNNNTSCRDNDNESNIGNEHLDVISSTSSCVVKQLQAKINDCKETTENGKDPDNKKVKSANEKPNKKKSSFADIATGKSSVNSLTVKKDISPPCVTEDTGQELSTFQGPQPPYLYSVYSKEKKVCIFLIITFCGFLGPLAGNIFIPALPFLQTVFAVDDTTINATVSVFMAVFSVGPIIWGMYADSYGRKKLYIASVGIALVANVLLSVVPAKISALYVLRIVQAFGTSGVIPLGLGTVRDIVDISHRGRAVSVFMIGPNAGPVLAPIIAGLILKNGDYSWRWLFGTLSIMTGVAFVAVLWFLPETLRSLVGNGDKDWESKSMVDVNKKSNTVDGKKVEHLENTNILVQEKNDHHEKRDKLKKKLFGVKNPVNDSEAFLEMYPKAPKPHLKTFFNMIKDXRILLNSFCNAIVFAVYYGFSVTFAYRLKEEYKLNNLQVGAAYCCPGVALIIGTLSSGYFSDRVLNHFKENSKNGIYAKENRLILQLFGLLVSCCGSVGYGWSIEKKVNLVIVLVFAAFSAFGMTWCSNASITYMIDVKGRQAATAISINNSFRNAAAAISAAIINKLTRQMGFGWCFTGLGVCVFLAEILLIFLIAKGNQELREKVDLECAPEKESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7S4</accession>
    <name>A0A1E5R7S4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">T-complex protein 1 subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">CCT-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3180</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7S4"/>
    <dbReference type="FunCoup" id="A0A1E5R7S4">
        <property type="interactions" value="1379"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7S4"/>
    <dbReference type="InParanoid" id="A0A1E5R7S4"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03335">
        <property type="entry name" value="TCP1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000009">
        <property type="entry name" value="T-complex protein 1 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000070">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012715">
        <property type="entry name" value="Chap_CCT_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02340">
        <property type="entry name" value="chap_CCT_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ82938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="561" mass="61153" checksum="03426AE44D3B7D0D" modified="2017-01-18" version="1">MFSSSRSDTLFLGGEKISGDDVRNQNVLSAMAVANVVKSSLGPVGLDKMLVDDIGDFTVTNDGATILSLLDVQHPAGKILVELAQQQDREIGDGTTSVVIIASELLKRANELVNNKIHPTTIITGYRVALREAVRYINEVLSCSVESLGKQTMVNIAKTSMSSKIIGSDSEFFSNMVVDALLAVKTLNGRGEVKYPVKAVNILKAHGKSSKESVLVDGYAINCTIASQAMPTRVGNSEQVDSQVKIAVLDMNLQKTRMAMGVQINIEDPEQLEKIREREYGIILERIRKIIDSGAQVVFTTKGIDDLCLKEFVDKKIMAVRRCKKEDLKRIARATGATLITSMSNLEGDETFESSYLGTAKEVVQTRFSDDECILVKGTAKHSSSSIVLRGANDYSLDEMERSLHDSLSVVKRTLESGMVVPGGGCVETALNIYLDNFATSVGSREQLAIAEFAQALLVIPKTLAINAAKDSSDLIAKLRSYHAASQSCPLDDLKRRKYKNYGLDLIRGKIVDEVKEGVLEPTISKIKSLKSALEACIAILRIDTMITVDPEPEKQDPHGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7W5</accession>
    <name>A0A1E5R7W5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">tRNA(Thr) (Cytosine(32)-N(3))-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3410</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. METL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7W5"/>
    <dbReference type="FunCoup" id="A0A1E5R7W5">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7W5"/>
    <dbReference type="InParanoid" id="A0A1E5R7W5"/>
    <dbReference type="OrthoDB" id="417697at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052735">
        <property type="term" value="F:tRNA (cytidine-3-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000221">
        <property type="entry name" value="Methyltransferase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013217">
        <property type="entry name" value="Methyltransf_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026113">
        <property type="entry name" value="METTL2/6/8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22809">
        <property type="entry name" value="METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22809:SF11">
        <property type="entry name" value="TRNA N(3)-METHYLCYTIDINE METHYLTRANSFERASE METTL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08242">
        <property type="entry name" value="Methyltransf_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 12" evidence="5">
        <location>
            <begin position="385"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="156"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="183"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="216"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="231"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ82999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="574" mass="65545" checksum="A1DB9CB8DCC3C3D3" modified="2017-01-18" version="1">MAVADLIKKFETFSHKSGTSGESEDDAHSNSVHASPKKRTIDPKVFVDGTAKNDKEHTQILKDLSTGDSAETGNSEDTETNITFDEEKPTEMELEENSTEEPAQTQFEQSLTTDAPSSSNENNAATVNEKKQETPTPDQAQEKLDTLGSAVDEEATEKLFESVKVEEAEAELETSPMEVELEGVEKPKELESHIDTAIASSSTNEELPEENKDAQQDDQDKEEGEDPSGNETHEAFEENKTTVGTGVHNPENISVLNVDEEEDYTNAQNHKDIKPPLDTRLGRDDPFQFGTRYLNDETKIWDYNSWDHFEFGDEDIQNAQEKIKNQTFVPEEDRAKYYENPARYWDIFYKNNKENFFKDRKWLQIEFPTLYSATQKDSGAINICEIGCGAGNTFFPILRENENEKLRISAVDFSPRAVELVKTSPDFDSEYGHAAVWDLANEEGDLPEGLEPHSVDIAVMIFVFSALAPKQWSQAMDNLHKLLKPGGKILFRDYGRYDLAQVRFKKNRLLDENFYIRGDGTRVYFFTEEELRSIFTEKYFTENKIGTDRRLLINRKKQLKMYRQWVQAVFDVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R813</accession>
    <name>A0A1E5R813_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vacuolar protein sorting-associated protein 28</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ESCRT-I complex subunit VPS28</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3276</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the ESCRT-I complex (endosomal sorting complex required for transport I), a regulator of vesicular trafficking process.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the VPS28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R813"/>
    <dbReference type="FunCoup" id="A0A1E5R813">
        <property type="interactions" value="592"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R813"/>
    <dbReference type="InParanoid" id="A0A1E5R813"/>
    <dbReference type="OrthoDB" id="3970451at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037202">
        <property type="entry name" value="ESCRT_assembly_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007143">
        <property type="entry name" value="Vps28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017899">
        <property type="entry name" value="VPS28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037206">
        <property type="entry name" value="VPS28_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017898">
        <property type="entry name" value="VPS28_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038358">
        <property type="entry name" value="VPS28_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12937">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING 28, ISOFORM 2 VPS28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12937:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 28 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03997">
        <property type="entry name" value="VPS28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017535">
        <property type="entry name" value="VPS28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140111">
        <property type="entry name" value="Endosomal sorting complex assembly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140427">
        <property type="entry name" value="VPS28 C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51310">
        <property type="entry name" value="VPS28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51313">
        <property type="entry name" value="VPS28_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0967">Endosome</keyword>
    <keyword evidence="4 5 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VPS28 N-terminal" evidence="9">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="VPS28 C-terminal" evidence="8">
        <location>
            <begin position="159"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="124"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR017535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ83050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="262" mass="29556" checksum="63457F5C13ED0C07" modified="2017-01-18" version="1">MYTKQQQLEYSLKDNITKDEEELMDGLAEIFSIVVTIDKLEKLYLKDCIDDDVYTDQAMKMMAQYKSICTDNARVTQCYHEDLDIFVQMYSLMNCSNGVQRLKKGIPATVEHFQLKSGDGSLVPSGNLNNASDSQAGNGDTTSIGGSSQHQVHSQNTMLDGKAVAETTGNFITLMDALKLKYNTKDQLHPLMSALLLSVNKVFNSSPLLKNRISLFKERQQLVEWVIKLNHLKVGQKLSENEIKDMLFDLDQAYKGFYSLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R873</accession>
    <name>A0A1E5R873_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">26S proteasome regulatory subunit RPN8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3456</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R873"/>
    <dbReference type="FunCoup" id="A0A1E5R873">
        <property type="interactions" value="1089"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R873"/>
    <dbReference type="InParanoid" id="A0A1E5R873"/>
    <dbReference type="OrthoDB" id="10256771at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024969">
        <property type="entry name" value="EIF3F/CSN6-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540:SF7">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13012">
        <property type="entry name" value="MitMem_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MPN" evidence="2">
        <location>
            <begin position="9"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ83088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="358" mass="40128" checksum="8FAB332C2ECB9039" modified="2017-01-18" version="1">MIPVEEKTVSVAPLVLLSALDHYERLNTQGTKRVVGCILGENSPNSKTIKVTNSFAIPFEEDVKNSDVWFLDQHYIEQMLQMTKKISAKEKLLGWYHSGPKLKSNDLKINNSIFKKYCNSQPILLVIDVKQQVSVGLPTHAYVAIEQVKDDGTSTENVFNHLPSIIEAEEAEEIGVEHLLRDVRDQAAGGLSIKITNQLKSLKGLQRKLEDIIKYLQKVANKELPVNHTILGKLQDVFNLLPSLGSSNADSLIVGQDTQSDRASNEQASSNITTLNKALTVKTNDELMIMYISQLVRSIIAFDDLIENKIQNAKLQKQYHEIESSSTTKESEKEKEKASETKENKEGKTEKKDPAKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R877</accession>
    <name>A0A1E5R877_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Polynucleotide 5'-hydroxyl-kinase GRC3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Polynucleotide 5'-hydroxyl-kinase grc3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3437</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R877">
        <property type="interactions" value="829"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R877"/>
    <dbReference type="InParanoid" id="A0A1E5R877"/>
    <dbReference type="OrthoDB" id="3972306at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051731">
        <property type="term" value="F:polynucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1030">
        <property type="entry name" value="Clp1, DNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.330">
        <property type="entry name" value="Pre-mRNA cleavage complex subunit Clp1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045116">
        <property type="entry name" value="Clp1/Grc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010655">
        <property type="entry name" value="Clp1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038238">
        <property type="entry name" value="Clp1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032324">
        <property type="entry name" value="Clp1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038239">
        <property type="entry name" value="Clp1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032319">
        <property type="entry name" value="CLP1_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755">
        <property type="entry name" value="CLEAVAGE/POLYADENYLATION FACTOR IA SUBUNIT CLP1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755:SF6">
        <property type="entry name" value="POLYRIBONUCLEOTIDE 5'-HYDROXYL-KINASE CLP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06807">
        <property type="entry name" value="Clp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16573">
        <property type="entry name" value="CLP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16575">
        <property type="entry name" value="CLP1_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Clp1 N-terminal" evidence="6">
        <location>
            <begin position="35"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Clp1 P-loop" evidence="7">
        <location>
            <begin position="136"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Clp1 C-terminal" evidence="5">
        <location>
            <begin position="349"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ83112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="460" mass="51832" checksum="54548B84A24B79B6" modified="2017-01-18" version="1">MSNVGNGLDADEEDANLAQENVDFLDFDVVHTVSLLQGYEWNIELKPEQSLRLRVSSGVAEIFGVELANEASYSFNSQENIAIFSVENCEIEWQSDKNLEISTNKNNYKLKLYSLHFALDKIRSTYLQGPNVVVVGKKNTGKSTMCKTLLSYALKFRDFNPTFINLNSAEGIFTMPGCLTATTVTDLFQAENPQWNESASTTVNSMIPTVQPLVKLFGYESISGNYPLYLLTIQNLSSVVNRKFEKMVELQRSGMIVDTPPLDFETNEFDNEILRVIIESFKITTIVELVDSVTGEEQQSQFLRKTYPQCTIVTLPKDECVVEKPDSYDRFLQRNAIKDYFYGNANNLLSPYTISIKFEDLVVWKPKEYTSVQEAISDQAGVLEMKKVSVSQSTLQHAVLAITFADXLESNPHKIMNSGVMGFGLVTEVSDAKSRLKLLIPIPGQLPRNALVLTQYRYLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R8F6</accession>
    <name>A0A1E5R8F6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Stress-associated endoplasmic reticulum protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3286</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Interacts with target proteins during translocation into the lumen of the endoplasmic reticulum. Protects unfolded target proteins against degradation and facilitate correct glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum membrane</location>
            <topology evidence="6">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the RAMP4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R8F6"/>
    <dbReference type="FunCoup" id="A0A1E5R8F6">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R8F6"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010580">
        <property type="entry name" value="ER_stress-assoc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06624">
        <property type="entry name" value="RAMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ82813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="67" mass="7756" checksum="3BA31E741BC9E2A8" modified="2017-01-18" version="1">MAIQTPKQRQANQKFNKNIEKNRKFGQPKLNKKDLENNKLKLSRTWLIVLGFLLVGGGLLEIVSLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA59</accession>
    <name>A0A1E5RA59_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Adenylate kinase isoenzyme 6 homolog</fullName>
            <shortName evidence="10">AK6</shortName>
            <ecNumber evidence="10">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Dual activity adenylate kinase/ATPase</fullName>
            <shortName evidence="10">AK/ATPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2953</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Has also ATPase activity. Involved in the late cytoplasmic maturation steps of the 40S ribosomal particles, specifically 18S rRNA maturation. While NMP activity is not required for ribosome maturation, ATPase activity is. Associates transiently with small ribosomal subunit protein uS11. ATP hydrolysis breaks the interaction with uS11. May temporarily remove uS11 from the ribosome to enable a conformational change of the ribosomal RNA that is needed for the final maturation step of the small ribosomal subunit. Its NMP activity may have a role in nuclear energy homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with small ribosomal subunit protein uS11. Not a structural component of 43S pre-ribosomes, but transiently interacts with them by binding to uS11.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the adenylate kinase family. AK6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="10"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA59">
        <property type="interactions" value="839"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA59"/>
    <dbReference type="InParanoid" id="A0A1E5RA59"/>
    <dbReference type="OrthoDB" id="10251185at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000372">
        <property type="entry name" value="Adenylate kinase isoenzyme 6 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00039">
        <property type="entry name" value="Adenylate_kinase_AK6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020618">
        <property type="entry name" value="Adenyl_kinase_AK6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595:SF0">
        <property type="entry name" value="ADENYLATE KINASE ISOENZYME 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595">
        <property type="entry name" value="POS9-ACTIVATING FACTOR FAP7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13238">
        <property type="entry name" value="AAA_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 10" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="NMPbind" evidence="10">
        <location>
            <begin position="69"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="10">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="236"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="241"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ83795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="292" mass="33473" checksum="83B00460303F6034" modified="2017-01-18" version="1">MGKSTTKXNDITDNTRRYFPNIIVTGTPGCGKTSTSELLQRSLNALLQEQKQEGKSSKQXTIPQYDLYNISEYAKREKLFEXFDEVRNSHIIDEDALLDNLEPXLRRGGNIIDWHCNDIFPERLIDLVVVLRCENAGLIERLTXRKYSDFKLNENLDAEIMGVVLQDALDSYVQDIVIELQSNNVNDMETNSDRISSWVEMWCKQHKEKGVTNEVEEFYQDRDDVDFKAGVVIDNGSFEFDXQDSDAWDDDGEEEEEGEEGEEGEEGEEEEEGEEGEEGEEGEEETDNDSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAH5</accession>
    <name>A0A1E5RAH5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Ergosterol biosynthetic protein 28</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g2770</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERG28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAH5"/>
    <dbReference type="FunCoup" id="A0A1E5RAH5">
        <property type="interactions" value="265"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAH5"/>
    <dbReference type="InParanoid" id="A0A1E5RAH5"/>
    <dbReference type="OrthoDB" id="6485510at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005352">
        <property type="entry name" value="Erg28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15451:SF19">
        <property type="entry name" value="ERGOSTEROL BIOSYNTHETIC PROTEIN 28 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15451">
        <property type="entry name" value="ERGOSTEROL BIOSYNTHETIC PROTEIN 28-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03694">
        <property type="entry name" value="Erg28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="8" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="11" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ83909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="144" mass="16473" checksum="3B8B9F506B2069BE" modified="2017-01-18" version="1">MFDCCISLVQRVLAAPGYLPKWLFFISVVSIFNSVQCYVSGIKLTQKVYSKAPSKINSLSCRLFGTWTFVACIIRLYGSLFYYEEHIYQLTFISYVVALLHFGSELVLFRSCALDKGFMGPLIVSTTSLAWMYLQKEFYTGSSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAM2</accession>
    <name>A0A1E5RAM2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2577</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RAM2"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="651" mass="74556" checksum="C2C7F0AF10C0A9DC" modified="2017-01-18" version="1">MNTHNSTNNSLSVPKITYLSPXYRQQNHRLKKNGVHKRKKQNEKLCSTKYLLGYIPVLADSYQNSSSXKXQPTPQEKHFKQKSQTRLSKLDANTKSNGQSTPEITPLGKFHNETKSGVRRLLTFALANVPKLLFPAGELSNKNNEKKFITEYIYTLXSLCVGAANDYKDTXIAGFEEAEQFLLQSYTYTETXNGYIVYFVLNPSIPKPILKMCLFRMFSMFGQRNKTNSNFEKPGISLKCNEMHYAFFKEELGVDVLNIKDDEKYESARVEMFKRIDEYKKDTIKNFNNELDGSSTSGKNSSIGNSPSLFDIDYNMLKDVPQSELSLVVENIHAFRNDVLIKEQDKLQKEKLXELARQREKLKKVYKNMVHADRSEEGVVELDQKEEEEEEEEEEEEEEEEEEXDDEYEDEDEIDNDEQLEEQRVQETARAAEAQYETLLDKSTNSPXGLLYKYKQLLMSKDDDLEKYKDDSQDTELLKKLNQYCAEHIKNNTNIFNKNDIIEQRRLDDLNRVKFGTPAELDVDNEVTLETATATSTLATEITTPSTSGIKISLSSTAPTSTISHLEKLLSSLLSKHLADSSTEEDNQEVIGIIMDIIKDNSYNYDLYHEELGIILGDLFEEEQSQDSSNEDSTSANVVQDILAALKNELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB38</accession>
    <name>A0A1E5RB38_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carboxymuconolactone decarboxylase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3083</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RB38"/>
    <dbReference type="FunCoup" id="A0A1E5RB38">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB38"/>
    <dbReference type="InParanoid" id="A0A1E5RB38"/>
    <dbReference type="OrthoDB" id="5537330at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052999">
        <property type="entry name" value="PTS1_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28180">
        <property type="entry name" value="CONSERVED MITOCHONDRIAL PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28180:SF2">
        <property type="entry name" value="PEROXISOMAL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="272" mass="31228" checksum="DBB92E2057026AAF" modified="2017-01-18" version="1">MSVLTRSRLLYIQNSIPLQQRNNLQLSYIWYLSAAATLSICNQPQDVKTVYKYALLQDMDKSLDDDFQIKHFTPLQQHMTRKFKEALLKSSCIGGLPKAINSLHSVASAAATKEQDVGEEEEEEEEEEKDKEEDKFRFRSESPNFVRKSDYESESKEVILKRGNEHFRRLYSKVSDKIITKMNGTSPDLWYTVLSCCYGPILSNEEVLNDQESSIVVISSLIPQNVNPQLYGHLKGALNIDCDKNFINSVQNLSLEISEWCGIYLKEKSVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB88</accession>
    <name>A0A1E5RB88_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Meiotic nuclear division protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2829</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB88">
        <property type="interactions" value="190"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB88"/>
    <dbReference type="InParanoid" id="A0A1E5RB88"/>
    <dbReference type="OrthoDB" id="9978204at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040453">
        <property type="entry name" value="Mnd1_HTH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03962">
        <property type="entry name" value="Mnd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mnd1 HTH" evidence="3">
        <location>
            <begin position="36"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="106"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ83833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="223" mass="25722" checksum="F6B0D7CDED4304ED" modified="2017-01-18" version="1">MVSFVGKDLTTKPGKQTQTKKRKVVTVEEKCLIFQKILHAEHEIYSLKELETIIPRKSNGAIHSMVIKELIEHMSNEDMINVEKCGNVNVYYSFPFTQIQKDETLYQNILTKHENLKKSITELETKINFINSNKSAELNRLNKELKAEIKKDDALTSKITQSEQKLKDNNTNDLQXILNEQIGKTALLKNNIDVILDYVLQKFPHIARKELLTEIGLPHDXLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBB4</accession>
    <name>A0A1E5RBB4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cell division control protein 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2718</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBB4">
        <property type="interactions" value="176"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBB4"/>
    <dbReference type="InParanoid" id="A0A1E5RBB4"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032161">
        <property type="term" value="C:cleavage apparatus septin structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031105">
        <property type="term" value="C:septin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005940">
        <property type="term" value="C:septin ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000162">
        <property type="entry name" value="septin-7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="9">
        <location>
            <begin position="23"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="367"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ84190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="414" mass="47480" checksum="4A9366219610B4BE" modified="2017-01-18" version="1">MVYDSEIGISELPNQVYKHVTKQGSQFNLMVCGESGLGKSTFINTLFQTNLKPLVGQYPRTPNKNNLNKTTEIEIIRCLLEEKNFSLKVNVIDTPGFGDNVNNNKTWSTIVDFIDDQHDSFMRQEQQPFRNEKFDMRVHAVLYFIKPTAQGLKPLDIVTMKEISSRANLIPVIAKSDTLTKEELKIFKIRIRQVIEAQEISIFTPPLHIEETGSTTEKSGVNPAPQDSVAAAAIEHARQLIDAMPFAVVGSEEQYTVEGNLVYGRKYPWGIVEVENDXHCDFRKLRSLLLRTNLLDLILSTQESHYESFRRVRLGGVNNENTNLKSENGVENKNVSVPIPAPTRKLSHNPKFKEEELALKRYFTDQVKAEEQRFRQWEQNIVNERIRLNSDLEDVQNKVKKLEDQVRNLQLQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBK5</accession>
    <name>A0A1E5RBK5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Uridylate kinase</fullName>
            <shortName evidence="9">UK</shortName>
            <ecNumber evidence="9">2.7.4.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP:UMP phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Deoxycytidylate kinase</fullName>
            <shortName evidence="9">CK</shortName>
            <shortName evidence="9">dCMP kinase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Uridine monophosphate kinase</fullName>
            <shortName evidence="9">UMP kinase</shortName>
            <shortName evidence="9">UMPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">URA6</name>
        <name evidence="11" type="ORF">AWRI3579_g2463</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. Plays an important role in de novo pyrimidine nucleotide biosynthesis. Has preference for UMP and dUMP as phosphate acceptors, but can also use CMP, dCMP and AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>UMP + ATP = UDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:24400"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Binds 1 Mg(2+) ion per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
        <text evidence="9">Predominantly cytoplasmic.</text>
    </comment>
    <comment type="domain">
        <text evidence="9">Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the adenylate kinase family. UMP-CMP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.14" evidence="9"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBK5"/>
    <dbReference type="FunCoup" id="A0A1E5RBK5">
        <property type="interactions" value="654"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBK5"/>
    <dbReference type="InParanoid" id="A0A1E5RBK5"/>
    <dbReference type="OrthoDB" id="442176at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033862">
        <property type="term" value="F:UMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000315">
        <property type="entry name" value="Adenylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03172">
        <property type="entry name" value="Adenylate_kinase_UMP_CMP_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006266">
        <property type="entry name" value="UMP_CMP_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01359">
        <property type="entry name" value="UMP_CMP_kin_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9 10" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="NMPbind" evidence="9">
        <location>
            <begin position="42"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="9">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="22"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="70"/>
            <end position="72"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="105"/>
            <end position="108"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ84261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="205" mass="23323" checksum="42E02FAABBF02626" modified="2017-01-18" version="1">MSIDSKPVPKDISVIFVLGGPGVGKGTQCARMVKDYGFVHLSAGDLLRAEQNRKGSEFGELIKKYIAEGLIVPQEITIQLLKNAILEHYYTKETSKQSKKFLVDGFPRKMDQAITFEKEITPSKVVLFFDCSEDIMLTRLMERAKTSGREDDNIESIKKRFKTFVETSMPVVEYFEKMDKVVKIDCAKSVDEVYEEVKAALKNYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBM0</accession>
    <name>A0A1E5RBM0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aspartate aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2881</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBM0"/>
    <dbReference type="InParanoid" id="A0A1E5RBM0"/>
    <dbReference type="OrthoDB" id="7042322at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43510">
        <property type="entry name" value="AMINOTRANSFERASE FUNCTION, HYPOTHETICAL (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43510:SF1">
        <property type="entry name" value="AMINOTRANSFERASE FUNCTION, HYPOTHETICAL (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="1">
        <location>
            <begin position="47"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="382" mass="43281" checksum="77B948CE21F94CAB" modified="2017-01-18" version="1">MPYQEKFEVEDFMDEYETLIEYNLGETCCYSLSLNEVEKISGEKFEFDFDTRFTYGVIDGTEPLRELIAGLYSNQDVKFTKDNVLLTNGAIGANFLTYYTLFGKGDHVICVSPTYQQLFSVPKMFGADVELLXLXKENGFVPSLDEFAKLIRNNTKAIILNNPNNPLGTVIDTASLQQIEEMAERHGITVICDEVYRPLFHSIEQTPKSIVQLSSKGISTGSMSKAFAFAGVRLGWIVTQDLEFLKAAKSKRHYNTISVSIADDQISQYVLRNKEPVLQRNNQLCLTNLAYLKQWLSKNNFAEFYNVPQGGSVCLLKFKGISDTYHFSCWLAKSKRVLLVPGETFECPGTVRLGYANSYEELVKGLDILSASMDEYMKEFKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBM3</accession>
    <name>A0A1E5RBM3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">2-dehydropantoate 2-reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2884</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBM3"/>
    <dbReference type="FunCoup" id="A0A1E5RBM3">
        <property type="interactions" value="43"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBM3"/>
    <dbReference type="InParanoid" id="A0A1E5RBM3"/>
    <dbReference type="OrthoDB" id="3609at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000017">
        <property type="entry name" value="2-dehydropantoate 2-reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051402">
        <property type="entry name" value="KPR-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013332">
        <property type="entry name" value="KPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708:SF30">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708">
        <property type="entry name" value="PROBABLE 2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02558">
        <property type="entry name" value="ApbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketopantoate reductase N-terminal" evidence="1">
        <location>
            <begin position="9"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Ketopantoate reductase C-terminal" evidence="2">
        <location>
            <begin position="222"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="370" mass="41189" checksum="0E5AB629641119D7" modified="2017-01-18" version="1">MSTQTPQCLIIGAGGVGVVTAYSLFLNKGSTVSLVARSDYDKASTTGYKLSSCDYGEVLNWKPHHLFRSVEDAASSKTFFDYVVVTTKNIPDGPTPVSSIIKPIIESNDKVWKERADFKEQKKTTNVVLIQNGIDIENEIKEKFDLASRQKSTTVPQVALISGIQLISSTKVGPCEIAQKGHDHLTCAAFDKNDEISVKTAQNFISLYTNPGQNQAHFDSNCRYTRWKKLLYNSSINTLTAICGLDFTRCLQFGTAEGSTEFEIVRPAMLEIIDLAKKIDDITIEVDLVDFFVDITRKMVYTPSMGVDIQKGQLMEIEVIVGNVVRSAKAHHIEVPHLSMLYNILKILQGKLKEQRGLLKFDELNAKIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBR0</accession>
    <name>A0A1E5RBR0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Growth regulation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBR0">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBR0"/>
    <dbReference type="InParanoid" id="A0A1E5RBR0"/>
    <dbReference type="OrthoDB" id="9451547at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="445"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="445"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="610" mass="68544" checksum="5F0C8C5857CDEDCF" modified="2017-01-18" version="1">MPSQGMQGNGNINPNSILTQVSGDSQFLKANLSSAVGKNGKVDPYSSPSDPVYPPSKYSSKRHQSQTNTASSSSMISHHSVNDHSINNSGDHVSQSHLNNNNNIYVDPNAKYYFDVRTYDRNVDSLIIHLNIEEHHYYITRDQLLSLPESLLLCLFPNGVFMDRLGDVIHTLTEEDEVYVFNFSYECFEYIMEVYSQATYDMSHYPLSKMLELYGDPVAVAAAATASNNHGHHGASGFFSQLAHHASASASSLTSSQKTAAQAAANSEASFLQKNPSIIVLREDLDYYCIPLSIKIEPSNHEVLDVFMRSVKIAAGGYLRDQMSIFDGLYCSNKVEINKETGEKKLGVAEQHLMDMLCTSGLPLNSXWGNRSQEHDKTVISSLSLIRLQNETTSEFRDKVAKSYTKHLEEDKVKIEQLKVLQHQQQQQQQQQQQMQLSPQISRDSLADNLNSPHSHSATPHNNHLNSASGNELTPTSSRKSRWSTKLSSVRSRSHSRNASKHRSESSNNIEAQISKVGPKSRHLYDYAPKPEINSKLLLFWKKPARKCWWGNLECVLDIELPFEVEYHKDEKDGQVTILKVKGPGYTKLSLPVRLHIRRVWTLEVSIVGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBR4</accession>
    <name>A0A1E5RBR4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ATP-dependent RNA helicase DBP5</fullName>
            <ecNumber evidence="4">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">ATP-dependent RNA helicase dbp5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">AWRI3579_g2529</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the DEAD box helicase family. DDX19/DBP5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBR4">
        <property type="interactions" value="795"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBR4"/>
    <dbReference type="InParanoid" id="A0A1E5RBR4"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17963">
        <property type="entry name" value="DEADc_DDX19_DDX25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000849">
        <property type="entry name" value="ATP-dependent RNA helicase DBP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000318">
        <property type="entry name" value="ATP-dependent RNA helicase DDX19B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 19 24" id="KW-0347">Helicase</keyword>
    <keyword evidence="7 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="12" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="6 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="11" id="KW-0811">Translocation</keyword>
    <keyword evidence="12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="23">
        <location>
            <begin position="80"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="21">
        <location>
            <begin position="113"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="22">
        <location>
            <begin position="293"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="18">
        <location>
            <begin position="80"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00039604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="OEJ84337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="475" mass="53001" checksum="FF19049BC9B650BE" modified="2017-01-18" version="1">MSAKPATPNSTATNGSSKDPSELMSNLKLQSSKDEQAASTKDSTEKPKIQDSNLVKSEYEVKVNLADIQADPNSPLYSVKSFEELGLKPELLKGLYAMKYTKPSKIQEHALPLLLKDPPKNMIAQSQSGTGKTAAFCLTMLSRVDEKLVGVPQCICLAPARELARQTLEVLQEMGKYTNISSKLVVPESYERNKKIDAQIIVGTPGTVLDLSRRXLIDVSRIKVFVLDEADNMLDAQGLGDQCLRMKKFLPAANCQLVLFSATFDDRVKQYAKKVVPNANSLELQRNEVNVSAIKQLYMDCQNEEQKFEYLTELYGLLTIGSSIIFVAKKETANELYYRLQKEGHKVSILHGDLKVDDRDRLIDDFRSGKSKVLITTNVLARGIDIPTVSMVVNYDLPTLPKSNGSQADPATYIHRIGRTGRFGRTGVAISFVHDKKSFTVLQQIKQYFGDIEMTKIPSDDWDEVEKIVKKVLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBX8</accession>
    <name>A0A1E5RBX8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Mitochondrial chaperone BCS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g2685</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family. BCS1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBX8"/>
    <dbReference type="FunCoup" id="A0A1E5RBX8">
        <property type="interactions" value="1107"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBX8"/>
    <dbReference type="InParanoid" id="A0A1E5RBX8"/>
    <dbReference type="OrthoDB" id="10251412at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19510">
        <property type="entry name" value="RecA-like_BCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000768">
        <property type="entry name" value="Probable mitochondrial chaperone bcs1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057495">
        <property type="entry name" value="AAA_lid_BCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014851">
        <property type="entry name" value="BCS1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050747">
        <property type="entry name" value="Mitochondrial_chaperone_BCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23070">
        <property type="entry name" value="BCS1 AAA-TYPE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25426">
        <property type="entry name" value="AAA_lid_BCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08740">
        <property type="entry name" value="BCS1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01024">
        <property type="entry name" value="BCS1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="BCS1 N-terminal" evidence="15">
        <location>
            <begin position="51"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="14">
        <location>
            <begin position="251"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ84391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="449" mass="50068" checksum="55BAC635E26379FD" modified="2017-01-18" version="1">MSLEPPKGPAGTPNLDMDKITESGSISGAANSLINQVFKENPYFAAGGGLMILGSALALARTGIKSGSSLLYRQLVVDLEIPSKDKSYLWFLEWMGKYPQRSSRHLSVETNFIQHDNGSVTTKFQLVPGPGSHLIRYKGAFMLIKRERSGKMIDMTNGAPFETVTLTTLYRDRHLFNELLLEAKKMATKQNEGKTVIYTSWGPEWRPFGQPKAKRVLPSVVLDKGIAKGILDDVKDFMQNGEWYLQRGIPYRRGYLLHGPPGSGKTSFIQALAGHLDYNICILNLAESNLTDDRLNHLMNNIPERSILLLEDVDAAFNKRGQTDESGFKSGVTFSGLLNSLDGVTSSEETITFMTTNHPEKLDPAIMRPGRIDYKQYIGNATPYQIEKMFMKFYPGESELCAEFVQKAEGLGLDISTAQLQGLFVFNKNDPQNALAMIETLRHQNHVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RCU1</accession>
    <name>A0A1E5RCU1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Mitochondrial ornithine transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2680</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RCU1"/>
    <dbReference type="FunCoup" id="A0A1E5RCU1">
        <property type="interactions" value="89"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RCU1"/>
    <dbReference type="InParanoid" id="A0A1E5RCU1"/>
    <dbReference type="OrthoDB" id="2139348at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990575">
        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF31">
        <property type="entry name" value="MITOCHONDRIAL ORNITHINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="2"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="93"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="191"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ84393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="284" mass="31073" checksum="DF0E3F05535FFD9D" modified="2017-01-18" version="1">MDDIICGSAAGALGKLIEYPFDTVKVRLQTSSHDVFPDTLSVIKYTVQKEGLITGFYKGVGSPLVGAMLENAVLFVAYNQTYKILDQKMQSLNELSKIVLSGGAAGSCASFVLTPVELVKCKLQVSNIQNPNNTNTILSTIKDVVKGYGVKGLWKGQSSTFYRECIGGCMWFTTYEVLKTHLASITQDKKTKTWHSLFSGAMAGIAFNASTFPIDTVKSLVQIDHHMTITQAIKQTYAKKRGIQGFYRGLGITLIRAAPANAVVFYAYETFSSLFFSKNLPPTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDH6</accession>
    <name>A0A1E5RDH6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcription initiation factor TFIID subunit 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDH6">
        <property type="interactions" value="285"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDH6"/>
    <dbReference type="InParanoid" id="A0A1E5RDH6"/>
    <dbReference type="OrthoDB" id="5402929at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00076">
        <property type="entry name" value="HFD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="389" mass="44190" checksum="ED6F680653129418" modified="2017-01-18" version="1">MNNQENFHFNLLRVSMIQLLSGVGFERANKQTLNTVTDLYIKFFSLLVKEVEKMVSIRDQQLSDDHNYSHNGSLLTLQDLSQALINLKIVKPYDTLDVYGENPYIADDAGMKKFKEWCGESTVLHESRLISRPDATQFMEEAVKSIEAVTRTDALNVKQDSGSLGLLKELQDDSAVPDDWIKVILKKQQLLLWKETRLSALTNDQSTPSSSHEQRLPTLPDSCITVPSISKQDYVSSKSNEAFPSTNENLSTELLPISHIQNRLDTIELSFENEELYEKMRKQHETNLADEKQRMKDLDDEKNEAAESNKKSNTTQTDPSNADGTYGNANETKNXSASQELLPYNGAAQPDMLLGLDQHPIEFEDFGDMDGAFQRRDSFTDHYDYDSNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDM0</accession>
    <name>A0A1E5RDM0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">ATP-dependent helicase ULS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g1792</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDM0"/>
    <dbReference type="FunCoup" id="A0A1E5RDM0">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDM0"/>
    <dbReference type="InParanoid" id="A0A1E5RDM0"/>
    <dbReference type="OrthoDB" id="423559at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF16">
        <property type="entry name" value="ATP-DEPENDENT HELICASE ULS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="559"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="943"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="1057"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="154"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="381"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ84976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1233" mass="140600" checksum="6ACE5A6261853654" modified="2017-01-18" version="1">MNMITTPRDEDGDIYILDSPPPPPPQTSAHLGESESTKFVPSQPFQRTIMTPNKSFPSSEFFRNTQTTNTATHDNNPHFNNHMDKVLTKNRANSEKTQKTPVIDFTKDDPDLLGTSTGSFTHMNSPVNELQPRVYDVDNESFVDAPISANANLSSLSSTSPSLSASASSSSSFVPLSTTTKQEHDEALALQQNLSRINQKFKKYDDLRAVNTTKSKVLQIKISKKMAKIKEYQREMDSIPPSNDSTRNQEIRAHFDAKIKFEEEKLKTNQQKLETTNKNLGLIQNGLNTLINQKTDFKLKLDKLKSGLNHTPSEMAALKNNTNNLNNLSVNADQQKRAAQEQLLQHHLKLNNLPSDQYSDAIKQQEQQKMPNSYSPYASYIASTAEKLKKLVENKNKLNQMLLQLTRMFQNKMIPTGLYNSRRENIVKNINLLNKEEASNKRLFRQLQAFESGYERMNSTNNANQPVLHNRNIPNYMRQYNSEKKEEDEAQMQEYTESLGINPTAFGGVYSAEERESIRSFLEEFKTRETEIEGETMTPEELTVNLMKHQRLGLHWLLKMEDSVKKGGLLADEMGLGKTLQMVSLVLCNRSEDKDCKTTVVVAPVSLLDTWQGEFETKVKDSANISTLMFHDKTKVKSFRELSKYDVVFVSYHTLRSELTKTWPRRVAANGVYVDRNGEDRVVPNNGMDLETLLSLKEPGEYYSPFFTMDSKFFRIILDEGQQIKNKSSKVSKTCCTLLAKYRWILTGTPIQNNMSDLYSLVRFLRIPPYNKEAYFQKEISNPLSLKKDNNSSSSNSRVAAMNKVQVLLAGIMLRRTKNTQIDGKPLLELPPKTVSEEQCSLVDAELEFYQDLETKNKKIAEKLLKRHAKGNYSSILTLLLRLRQACLHSELVLIGEAKAEQARVASGRDFDKDWVRLFELCQDIPQSVVSRTNNFVETDMICERCTNPMELSLASVFPSCGHMICFECLKAYKEDCSERELTRYNNAGQLVFPCHICQTEQVEDSVMSFKFYDQVVNQNYSLRELRDEFDVEQHAAKEKLKEGYEIDFENLEPSPKVQQCLDLIQKIADNDPTEKVLIFSQFTTFFDILQHFVGKNLGMQSLRYDGTMNVRAKTAVLKEFQRDPNTNILLISMGAGNAGLTLTSANHVILADPFWNPFVEEQAMGRAHRISQTKEVHVHRLLVRSSVEDRIVELQNKKQELVGAALDNKKITEISRLGSRELGFLFGLNQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDN1</accession>
    <name>A0A1E5RDN1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">mitogen-activated protein kinase kinase kinase</fullName>
            <ecNumber evidence="2">2.7.11.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g2080</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDN1"/>
    <dbReference type="FunCoup" id="A0A1E5RDN1">
        <property type="interactions" value="206"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDN1"/>
    <dbReference type="InParanoid" id="A0A1E5RDN1"/>
    <dbReference type="OrthoDB" id="266718at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029458">
        <property type="entry name" value="Ras-bd_By2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584:SF369">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14847">
        <property type="entry name" value="Ras_bdg_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01304">
        <property type="entry name" value="Ras_bdg_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM" evidence="13">
        <location>
            <begin position="6"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="378"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="426"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="215"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="450"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ84991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="703" mass="77985" checksum="95CCD7E43953C5C1" modified="2017-01-18" version="1">MTDTSAETMSLHSVLKEIKCSDYLSLFQDSGITDLEDFKFIDKTILKEIGIKAIGDRLLILNKATELKDVSTAEKIAQMTKKIKDLSVTSIQPVNEELVTDKNTVIFILNDGIAKKVNVSGCFNADSIKAKLIKRLTTKNTNPKDYDVFLVDYTKNTLNLLYDVELVTISHSSDRIEKNRFLFVPKDETPTDAAVATSQKIYLKTLSMSYHPGANSGNSGNKNGQSGHDGKLLATGENKYTDKVPKLEHKKSHQFRKVFDQRPAGELISSNLQEYFPDTDMKKLKTTVRNSVRQSYRLSVYGNARDNKKAFANNVGGVILNNTEAVDKALMEDVSDTASVYSRQSICASLTGDDDLEGFDDLSSIASLSNNVHAPKNWLKGAKIGSGSYGNVYIGMNSETGELMAVKQVEFPKVVIDEATLAASNALGQKPANSGEDMPESNDAAERGNKNNNINNNNNNNNNNNNNKLAVAKSSTNHKMIDALKHEMDLLKDLHHENIVQYYGSSEENSSLNIFLEYVPGGSVSSMLSNYGPFEESLIKNFVRQILVGVSYLHRKNIIHRDIKGANILIDMKGTVKITDFGISKKLSPISRHTLNKRASLQGSVYWMAPEVVKQVTITSKADIWSVGCVIIEMYTGKHPFPEFSQMQALFKIGTNVIPDIPSWAGKDAKSFLMKTFALDFRKRPNSIQLLSHSWIDASKLQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDY9</accession>
    <name>A0A1E5RDY9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">U1 small nuclear ribonucleoprotein C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1977</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDY9"/>
    <dbReference type="InParanoid" id="A0A1E5RDY9"/>
    <dbReference type="OrthoDB" id="3973112at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030627">
        <property type="term" value="F:pre-mRNA 5'-splice site binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000395">
        <property type="term" value="P:mRNA 5'-splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000690">
        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013085">
        <property type="entry name" value="U1-CZ_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017340">
        <property type="entry name" value="U1_snRNP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31148">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31148:SF1">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06220">
        <property type="entry name" value="zf-U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50171">
        <property type="entry name" value="ZF_MATRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Matrin-type" evidence="9">
        <location>
            <begin position="4"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="211"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="345"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="363"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="405"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="472" mass="54338" checksum="96F72020AE369763" modified="2017-01-18" version="1">MARFYCDYCRSYLTHDKRSVLKAHITGKHHIRLVKDYYRNKYVEQWKRTGILNKRKVIRDHTNRNLALAKPKNNTRLNVDKNSSTFPKRLELQDIAPNYKFEKLSKDELHLNKLNRTRKFKRIRKRQQLRNLIRNVDPFNHESILRERRLRNFGDLSNVNGIENGLSILNHLYKYSPGYNKVFISTNRFDNAEYSGLKPLTSSVAKASNIYGKGKGNKNKTFKGKANTSSKASKDSKQSSVSMKQLQMTSRNTIFHDDVNTLGFMKNSNKDRKKYTMNFLKPPKVVVNKRLNLNTDSNSVNKAITGTAPGREFLFKQNNLFYKVFLRSILPKRKFLQIFHGKRSNVGNKKNNKGLELRRSRFQNKSVAADNNKNLTPSRFSNGRPTALNLHSTASGTGPAKPPSRFNQSSTGTSRFAPSTQRTTTYASTSNNSRYNPQPPHTTTQRFARSGPTRGAFRNGAKSXNPKSYRKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE11</accession>
    <name>A0A1E5RE11_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2158</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE11">
        <property type="interactions" value="866"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE11"/>
    <dbReference type="InParanoid" id="A0A1E5RE11"/>
    <dbReference type="OrthoDB" id="242910at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071561">
        <property type="term" value="C:nucleus-vacuole junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055231">
        <property type="entry name" value="2AA_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045162">
        <property type="entry name" value="Vps15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17583">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17583:SF0">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22956">
        <property type="entry name" value="VPS15-like_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="27"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1448" mass="164345" checksum="F04B7804DDD99143" modified="2017-01-18" version="1">MGQQLSITAQTAPSIAISSYVDIIKDVHYLSQLNSSRFLKTCKALDRHGEIIIKIFLKTDPLPLKHILDQVAADAVKLQDLPNVLHYATVFETERAGYLIRQHMNSNLYNRLCSRPFFKNIELKFLCFQLLNVLNNVHSRGCLHGDIKLENLLITSGNWVLLTDFSKNIKPVFIPEDNPNEYSFYFNTSQRNACYLAPERFNNKKLSKSYDEFAGLTKEMDIFSAGCCVFEIFKEASEPLFTFSEIFKYKNNELDIRQKLSQEFQNDKYICELIMDMVDLYSEKRLNAGELLDKYRNTIFPDYFYSVMYTIVQQLVTHDSDFSSTFGKPISNQMNTKFNISLPNKFLSLQVTTESVFXSFWEKLETVCSSMHYPMKKSHADEHLPIDAPFVKLCTSTTIQLQKYDSFKGKEPSVVDQSALSIIAVLTSFLRTLKTSQSQIYCMDTLIVLSQYIDDGSKIDNVLPYFVMMLSSKNSEVQVVALQKCLLLLGLLSHIDQDNESLLIDYVLPKFRFLLQNMKHDIYGKIVFAQSMSALTNVIFSFHDLVLENHLNSDDEIDENILHFNSKVLKYMELLTVALLTDENTHVRIAFLKNIAPICRLFGKERSSDIILSHLITYLNDKSAALRVQFIETISGISVLLGTISFEQYILPLMVQALTDPEESVATTTLKTLYSMVKIGLLKAQYFADTCANVAPLVLHPNENIRQFSLSIVVCVMSKMRTSEKYCILYPIIKPYFDFELEMTQDLMTLGVKKSLPRKIYNLFYTWCLNFSNSLFWKKKTQDTNVDVFGNNLVEFVTRDYLTKTYGFRGSNLPKEMLLSKDHHTSLDVPLSMEDKKWMDKILKAGLKEDDTWKLAVLRTYVFKVAKMSPRKPFEKSFQNEPLVLEHLLTERRILPRNVFLKINFLPEYRKETLHIISKPKNITVXHPSAVMDSDFSTNEDSIDQLPPKAISSNGSLFLANKAKPTRVSNFDNVYVQLESSSSLPVAGANSIRLKEAVKTKYTVSNSYEGTSEPIKKFLKTXSVSPSITSLKEFGAFVDXTNEIKQESASKLQGTYSSCVAENDDNGTSTVTAVACSLFKPHMVIGTDTGRIEIYDIQQLHENKIFSSLFHFNVKQEITSIEFLQGFDVFVVATKEGRIMLYRXELNEGQLSLTLSNVQIVSVYDFDNEKEFGIAIQTMGGSNLNCFFVLSNKGNIIEFDAKTMKPLLVFTNERKHGAVTSFTVAPRKDWMVVGTIKGVLTLWDLRLNCVVKVWCFGDCTPIYNLFDYSFFNKQESSEDRKILVVGGSSTCTASLWXLTKLQCKAVIAKSVTDSTVKIDDFVARDVDFDTSAPSIDALQLNAFVLQVVKDDILIWDSNKKDITSLSLLNGKTSGRVLVGSEKSIGGYKVTKLTSAFSIVERVNTSVTEKHSRSDLNNNDVEFLKALHTTQGLPILAIVSNGGRIDVYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE59</accession>
    <name>A0A1E5RE59_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">General transcription and DNA repair factor IIH subunit TFB4</fullName>
            <shortName evidence="14">TFIIH subunit TFB4</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 14">RNA polymerase II transcription factor B subunit 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g2165</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 14">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the 7-subunit TFIIH core complex composed of XPB/SSL2, XPD/RAD3, SSL1, TFB1, TFB2, TFB4 and TFB5, which is active in NER. The core complex associates with the 3-subunit CTD-kinase module TFIIK composed of CCL1, KIN28 and TFB3 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the TFB4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE59">
        <property type="interactions" value="1179"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE59"/>
    <dbReference type="InParanoid" id="A0A1E5RE59"/>
    <dbReference type="OrthoDB" id="17307at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004600">
        <property type="entry name" value="TFIIH_Tfb4/GTF2H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12831:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12831">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIH TFIIH , POLYPEPTIDE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03850">
        <property type="entry name" value="Tfb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="190"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="193"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU368090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ85192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="404" mass="45608" checksum="ECC85FB6B36341AF" modified="2017-01-18" version="1">MDNITDPAFYQNKLKNFVTEETPSLLTLVIDTNPAIWGFDEDKDESELINALESLVVFLNAHLAFNTSNRVCVIAAHAEGIQYLYPKTNNDLIEAYEENATKTQLEKKVFEEENWHKPTSLEENEEEDWLKPLDQPKTAEDEKADMMGDGLFXKKNSNIINKEMYAPFRKVDEAVLEELYHLFEMERARSMAEKQQQQQESQDGEQNTTNRKVTKSSISGAMSAALTYIHRTLKDNLNLRARMLVVTSGSKNEEKSEIFQYIPIMNCIFTASKLKCPIDVVKIGGTPQATFLQQATDATNGVYLHVENTKGLIQYLSTAMFIEPALRQIIVKPTKGTVDFRTSCFLTGKVVAIGYVCSVCLCVLSLIPPGNKCPACGCEFDEKIIKKLKRKPIVKKVSNGVRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE68</accession>
    <name>A0A1E5RE68_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sorting nexin MVP1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Sorting nexin mvp1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1830</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sorting nexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE68">
        <property type="interactions" value="168"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE68"/>
    <dbReference type="InParanoid" id="A0A1E5RE68"/>
    <dbReference type="OrthoDB" id="10064318at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07597">
        <property type="entry name" value="BAR_SNX8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06866">
        <property type="entry name" value="PX_SNX8_Mvp1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000042">
        <property type="entry name" value="Sorting nexin mvp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028662">
        <property type="entry name" value="SNX8/Mvp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035704">
        <property type="entry name" value="SNX8/Mvp1_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045734">
        <property type="entry name" value="Snx8_BAR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47554">
        <property type="entry name" value="SORTING NEXIN MVP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47554:SF1">
        <property type="entry name" value="SORTING NEXIN MVP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19566">
        <property type="entry name" value="Snx8_BAR_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="12">
        <location>
            <begin position="194"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="134"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="487"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="134"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="159"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ85208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="593" mass="68229" checksum="3FEAF54275945B5D" modified="2017-01-18" version="1">MDSFLEADPWASEADNKNPATSSPFHNTNTSIFTSKKDDGTNSYHGATSSQLNAWNTHNLDELVASNSSITNHTSATMSPSNSDENLQSRFGNLSVGNMKAQASVWGNELSNSNLGSLFGGARSVLENNLPGSSSLSYSKQHETPNTVRYDTGAINEREDGEGEDNGIEDDEETNEDDFKAWLASIRRLYNPLYYDLISIEELPEREGLLFKHTNYLVKYSSGQPATKKSVEGARHTDGAVGESGSSRGKVIRRYSDFVWLQSVLLKKYPFRLIPELPQKMIGSQNTDPVFLERRRLGLARFANLVMKHPVLKNDDLVITFLTVPTDLSSWRKNAPYDTTEEFLDKKIDKSFIXMWKPRFSRYWNELDNSLDLSLEYWTKMTVLIERYERRQRAAAQERYFLKQTLSKFSKESTKKLYPLDSHGLVNDINEHLGYVGKHLENCVALTLKENEEVRDTLSEKFKTFIEILFSMKGLFERYKMLAGNNVLQLQRRVELNEEKLKIMREKADIKGATYDKLEDNITEDKKTIAEQINRSWLIKECILEEFAIFHETMFLITNLFQKWAVMYSKYCDLESNEWDKLLGRLESMPTHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE96</accession>
    <name>A0A1E5RE96_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">GTP-binding protein ypt1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2350</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endomembrane system</location>
            <topology evidence="6">Lipid-anchor</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RE96"/>
    <dbReference type="FunCoup" id="A0A1E5RE96">
        <property type="interactions" value="1144"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE96"/>
    <dbReference type="InParanoid" id="A0A1E5RE96"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01869">
        <property type="entry name" value="Rab1_Ypt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057289">
        <property type="entry name" value="Rab1/Ypt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="177"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ85238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="214" mass="23580" checksum="024D049682CC48DF" modified="2017-01-18" version="1">MSGDYDYLFKLLLIGDSGVGKSCLLLRFADDTYTQDYISTIGVDFKIKTIELENNKIVKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTSLESFQNVKTWLQEIDRYGNTGVLKLLVGNKNDLVSQKQVDHETALNFANSLNLTLIETSALNSENVEEAFVTMAKQIKENMDKNAANNGNNGAGDQQNNGGSNVNLNNGKSLTNAGASNCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REI3</accession>
    <name>A0A1E5REI3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1849</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5REI3"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="358" mass="41576" checksum="BB04FE072DE180A8" modified="2017-01-18" version="1">MVFPALGFCFQMKSNMINIPNVGTATMLSTGIPDKKTRESSYFIDLIKLREKNFISDTKVGLSALXINYNEXKSAKYEQTSLSLFTQCKKEAEEEAEDXEDXGSEDEEENFEKSVVFDTTDXEINXXHDNKEKTYGEHFRERKRDCLKRITLSQVDKIFKVGDDQIQNLNNNKPIRKRDCVKNFVSTKIRKEYDGNXXYKTXTHNKSATSVNQWETHSASRQFQKNNXQNEIVEPQNKSLIXTSLFRKGLAATTDRTSNHELMSKXLSSFSIKCTHQKXSVVKCYQDTNAKRRRRKAKFIKYAQKRAADAMVKEITPIMFNLVFNVLWWGAITKIKTVFRRCIEFIQTNVFGFFFKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REW9</accession>
    <name>A0A1E5REW9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 8">Mediator complex subunit 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MED4</name>
        <name evidence="10" type="ORF">AWRI3579_g2001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REW9">
        <property type="interactions" value="279"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REW9"/>
    <dbReference type="InParanoid" id="A0A1E5REW9"/>
    <dbReference type="OrthoDB" id="1929813at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019258">
        <property type="entry name" value="Mediator_Med4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13208">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13208:SF2">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10018">
        <property type="entry name" value="Med4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="200"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="200"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="291"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="308" mass="35067" checksum="4E29958D264ED36F" modified="2017-01-18" version="1">MSSLKQSASSASMSNAVLEGSQSSNVTLAKGNSATENTILHLPLYTQLDXYEXALSKLVSGVDSFKPDLQDAHDLIKADKMLVESLSKFPEYNDINDQLLELRAKKQSIDDKNKKILLTLNDCYTKLNTLPMVEQVQFENEILIKQREFLKAKDVLEYAMKLSKFTKIPPTFDKGMIGPINFVWPGDDSLRKGMLAMADVQEREKQSHKHELSLEQQAQIQQQQEQQQEALSKEQPGVPESKEETLIFDGKHKEEPKKQEQGNAQESQEGHNNKEDENEEEEQKATSDKEQQDEDLDLELDLFNPDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REX6</accession>
    <name>A0A1E5REX6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleotide exchange factor SIL1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2256</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Interacts with KAR2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIL1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REX6">
        <property type="interactions" value="173"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REX6"/>
    <dbReference type="InParanoid" id="A0A1E5REX6"/>
    <dbReference type="OrthoDB" id="448649at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031884">
        <property type="entry name" value="Sil1_fungi"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16782">
        <property type="entry name" value="SIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184716" description="Nucleotide exchange factor SIL1" evidence="10">
        <location>
            <begin position="24"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="429" mass="49548" checksum="A6C13F994F988008" modified="2017-01-18" version="1">MRVTLQHCLSLLISFAWLQKTFCNPITLVPAIVESSTEESXPTTLEKQDQLEDDLTINVDLNLVCNTQLCYPLEFEPLSEWQEIKPKQIIPKGLDIRINMDTGKKEAKLAAPAPAAPAPASDPDVSSSLADKQNFASHKEQGLEQLNNEEDLHEFVSYFEXMQQDIDENNLQKIIGNLDXLMEFSHDYKHGYKLIKYEQPILMELIFHDNSNLQIVEMTTRLIAACLRNNPPAIEAFYPTNVDKILDALMVFKNQTIIKRFINILQNVAPKASNLDQVYGTLMHVYFSTTDQDIKIKILDILSTLELNTAHTRGKSIMKRSTDSDAVKVWFNEYCKQIQQEDLDEFHIRKFYKTLAQLKKETGFKIEDKNFMKWISXQAVQRKKHLDSSVNIEQRDLEQDDFNKEFIEARHAVFGNPMAKRIKDFQDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REY2</accession>
    <name>A0A1E5REY2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">DASH complex subunit DAD3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Outer kinetochore protein DAD3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g2029</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex DAD3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REY2"/>
    <dbReference type="FunCoup" id="A0A1E5REY2">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REY2"/>
    <dbReference type="InParanoid" id="A0A1E5REY2"/>
    <dbReference type="OrthoDB" id="2443965at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013965">
        <property type="entry name" value="DASH_Dad3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28017">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28017:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08656">
        <property type="entry name" value="DASH_Dad3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="15" id="KW-0137">Centromere</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ85123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="90" mass="10208" checksum="2861C36AF52A180C" modified="2017-01-18" version="1">MDQDDSLTALQETVLGKYRLLADELHTLDDTIKKIINNEHKDEDGNTVRCSPQELLQLVRELEVKMGIAGTLIKGSVYSLILQRAQQEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REZ3</accession>
    <name>A0A1E5REZ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Phosphomannomutase</fullName>
            <ecNumber evidence="5 13">5.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g1963</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>alpha-D-mannose 1-phosphate = D-mannose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11140"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="EC" id="5.4.2.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the eukaryotic PMM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.8" evidence="5 13"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REZ3"/>
    <dbReference type="FunCoup" id="A0A1E5REZ3">
        <property type="interactions" value="730"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REZ3"/>
    <dbReference type="InParanoid" id="A0A1E5REZ3"/>
    <dbReference type="OrthoDB" id="10264771at2759"/>
    <dbReference type="UniPathway" id="UPA00126">
        <property type="reaction ID" value="UER00424"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004615">
        <property type="term" value="F:phosphomannomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02585">
        <property type="entry name" value="HAD_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1240.20:FF:000001">
        <property type="entry name" value="Phosphomannomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005002">
        <property type="entry name" value="PMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043169">
        <property type="entry name" value="PMM_cap"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10466">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10466:SF0">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03332">
        <property type="entry name" value="PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00445">
        <property type="entry name" value="alpha-phosphomannomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01143">
        <property type="entry name" value="C2.B.3:_Phosphomannomutase_Lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01140">
        <property type="entry name" value="C2.B:_Phosphomannomutase_and_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="10">
        <location>
            <position position="21"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ85472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="254" mass="29159" checksum="684327A3295CF8D5" modified="2017-01-18" version="1">MPIQEFAYKENPETLVLFDVDGTLTPARLTISDEVRDTLKKLRQKVCIGFVGGSDLSKQVEQLGPTVLNDFDYCFSENGLTAYRLGQEMASQSFIGWIGEEKYNKLVKFALRYLSEIDLPVRRGTFIEFRNGMINISPVGRNASTQERNDYEKYDKEHKIREKFVAALEKEFPDYGLTYSIGGQISFDVFPTGWDKTYCLQHVKNDGFKEIHFFGDKTFKGGNDYEIYEDPRTIGHAVNSPADTVRILKEIFHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF16</accession>
    <name>A0A1E5RF16_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">L23</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">RP15L</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">RP33</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">YL14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2133</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. eL19 may play a role in the last stages of translation initiation, in particular subunit joining and shedding/releasing factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes). eL19 lies in close proximity to the binding site for eukaryotic initiation factor eIF4G.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF16"/>
    <dbReference type="FunCoup" id="A0A1E5RF16">
        <property type="interactions" value="1021"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF16"/>
    <dbReference type="InParanoid" id="A0A1E5RF16"/>
    <dbReference type="OrthoDB" id="5407653at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01417">
        <property type="entry name" value="Ribosomal_L19e_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.240:FF:000001">
        <property type="entry name" value="Ribosomal protein L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1650.10:FF:000001">
        <property type="entry name" value="Ribosomal protein L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035970">
        <property type="entry name" value="60S_ribosomal_eL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039547">
        <property type="entry name" value="Ribosomal_eL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000196">
        <property type="entry name" value="Ribosomal_eL19_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015972">
        <property type="entry name" value="Ribosomal_eL19_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033935">
        <property type="entry name" value="Ribosomal_eL19_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057259">
        <property type="entry name" value="Ribosomal_L19e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057260">
        <property type="entry name" value="Ribosomal_L19e_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006343">
        <property type="entry name" value="PRK08570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01280">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25476">
        <property type="entry name" value="Ribosomal_L19e_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01416">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48140">
        <property type="entry name" value="Ribosomal protein L19 (L19e)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 12" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL19" evidence="11">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="52"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="145"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="67"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00065512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00075826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00080122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00082767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00083500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ85491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="184" mass="20992" checksum="86E85A475AE562B1" modified="2017-01-18" version="1">MVPVLAAAVAKVGKRKIWMDPNEASEIAQANSRSAIRKLISNGTIVKKPTRIHSRSRTRMMAESKRNGRHGGYGKRKGTKEARLPSQVVWIRRLRVLRRLLAKYRDAGKIDKHLYHSLYKAAKGNAFKHKRALVEHIIQAKADAARENALKEESEARRAKNRSLRERRAQRAVEKKEAMLQESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF34</accession>
    <name>A0A1E5RF34_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Delta(7)-sterol 5(6)-desaturase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2399</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF34"/>
    <dbReference type="FunCoup" id="A0A1E5RF34">
        <property type="interactions" value="192"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF34"/>
    <dbReference type="InParanoid" id="A0A1E5RF34"/>
    <dbReference type="OrthoDB" id="6354873at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="259"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="6">
        <location>
            <begin position="191"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="371" mass="43868" checksum="21559317AF0CC1F2" modified="2017-01-18" version="1">MDLVLEFCDSFGFDKLYSKVFPASMAENFSPEWQSRLGLNCGNIFNSTLLQMTGNIYKTDRSQWTVNPEIYGHVPYLFNMSPQTFETMLPRYNILRQILSLFVVTTIFGWALYLICATFSYVFIFDKAVFNHPRYLKNQMSLEIKLAMSAIPGMVLLTIPWFIMELHGYSCLYMEIDYKNHGIKNFIMEYVYFILFTDCGIYLAHRWLHWPKVYKALHKPHHKWLVTTPYASHAFHPVDGYFQSLAYHVYPMLFPLHKVSYLILFTFVNFWTVMIHDGEYLANDPVVNGAACHTVHHLYFNYNYGQFTTLWDRLGGSYREPDQELFDKSLKKSTKTWDEQIMKMEEIKKVVEGDEDDRVYGTEEKYLKKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF64</accession>
    <name>A0A1E5RF64_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1754</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF64"/>
    <dbReference type="InParanoid" id="A0A1E5RF64"/>
    <dbReference type="OrthoDB" id="3997736at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="221" mass="25960" checksum="B5B08B3FD2D17150" modified="2017-01-18" version="1">MFRSRIFRAVSKRNVITPPTYFSSPAHSPNVPLHHVPPMRSGSQAEPQPRPRLLDISILFSVLSLSFFAVDNYRSRVELELELENFRLDSLNQKDFSKMELQGKFKKRELQILNERKNTQIKEMKMLLHIAMLKQQLEKNNIKDSITLQQVHEEFNKKIRMDNSLSNVSGVKLWIDSDVDSGKISADWTKRSKEDNARYNVDDSIKKYLPNEKEYEHLRDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF93</accession>
    <name>A0A1E5RF93_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dolichyl-phosphate beta-glucosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.117</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g1862</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a di-trans,poly-cis-dolichyl phosphate + UDP-alpha-D-glucose = a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate + UDP</text>
            <dbReference type="Rhea" id="RHEA:15401"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19502"/>
            <dbReference type="ChEBI" id="CHEBI:57525"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.117"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:15402"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.117" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF93"/>
    <dbReference type="FunCoup" id="A0A1E5RF93">
        <property type="interactions" value="651"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF93"/>
    <dbReference type="InParanoid" id="A0A1E5RF93"/>
    <dbReference type="OrthoDB" id="3784at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004581">
        <property type="term" value="F:dolichyl-phosphate beta-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04188">
        <property type="entry name" value="DPG_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035518">
        <property type="entry name" value="DPG_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859:SF91">
        <property type="entry name" value="DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859">
        <property type="entry name" value="GLYCOSYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="14">
        <location>
            <begin position="71"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ85580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="335" mass="37753" checksum="CEEAA9A0622EBEDC" modified="2017-01-18" version="1">MMNTTAIATVAVTTVVALLASIYTLVYLFSNQPRSVEPSEQVSSYYDYDSKTIKKAQLPQTPLHKSELKFSIVVPSYNETERIYVMLREALEYFAQNEDYSKEISTAKDAYEILIVDDGSRDGTFQYVLDLCRQDEFFAKVLSTTNGVIRCIQLSKNRGKGGAVRHGMLHARGEYILFADADGASKFADSGKLITSIQDKTESGKAAIAIGSRAHMVNTDAVVKRSLIRNILMYGLHTLVFIFGIRSIKDTQCGFKLFNRKSMLSIFPFLHTERWIFDVEILILGIRKNIDIHEVAISWHEVDGSKVDLARDSINMAIDLVVIRLAYLFGIYKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFI8</accession>
    <name>A0A1E5RFI8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 18">Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial</fullName>
            <ecNumber evidence="5 18">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">AWRI3579_g2320</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a quinone + succinate = fumarate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="18">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
        <text evidence="18">Binds 1 [3Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="18">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 18">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Mitochondrion inner membrane</location>
            <topology evidence="1 18">Peripheral membrane protein</topology>
            <orientation evidence="1 18">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="5 18"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFI8"/>
    <dbReference type="FunCoup" id="A0A1E5RFI8">
        <property type="interactions" value="700"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFI8"/>
    <dbReference type="InParanoid" id="A0A1E5RFI8"/>
    <dbReference type="OrthoDB" id="1696654at2759"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER01006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000007">
        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1060.10:FF:000001">
        <property type="entry name" value="Succinate dehydrogenase iron-sulfur subunit SdhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050573">
        <property type="entry name" value="SDH/FRD_Iron-Sulfur"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004489">
        <property type="entry name" value="Succ_DH/fum_Rdtase_Fe-S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004616">
        <property type="entry name" value="PRK05950.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921:SF29">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] IRON-SULFUR SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE IRON-SULFUR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13534">
        <property type="entry name" value="Fer4_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="16 18" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="8 18" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="14 18" id="KW-0408">Iron</keyword>
    <keyword evidence="15 18" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <keyword evidence="21" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="19">
        <location>
            <begin position="37"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="20">
        <location>
            <begin position="159"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OEJ85672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="256" mass="29158" checksum="4BA9E1336E402A09" modified="2017-01-18" version="1">MLRQAIMKRGLATAAAPATHVPKMKTFKIYRWSPDTPEVKPKMQEFQVDLNDCGPMVLDALIKIKDEQDGSLTFRRSCREGICGSCAMNIGGTNTLACLCKIDKNESKTTKIYPLPHMHIVKDLVPDLTQFYKQYKSIQPYLQRKTFPKGKEIYQSVEDRKKLDGLYECILCACCSTSCPSYWWNAEEYLGPAVLMQAYRWMVDSRDESFGKRKEMLQNSMSVYRCHTILNCTRTCPKGLNPGKAIAEIKKALAVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFK0</accession>
    <name>A0A1E5RFK0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Protein SEY1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SEY1</name>
        <name evidence="12" type="ORF">AWRI3579_g1837</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="8">Enriched in the cortical ER. Concentrated in punctae along the ER tubules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family. RHD3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFK0">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFK0"/>
    <dbReference type="InParanoid" id="A0A1E5RFK0"/>
    <dbReference type="OrthoDB" id="1597724at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016320">
        <property type="term" value="P:endoplasmic reticulum membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01851">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000727">
        <property type="entry name" value="Protein SEY1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03109">
        <property type="entry name" value="Sey1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008803">
        <property type="entry name" value="RHD3/Sey1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046758">
        <property type="entry name" value="Sey1/RHD3-like_3HB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45923">
        <property type="entry name" value="PROTEIN SEY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45923:SF2">
        <property type="entry name" value="PROTEIN SEY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05879">
        <property type="entry name" value="RHD3_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20428">
        <property type="entry name" value="Sey1_3HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="8">
        <location>
            <begin position="1"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="8">
        <location>
            <begin position="846"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="848"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="8">
        <location>
            <begin position="870"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="domain" description="GB1/RHD3-type G" evidence="11">
        <location>
            <begin position="167"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="52"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="895"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="906"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="177"/>
            <end position="184"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ85654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="922" mass="105515" checksum="4B84AEA93B52D35D" modified="2017-01-18" version="1">MDPDHSSTLSKNSRTAGLNSPPSSPSKSSKSLQSVDTGASPITVAEVATMLEHPVKGEIGPTTETTTTTTETAPEGAENGVEEETVTVTTIEAPNEVKTITKTETPYEIKTETKTETPTETIVETAVETKLDKNDLPIQLISEEKQYNSSTLEFFKKTIXNKADIDKVNYHVISVFGSQSSGKSTLLNNLFHTNFDTMDAQVKRQQTTKGIWLGHAKEVATTASEHMKPDRDLFVLDVEGSDGAERGEDQEFERKAALFALAVSEVLIINMWEHQVGLYQGNNMELLKTVFEVNLSLFGKSKMEGHKVLLLFVIRDHIGVTPIESLKASLLNELEKMWEGLSKPQGTESTTIFDFFDFEFKGIGHKYLQYDQFVADVKTLGDSFTTKNPAESYFKPEYHHNLPLDGWNLYSESCWMTIENSVELDLPTQQILVAKFKTKEFSDAAFEKFKNEFEGYNHTDLTHEDIAKHLSSLKMSCFLAYDEFASRYNKQVYQDERQVLSENVHHILVDFVMQKAEELEHELLVAFKELLNSSKKTKSFVNRSQSAKSNVAEKFEAMLSVFFKYELIDETDSADIAKKFNEVLIKETETERGKEFDQLVRRFERSMKLKLQESLELMLMEPKVTFWKDYNDKFHQLFNELLGKKFKSADXDTVDFKLGFSTEENEAKFVAFKKQSWKIWKEVIDSQLTISKLPSILEETFKINFLYENNDSPRFFTNVAQIDEFXKEAKDFSLQMLDIYSVARLSDESSEKIKPDVSLFSDDIEREESEQQEEEETENDFAIKRDFGDILTDNEKEKVRIEFNKKVQLVILQTKRSILTSNTKIPPYMFLLLGVLGFNEFMMILRHPLLLTLCILTLTGWYFVHRTGSYRAVQHVAASSLQGMKDATVEKLRDALTTEQSSSTKSQRESFEMQEMSSKKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFK4</accession>
    <name>A0A1E5RFK4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">chitinase</fullName>
            <ecNumber evidence="3">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g1988</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFK4">
        <property type="interactions" value="165"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFK4"/>
    <dbReference type="InParanoid" id="A0A1E5RFK4"/>
    <dbReference type="OrthoDB" id="6020543at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02877">
        <property type="entry name" value="GH18_hevamine_XipI_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000125">
        <property type="entry name" value="CTS1p Endochitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045321">
        <property type="entry name" value="Cts1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050542">
        <property type="entry name" value="Glycosyl_Hydrlase18_Chitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708:SF49">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="5" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184724" description="chitinase" evidence="15">
        <location>
            <begin position="24"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="16">
        <location>
            <begin position="33"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="325"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="489"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="17">
        <location>
            <position position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ85688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="605" mass="63706" checksum="EB584935993F408E" modified="2017-01-18" version="1">MYSLITKICALQFLSAMFLSAAASTTFDDNANNHVALYWGQNSAGSQESLATYCQSGEADIFLLSFLYEFPDPLNVNFANACSDSYXDGTLKCTQIAEDIKTCQSLGKKILLSMGGASGAYGFSDNATAVAFASTLWNTFGEGSADERPFGDSVVDGFDFDIENNNDVGYTALVTELRSIFADQGSKQYYISAAPQCVYPDAGVGNLLLNADVDFAFIQFYNNYCNVGTNFNWDTWTDFAANSAFNKDIKMFLGLPGSASGAGSGYISDLSLVDSTVADMAKDSHFGGVMLWDASQGYANIVDGENYVVNMKNIVDKYATSTTSATSTTSASSTTSTTSTTSTTSTTSATSTASSSSSATKSAAKSTSTTTSSSSTKTSSPSTTKSQTTTSILFSSTTFTTSTRNWDEPLTTETVTLANGQVTTSHIWWLPETTTYAAATTTTGRNWNEPLTTETITLANGQVTTSHIWWLPETTVSVTSETTSASFAKSTTTLAPTQTPTTSSSLATTTTASSTVTAASTASATSSAQASAIALNEQYAAGKMSGKDTCSTGDMACDAEGRFALCANGIWSYFDCSAGTTCFAYTSNEEVLLGCNWSSVKSQFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFM5</accession>
    <name>A0A1E5RFM5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2191</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RFM5"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="320" mass="35038" checksum="C34880445A74D3D1" modified="2017-01-18" version="1">MSTNFDSALAMKRSLSSTSYNGICNPAPVPSSSPSFSSGDAYGNNNXNSNVNSNGNKNNHISYNNFGIAQAQPGIAAFGAQNPHISQNFVPPQSTLSSSNSNTFKRRKRARDQISDNSYGNLATVSHDNAQQSNRTMFPTPQGMQTSPFHVSPTDIQDPLHASPSSNFDMEEQMDVSPSPPSSNQPSNTPSVFSYGPSHNQYXDYNSVEFVEDYMAEGYDLKQIPVMHNSLDTDTDXAQLQSNNNKKMCVTYPYSHATNVTQTDVDPANTXYHNSNMNEEMQFDPLIPSASDYRTVNSSYSASLDDITGAQYSLYDDVDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFU0</accession>
    <name>A0A1E5RFU0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Structural maintenance of chromosomes protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g2419</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFU0">
        <property type="interactions" value="763"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFU0"/>
    <dbReference type="InParanoid" id="A0A1E5RFU0"/>
    <dbReference type="OrthoDB" id="5575062at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03275">
        <property type="entry name" value="ABC_SMC1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028468">
        <property type="entry name" value="Smc1_ABC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937:SF12">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="13">
        <location>
            <begin position="542"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="309"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="437"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="703"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="825"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1044"/>
            <end position="1081"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ85453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1244" mass="142581" checksum="9BA3A4D8350F752E" modified="2017-01-18" version="1">MGRLLGLELRNFKSYKGTVKVEFGDSDFISIIGPNGSGKSNLMDAMSFVLGVRSSQLRSSVLKDLIYRGKLPNPEPVLEPVVESNKENESDDLSISKQXPSNSASVSAFYLKTSSADNETETVEFERSVTSKGESFYKINGKIVSYKYYTETLEAENILVKAKNFLVFQGDVEQVASQNALELTKLFEQVSGSIQYKKRYEELNEQYKHALQMSSDGLFRKRRLQSELIHYKDGVSKDEKYKAKVAQRKHLWIQYTLWQLFQLEQKKTALIEMYDANKEQIKATKKDIAKEETSISAAKAHSSKEELKILKMGDDINMLELKLESLKSQLLPLIQNKRSSNRKMQQLETKIESFERDMERQESILQNLERNHKVVSKSKKQYEDELLKSKNSKYHLDDESLEEYNRLKEVFLTSGGLELEDKLALETSSGNELKNELKKFDDQINNSQEFIDNLTKESEEAHMEFKKLTEALNDKNSQHSELSSKLKNLQSNMQKLNREETQTNYKLRDALMKIDELSAEQRESKKDQRLRENVNSLRRLFPGVKGLVNELCKPKKDKYSTAIAIILGRNFNSVIVDSISTAQQCISYLKEQRAGSATFIPLDTVEVSPPRRVQLEQCILAIEALNYDPTLEKALQYVCGDAIICDDLEVAKDLKWNRNINSKLVTLNGSLIQKSGLMTGGVSKENSQNRWDKEEFKNLVSLKDKLVQTVTEIQQQIRLAQNASREIELQLDDLNNELSSIRTQLTHNERARQELSTQTEYYQNLISNEYEPRKLILEESIAVHEKALTFLNEQSTSLKQKLYAKLSKKLGFSLVEYEKTTGETLRKQSKEMGILQKELINIESKISFERDRLATTSKRIEKLKKDFDAQKDYINQLEEKEIKLTTKIEKAEDHLKDKRNELKTFKKSVDTQFKQVTGVEENLQDFTIKLNNQKKRSAVIKADSEKLDLERLNIFKNCQMTVIDLPLISGSLEDLPMDKIDSQVLESASQIEVDYDYLDEEYTAKTNSNGSLEETIGKTFENKINSIEEDLITLQPNTHAHDRLNEVQKKFDAISKEVEGLKKHEKKLSEQFNEVKTKRKELFGKIFQHAAEHIDPVYKELTKDPNSQVALAGGSASLTLEDVDEPYLAGTKYHATPPLKRFKEMEYLSGGEKTVAALALLFTINSYQPSPFFVLDEVDAALDVTNVQRVAAYIKRNATPKFQFIVISLKNNMFEKSDALVGVFRDQSLSSSQVLTLDLRPYAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGG9</accession>
    <name>A0A1E5RGG9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Chromosome segregation in meiosis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1406</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Plays an important role in the control of DNA replication and the maintenance of replication fork stability.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the fork protection complex (FPC) consisting of TOF1 and CSM3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the CSM3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ85985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGG9"/>
    <dbReference type="InParanoid" id="A0A1E5RGG9"/>
    <dbReference type="OrthoDB" id="437078at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031298">
        <property type="term" value="C:replication fork protection complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043111">
        <property type="term" value="P:replication fork arrest"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012923">
        <property type="entry name" value="Csm3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040038">
        <property type="entry name" value="TIPIN/Csm3/Swi3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13220">
        <property type="entry name" value="TIMELESS INTERACTING-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13220:SF11">
        <property type="entry name" value="TIMELESS-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07962">
        <property type="entry name" value="Swi3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0236">DNA replication inhibitor</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromosome segregation in meiosis protein 3" evidence="10">
        <location>
            <begin position="102"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="390"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="201"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="393"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="408"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="441"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="11">
        <location>
            <position position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="458" mass="51846" checksum="4F634130E17ABFA1" modified="2017-01-18" version="1">MTDADQYYMHEFDLDEDLKKPKKSRKXIATLHQTDTRGNESNADYGVNDNDPNTSFDPTINMAYLDPTENVADPSASSMAAAAAAAAAAASGRPSFRKRVNLDTDKLLDPEKGLDYVMKNARKRIRISKQNSTLDNLSSITQFYQLWAHNLYPKAKFKDFLLMAENLGNQIEIKSYRRDLYTKELEEGIDTKKYDKNVHDTNPTESNENQNNNXFIPQSKGLFVEDKTSAIPADSLGVVDYRKHESEXSFDLVEQXAAFAGXFSNKQKLEKDCHNLVEKSVTDHIGSEKFFGVNISKKYYDLFEQLGKHELLTKEMKIEIKNVMLGAVPSSYLNMLNILIENGNTWCNLEGNDSTVSEFVDLPVHQTPKGIDHSKTGKNKENEVSAQITYNDNNDDENDDDXDELISAYSQKLPNNESATPQANTQPQDNSAENRXPVEEQYGEEEEDEMALMREFDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGH7</accession>
    <name>A0A1E5RGH7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cell wall protein CWP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1373</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ85995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGH7">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGH7"/>
    <dbReference type="InParanoid" id="A0A1E5RGH7"/>
    <dbReference type="OrthoDB" id="5415592at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005199">
        <property type="term" value="F:structural constituent of cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000420">
        <property type="entry name" value="Yeast_PIR_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00399">
        <property type="entry name" value="PIR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00929">
        <property type="entry name" value="PIR_REPEAT_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50256">
        <property type="entry name" value="PIR_REPEAT_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184741" evidence="4">
        <location>
            <begin position="21"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="148"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="148"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="317" mass="31634" checksum="89B1321E24E2FA7B" modified="2017-01-18" version="1">MKYTTVALTSLIALTRFALADSQSFGLVSIRSGSSLQYSSVFPSNGVLVVGSNSGSSTSAVVTDEGYLQYDDGTYVGLSSEGQYTATTDKSQAVTGWSIEDGELKFDGEQNFIAVSTSDTYELYNHNTGSDDLGIAIVPFTKTGGRASDFAPSDSSSSSSSSSSSSATEXTATPSSTATTLAPETSATSASPVAKSTVAAVSQITDGQIQATTATSASPVAKSTVAAVSQITDGQIQATTATSASPVAKSTVAAVSQITDGQIQATTGTSASQASKSTVAAVSQITDGQIQATQSANNGAKLGSSVGLVALAGALLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGI3</accession>
    <name>A0A1E5RGI3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cell division control protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1524</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDC6/cdc18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ85984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGI3"/>
    <dbReference type="FunCoup" id="A0A1E5RGI3">
        <property type="interactions" value="1108"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGI3"/>
    <dbReference type="InParanoid" id="A0A1E5RGI3"/>
    <dbReference type="OrthoDB" id="3972687at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049945">
        <property type="entry name" value="AAA_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015163">
        <property type="entry name" value="Cdc6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054425">
        <property type="entry name" value="Cdc6_ORC1-like_ATPase_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF26">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22606">
        <property type="entry name" value="Cdc6-ORC-like_ATPase_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09079">
        <property type="entry name" value="WHD_Cdc6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="317"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="134"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="250"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="26"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="169"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="219"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="261"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="727" mass="81822" checksum="B69B2A58E9286CBA" modified="2017-01-18" version="1">MFALEPQSLKIYYRVCVIAINYNKNEEEEEEEEEEEEEEEEENTYHLSTTLFSSDFFMTLDNQTDVLSGRRRSSRLSMKLLLQQDEESGKGLEKKTEQRKLENNFDNFVSTPKKKKNLLLQGCDLKFPGFETPGPDFSDSYGTPASPTRTPVSQNSKPQKTLNKYEGLSISTKGILTPNVSPSKRKSSSGYNTHSPSRTNDDMESPKKKLFFGRPETPLSPTCTSDKPDLSTNSANVFTNIYTVIKRSLKDSGDDDDGDDNTNNNNNNHNNNNNNNNNNNNNNNAGSETPTRAFLQTRENEYTSIMSFLSRTLSKKSSSSLYITGPPGTGKTAQVNKIVNNHIKKIDTNVKSKGECSSTTRHFTDMDLKQHACQICILNCMSLKTPKNIYSHLFQTFTNDNSRGEKSLEQLQELLESKKDTSFIVVLDEIDHLCNGSVLSKPILSDLFLATKSDRYQLVLIGISNSLDITDKFLSRLKLDVNLQPESIIFKPYTQDQIFQILSAKLKTIPNAVQVVQPLALRFLSKKMSSATGDLRKVLDVLTRSIEMLELEEMRANLKKGPSDPFAADKDVSKPAVLRKVTVVHISRACNYEIDSSNTKSRIEKLNLQQKVILVCLVHKHEHDSLGNFTVDECYDYYCNRLTRDVPDLLTPVRRSEYGEISNTLEMMGLCTIIIGKGINRKGSSPNKKGTGSPSKIKYSSKTLTSQIRLEQLKKDLGELGILGKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGI8</accession>
    <name>A0A1E5RGI8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 14 17">Homoserine dehydrogenase</fullName>
            <shortName evidence="14">HDH</shortName>
            <ecNumber evidence="5 14 17">1.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">AWRI3579_g1156</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the conversion of L-aspartate-beta-semialdehyde (L-Asa) to L-homoserine (L-Hse), the third step in the biosynthesis of amino acids that derive from aspartate (the aspartate family of amino acids), including methioinine and threonine, the latter of which is a precursor to isoleucine; production of homoserine leads to a branch-point in the pathway as it can either be O-phosphorylated for processing to threonine, or O-acylated for processing to methionine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-homoserine + NADP(+) = L-aspartate 4-semialdehyde + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15761"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.1.1.3"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:15763"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:25213"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 17">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 14 18">Belongs to the homoserine dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.3" evidence="5 14 17"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGI8">
        <property type="interactions" value="363"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGI8"/>
    <dbReference type="InParanoid" id="A0A1E5RGI8"/>
    <dbReference type="OrthoDB" id="67851at2759"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00063"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00465"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004412">
        <property type="term" value="F:homoserine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009090">
        <property type="term" value="P:homoserine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000006">
        <property type="entry name" value="Bifunctional aspartokinase/homoserine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011147">
        <property type="entry name" value="Bifunc_Aspkin/hSer_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001342">
        <property type="entry name" value="HDH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019811">
        <property type="entry name" value="HDH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022697">
        <property type="entry name" value="HDH_short"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43070:SF5">
        <property type="entry name" value="HOMOSERINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00742">
        <property type="entry name" value="Homoserine_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036497">
        <property type="entry name" value="HDH_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01042">
        <property type="entry name" value="HOMOSER_DHGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14 17" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11 14 17" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="9 14 16" id="KW-0521">NADP</keyword>
    <keyword evidence="10 14 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14 17" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Aspartate/homoserine dehydrogenase NAD-binding" evidence="21">
        <location>
            <begin position="11"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Homoserine dehydrogenase catalytic" evidence="20">
        <location>
            <begin position="152"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="258"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="11"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>L-homoserine</name>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR036497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF03447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="OEJ86028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="360" mass="38850" checksum="81B2B9D4A86360FB" modified="2017-01-18" version="1">MSKAVNVAIVGSGVVGSAFIDQLLSVKSNITYNVVAILNYEASLVSKDLKPLPITTANWREYFAQHTEKKTVSPADLLSLLKQSALPAIVVDNTSNEGWAKSYPDFVANGVSIATPNKKAFSSGLALWEKLFPNDPKAGLVYHEATVGAGLPIVNFLKEIIETGDEVEKIEGIFSGTLSYIFNEYSTVEKKDVKFSDIVKVAKELGYTEPDPRDDLNGLDVARKVTILARISGLKVESPSSFPVQSLIPAPLESVSTSQEFLEKLPQYDXEIQKLKDEAAAENKVLRFIGQFDVQSQKASVGIAKYDYSHPFAGLKGSDNVISIKTKRYTNPVVVQGAGAGAAVTAAGVLSDVIKXAQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGJ2</accession>
    <name>A0A1E5RGJ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vacuolar membrane-associated protein IML1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Vacuolar membrane-associated protein iml1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1155</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IML1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGJ2">
        <property type="interactions" value="711"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGJ2"/>
    <dbReference type="InParanoid" id="A0A1E5RGJ2"/>
    <dbReference type="OrthoDB" id="39497at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990130">
        <property type="term" value="C:GATOR1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04449">
        <property type="entry name" value="DEP_DEPDC5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027244">
        <property type="entry name" value="IML1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048255">
        <property type="entry name" value="IML1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13179">
        <property type="entry name" value="DEP DOMAIN CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13179:SF8">
        <property type="entry name" value="GATOR COMPLEX PROTEIN DEPDC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12257">
        <property type="entry name" value="IML1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEP" evidence="6">
        <location>
            <begin position="1186"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="166"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="754"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1267"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="85"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1274"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1292"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="7">
        <location>
            <position position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1591" mass="182093" checksum="97F301F3841C4C22" modified="2017-01-18" version="1">MSNPLTLNIPRRSQVQNALKEQSSNDLRPKLSLNTNRRLGLQDXVSSPLHIQADSVPSSNMNTASITIGKPKQLYQMKTSGIKSKFNKQSSNNTNSNSNLSINISNNNSENHESIPLTNKSYEFELFYHDSRLSTNPLYIDISALPGAKEGDLAELKTYQHKKLSVATDGNKNSPXDKENTVGTSHTSSAMQNLGSPNFTNKNVNMSATKVPKQVDKKIFFMVKDFDKMVKRRXNQTHISMLYGDLQKSLDIPSKSKVWIKLKNKKESQVDLIELNVKDCLINRGDMWLIMNKLNDSCVFNNQKLVFLDTLRMIVSGVYSNGKKLLSGYIGDQTKIIVRSESARFVFVIQITEEMWHFEEDGEIKFHKVVNALFPKIIKKWQKIGTHHNVTIAFSANVDMSTVDFKKSKPGHRQLNSKDYYRVVVDQVPIVHWRTIMETLRKEFMKFKKDLLNVKNEFDDTTKIQGRLTPTVKTNLLESINLATTLLLNPFRQIDLRHTTTHIIIISPGTGMFDVDYELLQLTTKKLLSLEITMDIICLSNPPLHIVPLFRYRDYSDKLHYSIPTWLSISFWNEANKKYKTWSPSCKIYDLQMMGVSENEMEKGISIDSLTPFGQVKNLDEFVTSYDTNIFTDEIWKNVNSHKYSFSSELKEKANNIYKVTPDTKLSGSKFSWQNPKSANPTMHDVSKFQVFADSAKNGKGLSATSEHSSPTLLKSQSSFDALSQALKTKHSQSQMPRFAQRLVSTIFKKPESDTTPMISVPRENEDTKSLTHNISQEDVSEKRESADGTNFLKNNNVPLKKVVASDSDSAIDTATMKNSLVGNSVKHSSNNLTMQQSLGYDSWIEIKNPSTAFDAEMATELIPPRWKDVYPRFVPKRYSKWRSFTTPADLPVTTNLFPSVTDFEENFELRNHTVTLNPDEVLSVPTTFELVRVMIYVRLLCGFQICTSSKIKKIEKSNELNNVSQSTTLQPHNYINKAIYLAMDDEIHRICCRDDSAIEVRQHTQKKDKQKLDHVGSYLPMIKTRYEEEYRKSKIDPLNARRNKSNWNLVDQYLAGYDDNIPEESKKRFKSKFVVIGSDIPETAYAMQAKETLSDEEIRLEGLRKLISNMCRARARVSGEKKDNRKEELLPEIFFYTGHIKQFIEEQEDMLRQHLDTKKNENEGDALMLNKSVDNEKLAFELQWGKDRLDLVTRKWHWNTYHNCFIGSLLVNWILEHFEDIETREDAVSYGESLMSKGLFVHVLNKHGFLDGHYFYQISAPYSTPPKPENQLKENLSXASPKSSNRHRASTIGSRSLTSKKSGSFAPLSAIKSVLSGHESEQSADNIDDKIEVLSEDLIDNNETHTIMLSSKIIIDLDPKKLSSKKELVTVHFDRVHNPMHCFHIRVEWLSTSPKLVDDLINSWSRLSERYGLKLVEIPWNELCSLPSINPFYSHANLKLAIDPWTDDEFKNDTALQTNKFYFHHYLLEHSGFLLDNRGTKIFGNEPSKFEIVYSWGRQSFKLIQYVHKTGAYIAEIGEKGELFLAPNNIHLARVNAITNGTSSDSTKFFLGYQQILLSLKELCLDSTALRSLFLTAKQAFLDAREDSDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGK5</accession>
    <name>A0A1E5RGK5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative DNA-binding protein SNT1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1525</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ85986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGK5">
        <property type="interactions" value="153"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGK5"/>
    <dbReference type="InParanoid" id="A0A1E5RGK5"/>
    <dbReference type="OrthoDB" id="10258692at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034967">
        <property type="term" value="C:Set3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051571">
        <property type="entry name" value="N-CoR_corepressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13992">
        <property type="entry name" value="NUCLEAR RECEPTOR CO-REPRESSOR RELATED NCOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13992:SF39">
        <property type="entry name" value="SMRTER, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SANT" evidence="2">
        <location>
            <begin position="849"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="395"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="480"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="539"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="725"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="910"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="996"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1110"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1390"/>
            <end position="1450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1566"/>
            <end position="1587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="480"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="725"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="756"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1010"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1390"/>
            <end position="1403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1587" mass="175305" checksum="84FB0554665B80E1" modified="2017-01-18" version="1">MRHFDKKRYHYTGSNTVPLSGANAVNPDTPGRSNSNVSNNSNLAHFKHNHPRNRTTHLATSAPSASQGPLKYSRFNNTSSSPTPTTANRMADSLSDNKYAHQPYSPNSKARYAAHTAYKSVKPATLSGSNQHNTSKTTPAQQFSSNGSVWSRSTQSSHDSVGKMNSMNQSGTNLQPNLPKHRIPGGSNTYVPSMPKNDEYIPAKVNQEGITHFENSKNSNYHPKNTYRSRFDSSAGSNKNSNRSYAFPSEKTDQIERRFGLQESSSKKSSAQFPENTTTSSKFSLKNNMPMSKKNFKTDNSVPQSKITNRVSKFNHERKNRFGDNFGSDYPTKNEKRMADNQEKEENTISHNSNNLSQNTKDTASSPSSKGSKSHKPSLISSGSVMEANNEHATFSLSTKQKNYNTPKTDSTINGNEYTGGSKFIRSSSVSNALSELQRASDVSDSETDLGFEKNKTDLPSLPVTAHSAFTALDLNTQDKASNTKSTKSSPFGKAQNENAFAKTDHRLSTETANEPLEENENLSTAVQAAKTGNEENMKAELGSKSVGEESQPLKKSSSILFNSDEDKKENEQDFDELLSSTLEGVPEKDFKLFYYDAPFKSTKHIPKQLSFKESPLPVPKVALEECIFPMREVELRLWILKNNPVGIAKQDYRMKTPIRHLNEYPFFYTNIERFYREDKTNIVESLCSVKRYAYLKSLKLRQKYVNQESLWKDKIVSKLDKLTEQHKSKEERNHEKKAQKEEERLAESGSLQANSSLSALDGADSGSSQGGSMSAGTISRRRNRADFVDEEDLESVLLQIDPYYKHHQLAADIPPMLLNPVDKYAKKYQDVNNLVTNKDQWAERIILDGELNFSELERTLFTEGYVQYPKLFSKISNYMGGLRTPEECVIYYYQTKNKCNYKQLLEEKKREKEREKAKRKLLKKMAKGDTSQNTPVKADVSLENGALSESVSAVDLNDKLSVLSERETQKTTNPHDLSGNSAAVNFQNSVGEIPAKPLVPSFEHDSRENQPTSFDGTDVNTHTEGSKSVDSAVLASSFNTNATDPEKATLVSDVLLKKTFLSDQVLDQTSPEANESHHIPKEQTNNVNVALPLAEDTPAGQNESMLIAKKRRSSSGTESAFPKLAKKQKLQNEGTGPGITASSIAKTSYWSVYEANLFPSLLQKHGSNWEAISKDIETKSVTMVKNFYQKRASECGWDKILMNLEDKKTKPDTQNIKSDKNNEKSGLPALFSLETSKQSSVPIQNSLPAVTKPSVQLFAADVQKHVLPKTESANTFFNDTDISLQGLPAPRLPSIQLPSIFPKGESSFNVFGSAQSKQSSAAANNLTSFQLPPIEPHAKTGTELRPIANEIDNGRSASSATPTRKSFDIGNLLNEPEVVHRPVLRTFVSHTNPHSRSGSKSPSPVDPKTFQKNKSPPRLTFLSNILNPSPLGEQSDSKPKTFDNNAQTSLNGSFTNVNNLLNPIKPFVDFGGPFSAPTSSSSSQGAAVSKPTIVSNPAVSQAVLTATIHNPMNSPSLSVTKPHLNVQNANTIMPPKPFDAKPKPAFGNLNFAMDPLAALAAVASSESKLLNEKEDSQSKQQSTPNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGQ3</accession>
    <name>A0A1E5RGQ3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SEC14 cytosolic factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGQ3"/>
    <dbReference type="FunCoup" id="A0A1E5RGQ3">
        <property type="interactions" value="292"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGQ3"/>
    <dbReference type="InParanoid" id="A0A1E5RGQ3"/>
    <dbReference type="OrthoDB" id="1434354at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000011">
        <property type="entry name" value="SEC14 cytosolic factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051026">
        <property type="entry name" value="PI/PC_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657:SF1">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00180">
        <property type="entry name" value="CRETINALDHBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="107"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="309" mass="35469" checksum="0B6DB2ACF2E0F776" modified="2017-01-18" version="1">MSHPTAKELYDSYPHNPSPTALPGTLGKLNAQQETALKELREKALADIESNPATFINKDRLDDCTLLRFLRARKFDVQLAFDMFLACEKWRKENNVDSILTDFHYEEKPIVARYYPQYYHKCDKDGRPVYFEELGSVNLTEMLKITTEERMLKNLVWEYESFQKYRLPACSRKAGYLVETSCTILDLKGISISSAYSVLNYVKEASYIGQNYYPEIMGRFYLINAPFGFSTAFKLFKPFLDPVTVSKIFILGSSYKKDLLKQIEAENLPVKFGGQSEVDEKEGGLLLSDVGPWRDPKYIGIEGEAPRAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGX4</accession>
    <name>A0A1E5RGX4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome b mRNA-processing protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1138</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Essential for the assembly of ubiquinol-cytochrome c reductase. It has a direct effect on the correct occurrence of the Rieske protein, core 4, core 5 and apocytochrome b.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion inner membrane</location>
            <topology evidence="1 10">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the CBP4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGX4"/>
    <dbReference type="FunCoup" id="A0A1E5RGX4">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGX4"/>
    <dbReference type="InParanoid" id="A0A1E5RGX4"/>
    <dbReference type="OrthoDB" id="5576752at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012420">
        <property type="entry name" value="Cbp4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28202">
        <property type="entry name" value="ASSEMBLY FACTOR CBP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28202:SF1">
        <property type="entry name" value="ASSEMBLY FACTOR CBP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07960">
        <property type="entry name" value="CBP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="43"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="123"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="13">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="13">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ86135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="168" mass="20172" checksum="D2E8722CDB91A2E3" modified="2017-01-18" version="1">MSQPGLWMKWAKVWAAGGAIVGAGVLLFEYTVPTKEQLIEQLSPELREQYERERELRQAEQRELMKIVQQTMKSNDPIWKTGSIRSPWERDAFVEDKGDSNNDQPQQQQLVQQQDHFNEFHKIRSDQKQKDDLEKISQELEQIRIKSEERTREILEERRKNAPWYKFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH14</accession>
    <name>A0A1E5RH14_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH14">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RH14"/>
    <dbReference type="OrthoDB" id="4062049at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="377" mass="42669" checksum="47E99F8F87ED46DB" modified="2017-01-18" version="1">MLRGDSXLYVRNASEPIDPENLIPMEDLSKSPSLSVFQQNDRDLMRAYSRSESNVVPGTASLHKLETVSNDFISDPAFFRGQXSDFSSHTNSGNSILNPPFKSXQLDIKGLIDVSAFNFLRKQFMNFXFLFYKCAYFHMYYFPPQHYLSLFXKSKVGIQVAIKGTTFVNGNKVGTIYDETNDNGEHXERQCDEXSNSNKNLTNXQEKCSNSNKNGDKRSAEHKHKIDVRNVIDPKTIHFLNFAKGCSIPSQQAFQRANTRKFFRNTFKKQWMASQEGSDVLHGSSAKKAAIGHSKKGFYLYSVLLVPDRLRFGDEQYAQHIAASVLKINSLDWDKDLRSKVTKANNAVPVAFLNEKLSKAGIDCRVFKRKGGDVHVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH19</accession>
    <name>A0A1E5RH19_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RH19"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="744"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="804"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="899"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="766"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="815"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="837"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="899"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="931"/>
            <end position="942"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="962" mass="108687" checksum="C910A1A7EE1B273E" modified="2017-01-18" version="1">MSLELLRLTSFYSDDLISGIKLLQSKIEKDVDELEYYLQTYDDLXTKVLEPLKQNLHTAFQSSLTSAAAGSSESRTNSSASLNAAGQSKSVAIKGVVSGIKNRILSGDRANIPMHNNTNNRFRASSRGKGLQLTSLKTKDDKSLLYDNGEVPIGIQLNELKLSELXAMYQEFIDKFQEALPLLQEGSMDNASHDEELSANEEKTGAIIELIDDLRLLQTEKIGIILMDCYQGIIDTSKTLNNTVFEYNQKEKSFLKCSESTSEVTKFEANTMVFPLVLIKQLAISNIDQLNQFKEQIHELAKQEMFVIPNSARNISIHSESGVLLTSDEELLILGKKMLEYVXQALNFREIGFTPNIIEIEQLFQNLTSCGITSLYEDTHGSESHGYQAFKYYKFDTKSTAENERIHQNRRSTAICENYDNFINLYISFIKNLHQCMVDYNDVFMRKLMKMNELFGERTEQDXVSIAKPIDYLKEMESLYIANTTTMMSNVGNFQYFGSSLTHLNINLLEKEELGNGKKFSYKECLIEPVLKKLNSDLEKYDGSGNGVGIDGLTLLNIWFKNDLGEVTKILKMNQYLVKMVLQNENLNLEDKIKAKFVAREVNSNQDLNYYHRLVNILKNYLIETELIKYIQGLETTDFSKIIQFMTLRNLQILILLIKHFDLILQKTGYDIDLIQIYLGNGYNNEINTNIVLFSQLFFKNNNFADLKNLNVLIREILFENKYYGIIVQRVEFLERENKFQSSKTVDTDNSDFSYPPLDIKKRPNRQASASTNDDESSASSNTKAFSRSSRMFLSAIQRKLSDSILPDHKTEGSSGTGNNKGTGGAKGHLPSRYRSRSSTASSSLSRTSHPELQEVEDDFEPQVSGSAAHRKTSDISHSSFSYTDDEFVPRAYKRLSSLPLPSESSSPLMTGGTANGSFIGDGNDGHSFISSSTNASNTPSPRHSAQMSRNKKRMSGINLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHF3</accession>
    <name>A0A1E5RHF3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Rho1 guanine nucleotide exchange factor TUS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1232</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHF3">
        <property type="interactions" value="154"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHF3"/>
    <dbReference type="InParanoid" id="A0A1E5RHF3"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052233">
        <property type="entry name" value="Rho-type_GEFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46572">
        <property type="entry name" value="RHO1 GDP-GTP EXCHANGE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46572:SF1">
        <property type="entry name" value="RHO1 GUANINE NUCLEOTIDE EXCHANGE FACTOR TUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="4">
        <location>
            <begin position="559"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="815"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="5">
        <location>
            <begin position="1054"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="109"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="280"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="324"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="913"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="295"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="329"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ86349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1434" mass="163167" checksum="91AF6A1A1135B650" modified="2017-01-18" version="1">MKDANIYGANGGAVREEIVTEDVLKKLPALPPLETLSVNTRFSVNSSQSSSTFMRSEDSTEKFLSPHNSXSSEIIIKQPETLEAVSSSKHKAXYRKQTYVVEDQYSNKLNKSPSVKSSVSVFNPSKVTPGFRPKPPLIDRPTSPLSPTSRTPATPSHSSNARKKHAYXKNIMGSVNETLSSSPNXMKKIPLSTKPAKHHLNLPNRSSXTKEVLPPRTESIVSSLDCISPVVTTGVQTADLIEFLHEEQHCKTDQKLLPTKSSSEEDISCSPNTVKFKKPHYPVHVLQKPPPPVPSRQTSNTYSSESNPDYLQSRYSSSTYTFSQTTPTVKTSSNGDTRNTSVNSLMGNRPLHQVPSVTIPTNPFNIDHLDEGRLNACVNVYQLSSIYEWLLKIYFEWFSDYIFYKFDFFELVQLVLEHKLTKSYNQETIDSNVDRIIEELIFQKAVRFEEDIANPSASNTDVDLQTEELTIIAPGINVSGVLANLLPCGSCSSTTSKYPCYSFLCRTQSSITNLQKTNNTLENSELKRPQLSKKTIGAWSDYWKLTAEDLNEISPKEVKRQCFIFDLIILEERSLNLGNAAVEIYGKNFHSSLLPNDLNFFQLAFEIFLPLINVHSNYLLNPLYNKLETKGKFIDSVGKIYLKWVHEATDIYVQYAENMATVNEIINWEKQQPHSKFSDWLVQMDNSPEISRSKIYHDVIFFGGFFKSLQNMPITLSSILKNTDRSLEDYDYLKMAIEEIENLNSLVDKTMGACSDRRRIIRLSKQLVVSKNIMHTNMRQSHSALDASSAKTESSTSTFAGTDDLNLRLNEKSRVLLKEGSLLKKRELWVDSLPVFVLLFDNYLLITEPIIQNNERKYKLIERPIPIDYLSLETKNKELEQHLPEKSRPSLQHTTSGTMVYSLQQTMGSSRNNANLEYTTKDPQQEQQTPKHSFSDVRRANFHPGVFTDGANGNNSQMSFKLRNTATNDSYTLIATTYDDYMVWMNAIVQALKNHNSDKNSSNVFKLECLNDMFSYHDKSAPTTVPVSAEGSPLDSALKNYYAKRALPTTPNLKADLSCFVKFKYETRVFVLCAVSQGIFMAELNKNTLNNHNNWKFVLRLEKIEKLEVSVSMDLLFVLFDKKLVYFSLPSIILSFYDSAKYLHNNEIVSIVISEKVGNFCLAENFENSKQLIYEKRGYVYFTTPEYDRISKIVRFFKFYKAYKLPSASRITASEREDIKVFTKSFIVSNAXKGFLLFNDSFNDEGLQLPIFPKDIKCDEIRNNPKISSSLNNGADFDHFNGDILPNDAERTLKTQERITQGVINDIASNKTNPITCFQLKNGDFILIYDEAVVKMNKNGQVVNGQKDVLVLDFYCNDCSFNNEFLILCGENLIQIYDFSTEHINDTFLHRLIPVQIVKGKRIKFLSCQRTDDTVISLSHPWIIGRQLILGFYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHI3</accession>
    <name>A0A1E5RHI3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1008</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHI3"/>
    <dbReference type="STRING" id="56408.A0A1E5RHI3"/>
    <dbReference type="InParanoid" id="A0A1E5RHI3"/>
    <dbReference type="OrthoDB" id="2399539at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050987">
        <property type="entry name" value="AtrR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910:SF37">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="8">
        <location>
            <begin position="450"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="44"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="141"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="220"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="44"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="141"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="840" mass="93300" checksum="9C248E8CDA1CF6B0" modified="2017-01-18" version="1">MKKEIRDFKKQEFKDILEHLDALEKKVETLLDVYVNSPMNSLNSADAASGSTDDNPQTVGPCSGDTLAPGSTIRLETKVKSKSPSFPTSNLDDTPLGDSNADDTTVGGICATNASEKPSVSDASTSPLFLLANAVTSSASAASNFSPQSSKSSGINNSGFSNGTGNLNRSSSTTLLAKRGLHETEQNGLNSNIPESDAKRLQLLQNTSTADANRFGLNVADNGGDHNSTFPKRASGGSDSASAEHDPTFFNINTDGSTATVGSAHSFTSSSSPNLSTIFFKPLSANKDYVWSQIRKGIPEKFYQEFDTLDLKIIENNKAKQEIVNKMIENPKIEEIPDIKIVKELLNISINKLKLNLVIPIEDYDVIGILQKWKTEGYNSLNNSYLLILNVLCLMSCEILLNFLQSGDDAVSVSSGQNGACGMDSKCITAGIVNQELLDTEWLIKNEEIFLGNSLLYCQSISLFPDGLASVQGILALLIYLPVIGVNLGNALLLSTAVRIAQDLGLHSELFSEAIKNNKRLREKKIRIWWFCCFIDEMISTSLMKPHLILKYNTTVTFKARNKAALLDIADEHCRKTKKNGGGFSMNGVDIDDNGSKINNSINNDEYLQACEVRENLIKYVYSSDGITPILRHYAFKFSQVFFQNSGRFQTSTPADIYKDVMLQISECFEDLPNLIKNHEMIDNNFPQEESFFIYILNLYFIEAQNTCSQGLYDLNNEKYVEFLKGLLKLNLKLSKFQTFYLFQEMDCMYFINFIKVIEYNINFEKNLQTFMENYQFLRDFVSNIFNVPLTNDGSLGESIYVKNDWRRAKYELLKRILSIMKEVGDMRAASAASAASAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHN8</accession>
    <name>A0A1E5RHN8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHN8"/>
    <dbReference type="InParanoid" id="A0A1E5RHN8"/>
    <dbReference type="OrthoDB" id="4063132at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018304">
        <property type="entry name" value="Rtt102"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09510">
        <property type="entry name" value="Rtt102p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="313" mass="34046" checksum="D430C21E7B626A7D" modified="2017-01-18" version="1">MAESNIQSTEKRGVERKSFLHDPILASASGYVGNAVNNKAWTQEMYYPMRTHPFSNNAAPDSIGAKNGPDSTRYAFKISSWVEVPVKNTIESQDMMDIDTPKDFYNLEKFDRFKKDQAQKDALSMADIRGAVGTGGDSIPGLTASTNAQQKEETNSTPVEQHVNMDEEVAVNHGEPLEKEDISAALTGEPQTGAVEGSTYEDKPDEVPVKASGQENVDLGKGEMQKELAGQSEPQDNLESQTPAASSAEPIESLAEPEQLQSQNDQTFLQSTTEQSPVEQSANEQPTIEQPLTEQPSEGTQETNSEENTGTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHS7</accession>
    <name>A0A1E5RHS7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Macro-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1442</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHS7"/>
    <dbReference type="FunCoup" id="A0A1E5RHS7">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHS7"/>
    <dbReference type="InParanoid" id="A0A1E5RHS7"/>
    <dbReference type="OrthoDB" id="6082470at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028071">
        <property type="entry name" value="Macro-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14519">
        <property type="entry name" value="Macro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro-like" evidence="1">
        <location>
            <begin position="6"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="279" mass="31526" checksum="676E4AA9DFECD513" modified="2017-01-18" version="1">MIEIILNSSSKELTSEFNKLITKYTPKFRLFRVQVVHAKLSPTIFQRKKTTQNAVRSKASVEKICIVSPGNSFGFLNGGFDKGIMNLFNSCNSTQDFQALFQKELRMINKTSYSPVLNPTLVDLAKYNYTCELLDENAPGGSICMQYTHILHLPTMVSPEQPLFPKKSGNATNNNQKQLSFVFDCCFRSLLQLRDTFFKIDCIVLPGFGAGYGGVEPANCALGILLGIKLFEIDHLKLVDQPTLQKYLLQLHGYKDVNIFQENKKIEPNKVDLEKILKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHW9</accession>
    <name>A0A1E5RHW9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1417</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHW9"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="385"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="458"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="580"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="463"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="484"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="522"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="555"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="580"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="600"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="806" mass="88376" checksum="BB9511D7AA004575" modified="2017-01-18" version="1">MPIQIAPKPASVHYQPIKPLLNVNTTTTNTNTNTNSTGSSSSSNSNIHTRKLKISPYNQQHKKLSLSGRFKKSASTGIVVRTSKKWEFNSSTHHNTNGVSNATKGVSNATNKVNGKSQTSKVSEKAQTNKVNEKAHTNKVNNKDSNNNTTNDTIKSCTIVNKPISKINDTTTSKVSAISTSEVTTPTTSTPSPHSNNTSNSNGVPKIDYKPSSTNTTIPKVVDVESNKKTEXADVQKIQEKNIQIAPINDVGKKCLXTTSDQTQLVQQNKTDTATATANATATATATATATATATATANANANANANAPLITTVTENTNKTATKQETKPMNKRLLKKDINAMKTENINLKKELGGLIESLQSLKHQFNVINQQIYQKQLASTXDTTTSHSINNSKTTHQAQDLNRKRSYMDVDDSNVYGHIIPVKNDYNNVFDEPNKENPAFTEQLTDIFLKFEDENDFPLTSDKKPEXTEANNHETEQDHESSGNTPNSLFSVYPSTSSSLNTTGMNSATNINSTKHHPTLNVNTTNDAKTNSNVPKSRPHSGNHSPATSHNTHYSASNCSPSSSVSSLFQISNNVSATASVSGGTSFSRSTSMMNVDTSSSATATTNNNNNNNNNNNNNSHHHHYHSLSSHPQMKFLNDFEEKEFMKKYAHLNQTHHRHSVPQPRLHAKPHVHQLQLQSTTTSLGSSPTTHFQSDNENENENELLIDAQKHVLENIKEEQEFMTAGLSKNLTSLTSFTNLTNLTNFEMMNEEKSLGFYNTKDTGLLAFDTANDDIQMSLEDVLGDVVDLHAHDEDDIDMDFLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI20</accession>
    <name>A0A1E5RI20_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="13">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g1175</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) + 4 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 4 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56008"/>
            <dbReference type="Rhea" id="RHEA-COMP:14356"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:139493"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) + 3 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 3 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56028"/>
            <dbReference type="Rhea" id="RHEA-COMP:14358"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:60628"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="13"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RI20">
        <property type="interactions" value="870"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI20"/>
    <dbReference type="InParanoid" id="A0A1E5RI20"/>
    <dbReference type="OrthoDB" id="8118055at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF55">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MANNOSYL-OLIGOSACCHARIDE 1,2-ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="30"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="366"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="497"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="543"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="622"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="439"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ86526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="638" mass="73379" checksum="0C7061E86F26FE12" modified="2017-01-18" version="1">MGKGDKIRKRGSPETTKDQDPTVGETSGYSTKHNNHNITNXNSKKRTQNKTKKSRSGDKKLPQQSLKKRPTGPLSRRFAGRINNGNRKWWSLGIILFMFFMKYLQKYNESSKPLPFKSASNAKLFVENTFLDSWGHYVEDAFGYDMYHPLNPVGERGSNMLSNEKPLGWFIVDSVDSLMVMYNNTESNEHKVQFGKEIERCEKWIDSKLDYDIDHDNLNVFETTIRMLGGLLGAYYMSNTYHIGNSSVYLNKAIDLGTRLSYVYSNGKSIPLGKINLHSGSSSLEGNSRTSSLAEFTTLQLEFKYLHTLRPDLFPQLWEQSEACYDVIEANNDLYGPRWQGLVPLFIDPVNGQFKGQTIRLGSRADSYYEYLIKQYIFSPTNQTEHYHRFIKSMNGVKNNLLGQTPKRRGKHNGGLVFISEKNHGLSGPLSSKFDHLVCFIGGSLMLGATKGVPYKEAKEQEWWXEQCASDWKLGLEITRTCYNMYSENDVTGLAPEIAVFNSNHPEKFEELKDPNLASGWWSSRNNDFFIKPNDAHNLQRPETVESIFYAYQFTKSQTFRKWNWSILKSFIRSGSICLQDVTREDDNKMNNVESFWFSETLKYIYMTFLDDVDLSSIVFNTEAHIFPTLKSPNELDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI39</accession>
    <name>A0A1E5RI39_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Vacuolar protein sorting-associated protein VTA1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1604</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the VTA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI39"/>
    <dbReference type="FunCoup" id="A0A1E5RI39">
        <property type="interactions" value="455"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI39"/>
    <dbReference type="InParanoid" id="A0A1E5RI39"/>
    <dbReference type="OrthoDB" id="391137at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.270">
        <property type="entry name" value="Vacuolar protein sorting-associated protein vta1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044538">
        <property type="entry name" value="Vta1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039431">
        <property type="entry name" value="Vta1/CALS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023175">
        <property type="entry name" value="Vta1/CALS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041212">
        <property type="entry name" value="Vta1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46009">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VTA1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46009:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VTA1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04652">
        <property type="entry name" value="Vta1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18097">
        <property type="entry name" value="Vta1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vta1/callose synthase N-terminal" evidence="10">
        <location>
            <begin position="9"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Vta1 C-terminal" evidence="11">
        <location>
            <begin position="307"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="189"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="196"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="206"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="285"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ86253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="351" mass="39451" checksum="CFEC0CDBE1966F48" modified="2017-01-18" version="1">MSVQDLKYVSRLLKEISDLDTQELVFVSYYMKLYVIEIILKRGIEDYKDRAIGFLDDVEGLKKTVDDSANEDEGAHAIVHDLEGKGKIYVLNFTVTLYNSLLKKQVDLEKSLDENMGHGGLGPVNSLISGFWCCIDLFAMVQETWLKEDIEAAKEVGKKTQFGKLMIKKLLVKKKCLIDKIGADEISGEIQDGQEKPQSLKSTSISEEKNNSVSEFKPENDNQEDVPENDNKEDVPDFVDEAPETSPEPALGLPSAPKFLDDSDKDELQETEIDAKQEIPTPSQAHEEHSKPLSEQEIMAMMDWATLITKAQKHSKFGISAMNYDDVDTAVTELQQALDILKILKTKESQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN33</accession>
    <name>A0A1E5RN33_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Conserved oligomeric Golgi complex subunit 6</fullName>
            <shortName evidence="10">COG complex subunit 6</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 10">Component of oligomeric Golgi complex 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g841</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Acts as a component of the peripheral membrane COG complex that is involved in intra-Golgi protein trafficking. COG is located at the cis-Golgi, and regulates tethering of retrograde intra-Golgi vesicles and possibly a number of other membrane trafficking events.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Acts as component of the peripheral membrane COG complex that is involved in intra-Golgi protein trafficking. COG is located at the cis-Golgi, and regulates tethering of retrograde intra-Golgi vesicles and possibly a number of other membrane trafficking events.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the conserved oligomeric Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Golgi apparatus membrane</location>
            <topology evidence="1 10">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the COG6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN33"/>
    <dbReference type="FunCoup" id="A0A1E5RN33">
        <property type="interactions" value="274"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN33"/>
    <dbReference type="InParanoid" id="A0A1E5RN33"/>
    <dbReference type="OrthoDB" id="272987at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010490">
        <property type="entry name" value="COG6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048369">
        <property type="entry name" value="COG6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048368">
        <property type="entry name" value="COG6_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21506">
        <property type="entry name" value="COMPONENT OF OLIGOMERIC GOLGI COMPLEX 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21506:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20653">
        <property type="entry name" value="COG6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06419">
        <property type="entry name" value="COG6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01087">
        <property type="entry name" value="COG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Conserved oligomeric complex COG6 N-terminal" evidence="12">
        <location>
            <begin position="275"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Conserved Oligomeric Golgi complex subunit 6 C-terminal" evidence="13">
        <location>
            <begin position="405"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="92"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF06419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ88298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="908" mass="103882" checksum="5038E4B37E7286E3" modified="2017-01-18" version="1">MDFLDYQTFITTDTSTDDNASLNNSSSTVMKSVLPEPAKRLVQAEGAEQNYGGSVFGTSAAAMATKHLNEANFANALTNQFKRMASPYLNSENETQNSELQDQNQPEPSLSENGASPHAYSAQNIGGPETDQKMNKYLDISIKFLESANTTAPVDRSTLTSNNQPIKLPNSEYSSSFNSQFNAASQKSRANTSTKVNHSEQILASKISQILNDSSLNNASVEVQPNNFRKSLTLLQSFIYDSTTTASSAFLDYDSPNTLFEVNEILRTDFVGVLQRKAMKPKLEQTILKEHEYFLQQYAPIIEKLKIVQPSVARLLDLKKEIQSLPQNSINPTVDVLQTEIHDLKLEKKVLIGLKGLFTLDQLEDDILHNGKIDQDFFDVINKLLLIKKRCVFILSETNENFNVLLNKINSDLQLATRKIYNSLMDFLYHFDFSTINASKNTNSTVGNNEVLNFQKKLIYLSNDLEYFNSFLTRIVEIRSKKCLDNLFAQFDLNNTKKPIVLTAHDPVRYLADVLAYVHSLIVNEDDFVNSLLKFQHIISEDTPKTILQENSSFLSGLDSKILNDIFIKLSNTIKIRLEQIIRFENNSLKNLEIIQLLKLYHMMYTKYGILEENVLLKNLKELSNISKDKIFQDFLDFTTNQLQKVFDISNADLLPPDWLPKYLNKLTDFLLKLETDTAIFDDDNMNKMAEELVKTPLTTLINQQIVKSYPMAKRDPQVKTAMLIIKSNCYDLIKTRLLPFHHTIFNINDSLQNVYDSICKELVVCTKFLEELQNKTLLINTGLENYFNLFNMVFPVASVQDELDYEMYYSIKENKIMNLPTIETAVSENISDYLPRAMSDFEERLLFHLSSPVIADEITKNCFNNFSQFFRVFFHTLLLLYPEDKDLIKKIMVFNPEEVDTLIGVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN36</accession>
    <name>A0A1E5RN36_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein RTM1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g898</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lipid-translocating exporter (LTE) (TC 9.A.26.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN36"/>
    <dbReference type="FunCoup" id="A0A1E5RN36">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN36"/>
    <dbReference type="InParanoid" id="A0A1E5RN36"/>
    <dbReference type="OrthoDB" id="3358017at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007568">
        <property type="entry name" value="RTA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465">
        <property type="entry name" value="PROTEIN RTA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465:SF1">
        <property type="entry name" value="PROTEIN RTA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04479">
        <property type="entry name" value="RTA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="343" mass="38849" checksum="6D4B4C46648EE7EB" modified="2017-01-18" version="1">MSSTTATQSTSTATSIANASASVASDDYDFYDYNPSKILTYVAFILFGMWTLIICIEIFVKYLKTLDLFKTTKAYDLNRKQLKKTINAVCLHIYIPLILGGLMEMGGYIGRLDSIYDTSSITAFVVETVLILIAPVMISATTYMLFHRLLRYLNVPENSRVGIFMKNSLMVKFFVAGDVISFFLQAAGGGMTAEDSTMHVGEKLVIIGLVIQLVSFGVFLFVQIHFTLRYKKFCKDFYHLKVTKKWKYINCCLLIASVFIFIRSIVRVAEFGQGFDGYIASHEVFLYVFDGLLMLLTLVVLSVSFFFTDIGEIYFQHFTLTYAQNEENDGLFPYKTETNDSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN53</accession>
    <name>A0A1E5RN53_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="4 15">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g933</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Endoplasmic reticulum membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="4 15"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN53"/>
    <dbReference type="FunCoup" id="A0A1E5RN53">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN53"/>
    <dbReference type="InParanoid" id="A0A1E5RN53"/>
    <dbReference type="OrthoDB" id="292747at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23283">
        <property type="entry name" value="beta-trefoil_MIR_PMT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF50">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 15 18" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="614"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="659"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="692"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="717"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="17">
        <location>
            <begin position="356"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="17">
        <location>
            <begin position="421"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="17">
        <location>
            <begin position="491"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="890"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="17"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="893"/>
            <end position="903"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ88316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="956" mass="107645" checksum="4BD2AF8D60A5E173" modified="2017-01-18" version="1">MAKKSAVTSKPKEKQVASKAKSSSPSPASTLEKEEAGTSVFSLDYDPIENYNIEKGPVRQYLSVDVSDTLSKLRNPNTTVEWIVLSLLLVLTCVVRLYGLSYPDSVVFDEVHFGKFAAKYIKGTYFFDVHPPLSKMLFAGIGSLAGFKGNFNFENIGDFFPKDVPYYALRLFSATCGVLTAIFMYLTLRCSGVKPWIGLLATSLFVVENSFVTISRYILLDAPLILFISGAAYAYKKYEIYPEDSFKSFKSLVFTGVCLGMALSSKWVGLFTVAWIGLLCALKLWFYIGDLNKPLVKTTTIATKWGSILLGLPIVIYMLSFSIHFKALNTDSEGASILSPAYRASLDGNHVPNNIIGPVGFGSVISVRHINTNGGYLHSHNLFYEEGSKQQQITLYPHLDGNNDWLVEDYNNTNVENYTSFEQLKHGTKIRLKHINTQKRLHSHDHKPPVSHQADWQKEASAYGFENFEGDPNDDFIVEIDRKLSKNGEAQEVVKAIDTKFRLRHAMSGCLLFSHDTHLPDGGQQEVTCAHSGIPELTVWYIEANTHPLLANDPKVERVSYPKFGFWEKFVESNIKMWKVNNGFTESHVYQSDPTTWPFLTRGIAYWGKDHRSVYLLGNAVVWWLSTATVAVFAGLVLFELVKWQLGYELSSDKAVINFHIQITHYLLGWFIHYAPFFIMGRQLFLHHYLPAYYFLIMAFAHLLDLFVSHILSRKKIIGLSICVALLSSTYYFFETHKGFGYGTPLLKETCEKGKWMSGWDYSCDAYLESWDEYSNYTLSSEGPKQTSFQATNINELFKDDGSGNKQFDSNLVFEQDKRLAELNNQQQDQQPYDHQIANSAGPAVEVGVEDVKKNQGPKKFIGPDGQEIDEEVALQMIAEQGGRLMKVEETSTKGGATQSAAPTQKYPDVGIDDIMKDEGPKKFMDQFGNELDPEVAMGYLNQGGHIVNVERHGQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN66</accession>
    <name>A0A1E5RN66_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Chorismate synthase</fullName>
            <ecNumber evidence="3 7">4.2.3.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g886</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21020"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="EC" id="4.2.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>FMNH2</name>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
        </cofactor>
        <text evidence="7">Reduced FMN (FMNH(2)).</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the chorismate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.5" evidence="3 7"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN66"/>
    <dbReference type="FunCoup" id="A0A1E5RN66">
        <property type="interactions" value="257"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN66"/>
    <dbReference type="InParanoid" id="A0A1E5RN66"/>
    <dbReference type="OrthoDB" id="1721239at2759"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00090"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004107">
        <property type="term" value="F:chorismate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07304">
        <property type="entry name" value="Chorismate_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.150.10:FF:000006">
        <property type="entry name" value="Chorismate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.150.10:FF:000009">
        <property type="entry name" value="Chorismate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.150.10">
        <property type="entry name" value="Chorismate synthase AroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00300">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000453">
        <property type="entry name" value="Chorismate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035904">
        <property type="entry name" value="Chorismate_synth_AroC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020541">
        <property type="entry name" value="Chorismate_synthase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00033">
        <property type="entry name" value="aroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003793">
        <property type="entry name" value="PRK05382.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21085">
        <property type="entry name" value="CHORISMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21085:SF0">
        <property type="entry name" value="CHORISMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01264">
        <property type="entry name" value="Chorismate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001456">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103263">
        <property type="entry name" value="Chorismate synthase, AroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00787">
        <property type="entry name" value="CHORISMATE_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00789">
        <property type="entry name" value="CHORISMATE_SYNTHASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ88327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="376" mass="40767" checksum="6EB618EE6A989F67" modified="2017-01-18" version="1">MSTFGRIFKVTTYGESHCKSVGCIVDGVPPNMPLTELDLQPQLTRRRPGQSKLTTPRNEKDKVEIQSGTEFGKTLGTPIAMIVKNEDQRPHDYSDMDNYPRPSHADFTYMEKYQTKASSGGGRSSARETIGRVAASGIAEKLLSKFSNMEIVAFTSQVGDVSMNKNPLDPTFQKILQTITREKVDACGPIRCPDPKLAGEMVKVIEKYRGDHDSIGGVVTCVIRNVPTGLGEPCFDKLEAVLAHAMLSIPASKGFEIGSGFQGVNLPGSKHNDPFYLDEETGKLRTKTNNSGGIQGGISNGENIYFSVPFKSAATISQEQETATYDGENGVLAAKGRHDPCVVPRAIPIVEAMAALVMADALLIQKSREFSSKVFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNC7</accession>
    <name>A0A1E5RNC7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Chromatin modification-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g692</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of an histone acetyltransferase complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of an histone acetyltransferase complex. Interacts with H3K4me3 and to a lesser extent with H3K4me2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The PHD-type zinc finger mediates the binding to H3K4me3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ING family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNC7"/>
    <dbReference type="FunCoup" id="A0A1E5RNC7">
        <property type="interactions" value="308"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNC7"/>
    <dbReference type="InParanoid" id="A0A1E5RNC7"/>
    <dbReference type="OrthoDB" id="5411773at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15505">
        <property type="entry name" value="PHD_ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028651">
        <property type="entry name" value="ING_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024610">
        <property type="entry name" value="ING_N_histone-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333:SF42">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12998">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01408">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="13">
        <location>
            <begin position="403"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="143"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="187"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="368"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="190"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="214"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="249"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="262"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="368"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="386"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="406"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="419"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="424"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="430"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="433"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="446"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="449"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="405"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="416"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="420"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ88384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="459" mass="50796" checksum="CF93B166BCE5C13C" modified="2017-01-18" version="1">MTPSKQPVNLYPGLNDISDVLEELPLNYSKYMTLWKEIDAKCVNSLPFLNTSITDFLQTLDSNTATADQLLTINHVFQELMPSIEEKLHVSSIALESIEQIMQRMELAYEVCIKNEEIPVKLRLGNNNHPAMHLHYELMQKAEKEASSMNANGSTSHAHERRGNTRSAAAAAAAAAAAAAASAASAAAGGTSSPGTVQTNGQNATGRDPRSPGGSFTNVNGLNGTVEDKKTRKRRNKNNDNDLQDQNGKKKRERKKRAILVTTVSGDGNGNSSSSLVSKRVKNSSSSSLINSNLGNTTTSNTSNTNNTTNSNNNNNNNNNNNDNGSSNSTKRQYNRSRSSHSTPTQDSTDHKSGKLNETDKAHIHNHEHFRAEEDVGNDEYHNDHEEEEEEEEEEEEEEEEEERYCFCRGVAYGDMIACDYKDCKYEWFHLPCIGLTKLPKGKWYCKFCAPLVSNNNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RND7</accession>
    <name>A0A1E5RND7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Bile pigment transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g660</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Cooperates for the ATP-dependent vacuolar transport of bilirubin and glutathione conjugates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RND7">
        <property type="interactions" value="163"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RND7"/>
    <dbReference type="InParanoid" id="A0A1E5RND7"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18579">
        <property type="entry name" value="ABC_6TM_ABCC_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000565">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000020">
        <property type="entry name" value="ABC metal ion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000013">
        <property type="entry name" value="ABC transporter C family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000997">
        <property type="entry name" value="Multidrug resistance-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044746">
        <property type="entry name" value="ABCC_6TM_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF443">
        <property type="entry name" value="MULTIDRUG-RESISTANCE LIKE PROTEIN 1, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="185"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="366"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="449"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="474"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="548"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="587"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1031"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1082"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1169"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1274"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1300"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="14">
        <location>
            <begin position="339"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="671"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="14">
        <location>
            <begin position="1037"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="1367"/>
            <end position="1627"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="916"/>
            <end position="943"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ88398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1634" mass="185384" checksum="B75458B1D418BCC0" modified="2017-01-18" version="1">MQGNSTLPSSLFFDGLTHGCKYGLFQPYPSRDVNVLNPCFISVIQSVLTSVFILTGAHELWKLYNIKIVPPQFKYQNSKLRSITPKHLTQLSNVAFFIILQLLQYSLFKDEIRNITKLSVFLNLLYLIFLNAPQQLFQFFKTSAASTTSLFYYLTSSFIYGFVIGQRYFHPTEQKYNILPLNSEAAILEIVIFIISLSNFIYEVALYEPSTVLTEYFKEVNYYPPVNFFESITFTWMNKFVSDVYKAGKVEDPYNMPLPPCNLSVKDCNDKLSAAWEQQRWNASMSSSSSAVQSSSVFSIFSKNKKNVKTVKPSLLKAILKVFGKTIFVAMCYETTNDIIRAVQPRFLQQFIKTFETSDSQSQYPVLNAFFIAILLFLIVVVETVLTNQFFIIIFEAGLGIRSALMTMLYQKSLKLSVASKERKTTGDILNLISVDVLKLQRFFEDCQSIVGVPISFIIVLLSLYNLMGYAMVGGIVAMAIMIPINSYISTNVSGLFKKNMQYKDMRSKLITEILNSIKSIKLYAWEKPMLSKLSHVRNDLEMRNYKILSIYNCLIMFCWNLVPVFVSSSSFALYSHFNEKPLTADIIFPALTLFDLLNDTIYYLPNIISQVIETRVSLNRLQDFLDYEETDDSFIFHEDQPKDGASALPTVEIKNATFLWKSEASIKAEAETQNEQNKSDEEEQASSLKYALKNIDDFKADDGQLTCIVGRVGTGKSTFLQALLGSLPAIAADPLKSGPKLIYRGKSIAYCPQQAWITNSTFKENIVFGYRYDEQIYQATLKACQLLPDLKILPDGDATVVGEKGISLSGGQKARLALARAVYSRADIYLLDDVLSAVDAEVSKNIIEQVLSRETGLLKNKTIILTTNSVKVLKHSGSVYALQDCQIVEHSSYKDIMQKQDGSLLKKLVEEFDTFESVQNRQVKEEETLEQKEQRVEIIEQKMDVNHSQEDEDDDIAQNPDDFNPLEIVKSGGAPGNNNPMIETRSLLSRRASAATFRPNRLLVEQENDNRKTKQKAENKEEGRVQTRVYVEYAKACGLLGVFLFFFFMFSSNGFELLEKYWLKLWSEVSERVDTDVDHTLLFYVGIYFTISCGASFFAVLRAIFMNLFCSLRASRLLHDRMASSVIRAPMQFFETTPIGRVTNRFTSDVSAVDQGLQFMLNFFFKSVLNYLTTVVVISYVMPWFLVLNTFLLIIYYYYQTYYVTLSRDLKRLTSVSFSPIMSGLSETLVGSTVINAFQHFDRFNFINFESVQFNINCVFNVRSTNRWLSVRLQIIGGLIILFTSVAACASINTSHPISAGLIGLLMAYAMQVSTSLLWIVRMAVQIETNIVSVERILEYCDLKSEKPLIIDEHRVPENWPSEGKIEFKDYTTSYRADLDPSLKKVSVEIKPREKVGIVGRTGAGKSTLTLALFRLLEAMEGKILIDGIDISEIGLTDLRSHLSIIPQDAQAFEGTIRYNVDPFNQYSDEEVMKALELSHLKPHIESMCKAELETSEESQKPNADPQPINKISTDILLKCKVADNGSNLSVGQRQLLCLSRALLNQSKILVLDEATAAVDMETDKIIQETIRSKFNERTILTIAHRIDTVLDSDKILVLDKGEIKEFDHPDKLLEDKSTIFYSLCEKGGYLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNJ6</accession>
    <name>A0A1E5RNJ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g951</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNJ6"/>
    <dbReference type="FunCoup" id="A0A1E5RNJ6">
        <property type="interactions" value="1089"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNJ6"/>
    <dbReference type="InParanoid" id="A0A1E5RNJ6"/>
    <dbReference type="OrthoDB" id="10264538at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000003">
        <property type="entry name" value="60S ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00499">
        <property type="entry name" value="Ribosomal_eL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001380">
        <property type="entry name" value="Ribosomal_eL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018256">
        <property type="entry name" value="Ribosomal_eL13_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01294">
        <property type="entry name" value="Ribosomal_L13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01104">
        <property type="entry name" value="RIBOSOMAL_L13E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ88461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="205" mass="23185" checksum="29FE15E5D32F3043" modified="2017-01-18" version="1">MTRISRSISKNLPLLKNHFRKHWQERVKVHFDQAGKKVSRRNARATKAAKIAPRPLDLLRPVVRAPTVKYNRKVRAGRGFTLAEIKAAGLTAAYARTIGIAVDHRRQNRNTETFEINVQRLKEYLAKIIVFPRNGKSEENVEQVLSTAAAFPIAQPAADVEAREVVVPEQTAFRTLRLARSDKKYVGIRAKRAKDAAEAEAEKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNJ8</accession>
    <name>A0A1E5RNJ8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Vacuolar amino acid transporter 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g863</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNJ8"/>
    <dbReference type="FunCoup" id="A0A1E5RNJ8">
        <property type="interactions" value="241"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNJ8"/>
    <dbReference type="InParanoid" id="A0A1E5RNJ8"/>
    <dbReference type="OrthoDB" id="438545at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061459">
        <property type="term" value="F:L-arginine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005290">
        <property type="term" value="F:L-histidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015189">
        <property type="term" value="F:L-lysine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015194">
        <property type="term" value="F:L-serine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005302">
        <property type="term" value="F:L-tyrosine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF224">
        <property type="entry name" value="VACUOLAR AMINO ACID TRANSPORTER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="371"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="394"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="431"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="4"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ88467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="458" mass="50188" checksum="0DB11B6A364CBE9E" modified="2017-01-18" version="1">MEYTATVLSSSVNLTKTIVGAGLLAIPFAYSKDGIFVGLLLTIIAATATAYGLSLLGKSSKMLDNPRTSSFFTLCSITYPQFTLVFDFAMFVQCFGCALSYLVLVGDVFPSVLGQDREFWIYSSLVIIVPLCLLKNLDSLRYSSMLGLLAIAYIVLFIIIRFFIAFPSLDFQDLGISWFKINSYSGMMSTFSILIFGFTASMNMFSICNELEHNSLKNIDKVIGNSVGVACFLFVVVGTTGYLSFGSDVKGNVILNYNPDSVFTKIGQFSLGSVIILSFPLLFHPLRIATNNMVFSIEQILGKKFAFFKNPMDAVTNTPDNEEQAPLIHRTVTQETTNSLRRQNTASSSLVLDATSFVQEAEAFQVPFTNFRFYIITALLLASLYMLGLNVQGFALVLSLVGATGSTSISFILPGLLGYKLFKNETNSKAIITGSIVCIALGFTVMFVSVFATLYFPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNV2</accession>
    <name>A0A1E5RNV2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA polymerase zeta processivity subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g778</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MAD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNV2"/>
    <dbReference type="FunCoup" id="A0A1E5RNV2">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNV2"/>
    <dbReference type="InParanoid" id="A0A1E5RNV2"/>
    <dbReference type="OrthoDB" id="3973163at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016035">
        <property type="term" value="C:zeta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.10">
        <property type="entry name" value="HORMA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003511">
        <property type="entry name" value="HORMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036570">
        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045091">
        <property type="entry name" value="Mad2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842:SF10">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56019">
        <property type="entry name" value="The spindle assembly checkpoint protein mad2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50815">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HORMA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="248" mass="29039" checksum="19749020CB0276DC" modified="2017-01-18" version="1">MDTLKIYLKCWINLILYYRNIYPATSFALETFNVFNLPINFPINRNPILIDYIENLIQDFLEILKHSQQEHDQNSLDILNEPAINEPSQSPHGCVFTLAIVEIDDTPQTSIVSAHVPFEKFVIDFDEFDFSKFTELEHLESSEIFDECRESLFGLYQFCCKLPKLEDAKYVFKVFIEHAAVMDESVGNSENWLKINQKKNKRLRGENTLEKTSRQFQVQKSGLLGIDLGEELILYEYYQCTQKKAVHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP09</accession>
    <name>A0A1E5RP09_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">H/ACA ribonucleoprotein complex non-core subunit NAF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g729</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP09"/>
    <dbReference type="STRING" id="56408.A0A1E5RP09"/>
    <dbReference type="InParanoid" id="A0A1E5RP09"/>
    <dbReference type="OrthoDB" id="21550at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005732">
        <property type="term" value="C:sno(s)RNA-containing ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000493">
        <property type="term" value="P:box H/ACA snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.230">
        <property type="entry name" value="Probable tRNA pseudouridine synthase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038664">
        <property type="entry name" value="Gar1/Naf1_Cbf5-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007504">
        <property type="entry name" value="H/ACA_rnp_Gar1/Naf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040309">
        <property type="entry name" value="Naf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31633">
        <property type="entry name" value="H/ACA RIBONUCLEOPROTEIN COMPLEX NON-CORE SUBUNIT NAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31633:SF1">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX NON-CORE SUBUNIT NAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04410">
        <property type="entry name" value="Gar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="104"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="351"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="417"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="564"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="16"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="104"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="125"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="150"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="171"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="351"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="376"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="419"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="429"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="564"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="612"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ88625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="633" mass="71543" checksum="F1962A5422606956" modified="2017-01-18" version="1">MEKTESILSTSGSVDLVKEDEHPVATENHQEVENGDQETVQCNGEDTMENASIESESESASVAKAADSSALAPVPGTAQQTSQLPVNIKLEDIAALDDDINADGKKAKEEAKDATDKPGHILTKSSSNSTSHSSSEYESSNTESSSDSDSSSDEDSDDESSNSNKSRTANVEEEEEEEEEEEEEDEPLSGPIRSTNELVEEPIPEIPEDLQVGPTTKVQLIGHIKSCFENNVIIQATTSAEQRVLKDGSILCLEDKTILGPLCEVFGQLQQPFYRVMIPKDKSELLINLKKNEIGKPVFLITQVAHWIDTFELKKLKGSDASNFCDEEVEELEQEFSDDEKEAAYKKQLKEKKNGNKKKPQKIKKNNANNKVKKPQQYDRLKNQNKPDSKSIANAKIPYNYLPQARVPSALKTGVYQSRDSRFSEKNHQNPSQPGPQQTYQQPMVPYNAYMQQQQQQQQPGSQQYSNQYYNYQQYPQYQSSQQNNMAQVNFQQYGQMQQQPSQQYSAVPPQQQMMFQQTPNMQYGQLQYSAQYQPQQTYNQYNAYNQGYGHAQGYENQQITSISYPPQQPQQTNQQMSPQQPSQQQQQQSSQAIPSDIQQVLNLQQYFNHHGSKEGKNKTEPKINDKNSMPDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RPN1</accession>
    <name>A0A1E5RPN1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">37S ribosomal protein S8, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g938</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RPN1"/>
    <dbReference type="FunCoup" id="A0A1E5RPN1">
        <property type="interactions" value="107"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RPN1"/>
    <dbReference type="InParanoid" id="A0A1E5RPN1"/>
    <dbReference type="OrthoDB" id="409928at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.30:FF:000006">
        <property type="entry name" value="40S ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_uS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_uS8_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="Ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ88653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="156" mass="17648" checksum="2DBEAA3AB29B2AB0" modified="2017-01-18" version="1">MSLVNLGNMCCHLQNVTRVRSTLTSVPYSKMNLQISYNLYKHGFLTSLQRGSTKGPDLEFTEVTPDNISTRRLWVGLKYRSNQPVLSKISLISKPNLHIQLNHTDLLKVCKGLTVREIKPLQPGELILVQHKGIVMEINEAIAKKKDNCLVLLRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV91</accession>
    <name>A0A1E5RV91_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein PEA2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g450</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV91"/>
    <dbReference type="InParanoid" id="A0A1E5RV91"/>
    <dbReference type="OrthoDB" id="3996692at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="302"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="642"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="540"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="82"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="652"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="664"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ90851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="715" mass="80398" checksum="1E9EFF4554FE8254" modified="2017-01-18" version="1">MSDTLPYXGVINTDRNSHGNNTKNENNMSSLELMEKCNPELLTKANEYPTSYNALKEALQIDNLGIDQNDTRGNHNTEYADNSNENESFNGNNTMASINTTNIIDHYYEPSAIDIEDSIIYDAHLNRNDDVLMKREDYFLQLKLCTEYAIYKAKKSTMDLSSSFSSMSSPSSQSAGDAGAGLSFGMTAHNNDIPLSKNNASVSNRSSGMLLGKFDYEDDEHFIRNWRYLMKELKNAELLQKELLKNGLKTFEGSQEHAIISNMEKILVLNDRLNNGLLRQLSKISFPDLNVDVQDYLNYSKNKKSKTRSXHDRNSKYRHRNKSNDNFRLLSGSDMRSGLFMPQHQNGLSTRTPSFSGMLDHPIKYPTDGSANTSFPETDTLSISSETEKYQEYQEYQELDGVNNSNMEATSGDDSDTMEKAAVDGVTSYLISEMIKNNLELPISDSSDTYNNXEFLKSCIDALIHKCAAVTESSETLNQNNLGPKDNVKTFIGNTLTGQNPVSSTXSRPVEGENGVAAXKNSLLNSKDHLVEPSSSREVLKQNNNEVNDLKTALKDLQLAHNFLTDKFAKDRFDSTEKIDKLTKKSTKLSNELTEFQTNLQYEQDKYLKIVHQLNEKENTIDMLQSKIDQLEQQALAETALPSPGLPHLXASSDTDSIHSTTSNGSPASTGNSSSYSISLMRQEFKKIVQQMNTKHEIELQREVDKRLKLEKQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVI0</accession>
    <name>A0A1E5RVI0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g515</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVI0"/>
    <dbReference type="InParanoid" id="A0A1E5RVI0"/>
    <dbReference type="OrthoDB" id="3971848at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ90836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="331" mass="35882" checksum="2C1A0A73FE902D58" modified="2017-01-18" version="1">MSTMKEQVFDNTMKNTMNNDNTTNTSKNIPMKPDVVVDSCASSPVNGYSTKNSEFVKRRKSAPSAFLSSSTEEIVNQMEKEQDRMVITLLREIHHLQYENNVYKQKLASFMDVGSMNFGSSGNATPKNTASTSTNTTQSNYNNYNSSYFHTLPTSSSPLGHGSTGTMSNITRQSSLSRQGSIGRQGSISGTVYPSAMHNTGIYPTTSHSSRQSRSRFNSMSNNNGLGVAGVMAQEESPLTSPMPSRRSSWNKQNVGQNYIPGQAVGQGVITSVXPTLGLNRRRSSSSSYNTVSGAVLNGVNGPTDQVSKRGHMIGHQALQHEQEPNTGVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RW21</accession>
    <name>A0A1E5RW21_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Long chronological lifespan protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g361</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LCL2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RW21"/>
    <dbReference type="FunCoup" id="A0A1E5RW21">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RW21"/>
    <dbReference type="InParanoid" id="A0A1E5RW21"/>
    <dbReference type="OrthoDB" id="2234316at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23996">
        <property type="entry name" value="LCL2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034543">
        <property type="entry name" value="LCL2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38425">
        <property type="entry name" value="LONG CHRONOLOGICAL LIFESPAN PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38425:SF1">
        <property type="entry name" value="LONG CHRONOLOGICAL LIFESPAN PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184907" description="Long chronological lifespan protein 2" evidence="4">
        <location>
            <begin position="19"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ90995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="133" mass="15204" checksum="A9679207CA4E7E07" modified="2017-01-18" version="1">MKLTSVLFLTVALPTTFAFFDFGGFQQRQQQQQQQHQQQVSYEDSVLNSQCDGYLCPVTRECVKEKIDCPCEFPKSQLKCILPDKKNYICISKPATSNAKLANLYDDPVQGPQQRIKNMRDCGWVEDAYKGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RW82</accession>
    <name>A0A1E5RW82_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small nuclear ribonucleoprotein Sm D3</fullName>
            <shortName evidence="9">Sm-D3</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">snRNP core protein D3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g296</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the snRNP core protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RW82"/>
    <dbReference type="FunCoup" id="A0A1E5RW82">
        <property type="interactions" value="1194"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RW82"/>
    <dbReference type="InParanoid" id="A0A1E5RW82"/>
    <dbReference type="OrthoDB" id="6425924at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01721">
        <property type="entry name" value="Sm_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000002">
        <property type="entry name" value="Small nuclear ribonucleoprotein Sm D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027141">
        <property type="entry name" value="LSm4/Sm_D1/D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034099">
        <property type="entry name" value="SmD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23338">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9 12" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="11">
        <location>
            <begin position="7"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="96"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ90963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="106" mass="11839" checksum="0FBA34AB6D733EBB" modified="2017-01-18" version="1">MSNSNSIPIKLLNETQGHVVSVELTTGETYRGKLVESEDNMNMQLKDCVAVGRDGKETTTMSNVFVRGSNIKFIVVPDLLQHAPMFKKKSTLQSKPIPPIRGPRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RYS5</accession>
    <name>A0A1E5RYS5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ribonuclease P protein component, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g258</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000002">
        <property type="protein sequence ID" value="OEJ92132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RYS5">
        <property type="interactions" value="281"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RYS5"/>
    <dbReference type="InParanoid" id="A0A1E5RYS5"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013888">
        <property type="entry name" value="RNase_P_Rpm2_mt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08579">
        <property type="entry name" value="RPM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="438"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="268"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1512" mass="173351" checksum="E2EAE551F20051C8" modified="2017-01-18" version="1">MQFRNFKFCSKSYTTTSTSTATAHVNVATGSKSTNTTTGCNTGSSTTSTASRHLIDNKSDFFESNTTTKKTNNSFNNTRQNKNPYGRRNNSNNKKNNNLISFTSNLHPSAHQVLNPSIRSNSYQSVKRCPAFHRSFRFGRGRHRNFRNNFYSNYNYKYCYEDTQDNGNNNKNTNNKTSNAYNNNRSTYSANSSSSFRGLNAVGANINYKNIDQQLDINNYNCKIIHRRNSIDIMNDIAQNTCGLNTKLDSSIGNRMNNASELISGNNTDTTTTATTTTTPGNSLKSTRMGVXNSQLTSTSSLFPMTLSESSTAXSVDVNQQRIPNTDNKPKFRPRSQSINTGSIAYNINTKQKHLPILSQHNHELLNVDPNVYLGHXLNNNFSLNASKRYLSTMGRKDGIVXSYDNEFLLKNKTADEPHIINIIDSGDLVSQQKKQPSNXVLDHQVPNTSKTSPWPLDTDDTLNKEKFLDQNIKEIDLCFTEGKFNKINSLYMALKRNDIVPPLPVFEKIIASLCLRDLDSQNLDERMFNLLNCYQDLINSKKYHPSSMIYDHVIATLLEGSIAAKKLNNYNGMDFFKIAIELFKTVQKQSLSNPSQTLLTNLLVSVNLYPGYISFNELYAKVAPLVSSKDHLYYITLLKYCRDPAIIDSLIDEYNNFNLNSSATSSDDSNKYKVLSIAIESLMKTNNLTKASKILDETIMEIKNLNGQSANIQLLLSKYLISLSASKDNYKKAYKMWYKFSKLSWIPPFTYDFYLQFLSNVLNNNDWDLACRLYYYMYTTTNEASEYQSDSTLKNAESKTGNTFAGSFALTSNSFKKTSQMMEDEMVDVLLHPSNVDSLHSHFLNMALKKKSSKLILTILEESMLKNYKFDLPLYNKIFEHLKNYSKTHQDYLMRFVECHGSLLESTSNYEKFEFLNSIVKNYQNQTLLNDISKMKFFMKYCQNFKVINNFNYTGLIAIFENSWKSPQSIMLSSYNLELHAYIVSEFYNLDYHFENPAMMAQINSSNKVLAQFKEDATNKFKRLIINYKRLNLDPSALSERVVHLATRILDIPEVDASDFVLRGDWDKTYPVHLGSKIRNATKQGLKDYQVLQRKGYCFDWDTYYTLMTKNVIDANIVTKLITEAPTFQQLHDSMNIMISLINPVNLNNYVFQHELFTTKILPNLNDKSLSKIVKVVPDLNKFLENIDFATHFKPIKQQIDFENFISMIYTRLFQEKRYNKILIMNKKVPVLNLSILLKCYLRLGDIALFEKYLAKWGQKLSTNELNDIKAEHLVNQGKXDEALEILQNSSSSTPHKTNDLKTFIYFVKDCVLGKTESQMVCKIDFAMENTLQLANVLSTCNSFQEMKIIYNEFLGNSEAREXKGXQNFVIVSLEAKLEILDQMLNNFESVLQLQPESPVVRESVSHKLSKYFAFKNFLQLPQIGATEMMKIMDIWSHVSPKNLDFVFNNIAETLYYDKYCDEIIIKGDCLLSADTETLQSVFGKLIEFFQASNQSEKVGKVETLQKLLEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZA6</accession>
    <name>A0A1E5RZA6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g16</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZA6"/>
    <dbReference type="FunCoup" id="A0A1E5RZA6">
        <property type="interactions" value="293"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZA6"/>
    <dbReference type="InParanoid" id="A0A1E5RZA6"/>
    <dbReference type="OrthoDB" id="2499604at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_transp_PIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31794">
        <property type="entry name" value="AUXIN EFFLUX TRANSPORTER FAMILY PROTEIN (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31794:SF2">
        <property type="entry name" value="AUXIN EFFLUX TRANSPORTER FAMILY PROTEIN (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="297"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="339"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="370"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="406"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ92287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="433" mass="47123" checksum="11B0943EB8F5FF55" modified="2017-01-18" version="1">MSEQGIAIRDHQGLSIPHLAYLTFESITEVVLVSVTGFICAYTGMLPKQGQKTISMLNVDIFTPALIFSKLAKSLSVAKFLEISIIPFFYALTTGISYIAGKTLATIFKLDIDETNFVIANSIFGNSNSLPVSLTLSLAYTLPGLTWDDIENDNRDNVASRGILYLLIFQQIGQVVRWSVGANKLLKWSGESQSSIDHGYDSAQSSTAPSSVGSLVPGAIENGDNDAGNNGLLYVDMENNTLLGKLWNSMKRAFAAFTHYMNPPLYAMLFSVIVASVHPVQHAFFHKNGFINNTVSAAITELGSLSIPLILIVLGSNLNPTNETAPKSYNYNKLVFCSIFGRMILPSLILLPTIALSVKYVKISILDDPIFLVVGFILTIAPPAIQLTQITQMNEFFEAEMAGVLFWGYCILTLPVTIAIVISAIGVLKWSGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZJ8</accession>
    <name>A0A1E5RZJ8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RZJ8"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="355"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="417"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="439"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="711"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="750" mass="83311" checksum="335BF126D8A0F073" modified="2017-01-18" version="1">MNTLTGQTNTYTNHNSGSRRKKNSLKHQQQVNSTLYQIPEYFEEPVKNDNRENSIESLIRGNTNYITTTNNTLTPCNSGNGLTPTNSANTLTPLNSNNTIISAGSGANHNNHNNNNNNLDNDSKLXVNNPSRNKYASKLLSNRNVSTASLYQNIMAKRNSNVTIRSGSNGHIIVSDNNGTASSNPMANGDTEGLGNTYNNGINHKDNRGSANSLNRGSNSSSNSTSSTNSIIYDRLQQGINPMTHLELKRINSIRPSSATILTKPNTITIENMKNGAVNNKKNGIFNEKFHSNNDINPRDLYFKRSKSEQTLKNKDFGSSREKLCIDEYTNGKKNSEFETDQHIFRSNFVLGGKTKSHSTESHLDKLGNLNKNTSSDGKFNNECDETEDKNEDHENISVSSSNQSPLNTTTLTSAVLSRNSSRRNSSNLSTNLFTNTSVPKENTQSSNNKTSLFQKQPITRAQLKMDAMKSKFDMFNSLDIPITSSIESISDELNKNKNTNAIKNKSMSASTRAKTLENNESTSTVPTSVLDYIGSASAKQHQPAFPSSLSRKVSFTSSNDSSLNDKAQLDKGFDSHLNDLQTELHTKNKKYWMVNNDLETRLLNEKINRQLDTIQRRSQTHGSKLLHKHVEHCYKTTIKNELYMQKLDQVISAQTSSIEVPKYLNPTIDTITSTDNIVSDEIQSNVVDAHDLLNFQQKLHSLKNGSSTGDGASDNYDLSAIKNSNLRKLITEGLGKIEDIWYTAESSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZS4</accession>
    <name>A0A1E5RZS4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab proteins geranylgeranyltransferase component A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g237</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Substrate-binding subunit (component A) of the Rab geranylgeranyltransferase (GGTase) complex. Binds unprenylated Rab proteins and presents the substrate peptide to the catalytic component B. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the Rab GDI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZS4"/>
    <dbReference type="FunCoup" id="A0A1E5RZS4">
        <property type="interactions" value="211"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZS4"/>
    <dbReference type="InParanoid" id="A0A1E5RZS4"/>
    <dbReference type="OrthoDB" id="1923006at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005092">
        <property type="term" value="F:GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.519.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018203">
        <property type="entry name" value="GDP_dissociation_inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017230">
        <property type="entry name" value="Mrs6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787:SF4">
        <property type="entry name" value="CHM, RAB ESCORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787">
        <property type="entry name" value="RAB GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00996">
        <property type="entry name" value="GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037514">
        <property type="entry name" value="Rab_ger_ger_transf_A_fun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00891">
        <property type="entry name" value="RABGDIREP"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00894">
        <property type="entry name" value="YEASTMRS6P"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR037514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ92467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="500" mass="56454" checksum="92D91E222CF0A774" modified="2017-01-18" version="1">MADQLDEVDVVIVGTGVAHSVLASALVWQGTSVLHIDCNEYYGDSTPTFSITQLKEWSDKTKNVASGSLYSNLDFEIKEDQFTEYQNYQHKHFSIDLHPKILFAESEMLALLIKSRVHQYTEFLPLSNYYTFCGETERFTKLKTTKNEIFIDENLPLLTKRNLMKFLKIVLNWEQESEKWEHYKDKALIEFLKEKFKLSDMQISEIVYTLGLSFEKNISTETAIERIKKCLASYEVYGPFPTLFSKYGGVGELAQGFCRSAAVGGCTYKLKTRITSYDASCKEITFQDGTKTKFNKKIVYSPMQESCLQNETGLFKGSLFHRLIAVIKKDLSKEWYKAGESAAVLVFPPGVLGETDKKNTNSVQVVLYGSSSELCAKGTSLVYISSIDSQDCLRAALRQFLQSSGASKEDVVLKQTYSQKSLLPFSIPTDYDFESVIESSDQNHVIVTPPPSLEVSYEDGSIGVAKSVYKKLLGQCDDFFAIDLEEEAAEDEESRRYSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S056</accession>
    <name>A0A1E5S056_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA polymerase II-associated protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g51</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PAF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5S056">
        <property type="interactions" value="337"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S056"/>
    <dbReference type="InParanoid" id="A0A1E5S056"/>
    <dbReference type="OrthoDB" id="10260285at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007133">
        <property type="entry name" value="RNA_pol_II-assoc_Paf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23188">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23188:SF12">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03985">
        <property type="entry name" value="Paf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="128"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="5">
        <location>
            <position position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ92439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="397" mass="46161" checksum="32C64D81B9B62750" modified="2017-01-18" version="1">MDRKYIARVKYXNDLPPPKLPPKLLKYNADEQEFLKNEVNNPSLITTLSTKNDISQLVKINEDLGMELNLMKFPGVLDNNDDKMLYGFENIKLAPEDRVLLRNPGVDRLTKTDISKVGFLRRTEYTSSTLTSRPQSASANPFLNNPKHGRKRKLLDSNGNYTDQYGAENVDAEELEDLVRLDSKKLVTKIESTFQTLDANMDKYKHPVKKNLKAKKVWSFLPDTVSMDEKFFMVKLVGSAVLDKAEREKLSLDSALFRPVELNEDDWMSFYSLTDKSKSKKLINEMEHVLQDKDTLNASDTFKFQRVRDYNMHHIQFHDQHDLAIAFNSEKNTAFYKPIQSRIELKKRRVNDFLKPLVKEHTVDQINVVFNNPTTEEQMFKDKVRSEFDPIDFPDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S093</accession>
    <name>A0A1E5S093_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g104</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S093"/>
    <dbReference type="InParanoid" id="A0A1E5S093"/>
    <dbReference type="OrthoDB" id="4034134at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038872">
        <property type="entry name" value="Put_GTT3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41807">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41807:SF1">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="355"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="3">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ92479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="380" mass="43547" checksum="514DC481BA9C27AB" modified="2017-01-18" version="1">MDTSYIMDTLTKYTKRDLMTMVKALPVDAKITTKTVKPKLIEIISEYFGSASSEEIESLLSKRHQLLREVIDSPGKKLEVNGSNESEEEESEDEESVEEENVEEEEEKEEPPLKLEQDVIDEQVLDESPEESXEESPEESPESSAGESTEESTEDSLKEETPVKTLDAEAVEEXVIEEVAEKTAEDIKETKEFFNHLNFNNITSNTPFGFKFPYYDEILDQNEQLRQFLSQYTVVQYLCCLIELCVLVYTPVCELALGMRGDINANININNSITNSNFTLLFYGMLKLTKLMVYHLIEHYALPSVVGYYVNFIRQDIDECNIDPFIFHSVKIFVSIFAYKCELWKGGQTCEYNNMLINSGCFTVSILSSVFNIFLILYSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S0A3</accession>
    <name>A0A1E5S0A3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Flocculation protein FLO1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g76</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S0A3"/>
    <dbReference type="InParanoid" id="A0A1E5S0A3"/>
    <dbReference type="OrthoDB" id="3973523at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000128">
        <property type="term" value="P:flocculation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001389">
        <property type="entry name" value="Flocculin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018871">
        <property type="entry name" value="GLEYA_adhesin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00624">
        <property type="entry name" value="Flocculin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10528">
        <property type="entry name" value="GLEYA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184968" evidence="1">
        <location>
            <begin position="26"/>
            <end position="1468"/>
        </location>
    </feature>
    <feature type="domain" description="PA14" evidence="2">
        <location>
            <begin position="90"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ92489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1468" mass="151048" checksum="6B61D9E5D96EF827" modified="2017-01-18" version="1" fragment="single">MIPNSIKTLLVFSVFFANFFNFTNAVGSTSQGDVVGCNPDNVALQGLNMTFYYYPWLKKSASGTSNTADLTTYKTDAYINGGYADYPILATASDVTNITYATTSTQIPNSRTEVWGQLPSTMKATPNNVSLSNFAFLATGYIGIPDDYFGSDDIRESYSITFTLNYIDDYSVVSIGDGIAYNCCASDTAVTQNGYQFQSLWTGGQPSGINTVTMTLYAGYYYPIRIFYVNRNYIGAITMTYSVEGETATDDMSRIMYMAGDQEGGCSVPSLSTTISDYLYITAPTTVAGISTWTSTVSGVATTETGFAYVVEYPSLTTGTDVSYTSYYTGTTTSKFTTTTWYLNTDGVYELQTVVSEADPYSSLNTTTYKPWTGSVTTVYATSTQTVTNNGVPTVETVYQVLTPLESLTTTTSYTPYDGPTTTTVGTTTTYLTTDGFTYTTVIVEVDTPAAFGTTTSFTAGSVASVTTVGTSMTTIAGSDGTDTVSTYYTVEVPIVSTTTTSFVEEVVEKTTTLATLVTTIVPTDGSPATVETVYKVAVPQKTAQFTVTNVADVPSTTTIATRYTTVQGSDGIDTTSTIYSVDIPGAFTTSTSVVTGAVNATTTVGTAVATITGADGKATISTLYTVDVPVASVVSTTTTYGDVASATTVGTAYTTVVGSDGASTVSTLYTVEEPATSVVSTXVVTGAVNATTTVGTAVATITGADGKATISTLYTVDVPVASVVSTTTTYGDVASATTVGTAYTTVVGSDGASTVSTLYTVEEPATSVVSTXVVTGAVNATTTVGTAVATITGADGKATTSTFATTTVGTFVTTATGADGKATTSTLYTVEVPSANLVATTTTYGDVASATTVGTAYSTFTGSDGSSSVSTIYTVEFPGTGVVTSSVVTGDVSATTTVGTSVTTITGADGKATASTMYTVVVPSAEFVATVPTYGDVSAVTTVGTVYSTFTGSDGSSSVSTIYTVEFPGTGVVTSSVVTGDVSATTTVGTSVTTITGADGKATASTMYTVVVPSASVATVTTDVYTAYNGTTPTTYSTSTVVATGSDGKETSSVVYYVSTPVGNAYSTVFTPGTVTAPYTYATNVFTTVGTDGLSTTSTIYYVQTPIIFVTTDVLTTSDVASMVTYSTGRVTTVDTDGAETISTIYYVATPVGSSSLPSAVSTAFSEYDGTVTSTYSTSVFTTTDAAGKAVVSTIYYVEYPRSGAASTYYTTYGGSAVSTYGSDVFTTTNAAGTPVVSTVYSVELPVVSSSSTTYTTYDGSVTSTYSSPVLTTTDADGFVVVSINIIIQVPASPSVSTYYTTYGGSVTSTYGTGYMTTTDAEGHTVVSTVYSVEVPASPSVSTYYTTYGGSVTSTYGTGYMTTTDAXGXXVVSTVYSVEVPASPSVSTYYTTYGAAEPRACGTCRSPRPPAQDHTDVSTVYSVEVPRSPSVSTYYTTYGGSVTSTYGTGVFTTTDAEGHTVVSTVYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R021</accession>
    <name>A0A1E5R021_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative metabolite transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4644</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000017">
        <property type="protein sequence ID" value="OEJ80237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R021">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R021"/>
    <dbReference type="InParanoid" id="A0A1E5R021"/>
    <dbReference type="OrthoDB" id="2261376at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046943">
        <property type="term" value="F:carboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="316"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="417"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="456"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="495"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="96"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="51"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="576" mass="63436" checksum="68851A403471FF2D" modified="2017-01-18" version="1">MSDIDQRYINHPYTRDLPFSFRELCTNWYTRSSKEIVTCLPFMTPQRKVVSDARKTELETSGESESNSLADDSEDFEDNSEFGSLTKEEKNTAIWNMFIAAGNLFSDGYANSNTSVITLGLSTIYGKEYTNSTAIGNVSSVVFAGTILGMIFGGLWADNLGRKSGLLVSNAFLIVFIILCAGAWGAHTSLNPPKEGGLFGAIIAYRFLLGIGIGAEYPLGSAMSADVSQHMQKSQRSKWFCWFTNSAISFGFVMSSFIAYIVAVICGKNHINTIWRVAVGIGAIFPMIFFVLRLFMKESDTFKKNRFTKXVPYVLIYRYYWFRILVVSISWFIYDISSYGFSSFSSIILKNVEGGSPSVIQTLGWNCLFQTFNFPGAVIGAFFTAAYGPRLSGGVGVIVQSIIGFAMAGAFNTLRKHIAGFTVMFGIFSTMGNFALGNQMGLLATLSFPPECRASAYFICSVIGKVGAFASGWIFPKVIISAGGYSTSSGLAAPYWLACSLGCFAGFLILVFLHPMDAKSQDEEQMKFRFYLEQQGFDLTTLGVRVEESEVESVHTEKESFEGKENTVVLTRVSTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0C4</accession>
    <name>A0A1E5R0C4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4405</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0C4"/>
    <dbReference type="InParanoid" id="A0A1E5R0C4"/>
    <dbReference type="OrthoDB" id="3026777at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507:SF1">
        <property type="entry name" value="FI18259P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507">
        <property type="entry name" value="ZGC:174356"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="373"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="412"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="453"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="479"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="513"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="541"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="581"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="581"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="610" mass="68327" checksum="B1B39DAF39C7D1E6" modified="2017-01-18" version="1">MSVSQNKSTSKSSPYNDPLVAAENENFILESTFEELLSKNSVLGNISRDTANQATYSVDGELIKDSNHGDRSYECASFFDFKHFKFNLKPNFYLLVCLSALKFVASFICVTALSDLKMRKVVGSFEDSNFGQSEYLRVQSFVSAFQSVTGIITSSYFGSLSDRKGRFFAFVLSAVLSVMAGLINLFIFSDRYQYNIYSFLLLLSVSTLDGGVVVLNALTNSYISDIVPSNERLLFFSTYQCVICGLSTVVPLLTAFIVNKTSNYTTLLFSMVLYVLTLIGTWLFLSESLSTEARQESMTENQKTIEKYKMLREKCFEDIRSSDSSSRGNLFSKVFRAIYHTCRLHVVVDVVRPLKALYLPKTSTGSLIPRVNALILVASQVFYLASYDGAGVALTPYALIDLQWTSVDLSYFFSYSSVISTITLLLVPIYLYPYLIQKRGFKILTFSVDKLDILNMRVLSVGLMFSVVCPLLFISTQGLYGCTFFFGLVSFAVPTIQNSLLKYAAPNKTGEMLAGVSLLSHLNVLITPPIFLQVYDKTKSYWPTLFLVIPLTFQTCGFVLTYFLKIVEDPERFDVIKDNTSSFTPQNPLHDSSSETDSLLSSSSERRESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0N7</accession>
    <name>A0A1E5R0N7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 13">Deoxyuridine 5'-triphosphate nucleotidohydrolase</fullName>
            <shortName evidence="13">dUTPase</shortName>
            <ecNumber evidence="5 13">3.6.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 13">dUTP pyrophosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g4537</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in nucleotide metabolism via production of dUMP, the immediate precursor of thymidine nucleotides, and decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>dUTP + H2O = dUMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10248"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61555"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="3.6.1.23"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:10249"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the dUTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.23" evidence="5 13"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0N7"/>
    <dbReference type="FunCoup" id="A0A1E5R0N7">
        <property type="interactions" value="1154"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0N7"/>
    <dbReference type="InParanoid" id="A0A1E5R0N7"/>
    <dbReference type="OrthoDB" id="419889at2759"/>
    <dbReference type="UniPathway" id="UPA00610">
        <property type="reaction ID" value="UER00666"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004170">
        <property type="term" value="F:dUTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046081">
        <property type="term" value="P:dUTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.40.10:FF:000007">
        <property type="entry name" value="dUTP pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008181">
        <property type="entry name" value="dUTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00576">
        <property type="entry name" value="dut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001862">
        <property type="entry name" value="PRK00601.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11241">
        <property type="entry name" value="DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11241:SF0">
        <property type="entry name" value="DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="dUTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="dUTPase-like" evidence="15">
        <location>
            <begin position="27"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ80464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="157" mass="16643" checksum="FDFE1037A80E3F10" modified="2017-01-18" version="1">MSIDKLETTNKQQKLSSQLKVQLRSKDATAPTKGSIYAAGYDIYASKPTTIPARGQGMVSTDVSFTVPPGTYGRIAPRSGLAAKHGINTGAGVVDRDYTGEVKVILFNHSEKDFEVVKGDRVAQLILERIVEDAAVVVVESLEESARGAGGFGSTGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R165</accession>
    <name>A0A1E5R165_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="6">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g4271</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PPP phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R165"/>
    <dbReference type="FunCoup" id="A0A1E5R165">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R165"/>
    <dbReference type="InParanoid" id="A0A1E5R165"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07415">
        <property type="entry name" value="MPP_PP2A_PP4_PP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="8">
        <location>
            <begin position="112"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="209"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="357"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="209"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="219"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="358"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="402"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="412"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="473" mass="54173" checksum="BE6D869BAE64E5C7" modified="2017-01-18" version="1">MSFSSYELDQCLERLYKGELLPEVVIEAVCYKLKEILVKESNVVQLNTPVTVVGDIHGQFHDLLEIFKIGGKIPDTNYLFLGDYVDRGLYSVETITLLIVLKLRYPQRIHLIRGNHESRQITQSYGFYTECLNKYGGDSQVWKHFTDLFDYLVLSAVIDNELFCVHGGLSPNIQTIEQIKIIDRIREIPHDGAMADLVWSDPDETFSGGDSNTSLIGDNNSNSNSNSNSNNGNTNGISHNPNNPNTSSLMDEHFQVSTRGAGYLFGGNVVKKFCHLNNISRIYRAHQLCNEGYQVYFDGLLTTVWSAPNYCYRCGNKASILELYSVNDYHFNVFNESPEYNVMNGNRKLLNPSFFSAFDDEDEDDEDEDEDEEEEEEEEEAEEEELYGVDEQESLDMIKGLQRNSSKNGTANRDDKMSKKVERGRKKLKQMQQVQELQKAGLISGDFMNDRVDIFSDSYQRENSQTRRVEYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R253</accession>
    <name>A0A1E5R253_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Golgi pH regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4111</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R253"/>
    <dbReference type="FunCoup" id="A0A1E5R253">
        <property type="interactions" value="157"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R253"/>
    <dbReference type="InParanoid" id="A0A1E5R253"/>
    <dbReference type="OrthoDB" id="3972504at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025969">
        <property type="entry name" value="ABA_GPCR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022535">
        <property type="entry name" value="Golgi_pH-regulator_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015672">
        <property type="entry name" value="GPHR/GTG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15948">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 89-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15948:SF0">
        <property type="entry name" value="GOLGI PH REGULATOR A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12430">
        <property type="entry name" value="ABA_GPCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12537">
        <property type="entry name" value="GPHR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="117"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="192"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="311"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="391"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="458"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="513"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="Golgi pH regulator conserved" evidence="7">
        <location>
            <begin position="182"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Abscisic acid G-protein coupled receptor-like" evidence="6">
        <location>
            <begin position="309"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="548" mass="61806" checksum="1E79BCCA2C49FE5B" modified="2017-01-18" version="1">MEDFAIFILLGISHLAAYKVAHDHVWFQINNLFDILPATAATAATPQDQQFFQVIQDTSIEDRFLYRFYSKYSFSSKYVHKCVRALFSLVLSLSVVVVEIVLWKIKTYDDVEKTDSVIAVVWPVVIASLSFSLILLVPYLSALMIFNKFYTDRLSTDALLVSTAAFILVWLLGLRAVSIGPFFYTESLLTKLALLGITIMAILSGIASISTPHHVLLSVWKRRQGFYDNSASIFPRASKGFSSTVDINSEMIKEYEKNIEENLAILKKFKELPSNGSDVFLKQQLIEKIAWYKIELDKLKLVTGKPRSVKILYNAFQLTFFLFCIFKLIVVFGINLPKQISYRILSPKDIHFENFGKENEADPLAVSLANILDIVLFTFDHQQELDSLSKLISLIFSLSMFACSLSTVLSTISRLLSILPLKFQLFALSVIALDQKNSKLPVSTKDNQTSMNEAEPSIIKNLIFVELVGVYILATILLIRSNLPYDVSEKLNSFLGSKFTVPSVNIDIWFDEIFALSAVLTLIGFVVTSIKPFELSSAPGSFKRRTSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2A2</accession>
    <name>A0A1E5R2A2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">proteasome endopeptidase complex</fullName>
            <ecNumber evidence="3">3.4.25.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4043</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.25.1" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2A2"/>
    <dbReference type="FunCoup" id="A0A1E5R2A2">
        <property type="interactions" value="1002"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2A2"/>
    <dbReference type="InParanoid" id="A0A1E5R2A2"/>
    <dbReference type="OrthoDB" id="7854943at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03762">
        <property type="entry name" value="proteasome_beta_type_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000010">
        <property type="entry name" value="Proteasome subunit beta type-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF0">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE SUBUNIT HSLV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="8 13" id="KW-0647">Proteasome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0888">Threonine protease</keyword>
    <keyword evidence="9" id="KW-0865">Zymogen</keyword>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ81036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="215" mass="23588" checksum="D6F2A99245513635" modified="2017-01-18" version="1">MDGIQVDIQHLKKGEVSLGTSIMAVTFRDGVILGADSRTSTGAYIANRVTDKLTRVQDNIWCCRSGSAADTQAVADIVQYHLDLYKAQFGEPSTKTAASIFQTLCYENKDNLSAGIIVAGFDKKNKGEVYSIPLGGSLHKQKYAIAGSGSTFIYGYCDKNFKEDMSKEETVNFIKHSLSQAIKWDGSSGGVIRMVILTEAGVERLIFYPEEYENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2C5</accession>
    <name>A0A1E5R2C5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">2-dehydropantoate 2-reductase</fullName>
            <ecNumber evidence="2">1.1.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Ketopantoate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4014</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ketopantoate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.169" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2C5"/>
    <dbReference type="FunCoup" id="A0A1E5R2C5">
        <property type="interactions" value="121"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2C5"/>
    <dbReference type="InParanoid" id="A0A1E5R2C5"/>
    <dbReference type="OrthoDB" id="73846at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008677">
        <property type="term" value="F:2-dehydropantoate 2-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003710">
        <property type="entry name" value="ApbA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050838">
        <property type="entry name" value="Ketopantoate_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013332">
        <property type="entry name" value="KPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00745">
        <property type="entry name" value="apbA_panE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43765:SF2">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43765">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02558">
        <property type="entry name" value="ApbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketopantoate reductase N-terminal" evidence="6">
        <location>
            <begin position="4"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Ketopantoate reductase C-terminal" evidence="7">
        <location>
            <begin position="230"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ81034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="392" mass="44430" checksum="D710825A948A80BD" modified="2017-01-18" version="1">MTKFHVLGLGSIGAIIANSLQELQKTTSSLQIEVIPIIRNKSKVVDFGKNHDFKLKINQLYHKKTVISDPFELTTCPEMLNESKDTLTREPIENLVITTKTHQTVAALKPIWNERLINENTNIILIQNGFGMLEELITAFPEIASKNFKVFQGVISHGVFMENYPNEFNHAGFLDLKIAMINGMEDNGIKGLQTVQDLQKLKQENALISVFQHLNLDVKVLTFQELTVGQIEKFCVNCCINSITTILNCVNGQLLVNEVETRKLFTNVITEVTTLFAQSEKYKNIFNYYNVIENVANKENYPNTIDLTKLNNIPKLLDYVVDIGCVKNRENSSSMRQDAINKRDTEIDFINGYVVRLCLQELSLPVSHCSVNNTIVSFVKVKNQLLKETSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2E8</accession>
    <name>A0A1E5R2E8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Vacuolar protein-sorting-associated protein 25</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g4195</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2E8">
        <property type="interactions" value="812"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2E8"/>
    <dbReference type="InParanoid" id="A0A1E5R2E8"/>
    <dbReference type="OrthoDB" id="3971336at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000814">
        <property type="term" value="C:ESCRT II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.570">
        <property type="entry name" value="Winged helix' DNA-binding domain. Chain C. Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008570">
        <property type="entry name" value="ESCRT-II_cplx_Vps25-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014041">
        <property type="entry name" value="ESCRT-II_cplx_Vps25-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13149">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13149:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN-SORTING-ASSOCIATED PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05871">
        <property type="entry name" value="ESCRT-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ81060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="204" mass="24199" checksum="234DDCD19E006F55" modified="2017-01-18" version="1">MSFTELPKIYDFPPLYTKQPNXLVFNKQLETWSNIILQYCQANKIFRMNDQRLRMDLFDNKKINRSVSLDLEKKIWDHVFQAQSRIVYLKEDPSTSASSCRKIQIDKNPIKKAEYGIVLWKSIEDWGSIVLNYMVNSANGSNSIFTLYELENQSYNELFHNIDHELLKYVVQEELCKNRKRVVLMYEEDPEEDQYGELVGIKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2U3</accession>
    <name>A0A1E5R2U3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nucleoporin NSP1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Nuclear pore protein NSP1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Nucleoskeletal-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g3897</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Nucleoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the nucleoporin NSP1/NUP62 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2U3"/>
    <dbReference type="STRING" id="56408.A0A1E5R2U3"/>
    <dbReference type="InParanoid" id="A0A1E5R2U3"/>
    <dbReference type="OrthoDB" id="344345at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044613">
        <property type="term" value="C:nuclear pore central transport channel"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000040">
        <property type="entry name" value="Nuclear pore glycoprotein p62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026010">
        <property type="entry name" value="NSP1/NUP62"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007758">
        <property type="entry name" value="Nucleoporin_NSP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12084:SF0">
        <property type="entry name" value="NUCLEAR PORE GLYCOPROTEIN P62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12084">
        <property type="entry name" value="NUCLEAR PORE GLYCOPROTEIN P62-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05064">
        <property type="entry name" value="Nsp1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin NSP1-like C-terminal" evidence="16">
        <location>
            <begin position="406"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="84"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="150"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="344"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="503"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="532"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="97"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="156"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="287"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="508"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00078941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF05064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ80893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="607" mass="63578" checksum="A1CE573FF94952D1" modified="2017-01-18" version="1">MSTPFAANSAADPSSTIHQGLSKPEDQNNNQQASVSSNASKQFSFGQNTAQGFSGFGSANAAAKPNIFAQNQIVSEGKKLFGATATGAPKESSAPASLNLGSSSGTSSLSAFAKPAGTAAEPAKSGFTFNLSNEKKGAFEKPIFDLNSRSFKPAAQVSSTSTTPANTVTDKKDEPAKSGFAFGGSEKTAEPAKPAFAFGGSEKTAEPAKPAFAFGGSEKTAEPAKPAFAFGGSEKTAEPAKPAFAFGGSEKTAEPAKPAFAFGGSEKTAEPAKPAFAFGGSEKTAEPAKPAKPAFAFGGPEKTAEPAKPAFAFGGSEKTAEPAKPAFAFGGSEKTAEPAKPAFAFDGSEKKKETLTPAKQSGFQFGAKTAEKVEENNKPAGNNSGAPPAKTETVTTTNPASVVQLPTQAPEINLDNKTLDDLVSKWARSLDTSYSHFDAYLGKVNEWDRVLVDGGEKISKLYSDALMAEQTQNRIEQSLQYIERQQTDLEVFLNNFEQKTEKLLSDISPSAPSSSNTTGSNDEKRKQAHQTALQLDDNLNSLSANLASLTEEINEVSENFTKNSQDSYNNKEEGVQIIKLLNSHLDALNALSTSSDSLEQKLKTLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R3H5</accession>
    <name>A0A1E5R3H5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein STU2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3857</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R3H5">
        <property type="interactions" value="93"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R3H5"/>
    <dbReference type="InParanoid" id="A0A1E5R3H5"/>
    <dbReference type="OrthoDB" id="205662at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061863">
        <property type="term" value="F:microtubule plus end polymerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030951">
        <property type="term" value="P:establishment or maintenance of microtubule cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046785">
        <property type="term" value="P:microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007051">
        <property type="term" value="P:spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048492">
        <property type="entry name" value="Stu2_CTS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045110">
        <property type="entry name" value="XMAP215"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048491">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12609">
        <property type="entry name" value="MICROTUBULE ASSOCIATED PROTEIN XMAP215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21042">
        <property type="entry name" value="Stu2_CTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21041">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TOG" evidence="6">
        <location>
            <begin position="3"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="6">
        <location>
            <begin position="308"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="565"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="729"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="817"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="697"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="580"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="593"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="609"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="729"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="7">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ81123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="890" mass="100339" checksum="4A45AF6BA92D073D" modified="2017-01-18" version="1">MDDYTELPLKERLTHKLWKARAHGYEEVQKMIASVKNKPYTKLLELHHEYNLVSFVEDTNVVALEKATCTVYDFLSLLQRQLLQGATPRTYENIFKKEVAPIRDSLMRVVLLKCCNSSRRLTKEKSLKIVTIMVQLDVNINFFIETVVSLIFNNLKNVKNLNGFVTTTSFILAEFQVMPTNIHLVEPFLKTILKLSTNADVKIRQETFEIIAILDVCCDANKDVLHDLVLCNWKSSQLSEFNQKVQILVDGGKNREKVHFEIDLIREQHMQASKEPATVTQTNHSIDSDGDTIMNGFDSNVKXIDPFEMYTPTSILPDFDLREFSSMLHSAKWKDRVETLQHLNDNVLKNVKKLNYQKEDYTPIFQLLAENIAKDSNLQAVQLSVQAVNKMLNGLKYNAVHKYSHLLFVPMVERLKDKKVNGLVFDTLATLVDYNNEGLKFCLDELILRFLQHKILQVRAQSCRLLTELMLQNATDVPKLISIDRLTNDVLPLILKICNDSQLAIRKIGFENIAAFIILMKNGQEELMPVLEAKLDQLKLKEITKLTTELVVPVKKQKILKNQSNTISSTDQKPKIAASGISSQPESTLPSKRLAHSPLKKPHRESSHIELSSNNHYSHPLNKSSPTKNSPSLTNFSTNSKTSRKSTFSESQETDPAWSVEKKILLSRISGLETENKYLVSEMEQYKLKITTKATALDDYKLKCSTLEEKLRLANAKIKSLQSVIDSGVAQNSPKMEQNGSKPEQDAGSSSSYSNMVSIPLSSNRSTTTSSEDYRRAKRYNSSSSFVYVDESGNHSSITKLPKASLSNEDLNKGVDSLKLSSGNRPEAVKGSSPALLSDSFVPSSSSAALQSAITEESWSKAAEVTRQLKARIERMRAKTRSLSSQGGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4Q2</accession>
    <name>A0A1E5R4Q2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3579</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4Q2">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4Q2"/>
    <dbReference type="InParanoid" id="A0A1E5R4Q2"/>
    <dbReference type="OrthoDB" id="3973107at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030695">
        <property type="term" value="F:GTPase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08368">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09397">
        <property type="entry name" value="LIM1_UF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24205">
        <property type="entry name" value="FOUR AND A HALF LIM DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24205:SF16">
        <property type="entry name" value="GH01042P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="7">
        <location>
            <begin position="581"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="20"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="197"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="242"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="318"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="520"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="723"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="61"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="113"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="254"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="268"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="278"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="729"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="8">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ81872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="764" mass="85016" checksum="CFC8F389C125FA1C" modified="2017-01-18" version="1">MYSSPFPPLNPRVKYRTAMERAGFDVNKSEEPSADNSPNATSNNTPNASKTDLFELNNNGRSRAVSSSTTASTGLGKLMNKLKNNKGTTTNNGSKSYSSSNSPSPLSAKFKHYNNSTNTNPDTSFRSDIDVSRNNSTKNKGYGFNDLTSSIVKGDTSFINENEAQNDTSTXKNDTNKSITDLSKSGEFNTDLLEASKSNTDLSESGKTNVGANTDNDGVQKFNPNTTLEHARQKSFDMLTASLGGDTHEKDTDKDDNDEDDDDDDADNVDKVSHGRQLDTSSQLDTNSPSKAISHNIPSVVMNNSVLDQVAPLSFSSPTKSLKRVSAKDPNMEEQEQETRPENTEIANLKKDQQQNLDFNHIQXEQPCNTQTSQQLHDLLSELNKASESKALLANSIEPRSKGSAAFGASTSKLSPHLTDNVSSASTVVNDNGYINNNINTTKSTTQDNIQRALSEHMSESSGSHYSEYSANGELPVDSQNGIKFNSKSSAYLSGFPLTWGKETEMLAKALQQDEMDTKTPIDHDFDVKTGNERGTGKEFQNDTVEDVLQKDVIPDFPQKSSLSKDVFEETTEIKYPPGQGPCRKCHHEIDPVKEKAIYSKSGQDKTQDIDVLSGQWHRRCFQCTLCDLKFNKRTPCYVLHDLPYCQYHYHLTNNSICTICSGFIEGECLENDQNERYHVHCLTCYICHEIINKDYYIYNNKLTICENHDLDDLLKRGIPLEGEEKAEDSDKNPESGDDVVDRKDKSKKTENYVSKRRTRIIEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R519</accession>
    <name>A0A1E5R519_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycogen debranching enzyme</fullName>
            <ecNumber evidence="5">2.4.1.25</ecNumber>
            <ecNumber evidence="6">3.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Glycogen debrancher</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">AWRI3579_g3504</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Hydrolysis of (1-&gt;6)-alpha-D-glucosidic branch linkages in glycogen phosphorylase limit dextrin.</text>
            <dbReference type="EC" id="3.2.1.33"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1-&gt;4)-alpha-D-glucan.</text>
            <dbReference type="EC" id="2.4.1.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the glycogen debranching enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.25" evidence="5"/>
    <dbReference type="EC" id="3.2.1.33" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R519">
        <property type="interactions" value="416"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R519"/>
    <dbReference type="InParanoid" id="A0A1E5R519"/>
    <dbReference type="OrthoDB" id="10248904at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004134">
        <property type="term" value="F:4-alpha-glucanotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004135">
        <property type="term" value="F:amylo-alpha-1,6-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005980">
        <property type="term" value="P:glycogen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000039">
        <property type="entry name" value="Glycogen debranching enzyme Gdb1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000242">
        <property type="entry name" value="Glycogen debranching enzyme Gdb1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010401">
        <property type="entry name" value="AGL/Gdb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032788">
        <property type="entry name" value="AGL_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029436">
        <property type="entry name" value="AGL_euk_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032792">
        <property type="entry name" value="AGL_glucanoTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032790">
        <property type="entry name" value="GDE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10569">
        <property type="entry name" value="GLYCOGEN DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10569:SF2">
        <property type="entry name" value="GLYCOGEN DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06202">
        <property type="entry name" value="GDE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14701">
        <property type="entry name" value="hDGE_amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14702">
        <property type="entry name" value="hGDE_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14699">
        <property type="entry name" value="hGDE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="14" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Eukaryotic glycogen debranching enzyme N-terminal" evidence="18">
        <location>
            <begin position="49"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Glycogen debranching enzyme glucanotransferase" evidence="19">
        <location>
            <begin position="166"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="domain" description="Glycogen debranching enzyme central" evidence="20">
        <location>
            <begin position="773"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="domain" description="Glycogen debranching enzyme C-terminal" evidence="17">
        <location>
            <begin position="1111"/>
            <end position="1565"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="21">
        <location>
            <position position="848"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF06202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF14699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF14701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF14702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OEJ81994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1574" mass="179300" checksum="EDBF3A63FC51E850" modified="2017-01-18" version="1">MPVKVLLKLNDDGSPIVRKAMGNARGVIVLPPTDESGASEDPGCEYELNLFIQAGSSLTNNGTLVLNTTFAGKIGQYDISNDFETTKSIDYQKNKNIESIESIKSKQKAHTDKNVKICLNKPGPYYYYITYTPLEPTEKEDTKKSTEKCKTHDFYFIVPPKLPFGIKLDSVSLQTLVSKWIPICGKNENEKSNIETHWDPILNKIASKGYNMIHFTPLQERGSSNSPYSIYDQLNYDPQHFPHGLKDVKLLMNHIHKTHNLLSLTDIVLNHTANNSYWLKDHPDAGYNQCTAPHLKPAIELEQELTKFSDLISGNQLENISXNIETENDLINIMSSMESIVFQKNLKLWEYYIIDINKLTTKIKCLLNNSTDSEDGDGNNTGDIEEKKFISENYVSTLVEKCCLECQIDNDDQSNENDNKIIDIATERFGHQLDPLKFIDFLKTHFANNIGNANDSSINILQFATKILEQINSKQYTFYDNDIKTIKDQLYNRIKYLRLDSHGPKLGXITKSNPLIEKYFTVIEHTDGLQGKFYLANNGWIWNGNPLLDFASNKSKAYLRREVIVWEDCVKLRYGDSPKDSPYLWERMSQYIVENASIFNGFRIDNCHSTPLHIGTYFLALARSVNPNLYVLAELFSGDEQLDCVFVEKLGINSLLRESMQANSVQELSTLIHRYAGNPIGTFKNLNFDDYSLKHYDHQMLRSTQPPVLIMDCTHDNQTPYQKRTVEDTIANAALVSFSCSAIGSVYGFDEIFPYLLDVVNEKRNYSVVXNDGISQVKKKLYSIRASIAKDINDYEMFIHQDGDFVTFHRVNIKTGKGWYLIAKTKFTNSDDGNTEEKVXFNLASTFDCSLKFAYSLIRTGDPXDDPETIKGIPTKILEHDINDFKISISDDNNCDIAVPNSTFPKGSIAVFETEQHPAGWFLNKEVVEECVKASXSLTLDALNVLLYRCEPEERDFSAGAQGTYDIPNHGKLSYAGLQGWVSILKNITFNNEVDHPYCDNLRTGTWALDYMESRLDPYCEKFPEIVAFQKWLKEILAKVRLLPAGLRPAYFAVIVSLAYKCCVKKALQLMDLDDNGKEDDSTQVTLFQKQLGLVSVQMCSLNYSASLVPNTPVSSMAAGLPHFSSEWARCWGRDVFISLRGLLLSTKRFEEAKLHILGFAKTLKHGLIPNLLDSGNNPRYNSRDSVWFFVQAIKDYVTVVPGGEAILKERVKRRFPLNDEYLPLNDPKVYSYETSIGDIIFEIFQRHAKGIHFREANAGPQLDRVMKSEGFNIDIKVDWNTGLIFGGSQDNCGTWMDKMGESEKAHSVGVPGTPRDGASVEIIGLLKSSLNFVNSLHSKKLFEYDSVSREDGTSVTFKEWETLILNSFEHAFYVPLDPNDDFKYDIDSXIVNRRGIYKDLYKSGKPYEDYQLRPNVMVAMCVAPELFQFDHALNCIDKVDKYLKGPLGMRTLDPSDYNYRPYYINSEDSDDFATSKGRNYHQGPEWVWCFGXFIRAYYYFNKKRNQXNKDRKQDLELDVLNKLLPHKKWISESVWAGLTELTNKDGSLCNDSSPTQAWSAACLLDFYLDLXRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R587</accession>
    <name>A0A1E5R587_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphoglycerate mutase</fullName>
            <ecNumber evidence="9">5.4.2.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3704</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.11" evidence="9"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R587">
        <property type="interactions" value="573"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R587"/>
    <dbReference type="InParanoid" id="A0A1E5R587"/>
    <dbReference type="OrthoDB" id="354304at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00186"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004619">
        <property type="term" value="F:phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07040">
        <property type="entry name" value="HP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01039">
        <property type="entry name" value="PGAM_GpmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005952">
        <property type="entry name" value="Phosphogly_mut1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01258">
        <property type="entry name" value="pgm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11931">
        <property type="entry name" value="PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="6">
        <location>
            <position position="9"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="6">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="8"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="159"/>
            <end position="162"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="186"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="8">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ82061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="365" mass="41818" checksum="98C012EFC5C67D53" modified="2017-01-18" version="1">MIKLVILRHGQSSLNHENIFCGWIDAALTEKGLQQASEASNLIYDHFVTLKENGDAEHASKTKDESQLPTVGFASRLVRTNQTIAQIIKTLEQRSSPDNHHFDGVDEYHIVECKKNADINTAVQDKGQIVYKPVQQDGKDDNSGEYKGFKVYESWRLNERHYGSWQGQRKPQVLQKYGKDEYMRIRRDYNGKPPSVDLNKEMEVVPAALQQEMDKENRGSAVPKEXQSKPMVPTGSELVYDFKEPNRSLKYKDEYTYGNFLDLPTAESLNDVIHRLQSYLYDFMIPKILATDNKTGLIVAHGSSVRSLLKILCNISEEDIKNVDIPNGIPLVVELKEDFTFMKRYYLDPEQAKINAEKVRNEGFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R595</accession>
    <name>A0A1E5R595_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-alpha-acetyltransferase 40</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3592</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R595"/>
    <dbReference type="OrthoDB" id="424551at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="unsure residue" description="I or L" evidence="1">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ82085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="32791" checksum="717EB677804BB1AB" modified="2017-01-18" version="1">MVDFASDSILALLPAIFPTENKVKLKGEFDAIDKIQKLTREIKTGLTLSTHELDQHLHLLKENLGSYYDRHGSYMYEYDEDDKVNISKPNSDARENKSKWDEFKLEEMRLSELIYVSYYHCTENTKELAYYVSFLITDEDMRDTFNTGAGAHKLQSRKLRSKQLEVCNRILYVYELQLDQRYRRMGIGYXILNTLLPQVLNAHVQNEQVERDXDALGKLGNCAXIDKXXVEYIXAVQLCCFEDNHPAIGFYEEKCKFVNLFRSKRLQNGHFYIFNKDIIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5C4</accession>
    <name>A0A1E5R5C4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Actin patches distal protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3724</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5C4">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5C4"/>
    <dbReference type="InParanoid" id="A0A1E5R5C4"/>
    <dbReference type="OrthoDB" id="10253744at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009737">
        <property type="entry name" value="Aim32/Apd1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31902">
        <property type="entry name" value="ACTIN PATCHES DISTAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31902:SF14">
        <property type="entry name" value="ACTIN PATCHES DISTAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06999">
        <property type="entry name" value="Suc_Fer-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="unsure residue" description="D or N" evidence="1">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ82101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="400" mass="45805" checksum="B0C3333BDC116E87" modified="2017-01-18" version="1">MSAFLKKFANKVTGNSPDKLSTKELDEINECLDLCSDETKHACFNGEGEGEGEGEGEGNKNDTNYAREQKVWDTMKMDNETKLYGSSSPSNLQIIIPTSVHDWKHDAMTETYSDNSVPYQVSQFIKQHSNNDNEDGFKITGNCSSLPIQDILDIEYMKMRKSDLLLLPYFIWIKGITYNDVVKKLDAMLPFLQXYSNSNNNDKDQHPHTMGSKQELIAKLQTIHPEVYSSDKQCCVLLCSHTTRDKRCGKTAPILKKTFESELIKYNVEDEEEINIPTHNLYVPNDQDPMSKNKVMIVFINHIGGHKFVANCQIYLQKGLKVTKHLSEEKSTSGDGTVEQKTVKESVTEEGQEKLFVWFARISPMDVPMIVKDLIVQCIMGEPLKWERPEKVRCIKRFRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5N8</accession>
    <name>A0A1E5R5N8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3819</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5N8">
        <property type="interactions" value="639"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5N8"/>
    <dbReference type="InParanoid" id="A0A1E5R5N8"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF81">
        <property type="entry name" value="PROTEIN ABHD13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="2">
        <location>
            <begin position="110"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ81853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="288" mass="33291" checksum="14A2702B25B97760" modified="2017-01-18" version="1">MNFPKVFKMVFGGIVTTLSVSVAALYLYQNKLVYPSWAGKAKEYVEKPTSVSYPGLNFKEFYLKTPDNIRLQCYDFPNPESKTTIIILRPNAGNIGHSLAKINYLYHEYNASVFIFSYRGYGYSEGTPSEQGIKTDVECIMDHIEQDEFHKQRKKVLLGTSIGGAVAIHIAYRYHHMVEGVILENTFLSVRKVIPYLIPFMQRFTSMCHEVWDSEAIISKVGPKTPFLFLNSERDEIVPXNHSLELYKLCNSSEKVLKRFPLGKHNDIVIQDGYWEAFDEFMYRYNLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7E8</accession>
    <name>A0A1E5R7E8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Protein-lysine N-methyltransferase EFM1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3486</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7E8"/>
    <dbReference type="FunCoup" id="A0A1E5R7E8">
        <property type="interactions" value="299"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7E8"/>
    <dbReference type="InParanoid" id="A0A1E5R7E8"/>
    <dbReference type="OrthoDB" id="42889at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19180">
        <property type="entry name" value="SET_SpSET10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044432">
        <property type="entry name" value="Set10/Efm1_SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF147">
        <property type="entry name" value="PROTEIN-LYSINE N-METHYLTRANSFERASE EFM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ82808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="569" mass="64982" checksum="BD207E27EEB65DA9" modified="2017-01-18" version="1">MTVTQQAVDDCVQWAVENGSKIPSEYEFKYNKLSGTSCVASKAFTSTTDPLEIHKSVVLTNTVAKHGLEKYVSEHESLGSLPPNALLKLFIVLLKKNILGAGLNFDFENGKKSDFYQPYIKVLPEDILSPLLWNDSEVALLQGSDLILKRNSILQNQIVPEFNAVLSKFSFPKDKNVEVTLQDYVWAHLIVTSRGFPAILISDDASSKNEAFLWPIVDFLNHESTANVKWNYTDPYLTFTSSTETKGIGKGQEIFNNYGDNKTTDDLLLSYGFLPTAPVQDDFTMITLRVPSKEVFSECEKTHYIKFDEVLESSTDNSCIVKFKAYGKFNLPINLIKFFSYLCKLSKETFLSVRSTLEGLDQLLKIMDSKLKFYKKKLQSTKQCRETVISLIKNYKNVMKQIFQSNIDIIQQFEKNLIATSLKNDTMKSFKTCIKEDTVFQNSLLLVFGTFDYNKLKEQNLLQQILLLYIVKQGKTPANQALGTTNTIIKNTFIQTGKEIEVDTEDLKEYTALYNTFFPSLERKIPEVYGNGWTLQDFVVADTVVDRTCWQRPINQEIFFIEQKPYSLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7W7</accession>
    <name>A0A1E5R7W7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GrpE protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3399</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the GrpE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7W7"/>
    <dbReference type="FunCoup" id="A0A1E5R7W7">
        <property type="interactions" value="870"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7W7"/>
    <dbReference type="InParanoid" id="A0A1E5R7W7"/>
    <dbReference type="OrthoDB" id="201635at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00446">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.22.10:FF:000002">
        <property type="entry name" value="GrpE protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.20.20:FF:000011">
        <property type="entry name" value="GrpE protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.22.10">
        <property type="entry name" value="Head domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01151">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000740">
        <property type="entry name" value="GrpE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013805">
        <property type="entry name" value="GrpE_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009012">
        <property type="entry name" value="GrpE_head"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237">
        <property type="entry name" value="GRPE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237:SF23">
        <property type="entry name" value="GRPE PROTEIN HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01025">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00773">
        <property type="entry name" value="GRPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58014">
        <property type="entry name" value="Coiled-coil domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51064">
        <property type="entry name" value="Head domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01071">
        <property type="entry name" value="GRPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="42"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="43"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ83006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="234" mass="26706" checksum="90A70ED007A7CB65" modified="2017-01-18" version="1">MRPASRLALLRPTPSFVTRARAFNNVSTRALFASSARFYSSAETEKNNTKEEGKNEEAETANASEKTPEELKMEEFNKEIEICKKDARKFKDHYARAVADFRNLQEITKKDVQKARDFALQKFAKDLLESVDNFGHALNAFKPETIEKSKELKDLYDGVQLTKDVFEKTLAKHGIEKIDPVDQEFDPNLHEATFEFPDPNKKPNTVFHVQQVGYSLNGRVIRPAKVGIVKDPEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7Z8</accession>
    <name>A0A1E5R7Z8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="7">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3163</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="7">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7Z8"/>
    <dbReference type="FunCoup" id="A0A1E5R7Z8">
        <property type="interactions" value="229"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7Z8"/>
    <dbReference type="InParanoid" id="A0A1E5R7Z8"/>
    <dbReference type="OrthoDB" id="193716at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070013">
        <property type="term" value="C:intracellular organelle lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="145"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="368"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="74"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="615"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="626"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="638"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="656"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="690"/>
            <end position="737"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ83032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="737" mass="84211" checksum="4408D5D5A6832622" modified="2017-01-18" version="1">MLANKLVYLRTAVKSSVLRTVPCVVSKNSYLRFSTVNSRVWNEAANIAKSPEFANVQDAQASVATTAAKIPTPLSSSPLNTLETKENDISQENGNENDVSQEKDYEVTTFQGLRDNKLIDPLISKALSRTGFVDMTPVQSKTLKPILENENYDIIARAKTGTGKTLAFLIPIFESLCKTRRESQYMVKSVIVAPTRDLALQIEEEIKKVFYKNNYGLEKFPSMSLIGGVKMYDQIKKMRKIRPNIVVCTPGRLLDMLSDERLANEFFRFVDFKIFDEADRLLEIGFKEDMEQISDLLNTLSAKPENHIRSLLFSATFSKEVRKLSQSIMNKPDFKFIDTVDKNEPEVQEKIDQKLVVVDSTEKLILESLKYLTDKVANKETGKYIVFVPVTTFTSVFANLFKNILYDNHLLNNANINVLHGKLQQERRTKTVQRFKKSDDRMVNFLFCTDVGARGMDYPNINEVIQISLPSETANYIHRIGRTARGSSEKGESIAFVTKPEQKFFDVLAREKKVFVDNKIEDFEPFEDKYNIAESVKSEMVKNVDAWNETFYSLLGFHRSSVSEFKTNMTALAQDCCISFDKLTGGTKLHVSPSSINRIFGNQLLRQIVEYIDYDGPLPRDEPSRSRYGGNNSRNRFNRFDNDGQKKSRDYNNDTRSNSGFSNRSGFSDRGNSSNRNNYSSRNNYSDRNNYSDRNSRTEGRPRTEGRPRSFEGRPRSFEGNERSYGNKDTRGSRDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R951</accession>
    <name>A0A1E5R951_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3443</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R951"/>
    <dbReference type="InParanoid" id="A0A1E5R951"/>
    <dbReference type="OrthoDB" id="4035871at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ83063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="424" mass="47927" checksum="6DA11A1119324FE1" modified="2017-01-18" version="1">MLRGKHGLKASSRVLMLKNGTILHYVPVDPSPMPWKEVVGALHKLNRKSLKPKFVKSVTSNFRKAFFSQYFHDDPRSEEILRTLVKKTKSSGVKELSLMLRQGKVRDDLLYETLVSVILVNLAVPPTFDKFDSFINTTSNKEILTSMPEPPKKPEIKKDAISPALSPRFVGDSSNTDIQKKASIPKEALNINLETSKNPAALQDVLLQDFIPENDESSKDFSSSSEKSLEPINLDSLQDFLSNAEAKKKVLTFFENSKVPFPKTFDWNQNSFEVPKRQKVSLSDVFFSNKSKYVDAGYSKQRMPDLAQDKQYLMYDVSENTEYILKPVDLLMIKLEKKDLFEVINGCTSKQPQHILEIINTYLLDDMTKWNVYGTVAGSSNKIVFAKELPKQRASKSPKLNTALKITTVVLLVSLASIGLYDFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA27</accession>
    <name>A0A1E5RA27_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">beta-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2848</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA27"/>
    <dbReference type="InParanoid" id="A0A1E5RA27"/>
    <dbReference type="OrthoDB" id="47059at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000495">
        <property type="entry name" value="Periplasmic beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050288">
        <property type="entry name" value="Cellulose_deg_GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011658">
        <property type="entry name" value="PA14_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715">
        <property type="entry name" value="BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715:SF27">
        <property type="entry name" value="BETA-GLUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07691">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00758">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PA14" evidence="6">
        <location>
            <begin position="413"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ83752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="849" mass="94219" checksum="B10DC7BBC24D49B6" modified="2017-01-18" version="1">MGVDFNVDYLLGELTLHEKIKLLAAEDWWHTAAITRLGIPSIRVSDGPNGIRGTKFFDSVPSACFPNGTALASSFNEDLLVRAGELMALESKHKGAKVILGPTTNIQRGPLGGRGFESFSEDPYLSGVATAAVVNGIQKSNEIAATVKHFVCNDLEHERYSSNSIVSERALREIYLEPFRLAVKKSDPKLFMTAYNKVNGIHCSSSKKLIQNILRKEWKSNALVVSDWFGNSDTVDSIKNGLDIEFPGPTRFRKPDIVSHLLNCKAETRDGEQFSIEHVDARARKVLELVKYFVDVEGTTELPTNEDDKNNTPETAKLLREIGNECIVLLKNDKNVLPLNKKDDILVIGPNAKALNASGGGSAALNGYYTISPLQGIANAVGKKLEDIPYIKGCENHKTLSNLVEQCTNDVDLTQKGFAMSFYTKPAKERGDEKPFDDRIITNSYIALYDYDHEMIDHKTNLFYCDIQGYFTPEESGVYEFQCQVLGTALVYIDNKLVINNKENQVLGDFGFGSGTIPVSAFLNLEKGRKYKIQIDYGSGPTSVLCKNREESALKVGVNKVIDEQEEIKKAAELASKHDKVILVIGLNGEIESEXYDRXNMQLPRRTNDLVTAVLKANPNTVIVNQSGTPVEFPWLQQATTLLQAYYGGNELGNSIADVVFGDANPSGKLSLSWPLKNEDNPAYLNFKTVMGRVLYGEDIYVGYRFYEKLQRQVAYPFGHGLSYTTFKFNELXVSGDDESLKVELSVANTGKVDGKEVVQVYVARTSPSAVPRPVKELKKFKKVALKAGESAQVELTLSVKDSCSYFDEFHNQWHLEAGKYQVLVGSSSDDIHLIGDFEVKESXFWLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA86</accession>
    <name>A0A1E5RA86_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2836</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RA86"/>
    <dbReference type="OrthoDB" id="3972422at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ83804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="605" mass="71212" checksum="405FF7F1F64E2EE4" modified="2017-01-18" version="1">MFSKTKQRIILKTTLVSFLLLLTGVFVLQQHRKIKLHDENQVSYNLMSSSRYNDLESFRKEDQDIYGQYLKYLRDPVISVLLKFKEPQKNYDDTLTESQKAPEHIKNPNVNHFKNTLYNYSDXQNQKYFKFPFHTLEYPYFDLKAKQQVLVETNGKYRPQHTYKNGKNCIVFFLSDGGSPDRLLSSINSFEENWNXKYHYPYVIVTGFYISENFIYERLLTLDIENEITVIRTTDIKNDAYVEGNWDYLVDGYANYLKGGYYKNKDDSLNYHISKLYPEAYYPKTGSDRSQDKLTAAYSFMFEEYGXASIEMTNFASYDWMNLEYFKKSDNFLYVKPGMIIREPVDEDLFEGLQTGLNAKFIDFKRSPGFKNTLQQQIACDVYVATKNNLSTFFKSDNELPNEQLNAEQKLEPKADTSKAYRLHLLSKLCKNELFNGLTLSLHNSMFVSKFNIFRSMEYELFFKYMLLTRATEDDFVSANDIMSLFLALFNAQDDENTEYRQDLEMFLDIDVTLKHNLDKDLAASFNEFGCCPLFRVDEESESCVGDTRFFMYYVNSEKASIEFYHFGEAINFVKQQCIKKNYDNEKCAESLNRLISSNIQSMFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAG2</accession>
    <name>A0A1E5RAG2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Casein kinase II subunit alpha</fullName>
            <shortName evidence="11">CK II alpha</shortName>
            <ecNumber evidence="11">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g2768</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Phosphorylates YTA7 during S-phase to promote transcription of histones.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="11"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAG2"/>
    <dbReference type="FunCoup" id="A0A1E5RAG2">
        <property type="interactions" value="1094"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAG2"/>
    <dbReference type="InParanoid" id="A0A1E5RAG2"/>
    <dbReference type="OrthoDB" id="10254671at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032545">
        <property type="term" value="C:CURI complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007535">
        <property type="term" value="P:donor selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060962">
        <property type="term" value="P:regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006356">
        <property type="term" value="P:regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006359">
        <property type="term" value="P:regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14132">
        <property type="entry name" value="STKc_CK2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000459">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000088">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045216">
        <property type="entry name" value="CK2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="69"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="150"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="158"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ83861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="437" mass="51698" checksum="E4D7C6F582BA4FF6" modified="2017-01-18" version="1">MATPTTPVKSVARCYAYVNKRKPQSYWDYENYKISYSENDQNTYYSGYNASLEEMESVRGDVPCSKPKYEVINKVGKGKYSDVFKGLDIKSSTFVVIKMLKPVRRKRIQRELKILQNLISYASKDCIFDEESYLEGSLDQLFDNFDKHDTEETEENDEAHGNEDTSMGTKEDGSTQASLVTNNLNEYRNRLYYKKSHNGYENIITLHNIMKDPVSKSTQLVFEHVENVDFRKLYPTFTDYDIRFYMFELLKALDYCHSMGIMHRDVKPYNVMIDHPNRKLRLIDWGLAEFYHAGTEYNVRVASRVFKGPELLVDYRLYDYSLDMWSFGAMLAAMCFKKEPFFHGTDNIDQLIKIVRVLGSDGLYAYLEKYKIRLPKEFADMEYYLRRPWKRFINDTNKHLCGNVQFLDFIDNLLVFDHQKRFTAREAMMHSWFDPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAI2</accession>
    <name>A0A1E5RAI2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 17">Delta-aminolevulinic acid dehydratase</fullName>
            <ecNumber evidence="5 17">4.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AWRI3579_g2571</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 17">
            <text>2 5-aminolevulinate = porphobilinogen + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="4.2.1.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 17">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the ALAD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.24" evidence="5 17"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAI2"/>
    <dbReference type="FunCoup" id="A0A1E5RAI2">
        <property type="interactions" value="625"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAI2"/>
    <dbReference type="InParanoid" id="A0A1E5RAI2"/>
    <dbReference type="OrthoDB" id="1530at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00318"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004655">
        <property type="term" value="F:porphobilinogen synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04824">
        <property type="entry name" value="eu_ALAD_PBGS_cysteine_rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000048">
        <property type="entry name" value="Delta-aminolevulinic acid dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001731">
        <property type="entry name" value="ALAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030656">
        <property type="entry name" value="ALAD_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006762">
        <property type="entry name" value="PRK09283.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458">
        <property type="entry name" value="DELTA-AMINOLEVULINIC ACID DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458:SF0">
        <property type="entry name" value="DELTA-AMINOLEVULINIC ACID DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00490">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001415">
        <property type="entry name" value="Porphbilin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00144">
        <property type="entry name" value="DALDHYDRTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01004">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00169">
        <property type="entry name" value="D_ALA_DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="10 17" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 17" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="14">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="14">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OEJ83915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="341" mass="37779" checksum="A37F5B2260E34C0D" modified="2017-01-18" version="1">MSKHTAEFLPANEPDLKSILQGGYNHPLLREWQSERQLTKNMFIFPIFISDSPDEETKIDSLPNIKRYGINKIVSYLKPLVAKGLKSVIIFGVPMKDGCKDDVGSAADDPEGPVIQCIKLLRKELPELYLMVDVCLCEYTSHGHCGVLYEDGTLNRDLSVRRIAAVATNYAKAGAHSVAPSDMMDGRIKDIKLGLMNAGLAHKTFVMSYSAKFSGNLYGPFRDAACSAPSSGDRKCYQLPPAGRGLAVRALRRDMDEGADGIIIKPSTFYLDIMRDASEICRDLPICAYHVSGEYAMLHAAAEKNVVDLKTIAFEAHYGFLRAGARLIISYFTPEFLEWLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAS9</accession>
    <name>A0A1E5RAS9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cell division control protein 48</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2914</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAS9"/>
    <dbReference type="FunCoup" id="A0A1E5RAS9">
        <property type="interactions" value="1437"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAS9"/>
    <dbReference type="InParanoid" id="A0A1E5RAS9"/>
    <dbReference type="OrthoDB" id="27435at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051228">
        <property type="term" value="P:mitotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071630">
        <property type="term" value="P:nuclear protein quality control by the ubiquitin-proteasome system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19519">
        <property type="entry name" value="RecA-like_CDC48_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19528">
        <property type="entry name" value="RecA-like_CDC48_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000004">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.10:FF:000001">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000003">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000048">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005938">
        <property type="entry name" value="AAA_ATPase_CDC48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01243">
        <property type="entry name" value="CDC48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="7">
        <location>
            <begin position="35"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48" evidence="6">
        <location>
            <begin position="135"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="247"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="520"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="802"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="802"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="828"/>
            <end position="837"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ84020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="837" mass="91638" checksum="A3354FEFED19EF38" modified="2017-01-18" version="1">MVNEKKPLLDASGGDQAKEDATATAILRRKKKPNQLLVDDAVNDDNSVVAINSNTMDTLELFRGDTVLVKGKKRKDTVLIVLIDDELEDGMCRVNRVVRNNLRIRLGDVVTIHPCPDIKYASRISVLPIADTIEGLSGNLFDVFLKPYFVEAYRPVRKGDHFIVRGGMRAVEFKVVDVEPEEYAVVAQDTVIHYEGEPINREDEENNINEVGYDDIGGVRKQMAQIRELVELPLRHPQLFKAVGIKPPKGILMYGPPGTGKTLMARAVANETGAFFFLINGPEVMSKMAGESESNLRKAFEEAEKNSPSIIFIDEIDSIAPKRDKTNGEVERRVVSQLLTLMDGMKARSNIVVIAATNRPNSIDPALRRFGRFDREVDIGVPDATGRLEILRIHTKNMKLADDVDLDVLASETHGYVGADMASLCSEAAMQQIREKMDIIDLDEDEIDAEVLDSLGVTMDNFRFALGNSNPSALRETVVQNVNVSWEDIGGLDEIKQELKETVEYPVLHPDQYLKFGLSPSKGVLFYGPPGTGKTLLAKAVASQVSANFISVKGPELLSMYFGESESNIRDIFDKARAAAPTVVFLDELDSIAKARGGSIGDAGGASDRVVNQLLTEMDGMNSKKNVFVIGATNRPDQIDPAILRPGRLDQLIYVPLPDERGRLSILEAQLRKTPLEPGLDLNTISKATKGFTGADLSYIVQRAAKYAIKESINKKIKLESEKEKDPATGDVKMEDDAEEEVDPVPYITMGHFAEAMKTAKASVSEAELRRYEAYRMKYSASSIVSSSAAAVASEALNFNNTSNANAGGNNNNANNNNATGGGAAFGGDEEDDDLYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB19</accession>
    <name>A0A1E5RB19_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sulfhydryl oxidase</fullName>
            <ecNumber evidence="6">1.8.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3067</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2</text>
            <dbReference type="Rhea" id="RHEA:17357"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16520"/>
            <dbReference type="ChEBI" id="CHEBI:17412"/>
            <dbReference type="EC" id="1.8.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.3.2" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB19">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB19"/>
    <dbReference type="InParanoid" id="A0A1E5RB19"/>
    <dbReference type="OrthoDB" id="59470at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016971">
        <property type="term" value="F:flavin-dependent sulfhydryl oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.310:FF:000002">
        <property type="entry name" value="Sulfhydryl oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.310">
        <property type="entry name" value="ERV/ALR sulfhydryl oxidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039799">
        <property type="entry name" value="ALR/ERV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036774">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017905">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645">
        <property type="entry name" value="ALR/ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645:SF1">
        <property type="entry name" value="FAD-LINKED SULFHYDRYL OXIDASE ERV2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04777">
        <property type="entry name" value="Evr1_Alr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69000">
        <property type="entry name" value="FAD-dependent thiol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51324">
        <property type="entry name" value="ERV_ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 6" id="KW-0274">FAD</keyword>
    <keyword evidence="2 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERV/ALR sulfhydryl oxidase" evidence="7">
        <location>
            <begin position="72"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU371123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ84097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="217" mass="24513" checksum="DD929205C7DBE5C5" modified="2017-01-18" version="1">MKSVTINKHKIITLLASIFLVTLWFHFSSPSLSYQTANEALNYTPKKSDTKTXLSKVDGGKDDIYSNVIMPKMPDQKAKQELGRTSWKYFHTVLARFPDEPSSDERQKLDTFLHLFAELYPCGECSYHFVELLKKYPPQTSSRFVAAMWGCHVHNVVNQFLKKDEYDCSTILEDYDCGCGDEATAEAAKTVDSKVEKNGALTKGSTITLEKEGKQHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB39</accession>
    <name>A0A1E5RB39_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Something about silencing protein 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2934</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAS10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB39">
        <property type="interactions" value="420"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB39"/>
    <dbReference type="InParanoid" id="A0A1E5RB39"/>
    <dbReference type="OrthoDB" id="1924577at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018972">
        <property type="entry name" value="Sas10_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237:SF8">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09368">
        <property type="entry name" value="Sas10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sas10 C-terminal" evidence="5">
        <location>
            <begin position="539"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="31"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="339"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="455"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="554"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="43"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="94"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="353"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="396"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="455"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="556"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF09368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ84115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="615" mass="70461" checksum="A821B90823F22522" modified="2017-01-18" version="1">MARGGSNDXHPYGLNEVDDFAQKRGEILLSQSTLNKKQHSYDSDEELADNGEEEIMGIDESDDDNEEDASDYEVKTEDVHKKVFGRRMEPTQNSDEEDFDGEGDEEDLQWGTTKNEYYGADDLDDDDEAKEIEKEALRQQKLHLDELHMQDFLDDEVENEWVKKAKDYDMEKFKESTSMKSTDASTEDIKNIFQMDEESKKDFIKTSFPEFFPLSSEFAKLNSKLEELNANPNAQDSHVLNIKKSALNAYISVIASYFALFLNELKNNIDEFYTMKDHPVMESILTLKEIWRQADELPNELEMPDQVMNSEDGQEIDQEHSDGELDQVSELDEDILAATVPIDNEGESSSXDEGSEDDDGSEEEDGEQQQQTDFGIDISASRVPQNGNKKTRDMDNDFLETDINDVDAAEKRARKKTLRFYTSKIDQADLKKLERFKGDDDIPYKERLFERQQRLLEEARKRGQNNGKDDEHDLDTGDYNSDDEKVASSVNTGGQKDDYYNQVKNKKLYKKLARSKAHEEAIIASKEGKLAEAAENIGEGGKRAINYQILKNKGLTPKRHKDNRNSRVKKRKKYEQAQKKLKSVRQVYSGGQSGAYEGEKTGIKKNLVKSVKFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB73</accession>
    <name>A0A1E5RB73_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA polyadenylation factor FIP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3075</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FIP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB73"/>
    <dbReference type="InParanoid" id="A0A1E5RB73"/>
    <dbReference type="OrthoDB" id="1917198at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007854">
        <property type="entry name" value="Fip1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051187">
        <property type="entry name" value="Pre-mRNA_3'-end_processing_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13484">
        <property type="entry name" value="FIP1-LIKE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13484:SF0">
        <property type="entry name" value="PRE-MRNA 3'-END-PROCESSING FACTOR FIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05182">
        <property type="entry name" value="Fip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pre-mRNA polyadenylation factor Fip1" evidence="7">
        <location>
            <begin position="196"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="344"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="66"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="91"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="141"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="268"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ84147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="405" mass="44939" checksum="F4628449141D7982" modified="2017-01-18" version="1">MAGSMDVHGNEKHQIDDEDDEQFLYGDDNNNNNNNNNDTSDNKTTKKRKLSGEVVQNDDLNDLYSAENNNKASTSRSPPKKQMKLSNEKGQEEEEGEEGEGEESGSESEYETDESDDVQFIIGTGSNPQQLSSKSAKDAKSVAAGTTTTTTTTAAAASEDTSKETQQSTLQQSRISANPLIDLDAKGLYEGEPIEEVDPEIIKEKPWRGANAKMSDYFNYGFNEATWMEYLHRQEKLLTEYNPQKLLQKLLFLQQQGKLGNAQDTNKNFSSGTNYNNSNGENSEVNNLAGTLAYQQRQQQQDPPLPESVKMNTTMHGSNTNMNMVNGMPPMNAMPMGNMNNMSNNNNNNNNXNNXNNNNMNNNNMNRNIPGQPPFPMPMFGGFPPIPFPFNVPMGNMSPPPPPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB85</accession>
    <name>A0A1E5RB85_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial</fullName>
            <ecNumber evidence="5">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Succinyl-CoA synthetase subunit alpha</fullName>
            <shortName evidence="5">SCS-alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2657</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>succinate + ATP + CoA = succinyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 5">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RB85"/>
    <dbReference type="FunCoup" id="A0A1E5RB85">
        <property type="interactions" value="791"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB85"/>
    <dbReference type="InParanoid" id="A0A1E5RB85"/>
    <dbReference type="OrthoDB" id="1664372at2759"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009361">
        <property type="term" value="C:succinate-CoA ligase complex (ADP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004776">
        <property type="term" value="F:succinate-CoA ligase (GDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000002">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01988">
        <property type="entry name" value="Succ_CoA_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005810">
        <property type="entry name" value="CoA_lig_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004230">
        <property type="entry name" value="PRK05678.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117:SF2">
        <property type="entry name" value="SUCCINATE--COA LIGASE [ADP_GDP-FORMING] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117">
        <property type="entry name" value="SUCCINYL-COA LIGASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001553">
        <property type="entry name" value="SucCS_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01798">
        <property type="entry name" value="SCOASYNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="CoA-binding" evidence="7">
        <location>
            <begin position="36"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="5 6">
        <location>
            <position position="285"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="48"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="127"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit beta</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001553-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ84160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="330" mass="34595" checksum="BE74656E97537FA3" modified="2017-01-18" version="1">MLRFTNKTKLVAQMGQRLYSSRQSTVYDKTIGNLALPDTTKVIIQGFTGKQATFHSKISMDYGTNIVGGINPKKAGQTHLDRPVFATVKEAMAQTGATVSGIFVPPPIAANAIKEAIEAEIPLAVCITEGIPQHDSLYIAEMLASQSKTRLVGPNCPGIINPASKVRVGIQPTSIFQEGKIGIVSKSGTLTYEAVQQTTLTGLGQTIVIGMGGDAFPGTDFIDALKYFQNDPKTKGLILLGEIGGLAEIEAAQFITEMGLSDKMPIASFIAGEATAGIKGKRFGHAGAIASGADDTAAAKKTALRNAGVAVVDSPGMLGQALLQEFSKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBI8</accession>
    <name>A0A1E5RBI8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Target of rapamycin complex 2 subunit AVO2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2492</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBI8">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBI8"/>
    <dbReference type="InParanoid" id="A0A1E5RBI8"/>
    <dbReference type="OrthoDB" id="823504at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="86"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="120"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="368"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="396"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="415"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="425"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ83933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="475" mass="52788" checksum="019381723A14AFBA" modified="2017-01-18" version="1">MILRRYPDLLYCIDSSNGWSLLHYASFHGRYLICVHLIQLRCERDEIRTTFLNNTAVHLALINGDEQTIHLLLQHFPLCINMKGQDGYTPIHFCCRYNYYQVLSLLISCGAKLDLPDDKHGDYPLHVAIKYGNLDVAKILIQNGQQDSIYNKMHMKPSDVGYSFEIIKQYLKLLKEEETRETQLIKINTNITTTSLNTMGTAHSYMSNNKNNNKNNHSIGSKTPNLKSDGAGTLISNNPNDYGSTSRLAKPVKATAHLKSASKLSNLQIHPISTKNNSTNVSNTSSGDEIEVMVNNTPVIVPPSAIIQTSSDNANYGGYSSNNGKPSNNTNGIQIRTMSPSPIVINTPNNMNNARRFPSSVSFLDTPXTSSSFDIQNEKENGHSFGSPLELKKTGDLQSKSSMNFLRSNDTPKSTLEHDEELKSLKSTPHSPSFSDLGDESSCNIVAKKDSQDIDSRKKSSILLNVPIAKVREHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBL6</accession>
    <name>A0A1E5RBL6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Peroxisomal membrane protein PEX30</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2587</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBL6"/>
    <dbReference type="FunCoup" id="A0A1E5RBL6">
        <property type="interactions" value="68"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBL6"/>
    <dbReference type="InParanoid" id="A0A1E5RBL6"/>
    <dbReference type="OrthoDB" id="5586090at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006614">
        <property type="entry name" value="Peroxin/Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052646">
        <property type="entry name" value="Peroxisomal_PEX28-32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010482">
        <property type="entry name" value="TECPR1-like_DysF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31679">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX30-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31679:SF2">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX30-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06398">
        <property type="entry name" value="Pex24p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00694">
        <property type="entry name" value="DysFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00693">
        <property type="entry name" value="DysFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Peroxin/Ferlin" evidence="8">
        <location>
            <begin position="385"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Peroxin/Ferlin" evidence="9">
        <location>
            <begin position="477"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="575"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="49"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="64"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="575"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="626"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="652"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ83963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="684" mass="76183" checksum="4EA34462E77A7C9B" modified="2017-01-18" version="1">MQEDASGLGHTLENPKMTETVTSGSRVASSSSLPSYSGSTSSSAPLSNNDKENQDVAPKEAEKLSSTIQANSTSTSSHANIDVQATKRAKLVNPDPVNVDRLGFIHRNKSNSNKPLRGVLKNNSKGVHSNPADHQEIIQSSPLLTSTPPSISRALIRLYPYLIVFDKILSLLTWTGEDIWGSVLLLCIYITINLYFQNIVTYFGHLTCVLLLWLYSNLDRIVGTEMEERPTLDDIIHTVSSVSSKFDLLLSPIAVLTTSDIKRLLITTVFLSPIYMILTVFFLPKGNLMLLLGVIGLSYHSSWSRVTRKMLWKSKTIRLFCFYITGLDIDGIKSYKALKGKTSGHNSLSSTFATGGSTSTGLFSTVQKKLLKSNRENESSYDNKPIRFTYVLYENQRRWLGIGWTANMLSYERSCWTDEFLNEAPDPDNFKLPDDSSSGMIWRWIDKTWRLDISNDGAIQVPASRSKTTASPSADDGFIYYDNTWKKPAIDDTYSKYTRRRRWVRTAELIKVSDTPMPHQPSLSEATIESQGVSVQVASANTTGADRKNLSDAANLHQPGNRLSANNIVDGMEKSQGTLQSQKPRKVSFSTTENVIPYGQDYMTSNRNRDSAHFPTTPEGSENETSHNGASSSSEILQNLQEEPDIKHRKQPSTNNIKYTFSSSNNNSELEPQNSSLNTTNDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBQ3</accession>
    <name>A0A1E5RBQ3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2696</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBQ3"/>
    <dbReference type="InParanoid" id="A0A1E5RBQ3"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="477"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="536"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="623"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="283"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="477"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="539"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="628"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="688"/>
            <end position="699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="699" mass="79801" checksum="39F630BB44176C6C" modified="2017-01-18" version="1">MWTFTYKHETRGSRSVADTLQNTDIAKVIQGNTWYFAGRQPGISEKVKQAEFEGRKIELVEILGDKTVSKLHLRFFLKNDDTLEVDNFGASTSTGTSNVKTLTRWTLNATDARKLFLILGKNLFFKFKWTSNDEPIAKKSNGPMESGLEVQKPNNISALKRKSYKTDISSLIDFDFTPRDIEKDKPKVDDIKDLSSLQNQDYFAPAIESNTNLPRKTTKLGKPNNIMSLFDDIDVTPVDIKRPSETDAQSASARPDIQSIENRKTPPLVLAERQELKRKKEQLQQQQQQQQQQQRHEKDGTVLSAEKNGTSIETNGSESLPAAHGILPQRDFLMHPITKFNKYDVDSPQVTAIRNLMQTNKQQENESFGVPVSEIKEDINIKPKVSGFEIDTRYCNSYLINKNKNWPARKNFKQFVKVLGKHQAAEQSIRDTNQHETKKLYQETAWRITDQEFITVTPKFNDLDMVSNISMSKPARAAPSHSVASSKSASVNLETQSQREKRNYTTIAQPPKEVLNKKAVLFVENENENEIEIENMNEIGDKNRETATAKREPHQSSRSKKKIITNSDEESDAELLEILKSTGSNTKRRRLRTGMRGNIEQEAINNLNYMDSDSDLEEFHFAKKPRSVRSSPVKNSSAPGSNKKPLKTSETTTFEKHNSSKTQNDSARRLTKRRKMVDDQLQRGDQNWSDEEEVFSFKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RCP6</accession>
    <name>A0A1E5RCP6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UBX domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2494</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RCP6">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RCP6"/>
    <dbReference type="InParanoid" id="A0A1E5RCP6"/>
    <dbReference type="OrthoDB" id="440781at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01767">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021569">
        <property type="entry name" value="TUG-UBL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46467">
        <property type="entry name" value="TETHER CONTAINING UBX DOMAIN FOR GLUT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46467:SF1">
        <property type="entry name" value="TETHER CONTAINING UBX DOMAIN FOR GLUT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11470">
        <property type="entry name" value="TUG-UBL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="2">
        <location>
            <begin position="274"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="388"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="391"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="430" mass="49436" checksum="A7498747F0C74C45" modified="2017-01-18" version="1">MSGTVSIEHNYNSIKCKYEAKTTLNQVLDEALIRFDLSKQEGPYALLYKAKSLNLSYSFRLLNLPQGAKLVLKKMDFSNTQIRVKFQIQPLNTSKIFKVSPIAKINEFLAQVIATTDNTQYKLQVFNQVYTEKNISESTTFLDLGITEDMAIRVTXLEKQPEKNLLNKVGEKSSIETSTKNEVSLSETSNEKTAPVAVAQKTTHRPIAFTPSSNPEKDIKKFINXHANENEYEVDVEDAKKYQHMLSKMANPLLQKREELEKATRKKDDFVNGHNTGFCDVRFRFPDRSILQTTFQAEETTHALYEFINANIRYENMQYKIHLSNPYTHIINSKELKLVNDLKFSTRTLVVLETDKPGPYFNKNIEVHQLDSFKEETDSLPVATASIDNDTTMKEQFQEDDKKAQEATKKDTKIDGFEKKISKFLRLSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDH3</accession>
    <name>A0A1E5RDH3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1934</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the bacterial ribosomal protein bL36 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDH3"/>
    <dbReference type="STRING" id="56408.A0A1E5RDH3"/>
    <dbReference type="InParanoid" id="A0A1E5RDH3"/>
    <dbReference type="OrthoDB" id="10265903at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00251">
        <property type="entry name" value="Ribosomal_bL36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052143">
        <property type="entry name" value="Mitoribosomal_bL36m"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000473">
        <property type="entry name" value="Ribosomal_bL36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035977">
        <property type="entry name" value="Ribosomal_bL36_sp"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01022">
        <property type="entry name" value="rpmJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46909">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L36, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46909:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL36M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00444">
        <property type="entry name" value="Ribosomal_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57840">
        <property type="entry name" value="Ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ84947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="96" mass="11013" checksum="448B2B12728A00D0" modified="2017-01-18" version="1">MFQNQLFKLAARLGKPSTSRLLNGVSHNLQHGYGNASSLSPLSMKQRSLYQFPTLARTFKVKTAVKKMCPDCYLARRKGRLWVYCRSNPKHKQKQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDJ5</accession>
    <name>A0A1E5RDJ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Phosphoinositide phospholipase C</fullName>
            <ecNumber evidence="2 8">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1795</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="2 8"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDJ5">
        <property type="interactions" value="343"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDJ5"/>
    <dbReference type="InParanoid" id="A0A1E5RDJ5"/>
    <dbReference type="OrthoDB" id="269822at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08598">
        <property type="entry name" value="PI-PLC1c_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015359">
        <property type="entry name" value="PLC_EF-hand-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF36">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE BETA-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09279">
        <property type="entry name" value="EF-hand_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6 8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="320"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="PI-PLC Y-box" evidence="11">
        <location>
            <begin position="627"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="10">
        <location>
            <begin position="744"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ84969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="902" mass="103642" checksum="B6B8423EC02765D0" modified="2017-01-18" version="1">MNRDQTSRRHSLSADNDGEEFLASQRSNDSVCSLETRSSTSSEKRSFFTPNIFQVIRRKSGVFSRKNSSFSFTDDFNKTAPPAAKKDTENIAVLRSKSLSTSGNTSSMQPLRTSSSGCLTHSYNFGTXPAQHLFQDKAYMACSKKNDTTTFEYKENALEHGAYLVRVTRKKSVNYKFTLINDQLLHWKDKYIDIDTIKDIRILETANNYREQLGVSSEKGKFWITVIYQVPPNKLKALHVYAANAEDFQSFYVTLYYKVKQRHDMFQKMAFPSSEEFSSFHWNSNKAAVSTKFNKLTQEKTHNLDFENVATICSKFNIFCSDSYLHNLYKRADLNDDGLLSHSEFQYFVKLLKNRPEVSRIMQGISKGEKYLDFDDFEKFLKDIQNEVLPANDLEMVFEKYKHEKLGVITTEGFLKFLAEQPYLFAVDCSSYYNKPLNYYFISSSHNTYLRGNQVGDSSTVESYIEALQKGCRCIEVDVWDGENGPVVCHGILTGSISLKSVMEVVRKYAFITSPYPLLLSFEVHCKDANQIIMIYLIKEYLGDLLAPKFENELPSPNSLKNKIVLKFTKRGKMYGSEGLPSSTTSLESSDDEDLLEQKSRNLKNPFHLGKKNKVAILPTLLELSNIQGIVFKNFSLIESKTPAHSFSFNEKRFQELTRNRYQEYAIDKHNRKFLMRVYPHALRYKSTNFNPIDFWRLGVQMVATNWQTNDLGQQLNNAMFLSPITSSSDWSSGYVLKPDYMLPPVKIKQMAKYRKSLQFSPLEVGIDVISAQWLHKPNFLKKDSPDALLDILTEVEFISDMKVKTTITVHNGNAVSDTKAQTKYIRENGVSPIWGTHISIELYDTGLNFVLFNVKTKNSIVLGTCCIKLHDLRKGYRQIPLFNTENGEKFIFSTLFIKVDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDN9</accession>
    <name>A0A1E5RDN9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">precorrin-2 dehydrogenase</fullName>
            <ecNumber evidence="2">1.3.1.76</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1787</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.76" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDN9">
        <property type="interactions" value="109"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDN9"/>
    <dbReference type="InParanoid" id="A0A1E5RDN9"/>
    <dbReference type="OrthoDB" id="1721126at2759"/>
    <dbReference type="UniPathway" id="UPA00262">
        <property type="reaction ID" value="UER00222"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043115">
        <property type="term" value="F:precorrin-2 dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.110">
        <property type="entry name" value="Siroheme synthase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3280.10">
        <property type="entry name" value="Siroheme synthase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028161">
        <property type="entry name" value="Met8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028162">
        <property type="entry name" value="Met8_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028281">
        <property type="entry name" value="Sirohaem_synthase_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35330">
        <property type="entry name" value="SIROHEME BIOSYNTHESIS PROTEIN MET8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35330:SF1">
        <property type="entry name" value="SIROHEME BIOSYNTHESIS PROTEIN MET8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13241">
        <property type="entry name" value="NAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14823">
        <property type="entry name" value="Sirohm_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14824">
        <property type="entry name" value="Sirohm_synth_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75615">
        <property type="entry name" value="Siroheme synthase middle domains-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Siroheme synthase central" evidence="7">
        <location>
            <begin position="179"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Siroheme biosynthesis protein Met8 C-terminal" evidence="6">
        <location>
            <begin position="217"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ85015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="291" mass="32926" checksum="4A1F15CBAC928E16" modified="2017-01-18" version="1">MSKPKSLLLAHRLSQRKILIVGGGEVAYTRLLKLDQTDCIIYLVSPAVTPEIQTKFVDTGRITWLEKLYSINDLYLCWRDLQPEQTSVTEGLDSLSLXTPNXQDLGSKTFEQLKELHEAQKTKRKQSWAFILTCLNDHIVSETIYYHSKLVFPDVPINVADNPPFCDFYFGANVELGTNGELQLMISSNGVSPRYTALLKKEIYKKFGNAEQMSLCVGSLQKLRSGIRVITPGKAAHDIEFRMSWVTRVTDAFGLEGCATMDADKLLVLYKEMFETKSLDFPADLVEQYSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDT7</accession>
    <name>A0A1E5RDT7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TBP-associated factor 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Transcription initiation factor TFIID subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2030</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDT7">
        <property type="interactions" value="1203"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDT7"/>
    <dbReference type="InParanoid" id="A0A1E5RDT7"/>
    <dbReference type="OrthoDB" id="361039at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22931">
        <property type="entry name" value="HFD_TAF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08050">
        <property type="entry name" value="TAF6C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000033">
        <property type="entry name" value="Transcription initiation factor TFIID complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.770">
        <property type="entry name" value="TAF6, C-terminal HEAT repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037796">
        <property type="entry name" value="TAF6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011442">
        <property type="entry name" value="TAF6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046344">
        <property type="entry name" value="TAF6_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004823">
        <property type="entry name" value="TAF_TATA-bd_Histone-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10221">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10221:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02969">
        <property type="entry name" value="TAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07571">
        <property type="entry name" value="TAF6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00803">
        <property type="entry name" value="TAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TATA box binding protein associated factor (TAF) histone-like fold" evidence="9">
        <location>
            <begin position="25"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="205"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="475"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="514"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="209"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="486"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="514"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="554"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="566"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="670"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="683"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="10">
        <location>
            <position position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00076308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="790" mass="87681" checksum="EEAECC1FBEA936C9" modified="2017-01-18" version="1">MAKPKQAQTAANSTITQANNSSFTVWSPQDTVRDVAESLGIGNISDDVLRSLAMDVEYRILEIIEQAVKFKRHSKRETLTSDDIAKSLKVLNVEPLYGYAPGVGVQNFTKTQVGVGPQQQNLYCLNDEEVDFDQLINEPLPPAPRIPSFTAHWLAVEGVQPAIPQNPPLTEIRHNYTPFVRGAIVTSINENSLQTVVXEEDLNEDITEKSTASTGKQQDTTPAGATVQANQTSQTGSKIAKSXIKPGSKELEVKPRVKHVLSRELQIYFEKVCEALLDQENTDAKTRAKTAALHSLKNDTGLHQLVPYFISFISEQITYNLDNLALLATILEMMYSLLCNESIFLDPYIHTLMPPILTLLLAKSIGNEDNTLEQTLGLRDFASELLNHVLXKFPKLDKTLKPRLTRTLLKSFLDTNRTMGTYYGCFRGVMVLGPETVRFFMGNLHNWAELVIPPLQXSLNSPITKPLXEVPEKEAEQNFVADNKAEPTTNEPADQDVDMTSLQTEDLATDTLAEDKNGHLNVEEDEQKNEADGEPXRETNESLLEDDNVKKETAVSSXEQTVNEIPXEKKDDINLKENEEAEXQQEIVKQPDESEPXNADSETEKQLKTQNQPVAETETETETEIPTAEADXNTPXQETEKDVEMTNASSSTGVLSEVKTTEDNAEPIGSSSNEASAENPXPKSSSDDVTQVNEKKEVPDAVANTDKVSSKFTNEEFDHLVKVIIEFLKTLEQELPPMEQAGSQPLSVEEYTNLQNQVGQVVAQTIKSFPXPTEARKLYKXIFFGTLGHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE09</accession>
    <name>A0A1E5RE09_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phenylalanine--tRNA ligase</fullName>
            <ecNumber evidence="5">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Phenylalanyl-tRNA synthetase beta subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g2372</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RE09"/>
    <dbReference type="FunCoup" id="A0A1E5RE09">
        <property type="interactions" value="1115"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE09"/>
    <dbReference type="InParanoid" id="A0A1E5RE09"/>
    <dbReference type="OrthoDB" id="1698572at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.40.10:FF:000002">
        <property type="entry name" value="phenylalanine--tRNA ligase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.10:FF:000004">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000052">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="entry name" value="Phenylalanyl-trna Synthetase, Chain B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004531">
        <property type="entry name" value="Phe-tRNA-synth_IIc_bsu_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040659">
        <property type="entry name" value="PhetRS_B1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00471">
        <property type="entry name" value="pheT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947:SF0">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE BETA CHAIN AND LEUCINE-RICH REPEAT-CONTAINING PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18262">
        <property type="entry name" value="PhetRS_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56037">
        <property type="entry name" value="PheT/TilS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B5" evidence="16">
        <location>
            <begin position="292"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ85142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="594" mass="66721" checksum="94141E27405D9A79" modified="2017-01-18" version="1">MPTVSVYKKDLFDLLGKQYEDQEFDDLCFDFGLEVDEDTTEEALKAGTEPELKIEIGANRYDLLCIEGIAQSLNEYLGNSTTPEYKLTKPTTKLVIDKSTEQIRPYAAAAILRNINFDAKSYESFIALQDKLHSNICRNRSLVAMGTHDLDSISGPFHYKALPKNEFEFIPLNQSKKMTGQQIVDFYNTPEQKNNLGRFTHIIQDSPVYPVILDSKDTVCSLPPLINSEHSKISVDTKNVFIEITATDRTKAEIVLNQLVAMFSRYCDDKFTVEPVEVVSEHNGESRITPNFALRYMPVSKRYINSALGLSLSSEEIAKYLEKMSLKAKPSSSDEDLLDVTIPITRPDVLHPCDIMEDAGIGYGFNNVPKDGELSNSSFVGTPLPINKVSDFYRIACSQAGWAEVMPLTLCSHDENFKFLRQEDDLTKVVKLANPKTSEYQVVRTTLLPGILKTVKENKKHSLPIKVFESGDVVFKNDKLERKAYNERHWAAIYVGKSSGFEIIQGLLAKIMQTFRTPWIADFGAASSGRGYWIEEDANNTYFPGRGARVMFRAKEGASPINVGSLGALHPEVMMNFEIPYAGSYVELNAETFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE44</accession>
    <name>A0A1E5RE44_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Hsp70 nucleotide exchange factor FES1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Hsp70 nucleotide exchange factor fes1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2161</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FES1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE44">
        <property type="interactions" value="215"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE44"/>
    <dbReference type="InParanoid" id="A0A1E5RE44"/>
    <dbReference type="OrthoDB" id="10250458at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050693">
        <property type="entry name" value="Hsp70_NEF-Inhibitors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013918">
        <property type="entry name" value="Nucleotide_exch_fac_Fes1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19316:SF18">
        <property type="entry name" value="HSP70-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19316">
        <property type="entry name" value="PROTEIN FOLDING REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08609">
        <property type="entry name" value="Fes1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nucleotide exchange factor Fes1" evidence="6">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="293" mass="32757" checksum="3D82187C60BBF05E" modified="2017-01-18" version="1">MEKLLQWSTAHENGDQEAIKRAGAPDPRILAQLFGKGGPDEPTLMKEAMIVLTNPEATLENRLIAFENFEMLIENLDNANNIENMKMWPPLLKFLTEESQEEDELVASALSVIGTAVQNNINSQEAFMKYDNSLKNVIKYAGDSSQPFDVRVKAFYALSNLLRHNPKSCHEFVKLGGLSIVAPVLTDPSVKSKLKLRALSAVSALLTSVGTDHDMVQTMRELGIVKASIISLSPEEKNIYVLDKVLNLLSHFITAGVKFTEEELSLLREHFXSIKPLKERLNEDDYLTVKHVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE87</accession>
    <name>A0A1E5RE87_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">COX2 translational activator protein, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1697</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE87">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE87"/>
    <dbReference type="InParanoid" id="A0A1E5RE87"/>
    <dbReference type="OrthoDB" id="4061518at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="799" mass="95076" checksum="7F7C88A89FE6EE9D" modified="2017-01-18" version="1">MKQYRYLKLLCIKRRYQCHPPIYQTHNCKEGGDVKSWLATFNQTDTNKDADXLKHDDSFSRPSPLQSKNASLMNNPSIPATPDTHCLVSTKEFEDFWVPKLKTPTILEYNLLHSELLRTNSLFTLEQIALLVKALFQYKKVFEIHQIYKKNHNRLLNELDTSTQLEENSITKMQILNVLMETEYLLTNYHNFEELFAILLKYRYPIKSKSIEMAFLSFINTSNFSMCKDFFQQVMSNTEQYQLNEKHFINIVKKLSLSGELELLRVFIETWVVNTYNNDYSLISKNLLRFIHSTTLEHEKNXYLSCSNMASIKKLSSMLKREYLKSVDAKSIKFYETLKRIQPKDIASLAQFQLLFEXFLEELQKKHASTVPVILLTVLSVASRNKQEGIFDWTLSNCIKNKQFFTYLDYVQLEQIITERLFLKHDLLGLITFIENARHMKLSNKSFKTLNETFLSRFPFLEKAVYPEYRILFSDPKFQETYPWCNIFLCKHHLEHSVDVIVKRKLTRQDASNDSKKENFFFDDLSTADKMKSSYTILKRYVNYRRGMKHIDETQIDKVIEFVRRVFEEGHFRYQNQLPLKFQMLQIEIEALRSFHKMSRLEKKILRKTVYKERIDNFVSSKSLLLRSDDYLRLANLVSKRPFKDFDYGKMFLNKSLENISTHTNNNAFELAKNWYKFYMTSLKISVQSHDITFFVEIVKDWNANKDAFYILPECVTDLKGFQKYWIKRKEFLMTSTQENSNFSDNLHVYDDFLADIDINVGQLKQRYVDYEYEGLNTMQKLVQSIKNRFEREVKAKNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE89</accession>
    <name>A0A1E5RE89_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Exocyst complex component EXO84</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2163</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EXO84 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE89">
        <property type="interactions" value="194"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE89"/>
    <dbReference type="InParanoid" id="A0A1E5RE89"/>
    <dbReference type="OrthoDB" id="642193at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030133">
        <property type="term" value="C:transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1220">
        <property type="entry name" value="Exo84p, C-terminal helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1210">
        <property type="entry name" value="Exo84p, N-terminal helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033961">
        <property type="entry name" value="Exo84"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032403">
        <property type="entry name" value="Exo84_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042561">
        <property type="entry name" value="Exo84_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042560">
        <property type="entry name" value="Exo84_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21426">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21426:SF12">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16528">
        <property type="entry name" value="Exo84_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25345">
        <property type="entry name" value="PH_EXO84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08700">
        <property type="entry name" value="VPS51_Exo84_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst component Exo84 C-terminal" evidence="9">
        <location>
            <begin position="584"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="279"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="484"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="222"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="484"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="839" mass="93852" checksum="29CF79AADE2498BF" modified="2017-01-18" version="1">MNTIKRVGTLSRSKSSLSAALDQNSEQKEANISKKNRRGKYEKSGYEHSLKNKQSTAELLIAKPYEEMVVPDISKEQRKKVGSSMQRRLSVHLPKDAAQMVPDFSNAPALPGNFSSDQYYLQSTNQGYATQSHGPDSTXILPKTTPQQQFQPKYSSAFLRKVLSDDRFVAREFVHDKLGDATAREIDNFTSSLNDLSLEVQDEIRLNMDQSYKEVIIVNKNLKLATQELKSLRGDIEDLNHTLKDFEVIASKKLESRAMLQNQDVPATPLLRTTQEGTMTPHSIASPNSLSKPTTGFGSTGLQDKSAKEKTSVLMLEKVWVSELNELWKKVEGAQKYVPAVPGRHIVFESADWFEMNAATLKPLSTACFIVLSDLMVIATKKHTVASVDKNVINSQATDPSAHNFVVAHCGPLKDVTISSNDKDLRLTVSFNMDASTSKSVFQCRDKKEFHKCISKIRLAKDSIKEDIYNEEQTTNKFRNSLINLQSSPVPNNVSPRKSSHKRNTSSGSANLYHNALSSNGFSNGATLTVKTDKRLSAAAPMSALTRTPGTPKKDDVSLLHTLSLSINARTKTRDIQSSAYQLKQIDHKVDSIDVFLSRKKFKEALKVVEDVEQNLNIYDQKFNVNSDTSKNMDEQDAMLRQIVGMKVQEKKSLLVEKLLSILQSNDYIVDISTIIDTMLCLIGCKSISESTILDAFLSNRSLYIAKLIANIKSSLLNTQIYALEVTVVRFQTLKKTVITFNELFQNSRIHNQLSSTLVSWTEAEIDDHFSTLTSIANMKNIVTPTFIKSTRKQIDLLKSVGMDFVYKLDKFLQQQRQKQITNTNFASNDTTGLGIVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE92</accession>
    <name>A0A1E5RE92_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fatty acid synthase subunit alpha</fullName>
            <ecNumber evidence="3">1.1.1.100</ecNumber>
            <ecNumber evidence="4">2.3.1.41</ecNumber>
            <ecNumber evidence="2">2.3.1.86</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="ORF">AWRI3579_g1859</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17397"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="1.1.1.100"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a fatty acyl-[ACP] + malonyl-[ACP] + H(+) = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.41"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>acetyl-CoA + n malonyl-CoA + 2n NADPH + 4n H(+) = a long-chain-acyl-CoA + n CoA + n CO2 + 2n NADP(+).</text>
            <dbReference type="EC" id="2.3.1.86"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. Fungal fatty acid synthetase subunit alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="29">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="3"/>
    <dbReference type="EC" id="2.3.1.41" evidence="4"/>
    <dbReference type="EC" id="2.3.1.86" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE92">
        <property type="interactions" value="367"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE92"/>
    <dbReference type="InParanoid" id="A0A1E5RE92"/>
    <dbReference type="OrthoDB" id="4251012at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005835">
        <property type="term" value="C:fatty acid synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004321">
        <property type="term" value="F:fatty-acyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042759">
        <property type="term" value="P:long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00828">
        <property type="entry name" value="elong_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08950">
        <property type="entry name" value="KR_fFAS_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.20:FF:000005">
        <property type="entry name" value="Fatty acid synthase alpha subunit FasA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2490:FF:000001">
        <property type="entry name" value="Fatty acid synthase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.25.70:FF:000001">
        <property type="entry name" value="Fatty acid synthase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="entry name" value="4'-phosphopantetheinyl transferase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040899">
        <property type="entry name" value="Fas_alpha_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047224">
        <property type="entry name" value="FAS_alpha_su_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026025">
        <property type="entry name" value="FAS_alpha_yeast"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041550">
        <property type="entry name" value="FASI_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050830">
        <property type="entry name" value="Fungal_FAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004568">
        <property type="entry name" value="Ppantetheine-prot_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00556">
        <property type="entry name" value="pantethn_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10982:SF21">
        <property type="entry name" value="FATTY ACID SYNTHASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10982">
        <property type="entry name" value="MALONYL COA-ACYL CARRIER PROTEIN TRANSACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18325">
        <property type="entry name" value="Fas_alpha_ACP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18314">
        <property type="entry name" value="FAS_I_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000454">
        <property type="entry name" value="FAS_yeast_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="4'-phosphopantetheinyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="26" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="17" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 24" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="15" id="KW-0520">NAD</keyword>
    <keyword evidence="13" id="KW-0521">NADP</keyword>
    <keyword evidence="14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 25" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carrier" evidence="27">
        <location>
            <begin position="149"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="28">
        <location>
            <begin position="1127"/>
            <end position="1666"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="26">
        <location>
            <begin position="360"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="active site" description="For beta-ketoacyl synthase activity" evidence="22">
        <location>
            <position position="1313"/>
        </location>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="1786"/>
            <end position="1788"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1786"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1787"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1788"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="1812"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="1822"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="1831"/>
            <end position="1847"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="1855"/>
            <end position="1858"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="1885"/>
            <end position="1887"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1886"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1887"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="25">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR000454-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR000454-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR000454-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR000454-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="EMBL" id="OEJ85217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1899" mass="209324" checksum="9E8EAD20B69F74B1" modified="2017-01-18" version="1">MPMKPEVEQELAHVLLTELLAYQFASPVRWIETQDVFLKDLKTERVVEIGPSPTLSGMAQRTIKNKYESYDAALSLQRQVLCYSKDAKEIYYTPDPSELEAAAAPKEEEAAAVPASAPAAAPAAAXAAPAPVAAAAPVAAAAELPDSPVGADLILHVLVAQKLKKSLDQIPMSKAIKDLVGGKSTVQNEILGDLGKEFGSTPEKPEEIPLSELAETMQETFNGQLGKQTLSLISRLMASKMPGGFTVTVAKKYLQSRWGLQSGRQDGVMLVALTSEPASRLGGEADAKQFWDSMAQKYASIAGVDLSAASSAAAGAGAGAGAGAGGASIDAAAFDELTKDQKVLARQQLECLARYLKIDLNKSERKFLKEKDNLEGLQQQLDFLNEELGEFFINGVQTKFSRKKARVFDSSWNWARQSLLSMYFQIIHGVLKNVDRELVSEAINIMNRSNETLIKFMEYHISHTNEEKGETYRLAKTLGEQLIANCKEVLNVAPVFKDISKPTGPHTTVDKNGNIEYTETPRQHAIKFSEYVQEMALGGPATQQADEPTIQDDLTNVYHAINSQVKEHGISAKTQLEFETLYGELMKFLKASNEIDTKVSTKDLQGETKNNHLDSDSTKEVASLQSATSIETKVSSKIPKKTIPFLHLKKMIKGGEFVYDRAITGSFLDGLEKAAVNGLTFQDKYVLMTGCGIGSIGASMLQGLLQGGAKVVVTTNSFNKKTTEHFQALYSKWGAKGSTMVLVPFNQGSKQDVQDLIKYIYDDEKAGGLGWDLDAIIPFAAIPEQGIELENIDSKSEFAHRIMLTNIYRIMGNVKKQKSDRGIETRPAQVILPLSPNHGTFGGDGLYSESKLSLETLFNRWISESWGNQLTVCGAVIGWTRGTGLMSANNIIAEGIEKMGVRTFSQKEMAFNLLGLMTPEVVEMCQTSPVMADLNGGLQFLDDLKTFTSQLRTDLYKTSDIRKAVSIETSMEHKVVSGSTNADAGYSKVEVQPRANLDLQFPQLKKYEQIKQLSPDLEGMLDLERVIVVTGFSEVSPWGSARTRWEMEAKGEFSLEGCVEMAWMMGLIKYHSGNLKGRPYTGWVDAKTNQPVDDKDVKNKYEAHILDHSGIRLIEPELFDGYDPKKKHMIQEVVIEEDLEPFETSKETAEQFKHEHGDKADIFEIPESGEYTVKLLKGATLYIPKALKFDRLVAGQIPTGWDAKTYGISEDTISQVDPITLFVLVSVAEAFITSGITDPYEMYKYVHVSEVGNCSGSGMGGVSALRGMFKDRYKDSPVQNDILQESFINTMSAWVNMLMISSSGPIKTPVGACATAVESLDIGVETILSGKAKICIVGGYDDFREEGSYEFGNMKATSNSLEEFEHGRTPSEMSRPATTTRNGFMEAQGAGIQVIMNAELALKMGVPIYGILGFTATATDKIGRSVPAPGKGILTTAREHRGSLKHEPKILDISYRKRQLKLRSQQIKIWEESELELLKDESMDIEDLKLRREYVEERSIEISKEALQQLKSAQNQWGNEFYKKDSRIAPLRGSLATFGLTIDDLGVASFHGTSTKANDKNESATINEMMKHLGRSEGNPVLGVFQKYLTGHPKGAAGAWMMNGALQILNTGIVPGNRNADNVDKILEQFDYVLYPSQSLQTDGVKAVSITSFGFGQKGGQAIAINADYLYCCLTEEQYYEYKSKVALRERQANQFFHKGMINNTIFQSKEHAPYSDELEQQIYLDPLVRVSKQLEKNEDGFYKFTKKDVQNSERFFFDQSTKKTGDVLNELVSQVKTESSNVGVDVELIREVPIANTNFLERNFHKSEIEYCSKQPSAQSSFAGLWSAKEAVFKSLGIKSQGGGASLIDIVIKRENGNAPIVELKNELNEIAKKKGISNVKVSISHDDFQSVAVAIAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REA0</accession>
    <name>A0A1E5REA0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutamate-5-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="2">1.2.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Glutamate-5-semialdehyde dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Glutamyl-gamma-semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g2231</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19541"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:58274"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the gamma-glutamyl phosphate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.41" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REA0">
        <property type="interactions" value="907"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REA0"/>
    <dbReference type="InParanoid" id="A0A1E5REA0"/>
    <dbReference type="OrthoDB" id="1934954at2759"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00360"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004350">
        <property type="term" value="F:glutamate-5-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07079">
        <property type="entry name" value="ALDH_F18-19_ProA-GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000006">
        <property type="entry name" value="Gamma-glutamyl phosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00412">
        <property type="entry name" value="ProA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020593">
        <property type="entry name" value="G-glutamylP_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012134">
        <property type="entry name" value="Glu-5-SA_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000965">
        <property type="entry name" value="GPR_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001221">
        <property type="entry name" value="PRK00197.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00407">
        <property type="entry name" value="proA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11063:SF8">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11063">
        <property type="entry name" value="GLUTAMATE SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000151">
        <property type="entry name" value="GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01223">
        <property type="entry name" value="PROA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="12">
        <location>
            <begin position="4"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="13">
        <location>
            <position position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00077451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ85196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="447" mass="48975" checksum="7492EC6CCF931CC5" modified="2017-01-18" version="1">MSAAEQIAKSARKAGNVLKTVDDEHRSLVLQKIHDALKANKDLIKAANEQDLKVAAENNLASSLVKRLDLFKSDKFETMLQGILDVANLEDPVGKVLMARELDENLTLYKVTAPVGVMLVIFESRPEVIANITALAIKSGNAAILKGGKESLETFREMSKIINTCVQENFATTGVPVGSVQLIETRQDVSDLLNQDDYIDLVVPRGSNALVKNIKNNTKIPVLGHADGICSIYCDSEADLEKAKNITVDAKTNYPAGCNAMETLLINPKLHNWWDILDNLVANNVILHVTKDVKAEYFKHASNESQKKVVDVDESKDFDGEFLSFDCAVKFVYNVSEAIEHINAHSSKHTDSIITENKETANKFLKGIDSAGVYWNCSTRFADGFRYGFGTEVGISTSKIHARGPVGLDGLVTYQYQMIGNGQIAGDYLGAGGQRAFVHKDIDIXSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REA1</accession>
    <name>A0A1E5REA1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="20">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">AWRI3579_g2232</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 20">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38567"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:38568"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Membrane</location>
            <topology evidence="20">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="20"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REA1">
        <property type="interactions" value="306"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REA1"/>
    <dbReference type="InParanoid" id="A0A1E5REA1"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140346">
        <property type="term" value="F:phosphatidylserine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090556">
        <property type="term" value="F:phosphatidylserine floppase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006892">
        <property type="term" value="P:post-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000026">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000087">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000010">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF150">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 18 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 19 20" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 18 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 20" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="408"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="450"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1009"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1050"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1098"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1122"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1165"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="22">
        <location>
            <begin position="151"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="23">
        <location>
            <begin position="942"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="17">
        <location>
            <position position="522"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="522"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="522"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="523"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="524"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="524"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="617"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="660"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="683"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="717"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="797"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="798"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <feature type="binding site" evidence="18">
        <location>
            <position position="799"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
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        <location>
            <position position="890"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="896"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <position position="916"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
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        <location>
            <position position="919"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <position position="920"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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        <location>
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        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="OEJ85220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1277" mass="145556" checksum="EA14F29A51E21B11" modified="2017-01-18" version="1">MSAKQKDNSLFDLDFLENDADKGETNASPSSNREFPMETLDLDDISIENDYGDPFADRNTYPQTGSSQVGDEPLHKAQKNGFFKRLFGKKTAVTASDNAYEMHTYSNIGSAANDSFLNSTKQYDIKALFEKYLLGRGDGENISLEPRTILINDAHSNSHFKDNXVSTTKYNFATFLPKFLFQEFSKYANLFFLFTAIIQQVPDVTPTNRYTTIGTLTVVLFISAIKECVEDIKRANSDKELNYSPVEVFDPMLKGFTLKKWIDIKVGDIIKLTSEEAVPSDIVLISSSEPEGLCYVETANLDGETNLKIKQARPETSKFFDVQQLAQMSGQLVSEAPNSSLYTYEATMTLNGKSFPMSPEQLVLRGATVRNTSWVYGFVVNTGHETKLMRNATATPIKRTKVERIINVQIIMLFGLLILFSFISSLGNIIQMTAQSKNLEYLYLDRVNKVGLFFKNILTFWILYSNLVPISMFVTLEMIKYYQAWMISSDLELYSEETDTPTIVRTSSLVEELGQIKYIFSDKTGTLTRNVMEFKSCSIAGKCYIEKVPQDKKATMEDGVEVGYRSFEDMFKKLADTFDDDSAVINQFLTLLSICHTVIPEFRDDGTIKYQASSPDEGALVEGAAKLGYKFLIRKPTSVRILVESSGEELEYKLLNVCEFNSTRKRMSAIFRFPDGSIKILCKGADTVILERLGEDGMEHVEATTRQLEEYASEGLRTLCIAYRDISEEEYQMWNTAYQEAATTLDNRGEKMDAQAEIIEQNLILLGATAIEDKLQEGVPETIQTLQEAGIKIWVLTGDKQETAINIGMSCRLLSEDMNLLIVNEETKEETRKNFIEKFQAFKEHNISSSDLENLALVIDGKSLSFALEPDLEDYLMQLGKMCKAVICCRVSPLQKALVVKMAKRKTKDILLAIGDGANDVSMIQAAHVGIGISGMEGMQAARSADFAIAQFHYLKRLLLVHGSWSYQRISQAILYSFYKNIALYMTQFWYVFANVFSGQSIMESWIMTYYNVFFTVLPPFVLGVFDQFVSSRLLERYPQLYTLGQKGQFFNVTIFWGWIINGFYHSAITFICSILFYKYGNSLNMHGETVDHWTWGVAVYTSSIITVLGKAALITNQWTKFTFFAIPGSLLFWLVFYPIYAAILPHWDISYELRGTISHCYRSATFWLMCIVVPMFSLLRDILWKYYKRNYNPATYHIVQEMQKYKITDNRPRVEQFQKAIRRVRQVQRMKKQRGFAFSQSEEGGQEKIMRMYDTTQKRGEHGELLDASANPFRDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REB1</accession>
    <name>A0A1E5REB1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Altered inheritance of mitochondria protein 39, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1699</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REB1">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5REB1"/>
    <dbReference type="OrthoDB" id="4058511at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="389" mass="45640" checksum="25E0B8911940FF6A" modified="2017-01-18" version="1">MTGNIRLSNVLXXSSQETXSKIFSVRHISISHVTKTPNQNKSXPNSLNHFQKAATKSNDKKLDQNPNLNFIQQQEDFLQKNHPATSSSQKNATSNXLPPQLQPSVMNNAQDLSRLQSSIIQQQKLKQRKTAVYSFFIILLGSFFGYTIFYKVLYLNEESFWPLFPVQSTRELTPKEMDQLDIESMYELAQQQALFKLQLNDMVKKRYTIPLXISQKXGPHAKNFDKIWFQDSSPDTFQFKILRFKPYDVSNYKKGYHSLGHWIQFKIETQRFMSLKKLWNNILGNEEVYQLIHERSQINGEKSLEEQNEEMMMYRYDPSVKESEPEIWFLNEFKLLEDNSSIIYKGKCVHNCDVSISQIDLVTKNVTDGKMVRYVLYKENETYPKDRDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REB6</accession>
    <name>A0A1E5REB6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNA polymerase I-specific transcription initiation factor RRN11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REB6"/>
    <dbReference type="FunCoup" id="A0A1E5REB6">
        <property type="interactions" value="83"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REB6"/>
    <dbReference type="InParanoid" id="A0A1E5REB6"/>
    <dbReference type="OrthoDB" id="2159786at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070860">
        <property type="term" value="C:RNA polymerase I core factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001164">
        <property type="term" value="F:RNA polymerase I core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001181">
        <property type="term" value="F:RNA polymerase I general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053029">
        <property type="entry name" value="RNA_pol_I-specific_init_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007224">
        <property type="entry name" value="TIF_Rrn11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28244">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28244:SF1">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04090">
        <property type="entry name" value="Rrn11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="490"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="566"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="513"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="575"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="587" mass="68520" checksum="8ECF5AD654E386A2" modified="2017-01-18" version="1">MVFEVPLNVNNKKIRKTKKIRYKYKFFLNKYHQENEAKKWRANVEIGKELATPSNDDRRTSVLTPQPSEFETEHENSESNHEHKKKIDYETIVLSKYHKPEDQYEQWYPSSTSFQKQRQAHFPMLKNNATINESIPCVHASYVEKLKKQACSGMTNSELQWLAKDIQKHETCDDIEDNALQTSSTLRNQMAPMEHTYMGKHVYNIIQIWHFAMLDHKWDKAYRCFSILLRIPNVDIRSIWSLGCATLKNHQHAQILEAEKRSGSFDSNITSQNNANNIGVFSFNPQFSVSDTFNKKNVNANQLSYMKFLEWMCTVFSSRTHFNQNLNYMMDPVWRKGSYKHTPVYVYTWLWSLLVESSTLNLENLQSQQTTQNGFSQNSNDDMGDDVVQWIIEKINDMILTPPYMDDPMIKYIQACAHLVKADELVSGPLSTRRRNTAIIGQHVLSCKKLLRMISSSQVNASIDKKFCFNKRFIEKQLQIIEKHLYIEESEDESEDETRQTIVYSDPPLPKKIHTEETNSLHGGLDDLRSGFEEESLDIETQTQQGDDFADDSIAYDSDVYARLENDDEDNQEIDTFHNDHHYSSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REG2</accession>
    <name>A0A1E5REG2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">54S ribosomal protein L50, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1716</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REG2"/>
    <dbReference type="FunCoup" id="A0A1E5REG2">
        <property type="interactions" value="125"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REG2"/>
    <dbReference type="InParanoid" id="A0A1E5REG2"/>
    <dbReference type="OrthoDB" id="5555409at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.10">
        <property type="entry name" value="Ribosomal protein L9, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036935">
        <property type="entry name" value="Ribosomal_bL9_N_sf"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="147" mass="16903" checksum="307CC6D443C54012" modified="2017-01-18" version="1">MRSSSVLLSALTKRTKKVRVQILKDFPKLEYLKGDVVEVKPSLMNNVLHGHNGAKFILQSDDIDQALLAKATSLRTARQEMLERERQSQVQLVEQQLKKQKEVKFFSENKKDEQNESKKDDENKKLENSAIDENLTLKDIKIPGLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REJ8</accession>
    <name>A0A1E5REJ8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein LST7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REJ8">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REJ8"/>
    <dbReference type="InParanoid" id="A0A1E5REJ8"/>
    <dbReference type="OrthoDB" id="5599713at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037521">
        <property type="entry name" value="FLCN/SMCR8_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021713">
        <property type="entry name" value="Folliculin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037520">
        <property type="entry name" value="Folliculin/SMCR8_longin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31441:SF2">
        <property type="entry name" value="FOLLICULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31441">
        <property type="entry name" value="FOLLICULIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11704">
        <property type="entry name" value="Folliculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51834">
        <property type="entry name" value="DENN_FLCN_SMCR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN FLCN/SMCR8-type" evidence="1">
        <location>
            <begin position="90"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="277" mass="32322" checksum="405A36C3D83B1FCE" modified="2017-01-18" version="1">MSSSSSPSCAKRNHQKNNNXKATFHMSASIESHFFITLAHFCDIHGPRVIQVTQVARSAESLEQLLLRDYPTDAYCDSCRFQFPNVDKQKEKQHIAQDVDIKLRSMESHIDSAHYVSTQYSATRYLLLSSIIKKVFSEETMVYDESPLLFYDEERGFNVAVGFKLQDNYSRGNERRYCFIFSISDVNEVLCMNVVQRHLEFLLSSFGRMITFVKTQRSEVLLQLQQDQQNPFMPTSSYLRSNTMKLPRSLSRLVQDEQFFVKLHKWNAYILRNIDLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REL7</accession>
    <name>A0A1E5REL7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Stress protein DDR48</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1873</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REL7"/>
    <dbReference type="STRING" id="56408.A0A1E5REL7"/>
    <dbReference type="InParanoid" id="A0A1E5REL7"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="183"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="229"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="426" mass="45307" checksum="603917E934DFA4AF" modified="2017-01-18" version="1">MGLFNKAKEFAQNNLNNINNNDDSYGSGNNNDSYGNNNNNDSYGNNNNNNNNNNDSYGSNNNNSYGSSNNNDSYGSNNNDSYGSSNNDDSYGSSKKNSNNNDSYGSNNNDSYGSNNNDSYGSSNNDDSYGSSKKDSYGSSNNDSYGSNNNDSYGSSNNDSYGSSNNDDSYGSSKKNSSKKDSYGSNNNDSYGSSNNDSYGSNNNDSYGSSNNDDSYGSSKKNSSKKDSYGSSNNDSYGSNNNDSYGSSNNDDSYGSSKKDSYGSSNNDSYGSSNNDSYGSNNNGSYGSNNNNDSYGSNNNDSYGSSNNNDSYGSNNNDSYGSSNNDDSYGSSKKNSNNNDSYGSNNNDSYGSNNNDSYGSSNNNDSYGSSKKDSYGSSNNDSYGSSNNDSYGSNNNDSYGSNNNDSYGSNNNNDSYGSNNNNDSNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REM3</accession>
    <name>A0A1E5REM3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent DNA helicase II subunit 1</fullName>
            <ecNumber evidence="4">3.6.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">ATP-dependent DNA helicase II subunit Ku70</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AWRI3579_g1677</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ku70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REM3">
        <property type="interactions" value="691"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REM3"/>
    <dbReference type="InParanoid" id="A0A1E5REM3"/>
    <dbReference type="OrthoDB" id="3249161at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043564">
        <property type="term" value="C:Ku70:Ku80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006164">
        <property type="entry name" value="DNA_bd_Ku70/Ku80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006165">
        <property type="entry name" value="Ku70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005160">
        <property type="entry name" value="Ku_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005161">
        <property type="entry name" value="Ku_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016194">
        <property type="entry name" value="SPOC-like_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604">
        <property type="entry name" value="KU AUTOANTIGEN DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604:SF2">
        <property type="entry name" value="X-RAY REPAIR CROSS-COMPLEMENTING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02735">
        <property type="entry name" value="Ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03730">
        <property type="entry name" value="Ku_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03731">
        <property type="entry name" value="Ku_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003033">
        <property type="entry name" value="Ku70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100939">
        <property type="entry name" value="SPOC domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 20" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="VWFA" evidence="19">
        <location>
            <begin position="49"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with DNA; for 5'-deoxyribose-5-phosphate lyase activity" evidence="18">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR003033-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OEJ85347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="617" mass="71296" checksum="10D7CFAB2B27E76E" modified="2017-01-18" version="1">MADEFDFSXYIKGIKRNDPDYYLDLTESNEYYNDDYDPKDKKKFRVHEGIIFLVEVTNSLLSPYETARGEVGTPPILDIFDSLNATIEQLTITLPNTGIGFYIFNSSNHDPDSAMKMIFPLRDINIEDMKLVNEYMEDISSSKLDLIKEWDPINQSSDTSDGNEADNGKTSSSSLIKVLSEIQFKFKKENLIENREFNKRKVFFFTDNDNPKNIETARCRIVLNDLKTNDIEFNPFLISAGKNVFDTSKFYDEIAKKLSHGVVLNVQSIKPNLLKEKVLRTKEIERKLFFCPLFLNESISIGVLGFLTFQQENPVARSKMYHAIEKTDAFSKRQYLSESTGEDITKSITPCFELMNKKNAKVTDGKKFEVTNDPSSMDPFLKIIGFAKNKDIFSYFTNVEKPYFIIPDETELKGSTKAFAALYKSLFKQQKSCLLIGKIRKANTIQNYLMTINGTDGTGFYLYRVPSKEEIRLFPPINEELCETLENGDTDKREDYKKMYDVASFLLKRVTMKNAYNPKDYRNPQVQKFYRVLRDFLLQVEVPEDDENIDSQSDVITRNADLDDTLEGLKSTFNVLQANKECQEKLKEWLSIYKRNGIESMTPSAPTIQKRYTKXKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REU4</accession>
    <name>A0A1E5REU4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">uridine/cytidine kinase</fullName>
            <ecNumber evidence="2">2.7.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1731</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.48" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REU4"/>
    <dbReference type="STRING" id="56408.A0A1E5REU4"/>
    <dbReference type="InParanoid" id="A0A1E5REU4"/>
    <dbReference type="OrthoDB" id="738517at2759"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00637"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004849">
        <property type="term" value="F:uridine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02023">
        <property type="entry name" value="UMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000764">
        <property type="entry name" value="Uridine_kinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004018">
        <property type="entry name" value="PRK05480.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00988">
        <property type="entry name" value="URIDINKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="6">
        <location>
            <begin position="6"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="221" mass="24479" checksum="C42643F76098ED83" modified="2017-01-18" version="1">MIAPYIVGIGGPSGSGKSSIAKYIISELGQENVALLPMDNFYKPLNEIECQQAQNEELNFDTPDAVDLAHIRACLKELKAGHSVEIPVYSFASYNRIPNVTHTVCSAKIVVLDGLYALSDPGILDLLDLKVYVDVDLDVCLARRLLRDIVHRGKNIDTCMHQWITRTRCDLEKNVTPTLHRADIVIPNCDHSKDATIQMVVGHLKNVLNTEAKAEHVSLSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF48</accession>
    <name>A0A1E5RF48_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SERF-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1800</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF48"/>
    <dbReference type="FunCoup" id="A0A1E5RF48">
        <property type="interactions" value="227"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RF48"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007513">
        <property type="entry name" value="SERF-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04419">
        <property type="entry name" value="SERF-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small EDRK-rich factor-like N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="66" mass="7610" checksum="A9A372ED20174A58" modified="2017-01-18" version="1">MARGNQRDLARAKNLKKQQEASKSQKKDGSKQKRMESDADILRQKQAAADARKAAEELERKKKEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF90</accession>
    <name>A0A1E5RF90_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">SWI5-dependent HO expression protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SHE3</name>
        <name evidence="13" type="ORF">AWRI3579_g2289</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">RNA-binding protein that binds specific mRNAs including the ASH1 mRNA, coding for a repressor of the HO endonuclease. Part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud and in the daughter cell. Required for the delivery of cortical endoplasmic reticulum into the emerging bud.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the SHE3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RF90">
        <property type="interactions" value="1401"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF90"/>
    <dbReference type="InParanoid" id="A0A1E5RF90"/>
    <dbReference type="OrthoDB" id="6088208at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048309">
        <property type="term" value="P:endoplasmic reticulum inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031398">
        <property type="entry name" value="She3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17078">
        <property type="entry name" value="SHE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="373"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="434"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="136"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="11"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="379"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="435"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="471"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="509"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="531"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="560"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ85569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="571" mass="64285" checksum="D7D8ED7E14CAF41B" modified="2017-01-18" version="1">MDSPTPPPKINDTSLQDLRSQDSPSRTQPRFDIQHGTFIANLEKQSPTRQRYKSTLAEPMDGTSFEDSFTSSQSKSSKVIESLHQKIDTLTNTNLQLTYQSNNLLTRLEEANLKQSKHIDTINKLKLENGNLSAMYERKSRKAKEMETQLEEINIKLDKTCFENENLNKQMSNDTANKQSLEEQLQMSHMQYQALQDAQARYKQNYESQIEEMQAQIFEIKMSYKKILSDKFQALSNLDSKWEQSFGQLSSQVNSINSINKIEISSLNKEMEDLKIDYINNENLSNLYSKSKEMAFEYAELIDMNLPDAFKQKHNLVDGSFTLELKKMEALELQQKQRSLAIKNTENMLTSTPTKTATNITTFGHTKASENFTKNIPLXNNSGNVQNSSNANRKSKRNSSFYGSLGTYVADSDTSDNSSILLDDQTLHHDEQHNGKLSFSNASSAQHSNPSNGQLNFNNMSTKEKRASWLASVSNTGNTVPSANNRSISPFQNSAARFPSNSRGGSRHSSLNNNPSTNSNPNKTSGLPGLTKNSSGTRKTSSSFEDKNDATFSENPNPNKLKRAGSVKRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF94</accession>
    <name>A0A1E5RF94_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Atypical kinase COQ8, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1758</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF94"/>
    <dbReference type="FunCoup" id="A0A1E5RF94">
        <property type="interactions" value="404"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF94"/>
    <dbReference type="InParanoid" id="A0A1E5RF94"/>
    <dbReference type="OrthoDB" id="201153at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13970">
        <property type="entry name" value="ABC1_ADCK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034646">
        <property type="entry name" value="ADCK3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051409">
        <property type="entry name" value="Atypical_kinase_ADCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43851">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43851:SF3">
        <property type="entry name" value="COENZYME Q8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="6">
        <location>
            <begin position="206"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="68"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="75"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="551" mass="63048" checksum="B397641A5B4413F5" modified="2017-01-18" version="1">MASRQGLYDLYCVLSSSKEVLGNTFNLGKQSLQSWSHTSMIARPILSRYQWFYDPKWEEAKKLAQEVKEDGNLKTKGHTTSKKKHAKRDLSTGTTTSKRHFSTISMRLNEGKKDPESVQKEQAARVLEMIDVYISNVGFSAARQGLNKMSKGEKPTMKSLLLSNENIDRIVKKFSKMRGAALKIGQMLSFQDEKVLPKELYEILSRVQNNANYMPLKQLDKLMQKEFHTAEWDTKKFSKFDKIPIAAASIGQVHEATLKDNTEVVVKIQYPGVKDSIDSDLNNLLLLLTASSLLPKGLFLDKTVANARTELKWECDYIREANCLKKFEKLLQKDPVFAVPHVYDDYTTSNVLTMTKMKGVEIMKLEHASQEMRDFICTNIMRLCLQEIAEYQYMQTDPNWANFLYNKDTQKIELIDFGATRPYPPQFIKNYRRLLTYAAVKHNRKECAAVSKDLGYLTGLESSKMVDAHVDSVLVLGEPFSQPGLFDFGNQTVTDRIRGNIGVMLNERLVPPPEETYSLHRKFSGVFLLCARMKARVPCHDLFKKHFAIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFA1</accession>
    <name>A0A1E5RFA1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g1759</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family. DEAH subfamily. DDX15/PRP43 sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFA1"/>
    <dbReference type="FunCoup" id="A0A1E5RFA1">
        <property type="interactions" value="1553"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFA1"/>
    <dbReference type="InParanoid" id="A0A1E5RFA1"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022613">
        <property type="term" value="P:ribonucleoprotein complex biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17973">
        <property type="entry name" value="DEXHc_DHX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000007">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000324">
        <property type="entry name" value="pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000003">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044756">
        <property type="entry name" value="DHX15_DEXHc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF109">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 16" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="103"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="293"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="18"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ85546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="767" mass="87212" checksum="A267B13A342577E8" modified="2017-01-18" version="1">MGSKRKFEDNASVADGITSDSSVESFSSMTNKTDLEVNEEPLVHHDMGEFKGMVEHQTTAKQAEALEGGKVNPFTGENYSDRYFGILKVRKDLPVHSQRQEFLDIYQKSQIMVFVGETGSGKTTQIPQFVLFDEMPHLKNTQVACTQPRRVAAMSVAERVAQEMDVKLGEEVGYSIRFENKTTNKTILKYMTDGMLLREAMEDHDLKRYSCIILDEAHERTLATDILMGLLKQVIERRPDLKIIIMSATLDAEKFQKYFNDAPLLAVPGRTHPVEIYYTPEYQKDYLDSAVRTVLQIHATEGEGDILLFLTGEDEIEDAVAKISLEGDRLVREEGCGPVKVYPLYGSLPPHQQQKIFEKAPEGFNGRPGRKVIVSTNIAETSLTIDGIVYVVDPGFSKQKVYNPRIRVESLLVTPISKASSQQRAGRAGRTRPGKCFRLYTEDAFKKELIEQSYPEILRSNLSSTVLELKKLGIDDLVHFDFMDPPAPETMMRALEELNYLKCLDDEGNLTALGRLASIYPLDPMLAVMLIRSCNFKCAPEILTIVSMLSVPNVFVRPGKDKKRADDIKAEFSHPDGDHITLLNVYHGFKSDQAYEQGVRKWCFDNFLNHRSLLAADNIRSQLERMMVRHNLDLTSLDYEDPSYFDNIRKALTQGFFMQVAKKRSNGKGYLTVKDNQSVLVHPSSVLGREAEWVIYNEFVLTTQNYIRTVTSIKPEWLFEFAPAYFQLDHFQKGDVKLSLERVKERFDVYAKLDEGKKKKKAKKSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFA3</accession>
    <name>A0A1E5RFA3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutathione peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2048</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the glutathione peroxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFA3"/>
    <dbReference type="FunCoup" id="A0A1E5RFA3">
        <property type="interactions" value="562"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFA3"/>
    <dbReference type="InParanoid" id="A0A1E5RFA3"/>
    <dbReference type="OrthoDB" id="446890at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047066">
        <property type="term" value="F:phospholipid-hydroperoxide glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140824">
        <property type="term" value="F:thioredoxin-dependent peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00340">
        <property type="entry name" value="GSH_Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000010">
        <property type="entry name" value="Glutathione peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000889">
        <property type="entry name" value="Glutathione_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029760">
        <property type="entry name" value="GPX_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592:SF78">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00255">
        <property type="entry name" value="GSHPx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000303">
        <property type="entry name" value="Glutathion_perox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01011">
        <property type="entry name" value="GLUTPROXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00763">
        <property type="entry name" value="GLUTATHIONE_PEROXID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51355">
        <property type="entry name" value="GLUTATHIONE_PEROXID_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 8" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="9">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000303-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="162" mass="18353" checksum="FC6261ACFAC57F3A" modified="2017-01-18" version="1">MSKFYELSAIDNAGHDFSFKQLEGKVVLIFNSASKCGFTGQLDGLEKLYEKYQDKGLVIIGFPCNQFGNQEPGDDTQIQSFCSMNYGVKFQMMKKIDVNGSNESPVYHFLKQQKSGLLGFKGIKWNFEKFLINRKGEVVSRHSSMTKPESLEKEIAALLNEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFF8</accession>
    <name>A0A1E5RFF8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histone deacetylase</fullName>
            <ecNumber evidence="3">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2180</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase family. HD type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFF8">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFF8"/>
    <dbReference type="InParanoid" id="A0A1E5RFF8"/>
    <dbReference type="OrthoDB" id="73273at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF14">
        <property type="entry name" value="HISTONE DEACETYLASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="10">
        <location>
            <begin position="213"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="11">
        <location>
            <position position="7"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="519" mass="59614" checksum="215D34F1FD6389FA" modified="2017-01-18" version="1">MEKEEIDKTQVKRKHVDNDQNQFHKLPKLSNVVXVPGVQAEDDIYLTISMSIVQSMIADLLPCNKSFKSLLSTFLILKLPVKHGSSRTTILDNFDDIVTLPWCNPRDLKTYHASSYVDILLDXNFNDVENSSLFEHLRPYLKELQNAHDYITGLNLNTEELDGDLTDSDSSEPRFNISNEARGLCEYLKHEHFYDFESGDKVLDFDTPFSNETRRSNNDVLEPHVKDFIEKDXHKMIHGYKKHILLKHNLFYDCPIFPFLPLYCHIIPGATLKVVENLINNGRPNGIGINLDGGRHHAFKDKGNGFCYINDIVLAINRFRLKNYKKIAYIDFDLHHGDGVEKAFETSSNVQTTSLHFAEYGFFPNTAQKTLFDKQKECYNIVMPRGTNDAQFMDTVSNVVLPTVHRFCPQVLVIQCGGDGLKKDDYDVWALSIQGLVNAIMRLVEQFGKHTKIVVLGGGGYRISLQSRFYAYLIYKLTAYSKSGNSSKNNLLDKDYTFEIIPEHSFSDFYRDSYQKFFX</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGP4</accession>
    <name>A0A1E5RGP4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase</fullName>
            <ecNumber evidence="4 14">2.4.1.256</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g1094</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the third and last glucose residue from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide intermediate Glc(2)Man(9)GlcNAc(2)-PP-Dol to produce Glc(3)Man(9)GlcNAc(2)-PP-Dol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>an alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate = a alpha-D-Glc-(1-&gt;2)-alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29543"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19502"/>
            <dbReference type="Rhea" id="RHEA-COMP:19512"/>
            <dbReference type="Rhea" id="RHEA-COMP:19522"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57525"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:132522"/>
            <dbReference type="ChEBI" id="CHEBI:132523"/>
            <dbReference type="EC" id="2.4.1.256"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:29544"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the ALG10 glucosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.256" evidence="4 14"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGP4">
        <property type="interactions" value="686"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGP4"/>
    <dbReference type="InParanoid" id="A0A1E5RGP4"/>
    <dbReference type="OrthoDB" id="4769at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106073">
        <property type="term" value="F:dolichyl pyrophosphate Glc2Man9GlcNAc2 alpha-1,2-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016900">
        <property type="entry name" value="Alg10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12989">
        <property type="entry name" value="ALPHA-1,2-GLUCOSYLTRANSFERASE ALG10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12989:SF10">
        <property type="entry name" value="DOL-P-GLC:GLC(2)MAN(9)GLCNAC(2)-PP-DOL ALPHA-1,2-GLUCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04922">
        <property type="entry name" value="DIE2_ALG10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028810">
        <property type="entry name" value="Alpha1_2_glucosyltferase_Alg10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="197"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="295"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="333"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="420"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="445"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="479"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR028810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ86065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="512" mass="61046" checksum="D46E7674284CB729" modified="2017-01-18" version="1">MASTEDHGEQNLADTNILSGDDEVLQEITKQVEQSLILNIVVFYPLLVIYFIIAFYLXXKHVPYLFIDEIFHVPQTLRYLQGEWFSWHPKITTPPGLYILGWMNYKILKYIPLWKKCSTLTLLRTTNWFGGVIVWPVFVLRPMFYFQALGFWPTSLMAFPLLSTYYTLYYTDVWSTIFIVQALTYVITLPFGETKSIWLSALCVLISVIFRQTNIIWSLFILVLVVERRALIEQNFNTLWYNNYLKTLIHALENFYKLVLPYALNFVLFCIFLVYNRSITLGDKTNHVAGVHLVQVFYCYMFMAFFSMPLWFSKTHLVNYKTRFFSLKTFGQLILIIFIIRFFTKVHPFLLADNRHYTFYIYKKILNRNKLFKYVLCPIXYHFSRYTVHELMRMSLLPLGPMQGIKVKNTLDVPSQLTHVSWNVFYFCMVLTLVPSPLFEPRYYILPYIFWRVFVIAPYDSFTAYGVDKKSNLHRLKLELLWMVLINVVTLFIFLKFKFSWTTEAFPQRIIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGQ5</accession>
    <name>A0A1E5RGQ5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Membrane-anchored lipid-binding protein SIP3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1564</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGQ5"/>
    <dbReference type="FunCoup" id="A0A1E5RGQ5">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGQ5"/>
    <dbReference type="InParanoid" id="A0A1E5RGQ5"/>
    <dbReference type="OrthoDB" id="10070851at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd13280">
        <property type="entry name" value="PH_SIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042067">
        <property type="entry name" value="Sip3_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14248">
        <property type="entry name" value="CYCLIN Y, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16016">
        <property type="entry name" value="VASt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1044"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1071"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="6">
        <location>
            <begin position="352"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="7">
        <location>
            <begin position="808"/>
            <end position="1004"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1249" mass="144568" checksum="D424AFC826004911" modified="2017-01-18" version="1">MSNNPVPEKHENSVEAKKTIPLKDHVHILKLICVGFKESPLDSPSFRASVNYFNQQLETIEHWIEQVIKTKKFKAKDQFEEFQKFQNQLCEILLPPVNFLNSGVLDGTECTPEVLTRFHTTLKQVMEKVFSILKGNPEEYSAQVQLLLEKVVKPYKEVRKNFEYYQVKYDSFLDKYQSAKYHGVDPSTMREDAFQLFEVRKKYLQYSLELVLQIAGTNNRLSEHLVDLLCSLKSENEVFTDDLGSTVVIFTDVLSNFTAAKHWFHTSIAAHKSLIQDIYRARDQVEKSYIAEITPSRNIDHYNLHTVIEKLEEAKKLVKEQVADESSETLLAQGKKQRSAQPISTSGQNFSLHEKSGWLYMKTTIGVPGRTVWVRRWCFIKNSVFGMLALAPGKNFVQETDKFGVLLTKLKYNHKEDRKFCFELQISGSKDDINIVLQADSYIEMEEWFKCVSYVKEKLVSKNISKDEYELAFLRFPPLYTEYACSSSTSIDNLITTSENCVSLVEKCGAKWTPYGEIIFQEEGMFKTPTIAAPMMTKLTKLSILANNFAPTCDIPNAVTANLWGSVNWCNFFLEDRHVEKLKTSTKYIEQLNGNLRVFPSFYPAKLQNDDLQLKALFPMSEGLLIMRLDCALSPNDQQDFQGTCFFTAHDVFFYMNSTGFLSLVTVSISDLASFEIIDDKYISMYKTNELVMKIKVAPDVDLKAVVSKLNYLIENLALKDKPDDEKSLIQKFQKMDRDLDELQKKQTKMLVFYDEKLEYVKKASEVNPGNALQPLQKNQPTPAEKFGIVNLNIAEADITRRREKLAQSSLYVSKIDVPIEPQALMHLMFGENSVCFPKMFRFYNQNASDKSSTSVKPWVYYKENNAYMREFHLQLIYPNSFISKDQEEFSKNVIVQKINKIQSDYCEVETDHGDFYVPFVGTFRVKLFYVISRSPRVSGAAARSAEPSTIYRRSVLEAYMVIDNMDTGRGFWRDFLQNFMEKKVEEEIRVMKQIAHHYYTKMGNHGQLIKSVRIGGKISSRDTQREVAKWVDNRPMIQVHLWVLLRIISFRYLTSFVLFVIVGLKKVLTFFAALLTNITLLNKLVLTLLLLSGLGNLFLLGKSTISYWRVYKASQLANYHKNGGLVMERALYLNDLDLLTQEIVSPNKKDYVYEKFITTKEYETKYKDIRYEIAKRRNDLLVELKILSSMESEVMKGDFAQFVTNELQQCETVRLKYPDVWNDSNSTTLRKYCENCELKINKLEHNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGW9</accession>
    <name>A0A1E5RGW9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Target of rapamycin complex 1 subunit KOG1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1041</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat RAPTOR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGW9">
        <property type="interactions" value="860"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGW9"/>
    <dbReference type="InParanoid" id="A0A1E5RGW9"/>
    <dbReference type="OrthoDB" id="10262360at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030307">
        <property type="term" value="P:positive regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004083">
        <property type="entry name" value="Raptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029347">
        <property type="entry name" value="Raptor_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12848">
        <property type="entry name" value="REGULATORY-ASSOCIATED PROTEIN OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12848:SF16">
        <property type="entry name" value="REGULATORY-ASSOCIATED PROTEIN OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14538">
        <property type="entry name" value="Raptor_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01547">
        <property type="entry name" value="YEAST176DUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM01302">
        <property type="entry name" value="Raptor_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Raptor N-terminal CASPase-like" evidence="5">
        <location>
            <begin position="85"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="805"/>
            <end position="863"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ86150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1526" mass="172801" checksum="D00524A917E327B1" modified="2017-01-18" version="1">MSRPFTTTIPYGPHPLKPLNQETRHGFDDEYSSSAKLADLQNQFLFYFDDKRHETNGNPLPEQERAEDKDRFFQPIVRWKIGKERIKTVSAALILCLHLGVDPPDIVKTQPCARLEAWVDPMNFQDSKKAIEAVGKALQSKYELLSAKTKYKQSLDPCVEDLKRFCNNLRRASKQERILFHYNGHGVPKPTPSGEIWVFNRSYTQYIPVSLYDLQTWLGAPCVYVWDCNQAGSIIHNFQKFVAKRYKDDKDGKHDPAAPSSVEQYMDCYQLASCMHNELLPMNEELPADLFTACLTTPIEISVKVYLMQSPLKDTKYSVLFKNQDNIRESEKDTFTRLPDISIPGKSSDRRTPLGELNWIFTAITDTIAWSSLPRELFNKLFRHDLIVAALFRNFLLAKKLMVLYNCHPVSNPALPESISEHPLWKSWDLAIDQVLTKLIDSIKNPTAPNSQQMLLNPKQVNSDDSLTNSKSGLNSLSTMSLVNLQGGASQQHLLLQQNQAQQNGATLNGLQAHQQYSDFFVQNLTAFELWLKYGSNLRTAPEQLPIVLQVLLSQLHRSKALVLLSKFLDLGPWAVYLSLSIGIFPYVLKLLQGPSTEMKPILVFIWARIMSIDYKNTQTELIKEDGFMYFIRMISPEFSTSTRNLSAGLIMNVAANPSVRRFQHYHNHPSVAPSSPAVNATAPISDEQKAMAAFVLSAFVTDFELGQKYCFNLELIAQLCYLIEVSDTALLRQWCILLLGRLYERNPLYKYVILAHRDEPPYDIFAVLSRSLKDPVPEVRTSSLWSLTKFLAEIDETDISIKLLQDLQVQMNNIQQQLLQHQSIPPPSNSQSQHQQNSVSVHYQLQQQLQNIQSDIQTLQQTDVKEAKQQEVKLIVSALGVLNDGSPVVRKELVILLSKVVNTYMNFFIIIAFNELTEEIIALEKQNVQEKVAGNQIGQGSIFNTIWRSLLILSEDPHDEVKSLSSLVLDQILFSLNEHPDLGHIVLQMERYFVKRNEDKSVVNGGGKPTDLFKKNELSATSSASDLHKFQSSNAVNSKFGTEDHVSSNLTSRFLTAVKGWVAGADPSNMSDNDPATEKSMTKRLNKLSLLSTQYGMLPKARTARFCEKNLPAKIDKPLESNFFDYSKEYFQSPQMHKQEAEEPGSAEFQTRQWRIHRNDKIIRDTQEEKELSLYGDWSNLHCVIENKTPTKIFKFTQFDDYLVTGDDRDNIRCFDWNTKQTLCSFSNSNSYGTKITDLKFLNEDDNPLLLTCSSDGVMKIYKDFHDAALLTMISSWRGLTDMLLTPRSTGLLTEWQQSRGSFLVTGDVKVIRVWDALTESVETEIPAKTSSLVTSLTSDQLAGNIFVAGFSDGTLRCYDRRVDPRDSMIKLWKSGSSGAGSQGWINNVHMQRGGYRELVSGNNNGAIELWDIRHKDPVLGFSESNFKKNASMTTMQVHEHAPVIASGTRSVKLWTTSGDLLANFKNNSSPNSMVGTLTQVSRAPSYLSSMTFHPHRMMLAATNSHDTNICIYKCTDHKTSTNGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH05</accession>
    <name>A0A1E5RH05_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uridine permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1429</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH05">
        <property type="interactions" value="569"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RH05"/>
    <dbReference type="OrthoDB" id="2018619at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11482">
        <property type="entry name" value="SLC-NCS1sbd_NRT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4160.10:FF:000001">
        <property type="entry name" value="Uracil permease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012681">
        <property type="entry name" value="NCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045225">
        <property type="entry name" value="Uracil/uridine/allantoin_perm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00800">
        <property type="entry name" value="ncs1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618">
        <property type="entry name" value="NCS1 FAMILY PURINE/PYRIMIDINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618:SF5">
        <property type="entry name" value="URIDINE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="290"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="373"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="422"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="463"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="487"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="540"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="578"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="30"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="655"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="659"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="681" mass="77383" checksum="95836A3BA2C8C2DA" modified="2017-01-18" version="1">MINRVTSNASEVASAFDPFHSHFSNNNDNNDNFNNIDEIDYSNQNGNNKDKTTLDMINDDDGEDINKSXTPNTNLEEKENDLSSQSIWTKIIKFLEVKQNHVSGTSDSRSEDIMESFLFNDDLKPVEEARRVWDWKNYVFFWISGSFNVNTWQISATGLQLGLSWGLSWVTIWVGYISVAIFVSLGSRIGNFYHISFPISSRISYGVYFSLWIVLNRVFMAVIWFATQVYIMGQTVEVMLLAIFGTDLPERIKNTIGDTQPITTFQFMCFMIAWGVTLPFMWIQVHNLKYLFMVKSVIVPFAGFGFLIWTLVKFDGKVAVGSLSNFHPTGSLLGWNFIRSVMSAMDNFSTLILNAPDFSRFGKTPRSSVYSQLFILPVMYAIIAIIGIITTASAYTVYKVNYWSPLDILTRFLDHQTKGNKGGVFVIALAFCIAQLGTNIASNSISAGTDMTALLPKFINIRRGSYICAVISLAICPWNLMTSSSRFTTVLAAYATFLSSIAGVVSSDYFLVRKGYVRLLHCYTNKPGSLYMYGKYGTNWRAVVAYILALGLNFPGFIAEVSTAEVKVNENGRKIFYLNYYVGYLASFLFYFVLCYFWPIEGTRPNTKITDFKAFREVWVEVENFDEKRRIFLETGEEGEYDEYNDEYVFDNESRQENYGDSSSSSDKVQMQTEIVADKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH20</accession>
    <name>A0A1E5RH20_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CTD kinase subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1308</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH20">
        <property type="interactions" value="120"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH20"/>
    <dbReference type="InParanoid" id="A0A1E5RH20"/>
    <dbReference type="OrthoDB" id="4951845at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0195">Cyclin</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cyclin-like" evidence="2">
        <location>
            <begin position="40"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="330" mass="38824" checksum="10FFF49F436BA9EC" modified="2017-01-18" version="1">MSSKLILSRPFLTSSDIQNCQRNTISDRKVYNAKKVLIFRFLQEIVINFKFPRKTLEVTMYLYQRYYLFHSFEAGEIFDVATAXLYLGCKQVETMKKMNEIIPFCNGLKQDFLSSKINYDLEVYKKKLCKYEFELLNSLAFDSRIFDLNLSIDGLLLKFCKHSELSDRDSYMAWIVGLEVLKLEVLLSFPCHAVAMCCLQITKELVEQQQEDXTNSVLNIAKYNSLFQSLQLDKCNYSQCLSNIINFYLKMYDDCELSNIFSNVCTLDNFKTLQLKLCKEFPSRAEENSGANTEKPQITNDDELFETRYVVNRRYYDTEEHLILKKKQKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH78</accession>
    <name>A0A1E5RH78_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Polyamine oxidase FMS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1323</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH78"/>
    <dbReference type="InParanoid" id="A0A1E5RH78"/>
    <dbReference type="OrthoDB" id="5046242at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF410">
        <property type="entry name" value="LYSINE-SPECIFIC HISTONE DEMETHYLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="530" mass="59176" checksum="0F7EBD1E2C9DAF9C" modified="2017-01-18" version="1">MLDKSKKIVIVGAGIAGLKAASYLYAQGFTNLEILEARDRVGGRLYTVEGLNGKYDLGASWHHDLLINTLFKEEQQLGDKKGAPYVIEDDLVGLVDAEGRRLDCDPDLRLEPIREEFEDYVNEVYNPSLKKDTNLFQATINYLMDNKENLTHEQIKYMFGVARYYELWHGIDWKSISSFDAAGSHTGRNALCAGYIGIINRIGATIPLDQMLHLNTEVKEINVKYCPKTNKKYTEIEIQSGVNSSFKISKKRXIQADYVLITIPLSLLKLSLDEKGQKETGAIKFNPPFQPRLQRAIDNISYGSLGKIAFEFDKLCWSTEKSRMVYVGKNQDKLTSFIESNKKLTPELVAEMNTLTSSTAIPECESYPYFVVNLPKSLNGMPSLLFLCSEPVTVYLESLNGDVEKLFKFFEPALNSVLKSLRCKNKVVFHKNVSQNGKVGSTTPVLKNILCTSWTKDPYARGAYTAVKPGDDLEGFVEEIKKGHEDIVRFAGEACVVTGNGCTWGATTSAVRESDAIIADVAKTCRSAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH94</accession>
    <name>A0A1E5RH94_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein PLM2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1602</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RH94"/>
    <dbReference type="FunCoup" id="A0A1E5RH94">
        <property type="interactions" value="502"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH94"/>
    <dbReference type="InParanoid" id="A0A1E5RH94"/>
    <dbReference type="OrthoDB" id="5348546at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="203"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="571" mass="63980" checksum="23CB9454379FF127" modified="2017-01-18" version="1">MSGYGQFPPSSPAVNTIDYGTLSPNSSFHDGHDYDVTRKNELKTTKQQTGDDTESDDAMNANPAGDNSFILPTPHPSSALHNSSPIKKTRMDLPLGATGKIDDQKQPNVFLPAKTYLPALEKVDLTQVPVIAENEKSDVGENVQTKNCSTPVKSNSTFSVVSNVFLNSEQPQPKQLSYPLVHIPITYSLSAFKETGKMDPVEVRCGRKSAKNDISLPHDKWISREHLVISYIPQSVARAQSKNTVSHITKSTAKLPNVNIKCLGMNGCIVVYPKQMFEYELVQKGENVFELAHVSTITNGNGIATKQLIKKNDLTSFVIYQNEMVSMPFVKGMVLDFGKFEALIAPKNISKHQTQKKKQVLEIPRLASTPNDHINDPLSDITNTENNRTQISSIASDSIHKTENKAPFKANHSDSKMSATVKKKRKLPQDSPNREDNKESFHECKEIIEKSLENKEIDVPNMIRVLINHLAYASVQQVPLTSLFHVNTTISKLTKKELRYLLITKCECVGVIYRTGKDAAGKPLDEEYYYEFDKDTDQDRKAIVMSLKGGRANLRSCRKTHKQYFWKKPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHF5</accession>
    <name>A0A1E5RHF5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein YIF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1571</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Has a role in transport between endoplasmic reticulum and Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Golgi apparatus membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the YIF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHF5"/>
    <dbReference type="FunCoup" id="A0A1E5RHF5">
        <property type="interactions" value="512"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHF5"/>
    <dbReference type="InParanoid" id="A0A1E5RHF5"/>
    <dbReference type="OrthoDB" id="337750at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005578">
        <property type="entry name" value="Yif1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14083">
        <property type="entry name" value="YIP1 INTERACTING FACTOR HOMOLOG YIF1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14083:SF0">
        <property type="entry name" value="YIP1D-INTERACTING FACTOR 1, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03878">
        <property type="entry name" value="YIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="212"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="346"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ86013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="364" mass="41134" checksum="B91277F3C0027411" modified="2017-01-18" version="1">MSNPFSYSESSANNRSNRNSYMRAANAGNYGQVPVEELPPNGFSPQQPQQQAHQSHQYAAHNRNSSSEMPSFFQDPRAQVAYQFGQKAFSNFFQSDAQNPQEQQQPGGGLKSAANLVNNTSFFQLAYYFQVSNSYVFHKIKIIVLPFVNKTWYRVQDDTSNSDGSTGIVTFLPPRLDVNCPDMYIPVMGMITYILGCNLEQGFKGSYDPQNLYYKLSSTLAFVLLDLFILKLGLYLIIPNAIQNTPTGHRNGPGTTGVVITTPSSGSLLELLCFVGYKFVPLTAALFMRQSPVFSKLIFTAVRLYLFLGFGVFLLRSVKFNLLFPFVEHQTAVNFDRKSVIKKCNYFLFFYGFIWQVLLMWLMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHQ4</accession>
    <name>A0A1E5RHQ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Iron-sulfur clusters transporter ATM1, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Iron-sulfur clusters transporter atm1, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AWRI3579_g1020</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Performs an essential function in the generation of cytoplasmic iron-sulfur proteins by mediating the ATP-dependent export of Fe/S cluster precursors synthesized by NFS1 and other mitochondrial proteins. Hydrolyzes ATP. Binds glutathione and may function by transporting a glutathione-conjugated iron-sulfur compound.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the ABC transporter superfamily. ABCB family. Heavy Metal importer (TC 3.A.1.210) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHQ4"/>
    <dbReference type="FunCoup" id="A0A1E5RHQ4">
        <property type="interactions" value="684"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHQ4"/>
    <dbReference type="InParanoid" id="A0A1E5RHQ4"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140466">
        <property type="term" value="P:iron-sulfur cluster export from the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18582">
        <property type="entry name" value="ABC_6TM_ATM1_ABCB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000004">
        <property type="entry name" value="ATP-binding cassette sub-family B member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000287">
        <property type="entry name" value="Multidrug ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221:SF402">
        <property type="entry name" value="IRON-SULFUR CLUSTERS TRANSPORTER ABCB7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="249"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="19">
        <location>
            <begin position="133"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="18">
        <location>
            <begin position="474"/>
            <end position="712"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00045666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OEJ86431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="728" mass="82058" checksum="4F5C6CC13477D17B" modified="2017-01-18" version="1">MLLQLHPLFKPNMHAIKSIPKFNIASTCKTALFPKHRHYSKSNFHLGIYRKPASNLLSFNARSSVCPKSTLRLSIANSNNAFAVIKRNIHAGKPRMNAQKQSKAPSMSELKILKTLFKYIWPKNDNKVKFRVLIALSLLILAKVLNVEVPFFFKSIVDDMNIDWDAATKAENALPIAIVITILSYGAGRFGAVLFGELRNAVFSRVAQNAIRNVSLQTFKHLMKLDLNWHLSKQTGGLTRAMDRGTKGISYVLSSMVFHIIPITFEISVVCGILTYQFGSQFAAVTFATMLLYSFFTFRTTAWRTEFRKSANRADNKAATVAVDSLINFESVKYFNNEKYLADKYHKNLMNYRDSQIKVAQSLAFLNSGQNLIFTSALTLMMYMGCKGILLGDYSVGDLVLINQLVFQLSVPLNFLGSVYRELKQSLIDMESLFKIQTNEIKIQNCENPIKLLTPAERTNGSANGSDGIIPFEIRFENVTFGYNPERKILQNASFTIPAGLKTAIVGPSGSGKSTILKLAFRFYDPQEGKVFIDGKDVKTLDLDSLRRQIGVVPQDSPLFNDTIYENVKFGRIDASKEEILQAIKKAQMNQFIEKLPKGLETIVGERGLMISGGEKQRLAIARVLLKNTPIMFFDEATSALDTQTEQAILKTIKQNFKLEENRITSVYIAHRLRSIADADKIIVLENGKVKEQGSHQELLSRKESLYKDLWNVQENLDMLEEEIKADQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHT1</accession>
    <name>A0A1E5RHT1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">methionine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Methionyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g1038</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHT1"/>
    <dbReference type="FunCoup" id="A0A1E5RHT1">
        <property type="interactions" value="1070"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHT1"/>
    <dbReference type="InParanoid" id="A0A1E5RHT1"/>
    <dbReference type="OrthoDB" id="5844513at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017102">
        <property type="term" value="C:methionyl glutamyl tRNA synthetase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07957">
        <property type="entry name" value="Anticodon_Ia_Met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.20:FF:000001">
        <property type="entry name" value="Methionine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000037">
        <property type="entry name" value="Methionyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.20">
        <property type="entry name" value="Methionyl-tRNA synthetase, Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00098">
        <property type="entry name" value="Met_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041872">
        <property type="entry name" value="Anticodon_Met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018285">
        <property type="entry name" value="Met-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023458">
        <property type="entry name" value="Met-tRNA_ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029038">
        <property type="entry name" value="MetRS_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001100">
        <property type="entry name" value="PRK00133.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765">
        <property type="entry name" value="METHIONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765:SF1">
        <property type="entry name" value="METHIONINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19303">
        <property type="entry name" value="Anticodon_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09635">
        <property type="entry name" value="MetRS-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57770">
        <property type="entry name" value="Methionyl-tRNA synthetase (MetRS), Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methionyl-tRNA synthetase N-terminal heteromerisation" evidence="14">
        <location>
            <begin position="10"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Leucyl tRNA synthetase" evidence="13">
        <location>
            <begin position="192"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl-tRNA synthetase anticodon-binding" evidence="15">
        <location>
            <begin position="607"/>
            <end position="744"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF09334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF09635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF19303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ86479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="746" mass="84786" checksum="DA388AD95B0BC209" modified="2017-01-18" version="1">MKVTFDKNRNHSLALQKANNLKIALSLIKNPKLSVDEEAAKNTGSQLVDEKTGFALFNTNAILQYVFQDFSIKHPFAITNLEPILQTTKENNQHVEELVKKTIENYLSSDFDVNASNLIMFANAYALDFTNQFKNVQLPSVVQEALAILAKTMPRDSAQFKHTKASNIKQGHYVEIPKAGEKILPKENEKNILVTSALPYVNNVPHLGNIVGSVLSADIYARYCKSRNYNTLYICGTDEYGTATETKALEEKVSPKELCDKYHKIHAQVYDWFQIGFDHFGRTTTEQQTEIAQDILKKLEANGYLEEQSMKQLFCPVHKAFLADRYVEGTCPKCGYEDARGDQCDKCGGLLDPFELINPRCKLDNATPEPRVSDHIFLSLNKLENNIADWFEDSSTKGKWSKNAKTITKSWLKEGLNPRCITRDLVWGTPVPFEKFKNKVLYVWFDAPIGYVSITANYTEDWTKWWKNPENVKLYQFMGKDNVPFHSVIFPGSQLGTNENWTMLNHLNTTEYLQYEGGKFSKSRGVGVFGNNAQDIGVSPSVWRYYLASVRPETSDSQFSWSDFVARNNSELLANLGNFVNRLVKFVNAKYNGVVPKYDISHLPNFDALSTDINTILKQYIEVMELAQEKRGLELAMSLSARGNQFLQENKLDNSLFNDSPDKSDAVVGVGLNIINTLASVIYPFMPETSEKIYKMLNVPALKIDDTFHLTILGGHNINKAEYLFTRIDEKKIDEWRSKYSGQQVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHV2</accession>
    <name>A0A1E5RHV2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Serine/threonine-protein kinase svkA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1643</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHV2"/>
    <dbReference type="FunCoup" id="A0A1E5RHV2">
        <property type="interactions" value="756"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHV2"/>
    <dbReference type="InParanoid" id="A0A1E5RHV2"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF10">
        <property type="entry name" value="FI20177P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="22"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="477" mass="53876" checksum="FFB85062CBF84693" modified="2017-01-18" version="1">MDTDATESGFQPHKLSSNSKDYCLVSCIGRGSFGEVYKAIFKPTYVPSADGTISDDQFLAVKIIDMEESEDDIACIFTEVFFLANLKNNTNIVKYYKTFSIGSQLWIIMEYCGNGSCHGLMKKLPFDKFLNEKQISVIVKQILQGLQKLHYEQKVHRDIKLANVLISNSGAVKLTDFGVSAQIREKQQKLNTFVGSPNWMAPEVILNNFDKNYKKGYDEKIDIWSLGITVIELVEKRAPSPDKDVRKILQATIKRDPPTLNQCSRNISDSLKDFVNCCLQKDAEKRLSAEELLKHEFITKFAGCDEKGIVNDLLSSLLDHKGNNLEISANVFVDMSNNLTADSDELDKHNDTEFTFDSIDKSATGSFRQRANTFPNSAENHTPVSLQEEKPVEQSTIETSEQEKAAKLQSEPVGSYYYDKVVDVVFANVAKRCLNLDALNASQSLRALFRMTEKLQPGFSNVFVEELWLRIKSKQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHX6</accession>
    <name>A0A1E5RHX6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1282</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). The elongator complex catalyzes formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the ELP1/IKA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHX6">
        <property type="interactions" value="1114"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHX6"/>
    <dbReference type="InParanoid" id="A0A1E5RHX6"/>
    <dbReference type="OrthoDB" id="40048at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056167">
        <property type="entry name" value="A-sol_ELP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056164">
        <property type="entry name" value="Beta-prop_ELP1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056165">
        <property type="entry name" value="Beta-prop_ELP1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006849">
        <property type="entry name" value="Elp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056169">
        <property type="entry name" value="HB_ELP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056166">
        <property type="entry name" value="TPR_ELP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12747">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12747:SF0">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23925">
        <property type="entry name" value="A-sol_ELP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04762">
        <property type="entry name" value="Beta-prop_ELP1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23797">
        <property type="entry name" value="Beta-prop_ELP1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23936">
        <property type="entry name" value="HB_ELP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23878">
        <property type="entry name" value="TPR_ELP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017233">
        <property type="entry name" value="IKAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="ELP1 first N-terminal beta-propeller" evidence="7">
        <location>
            <begin position="1"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 N-terminal second beta-propeller" evidence="8">
        <location>
            <begin position="423"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 alpha-solenoid" evidence="10">
        <location>
            <begin position="736"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 alpha-solenoid" evidence="10">
        <location>
            <begin position="840"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 TPR" evidence="9">
        <location>
            <begin position="961"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 three-helical bundle" evidence="11">
        <location>
            <begin position="1135"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1210"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1225"/>
            <end position="1241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR017233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ86508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1345" mass="153238" checksum="0F8A753E250ABBDF" modified="2017-01-18" version="1">MRNLITFHRGNITPQSQTVENLQLLNSSFDTINDSITVVLGDLELGVIEVQQYSKDGTIAVLASFNLPVFHDKILSFVDFSDVNKLIFVMQQGDIICATYDPFSMDYTNTMVEIAGSIDNGLKAAKWSYDEETLALVSVDGKVILLSRDFDPINETELQLEDLKKSKHVNVGWGKKETQFRGKGVRAMEREALKSLQNSGLVGNQLRDPTMPYMVDEGKITSFDTKGVEISWRGDCEYFAVSSVETVEDADDSSKTLDRRVIRVYNREGQLDAASEPVDGMEHSLSWKPQGSLIASIQRKPFLSEEEEQSLDLIFFERNGLRHGEFDTRLPIDEPIKDLTWNXTSDILTIVLKDRIQLWTTKNYHWFLKQEIXSPGLVFCKWHPEKEFTLMFGDAXGINITQFAYKITNGPTVXGHDYGTTVVVDGQTVNITPLSIANVPPPIYYRDFDAPNAVLDVAVSYSNETYAAITNRELVIAKVDSVEQMRKGQHPSVRSTLDVSEFASNFDTLRQIAFIGENIVVILLDSESFSQLAMIDIQDPSQPTFLNIVDTYNKVVLLKSTFDYQNAVFETKIGEISQLTKEGQIIEITKLPQVVVDLRVSKIKTKAAETTSQILQNEYEDDANIWXSSAEEDSHYVAFGLTQNGKLFANDVQLTSAVTSLEISNNFLFFTTAQHNLQFVHLNSSSFKPLPIVDANVSDERVRSVERGSILVSVVPSKSMVVLQAARGNLESIHPRIMVLSEVRKHIKNKQFLDALLICRTHRISLDILHDYSPELFYDNLDYFINDIGRVDYLDLFISCLVEENVCETKYKETLNISSDEPFALLPAPLTDMQLYIKKKFFDAENSKANRICKAMLQTLLGKPEYVTKYLQSIITAYACQNPPNLKDALSLITTLQDEDRKESMVTYICFLQDVNVVYNVALSLYNIPLSLAIAQKSQMDPREYLPFLQELIDSEENQMKFLIDDHLGNYELAAYHLSNTELDQTEISENTLDYXEVHKLFKYALGLYKRDHKKQXQIYNIFAKELASTQEYSEAAIIYEMLGNYRTAMXNYVSCKKWNEALSLASQHFPDSVNEVAEELVTALNYEHKYVDSAQIELKYLKNVKEAIDLYCKAYYYHEAINICTFEPGSLGFIEEVVDSGLDEGFSVIAELLADCKGQVNSQVKRLRELREKKEQDPYAFYGEEIDQADDVSIAPSETSTRESFFTRYTGKTGGTAQTGTSRKTAKNKRRQERKKARGKKGTIYEEEYLVRSIGRLIDRLQTSQPDAIRLIEGLLRRHKMEQAYQIQKNYIEVLDLLKQNIVEIYTISEKDRERIDDNGEVYYIEEIPVPKMDEFPVNKVIDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHX7</accession>
    <name>A0A1E5RHX7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">pantothenate kinase</fullName>
            <ecNumber evidence="4">2.7.1.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g1647</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-pantothenate + ATP = (R)-4'-phosphopantothenate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16373"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29032"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 1/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the type II pantothenate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.33" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHX7"/>
    <dbReference type="FunCoup" id="A0A1E5RHX7">
        <property type="interactions" value="762"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHX7"/>
    <dbReference type="InParanoid" id="A0A1E5RHX7"/>
    <dbReference type="OrthoDB" id="498611at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004594">
        <property type="term" value="F:pantothenate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000025">
        <property type="entry name" value="pantothenate kinase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.10.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004567">
        <property type="entry name" value="Type_II_PanK"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00555">
        <property type="entry name" value="panK_eukar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280:SF20">
        <property type="entry name" value="4'-PHOSPHOPANTETHEINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280">
        <property type="entry name" value="PANTOTHENATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03630">
        <property type="entry name" value="Fumble"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PLAT" evidence="14">
        <location>
            <begin position="323"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ86484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="395" mass="44358" checksum="E00FE9572B461419" modified="2017-01-18" version="1">MSDNSSIVSNSRSKSSTSDSYNNISDLSIPLGSIPKKYLQMDMQRKIGIDIGGTLAKLVYFDDTRQCLDFKFIGTAEIEEFIDLIDQIIREKIEYRKQQEQQIQQETDKGIHQKDNFLDYSTSRIEIHATGGGAYKFNDSLVEKFGQDTIKRKDEMESAINGLTFLINYNDYLNEMTHIQEKEIFTYNNNDGKKYIEMEKVTRGSPNAKENTIMLVNIGSGVSMIKINRDTNEFERVGGSSLGGGTLWGLLSLMTGAETYDEMLELASQGDNSKVDMLVGDIYGSSYDNIGLKATHIASSMGKAFPKKNQMPDFNNSDICKSMLYAVSNNIGQISYLQSTIHHVDTIFFGGSYIRGHLMTMNTLNYAINFWSHGSSNAYFLEHEAYLGALGSIFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI12</accession>
    <name>A0A1E5RI12_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein L15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1488</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI12"/>
    <dbReference type="FunCoup" id="A0A1E5RI12">
        <property type="interactions" value="1351"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI12"/>
    <dbReference type="InParanoid" id="A0A1E5RI12"/>
    <dbReference type="OrthoDB" id="10255148at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1120.10:FF:000001">
        <property type="entry name" value="Ribosomal protein L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1120.10">
        <property type="entry name" value="Ribosomal protein l15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024794">
        <property type="entry name" value="Rbsml_eL15_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000439">
        <property type="entry name" value="Ribosomal_eL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020925">
        <property type="entry name" value="Ribosomal_eL15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003269">
        <property type="entry name" value="PRK04243.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847">
        <property type="entry name" value="RIBOSOMAL PROTEIN L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00827">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01384">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01194">
        <property type="entry name" value="RIBOSOMAL_L15E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ86559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="204" mass="24384" checksum="8CFF4A1A45898E4D" modified="2017-01-18" version="1">MGAYKYLEELQRKKQSDVLRFLQRVRVWEYRQKNVIHRASRPSRPEKARKLGYKAKQGFVVYRIRVRRGNRKRPAPKGATYGKPTNQGINELKYQRSLRATAEERVGRRCANLRVLNSYWVNQDSTYKYFEVILVDPEHKAIRRDARINWIVNPVHKHREARGLTATGKKSRGINKGHRFHNTSAGRRKTWKRQNTLSLWRYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI32</accession>
    <name>A0A1E5RI32_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">M-phase inducer phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1353</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI32"/>
    <dbReference type="InParanoid" id="A0A1E5RI32"/>
    <dbReference type="OrthoDB" id="26523at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010971">
        <property type="term" value="P:positive regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110032">
        <property type="term" value="P:positive regulation of G2/MI transition of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE DUAL SPECIFICITY PHOSPHATASE CDC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828:SF17">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="750"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="359"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="428"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="489"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="232"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="431"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="492"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="993" mass="111139" checksum="5C7C228B814919CA" modified="2017-01-18" version="1">MTSNSCIHKXEKVGKPTPFLFNPYISSSTCTSTSSSNSPSPKSIKFAKPPLPAYLGNSRSNSFNSGKFYRSKSISTQTTPYTDTVSWNLNMGHTRKNSGSTTAVLSGSPTIYENSRKHKSISSSVSSIFSYSSSSSSSLSKPHRHSHTPIHSHQTDLRKHHYQDQASXNRLGLSGHTQHSRNNSSQLNDSNTSLINCISDKIHNPKPTFFAPSPSYSSFSSSPSVALFNTQSLSHSRSSSRSTLNSQMKNKTNRNKNTYFIDTDEDENEYEDTYXDKEKQRMCSQQQAEEEDMNAFSNSYEETFDIDVGEYKFGGGNTNDDDSDDYIIQTPKNLNTRGVTAQGSTIKRVTSVISNISLPFTAGGSDDDDDDDDDDGLVATRSSDFNSASDCTNQTINDDDYTFMEDTISCANTPVLNDETGQVLLHPIESPFNSTSPRAVTQQEKQVLQSETSKDKKTPFYHLNHAHSLQKKKTYHYKNKLYRSSSLKKYNSHNAANSATNSTTTTNTMDVNNNKNNSTLSVFTDKNYKQNATFKHNDGLSNFRFEDPKSSSSLNLATTFGGNSALKNTKTAAASXNSSSSRVCELVRSNSQLRRKXQTPCVTPLTASFNNPNLNMSNLTTSSLVSTPASADRYTPVTPEKNNLLYKINTDTSFEISSTTSFLKSGCGAESSNETKITNNNERTNSTQFGDFPACPSSVSLAPSNANTSSSTENEAVSCAKFLDDTQREFCPNGIPYITCEEFEDVFKKKRENMVVVDCRFEEEYNAGHIINSTNIDDHINLKNFFFSNNAETASKTTSLNPFFQKSAHLEAQYEEPKLDNIMQKSNETLVVLYCEYTKYRSPTLAQFIRRFDRNLNLEYYPDLYYPNLYILKGGFYEFHHKFPQLCIGAYKPMLDSEKHEDKMDYFRSKSEFLLNSAQSSEIMLDMKLQCVKTLEQDESQEESDFYECSEALSSNMMLGQSSFSQGMKRNTSDFHSCLEIDTAHLDVQDTTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI46</accession>
    <name>A0A1E5RI46_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative nitroreductase HBN1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1664</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nitroreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI46"/>
    <dbReference type="FunCoup" id="A0A1E5RI46">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI46"/>
    <dbReference type="InParanoid" id="A0A1E5RI46"/>
    <dbReference type="OrthoDB" id="2138173at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.109.10:FF:000001">
        <property type="entry name" value="Nitroreductase family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="entry name" value="NADH Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033877">
        <property type="entry name" value="Frm2/Hbn1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43035">
        <property type="entry name" value="FATTY ACID REPRESSION MUTANT PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43035:SF1">
        <property type="entry name" value="FATTY ACID REPRESSION MUTANT PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="FMN-dependent nitroreductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="194" mass="20850" checksum="D3470D2265896A89" modified="2017-01-18" version="1">MSTQTILKAIASRRTIYALKPELPTGVALSDVQSIVQAIIKDTPTSFNSQVNRAIILTGDAHKKVWGQVVDGIEGDAGKKRPQSAKDEAYGSIIFFTDDATTEGLQAKFPAWSSAFPDFANHSTGAAQISTWTTLATINVFGHLQHFNGYIKAALGDAVPKTWTVHAQLVFGSKAGEPGEKTYIDNEVKVISSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI52</accession>
    <name>A0A1E5RI52_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">V-type proton ATPase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1192</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments. This subunit is a non-integral membrane component of the membrane pore domain and is required for proper assembly of the V0 sector. Might be involved in the regulated assembly of V1 subunits onto the membrane sector or alternatively may prevent the passage of protons through V0 pores.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the V-ATPase V0D/AC39 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI52"/>
    <dbReference type="FunCoup" id="A0A1E5RI52">
        <property type="interactions" value="846"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI52"/>
    <dbReference type="InParanoid" id="A0A1E5RI52"/>
    <dbReference type="OrthoDB" id="10250083at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.50">
        <property type="entry name" value="ATP synthase (C/AC39) subunit, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1690.10">
        <property type="entry name" value="V-type ATP synthase subunit C domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036079">
        <property type="entry name" value="ATPase_csu/dsu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002843">
        <property type="entry name" value="ATPase_V0-cplx_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016727">
        <property type="entry name" value="ATPase_V0-cplx_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035067">
        <property type="entry name" value="V-type_ATPase_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044911">
        <property type="entry name" value="V-type_ATPase_csu/dsu_dom_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11028">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT AC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01992">
        <property type="entry name" value="vATP-synt_AC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018497">
        <property type="entry name" value="V-ATP_synth_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103486">
        <property type="entry name" value="V-type ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR018497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ86567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="360" mass="41948" checksum="A118863DDD9D2669" modified="2017-01-18" version="1">MEGVFFNIDNGYLEGVVRGYRNGLINQSQYMNLCQCETLDDLKLQLSSTDYGSFLNTVPSDQLTTSVIQEYANNKLFNEFQYLMNNANEGTTKKFFDYLTYNYMIDNVILMINGTIHNRDKAELLSRCHPLGFFDTLPTLTVATDLETLYSMVLVDTPLSPYFKNCFGEDAANSQLDDLNIEIIKNKIYKEYLLDFYNWVSKSFPDYDPTKENMLAILKFEADRRTINILLNSLEHPDILDLDLKKQIVPELGRLYPIAYSQLLSSNDLDYENIKDIVESCVYEYKGIFDTNSNSDKNLEDYFYELEMEHCKDFFTQQFALSAVYAWLKSKEQEVRNITWIAECIAQNQKDKINNYIAVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI91</accession>
    <name>A0A1E5RI91_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">3-hydroxy-3-methylglutaryl coenzyme A reductase</fullName>
            <shortName evidence="9">HMG-CoA reductase</shortName>
            <ecNumber evidence="9">1.1.1.34</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1187</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(R)-mevalonate + 2 NADP(+) + CoA = (3S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15989"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.34"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Endoplasmic reticulum membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the HMG-CoA reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.34" evidence="9"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RI91">
        <property type="interactions" value="301"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI91"/>
    <dbReference type="InParanoid" id="A0A1E5RI91"/>
    <dbReference type="OrthoDB" id="310654at2759"/>
    <dbReference type="UniPathway" id="UPA00058">
        <property type="reaction ID" value="UER00103"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004420">
        <property type="term" value="F:hydroxymethylglutaryl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015936">
        <property type="term" value="P:coenzyme A metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00643">
        <property type="entry name" value="HMG-CoA_reductase_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3270.10:FF:000001">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.420:FF:000001">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.770.10:FF:000001">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.770.10">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl-coenzyme A Reductase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3270.10">
        <property type="entry name" value="HMGR, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.420">
        <property type="entry name" value="Hydroxymethylglutaryl-CoA reductase, class I/II, NAD/NADP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025583">
        <property type="entry name" value="HMG-CoA_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002202">
        <property type="entry name" value="HMG_CoA_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023074">
        <property type="entry name" value="HMG_CoA_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023076">
        <property type="entry name" value="HMG_CoA_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004554">
        <property type="entry name" value="HMG_CoA_Rdtase_eu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023282">
        <property type="entry name" value="HMG_CoA_Rdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009023">
        <property type="entry name" value="HMG_CoA_Rdtase_NAD(P)-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009029">
        <property type="entry name" value="HMG_CoA_Rdtase_sub-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053958">
        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00533">
        <property type="entry name" value="HMG_CoA_R_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572:SF24">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00368">
        <property type="entry name" value="HMG-CoA_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13323">
        <property type="entry name" value="HPIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12349">
        <property type="entry name" value="Sterol-sensing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00071">
        <property type="entry name" value="HMGCOARDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55035">
        <property type="entry name" value="NAD-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56542">
        <property type="entry name" value="Substrate-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00066">
        <property type="entry name" value="HMG_COA_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00318">
        <property type="entry name" value="HMG_COA_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01192">
        <property type="entry name" value="HMG_COA_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50065">
        <property type="entry name" value="HMG_COA_REDUCTASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0521">NADP</keyword>
    <keyword evidence="7 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="357"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="431"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="537"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="11">
        <location>
            <begin position="211"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="579"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1087"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="579"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="601"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1098"/>
            <end position="1111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ86607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1120" mass="123578" checksum="9D9DD407E34E24B5" modified="2017-01-18" version="1">MPLLSANTSKIAKPLAEFSRLAAKKPFQVILFTLILTSIAYLHVIEYYVNGWNIDNKPLFSSAQSNFNLNSHSVYDSKDFFYDSSLNTFQELXDISTIETEKNGHQQKNYFSTIPNYYLTELQFQLDSXIVGNTNTDKSALPHIDDVVYETQNSKFVLQSTFQLPDEVVSNDSSKKWKLRPQKKKLLQIKQWIVNLYVLFQEKLHQSDPVDLFVLGSAYIGMFYTFVKLFMDMRKTGSKLWMAVFSLVNSGCALFLAIYLSLHILEKPVTLLSLIEGIPFIVIIIGFKHKIKLAEFTLKQYSQSGFGNKRNVPSIMVNHAMQQEGGRLIQDYFICIFSFLACALFTQHLSILHNFSVLAAMILTLDLILTSTFFCSVLSLKLEINMIHRSTTIEQALEEDGILDQTAGIVAEQDLISENASFFTSNTIITFFKLLVTVAFVGLNFYNFAYRWTYESFTSIYKSDSNGSSMGMAGQSAKEYYQVLQILKSTVFDKDCIVSIVPTQYYEKTKLMYHVEDYIILIIHYISLAIRDKFISKFVFLCFCISVSINIYLLNTARIHTSITVKKYITKKNXKKLSSSSSANKATSSANKKPITNATMTTTTTTSSSSSSSSSSNKSVVSQAEEKPKLLKKSSSSSSLETFDPIYELEDVEDEIVRPLSDLEKLMKSGQLRSLNNQEVVSLVTNGKLPLYALEKQLADTTRAVVVRRKALSKLANAPVLETEVLPYKYYDYDRVFGACCENVIGFMPLPVGIIGPLVIDGVSYHIPMATTEGCLVASAMRGCKAINSGGGVVTVLTKDGMTRGPCVRFPSLQRSGACKIWLDSEEGQAKIKKAFNSTSRFARLQHIQTALAGDLLFVRFRTTTGDAMGMNMISKGVEYSLKMMIEELGWEDMEIVSVSGNYCMDKKPGAINWIEGRGKSVVAQAIIPKLTVEKVLKSDVKALVELNISKNLIGSAMAGSVGGFNAHAANLVTAVYLALGQDPAQNVESSNCITLMKETGNGDLQISVSMPSIEVGTIGGGTILGPQGSMLDLLGIRGPHPTSPGSNARQLAKIVACAVLAGELSLCSALAAGHLVQSHMIHNRAKAPPSTSVESGKSAEKTPQELETLKKGSVTCIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIM8</accession>
    <name>A0A1E5RIM8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">serine C-palmitoyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.50</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1028</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.50" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RIM8"/>
    <dbReference type="FunCoup" id="A0A1E5RIM8">
        <property type="interactions" value="907"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RIM8"/>
    <dbReference type="InParanoid" id="A0A1E5RIM8"/>
    <dbReference type="OrthoDB" id="3168162at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004758">
        <property type="term" value="F:serine C-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF2">
        <property type="entry name" value="SERINE PALMITOYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="11">
        <location>
            <begin position="213"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ86433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="611" mass="68488" checksum="8B99A42B05115203" modified="2017-01-18" version="1">MMEDTVVTKTITSVSTSMVSHLCNRVLNITIKNNHVSTNTGYDSTGLNTRLTIKDILIKMAQFPVLRYIINYIKKSHQNDPYRTLVEILLIAVGVLYINMKKNTMSVANTKNSNGGGRNNDGQLQLSAKEQEQLLKEWQPDALVDELDDEYSKWKLNGQVTLLTRKNKAKTNEADTSKMNTSTNTTSLTQRDVEYQGQVVQDVYNAANLNFLDLQNSPKLVSKIKTTLKNYGVGACGPAGFYGNVDVHYNLEYDLAHFFQTENAVLYGQDFCVAASVLSTFNKRGDIIVADNGCCVSIQNALQLSRASIYHYQHNDMQSLEKILAKIDKDEKFEPEIHRKFIVTEGIFQNNGDLCPLPELINLKHKYNFRLFIDETLSLGSLGSHGRGIVEHYGFPNRSNEIVDITVGSMATAFGSSGGIVLGDNVMSFFQRIGSNAYCFSASLPPYCCVAVSESMRILENDPLCVTKLHENTKFLYICLQKKLTQSNCKFVLTSSPESPLLIIKMNQTLRSKLFNSSMQSIYASLNNQKNVLHVTNTQIKEFNNEEMYLQKIVETLLLQYKVLIALTPHSLQHETLPLLPSLTVTTTAAFTAKEIEYIAESIGEVLVKFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RJ19</accession>
    <name>A0A1E5RJ19_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription activator GCR1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1468</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RJ19">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RJ19"/>
    <dbReference type="InParanoid" id="A0A1E5RJ19"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060963">
        <property type="term" value="P:positive regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052146">
        <property type="entry name" value="HOT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022210">
        <property type="entry name" value="TF_GCR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37784">
        <property type="entry name" value="PROTEIN MSN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37784:SF4">
        <property type="entry name" value="TRANSCRIPTION FACTOR-LIKE PROTEIN EUC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12550">
        <property type="entry name" value="GCR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription activator GCR1-like" evidence="2">
        <location>
            <begin position="252"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="442"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="443"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="461" mass="51880" checksum="C0B07AB745CC9A5D" modified="2017-01-18" version="1">MNGQSNGDREGARSTGSNNSLFSAQQEQNDRDRTIHTGTQPITTNSRNTFAARLSNTNGPLNNTTASPHSSSINASEEIFCLSSFLVDQVEKIQENVHQISKEQDLFHQSTNAKIAXMETKFDTLTNEMKSLLGFFHEVVGVMNGARAWTSSHPTTNTAISHETSGPIVGNQNKTFAQATNTVEKQPHDLTANVRNAFNSTVTESLDRDEHIVSQSRLKREPLTDKDLLRRIGDGHIYVREKEKKDKPINVYRLNRKLESVADVAQEYFHGLQHQPSVQSLDARYGTSWRKTDRSFYSKRMTIINKIKHVKENPGSYDLPADMTLSMATRVIENIRLGNNTLSKDNPDGCIKLSLTGLYTYFQKKQDIVSDYSLILREEGKTRQEIALRKRYGQIGHNTYAPGSDTSDSRQRISSAAQMNVXTGHVTLPYVRRSRPYHPMLNQEASNENASDFDSSGDYSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNB5</accession>
    <name>A0A1E5RNB5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcription factor tau subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g706</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNB5"/>
    <dbReference type="FunCoup" id="A0A1E5RNB5">
        <property type="interactions" value="128"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNB5"/>
    <dbReference type="InParanoid" id="A0A1E5RNB5"/>
    <dbReference type="OrthoDB" id="414418at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000034">
        <property type="entry name" value="Transcription factor TFIIIC subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051710">
        <property type="entry name" value="Phosphatase_SH3-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014623">
        <property type="entry name" value="Tfc7/tau55"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019481">
        <property type="entry name" value="TFIIIC_triple_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16469:SF51">
        <property type="entry name" value="TRANSCRIPTION FACTOR TAU 55 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16469">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED AND SH3 DOMAIN-CONTAINING BA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10419">
        <property type="entry name" value="TFIIIC_sub6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036802">
        <property type="entry name" value="Tau55_TFC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor TFIIIC triple barrel" evidence="2">
        <location>
            <begin position="311"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="268"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="413"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="447" mass="50161" checksum="89D890AC76757EA1" modified="2017-01-18" version="1">MTVKTIYIARHGYRSNWLPNGPYPPPPTGVDSDVPLAAHGVQQAQELAHYIMSIPDADKPELLFSSPFYRCVQTIYPTSELLDVDITIERGLGEWFKPDRDVVPVPTSVQEMQKLFVNKKTKLEPEMWSTVIPNTNGETEELIRVRCHEFWPLFIQKIEKEYPDCEKIMLCTHAATKIALGMNLLGFSDLKESIDDKGSTIRSGACSIDKYELLFDGDASDNFYEKNWVLTMNGNTQFLSAGEEMHWDFRNGFEAGSDADIAYRRKLQESKEGKQDPNNTKMDVLGDKNGDTANATKADKKDEGDDSDEYETEEVYVSVNLPQNEFRTYNKIDTKNTTFQSSGLETKEPLMKIGDKVYKGKWNRLVGTEVVMDSGSSSVKKELDGKTPGASDEASPALDGNTEHKSDKKDATANGTEKSTPQSNSEEEGTVVKVVEHELQLEEVHLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RND3</accession>
    <name>A0A1E5RND3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">U3 small nucleolar RNA-associated protein 25</fullName>
            <shortName evidence="9">U3 snoRNA-associated protein 25</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g847</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">DEAD-box RNA helicase-like protein required for pre-18S rRNA processing, specifically at sites A0, A1, and A2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Nucleus</location>
            <location evidence="2 9">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the UTP25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RND3"/>
    <dbReference type="FunCoup" id="A0A1E5RND3">
        <property type="interactions" value="1276"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RND3"/>
    <dbReference type="InParanoid" id="A0A1E5RND3"/>
    <dbReference type="OrthoDB" id="10264378at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010678">
        <property type="entry name" value="UTP25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053939">
        <property type="entry name" value="UTP25_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053940">
        <property type="entry name" value="UTP25_NTPase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12933">
        <property type="entry name" value="ORF PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12933:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 25 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06862">
        <property type="entry name" value="Utp25_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22916">
        <property type="entry name" value="UTP25_NTPase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="6 9" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="UTP25 NTP hydrolase-like" evidence="12">
        <location>
            <begin position="271"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="UTP25 NTP hydrolase-like" evidence="12">
        <location>
            <begin position="466"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="UTP25 C-terminal" evidence="11">
        <location>
            <begin position="576"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="129"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="33"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="56"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ88399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="770" mass="90410" checksum="C7C6E173B6BB93EC" modified="2017-01-18" version="1">MNNYKKHGRKELRTIRRNTQRGNEDVDSTVSLNDKKDFDYDKSERDHSGDMSQSDGELEPETAVEEDESERKTKVYDALLTLLGPSEEEKAEKNHDYTVALNSELGENNKRKKINIMTEEELLDDAMEDGYAQEEEEEEEEEESKELETNEDIDSDEEHDEYNVHFNDYSEDYIRNLNDGFKNKTVKQKSTKIPIYSNPEDEEDFENLIYSKPTLIKDLDEKMEPVKLAHSTKPYSIKQKLKYANEDLVHGSGNAALTDVQKTILDPIFQYKDLLYEYDSHDKESEYRDVYALHVLNHIYKTRDKILKNNAKLQENSDLELLDQGFTRPKALIVAPTRNCAYDIINTIIKRSGLDQIDKRGKFHDQFFTEDAPFNENKPKSFQHVFKGNSNDFFIIGLKFTRKAIRLYSNFYQSDLIVCSPLGLNLLLQTYSYESKDKENEDEDRKKVDAMNKHHKSKKEKFFKKKKGGKKNSDDFLSSIEILVVDQLHSLELQNFQHLYNLMTKHINNIPKEQHDNMDFSRIKMWYIDDLAKLFRQTLIFTRYLTPWSNLLINNSLNYAGKMKNLKNINSKSSAIDSIGFRVKQIFQRFDFSSNSIQEEPDYRFKYFTSVIIPQLIVKSTGYDEGILLYIPDYSDYMRLRNYFKKHIPILFEEINEYSSQSQLSSARSSFANGRRGKILLYTERLHHYRRYEIRGCKSVFFYKPPSNPEFYREIVNFIGKSVFRGECDINISNVRIVYSKFDGLALSRIVGSKRAAILTHGSNEVYEFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNH6</accession>
    <name>A0A1E5RNH6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g922</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNH6"/>
    <dbReference type="FunCoup" id="A0A1E5RNH6">
        <property type="interactions" value="161"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNH6"/>
    <dbReference type="InParanoid" id="A0A1E5RNH6"/>
    <dbReference type="OrthoDB" id="3972213at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056790">
        <property type="entry name" value="Ribophorin_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008814">
        <property type="entry name" value="Swp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12640:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12640">
        <property type="entry name" value="RIBOPHORIN II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25147">
        <property type="entry name" value="Ribophorin_II_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044292341" evidence="8">
        <location>
            <begin position="23"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Ribophorin II C-terminal" evidence="9">
        <location>
            <begin position="193"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ88436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="297" mass="33653" checksum="19EB450B1E89C25F" modified="2017-01-18" version="1">MQLLSWIYTVFVAVSMLNSANAITNPQLLAGTSKQFLDLSQGPQKITLDYNTNDLLKFQFEYIPSPAPKTNDEQEDAPIVANTKPQHASLVLSVDNKIEQPYMIKSSKDNVYSWLLQSSQLPNPLVYYSTKWGKPISGKLYFKSAEEAIVNELFFEIEFDKTTISDQTLSSLIVEAPEQEKKNSVKPEIIHQFHAPPKQANKFIVSIFIVAVLLGALFTLVSWFAAIDFSKNHITLRKQCGTLVYLAGFLVGILGLEFSFFQYYLGNNDIFRTLYNVFVLFTLPTMYAGAKLLKRLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNI2</accession>
    <name>A0A1E5RNI2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ATPase GET3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g940</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1 and GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-GET2 receptor, and returning it to the cytosol to initiate a new round of targeting. Cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer. Component of the Golgi to ER traffic (GET) complex, which is composed of GET1, GET2 and GET3. Within the complex, GET1 and GET2 form a heterotetramer which is stabilized by phosphatidylinositol binding and which binds to the GET3 homodimer. Interacts with the chloride channel protein GEF1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Golgi apparatus</location>
        </subcellularLocation>
        <text evidence="11">GET1 and GET2 are required for targeting GET3 to the endoplasmic reticulum.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the arsA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNI2"/>
    <dbReference type="FunCoup" id="A0A1E5RNI2">
        <property type="interactions" value="983"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNI2"/>
    <dbReference type="InParanoid" id="A0A1E5RNI2"/>
    <dbReference type="OrthoDB" id="1770at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043529">
        <property type="term" value="C:GET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071816">
        <property type="term" value="P:tail-anchored membrane protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02035">
        <property type="entry name" value="ArsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001359">
        <property type="entry name" value="ATPase GET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03112">
        <property type="entry name" value="Asna1_Get3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025723">
        <property type="entry name" value="ArsA/GET3_ATPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016300">
        <property type="entry name" value="ATPase_ArsA/GET3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027542">
        <property type="entry name" value="ATPase_ArsA/GET3_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00345">
        <property type="entry name" value="GET3_arsA_TRC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803">
        <property type="entry name" value="ARSENICAL PUMP-DRIVING ATPASE ARSENITE-TRANSLOCATING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803:SF3">
        <property type="entry name" value="ATPASE GET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02374">
        <property type="entry name" value="ArsA_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0813">Transport</keyword>
    <keyword evidence="8 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ArsA/GET3 Anion-transporting ATPase-like" evidence="12">
        <location>
            <begin position="18"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="26"/>
            <end position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ88441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="351" mass="39069" checksum="5C8F5D912882C1E4" modified="2017-01-18" version="1">MDFTVDPNLSDLINSTTHKWIFVGGKGGVGKTTSSCSIAIQMATKQPEKQFLLISTDPAHNLSDAFGEKFGKDARKVTGMENLSCMEIDPSAALKDMNDMAVAQNNSNGSNGGLADMLGGGGALADLTGSIPGIDEALSFMEVMKHIKKQEEGEMSYDCVIFDTAPTGHTLRFLQLPTTLTKLLDKFGEMTSKLGPMLNSLTGGGNMDLTGQMNELKVNVEKIREQFTDPDLTTFVCVCISEFLSLYETERLIQELNSYDMEVSSIIVNQLLFAEYDEEHNCKRCQSRWKMQKKYLSQIDDLYEDFHVVKMPLCAGEIRGLNNLKKFSQFLYKEYDPVADGKVIYELEDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNJ0</accession>
    <name>A0A1E5RNJ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g822</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNJ0"/>
    <dbReference type="FunCoup" id="A0A1E5RNJ0">
        <property type="interactions" value="104"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNJ0"/>
    <dbReference type="InParanoid" id="A0A1E5RNJ0"/>
    <dbReference type="OrthoDB" id="426001at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF519">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE MPK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="175"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="256"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="11"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ88451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="412" mass="45587" checksum="DD4EA48CC80CEA33" modified="2017-01-18" version="1">MNEDLEKNKPSLLSNSSRNDSSVHSSKQPVSATRVTLHKTISAANIYSRSNSYTNIAGTNVYNKNALNASKTRAKTLVGTANSFTQAGTRPRSSTFLPTLTIPTGNIKADSSPTLKSALSTQHIRNRSQTLPVHSNLLEEKSALTGNNTYNPILNSEKEHNPLAFNNEQTAYPNGPIKVIEPNIYLCSQASEPIAVNFDLVINVAEELPELNLGQNQTATQHKSNQPDFLHKYWNHDTEIIQDLPTLTSLMHSYAESYESNTRQQTNLSKPTSKKEKKVLVYCQCGVSRSASLIVAYYMRYYKKSFQEAYKHIQSIATSISPNMSLICQLSQWDTVLKSTNYNPISPHKYGIDVFEAYDIATPLEEADDGNVSSATLFTNSSKSSPMSWDTPATPVNCVLSSNPFLKNHPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNU0</accession>
    <name>A0A1E5RNU0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Calcium-binding mitochondrial carrier SAL1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g777</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNU0"/>
    <dbReference type="FunCoup" id="A0A1E5RNU0">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNU0"/>
    <dbReference type="InParanoid" id="A0A1E5RNU0"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000016">
        <property type="entry name" value="Solute carrier family 25 member 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="391"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="471"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="521"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="57"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="117"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="296"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="388"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="518"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="17"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ88538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="614" mass="69818" checksum="D73F67D754B8C604" modified="2017-01-18" version="1">MGDGDKQDDLITTNGNTDKKSSMENNAEHSKNNTKLQNIEIKNKYPQNNESRSEIQEQIEEINQLFNILDYQNKGEISFKDFKHAIRNSDLIEKNYHPRGDVNKHVTANLHNLLSNYSNKTIYELFQKIDIDQDEFIDKQDFIQYYEKTEAQIKLGFQKIDLDKDGLIKSNDILKYLDGLMNSVNTKETNTTNKSDGDINNSLNETNYARIRKEFVKWAFLGNTRFNDPNFNTKDVSNANTNTQGITYDDFKLFLLFIPRNDGSRLKTAFNHYYYEYFLNKSDLDLSSDSDIIYINNSLKFFIAGGTSGVISRSCTAPLDRIKVFLIANEKNLHVWEVVKQIYRKGGGWKSFYVGNGLNSLKVFPESAIKFGTFELVKKLCFGKQSDDNPIVPYICGGLAGVMAQLSVYPIDTIKYRMQCENIKMSPSHPINNLLHPQASTASSLSSTKKMNNINIMKIIKDVYKINGIRSFYRGLNAGLMGMFPYAAIDLGTFNLMKNFFINKQCAEQGVRREDVVLPNILLLPMGAFSGSIGASIVYPINLLRTRLQAQGTFAHPHTYLGFFDCAKKTVMREGYRGLFKGLAPNLAKVCPAVGISYFCYENLKKALNIDSTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNU5</accession>
    <name>A0A1E5RNU5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DNA mismatch repair protein PMS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g779</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNU5"/>
    <dbReference type="FunCoup" id="A0A1E5RNU5">
        <property type="interactions" value="758"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNU5"/>
    <dbReference type="InParanoid" id="A0A1E5RNU5"/>
    <dbReference type="OrthoDB" id="10263226at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032389">
        <property type="term" value="C:MutLalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061982">
        <property type="term" value="P:meiosis I cell cycle process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16926">
        <property type="entry name" value="HATPase_MutL-MLH-PMS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03484">
        <property type="entry name" value="MutL_Trans_hPMS_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.100:FF:000001">
        <property type="entry name" value="Mismatch repair endonuclease pms1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000017">
        <property type="entry name" value="PMS1 homolog 1, mismatch repair system component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.20">
        <property type="entry name" value="MutL, C-terminal domain, dimerisation subdomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014762">
        <property type="entry name" value="DNA_mismatch_repair_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013507">
        <property type="entry name" value="DNA_mismatch_S5_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002099">
        <property type="entry name" value="MutL/Mlh/PMS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014790">
        <property type="entry name" value="MutL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042120">
        <property type="entry name" value="MutL_C_dimsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037198">
        <property type="entry name" value="MutL_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00585">
        <property type="entry name" value="mutl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH, PMS, MUTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073:SF52">
        <property type="entry name" value="MISMATCH REPAIR ENDONUCLEASE PMS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01119">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08676">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01340">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00853">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118116">
        <property type="entry name" value="DNA mismatch repair protein MutL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00058">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair protein S5" evidence="5">
        <location>
            <begin position="219"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="MutL C-terminal dimerisation" evidence="4">
        <location>
            <begin position="740"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="371"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="601"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="389"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="405"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="613"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="662"/>
            <end position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ88547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="948" mass="107583" checksum="B157A1C5994F2174" modified="2017-01-18" version="1">MSIKAITSNDIHAITSGQVITDLQSCIKELLENSIDAHAKQIDITVRNYGCDSIEIQDDGDGIDRKDFESLCLKNYTSKISAFEDIASEQFSTLGFRGEALSSICCISKLRIVSATADTTPIAYKLEYDASGKLLTNKKTSRNRGTTTTVEDFFYNLPVRRKNLLKNNKRSFQKCVQMIQDYCIINSHINIKCWHITASNRKNLVLANSSRPTTLMKNLVVLFGAQSLKGVEDINCTILVDAKKSFIEEYNFDGGEVLLSGCISKHTFGCGRTSKDKQFIFINKRPVDYPKIVNVVNEAYKTFNNVQFPFFVLNFEINANLLDLNVTPDKKTVLIHNEPSFLSNLRDCLIEYYNKQEMTFFPNSQVMKQSQVHDSAGATQIERKRKLEFDDEKEDVEDEADPEESSQQTPLSTPSVSSTNVSQYQIEVSNPTTVPAPALNFSEEISDSTLKNVKAMQYDNNLSFQESQKKSLSNEPHHQTSDLEKDLEAIQKDDIREEGYYRKTITSTNSRDFRKGFQTRIDAFAHRVEPAHSHEHCHHGGLFASDDEEELANEQEEEIVIDVAGTQTVEKIKSPYASPFSKSRSKSVDTNVLKREAGTKIGLTLGKEQEAEAKEEEEGEEDYDNDDEVKEENDDEAFSEDSEEALPQVLNERVPQPLGGLSTFSRPSSSQQEESNLHSYEAKIGANLQSISNSCKNSATFWQQKHMSLFNKNENIANKQQGEEYLTLTVSKSDFTQMKIVGQFNLGFIICTRQKTKDGKYDLFIIDQHASDEKFNFENLQKTTKFKSQALIQPKIMELSVIDELIVLDNLKTFELNGFRLKTQDANDDVFVGDTAENKGKIAIVSLPFSKKTQFSFEDFYELVHLVKNSSNHSNETIRCSKIRAMFAMRSCRMSIMVGKPLTMKTMEKVVNNLSLLDKPWNCPHGRPTMRHLMELKDWDSFNADYEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNU8</accession>
    <name>A0A1E5RNU8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">J domain-containing protein APJ1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g785</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNU8"/>
    <dbReference type="STRING" id="56408.A0A1E5RNU8"/>
    <dbReference type="InParanoid" id="A0A1E5RNU8"/>
    <dbReference type="OrthoDB" id="550424at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044713">
        <property type="entry name" value="DNJA1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43888">
        <property type="entry name" value="DNAJ-LIKE-2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="11"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="8">
        <location>
            <begin position="257"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="5">
        <location>
            <begin position="257"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="181"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="506"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="181"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="506"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="528"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="540"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="560"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ88550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="600" mass="67464" checksum="661D84B98DCE945C" modified="2017-01-18" version="1">MSSDDPVSQVNPYEILGVARDADFETIRKAYKGMALKHHPDKNNHSETSNRKFQEICTSFNILKDQTKRYYFDKYGTIDEHRISEMQQKEQLQRQRNKRQKRTNATGGYANTGMSAAATFSEFFNFANSFGHFSSSPFGSSTTNSSVKFPSAFGSSTTGFPSADSFFNSSFFDTFNMGTSTNRNRSSTNTSANSNVNASNNNNNNNNNNNNNNDNYNAKNQRTTRKPAFGTSENQAYKRGPNISHNLKISLVDLYEGKTLKLKMSRSKICETCCGTEATGYMTFCNKCEGTGTITKMDFDSAWQMLQQAYCTKCDGEGKYFHTVSECKTCFGQGYVNERKIFNVEVTKGMKYFEQIVLQSQADEVIALGNGDYQGCIPGNVVITLVPSDESVVLSKNKNIITKEENKSTKCFVISGGKDLLIDNLSASLVDILCGGVITIPSNIHPKRHGILKLQLKPNSLPNPYGIVVFKNLGMPLDPTGDLYGDLYVRFKILFPQNLNMQQQAQLKSVLTSNSNETTDKKKKKNNNDNNNNIYNNDDNDGKDKTQMEHGTDQHHHQEEEEEEEDDDDDDDDVIEEYELYGMSNVINLDEFIEMIKVTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVH7</accession>
    <name>A0A1E5RVH7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Transcriptional factor SWI5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g326</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVH7"/>
    <dbReference type="InParanoid" id="A0A1E5RVH7"/>
    <dbReference type="OrthoDB" id="3437960at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001752">
        <property type="entry name" value="Transcriptional factor SWI5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051061">
        <property type="entry name" value="Zinc_finger_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179:SF13">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="835"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="865"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="436"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="659"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="930"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="223"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="440"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="457"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="671"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="744"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="761"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="773"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="967"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1051"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1075"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="13">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ90778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1119" mass="124505" checksum="5739F0C7E7169E2C" modified="2017-01-18" version="1">MDYNYHNWQNQQTGFANSMNPQGHVEAPFNTTSKFMVGKNVRIPSSVFLTDANNIDSSKQNNAFGYANDAANPQVILAKHNSTISTGGNLKFDPSGVPDISHSQLPLPDDDYNDFFDLNLEDFGMNDDDILANIDNLEIPSIPQESELQQAIQKQTKNIANKLDLKNDDFETVQNFKSTSKRKVSGTAIFGFHNHDKTLLPENNSISVQANGGLSSQYGKDDVPSAALELENQQKKLKEALEQQKILNLQLSEQLKMNQLQQEKLQSELSKQKQIMSSPNRELTTDGKMYVTSNSPQGKYAFPTPYGAYGFPQRRDTYFSNSSSPIRPPREQQHAQDMLDLDSVSLQIDTLNGSPVRKNGLMTQQQQQSSMNGSFVNGGNGSALFGHPRLMQQQNGYSYGAQEYNNNHYFEGPPVQSNTEFDAQNSVLVVKKQPRAMSKTKFENSENEQAEMRKKSTSSNSTSHSTIPQEFTPVMSYPHGNKSNENNRNFVNNHVNRTPAPSGSIGLGLMRMAANPSSELQNKEVVHNFEHPRDLNFGGGRRESTTSGCSREDLLNSIVLKPPSFHDISCDTLKNNSTKTSCVEEEEPESKFVQAQTPSPILKSQDKFEGSSSVNFDVPKKTVIXHAQKGSNGLEYQKHAFVNKRNDLYITPKLQSTPIFATGHGPESHNSRNGGTNQYGGLSKNSSPLGFENAPLPSPTMKYVSIPSPVSSSGAGKHNHNHNRSPKLGCSPNVPKLTAGTFDDNGHKHXHEYDHEXDHGYDDDDDDDNDDDHEGHGHGHGRDGDVEMDIHNNEFAGDGTTIGIINMSPKRIRKSSTLPPGTIDQYVVELPDKTFVCHYNQCWKKFHRRYNIRAHIQTHLQDKPHKCDYPGCTKSFVRNHDLKRHKKSHLEKTHKCECGKKFNREDALEVHRERKKCLLKGSNKKIVKKTEEQLDLSSDGEEDKDENAKVDGPPINDNYIHHDNNINHDNNINHDNNINHDNNINHDNNQEIKISCTPSVSKGAGVNKSPRKKQRLNAKPQLPKSTGSNHMIMGMHKSETLSCDGTVRRPSSSSSSNSSSSSSGGGSSSSNKSNDGTSAANNPSPVKENIRKTILKNGEQDIALKVESLLMSKQQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVH9</accession>
    <name>A0A1E5RVH9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Pore membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g493</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PER33/POM33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVH9"/>
    <dbReference type="FunCoup" id="A0A1E5RVH9">
        <property type="interactions" value="166"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVH9"/>
    <dbReference type="InParanoid" id="A0A1E5RVH9"/>
    <dbReference type="OrthoDB" id="3972494at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071786">
        <property type="term" value="P:endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051645">
        <property type="entry name" value="PER33/POM33_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005344">
        <property type="entry name" value="TMEM33/Pom33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12703">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12703:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03661">
        <property type="entry name" value="TMEM33_Pom33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="5"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ90947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="274" mass="31786" checksum="20E30F74D907E1AC" modified="2017-01-18" version="1">MNLQLIWFIGHCFTLLGTIFFILSGFSSYSSYTFLYTGVILSFGIQLYQQYFKQLIKSTPSSQDKTSTTTSRFARGRIVIRDVNFPYFTMALIWLITPITGLGVFPYAIFSVFHALTYIKNVLLPQRFPHLNKYAPYINAFLQRYQDLSMTLSANFEVYCLLLLIVKNLMFWNWSVVKTVQFVGYVLFIKLRLESSKVLVSAFAHLGFQFETFLARTQQQNPTNNFIHHIKNYYITLKMRCKNFNAFNLTNYLAGPPPQASSSSSTPSNERKQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVM0</accession>
    <name>A0A1E5RVM0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pre-mRNA-splicing factor CLF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g345</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the crooked-neck family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVM0">
        <property type="interactions" value="1099"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVM0"/>
    <dbReference type="InParanoid" id="A0A1E5RVM0"/>
    <dbReference type="OrthoDB" id="541719at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059164">
        <property type="entry name" value="HAT_PRP39_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045075">
        <property type="entry name" value="Syf1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246:SF3">
        <property type="entry name" value="CROOKED NECK-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23241">
        <property type="entry name" value="HAT_PRP39_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23240">
        <property type="entry name" value="HAT_PRP39_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ90987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="678" mass="81896" checksum="FEFA9605D04ED56D" modified="2017-01-18" version="1">MEVLRNQGAADFSPDELLKQAYQKVNVPKKSLAELEIEDLEELKYFQRLKRSEYEDYLKRNRLDLKQWIRYGDYEVMQCDMRRARSVFERALRVDNTDIPLWIHYVDAELKNCSINHARNLLERAIKLQPNVAKFRFKYLYVEENLQNFNLVREIFEKWLHWDPHNEMCFDSYIAFEMRYQEHGKARALFARYAESIPKSYTWLKWINFEKQLCEAGHIRDIFDNAIDFFDFNKRYDELEIILQHYADWEAQENEIERSTAIINLCQSKCNLSPEAYQRYAKLYLPYTDILEMKLLEYQHFVEXYPKDFAVWWDLINCMQEVKNEKEINETYLRCLERSKPKPVREKNDEWRNXIWLWIKYLVFLETSTKYKALCRSKFEELLFDILVPLKNFTFSKVWILYAKHLIRNSSTSIFENLGWKSVLYQSIHLFPKKATFKGFIKLLLEMKDFDSARDVYDKYLQKFPMVVDVWTGYIGLEDNLGDEDRCRALYDACIKTLNDPERKIMYEKYIEFETEQSEYANGRALWSSLCKTIYPDDTMLWVKWSTWELSSPTEEQLEKMNELQEDESEEFAFELTEKNKQNGRNILNSALLHFKTQPLKRLQILESFKKFEEMYGDYKTLEAVNKRMPEIKRTMVRENNVDKEMVEYVFPDDIETENAGLSKLLSMAQKWEEDQTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVM7</accession>
    <name>A0A1E5RVM7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein STB3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g291</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVM7"/>
    <dbReference type="InParanoid" id="A0A1E5RVM7"/>
    <dbReference type="OrthoDB" id="3972639at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000432">
        <property type="term" value="P:positive regulation of transcription from RNA polymerase II promoter by glucose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018818">
        <property type="entry name" value="Stb3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28164">
        <property type="entry name" value="PROTEIN STB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28164:SF1">
        <property type="entry name" value="PROTEIN STB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10330">
        <property type="entry name" value="Stb3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="403"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="544"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="170"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="221"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="346"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="549"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="560"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="625" mass="68199" checksum="ADCC9438FA3F4F36" modified="2017-01-18" version="1">MENHIDNDTTTVNTGNTVNTVNTGNTGNNGDNHGVGHANDHNDVNKSHSNNRTHNIISNNNDVTSRIYPSMLSELLLAKGPLAIRYITTGLDAKVPGFKKLSASKQRRVIMSLLEKGDELNYIVFEKIGWGQWCAVELKDLCNGDLNVFNKKLIEMNLKNEKLNMDLKNTNEVNTNEVNTSKASNSGVSNGGMSNSGVSNSGMSNSGVSNGGISNGGMSNGGVSNNGISNSGXSNKASGSRVNSISDGIVSHDSVNSNPKSKSRKSSPESSGSDTSFGNRSLKLSNILNPLGKHHISEDVFDEDSEMDDSDMEDTFNLNYSFDQKRRKSSVLLSERPLLDSHASFSNNNNNNNNNNNNNNNNTNNNSNSYLTKSSSHSRSGSMSNSIQPIMPLNSKVIKLLHRRSSGGSSTRERDINRSSVGEQPYSNASNIKNYTGSRKNSTQSNGXQGHASLDDFREMVNKTSAAQAWKKGNTTGVITGGATTNTVSANSQIPVTTSNTNNSYNVYTSAFXTENNFQNESFIRSTVSNVKYLDPNGIYEENDWGMGKNKESKENEELKQEEEDNTDEEDWAALGANKLRQRTVLNLNTTEKMDTKNNNAXNIEINETPVDKDAAYLLMNLRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVR1</accession>
    <name>A0A1E5RVR1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Monothiol glutaredoxin-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g409</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Monothiol glutaredoxin involved in the biogenesis of iron-sulfur clusters. Binds one iron-sulfur cluster per dimer. The iron-sulfur cluster is bound between subunits, and is complexed by a bound glutathione and a cysteine residue from each subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. Heterodimer with FRA2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family. Monothiol subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVR1">
        <property type="interactions" value="885"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVR1"/>
    <dbReference type="InParanoid" id="A0A1E5RVR1"/>
    <dbReference type="OrthoDB" id="415696at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990229">
        <property type="term" value="C:iron-sulfur cluster assembly complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015036">
        <property type="term" value="F:disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03028">
        <property type="entry name" value="GRX_PICOT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02984">
        <property type="entry name" value="TRX_PICOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000012">
        <property type="entry name" value="Monothiol glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000092">
        <property type="entry name" value="Monothiol glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033658">
        <property type="entry name" value="GRX_PICOT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004480">
        <property type="entry name" value="Monothiol_GRX-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293">
        <property type="entry name" value="GLUTAREDOXIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293:SF73">
        <property type="entry name" value="GLUTAREDOXIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="10">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="115"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="129"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="143"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ90793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="260" mass="29449" checksum="74A6504783D24868" modified="2017-01-18" version="1">MPYVNISSQDQFTTLTTTEAGDKLIALYFFASWAEPCKIINEVFKVASEEPENKDILFLSINADDFSDIAELFEVSSVPSFVFIQNATILKELSSSDPKEFVKVLKALNQKSTATANSSSTSAAQEGSETAKKLKEDVHEENHEDDDEEEEEEEETEEQLNERLKQLTNAXPVMLFMKGTPSEPKCGFSRQMVGILREYQVRFGFFDILKDDAVRQGLKKFSDWPTFPQLYMNGELQGGLDIIKESLEEDPEFFQHALEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVS2</accession>
    <name>A0A1E5RVS2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Branchpoint-bridging protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g346</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Necessary for the splicing of pre-mRNA. Has a role in the recognition of the branch site (5'-UACUAAC-3'), the pyrimidine tract and the 3'-splice site at the 3'-end of introns.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the BBP/SF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVS2">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVS2"/>
    <dbReference type="InParanoid" id="A0A1E5RVS2"/>
    <dbReference type="OrthoDB" id="6777263at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045131">
        <property type="term" value="F:pre-mRNA branch point binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048024">
        <property type="term" value="P:regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045071">
        <property type="entry name" value="BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055256">
        <property type="entry name" value="KH_1_KHDC4/BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032570">
        <property type="entry name" value="SF1-HH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047086">
        <property type="entry name" value="SF1-HH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208">
        <property type="entry name" value="RNA-BINDING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208:SF45">
        <property type="entry name" value="SPLICING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22675">
        <property type="entry name" value="KH-I_KHDC4-BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16275">
        <property type="entry name" value="SF1-HH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 13" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9 13" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="13" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="7 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="15">
        <location>
            <begin position="284"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="322"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="334"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="376"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="411"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ90984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="443" mass="49556" checksum="60B7C46C0BFC0072" modified="2017-01-18" version="1">MSQGSVSEDGFWTRNNDTSEMDSRTRLKRTVDLPVKIMSTTLTPEQATTYQMLYRIEELSKILNENNYEYAIFPYRERSPSPPPIYDAKGKRTNSREMRYRKKIEDERHRLIDLALKLIPHYRAPEGYSKPTNFRDKFFIPVNEYTHINFVGLLLGPRGNTLKHLQQSTGCKIAIRGRGSVKEGKTSADMPKDAMNLSEPLHCVIIADSEEKLKRGLKACEAVVIKAVTSPEGQNDLKRGQLRELAELNGTLREDTRPCAICGLVGHKRFDCPKREIYADKIICEKCGKPGHVAQDCLVINKYQDQEEXVIANRKRGIADVQEEERGTSAQNDFSDKKIKKADSKHEPLVQSLQSTASPTDKHQPYSSLPPGLEANPQQDTDSFTQTSAGPPGMMDAYEAPGDSNTLTSTLPPPPPPPPALESESQNGEDKQSNEDLPPPPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVS7</accession>
    <name>A0A1E5RVS7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Mitochondrial inner membrane protein OXA1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g443</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the OXA1/ALB3/YidC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVS7"/>
    <dbReference type="FunCoup" id="A0A1E5RVS7">
        <property type="interactions" value="613"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVS7"/>
    <dbReference type="InParanoid" id="A0A1E5RVS7"/>
    <dbReference type="OrthoDB" id="2148490at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20069">
        <property type="entry name" value="5TM_Oxa1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="YidC/Oxa/ALB_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03592">
        <property type="entry name" value="yidC_oxa1_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428:SF66">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEIN OXA1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="OXA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="313"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane insertase YidC/Oxa/ALB C-terminal" evidence="12">
        <location>
            <begin position="142"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="370"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ90823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="407" mass="45246" checksum="5E3687778FC48887" modified="2017-01-18" version="1">MVLKHTVFGLSLKASASRAMMFKNFSSTARQSVTSQAAKLSNGKTPLMSISMTWKRNNSTSSTASESAGSTTSEISSQLQSFSESASTAASPIVDAANQIVGEASSHYGYLESIGLANGWLWPPNLIQHILEVVHVSTGMPWYATIITCALTLRLALIPLYVKQQDNMAKSANIQTKLKHIEDTMYDKDQAHYAKSAAEIKNVKKDIGLSVLWTTVPTLLQVPLALGFFSGLREMCNYPVDGFTNQGALWFQDLSAADPYLGLQCATAAAFIIYTRWGQDPATKKLMNPTYQKVMYVLPLLSIPLTMNLGSGVVLYFAINGLCAIFQGYLLKNPKFRKYYNITEVIKTNEKPLNMSQSFERYKLNAKKRQEFQDRQNSAADKFKKRRQVSRSNSAPPVVEKNSKKYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RW18</accession>
    <name>A0A1E5RW18_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g289</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RW18">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RW18"/>
    <dbReference type="InParanoid" id="A0A1E5RW18"/>
    <dbReference type="OrthoDB" id="435607at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040254">
        <property type="entry name" value="Ecm3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_transp_PIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31274:SF1">
        <property type="entry name" value="AGL149CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31274">
        <property type="entry name" value="PROTEIN ECM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="484"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="525"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="557"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="590"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="632"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="235"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="291"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="235"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="247"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="293"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="306"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="330"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ91029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="656" mass="74105" checksum="B0B84E47800147F1" modified="2017-01-18" version="1">MSVSLGECIYIALKPIXKIYLIMGTGFLLVKTDNLSLETTRGMSNLIINAILPCLAFNKIVANISDRDIKTIGVIVLSCVILFTLGFAGAFAANYVTPVPKKWYYGILFTGIFPNISDLPIAYMQSLGTTGLFSTAEVEKGVAYACVFMSVQNFMLMNLGMFRMIGFDIDVTDEAQEGEEVDLEDKNNSIDREHFQLAKNNFEQTRSLEELVGEHYNIGDLTTKKSMDLGDQSLEQKGLNKSSDTYTKTHKDELTKGKDSQDHAEEEEIEMESLDSLKSYTHNDVYHVQSFELKTEPQKTEKIRRNSNVQYQPNSNCESFQVVRKRSRRNGSISSSANAQSLNGDKPYLSPLGRKKSLIDEYSEQDRISRGDLDLTKPMQLTGDVPIANRGVFLQERVLVDPVSGENRIYTIASKRSLAKICVEEKKDDLESGSCDIDMQSLSNDSEKSKSKIDRLENWFARHKMNWIWYVLVNCQRPASVGTILGFVVAMIPWVKALFVHTYVHVHQAPDKQPVLNFIMDFTSYVAQACVPLGLLMLGATIGRLEVKNLQPGFWKSAMWMTFVRLILMPIIGILWTNRIYNIDWIDTRIAKFVIILTFAMPSATAQVYFTAFFSPIEGDHLQMDCLSVCILMQYSVLFVSLAFVLSYALKVDLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZC2</accession>
    <name>A0A1E5RZC2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial import inner membrane translocase subunit TIM54</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g24</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TIM54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZC2">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZC2"/>
    <dbReference type="InParanoid" id="A0A1E5RZC2"/>
    <dbReference type="OrthoDB" id="5598305at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021056">
        <property type="entry name" value="Mt_import_IM_translocase_Tim54"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11711">
        <property type="entry name" value="Tim54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ92322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="485" mass="55538" checksum="B1FD84C362DA3635" modified="2017-01-18" version="1">MSTEPKPSASGVGTPASKPIPDVANSTPSPKPKKNPAFAAMGIPSFKAKLPGPKWMAFWCVVAAGIGGVVYDKREQNKIRDKYCELVKQRLQESSTNNDYPWYMKSRKISVFIAPPPNDYLETSMKTWRRFIKPVLYSSGIDYELFTTDTQGALRLQVSREIRNLRKSILEHEAELEKLXQEQKLHASYKYKVNHAWKEFKNWNTTNIWNXITKKNSAGEQEVVDPVEEMIQAKKFKAEFDLKNLLGVFYDNEDMVKHAKSVTEDSLQGDAALAGGVLCLGRGAYKEYLAGINEGLLGPLEMPESVKADLEAKKEEWRELQRKNFPDQLLDETKMPDYVPKSFLENNDTHAFTYPPPEVNDKVRDSETNIPYFFKQPLICIPQPIISGLVNFPRKVYRFYTKRYLVEEYCRYATSVVLQDVRPFAQEDLLMGKTEEEDEWPSGWVKTGVSKNSEWVQPAACDSRILKDLLTYNTDSVPYLYSSFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZC6</accession>
    <name>A0A1E5RZC6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Structural protein MDM1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g265</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000002">
        <property type="protein sequence ID" value="OEJ92149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZC6">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZC6"/>
    <dbReference type="InParanoid" id="A0A1E5RZC6"/>
    <dbReference type="OrthoDB" id="120967at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003114">
        <property type="entry name" value="Phox_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775:SF3">
        <property type="entry name" value="SORTING NEXIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02194">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00313">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51207">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PXA" evidence="3">
        <location>
            <begin position="38"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="1">
        <location>
            <begin position="403"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="742"/>
            <end position="859"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ92149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1037" mass="118852" checksum="562DF437A07C65BE" modified="2017-01-18" version="1">MFSAPEIPTPHPPTFSNVNEEEIDIAHLEAENYISKHYPDFNDKIRSIVDALIDKFVFSWFENIDKNKDSDFIKQVRFTLNECIDNVVIQLENKADSENELVSKVLSKILNLTQKHFSAYISASSMVTTQAPNKELQLAIXFHKQFKLHKAVTLTSSIPEFDREVQNYLRETVEKVILPLIMAKDELQXSVGTILIREVLVCTVFKSLLDKFSSLDYLNRKXIEVSENLMKEQQQIRSLRKFLSAKISRGNSVTKNKVGSLENSKCSSLEIDEIIAHYGNNEQELIGYGQNMITQLDINCSGKQFESLLKQITALNHLAMLKYLRNLIILQLINFSSTSELKKKYSRKSISVFRERLELLLDLIEKKVPTDYDSTIFKFSEENLHVQSNVLRRKFESRLATLNLANVLSDPTLRQHFENFVSDSNPEISPLIEFWKSVEEIKSPLESSEVQVDVGCDLLGAQQKDDIRNICIKHLIQMKKLFENNPDYELLQEYYNMKTSISYVAARQALLLLQEETLRYLQTKLFPMFLHSKEACFLLAKIEDSTMQVESLVSHSNVQDILTEKLENGAECDVQQKSTFENKRIQSSSSMFYSNDISEALDNILNEKANKSSNNDYLFGNPAEGIFKQDSLFADEEDDDEAQAASKITTAVXLAEQNNMSVSDHLPSTIDTSACPPLSIELLTMSIHDLDKQLSLLRHLILKAELTNNQKELRLLKKSERIVLRELAQKKNLKKELGLNELFLFNNTDIAISNYIVKDDISYYNVSVTYLAHTSEMQAWSELKRFSEFVRLNAYMRKTYEKTKVLKFPAKLELAFNKITMLQERKIKLEKYLNDLISIPQVCQDPFFVKFLTTSSYPNGGYYKPDSSSLRSSSSEYYDTESKESAADLTANLTLVGDVANESIFIKPMCDIFSSMFQLEQGTSVRGKTILVILQQLLGGTVERYIKDMIKRTTSKEKLLEILTXAGGFMKNDTSLEEMALRENVSEQTARMNTLFGSDIRQDQSKEHFSKPPNEEDPEFLKRKSLLSLESLLVYQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZM9</accession>
    <name>A0A1E5RZM9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Lysophospholipase</fullName>
            <ecNumber evidence="6">3.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g164</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the lysophospholipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZM9">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZM9"/>
    <dbReference type="InParanoid" id="A0A1E5RZM9"/>
    <dbReference type="OrthoDB" id="4084751at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF56">
        <property type="entry name" value="MEIOTIC PHOSPHOLIPASE SPO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5 6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4 5 6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLA2c" evidence="7">
        <location>
            <begin position="27"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ92432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="681" mass="77334" checksum="27FE09BE614A2BE3" modified="2017-01-18" version="1">MVLCETETQKVSGTGENICXYAPTRVVCPERSLIRSGEIGLCNAETDYMNKRYSLHILKNLMTFLQKCDISGLNVSKYIHEVNTVPPAIGISLSGGGYRSLLLSAGVMQQMDKLELLPCINYVTSVSGGTWLLAKLILEEFEDLQLATWDFRRSLLEGVSNFELSDEDVGDTDLISYKPWLNDNDSEHSSNLNTALQKRGFSNQYKILKIKLDSFINSFTFANHENSTINGSVTDMVYFMNFLDDLTTLQKTLHFYESIHAELKEKRLLGFPLSFTDYWGRALSKRMRRSLNKFDGSEEHXXSSVGKXHELTVRKSKKFKEAGMPFPIIVANCKNXPQMSNVVFEFSPFEFGSFHRSLNKFVEIEYLGSKIENGKSFECFTGFDDLGFISATSSSLFNNVLAVVWEMVHNCSKETFQAMSTILGSFGLNVEHAHLSSNEKPSDTKMKPDYAVFHPNPFYVSNDTESKDNSLTGKNYLYLVDGGEDGENVPLRPLLRAERRMDIIFAVDSSSDAKSWPNGKNMYNLFVNMQAENNTRTIKWKNKVIDVLNIPRIPKSITDRSKPMVFGCTVEKYIYSIKNKDVENKSTSKFLPPMIVYIPNFAHVFPSNTSTFKTQYNETEVRKMMQNGRAMLLSETADLQEAAHFQKCLACLIIKRSFDNGIAFGNPPIFCNMCYKEYCYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZQ9</accession>
    <name>A0A1E5RZQ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cytochrome c oxidase assembly factor 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g46</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for assembly of cytochrome c oxidase (complex IV).</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of 250-400 kDa complexes called cytochrome oxidase assembly intermediates or COA complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the COA3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZQ9"/>
    <dbReference type="FunCoup" id="A0A1E5RZQ9">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZQ9"/>
    <dbReference type="InParanoid" id="A0A1E5RZQ9"/>
    <dbReference type="OrthoDB" id="10018333at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041752">
        <property type="entry name" value="Coa3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15642:SF3">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 3 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15642">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ92462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="88" mass="10307" checksum="903CFB53E002F8BB" modified="2017-01-18" version="1">MAFEQSRYQDPVTYKMTPAMIRARRPFFAKNMFGLGILLGVTAGVYGYTYNFLHRDGDFSDVPIPPIDAKELEQLKKEYEEHKKQRDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZU2</accession>
    <name>A0A1E5RZU2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing factor ISY1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g200</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ISY1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZU2"/>
    <dbReference type="FunCoup" id="A0A1E5RZU2">
        <property type="interactions" value="240"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZU2"/>
    <dbReference type="InParanoid" id="A0A1E5RZU2"/>
    <dbReference type="OrthoDB" id="1739576at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009360">
        <property type="entry name" value="Isy1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037200">
        <property type="entry name" value="Isy1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13021">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ISY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06246">
        <property type="entry name" value="Isy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140102">
        <property type="entry name" value="ISY1 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="7">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ92356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="250" mass="29358" checksum="C5CEB35A623432BB" modified="2017-01-18" version="1">MSGGSNDILARIQEIEAEEAENSQYKNYSRYARPTNVRKVTSLQECYNWRKQIIKDCDQTYTKLFETTLSEDDVRNYNDLYNQYMDELNKWDRHIVQDLNGHSFKNKHVTTTARQSRLLKRNSAKSESVDRKSMKKINGKWYVGKALELPEIKDELLGIEALQNQKRAHLNNEAFLKRLNKSLNRKEYFKGAPAGLAVPSDLLDQVALSKEEVEKLKEERRIPDFVIPTMKDMEQWLVNKRKEKLKSVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZZ4</accession>
    <name>A0A1E5RZZ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alpha N-terminal protein methyltransferase 1</fullName>
            <ecNumber evidence="5">2.1.1.244</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">X-Pro-Lys N-terminal protein methyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g60</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-terminal L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N-terminal N,N,N-trimethyl-L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54712"/>
            <dbReference type="Rhea" id="RHEA-COMP:13785"/>
            <dbReference type="Rhea" id="RHEA-COMP:13971"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138057"/>
            <dbReference type="ChEBI" id="CHEBI:138315"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N-terminal L-prolyl-L-prolyl-L-lysyl-[protein] + 2 S-adenosyl-L-methionine = N-terminal N,N-dimethyl-L-prolyl-L-prolyl-L-lysyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54736"/>
            <dbReference type="Rhea" id="RHEA-COMP:13787"/>
            <dbReference type="Rhea" id="RHEA-COMP:13974"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138059"/>
            <dbReference type="ChEBI" id="CHEBI:138318"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N-terminal L-seryl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N-terminal N,N,N-trimethyl-L-seryl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54724"/>
            <dbReference type="Rhea" id="RHEA-COMP:13789"/>
            <dbReference type="Rhea" id="RHEA-COMP:13973"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138061"/>
            <dbReference type="ChEBI" id="CHEBI:138317"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. NTM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.244" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZZ4">
        <property type="interactions" value="465"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZZ4"/>
    <dbReference type="InParanoid" id="A0A1E5RZZ4"/>
    <dbReference type="OrthoDB" id="1298661at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071885">
        <property type="term" value="F:N-terminal protein N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008576">
        <property type="entry name" value="MeTrfase_NTM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12753">
        <property type="entry name" value="AD-003 - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12753:SF0">
        <property type="entry name" value="ALPHA N-TERMINAL PROTEIN METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05891">
        <property type="entry name" value="Methyltransf_PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016958">
        <property type="entry name" value="DUF858_MeTrfase_lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="189"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="128"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR016958-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ92511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="256" mass="29029" checksum="2DDDCD42A0F0E4E9" modified="2017-01-18" version="1">MADRNINYKDAVDYWESVESSVTGVLGGYGPETSLPAVEQLGSLKFVRKLKSRMVISSADYEQYGKRCIDFGAGIGRVTKNVIYKVDPKSITNLDLLEPAKNFVDQISVELEEIQKEGKLGNVYHMGMQDWPPENVDKTNIKYWLIWCQWCLGHLPDNELIEFLIRCKKLLVTNGTIIVKENNTQSASYLGTGENKSSTDNDDFDPQDSSVTRSDSKFRWIFEKAGLKLIATDIQKGLPKELYPVRMYALKAXDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S0D2</accession>
    <name>A0A1E5S0D2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cullin family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g62</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cullin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5S0D2"/>
    <dbReference type="OrthoDB" id="5581181at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cullin family profile" evidence="2">
        <location>
            <begin position="597"/>
            <end position="670"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ92509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="737" mass="85814" checksum="6EA0D74929F7EC7C" modified="2017-01-18" version="1">MDLLAISLEAVSQRVKACIEEFMTTPNVVKARVLDHTVEKAMSMKVFEKADTMNTDNDAVVIHDKLVLKNLRTIIXTSIVDKYTKELQNCKDITEVVSLCSSTFFPSYQNIISEGQDQCLHATLAIFYQHSAPHFKLKSLTFSMFFELLIDAITLTDSLFSQLMHHLFVGFLENKDQKTVIFQHVLLFFYYNKPSYQINLLCRNYFEEYADTNVLGDLLCKSSGEMIATLKSGSFQTEQIDEHFRCFEKYYLLCSKIGGPLAXSERHWFIHTFMKQRCRNFVKLIWFVIAKDGSRSRNXVKXSGVYEEPLNLRFWKDQVTVSIILKYYHGKFLNGFGEAWLEFFDLKNVNPANFMDTSMKILVDIRADQNPYLETFHSDQYSVLTEQLTNTLKYNEIDSSKAKIKEQLTTIFLETLFDLCNGRENFYKKFLKYCHSNQSEKLCKDNYTLLLSHFAEIAXHLGDPFYTFYYENYLFKLAIRDNNPDILKIQSEGPFSVRNESTENDFTLFVLLTISIKAQFPLNERHARYLEKCDDFQNWALSHDNRVLCLNXHRDTFPKSILQNHDENLEKCLPPEHPVTLLTEIANKAWSSQYNAQNKRSVTKMYELSHGVIGTPYRLPNTEFLNLHVNFFQYMILDCYQEQDELSLEDLLQKTNLQLKDVVPALQSFMKINMVKQTGRDKKTFFALNLEPGTLKISMQFLQKGNVLKLPYRVQLIVSRGASTGDATNKSKRLKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R008</accession>
    <name>A0A1E5R008_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Pre-mRNA-splicing factor SYF1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g4655</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the crooked-neck family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000017">
        <property type="protein sequence ID" value="OEJ80240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R008">
        <property type="interactions" value="1083"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R008"/>
    <dbReference type="InParanoid" id="A0A1E5R008"/>
    <dbReference type="OrthoDB" id="10067343at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055433">
        <property type="entry name" value="HAT_Syf1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055430">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045075">
        <property type="entry name" value="Syf1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246:SF5">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR SYF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23231">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23233">
        <property type="entry name" value="HAT_Syf1_CNRKL1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1-like N-terminal HAT-repeats" evidence="9">
        <location>
            <begin position="12"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1/CRNKL1-like C-terminal HAT-repeats" evidence="8">
        <location>
            <begin position="430"/>
            <end position="803"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="838" mass="98167" checksum="CF22F5BE167762B9" modified="2017-01-18" version="1">MELANKYIQSSEEIAYEYDILKDPKNAVTWQRYIDHIKGQPNDNETYKLEKLCFCFERALQELPKDENLWLSYIYLLDEINAMEKYISISKKGLEHCPTSYKLWTAYLSNLFELRKLDQFGQEFTKCLKNLPLAEHVSLWNETVLAYISDMYPTEADENTEDSVWNGFLLQPYFQKQLQSQINSMVGESPDTCTSKHENDSWSSFYLTKYAHLMALRQDSEAQLLXIYXILCKTQNYXELSIFLEKFVFPKKKTSDLYFQYLSCLIYNPXLSSDXDLENMIIEAMKTYPTDKKYFMLLLCHEKVMRKTTGLSEFYEFMNTLLNTTTDAPTFNLLYEFELNFEENALALCSKDESRLLHLEKLEGFTNNYSLMANTLQIRKXPNRISLWKQRLKLGKKNDQNFLISVYNEALENISPVSYELEESRSTMGKGQLGEFWVSYADVYYRAGDYDNFRQVMNTAKKVPFKYIQDLEHIFTYWSECEMALNDAEQAIAVLKTALNYPEKFSTFVENANLVLNNYYSTQEFVPAQVILPLKSFXLWDKLLQIYELYKPLEETNALYEAVIAIKVAKPSTFVTFAALNFNNTSKWIAIYNKAVMTFRSSKSVLLELYEIFLPLLQQRLHPXTFVRDCETIRDCYECLLXQLEEHKVDCVDYYLRYAFFESSIGNYXKSIGILYRGTTSKNVDIMKSKWKIWEQLLAMAEENLGIEQVRAYFKESLXTLPNSVLDSVVVRYAEYEVHALHQYDRARAILQFGAECGPVQQHASVWDYWKRFELKYGNKETFKNMLRMKRIVGDKFVVDTQAFGKLDNNVEFVSSGVQXGLAAPQDENDTMEIELDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0C8</accession>
    <name>A0A1E5R0C8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Nucleoporin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4411</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0C8">
        <property type="interactions" value="110"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0C8"/>
    <dbReference type="InParanoid" id="A0A1E5R0C8"/>
    <dbReference type="OrthoDB" id="5529162at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070762">
        <property type="term" value="C:nuclear pore transmembrane ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056541">
        <property type="entry name" value="Ig-like_POM152"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056542">
        <property type="entry name" value="Ig-like_POM152_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056543">
        <property type="entry name" value="Ig-like_POM152_9th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056544">
        <property type="entry name" value="Ig_POM152"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037701">
        <property type="entry name" value="Pom152"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056540">
        <property type="entry name" value="TMD_POM152"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28206">
        <property type="entry name" value="NUCLEOPORIN POM152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28206:SF1">
        <property type="entry name" value="NUCLEOPORIN POM152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24312">
        <property type="entry name" value="Ig-like_POM152"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24519">
        <property type="entry name" value="Ig-like_Pom152_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24527">
        <property type="entry name" value="Ig-like_Pom152_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23664">
        <property type="entry name" value="Ig_Pom152"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24097">
        <property type="entry name" value="TMD_POM152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="176"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 N-terminal transmembrane" evidence="4">
        <location>
            <begin position="108"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 first Ig-like" evidence="6">
        <location>
            <begin position="248"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 Ig-like" evidence="5">
        <location>
            <begin position="565"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 immunoglobulin-like" evidence="3">
        <location>
            <begin position="632"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 Ig-like" evidence="5">
        <location>
            <begin position="854"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 immunoglobulin-like" evidence="3">
        <location>
            <begin position="982"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 ninth Ig-like" evidence="7">
        <location>
            <begin position="1190"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1379" mass="156604" checksum="553FAAA110D97D3B" modified="2017-01-18" version="1">MNYSNNVFDSPGGAKSPFGSRNWNDTPLRKRKDIDGFTRPRGSLKHEYTPTFDSFGPTTPNAKNEGLFASSPNSKTTKQGNNDEQHHSPCKQKKKYNTLISTDVLSVGSQRGLALSIGALIWLYKLYDLIDMKINLPKKDYEFSRLSFIVKFFILDFLYLYSLPVFKIPKLNFKPFVTYFFIVLWVAIDVLISSNSILPFLTLLVSTYSSLNPQKMAISGNTVNYKKITDSSSHFKGKHIIKILPENTALFNPFHDSYCFSEQYGVHNVQVPIRVNSTIDIGFLQFEHRDLYTNKPNLINISTSIKNEAHLIKKIVKHDERLRFLNQDSISNIEKAQSIQYYNVTLSKPGYYQLVKLLDKDNFQLKLYRSNLVVPECPRASFKPVEKNSLEKPSTNLYYDRCLGTEDKISITVNGVPPLQLQYGKRSGQNSIKETVIDDTSLQPPNFLNGDFSPLMAQHAVNKLSKNDISNLKWAQNYDVDIELQSVSKDQGLHGYHIYKIIDGFGNVIQRDILFKNDKNNLDYEYYIHGEPKASLSERINAKSPTKKSLVITLEDFDAQKKLYQHDGPFDVEILYKREGSDDVEIIHQSLQKNKHLEIIAEKSGEYVLNSVNSKFCPGIVLGENRVVVTEPVPPQMKIEATSIQDQCAGPIGLDFNLIFTGVAPFEFTTKIHKINPITGQKTLYESKKRRSMGTRHQFLFNPSQVGDYEIEFVDISNTVFNTPIKLQPLSEYLFKTSMKVKPDAKITNNNSNNNRFNGNFVEKLCLNESRSVSVDLRGEPPFELKYDITETNTNERTFFVAENVMENHLQLATPNFAKGGNYVFSLASVKDSTGCLVPLSGEEVSYNVRRLAPSAEFNFIDEDHQHVIIKAGSSTTLPLRLLGDPPFQIGYQHASPNDKFGAEIKYATITSKVRPSLTLKEQGTYKLVSIQDNACKGFVGSQNSFQISYLPQPKLKVLDHNHGKINKINEFKFLKNDVCEKVEQVIELQIDGLAPLNVTYDVIIPNGHVLKNQYLQVLTNYATIKLPNEKSGEYKIVIKNVNDANYPDTVNMKPIEVIQNVFKKPDLRIFNKNGAKSFRTCYSNIDEPSAYLEKIQLQTVTGNKVGLYAVTFKLFHETSGKTDYFVLETSPKGHGEHGNVLDIDYKQIYGKLKMGTYSLTIDKIVDSVTGCVGKDSSLSSFSITITDVPTISLLEPSLDYCVGDYVAYQLNGQLPFEIKYAFNKKKLKTTEHSSQFIRLASEAGEISINSIQDTQAKCLVNFNKPGMERQFRDLSLNIHGKPTVTTSKGDVFEEDIHEGEQAEIIFTFEGEMPFQLKYVRMENEITSFKDMKNNNVEIFTVSDIYSYEYRFVTSLQGTYEAIEISDKYCTARNEEFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0G2</accession>
    <name>A0A1E5R0G2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nuclear cap-binding protein complex subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4407</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0G2"/>
    <dbReference type="FunCoup" id="A0A1E5R0G2">
        <property type="interactions" value="1005"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0G2"/>
    <dbReference type="InParanoid" id="A0A1E5R0G2"/>
    <dbReference type="OrthoDB" id="10252707at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005846">
        <property type="term" value="C:nuclear cap binding complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000339">
        <property type="term" value="F:RNA cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027159">
        <property type="entry name" value="CBP80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015172">
        <property type="entry name" value="MIF4G-like_typ-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015174">
        <property type="entry name" value="MIF4G-like_typ-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12412">
        <property type="entry name" value="CAP BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12412:SF2">
        <property type="entry name" value="NUCLEAR CAP-BINDING PROTEIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09088">
        <property type="entry name" value="MIF4G_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09090">
        <property type="entry name" value="MIF4G_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIF4G-like type 1" evidence="1">
        <location>
            <begin position="359"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="MIF4G-like type 2" evidence="2">
        <location>
            <begin position="604"/>
            <end position="853"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="888" mass="102271" checksum="C5442759EBD6C8F1" modified="2017-01-18" version="1">MRASYYEDDHEQTNEYQREFRRPRTFKRARLPPGVMLAKQMLPDICTIGEKPEILDEDIDLISEGIVQNFEVEEYFRSNLKLVLWAIITEQPHKQPTIAILLMKVYSLDAAVGKTLVNFLHQQFQDSINKTVSNDTEVAQPSEYTGFWNKAKLGLRFFSLLTPIISEDDILSLYTGFFDLATQLNNTGSEKRVPLAELIYFNTLLAVPQLFLFNPVSSSTLYSKVQNILSTVEQSFKVQVTEPLDLNNEFNNGNQVYEKVNIAQVIMKAVQGVLANDLAGIKDLYPDYSHLLTFLKDTNTEQSQGFNDPLIFPTIDSLQKNIQLSDEKASGSVDGLWKYPRYTFELYQTNAIGEFDTVPERTSFAGLLFHDILVDVIQSLEFNKDTVSEQVVLINMYFKQGFFAPKGLSISQLIDLKENDPNASTLKLEDLAVETILSLVFKLPSHADFFYMYYYTLLVSICTASARSIAPVFGRAFRFYYSHLNVLDSELRIRFLDWFSFQMNNFNFSWKWNEWELDSQLYGNKKTFYNPKINFIKNLIKKELRLTSSPQEISDSLNDEFLQYTNCSLVSKAELKNYYETFLKDVEIDDQLFESQSAVFVLLNEKLPFSKETQSVVNYFRKKERTIQELHGIIGKLESEYGQYISNVDKLIVTLLTQAVIHAGSRSISHASNCVRDFKEDLAEVFGVVPNAQEKDKWVIEAIMRYWNFDSKTGLTIVSYFFKNNLISAKNSLVDFLFNEYETNQSIGLVDSTFIESLLDLLQNEETETDLSLYQYVFEKISLLANDSISKLNLSASESLPSIPNFDYYDFEDPETPKPDISAEDLAKYDLVWKMESSVSLIKSIIRKYGKKYSLLAAFFKESINETTIPYDPIRNQLLKYVDEASQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0K8</accession>
    <name>A0A1E5R0K8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Protein ERP3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g4460</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0K8">
        <property type="interactions" value="74"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0K8"/>
    <dbReference type="InParanoid" id="A0A1E5R0K8"/>
    <dbReference type="OrthoDB" id="1929172at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184570" evidence="10">
        <location>
            <begin position="24"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="11">
        <location>
            <begin position="33"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ80439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="221" mass="25730" checksum="565D00C41083EA89" modified="2017-01-18" version="1">MLQRGSFFQLAILFCYTALLANANPITFKVSQKECVHINTPEEGCRVVYYFAVQSGDKNDYEINYWIHGPESEEALASRQNERQGEWEFVAALGGEYSFCFEPSTSNSKRTIDFDVKYTCPNYIGDSKFTESFLKDASKHYNEEDTKMDDFLDTLDVKFNTLEKNLEYYKTRNNRNEQTVESTERXIFWFSLSALALTGLITVGQSYLLKYVFQRSRRNKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0M4</accession>
    <name>A0A1E5R0M4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4404</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0M4"/>
    <dbReference type="InParanoid" id="A0A1E5R0M4"/>
    <dbReference type="OrthoDB" id="3026777at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06174">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507:SF1">
        <property type="entry name" value="FI18259P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507">
        <property type="entry name" value="ZGC:174356"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="290"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="383"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="423"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="465"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="489"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="559"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="53"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="691" mass="77411" checksum="460F8B9C59841F93" modified="2017-01-18" version="1">MTSEELNSNTTSKTQDNALGPAVQLTDEELSANPEYVLEQTLSTILSRPVVCDEESDVDQETNALFTDDDDGDNADGNQRTSNENNRHKQSDSPQTGLSRWKPTLHKPAPSIYILCSLMSVKVLAENLVLGVITDLTLAKIQKYYEDNERQSQKEFAKFQSVTSVIQGLLGISLCSKYGHLSDNHGRVFVLQICGLLTILGSLANLYIYKDSTTYEFYSFVFFYCIEALNAGQMSMVAVGNSYVSDIVSEDNRMVCISFFMSIMYATIGIGPLVGSFLVKIFKGNSFNTLYVALFLNVSYTLGVEFFLSESRTVNERRESQSQYHNEVLRKNLRKEQAIFSKESSKWSKVYHLLKMDTLIDLFKPLKTLWVPKTALGSLVPRINVLILVIIEMFVGGSVEGSVQAIVTYSMFKYSWSSVEIGYYYSISGVGRALVLLLAVPQLISFLKRRNYVALPDSIDRIDMIHLYLFLIFVITAFASMIVYETNDIGSYLNSLFISLSAFALPTVQNGVIKYASKKSIGQVFAAMAVLRHLMSLIAPPLYLQVYAATAKTFPTLFLYIPIILSALSLVLLQFVKIVKDHDMLRRQSEASFNNFDEHINNYSVNTKPNTSFDIANNLKNGKSNLQSNSIPIETTPLFKKSHFTGKDNIRNSYKSLRNSFVNSNTPSSKNSSLDKLKAQNFSAFNVPPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0Y2</accession>
    <name>A0A1E5R0Y2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4594</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R0Y2"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018860">
        <property type="entry name" value="APC_suCDC26"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10471">
        <property type="entry name" value="ANAPC_CDC26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="81"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="116"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="163" mass="17983" checksum="6BE7C352D98FCE81" modified="2017-01-18" version="1">MLRLRPTTLQLTQQDVLEMEEQIDNQIRINKQKAQLECASAQTAVFSAKESAPNSGMLFGMGSEPDRKXLFPASAXLPQHSEKIFSGTNSNSKYSNARAEXSLQLHYERIQRSGPSHSSSASSASSASSARSPTQETEPRVSARTDNQVDFITARXDTSERFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0Y9</accession>
    <name>A0A1E5R0Y9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">AP-1-like transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4646</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000017">
        <property type="protein sequence ID" value="OEJ80233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0Y9"/>
    <dbReference type="FunCoup" id="A0A1E5R0Y9">
        <property type="interactions" value="3598"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0Y9"/>
    <dbReference type="InParanoid" id="A0A1E5R0Y9"/>
    <dbReference type="OrthoDB" id="3973091at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.100">
        <property type="entry name" value="YAP1 redox domain. Chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050936">
        <property type="entry name" value="AP-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013910">
        <property type="entry name" value="TF_PAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023167">
        <property type="entry name" value="Yap1_redox_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621:SF6">
        <property type="entry name" value="AP-1-LIKE TRANSCRIPTION FACTOR YAP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08601">
        <property type="entry name" value="PAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111430">
        <property type="entry name" value="YAP1 redox domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="6">
        <location>
            <begin position="39"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="146"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="550"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="157"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="205"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="233"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="254"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="391"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="645" mass="70762" checksum="2E10B5F7409F2CDF" modified="2017-01-18" version="1">MPVVKRHIEDVAGMSPSESLKSGDLIHEKKVGRKPLTDTNVKDKRTQQNRAAQRAFRERKEQKLKELESKVESLETINKQQELEQELLKEQLSVLLKELSKYKQTSSTANTSANKGADFKASSGFASAASAKNIEDAISKHSHFFMQNQGKNGSGNGDNNDNTGSSSNGDLPNTNNSTSDSPLSSSLSFNSMNSTYNRNQERPSFHSSSSAGSDHSFTASKPHTFSFPWKIKSPQSNHNSANGTPSMVDHPAGNSVSSLSSNTSPNDNFDKLEKFNSLLSNHSSLTDLYDIDIPEINSLGKPKDNTLQLTASSSIQKNNNSATSEEQRLVDNDLNTFDHKSNKSLRNKQEQQSLDPNYDFTNSFDEQVFCSNLGKACPYTPGQSTNELLANKSSGKTENPGLSTSTAAAPTEALNKKSPFAFGINGFDPSILAPSFGESGFSPSNLFTSPSSGEINNAESYNLVNENTTMPEHVTNEFGVNDDLVGLNALGNIGNKNGEVKDHFIIDNNIAFDSFDWDNLINNDIALPSAANNNWDTTVKKDISAVNPFELKVRDELDDEEEDINLLKQKIKADEDDNEVVPSTDGDLLNCSEVWDRITTNPKFTNIDIDGLCSELRLKAKCSDKGPVVKEQDMYSLLKQHEIDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0Z0</accession>
    <name>A0A1E5R0Z0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g4590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0Z0"/>
    <dbReference type="InParanoid" id="A0A1E5R0Z0"/>
    <dbReference type="OrthoDB" id="4031940at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ80550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="425" mass="48758" checksum="07B9F2EC18E2F401" modified="2017-01-18" version="1">MSLINEVPLIFQKNNIASYEGHPQGPSLINQRQHIIALLXASVSTGNGLKVIDAKNXNFNLIDTLTFFIESLLNSNVINDDLVVSLLLLSKQYHLQAHEILHQNYGKPAEEDHSWNVASRYFQLSIGVLKFIELQLFPLYTCSAPDLLKVWNVFYHYNVIYSQFSMVILGLYKLKDSLFDNYSQQELNNQTKLQGLSFNMLAKILINIYNTCNNCITNIASLGNSKNSHTSLKNFVIFQQQYIYGLIELFLSLQSFEIDNKIGLSCSYIKHAIGLYSCSKDDKTHSSVFYPFMVEMGNLILKDQKKTHELKSLFKTKYLTQKNKLKIKSQKLAPSIKHHVAFNDSLVTETFQDFIAPLLYLLEYRYNHFNNTIALEPIITDEQELQKNWPVAMGTPLKCITWSFKNGKFASENEGQRSTPTATYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R144</accession>
    <name>A0A1E5R144_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g4278</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="4057220at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1840.10">
        <property type="entry name" value="YNR034W-A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021591">
        <property type="entry name" value="YNR034W-A/EGO2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035098">
        <property type="entry name" value="YNR034W-A/EGO2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11503">
        <property type="entry name" value="YNR034W-A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160683">
        <property type="entry name" value="YNR034W-A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ80629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="89" mass="9825" checksum="1D9A235801CE8C7B" modified="2017-01-18" version="1">MDPSKTLDLTSLIPNAIGHIAFDESNNVIDTSGVGKDRRADVENLSQVELQDGFALLEEKDVKIYIYKQMGKTIAVYAYEGTATTDENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1H2</accession>
    <name>A0A1E5R1H2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Heat shock protein STI1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g4088</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">Part of a larger complex that includes HSP70, HSP90, and immunophilins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1H2">
        <property type="interactions" value="1021"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1H2"/>
    <dbReference type="InParanoid" id="A0A1E5R1H2"/>
    <dbReference type="OrthoDB" id="2423701at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042030">
        <property type="term" value="F:ATPase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.100:FF:000004">
        <property type="entry name" value="Putative stress-induced-phosphoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000010">
        <property type="entry name" value="Stress-induced phosphoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000020">
        <property type="entry name" value="Stress-induced phosphoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.100:FF:000002">
        <property type="entry name" value="Stress-induced-phosphoprotein 1 (Hsp70/Hsp90-organizing)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000027">
        <property type="entry name" value="stress-induced-phosphoprotein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041243">
        <property type="entry name" value="STI1/HOP_DP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006636">
        <property type="entry name" value="STI1_HS-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904:SF523">
        <property type="entry name" value="STRESS-INDUCED-PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904">
        <property type="entry name" value="TPR REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17830">
        <property type="entry name" value="STI1-HOP_DP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00727">
        <property type="entry name" value="STI1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0346">Stress response</keyword>
    <keyword evidence="4 6" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="3"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="72"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="STI1" evidence="8">
        <location>
            <begin position="136"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="327"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="387"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="STI1" evidence="8">
        <location>
            <begin position="525"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="196"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="205"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="9">
        <location>
            <position position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00064323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="576" mass="64727" checksum="2AA313FB428964C3" modified="2017-01-18" version="1">MSVEEYKSQGNEAFKAKDYSKAVELFTKAIEAGDEPNHVLLSNRSACNVLLKNYADSLRDAQECVKLQPSWAKGYSRVAAAQQGLGNLDEAEASYKKCLELDPANAAAKNGLKQVKDQQDQRQSMPDLGIGKMFQDPNLIEKLKKNPKVGHLMNDPALVQKLLQIQANPRAMGPEVIQDPNFMSILAALMGIDLSMDPQESGSTPKDDFVPPKETETEEKASEPAQKEAPKQXEKEKAPEPEAMEVDDEKTKSLELKNEGNKLYKAKQFDEAVAKYEAAWDLYKDVTYLNNKAAAQYEAGQYEDAIKTLNDAVDQGREYRADYKIIAKSFHRIGNCYVKLNDLQNAIQFYQKSLTEHRTPEVLNKLRKAEKDIKIQAEKDYLNPEKAEEARLEGRDFYNKGDWPSAVKSYTEMIKRSPDDPRGYSNRAAVLAKLMSFPECIKDCDIAIEKDPNFVKAYIRKATAQIAVKEFGAAIKTLDAAQEKDVAKAHAREIQQLYMKASQQRFQPENANESPEQVYARAMKDPEVAQILQDPVMQSILQQAQSNPAALQEHLKNPDIMQKIQTLISAGIISTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1N9</accession>
    <name>A0A1E5R1N9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.205</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">2'-O-ribose RNA methyltransferase TRM7 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g4247</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>cytidine(32)/guanosine(34) in tRNA + 2 S-adenosyl-L-methionine = 2'-O-methylcytidine(32)/2'-O-methylguanosine(34) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42396"/>
            <dbReference type="Rhea" id="RHEA-COMP:10246"/>
            <dbReference type="Rhea" id="RHEA-COMP:10247"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74445"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.205"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. TRM7 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.205" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1N9">
        <property type="interactions" value="926"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1N9"/>
    <dbReference type="InParanoid" id="A0A1E5R1N9"/>
    <dbReference type="OrthoDB" id="289250at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106340">
        <property type="term" value="F:tRNA (guanosine(34)-2'-O)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002128">
        <property type="term" value="P:tRNA nucleoside ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000176">
        <property type="entry name" value="tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03162">
        <property type="entry name" value="RNA_methyltr_E_TRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028590">
        <property type="entry name" value="RNA_methyltr_E_TRM7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF12">
        <property type="entry name" value="TRNA (CYTIDINE(32)_GUANOSINE(34)-2'-O)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="8">
        <location>
            <begin position="21"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="308" mass="34658" checksum="BFD27D7B856B0224" modified="2017-01-18" version="1">MGKSGKDKRDLYYRKAKEQGYRARSAFKLLQLDEHFNFLNKDLHKVVDLCAAPGSWSQVLSRKLFEDNDDQCGNLNKKIIAVDLQPMSPISNVITMQADITHPKTLYKIMQHFDNEKADFVCSDGAPDVTGLHDLDXYVQQQLILSALQLTTCILKKGGIFVAKIFRGRDIDLLYSQLGMLFGKVICCKPRSSRGTSLESFIVCLDYNPPQNWTPHLDLNISTQDFFKGITIPREDGGVKNFCELSLDDREISDFISCGGLQSFDSDATYHDLPETSKSLDPVQSPTNPPYKRALELKKQGKLGKVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1Q2</accession>
    <name>A0A1E5R1Q2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SWI5-dependent HO expression protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g4120</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1Q2">
        <property type="interactions" value="114"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1Q2"/>
    <dbReference type="InParanoid" id="A0A1E5R1Q2"/>
    <dbReference type="OrthoDB" id="5574718at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024660">
        <property type="entry name" value="UCS_central_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45994">
        <property type="entry name" value="FI21225P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45994:SF1">
        <property type="entry name" value="FI21225P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11701">
        <property type="entry name" value="UNC45-central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UNC-45/Cro1/She4 central" evidence="3">
        <location>
            <begin position="143"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="4">
        <location>
            <position position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="734" mass="83135" checksum="50299A0E641FC347" modified="2017-01-18" version="1">MSSVGDLCIKMASLSTSKQSFTNQEYISVINKSFEDSPIEFDWQACWLKSAKSNEVLKAHLLEQIASHPEVALEMYASSKQDNVPLIHLLVEAFGRKKIELHFLVEKYVKELNTRIIHFLISVLGSEKSRQTLKLSFFDLSFLIKIIPPLLSHDYTNASQNAMLCQLMMSVYDTRPASFEPVFQEYLEALCIEAEADINDAKSFTILTTTLKNFFPRIPNPCTAVFMSDEFQNLIRESVTKRSPTQVTIGKQIIQVLNASCNFPAMRQYVSENYASHLVQCLSISQLSVDAASVLVKMWDIKKLTELKTSLESLVDVFVFCITSDSTDSFEDVDVAIEALSYLTLKPSVLIKLREDGDLILSLLQIFNALDNSNFSTMYGIVLVLTNMCAPVGSDVDESDDDVEVRKLRDIAQANNKEPVQKPEDIYEFIQDYMINLGFLKSLLKRCEHFTTNIKXCLSQLLYHICCCNNKTKNKLFLEHCIPFSSFLLQCFQLDDKKYDGYSFKTLLKLGINLDPLKLFHNEEYALLMTIYLLKYLPKESEVEMKKNTLFKVLLCLGNLASAGNTFTKICPTIMLGSYFSRIESLMFSETPFIQTATLQMVLNCVSCSSGVLVKFFNFENPESQKNFRNLVKFFYLKDVEAQKAVTAIVAIGSEISFIAESLADEECLVEACSTLLDTQSADIELMERILFIIENCFSCASNSKIKQNETLRAALLNLENPLLKQGAKQLLQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1V3</accession>
    <name>A0A1E5R1V3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative vacuolar membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g4181</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1V3">
        <property type="interactions" value="432"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R1V3"/>
    <dbReference type="OrthoDB" id="1436450at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025016">
        <property type="entry name" value="DUF3955"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23051:SF0">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23051">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35, MEMBER F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13127">
        <property type="entry name" value="DUF3955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="356"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="395"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="430"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="462"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="487"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="520"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3955" evidence="8">
        <location>
            <begin position="91"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="397"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="170"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="170"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="231"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="558" mass="62449" checksum="2CD9BBF6C943C940" modified="2017-01-18" version="1">MSTKATYHSIEPKNDNIDKLHSVNNMSSSXKTTNNTNAGDIAAINNTNTSRIVTRNHTNATXERYSHDGYDVRSNKSMDSAHRLHQKKQWKLGLSLLAVVVIFWVLSSFLINTIFEDNSYRKPFFITYINTASFTAYLIIPLTKEYFRMKRTNNGKVDYRNLIMNALSTPPEXFTTPTQXTGNSANITSNNSNSNSNSNSNSNSNNNSNSNNNSNSNNNSNSNNTNKNNSLSMHPQVSKTKSNESTESDSLLANQQHQHTQESAYTGXYDLNPPTGSNNCTSTVEEVDILDEESYPLSLQNTIKLSAQFCLLWFLANFVTNASLSYTSVGSQTILSSTSSFFTLLISYLSRLDRSISYLKITGLIISFIGIVLITKSDIASTLVSKVSHPKLLTILFGNFLAILGALLYGVYSTLLKLKVKNVNRMNMHVFFGFVGLVTIVVLWPTIIILNYTGYEHFQIPYTRKIWFVILLNLVINFLSDYCWCKAMLLTSPLTVTVGLSTTIPFAMTGDFLFKGKKITTIYLLGALLILLSFFIIDREEKDVINHVEDDDEINIAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1V8</accession>
    <name>A0A1E5R1V8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative secreted glycosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3935</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R1V8"/>
    <dbReference type="OrthoDB" id="449263at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000224">
        <property type="term" value="F:peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006516">
        <property type="term" value="P:glycoprotein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.60:FF:000002">
        <property type="entry name" value="Glycosyl hydrolase family 92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.60">
        <property type="entry name" value="alpha-1,2-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1610.10">
        <property type="entry name" value="alpha-1,2-mannosidases domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2080.10">
        <property type="entry name" value="GH92 mannosidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041371">
        <property type="entry name" value="GH92_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012939">
        <property type="entry name" value="Glyco_hydro_92"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050883">
        <property type="entry name" value="PNGase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12143:SF38">
        <property type="entry name" value="ALPHA-1,2-MANNOSIDASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G10520)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12143">
        <property type="entry name" value="PEPTIDE N-GLYCANASE PNGASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07971">
        <property type="entry name" value="Glyco_hydro_92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17678">
        <property type="entry name" value="Glyco_hydro_92N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184595" evidence="1">
        <location>
            <begin position="20"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 92 N-terminal" evidence="3">
        <location>
            <begin position="99"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 92" evidence="2">
        <location>
            <begin position="364"/>
            <end position="857"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="880" mass="100732" checksum="4E965BA9CF8EC64A" modified="2017-01-18" version="1">MPRILILHYLLALFCGCLAENVNVNLKQSIFSEESIDEDKSFFLSMEEEAFMSLPNAQSSSSSYFQQDVDTASYKYYGPIKNESPYFKNLSAKYPYLKYIDVFYGTENGGHMFPGPNLPFGMCKIGVDILNPQEDSYSGYGPQGSFLGISMLHLSGTGGSPTYGVVSQVPYTDRDLSFIYEDHSFTGFXLENFANPKELQRAYPDYGHLGYYQVSLDNGVTIEFSNGYKSGIIKYEFPRVNICNTIFVNLTHHLHTFGRPWWTQNFESGYVKVNADHSSYEGAVVINGGWSSPDPYKVYFFGEFNQKFQHYVHLNGKLTYSKIFLNWGSSQNGSIGTYFQFANREMSEPLVSHIGVSFKSIFEAKRNIEIDYPDEHKFNLQYSVENAVQQWDLQVFSKFQSLNYSNENDMILEKFYTSIYGAHFMPTNKTGVDSPFYNEDCTDSLCEVVPYYDEWFTLWDTFRSLHPMLNIVNKPVGSELVRXLIEIWKHEDFMPDGRSAGRSGRTQGGSNSDIVLADAFVKNISHLIDWEAGFKAMRSNADIIPPYVRDPFAPDSTNKYGRGALKEWLELGYITRNFSRSVTRTMEYSYNDFALSVVAKGLGYNNLHEKYLHSSSNWQQIWNFDATLSDKSYKGFIGPKDANGEFNYKNYDPSSCFSCYWADDEYEGSPLEYGWAVPHDISSLLSFIGLNETFQLRLDDMFRLYGNGYANIGNEPSFLTPYLYNYINNNDRTSETLNYLTTVEFQTGVKGLPGNSDAGSMQSWLWFALIGIYPVAGTDVYLMSSPQITHWELREDPFYGAGRTVFEAENLYKNVTSGERNIYIKQVFLNGEILNRNWVQHHELFGPEGGHITFVMTDTAQHWDKDAMTPPSRGHFERNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R217</accession>
    <name>A0A1E5R217_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Vacuolar protein sorting-associated protein 29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g4063</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the VPS29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R217"/>
    <dbReference type="FunCoup" id="A0A1E5R217">
        <property type="interactions" value="1067"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R217"/>
    <dbReference type="InParanoid" id="A0A1E5R217"/>
    <dbReference type="OrthoDB" id="10258130at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11124">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN VPS29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="5">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="180"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="185"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="199"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="301"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ80941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="354" mass="38592" checksum="D3C03D30E6FFF112" modified="2017-01-18" version="1">MLILALSDAYIPERAIDLPIKFQKLLSASKNKISQVLLLGNCNKSVKFVEFLEQKVCDNIISIKGESDYQATQLPYNAIVPAGNFKIGLTSGFLIVPKNDVLSLLTISRQLDVDILLWGGTHNVEAYVLENKLFINPGSCTGAFNADWVMEEAEATDGESDLGEGTGSQVLEEEVSKLNISETGTDLDEAAADQEVEEADKAKNLEKASDVEDQKASSEASKAQESGKRGTGSKEPLEEDKADEPGNAKNAESEEKMHEDGHGAPVTYAETAKKNEDLSSTQHDKSVEKDTPQDNGSGSDNDQDESETDSDDDELVSSIPSFCLLDVQESVCSVYIYTYVDDEVKVDKVKYEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R244</accession>
    <name>A0A1E5R244_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">HECT-type E3 ubiquitin transferase TOM1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g4154</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the UPL family. TOM1/PTR1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R244">
        <property type="interactions" value="1124"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R244"/>
    <dbReference type="InParanoid" id="A0A1E5R244"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000004">
        <property type="entry name" value="E3 ubiquitin-protein ligase HUWE1, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010309">
        <property type="entry name" value="E3_Ub_ligase_DUF908"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010314">
        <property type="entry name" value="E3_Ub_ligase_DUF913"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025527">
        <property type="entry name" value="HUWE1/Rev1_UBM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF67">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HUWE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06012">
        <property type="entry name" value="DUF908"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06025">
        <property type="entry name" value="DUF913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14377">
        <property type="entry name" value="UBM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="11">
        <location>
            <begin position="3115"/>
            <end position="3452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="77"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="2048"/>
            <end position="2149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="2162"/>
            <end position="2184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="2547"/>
            <end position="2589"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="2398"/>
            <end position="2455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="99"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="116"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="132"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="2048"/>
            <end position="2062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="2070"/>
            <end position="2082"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="2090"/>
            <end position="2108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="2115"/>
            <end position="2142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="2162"/>
            <end position="2174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="2175"/>
            <end position="2184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="2550"/>
            <end position="2580"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="8">
        <location>
            <position position="3419"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="12">
        <location>
            <position position="2091"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00076267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ80978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="3452" mass="393035" checksum="9C4A9F3511E57239" modified="2017-01-18" version="1">MPVALDRIDQVIKEEKATLFKPLIDDLVESSEEDFLKKIDGIQEWDRSKDDLYVWIPVLNRMDSILKNIIDEHNLDITPSNALPSNTKPISDSDNAIKSESTVSDSHSSSLKTEESQITSQDTQQKLNATKESVKKEEDTKQEKPKAFLTLVDVKVENKIFTITSFTHRLLQNTSNRSIYSSLDRMYCLLNCPNMKVKLGAMKVLAINAERVGKLNTQMKPTAINGPAIKKALSIALSLLCSHTNDANQHYSLNDLMYRFCPKNNISHPEEMKKFPSKWKNLTFKYLSKKTSTVQYFKLNSSQLNQLSTQEIFNKITTEDDIPESQWFELSIQVFIAKIFSTSTQKSESKDFYESESIFDAIDILLQLKFYSIAYANCLLPSSQISSKYFEVDSYIFECLNDFILVAMPKKSDRISLEXIRFAALFALECISFKHVWCSDILRNLGGNMSHGAMFNMLKTIKKSLKDPQYQEYLNEDFNVRFFILISNLADVKQLNETLTANGLIQSLLDIISVSNKVAYRSLSSACNLLRVLISDFDTATQFFEAGGYSILVNRINAEVDFAINHPEFGNPSPYCKVYYSASFRQLSFLRGLLKQVLNLLSLDGSDRIRNLIDSSILKSLNLMLTNRPVFGFTLLAYALNVVQTMINSEPTVFPILVESDTIKIIVENFGSFVGPCSELLLVLPHVVSAVCLNNDGLDLVLKSDVVNTLLKPFTSLDMVKILTVDGSDLEYGAELDELYRHHPPVQKQLVSAFISTLSTIKDQVHFSKPLMNINKDGSTFYESPNEAVSQMDEAGNTIGYFDLESSGFIIRSFSAALSCCPWKPLAEQINPVDIWDMIFVKNLPFDFPSSDTFVYVCDVFSSLDYMDHQASLNDLLTRLEDTLDALKPVLDSQDSSKSFFISAEPDVLQKTASDLSTINSLLEALTCIYFDYATLSFPKVKLILNHFQREGSLIVIEKAVKAYRKVLLEEQAIRACLPPQVEADTREGFQHQKGPIVFYKSKLPSDLSLKPHLRTSAKFTNTYQLRYLCFFIQKSCASFFKSLLRMSNMRKNDMKGLDRRREVEIMECTMKSLLQNFDFEEPNIDANLSFLLSTLYTFGFVIANHSSSSALTKPRNLLSPITLSLFYRFDGHNKLLTLLEHLLQKIANNQYKAISTVVQHPVVYDKSNILVVAIINQALTLLKKTVQIEAIDESKNIKYYLGSDLGKCSFTGLFLVNAKYLIVERSKVVFSKELLSSLCDSKKLPDSVFNNLLDFFFCLFTKKLEEKNNDGTVLCYIQSDSVNPSKKKCDFLSNFDISEQKAQILLMENDNELPPAAALELTDQEQKRYDEALESEDLIKYYPEPMVRDEHICELSQKSRESFVGTLRQIGLVKLLSHYPTSAGFAAKLYDNMKNFSPGFFLGSQVLKSLLPELEENHGKMPLEELAPSIHTFSILLTHYFIPSTFKETIYDRFIDLFIEDFKPERANETWFARYLYGFEGLLSFNIIPQPEELKNPQKIRSDFETRIELSEQSKNKIFDCLIKIKNLNSTHSALSVARVLLLFSRDYDSAKIIAHSGILSVLLKSIGLNQKNEDVHMLESVFLLLVRACFETKGVVKALIENELKKYFGLSVLRKPRVLPALLMEKTNLVLRDSASFIDVICENMRFADFDESNELAAYVLNVKENAPWDKDQQSSDQSKMTLDNKTGIVQLLFSQLIAAFKKDWLSESQEDNDLLSSQTSKNSRKQSSQKNPVYAYISFLLRTLTEIVASYKQSKLELLCFSRRNTFGSPHVKPRTTFINFLLYQLIPLSQDIEDKAEAKKREQLSRLAKELILAFMVSVSRSKPATKEDKYVDPDMTFIRKFTIECIMKALTNSAKNITTHTESPKNIRSWFDLLGHILTIQFTAMFNVSETNSLNNDKFHFCKLMLEMNVPSSITSIMASMDLNYPQSKPLMNHSIEPLKLLSYIRDKHPEQFSVENQEEDDDVDVESDEENYDMFKNSALGMYEVDDIDEDEFDEESLLGDGDDDEDIAFVDADGMEEEGIEAVFTDEEDEGIRSRHLEDVEDEGIDLESEGEVIYEDNNIMHDDEEEEEDDDDEEEHVHSDEELGIMDGEESDFENEDDADMISISDLDNEEQAILYEDEDDEMSHEETDLEDASGNDSPALGEINVELYDSQLEQSDWDSGTSDLLSENDSEDMEESFINDEEEEDIGDDAGENVFSDLLQVTGRAGSEDNEVHIIRFDELNNMASRIFGSGRRDSHSLADNNRLSVHGLLQRGSRRHSHGVLNHRGSFSRGMFENMNLPNERDVMSEFFLSPVHPESDHPLSGFNSVRSDFDLVSPPPNRANGSGRFSPFSDNGNLPILSAFFRDPQAGPTLKNTTSRWSEIVDMFYIRKSFSLNIIPAIINRIYEDSISLHKKNISEAEKIEKEKEKEKEKERMQNMKRDYSSLIDEEEEEAIISEEEEQEEEGAPRAHSYVQIGGQEVDVASTDIDPEYLNALPEELREEVFYQHVAEDRARRLSHRDSESHIRPGYLSTLPENIRSNILEQQVRIQSDPLFVEEPTAHESSSAISPLSTETTNQSSNDMNESQNNTQPKSKKSKHHFPPLVDRAGIFSILKSLFLPQPYLVREKYHEFFRHLSNSKQNRYDFLNLILFILIEGIVSKSSLDRVYNYICNKANQLKTSSKKSFEAHHFQLPLDCTPTSVANQCIDALQVLVDHNDSVKLFFITEHDNLIVHKPISSTFHSGSQSSSSNSYTSEGIVSNENKKWPINYLFNLLSNRIVTDEVVLMDLLTKILQDCTKMMKNMAKSKKNKLNVPVIDEKLYPLTVEVLNLDSCNTKVFQQTLTVMLNLSIINKEESKFNXLLIENARSSLAFVIPELKEITSNVNKLNEPDSQTSELITKFSSPISSQAKLLKVLTSIDFIATGGLKKLSPEATQQVAHKVEKIYLKINLGELWSCLSDCLSKFETKINLDNEKQRAQVSSVSYLLPLIESLMVVCKYSDTSEFRDSLSETDKHISNQCKDDLNALPLNKLFFHFTDSHKKLLNQMIRGNPKLMSGPFSVLIKNSKVLDFDNKRYYFMDKLSRDGGSAGNDVSNDHHNLHSAASKLPIEVTRDQVFLDSYRALFFKPIKIFKESKLDITFKGEEGVDAGGVKREWYQSISKQMVNPDYALFLSASSDSNTFHPNRTSWVNPEHLSFFKFIGMIIGKSIRDRCFVDCYFSRAVYKQLLGIPVSLKDMESIDPDYYKSLLWILENDITDIIDLTFSVDTDDYGEHKIIDLIENGRDIAVTQENKSDYVTKVVEYKLSTSVSEQMSNLLQGFYSIIPKKLITIFNAKELELLVSGLPDIDVLDWKNNTQYINYTASSREIGYFWRAVRSFSKEEKAKLLQFVTGTSKVPLNGFKDLAGVNGVSKFSIHKDYGATDRLPQSHTCFNQLDLPAYESYEMLRRALLLAISEGHEGFGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R254</accession>
    <name>A0A1E5R254_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cys-Gly metallodipeptidase DUG1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Deficient in utilization of glutathione protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">GSH degradosomal complex subunit DUG1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g4360</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="13">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R254">
        <property type="interactions" value="368"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R254"/>
    <dbReference type="InParanoid" id="A0A1E5R254"/>
    <dbReference type="OrthoDB" id="7832001at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070573">
        <property type="term" value="F:metallodipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05676">
        <property type="entry name" value="M20_dipept_like_CNDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.360:FF:000008">
        <property type="entry name" value="Cytosolic non-specific dipeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017153">
        <property type="entry name" value="CNDP/DUG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051458">
        <property type="entry name" value="Cyt/Met_Dipeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270">
        <property type="entry name" value="BETA-ALA-HIS DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270:SF4">
        <property type="entry name" value="CARNOSINE DIPEPTIDASE 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037242">
        <property type="entry name" value="CNDP_dipeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0224">Dipeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="15">
        <location>
            <begin position="265"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="387"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="474"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="502"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="502"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="14">
        <location>
            <position position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00080668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR037242-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR037242-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR037242-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR037242-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ80989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="531" mass="59158" checksum="6314A08DCC3A0D86" modified="2017-01-18" version="1">MLERLTKTNXLQFTTQLHFLPKSFVRNISMNVRLPKNFPNHNQNQQTRTMSSVEERLQPVFAKIDELKPSFISKLAEAIAIPAVSGDEELRPKVFEKMEWVAGQLEKLGCHDVKLRELGLQPPPVATPNLKLPPVVLARYGSDPAKKTVLVYAHADVQPASIEDGWNTEPFTLKVDEEKGLLIGRGSTDDTGPMLCWFNSLEAHQKAGVEIPVNLVFCIEGMEESGSLGLEQMIKEEASQYFKGVNYVSISDNYWLGTTTPALTYGLRGVNYYQIVVEGPKADLHSGVFGGCISEPMIDLVQVMSTLVNSRGEIQIEGIKEMVAPVTKEESAIYEKIHYSLEEFNESSNSETCLFDNKEDILMHRWRYPSLSLHGIEGAFSGSGAKTVIPAKVVGKFSIRTVPNIESKKLDEIVINHCNKEFAKLNSPNKFKTELFHDGNYWVSDPYNDNFTAAAKATKLVWGMEPDFTREGGSIPITLTFEEELKCSVVLIAAGRGNDGAHSINEKMDISNYMNGAKLLGSYLHYLAESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R286</accession>
    <name>A0A1E5R286_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein transport protein SEC23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3947</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">COPII-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC23/SEC24 family. SEC23 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R286"/>
    <dbReference type="InParanoid" id="A0A1E5R286"/>
    <dbReference type="OrthoDB" id="3971671at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037364">
        <property type="entry name" value="Sec23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141:SF0">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="1" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="1" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="unsure residue" description="E or Q" evidence="2">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="705" mass="81216" checksum="3D90942840628EF5" modified="2017-01-18" version="1">MPIYSSHNVLPKSKRDGDKYFPELLEPNNPGGYAHFLFEPDLKQENSISFVQDTKCLSCGASISPYCLKTDASWKCIFCQEPNYFNQKTNTVDVNNVPREYTAQMHLSENISAHTINGYRLLLVFDACVKDEFELKQYKNYLIALTSKLSTCSVDYLEIACFWIFPDSEYYQLTHEGTIAKCKNKDFFLKKQGWVDLQQFQHLVFSFVEFVKIQKVKPGKRLKRNIPFEIFENVPLQNESARYIYKANILLFGGCTTKDGKIVNNELKNTIRDFKNHSVSAFRKASNFYSSTLSRNAAGTKLPTPMTYHFFICSLHDCGIWEMFTGAKDRCDLVMFLDEFEGSFFQHLXQKWWENFFITYNKLKXFSSSGKVFKNCGVSNVGLERVANKFTDSTMMSDVAIGFKGTQFKLNNSSVQNYCATFHLEGIKNLESKDHQHRLAQTIVSATQFPTAVIVQFEIEYYVGLAQYKKVSCIEIPVVDNCEEHLIVNTDVLSVVLGKQSLHHLWRFAGTNLSNIEWKIKETHQSINSLLQIRFSSISNKKHVILDHEQLLQRIYMMKFSTKLLDITNVSPDEYIKESLIKWGGKTQYADLVKELKPKLWTQASPLDMKNLQEIAFDLNHPLVTSVTIHSNMLLEVADSYILRGSAEFLSAFSDQCSRFDKPTIDVVEGQSKDRLVVHKVYPSIFLAKQNVLFDYSLYKQRLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2A4</accession>
    <name>A0A1E5R2A4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Pyrimidine pathway regulatory protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g4001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2A4">
        <property type="interactions" value="187"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2A4"/>
    <dbReference type="InParanoid" id="A0A1E5R2A4"/>
    <dbReference type="OrthoDB" id="2399539at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052202">
        <property type="entry name" value="Yeast_MetPath_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782:SF1">
        <property type="entry name" value="PYRIMIDINE PATHWAY REGULATORY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="618"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="663"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="10">
        <location>
            <begin position="99"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="264"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="377"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="459"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1063"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1145"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="264"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="291"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="348"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="464"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="506"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="520"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1147"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1168"/>
            <end position="1219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ81037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1271" mass="142980" checksum="CE96F624567102A0" modified="2017-01-18" version="1">MDQDNLDSPTKNRNNATDLESSTNRSTEEGSSVXNETHREKATSKKVLKKNAHGSKKSEKPQPIKKEEENKPDHNLASKGQNHNRPSSSVIGISRVIASCQRCRLKKVRCDNQFPSCTRCAKMNLSCVSIDPATGREIPRSYVIYLEDKLYACMLKLKELGVDLSEADLKTNIPCTSXDPPCNIDLYQERFRDQHTLPPENVMAGYVINKGTSMQKGVPSSLVLNELSESSMTSEKVYSMKMQDSQPLKEADDALSVEKEHTKVLNTPLLQQNDTTPLSSSGLDPHHSSSADIPQTNXTLQEQXSPTTTXHGCLASSSAASQSNLLKLQRESKMLSKKPRLGARPASHASTPSNSYLGDSSGIPFAKLMFTAVNFEPSSDNSLNESEKESEQNQTSDYSDADIKPPSVSDNINHRFLPTKKEALNLIQTYFVDSNTQYPVLHRDFFIKKYFEPIYGEFPKRHQNNTASGSKSRNVNDTSGASTPINNTNTSAKSASPPSMFQPNERLSEKGQGTRDETASTHSNSSTGEKSGRKTFVHSLANDFTNMNDNFKLLPEHSLEKDEQYVKLINKLSSTGSFSIDGAHDPNNEKKTVIPLCDIISLIPADEEIPLVYQVPLFFINMVMAIGAGSRVLISGTVKSIIFKKRATRYMNSIYASSNRLEALSGLLLLSIYSLMLPNVPGVWYVMGNSLRLCVDLGLHAEKLNKNYDPFIKDMRRRLFWVTYSIDRQICLYFGRPFGIPEESITTASISELDDALITTNNDGIEDYSHLKTSGSSYKSISLAFFKIRRMQSTLLQVLYAPRGVVPKPFDNVEIWRKGFDRELLNWYNKSVPKTTKKMNCSFKKELFKLNLFHTRTMLYGISPKFSIPNDQACSILTETTKGMMDQYYKIFLNKGFNYTWVAVHNLFMCGMTFLYTKYRNTDLDEFDEFKDHMLAVLKSLFGTCEAAPNCFKIFRILSKAVKKLILQEISKKASNLEATPLIRHQSVPLSTANVGNMTGLHTGAFARPDPFVAAQPPATAHRSEGSAGIESITTSVNQEFNSIATDDEMRKFFLELENASPYSNADSAGLPDRSPTTSSRSGVTLDDDSAFRPSSFQHAPLQNQPFQMYPQQLSFMQHKIQQPMMDERFSNRTXYAPINHNYASWQQQQQQQQQQQQQQQQQQSQQENMVFTEASSHPLRHTQSYPFPESMPSSNXNNAFPHSHVQSSQHHNDPNGSLQKQVLTQNMNTQNKISKDEQRIYEMICNMGTDSIWDKYLGASDEYFDSFNTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2C1</accession>
    <name>A0A1E5R2C1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ethionine resistance-conferring protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3913</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2C1"/>
    <dbReference type="FunCoup" id="A0A1E5R2C1">
        <property type="interactions" value="136"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2C1"/>
    <dbReference type="InParanoid" id="A0A1E5R2C1"/>
    <dbReference type="OrthoDB" id="2126698at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="276"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="322"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="352"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="384"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="583"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="612"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="39"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="60"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ81055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="651" mass="72046" checksum="65EF0740C23C3DB3" modified="2017-01-18" version="1">MSKQFKHSDMRRKSSVLYSTSVGKGGLFNPQDLLYSSSQGKNVFLENENENENENEAGSREQSPLASLSSGNASYNTAKTQHHQQQGEQAHLLNNEPQAGYSSMDNLLTAQQQKGKPHGYSPLREEIGQDLENGESVQQSNQSTVNNSASEIYPELLEEERELLLDNKLIDSTDHDSSDIAAKWASANPEMLSTSYHREFKTLVKNAAPLVLTFLLQNSLSLASIFSVSHLGTKELGAITLGSMTANICGFAAIQGLCTCLDTLCSQAYGAKKYHLVGVFLQRCCALTILGFIPVQILWYFFSETLLKVFIEQEELCYLASKYLKIMVFSLPPFIVFECTKRFLQCQGIFHASTLVLLVCAPLNAFMNYLLVWNPYVGVGYLGAPISVVLNYWLMCLGLISYIVFTKNKINPRKCWGGFVQYDQIFNNWKQMINLAVPGIIMVEAEFVGFEILTIFAAKLGEDALAAQSIVATIASFAYQIPFSISISTSTRVANFIGAGLWKNCLITCKSALSLSFVISCTNLSIIFLLKHKIAQIFSSEPVVIKLVEQSLPILAVMQLFDAFNACTAGCLRGQGQQRKGSVINILAFYIIGVPMAYFLTFKLNLSGVTGLWLGIVNALIFMTVCQSMAVFNVNWKDLVKEAEARNEDPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2J9</accession>
    <name>A0A1E5R2J9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Autophagy-related protein 31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4218</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R2J9"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.270.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018621">
        <property type="entry name" value="Atg31"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09795">
        <property type="entry name" value="ATG31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="25295" checksum="B4FB275079FCCD9C" modified="2017-01-18" version="1">MPKLSDPQVSSQDSLEFEDHINDEVVPLLISVVDENVNKLDDNQWYLTKNIHYIFTDDEEDQEDFEDQDYKDEEKSVEIERXXSKNEPDXSVESETPKLFSKPALTSDPSSREXVIVVDVDKHFRLQNAELISDNYRLLDCSAIQKTRPFDVSRNXSATTSREKSNTLQISLESTDIKLTVLSKFCHDPHIAETHSLSELITIYKKQNEQLFNLLDTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2K3</accession>
    <name>A0A1E5R2K3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Peptide hydrolase</fullName>
            <ecNumber evidence="15">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">AWRI3579_g4254</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in vacuolar sorting and osmoregulation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Vacuole membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the peptidase M28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="15"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2K3">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2K3"/>
    <dbReference type="InParanoid" id="A0A1E5R2K3"/>
    <dbReference type="OrthoDB" id="76293at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03875">
        <property type="entry name" value="M28_Fxna_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048024">
        <property type="entry name" value="Fxna-like_M28_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045175">
        <property type="entry name" value="M28_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053975">
        <property type="entry name" value="PFF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053976">
        <property type="entry name" value="PFF1_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147">
        <property type="entry name" value="METALLOPEPTIDASE M28 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147:SF58">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22250">
        <property type="entry name" value="PFF1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22251">
        <property type="entry name" value="PFF1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6 15 21" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <keyword evidence="10 15" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="372"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="405"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="437"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="468"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="499"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="609"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="652"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="673"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="18">
        <location>
            <begin position="134"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar membrane protease transmembrane" evidence="20">
        <location>
            <begin position="407"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar membrane protease C-terminal" evidence="19">
        <location>
            <begin position="702"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="562"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="21">
        <location>
            <position position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF22250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF22251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OEJ81136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="960" mass="109632" checksum="FA5CB374D44C0D7A" modified="2017-01-18" version="1">MGVLRNLFRYRRSSVSILTLFFYVSVALYFYIYNEGVPQMNYSNEDAYLLDPAWLHLQEITKHPHPYSSHENDRVHAYLFQQINKAIMESGRPEHVSVSDDEYDKDGLKALFKQHDVFDAHSTATRLIYYESSNILVKIDGTLGDSVPGLLLSAHYDSVPLGNGATDDGKGIATLFALFQHYMDNQPARTVIFNFNNNEEFGLLGASIFGYHPWHKLVDSVINLEGTGTGGSKAVLFRTSDTQMAKIYKESVKDQPFGNSMYQQGFYNRFVGSETDYKVYQEQQGLRGWDIAFIKPRDLYHTWKDSVRYTDRDSLYHMLHTAFQLSNYALNENLXNYKDDQYDAXNEKDDLSPAVFFDLGGKFYAFSARSLFQINIVALVCFPILIIVTLLLVNKSNLESVWTWAKLPISMAVSFLIVSLWSNFLQDHNVMIVSRNYWVPIMSYASIFLLSHYFVLRFILKAGYKYKTVAMLEVNFAMWVYLTNVTIQLKKHDYKDTGAYPFSIFYLLLSIGTVCGILLDPHHPVAGEEETFFEHSSDELNNLNDEPTRLLNGSNITNHKNHSPSYGSLDHEAGGTGTAASSEDGPLHDIEQRVDTHEKKNAXPAYYGWLLQYLVVVPGSLFILFNAGGLIIDALNQTVQDGDVFTDKIYKFIPLIAILIIVPILPFVTRLNYITILIVNCIMVISVIFSLTSEPFTESLPLKIRCGEYWDLNVEPHLGQIYTFGREVTSNFMESVIMDLPSQKLSKHHTPSCTYTNGNALCKYKTIPLTDGHRFNSSTTPAKNTPYNISXEVLTNNRKDPDRSKYEPISSRFRINVDDNRACTLRFPQAGNNLREVYVFDGENQKDRSEAFYFVKPDKGIDELQLHKLNFSKPYHEVELVWLPQILVNEGGNGNGNTQDELKVDVSCYWGDYITFDEQDNSKVKVNSPGLMELFNYSPKNVIYTNREKGMIISEKSYIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2L1</accession>
    <name>A0A1E5R2L1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">tRNA-specific adenosine deaminase subunit TAD2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4028</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2L1"/>
    <dbReference type="FunCoup" id="A0A1E5R2L1">
        <property type="interactions" value="514"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2L1"/>
    <dbReference type="InParanoid" id="A0A1E5R2L1"/>
    <dbReference type="OrthoDB" id="3970845at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052717">
        <property type="term" value="F:tRNA-specific adenosine-34 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002100">
        <property type="term" value="P:tRNA wobble adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01285">
        <property type="entry name" value="nucleoside_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079">
        <property type="entry name" value="CYTOSINE DEAMINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079:SF149">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="4">
        <location>
            <begin position="4"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ81147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="249" mass="28513" checksum="F4DAFB58CACCE398" modified="2017-01-18" version="1">MKIGYELKHMETALKLAKYALDHGETPVACIFVHEPTNSVVAFGMNDTNKSRSGTSHAEFMGIAQILELQKKDEFPEHAIRFEDITLYVTVEPCIMCASAVQQIGIKKVIFGCGNDRFGGNGSVLSIQQDSYVVLPGILRKEAVLMLRNFYVMENDHAPEPRTKTNRKLDLQEFPPIPWKNYISEIDFKDTFGETAYREKYNSLEDLISDEKDIKWDLLEGRYDDILEILDSQITQKFTYKKQCIVKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2L9</accession>
    <name>A0A1E5R2L9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C-1-tetrahydrofolate synthase, cytoplasmic</fullName>
            <ecNumber evidence="8">1.5.1.5</ecNumber>
            <ecNumber evidence="7">3.5.4.9</ecNumber>
            <ecNumber evidence="6">6.3.4.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">AWRI3579_g4026</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>(6R)-5,10-methenyltetrahydrofolate + H2O = (6R)-10-formyltetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="3.5.4.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:23701"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="22">
            <dbReference type="Rhea" id="RHEA:23702"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-methenyltetrahydrofolate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22812"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:22813"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="20">
            <dbReference type="Rhea" id="RHEA:22814"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>(6S)-5,6,7,8-tetrahydrofolate + formate + ATP = (6R)-10-formyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20221"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:20222"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="21">
            <dbReference type="Rhea" id="RHEA:20223"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.5" evidence="8"/>
    <dbReference type="EC" id="3.5.4.9" evidence="7"/>
    <dbReference type="EC" id="6.3.4.3" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2L9"/>
    <dbReference type="STRING" id="56408.A0A1E5R2L9"/>
    <dbReference type="InParanoid" id="A0A1E5R2L9"/>
    <dbReference type="OrthoDB" id="5126881at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004329">
        <property type="term" value="F:formate-tetrahydrofolate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009257">
        <property type="term" value="P:10-formyltetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046655">
        <property type="term" value="P:folic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006555">
        <property type="term" value="P:methionine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00477">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000006">
        <property type="entry name" value="Bifunctional protein FolD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000245">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001123">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000005">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.770:FF:000001">
        <property type="entry name" value="Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.410.10:FF:000001">
        <property type="entry name" value="Putative formate--tetrahydrofolate ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.410.10">
        <property type="entry name" value="Formyltetrahydrofolate synthetase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01543">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000559">
        <property type="entry name" value="Formate_THF_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020628">
        <property type="entry name" value="Formate_THF_ligase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020867">
        <property type="entry name" value="THF_DH/CycHdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF5">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01268">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00721">
        <property type="entry name" value="FTHFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00722">
        <property type="entry name" value="FTHFS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00766">
        <property type="entry name" value="THF_DHG_CYH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00767">
        <property type="entry name" value="THF_DHG_CYH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0436">Ligase</keyword>
    <keyword evidence="19" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="17" id="KW-0521">NADP</keyword>
    <keyword evidence="13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic" evidence="23">
        <location>
            <begin position="5"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding" evidence="24">
        <location>
            <begin position="144"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00017592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00051435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00051544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00052163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF02882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="OEJ81141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="947" mass="102533" checksum="9B6C45BE29FDFC01" modified="2017-01-18" version="1">MSQIIDGKAIAKSIRDDIHTKINELQAKNPNFVPTLAIMQVGNRPDSATYVKMKLRAAKESKINVDFVHYADEANVSEHEILSKIDDLNNNPNVHGIIVQLPLPAHLNEDLITSKVSPKKDVDGFGSYNIGELNKRNGNPFFFPCTPKGIIELLKKSHVELEGKNAVVLGRSDIVGAPVAQLLNLNNATVTVLHSRSKNIEKHVANCDILIVAIGKPEFVKGEWFASNKNSPVVIDVGTNYVEDSTKKSGYKCVGDVEFEKSLPYVKLITPVPGGVGPMTVAMLLENTFIAAERSISSEPTAKFQALPLKLVKPVPSDFEISRAQKPKEITQVAAEAGILPYEVEPYGSTKAKVKLELLERLKDREQGKYILVAGITPTPLGEGKSTTTVGLAQAIGAHLNKKVFANVRQPSMGPTFGIKGGAAGGGYSQVIPMDEFNLHVTGDIHAISMANNLLAAAIDTRMFHENSQKDGPLYKRLVPTKKGVRKFTPTMLKRLEKLGINKTNPDDLTKEEAGRFARLDIDPETITWRRVVDCNDRMLRGITIGEAPTEKGFTRNTGFDISVASECMAIMALSNDLKDMRERLGKIVIASSRSGEPVTCEDIGCAGAMTALLKDAIKPNIMQTLEGTPVFVHAGPFANISIGANSVLADKMALKLAGVEPGLSLAEKQAKQGYVITEAGFDFTMGGERFINIKCRSSGLVPDVVVIVATVRALKVHGGGPDVKAGAPLPAEYLNENIDLLRKGCANLVKHIQNVKQYNLPVVIAINKMSSDTDLEHEVIKEEALKAGAQDAIVSNHWEEGGKGAVDLAEGVVKACESDTSNFQFLYETEGPSVEEKITSIAKNMYGAGEVEFLPEAQKKIDLYTKQGFGNLPICIAKTQYSLSHDANLKGVPTGFKFPVRDVRASIGAGYLYALAAEIQTIPGLPTHCGFHNIEVTEDGEIEGMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2P6</accession>
    <name>A0A1E5R2P6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="14">1.2.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g3868</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = (2R)-3-phospho-glyceroyl phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10300"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 14">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.12" evidence="14"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2P6"/>
    <dbReference type="FunCoup" id="A0A1E5R2P6">
        <property type="interactions" value="1293"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2P6"/>
    <dbReference type="InParanoid" id="A0A1E5R2P6"/>
    <dbReference type="OrthoDB" id="1152826at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00184"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030312">
        <property type="term" value="C:external encapsulating structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18126">
        <property type="entry name" value="GAPDH_I_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05214">
        <property type="entry name" value="GAPDH_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000001">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000020">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006424">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01534">
        <property type="entry name" value="GAPDH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836">
        <property type="entry name" value="GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836:SF76">
        <property type="entry name" value="GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 14" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 11 14" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding" evidence="15">
        <location>
            <begin position="2"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="11"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="148"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="208"/>
            <end position="209"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Activates thiol group during catalysis" evidence="12">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ80843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="331" mass="35507" checksum="36410E563957F562" modified="2017-01-18" version="1">MVNVAINGFGRIGRIVLRIALQRKDINVVAVNDPFITTDYAAYMFKYDSTHGRYEGSVEHTADSLVIDGKTIKVYNERDPANLPWGADKVEIAIDSTGIFKELDSAQKHIDAGAKKVVITAPSGTAPMFVVGVNETEYAGQNIVSNASCTTNCLAPLAKVINDAFGIEEGLMTTVHSMTATQKTVDGPSHKDWRGGRTASGNIIPSSTGAAKAVGKVLPVLQGKLTGMAFRVPTVDVSVVDLTVKLAKETTYDEIKKVVKEASEGKMKGVLGYTEEDVVSSDFLGSSYSSVFDAAAGIQLSPKFVKLVSWYDNEFGYSTRVVDLVQHVASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2Z2</accession>
    <name>A0A1E5R2Z2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="9">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4384</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="9"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2Z2">
        <property type="interactions" value="424"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2Z2"/>
    <dbReference type="InParanoid" id="A0A1E5R2Z2"/>
    <dbReference type="OrthoDB" id="5575at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001076">
        <property type="entry name" value="ATP-dependent DNA helicase MER3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000012">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052247">
        <property type="entry name" value="Meiotic_Crossover_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47835:SF3">
        <property type="entry name" value="HELICASE FOR MEIOSIS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47835">
        <property type="entry name" value="HFM1, ATP DEPENDENT DNA HELICASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0469">Meiosis</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="128"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="343"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="13">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="13">
        <location>
            <position position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ80943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1179" mass="133198" checksum="B5B5C61A149AD9DA" modified="2017-01-18" version="1">MNTKNSYLQKLMNFNVGIKHNSLLDNSDESDVEPHXNLKRVSKSALFXDMVFDDTASSVESSFQHGXAXNKRKVLVKKRKRQMVHXLAPAXASQQSLRLXSDXPDSFNGFFTFTKFNKMQSEAFDYLYGEKVTKXNCIISAPTGAGKTVLFELAMLNVFRQTTDYKNIKFVYLAPTKSLCMEKFESWKAKFKKVEVGFLTSDTSEPDAEVLVKSCILVTTPEKWDLLTRKWTEYKKTFSMLRLCMVDEIHTLKDTRGPVLEXVLTRMNRLCPNMKIIAVSATIPNIXDIATWLRDYQTNEHALFLEYDESYRAVQLEKHVIGYPMSSNNDFQAESTYTSRIPSIVEKYSNAKPVLIFCSTRNSASITAKYMAKNYSRAKNKINGLADEVSESQLKECCKHGVAYHHAGLSYSDRKLVEKMFLTGXXEILCSTSTLAVGVNLPAYLVIIKGTKMWNFSGYKEYSELDVLQMIGRAGRPQFETSGCAVIMTSHENQERYDKLLSGTENLESSLHLNLAEHICAEIELGTIESRESAXEWMEHTFLYTRFKRNPQFYKLKGSNSASPDSVLRVLCEEVIEKLIRCKVVVASKNSTLECTEYGVALSKHYXSLNTLQVFVECSPNCSLSAVLKLLSKADEFSGLKVKHNEKTFYRELNQMQGIRFQHKDKHKVNPVIHETEQKISLIIQQELGGVDLSGCRDYGRLSHALNQDKFFLFKHCSRILKCMVDCFVKKRDGTSLKNTLLLMRSINGKCWDDSPMVLRQIQNIGVASVGKLSKHGVNSIKHMKMLTATQIEYYLGLNVGGGIKYKNSAALIPKFEINVSEPIVKNLGSALLGTFKIKIKARFPNKSPARQKHYADILCVRSDGLLVDFRRILVSKIENSKVFTLEAEICDSEKTFCFSIGCEEIAGINESCVIDIKKFVPADVXRSVGLFLEDSDTDEDIIQLLHHKXYREQASIENHNNDHNGNLAKESKRKRLGNGNFACLHTCKDKMSCRHLCCRDGVLECVPKFNSRNHTETTPLAVPKYERQNILPSANEXKETCIKMPKLRNACDVDVLSLSSTSEDDEPVNAANNYSVTVYSPPCAQTQISNSMRFGVPDEDKDELLKEVKELTRRTNLXKSSETKPVIATSLXKECXQNEQSAHYDEILNKSQNSIADSVMSKDASCSLGFLGSDVEFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4U0</accession>
    <name>A0A1E5R4U0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Peroxiredoxin-5, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3767</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peroxiredoxin family. Prx5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4U0"/>
    <dbReference type="FunCoup" id="A0A1E5R4U0">
        <property type="interactions" value="566"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4U0"/>
    <dbReference type="InParanoid" id="A0A1E5R4U0"/>
    <dbReference type="OrthoDB" id="1882547at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03013">
        <property type="entry name" value="PRX5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037944">
        <property type="entry name" value="PRX5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430">
        <property type="entry name" value="PEROXIREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430:SF39">
        <property type="entry name" value="PEROXISOMAL MEMBRANE ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 7" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="5 7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Redoxin" evidence="8">
        <location>
            <begin position="35"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="6">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR637944-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ81929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="189" mass="20436" checksum="4B3A6134B1261592" modified="2017-01-18" version="1">MLSLRKNLVQIGLKSKFHTVSPQLFKAGDKITGQFPGLVEDSPGNAVDLAKDLSQYKKAIVVQVPGAFSPACSASHIPGYLSQLQKFHDKGVQEIVFTTVNDPFVTKAWKKDLKFPEGAKAGVKLLADSEGSFAKSGDSLFDDSVKFFGNKRGTRTALVVENGEIKQVFEEPDKAGVNVSSADNVLKHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R525</accession>
    <name>A0A1E5R525_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Autophagy-related protein 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3503</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the ATG13 family. Fungi subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R525">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R525"/>
    <dbReference type="InParanoid" id="A0A1E5R525"/>
    <dbReference type="OrthoDB" id="3972378at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.10">
        <property type="entry name" value="HORMA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040182">
        <property type="entry name" value="ATG13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018731">
        <property type="entry name" value="Atg13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036570">
        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13430:SF4">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10033">
        <property type="entry name" value="ATG13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0072">Autophagy</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Autophagy-related protein 13 N-terminal" evidence="6">
        <location>
            <begin position="14"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="324"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="454"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="634"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="265"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="324"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="351"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="469"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="480"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="634"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="644"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ82015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="765" mass="84168" checksum="72E94BE4876DDEAF" modified="2017-01-18" version="1">MMDSHSLDIVAAMIKVFFQKTATLISNSKTEAFGVHADKDDVLATLNEFYDLNAEKNIEPWLNINSQNMSKVAPLVIETYLDLRNLDSQHTVYLKDMKDNRNWNVCKGGKKLEIVLERWLLELDTETVSQQDQQANLDEDDLSYFLQNKLDLLLRYLHTLITLLPATDLYSKLLKNASNESSLDSLLKVSTRILDGTKPILSRGRVGLSKSIIPLENEPGATHLEQKRVPPIATPIGLLRVNVSYRKNTDFEITEEVNKLSSFSNTTTTTTTKSGKSQNTSPATNTIVVPRVKTPSSNIHFGSFPSSSPHTYAGSLMSANFSSSNDHNMSSSLASRKSSTHARPSLQPFKVGSVGSGSSAVPLARNPSNSSVIATLRAQRSSNGSINAGTPVGTILNNTTNSTPPVVPGNTGIGVSPINTISNNTLYNHPNDGLSRNSIEARFSSSFDKVRRHSSVRKSDESIRAFRSRTGSLDPQQQANNVSSSTTNTREXTTLATDKLNEFXVAEALHSGNEDEILDFMRMLDEKKDLKLTGRSNVLASTPDVSESHFSHTASSLKNEAVTDHKPKENVTDSLIRFKMMKLGNDNFTENLSMSISINNPDNKSHDNFDDAEPPFGFSPQHGYDLSALRASFDKRRQSEGSSRKSGNSTSNDELLGRGYSKSPHDRRPSNSSLPPGSHHLLNSDLLKSSSPTYSLNRYANSLSKTNSVGVQTEKFFEPTTASAAAYGNFHKPKEMSFASKNKEKLASNEDDDLLFFMSDMNKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R550</accession>
    <name>A0A1E5R550_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3731</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R550"/>
    <dbReference type="FunCoup" id="A0A1E5R550">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R550"/>
    <dbReference type="OrthoDB" id="1872155at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071821">
        <property type="term" value="C:FANCM-MHF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22919">
        <property type="entry name" value="HFD_CENP-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029003">
        <property type="entry name" value="CENP-S/Mhf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15630">
        <property type="entry name" value="CENP-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ81998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="117" mass="13721" checksum="461B6C887E7EEFE9" modified="2017-01-18" version="1">MLVTGTTNEYSQLDNIERSKSSCRSNNFEMDEEQLLWCKISKQVDAISNEENIAINDTFKNSLNELTKEKLKQIGNDLQLFMRHRIQDPSKQSKTITESDFKLFLRNNPELYKELSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R581</accession>
    <name>A0A1E5R581_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ubiquitin-like protein MDY2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3776</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R581"/>
    <dbReference type="FunCoup" id="A0A1E5R581">
        <property type="interactions" value="73"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R581"/>
    <dbReference type="InParanoid" id="A0A1E5R581"/>
    <dbReference type="OrthoDB" id="4067208at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.286.70">
        <property type="entry name" value="Get5 dimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040474">
        <property type="entry name" value="MDY2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031765">
        <property type="entry name" value="Mdy2_get4-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16843">
        <property type="entry name" value="Get5_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18514">
        <property type="entry name" value="MDY2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="73"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="207" mass="23134" checksum="9ACA4BAFF8FD8BE6" modified="2017-01-18" version="1">MATQLTEKEFVSNFLTLATIHPFKYSKDHFKPYEDVEQLGVALPALPCKKYKYQPRSATAAFLESASRSTDNVELSLKSVRPPKFSISKEFRKNQSIHAVKEFLVNEIEELVETQQLKVLLKGKVLHDSYILSDLSTDSAALTVMVSKPSTPVATTSAPVSSPATPTPTSKVVEMPWTEIEELLKIRFGQDAATYVARLQKGWELTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R598</accession>
    <name>A0A1E5R598_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pre-mRNA-splicing factor SPP381</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3728</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R598"/>
    <dbReference type="FunCoup" id="A0A1E5R598">
        <property type="interactions" value="143"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R598"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="19155" checksum="57390B795606C6EA" modified="2017-01-18" version="1">MSTSTPTSSTDSSDSEDSYTVPLHKPKFLKKSVFPNAEQGNGNPQQELKERIQQINERLDTLEYNGLSTAARGMSNEQHVLKLLKLDDSDADNVQEKRLWEERRAARIQAIKQVYIAKQLEIEQKEAQRLKREMHGTAASTTEHDEDQELWDFSKRSIRVSRVREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5A0</accession>
    <name>A0A1E5R5A0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein UIP5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3719</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R5A0"/>
    <dbReference type="FunCoup" id="A0A1E5R5A0">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5A0"/>
    <dbReference type="InParanoid" id="A0A1E5R5A0"/>
    <dbReference type="OrthoDB" id="270293at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005537">
        <property type="term" value="F:D-mannose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051136">
        <property type="entry name" value="Intracellular_Lectin-GPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223:SF45">
        <property type="entry name" value="RE50040P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223">
        <property type="entry name" value="VESICULAR MANNOSE-BINDING LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="639"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="686" mass="77742" checksum="A4785B16A4EF4165" modified="2017-01-18" version="1">MFSRKSSVVGMNEGLPIANPLSTRASIADLRSASSNAYNNDTNNNNNNNNNNSSSSINGPGMLSRKTSLGGGLNYPKPRQNTSQSFQSVNEASDDDYFQNGFNNNINSSSTSLGRSASTSVGAGPRLLKKTSSISDFGSRIMSQHKERQNDKYLEKEIAKFNTKYRMNSTNSSHSRIGSGIRKSSQRIASEVKELRPASLQKASKLKKLVVALLGFVLLYQFFIKPMGSLLGNGNASSSNNTNNFMFKSRNIDEIPLSERYLQERNQVSKFKNEKFKLSVPFLDTVYSNYDVGGHPIVKNKEFVKMISKGEVGKHSVMLSKEFLPSDFEMQISFVLEPGNHKIDETKRPGDGINFFFTQQSNFLEVDPTSYSQRQFMIQNDNIDPKNKDLMGVPKNLPAESLVLDFFQNGKITNPEGMQKWNDLTTPFLSFLSSTTEFAYDYKTDGVNNAHDIVKLSPSLLFTGKGEADVPKEIKLRIIKISNVLFKVDIDYTGFGLSWLEILRFEKFDKRFPNFSSNKIKFGISALNGDQTANFQIMKIDCFNYLWNNPTETNPDIKIENVMEMYRREYDILELDGWFDIKSLYRKSGLLPREIYDTDKMEYLKKKATNNANKPLDMGRVSNQQQQSASNGKVKKRSFFGKLLYCFWILLILSVVLYVVSLFLRVQKKKFKTRVSRTRVASDGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5B0</accession>
    <name>A0A1E5R5B0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amine oxidase</fullName>
            <ecNumber evidence="9">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3598</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="9">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <text evidence="8">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="8 9">Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the copper/topaquinone oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="9"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5B0"/>
    <dbReference type="InParanoid" id="A0A1E5R5B0"/>
    <dbReference type="OrthoDB" id="5379943at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008131">
        <property type="term" value="F:primary methylamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009308">
        <property type="term" value="P:amine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.20">
        <property type="entry name" value="Copper amine oxidase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000269">
        <property type="entry name" value="Cu_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015798">
        <property type="entry name" value="Cu_amine_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036460">
        <property type="entry name" value="Cu_amine_oxidase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016182">
        <property type="entry name" value="Cu_amine_oxidase_N-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638:SF33">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638">
        <property type="entry name" value="COPPER AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01179">
        <property type="entry name" value="Cu_amine_oxid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49998">
        <property type="entry name" value="Amine oxidase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54416">
        <property type="entry name" value="Amine oxidase N-terminal region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0186">Copper</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 9" id="KW-0801">TPQ</keyword>
    <feature type="domain" description="Copper amine oxidase catalytic" evidence="10">
        <location>
            <begin position="257"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="329"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="413"/>
        </location>
    </feature>
    <feature type="modified residue" description="2',4',5'-topaquinone" evidence="8">
        <location>
            <position position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ82090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="687" mass="78451" checksum="F267754F2B0AA3D0" modified="2017-01-18" version="1">MSSYCTPSVDIFNSITDDEIRITTSLIKKSCPNDNVQFIQVDRVDPPKKLATQLLNYVRYGKGTKPRIPRRTYAYFYKNNEMPLYKSLVNTTERHITCTCAVDAIGPLSPEFVNDCDEKVFDIPEVQAEIAKLKLDTQFYDHPQLGKLKYKILSEAWMYGISKEEDAEKPLCQVFMYLKLDHPNANHYSIPLTFSPVLEYLTAKYIRMDYLPNDALESFNPIVKPFIGKDIDHVPFEYHPDVVPNYKPAKGLKPLIVEQPEGPSFNVDSNNKITWQGWEFYVQSNVREGFAIYDVHFQGRSLFYRFALNEMCVPYSSKNETFSRKMAFDLGDCGFGNAANSLKLGCHCLGVIKYLDFRRSDREGNPVVNPSTICMHEQDYGILFLHQNYRSGASTTTRRREFVVQTIATVANYEYIVNFVFDQGGAINVQVRATGILSTTPLNTEVTSTDFGTIIAPGVHAPFHQHLLSFKFDPRLDGDNNSVCYDDYVPNEEDPEKWNVGYVQKRTFMEKSGHIDQSPFTNRTYKVINEHSINPTTKKPVAYKFEMPAKQMIMAGKNSYHLKRAHFATQQFWVHKYDDERRYAAGEFTNQSRKDTGISKWCDGTESVRDEELTVQVTLALTHPPSSEQFPLMSSDFMQFAVSPSSFYXKNPALNVPLATNKFNQSVYYEDSAKTCSNAAGSCCSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5J0</accession>
    <name>A0A1E5R5J0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate reductase</fullName>
            <ecNumber evidence="4">1.1.1.302</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g3729</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes an early step in riboflavin biosynthesis, the NADPH-dependent reduction of the ribose side chain of 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate, yielding 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>2,5-diamino-6-(1-D-ribitylamino)pyrimidin-4(3H)-one 5'-phosphate + NAD(+) = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27274"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58890"/>
            <dbReference type="ChEBI" id="CHEBI:59545"/>
            <dbReference type="EC" id="1.1.1.302"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2,5-diamino-6-(1-D-ribitylamino)pyrimidin-4(3H)-one 5'-phosphate + NADP(+) = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27278"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58890"/>
            <dbReference type="ChEBI" id="CHEBI:59545"/>
            <dbReference type="EC" id="1.1.1.302"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; riboflavin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HTP reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.302" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R5J0"/>
    <dbReference type="FunCoup" id="A0A1E5R5J0">
        <property type="interactions" value="105"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5J0"/>
    <dbReference type="InParanoid" id="A0A1E5R5J0"/>
    <dbReference type="OrthoDB" id="5432at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="entry name" value="Dihydrofolate Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050765">
        <property type="entry name" value="Riboflavin_Biosynth_HTPR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38011:SF7">
        <property type="entry name" value="2,5-DIAMINO-6-RIBOSYLAMINO-4(3H)-PYRIMIDINONE 5'-PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38011">
        <property type="entry name" value="DIHYDROFOLATE REDUCTASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_8G06820)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="Dihydrofolate reductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0686">Riboflavin biosynthesis</keyword>
    <feature type="domain" description="Bacterial bifunctional deaminase-reductase C-terminal" evidence="13">
        <location>
            <begin position="19"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ82138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="250" mass="28128" checksum="FCC9D1923FBEB96E" modified="2017-01-18" version="1">MLQYEANLKNYIPPSLNTRHVTLTWAQSLDSKISQGHGIRTTLSHPETKEMTQYLRSKHQGILVGSGTVLSDDPGLNCKKSIHHSPRPIIVDPSFKFLHKFENSKLRSLYLMKLGKPPIFVITEEAHKLHIKYTEERTILGEFDILIAPTCEKTKVIPWKNILDELDTKFGIKSIMIEGGAYVINDLLSLYPHHINSVVVTVAPVFLGDQGVTVHPSMGKIDLENVTNFTGLTDVVMCGQIKQNLQTKDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7E0</accession>
    <name>A0A1E5R7E0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">High mobility group protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3137</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7E0"/>
    <dbReference type="FunCoup" id="A0A1E5R7E0">
        <property type="interactions" value="297"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7E0"/>
    <dbReference type="InParanoid" id="A0A1E5R7E0"/>
    <dbReference type="OrthoDB" id="3972868at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF24">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="4">
        <location>
            <begin position="100"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="2">
        <location>
            <begin position="100"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="215"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="239"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="5">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ82798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="255" mass="28400" checksum="8BC097503E351023" modified="2017-01-18" version="1">MSEQSLKFRVAKDSASSQTASCVVDFYNCIGEDEDLKNDAFMTLTEALKKLTGSATQLQEVAVDYSGALPVATAPSIAEVVNEPKKEEVKKRASKDPFAPKKPLTVFFAYSAYIRDSLIEDRKKSNLPPYTSVEITQEISKRWKELSDEEKSKWKQAYMAELEHYQKRKAEYLEAKKNGTFQADVDFTNNAPVPIPDDIGVTSQAIEDAVIAELENVDSQDAGASAGSKRTRASEPEKKKKKKKSKKDKKNASNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7F5</accession>
    <name>A0A1E5R7F5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">NET1-associated nuclear protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3373</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7F5"/>
    <dbReference type="FunCoup" id="A0A1E5R7F5">
        <property type="interactions" value="447"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7F5"/>
    <dbReference type="InParanoid" id="A0A1E5R7F5"/>
    <dbReference type="OrthoDB" id="4096at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000234">
        <property type="term" value="P:positive regulation of rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057644">
        <property type="entry name" value="Beta-prop_WDR75_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053826">
        <property type="entry name" value="WDR75"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44215">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 75"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44215:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 75"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23869">
        <property type="entry name" value="Beta-prop_WDR75_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23769">
        <property type="entry name" value="Beta-prop_WDR75_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="302"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="WD repeat-containing protein 75 second beta-propeller" evidence="9">
        <location>
            <begin position="452"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ82839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="910" mass="102809" checksum="9641E34438034C46" modified="2017-01-18" version="1">MSDSNKYSLSVVSGGRLRFPRAFELTYSINKCSNNHNGYLSKDCRHYIVPFNNMLKVYSLETRQCVKTFKYLNNQILQNIFDNNAKRDIEVVHIDFEVTDLNEERLVLYTNYFQKIDLKYRGKLEYSEPLKVEQLRKPEGASKLIKVFANALLVETPDSLQIFANSFGQETENTTFEPLRSFPLNTISKYCWSNNEQQLCVISQIFINDDSKKESKKANRNKHKKKNANGQLKKITTLNLDNLKETMDYTISNTTQSQSVNATIISSVAINNEADQVAIGFASGVINLLTLSNDEPPSTRLMKWHIDSVVSLAFEQSGQYLISGGWEKVLCFWSLKNSNTQQFLPRLNGVVVNVTSLNIPGYHSSTQNGLISVMMQYADNETMSDYQIIVVDTSDLQSKLSCNGPLAMFENNSQYRKPISAINTGGMLKHKCNAAFQQNGLKKKDVTIKPFVIEPTSKNLLYFPHAAGMCVYDLNKNEQLKYQNLSFNVTSSMGKVRYEHENIVEPQIQQLKFNSDGNWLVSYEIQEQPQGLISSKDTQYALKFWNYNQTTNDWVLQTKISNPHGEKTPVVEIAPHLTRKNCFVTCDNQGGLKMWGIVDEKFMRWGLIDMKLASSNSQSSFISVCFSKDGSLIFQSFNDKLYIMDSSNFNKVGDQNFLQLDSNIQSMVLTSNNENLIICTMTSILCYNLLLGESTTGYDIYSYLNTENGASSTKATSATPLYRPGNMKKLLAIDEKTSNVAVVFNMSNNTSKVMIFNESLTEIISTFDYEENISCINWNHDDTFVFVDAQSRIGVITAVGLQGFALDENSAAKYYNGLNNESKHSMGTRIKTDMNEEEFNRDLKVLSKKASDFVKKTASSKNSSVDEEDLVSGAKASKTLNSNTFMGLFDTIENVQMETLFDSVAKILSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7H6</accession>
    <name>A0A1E5R7H6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein IVY1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3206</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7H6"/>
    <dbReference type="InParanoid" id="A0A1E5R7H6"/>
    <dbReference type="OrthoDB" id="5594612at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037470">
        <property type="entry name" value="IVY1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38407">
        <property type="entry name" value="PROTEIN IVY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38407:SF1">
        <property type="entry name" value="PROTEIN IVY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="160"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="233" mass="26289" checksum="A43B4546659DCF4C" modified="2017-01-18" version="1">MNQKKNHKNLLNYRENIYELENRLSNIENLKIDYYHGSAQLLNEHSKYVLQQVASLIAIQLELHEEMAKKGWSGGGLEFVHRMKNLGEDEEEEFLSNEEQIKDTSASMPIKETQTILSSPKKQTPQESLYQVTQQQENKKSPLTNKASMEQEPNTSINTSMSSGKSVXSNRSSQSGRTIRMTSIAYKNSPDTNTQGEXAAEDAKPPSIDGANEDETSFSLPQMENDFTDQSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7L7</accession>
    <name>A0A1E5R7L7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing factor CWC2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g3223</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM CWC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7L7">
        <property type="interactions" value="223"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7L7"/>
    <dbReference type="InParanoid" id="A0A1E5R7L7"/>
    <dbReference type="OrthoDB" id="10251848at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036002">
        <property type="term" value="F:pre-mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039171">
        <property type="entry name" value="Cwc2/Slt11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032297">
        <property type="entry name" value="Torus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089:SF2">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16131">
        <property type="entry name" value="Torus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="8 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="16">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="15">
        <location>
            <begin position="141"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="13">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="270"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="275"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ82905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="353" mass="41101" checksum="D2B7BEE80FC1C30D" modified="2017-01-18" version="1">MMNRPARVQVAEDGLPSNLPPQNGLVFNVWYNKWSHGTNNRNNQRFLNRYRCDPIRDTGYTRADKLKQTNSLKNNGXTKKSLXYHCLXFAKGICCKGSKCEYFHHVPTNEVSVVYDCFGREKYSDFRDDMGGIGSFQKQNKTLYVSGLQQGSGLPSNMSSAAVEQRVKARFQQFGDIEYIRYLKDKHCFFIKYRFQSCAEFAKEAMSHQTLLTEQEMNENEQLEQDVLGIIVKWAKDDPNPHVQKLEREILKKNALEKMAELFSKTSQKKRLYDENDGDSDDETENNDDAKRKNNDNVNFNKNKKQKFMNSQIEQSQHLFKDSDIKVFQKKVLKKRSTRPPMQALLAEVYNSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R842</accession>
    <name>A0A1E5R842_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Urea amidolyase</fullName>
            <ecNumber evidence="11">3.5.1.54</ecNumber>
            <ecNumber evidence="12">6.3.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g3282</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>urea + hydrogencarbonate + ATP = urea-1-carboxylate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20896"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15832"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>urea-1-carboxylate + H2O + 3 H(+) = 2 NH4(+) + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:19029"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15832"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.1.54"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (allophanate route): step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (allophanate route): step 2/2.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.54" evidence="11"/>
    <dbReference type="EC" id="6.3.4.6" evidence="12"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R842">
        <property type="interactions" value="183"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R842"/>
    <dbReference type="InParanoid" id="A0A1E5R842"/>
    <dbReference type="OrthoDB" id="196847at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004039">
        <property type="term" value="F:allophanate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004847">
        <property type="term" value="F:urea carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000003">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000010">
        <property type="entry name" value="Propionyl-CoA carboxylase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.10:FF:000010">
        <property type="entry name" value="Urea amidolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.10">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053844">
        <property type="entry name" value="AH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014085">
        <property type="entry name" value="Allophanate_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050856">
        <property type="entry name" value="Biotin_carboxylase_complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003778">
        <property type="entry name" value="CT_A_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003833">
        <property type="entry name" value="CT_C_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014084">
        <property type="entry name" value="Urea_COase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02713">
        <property type="entry name" value="allophanate_hyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006043">
        <property type="entry name" value="PRK08186.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00724">
        <property type="entry name" value="urea_amlyse_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02712">
        <property type="entry name" value="urea_carbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18866">
        <property type="entry name" value="CARBOXYLASE:PYRUVATE/ACETYL-COA/PROPIONYL-COA CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18866:SF128">
        <property type="entry name" value="UREA AMIDOLYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21986">
        <property type="entry name" value="AH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02626">
        <property type="entry name" value="CT_A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02682">
        <property type="entry name" value="CT_C_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00796">
        <property type="entry name" value="AHS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00797">
        <property type="entry name" value="AHS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160467">
        <property type="entry name" value="PH0987 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0092">Biotin</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="18" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="17">
        <location>
            <begin position="648"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="16">
        <location>
            <begin position="767"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="15">
        <location>
            <begin position="1775"/>
            <end position="1853"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00052931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00066801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ83079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1853" mass="204037" checksum="46578DB2FC794160" modified="2017-01-18" version="1">MSNWGITEWVEFHKKSTKAESFQKLSELVKSQKIAPADPAWISVIDDSTLSKQLDYLYTLPNYSNLPLFGVPIAVKDNIDFEYLPTTAACPSFLYTPEKDSTVVSLLRKAGAIVIGKTNLDQFATGLVGCRSPYGKTPCVFSDKHVSGGSSAGSGSVVGRGIVPIALGTDTAGSGRVPAALNNLIGLKPTKGVFSCSGVVPACKSLDCVSIFAMNLVDAEKCFKIMAKFDGSQDEYSRHFPETPMQKFSAKPTIAIPQNCPWYGETENPKLFAHSVSVLKQTGAEVIEIDFTPFLELAKCLYEGAWVAERYAAVKDFYSTNPPEKDLDPVVTGIIKGATNYDATAAFNFEYKRQGIQQKISKILETQKIDLLCVPTCPLNPTFADLEKEPVKVNSYQGTWTNFVNLADMAALAIPTGFRSTDSLPQGITLIGPKFSDFALLELASRFFKINYPENSRTSGKFLNQPVGHRDTEITGPELNLDACVQIAVVGAHLKGLPLHWQLEACNAFFVKSIKTASNYKLYALPKVGPVLKPGLKRVVDSKVEGDGNNIDLEIYAIPAEFVGKFISYIPEPLGIGSVELENGEWVKCFICEEFGYAQKGSVDITKLGGFKPYIQQLKAEEAKKTTISKVVEVPHERESEHSHLPKPFDTVLVANRGEIAVRIMKTLKKLGIKSVAVYSDPDKYAEHATVADVAVPLHGTTAAQTYLDVEKIIAAAKETNSQAIIPGYGFLSENADFSDRCGREGIVFVGPSGEAIRKLGLKHSAREIAEKANVPLVPGSDLISSAKEAREVAATIGYPIMVKSSAGGGGIGLQKVDDEKDIERVFETVSHQGKAYFGDSSVFLERYVENSRHVEIQMLGDGFGKAKAIGERDCSLQRRNQKIIEETPAPNLPDATRAKMRQASESLGSLLKYKCAGTVEFIYDQKRDEFYFLEVNARLQVEHPITEQVTNLDLVEWMLRIAANNAPSLDTIDITPRGASMEARLYAENPVKDFRPSPGQLTHVTFPKWARIDGWVKKGTKVSAEYDPTLAKIIVHGNDRADCLAKLNEALNETSVYGCITNIDYLRSIASSEMFADAKMHTKILDSYNYQPSAFEITSPGAYTTVQDYPGRVAHWRIGVPPSGPMDAYSFRLANRIVGNHYKAPAIEVTLTGPKVLFHTNTVIAITGGTAPCTLNETPIEQNKPIYVRRGDLLSIGKLSTGCRSYLAVRNGIDVPEYLGSRSTFALGNMGGYNGRVLKLGDVLFLNQPELESATLPGPVFEPKVLPNGLIPNIPAGKEWTIGVTCGPHGSPDFFKPESVEEFFSEQWKVHYNSNRFGIRLIGPKPKWARQNGGEGGLHPSNQHDYVYSLGAVNFTGDEPVIVTADGPSLGGFVCQAVVPEGELWKVGQVKPGDMIKFVPVSYDAARTIKESQDAAIESLDESKLVLLNEKLILPSYEDPVLKVISGSSEFSPKVTFRQAGDRYILVEYGELEMNLNFAYRINRLINKVAEMKTFGIVEMSQGVRSVLIEFDGYKITQSELLDLLNQYESEIIFDGXWSIKTKAFKLPVAFEDSKTLDCVKRYQETIRSKAPWLPNNVDFIADVNGVTKNDVKDMLYSAKFMVLGLGDVFLGAPCAVPLDPRHRFLGSKYNPSRTYTPNGTVGVGGMYMCIYAMDSPGGYQLVGRTIPIWDKLMLGEHSKEHPWLLTPFDTVEFYLVSEKELNQYTEDCENGVFKVLYEESEFDHKNYTAWIEENKESIEAFKKTQDGEKAEEFANLIAVANQELEENAAEKVEXQEEYPENAEMVYSEYSGRFWKSIVAEGDAVKKGDGLIVVEAMKTEMAVASPATGKVFKILHKNGDMVEAGDLVAVIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R888</accession>
    <name>A0A1E5R888_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome assembly protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3192</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for efficient biogenesis of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RSA3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R888"/>
    <dbReference type="FunCoup" id="A0A1E5R888">
        <property type="interactions" value="295"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R888"/>
    <dbReference type="InParanoid" id="A0A1E5R888"/>
    <dbReference type="OrthoDB" id="69550at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051898">
        <property type="entry name" value="Ribosome_Assembly_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028217">
        <property type="entry name" value="Rsa3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28127">
        <property type="entry name" value="RIBOSOME ASSEMBLY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28127:SF1">
        <property type="entry name" value="RIBOSOME ASSEMBLY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14615">
        <property type="entry name" value="Rsa3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Ribosome-assembly protein 3 C-terminal" evidence="9">
        <location>
            <begin position="143"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="70"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ83081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="197" mass="21959" checksum="821426F57EA761B2" modified="2017-01-18" version="1">MSDITNGSKPKSSKKNNIKRRRNKKRRTADVTSSEDSDASSDVSLGTDSDKEDNTTTGQQSVSLQEEDADIKLSDTEADQDKNMDNAADVEMADAQYEGRKRLNEIKLTNSLKEINGPAHSNTQTLGDYNKASQNLDNLDKDLKTKFLQMTFEHYGEDVNQLRDASDFTNKSLVILANALKEGARMFDNDSLKSILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R8Y6</accession>
    <name>A0A1E5R8Y6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sidoreflexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3465</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the sideroflexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R8Y6"/>
    <dbReference type="FunCoup" id="A0A1E5R8Y6">
        <property type="interactions" value="286"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R8Y6"/>
    <dbReference type="InParanoid" id="A0A1E5R8Y6"/>
    <dbReference type="OrthoDB" id="6608471at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015075">
        <property type="term" value="F:monoatomic ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990542">
        <property type="term" value="P:mitochondrial transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004686">
        <property type="entry name" value="Mtc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00798">
        <property type="entry name" value="mtc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153">
        <property type="entry name" value="SIDEROFLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153:SF6">
        <property type="entry name" value="SIDEROFLEXIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03820">
        <property type="entry name" value="SFXNs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ82993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="329" mass="35759" checksum="A43DBA4F2ED625AB" modified="2017-01-18" version="1">MASSVTGPVPLPHSRYDLSTYWGRVKHCAEVSDPRELLNTDKDLDAAKKVISDYRNGVLKTTTPEFWEAKKKLDSTIHPDTGEKVLLPFRMSCCVMSNLVVCVGMMTPGLGTMGTMFWQWANQSLNVAINSANSNKSHKMSTSTLITNYTAAVTASCGVAVGLNKLIPKLKNLSPNTKLILGRLVPFAAVVSAGIVNVFLMRGEEIRKGINVFDENGDQVGVSKKAAVVAVGETAASRVINATPIMVVPPLLMVRLQKTILKNKSFMVQQLASLGIITLTSFVALPFALAAFPQHEAIALKHLEPELSNKQLTTKDGKKIETVYFNRGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA17</accession>
    <name>A0A1E5RA17_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Trafficking protein particle complex subunit BET3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2532</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Golgi apparatus</location>
            <location evidence="1 8">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RA17"/>
    <dbReference type="FunCoup" id="A0A1E5RA17">
        <property type="interactions" value="1047"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA17"/>
    <dbReference type="InParanoid" id="A0A1E5RA17"/>
    <dbReference type="OrthoDB" id="10262857at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14942">
        <property type="entry name" value="TRAPPC3_bet3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1380.20:FF:000001">
        <property type="entry name" value="Trafficking protein particle complex subunit BET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016721">
        <property type="entry name" value="Bet3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13048">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018293">
        <property type="entry name" value="TRAPP_I_complex_Bet3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7 8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR018293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ83727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="203" mass="22860" checksum="ACB17A89C891430F" modified="2017-01-18" version="1">MDKAKQYRAIGDDIWANRTLKINAELLALTYGSIVGQLTREYKNDFTKINEKLRAMGYNIGVRLVEDFYARSAMGRCRTIEETGLVLTQVAFKMFLNVTPQIQVINETELHIFLNENPLSAFVELPPFDEDDLEVDKSGDGSGKSSQLWYSNILCGVIKGALEQVAIDCEVEFVRDVLLGDHTTELRISGFKFLKDEVPAGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA67</accession>
    <name>A0A1E5RA67_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sensitive to high expression protein 9, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2648</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Required for the maintenance of the structure of the mitochondrial inner membrane. Involved in mitochondrial morphology. Causes growth arrest when highly overexpressed.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homooligomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Mitochondrion inner membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the SHE9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA67">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RA67"/>
    <dbReference type="OrthoDB" id="5595506at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007007">
        <property type="term" value="P:inner mitochondrial membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008839">
        <property type="entry name" value="MDM33_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31961">
        <property type="entry name" value="SENSITIVE TO HIGH EXPRESSION PROTEIN 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31961:SF3">
        <property type="entry name" value="SENSITIVE TO HIGH EXPRESSION PROTEIN 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05546">
        <property type="entry name" value="She9_MDM33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="70"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ83792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="480" mass="55772" checksum="1547D400239E7363" modified="2017-01-18" version="1">MTVHGMKVLKCFYPKGLFHSSFAIVASKSRGQGIAFQHGDAAFRLCIVGRRCYATDSSGKERSSENKGILEEIRIEAAKISSKLGKYNQQFKETIEKAQLKIQEANAKIKLQENEANKIKYDTDLSTHKAIDNLPSRMELRRTQFARKLEFYFDSLQETIFTATKALNDVTGYSAIQKLRNSIKTMEDQLREQRLSLKRVKTEYERVVKHKTESQNEINALLQRKSSWDPADLEHFTKIYRENQNNVKLEIEKSQELKXMEKKTESLENDLYNAILTRYHEEQVWSDKIRRTSTWGTFLLMGVNILLFVVVQIVFEPWKRRRLVRAFEDKVKVALEDYKTDQDEKFDKLLKLLEQNEAAQNIVEKHLGINESLLEETEEPVSHKSPELREKNDAHVQQDIGSAEVVQNNENGLKDASKISTNQSLKHQLVISYQRLKSHAQSLYDNKLYIPSLASSVQSTLLHFWHQIRFLLGIEYINSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAB8</accession>
    <name>A0A1E5RAB8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein phosphatase PP2A regulatory subunit B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2821</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the phosphatase 2A regulatory subunit B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAB8">
        <property type="interactions" value="816"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAB8"/>
    <dbReference type="InParanoid" id="A0A1E5RAB8"/>
    <dbReference type="OrthoDB" id="6274823at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000009">
        <property type="entry name" value="PP2A_PR55"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018067">
        <property type="entry name" value="PP2A_PR55_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11871">
        <property type="entry name" value="PROTEIN PHOSPHATASE PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037309">
        <property type="entry name" value="PP2A_PR55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00600">
        <property type="entry name" value="PP2APR55"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01024">
        <property type="entry name" value="PR55_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="396"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="465"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="402"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU331113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ83837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="538" mass="60296" checksum="D5975940A51AEC93" modified="2017-01-18" version="1">MSLDNYDFKFSQCFGDKADIVVTEADIITSVEFDHTGDYLATGDRGGRVVLFERNLNSKKHCEYKFMTEFQSHDAEFDYLKSLEIEEKINQIKWCKYTNNSHFLLSTNDKTIKLWKIYEKNIKLVSENNLSTGLFNGGSDANIINNNNSNNXNNINGGTDIKKLSALKLPRLTNHDKIVAASPKRIYSNAHTYHINSISPNSDGETFLSCDDLRINLWSLDIPDQSFNIVDIKPANMEELTEVITSAEFHPSHCNYFMYASSKGTIKLCDMRTNALCDNYQAKVFEEYTDPSTHNFFTEITSSISDVKFSPCGRYIASRDYLTVKIWDINMNNKPLQTLHIHDQLKDKLSDTYENDAIFDKFEVGWQGNSKSVMTGSYNNNFMIYPNVLGSDTSKLNVPPGSASSSSSTNSNTTTSITTNGSTHSITDHTDQEYDYKNVIKLQADKNAFKSASNNINMNVQNGSNGNGYWGSEDNGSSSRNISDGAGAGGLHGHGHGQQMALQEEIDYKKSILHFSWHPXENSVAIAATNNLFIFSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAD4</accession>
    <name>A0A1E5RAD4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein SAP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2763</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAD4">
        <property type="interactions" value="88"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAD4"/>
    <dbReference type="InParanoid" id="A0A1E5RAD4"/>
    <dbReference type="OrthoDB" id="10251136at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19509">
        <property type="entry name" value="RecA-like_VPS4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000022">
        <property type="entry name" value="Fidgetin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF17">
        <property type="entry name" value="FIDGETIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="725"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="106"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="400"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="827"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="128"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="143"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="184"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="196"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="237"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="256"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="303"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="407"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="431"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="466"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="488"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="513"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="547"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="560"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="845"/>
            <end position="856"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ83858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1007" mass="111122" checksum="1163392BFC239FDE" modified="2017-01-18" version="1">MDLDHNLSVLTKFTKFRKKPQQPLTDLTELYNRVSHESIYYKRLSEKGELEKAIQGWKIISTHVLYDLQQLEKKYPNKHNYNEDENSLIAGIRKFYMTTVENLEKYNTDSRQRSNKSKPAQPPMVKTSGFTKTIKSSNSNPANSRLLNNRMLRTLRPNTSRASVSPSSSNPSSRSGSFALGVPTASTLSESTETSKPTQLRSTPTGAPPIPSVPRKLRIEKSQSLTSAGSEYETATEAEKPRKSTSDIADRPHSPLLNTNKLLSNYSPRTTFIDDHRNTSRGGSNRSHSIEVIDLTEDNDDTSDSREQHELSSFDDEKPENPFLVAEPGSPIDFNIDDYLYSGESEKEELDSEEETYKKANGGINVDVSEKDIEQLLAKSNFAPPDFSRSSIIVQESKLPRAENHKSASIPNPPLTSKINTTLRLPKKNPKSQTELEGRSNSNEPGASHTKPEPSNVFPRLKHPSSTRSTPSLPTQSTVLRKKAASQAGKTFSSTVTKTPATSASRKARSKLPSRPSGNSSTSSTMSIGTQKAIAASAKVSLEAVRHAKPSTKKSHXHRNNQQPVLKTRKSANVLNQFSSPASTTLTSRSNSTALDKKSKSEVDLSKTNAIASSISTNKKPVSRNNSPRIPAITVFQGDTSAAGNSTVDVTTEPDLKTQLEEEILDSIPGIDKLAGRQIFQEIVVQGDEVHWEDIVGLETCKNSLKEAVVYPFLRPDLFKGLREPIRGMLLFGPPGTGKTMLARAVATESKSTFFSISASSLTSKYMGESEKLVRALFQIAKKLSPSIIFVDEIDSILGSRNGDGEHESSRRIKNEFLIQWSGLSSSTAGSSDADNKNKEAVHNQGVSKTQSNTPNEEVDDRVLVLAATNLPWSIDEAARRRFVRRQYIPLPESETRVLQIKKLMKFTKNVMFAPMADEDSEIHTVTLLAEWTQGYSGSDITSLCKDAAMGPLRELGDKLLFTPKNDIRPVSLXDFENSLKYIKPSVSVDGLKEYDDWARQFGSSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAH6</accession>
    <name>A0A1E5RAH6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thiosulfate sulfurtransferase RDL2, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2781</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAH6"/>
    <dbReference type="FunCoup" id="A0A1E5RAH6">
        <property type="interactions" value="568"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAH6"/>
    <dbReference type="InParanoid" id="A0A1E5RAH6"/>
    <dbReference type="OrthoDB" id="566238at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE RDL2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086:SF10">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE_RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="72"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="174" mass="19670" checksum="AE80B409E5B198ED" modified="2017-01-18" version="1">MFVRSIRLNSSLLLSAAKKPQPALSKLRLTTLPTTYMQQQKMGQSTSSEQNPSSQFKSYKYEDILKLVNEEPEQGPYLIDVREPKELAELGKIPNALNIPYKTAPGSLDLKPAEFQELYSFEKPDENKELVFYCAAGIRARAAAELAQSFGYKHCSVYPGSFNEWMIRDGPIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAH7</accession>
    <name>A0A1E5RAH7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NADP-dependent 3-hydroxy acid dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2776</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAH7">
        <property type="interactions" value="535"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAH7"/>
    <dbReference type="InParanoid" id="A0A1E5RAH7"/>
    <dbReference type="OrthoDB" id="6251714at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000047">
        <property type="entry name" value="NADP-dependent L-serine/L-allo-threonine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42901">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42901:SF1">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ83904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="267" mass="29233" checksum="5AEED03401159455" modified="2017-01-18" version="1">MSQGSKAAERLANKTILITGASAGIGQATAIEYCAASNGNVKLILLARRLEKLNELKDTITKDYPNAKVFTAQFDVTEVAKVEPFLESLPAEFSDIDILVNNAGKALGSDKVGSIAEEDVEGMFETNVIAMINFTQKILPKFLAKNKGDIVQLGSVAGREAYPTGAIYCATKHALRAFTQSLRKELINTKIRVFEIAPGNVETEFSLVRYKGDTERANQVYKGTTPLYADDIADLIVYSTSRKENVVLXESLIFATNQASPFHIYRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAK9</accession>
    <name>A0A1E5RAK9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Helper of Tim protein 13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2491</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAK9"/>
    <dbReference type="FunCoup" id="A0A1E5RAK9">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAK9"/>
    <dbReference type="InParanoid" id="A0A1E5RAK9"/>
    <dbReference type="OrthoDB" id="411372at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016694">
        <property type="entry name" value="UCP017292"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037274">
        <property type="entry name" value="Znf_CHY_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017292">
        <property type="entry name" value="UCP017292_Znf_CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161219">
        <property type="entry name" value="CHY zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ83934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="118" mass="13769" checksum="0136804BF4D6FE3B" modified="2017-01-18" version="1">MSELIKGLLVDDESRCEHWHSELDVVALKFKCCPEVYYCCYSCHLELNDIDPETGSSSGGKHQIEKFSRNDNTVKLIMCGACKTEMTFAEYTKDMKCYNCKHNFNPRCKIHYDLYFTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAR6</accession>
    <name>A0A1E5RAR6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pheromone-regulated membrane protein 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3134</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ThrE exporter (TC 2.A.79) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RAR6"/>
    <dbReference type="OrthoDB" id="3971426at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010619">
        <property type="entry name" value="ThrE-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051361">
        <property type="entry name" value="ThrE/Ser_Exporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024528">
        <property type="entry name" value="ThrE_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31082">
        <property type="entry name" value="PHEROMONE-REGULATED MEMBRANE PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31082:SF4">
        <property type="entry name" value="PHEROMONE-REGULATED MEMBRANE PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06738">
        <property type="entry name" value="ThrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12821">
        <property type="entry name" value="ThrE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="574"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="600"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="633"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="666"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="731"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="755"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="781"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="836"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="domain" description="Threonine/serine exporter-like N-terminal" evidence="9">
        <location>
            <begin position="444"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="Threonine/Serine exporter ThrE" evidence="10">
        <location>
            <begin position="711"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="190"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="202"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="252"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="312"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="327"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ83996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="867" mass="96431" checksum="334106FC440DFDAF" modified="2017-01-18" version="1">MLPSFNKNERDSNSDSVNAQNVARHIDQDSQSIHTHGSFAPGENKTNDASNEEPIHLNDMNLQPTENQTNNATQELSPDLEQNMAPEHHEMEDHEFYPEHEEDPQRHAEMLEHLDDMGVVPNYAGIGFGMVPSFLDNNSEIQKYREDIRNDLEDGEDSHRFDIDFTDSATLNSDSDEDQDHDDNYVEGYYPEQEEHGSNAPRRNSSIRSTRSNRSTHSNRSTQSNRSNRSNRSNRSSRSQKRGKVDHRSPLRRLGILGSLLRLHQATPVTRSNTHVRRNDKNRKSSSYSSSSTDNDAEANQELLEKSKKXTDEEEENEDKFDTDQATMTNTRTNTNTINQSTPQXKPPLVSKSSSXFNGMKNSNMYVKGKNVLPSSMKTAKAAGQGVYSAGQGVYNTGKENMPNFKSVRPKLNNASKLNKLIKKRKAHEAKITVHITNLLQKHRFIIRLCKAFMLYGAPTHRLEEYMKLTSKVLECDGQFLYLPGCMVISFGDVTTRTSELQMVKCDQGLNFWKLHQTHKIYKDVIHDIISVEEANNDLDDIMASPDLYGTYITVPLYALASGIITPMAFGGSWVNIAISFVVGGCVGLLKFILSPKSQLYSNVFEITSSIVVSFLSRALGSIPHSNICFGTVVQGSLALILPGYIILCGSLELQNKNLVAGSVRMFYSIIYSLFLSFGITLGAALWGWMYHNATNETTCTKNVSDYNKIWFVPAYTIITSFINQANWTQIPVMTVIACTGYIVTYYSGKHFSNATEFTSAMAAFVIGILGNLYSRIWKGLAVSAILPAIFVQVPSGIASKSSLLTGVNTANTIVNQSGTVVTETSTNSLSFGLTMVQVSIGITVGLFASTLVIYPFGKKKTAIFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAR8</accession>
    <name>A0A1E5RAR8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2817</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RAR8"/>
    <dbReference type="OrthoDB" id="416217at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="665" mass="75352" checksum="63A6774964AA401C" modified="2017-01-18" version="1">MLKKSGKAXAPNALPLSPQMSENVNSELLENNGSSQTHIAASNGSFAPSQEPSVRQNNIQSLDSDSSPNHMHXHSAQLTSILNDVNHFTTELTNDINDKFSADNSDSTGNASEDTNNSESKKLETSETYLDDRLVEAPDRSYSDDFRNIFDTFTNGDAAQHAXRPSIIHESTSWSELRNKFEAIGLPIHEIFYLRVFYEKASYWVIPFAENPESNVANNVLFYHITSGPLSIASFSASCLAPALIAIAAKYTYNVTGEAKHKXHRDNYLKSALLNLANEFDYNRNTETLMAQKIESLILTILLLAMFSSSTNIDWKVHLQGAKDIFLRYSKIVEKDYKREETLALSKVWFVGLDGIAHLLIDHGGSSSSDEEFDTLADFVSDPXAKEVLVNMKISTPNHFNLLLGCSTDLLLFDKSLVRYISLGVGTFREKDPYNKNFCQTMYYAEVSRRFVFYEHEFGKVLKQNIKPEALSSKAIFYKAASDEYYSFFDISQQVHVELSLLIYFVHVGLGNTDQFKCSFFSMRRYMQFMFAVSTKGHRSSSDKDESICDVFESSHHLVIDEISRRELNSSDVLFTKYDFRSGLDKQLRGDFRLMMFLTSVLIFGKHIDECIKDVYASKNEKCKIIAFYQSLYQDCGCESAKSSLDVLISSWQNIEHYPNTIPFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAU3</accession>
    <name>A0A1E5RAU3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2911</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAU3">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RAU3"/>
    <dbReference type="OrthoDB" id="3972467at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="448"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="492"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="714"/>
            <end position="742"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="745" mass="84791" checksum="ED5EDCD4200D37B4" modified="2017-01-18" version="1">MTRLGYMSSFYMPLLCSSRRVVAGSMILLIVMIYFAMYLSSSLLISQYSETEQQSSTLSTXRGLRFFLSRVLTFNSDGNNDADLNPGQLFAPNSQDYFRTVLLGIFPPLLLNFVKNFFLNWKTSSKSYEIILSLFSDFVIHDWFLFLIIFFLAYPQVDSTNYHSNSKLQTEENGVPNVWHIIPKQSYIFGISWSLSEFLISILENISYYQEIPVPAEHLNEVEIVDASSKTGDKPKRAVXSVLVSKPGSQSLSKNSSFFSPNSLLSPDHSPDPYXKTHKLSLRNWINLDACTNALKSATLLSSKKQHDDENEDENTALNSNALAAQNVGSLNKESDSSKLSKQNITSTGETEDVLYVDLNNNFMGYDSILNSNKYTDDPTVYKNNGLSTLPQKLSRRFSDWTRTDPGLAAMSNDESVNNVNTNNGQYPTRFFYRIENSKKVWTKLFQCFLIILGDVLAVIGQSLISSIYFIYVPGHEQLFTPFVNYWGAKNIYFFLLTVMVPFTIISFIFHFLIYFWFSLKEEYKLLNKDFDAYFPDISGSGYYDGMSADYMGGQANNLFNQSYEPYDYYQNVINDIQKQQQQQQQQQQQKTGPYENMYMAGNNNASNSNNLRNALQYNANINTKTNNTLLPNSSLDYFLASSGSNNNQMRPLLSSNPYDLFNTHRNLSIATGADMGIGVSENFFKRFLAKLNILQIFKKTLYQWRKKTLKPNVIITASCLYGVVLFTLGVAATITGAYVYFDNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB63</accession>
    <name>A0A1E5RB63_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative pathogenesis-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3098</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RB63"/>
    <dbReference type="STRING" id="56408.A0A1E5RB63"/>
    <dbReference type="InParanoid" id="A0A1E5RB63"/>
    <dbReference type="OrthoDB" id="337038at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP" evidence="1">
        <location>
            <begin position="39"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="184" mass="20815" checksum="4BB3ADA9B810F9BC" modified="2017-01-18" version="1">MPCAKTSYTTSVLTEYSTKTVPYVVAPTYASTNIVEELDFQKKALKAHNVLRVKHGAQKLRWSAELQKQAQVFLETEFVCNGTLQHTNAFKAGEIGESVALGYASIEQAVQAFSHGQKYYSFANSTCVDKRAHQFTQLVWNNTEYVGCAYINCGNYYNNFFICQYTQKGNIPNEYPENVFQVLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB95</accession>
    <name>A0A1E5RB95_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Inosine triphosphate pyrophosphatase</fullName>
            <shortName evidence="10">ITPase</shortName>
            <shortName evidence="10">Inosine triphosphatase</shortName>
            <ecNumber evidence="10">3.6.1.66</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Non-canonical purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Non-standard purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Nucleoside-triphosphate diphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Nucleoside-triphosphate pyrophosphatase</fullName>
            <shortName evidence="10">NTPase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">XTP/dITP diphosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">HAM1</name>
        <name evidence="12" type="ORF">AWRI3579_g2796</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) or xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ITP + H2O = IMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>XTP + H2O = XMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28610"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>dITP + H2O = dIMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28342"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61194"/>
            <dbReference type="ChEBI" id="CHEBI:61382"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="10">Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn(2+).</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10 11">Belongs to the HAM1 NTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.66" evidence="10"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB95">
        <property type="interactions" value="738"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB95"/>
    <dbReference type="InParanoid" id="A0A1E5RB95"/>
    <dbReference type="OrthoDB" id="6288734at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035870">
        <property type="term" value="F:dITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036220">
        <property type="term" value="F:ITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0036222">
        <property type="term" value="F:XTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009204">
        <property type="term" value="P:deoxyribonucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00515">
        <property type="entry name" value="HAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.950.10:FF:000009">
        <property type="entry name" value="Inosine triphosphate pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03148">
        <property type="entry name" value="HAM1_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027502">
        <property type="entry name" value="ITPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002637">
        <property type="entry name" value="RdgB/HAM1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00042">
        <property type="entry name" value="RdgB/HAM1 family non-canonical purine NTP pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067:SF9">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE/HAM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01725">
        <property type="entry name" value="Ham1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 10" id="KW-0464">Manganese</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="70"/>
            <end position="71"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="145"/>
            <end position="148"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="173"/>
            <end position="174"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ84169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="188" mass="20887" checksum="5597B3F1A846A56C" modified="2017-01-18" version="1">MAENKIVFVTGNLNKLKEVKMLLQGIKYEIANEPLDLIEVQDSELELIAKAKVAQAIKELGPGTRVFVEDTALSFNAMNGLPGAYVKWFVQKMGLEKMVQLLNAFEDKTGTAVTTIAYSDIQGQIHVFQGCTDGTIVAKRGPETFGWDCIFEPFNTGKTYAEMTKTEKNIISHRGKAFAKFKEFLATX</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBD5</accession>
    <name>A0A1E5RBD5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3043</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBD5">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBD5"/>
    <dbReference type="InParanoid" id="A0A1E5RBD5"/>
    <dbReference type="OrthoDB" id="1666796at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF55">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="377"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="411"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="450"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="494"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="571"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="615"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="649"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="682"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="10">
        <location>
            <position position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ84191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="731" mass="84188" checksum="4A747EF97C22442A" modified="2017-01-18" version="1">MLLSVHKTKVSIAVVFLMAVSFLFFYVDIARLNNPHREEFTYSSNTASKVQEVDQNDNGYFKPNNYFMGKKVPLLFDKIXSTKKPNLKPIPYYDLPFTCYLSEDDDEEEYRPVHANLMDVLTFKRLWASPTNMHFGYNKHCQVICEREIDSNXVDKLTNWIDLEYQYYWYIDXNLAGSTVNAKKNSKQAGFPIGYRDKNRDYYLHTHFSFVIKYENNSFVQQKNDANNNEIITQDLVQENNVIVGFEVEPISLDSYKCPTYSTDYNKFKLVKRDNADVIPYTFSVIWQQVEKTNGKSNYPQRWSNIPGLDESKQFSKGSMELVLCCSLISAYVLFMLSYLYCQSVLENEKICSEIIQAHSSSHSHQGSNFETVFHKISFLFGLQSFALLVAGVIVLSSIFKKHTISYENLWFVAILLACGSATSSLLGTFMSSKNLPTEKTFAKKGNDKFILWASFVYGISLPLVLTVSTKLIICIVELLDQPGVSTLPFVQKFHFRYLLTSFVICFVAGLFCKGTNEKKEISFILKKVKSVAHEHDSVFNQAKFSKSETKCENQEFGLTYASFIVEKLRLFFLWCFSGLIKFLFGLAFFQVLFVGKQTFSSVDQTSYMELVAKMFLMIFFMFIVNSSINITVLLSSLQKSKHQGHWKLLNFFMTYSSFPLALGYAIYYALQIAQVTDLASITFYLFYVGVVMNICFHSALYFLSTLVNLQVLKIIEKSIVEDEYARYEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBN2</accession>
    <name>A0A1E5RBN2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Pre-rRNA-processing protein ESF1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2701</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBN2"/>
    <dbReference type="FunCoup" id="A0A1E5RBN2">
        <property type="interactions" value="1170"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBN2"/>
    <dbReference type="InParanoid" id="A0A1E5RBN2"/>
    <dbReference type="OrthoDB" id="431825at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039754">
        <property type="entry name" value="Esf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012580">
        <property type="entry name" value="NUC153"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056750">
        <property type="entry name" value="RRM_ESF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12202">
        <property type="entry name" value="ESF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12202:SF0">
        <property type="entry name" value="ESF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08159">
        <property type="entry name" value="NUC153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25121">
        <property type="entry name" value="RRM_ESF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ESF1 RRM" evidence="7">
        <location>
            <begin position="171"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="NUC153" evidence="6">
        <location>
            <begin position="587"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="72"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="217"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="418"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="618"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="217"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="440"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="471"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="498"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="618"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ84308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="677" mass="77760" checksum="6CA66DBB7AE7EB93" modified="2017-01-18" version="1">MAASNTGKSGSQTNDPRFANIANDPKFRKAKLNKFKVKLDDRFSKSDLEIGKKKGKKVDKYGRRILDDNELEKKDFDKYFDNDTKDQENEDKKETNASEDNEETSEEETAVKTKPSVSNFDRARGEVPSDYQSSSDESSSDSDSDADSFMEDESDSEIELEEQKPEDTDPTSVVACVNMDWDHLKSADLMMTFNSFVPKGGKILKVAIYPSEFGKERMAREEVEGPPRELFQSKKKRSKKHGKGDNSESDSDSDIEIKDLYEEGDADKDFDSKALRRYQLERLRYYYAIVTCNNVDTAKAIYDNCDKSEYEASSNMFDLRYVPEEMEFDEEPRDSCTELPKNYRPMTFTTDALQHSKVKLTWDETPADRMEVAKRAFSQKEIDDMDFKAYLASDSESEDDEATEEARNKLKSLVSNFGGSSFNNKKPQHDTFGQNRGAELADDGDDDDDEGEVDMEVTFTPALEADDDDEKAGSDKEESTIEKVRRKEQERRKKRKDKIKELKKQAQESKKDKLKSKDVPTTKDAKSTAELELLMMEDGAEPDNKINKKAHFNMNEILKSEKEKGKKSKYQNKERIIEDSFKPNLDDDRFNEIFEDHDFAIDPTQSEYKATEAMKQIMDERSKRKSGQDKKRKANVTNGAKQLNKYKKQKSQDKSSSNTSHSVKELVNKVKSKYKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBN3</accession>
    <name>A0A1E5RBN3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">2-dehydropantoate 2-reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2885</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBN3"/>
    <dbReference type="STRING" id="56408.A0A1E5RBN3"/>
    <dbReference type="InParanoid" id="A0A1E5RBN3"/>
    <dbReference type="OrthoDB" id="3609at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000017">
        <property type="entry name" value="2-dehydropantoate 2-reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051402">
        <property type="entry name" value="KPR-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013332">
        <property type="entry name" value="KPR_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708:SF30">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708">
        <property type="entry name" value="PROBABLE 2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02558">
        <property type="entry name" value="ApbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketopantoate reductase N-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Ketopantoate reductase C-terminal" evidence="2">
        <location>
            <begin position="214"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="40366" checksum="623258A6A385AAD3" modified="2017-01-18" version="1">MTLKVLVIGAGGVGVLTAYSLFHRAKCEVSLVVRSDYEKAVQDGYEIDSCDYGQIHNWKPHHLYRSIEEASASGQFFDYLVVTTKNIPDGPSPVRKFIEPVLKSCFENWDPKTGKSLNVFLVQNGIDIELEIIDLLKADHLCSDGVHGPKVTLLSGVQMIGSTKIGNCQILHKGQDYLIMGAFDPKDTLAISKVAEFKDLYWNEGKNHFELDENVKWSRWRKLVYNATINPLTAIVGLDFTRTLLFGFDGSTEFGILRPAMKEILAIAKSDGHIIPEEVMDYMIDCDRTMVYKPSMMVDVEKTQLMELEVILGNPLKVAAQNKVPCPNLTLLYNIGKIIQGRTKEKIGMITFNEKTGNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBP8</accession>
    <name>A0A1E5RBP8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SWR1-complex protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2590</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SWC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBP8">
        <property type="interactions" value="999"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBP8"/>
    <dbReference type="InParanoid" id="A0A1E5RBP8"/>
    <dbReference type="OrthoDB" id="19740at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032563">
        <property type="entry name" value="DAMP1_SANT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027109">
        <property type="entry name" value="Swc4/Dmap1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12855:SF10">
        <property type="entry name" value="DNA METHYLTRANSFERASE 1-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12855">
        <property type="entry name" value="DNA METHYLTRANSFERASE 1-ASSOCIATED PROTEIN 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16282">
        <property type="entry name" value="SANT_DAMP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="DAMP1 SANT/Myb-like" evidence="10">
        <location>
            <begin position="214"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="74"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="368"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="13"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="78"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ84003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="544" mass="63272" checksum="396865655C8D69AA" modified="2017-01-18" version="1">MASQNDLFDMLNISKQTDSNSTESLVKTSVEAHKKPVQQQQQQQQQQQQQQQPNQQMLTGLQKELYNLMGDSMPPAMLTSNKRQQLKPSPSKASPWMKQKFKPSTDSKIVLKHWVRGIQKDQKDELEISKFESYNHAYTIPXFSEEEFDAFMNTETQQNSNNACGIDEVREKQAQHVATKDANDSEPLSALEKHASPKIISKSKADGTLKYRLTFDEVQYLFDLVKTYDMKWIVIHDRYDFAGKQRTIEDLKDIFYLVCQRYFXNKDPDNIEFQKLLSFPKEKEVQRKAYLSRLLSRTSAEVAEEEALVIEAKKFEMAAKKTSVEREQLLQLLDSPVPLESIDITEFMTSQGLHNFYCSLMGDKTRKRKYDSVPENPWMKQQQRFKTQKTKKFEDESASKKKHQQQQQQXQQNLQKTDKNSDIAAPLFEGTHGRNSVEASQPENIITQLLHEFDQDERKNLGIRLHENKLSPGVFLRSTKISSFKPXTQNKVQAVLNEMKIPLRPVMTTFDVIKKNDELLKKITHLLELKKQVDKLEAELKIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDH0</accession>
    <name>A0A1E5RDH0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent DNA helicase CHL1</fullName>
            <ecNumber evidence="17">5.6.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">ATP-dependent DNA helicase chl1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Chromosome loss protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18 19">DNA 5'-3' helicase CHL1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">AWRI3579_g2441</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">ATP-dependent DNA helicase important for chromosome transmission and normal cell cycle progression in G(2)/M. May have a role in changing DNA topology to allow the loading of proteins involved in maintaining sister chromatid cohesion in the vicinity of the centromeres. Has a specific role in chromosome segregation during meiosis II.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DEAD box helicase family. DEAH subfamily. DDX11/CHL1 sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="17"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDH0">
        <property type="interactions" value="973"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDH0"/>
    <dbReference type="InParanoid" id="A0A1E5RDH0"/>
    <dbReference type="OrthoDB" id="267079at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034085">
        <property type="term" value="P:establishment of sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006554">
        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013020">
        <property type="entry name" value="Rad3/Chl1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00604">
        <property type="entry name" value="rad3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF41">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE DDX11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06733">
        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9 24" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="23">
        <location>
            <begin position="2"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="86"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="22">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00045008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00045702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="OEJ84952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="869" mass="99916" checksum="D7429DE662DF599F" modified="2017-01-18" version="1">MSKSSYNHPYQPYGIQVDLMNAIYTLLDAPQKSSSCSTVNDKSNKARKIGIFESPTGTGKTLSLICSTLTWLRDNKEQLLLKSSKKDXGNSALGTQAGGIIDNNNDYDDDDDDDDDDDDDDDDDDEPDWIVESYNKXINNDKLHDFKEFEKRIQDFNKKNLEAEKLKSTKNNGHLQYKKRKLQRVDVNIETDEDQFLLHEYNDNAMDSRNLLANQVKHLLDKFDQPTTSSKKTAADIANSFKQPKQIIFASRTHSQLQQFASQLTLPNFPSSFEENILSNEKIKYLPLGSRKQLCCNDKIQKLTSIDLINEGCQDAIKTSSCEYYQNQETILQKFPPEAIFQKINDIEEVQSMGKNFQTCPYYASRKATHFAEIISVPYQLLLNNNSRNSINLDLSNAIVIIDEAHNLIDTILNLNRCKITLNELLKIMKSLKVYLHRFNRKLTSTNRLNLMKLLKFLNLIHDYIAKNYKKNIKINPNDILQHGNLDVYNFNELVEFIRVTKLAYKLQSYINNEELKLATKTANVNQTNIETKGGKKIGKGQPLLFKIVSFIECLRNPSYEGIFYFTQEQNTLEYMLLEPSEFFKPIVDNCYKLILCGGTMEPMKEFTEKLFPYVSENEIVKFSCNHVIPDSNLKVFTVTSDLTSHNTQYEFTFAKKNNPEMLESLGSSLLNLFSKIPMGTVVFVQSYDYLQKLVDFWKSNGQFGRLDNVKNIYYETPNSQSDPWKEYSNNILVNRKPSCLFAVVGGKLSEGINFQDDLARAVILLGLPYPNILSSDMVIQRNHIFKKNPAKGKEIFKSYIDMICMRSVNQSVGRAIRHIKDYAAIYLVDKRFANGDVQAGLSQWVKRRITSSEKDIVKETEQFFSLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDY2</accession>
    <name>A0A1E5RDY2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mating factor alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1940</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDY2">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDY2"/>
    <dbReference type="InParanoid" id="A0A1E5RDY2"/>
    <dbReference type="OrthoDB" id="3766782at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000772">
        <property type="term" value="F:mating pheromone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007618">
        <property type="term" value="P:mating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006742">
        <property type="entry name" value="Mating_factor_alpha_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008675">
        <property type="entry name" value="Mating_factor_alpha_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04648">
        <property type="entry name" value="MF_alpha"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05436">
        <property type="entry name" value="MF_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184715" evidence="1">
        <location>
            <begin position="24"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Mating factor alpha precursor N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="135" mass="14366" checksum="EBD85C09717A2E51" modified="2017-01-18" version="1">MKLSTTIAATTAATTALVAGATAAPVSGASNSTSMEVPGEAVKYFLDLSDSPDMALVPINNGNTTGIMFVNTTVIDQAYAEXTSLSRKREASANAWHWLKFDVGEPLYAKRDANADADASXWHWLKFDVGEPLYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE00</accession>
    <name>A0A1E5RE00_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Autophagy-related protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2150</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in cytoplasm to vacuole transport (Cvt), pexophagy, mitophagy and nucleophagy. Recruits mitochondria for their selective degradation via autophagy (mitophagy) during starvation. Works as scaffold proteins that recruit ATG proteins to the pre-autophagosome (PAS), the site of vesicle/autophagosome formation. Required for the Cvt vesicles completion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Preautophagosomal structure membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 6">Vacuole membrane</location>
            <topology evidence="1 6">Peripheral membrane protein</topology>
        </subcellularLocation>
        <text evidence="6">During pexophagy, accumulates in the vacuolar membrane region, where the peroxisomes contact the vacuole.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ATG11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE00">
        <property type="interactions" value="164"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE00"/>
    <dbReference type="InParanoid" id="A0A1E5RE00"/>
    <dbReference type="OrthoDB" id="447953at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903599">
        <property type="term" value="P:positive regulation of autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034517">
        <property type="term" value="P:ribophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040040">
        <property type="entry name" value="ATG11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019460">
        <property type="entry name" value="Atg11_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13222">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13222:SF1">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10377">
        <property type="entry name" value="ATG11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="Autophagy-related protein 11 C-terminal" evidence="8">
        <location>
            <begin position="975"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="276"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="708"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="796"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="9">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1145" mass="131739" checksum="F7FF843D83F3E6FF" modified="2017-01-18" version="1">MAESSTLLINCVKGCSVKVDLSFYADMSSLKSFISMKWXIPVQEILIITPYGAKLKDEDFHNKKSMELLSHLYVFHRKIFSSIKRPVPMSTETTNASIKDDLGKLPFGYSXENTKLSDTQPAQKVDXLYADIDDTQISKLLDQIDPLPTMPKPMASPFQDYASESTSRQAQLKSILQTNLGWLKALKIDVTFYFDQITQFIDSQNHYTQSIEVIVSYLQFYCEQIHSLFLERSXQCETVLXKEYLTNWKSNYARLTQLNCVVPQRNFQHLSEYFDLNELNTQYEEMKRLQVELSXSINELKARLNDSLDLEKIVQSNSKSLEFELIQKFNIDMDLEDNLFNNFHEYIKXIEDITTELLNSDENTLTTKDQTTLLATKNNENLQKLIKNLIPQITIISKSIYTKANDVMLIKRNVQKRLIQFLGEITFVQINALDLKKLLIQNVDKTLHALQLKNXVFEYLERFPVVYGNLLVELIRRKRWLSQIVAVTRGKYNASLVDLQNQELNYRTKWLTNMHSANQYHEYTPVENLGVTKILDLLLNAENKDCEVVIDIINESNFQLNTIKNHYTLTKSSVTNFCKDMIKTGVSEEIVNVLLKNLEKSSHFLLKVETDQKELSSNFQKLDNANDELLTSYKQRVQRLEAQLLVKKLHNTNSWPSGLFQSAATNMDALYQSRLHGKDFGHFSNSNKPINQSSISANIELEKTHTKESQLQEENLALKAANAKLQQELMSLKRENSDLCIENEGYKESLAISKNAIISFTTELETLHNEKIDLSNKFKQDLRILLNQNNKNFNDYDELQNKYEKLNILTAALEKTSETVKTNFDNEKLQLTAEIETLKDQIKTFSXLEQEKAESDKNYNQLQKNFSSYIKELFNIVFNDIFVLENIGLLLERDIYENKETLKITRVKGLRKKLDETTMFLAESQSIDMNSSLVKDLRTLKAKMETGVPENTDEXKREFLQFVQNLYNNESKLYETSVVKRFKDVEQLAKKLTKENKKIKNHFYENGIVVGDLMVGDLALFLPAKIDNDLVNDFSSVNSSMSSIDLGTPPPAPMQTSVSQSVSSVDKPNVXLPAXKNKVWTAFTAFGSARYIVQDLVPIKGEWFIAKISSLEKHTASSFENPYKLDVGVVWYAVSVKPVQRNTTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE79</accession>
    <name>A0A1E5RE79_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1680</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RE79"/>
    <dbReference type="OrthoDB" id="3973181at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="128"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="376"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="432"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="301"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="138"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="164"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="175"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="434"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="451"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="616" mass="70321" checksum="3FADD5597D39C3F5" modified="2017-01-18" version="1">MSYEQENSNEMPMLGIPVQGYMPAAVASPLAPQHQYYYAQPANMGNYCYYPQPMPDYEISYDTHYGLPPMQFPKGPNFNNVNTHSSNNHGKKTPYMGYYGEVEEPKNSNIHYATYKYNMKVRENMNNHYNGTNDKSYKHTNNKNFKNKNHWNKNNTNFDYNRYNGDNQQSSDSQTKSKFENGSTKKKDYSNQTVNNEFLDATLDDLKCSLVQHFEKNRAETGSLNKTFRITTKEASTPYFNKTSNYEFYEFQKAKAQKFLNTLYEIKTPINIDFDQKQDTEISKILTSIGIEQKNASFHSYKNLKAKMTTLEQKILSFEREITNTQNSIENNEISILQSEESTENYKKDKMLLKNFSKKLLCTSSTEEFDEKLKPYDDKLKPYDDKLEPYDDKLEPYENKKQQESQKSANDEGKLVDIXXKPLALQKQYVPVLQPSSPSKSSAGTAPPSPTRNLASSNKYYKTTNNDTGAATVSSTIDAKGAKKYPPKLEQMEETAGIAKIADTDEDAHIPEKDLSIDSIDTSGIRSYKEQPVSNLSANDGFSFVDLSFDGKAMDRSDVFRMCDSFSVDESSFFITQKNISAMANTHGNTASRRGVNNNDQVLKNFLSQEMSSSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REM1</accession>
    <name>A0A1E5REM1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Pirin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1815</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the pirin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REM1"/>
    <dbReference type="InParanoid" id="A0A1E5REM1"/>
    <dbReference type="OrthoDB" id="198735at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02247">
        <property type="entry name" value="cupin_pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02909">
        <property type="entry name" value="cupin_pirin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012093">
        <property type="entry name" value="Pirin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008778">
        <property type="entry name" value="Pirin_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003829">
        <property type="entry name" value="Pirin_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903:SF8">
        <property type="entry name" value="PIRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903">
        <property type="entry name" value="PIRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02678">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05726">
        <property type="entry name" value="Pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="Pirin N-terminal" evidence="4">
        <location>
            <begin position="93"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Pirin C-terminal" evidence="5">
        <location>
            <begin position="253"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ85340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="380" mass="42972" checksum="6957937F84F0E5A2" modified="2017-01-18" version="1">MEQKSVETGKHNLDRRHPFSFVLSSSFISFVIYLLSGILITYIAFHFQETKNQNKQQLQQVKQIDQLQPQQTSMTRSVLKSFLAIEQSEGVGAKVRRSIGTGQMRNFLPFLMLDHFNVQPPAGFPDHPHHGQETITYITEGMMAHEDFTGSKGVLRPGDLQFMTAGKGIVHSEIPVDSGDGKSAIGLQLWVDLPENLKNCEPRYRDLRKEEIPEANPSDFLKIKVISGKSYGVESLQDLAYTPVHFYHFQTSKKGTVFEQYMPTDFNSFIYILKGSITINDKLYPQYSSIFFNTDSDIVKGASASEITEFAIIGGQILDQPVVQYGPFVETSKSKILEVIQNYQQGKNGFEKAPHWRSSISDGIEKKRANEFLEKDGLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REP0</accession>
    <name>A0A1E5REP0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Flavin carrier protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1875</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transient receptor potential (TRP) ion channel family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REP0"/>
    <dbReference type="FunCoup" id="A0A1E5REP0">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REP0"/>
    <dbReference type="InParanoid" id="A0A1E5REP0"/>
    <dbReference type="OrthoDB" id="5212126at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010308">
        <property type="entry name" value="TRP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040241">
        <property type="entry name" value="TRP_Flc/Pkd2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032800">
        <property type="entry name" value="TRP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145:SF4">
        <property type="entry name" value="FLAVIN CARRIER PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_7G01610)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06011">
        <property type="entry name" value="TRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14558">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01320">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="338"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="386"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="412"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="474"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="500"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="531"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="562"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="ML-like" evidence="9">
        <location>
            <begin position="31"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="675"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="721"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="773"/>
            <end position="802"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="811" mass="90887" checksum="6FCF6A193816A119" modified="2017-01-18" version="1">MIFIRSLQNTFITYILASLFLVPATLVQARRTLSASSLVSCMQNSQLVAKSFDVYFDPDSSALHYSLDINTEISGEITAYVDVYAYGFKIISRTIDVCDIGWKQFCPISPGTINVESIQYISEEYTKLIPGIAYQVPDIDAVARLKIVKSDDLDGQVLACIQAYFTNGKTVAQTGVKWATAVVAGIGLLISALLAAFGNSVAATHIGANAMSLFLYFQNVAIISMLHVEYLPPIASSWAENLVWSMGLIRISFMQKIFRWYVEATGGTPALFLTSTTYSVLPQKLRKRALDYIKPLVKRAKQPVLYGNSNVKIFRGMKRVGYRMNIENTSIVPTGFTFFILCGYLLAAIIIASKFLIELFIKWGWVKNSRLISFRKSWTAVIKGALLKYFCIGFTQVAILGMWEFFERDSPAVIVLAVIYIIVILGLMIWSAYRTWYFGRQSTLAYQNPAALLYGDDRVLTKFGWFYSMFNAQYYWWGGIVLLYSFVKALFVALAQNSGMTQALAIFIVELIYFGALIYFEPYLDKRTNVLNILIQTVNLINSFLFMFFSDLFGQPAAVASIMAWVFFILNAAFSLILLLMVLVYTGIVLWKYKNPDVNFKPARDDRFSFQKGDSYVGALDDKVAKELLSLGTAVKQHDTNWEDQLQEQKQVMQNEEYNASTQMFQTKANGSYADLLGESSSSTDTNEKVENSASSATNEAHPGLKSRLKSFTAKLKRNPTMDSTASFTSLSKRPTNDPTPEMYISPSQIPSDKSHERTASESKTGLMKSAHSADNLTDSLKYPQTLNREASSDSFYNQEAQGTADVVNRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REP2</accession>
    <name>A0A1E5REP2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cardiolipin-specific deacylase 1, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2116</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REP2">
        <property type="interactions" value="154"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REP2"/>
    <dbReference type="InParanoid" id="A0A1E5REP2"/>
    <dbReference type="OrthoDB" id="7457040at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042171">
        <property type="term" value="F:lysophosphatidic acid acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035965">
        <property type="term" value="P:cardiolipin acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886:SF29">
        <property type="entry name" value="PUMMELIG, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886">
        <property type="entry name" value="RE40534P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="143"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="439" mass="50279" checksum="C56C3DEFDB67FBB8" modified="2017-01-18" version="1">MPKLLYGSFYSAVKTLANHLPLVKTSKTIASATNSALKQSGCAETQYRLSTPPSAIPLLKIIKALPTMFPLSWEXSLRDYEQFNRDAKKFQTELFSTLPFYPNPANRITSEFIETHVGGDAYINEFCVRPTFAKKTTXGKPQKHLILVHGYGAGMGFFIKNIEHLARNLIQDNWCIHAIDLPGYGYSSRDKFPFKIHSDTPEXVEEWFHAKIHQWFRARGLLKNPDQNTVLAHSMGAYLMATYAAQPSLQNHWSKLIMCSPGGITPTKLQPNPPLWFVKLWDQNISPFSIVRTLKRFGSKITSAWTFRRFSNLGNSRLKLLLHRYTYAIFNAKGSGEYMLSFFLKCGGDPRFPLQKRFFTNADNNNIHIPNILRNTKMEWIWLYGQNDWMNVDGGIACSKFLNAHDIKSKVAEIPNAGHHLYLDNEQIFNEFVLKELKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REW0</accession>
    <name>A0A1E5REW0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">L33</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">YL33</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2381</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REW0"/>
    <dbReference type="FunCoup" id="A0A1E5REW0">
        <property type="interactions" value="917"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REW0"/>
    <dbReference type="InParanoid" id="A0A1E5REW0"/>
    <dbReference type="OrthoDB" id="1688503at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000009">
        <property type="entry name" value="60S ribosomal protein L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005825">
        <property type="entry name" value="Ribosomal_uL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005756">
        <property type="entry name" value="Ribosomal_uL24_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="Ribosomal_uL24_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01080">
        <property type="entry name" value="rplX_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11143">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L26 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16906">
        <property type="entry name" value="Ribosomal_L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01108">
        <property type="entry name" value="RIBOSOMAL_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="KOW" evidence="7">
        <location>
            <begin position="79"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00080137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00082602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ85440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="152" mass="16790" checksum="255BEE513DB234FC" modified="2017-01-18" version="1">MAKQSLGMSNTTINANSGFQRPEVSSNNRAKNVSSDRRKARKAYFTAPSSERRSLLSAPLSKELRAQYGIKALPIRKEDEVLVVRGSEKGKEGKISSVYRLKFAVQVDKITKEKSNGASIPINVHPSKVVITKLHLDKDRKALITRKGGKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF28</accession>
    <name>A0A1E5RF28_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADH:ubiquinone reductase (non-electrogenic)</fullName>
            <ecNumber evidence="3">1.6.5.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g1683</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a quinone + NADH + H(+) = a quinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:46160"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a ubiquinone + NADH + H(+) = a ubiquinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:23152"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.9" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF28"/>
    <dbReference type="FunCoup" id="A0A1E5RF28">
        <property type="interactions" value="156"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF28"/>
    <dbReference type="InParanoid" id="A0A1E5RF28"/>
    <dbReference type="OrthoDB" id="3244603at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) (non-electrogenic) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015980">
        <property type="term" value="P:energy derivation by oxidation of organic compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.100:FF:000007">
        <property type="entry name" value="Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045024">
        <property type="entry name" value="NDH-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054585">
        <property type="entry name" value="NDH2-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706:SF10">
        <property type="entry name" value="ROTENONE-INSENSITIVE NADH-UBIQUINONE OXIDOREDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22366">
        <property type="entry name" value="NDH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="15" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="13">
        <location>
            <begin position="81"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="External alternative NADH-ubiquinone oxidoreductase-like C-terminal" evidence="14">
        <location>
            <begin position="472"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="39"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF22366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ85173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="542" mass="59711" checksum="AF4FEB7DC98A461E" modified="2017-01-18" version="1">MLSSTRNATNVQMSVLLKKQHARVGYMFYNKNNCRSVSTTSAKAAPSAPKEGTVGTTGGGVSSFTTTPKIINHSQSSNKPNVVVLGSGWGAISFLKNIDESKYNVTVVSPRNYFLFTPLLPSTPVGTVDEKSIIEPVVNFALKKKKGNVSYYEATATSINPDRNTVTIQSVSNVSQTKGDKPNLGIQQNDTCELKYDYLVSAVGAEPNTFGIPGVEKYGMFLKEIEHSLKIRKQFIANVEKANMLPKGDPERKRLLTIVVVGGGPTGVETAGELQDYVDQDLKRFMPSIAEEVQIHLVEALPVILNMFEKKLTSYAQSVLEQTSIKLELKTAVSKVEEKYLIAKTKKDDGSFTETTIPYGTLIWATGNKARKIVTDLFPKIPEQNNARRGLEVQENLLVNGSKNIFAVGDNAVAGFAPTAQVAHQEAEYLCKVFSKMSNNPSVYDELANAKDAKAIQTVFAKNGIKPFKYVHYGALAYLGAEKAIANITYGKRSFYTGGGSLTFYIWRVLYLSMILSARSRFKVIADWFKLALFKRDCFKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF73</accession>
    <name>A0A1E5RF73_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2270</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RF73"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ85562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="224" mass="25753" checksum="DC6D2F471F0BB087" modified="2017-01-18" version="1">MLEDNRYSACHIEKLDNTKKRYEPLRAMIEYIPVVDDPSSNIDTRSSGFAGYKRKTEXMIDILATQSRTQTFEQDNSNLHPHVREKYPLPISYFKHLHEANKSKRPVSETDSSLTLPGIPSKKLKVERSSLEXQKIKYAYLKHQQLHLKGALDNHIVSKQCVVLNRCLNSSAGDLQSRLEQSLKNSESLDRYNAAEINKNIPLIESLNNKWKEQLIQNINANSX</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF91</accession>
    <name>A0A1E5RF91_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">Ribonuclease P/MRP protein subunit POP5</fullName>
            <ecNumber evidence="4 10">3.1.26.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2220</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP, which cleaves pre-rRNA sequences.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the eukaryotic/archaeal RNase P protein component 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="4 10"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF91"/>
    <dbReference type="FunCoup" id="A0A1E5RF91">
        <property type="interactions" value="286"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF91"/>
    <dbReference type="InParanoid" id="A0A1E5RF91"/>
    <dbReference type="OrthoDB" id="24745at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030681">
        <property type="term" value="C:multimeric ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3250:FF:000004">
        <property type="entry name" value="Ribonuclease P/MRP protein subunit POP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3250">
        <property type="entry name" value="Ribonuclease P, Pop5 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002759">
        <property type="entry name" value="Pop5/Rpp14/Rnp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016819">
        <property type="entry name" value="RNase_P/MRP_POP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038085">
        <property type="entry name" value="Rnp2-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15441">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15441:SF2">
        <property type="entry name" value="RIBONUCLEASE P_MRP PROTEIN SUBUNIT POP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01900">
        <property type="entry name" value="RNase_P_Rpp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF023803">
        <property type="entry name" value="Ribonuclease_P_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160350">
        <property type="entry name" value="Rnp2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR023803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="168" mass="19400" checksum="8773E173787E2A0B" modified="2017-01-18" version="1">MVRFKSRYMLFEILYPPTAEEYTAFTEANNFVLEPALLQHHKVTPSDVNNRVLVYEIKKSLQHFFGDYGLGRGTSMFQLKYFSNSTSTGILRCSKDDYEYVLMTLNMMKKIGTLENVIINVVKVSGTIKKLEEFAISRSDELMRALNQDKKDFLQSMSSIPVDKNEDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFE6</accession>
    <name>A0A1E5RFE6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">DASH complex subunit DUO1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Outer kinetochore protein DUO1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AWRI3579_g1776</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex DUO1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFE6">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFE6"/>
    <dbReference type="InParanoid" id="A0A1E5RFE6"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013960">
        <property type="entry name" value="DASH_Duo1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28216">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DUO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28216:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DUO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08651">
        <property type="entry name" value="DASH_Duo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="16" id="KW-0137">Centromere</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="130"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="194"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="235"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="194"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="246"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OEJ85628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="270" mass="30086" checksum="8F7EC9A68D185CA1" modified="2017-01-18" version="1">MSQKDFTAPMEIDDETISKIVPTLFTEMNNKFSRTRPSFTNTNTFQENTSTTEDIKKDENTSIQALLQESEQLDAIIPVIGNISSTMDEKISQYLLQTKQTCTSTIKILDTWNNIFSQAGYMKTLSEYANKNSGRSDTVXREDQKTGSRPPKSNAEILHEKMGELEMAKKTLKDLQAAKANPRARAALNRRTPTPGYAAATTASARGKIQKAPIPSTSGSRVKDLKVANMGNGILKKNDKAVNPYRVQTKSTQSRPYVGQSKPRRSLFDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFH7</accession>
    <name>A0A1E5RFH7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ribosome assembly 1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1917</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFH7"/>
    <dbReference type="InParanoid" id="A0A1E5RFH7"/>
    <dbReference type="OrthoDB" id="273070at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019496">
        <property type="entry name" value="NUFIP1_cons_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10453">
        <property type="entry name" value="NUFIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMR1-interacting protein 1 conserved" evidence="2">
        <location>
            <begin position="343"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="493" mass="56476" checksum="B0E0CB5125E4415F" modified="2017-01-18" version="1">MNNNNNNNMNSYGYSNGNNYNXSQQYNQNNNNNNNQNQQQMNYGNNXGYQNQQQMNYGNNNNYQNQQQMNYGTGYPYSSGPQPQSNAVSYGTPTYYPPNQNPYIQGTPATMPSYGTSSVSGQYSFNGYNSYMNTTSNAYQQMVIQPTSSGKSLRDYQDSDDDFNGDGLEESDGDDXLNGNNSKGYHQKKXGKQYKKKQYKLPLPSNAKDGSKEKKEVQEFDYDEDVRNMXILKKRKKQMQIEKEQEIELKDHVKTEGKISEGDGKHDTLQKDKDDEEKIDNNQVLDQTMKNLSEISKASVAQTLATFEDSENDLTQTNKKRKLSAEFSDEDXNTKESTTNKPNSVVTIPGTSISLQTEEEIQAWRNERKKNWLIKISNKKEEHCENLGIDASELAHSTNRKKFQEFKKDKDFIDRISMQIKKDAGFENQLTKPSSLTTTMLKRDQNEENSKLLQFIKELGDAQYLEYELTEQEKSKLFKNSNNNFSGGKQLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFL6</accession>
    <name>A0A1E5RFL6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">tRNA pseudouridine(38/39) synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2325</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFL6"/>
    <dbReference type="FunCoup" id="A0A1E5RFL6">
        <property type="interactions" value="731"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFL6"/>
    <dbReference type="InParanoid" id="A0A1E5RFL6"/>
    <dbReference type="OrthoDB" id="25767at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02569">
        <property type="entry name" value="PseudoU_synth_ScPus3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.580:FF:000020">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041707">
        <property type="entry name" value="Pus3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00071">
        <property type="entry name" value="hisT_truA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF5">
        <property type="entry name" value="TRNA PSEUDOURIDINE(38_39) SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase I TruA alpha/beta" evidence="5">
        <location>
            <begin position="249"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="63"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="63"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ85667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="494" mass="56921" checksum="3C09C49FF0CBFCB8" modified="2017-01-18" version="1">MSKQDGLVRRILNRIYKPNTSAIASGEVSGKLGDSQVLAKYNKWSREQLILKIQELEDAKLQQHLTPTHTRNGSTARSTKSEKHNSKKPAKPFDYSKYNTRFIALKFAYLGWNYNGLAIQQTPTPLPTVEGVILDVMLKCKLIPNMNIYEYNFARCGRTDKGVSAMNQVISLNVRSNLTPDEQSDPANDSKEIDYIRVLNNKLPPDIRIHSVMLRPPPGFDARFSCKYRHYKYIFKKTNGLDVETMKKAAALYVGPDKDFRNFCKLDGSKQITNFKRHVLSAEIVTLNETEQLYCFDLKGSAFLWHQVRNMMAILFLIGQGLEKPEIITDLMDVEKYPRKPIYDMANDIPLILYDCKFKQELDAEKFDVDTENKEILKDEQYGEQRNVCGLEWISGSKFTQHGRNLNNVFATILDYEIKNEVSKIFSHFSKDHSTVMAMSETNLTDKNKVCIDTGDGRYKNTGKYEPISKRHVQSLYKDINAKYKQKKARKLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFM1</accession>
    <name>A0A1E5RFM1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1767</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFM1">
        <property type="interactions" value="243"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFM1"/>
    <dbReference type="InParanoid" id="A0A1E5RFM1"/>
    <dbReference type="OrthoDB" id="10256176at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="316"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="469"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="316"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="810" mass="93494" checksum="F0222A2487A30258" modified="2017-01-18" version="1">MSPTRNMLMSEIYRKXNNSNLYYEGLEDIEELESPTDQELQEEQDEGXFSPTEDEMLHELYSNFTKRSRVGLPYMETTESFKSIIPDPNAPEDFKYSDENIQDCIEKLSFLPWNNLKDFSSTVTDDYDFLGIVVDRLNAIKFVXPFNTDSNEQAYNSRIISLNLAYHYKYHVSHYRAFRSAHERMQSYVDFLKLLNVQKDEKMMAMMDKAAIGESALRNSLSLIRTETAFAKYKYSWELLQLWMTQLFEVTCGASXLIDQRSLQDIKSKLSTSTEGEADNFKSNANKDTNYGANDINXFDXDTGLKNDFSLKFNEKTSASXDKPQTGSKDTGETNLYDDQDDQSSRSSDPKHCSRESPLPPSLLLSNDNPRCNSAQKVEFLMVRPSASDMFGLRLALDEPNIKITDYELVMNNNQNIFSPKFFEAKDTLTPKSMSKIDYDNYILNMMDLPKNKHSQLLQKDLSALLPAHNSLQVDLKRLQDELFQLKLRIDEHHKKSQLIHTSHSVGNGMKGSIDDQALKHDLETQVLLKQKIIKVIKTINKTSLKINSVLLAQQYSTSYSNYTKIKLSPEIANFLYKQEMKEKYKQILIQEQQFLVKERAPEHDDFQKTNKLDAAKLRENXTKSPLITKIPFLEDSFHCVSIPGIWITIPYKKWEPLAKEVYRVLKPNGVLYTPSVDFQPVNMHDSASSNFKTSREKQAIFDNIVLQSTKSGHQLAPTRYLTQVLYKAGFKTVKYTMLSIKLGDFQNDMGKNNELIFSILFMLFFMNMRQNANLPDVDTFIERYMKEHENLVDPNAGSIRVVLIKAYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFQ2</accession>
    <name>A0A1E5RFQ2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Vacuolar cation-chloride cotransporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2025</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC12A transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFQ2">
        <property type="interactions" value="187"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFQ2"/>
    <dbReference type="InParanoid" id="A0A1E5RFQ2"/>
    <dbReference type="OrthoDB" id="2020542at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015379">
        <property type="term" value="F:potassium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006884">
        <property type="term" value="P:cell volume homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055064">
        <property type="term" value="P:chloride ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055075">
        <property type="term" value="P:potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034486">
        <property type="term" value="P:vacuolar transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000013">
        <property type="entry name" value="Solute carrier family 12 member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018491">
        <property type="entry name" value="SLC12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004842">
        <property type="entry name" value="SLC12A_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827:SF72">
        <property type="entry name" value="GH08340P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03522">
        <property type="entry name" value="SLC12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="200"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="363"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="432"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="465"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="9">
        <location>
            <begin position="77"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="10">
        <location>
            <begin position="557"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="906"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="913"/>
            <end position="923"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1078" mass="119948" checksum="9782364004EAF247" modified="2017-01-18" version="1">MESTFYQLPGSHKPSNSSEATCSTPLLAKKGVQMQNKGSFSYFTGNAAGNDVMQRKISSKFDPENPHKHKLGTYDGVFVPTTLNVLSILMFLRFGFIIGQMGILGTLALLVVSYLIDLLTTMSVSAIATNGTVLGGGAYTLISRCLGPEFGGSIGIIFFIGQILNSGMNVVGIIEPIMFNFNAQSGVIAPILPTGHWHEFMYSTILLMICFGVCLIGSQTVSKVGNVLFWLLLMATVSIPISALCMPAFSSPIEYTGFSIKTLKENMLPHFTSGAAGSLLKKKETLNDLFGIFFPATAGIFAGAGMSSELRKPSRSIPKGTLWGLLLTFSCYFXVIVSLGSTVPRSSMHKDVSIIQTVSGCQPLILIGEMATSLFSVIVGIIGAAYVLEAIAKDEILPGLNCFHKHPSLALFLSWFLTQLCLFSDVNQIATFITMTFLMTFIVTNVACFLLEISSAPNFRPSFKYFNRFTASMGGLLSIVAMFVVDGFSALGVMFSLIALCLLIHYTCPPKQWGDVSQNLIYHQVRKYLLRLKQDNIKYWRPQILLLVDSPRSSWNLIKFCNHMKKGGLYILGHVITVKKAHEFQKNYNELQLQKTSWEKVRDYLKIKAFVHIGLGQSLQWGVRNVFLGSGLGGMRPNITVLGLIESEKKSEKYLKNGEIPAFLPTDNCKNESRITKQQWVQLVEDLSLMKSNIAIAKGFYDLELPLKNHTPKQKKYIDLYPIQMTANLTNGKVSTKTTNFDTYTLILQLGAILTTVPAWHKSHILRCIVFVENEFEKIDEYKRITQLLSVLRIEARVHVLVLSQFRVYNTIIKGDSIALKYVNQQLKNDAWWADIVKARKDTNFSSRRFSYGDYNNKYRIDKKLKPKSTVSELQRFGVSLTMSSNIPVANDMFLFNENAGALDESADETDLESSTPSVSTTQKKPKNTKKEDKSNIIPVFSSDAIPRTKVIEDATGVEPSLIPVENAYVPIKKHIKPQLSPCQSMDNLHLKELDFNSVPRKAQLLILNDMMTQLSGNNSKESCNTNLILSTLPIPMLGTHMDEEASEDYINDLNLWLDELPPTLLINSQSMTVTTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFS6</accession>
    <name>A0A1E5RFS6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial outer membrane protein OM14 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1762</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFS6"/>
    <dbReference type="InParanoid" id="A0A1E5RFS6"/>
    <dbReference type="OrthoDB" id="3972918at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990593">
        <property type="term" value="F:nascent polypeptide-associated complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006626">
        <property type="term" value="P:protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039454">
        <property type="entry name" value="OM14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039453">
        <property type="entry name" value="OM14_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38402">
        <property type="entry name" value="MITOCHONDRIAL OUTER MEMBRANE PROTEIN OM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38402:SF1">
        <property type="entry name" value="MITOCHONDRIAL OUTER MEMBRANE PROTEIN OM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17304">
        <property type="entry name" value="OM14_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="209"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Mitochondrial outer membrane protein OM14 C-terminal" evidence="3">
        <location>
            <begin position="169"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="235" mass="26688" checksum="89BC0503A457C798" modified="2017-01-18" version="1">MAQSPNNTVPGSIPADPKHPQETSINPPEVQEEIKEHLQEQHEHLKEKREHQHEKEKLKEVEAKAEKEKAHLAKEAKHKKDEFVSSAKHKKDQVASKAKKEAGKLEDEVESFSKKFSNEASKKYHQSESIFKQYLQQSVQLVRNLPAYLYEGGQKTSNAMVVYTKSFVQELKNPVFATHFVGGVYLVSKLLNGYAHHARYLKGKSDKDILMIAGGVGVLGIADVWVSKKLYKKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGH2</accession>
    <name>A0A1E5RGH2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Chromatin structure-remodeling complex subunit RSC4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1374</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGH2"/>
    <dbReference type="InParanoid" id="A0A1E5RGH2"/>
    <dbReference type="OrthoDB" id="3971902at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04369">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037382">
        <property type="entry name" value="Rsc/polybromo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16062:SF13">
        <property type="entry name" value="CHROMATIN STRUCTURE-REMODELING COMPLEX SUBUNIT RSC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16062">
        <property type="entry name" value="SWI/SNF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="10">
        <location>
            <begin position="118"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="10">
        <location>
            <begin position="264"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="725"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="729"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="750"/>
            <end position="759"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ86002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="834" mass="93422" checksum="EE4D39DD423E1AC7" modified="2017-01-18" version="1">MPPKKRNFTQVAGSGNPSNEHPTQPAHGYQESTQDHGLQMNDSSATGLIDPETGKHMYXPGKRPRLFSDVLEVDPSKPMDXREELWIDRANWDIPNLNSSVEEVIDTFIRDTDSDNYQKTGFFRDFIKLPSRKFYANYFQKIEKPISMKEIKNRDYQVINSTPSNYEKLLLDLELLYKNCRAYNHEDTLIVKSGQQCVYYLKHEFSKLKNVKRNYLLNGGVLELITERIIKPIEISTEVTISEKLKKVYDDEDWSTLEGRPLDDETSMIEPFLELVDKDEYPDYYQAIYTPLSFQMIKGNLAQGFYKTVYDFFIDLQLLFQNCLVFNHEETEIHKDGQKLLDYTKVLFYELMDYLHTRPSGIVLDIDKLAYEKYLFNSSKAAASSSKVITSFAGSINSSVGGANMNKKGSPNATTXNFTGDNDFNASVNGAGSIIKTSLTNVSSTNSNSLANSGVVTTNPGVSAMGNVSVTNYSGNGIFPTRNTNKLFSLEAFSSSNPMSIRDQAMLSNDEFRNVEGSATFEFDYNQEHLGAKNSYPFGSTFSEVIDKSKFIWKNILQKQDSGANTSLGHNEHVGVNFIDYIKISTSXDSNAAPSALNSFGQNKKDQMLSFNEIIIKNDQQTKNGLNSPHRSKNLSIPARKQIINIEVNFLLSLNKKQTDETEQRGNGNEADAAYQLTGGKEAADEKESLYKTYKTSINLNGTSIGNAMESAPFEFFETVLEDAATNKTSEPSSGTXTEENTQGKEQNEVXKSTENGMQTLEEKDISNGEALNRETTTALPIKSTVPKYKLNPLSYNFKLPEGLHKLNISVSSSTPSDSSSTDTDSICLWLNVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGJ3</accession>
    <name>A0A1E5RGJ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphatidate cytidylyltransferase, mitochondrial</fullName>
            <ecNumber evidence="6">2.7.7.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">CDP-diacylglycerol synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AWRI3579_g1578</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TAM41 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.41" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGJ3"/>
    <dbReference type="FunCoup" id="A0A1E5RGJ3">
        <property type="interactions" value="360"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGJ3"/>
    <dbReference type="InParanoid" id="A0A1E5RGJ3"/>
    <dbReference type="OrthoDB" id="341477at2759"/>
    <dbReference type="UniPathway" id="UPA00557">
        <property type="reaction ID" value="UER00614"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004605">
        <property type="term" value="F:phosphatidate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016024">
        <property type="term" value="P:CDP-diacylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015222">
        <property type="entry name" value="Tam41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13619">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13619:SF0">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09139">
        <property type="entry name" value="Tam41_Mmp37"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028840">
        <property type="entry name" value="Mmp37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10 20" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="17" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 20" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00029893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OEJ86022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="402" mass="46129" checksum="EA9B568BFE36EE37" modified="2017-01-18" version="1">MLHKKSVLNQASITSLRIIRNQCSAKALRPAFCTKHGDTSKHGHTNHHEEHILSNHDGGNLVLKNTGFHFESTKSNDTGKLDLLEKELQKVDEFNKNFNNYNYEFTRLPPNFADNQFVPINKGLQDELQNILGCFDQSPIKYAFGYGSGVFEQKGYSNSKEEKPQIDMIFGVMHPNHWHALNMRANPNHYSSLKYFGSEFVAKFQEIGAGIYFNPFADINGHQVKYGVVSMERLLKDLATWDSFYLAGRLQKPVKVLKNDLRVQYWNQLNLKAAATISKHITLTKNNGVFDEFKFYENLTGLSYLGDIRYKIGAENPKKIQNIVSKNLENFRYYYKPVLKDVFENTSTHLPKGYTLENYAQKLESTIAINSLQQTLKGIFTAGIAKSLKYAWLKKMKAMKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGJ4</accession>
    <name>A0A1E5RGJ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">GTP-binding protein YPT6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1843</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endomembrane system</location>
            <topology evidence="6">Lipid-anchor</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGJ4"/>
    <dbReference type="FunCoup" id="A0A1E5RGJ4">
        <property type="interactions" value="501"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGJ4"/>
    <dbReference type="InParanoid" id="A0A1E5RGJ4"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01861">
        <property type="entry name" value="Rab6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000970">
        <property type="entry name" value="Ras-related protein Rab-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015157">
        <property type="entry name" value="TMA7"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09072">
        <property type="entry name" value="TMA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="268"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="295"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ85693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="304" mass="34033" checksum="F6FD6625163D862B" modified="2017-01-18" version="1">MSGRQGGKLKPLKQKKKQNNDYDEEETAHKEKLRQEQAAKKAMMQNIKAGKPLGGGGIKKSGIHQEPTKKQLLEQHAQPFNILFMSGLQVEGAGNSVTKYKIVFLGEQSVGKTSLITRFMYGTFDDHYQATIGIDFLSKTVYLEDRTIRLQLWDTAGQERFRSLIPSYIRDSHVAIVVYDITSKKSFEYIDKWCEDVKSEREDDVILCIVGNKSDLSDERQVPIEEGERKSKILGASIFIETSSKDGYNVKTLFKQIAKSLPDFQSSNKNKDDLINENSAGGSKQGVIDLSSANGDEDSSSCQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGS2</accession>
    <name>A0A1E5RGS2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Restriction of telomere capping protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1559</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in a process influencing telomere capping.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RTC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGS2"/>
    <dbReference type="STRING" id="56408.A0A1E5RGS2"/>
    <dbReference type="InParanoid" id="A0A1E5RGS2"/>
    <dbReference type="OrthoDB" id="128308at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039024">
        <property type="entry name" value="RTC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028094">
        <property type="entry name" value="RTC4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41391">
        <property type="entry name" value="RESTRICTION OF TELOMERE CAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41391:SF1">
        <property type="entry name" value="RESTRICTION OF TELOMERE CAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14474">
        <property type="entry name" value="RTC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01312">
        <property type="entry name" value="RTC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Restriction of telomere capping protein 4 C-terminal" evidence="9">
        <location>
            <begin position="301"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="151"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="62"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="106"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="458" mass="53002" checksum="9DD117799CF6104C" modified="2017-01-18" version="1">MGRSAADTSFDRDTPSKQNGQRKLYTKNVLTTLSDSGLSAMKTPISSLRSKLRNNLAAQTTPKKRKLSKHESKKRHDASALSSCSDDNNYDGDDEKKDGNFEDNDTQGIESASSSDIENDLELAKTSLVKRHKYQNEPEFKRYSIRNSTKRIDYKETSSENSSTSGSEGFEQDDAGEDHENKNDSNDIDIDIDIGSSTRRNNSFTFYGMEDMPVWDAKYTDLEHYKKDMDDDLVNAAQIDPDSLKKDYVKLNTYDDKMMLLNKKLEVRTKYTTKYPTLPVPPYFLELKDQIEPYLKEIPDLLKAKTTTFYYATAKEMHMNSSRPAVTDGDIKAKLMSEPEKLSAGAYGLKRQFQVGEWIIDRYQKEIMERYGNPVIKWWGYEDFARYILSCDVLTRLLKDQMRLRSLKKAFEVMNDTVEYGNFVSDDDPFHPWELDIELEKIKESNLDEKYQSTFYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH56</accession>
    <name>A0A1E5RH56_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleolar protein 19</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH56">
        <property type="interactions" value="127"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH56"/>
    <dbReference type="InParanoid" id="A0A1E5RH56"/>
    <dbReference type="OrthoDB" id="4068385at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022592">
        <property type="entry name" value="Nucleolar_19"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10863">
        <property type="entry name" value="NOP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="212" mass="23626" checksum="002CE10B0982DB0D" modified="2017-01-18" version="1">MSRAKEIKEKQELQAKLASAFSSKTATVLSWLSTDSVNGANKISKDTALLSDARKDFFNLPVVATGAGLSFEDEQFTAKKNETNHEDIATVGEFIKSNKKXSTLAKKKMQNSNPRDXLNSTVYRIRKDDSKATIALKNKLRNTKRNDLRNQMQNNPSGKAFXNGRTNGGNIDPSRQRPAEESEDDGEEHEQIIQKTTKKSGAFPLQFGKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHK1</accession>
    <name>A0A1E5RHK1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Topoisomerase 1-associated factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1585</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the timeless family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHK1">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHK1"/>
    <dbReference type="InParanoid" id="A0A1E5RHK1"/>
    <dbReference type="OrthoDB" id="310853at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031298">
        <property type="term" value="C:replication fork protection complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043111">
        <property type="term" value="P:replication fork arrest"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044998">
        <property type="entry name" value="Timeless"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006906">
        <property type="entry name" value="Timeless_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22940:SF4">
        <property type="entry name" value="PROTEIN TIMELESS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22940">
        <property type="entry name" value="TIMEOUT/TIMELESS-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04821">
        <property type="entry name" value="TIMELESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0236">DNA replication inhibitor</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0469">Meiosis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Timeless N-terminal" evidence="11">
        <location>
            <begin position="74"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="343"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1023"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1083"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1233"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="345"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="362"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="387"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1028"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1084"/>
            <end position="1097"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ86399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1306" mass="149341" checksum="44E3A7DBCECFFADC" modified="2017-01-18" version="1">MEVEDSMFLNDQDEVEIVSPNEPAPIQSEHTKKSQFPTSILHARIALLATAIGGPEYSIEHTTDSNVHAQVPVHYKHGDDCLACLKDLKKWFKLVDDNQRKYEVAVACANYKILVEDLIPIMLDYETRCKNKKKRERRKKKIGELEPMSLFPVSSPYFDKIALQSLQLMVLMTWPVVLNNDSSANQILHYNTLKKHQLLYKKKILESNVLKPVLRIAVAKISTNKQERSPKDHQILKLVLQFLRNVLAIEPFQMVLTTKKLAQLRTKTKSFHQSSPLPNNVTLEDISLSTTLRNFKTCKVFPVLLTLANSLNTEFDPDFLNQTLAEIFFYLIKSVPPNALLTPMSHENGTPNGISNGISNGTSNRISNATSNATSNATSNGISNGNPSTTTNTHHANSDLTSLLSKEKQLRKNVVRNTSSRHSRFGSLISIQTPDNGRLTVSGGTAKDLIDKNTILNKLDDRKKWNKSIVFKDTETVDGLQSNFLQFTKTNNSSNSLNTDVARFLQQFLQDFLDSNSFNILLKSCTDYLISEDFLSEIIQIQYLIFYSWFIKYQTCRILDSSANSPKDKEARIGTIEYVMQDTSIVFTTKLLKEAMENKQWAVVHANIVAYTELLTFLNNLDDFYPQDCKYMKEKLFSDDRINLLTMLPRLANKHSVSFVRSTIDLTHMVLKTLEKDSELEIKSHKLKSQKTGSAKMHGNLKVKLEEIKKTENISDDDEAMEVLEDQMMSKKIDFENIMQKYFHNDTVKTYISFLKKYRDLEYHDMKRTLQFFQRLFVEHKEESYLFRLDFIVLLKEMLSPDGLPRSDKIRKHLTTFTNYYLHKLKERLKKSPTWYVDLLFPNLTDKAVGFYSKYGQVNTNFLQNYLCCLPSEFKKLPEEEALPPTVVLDMKFGILVSTLIDDNKYDILTDLLAHLKHSLETLSQDQSKNIDLPHNDNSMDSEMHRLCRGDADFRALLLLLGYTVPVKIADLCYLPKATECSKIRAGISMVSKYMNTSFDTPNGTSSSSYLIRYADDGMVTKKSASKARHEQTSENYHSNDENDYDNDVFVVNDGADEQSEHDEDYFKELSNKKMVNDFLSKGKAAKKKHHSKKKRSGNSLPLHDLDSDDDKEANRKSKKSSKSHIVSASMIDDSDDEYNGEKNMFDSVFFENEQYMRWLLDKHMGSISEEKMKLFGEFTKSRFANKGAITGDWTSLFDGPVPSVVDLELEENHKQDTLENELVRGLSYDEGEDEAGEHIVSPSSPSQAKGKPIDLQQEIQFSDDDYDGEPNSLLAKKRSPAEDSDDSFIVRKRGKPNGLFLDEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHZ1</accession>
    <name>A0A1E5RHZ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1628</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHZ1"/>
    <dbReference type="STRING" id="56408.A0A1E5RHZ1"/>
    <dbReference type="InParanoid" id="A0A1E5RHZ1"/>
    <dbReference type="OrthoDB" id="3973322at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184753" evidence="2">
        <location>
            <begin position="20"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="126"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="458" mass="47303" checksum="9B495E359B4355BD" modified="2017-01-18" version="1">MKFTKNIIAPLLLASRTLADSLVVPIDYDYTIDLYYYFKDGGVQIEPVAEGLKIDLSSNITDVCGTLASGMVVINVDNAPGNTLVTWNQYDVFHLLNEQHQDLTWDSVLVEACGGDTSLETTTLASNTPVSTSGTGSVSTLSATSPRYANSSVSGIEGASLPTVTDIRSYVASLTSAYAVTTPASVAYATGTGKIETFVATVVSSESSSYTTYKQVNSSIATPVLNVVSSSFLNSNSALFSTATPTNALSSTLHADPFDQDASNATTTSVSIAILAPAIQVTTTYSKQATVWETITTCVGHSECTAPSVITTYKIVSASSSAVFSGSLSATGSSTKTTILVSTTLGDENGPSTKIVETTADSANADSSVSASYKPASTTVTTGNASTMHSSKFSTVFASSTASSNGDSSYFSSFGTAAGQSTVGVSVHAQGKAPTNIHNPTQTTFLAFALAFVSFLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI64</accession>
    <name>A0A1E5RI64_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cell wall protein PRY3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1619</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI64"/>
    <dbReference type="STRING" id="56408.A0A1E5RI64"/>
    <dbReference type="InParanoid" id="A0A1E5RI64"/>
    <dbReference type="OrthoDB" id="337038at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01009">
        <property type="entry name" value="CRISP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP" evidence="2">
        <location>
            <begin position="706"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="581"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="876" mass="89410" checksum="21FDFC1DDE6F1C1B" modified="2017-01-18" version="1">MKYSKLIVPTAISSLATAQTTTTENFSAVAFHTAYTKAPLPSSLSSNTASYTTATGSSEYTYGSESSSSETTSTAVTSAFSSAPTNAVPLNSTLTFGVTSETESIFSDTSSAAAGTATITSLQSTQASTVSVSMSTVNNYNSTELQSTTAASEDVNTGTYMTSLSESTPASNSVSFTTSPSSIVSSTQPSIGYNSSSVSSFSADLTSASSIVSSTQPSVGYNSSSVSSFSADSTIASTIVSSTQPSMGYNSSSVSSFSADSTIASSVYISQPYYSSSVESLNTRTSNSSWYYPSSTMSSSSQMLLPPNASISSSDSLTSSFSLSSGLGFITSSPLKFSSSTVASSLLSSQSINNNTLPTLQSSNAYVSSSPMSTSVVSSDIPTGNNSFSGSSSVFTPSASNSISSSIVESSSAAGLSTSSIVESSSAAGLSTSSIVESSSASNSISSSIVQSSSAAGSSVAGSSASSIVQSSSAAGSSSSAMIEPSSVAGSSAFTSLSSAASIVSSSTAVSSSTKAETTPSSSQYFTSASASSSRAVQSTSSSFSSLKIISTSPYLVKSSSSPTTSSTITTSVSSTVKPLVSTSVAKSSSSERSSMLTSSSTSLRSSSTSSSSSLATTTSKTSSAARTTSTTSSSSSSTQTTTSKTTPTTVATSTTSSTTSSTTSSTTSSTTKTTSTPTTSSSTSTNPFVSPTTDIGVYGYLLDQSDSQLLLDLHNSVRSKHVDTPDLVWNNTLVSLAYKYMLFMEEELVSQDACSGVLRHSEGYWRPNIGENLAAMTGNNDLTALFNLWADEAQYYDYDNPSTYGGTDDNTDLGHFTQLVWADTTSVGCGTQICQRSDGSYWTYLICEYYPEGNVLVAGVSDEYELYLENVQPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI89</accession>
    <name>A0A1E5RI89_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein SFK1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1346</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RI89">
        <property type="interactions" value="73"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI89"/>
    <dbReference type="InParanoid" id="A0A1E5RI89"/>
    <dbReference type="OrthoDB" id="10032492at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019402">
        <property type="entry name" value="CWH43_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050911">
        <property type="entry name" value="DRAM/TMEM150_Autophagy_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21324:SF2">
        <property type="entry name" value="EG:22E5.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21324">
        <property type="entry name" value="FASTING-INDUCIBLE INTEGRAL MEMBRANE PROTEIN TM6P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10277">
        <property type="entry name" value="Frag1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="CWH43-like N-terminal" evidence="7">
        <location>
            <begin position="12"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="111"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="125"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="137"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="350" mass="40463" checksum="C4DFF09DFF4B4A08" modified="2017-01-18" version="1">MSILENYKPGNYFFLMPWIGVISWWGMLMALLICWGVQGRPIYSWMQSAEYVHGSGLVYISDIGATNLQPLFIALAGMQGLFYVITLALEYFQRCGRWPFMKHSFVKRHKVSTKESAQQPPKTESQSQVSSSSSSSTLSNTDNAGSATTATHHGDDLQVQAEYLDQQYKQTDYPFYMPFWFSEDERNLMFASFVSACIGEVCILICSIFNTRRHHRVHITMVSLFLLFLAISVILXGTEYILMGMHYRAIHPQHKKNNKYFVSGIWKEVWVCFAILWAILFGAISQNSLSAVFEWLVAFWYGLIFITFSVDFYLGGRYKYSKHFPYVESFQGYYKYDRFYAKYGNDSGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIX3</accession>
    <name>A0A1E5RIX3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Structural maintenance of chromosomes protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g1285</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RIX3">
        <property type="interactions" value="847"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RIX3"/>
    <dbReference type="InParanoid" id="A0A1E5RIX3"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19306">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES 5,6 SMC5, SMC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19306:SF6">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad50/SbcC-type AAA" evidence="15">
        <location>
            <begin position="102"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="RecF/RecN/SMC N-terminal" evidence="14">
        <location>
            <begin position="398"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="50"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="341"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="418"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="724"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="834"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="69"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ86513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1139" mass="130013" checksum="6120DE90274F1327" modified="2017-01-18" version="1">MAEFGNSQIYEDLASLVNTANLPASQRKNLSNLSLHEHLPNKEAARVKLEAHQSLQDVSQQTKKRKTDNASGNMMTQYDTVPSESVXPESVSQTAEAGFIKKVVLKNFMCHENFYMDLGPGLNFIVGNNGSGKSAILTAISICLGAKATTTNRGNSLKDLIKTGRNQSKIQVILNNEGMNAFEPETYGNEIIIERIIKKDGLTQYKIRSENMKEISGKKKTLDNIVDYFSIPVTNPMCFLSQDAARSFLTASTPADKYSHFMKGTLIEETSENHHEIIKKIDSIRNTLQLHQAHVRSLLNDHNAAKSLYQSMRSNVSLLEKKRNLQGKMIWLNVEANERVKGKLTERIQLYEKQIKNCETTIETNNNAICEINEQSVQFENELIDIQTELNKKVVTSKNWEAEVKEKVNQGKIEQGNVETAQEDLNNSRALLDKTNKVYQQKRLQSEKASDENQNKQRAQIEKLAHQIEEVELEHSALASEISSWEEKKASYTEQFSRAKYSLTQSIEKNKSELRQISSSDNDIHNSFHPKMKQALQLIKQYVELGNFKSAPIGPLGLYVTIKEDFAKDWSSAIQNSLNKTLRSFVVSSVDDENKLKSILAKFRIEAQIINRRMKTFDYSMDCPRMKYPTIADALTFDNREVECTFVDLNSIHTVGLIADRHVARKVLKSGVRNLRMVLSIKTQNSIFRSSAYGSXFSVDTINNISPVVTMKTVAQQDYGAYYRSAIQEEEAQLNELKQKHNDQLREATAELKRLLGQKNNLFNKKRYWIDEKTNIELELKKVVDAADLEMLQQQIETQQETISLNERVIEELKFKLAEILDEISPVEKSYIAAKEEQQLVANAYNEKKRLLNKNSVEVSNQQDAIVREQRNITKLKNRIDQYETELETMENGIGKVLEAAEAHCPREVADALDVGASKEEVEEELHRVERQIKVSEKNLGMSHEEGRELYIQTKEKFDIARKRVHELEEGVDYLATSVQSRLDALSIAQKATCLNADVDFKRSLRFRDFSGSLDFDNEKKALRVFVQTRNDVTARDVDTLSGGEKSYSQISLLLATWKPMKSRVIALDEFDVFMDQVNRTIGTRLIMFKLSSNLRMQTIIITPQDISKIANLEEQKNVQIMRMTDPERQNNSAFHNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN93</accession>
    <name>A0A1E5RN93_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein uS3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">40S ribosomal protein S3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g674</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN93"/>
    <dbReference type="FunCoup" id="A0A1E5RN93">
        <property type="interactions" value="1219"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN93"/>
    <dbReference type="InParanoid" id="A0A1E5RN93"/>
    <dbReference type="OrthoDB" id="10248446at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02413">
        <property type="entry name" value="KH-II_40S_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1140.32:FF:000013">
        <property type="entry name" value="40S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.20:FF:000006">
        <property type="entry name" value="40S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="entry name" value="Ribosomal protein S3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057258">
        <property type="entry name" value="Ribosomal_uS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_uS3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018280">
        <property type="entry name" value="Ribosomal_uS3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005703">
        <property type="entry name" value="Ribosomal_uS3_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003219">
        <property type="entry name" value="PRK04191.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01008">
        <property type="entry name" value="uS3_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760:SF32">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="Ribosomal protein S3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00548">
        <property type="entry name" value="RIBOSOMAL_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8 11" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="KH type-2" evidence="10">
        <location>
            <begin position="23"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ88359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="241" mass="26733" checksum="E23BC7A1ABBB5DAC" modified="2017-01-18" version="1">MSQRPSISKKRKLVADGVFYAELNEFFTRELAEEGYSGCEVRVTPTKTEIIIRATRVQEVLGENGRRINELTLLVEKRFQYPKGAIVLFAERVQDRGLSAVAQAESLKFKLLNGLAIRRAAYGVVKFVMESGAKGCEVVISGKLRAARAKAMKFQDGFLIHSGQPVNDFIDTATRHVLLRQGVLGVKVKIMRDPARARVGPRSLPDAVKIQEPKEEAIIEQPSVKSYRQEAPKEAPVAEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RND9</accession>
    <name>A0A1E5RND9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">37S ribosomal protein S25, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g848</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Component of the mitochondrial small ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RND9"/>
    <dbReference type="FunCoup" id="A0A1E5RND9">
        <property type="interactions" value="164"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RND9"/>
    <dbReference type="InParanoid" id="A0A1E5RND9"/>
    <dbReference type="OrthoDB" id="5542239at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016939">
        <property type="entry name" value="Ribosomal_mS23_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37799">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37799:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13741">
        <property type="entry name" value="MRP-S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029764">
        <property type="entry name" value="RSM25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="247"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="247"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="257"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR029764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ88396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="271" mass="32204" checksum="0784161E61B55738" modified="2017-01-18" version="1">MKFQRDAVSILQRTSDFLEKKVINEKPKWYDVVMRTPPNTQFSRVPKFENNPSHNKPLYKDQVASSSLQNLKSELYKSSQSYKPFFYKTRPSQKDKCLSDAHLYKPYKLKYFEDKLREIFYDQHPWERSRPKLMIENTKVLDAKLDWSTMKQLTKPLDGESVIQRTLYLVETEGATLSEAYELAKFEFYQLRIEEEFENQIAREEAELHGALFRETAVEFGFNEEQKYIKYWKKKAIEKTELLQAQSSDNSGFTTGKNREDEAEKTEEHVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNQ2</accession>
    <name>A0A1E5RNQ2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Tyrosine-protein phosphatase-like protein OCA2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g817</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNQ2"/>
    <dbReference type="FunCoup" id="A0A1E5RNQ2">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNQ2"/>
    <dbReference type="InParanoid" id="A0A1E5RNQ2"/>
    <dbReference type="OrthoDB" id="6375174at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052840">
        <property type="term" value="F:inositol diphosphate tetrakisphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000035">
        <property type="entry name" value="Tyrosine phosphatase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004861">
        <property type="entry name" value="Siw14-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF74">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE-LIKE PROTEIN OCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03162">
        <property type="entry name" value="Y_phosphatase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ88490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="193" mass="22287" checksum="785F4E0FEF6F8461" modified="2017-01-18" version="1">MDDKIDGNATRKETHCHESPTIYIPPLNFAPIVNSSQDIIYRSGFPMPLNYPYLLNTLNLKTIIYLGDKVDLLPQYTEFIEKGEYQFHHLLMNSTTDKNIQELMEEMLELVLDKRNYPILIHSNKGKHRIGVAAGIIRKILQGWSITGIYQEYGIFTGNVKGEMDLEFINSFCVRNPLKVDPKFLPSFINFET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP44</accession>
    <name>A0A1E5RP44_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Binder of USO1 and GRH1 protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g939</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP44"/>
    <dbReference type="FunCoup" id="A0A1E5RP44">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RP44"/>
    <dbReference type="OrthoDB" id="4036611at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="285"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="197"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="96"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="344" mass="39000" checksum="46AFBB55A56AEFC5" modified="2017-01-18" version="1">MSEVEGQPEKQPVEQSPSEDVVIEKTTEKFPEQNNDGNSETAEAGDKEEQERLKKLEEARKRVEALRNRKKNKKSKKKDTAASTEETSTEGTPIPDSEHSEKAKTTEQVDEVDQKLTKEEDLMKDKNGEEVTTSVDKPAETTVKDELEQKVEGVEEVKEPVHDELDELFGKVEHKTGASVSLGRAEATESFLATIQKEQESFELEQYKTKVSDLEQQVKKLKFDNMEYETTIDDLHDEVSELKAQLARTQKELQELKSKNTAAKPALSTNSSTLQLSSFNTAQQQNSYQPSSLYTPQSYGNLPQQEHNTPVQPVDQELLYSKWKDWNVDMTSWRSIGTGPIVQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV15</accession>
    <name>A0A1E5RV15_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative sugar kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g396</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FGGY kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RV15">
        <property type="interactions" value="294"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV15"/>
    <dbReference type="InParanoid" id="A0A1E5RV15"/>
    <dbReference type="OrthoDB" id="203824at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019150">
        <property type="term" value="F:D-ribulokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019321">
        <property type="term" value="P:pentose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07782">
        <property type="entry name" value="ASKHA_NBD_FGGY_D-RBK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006003">
        <property type="entry name" value="FGGY_RbtK-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01315">
        <property type="entry name" value="5C_CHO_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43435:SF4">
        <property type="entry name" value="FGGY CARBOHYDRATE KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43435">
        <property type="entry name" value="RIBULOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="4">
        <location>
            <begin position="40"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="5">
        <location>
            <begin position="338"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ90787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="733" mass="79864" checksum="EC19DAE4B737E6EA" modified="2017-01-18" version="1">MARRPSHSSLSLLQKQLIEEQAENYDHQNLLKNHPELNQYYVGVDVGTGSARACIIDSTGNILAMSERAIIKEILKPNFITQSSTEIWNAICHCVKSVVRESEIDPYKVHGIGFDATCSLVVVDENTGAPVGAGXDFTNDNQNIILWMDHRAMDETAKINSTNHKVLKYVGGQMSVEMEIPKIKWLKDHMAKETYGKCEFYDLPDYLTFKATNQKNRSFCSTVCKQGLLPPGVEGSIKGWSQDFLTEIGLEDLTANDFQKLGGDASKTFKSAGEVVGVLSPESALQLGLTTNCVVGSGVIDAYAGWVGTVAAKTDFDLDPLVHLDNNEKGIAKATGRLAAVAGTSTCHIAMTKDPVFVNGVWGPYRDVMAKDFWLAEGGQSCTGALLAHVLSTHPAFTELATQSDTANVSRFDFLNSRLEXLTAQKGARSVVALAKHLFFYGDYHGNRSPIADPNMRAAIIGQSMDNSIDDLAILYLASCEFIAQQTRQIVEKMCASGHEIKAIFMSGGQCRNGLLMRLLADCTNLPIVIPRYIDSAVVFGSALLGAVASKCYDKDPFKKSTNAERRASLTEEQPPLPTKSHFAQFAKAAGYDLNEGAHSPSPYTMPTASSTSGVSSGFPFPHVTPASQMALNKKTSAIAEEEEVTSPKTSTSNEALSFNSVNHQKSVSDLLAGDLLWDVMYNLTGAGKVVKPNSSDHPDRKLLNAKYEIFLDMAKTQRDYRTKVDELEKQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVE9</accession>
    <name>A0A1E5RVE9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g525</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVE9">
        <property type="interactions" value="130"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RVE9"/>
    <dbReference type="OrthoDB" id="4069217at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054776">
        <property type="entry name" value="VIR1_yeast"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22575">
        <property type="entry name" value="Vir1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ90864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="764" mass="88730" checksum="78F8612E2983D01C" modified="2017-01-18" version="1">MMGGSSSRCPLKEEVIKVIPLVFTKIHGFVLQLDKPEEELDXDVVESVXXCXKEMLYEGVNLLRDVSVMYDEGNLKELYWTGDKFKENEXMVRLLIEKRDYYDDGYILGMFIACLCCHKEFHMFLSLKNFQDLKNMPISTDTVMNGKPTKKXDSGMFDAFEFLQNTHKDVSFENISQYGXPKFSEMFIANLQLCTASNMVNTSTLMENVNNNWFLDWCILNHLDDFFKAGESNVENSQCHLYFKFKMIKRSIEFPRCFNASTFQKTIWPEYGKLIKNENLIIDKYHSSTDPCHKDIHLWLEVLLEIIDHSREIDFFDSSDHLITILNTSVKCLNEYILSKNVLDIHYVLNHDLQSTLTFPAILNIVTYFLLKQLIQNNYYVNADEXNAHPDVLHLLQFDQQMIPSIPPICKSMFQFDYNDIELPSETRNMKSQYGKVVRCLXNCQNLLLNVLDIYKISFGIDIFQNSCHFPLDKTSSMKTAPESIYYKECELFSKNFFIPLMTSLLLLNHFKSYTSEIELLYCNDKSSLKVWGNLIFGNVLQLVLNVVGFENATPQVETKNFSDNINTSAITLYNFIKFSKEVCVQTTQLHQPLLQTLNAILTPENYEMFIQHNPLTKNELLELLNSCFYGSDSRVVLRIYEMMNVEAPNVTNEHDVKLKDFLRNLLHDETDLNKIDQLFQAAKNSKPVNEDAYSRTMSGAYLNNTENQSHNNNNTYPAYNYNTDSQSRKLSIVDSGKKYILGGHHRLTNTSRVQSVHIDDYYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZ53</accession>
    <name>A0A1E5RZ53_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">H/ACA ribonucleoprotein complex subunit CBF5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">H/ACA ribonucleoprotein complex subunit cbf5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g122</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a uridine in mRNA = a pseudouridine in mRNA</text>
            <dbReference type="Rhea" id="RHEA:56644"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:14659"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>uridine in 5S rRNA = pseudouridine in 5S rRNA</text>
            <dbReference type="Rhea" id="RHEA:47036"/>
            <dbReference type="Rhea" id="RHEA-COMP:11730"/>
            <dbReference type="Rhea" id="RHEA-COMP:11731"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>uridine in snRNA = pseudouridine in snRNA</text>
            <dbReference type="Rhea" id="RHEA:51124"/>
            <dbReference type="Rhea" id="RHEA-COMP:12891"/>
            <dbReference type="Rhea" id="RHEA-COMP:12892"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the pseudouridine synthase TruB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZ53"/>
    <dbReference type="FunCoup" id="A0A1E5RZ53">
        <property type="interactions" value="1615"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZ53"/>
    <dbReference type="InParanoid" id="A0A1E5RZ53"/>
    <dbReference type="OrthoDB" id="10250002at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000495">
        <property type="term" value="P:box H/ACA sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031118">
        <property type="term" value="P:rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02572">
        <property type="entry name" value="PseudoU_synth_hDyskerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21148">
        <property type="entry name" value="PUA_Cbf5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.10:FF:000001">
        <property type="entry name" value="H/ACA ribonucleoprotein complex subunit CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012960">
        <property type="entry name" value="Dyskerin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004802">
        <property type="entry name" value="tRNA_PsdUridine_synth_B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00425">
        <property type="entry name" value="CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003280">
        <property type="entry name" value="PRK04270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127">
        <property type="entry name" value="CENTROMERE/MICROTUBULE BINDING PROTEIN CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127:SF0">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT DKC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08068">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01136">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Dyskerin-like" evidence="10">
        <location>
            <begin position="19"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="PUA" evidence="9">
        <location>
            <begin position="268"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="424"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="424"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="448"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="480"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00072225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ92280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="490" mass="55262" checksum="67781376FDBF061A" modified="2017-01-18" version="1">MSKDQDYVIQPESRAPTTNSNEWPLLLKDYDKLLVRSGHYTPIPMGASPLKRDLKSYISSGVINLDKPSNPSSHEVVAWVKRILRCEKTGHSGTLDPKVTGCLIVCVDRATRLVKSQQGAGKEYVCIVRLHDALKEEKDVYKGLENLTGALFQRPPLISAVKRQLRVRTIYDSKMIEFDNKRNLGVFWASCEAGTYMRTLCVHLGMLLGVGGHMQELRRVRSGAMSENDNLVTLHDVMDAQWVYDNTRDESYLRKIIQPLETLLVGYKRIVVKDSAVNAVCYGAKLMIPGLLRYEEGIELYDEVVLMTTKGEAIAVGIAQMSTVDLATCDHGVVASVKRCIMERDLYPRRWGLGPIATKKKQMKKDGKLDKFGRTNENTPEDWKKTYVSLETAPATEKVAVEEVAVSKPTEDDVKAEIKETITVVEKTDAADEESISKSKSGKDKKEKKDKKEKKEKKDKKDKKEKKRKASEEDESSEKKKSKKSKKSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZ98</accession>
    <name>A0A1E5RZ98_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein RSN1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g262</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000002">
        <property type="protein sequence ID" value="OEJ92156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZ98">
        <property type="interactions" value="171"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RZ98"/>
    <dbReference type="OrthoDB" id="1076608at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022257">
        <property type="entry name" value="PHM7_ext"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF26">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G10920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12621">
        <property type="entry name" value="PHM7_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="383"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="427"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="474"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="520"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="570"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="595"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="633"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="661"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="11">
        <location>
            <begin position="18"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="12">
        <location>
            <begin position="191"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="9">
        <location>
            <begin position="380"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="domain" description="10TM putative phosphate transporter extracellular tail" evidence="10">
        <location>
            <begin position="811"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="898"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="905"/>
            <end position="916"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ92156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="942" mass="106596" checksum="16E51E81B7E5090F" modified="2017-01-18" version="1">MAKSKSEGSSAGTSTQQVLTAIITNGVIFAVFVSIFLLLRLNKKFKRIYAPKSSFNLINDEKKPDPLPTGLWQWFLPLLKKSDNFVLQQAGIDGYFFLRYLFIISMYCLFSMCYMFPILFPVNAANGHRLKGLDQLAYSDVKHSSRYYAHTFLSWIFFWGFLFVVYRELYFYTSMRQAILSSPRYAKKLSSRTVLFQTVPQDYLMENDFNKLIVGAKKIWIARADPELVNKVNERQKMAMKLEGTLTSFITSTVKKINKKKLNENGTHSISNNIMAYNKKAPQHRLKFLIGEKVDTIEYLKAELPKIDEEVKVLQSKHDEAKPWNSVYVEFESQYQAQMAYQSIPHSHALRLNPIYIGIEPNDIVWFNMRMFWWERLGRKTGAAAAIVALVILWAFPVAFVGMVSNVTYLTEKLKWLRWIDNMPKPLLGIITSLAPTVALAILMSLLPIFIRKMAVTAGSPSAPYIEYFTQQSYFAFQVIQVFLITTLASSATSTVTEIVKEPTKAMSLLASNLPKSSNFFISYTILQGFSIAGGALAQLVPLILFYVLGYFLDKTARKKYTRFSNLSSMSWGTTFPVYTNLAVIIFSYAVISPIILLFGFVGFLLLYIAYLYSLTYVCQESPDGRGMYYPRALFQTLVGVYIGQVCLLGLFVVGKGWGPIVLEVVCICVTVFIHTNLSAAFDKLNTVLPVDCMKPLDGKSNTPSFVNIYNNHKSHRNGVVSTGDDNSLSMERDDIKELPKFQVKKYQPRLNSYGGKDQQTSVLTDYTFEVQQQSTHGLPFNDNENNIVSVPLLADGDTDPMPPAPAWKRFFYPHVYAGYKAVKSKLPNIYQLKEPNEIADTKHAYDLPAVAEQCPIIWIPKDPYGFSTVFIEELAKCKIQASDDGAFVNEQGKLVWETDSVPPDSDDYIEPEAQDSDMKXDSLDIDSTGENTSADKANPFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZG0</accession>
    <name>A0A1E5RZG0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Chromatin-remodeling complex subunit IES6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g198</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZG0">
        <property type="interactions" value="77"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZG0"/>
    <dbReference type="InParanoid" id="A0A1E5RZG0"/>
    <dbReference type="OrthoDB" id="49520at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029525">
        <property type="entry name" value="INO80C/Ies6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013272">
        <property type="entry name" value="Vps72/YL1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31200">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31200:SF1">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08265">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00993">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Vps72/YL1 C-terminal" evidence="6">
        <location>
            <begin position="129"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ92362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="181" mass="20605" checksum="EA1C99C6DC50CCFF" modified="2017-01-18" version="1">MDKIDFIRECIEHNNKNNATKHKLKSLKWLNHTNKSSLDKKNSAPAPVKKGRKPSNFTKRIVSKSARQVLSDEWKRINNLNNVDGVDGQENGTASGXNKEKSLEAAQKQRNEVTYFNVEAPPSLLPVKRYCDITGLKAVYRAPSNNLYYHNAEVYTNVIKPMLPGNDQQYLKLRKADVVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZG3</accession>
    <name>A0A1E5RZG3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Electron transfer flavoprotein-ubiquinone oxidoreductase</fullName>
            <shortName evidence="19">ETF-QO</shortName>
            <ecNumber evidence="19">1.5.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">AWRI3579_g216</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 19">Accepts electrons from ETF and reduces ubiquinone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 19">
            <text>a ubiquinone + reduced [electron-transfer flavoprotein] = a ubiquinol + oxidized [electron-transfer flavoprotein] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24052"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.5.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="19">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ETF-QO/FixC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.5.1" evidence="19"/>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZG3">
        <property type="interactions" value="685"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZG3"/>
    <dbReference type="InParanoid" id="A0A1E5RZG3"/>
    <dbReference type="OrthoDB" id="437331at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004174">
        <property type="term" value="F:electron-transferring-flavoprotein dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.20:FF:000015">
        <property type="entry name" value="Electron transfer flavoprotein-ubiquinone oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040156">
        <property type="entry name" value="ETF-QO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049398">
        <property type="entry name" value="ETF-QO/FixC_UQ-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007859">
        <property type="entry name" value="ETF-QO/FixX_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10617">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN-UBIQUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10617:SF107">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN-UBIQUINONE OXIDOREDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21162">
        <property type="entry name" value="ETFQO_UQ-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05187">
        <property type="entry name" value="Fer4_ETF_QO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9 19" id="KW-0274">FAD</keyword>
    <keyword evidence="6 19" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13 19" id="KW-0408">Iron</keyword>
    <keyword evidence="14 19" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5 19" id="KW-0813">Transport</keyword>
    <keyword evidence="15 19 21" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="20">
        <location>
            <begin position="614"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="21">
        <location>
            <position position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00052682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU366068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OEJ92341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="654" mass="72128" checksum="A24A880C93AB2DAD" modified="2017-01-18" version="1">MINTSVRRSVARFGARQVRSAAPRHRIVANSARFCSIAQLNNLISGKKQFHTCAPAFQSVETPFSKLSEDQQALLTEERAVDRADVVIVGGGPAGLAAAIRLKQLDAKHNGNLRVVVLEKSSEFGNHTVSGVILEPKALRELFPEECEGKPATYIPLPENLXTPVTSEGLKFLFQEFGASVPVPEPPAMVNKGKNYIASLSQVVRHLSECAENLGVEVYPGISVSELVFDKAKENVIGVATKDMGVSKKGEPKDSFERGMEFHARQVFLAEGCHGSLTKKAIKQYDLRKDKQQQSFGLGVKEVWEVPPENFKKGYVAHTMGYPLSNSVYGGGFQYHFGENMVTVGLVVGLDYKNPYVSPYQEFQKMKHHPFYSKVLKGGKCISYGARALNEGGYQAIPKLHFPGGALIGASAGFMNVPKIKGTHTAMKSGMLAAESIFDILKNEETSFSELEDESAIGALPTIDLKSYEDNFKXSWAHDELFEVRNIRPSFNTKLGNLFGMCWSGLDSFVLKGRPGFSFKFHETGDSGITETASQHKPIDYPKPDGVLSFDILTSVSRTGTYHDEDEQCHLRVPDQDLEKHANASYPKYKGIEQRFCPAGVYEYVEDPESPIGVKFQINSQNCIHCKTCDIKVPTQDIDWVVPEGGDGPKYTIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZM0</accession>
    <name>A0A1E5RZM0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Mitochondrial division protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g9</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZM0">
        <property type="interactions" value="102"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZM0"/>
    <dbReference type="InParanoid" id="A0A1E5RZM0"/>
    <dbReference type="OrthoDB" id="496at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22881">
        <property type="entry name" value="Mdv1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="390"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="430"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="509"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="532"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="278"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="219"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ92422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="667" mass="74304" checksum="410E25C908EB658C" modified="2017-01-18" version="1">MSDISNFTKAVTATATALFNHGSDGELLSSDSSYKTMLKDTLKQSESTLSNSIILLKKSSKGSSNKTQSTFLDLFNSENGKGYFRMNGKRSNEKANFRVMSYISDDLLHDIPSEHKSGSRSAGDRKLLKDASANDEDGNEQNMEPSLFQGFEATLAQPQKITDAKQHGGPLLLDAKGNTTLSLPKNFDIQKLQKTYSLSILNKGQTQLNDHLALLEIQKKIAATEISELDLKIKQLKDKRKVVFQRVATIEENESFVEKNMAILVERMDMIKEYNLDSESPLAGNESGQDDDDDNDTKNESNXGGEGREEIPKLAVTKESPKSPYKRKIQPTLQNFYQPNSLIHSFENLMATPIISLEFDVPFGTMCCAGEGERVVEVWDLQSHEKQGVLNGHLASVTCMQMEDQLLITAGRDAQIKLWNLNTMECVETLDSHIEEITAMSFDGETIVSGSQDRTLRQWDMQSGKCVQTIDMNFIKSAKPITSLSPKNPLLLAHEQPVIGAVQVFDAALATGTKDGIVRLWDLRSGQVVRTLEGHTDSVTSLKFDSSDLVTGSLDKTCRIWDLRMSTLKDLMKFEEPINNIELDPSKIVIGVDSHKRAKIYDRVKKELLDYTEITENFYSNSALDESSASETEEQESDENKFVTAIRYKDGYLLEGYANGCVSSLAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZQ4</accession>
    <name>A0A1E5RZQ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Manganese-transporting ATPase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g192</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZQ4">
        <property type="interactions" value="1137"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZQ4"/>
    <dbReference type="InParanoid" id="A0A1E5RZQ4"/>
    <dbReference type="OrthoDB" id="48943at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015662">
        <property type="term" value="F:P-type ion transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07543">
        <property type="entry name" value="P-type_ATPase_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000071">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057255">
        <property type="entry name" value="2TM_P5A-ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047820">
        <property type="entry name" value="P5A-type_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01657">
        <property type="entry name" value="P-ATPase-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF7">
        <property type="entry name" value="ENDOPLASMIC RETICULUM TRANSMEMBRANE HELIX TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23143">
        <property type="entry name" value="2TM_P5A-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01229">
        <property type="entry name" value="COF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="194"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="217"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="395"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1056"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1136"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1175"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="domain" description="P5A-ATPase transmembrane helical hairpin" evidence="14">
        <location>
            <begin position="18"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="13">
        <location>
            <begin position="254"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ92364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1208" mass="135470" checksum="19009CB01ECE63A8" modified="2017-01-18" version="1">MVEVKSSLVQDSKLLKSRPLLARPYVFPFIPLYAFFYRXYTQLYDEYIGGQEWTFVYLGSIISLNLLFWLMPAWNTSINLLFNYSKVSSLEEAEFVYVKTTPNNGSDDIVKIESAFEQNTVQIFFQFQKKRFLLDDAKQTFASPKFSIDEPLKLEFFQKNKGNSGDLTHARRLYGENTFDIPIPTFLELYKEHAIAPFFVFQVFCVALWFLDEMWYYSLFNLFMILMMEAATVFQRLNTLKEFRTMGIKPFPINVYRDGKWTLLQTNELLPNDLVSVVRSNDETAVPCDLVLVDGSCIVNEAMLSGESTPLLKESIKLRPQNEFIDLEGVDKNSILHGGTKCLQITKPDSPIVKQAPDDGALAIVTKTGFETSQGSLVRVMLFSAEQVSANNKEAFAFIGFLLIFAIAASWYVWVEGTKMGRVHSKLILDCVLIITSVVPPELPMELTMAVNASLAALSKFYVYCTEPFRIPLAGRVDVCCFDKTGTLTGEDLVFEGVAGLKSEDGNELIQPDASCEDTSFAIGSCHALVKLDDGEIVGDPMEKATLKACGWTVESETKVSHQELGTAKVLRRFQFSSALKRSATVSSYKNKVCVSVKGAPEVLRERLSSIPSNYDDIYKHYTRNGSRVLAIAVKTLDSNIKIDKLIRQEVESGLKFVGFLVYHCPLKDDAVETIKMLNESSHRSIMITGDNPLTAVHVAKQVAIVERDVLIIDKPSESHEIDYSADDLIIRNVEETFIKKFTPSVDEFEGIFGKYDLAVTGYALNLLFGHKQIKELLKHTWVYARVSPTQKEYILNELKDMGYQTLMCGDGTNDVGALKQAHVGIALLNGTEEGLKKIAEQRKIDGMKQMYDKQCDMFNRWGKPAPPVPEPIAHLFPPSQLNPHYLKALEKQGTEITPEMRKEVDKAMKQPLKAKENKPLRPEDIKSGDMSDLFMNALNGAGGDDDEDSPVLKLGDASCAAPFTSKLSKVSAVTNIIRQGRCALINTIQMYKILALNCLISAYSLSIMFIMGVKFGDAQATVSGLLLSACFLSISRGKPIEKLSKERPQAGIFNVYIMGSILSQFAVHCISLLYLTNEIYKLEPREPVLDLDKTFEPSLLNTGIFLIQLIQQVSTFYVNYQGEPFRENIRNNKGMYYGIVGVFALAFAGATELIPELNEAIKFVPMEDSFKGKLTGALLLDFFGAWAGEQFFKFFFMDDKPSDIAHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZU5</accession>
    <name>A0A1E5RZU5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Flavin carrier protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g233</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transient receptor potential (TRP) ion channel family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZU5"/>
    <dbReference type="FunCoup" id="A0A1E5RZU5">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZU5"/>
    <dbReference type="InParanoid" id="A0A1E5RZU5"/>
    <dbReference type="OrthoDB" id="5212126at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010308">
        <property type="entry name" value="TRP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040241">
        <property type="entry name" value="TRP_Flc/Pkd2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032800">
        <property type="entry name" value="TRP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145:SF2">
        <property type="entry name" value="FLAVIN CARRIER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_7G01610)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06011">
        <property type="entry name" value="TRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14558">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01320">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184961" evidence="9">
        <location>
            <begin position="28"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="389"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="445"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="478"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="534"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="561"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="590"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="622"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="ML-like" evidence="10">
        <location>
            <begin position="66"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="704"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="743"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="775"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="775"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="788"/>
            <end position="818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ92471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="825" mass="92114" checksum="AAAE13AE1D5CF2D3" modified="2017-01-18" version="1">MPKSNFLLKLVFSLFILLQIFNPLTYADDDTTATTTTGAATTTKADDSVLGISTSTTNVKNKDPSKLLRTSSLLTCMENSQFSSSFFDVTYFPGNTSVVFNIEAVTTISSKVEIEFQVIAYGLNIIQRSINLCSLNEKSLCPLSAGRIDISSTYQITSDVVDNIPSVAYTVPDLDALVRVYAYSLDDDGNADTSDPVACVLAPLTNGKTVQTKYASWPIAAISGVGVLTSAFYSVMGHSATSAHIASNSISLFVYFQNLAITAMMGVAKVPPIAAAWTQNFQWSMGIINAGFMQKIFKWYVQATGGTSTNILANKSILSIYVQKLKNKLKHKLAKRAITLSGSFDDSVFDDTSVYTTNERNITAVASKTLLVRGIERVAYKADIELSNFFLTGIVFLLFFIFCMCLLLFCLKSLLELLVRTKAMNPDNTKFFEFRKSWGNIIKGTMYRIALIAFPQVTLLCIWEFTQQDSVACMVDSAVILLIILCLMVYGTTRVVIKGLQSSKIYKNPAYLLYGDSEFLNKYGFLYVQFKATAYWWLVPLISYSLLRSLFVAALQTKGKAQSLIVFIIELFYFVGLCVFRPYMDKRTNIFNISIHLINLLNSIFFLFFSNLFGQPMIVSSVMAIVLFVMNAVVALYLLVFTIVTCVLAVLHKNPDSRYQPMKDDRVSFIPRAGGMATMDNKAAELNDLSKAVMVTNETENQKLQRDNAFQLGSQSDSSKRKLLMDDDNEDYELESNSIWSKKDSFGRQQQTQSDDPDVVTRSESYLDVQQPLSSLNNQNHSQQPPLSNSAQYYSQTNNNNNNPYQNFTSNNRNTNNNPYSSGYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZW3</accession>
    <name>A0A1E5RZW3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein bL32m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g133</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bL32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZW3"/>
    <dbReference type="FunCoup" id="A0A1E5RZW3">
        <property type="interactions" value="295"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZW3"/>
    <dbReference type="InParanoid" id="A0A1E5RZW3"/>
    <dbReference type="OrthoDB" id="2014905at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051991">
        <property type="entry name" value="Mitoribosomal_protein_bL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002677">
        <property type="entry name" value="Ribosomal_bL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01031">
        <property type="entry name" value="rpmF_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21026">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21026:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL32M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01783">
        <property type="entry name" value="Ribosomal_L32p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ92298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="248" mass="28280" checksum="4A45FE2B78E19045" modified="2017-01-18" version="1">MALSIPIDQISIQPASVHWLNFQHKNAGLKTRYIESMSAGVLSLRNVLAPRASAVWWPSALSKIVSKPFLSAPALQSSAITSDIGISLPLGKLKEMLGISDCVHHDHADHQHLEKDFFSNNGILKAVPKKKMSLQKRRSRQLGPARKQEKYINHLNRCPSCGHYKRSHTVCMNCFVEVRDLWTKFMGKPAKSEPLQNEYLTKEDKNVLYSNRKPMDFEKSALRRKNINEKNRLMKRNTTNTLPVEPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S041</accession>
    <name>A0A1E5S041_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Succinate dehydrogenase assembly factor 4, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g40</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SDHAF4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5S041">
        <property type="interactions" value="83"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S041"/>
    <dbReference type="InParanoid" id="A0A1E5S041"/>
    <dbReference type="OrthoDB" id="201362at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012875">
        <property type="entry name" value="SDHF4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28524">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28524:SF3">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07896">
        <property type="entry name" value="DUF1674"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="110"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ92456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="142" mass="16103" checksum="D69E491E216CD2EE" modified="2017-01-18" version="1">MLSRFHPPKAFQRLSVSSVLSPLSRRAPVIQFHRTLFGQETPAGPPPLPKEDQEEFERLQKLAQTSDIIESYNELAXENEDKEKIKQLLKTKQTSDIGQINVFKTIPEFEGDVNPETGEIGGPKQDPLRHGDYSFNGRVTDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S043</accession>
    <name>A0A1E5S043_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UV excision repair protein RAD23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g188</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Involved in nucleotide excision repair.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RAD23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5S043">
        <property type="interactions" value="1014"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S043"/>
    <dbReference type="InParanoid" id="A0A1E5S043"/>
    <dbReference type="OrthoDB" id="419317at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14281">
        <property type="entry name" value="UBA2_Rad23_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000002">
        <property type="entry name" value="UV excision repair protein RAD23 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000003">
        <property type="entry name" value="UV excision repair protein RAD23 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.540">
        <property type="entry name" value="XPC-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004806">
        <property type="entry name" value="Rad23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015360">
        <property type="entry name" value="XPC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036353">
        <property type="entry name" value="XPC-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00601">
        <property type="entry name" value="rad23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10621">
        <property type="entry name" value="UV EXCISION REPAIR PROTEIN RAD23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10621:SF0">
        <property type="entry name" value="UV EXCISION REPAIR PROTEIN RAD23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09280">
        <property type="entry name" value="XPC-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01839">
        <property type="entry name" value="RAD23PROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101238">
        <property type="entry name" value="XPC-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3 5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="4"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="7">
        <location>
            <begin position="143"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="7">
        <location>
            <begin position="360"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="78"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="193"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="107"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="193"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="230"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ92343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="407" mass="43297" checksum="E4419E540B3CD9CB" modified="2017-01-18" version="1">MSSITVKFKDFKKNIHPVSLGADSSVEETKQKLCDTLGNNTTLDQIKLIYSGKVLVNSKSLSTYNIKDGDQIIFMVSKKKQATPQPQEQTKEETKVTDPILSANEQSTTTPASEPTPAAEPAAEPAPASEGAPAXAANNTIAPPDEYITRLMELGYSRDESVMALQLANNDPNRACELLLMGLTEEMVAGATEAASGAAPQGHVEPQQDGEDDHDMEQESGADDADGDDLFAQAAAAAGGDSNXAGAGARGAGARGGNAPRSISLTMEDLMQLRRIVNGDPESLQPFLENMISRYPQLEDQIQSSPEMFLAMLLEGLGGNIPGDLDEQMGAQEGEAGALGDSAAATNLPGQAPQNTIQLTQEENDAINRLCELGNFDKNLVIQIYFACDKNEELAANLLFTEHSNFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R024</accession>
    <name>A0A1E5R024_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Eukaryotic translation initiation factor 3 subunit G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4639</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000017">
        <property type="protein sequence ID" value="OEJ80249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R024"/>
    <dbReference type="FunCoup" id="A0A1E5R024">
        <property type="interactions" value="1115"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R024"/>
    <dbReference type="InParanoid" id="A0A1E5R024"/>
    <dbReference type="OrthoDB" id="639027at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017069">
        <property type="term" value="F:snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017334">
        <property type="entry name" value="eIF3_g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024675">
        <property type="entry name" value="eIF3g_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051183">
        <property type="entry name" value="U1_U11-U12_snRNP_70-35kDa"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN 70 KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952:SF6">
        <property type="entry name" value="U11_U12 SMALL NUCLEAR RIBONUCLEOPROTEIN 35 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12353">
        <property type="entry name" value="eIF3g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037949">
        <property type="entry name" value="Transl_init_eIF-3_RNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="187"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="270" mass="30416" checksum="9EED7915ED295878" modified="2017-01-18" version="1">MSAVANAPVDFEKQEIIKNDDGTKTIISYKLEKGVKYKITQKVKEVKIIEKTNPAIEERKKWAKYGVAVSDTKDLLTVGDDVPFFLGVNWKEMQEQYENKKNKKTVKSFVCSNCGGAHLSAQCPFPKKSSATAAASAASGAGAVPASTGKYVPPSKRALLAANAKAAASGEAILTQEEMRKKEEDERTLVFADLNLNTTEDDLTREVLKYFQYERCRLVRDRNTGRSRGLAYVSFYTKEEAERAANYWNNKGYMNIMMRVRFVQEHRQNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0L1</accession>
    <name>A0A1E5R0L1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4610</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0L1">
        <property type="interactions" value="310"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0L1"/>
    <dbReference type="InParanoid" id="A0A1E5R0L1"/>
    <dbReference type="OrthoDB" id="18472at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05600">
        <property type="entry name" value="STKc_Sid2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000141">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000319">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000109">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF417">
        <property type="entry name" value="CELL CYCLE PROTEIN KINASE DBF2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="185"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="12">
        <location>
            <begin position="485"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ80435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="579" mass="67021" checksum="F8169CB0008599AA" modified="2017-01-18" version="1">MSFFNSPTRKSQYTRENVDALAGNISNLSFSNNADGSAQPITKVNHQRKELFTPTKNTNYNRYYGETTTPVAGDLSLMEIDEDGAEQAAXKDISNSPRKLPQDFHSKVSTTKTKRLVDVCQMFFLDYYCDMFDYVISRRERTREVALMLKDQPNPQDAQSAWQGYFQKETYALRKRRLKPRTKDFEIVTQIGQGGYGAVYLARKKDTSEVVALKTVNKRYFSKETNYFLTERDILTITRSEWLVKLFYAFQDSSCLYLAMEFVPGGDFRTLLFNTRTLISAHARFYISEMFCSVNALHELGYTHRDLKPENFLIDARGHIKLTDFGLAAGHVSKERIESMKIRLEQVRHLDFPEFKERSMEDRANMYQQLRSENVNYASSTVGSPDYMALEVLEKGRSYDFTADYWSLGCILFESLVGYTPFCGGNTNETYENLRNWKTVLKRPVKSNGQYAISDRTWELITRLIADPTKRLKSFEHVKRMRYFSEIDFDTLRKLQPPFVPQLDDETDVGYFDDFGNEEHMARYGDVFKRQAKMETSSTQNDVMSNILGFTFKHRKGKSGSTGVLYDGANNVDPFATFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0L4</accession>
    <name>A0A1E5R0L4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">E3 ubiquitin-protein ligase complex SLX5-SLX8 subunit SLX5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4456</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0L4">
        <property type="interactions" value="77"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0L4"/>
    <dbReference type="InParanoid" id="A0A1E5R0L4"/>
    <dbReference type="OrthoDB" id="3972878at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033768">
        <property type="term" value="C:SUMO-targeted ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038886">
        <property type="entry name" value="E3_SLX5/Rfp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28042">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE COMPLEX SLX5-SLX8 SUBUNIT SLX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28042:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE COMPLEX SLX5-SLX8 SUBUNIT SLX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="517"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="772" mass="87184" checksum="A97CEB5BF0876225" modified="2017-01-18" version="1">MTNLRNQHEEETRPIDTSSIARQGDPAYSEQTGGDDTSRNSSQSVVEISDAEEDTTAEADTQSSGYLSSKASMRRSRIQQRLEERLRERSNGSHTPNTVSRPKRGQRPQRDRVGLFTNAEGLNRLSDTSNEVEIVREIVFQHNVNHTENREFDPSLITADNREVIDLEVNDNSLGDVAEVPQRNQSQQATGGDVQQESDVENEDMEILQQTNTINTNPLPADVMTDSNYIQIQLPGNNNMYVYDRNGSMQRDRASFESTMLRNYERERSQESTQQRHLRRLNRGAERQRNQLMHVSRRATDSILLGENEGLFLVDDDQNLQSVNTAEPSRFQPITAANSQVVAANGPSVRNSNATDVPMSVRRLRQQLATQRRMQVMQQRRAQQMLQRASGHADLEVNTELTDVREKIAKYPLAIKKAFDDCETLFTFQQTVNRTMPTGATEAIKNLKNLFIRYRSIRGNSRLXQYTQAVETTSSAVVASRVSEPHYRRGHHRNGYEEESATREAGRDYMRFRRNMGLGQSGNDAMRGPTGESHRGDDDTYRRAYLESGYFAPMLGNEEETTRSIIQMIERREAAERSALTNKFSESTEAQKNKIYGKAGNLPSGYTSSFSSDILMNRDKMQNTNVSNGNTSSVVAEVMEGYEKVPICVLCGVELGXGIPLDFEGKRSIDQSFEYMVEKYGVACPFQALTRPTDYDRECSKRCFVAKPCGHVFCGRCVLRVNHATRLSAGAKKSMSRFKGPSNPNNYGPNTCVGENCHVKLRKKGAFQEVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0N1</accession>
    <name>A0A1E5R0N1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Maintenance of telomere capping protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4541</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0N1"/>
    <dbReference type="FunCoup" id="A0A1E5R0N1">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R0N1"/>
    <dbReference type="OrthoDB" id="3972991at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="706"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="400"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="442"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="494"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="509"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="739" mass="84420" checksum="AD2F619445E30594" modified="2017-01-18" version="1">MGFHKSGTVVKLQGLNDLSAQDPRNNNSHKFAHDFLNIHSASTLAPRLHSHTVQSPDSTSASSYEDEYKSAHRVNQSSASSSSPSTSLQNKREGTEENELVVRPRRNNFMNGSAMHNDFTEEIAPSAGDDDNSEKAAAKSDKALLNAKSLTLWLVKNTKPQLLLDFNIDESSNAKKDYTNLSESAGLGSSLITDPKLRVQIVKVRNYLAHYYMLLDSTNGTYNPLKTMRNRKLQHQGMRANHRDTFRKTHAVTDPPAIPVLVFSRRNRPKDEINELGIDAVNENWEKKRAKKHYSEHEHRHYKESRTSRSHNPIHRRKGKNKGKYVFWKWFVGVNERYDDINWERVQVLRSQMNSSENIDDTEQPSPEILEDNEANFAHDAFQQYEEKVPADVEHVEDAGTSKDTLEQTEGNDKELSHGNTPPQVIVTDEKDHQQNLQRVLTGQNNNHEPLGATSETPMYSLSGTPRASQSALSNKTYSPNLLDIKLRPKHTRQGTSSSISSDINVDGSLSLTKSVSSSMDSEKEYLSSTKKKSQSPPALLEHVIIESNQSTIGQKCKEQSSFQHMKKLQYQEFQYFKSIQKILMVQHANMRLSYEKHIAKFDSYEYKKNQEKELAGDEKALRELRELEMIGTDKYSNYLPPSNDIEELIFTNDRILTEINTSLTLRLKMCGNATARYADSEDENGQSKIHKMRMFNKLCKSKDKIFYVIIEYVITLSFRMIWLVFVIFKGFLKVLGFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0Q4</accession>
    <name>A0A1E5R0Q4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Telomere length regulation protein TEL2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4625</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TEL2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0Q4">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0Q4"/>
    <dbReference type="InParanoid" id="A0A1E5R0Q4"/>
    <dbReference type="OrthoDB" id="10258062at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051083">
        <property type="term" value="P:'de novo' cotranslational protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.720">
        <property type="entry name" value="Telomere length regulation protein 2, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038528">
        <property type="entry name" value="TEL2_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051970">
        <property type="entry name" value="TEL2_Regulation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019337">
        <property type="entry name" value="Telomere_length_regulation_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15830">
        <property type="entry name" value="TELOMERE LENGTH REGULATION PROTEIN TEL2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15830:SF10">
        <property type="entry name" value="TELOMERE LENGTH REGULATION PROTEIN TEL2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10193">
        <property type="entry name" value="Telomere_reg-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Telomere length regulation protein conserved" evidence="2">
        <location>
            <begin position="430"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="682" mass="77610" checksum="1D7F43AC222F3E44" modified="2017-01-18" version="1">MDNAPEKWIKRLKDNPSSXDFLDAVDALCAKPEDKIHIATFPAIVQLVLPLTASLPQNTLDNIDAVVANHVECLSQLVIGSDISTLNEVCQKTVFRLLWKHRSCLNTYLHQENLSKAQISIIKALFFGSKIYNFINGHTSIEEYSELVLQQLQNLQNSNARKKQYGDLLLAFLKLNPIVVXQVFFSSWIFDSTQAFENNYLDKFYQNSITINKKKLNHILLKYVGKHVTHENIGSISYIMKKLNPLFIDYAWITYSCSSEIIQYVYIVSLPTRWLLRYLNYLFVEWGTSNIDGKRMDEEDITLSQLLQLAITQLTTIDKQKLPSSSSFMDAITQRLHNHNKNYINRTMCIAKSVVGDTLKYDVPCCLEVPYYKVSTALEGSSLLDLILNDNILVNSCSTMEKTLHSLELKDADSDDEDEHTESVTARKIIFLKDLIEQFTKLQQSNADTYSTMQVSFKLIMDKGGNVSELQYYCDEMINVLLKLSNEHEHKDFEQLRVRNVTAILNLLPRKITTVLELLLTQDLTLQQRLSILTVVGLSAQVLSGLQEVGPEASQIENPLDXLESNKTSNTLDRGSVVWKSKKLGDDNGLDTLHSVXQKTKVNRFNAVAPLFFWPLVNGWLAGIDLGTFDFLFKSHYVNTLAMIFSLSRAHNDIVNMEESMVLVLQQASDQNIKLQSGDIFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R148</accession>
    <name>A0A1E5R148_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R148"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="69" mass="8242" checksum="C241032EEC80CAE6" modified="2017-01-18" version="1">MTVGVIYNYETENKGDTSKNKKNNVKXRQRDDTTTRQRDDTTTRQRDDTTTRQRDDTTTRQRDDTTTRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R197</accession>
    <name>A0A1E5R197_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4264</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R197"/>
    <dbReference type="FunCoup" id="A0A1E5R197">
        <property type="interactions" value="145"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R197"/>
    <dbReference type="OrthoDB" id="306876at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="305"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="328"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="37"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="212"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="352"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="355"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="397" mass="44118" checksum="9C44B4D404FECB06" modified="2017-01-18" version="1">MHETNHNNKENTQSFDEEDFHIDDESGLRNSGVSHAGIYYLLASQLFSSVPVILTKLLVADPVFQDDPLKPFEILFPTMFLTLCCTLLYVYIKRNSIPFAPFGLPEVRKWLVARGSFGFFGVFGLYASLKALSVSDAVVITFLTPAVTGILAKVFLKEQFSKVEGVCGAIALFGVILVVRPTFLFHNDENDISEPTDLVESSSPKERLTATVFGLIGVLGGSSIYIIIKHIGSRAHALINVTYYAFFTCFISLLGILLSSKTRLRLPTTFNHWLIYVAIGLSNFGSQVTFTVGIQKEKKTSRSALVIYSQILYALIWDVLIFHHVPSFWSWCGIIVIIASTVYALFYKSTHDQSPKSSSSDSEFGSPSPLPDRDLDNGSIEFELDDIARDPMVVDHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1E6</accession>
    <name>A0A1E5R1E6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Sporulation protein RMD6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g4032</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1E6">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R1E6"/>
    <dbReference type="OrthoDB" id="4062597at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ80716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="222" mass="25042" checksum="DAD6FE95FB108275" modified="2017-01-18" version="1">MAESGSLVVLTGTDLQKCDKSIILALFNVINXAYHRNNVKFNIMKNDRIKDVSLFLSDIGFDKNADNCVLIVYFEGPVANAHAFDVGVFEGIQVYTPKRSVKSIPPFSANNNILGTVGFKPHHEYADGEHFEVTSFTSLKPKLGTVLLPLCENYFATLFKVKQYIAEVVVEHDLVPYYTEKHNYVETKRFFVPKDYDPVTTGLEPGIEAEPFFMATMIKDFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1E9</accession>
    <name>A0A1E5R1E9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Restriction of telomere capping protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4077</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1E9"/>
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    </dbReference>
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    <dbReference type="OrthoDB" id="2567806at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1250.10">
        <property type="entry name" value="Ribosome maturation protein SBDS, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036786">
        <property type="entry name" value="Ribosome_mat_SBDS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019783">
        <property type="entry name" value="SDO1/SBDS_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01172">
        <property type="entry name" value="SBDS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89895">
        <property type="entry name" value="FYSH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS N-terminal" evidence="1">
        <location>
            <begin position="11"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="117" mass="13070" checksum="EA008ADDEC0D6F4E" modified="2017-01-18" version="1">MPEHPQNPQQAVKVFYKGESTDFLVFGTNKAAVEQYRAEPNINNFLEATTLHQVFVNNTGRGKEGNLGEASKTQLENEFGKGKKIEDFIQTILKEGEISDSKDIGGHKYSSTNDSKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1V0</accession>
    <name>A0A1E5R1V0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Signal recognition particle receptor subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g3899</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SRP receptor beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1V0">
        <property type="interactions" value="618"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1V0"/>
    <dbReference type="InParanoid" id="A0A1E5R1V0"/>
    <dbReference type="OrthoDB" id="41266at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019009">
        <property type="entry name" value="SRP_receptor_beta_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09439">
        <property type="entry name" value="SRPRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="218"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="221"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ80885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="300" mass="32771" checksum="E982624C1FB6F89B" modified="2017-01-18" version="1">MQLGLFGTVGILLVVLFVSALVLFKRGQNAGLRNGVSVGGSNSKSNNGKPTFLIVGPCNSGKTLLTYTLRFLAANEEVSGTETTHNTQELDVPLTVTSQAPLELINYXLPTLGANNKKFNLIDFPGHVKVRYQLENYIKGNKNLKGVIFVMDSTDNGEKLVDTVXFLFEVLNYVEKYHNGVDILIACNKKDLFAARPANKVEEQLQIKLKELTVRKRKSVATKTSSHGSTGGPASSGKSRGGRQLVAASTNSDDSQELYAEHLMSSAFTFQDLDGSFDSISGSVLRNEITNWIEWIGERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R291</accession>
    <name>A0A1E5R291_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Suppressor of mar1-1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4013</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R291">
        <property type="interactions" value="760"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R291"/>
    <dbReference type="InParanoid" id="A0A1E5R291"/>
    <dbReference type="OrthoDB" id="3971571at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="488"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="301"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="364"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="436"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="506"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="535"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="574"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="625"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1003" mass="113908" checksum="C4540EE7D1745891" modified="2017-01-18" version="1">MTENTRIDGNDSNTVISQEVQNMKTQINQFADLVQVLTKNDKSSGDSLHLLKENVKKLIYNQQVLENKLDDCLKNQFNTDQVVNDLNWKLSRLLQESSAMKRSFHAEEANMSNGSRKRVKMNGVTVKPKAKGSKHFDEIMDTKCTANNGYGVSPHSNHNQADSNDDNVSGAAASIVLLPNGQMQIPKSKRHFIDPTKNGKLLVSEHSLKKSSTATEDGKARAMKNSEPKTELNKSLDDTNKNLRRSSRRSAQNSVNATTNKGTEQEADERSNDLQTNSGIAIVNEMENGKKELDQSQAGEEVLEEEEEEAEEADKHDLEAIKANTQEDQVGTHENDSFLSIKNSRLNVASKDEEDASESENEGQDIHGEKDKGGDQEKVEKDEKEPPLNKMQFGHDLHQSAKEMMELNRAKLQAIRNEEKNNRALKRNNQETLTRSSSISSSSSSSSNLSSSSKISLATTNTTEVSKTKDFSIKPAMKFKEMTPHSALIQNSVDIPSNKDMPLIDVNEEDNSESDADEDQEHENTKMGKAIGFLDRPEQDNDTKKVNKDGKAIELKVETDNDKQFRSRLGTGIENDKESDTSNKPVNSDHSEVKNLEKETTKEPGLSAHSKTDHNESNENFSSSDEFKDDSVYEKTESPPLQDFSIGDSWSASGLAQAFSKSSXSKQNSLLTGQLYYTGKPTLSDVEANVISQSNPLFHHQNEVDKLTGDLPLTLNEAVNALSEKQREKLASQRSSALSASTFLSFHVELKMVKGVYYRVDTGKVLTRDAFMEMKYRTSFYPKWNSSMDVPSPITMSKLMQVSQIVNGYRFYALSNATMQNINQATRNHKGDKLNEYMLDGTLEEELEETYEKLGQFPLTESFFNTLPYFLMEFRWENLLISRKFDVAESKRTWQRRKALFALLHAWREKTIEERTLYFGKDNNTFTLYHCVRELEAYRILLNKSVSWFYNHITMLRIILFELFYHHGNNLHKELQEKDLPKYKTGPDEFWREWMFPRHANLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2F7</accession>
    <name>A0A1E5R2F7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">arginine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Arginyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4137</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Arg) + L-arginine + ATP = L-arginyl-tRNA(Arg) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2F7"/>
    <dbReference type="FunCoup" id="A0A1E5R2F7">
        <property type="interactions" value="1035"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2F7"/>
    <dbReference type="InParanoid" id="A0A1E5R2F7"/>
    <dbReference type="OrthoDB" id="68056at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07956">
        <property type="entry name" value="Anticodon_Ia_Arg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00671">
        <property type="entry name" value="ArgRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000006">
        <property type="entry name" value="Arginyl-tRNA synthetase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000058">
        <property type="entry name" value="Mitochondrial arginyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="entry name" value="Arginyl tRNA synthetase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00123">
        <property type="entry name" value="Arg_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005148">
        <property type="entry name" value="Arg-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956:SF11">
        <property type="entry name" value="ARGININE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="ARGINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03485">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM01016">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55190">
        <property type="entry name" value="Arginyl-tRNA synthetase (ArgRS), N-terminal 'additional' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arginyl tRNA synthetase N-terminal" evidence="12">
        <location>
            <begin position="36"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="DALR anticodon binding" evidence="11">
        <location>
            <begin position="501"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ81070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="621" mass="71061" checksum="645ED130AEB58377" modified="2017-01-18" version="1">MSTDILTQLNKLNLEDASASNLKVAPNSYPKENVIDFYRNYIAEQLSKITEIDSSIIFPALEWTNTLVKGDLLIPVPRILRGKKGVNPQEIAKEWSEKLNALKSPYLLETVANGTFLQFYFNAQSTLFSTLIPQILSNTTKYGSLPLIENKSKKLIIEFSSPNIAKPFHAGHLRSTIIGGFLSNLYEAMGWDVVRMNYLGDWGKQFGLLAVGFNKYGDEKKLEADPINHLFEVYVQINKDVESEKETLEESDPNSTNNQARLYFKKMEDGDKDALKMWETFRNLSIKKYINTYARLNIKYDVYSGESQVSKDVMNKALEMFEEKNLVTEDKGAKLIDLTKFNKKLGKTLVQKSDGTTLYLTRDVGEAIQRYENYHFDKMIYVIAMQQDLHTAQFFEILKQMGFEWASKLQHVNFGMVQGMSTRKGTVVFLDNILEETKDKMHEVMKKNEAKYAQIENPDEVADLVGISAVMIQDMQAKRINNYEFKWERMLSFEGDTGPYLQYAHSRLRSVERKAGDLFNVEELVNADFSLLNKDECAATLVRILAQYPDVLRNAMKTHEPSTIVTYLFKLTHQVSSCYDILWVAGQEKNVAQARLALYAAARQTLNNGMRLLGLTPVDRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2I9</accession>
    <name>A0A1E5R2I9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 2 subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3880</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2I9"/>
    <dbReference type="FunCoup" id="A0A1E5R2I9">
        <property type="interactions" value="1329"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2I9"/>
    <dbReference type="InParanoid" id="A0A1E5R2I9"/>
    <dbReference type="OrthoDB" id="1685042at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04452">
        <property type="entry name" value="S1_IF2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1130:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000015">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1130">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.190">
        <property type="entry name" value="Translation initiation factor 2, subunit 1, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044126">
        <property type="entry name" value="S1_IF2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024055">
        <property type="entry name" value="TIF2_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024054">
        <property type="entry name" value="TIF2_asu_middle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011488">
        <property type="entry name" value="TIF_2_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07541">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110993">
        <property type="entry name" value="eIF-2-alpha, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116742">
        <property type="entry name" value="eIF2alpha middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1 motif" evidence="4">
        <location>
            <begin position="17"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ81112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="302" mass="34189" checksum="B12D8414B27ADD50" modified="2017-01-18" version="1">MSTTHCRFYENKYPEVDDVVMVNVQQIAEMGAYVKLLEYDNIEGMVLLSELSRRRIRSIQKLIRVGRNEVVVVLRVDKEKGYIDLSKRKVSSEDIIKCEERYQKSKAVNVILRQCAEKYNLPLEKLYETIAWPLSRKYGHCYDAFKMSITDESILNDPEVKPTSDEVMADIKAQISRRLTPQIVKIRADVEVSCFGYEGIDAIKKALQSAEDLSTEQLSIKAKLVAAPLYVLSTQSLDKNKGIEQLEEAINKITEVITSYGGVCNVTMAPKAVTASEDAELKALLESKEQDEKSDSDDSDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4M2</accession>
    <name>A0A1E5R4M2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alcohol dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3733</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10736"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4M2">
        <property type="interactions" value="1046"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4M2"/>
    <dbReference type="InParanoid" id="A0A1E5R4M2"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000947">
        <property type="term" value="P:amino acid catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08297">
        <property type="entry name" value="CAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000039">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000002">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF3">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="10">
        <location>
            <begin position="18"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ81818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="352" mass="37261" checksum="47A085A60AF6BB4B" modified="2017-01-18" version="1">MSVEIPKTQKAVVFYKNGGKLTYEDIDVPTPKSNELLINVKYSGVCHTDLHAWKGDWPLPVKLDPYLVGGHEGAGVVVAMGDNVKGWKIGDYAGIKWLNGSCMNCEECELGNESNCPMADLSGYTHNGSFQEYATADAVQAAKIPKNCDLAEIAPILCAGITVFKALKTADLSPGQWVAXSGAAGGLGSLAVQYAKAMGLRVVGIDGGEGKKELFEKCGGEVFVDFTSEADIPAAIIKATNGGAHGVINVSVSEKAIEQSTQYVRSNGTVVLVGLPAGAVCKSSVFDQVVKSYKIAGSYVGNRADSREAIDFFSRGLVRSPIKIAGLSELPRIYDEMIAGKILGRYVVDCAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4N6</accession>
    <name>A0A1E5R4N6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphatidylglycerol/phosphatidylinositol transfer protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3739</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the intermembrane transfer of phosphatidylglycerol and phosphatidylinositol.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NPC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4N6">
        <property type="interactions" value="115"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R4N6"/>
    <dbReference type="OrthoDB" id="3970294at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00917">
        <property type="entry name" value="PG-PI_TP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.220.10">
        <property type="entry name" value="Ganglioside GM2 activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036846">
        <property type="entry name" value="GM2-AP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003172">
        <property type="entry name" value="ML_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033917">
        <property type="entry name" value="ML_PG-PI_TP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039670">
        <property type="entry name" value="NPC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11306">
        <property type="entry name" value="NIEMANN PICK TYPE C2 PROTEIN NPC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11306:SF0">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROL_PHOSPHATIDYLINOSITOL TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02221">
        <property type="entry name" value="E1_DerP2_DerF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00737">
        <property type="entry name" value="ML"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184605" description="Phosphatidylglycerol/phosphatidylinositol transfer protein" evidence="8">
        <location>
            <begin position="24"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="MD-2-related lipid-recognition" evidence="9">
        <location>
            <begin position="58"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ81875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="186" mass="20118" checksum="D02E8DF36BE50178" modified="2017-01-18" version="1">MLNLTNTLNRLLIVLSLSVLIQSFPVSNIQALFGFNGNKDTSVISVEGDKPIXGDSPLLLCDVEEPQLIRIGSVDLSPNPPQRGTTLNITAVGSVLEEITEGAFVDVEVRLGYIKLLSQTYDLCEELEKNDIGGLKCPVAPGLYQIKKTVDIPQEVPAGKYNVIARAYTENVDELTCVTGDVIFSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4T8</accession>
    <name>A0A1E5R4T8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nuclear transport factor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3683</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4T8"/>
    <dbReference type="FunCoup" id="A0A1E5R4T8">
        <property type="interactions" value="1185"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4T8"/>
    <dbReference type="InParanoid" id="A0A1E5R4T8"/>
    <dbReference type="OrthoDB" id="6507044at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00780">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000005">
        <property type="entry name" value="Nuclear transport factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045875">
        <property type="entry name" value="NTF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12612">
        <property type="entry name" value="NUCLEAR TRANSPORT FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02136">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTF2" evidence="4">
        <location>
            <begin position="52"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00026247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ81904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="169" mass="19330" checksum="CAA37F0652E6F78C" modified="2017-01-18" version="1">MVISVQVEIQLNSQVFPTSTSNKHFQQEKQNSPPLPSTFPYFPTMSVDFNTLAQQFTEFYYNQFDSDRTQLGNLYREQSMLTFETSQLQGAADIVEKLVSLPFAKVAHRITTLDAQPASPAGDVLVMITGDLLIDEEQNPQRFSQVFHLIPENNSYYVFNDIFRLNYSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4Y9</accession>
    <name>A0A1E5R4Y9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein CAF40</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3788</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNOT9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4Y9">
        <property type="interactions" value="886"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4Y9"/>
    <dbReference type="InParanoid" id="A0A1E5R4Y9"/>
    <dbReference type="OrthoDB" id="1183224at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000661">
        <property type="entry name" value="Cell differentiation family, Rcd1-like containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007216">
        <property type="entry name" value="CNOT9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12262">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04078">
        <property type="entry name" value="Rcd1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="288"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="288"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ81941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="382" mass="42538" checksum="4D47048686FD90A8" modified="2017-01-18" version="1">MNEVNXKFNIFSVNKTNSNFKRQIEMNKTATASPMNMNNNNNNNNNNNSPNPTKTGSSTTGTRALDDPNVYHWICQLTYGPQREQALLELGRKREQYDDLAIVLWNSFGVMTSLLQEIISIYPFLNPPNLLTNQLSNRVCNALVLLQCVASHKDSKFQFIRSQLPFYLFPFLSTTNKSRSFEYLRLTSLGVIGALVKNDSKEVVQFLLKTDIIPLCLRIMESSTELSKTVAIFILQKIIMDDNGLQYICSTQERFMAVSNVLNHMVEHILNTPTGAAATAIGVSNATGNAPGTATNNTPAGQDTPSTTPPARLLKHIVRCYLRLSDNLEARQLLKHVLPRSFVENTTVVGNVNGNGVSIAEILNDDVNTNKCLNQLLNTLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R549</accession>
    <name>A0A1E5R549_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Coatomer subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g3789</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Oligomeric complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Golgi apparatus membrane</location>
            <topology evidence="1 11">Peripheral membrane protein</topology>
            <orientation evidence="1 11">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasmic vesicle</location>
            <location evidence="11">COPI-coated vesicle membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the COPG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R549"/>
    <dbReference type="FunCoup" id="A0A1E5R549">
        <property type="interactions" value="1278"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R549"/>
    <dbReference type="InParanoid" id="A0A1E5R549"/>
    <dbReference type="OrthoDB" id="1074925at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1480">
        <property type="entry name" value="Coatomer, gamma subunit, appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017106">
        <property type="entry name" value="Coatomer_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013040">
        <property type="entry name" value="Coatomer_gsu_app_Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037067">
        <property type="entry name" value="Coatomer_gsu_app_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10261">
        <property type="entry name" value="COATOMER SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10261:SF0">
        <property type="entry name" value="COATOMER SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08752">
        <property type="entry name" value="COP-gamma_platf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037093">
        <property type="entry name" value="Coatomer_gamma_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 11" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="13">
        <location>
            <begin position="14"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer gamma subunit appendage Ig-like subdomain" evidence="14">
        <location>
            <begin position="665"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="621"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR037093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ82021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="770" mass="86585" checksum="D400F8059E947922" modified="2017-01-18" version="1">MSAKTYKARAEGDQMTVYQDCLTTFNESPINPKKCRKLLTRLYKILITKGLTTFNTMECTSLFFSISKLFQHQNNDSLRQMVYLVIKELSSVSEDTLMATASIMKDVQHVDSIIKPNAVRTLTRVLDSSTAFSAERLLKSCTISKDPSIASASIVSSYHLLPIADQTIKRLSNETMENIEALKSFVNYQPSGCEVPVFYPQSKYMSQYHALGVTYQLKKNDKIGLMKLVHQYDPSVLKNQFALVEWCKLIGHLIERDPSLIEENGHYSQNGAKPSSDNENEYIKFLRWGCYKKIEAVQLEVCKIVLKLRIVKLVDLVFQTLSSFLSVPRTSTRFAAIKILNQYAAVYPGNLKASINGDLETLINDSNKNISTYAITTLLRSGDASNIQSLINNIQINDVSDDFKIIIIDSLKSLVSKFPETISKFLVDLLKNNDGSVIFKTKIVDCLVDVLQSEENLGTTKTEILENLCDFIEDCEYTEVLVRILYVLGEYGPKLDFENSSLYVRHIYNRVNLENSIVRSAAVIALSKFPVFTKNLLETIKANDIDDEVRDRAAIVLSRAAQTSTPTASSLSIKKKSHQQLSRDIRVLESKLNIYIQNGDFSTFELPAISEPVDSGNADVDMVSGGLEGSAHGSHEPSSNSATTNDEEYKEIIDTIYHKYGGASVLGKLVSISPLQELTDSSSEFIIYAQKYLFQQDSSEKTIIFNFKVENTLDFAVQNLKIGSSDDVQISNLEPNERTDVFLSEQELQDEYVLRFIADGFEEIFEFNSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R569</accession>
    <name>A0A1E5R569_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Histone chaperone</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3715</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with histone H3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ASF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R569"/>
    <dbReference type="FunCoup" id="A0A1E5R569">
        <property type="interactions" value="862"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R569"/>
    <dbReference type="InParanoid" id="A0A1E5R569"/>
    <dbReference type="OrthoDB" id="29755at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006337">
        <property type="term" value="P:nucleosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1490:FF:000001">
        <property type="entry name" value="Histone chaperone ASF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1490">
        <property type="entry name" value="Histone chaperone ASF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006818">
        <property type="entry name" value="ASF1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036747">
        <property type="entry name" value="ASF1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017282">
        <property type="entry name" value="Hist_deposition_Asf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12040">
        <property type="entry name" value="ANTI-SILENCING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12040:SF0">
        <property type="entry name" value="HISTONE CHAPERONE ASF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04729">
        <property type="entry name" value="ASF1_hist_chap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037759">
        <property type="entry name" value="Histone_Asf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101546">
        <property type="entry name" value="ASF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="185"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="192"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="268"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR037759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ82041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="299" mass="34919" checksum="2AF2D0CB322FEEFC" modified="2017-01-18" version="1">MSVISLLNIDVLNNPSKFTDPYEFEITFECLDHLAKDLEWKLTYVGSSKSLEHDQVLDSILVGPIPVGINKFKFVADPPSAELIPNNELVSVTVILLSCSYDNREFVRVGYYVNNEYDNEEMKLEPPKKVDVQHVVRNILAEKPRVTRFNIVWDNKDEDNDNEDQVALDKEINKRFEQENKRDLKKRKLNPDEDEEENDDEEEEEEEEEEEEEEEEEEEEEEEDDEEDEDIDESEDIEDSEGEEDAFDEQKELQAEEQGQAKQLQEQEREEADEELAEQNIDAQDTHNGDDDLVATPKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5B6</accession>
    <name>A0A1E5R5B6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">RING-box protein HRT1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g3817</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RING-box family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R5B6"/>
    <dbReference type="FunCoup" id="A0A1E5R5B6">
        <property type="interactions" value="1032"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5B6"/>
    <dbReference type="InParanoid" id="A0A1E5R5B6"/>
    <dbReference type="OrthoDB" id="8962942at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000273">
        <property type="entry name" value="E3 ubiquitin-protein ligase RBX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051031">
        <property type="entry name" value="RING-box_E3_Ubiquitin_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024766">
        <property type="entry name" value="Znf_RING_H2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11210">
        <property type="entry name" value="RING BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12678">
        <property type="entry name" value="zf-rbx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="60"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ82091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="126" mass="14776" checksum="A772EE5359A7607F" modified="2017-01-18" version="1">MSEDQHMDIENETQSHTTDHSHSHEPVTTDQQQQEGEKRFTIKKWTAVGFWSWDMEVEDCPICRNHLMEPCISCQQNSMTDAQVECPAAWGVCNHGFHLHCINQWLTTRNVCPLDNQVWKFKKYGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5D6</accession>
    <name>A0A1E5R5D6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA pseudouridine(32) synthase</fullName>
            <ecNumber evidence="3">5.4.99.28</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3808</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>uridine(32) in tRNA = pseudouridine(32) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42544"/>
            <dbReference type="Rhea" id="RHEA-COMP:10107"/>
            <dbReference type="Rhea" id="RHEA-COMP:10108"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.28"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.28" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R5D6"/>
    <dbReference type="FunCoup" id="A0A1E5R5D6">
        <property type="interactions" value="497"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5D6"/>
    <dbReference type="InParanoid" id="A0A1E5R5D6"/>
    <dbReference type="OrthoDB" id="424794at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019239">
        <property type="term" value="F:deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016814">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160151">
        <property type="term" value="F:tRNA pseudouridine(32) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02557">
        <property type="entry name" value="PseudoU_synth_ScRIB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000061">
        <property type="entry name" value="DRAP deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.10:FF:000017">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006225">
        <property type="entry name" value="PsdUridine_synth_RluC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050188">
        <property type="entry name" value="RluA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00005">
        <property type="entry name" value="rluA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600">
        <property type="entry name" value="MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600:SF40">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE RPUSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18785">
        <property type="entry name" value="Inv-AAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="7">
        <location>
            <begin position="436"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00036184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606225-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ82114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="589" mass="66573" checksum="E24E9AC122231238" modified="2017-01-18" version="1">MSEFEKQNVQDHEKDCIITDPNTVEKDTAHPVNSGSKLQNTNMKMNKKQKTSKSLKVTDQAGFRVRVQESRQKDPEYEVEIDGPLRKIKPYFFEYNTFCKLRWRDRNLYDVFITEFRDKSKEAYQKTIEDGDVLVNGQVADMNTIVRNGDCITHRAHKHEPPITSKEIKIVFEDENVMVIDKPSGIPAHPVGRYRFNSVSKLLFRQLGYPAHPSNRLDRLTSGLMFLAKNPTGAEEMRTQLVQREVSKEYVARVVGEFPVGEITVDIPLKSIEPRLGLCGICHDHDDPEGKNAKTVFRRISYDGETSIVRCKPYTGRSHQIRVHLQYLGHPIANDPMYSSLEAWGPTMGKNGDANFAQVCEILDQVGKTKSASSWFHPHSHGEVLKDEKCEICGSDIYTDPGPNDLSLWLHSYKYESTDPARPWTYKTEFPDWALETHKKYMELAIKEAEKCEPTTTAFGVGAVLVSGNTILATGYSRELEGNTHAEQNCFAKLTAAETDIPEGSCLYTTMEPCSTRLSGNKPCLDRILEYGQKITTVFVGVMEPATFVKNNVSLEKLQQNDIDYILIPGYEQQCIAAAEKGHETKVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5F1</accession>
    <name>A0A1E5R5F1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Bypass of stop codon protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3830</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5F1">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5F1"/>
    <dbReference type="InParanoid" id="A0A1E5R5F1"/>
    <dbReference type="OrthoDB" id="413079at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000286">
        <property type="entry name" value="MFS efflux transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051788">
        <property type="entry name" value="MFS_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23514">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23514:SF3">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="314"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="344"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="383"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="407"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="447"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="474"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="93"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ82137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="510" mass="57744" checksum="E445EF2319AD5FDD" modified="2017-01-18" version="1">MTTDRLESPLGFEENDSKDLNYSVDARDXANGFDSPTLLNNPHQKEEMSFDDPMQDPLNSLAPNTYSKVFDRNGHEITLYPLNYQTHRVVVYQIISCLVIFTILGLNDQSTGSLIPVLTEHYNVSHVQVSNIFLVQTFGYATSSLMNDKLHRMFGQRGVLILGSLFLGLCTFCLSLKVKWFSLYVLFYYPIGVSIGLVDSVVNVFFGSLQIHKNELLGLVHSIYGCFSFITPIIVNRCGKEHWNRFFYIPCVLGLTGALLSIPSFRLETKEKYIYLCDEKDVVENQESDQLATTSEETNSSIFKLFKEQPVVPLYAIYLFFYLGTEVGTGSWIFTYLLEVKQGAARGMAYITASYWAGLTFGRLYFGIMTKKWFPNEYQASRFYGNVTFLFNLMFVLFGFIPQNSNFYFVLLSCCVFMAGMWIGPLFPLASVVALDVLPKRLHISGVSAAISLGSTGGAILPWIFGVIMQQIGIKAFPCLVLGTNAIFQLVWLLYPKFVKNKQNVFYDRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5M8</accession>
    <name>A0A1E5R5M8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3668</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R5M8"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ81843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="81" mass="9488" checksum="E54B751B6A5C236C" modified="2017-01-18" version="1">MKLXFTAXFTDKRAEYSGDTQKEAMVKTWKVKTRVCIVILKNLEQVHFGRSGRAKQXHNKVNKYVFPDVDEDDKEYSRDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R6H4</accession>
    <name>A0A1E5R6H4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">6,7-dimethyl-8-ribityllumazine synthase</fullName>
            <shortName evidence="9">DMRL synthase</shortName>
            <ecNumber evidence="4 9">2.5.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3835</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 9">
            <text>(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:26152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="2.5.1.78"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homopentamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the DMRL synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.78" evidence="4 9"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R6H4"/>
    <dbReference type="FunCoup" id="A0A1E5R6H4">
        <property type="interactions" value="305"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R6H4"/>
    <dbReference type="InParanoid" id="A0A1E5R6H4"/>
    <dbReference type="OrthoDB" id="2965at2759"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00404"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009349">
        <property type="term" value="C:riboflavin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000906">
        <property type="term" value="F:6,7-dimethyl-8-ribityllumazine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd09209">
        <property type="entry name" value="Lumazine_synthase-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.960:FF:000007">
        <property type="entry name" value="6,7-dimethyl-8-ribityllumazine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.960">
        <property type="entry name" value="Lumazine/riboflavin synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00178">
        <property type="entry name" value="Lumazine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034964">
        <property type="entry name" value="LS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002180">
        <property type="entry name" value="LS/RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036467">
        <property type="entry name" value="LS/RS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00114">
        <property type="entry name" value="lumazine-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058:SF0">
        <property type="entry name" value="6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058">
        <property type="entry name" value="6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE DMRL SYNTHASE LUMAZINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00885">
        <property type="entry name" value="DMRL_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52121">
        <property type="entry name" value="Lumazine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ82143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="164" mass="18090" checksum="B827D49D60B08F33" modified="2017-01-18" version="1">MAVKGLGQIDQQYDGSKIKVGIIHARWNRVIIDALVDGAIKRMKELGVTEENIIVETVPGSYELPYASEVFMKKLHPEIDVVIPIGVLIKGSTMHFEYISESVTHALMNLQNKVKKPIIFGLLTCLTEEQAMARAGIDEGKTMHNHGEDWGAAAVEMAVKYGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7G3</accession>
    <name>A0A1E5R7G3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative cytidine deaminase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3157</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7G3">
        <property type="interactions" value="169"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7G3"/>
    <dbReference type="InParanoid" id="A0A1E5R7G3"/>
    <dbReference type="OrthoDB" id="414540at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004126">
        <property type="term" value="F:cytidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055086">
        <property type="term" value="P:nucleobase-containing small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072527">
        <property type="term" value="P:pyrimidine-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01283">
        <property type="entry name" value="cytidine_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050202">
        <property type="entry name" value="Cyt/Deoxycyt_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11644">
        <property type="entry name" value="CYTIDINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11644:SF2">
        <property type="entry name" value="CYTIDINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="2">
        <location>
            <begin position="50"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="229" mass="25842" checksum="C508A9614FDFDFB5" modified="2017-01-18" version="1">MTRLVMRYTLTNHSNXLFGNQELDQEAKKNSPYHSETNIENXIDTKLCPNXINTLKQKTVXAQTFAYAPYSRFKVGCCLLVQKPRPKHXKDYXNSTNANYKDQEYDFITGCNVENAAFPACVCAERTAICKAISMGYTKFEAICVVGGTVLQDVETTQSHNSDQHPTTTTQESFVTPCGVCRQFIREFVPKDFPVYLLSNNNCTGKDRDHKVMTIDELLPHSFGPGDLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7M0</accession>
    <name>A0A1E5R7M0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">SWI/SNF chromatin-remodeling complex subunit SWI1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3227</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7M0">
        <property type="interactions" value="346"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7M0"/>
    <dbReference type="InParanoid" id="A0A1E5R7M0"/>
    <dbReference type="OrthoDB" id="1938591at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051232">
        <property type="entry name" value="ARID/SWI1_ChromRemod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13964:SF27">
        <property type="entry name" value="HAT-TRICK, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13964">
        <property type="entry name" value="RBP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ARID" evidence="5">
        <location>
            <begin position="440"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="78"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="208"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="309"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="630"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="737"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="227"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="250"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="330"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="350"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="422"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="664"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ82909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1448" mass="162773" checksum="4B95E33B513A5A80" modified="2017-01-18" version="1">MNGDEDSLYFGGDINDEGGMNDDMFGFFDPQPLEAGNNEIPISLLDDFDEQQQQPNFNAXVSFDNSENSIAVPQANIASTSEPNNQPDFNGASEKSGIPTTNGVSNLRQLSNKTPSVTATPTYSYTNSPNVLTPQAILARSNGTAMASNSNLSNTNANTIDYSNNGNNMNSQAYRTMSPETFSASDNNAINTSSNSHPATMNNSFNSHMMNNNQTGIVNSAYPYPNNPVSRQKSPPNNYGNRAAMLNAMQQQQPQQQQRQQQQHHHHQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQHTQPNENNLAFGPGKGKQTKAKPAATRKPRQSKKATKSTTSTPTTASLASSASKNMSSNTGLNQMANNNMNQQQMPKQQAMQPQGQQASQQVPTSSSNTVSATTAGTSMNPVLDQQTQRQITSEITNKQSELFMKSLLELCKRRNVKLTRNPELNGKNINLFVLYMLVQKLGGGEKVNQTPQNWLAIGAKLNLDMMSNAPANVRQLATIYFQLLLPYEKYLISSEGMRETQANRVVLQQYLQEVIARAKAVQQQKLTQQQQQQKQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQLLQRQLFLQQQQQLQQQQQQQQQQQQAPPAQMNLQTGKPAPKKRGRKPKAETNAAKQQSPAVANFTGNLQQPPIQQPPLPLNFAPASGNMPNVNGSMPFANSFSPSQANMPNSMPMYQPQTVPPAVAQTQLAASPSVPVASEMKTGPEKKTRKKRKSKKELEAEKKNQEALLFKHKELVEAEFTKQKAILLKNREAAIAKLPKIYKKTFTRNYVPYQRDLDKPNGLSMTTIANIGDQLDSVKPSFLYLPEIGTLNLHAVTMGLRSGSLSHTNTFLNSLLLVSADSSIDIITTQELLDALCILGCQIIDKLITGDTSRKLFTKGYLNMDTKATEDDVNLLYSTMNGNNGTTENNAQIKVDTLTGEDIAQVPENETADAALSSTAIASPEVSSTPALQNEALFESWNYLPSSARDDQLPTPSAEGPGSGNFSNGLFFSYLELLRISKDDIDQPFTKMYVRAAEDPVMMYCNQLSTISMILRNSSFSESNIRKMNDNTHFKRYVFDLLYLMFVHQDKIPLTTKNLGFRKDCILGFANMSHLWRLGSLLDTLLIMFLFLSVSIVDENSSRLLPNDVTNQTFQVFAIDSFAKLSSLGNPNRNFLQTIIFNRLGDADINTNDNELAKCKALMDQFCGDDKYKMVKQLAHSLVMSLPYDFSVYRSNYVTFMKSLPVFSQAVTSLWVLCKFIENIPDIDLNLVFNLLVDCNVGSRVSLFLRGFIKFIHAYQTSPSFDSEAYEYSTRVIQNALLKSFEILTLLIKKVTLDLPIKSQDEFKTMEDYKFFKKNRTEHNGLVQKFKKELLLTTFVLPSERQLLKYISDPVLEGSVHKEAYLLHNLKKNFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7P0</accession>
    <name>A0A1E5R7P0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3142</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7P0"/>
    <dbReference type="InParanoid" id="A0A1E5R7P0"/>
    <dbReference type="OrthoDB" id="5582218at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="321" mass="37198" checksum="3F0AD484D1D3D6F1" modified="2017-01-18" version="1">MENINFTKPNLTRTSSGTDAQRSLSPLLNNQSTRKNVTATTSNITAKGLNIKHEKTTNASAMLFSNSKSLFNGIPDPPKPTSSMGSEAKHPILESNQDIEPTPQLEHWAQYPKDSPQYLLSLVQSVLPHFLIDIYTDPDDPVMLCKLSFYSLVALFIKNFVLNWYVDKIQSLNNDFLEYLDKRYISQIYEFFFTKNFCKLDFMKFLLDDIPFIVENTMLMQANDPDYEYTLKFVKVYTKQIKSPLEKAFVKSLLQDFLFGKVLKNICEPQVLLPMILMTATKKKDNHKKKTIKSAIKPQMKKHSLFSKYQNQLRNKVMNFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7Q3</accession>
    <name>A0A1E5R7Q3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histone deacetylase HOS3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3353</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7Q3">
        <property type="interactions" value="180"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7Q3"/>
    <dbReference type="InParanoid" id="A0A1E5R7Q3"/>
    <dbReference type="OrthoDB" id="5232919at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004407">
        <property type="term" value="F:histone deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053244">
        <property type="entry name" value="HDAC_HD_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47558">
        <property type="entry name" value="HISTONE DEACETYLASE HOS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47558:SF1">
        <property type="entry name" value="HISTONE DEACETYLASE HOS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="2">
        <location>
            <begin position="111"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="635"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="796"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="195"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="234"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="643"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="655"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="670"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="801"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="812"/>
            <end position="828"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="828" mass="94636" checksum="17B964735439EB47" modified="2017-01-18" version="1">MLDPLLNFKQEFQVFIDSNPHLHNALLSNSIPEHNKAQIIISPGSLFHKFERNWVSKSYKKTIVERPERLLACSIGISAALTMYPSLFTIKSIPPIGISDDHTLEVAKQKNEMYNIAKLMSPHVVKVHGKEWPGKLIKYCQQSAQHLAKGKVEVPDGWNSGDIYLSPTTLGAIANSITALETGVDTIFENKAKHSXMNRSSSHPPSSKMSTSSTAQTSLSLDDQVNEQKHHHQQQQQQQQQQQQQSPSRCFVMVRPPGHHCHIDFPEGFCLMNNVYIAAQYASDYHGVTHVAILDYDLHHGDGTQDVCFKRALSQNKEIQDLEKQREAQGSSNGNIKHEIIDEEEYFQKDYLINKYGLKVGYFSMHDINSFPTEVGYSTEDNIARASTCIMDSQNLNVWNVHMKQWNMQQNLGNNENNDGEGAANNNEEEXFYKLYNSRYRQLFSKADEFLYEGKQKMQELGKPFKGLIIISSGFDASEYETPSMQRHKVNVPTSFYNIFTKDALKLAQLHTEGKILSVLEGGYSDGALVSGMFSHLIGLQNQDWIKEWGSKQIVKELCKGCKHNWKPHKKYSSGSKSNSKDMVKLWSEQVIKLGRSMIPEFQTEFFDKCLVNNMGLFNDPKQLEQVLKELPASHTRSKTKQLKQQGKQQTITEDLTKSWEEPKLERSRVSQAIPSSKQTIMSQEYSDEDNEEEDEDYVYDEELNKTFNRTVEDITIDDISRHLETLEIIEPNTHYSDNNFSLQNNPYARRSNKYPTRVTRASAARRSKPVPNMFLTEQDENNDYSLMDITMMSAHPVRESSSTSGKTQSSRGDPTQTYRSTRSGRQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7Y2</accession>
    <name>A0A1E5R7Y2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Zinc finger protein LEE1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3261</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7Y2"/>
    <dbReference type="STRING" id="56408.A0A1E5R7Y2"/>
    <dbReference type="InParanoid" id="A0A1E5R7Y2"/>
    <dbReference type="OrthoDB" id="411372at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045072">
        <property type="entry name" value="MKRN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11224:SF10">
        <property type="entry name" value="IP09428P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11224">
        <property type="entry name" value="MAKORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="68"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="98"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="68"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="98"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ83016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="230" mass="25538" checksum="80ABB36B4B1D31A5" modified="2017-01-18" version="1">MTQGTFYTNTASFPPLTPSTPLGNTPTNLSPQFTNDSYPLDSRNFSPEQKQAIIHHLMITKSSIPVKLYPHVPCKFYQQGACQAGANCPFSHNLNTCRERPVCKYFQRGHCKFGAKCCNVHPTNNGSGASDSLPLSPISPTFHNHPTTTDIDYTYSNATNNSNFYNDPSSITGEGNNNNANKFPTTDMQDPFPFDMDEGLEYIPGELSELLTPLQIAHRRRSSERGPHAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R800</accession>
    <name>A0A1E5R800_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cyclin-dependent kinase 1</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g3265</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R800">
        <property type="interactions" value="1031"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R800"/>
    <dbReference type="InParanoid" id="A0A1E5R800"/>
    <dbReference type="OrthoDB" id="1732493at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009891">
        <property type="term" value="P:positive regulation of biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010389">
        <property type="term" value="P:regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07835">
        <property type="entry name" value="STKc_CDK1_CdkB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000375">
        <property type="entry name" value="Cell division related protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000144">
        <property type="entry name" value="Cyclin-dependent kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF254">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="7"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ83014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="294" mass="34204" checksum="8B348C47A8B55186" modified="2017-01-18" version="1">MTDLANYKRLEKVGEGTYGVVYKAIDLRHDDRIVALKKIRLESEDEGVPSTAIREISXLKELNNENIVGLYDIVYSDSHKLYLVFEFLDLDLKRYMESIPKDQTLGDKIVKRFMLQMCKGIEYCHAHRILHRDLKPQNLLINKEGNLKLGDFGLARAFGIPLRAYTHEVVTLWYRSPEILLGGKQYSTGVDIWSMGCIFAEMCNRKPIFSGDSEIDQIFKIFRILGTPNDNVWPDVVYLPDFKPTFPKWRKQDLINVVKYLDNNGLDLLQQMLIYDPINRISAKRALQHPYFQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R841</accession>
    <name>A0A1E5R841_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Histone H2B.1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g3194</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the histone H2B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R841"/>
    <dbReference type="FunCoup" id="A0A1E5R841">
        <property type="interactions" value="1183"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R841"/>
    <dbReference type="InParanoid" id="A0A1E5R841"/>
    <dbReference type="OrthoDB" id="10254238at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000014">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="13">
        <location>
            <begin position="15"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ83080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="125" mass="13623" checksum="664FE98F4464BEFD" modified="2017-01-18" version="1">MAADKKPASKAPAKKTASSAEGTKKRSKVRKETFSSYIYKVLKQTHPDTGISQKSMSILNSFVNDIFERIANEASKLAAYNKRSTISAREIQTAVRLILPGELAKHAMNEGTRSVAKYSNSVSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R863</accession>
    <name>A0A1E5R863_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Monothiol glutaredoxin-5, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3283</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R863"/>
    <dbReference type="FunCoup" id="A0A1E5R863">
        <property type="interactions" value="1007"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R863"/>
    <dbReference type="InParanoid" id="A0A1E5R863"/>
    <dbReference type="OrthoDB" id="415696at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015036">
        <property type="term" value="F:disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03028">
        <property type="entry name" value="GRX_PICOT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000005">
        <property type="entry name" value="Glutaredoxin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033658">
        <property type="entry name" value="GRX_PICOT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004480">
        <property type="entry name" value="Monothiol_GRX-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00365">
        <property type="entry name" value="Grx4 family monothiol glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293">
        <property type="entry name" value="GLUTAREDOXIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293:SF16">
        <property type="entry name" value="GLUTAREDOXIN-RELATED PROTEIN 5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="7">
        <location>
            <begin position="54"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ83078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="152" mass="16719" checksum="4F63315D03F6196F" modified="2017-01-18" version="1">MFALQKQLLNSSRLLRARPSGLSTTSLFGASLAKRFLSDETRKAIEDAISSAPVVLFMKGTPEFPQCGFSRATIQMLGQQGVDPAKFAAYNVLEDPELRNGIKEFSEWPTIPQLYVNKDFVGGCDIIMSMSKSGELAQLLDESNALVPEEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R876</accession>
    <name>A0A1E5R876_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Serine/threonine-protein kinase RAD53</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3421</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHEK2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R876">
        <property type="interactions" value="466"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R876"/>
    <dbReference type="InParanoid" id="A0A1E5R876"/>
    <dbReference type="OrthoDB" id="10252171at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009202">
        <property type="term" value="P:deoxyribonucleoside triphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016256">
        <property type="entry name" value="Ser/Thr_kinase_Rad53"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000661">
        <property type="entry name" value="Ser/Thr_PK_RAD53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FHA" evidence="8">
        <location>
            <begin position="96"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="265"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="8">
        <location>
            <begin position="733"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="438"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="894"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="438"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="895"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="387"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="271"/>
            <end position="279"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000661-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000661-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ83107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="916" mass="104527" checksum="886D123E3AF1F6B3" modified="2017-01-18" version="1">MNNLXLPANDIVTADTNTNEINDNEKKNINENLVTQPTQQATQATQKNGVERFTQDKLDDNIVCRIICTSGEVPIRDLRADFEQVSREISSVKHKWSFGRNAHAVDFHLGNIARLSNVHFYIWLGEDGNLMIRDNSTNGTWCNGSRLERGQNYMLSQGDEITVGFYVSEDVLSFIVFVNENYKKKLQLYEINERERQREVEDXNKKKRTFVEMDLEGKNADLNTRVALPHGSKSGDEHGAMQVRTSYKNGHAHDTRLTGIHKDFSVQDEVVGQGAFATVKKAIERSTGRTFAVKIINKRKLIGTKLDGVQRELEVLKILKHPRIVMLKAYYECADNYYLVMEYVPGGDLMDFVAANGAIDEDAAREISRQILQAIEYMHKMKISHRDLKPDNILIEQDDPVLVKVTDFGLAKAQNNGTIMKTFCGTLAYIAPEVVMEQEHTTPTRDNHKNKDNNDNNNNVXKRLHRLDNEYSPLVDMWSMGCLVYVLLTGHLPFSGGTQHDLFRQIKNGAYHAIPLRDNRVSEQGINFIEALLQVDPLKRLDATEALKHEWIRGNNAIDGAEYISSVPLSQNLRGDPRKTNQTTEQYINNHNNNDNSDSNGYNIRDRFQNHQDDDALVELNKKFKIPNIPVQRFTQPPKTELKSPFELKLKLKKDIGTGDANVVDGDDKRLDPIGISNDKQSYPFGIIPDTYGFTKEESKGIFIRFDASSYTILEKFNLPKSFNILQGQQRGFFVGRDSVCDLSFPNLAVLSKVHFMIIKRRHAVANSSMYESAAAALDDVWLYAFGTNAMYVNNKKLSKGYKCKVYNDDIIKFVWDKRQSMEITYRLRFVDNTGLYMHGSPPSEPEHTPSLCCPPEILAINDGFNAQFYEYALQNRPMHKQNEQEKEIPTALMNKHKLSNQKRPMKKTNVKSESN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R8E9</accession>
    <name>A0A1E5R8E9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g3490</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R8E9"/>
    <dbReference type="STRING" id="56408.A0A1E5R8E9"/>
    <dbReference type="InParanoid" id="A0A1E5R8E9"/>
    <dbReference type="OrthoDB" id="3972902at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13145:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCHF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13145">
        <property type="entry name" value="SSM4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="111"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="208"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="245"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="377"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="611"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="650"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="759"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="803"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="14">
        <location>
            <begin position="12"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ83141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="902" mass="104751" checksum="8AC9CBBB50932E21" modified="2017-01-18" version="1">MTVDLISISDNQQEQPQKICRVCRDDEGEEPLFSPCHCKSFIHQQCLVDWLKSKNGVQDSSDINYDAHCEICLGKIHFKREYTTTSANVGFVKIIKLKWHTVLQYLNCTKYVVFLLNFLWFLVNWFVIRKYFVTKQLPRWEPSYVFENNSFFVDDLTIASQQLLFGASVLTVLWSNKMGARLVNHHFGKPIDTEIPIPKIGITFKLNFCLFLAAPLLVPLCLLVFGAVPIYFTKLFFRTNHFSKFSNHIFGSLMICLLASIVPVQTFSKSNERLFTVLFYTKYYAKLAAIQYIQFLSFAMVLKPAIDRVFGDLLFGVDAHPIPISDLSLKALFMSPYSSFAFLVLNIMQFFNASFFRPGAFYFFPKFGDESSTDDSIYLFGIFWHLWSTFEDVLSMIAVFFLLYGASLRLLVDFKPDLFPISLGFFLPKNQNTGGSTVGDNNSFVFTNLDKLLVILGPLLAKFSDFYFKPFAKLAARKLRLTSYLFGQGYIDERGYVVYRNWKQQLFNPTMAALSNSTLYSDPQTKTELCTNFERNALVNAYFVPNGNFARVPGFLPMDYTERYFVPVTRKDKLLGDEPEKLVSDYKQLRNIKTYNYDVVYIPPLFKLRLFFFYIVMGSICNMTFLYFTISSNYLGKQVLKLLQFAPHIYQYIDSTTCFTLTNVVLGLFLQLTLINSLCEKKIPKYVFENVLKAIMYPVMLYVFIKVLTVFVFAFSGCISYNFVCAYDLFYKSFIRGEKRIMNVDHFYFIFPRYLFDEYMFHAFLPVLLSTSVITFKFFIQVANENRPKTVMKNFWNVVVKPTLYLFTRYFGISLVVQVFYVLLKNFKAIKSFADVQAYMGAMFSVQMHSWGNGAFLLIGPAVFSFEATRDFWKTCSNYWAKSKHQAMDKLNAEKIFLQDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA70</accession>
    <name>A0A1E5RA70_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Serine/threonine-protein kinase ATG1</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Autophagy-related protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Serine/threonine-protein kinase atg1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g2831</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA70">
        <property type="interactions" value="131"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA70"/>
    <dbReference type="InParanoid" id="A0A1E5RA70"/>
    <dbReference type="OrthoDB" id="3972846at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048941">
        <property type="entry name" value="ATG1-like_MIT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022708">
        <property type="entry name" value="Atg1-like_tMIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12063">
        <property type="entry name" value="ATG1-like_MIT1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21127">
        <property type="entry name" value="ATG1-like_MIT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0072">Autophagy</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="14"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="332"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="478"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="551"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="660"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="874"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1133"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="354"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="478"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="551"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="600"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="660"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="891"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1157"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ83798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1192" mass="134497" checksum="1DFB82EBB7D73C90" modified="2017-01-18" version="1" fragment="single">MSQNNEQVLIQNRYILGKEIGKGSFAKVYKGYDNYYTENTTTLKDDNKNSENDSSRNNSRKIKYKYNVAIKSVAKSALKNKKLLDNLEVEIAILKKINNPHIVKLIDCERTSTNFFLIMEYCSLGDLSFLIKKKDQLKKNHPLIHKIFEKYPTNDYGGLHQVLVYNYIQQLSTALKFLRSKNLVHRDIKPQNLLLDAPLSNYSTSYEFHEILHYVGISHLPILKIADFGFARFLPNTSMAETLCGSPLYMAPEILSYQKYNAKADLWSVGGVLYEMCCGKPPFRANNHMELLKKIKQANDIISFPPGSFSTSNNNTNENNNRKLFKAYNKKAVDNSETESDSDSETGNLSNSDNGDDDDDDDDNDGKQNYLSNSFSSSLTDGGSNIKKTSKQNDDENDILNHQMKMLICSLLTYDPVSRISFEEFFDNPIITKDLSVYEYDNEKDKAEESIIEQESHKMLESNLFISEYLHKKKNHTDHILQSHKHQKPEEVTKQQLPNNVPSPIAEESENESVXNNSLKNSAENIENEYVVIEKKTVEFNELADEVASYKNNPYNKSSKNTYSNPITRRFSNSRRSSASSISSVVSGTPTNIQKDDNQNNHSNVSTINSLVTPQPRKRSSNASSSSRRSSLDHRRLSISSLNPSNALSKALGIASSRLFSTGTPTSINHSSTPINTKEMYPKGSSYGSTGNSPRHELQNYGASPYAQSPANYTNVYNPSGYQRQLNSNSPSLLNSHTFTELTEQLVLAATQKQSLKGNTVEMHDITRAKAVVVEDLNVVDIINMLENLAAKASTIYLFAEVKYGQLFPEPPVEQQNKTPRHTSDYLGGAIKGNLPASTYSNKIVPNVPATNCRTSISSSKSGVSLTNASNLKASPYHHLPTNSDNSCAIEDSEDEDEEEEEEEEEEEEEEEEEEEHYSSPHQVSLSNQQYATTTDMHRVSQNMSATSVCTDDMSLLKTAPFLSNESKYVEKVKLNSEQSYHLATEALLLYLKALEFLAKSMSLTSRWWSIHQTKNMSLETNQYGENDMSSNKEMLKLNTLVQFLRNRFNESLDKADRLKDFTNRLQSKYPNIEQSPGDEIRVEKLIYERALELSKISANLEMKNCFYYSNNCELLYASSLWMLESLLDEEPASDDGKYGNGANTVNEEVGGETDKVNTQSNTATDIQSTSKLDSNDQEMIKKYITGIARRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAN4</accession>
    <name>A0A1E5RAN4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL38</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L38</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2586</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic ribosomal protein eL38 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAN4"/>
    <dbReference type="FunCoup" id="A0A1E5RAN4">
        <property type="interactions" value="885"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAN4"/>
    <dbReference type="InParanoid" id="A0A1E5RAN4"/>
    <dbReference type="OrthoDB" id="3972595at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022618">
        <property type="term" value="P:protein-RNA complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.720.90:FF:000002">
        <property type="entry name" value="60S ribosomal proteins L38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002675">
        <property type="entry name" value="Ribosomal_eL38"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038464">
        <property type="entry name" value="Ribosomal_eL38_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10965">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10965:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01781">
        <property type="entry name" value="Ribosomal_L38e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6 7" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ83964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="78" mass="8885" checksum="4115E029412F3B64" modified="2017-01-18" version="1">MAREVSDIKEFLELTRRADIKTATIKINKKVNAKGKSFRQTKFKVRGSRNLYTLIVNDAAKAKKLIQALPPTLEVQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAN8</accession>
    <name>A0A1E5RAN8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ethanolamine-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="10">2.7.7.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">CTP:phosphoethanolamine cytidylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g2582</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.14" evidence="10"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAN8">
        <property type="interactions" value="561"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAN8"/>
    <dbReference type="InParanoid" id="A0A1E5RAN8"/>
    <dbReference type="OrthoDB" id="40021at2759"/>
    <dbReference type="UniPathway" id="UPA00558">
        <property type="reaction ID" value="UER00742"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004306">
        <property type="term" value="F:ethanolamine-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044608">
        <property type="entry name" value="Ect1/PCYT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780:SF2">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="12">
        <location>
            <begin position="64"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="13">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ83968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="394" mass="45618" checksum="B71E7F67A6D0704E" modified="2017-01-18" version="1">MCDTGANCYEKVWIDGCFDFTHFGHCMAILQTRRLFPELTFNMFERSSKEENSKDSRDSDDKDGQELVIGIHNDEDIMHNKNGHPVMHEEERYYHISQVRWCSAIYTNAPYVTEPSVMDEQLCQYVVHGDDIVLDANGEDCYTKVRKIGRFKMVKRTEGVSTTEIIHRILNHTEGKHLSXDNAKDKGDPIEVDLSYKDRPFAKTLAQQGINVKMLEALSSDVNGFDPWCFVYYQNFLQPVLEGGFKFXGNKIVVFIDDLYDFDLFHAGNIEIIEYVKEEMCPDKAMFLGVGLDNDISXXFNENIKPKMINTLSERCYNVLSYKPVDGLIIKPQLTLLHGVSVVRYSEVLAQLENSSKHSLDFEYLKRDTIIKRVEQDRQQYIVRNLKKGMTYEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAY6</accession>
    <name>A0A1E5RAY6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Periodic tryptophan protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2960</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAY6">
        <property type="interactions" value="1525"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAY6"/>
    <dbReference type="InParanoid" id="A0A1E5RAY6"/>
    <dbReference type="OrthoDB" id="270624at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044285">
        <property type="entry name" value="PWP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14091">
        <property type="entry name" value="PERIODIC TRYPTOPHAN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14091:SF0">
        <property type="entry name" value="PERIODIC TRYPTOPHAN PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="260"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="38"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="525"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="530"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ84056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="545" mass="61949" checksum="AEE0A4AA755C42B5" modified="2017-01-18" version="1">MISSTCWVPKGFAKEFPESYELNDEEMERINKMAEQMNLNESENFKPLTKKDITQDQESEEFKYADDEDLKKFNLDKYDDDEEDDNDEITEASNYQQADANFDGSILPGTTQIEQDGQHYLELPNVKDNNNADSDHEEDDIILQEEEKQDLQIYKTDNLVLATRTDMEGDLSYLDVYVYDDGDDNQEYARESSLYMHHDVMLPAFPLCVEWINYKPNPNSEEIANNXDNLGNFAAIGTFDPNIEIWNLDCVEKTFPDAILQGHKDAILSLSHNEIHRNVLCSTSADSTVKIWDLNNTSKCVETFKKIHGKSKVSCSQWNNFELLTAGYDSRIALTDVREGAKSSRYWKISSNEELETIKFSADKTHVLXGTDNGNVYCFDTRNPNSSPVWTLKAHDAGLSCLNINQTIDNMFVTAAMGEKVVKIWKYENNRPKLVTTRDFGIGNILTTSFAPDFEVSGNLVVGGVSGGLQLWDTFTNGSVRKQFSKEFTNIFTNNASDSPINRFPRFSSKYKNNSAPKEVVFAAEKAEDMNEDDDDAEEIESDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB01</accession>
    <name>A0A1E5RB01_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Casein kinase II subunit beta</fullName>
            <shortName evidence="3">CK II beta</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2604</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 3">Regulatory subunit of casein kinase II/CK2. As part of the kinase complex regulates the basal catalytic activity of the alpha subunit a constitutively active serine/threonine-protein kinase that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the casein kinase 2 subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB01">
        <property type="interactions" value="694"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB01"/>
    <dbReference type="InParanoid" id="A0A1E5RB01"/>
    <dbReference type="OrthoDB" id="3971593at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032545">
        <property type="term" value="C:CURI complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030291">
        <property type="term" value="F:protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060962">
        <property type="term" value="P:regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006356">
        <property type="term" value="P:regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006359">
        <property type="term" value="P:regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.20:FF:000001">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1820.10">
        <property type="entry name" value="protein kinase ck2 holoenzyme, chain C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016149">
        <property type="entry name" value="Casein_kin_II_reg-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035991">
        <property type="entry name" value="Casein_kinase_II_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000704">
        <property type="entry name" value="Casein_kinase_II_reg-sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01214">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00472">
        <property type="entry name" value="CASNKINASEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM01085">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57798">
        <property type="entry name" value="Casein kinase II beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00045899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ84080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="296" mass="33985" checksum="AA84287B9D5A8000" modified="2017-01-18" version="1">MTSSVSSDSSMQFEEWVAAFVGRYGHEYFCEVPLEFIEDDFNLTGLSSQVKYYRKALDMITDMEVADEDMSDXDEFLDEEMVDQDGNTKKYTTXELKLEKLQNKKNMIEHCAAQLYGLIHARYILTKDGLQAMAEKYDNKHFGCCPRFLCSGMQLLPCGMHDGLGKSTVKLYCPGCKDIYVPRSSVHAYLDGAYWGSSFPGVFLNNFKELDEYCDNRKNTLVEEQKQLGNGNSSKSSSGHNSSRALQYNLKVFGFKIHDSAPSGQRMKWLRQWPSTPEEWEEFKKCEFQIPKLEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB14</accession>
    <name>A0A1E5RB14_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">dolichyl-diphosphooligosaccharide--protein glycotransferase</fullName>
            <ecNumber evidence="6">2.4.99.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AWRI3579_g2600</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a di-trans,poly-cis-dolichyl diphosphooligosaccharide + L-asparaginyl-[protein] = N(4)-(oligosaccharide-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl)-L-asparaginyl-[protein] + a di-trans,poly-cis-dolichyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22980"/>
            <dbReference type="Rhea" id="RHEA-COMP:12804"/>
            <dbReference type="Rhea" id="RHEA-COMP:12805"/>
            <dbReference type="Rhea" id="RHEA-COMP:19506"/>
            <dbReference type="Rhea" id="RHEA-COMP:19509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:50347"/>
            <dbReference type="ChEBI" id="CHEBI:57497"/>
            <dbReference type="ChEBI" id="CHEBI:57570"/>
            <dbReference type="ChEBI" id="CHEBI:132529"/>
            <dbReference type="EC" id="2.4.99.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endomembrane system</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the STT3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.18" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RB14"/>
    <dbReference type="FunCoup" id="A0A1E5RB14">
        <property type="interactions" value="1005"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB14"/>
    <dbReference type="InParanoid" id="A0A1E5RB14"/>
    <dbReference type="OrthoDB" id="10261066at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004579">
        <property type="term" value="F:dolichyl-diphosphooligosaccharide-protein glycotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043687">
        <property type="term" value="P:post-translational protein modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003674">
        <property type="entry name" value="Oligo_trans_STT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048999">
        <property type="entry name" value="STT3-PglB_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048307">
        <property type="entry name" value="STT3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872:SF1">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT STT3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02516">
        <property type="entry name" value="STT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21436">
        <property type="entry name" value="STT3-PglB_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="142"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="170"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="298"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="360"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="386"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="410"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="452"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Oligosaccharyl transferase STT3 N-terminal" evidence="17">
        <location>
            <begin position="14"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="STT3/PglB/AglB core" evidence="18">
        <location>
            <begin position="521"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF21436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OEJ84084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="733" mass="83287" checksum="9AB3C3895F165CB1" modified="2017-01-18" version="1">MVDKISLLKTVSTVLKVVIFICIIGAATSARLFSVIRFESIIHEFDPWFNFRATKYLVANGFHKFLNWFDDRTWYPLGRVTGGTLYPGLMTTSGLVWHILNNYLGLPIDIRNVCVLFAPAFSGLTAIATYYFTKACYHNDEIAGFLSAGFIAIAPGYISRSVAGSYDNEAIAITLLMITFFFWIKAMKTGSIAWGTFAALAYFYMVSAWGGYVFITNIIPLHIFILLLMGRYNHKLYSAYCTWYCIGTLSSMQIPFVGFLPIRSNDHMAALGVFGLLQFFMIGDYIKSKISNDNFKVFLKTSVLLLILFGVFGVVGLTYFGLIAPWTGRFYSLWDTDYAKIHIPIIASVSEHQPTAWPSFFFDTQFLIWLFPCGVFILFSELQDEHVFIIAYSVLCSYFAGVMVRLMLTLTPIICVSAAIALSNVFHGYLRVRNDLSGQGMKLKGFANFFKISTKLIVSSTFLGYLYLFVHHCTWVTSNAYSSPSVVLLSGQTNEDGSPVYIDDFREAYAWLRFNTKEDAKVAAWWDYGYQIGGMADRTTLVDNNTWNNTHIAIVGKAMASPEKKAYEIYKQHDVDYVLVVFGGLLGYSGDDINKFLWMVRISEGIWPEEIHEPNYFTADGQYRVDEAASETMKNSLMYKMCYHDFPSLFGNSQEGNDRVRNAKIKSSHVYDLDYFEEAFTSENWLVRIYKVKDLDPEGRSWKEVSDFEKESSATGGNSRRVVKKPKIEPRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB36</accession>
    <name>A0A1E5RB36_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3039</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RB36"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ84104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="219" mass="25066" checksum="3917988C096FDD3C" modified="2017-01-18" version="1">MAQKSSTXSRLVTTSLLYNDIPDPFSFQHHFYNENDVTFDCFKKSPTPHQKSLSDSFSLEEHCLDLSKKNLSTQEDDLFANYTFFDNLEKDFLLSNIASDEKTVGLALCNKKTTKPSQANLKHDQNKLIASTQMQQDNGRLLEDSLGLWLESFYIDSEKSLQEAKTAAMFQKMIMKQLFRSRKARGSVSKKHRKKLIQKSLVSEFHKTSDGLVPLVCNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB51</accession>
    <name>A0A1E5RB51_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL13m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2638</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB51">
        <property type="interactions" value="758"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB51"/>
    <dbReference type="InParanoid" id="A0A1E5RB51"/>
    <dbReference type="OrthoDB" id="274622at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1180.10:FF:000007">
        <property type="entry name" value="50S ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_uL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_uL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005823">
        <property type="entry name" value="Ribosomal_uL13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023563">
        <property type="entry name" value="Ribosomal_uL13_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_uL13_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01066">
        <property type="entry name" value="rplM_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL13M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002181">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00783">
        <property type="entry name" value="RIBOSOMAL_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8 9" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ84124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="159" mass="18206" checksum="DD026C46E71CCFBB" modified="2017-01-18" version="1">MSQKFGHTGLAFARVWHLVDIAKEERSLGRLASQIAITLCGKHKPIYHPTQDVGDYVVVSNCQDLRITGKKFEQKTYWRHSGKPGNLKLLTMKEMAERKGYGELLRKAVSGMLPKNKLRKTRLARLKVFDGSEHPYKQNIVAHVDQQPTIMEQLQXIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBA7</accession>
    <name>A0A1E5RBA7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2940</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBA7"/>
    <dbReference type="FunCoup" id="A0A1E5RBA7">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBA7"/>
    <dbReference type="InParanoid" id="A0A1E5RBA7"/>
    <dbReference type="OrthoDB" id="410198at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052564">
        <property type="entry name" value="N-acetyltrans/Recomb-assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43451:SF1">
        <property type="entry name" value="ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43451">
        <property type="entry name" value="ACETYLTRANSFERASE (GNAT) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ84176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="25492" checksum="E6FA38BAF1FF7819" modified="2017-01-18" version="1">MTITEPTLTYTDDFERAAHTLNKAFKDSRSNDYLMKKFFNIPLTENVSQGRIKAILEYFAAFYHDNGAEIAEANDFNTVAIYTTPEKPVDQWMTNDSRFNQSMFLDLGHKKHEIIPDHIKYYYLFIVGRDLTDTSSKKKGALRKIFETYKQKADDSNSAIVLEAISESAKSVYEYFGFKSYLKFNYGKGEVNAQGEPDKNGCGFDAYLMIYYKDTFPGFLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBK1</accession>
    <name>A0A1E5RBK1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATP synthase subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g3021</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBK1"/>
    <dbReference type="FunCoup" id="A0A1E5RBK1">
        <property type="interactions" value="1063"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBK1"/>
    <dbReference type="InParanoid" id="A0A1E5RBK1"/>
    <dbReference type="OrthoDB" id="9805536at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18113">
        <property type="entry name" value="ATP-synt_F1_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18116">
        <property type="entry name" value="ATP-synt_F1_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01132">
        <property type="entry name" value="F1-ATPase_alpha_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.150.20:FF:000001">
        <property type="entry name" value="ATP synthase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000001">
        <property type="entry name" value="ATP synthase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004039">
        <property type="entry name" value="ATP synthase subunit alpha, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.150.20">
        <property type="entry name" value="ATP synthase alpha/beta chain, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01346">
        <property type="entry name" value="ATP_synth_alpha_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000793">
        <property type="entry name" value="ATP_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038376">
        <property type="entry name" value="ATP_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033732">
        <property type="entry name" value="ATP_synth_F1_a_nt-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005294">
        <property type="entry name" value="ATP_synth_F1_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00962">
        <property type="entry name" value="atpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009884">
        <property type="entry name" value="PRK13343.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48082">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48082:SF2">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00306">
        <property type="entry name" value="ATP-synt_ab_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039088">
        <property type="entry name" value="F_ATPase_subunit_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="6 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 13" id="KW-0139">CF(1)</keyword>
    <keyword evidence="5 12" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="16">
        <location>
            <begin position="65"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="14">
        <location>
            <begin position="187"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase alpha subunit C-terminal" evidence="15">
        <location>
            <begin position="417"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ84275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="546" mass="58940" checksum="49FD513A456FA4DF" modified="2017-01-18" version="1">MLSRSTIRAATALNITKRTVPKFVKPAMMVAGRRFASSLKAQPTEVSSILEEKIRGVSEEANLNETGKVLSVGDGIARVFGLNNCQAEELVEFASGVKGMALNLEPGQVGIVLFGSDRLVKEGEVVKRTGKIVDVPVGPGLLGRVVDALGNPIDAKGPIKSVGRSRAQVKAPGILPRRSVHEPVQTGLKAVDALVPIGRGQRELIIGDRQTGKTAVALDTILNQKRWNSGSDESKKLYCVYVAVGQKRSTVAQLVQTLEQHDALKYSIIVAATASEAAPLQYIAPFTATAIGEWFRDNGKHALIIFDDLSKQAVAYRQLSLLLRRPPGREAYPGDVFYLHSRLLERAAKMSEKHGSGSLTALPIIETQGGDVSAYIPTNVISITDGQIFLEAELFYKGIRPAINVGLSVSRVGSAAQVKALKQVAGSLKLFLAQYREVAAFAQFGSDLDASTKQTLARGERLTQLLKQSQYSPLAAEEQVPLIYAGVNGYLDNVDLSRIAEFEASFLSYLQSSHENILSVIREKGELSKELLADLKSATESFIATF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBK2</accession>
    <name>A0A1E5RBK2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Methylenetetrahydrofolate dehydrogenase [NAD(+)]</fullName>
            <ecNumber evidence="12">1.5.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g2518</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes oxidation of cytoplasmic one-carbon units for purine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.15" evidence="12"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBK2"/>
    <dbReference type="FunCoup" id="A0A1E5RBK2">
        <property type="interactions" value="285"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBK2"/>
    <dbReference type="InParanoid" id="A0A1E5RBK2"/>
    <dbReference type="OrthoDB" id="41403at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004487">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01079">
        <property type="entry name" value="NAD_bind_m-THF_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000255">
        <property type="entry name" value="Methylenetetrahydrofolate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000012">
        <property type="entry name" value="Methylenetetrahydrofolate dehydrogenase [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035812">
        <property type="entry name" value="m-THF_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF3">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE DEHYDROGENASE [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic" evidence="14">
        <location>
            <begin position="10"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding" evidence="15">
        <location>
            <begin position="151"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00074830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ84280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="341" mass="37902" checksum="A20363D0B4A80979" modified="2017-01-18" version="1">MSFAGRTILASSIAKTFNEEIIAKVKTLVASNPTHRKPLLVGFLANEDPAAESYAKWTQTTCESYGIDYELRRVANKDYLEESIFDANRDDAVDGIMIYFPVFGNAQDQYLQQVVAKEKDVEGLNHVYYQNMYHNVRYLDPATKLQKSILPCTPLAIIKILEYLKVYNTLLPQGNRLYGKTAVVLNRSEIVGRPLAALMANDGATVYSVDINNIQKFTRGQGLKFSKHQVQDLGDNTSELLKEICQDADVIITGVPVANYKFPTEYIKEGAVCINFSFHKNFNDDVKGKASLYVPSTGKVTISMLLRNMLRLIDNTNAAKGITSSSSVEGKVAAMDISKQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBL0</accession>
    <name>A0A1E5RBL0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3114</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBL0">
        <property type="interactions" value="229"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBL0"/>
    <dbReference type="InParanoid" id="A0A1E5RBL0"/>
    <dbReference type="OrthoDB" id="4151048at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050797">
        <property type="entry name" value="Carb_Metab_Trans_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668:SF26">
        <property type="entry name" value="GLUCOSE TRANSPORT TRANSCRIPTION REGULATOR RGT1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668">
        <property type="entry name" value="GLUCOSE TRANSPORT TRANSCRIPTION REGULATOR RGT1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="113"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="51"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="178"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="66"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ84285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="871" mass="101079" checksum="9A26B008EC55E05B" modified="2017-01-18" version="1">MSTNGIATILADSDETIRINNSLNRIDNIKEQNFENKIKNPENQKVVKXSGYGKRKKKTQKEELVSASSDSQNVKMEQVSNTLENKIRPKRKYGTKTTDHEGILPSGKISKTLRASQSCDRCRWKKIKCMPNDTVTSGLKDQVVDSKGHCACSNCLSSGFKCEYNDKVSRRCFSKGYTDMLESKVIELQKKVDSLMENAHQLLNTTTTPTQSSSSSSSAXSQSEFLQFETLRDEAPLQHTSXPLIGSLSVQEQIIEKRQHYFKSSVYMGTMPLNYLPEFHYKALLLPLNSNSNSNSNSNSNTTRQFNQLQICNFLQTVFEFQFDTLTPECLENYFXDESYNNKTIMTLIHQIVDFELKLFPVLYPRQDWLKFCADNIKLAKETKINTXILDNXVDDEFFVFIGFLLVVYQWFFKKFSDDDLLELIKMITTNVHSPKSVQLLMFFTYYASSLPQCSTIKFEMIDLCYTKLHTMGLYLPLYAKNIKQLDNSTKQQNISKINDELKLMIFYSYQFLQKWIHICYGIPQTDRSAMIFSSTKTMLSNMQHLDGQLPQYLKSFILLSKFLNEDMMLCSLDPLTDSDDLLEKLQFFKKNLLKYNLYHTIDEHLFTDDQLDDEMNDDLSEELLKSSDSTRQNSMIMETRFASSQDKQDLDSKLMQLRFLSTDIDASQYMEIQVTLYYLLLKLFVLQNKGVQNFELMNSVLSLYYIVYSNRFEYQDLLNNEQHPPLPCFFKMMQTMPIDSLSVIKYCVDSLQTSPLFFENDYFFIVWMNILNSIPENYKGYESEQQHLKKKTRSEGGLSFVRKPENIKKTTNYVHTNYGSAVSSPYDDQESFMPSRNAVTTPRSLKIHDPSILSPKTLKNPSEFNEIQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBQ2</accession>
    <name>A0A1E5RBQ2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNA polymerase-associated protein LEO1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2692</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBQ2"/>
    <dbReference type="STRING" id="56408.A0A1E5RBQ2"/>
    <dbReference type="InParanoid" id="A0A1E5RBQ2"/>
    <dbReference type="OrthoDB" id="20844at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990269">
        <property type="term" value="F:RNA polymerase II C-terminal domain phosphoserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007149">
        <property type="entry name" value="Leo1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23146">
        <property type="entry name" value="LEO1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23146:SF0">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN LEO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04004">
        <property type="entry name" value="Leo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="368"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="507" mass="58667" checksum="73A7E78DFC727414" modified="2017-01-18" version="1">MSEAEKEVVASSNIEEHSSDFQSQTEQGENAAAVSDSGVTEAQKEESTEIKDTEASSSEVPIAGEDDKAEETKNEGNEADKDNGDENNAEEEQIEKDQIDEEMDDLFGDGDEDEDEDEDEEEVVTNKKRSVIDDEAQDESEEEETKENYFYRNADDNEEDEQYEIKEADVTLSRHIIPYKTAGPVTSEDDALIYYVKVPQFLKIDTKPFDPQMFEEEVEERLRDPEYLREDKIDAGLIDENTIRWRYSKDENNNVSKESNAVIVQWEDGSYSLKLGEEYTDILINDIDNSLLVKSHEREELMQSVQGGFINKNMMFVPTSMNSKIHKRLTKAVHNHNAKEQQDSGPKTFIIRKDPDLELKELEKKQDQVLRERRRNQLREKMEKENALYGDQDKAQSPFSFNKRTASGYKEDYADQLLDGEEEGEEEEEEEEDDNDFIDDDGVEEEEEEEEEEEEEDDDDDARAERLRQVKKHGFSYTNDADDDERANNASPSKRRKVIVEDDEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBU2</accession>
    <name>A0A1E5RBU2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Intermediate cleaving peptidase 55</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2871</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peptidase M24B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBU2">
        <property type="interactions" value="497"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBU2"/>
    <dbReference type="InParanoid" id="A0A1E5RBU2"/>
    <dbReference type="OrthoDB" id="4215474at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01087">
        <property type="entry name" value="Prolidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007865">
        <property type="entry name" value="Aminopep_P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052433">
        <property type="entry name" value="X-Pro_dipept-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226:SF4">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05195">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01011">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminopeptidase P N-terminal" evidence="7">
        <location>
            <begin position="51"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ84359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="498" mass="56093" checksum="EBA6D542F590B7BA" modified="2017-01-18" version="1">MLTKKIFKSLLHKKLQSTLSATRPPRLINLGQPLHENRPYLVKAGDITPGISAQEYYQRRLKLLEKLPAKSCVVIPGSQIKYASGPVFYPFQQNNDLFYLTGWNEPDSVMILEKLNENEHDSVLHMLVPPNNKAKEQWEGHRTGVENVREFFNADESSDISHLASYLDKVISRNANVYYDFAKKPNTFATMFAESSAASNQNITTIENIARHHNKSIRGVKTLIADLRVVKSPSEIAIMRRAGQISGRAYNMAYAERIANERSLHAFLEYNFISGGCDKSAYVPVVATGSNALCIHYTSNNDVMYNDEIVLVDAAGSLGGYCSDISRSWPVNGXFSQPQRDLYEAVLNVQKKCIDMCKTENNLSLHDIHSWSSRYMIEELKHIGLHNADTYINVLYPHYIGHNLGLDVHDVPECSRFAQLLENQVITIEPGVYVPDDPRFPEWFHNIGIRIEDDIAIGKNDYLNLTVEAAKEVVDIESIAKNGVTTKIPKDVISPLNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBZ9</accession>
    <name>A0A1E5RBZ9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">E3 ubiquitin-protein ligase complex SLX5-SLX8 subunit SLX8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3038</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBZ9"/>
    <dbReference type="STRING" id="56408.A0A1E5RBZ9"/>
    <dbReference type="InParanoid" id="A0A1E5RBZ9"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033768">
        <property type="term" value="C:SUMO-targeted ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032183">
        <property type="term" value="F:SUMO binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140082">
        <property type="term" value="F:SUMO-ubiquitin ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049627">
        <property type="entry name" value="SLX8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47094">
        <property type="entry name" value="ELFLESS, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47094:SF1">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="128"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ84103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="194" mass="21286" checksum="A2F6AF313B95C6F1" modified="2017-01-18" version="1">MAEDISLAELSTSQANTEDVHGGTVASSSSNNKRARISSVESADFEELQIVTKKQKHAGNTLPDATTNQQQQALDSDELVVIAEDIRNEQVGEAIDDDDIEIIEKPSVPRDKSKERGDVTKSITQYQCPICLEPPSPAMITKCGHVFCTDCLFNMLNSSRTQRLAGTCALCRTKIPLNKTNLMVMKFTTVKKPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDF4</accession>
    <name>A0A1E5RDF4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Regulator of Ty1 transposition protein 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1933</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat WDR6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDF4">
        <property type="interactions" value="653"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDF4"/>
    <dbReference type="InParanoid" id="A0A1E5RDF4"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051973">
        <property type="entry name" value="tRNA_Anticodon_Mtase-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14344">
        <property type="entry name" value="WD REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14344:SF3">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="180"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="222"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ84935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1007" mass="113344" checksum="8182CD2FBD317ABA" modified="2017-01-18" version="1">MQLEKKYHTGPSYAAKFYAPLNHIWTAEGPLLKIYDQITGELVNSCRVFSRNKIHGILLNGKSNVVLYGATNYVFTSADELYSNTDLSSQEQRNCEWITSGALSEDNKAYLLTCYNKVLIFDFDQQKHIETKALAGERSILYSGSIKLGSSTXGPIVCAGTVMGGVLVWDCNKEXLLHNLKGHEGSIFDAVCSDNFDKVASCSDDRTIRVWNIQDGSELCFAFGHTARIWSLKFINDDTQIVSTSEDCTMRVWDITKTXENTYSLEQSSVHETNQIKNVWGCDVSDSRIXTTGNDGRIKITNLLNIGEEGRNLSLFSLENITSQSRKPFIAKEMFKSFQWFSFGLVVITSRGRCFQFHKTSATWAFLFENENFANYNFLQGFENICIITNSFGDIKLLKFDERGAVSKELDYNVKDKLSKTLNTLCVGKKSFIYLLLESPNPNDPLLLMKIDKNNLKNGSFVSFQKPKNFSSSCFEIYGDTLLIGSRMSTFGIFQISPSQNTPKLIKQLSPGDNTTSITFTGKYHLKNPIFSITNRDGFYNFVSVNIQTLEHVIIHSNKIAKGFLQHGNFSSNGDYIINGFKSNIFHISNESQNYEIVSESCGGAHRQWGLFEKDDGSDGKVFVYARGPLHIRDISDYTHTVQTLNNGIHGREIRDITFQPDQLNSTDVLFVTASEDTTLKVCSFNTDSGKITQYWTQRKHVSGLQRAKFINSNLLISCSGREELFLWELHKSDDGYPFLNLRESLPSASDNPDLRIMDFDFVFHKDRRDEFLLATVYSDSSIKIWYYTTKGKFQLVLDGKYETCCLFNTAFMXLEDKLVLIVTPTDGHVVYWDITEYVPFQVQDXQLVDIEVGIVDNSFKXEPLPPYLTRFPVHQSGTKTLALYKNAGIETGKTSVTLITGGDDNALSTSVFSLNNDGIVQGKRIQIIEKAAASTITSVTHFTKDNEPFCMVASVDQTLRVWKIASDNTASLVTKQYTTIADTNSLDIYNNEYAIVGGVGCSVWAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDR3</accession>
    <name>A0A1E5RDR3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ribosome biogenesis ATPase RIX7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2311</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDR3">
        <property type="interactions" value="1040"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDR3"/>
    <dbReference type="InParanoid" id="A0A1E5RDR3"/>
    <dbReference type="OrthoDB" id="27435at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990275">
        <property type="term" value="F:preribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19518">
        <property type="entry name" value="RecA-like_NVL_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19530">
        <property type="entry name" value="RecA-like_NVL_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000018">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000110">
        <property type="entry name" value="Ribosome biogenesis ATPase RIX7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000156">
        <property type="entry name" value="Ribosome biogenesis ATPase RIX7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000365">
        <property type="entry name" value="Ribosome biogenesis ATPase RIX7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="249"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="586"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="174"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="10">
        <location>
            <position position="870"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="870" mass="96387" checksum="0F8BDF03353C7E48" modified="2017-01-18" version="1">MVKVKSSKKLLSSPLEGKIEDLIYRLIEERSLEKQRDLIKKQQLKAEKLASKQSAGNVSDDSSFSDXSEVEDPYPEIFLAQDLKFGEVIGFCLSKDLSLQRVKKVLMEKTVEKMLRSVVEKEKEEIGTLXQNGQDTGAETDGVTNISVDNLVLDTDVNNTMNKGITSQWTNVVKTEESMEPPAVANKKKRTKDGHSGVSKAKKSKTDRTPPSSNLSSLGGMDDVIAQLMELIGLPILHPEIYLTTGVEPPRGVLLHGPPGCGKTSIANALAGELQVPFISLSAPSVVSGMSGESEKKIRDIFEEAKNLAPCLMFFDEIDAITPKRDGGAQREMERRIVAQLLTSMDELSLEKTNGKPVIVIGATNRPDSLDAALRRAGRFDREICLNVPNEISRLHILKKMTQSLKIDGEIDFQKLAKLTPGFVGADLKALSTAAGTCAIKRIFSKYGDNVNASIKNKEEGEGEGEXEDVNMADADELXQQEYQQELKNTADIINPLPLSIIQNFITQFPEPLNEEQLSQLSIRYEDFLKALPTIQPTAKREGFATVPDVTWRNIGALKNVRIELNMAIVQPIKRPELYERVGISAPAGVLLWGPPGCGKTLLAKAVANESRANFISIKGPELLNKYVGESERAIRQVFTRARASVPCIIFFDELDALVPRRDSSMSESSSRVVNTLLTELDGLNDRRGIFVVGATNRPDMIDPAMLRPGRLDKTLFIELPKFNEKLDILTTLIKCNGTPLGDDVDLKEIVEDERCRNFSGADLASLVRESSVLALKRKFFISDEIQSIAENDLDKEFEELTTEDEDKSGISTTKALFVTKLDFFNALKKIKPSVSDKDRLKYDRLNKKMGWIEEDAEEQKSEPQPQASD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDS8</accession>
    <name>A0A1E5RDS8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcription factor tau subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2447</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDS8"/>
    <dbReference type="FunCoup" id="A0A1E5RDS8">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDS8"/>
    <dbReference type="InParanoid" id="A0A1E5RDS8"/>
    <dbReference type="OrthoDB" id="5598268at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001002">
        <property type="term" value="F:RNA polymerase III type 1 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001003">
        <property type="term" value="F:RNA polymerase III type 2 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.160">
        <property type="entry name" value="TFIIIC, subcomplex tauA, subunit Sfc1, barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019136">
        <property type="entry name" value="TF_IIIC_su-5_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040454">
        <property type="entry name" value="TF_IIIC_Tfc1/Sfc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041499">
        <property type="entry name" value="Tfc1/Sfc1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042536">
        <property type="entry name" value="TFIIIC_tauA_Sfc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13230:SF5">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13230">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIIC, POLYPEPTIDE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09734">
        <property type="entry name" value="Tau95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17682">
        <property type="entry name" value="Tau95_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="Transcription factor IIIC subunit Tfc1/Sfc1 triple barrel" evidence="7">
        <location>
            <begin position="38"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor IIIC subunit 5 HTH" evidence="6">
        <location>
            <begin position="246"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="550"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="564"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="590"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="8">
        <location>
            <position position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ85041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="646" mass="74565" checksum="87A59B627A5894C9" modified="2017-01-18" version="1">MTDHDYDSRSPTPVPLAQNRNSLIATEYVLKDLPYMQSVEFPLKCKPGKDVPESIANMINMCGGINNIKETFQAIALSEAENSINENGEETLSNGQTAAKENAPKPLELYLNKNTAENCDGLLFNDHPLLGKPTRDQRLILKIKIKRKKTDRIMHKSKKMVDKFQDCVSYTATPVAIVDTTIKFRALSDFQVLLDNNRSAKEYDQCFNKLKWENMKSFVNSIEENDTKPFYPLFEVSQNKAAIFELLPPPRFSMVDIPYVYKFKRNPYHDVESNTVRKNYVKAHQIQVTNLDTIPDKPPQVLLDNLKVAKETGIYPGTSKEDAVFLKELESRIDDLKRLFEKQPIWIKKHIDGILSHLDTTWLQMVRFALPFVSYRIMKGPWRNTYCKYGVDPISDPKFAKYQTENFRIEKSYYTVKVEECPNKFVSDTPNEIDSRFFFTGKQIPWYSTYQMFQLEGEPNIKEIMDQVVYLDKPNDATGWFPELDLWKIRKIVKYELGCLIKGNQDFSSTKLKIIKEMPFLKGFNPNSTYPKKEKNFPYVKNKLLESSNEELGAAQQSGKSDGNNDEDDEEEDDDNGVDAGNEDLHDVAIDEELDDDENEDEFMNDKFDLTSASFKEIVQRIMVKDPELGKYMDSNIEGLVKADKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDV1</accession>
    <name>A0A1E5RDV1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1729</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RDV1"/>
    <dbReference type="OrthoDB" id="4070671at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031426">
        <property type="entry name" value="DUF4667"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15700">
        <property type="entry name" value="DUF4667"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="247"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="268" mass="29911" checksum="FEDA113822D2B9A1" modified="2017-01-18" version="1">MMQNGTRTANDVRPMSSTAGYSYPIDAKITLFRDESDEYNVNCDKSNEIGLSKSNIKDXGSRSNSSSSSSSSSSINNSRSRSRSRSRTSSQSNTSRVWSRKPSVIDIYVDKELKEHEEQLLRESFGQCYHCRMNGVNINGMNGNEVNVNGMNGNGVSGNAVSGNAINGNAVNXNTSQHCSNCNHRLRAHSISVDTPCKKHHHRRNSVCVKFQKMDTQDGREDKDLDEKNIGKXYEEXIDETQXLKFNNNNNNNNNNGNGDVQFKNPFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REC9</accession>
    <name>A0A1E5REC9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">M7GpppX diphosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2002</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HIT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REC9"/>
    <dbReference type="FunCoup" id="A0A1E5REC9">
        <property type="interactions" value="931"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REC9"/>
    <dbReference type="InParanoid" id="A0A1E5REC9"/>
    <dbReference type="OrthoDB" id="10264956at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.40">
        <property type="entry name" value="Scavenger mRNA decapping enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008594">
        <property type="entry name" value="DcpS/DCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011145">
        <property type="entry name" value="Scavenger_mRNA_decap_enz_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12978">
        <property type="entry name" value="HISTIDINE TRIAD HIT PROTEIN MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12978:SF0">
        <property type="entry name" value="M7GPPPX DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05652">
        <property type="entry name" value="DcpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11969">
        <property type="entry name" value="DcpS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028973">
        <property type="entry name" value="Scavenger_mRNA_decap_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102860">
        <property type="entry name" value="mRNA decapping enzyme DcpS N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="239"/>
            <end position="250"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR028973-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR028973-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="307" mass="35683" checksum="3D837331264F32A9" modified="2017-01-18" version="1">MTSTTPPSFEKLVKNFEFVRTLDTNPQIKTVTLLGTIFAKNAIITLEKLHFNENQTHNAAELVTDFDEVTANDVYFWSNASMNSIVGKSDLKTGDKIKINLVYPATEIHIRKYDEQKFHMIKETPEMYKSHVVPYIKEYQNAGRLNWVYNILFHGHEADRIVHNEPDQFILLPDMKWSGHDMDSLYLLAIVYRKDIASVRDLNSSHKEWLISLQNTIKRVVPEKYPQIEDASQLRIFIHYQPSYYHFHVHVVNIKHPGLRDGISVGKALLLDDVIDNLDFLGPEGYAKKNITYVLGENHPLWEKFNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REF9</accession>
    <name>A0A1E5REF9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PA14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1666</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REF9"/>
    <dbReference type="InParanoid" id="A0A1E5REF9"/>
    <dbReference type="OrthoDB" id="3973523at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000128">
        <property type="term" value="P:flocculation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001389">
        <property type="entry name" value="Flocculin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018871">
        <property type="entry name" value="GLEYA_adhesin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00624">
        <property type="entry name" value="Flocculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10528">
        <property type="entry name" value="GLEYA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184723" description="PA14 domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="PA14" evidence="2">
        <location>
            <begin position="101"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="431" mass="47252" checksum="B999E95810A1E80E" modified="2017-01-18" version="1">MKNFIIRPLLFFFTILSFFLSLITPEKSLVESTFTSGTSVGGCSAPNVAFSSFFLVDIFYYPWESYSTSTNTGTGNFCLYVSEAYLTGGYIGSGSLYSGSKTSVSIVSYYLTSTNLNFNINVPGNTETWQYMSNFQKNGQSAYVPVSNFAFLATAYLVPSQTGLYTFNLNNVDDLAIISIGDGVAFDCCCTVPYVFMQAQLGAMWTSNGVQGIKTIQLQLTQGVYYPIRVFYVNRNSQAIFDFTYTDPSGVSHSDWTNFAYQIYNLPSSYCPYGAQTTVPWTGTFTTSVAYSTETKPITAYFNGQAYVTTEYATDYAVATPISRTTTQSTTYWTGSGTTTLSVTSFLTTSNSQTYIVTSYLVATPEVSATVTRTTSYTGTVTQTYSTSYTTFAGTDGVETAQTIYFVQTPIPRTVQTLTTSWTGKVITTSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REJ0</accession>
    <name>A0A1E5REJ0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REJ0">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REJ0"/>
    <dbReference type="InParanoid" id="A0A1E5REJ0"/>
    <dbReference type="OrthoDB" id="284718at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28038">
        <property type="entry name" value="ADL329WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28038:SF1">
        <property type="entry name" value="ADL329WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="118" mass="13704" checksum="06E6A823165E3AD9" modified="2017-01-18" version="1">MSTPFXKQSKKVRKDLAVKYVHKKAPVLDNTLGQGILTQYMPLLAMFLRNKYLAWFSLITQFYYYLTTPQDLPGNKDSSSNLDLPRILMCVSSIVFCYIDLIFPNEMSFKKLQDKVQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REJ1</accession>
    <name>A0A1E5REJ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PHO85 cyclin PHO80</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REJ1">
        <property type="interactions" value="178"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REJ1"/>
    <dbReference type="InParanoid" id="A0A1E5REJ1"/>
    <dbReference type="OrthoDB" id="337735at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20558">
        <property type="entry name" value="CYCLIN_ScPCL7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF117">
        <property type="entry name" value="PHO85 CYCLIN PHO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="394"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="407" mass="44991" checksum="13BA5169C0F4EA64" modified="2017-01-18" version="1">MSEPCTKTLPQDFTKCPINDLIKLISRMXSSVIYINDTKLASTAATNSSSTSRRVSSTSAESPGSPYXPSLSSNTALTRFHSRVPPNISVYDYLVRLTKFSXLDQTVLLTTLYYIDLLSAVYPSFYINSLTVHRFLLAATTVACKGLRDVFYNNDHYAKVGGVLTTELNILESDFLDKIHYRILPRDSNLLLITHEQMSGQFSVFPSDIGLTIESENAGYNILTTYYQRMIGLVGNYWSIMHSNGLAINTXESTGSTGASXYQQKEFPCNNINYRVNEGFRTTYVLEDHSVHLNSRENTPHTVSAATSKSQQDHSGEXVVEKVSQRSEKLPATSNESSNLACTNTECPYPENADEAKTSSFGNSATPLNGVQSANNRKRILAESAHKTFPSKKVNGSANVLKQQEPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REU6</accession>
    <name>A0A1E5REU6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">1,3-beta-glucanosyltransferase</fullName>
            <ecNumber evidence="8">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2383</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Lipid-anchor</topology>
            <topology evidence="8">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyl hydrolase 72 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="8"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REU6">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REU6"/>
    <dbReference type="InParanoid" id="A0A1E5REU6"/>
    <dbReference type="OrthoDB" id="421038at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042124">
        <property type="term" value="F:1,3-beta-glucanosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071970">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000038">
        <property type="entry name" value="1,3-beta-glucanosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004886">
        <property type="entry name" value="Glucanosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468:SF10">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03198">
        <property type="entry name" value="Glyco_hydro_72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 8" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <keyword evidence="8 11" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009028104" description="1,3-beta-glucanosyltransferase" evidence="8">
        <location>
            <begin position="29"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="10">
        <location>
            <begin position="403"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="530"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="586" mass="65820" checksum="E60ACA42545FE8ED" modified="2017-01-18" version="1">MKTFKNHLGTTLLRIVLLNSCMFARARAEDVASADLPPIEVFGNKFFNSETGEQFFMKGIAYQPSTGESPDYISSMSGKKYIDPMANPNICLRDLPYLVKLGVNTVRVYSVDPTSNHDECMNVFAQNGIYVLFDLSEPDISISREAPGWDVTIFDRYKSVVDSVGPYANTLGFFAGNEVTTDKTNTLAASFVKASVRDIKKHXREHAALSRIIPVGYSSNDDPDVRDQLAAYFVCDTDNNNNVDFFGINMYEWCGYSSFVGSGYRERTLEFKNYPVPVFFSEFGXNXVRPRPFTEVEALFSPLMTNVWSGGLVYMYFEEANQYGVVSVDITSGDVKELPDFEHLQKEYNKAKPKGVTKDAINQDDAIHQASKCPDNSEMWVPKNNILPPTPNFDKCACLTKMAPCVVEQNLPRKKDYSKMFEYLCGKVDCSEISSSKGVYGEYSDCSLDQKLTYVVSKFYYSLNSQKKNNEECPQITSGTFSGIYFNQLNGNSISSCKPYFDQLKNKFEQSDDAAKTNKRKNKNETLVQPAFSPFPLNNGKKDEKKTSNKQLSNSADGTCQNTKCLKRMVIISLFTAVLGAILIYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REX5</accession>
    <name>A0A1E5REX5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cell division control protein 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2294</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REX5"/>
    <dbReference type="FunCoup" id="A0A1E5REX5">
        <property type="interactions" value="194"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REX5"/>
    <dbReference type="InParanoid" id="A0A1E5REX5"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031105">
        <property type="term" value="C:septin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="7">
        <location>
            <begin position="19"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="305"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="374"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="306"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ85416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="429" mass="48805" checksum="1F447FA6BAA15999" modified="2017-01-18" version="1">MNGIIEASANLRKRKQLKRGINFTIMVIGPSGSGRSTFINTLCGQNVVECSGASLKQNSKYLSAGNEIQAQVPVDLRHEMVELEDDEGVKIQLNIIDTPGFGSSIDNTNNFDIISDYIKHQYDEILLEESRVKRNPRFKDGRVHVLLYFIEPTGHGLKEFDILFMQKIGDLCNILPVISKSDSLTVEELKLNKKMIIDDINYYNLPIFNFLDNVNEDEISEEDYETNLYLKSLLPFSVIGSNELYTGANGEQIRARKYPWGIIDVEDANVSDFVVLRNTLLISHLNDLKEHTHQILYENYRTETLSGNSHTKSNNNNSNNYYSGSRSVDAHNTATVASTSVSSSNAATDTQGDKPHEYSNESETNHVAAAGDNYLAKEEQIKLEEERLKAFEERVQQELQLKRQELLKREQELREIEERLEREASMNNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF31</accession>
    <name>A0A1E5RF31_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Heat shock protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2408</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF31"/>
    <dbReference type="FunCoup" id="A0A1E5RF31">
        <property type="interactions" value="125"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF31"/>
    <dbReference type="InParanoid" id="A0A1E5RF31"/>
    <dbReference type="OrthoDB" id="2348401at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007250">
        <property type="entry name" value="HSP9_HSP12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04119">
        <property type="entry name" value="HSP9_HSP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002590">
        <property type="entry name" value="HSP9/HSP12_fun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="116" mass="12163" checksum="6BD9A50E87D2F3BD" modified="2017-01-18" version="1">MSDTGRKDFTDKAAEAVKPDSQKSYLEQGKEKVTDAGDKAASYLQPESNKGVGQSVSDSFGHGTDKGEAKSNTKSFGESASEYAEAAKEKISEAAEYIGSSLGGAKTDAEKTAEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF50</accession>
    <name>A0A1E5RF50_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein phosphatase PP2A regulatory subunit A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2247</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphatase 2A regulatory subunit A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF50"/>
    <dbReference type="FunCoup" id="A0A1E5RF50">
        <property type="interactions" value="524"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF50"/>
    <dbReference type="InParanoid" id="A0A1E5RF50"/>
    <dbReference type="OrthoDB" id="340346at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000062">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A regulatory subunit A alpha isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055231">
        <property type="entry name" value="2AA_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054573">
        <property type="entry name" value="PP2A/SF3B1-like_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051023">
        <property type="entry name" value="PP2A_Regulatory_Subunit_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648:SF4">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2 (FORMERLY 2A), REGULATORY SUBUNIT A, BETA ISOFORM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A 65 KDA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22646">
        <property type="entry name" value="PPP2R1A-like_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22956">
        <property type="entry name" value="VPS15-like_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="53"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="92"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="257"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatase PP2A regulatory subunit A/Splicing factor 3B subunit 1-like HEAT repeat" evidence="4">
        <location>
            <begin position="294"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="297"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="339"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="378"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="417"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatase 2A Regulatory Subunit A helical" evidence="5">
        <location>
            <begin position="498"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="536"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="579"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ85519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="610" mass="69185" checksum="AECE49687EB5C55C" modified="2017-01-18" version="1">MDLSHIDESDDLYPLALLMDELKHDDIANRVDAMKKLDTIAIALGPERTREELIPFLTEVAQDDEDEVFAVLAEMLGSFVPLVGGYQYATCLLPPLEILSSTEETIVRDKAVASLNMIAKELSQDQILHDFIPLIEHLASTEWFSSKVSACGLFQSVIIRIQDDKLRVKMFTLFYNLCIDETPMVRRAAAKNLPPIIDLLTDNMKISTDQDWEFISNMFQKIITDNQDSVKFLAVDILISILKFFNKKKNSSHLPDLLNSTIQLINDEAWRVRYMAADRFTALAENFQSKNEYLQQLTPPFLHLCEDNESDVRKAISKQIPGFAKLIVKLDPNIVLDKIIPCVQSLSMDDNETVRTALASEITQMASLMNKTECTEHLIPILLNMLRDECPDVTLNIISNLEVVNKVVGIDILSENLLPAITDLSIDVNWRVRLAIIENIPLLAKQLSVDFFEERLSALCLSWLWDSVYSIRHAAVENLHKLTEIFGSDWAKNEIINKLLKSKSQLLENFVYRITLLHALTALIPVVNDDVVLEKILPFIDHLSEDKVPNIRFNVAKSYSIVVEKLDKQKYSNLIGNTIIPKLQTLSKDSDPDVTFYASESLGKVENLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFB8</accession>
    <name>A0A1E5RFB8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Myosin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g2229</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFB8">
        <property type="interactions" value="654"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFB8"/>
    <dbReference type="InParanoid" id="A0A1E5RFB8"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15480">
        <property type="entry name" value="fMyo2p_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01380">
        <property type="entry name" value="MYSc_Myo5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046943">
        <property type="entry name" value="Fungal_Myo2/2A_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036103">
        <property type="entry name" value="MYSc_Myo5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF706">
        <property type="entry name" value="DILUTE CLASS UNCONVENTIONAL MYOSIN, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01843">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM01132">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50084">
        <property type="entry name" value="Myosin S1 fragment, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51126">
        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 9" id="KW-0518">Myosin</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="14">
        <location>
            <begin position="9"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="13">
        <location>
            <begin position="90"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="domain" description="Dilute" evidence="12">
        <location>
            <begin position="1277"/>
            <end position="1552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="672"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="9">
        <location>
            <begin position="711"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1551"/>
            <end position="1572"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="1013"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="674"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1560"/>
            <end position="1570"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="184"/>
            <end position="191"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ85591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1631" mass="186093" checksum="AA519E70AA423A01" modified="2017-01-18" version="1">MSNTKTSYDVGTRCWYPNKEQGWIGGEITKNSFDQASGLFSLELKLENNEVVKIETKNLESVEANLNATTSTDSPSKETLPLLRNPPILESTEDLTSLSYLNEPAVLHAIKTRYAQLNIYTYSGIVLIATNPFDRVEQLYTQDMIQAYAGRRRGELEPHLFAIAEEAYRLMKTDXKNQTIVVSGESGAGKTVSAKYIMRYFASVEDIHNPDQSMNSQFNTQNGDSDLDIDGIPTVDGVSLETSSTNSLTFGPEMSETEEKILATNPIMEAFGNAKTTRNDNSSRFGKYLEILFNENTSIIGAKIRTYLLERSRLVFQPKSERNYHIFYQLLSGLNDEDKEKLFLKGIDDYKYTNQGGEPVIAGIDDKEEFYTTVKSLQLVGVNXNTQFEIFKILAGLLHIGNIEITKARNDAAISSEEANLKLACDLLGLDPVLFAKWLTKKQITTRSEKIISNLNYQQALVSKDSVAKYVYSCLFDWLVENINVTLCNPEVEDQVKTFIGVLDIYGFEHFAKNSFEQFCINYANEKLQQEFNQHVFKLEQEEYVKEKIEWSFIEFNDNQPCINLIENKLGILSLLDEESRLPAGSDQTFAQKLYETLDKPPTNKVFSKTRFGQSKFIVKHYALDVEYDSEGFIEKNRDTVSDGHLEVLKSSTNKTLLEVLSCVDRAAEKLANEQEQQSQTQTQSKPVGRIGNAXTVNKKPTLGSIFKNSLVELMNTINSTNVHYIRCIKPNEEKEAWKFDNVMVLSQLRACGVLETIRISCAGFPTRWTYNEFVSRYHLLTPSKYWENLYSTDITEEDIKNLTQKVLEYTNTVPAKYQLGLTKVFFKAGMLANLERLRSDKLHESIVKIQKNVKKVYYQXQYQKLLTSIKVLQSSLNAYYVRQKVELEIKTNASLLIQSLLRSTLVKTKYNAIIESVIVIQSLLRMQQSKKLLVKQKEFDSAVKVQKNIRSFQPRKAFLVQQKSSLTLQNLIRRRQAQAKFRKMRSEAESVNSLKEVGYQLENKVIQLSETIAVKIKENKELNSRIADLQEKLLNNENLKEVLNLEKLNQSKELEFLQKSKETDGSEYKQKLLKAKEEMDKAKQEIDSIKAEKDQLKKDVQDKLTELQEARDQLGNYKTQNSDLQNEVNSLKEEIQRLQQSLQFASASSVSAKSNGVAYNNGLQETPKSNRLSISATDYIDPNTPGAYSDLYDGDDRSINSVLSQINDELYKLFDDTKALNKEIVFGLLKDFKVPEAGVAVEMSRKEVLYPARILIIILSDMWRLGLTKQSETFLAEVLSAIQNIVTNIKGDELIPNGAFWLTNVRELYSFVVFAENSILNDETYNSGLTDAEYEEYVKLVTELKDDFEALSYNIYNIWVKKLEKDLEKKVISAVVLSQSLPGFIANEGSQFLPKLFSQPIAYKMDDILTFLNNIYWSIKAYHVESEVFREVITTLLKFIDAICFNDLIMRRNFLSWKRGLQLNYNVTRLEEWCKAHYIQEGTECLVHMLQAAKLLQVKKATLEDIDIIWEICSSLKPAQIQKLISQYSVADYESPIPEEILQNVAERVKKDAAENPKKPSSSKSGSKSHSNDIFITVNTGPFDDPYATLDTREFGKIEAYIPAWLNLPITKKIVNLVTSHVKVQESGNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFC0</accession>
    <name>A0A1E5RFC0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin-D1-binding protein 1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1986</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFC0">
        <property type="interactions" value="85"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFC0"/>
    <dbReference type="InParanoid" id="A0A1E5RFC0"/>
    <dbReference type="OrthoDB" id="4088536at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1410.10">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026907">
        <property type="entry name" value="GCIP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049317">
        <property type="entry name" value="GCIP-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15492">
        <property type="entry name" value="CYCLIN D1-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15492:SF1">
        <property type="entry name" value="CYCLIN-D1-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13324">
        <property type="entry name" value="GCIP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-D1-binding protein 1-like N-terminal" evidence="2">
        <location>
            <begin position="149"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="411" mass="46347" checksum="EFCDA5144A9162AA" modified="2017-01-18" version="1">MAQPQDVTKESLLDISTGLRTNLQAFAQKQDLHTKVEKNFDKSKNQHVNELEELAKLAKLIKAHTTKLGILLSKFGESTKVDQKKNTVEQDLKKLGAPLLTEITDLNSSVWYLLSLLHLIHANTISSSLSKEKLPKGKNCYPKVYADAIDENVLYLVQNVEELISIVQRXLANDTNEQKTIDEISQQRLISVGKIWASCDKLVALSLSIPKVIIDYINSVTMKLLSDTLVEIEEWLEDPTLEDLDDPFGLDYTDEEESESDNETHTGTKDAPSPEMIEFMELWNTKIRLIKLLMNSLKLSLTKATSSTGTANSANTNVSGLPAKMCLHLQIIYELQKQITLNVDDLISTVFMSNNKDFVDPMKDEDIKNMITELNENILVKICNTVIALNTDSAKSEDTKKWIATWKSKYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFF7</accession>
    <name>A0A1E5RFF7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sphingolipid long chain base-responsive protein PIL1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2265</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFF7"/>
    <dbReference type="FunCoup" id="A0A1E5RFF7">
        <property type="interactions" value="493"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFF7"/>
    <dbReference type="InParanoid" id="A0A1E5RFF7"/>
    <dbReference type="OrthoDB" id="5599269at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036286">
        <property type="term" value="C:eisosome filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000005">
        <property type="entry name" value="Sphingolipid long chain base-responsive pil1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028245">
        <property type="entry name" value="PIL1/LSP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31962">
        <property type="entry name" value="SPHINGOLIPID LONG CHAIN BASE-RESPONSIVE PROTEIN PIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31962:SF1">
        <property type="entry name" value="SPHINGOLIPID LONG CHAIN BASE-RESPONSIVE PROTEIN PIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13805">
        <property type="entry name" value="Pil1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="272"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="279"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="319" mass="36415" checksum="C4F07FA40005C602" modified="2017-01-18" version="1">MHRTYSLRNQRAPTASELQNPPPPPSSTKNKFFGRGGLAYSFRKNAAGSFGPELSRKLNTLVKMEKNVLRSLEMVANERREAAKQLSVWGLENDDDVSDITDKLGVLIYEISELDDQFIDRYDQYRLTIKSIRDIEGSVQPSRDRKAKLADKIAYLKYKDPQSPKLEVLEQELVRAEAESLVAEAQLSNITRSKLRAAFNYQFDSIIEHSEKLALIAGYGKAMLELLDDSPVTPGETRPAYDGYEASKQIIMDAEGALNNWTLDSAQVKPTLSFRSDYYDEEEEEEELPEEEEEEEEELPEQHEVEEDEHYEEEPVVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFH3</accession>
    <name>A0A1E5RFH3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histone promoter control protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1779</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFH3"/>
    <dbReference type="InParanoid" id="A0A1E5RFH3"/>
    <dbReference type="OrthoDB" id="3973281at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014840">
        <property type="entry name" value="HRD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08729">
        <property type="entry name" value="HUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hpc2-related" evidence="2">
        <location>
            <begin position="469"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="422"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="253"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="536" mass="58195" checksum="D8D25C934FB5ACC4" modified="2017-01-18" version="1">MSDEDSFVAGQDPSVDRNEKEIINVESSSPYRGKSENHILNELKQSDAKVVTKTELPQLPINKRQHVAVGNNQTNVLGTPSSKKQKVSSPVEAPLAHAVITSIAEQLALNRKNAPSLPTKHNPDSSVSKSNVKIKKSRENTPDTVSFNQKXLAVTPTSSEPIDPTAKTTPKSLLPKSVSKKPIVKLKTSDEKAPKTVKPKIKKAPSSDAKQTQNSLDPVAKVKKXPTKKVQKDSKPTKQVKVQPLTTKTNTIPSLAKSSSTTSLSKPKTPKKTASTSSKVSASTSKINNNTTTKPANPLEPGKTPSKKPNTQISSLAAPQFSAKKTSTGLVDPPEELASPQVLAAPQPSNQAQKLTTCHSVIGSKPNNGNGIVVVDIPLYNSESNDYLDENGQVVFNYASMINEYNRKNKIPVKKNLLGELENNDDMEDMEVEELDDEVDDEDDGDNSDDLDDANAGTSKQDPSKKPSTTKGKSRIGKYDIEDPFIDDTELLWETQRAATKDGFFVFFGPLIEKGHYASFERIDGTMKKGGIRSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFM8</accession>
    <name>A0A1E5RFM8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mitotic check point protein BFA1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFM8"/>
    <dbReference type="STRING" id="56408.A0A1E5RFM8"/>
    <dbReference type="InParanoid" id="A0A1E5RFM8"/>
    <dbReference type="OrthoDB" id="19159at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990334">
        <property type="term" value="C:Bfa1-Bub2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034586">
        <property type="entry name" value="Bfa1/Byr4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35140">
        <property type="entry name" value="MITOTIC CHECK POINT PROTEIN BFA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35140:SF1">
        <property type="entry name" value="MITOTIC CHECK POINT PROTEIN BFA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="463"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="721" mass="81856" checksum="C03BA66954EFEFAF" modified="2017-01-18" version="1">MPLKPALHDLGISTFEETDLSTQNVYQPVMKSSMLPKSASVNFQITKKNDESVGYQSLKTNNITPPQRVKGSTGNRKNYNISPDLLKPFSKPNSLAPPPPSVSSAGSTVFSNTKKYPTSYSESEKGEFDDADHAEMSFRKLKAKHQNTPTLNVNPQTRQFLDDFEFETDEENTDTNETDEEEYINAKAAQSHPPSRSKKYLNKMDVELNDIQENFEKKFHIFPEVPQVKPSSYKMKNFQQEQQNQAPTIHAQNSTHHNPLLFQPKSMMSLRPTSSKGFLSSPDKLATTYEDQTYWPTPFDKLGSIREVRRKASRTTLPKYKSMSNLKINAISGGSGNSGMTTTRKLQNKQSFPSLKVGNNNHVFSNERDLHQFQDKNDNFRDDTFGEDFDDMVPQYLVSSSPKKSIRPQAINVLNNFDEVEQVGRGINGDYERDYPELSTPQLHKPVAKRRWKSKKHCLNSFRENSDVEEEDSSDTGTVYRTGMRKPSNIQLLKQEIDSNTPITKGKMIYNPETFKWEGNTDALSEFPENYKYKPANRRSSVVKNDGIKLRKSRSTLFSKSTGNKIEATSTGSGLQSRKVVGNMQFDSKNQRWVSLHGDSGEIDPFKNIEDHVLAQSSSYGNSRFPSSGTDSNLKRYVSTSSANGNARASSTGKMYSTSRPQFNPTFVISSMKLEVFYHEENKWLDKLGGWINQDLEHADSDTRFEYAYEIRNMVLSSTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFM9</accession>
    <name>A0A1E5RFM9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RFM9"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="292" mass="33108" checksum="3429778F6D4C92DC" modified="2017-01-18" version="1">MNANRQTENLKKIYDLIDKLKLLMRENRVKRTNIMKELDNVALISNRLPAGAASTDNDTSTSTTDIPTKTTNTINAATDXGNDSASSFSAEFNSTPEQPQFKHAFSQGDFLPVNVINILIQKYGSSLDAETVRTVQILQSQLGTLPMTPLNSTAFISTASGTTYDTEGQMYENRTLLLKAQNRLLHEINDLLQRDNGELISFLVNQYETRIQDELIEKLRYSIYVNNMNFLKFFANQYTHNIISFEVTSINRYMQGIEDYDELLRVSTALRNLYGFVHEYGLDLQTSEETAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFR2</accession>
    <name>A0A1E5RFR2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ER membrane protein complex subunit 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFR2"/>
    <dbReference type="FunCoup" id="A0A1E5RFR2">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFR2"/>
    <dbReference type="InParanoid" id="A0A1E5RFR2"/>
    <dbReference type="OrthoDB" id="1894652at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21203">
        <property type="entry name" value="ECM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184731" description="ER membrane protein complex subunit 10" evidence="1">
        <location>
            <begin position="20"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="209" mass="23445" checksum="0759AE7ABC248973" modified="2017-01-18" version="1">MKLSALIYASLAGFQVVSASSMLITARNVQSQEEHSFFELYKDDESGKYTVENLNAELPVGSNELYCFTASGDIGGLENNKDCFTVAKLVSPLSYDLFIDEDVGRLTLKYSNKSLNEITPITRSAVQGFSAGLTKLKKVTKQYPKNQKNVKNPYEQEGVIEDKAIEAEKEVEDEKQLTFFEKNWRFMIAGLLIYSVLFRKSGPKPAKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGP7</accession>
    <name>A0A1E5RGP7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Monoglyceride lipase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1368</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGP7">
        <property type="interactions" value="402"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGP7"/>
    <dbReference type="InParanoid" id="A0A1E5RGP7"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051044">
        <property type="entry name" value="MAG_DAG_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11614">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="2">
        <location>
            <begin position="100"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="377" mass="42413" checksum="608B87F07E40155A" modified="2017-01-18" version="1">MPKFFPKLDPEYAKNYSPKPFPFDIPADKIPPVQKHQYNGANFTYMIWPATATTNTNTNTNTNTNTNTNTNTSPPKNNLIDNENSATNGIGHSDCENNTTRIIFVHGFGEYTKIYYRLMYDLSLHGIESCFFDQRGAGETSPYKLKGLTDSKHQFDDLDHFIEFFTTQPETKPTKTYLFGHNMGGGIVLNYGFLSEMKNLHKIDGIICTGPLIKLHPRTRPGFVLTQAMKLLANCAPTFQVDGDLDIDGITDDLKVQQWMLNDFPWTTPCVGSVRLLYDMLYDGERLVEESGRGSKFVTSTSCATFPAKLLILHGLKDTINDYKASQQFMEQILPSVDPQNRISKKLVLLPQGXHSLALCTGNGVYDDLINHVLEFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGQ4</accession>
    <name>A0A1E5RGQ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Zinc finger protein ZPR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1097</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Acts as a protein folding chaperone for elongation factor 1-alpha.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZPR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGQ4">
        <property type="interactions" value="1010"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGQ4"/>
    <dbReference type="InParanoid" id="A0A1E5RGQ4"/>
    <dbReference type="OrthoDB" id="308464at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.420:FF:000001">
        <property type="entry name" value="Zinc finger protein ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.420:FF:000002">
        <property type="entry name" value="Zinc finger protein ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1040:FF:000001">
        <property type="entry name" value="Zinc finger protein ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1040">
        <property type="entry name" value="ZPR1, A/B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.420">
        <property type="entry name" value="ZPR1, zinc finger domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004457">
        <property type="entry name" value="Znf_ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040141">
        <property type="entry name" value="ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042451">
        <property type="entry name" value="ZPR1_A/B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056180">
        <property type="entry name" value="ZPR1_jr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042452">
        <property type="entry name" value="ZPR1_Znf1/2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00310">
        <property type="entry name" value="ZPR1_znf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876">
        <property type="entry name" value="ZINC FINGER PROTEIN ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22794">
        <property type="entry name" value="jr-ZPR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03367">
        <property type="entry name" value="Zn_ribbon_ZPR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00709">
        <property type="entry name" value="Zpr1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger ZPR1-type" evidence="9">
        <location>
            <begin position="58"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger ZPR1-type" evidence="9">
        <location>
            <begin position="319"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="513" mass="57240" checksum="5AF2CAAC84B77115" modified="2017-01-18" version="1">MNSHKKVKLDDMSEKKNDIFTPVGEAAANATATATXTGIAXATEEADNQNRSATEVQSLCMHCHSPNGVTKLLLTSIPYFREVVLMSFECPDCGFHNNEIQPASEIQERGSQYVFKISSKEDLNRQVVKSEKATCKFLELDIEIPPQRGQLTTVEGLLQEMLSDLEAGQEQRIADGQQQVHDQIAAFVGKVRTTLDTVGETQSETPDASTTTTTTTTLTFVLDDPSGNSWIEYKPDDGKKWLHKEYNRTDEQNVAIGIMTQDQLEQKKLLVNAIAPYADQIASTTESNNTTNDAKKSQSFLSDATDVENYNNEVQTFKGTCPGCGVNECETHMKPVNIPHFKEVIIMSTVCDHCGYKSNEVRTGGEIPVKGKKITLFCDEPVEDLKRDILKSETCSMYIPELHLDIQEGTLGGRFTTLEGILKQINDELYSRVFTTTSDSMDTETRKNWETFFGNLGKAINGELKFTVILVDPLAGSYIQNVYAPDEDPNMKYEDYERTQEQNEDLGLNDIDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGR4</accession>
    <name>A0A1E5RGR4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">L-lactate dehydrogenase (cytochrome)</fullName>
            <ecNumber evidence="19">1.1.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Cytochrome b2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Flavocytochrome b2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">L-lactate ferricytochrome c oxidoreductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">AWRI3579_g1231</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(S)-lactate + 2 Fe(III)-[cytochrome c] = 2 Fe(II)-[cytochrome c] + pyruvate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:19909"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16651"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.1.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:19910"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">In the C-terminal section; belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">In the N-terminal section; belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.2.3" evidence="19"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGR4">
        <property type="interactions" value="341"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGR4"/>
    <dbReference type="InParanoid" id="A0A1E5RGR4"/>
    <dbReference type="OrthoDB" id="1925334at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004460">
        <property type="term" value="F:L-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006089">
        <property type="term" value="P:lactate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02922">
        <property type="entry name" value="FCB2_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000009">
        <property type="entry name" value="Cytochrome b2, mitochondrial, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000062">
        <property type="entry name" value="Cytochrome b2, mitochondrial, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008259">
        <property type="entry name" value="FMN_hydac_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037458">
        <property type="entry name" value="L-MDH/L-LDH_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF148">
        <property type="entry name" value="L-LACTATE DEHYDROGENASE (CYTOCHROME)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00557">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="24">
        <location>
            <begin position="93"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="25">
        <location>
            <begin position="202"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00052399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00061137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00061589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00066458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00068515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00075949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00078774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00078938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="OEJ86098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="607" mass="67063" checksum="9806B24113C0FF5D" modified="2017-01-18" version="1">MFATKLVGKKIPSSLLKVQQSKNLTRQFQLAFAKRNLNTVTKTQKNSHSSSSNFAKKILYTGSVAAAALLSFNQLTASNNDNVLDNQSPKKDLPKVSVDEFIKHDRPKDCWVVINGTVYDLTRFIDVHPGGPNIIEQNCGKDVSKIFNPLHAPDVIQKYIDPKDIKGTLDGELPESHIAQPLSPGETADDVARKAALRNNLPDLESLITLYDFEYLASKILSKQAWCYYSSAAEDEITHRENHNAYHRIFFKPRVLXDVVEANTSTEMLGEKQDVPFYVSATALCKLGNPEEGEKDIARGCGMGDFKMTQMISTLASCSLKEVSEAKTNPDQVQWFQLYVNSDRKITDNLLKEVEKLGMKAIFVTVDAPSLGRREKDMQVKFADSSNPQIMNSNDKTTAPSKGASRSLSTFIDASLSWKDVQDIKSKTKLPLVIKGIQTKEDALKAAEIGVNGIVLSNHGGRQLDCSRAPIEILAEVMPALKEKGVDTKKDFQVFVDGGVRRGTDVLKALCMGATGVGLGRPALYANSTYGKDGINKLKHMLQEEVEMSMRLLGVTSIDQLDESYIDTSSLNLRSVTVPRDNLYSQVYEPRTHATFQNGADSDDSDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGU9</accession>
    <name>A0A1E5RGU9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Adaptin medium chain APM2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1311</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGU9">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGU9"/>
    <dbReference type="InParanoid" id="A0A1E5RGU9"/>
    <dbReference type="OrthoDB" id="10259133at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018240">
        <property type="entry name" value="Clathrin_mu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00991">
        <property type="entry name" value="CLAT_ADAPTOR_M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="7">
        <location>
            <begin position="284"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="178"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="178"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="195"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="609" mass="70685" checksum="7B8550DF13D1FC79" modified="2017-01-18" version="1">MRLPVLIHDDIIDDFKNIYCDVKKTSIIEAAVPIVRSSSYEFVHYAYIVRSQLIYLAIIVPFIEDIRFGNDCNSTKIGNTKSSCESVHASYDMKTVNVMTSLVYLDNLHQLIAKYFNNTNVTPMLIANNYNLLDALLDESIDFGVPQLTNYNVMQEYIKIKPVLNDFLMEDVTEFFSDNDNDNDNDNDNAEDQLDAQSNKDTSSFVEKERKQKNIGWLNSGKELLSLSHKSRKSKNKSKFQSYWGGKEGSXETLGQDETYMNSFLARTTTSAINWRPHGIYYAKNEFFLDVVEKLQYYQDLETGEVRENFLTGIIFCKSYLSGMPELKVGISDNKLKLNNEALLKKNFRFHHCVQLEDLDDNCEIKFIPPDGEFELANYEFKRRRNDMDPPIIKIVKYEIKEKPLKHVRIFVDFTTYFRKQSSTTKLKIQIPILNLFSEHKVDLTKNPKFKTDWGKLYFNISDDFVLWDIGDIKGGYGDKTYGCVIQFDLINEEALKKEQELLKNSMDPPPLRQGPKLEVIYQQLHGETGKQNESKKTDDAGKAQLNSLIESRKNNLIKVEFEIPYYTSSGLKIDFLKIHEKSLANYQSFPWIRYKTTNDDAYCFQTPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGZ6</accession>
    <name>A0A1E5RGZ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1434</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGZ6">
        <property type="interactions" value="310"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGZ6"/>
    <dbReference type="InParanoid" id="A0A1E5RGZ6"/>
    <dbReference type="OrthoDB" id="10264220at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026028">
        <property type="entry name" value="V-type_ATPase_116kDa_su_euka"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF59">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A, GOLGI ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001293">
        <property type="entry name" value="ATP6V0A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="538"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="583"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="671"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="703"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="764"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="864"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="895"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="11">
        <location>
            <position position="709"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ86178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="956" mass="109665" checksum="5B307895B89E672D" modified="2017-01-18" version="1">MRGLHSTQSSSYLIHNEEAVFRSATMSYVEIYFPLEISRELTYLVGELNCLMVKDLNENVSKFQRSYINQIKKLTEVESHLQYMSEIVQLHFDQTIKYLPPPELLFDDDGNNFEDERLNSQGQLLDADSFSNSDTNEQEDSHPTTSVHRTFLQNFRVPNSEEIHNCCEDISQFSSRLRSLNDSYLEMIKKTKRLLEHRSVVYESSKFLKFNPGXIVSEASAGLPQNFNNPNDLDLEVESIRDLRNENFDISDDDFDIDSEHDFVNHDXQSAHSNAFMSENILSHYGSLKIGSIERTKIEPLRKLLWRTSRGNMFFHNIPIDPTINNKVHDLNSTSASFVQKSQFNPNTDNPEGEVEKDCFVVFSHGELLMERITKIIDSLNGTVYDLPDGATSSTLNMQIEQVQQILDATEQALLTELVVVQDQLLKWTRLVSKEKMVYSTMNLFKKEHHDGAGLILEGWIPTKRLDRFTNKLKDFTHLNYPDMVQPVVSLIDTNKTPPTFFETNKFTSAFQSIVDAYGVASYKEINPGLATIVTFPFMFSIMFGDLGHGIIVMLCGLYLIINETQFTKKQRSDKIKGQQNEIFDMVFGGRYVILLMGLFSMYIGLLYNDIFSKSMTLFGRSGWEWPKDFKIGETIEATKVQGKVYPFGLDFGWHGAENGLLFSNSYKMKLSILMGFIHMTYSYMFSYVNYKFKNSKVDIIGNFIPGLLFMQSIFGYLSICIVYKWGKDWIKDSKEAPGLLNMLINMFLSPGNIDVPLYPGQPFVQIVLLLAALVCVPWLLLYKPLKLRKENANAQNRGFNSTEHEFLENRLLESDELAGEEMRVTDYEHPEEFNFGDIMIHQVIHTIEFCLNCISHTASYLRLWALSLAHAQLSTVLWTMTIQNSFSSANPGSFLSVLKVVLLFGLWFVLTVCILVLMEGTSAMLHALRLHWVEAMSKFFEGEGYAYEPFSINEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHC5</accession>
    <name>A0A1E5RHC5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NADPH dehydrogenase 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g997</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHC5"/>
    <dbReference type="STRING" id="56408.A0A1E5RHC5"/>
    <dbReference type="InParanoid" id="A0A1E5RHC5"/>
    <dbReference type="OrthoDB" id="3969599at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045247">
        <property type="entry name" value="Oye-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893">
        <property type="entry name" value="NADH OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893:SF91">
        <property type="entry name" value="NADPH DEHYDROGENASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="4">
        <location>
            <begin position="18"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ86300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="152" mass="17421" checksum="E948E31D5F95BE39" modified="2017-01-18" version="1">MLQRHKVLKNTCALSIKKTLMDKLHAHFIGELEKRAKENPENRLAYVHIVEPRVPSPAFGEGEHEYLEGTNDFVFSIWKGIVIRAGDYALNNTEALKDVNENDRTLLAYGRFFISNPDLVDRLEKGLPLNAYDRDTFYTPGGKGYIDYPNYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHJ6</accession>
    <name>A0A1E5RHJ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinetochore protein SPC25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1593</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHJ6"/>
    <dbReference type="InParanoid" id="A0A1E5RHJ6"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="52"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="226" mass="26085" checksum="66F33E8AA07AEEFA" modified="2017-01-18" version="1">MKSSNTQLNSFALLQGEMSRLEERVLHNYLPVQLQFTQALSQEHQAACDLLLAEHEQRLADIQLLNKQYDELKQNIETQRLQKLKKLGLLDSLKLKLQTYTTKHQQLKQELVKKNEVYASLTNDIQNLNTTINFQEIKDLNEVELLEAILGFKIQAYADESHAVKFVFDPNGYITINTKENLIVDIQCLKVSHGKAAITKNELQVLLANNNYKEFIIESRKYVLGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHN0</accession>
    <name>A0A1E5RHN0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA repair protein RAD50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">AWRI3579_g1555</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SMC family. RAD50 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHN0">
        <property type="interactions" value="1044"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHN0"/>
    <dbReference type="InParanoid" id="A0A1E5RHN0"/>
    <dbReference type="OrthoDB" id="18797at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030870">
        <property type="term" value="C:Mre11 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003691">
        <property type="term" value="F:double-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051880">
        <property type="term" value="F:G-quadruplex DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043047">
        <property type="term" value="F:single-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070192">
        <property type="term" value="P:chromosome organization involved in meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007127">
        <property type="term" value="P:meiosis I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000593">
        <property type="entry name" value="DNA repair protein RAD50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001195">
        <property type="entry name" value="DNA repair protein rad50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18867:SF12">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18867">
        <property type="entry name" value="RAD50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="14 19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0469">Meiosis</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Rad50/SbcC-type AAA" evidence="20">
        <location>
            <begin position="6"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="219"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="753"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="1045"/>
            <end position="1093"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OEJ86384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1313" mass="151837" checksum="CA64E057FF3CE87E" modified="2017-01-18" version="1">MSSIDKLSIQGIRSFSPEAKQEIYFGTPLTLIVGANGCGKTTIIECLKYSTTGDMPPNSKGGAFVFDPKMKGSREVSAQVRLFLRATDQRKYSITRNLQTILKGKTQSFKTLESDIGRLENDGTVTTMSSRAQNLDERVPKFLGVSKAILEYVIFTHQEDSLWPLSEPAVLKKRFDEIFQVFKFSKSVDLLKSFKKESVADCKILQETVNYLKTDRNRSQSTKLKISQIQKKINQYEESVTSLKVELDDIIAQQDKLFKSNQEYQQVLSKLETLENKKNLMISQMTTLKQNLTNTIENKSIQKLQDDLQNFEAILLSKEQEIEDYKKNHLEPKLQELEMNRKLYNELIGKQGEYNQMIKEYELNKEKLQEMEIMFKADFHLEPNTEIISSHLKRLKTSYQVFKNTNESELSFLQEKANELGTQSKVEMGNLNFINNDIFRTTNALKVLEKDLQKITKNPLNDEKRQADIQKSFDDFKQQLVDFEESETIKKISQQIDNEFEQISHLETSLENIQGKIMASKKQSDLLINYNLVKQDLQKAQGSLAKSLFDFVDQNKKMDNVLDLADIDKFKPTLEHCKEVVSIYENSMDDAEILSASSNQKYRGLENEHGNSIRAHKAKVDEIRDTENQLAKLKSFIDPVLPIKEINKYSDQVSELEETEKELLEGIKAREVFGQFFQKSKDIAEVSKCCFLCERTFKNDEQGFDKFIARLNSKLKIDVEDFVQEKTYVEQKLANLKKLAPSIQTFNEKQHFLVILKKDLTNLEGNLQNSEKELGKSKTEYDRAVSFYDRLKKLRTFVLAIERLIATVESQTFKLNELNRDVELKESNTENYESLDALNTKQSTMNNELKQMRKNLYELQTTKENQTRTYNKLQQYVKDQGFTLAEIETMNLEKNKISNDIAEQKKTLENFAVSKKMNEETVSRLQKSIQTTNQALVELKDKYQTEGDTLQKNLDEFTRISDLFQNLNDQTKTFEQNHQKKSIEVSKQVETADLSIKQLDSEIVSINQHINAELAKVNDSKTTKNNIRFNIEYQELNISLAKVTKEISEMDVQNAQVRRSEYEKQALELKHMYEQVSSENASKVGEIKQLRNQIAEMTHLLNTEYKGIDQKYMESLASLQSKTLKNADLDEYSKLIEMSIIKYHATKMKEINRVLDELWRSTYVGSDITTIMIKTEQVTSARGRSYNYKVVMFKDGYELDMRGRCSAGQKVLASILIRLALSESFGSDCGVIALDEPTTNLDEDNIASLAQGLHNIIQARQHQRNFQIIIITHDEKFLRYMKASDFADGYFKVDRDSLSTSTISWIDIKNIYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHP5</accession>
    <name>A0A1E5RHP5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cell membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1242</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHP5"/>
    <dbReference type="FunCoup" id="A0A1E5RHP5">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHP5"/>
    <dbReference type="OrthoDB" id="4068213at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009571">
        <property type="entry name" value="SUR7/Rim9-like_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052413">
        <property type="entry name" value="SUR7_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28019">
        <property type="entry name" value="CELL MEMBRANE PROTEIN YLR413W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28019:SF2">
        <property type="entry name" value="CELL MEMBRANE PROTEIN YLR413W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06687">
        <property type="entry name" value="SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="530"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="565"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="610"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="636"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="640"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="664" mass="70980" checksum="BF3733AF27AB126B" modified="2017-01-18" version="1">MKACALFYPIVALFTLAIFLFNIFNIAGSASGRLSNVYMGEATIRHINVTKILPNVAPIVNVFAQGLTAPNTNTTQIFDSMLALGQSAALKPVLGLMASSEDLPATMDALYTLTPIAASASNNKLSTEMAELIQNTNNQTLLLESLISLSGSNTTATTAALVPVMKLLQNTNNATYTLEILASLSTASEEIPSAELKSILDLLGDSTNITATLGDFQILSTGSSAINTTVEAEIFNAIQTSTNLTQTFAGLTMAATSEEKPFITALADLLTQSSNSTATLIDLSTILTAQQAQQASNSSAALQASAASLKSLSALLASSTNSTLTLQTLPTLLSTSSSDSATQLKNLAGVLENSKNQTLSLQIISDLESSVSASTLSTVIPAFVKLLAASTNVNTTLTDLATVSAITQSNATSLTPLIAIMNAYENSANVSEEYIYQNIMPSMFDKMHLATDFRLGILSLCRYSSEGVKYGCTKAHSVQSFYMKDILYNELETSYFSPYVKALNLTKDDVIIVGDLVKDQKYYVPAVRTLLAFSIISFILAIFIFFGTCASMVKSVPMFDKINAFVSAPCLFISSLISSIILGALCHIIKKGLKHDNYNVSFKYGSAFWGLSWTGFVLAFFVAVFAFAKQIRHPKKSAADEEQFQEESTGESSEDFSVKKEISA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHX0</accession>
    <name>A0A1E5RHX0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mitotic check point protein BUB2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1431</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHX0"/>
    <dbReference type="FunCoup" id="A0A1E5RHX0">
        <property type="interactions" value="222"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHX0"/>
    <dbReference type="InParanoid" id="A0A1E5RHX0"/>
    <dbReference type="OrthoDB" id="10263206at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031030">
        <property type="term" value="P:negative regulation of septation initiation signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000026">
        <property type="entry name" value="Mitotic check point protein (Bub2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF263">
        <property type="entry name" value="MITOTIC CHECK POINT PROTEIN BUB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="36"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="336" mass="37981" checksum="EF05E1E3E1C004AC" modified="2017-01-18" version="1">MSQYESFIYNQQPVILNSSLSQLRYLILSQGLPTGKNNNRLRIHVWSVLLQCSMENCTSQYLALLSKGEDLPPLIYNKIKNDTPRTLVKDSTFQKTVSEDALLRGLCCFAWESIDIAATAAAAGLAEGDEDTHLSSFASSSGIHEDEKEALAEENVYKISEYVQGMNVLLAPLLFVCESSEPMAFRFFTKICRRYIPTYLNKNLSGVYKGAQLLEICLSIIDPKLSKFLRQDHLLTAEIYGIPSIMTLSACTPPLNEVLKLWDFMFAYGFHMNILFIVAQLVLMRSELLKSPTPMNLLKNFPQFNADEVIRLGVGFIAKLPHEVYQSLVEHLYRIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIA2</accession>
    <name>A0A1E5RIA2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial genome maintenance protein MGM101</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1161</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
            <location evidence="1">Mitochondrion nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MGM101 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RIA2">
        <property type="interactions" value="299"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RIA2"/>
    <dbReference type="InParanoid" id="A0A1E5RIA2"/>
    <dbReference type="OrthoDB" id="17164at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000262">
        <property type="term" value="C:mitochondrial chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009446">
        <property type="entry name" value="Mgm101"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31404">
        <property type="entry name" value="MITOCHONDRIAL GENOME MAINTENANCE PROTEIN MGM101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31404:SF0">
        <property type="entry name" value="MITOCHONDRIAL GENOME MAINTENANCE PROTEIN MGM101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06420">
        <property type="entry name" value="Mgm101p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1135">Mitochondrion nucleoid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ86638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="288" mass="32367" checksum="DBBAEA20AD6648A1" modified="2017-01-18" version="1">MSMXNIIFKRTYALKSAGATYTKTVKPAFPKSAPVYSKYASKATPTADTTTPDTQESTKANLDSAKQNWKYVSTKPAKSSIAESYPKGKTLENSLKGTTLADMQQWEASYSDNIPFNIDWSTSFFGLGAKPFSDQVQQRLAQALTPLDIEIKPDGLIYLPEIKYRRALNNAFGAGGWGLVPRSETIITDRLVTREYGLVCHGQLVGVARGEQDYFSPEGIPTATEGCKSNSLMRCCKDLGIGSELWDPVFIKTFKKQYCEEKFVEHVVNKKKKKIWIRKDRDIEYPWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIA6</accession>
    <name>A0A1E5RIA6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uracil catabolism protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1641</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RIA6"/>
    <dbReference type="FunCoup" id="A0A1E5RIA6">
        <property type="interactions" value="246"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RIA6"/>
    <dbReference type="InParanoid" id="A0A1E5RIA6"/>
    <dbReference type="OrthoDB" id="2595934at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001080">
        <property type="term" value="P:nitrogen catabolite activation of transcription from RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050797">
        <property type="entry name" value="Carb_Metab_Trans_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668">
        <property type="entry name" value="GLUCOSE TRANSPORT TRANSCRIPTION REGULATOR RGT1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668:SF9">
        <property type="entry name" value="URACIL CATABOLISM PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="163"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="272"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="787"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="28"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="81"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="278"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="787"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="812"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="830"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="842"/>
            <end position="853"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="898" mass="100948" checksum="FFED5A2402ACC2B0" modified="2017-01-18" version="1">MSGPAREIVHKENLPENSTANPTLVPISNVYSSSPSITSSQSNIRGSVKISSFLHPDEDGGDEKQANTIKEPALVKEKDQANVSTGAQDCSATAQKSTTSPEASSMNNTTTDFNMQSAEKTQDPSKHADNSTTPAAKTGQEEPSNKDTGKATKKPRKRRKTYSCESCRNLKTRCDFQPFLGKCHRCVVLGLSCSLTSESNVPAKNPAAKDQDNAHQLLTQGIPVMPSSITSLSERINTMEEKLKSVDDKLSVILSLFQHNIDGVSNEVFDNANNYSSEGDDNEYSADGDENNNSSLTGDDVAKTKNRKKRSFGQIEFPQSQLCTAHGVISKDPPFQLLDDIDKSLFSTKANNEKDAIAKSQRPFVVARSKFMEFFIKHEQLCYELSREFLVKAHFWIIPGGRKKIDREYVENHAFITSVLTIIAMGFDENNKYEKEQEELYPIVETLLSNTLTMFGKLIDHDIEAILYCCMFSISRKSQRYRQLKMNSLILCNFALNSLLTIISFHKIKEKVSSESYDAEDLFHLRILNSLTACKLEYSIGYGNYTIQDEQLKEFNDLTAKFPQTNFGDEIKLSEINLGDIVNAIFLNFKDYSTNCVESFEKKPTKILTFKELQYWLKNWHELSCKDGGGVLLFTFDFYHILICRTMLKEFCDRETFETHTLFFKTCLKTISDYCFSLLHGFLKLPPSLIKGAPSITLHQAVYSCLTLCDYLHCFDVQKKQKILNTCTKIYWHLNTIGEQLNEATQNVGMIIKNLIDTGKAKLSTNSENLSDEQLFSRIQQGSVHSSFSKNSVKTKTKTVSSNSKQARVKSLESSARNDSKQISPMRASPTYSESLYSNEKTSAGGTPGSTGALSQSNTASLLNFPDVEQFQSFEDFFQDFFYNLKPTSQNMFSSLDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIQ6</accession>
    <name>A0A1E5RIQ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Saccharolysin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1656</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase M3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RIQ6"/>
    <dbReference type="FunCoup" id="A0A1E5RIQ6">
        <property type="interactions" value="759"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RIQ6"/>
    <dbReference type="InParanoid" id="A0A1E5RIQ6"/>
    <dbReference type="OrthoDB" id="534666at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06455">
        <property type="entry name" value="M3A_TOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000074">
        <property type="entry name" value="Metalloprotease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.40">
        <property type="entry name" value="Endopeptidase. Chain P, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.10">
        <property type="entry name" value="Neurolysin, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024077">
        <property type="entry name" value="Neurolysin/TOP_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024080">
        <property type="entry name" value="Neurolysin/TOP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804:SF84">
        <property type="entry name" value="SACCHAROLYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M3A/M3B catalytic" evidence="8">
        <location>
            <begin position="227"/>
            <end position="710"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="714" mass="82795" checksum="137F7A7290F5A930" modified="2017-01-18" version="1">MTKPSECIISWDFPVSTVIQSTKSIIEKSNALYDRLEALPDPTMENFVLPLLQFENENDLLVNQLTFLKDVSSDEELRRVSTESARLFQEHSIETMLRRQLFAQLDTLMKKFTETKELQKENYDRDIYHFLEKVYEDFVRAGMKLSTEDQNKMAAIHKRISDQTLAFAQNLGEQKEFILFTKEELDGLSDEIMDQFEKFEEELESPTGKEIVEKFKVTFKYPDILPLLKNAKNQNTRKKAFVGDQNKVSALNEPLLLETLELRKQLGELMGYETYADYNLDIKMAKTKKTVFLFLKDLISKLKPAGEEELAVLKNLKKTDYEDQGLEYSPEEPYYVWDHSYYNNKMLKEQYDVDYEKLSHYFPLDQVLTGMLEIYESLFGLQFSEETDPELKHVWHEDVKQLGVWKLVGEHKEFIGWLYLDLHPRDGKYGHAANFQIASAYHDLNDSIDFKPIKHYAATALVCNFSKPQRKTGKPSLLKHDEIVTLFHECGHGIHHLCAGTSNVTRFNSPGSVEWDFVEAPSQMLEFFTWEPFFLRKLSKHYETGEQVPLEMIEKLIKTKHVNSSLYYLRQLQLGTFDMEIHTETNKNFQDSNDFDISKLWNNLRTNISLLSCGEGSQYTPTKGYNSFGHIMSSAYSAGYYGYLWAEVYAADMYYSQFAPAGTDLIEIGKIGSRYRDTVLGRSGVYGVTSSVTEFLQRPANNKAFLQELGIKDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RMX3</accession>
    <name>A0A1E5RMX3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL11m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g638</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RMX3"/>
    <dbReference type="FunCoup" id="A0A1E5RMX3">
        <property type="interactions" value="675"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RMX3"/>
    <dbReference type="InParanoid" id="A0A1E5RMX3"/>
    <dbReference type="OrthoDB" id="1091498at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.250:FF:000005">
        <property type="entry name" value="Mitochondrial 54S ribosomal protein YmL19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1550.10:FF:000004">
        <property type="entry name" value="Mitochondrial 54S ribosomal protein YmL19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="entry name" value="Ribosomal protein L11/L12, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_uL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006519">
        <property type="entry name" value="Ribosomal_uL11_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_uL11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_uL11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_uL11_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01632">
        <property type="entry name" value="L11_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="Ribosomal L11/L12e N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8 11" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL11 N-terminal" evidence="10">
        <location>
            <begin position="5"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL11 C-terminal" evidence="9">
        <location>
            <begin position="68"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ88247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="146" mass="15535" checksum="95B58EA01927B2AD" modified="2017-01-18" version="1">MNSIVKLIVGAGKAAPSPPVGPALGSKGIKAIDFCKEFNARTANYAVDVPIPCLITIKPDRSFQFEIKSPPTGYLLLKALGNPAGKGSAQANKFDIVPPVGELSLKHIYEIAKIKKTDDRHKDLEMKSIVKTVLGVAKSMGVKVVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN95</accession>
    <name>A0A1E5RN95_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Stress response protein NST1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g679</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RN95">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN95"/>
    <dbReference type="InParanoid" id="A0A1E5RN95"/>
    <dbReference type="OrthoDB" id="21629at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="126"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="227"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="497"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="634"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="973"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1244"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1289"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="455"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="134"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="505"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="531"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="548"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="584"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="597"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="654"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="804"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="987"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1289"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1302"/>
            <end position="1322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1334" mass="148852" checksum="5F03C05195BFBCD0" modified="2017-01-18" version="1">MSKKKSRQSNTHNQTEPAQAAHGLKTVDGVIDVTLYKDDDRPYPTSRVIKRNSNGDVVVEEIFDDDPHPSYAEATEVKIADEAKAGPSRLYDSHWESLSPAEKKSILRIQKDEVFDVLKIFQKGENKNDDNAKTAPNSDSQANKNGDAGEKMSPAHSDSYTEHPQSVHTGTHSTHSCCNCCGRRNVSIEDDLEDIYNYLYIQEKMKQPDLSHVLFHLNLVKDINNKISSSEIPPSPAKQIEREDPGDVNFKQNTGPTLAKDSDNDIQPPPSYKSLTTRTNDNPDFASAPNTGETTEFDYNQVFGLYRKKLEEFAQQYISGHPEINKEVASKIAEFPQLEVLAKDLYENGGKAFLQMVNADAPLSSENETTFEASAFLSSSNEKETSPEQNSAELSFNQFASILSVLGNKVDQPDFGSHFLSGDAKNILKNLQKKIFVENSQVPNGNFTFEMNDEVRRKILEDENLENDLRKLNIELNGKQSLHQATEVDQAAKEAVGPVAFNGESEPEEAYFDEDEEENEKYGTHLGDSFHDEDDDEEYYESEDYESEHEGNEHEHDHEHYHHCHHQHRPDSSQSQHQHQQCHHHGDTCDVEHDAPQESEGEEGSIDEEKRIEEGKKLIQIAITKILQKKLVSSYKEKMAESSRLTLLEELEREEAKKKEKDEKKARKREKEKEKKKQQQLMREKELERKKIEEERRKKEAEEKELKRREEQRKKVEEIKKKKDEQLRKKIEEQRKREEEEAKQKKLKEELKQKREEQRKKRELEKQLEKERKLEEQQRKLEEQQKKLEETKAKDRQKQTSLEESSKAEKQKQEALKRANQPEDNKRQAAAFPYNLGSAHEAVIPSADEFLSFPTSSQSLSSVTNPVNAQTLHSAVVDNSSGHPGLNNSVAASVSSRNNSIYNTIDPQTCNSATLGAQSSILNGLYSSPHNAFASSSADDSSKKFENTFMSPVDGAPASRTSSSWFQDQANNLSNDVSPFLEPPKPLSSSIQEESLTGGSPQPNNETLYKKYLDGLAISGPAYGAGHLSPYSSFAAPGHPLSSAANHTNNGNVSFSNVGNSIWSDNQTPSLSNRASLSHSMNESQGFFASNNHGYLYGPPSGLDNSTSFTAQSDGTTPSGVNVSGNLSQAICQAYVSTMIEGNTQHLPVGTLYSVVSSTSAEQLNYSFFIKQLLLMCSTHNLQLLNDDKGTITHVWWDVNNSSNLSVLQPPSAQRFPSQQSAGQTPITMSSAYGSVNNMNSLASTPGLRSSVPPTEVGINGLGQTGLSRQSGFNTAAAHNVDNGHVYNNMSYNAHSSTNLPQNGSTSLSNQNSSSTTSAQQSTYPFGVNTSIWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNW5</accession>
    <name>A0A1E5RNW5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Meiosis-specific transcription factor NDT80</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g741</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNW5"/>
    <dbReference type="InParanoid" id="A0A1E5RNW5"/>
    <dbReference type="OrthoDB" id="2288358at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1390">
        <property type="entry name" value="NDT80 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052605">
        <property type="entry name" value="Fungal_trans_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024061">
        <property type="entry name" value="NDT80_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037141">
        <property type="entry name" value="NDT80_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35144">
        <property type="entry name" value="MEIOSIS-SPECIFIC TRANSCRIPTION FACTOR NDT80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35144:SF2">
        <property type="entry name" value="MEIOSIS-SPECIFIC TRANSCRIPTION FACTOR NDT80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05224">
        <property type="entry name" value="NDT80_PhoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51517">
        <property type="entry name" value="NDT80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NDT80" evidence="4">
        <location>
            <begin position="6"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="NDT80" evidence="2">
        <location>
            <begin position="6"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="358"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="439"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="747"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="374"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="439"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="750"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="765"/>
            <end position="781"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ88570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="781" mass="87391" checksum="A65EAFE4E2DE71EA" modified="2017-01-18" version="1">MAKKSGKKVNNNQNLANNEKLDNTAKRPNSQGRVPPRSFLLYKAGPVFANATKYHQIVTAQSHTKVNVEIMSRIDRGFDFEEGSWTGYKRNYFSLVAGFEVSNIESGTFLDENYCVEINGSFLPVEYFATKITAGAIVENVEMPLVQHTAKRTNGIQSAPQIQAVIPSSLPSPQIIRETSNIRSSAKVEKYASELYLDKSISAELLEKKESIISNYPKGNIQKFAKYERIQFRSSDQIKKNAQFNNFFTLFSILGVVIKGNVVSLAEQENGVVQPVYMEHDNCTFVPITSKATPPLMVRGRTPSSYHPKKIKALKQKKKEVNELLKTLKANPSGLHGMTTEKKNHDFDAMATSQKEFVASKKAKSKTVKDQQESDSTLKSSRKTNTMQKRKPLGMVQKKQPSRQVDGKIISSFEQMLQHTNTGETNDQSVGGLLKKNEALNQKEDPRNGGKLEQTESEGDLSLMKDESFLESNMPSDRPEAGLSSGRKVNLPVCATLSNTTKRVFVRRTRRAESEFYQNVFANDGFERKLRSARDVPSFTVQSVPSTGSRSFKQTFGDEFNGNEISVGEITQDSFTSPVKKINKVHKSVFGADEVSVESEGFENKSLEWNAFEKVEGSVFENHNAPLILLNGTKQTCTQNTEGSGMGRTQHTNLFKGTKFFANNFGKKLENRKNQEGNHSFQTQPVESNWRSLEPYKGSFDGMLPVSSSYSRVSSSISGFSSSRLYNDIKFQDVAESFLACKFNKGAPRSETSYKSSNITTNNEMENKEARKESDEHILNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP05</accession>
    <name>A0A1E5RP05_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Protein farnesyltransferase subunit beta</fullName>
            <shortName evidence="9">FTase-beta</shortName>
            <ecNumber evidence="2 9">2.5.1.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g930</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the transfer of a farnesyl moiety from farnesyl diphosphate to a cysteine at the fourth position from the C-terminus of several proteins. The beta subunit is responsible for peptide-binding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-cysteinyl-[protein] + (2E,6E)-farnesyl diphosphate = S-(2E,6E)-farnesyl-L-cysteinyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11535"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:86019"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the protein prenyltransferase subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.58" evidence="2 9"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP05"/>
    <dbReference type="FunCoup" id="A0A1E5RP05">
        <property type="interactions" value="721"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP05"/>
    <dbReference type="InParanoid" id="A0A1E5RP05"/>
    <dbReference type="OrthoDB" id="10261146at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005965">
        <property type="term" value="C:protein farnesyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004660">
        <property type="term" value="F:protein farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097354">
        <property type="term" value="P:prenylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02893">
        <property type="entry name" value="FTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026872">
        <property type="entry name" value="FTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF6">
        <property type="entry name" value="PROTEIN FARNESYLTRANSFERASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 9 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="10">
        <location>
            <begin position="93"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ88607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="469" mass="53023" checksum="28354A99711136C1" modified="2017-01-18" version="1">MESKTEKRAKYINSLLLGRKRDIKYEMSNTQPGLVNTEAEEYIDIDQGNDQSEKEDESVIPMIQELRTETTESKEETLTDILDCYNSNFLHDFNPQHHIPYLDFFLQHTLPPQMLALDASQPWMVYWLVNSLYIMQNGNIGDELRSRIKDKFIVQSSVSPFSALPRPASFGPFAGGIMQYPHLASNYAAILSLVLCEDEEVLSKIDKDYVYEWLMSLKLPDGSFETCEKCGETDTRGVYCALSIASLLGIMSEELVENSLDFLIKCQAYEGGFGGRPFEEAHGGYTFCALASLVIIIDYLHGAKKTLNNFIDVDMLLRWISARQDNEAFGLNGRSNKLVDGCYSFWIGGTGSILEAYGYTNPIDKEKLRAYILQCCQSDEFPGLRDKPGMRSDFYHTNYVLLGLCCVDSQFSLNNENHSTFSIEATLRNSKDIPGVAKINPIYGLPCSIIAKVYSWLSQEDKEKNENAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP11</accession>
    <name>A0A1E5RP11_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone chaperone RTT106</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Histone chaperone rtt106</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g613</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RTT106 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP11"/>
    <dbReference type="FunCoup" id="A0A1E5RP11">
        <property type="interactions" value="151"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP11"/>
    <dbReference type="InParanoid" id="A0A1E5RP11"/>
    <dbReference type="OrthoDB" id="75754at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050454">
        <property type="entry name" value="RTT106/SSRP1_HistChap/FACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040770">
        <property type="entry name" value="Rtt106_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849:SF3">
        <property type="entry name" value="HISTONE CHAPERONE RTT106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18469">
        <property type="entry name" value="PH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone chaperone RTT106/FACT complex subunit SPT16-like middle" evidence="13">
        <location>
            <begin position="206"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="337"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="355"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="425"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="445"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ88620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="474" mass="53672" checksum="FE224532E08D999C" modified="2017-01-18" version="1">MHSFLESLPKELQTELTSLLVNFQDGKERTGFLSVVEKIYNFAKDNSDDMNRKKPKIETKADNEAKTNIFTLNDVSAISPIRKKVDFSISYNANTKSPEIHLSKDNISQLCITNLMNNVTFATFLPVPEKSNLSYLLIQYQESINPSFSEPVLLSLNKDSIIRQLSGLKVVNTLPGNAHLQGNFQQCIDYIRKQAILTGFRIVDPFNHSTKAFTESSHAVQPFYINCHRTTKEGTLYFLADYIIFGFKKPILIFKTSDINSITYSSITRLTFSCTLIDSNGEKTEFSMIDQSDYNTIDNYVKSKEVINKSMTAELKAKPVGKPQANEGVQESALAAAEKQLAESGPSINDINMNLDEDDEEGDENFVGESDLSDGSGDSDDDSEHENPGSHSIDNEEEEEEQKESNKQFTHTKQNAQEEKMDHVEEPEELEDEEDQNPSRHEQDYGNEDMDEEEEEEEEEEEEEDEEDSGAEYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP30</accession>
    <name>A0A1E5RP30_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP30"/>
    <dbReference type="InParanoid" id="A0A1E5RP30"/>
    <dbReference type="OrthoDB" id="10647369at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="183" mass="21182" checksum="C7001C5AD0534956" modified="2017-01-18" version="1">MAQLNNNVDIIWPTENDRIIRTNQTLQTIRYLVKLGNFASLIVSIVYLAFRNILVPNYEKLLEQRSDFNRNTLFALRKTLARARKLMKDARASETPTDENQFSIFTNNRCKTYIERIKAVNLSIASESEEANSLQTAFQNFAENLHNMQYRHNKDKFDVLKKSNAIVADLRTLKGKIIKEGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP65</accession>
    <name>A0A1E5RP65_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nucleolar protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g812</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP65"/>
    <dbReference type="FunCoup" id="A0A1E5RP65">
        <property type="interactions" value="794"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP65"/>
    <dbReference type="InParanoid" id="A0A1E5RP65"/>
    <dbReference type="OrthoDB" id="3972801at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009383">
        <property type="term" value="F:rRNA (cytosine-C5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000192">
        <property type="entry name" value="NOP2p rRNA m5C methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1170:FF:000001">
        <property type="entry name" value="Ribosomal RNA methyltransferase Nop2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011023">
        <property type="entry name" value="Nop2p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023273">
        <property type="entry name" value="RCMT_NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="RsmB/NOL1/NOP2-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00446">
        <property type="entry name" value="nop2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF30">
        <property type="entry name" value="28S RRNA (CYTOSINE(4447)-C(5))-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02012">
        <property type="entry name" value="RCMTNOP2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 10 14" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 10 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="13">
        <location>
            <begin position="278"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="154"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="85"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="99"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="495"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="370"/>
            <end position="376"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="394"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="421"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="438"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00082314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ88493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="630" mass="71072" checksum="4FFEB054858BE6A7" modified="2017-01-18" version="1">MGSRRHKNKQRDPPTLEEFQAKKSYNKKDSKSKRVSSHDDKNKNDTKKRTRTEYTETSEAPKGTKKSKKVVEKDTAENEDDLPEVDLKELANAKKTLFDDSEEEEEEEEEEEVDQDLQDEFDLEQEYENESFDEDTGAGKKDNTWSDDEDEEAIEELNAANMEALSQKLDEEQALEDEEAEQELLEAGKLQPRATDILPTEEQQSLEEQMQPDLTAIRTRMIEVVKVLEDFKNMGDQSRSRSEYVDRLLKDICKYFGYTPFLAEKLFNLFSPAEAMEFFEANEIARPITIRTNTLKTRRRDLAQALVNRGVNLQPIGNWTKVGLQIFDSQVPVGATPEYLAGNYILQAASSFLPVVALDPQENERVLDMASAPGGKTTYISALMKNTGCVFANDANKARTKSLIANIHRLGCSNTIVCNYDAREFPKVIGGFDRVLLDAPCSGTGVIGKDQSVKVSRTEKDFMQIPHLQKQLLLSAIDSVDCNSKTGGVIVYSTCSVAVEEDEAVIDYALRKRPNVKLVDTGLQIGKEAFTSFRGKKFHPSVSLARRYYPHTYNVDGFFVAKFVKTGPSKFDDNKASAYEKEAAARQEALEEGIIHDDFASFEEDEDEKYITKAKKHGLLKKGVNPKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RPH3</accession>
    <name>A0A1E5RPH3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">RNA polymerase II subunit A C-terminal domain phosphatase SSU72</fullName>
            <shortName evidence="12">CTD phosphatase SSU72</shortName>
            <ecNumber evidence="4 12">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g591</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. SSU72 is required for 3'-end formation of snoRNAs.</text>
    </comment>
    <comment type="function">
        <text evidence="1 12">Processively dephosphorylates Ser-5 of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 15">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 15">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the cleavage and polyadenylation factor (CPF) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the SSU72 phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4 12"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RPH3"/>
    <dbReference type="FunCoup" id="A0A1E5RPH3">
        <property type="interactions" value="969"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RPH3"/>
    <dbReference type="InParanoid" id="A0A1E5RPH3"/>
    <dbReference type="OrthoDB" id="57957at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008420">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000039">
        <property type="entry name" value="RNA polymerase II subunit A C-terminal domain phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006811">
        <property type="entry name" value="RNA_pol_II_suA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20383">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT A C-TERMINAL DOMAIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04722">
        <property type="entry name" value="Ssu72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 12" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU369031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ88593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="220" mass="25157" checksum="7A1B1C6F8CB793BA" modified="2017-01-18" version="1">MSVKTESNTGSSTPDIQEQPYKSKLSFCTVCASNNNRSMEAHKVLSNAGYNVDSFGTGSAVRLPGLSIDKPNVYNFGTPYDQIYNDLKNQSEERYTRNGLLKMLDRNRKIKAAPEKWQLSQKNYFDFVFTCEERCFDSVIEDLMYRGGMKNKIVHVINVEIKDDNENAIIGAKGILALADKINELTLTEPVNYEDYLFEVFGEWQKQHPKLPMLYAVAYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV38</accession>
    <name>A0A1E5RV38_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Multisite-specific tRNA:(Cytosine-C(5))-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g475</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RV38">
        <property type="interactions" value="1318"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV38"/>
    <dbReference type="InParanoid" id="A0A1E5RV38"/>
    <dbReference type="OrthoDB" id="6093671at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016428">
        <property type="term" value="F:tRNA (cytidine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057285">
        <property type="entry name" value="Pre-PUA_NSUN2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057286">
        <property type="entry name" value="PUA_NSUN2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023270">
        <property type="entry name" value="RCMT_NCL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="RsmB/NOL1/NOP2-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22808">
        <property type="entry name" value="NCL1 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22808:SF1">
        <property type="entry name" value="RNA CYTOSINE-C(5)-METHYLTRANSFERASE NSUN2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25376">
        <property type="entry name" value="Pre-PUA_NSUN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25378">
        <property type="entry name" value="PUA_NSUN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02011">
        <property type="entry name" value="RCMTNCL1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 10 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="12">
        <location>
            <begin position="57"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="428"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="714"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="436"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="450"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="312"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="175"/>
            <end position="181"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ90810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="736" mass="84169" checksum="647A075EF51CB9B9" modified="2017-01-18" version="1">MSRGGKSKGRNNRGKKTFGQRDDSNNRNWVSTVKENAKWETFYKQQGFIPEEEWETFKKTCQAQLPLTFRITGSRKQSKEVLHLFEKKFLPSLSNLTHEEVQLDAPFALPWYPENLGWQLDASKSVIRKNEQFAKFQRFLVLENSIGNISRQEAVSMIPPMFLDVEPQHRVLDMCAAPGSKTAQLLESLHKTTAEPTGFVIANDADFRRSHMLVHQLKRLNSPNLLVVNHDAQFFPRIAMKEKNDKKPEFIKFDRVLCDVPCSGDGTMRKNINVWKDWNPQGGLGLHTIQLNILMRGLKLLQKNGRLVYSTCSLNPMENEAVIAQALRLFNNDPFNIKHNEKIRLVNCTDKLKGLKRAPGVSNWKVFDRNMDIQESFNPENPKMKKSYFPPTNEENDEFQLQHCVRVYQHMQNTGGFFITLIEKYNTEETQSSTNEEEKATEVVSKEKEQEEEEEEEEEKEVTKKLKTTEGAAVATKKEKLPRDANEEPFNFIDPNHPELTKCWNFYGIDDKFDKKNCLVRNATGEPSRTIYTVSSDLKNIIENNTDRLKLIYSGIKLFVAQRSDIECNWRIQSESLPIVRNHIANNNRIFEITNPEFLKTLMTISFPNFEELSKHDAKIAQEIKEASTGCAFLLFNRQDDNLENLFLPIWNGSRCCNLMISKEDTHELLFRVFGIETTNKDLKVGQDDGSAAKKANEEKKAAAAAAAAAAVATTTTXSAEETTAQEETPAAEPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV81</accession>
    <name>A0A1E5RV81_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Tyrosine--tRNA ligase, cytoplasmic</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g521</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV81"/>
    <dbReference type="InParanoid" id="A0A1E5RV81"/>
    <dbReference type="OrthoDB" id="19141at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051270">
        <property type="entry name" value="Tyrosine-tRNA_ligase_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586">
        <property type="entry name" value="TRNA-AMINOACYLATION COFACTOR ARC1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586:SF43">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="5">
        <location>
            <begin position="55"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ90860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="240" mass="26713" checksum="1B6276C56E95F214" modified="2017-01-18" version="1">MPTLMTGFVRRFPIQQLCKAQTAQRVHARPTCIXQLNSYSTTTPPTSQPVKQNSPFELLSLKIGKITAIQHHENADSMYVEQIDTGKFTADNMSEPIQVCSGLKNYIPINVLQDSLVVVADNLKPSKLRGIKSYGMLLCGSDKNNDIVQTCKPDIRSNKSIDTNLLIGQQVKIVDEKTLSPKKIKSKDLENILSRLYVGDNNHVVLNVDGTEHALYITPKESDVKIPIVVDDLPRGTKVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVI1</accession>
    <name>A0A1E5RVI1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Endoplasmic reticulum vesicle protein 25</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g560</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVI1"/>
    <dbReference type="FunCoup" id="A0A1E5RVI1">
        <property type="interactions" value="1143"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVI1"/>
    <dbReference type="InParanoid" id="A0A1E5RVI1"/>
    <dbReference type="OrthoDB" id="759142at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184931" evidence="9">
        <location>
            <begin position="31"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="10">
        <location>
            <begin position="43"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ90960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="221" mass="25378" checksum="D94AAB65EADC2110" modified="2017-01-18" version="1">MFGLLKQSIATFLICLCVLISISHKSLTQALHFELESKPVPEPYCIRDFVGEGQLVVVEVNSDGNVGDGQVLNMYIRDTTGNEYRRKKDFAGELRIAFTAPSVAAFDVCFENTLQIRGKSLYRNVELDIEVGSQARDWNKIQASEKLKPVEVELRRIEELTDEIVDELHYLKAREERLRNTNESTNKRVKLFSIAIIFLLLGLGAWQVNYLRHYFKSKHII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVU6</accession>
    <name>A0A1E5RVU6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">U3 small nucleolar RNA-associated protein 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g328</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the WD repeat WDR3/UTP12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVU6">
        <property type="interactions" value="1224"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVU6"/>
    <dbReference type="InParanoid" id="A0A1E5RVU6"/>
    <dbReference type="OrthoDB" id="407922at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001139">
        <property type="entry name" value="DIP2p Nucleolar protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000157">
        <property type="entry name" value="WD repeat domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000178">
        <property type="entry name" value="WD repeat domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007148">
        <property type="entry name" value="SSU_processome_Utp12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051570">
        <property type="entry name" value="TBC1_cilium_biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19853">
        <property type="entry name" value="WD REPEAT CONTAINING PROTEIN 3 WDR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19853:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25172">
        <property type="entry name" value="Beta-prop_WDR3_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04003">
        <property type="entry name" value="Utp12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="71"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="113"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="155"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="386"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="468"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="559"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="601"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="643"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="domain" description="Small-subunit processome Utp12" evidence="8">
        <location>
            <begin position="797"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="701"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ90853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="939" mass="106430" checksum="4663756BE9D93F02" modified="2017-01-18" version="1">MVASYQRFEQDSAFGVISSAPSSVWIPSANNTVGQLITAGLENINVWDIKSGELDKKFKPDTAPVGSVDARSNKPSQITSIAYHPNSQLVATGYSDGMIYVWDWFSESIIMKFSGHNSMVTVLQFDATGTRLYSGAADSNIIVWDLVGEVGLFKLRSHKDMITGIWVDSDSEGNDQWLVSSSKDGLVKVWDLKSKQCVETHMAHTSECWGLAIDQELGLCCTLSNDSNFKIWELDLQKPDGTKLVEKGTMEKQSKQRGICIKFGTNPQGVQFMFIMSNDKTVEIFRIRPKDEISKGLKRREKRLREKGMEDEEIEETLXKSYSSIIMHSFHMIRSASKLKSCNWTTLTGKTLNLVVTTATNSIEYYDINYEKRDALGSSKVYTVDLQGHRTDMRSIDMTPDGKLLSTASNGLLKIWNLKTEKCLRTFECGYALCSKILPGGSLVVVGTRAGELQLFDLITSQMLECVETAHEGAIWSLDITYDGKKLITGSADKTVKFWNFSIDEAEKQMKLYHDTTLELGDDILCVKLSAQDXFLAVSLMDNTVKVFYFDTMKFFLSLYGHKLPVLSIDISDDNKMLITSSADKNIKIWGLDFGDCHKSIFAHQDSIMNVIFIPGSHNFFSCSKDADIKYWDGDKFEMIQKLSAHQSEVWGLCINADGDKLVSCSHDHSIRIWSETDDQVFLEEEREKEMDEMYEDTLLNSLETTENDDPSKTVKQNGGEDEEADQQQVSEVHKQTLESLKAGERLMESLDMGIKELELREDYNKKHGEWAQAGKTGGEPMKPATNAILLALQKTPEDYILDTLLKIKASQLEDALLILPFSYVLKFLKFIDYLIKDKQKLMQYLSVIVKNLVFVIKSNYKELVSQKNVDLKLQIQRVKDKLRKALASNVDDLGYNLQGLKIIKSEWDLKHNLEFVDEYEQSVQERKTSKKRVFEKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZ77</accession>
    <name>A0A1E5RZ77_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Oxidoreductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g244</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000002">
        <property type="protein sequence ID" value="OEJ92136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZ77"/>
    <dbReference type="FunCoup" id="A0A1E5RZ77">
        <property type="interactions" value="278"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZ77"/>
    <dbReference type="InParanoid" id="A0A1E5RZ77"/>
    <dbReference type="OrthoDB" id="64915at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006740">
        <property type="term" value="P:NADPH regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42840:SF5">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42840">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="4"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase C-terminal" evidence="2">
        <location>
            <begin position="144"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ92136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="350" mass="38247" checksum="6107ADE266529F39" modified="2017-01-18" version="1">MAVLNVGIIGTGIFARERHVPSYEALGDKFKVKACFNRHKEKALAFAEFAHIDPSKVYDNAEELIKDPEIDLIDVLVPNQFNLEFVQLAAKHNKPILLEKPVAATVAQTKEIVNIAKHCDQPIGISENWLYLEAGKVLKKHLKQIGPIVSFTYNSTGPFVSKNKYATAWRAKPEHIGGFLSDGGVHQLALLTDVLGPVASVSALTKQVRQESGTDDIVYSTVKMEDGDNVFGTFTYGSAFGATDKWTVFKIYGENGSVFLNLSNKGAPVLTLNVGPNAETFEIKNENITFTENESFGVTDEFSNMYEAIVAKDKTLVKGTPAVCGHHLLIVAAFLESSKQNGSNVAVEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZC4</accession>
    <name>A0A1E5RZC4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="6">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g88</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZC4"/>
    <dbReference type="FunCoup" id="A0A1E5RZC4">
        <property type="interactions" value="1103"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZC4"/>
    <dbReference type="InParanoid" id="A0A1E5RZC4"/>
    <dbReference type="OrthoDB" id="333239at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061136">
        <property type="term" value="P:regulation of proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044635">
        <property type="entry name" value="UBP14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43982">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43982:SF1">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="6"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="114"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="363"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="363"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="381"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ92307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="514" mass="58485" checksum="BC96B0A459E3DB72" modified="2017-01-18" version="1">MSEVSLEFVIKHAGKTYPIKLSENAKGSDLKNEVVSLTNVPIERQKYMVKGGKLNDDVLVSTLIKPKQTVMLLGTPDAGLIHAPAKKQKFLEDKYQPEDLHNTSKEHANPNIPLGLKNLGNTCYANSTLQAIYQIDPIREAVLALDPSTNASNPEDEPHLKLIKELKLVFREMRDKKQPQVFPGTFIMQLRQVYPNFKEIDQATSIYKQQDAEEMFTLLMNSLRIVFPKVMDCSTIEFLTKITDLNNPSDVSYKNDDVDTKLQCHISSSTNFLKNGLQNSLTETLEKNSSITNTNSTFEYSKKITRLPEYLTVQYVRFFWKKSSGKKSKILRKVTFPFQLDVADLLEPEYAAKKIDTREKLRDVDRRKQEEERELRKQKQTPNSSNTSLDVPSGMTQEEFDAMDPMELEVLKKSLEESRKEKWAAEFKSEFPENLSGGENASCLYNLVAIITHKGANSESGHYQSFIKDTDNDEVWYKYNDDQVSTLSKEKVELLAGGGESDSALILIYKAFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZK1</accession>
    <name>A0A1E5RZK1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Vacuolar basic amino acid transporter 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g159</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RZK1"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17502">
        <property type="entry name" value="MFS_Azr1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000373">
        <property type="entry name" value="Vacuolar basic amino acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF198">
        <property type="entry name" value="AZOLE RESISTANCE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="308"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="349"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="450"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="476"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="506"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="547"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="615"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="96"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="636"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="638"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ92402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="655" mass="72237" checksum="0FB6FFE86A5FC182" modified="2017-01-18" version="1">MGWLDIHKSNSTRVHHDETHSGSNXFLYDTHKTYSAELXNLASTDTACFADESEEKKGQLKEETEELLQYSMSNDESGLNGTEEPQYYEGWKLVVVQASLNLILFIAAXDIVIVTSAIEKISEQFHDYSKSAWIITGYSLPSAVCCLIWSRIAQKLGKKLSVALAVVVFEXGSLIVAVANSMNMLIGGRVIAGIAGSGIQTLMFLVGATVVPESKRPIVFSILSFSFTVSSVLGPFIGGAFTNAPSGGILSWRWCFYINLPIGFFALFIFCIIYKDPDCDVIEIYRYWKINFXPNLKKLKTKKFYTSLMLETLVSFDMIGFLSSSGGYILTLLAFTFASGENWTWKSGIVLXFFIIGIILIIFSFIFEYTYYDKMVALLXKKYTHNKAIDFQFDLAAPLFPQVAISNTYIGCANAAALFVILAYGCQANYVIQYFQLVFNYSALKASINFIPFMISISIVCMFCGVTMARTGHLKPFIIIGGITAIVGNALLSIMDGASNEAEKIIFLVIASSAFGFVSQSSLISCQMQLDKEDPKYMTNFVGVTAVSNFCQTLGISIGSVISTMIFNLSFIDKAKKLDPPVTLTADDIVSYITSNFESPTSKISKMVSSSIQNVFYFSTAIAALSFILSLFTSSKKSPTDKKSKKLAXDEKEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZS3</accession>
    <name>A0A1E5RZS3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Holocytochrome c-type synthase</fullName>
            <ecNumber evidence="10">4.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g211</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Lyase that catalyzes the covalent linking of the heme group to the cytochrome C apoprotein to produce the mature functional cytochrome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>holo-[cytochrome c] = apo-[cytochrome c] + heme b</text>
            <dbReference type="Rhea" id="RHEA:22648"/>
            <dbReference type="Rhea" id="RHEA-COMP:10725"/>
            <dbReference type="Rhea" id="RHEA-COMP:10726"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="ChEBI" id="CHEBI:83739"/>
            <dbReference type="EC" id="4.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the cytochrome c-type heme lyase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.17" evidence="10"/>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZS3">
        <property type="interactions" value="343"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZS3"/>
    <dbReference type="InParanoid" id="A0A1E5RZS3"/>
    <dbReference type="OrthoDB" id="1158011at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004408">
        <property type="term" value="F:holocytochrome-c synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000511">
        <property type="entry name" value="Holocyt_c/c1_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12743">
        <property type="entry name" value="CYTOCHROME C1 HEME LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12743:SF3">
        <property type="entry name" value="HOLOCYTOCHROME-C SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01265">
        <property type="entry name" value="Cyto_heme_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00821">
        <property type="entry name" value="CYTO_HEME_LYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00822">
        <property type="entry name" value="CYTO_HEME_LYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0349">Heme</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="9 10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="8"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ92336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="328" mass="37098" checksum="7844D1EAE23909C0" modified="2017-01-18" version="1">MGLFWADSTGNEQSSANKAQTANSSLFWSSSAPLNNTQKQTSCPHKPAVTAGGKTDQVSDRSNSQAASACPVMHKNSSTTEPPTDISACPVMHKTPSSGGTNDTTHINPLNNMPSHLPDQKQDWQKLDLPTERTLSSIPKGDGSPWEYPSPQQMYNAMIRKGKXDPNTGEEIPEDAVESMVFVHNFLNEGCWQEVLDWEEKYCSKEVPTKLLRFMGRPDDLSPRARLFHYLSYVFPSKFNRTLPFDRHDWYVLRPDPTSQLNGNHPGYKEVRYVIDFYEGPDDQDGMPTFNLDVRPALDNFENGKDRVIRWAKPIMEQVFESDKKNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZV3</accession>
    <name>A0A1E5RZV3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein SYM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g112</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZV3"/>
    <dbReference type="FunCoup" id="A0A1E5RZV3">
        <property type="interactions" value="572"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZV3"/>
    <dbReference type="InParanoid" id="A0A1E5RZV3"/>
    <dbReference type="OrthoDB" id="430207at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF17">
        <property type="entry name" value="PROTEIN MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ92497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="204" mass="23225" checksum="67EDFCA15919D635" modified="2017-01-18" version="1">MSSFIKFYTQSLQKNPKTTNAVSTGFLFGLGDIIAQLAFSSPPLSKQHNDVAKTMGKNLANLETHNHKPYDPYRTLRAVAYGSVIFSPIGDKWYKFLQNKVYSPKLSNMTNTLARVAVDQLCFAPLAIPFYFGCITLMEGKTWQEAKLKINENYKTTLLANWSVWPLFQAFNFAVVPVHHRLLAVNTIAIFWNTFLSYRNSIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S0D8</accession>
    <name>A0A1E5S0D8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g100</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Mitochondrion inner membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the small Tim family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5S0D8"/>
    <dbReference type="FunCoup" id="A0A1E5S0D8">
        <property type="interactions" value="912"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S0D8"/>
    <dbReference type="InParanoid" id="A0A1E5S0D8"/>
    <dbReference type="OrthoDB" id="1551503at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.810:FF:000008">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit TIM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050673">
        <property type="entry name" value="Mito_inner_translocase_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13172">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 13" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="14">
        <location>
            <begin position="14"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ92475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="87" mass="10233" checksum="1360729A4BA13C52" modified="2017-01-18" version="1">MDTLNSKEQQEFQKVVEQKQMKDFMRLYSNLVERCFSDCVNDFTSSKLSNKELSCVQKCSEKFLKHSERVGQRFQEQNAAMAQNLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S0J5</accession>
    <name>A0A1E5S0J5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g72</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5S0J5"/>
    <dbReference type="InParanoid" id="A0A1E5S0J5"/>
    <dbReference type="OrthoDB" id="368909at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sm" evidence="1">
        <location>
            <begin position="5"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="104" mass="11571" checksum="9204383769B82E94" modified="2017-01-18" version="1">MVEIQDLKSFIGSLLFIDLRPTQVYLERHLCGTLVASDNNLNLLIENTLETTKQKISSKATANNISELKRELGLVSVPFGEIKSIKIATSDIQKMEHAQANCKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0B9</accession>
    <name>A0A1E5R0B9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g4558</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0B9"/>
    <dbReference type="InParanoid" id="A0A1E5R0B9"/>
    <dbReference type="OrthoDB" id="2430343at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ80342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="119" mass="13707" checksum="E0A9DDF1EC3683B3" modified="2017-01-18" version="1">MSPKTLPDLRFASTIRNSFQNQYSILMKNKLAKEGKLGSAEIKPFALEQDNYLRARVVATVVLKQVIFMPFLQSIVYAELLILSRPWLIMIVRNGVRAGMNVKSWMDKFILFRKPQAAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0C7</accession>
    <name>A0A1E5R0C7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent RNA helicase DBP10</fullName>
            <ecNumber evidence="4">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ATP-dependent RNA helicase dbp10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">AWRI3579_g4480</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits and is required for the normal formation of 25S and 5.8S rRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DEAD box helicase family. DDX54/DBP10 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0C7"/>
    <dbReference type="FunCoup" id="A0A1E5R0C7">
        <property type="interactions" value="1119"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0C7"/>
    <dbReference type="InParanoid" id="A0A1E5R0C7"/>
    <dbReference type="OrthoDB" id="10261375at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17959">
        <property type="entry name" value="DEADc_DDX54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000865">
        <property type="entry name" value="ATP-dependent RNA helicase DDX54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012541">
        <property type="entry name" value="DBP10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033517">
        <property type="entry name" value="DDX54/DBP10_DEAD-box_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF8">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08147">
        <property type="entry name" value="DBP10CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01123">
        <property type="entry name" value="DBP10CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 21" id="KW-0347">Helicase</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="20">
        <location>
            <begin position="134"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="165"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="418"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="394"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="944"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="16">
        <location>
            <begin position="134"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="394"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="944"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="992"/>
            <end position="1002"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OEJ80346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1010" mass="114240" checksum="3CB09BC8403F730A" modified="2017-01-18" version="1">MPVNLKRRASDASNFDSDGSASDNENYDIAGDIALNSDSSSEEDNDNLEGEGLQDVIDFSDDESQKKQQQKPKTAQSKNKSDFAFPLLEDNSQQKANAGKENDDDDDINDYFNLQLEGNAQNNLPGQGGKVKKGSFASFGLSKSLLRNISKKGFKQPTPIQRKTIPLILQKRDIVGMARTGSGKTAAFVLPMIERLKMHSAKIGARGVILSPSRELALQTYKVFKEFSKGSDLRGVLLTGGDSLEDQFSMMMSNPDVIIATPGRFLHLKVEMNLDLKSVEYMCFDEADRLFEMGFQEQLNELLYALPLQRQTLLFSATLPNSLVEFAKAGLTNPVLVRLDAETKISENLEMLFVSTKKEERESNLLYILQDVIQLPVASQDEIIRFTKLQKANDEEDEEDYNDDSNDAEKRAKRRKEKNKKFFKRLPSANELPSEKATIIFVPTRHHVEYISNLLKDCGFLVSYIYGSLDQHARRQQLYNFRLGLTSLLVVTDVAARGIDIPILANVVNYSLPASSKIFIHRVGRTARAGNKGWAYSIVSDSELPYLLDLELFLGRKILLTPMYEAGCAMMKHKWIAEGNAVYEFKAPKISYVNRLVLGSAPRLELEDMSDLYKNIMDQNFELQTLKGVAMKAEKLYLRTRQQSSAESIKRTKEIVNAGWDEQNLRFGKNVEKEKLDFLSKLQNRNNKETVFEFARNQDDDMSVLMKRRRKKLAPMQARTKERRELIEKERLAGLRHNLEDEVLEHSQKVDENGDEFVDVASGYTVPESILSQFEDGDKVLDLQEEAQKASKQKQKPKSFKDPQFFLSHYAPASDIQDKQLAISSGFTNDAASAAFDLVNDDKVQVHKQTAKVVWDKKRKKYVNAHGLDNKKYIVGESGQKIPASFRSGKFEEWTKSRKLGPLKVGARESSIPNNLLSDPGKAFVPGGKKNGKFMHKQVKAPKMPDKYRDDYHTQKKKVSNALESGVSVKGYNAPGTKSELKSTAQIRKGRVMKDKRRDKNGRPSKKRKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0S5</accession>
    <name>A0A1E5R0S5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Valine--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="4">6.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Valyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AWRI3579_g4613</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>tRNA(Val) + L-valine + ATP = L-valyl-tRNA(Val) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0S5">
        <property type="interactions" value="1237"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0S5"/>
    <dbReference type="InParanoid" id="A0A1E5R0S5"/>
    <dbReference type="OrthoDB" id="629407at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00817">
        <property type="entry name" value="ValRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.380:FF:000001">
        <property type="entry name" value="Valine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000009">
        <property type="entry name" value="Valine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000020">
        <property type="entry name" value="Valine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000078">
        <property type="entry name" value="Valine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.740.10:FF:000005">
        <property type="entry name" value="Valine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.380">
        <property type="entry name" value="Valyl-tRNA synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02004">
        <property type="entry name" value="Val_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037118">
        <property type="entry name" value="Val-tRNA_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019499">
        <property type="entry name" value="Val-tRNA_synth_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004349">
        <property type="entry name" value="PRK05729.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00422">
        <property type="entry name" value="valS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946:SF109">
        <property type="entry name" value="VALINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="VALYL-TRNA SYNTHETASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10458">
        <property type="entry name" value="Val_tRNA-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00986">
        <property type="entry name" value="TRNASYNTHVAL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 14 20" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 14" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="17">
        <location>
            <begin position="178"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="18">
        <location>
            <begin position="851"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="domain" description="Valyl-tRNA synthetase tRNA-binding arm" evidence="19">
        <location>
            <begin position="1059"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="112"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="1099"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="116"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF10458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OEJ80490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1128" mass="128549" checksum="65A87C28515E60DE" modified="2017-01-18" version="1">MLKNQSLFAVKNFAVSSFGLYRFQHSRLLSTSPIYLQQHSNKKNSTKRHPALVDYKKLNIKMANPDQKIPEVDPTTGEIIINPLKEDGSAKTPKEIEKEKKKAEKILKFLQKQAKKQAPGNNNNGGANGENKKKKEKKVAEPVPEFVDKTVAGEKKILVSLDDPALKAYNPANVESSWYDWWVQNKFFEPEFTESGDIKPEGCFTIPAPPPNVTGALHIGHALTIAIQDSLIRYNRMKGKTVLFLPGFDHAGIATQSVVEKKIWAAEQKTRHDFEREEFVGKVWEWKEEYHSKIKAQIQKLGASYDWSREAFTLSPELTDSVEEAFIRLHDAGTIYRSSRLVNWSSKLNTAISNLEVENKEIKGRTELSVPGYDEKVEFGCLTSYAYEIVDSDEKIIVATTRPETLFGDTAIAVHPDDKRYTHLHGKFAQHPFLDRKIPIIVDAETVEMDFGTGAVKITPAHDANDYNTGKRHNLEFINILTDDGLLNDECGPEWAGVRRFDARKLVIAKLKEKGLFIGQTDNEMVIPTCSRSGDIIEPLLKPQWWVAQETMAKEAIKVVKNGEITITPKSSEAEYFHWLENIQDWCISRQLWWGHRCPVYFINIEGTSDEENVLRRTKDKHWVAGKTEEEARGKAKLRFPDDKYTLEQDEDVLDTWFSSGLWPFSTLGWPKVTEDMDRFYPFSMLETGWDILFFWVVRMIMLGVKLTGNVPFKEVFCHSLVRDAQGRKMSKSLGNVVDPLDVIXGIDLNDLHAKLYNGNLDPREIEKAKAGQKESYPNGIPQCGTDALRFALCAYTTGGRDINLDILRVEGYRKFCNKIYQATKFALMRLGDDYQPPSEEKLSGNESLVEKWILHKLTETNKVVNEALDSRDFLNSTSAIYEFWYLVCDVYIENSKYLIDQGTAIEQKSAKDTLYILLDNALRLIHPFMPFVSEEMWQRLPKRSTETSVTIVKAKYPVYNKSYDNIKAYNDYNLVLDTIKASRSVFAKYNILKNGKLYIESASFHEIFEAQKTSIVSLIKAIDEVAVFDKSNKIPEGCVLESVNADVNVHILVKGQVDIDSEITKTEKKLEKIVKSKQSIEKTISSKDFTEKASEEVKQQTQTRLQDVVSEIDGLEATIANLQRLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1A8</accession>
    <name>A0A1E5R1A8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein OCA4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1A8">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1A8"/>
    <dbReference type="InParanoid" id="A0A1E5R1A8"/>
    <dbReference type="OrthoDB" id="6375174at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004861">
        <property type="entry name" value="Siw14-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF70">
        <property type="entry name" value="PROTEIN OCA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03162">
        <property type="entry name" value="Y_phosphatase2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="587" mass="66636" checksum="C7A5514B0ECA8CE8" modified="2017-01-18" version="1">MLVPPVSFGLVEEGIYRCSKVETLNLSFLETLNLRIIIFLSGEEPSRYFKEFFKRSSIEWIAINPNSYTTLTSSGIRNTNNQLELEPTGGGAEKQTIQNSDDTDATXNNKTLTSSIANDYSSGAESNGLLLLNAVVLQKIFSVLLDQSNFNCLLVDKSAIVVGVLRKILKWNISSIVNEYRLFAGKNRNYFSESFLELVKVNVKQKVEQIQDHDTILHSGKHSTSQSLRESMEFHGGTVGAQKDLIESKTTPSFALKPRTRSSSIIVEDEDVVIREQKKGQAGHHIEFHQHPSFGRTSNFDQKKWKNNSHQKVLTEHDLDIFEPEVPGLLLAMIEDVEQIQKQEELERKEKGKKNDIDLLNIKDDSQSSSLSSNALQNNQIKKSASALGIFGNVYRLAFDKPQKADYEYYKSNVDKKLEERNEKIKHATLVSNSVLKIDKHSKIKAPATKALFETASKENRTNKTIKGIIPLTIKPNVYKDEQQSQRNIKLLNKYGANINENPFMVSSWGEADXEQNHDPVELKNTDESDKNIDSEITKTSKNLDPPKNIVNLIIPEEENLPEWFKLQRDIWEKNNTVDFHTESVFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1F3</accession>
    <name>A0A1E5R1F3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidyl-prolyl cis-trans isomerase</fullName>
            <ecNumber evidence="5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3975</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1F3"/>
    <dbReference type="FunCoup" id="A0A1E5R1F3">
        <property type="interactions" value="976"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1F3"/>
    <dbReference type="InParanoid" id="A0A1E5R1F3"/>
    <dbReference type="OrthoDB" id="2530521at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000026">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051370">
        <property type="entry name" value="PPIase_Pin1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000297">
        <property type="entry name" value="PPIase_PpiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10657">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10657:SF4">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00639">
        <property type="entry name" value="Rotamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50198">
        <property type="entry name" value="PPIC_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 5" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="WW" evidence="7">
        <location>
            <begin position="11"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="PpiC" evidence="8">
        <location>
            <begin position="59"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="82"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="170" mass="19408" checksum="EF494421903BA334" modified="2017-01-18" version="1">MSDNKSSVTETGLPKPWTVRYSRSHKREYFFDPETKQSVWEAPENTDQAKLGAYLNNHPLRVRCLHLLIKHSGSRRPASHRSENITLSKEDAIEELQKLRDEIVNEGASFKQLAEERSDCSSYKRGGDLGFFGRKEMQPAFEDVSFKLKVGEISEIVETDSGVHIIKRVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1H5</accession>
    <name>A0A1E5R1H5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1H5"/>
    <dbReference type="InParanoid" id="A0A1E5R1H5"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="228" mass="26275" checksum="BA5FD87E67457CDB" modified="2017-01-18" version="1">MLTSNSSSLTTIKNTVLSSSNAIINLVIREPESINSLNTTNDTPTHSNKDVSNKMTFFYPPLPELPLNKHNLKEYLSNQDNLGSIFFCFWVGLIAVLVLTMMIWSVHDYFTSEVNDIMKTVPLKHEQKKYLTLTQVFLLPEPGKTAYLNMCQQDFEKLLIKERNQRLHVCTSNSGSFEQKARLKKRQAGEFFKNLTRALMKPLHTCTLFKHETKFYPSPNTWPPDLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1T8</accession>
    <name>A0A1E5R1T8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcriptional activator HAP2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g4365</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NFYA/HAP2 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1T8"/>
    <dbReference type="STRING" id="56408.A0A1E5R1T8"/>
    <dbReference type="InParanoid" id="A0A1E5R1T8"/>
    <dbReference type="OrthoDB" id="1097733at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018362">
        <property type="entry name" value="CCAAT-binding_factor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001289">
        <property type="entry name" value="NFYA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12632">
        <property type="entry name" value="TRANSCRIPTION FACTOR NF-Y ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02045">
        <property type="entry name" value="CBFB_NFYA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00616">
        <property type="entry name" value="CCAATSUBUNTB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00521">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00686">
        <property type="entry name" value="NFYA_HAP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51152">
        <property type="entry name" value="NFYA_HAP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="109"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="11"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="115"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="151" mass="17407" checksum="7321FE1A01295AAF" modified="2017-01-18" version="1">MSDTEHAVINETGQTPNLQETTVPVTDDSSNKIPSEVKNVMLSLVGSVATQNQTQEQKLQFLSEATTKELEEQPFYVNAKQYNRIMKRRQARAKLEEILQRNFLERQKRPYVHESRHKHAMRRPRGQGGRFLTAAEIKKLSEEETPNNSNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1Z4</accession>
    <name>A0A1E5R1Z4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Superoxide dismutase [Cu-Zn]</fullName>
            <ecNumber evidence="8">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g4198</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="8">Binds 1 copper ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the Cu-Zn superoxide dismutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="8"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1Z4"/>
    <dbReference type="FunCoup" id="A0A1E5R1Z4">
        <property type="interactions" value="780"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1Z4"/>
    <dbReference type="InParanoid" id="A0A1E5R1Z4"/>
    <dbReference type="OrthoDB" id="2015551at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00305">
        <property type="entry name" value="Cu-Zn_Superoxide_Dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.200:FF:000001">
        <property type="entry name" value="Superoxide dismutase [Cu-Zn]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024134">
        <property type="entry name" value="SOD_Cu/Zn_/chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018152">
        <property type="entry name" value="SOD_Cu/Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10003">
        <property type="entry name" value="SUPEROXIDE DISMUTASE CU-ZN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00080">
        <property type="entry name" value="Sod_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00068">
        <property type="entry name" value="CUZNDISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00087">
        <property type="entry name" value="SOD_CU_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00332">
        <property type="entry name" value="SOD_CU_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6 8" id="KW-0186">Copper</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Superoxide dismutase copper/zinc binding" evidence="9">
        <location>
            <begin position="14"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="156" mass="16307" checksum="A56FF8984E3861B6" modified="2017-01-18" version="1">MVKAVAVLRGDSKVNGVVHFEQTEAKAPTKITWEITGNDPNSKRGFHIHQFGDNTNGCTSAGPHFNPNGVSHGAPTNDIEHRHVGDLGNLETNEQGVTKGTTTDAFIKLIGEDSVVGRTIVIHAGEDDLGKGGVEESLKTGNAGGRNACGVIGLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R224</accession>
    <name>A0A1E5R224_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phosphotransferase</fullName>
            <ecNumber evidence="12">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g4387</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:16126"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22740"/>
            <dbReference type="ChEBI" id="CHEBI:4194"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:229467"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:22741"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the hexokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="12"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R224"/>
    <dbReference type="FunCoup" id="A0A1E5R224">
        <property type="interactions" value="923"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R224"/>
    <dbReference type="InParanoid" id="A0A1E5R224"/>
    <dbReference type="OrthoDB" id="419537at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00180"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008865">
        <property type="term" value="F:fructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019158">
        <property type="term" value="F:mannokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046323">
        <property type="term" value="P:D-glucose import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990539">
        <property type="term" value="P:fructose import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001678">
        <property type="term" value="P:intracellular glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1250:FF:000001">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000092">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.367.20:FF:000004">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.367.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001312">
        <property type="entry name" value="Hexokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019807">
        <property type="entry name" value="Hexokinase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022673">
        <property type="entry name" value="Hexokinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022672">
        <property type="entry name" value="Hexokinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443">
        <property type="entry name" value="HEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443:SF16">
        <property type="entry name" value="HEXOKINASE TYPE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00349">
        <property type="entry name" value="Hexokinase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03727">
        <property type="entry name" value="Hexokinase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00378">
        <property type="entry name" value="HEXOKINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51748">
        <property type="entry name" value="HEXOKINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 12" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 12 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hexokinase N-terminal" evidence="13">
        <location>
            <begin position="28"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase C-terminal" evidence="14">
        <location>
            <begin position="229"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ80944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="488" mass="53833" checksum="5CCCE8AF400909B7" modified="2017-01-18" version="1">MVRLGPKKPPTRKGSMVDVPADLMQQIHGLEAIFTVSGDKLKAIVKHFVTELNKGLTKKGGNIPMIPGWVMEHPTGKESGDYLAIDLGGTNLRVVLVKLGGDRTFDTTQSKYKLPNHIRTTKNKDELWAFIADSLDTFIKEEYPEGVPKGSKLPLGFTFSYPASQDKINEGILQRWTKGFDIPGIEGHDVVPMLQTELAKRNLPIDVVALINDTVGTLVASLYTDGETKMGCIFGTGVNGAYYDVVSDVEKLEERLADDIPKESLMAINCEYGSFDNEHLVLPRTKYDVMVDEQSPRPGQQSFEKMTSGYYLGELMRLALVDLHSQGFIFKNQDITQLQKPYVMDTSFPARIEEDPFENLEDTDDLFTNELKIKTTSVERKLIRKLAEVIGTRAARISVCGIAAVCQKRGYETGHIAADGSVYNLYPGFKEKAHAGLRDIYGWAEESDFSKYPISIVPAEDGSGAGAAIIAALTEKRLAKGLSVGVIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R290</accession>
    <name>A0A1E5R290_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4127</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R290"/>
    <dbReference type="OrthoDB" id="4096620at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="58"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="152" mass="17046" checksum="CA14FB51934C4C01" modified="2017-01-18" version="1">MSYIERSNTPTYXGGSNVNNGSQRPYSVNNPFRNASTDSSINQYKSDLEFQNWVRENQQVQSGSASQGQPFYSRPAGNRSTGSFRSYHSNNSRNSGAFYEDDIIDHYKRSDSPVHMMGPPPSSTGLAVQRKYVKLLFFSCFVAVNGILELXE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R296</accession>
    <name>A0A1E5R296_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Pre-mRNA-splicing factor 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g4231</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R296">
        <property type="interactions" value="469"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R296"/>
    <dbReference type="InParanoid" id="A0A1E5R296"/>
    <dbReference type="OrthoDB" id="440128at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010491">
        <property type="entry name" value="PRP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045075">
        <property type="entry name" value="Syf1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246:SF1">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06424">
        <property type="entry name" value="PRP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PRP1 splicing factor N-terminal" evidence="5">
        <location>
            <begin position="13"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="88"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="6">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="6">
        <location>
            <position position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ81021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="902" mass="104421" checksum="4A8040AED30FB139" modified="2017-01-18" version="1">MYAGRPAFLDQQAPKGYIAGIGRGAVGFDKNQNLLDTKRLPKRLQNEQLLNRKTQLERFQDLEEGERDEQKTFDEIDQRRLLKKGINSGKNSHSDSTSPLESSTNDFADLKKNLSLVSDQEWLNLPEATDLTRRNKRLKVQDTENRKVGYVVDVEAVVAGAGAATTTTLARINEERELLLGKQLDHALSSSGRGIEETKTEKSSAXETLRLLEAADXRTESEIEKTRKMLQLYIKSDSXNPSGWIALTRLEESCKKFKTAKKIIQQACXHIPYNVDLWMENIRLYETTDIPKCQQLTKQALKYNFKEQKIWVKAISLESXRNTKIKLIRTGLMQNPAQEELWKLSIEFEENKQEALKMVEKALEYLPKSXYLWIALYKLRCETNDVXYSEKKQLLGRILTNVPNSKTVWVLACQLEEYEMKLQKKPESNIRMALNSLLEKNDYKGDDVEEWITEAETCSAQDXYPLTTASIIRLYMILQNFTKPDPSLLAKFQKFETKSLIFEHFLVQEKSLSLWSQYYRFGKNSGYLASVFKFWRKHIENLDNAFDFPNCVLLYCKYNSSNGNFEKATSIIEKLLAVKDNRLTDPLYEKLMLANIKILLYQNLRDSADVLFSKLVCNEXKRSLSSRVAYKYIAYLLYKKSDYELVLXRVDKFIAEYPQEIKLYLEKLQILKEEPKFSSKFEQTIXVAKKLFPTQVEFWLYECAKAETYMSSKIVLDQAIIYFSKLPSSKINGAPMEKLYIQKAQIEIDYKSNLQEAKIIIQQALLKYPKSELLYSYFIRIAARLLKPQELKNLFARALKQTENSALILLQIGLQFIXIPEKALKWIDKALIKDQKLGDAWCWKYKLSPTSAAHDILDKVQDAEPNHGDLWVAYSKKNTALRYEMPKNLVRTVAAELVIGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2D2</accession>
    <name>A0A1E5R2D2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Hexose transporter 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g4133</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2D2"/>
    <dbReference type="FunCoup" id="A0A1E5R2D2">
        <property type="interactions" value="1430"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2D2"/>
    <dbReference type="InParanoid" id="A0A1E5R2D2"/>
    <dbReference type="OrthoDB" id="5141738at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000044">
        <property type="entry name" value="Hexose transporter Hxt3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF75">
        <property type="entry name" value="GALACTOSE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="344"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="365"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="391"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="434"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="468"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="496"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="71"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ81067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="571" mass="62706" checksum="C1373597308C57E4" modified="2017-01-18" version="1">MSAATPAAEQQPHVAPITEGEPLSSTGSSVSASELKQDDSAPMMVADENGQQVLPEMPVKPNSAYIGLGVMCLMVAFGGFVFGYDTGSIGGFVAMSGFKEKYGSHNSSGYYLSNVRTGLIVSIFNIGCAIGGITLGKLGDMYGRKIGLMCVTIVYIVGIVIQIAAVHAWYQYFIGRIISGLGVGGIAVLSPMMISETAPKHLRGLLVSCYQLMITLGIFLGYCINYGTKGYTNTAQWRITLGLCFLWALFMLGGMTFVPETARYYCEKDRVEDARESLSKVNKVPKDDPALQAELDTIVAGVEAERLAGEASWKELFSTKTKIFQRLVMGVMLQSLQQLTGDNYFFYYGTTVFAAVGLSDSFQTSIVIGIVNFASTFFGLFFVNRFGRRTLLLWGAAAQVACFVVFASVGVTRLWPDGAGHPENSSKGAGNCMIVFTCFYIFCFAVSWAPLIYVVVSESYPLRVRSKCMAIATGANWIWGFLISFFTPFITNAIHFAYGYVFMGCLVFSFFYVFFVVPETKSLSLEEVEEMWQEGVLPWKSSSWVPASRRGVDYSAEALQHDEKKGFRRFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2F9</accession>
    <name>A0A1E5R2F9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SMP-30/Gluconolactonase/LRE-like region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4228</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMP-30/CGR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2F9">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2F9"/>
    <dbReference type="InParanoid" id="A0A1E5R2F9"/>
    <dbReference type="OrthoDB" id="423498at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004341">
        <property type="term" value="F:gluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013658">
        <property type="entry name" value="SGL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907">
        <property type="entry name" value="REGUCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907:SF47">
        <property type="entry name" value="REGUCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08450">
        <property type="entry name" value="SGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMP-30/Gluconolactonase/LRE-like region" evidence="2">
        <location>
            <begin position="18"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ81082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="381" mass="42442" checksum="CADF70D7A675CC1D" modified="2017-01-18" version="1">MLXEEKVTPLISLPDARLSEGITYIKEYSRYYWVDIFKAMIYSAPVHNPSTDVLSLHISKDXYALDQETQTGFSHSKYAKYPYPDSLFPERVGVVFPTTNSEHKVLFGGKYGLGVADLXTKTWYYRILYSEIQNFTTGSNIAVDHDWTRMRGNDGNVCPKTSSVYIGVMNDFHVGDCTTEGFVCKINLSKSQDTIECVYEGIRIPNSVNFFQSDIYLTDSLQFQILKISNGVNLKHWQHDGNGSHVTXFIDIKAHNKDFESPEPDGSVIINDKAATTGTPTTTTTNXDNPKLITAVWSTSKVQEYSLTTGKLLEEHLVPHAERISCCCVGPLLSEIIATTASLEVDVRGGEPNKDGSLFKITRTDAPQXTQAPSSKKLLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2H7</accession>
    <name>A0A1E5R2H7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">UDP-N-acetylglucosamine transferase subunit ALG14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Asparagine-linked glycosylation protein 14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ALG14</name>
        <name evidence="12" type="ORF">AWRI3579_g4250</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in protein N-glycosylation. Essential for the second step of the dolichol-linked oligosaccharide pathway. Anchors the catalytic subunit ALG13 to the ER.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 11">Heterodimer with ALG13 to form a functional enzyme.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the ALG14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2H7">
        <property type="interactions" value="303"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2H7"/>
    <dbReference type="InParanoid" id="A0A1E5R2H7"/>
    <dbReference type="OrthoDB" id="17098at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043541">
        <property type="term" value="C:UDP-N-acetylglucosamine transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004577">
        <property type="term" value="F:N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013969">
        <property type="entry name" value="Oligosacch_biosynth_Alg14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12154">
        <property type="entry name" value="GLYCOSYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12154:SF4">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE TRANSFERASE SUBUNIT ALG14 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08660">
        <property type="entry name" value="Alg14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ81084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="255" mass="29451" checksum="41FC6632DE3FF65F" modified="2017-01-18" version="1">MSTLINVFIIIILCFVCYVNVTIIKTLNFTPQDKDFKHKKTLKIDKIDNVFVFLGSGGHTGEMLKLLENYQDLFLNNSTNFYLCFSDNRSLINFQKKYDQSTDTLPSYHTKFHYIQLYKAREVGSGKMQSLLSIIATVINLLQKLTVPLIKTCLANFKNKSEQKSVFLLNGPGTCVLISIYVRFFQFLHQLLKPLTAARXSRNMKIIYIESLARVNKLSLSGALIYYAKLYDEFVVQWQELTNVYPDSKYYGILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2P4</accession>
    <name>A0A1E5R2P4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Proteasome subunit beta type-6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g4202</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2P4"/>
    <dbReference type="FunCoup" id="A0A1E5R2P4">
        <property type="interactions" value="1032"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2P4"/>
    <dbReference type="InParanoid" id="A0A1E5R2P4"/>
    <dbReference type="OrthoDB" id="268479at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03757">
        <property type="entry name" value="proteasome_beta_type_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000027">
        <property type="entry name" value="Proteasome subunit beta type-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF2">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ81154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="242" mass="27044" checksum="B8D9FC78A52B9E06" modified="2017-01-18" version="1">MQPIQHNFNPYSDNGGTILGIAGKDFAVLAGDTRQTVDYSINSRYESKVFDVGDNILMSANGFAADGDALVKKFKNSLKWYHFNHTSSISSISELSEQNGQHKMSLKSAARNIQHLLYGKRFFPYYVHTIIAGLDEEGKGSVFSFDPVGSYEREQCRAGGAGASLMMPFLDNQVNFNNQYEPDSHGQVKKKLEYLSLDEVLQLVRDAFTSVTERHISVGDGLEILIVTKDGVRKEFFPLKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4K7</accession>
    <name>A0A1E5R4K7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cytochrome c oxidase assembly factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3558</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4K7"/>
    <dbReference type="FunCoup" id="A0A1E5R4K7">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4K7"/>
    <dbReference type="InParanoid" id="A0A1E5R4K7"/>
    <dbReference type="OrthoDB" id="2100652at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014807">
        <property type="entry name" value="Coa1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042432">
        <property type="entry name" value="Coa1_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28523">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28523:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08695">
        <property type="entry name" value="Coa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="209" mass="23515" checksum="3C5D0EB03E855C06" modified="2017-01-18" version="1">MLSHFARRTVYQTARSAPARLGTTTTLKALSCLSYVNRISNVRKNSAVATDKTAKNASIPAEHPPMTVNTELPDPFKGKTIRRLQFLSFIVAISVALAVVFNYEKTKSPVVSNTLYQLRRSDRIRDLMGSSIDMYGLMPWVSGELNQVQGKINIKFYVKGSKNKRGIVRLVADRENKQHEFLIHEWSLESEEDGVKIDLLGEDSNAKIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4L9</accession>
    <name>A0A1E5R4L9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein MLP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3570</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4L9">
        <property type="interactions" value="1314"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4L9"/>
    <dbReference type="InParanoid" id="A0A1E5R4L9"/>
    <dbReference type="OrthoDB" id="3973197at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057974">
        <property type="entry name" value="NUA/TPR/MLP1-2-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18898:SF2">
        <property type="entry name" value="NUCLEOPROTEIN TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18898">
        <property type="entry name" value="NUCLEOPROTEIN TPR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25785">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NUA/TPR/MLP1-2-like" evidence="6">
        <location>
            <begin position="386"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1128"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
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            <end position="1494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1611"/>
            <end position="1664"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="126"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="186"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
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            <end position="343"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="372"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="470"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="579"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="799"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1027"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1143"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1175"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1199"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
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            <begin position="1437"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
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        </location>
    </feature>
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        <location>
            <begin position="1653"/>
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        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="7">
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        </location>
    </feature>
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        </location>
    </feature>
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    </feature>
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        </location>
    </feature>
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        <location>
            <position position="1582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ81867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1664" mass="186407" checksum="882E09968F2916BA" modified="2017-01-18" version="1">MNNYEIAQELGVDVESIDSLHDEFLKKQLADKLSKWNENSKAEKVQALSGDSNENVGTPNSNEVLNMMNNKDTHIAELTTQLSNTSSSLHDQIDKCNKLELKFSEFKKNNSILDKDFEIEDLKIKVNKLQKSDELKQAELDQLQKQYSEYRLNKNNESSDSDHKLMLMEAEIRSTKEQNTFLVENLSKANKDLKETLCNLSKVKDEHNFDKQHFIQEMDMKQRIIESLEQNLQTVNERCKALQESLSHDNKSVPIEHYNEVVNEMNQNLKQLNEFKLKNHELQELVDSFVSDNGDSQYLVNHLNESNDISVKSINFKILQKELIKEKNQKLSLQRQLKNFVEELECKLPQIDQYDANLRNLEDQLYESSLLIESTSGKIEFFKSENQSLKQQKXQYEHTIKTLQQQRVDLAKQVQTLIYLGVISGKQCVVSEEDCRFVKNLLNNHERLHTNDDAGIQSVISQQLVAFRNVQQLQENNENLLVALRELGEKLESLESQSNDLSDIEGETNNDREDEKVTVEQAKDAILKLEXYNDSLVLKLESVTKERDILKTLCNTGSPTXKDDFFSKKDGHLNEEASNTQLADKAQFLEKQLHIXQANHXEEVARLNKDLQNYIQEKTELTINWQKEINVRELLESQYKLLSDSKLPQLEKEIDILKETITKFSNANLEKDLIHNKTMNEMLSMVQXIESLRSKNGELNDEIVALQHXIEEYKSTLKNNKDNKLYTAHDIDLLKEQIADLSLKNDENTLKLNNALLAKSEAEKKLETKEKMLLAEISVLKNKIEVTGVSTDESHINEYQTLQKDMETAXKNVALLESEIESLKNENNDLQHNISXKDQEIKLVNESLNRWKINCSNVSENFKELEKTKNKEISDTEERYEDRIAKLEEELNNKSANLKLKNDEIFRELNLEIENLQQSLSTVQADAESKSKALLEREQPQATEDQLENLKKQMDEQLQERIESIKKKSREEIRQLVDKKRAELARSFEKSVETKANTTIAERISKIEEDFKTKIEDAKKKASEDAEKRSAMKIKILENKLKKMKEANNQSSSQXSGSNELARPITALSNTSFAKPSLSSPSAMSPLTPNMTAFSGFGQTNAPXNFPVSNANNFSKMPSVFHSANITSGNTTSNVFGKRPFENVSSTNKETEGSSTLQNSLFXSSTFSGAHFVSSXAPVPSSXSNASXAQENXEKRRKLSDEXHEVKEDMQNTTSNLTESAMDKKPASASEGVPEPGSTAFLSSFPATETNAKVESVVEKSGSPETFKELSPLKXFSTSQEGHTGGATANAAQGPESENSPLQNKISEEETANLNDELMPDADLNEENSITXDKELGSKSPPSTESAIAEKTGXIAESENGTAEELLPLPEYTDDAEKEPSSFAXISSQGLSGSPVDSTLETQPATFNEGLSMAGEVSGARATASHDTSLQPVXLTQGXQAQTADANNAYGLANGSGTEEGKEDGXDTLPAAAQEDQLGEQDFEPEQASSAAANVVLDQENATTSFASHTDETAAHLKXNDETVPLSKPIESLETLDTTRASDLHVESXHPLLSESNQFSLDTFVQQNNNAQTEFIQAQTDLEQENIFVEERASEQQTTAFXFVASEPXRAEATGTPMGEETPINEATVTTPLAGTTPITQAEEVPDALAENEQASTPDSVEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4N1</accession>
    <name>A0A1E5R4N1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Magnesium transporter ALR2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3754</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="OrthoDB" id="3970610at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010961">
        <property type="term" value="P:intracellular magnesium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Alr1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.20">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044089">
        <property type="entry name" value="Alr1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045861">
        <property type="entry name" value="CorA_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21535">
        <property type="entry name" value="MAGNESIUM AND COBALT TRANSPORT PROTEIN/MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21535:SF55">
        <property type="entry name" value="MAGNESIUM TRANSPORTER ALR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143865">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="534"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="740"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="771"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="271"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="44"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="74"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="146"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="158"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="191"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="308"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ81876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="878" mass="99179" checksum="8B07FB6307F8A2B6" modified="2017-01-18" version="1">MEQITRSDSSSSSNTFELXDEHNRIVTSDNEEHDDIMLAPAESVKTEDHNELYDHRDQNYSVLKPQAQQMPENEQGRDESSRLPSFERTRSSFGGSDTHEDVHVKIPTPHGHHRPDAAKMSVPHMKLSNQGSTHDIIPQKPRRKSVASSINSMRLNKTKSNTSVSSTISKTKHTNNNNPGNANRGRRNSMDSDDSNDTQETEEDVCFPLIEFTHDRVNGIDFDELEEYARDEIQFNLMQQQIQQAQNGVFPFSESSSTISKAALKYTPSVKTRATSNNGGTAVASKAPTSASAVIAPPSKNEPQEFEPLQKKPPKEQKQNRNFQSKNAISFGYNKVENEDTELEDSSVTTTTTEARPKEFPNNAGIHNYEERNQTPYLPNQTQQYQQEQQGQQNSLYHSAGSHNIPLADFSNPASTAPVFESMYHAPDRFSFFLSEMEETIHAPSISSLLQKSGAPSFKTLFQNGEPTWWLDCVCPTDEEMKCISKCFGIHPLTAEDIRMQESREKVELFKHYYFVCFHTFENDMESEDFLEPINVYIVVFANGVLTFHFGPEIVHCANVRKRVRQLRDYVNVNSDWLCYALIDDITDSFAPVIRSIEYEADVIEDSVFMARDLNFADMLQRIGESRRKTMTLMRLLAGKADVIKMFAKRCQEESKGIGPALTSQVNIANLTATNPQMNSQPRADIALYLGDIQDHVLTMYQNLLSYEKIFGRSHANYLAQLQVESFNSNNKVTEMLSRVTMLGTLLVPLNIIPGLFGMNVRIPGDNGSNLGWFFGIIGVLIIVSGFALWFSKYWLNKIVDPETLNEASAAGKSTMSTFKGNSIFNKSVKNNSRTNVNDNLNDIQSRLPPLPRHYSFNKKPGYARSVNSLPSKYGRYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4P4</accession>
    <name>A0A1E5R4P4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein MSN1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3768</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4P4">
        <property type="interactions" value="239"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4P4"/>
    <dbReference type="InParanoid" id="A0A1E5R4P4"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022210">
        <property type="entry name" value="TF_GCR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14596">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14596:SF72">
        <property type="entry name" value="ZINC FINGER PROTEIN MSN2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12550">
        <property type="entry name" value="GCR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription activator GCR1-like" evidence="2">
        <location>
            <begin position="829"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="419"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="484"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="694"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="917"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="423"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="489"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="499"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="525"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="694"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="719"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="735"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="930"/>
            <end position="940"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ81862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="983" mass="108548" checksum="2E8B70D7E1E998EB" modified="2017-01-18" version="1">MSFGDRLGDLESKTARYEAFFQNLSSRLDFNFQIYDKLLEAQQQQINTMASTMLDIMNDEAXHRDIIKAKYSDILHGVYATNDLSLLRSKGRGGIATSITVPPPSSALSGGSIPNGTSNGNAYNQGTNNDTKLYQKMSHQNISRNYDLNNDDGLDRETRGSTLKRMKHSGVTTDTTGSSNGNMQNNNGGLFALNMNAHQPSQHRRSLSEVTAAAAAAAAALAVEEAEAADTAFNINLFNQEHFLGSQHMVRTKLPQNKTTNDHKTNNTKEKDGDIGNKLYQESQSSRDTKRDNRSRNQKNTIINSRNKDTAQHLTNDRKNPSLDANISNKVSKSSRHQSVPANNNLLNSLIAEQELSPYLNVIQANGLSGTGLSARFQQHSISHDPNNSTSYFSQYTQQQQQQQQQQQQQQQQQQQQQQQQQISQKFPQRANSTSFIPEDHTDIGRQFDEQFGAQFSDFDSIHKSNNGISNSAPNTTMMTGLHERRRMSSGTSGSIGSNEHKLGHPLDSSESRQHRNDTQLGKGLPLPNNLSMPLSLNHSQHQPQNSQYQNVSLALQEGDDKAISLPQLNNDMNAVSSEINKHMLRNNNLDGSTINRGINRHDMNGGTNDLDGHSVNENNGATSNFSGSPIDFQSQHLLNQATSQNNIAQASGLFKLQRSASTPSLVTENPINNLSSSSVNMISNLLNNTESRGISFKSQGNEPVELSSTTAEKVAKPTTKNLNASLNANITEKNSNNNGNDNDNDXDNGNGNGNDNNGNGNDNDNNGNVNDNDNDNNMNSNTNTTSNALPGSSPSSSRSIRSGLTDMSSSTSHITGLIHHKKNFTGHFEFLASPRTVADIWNEYTQGYNSQLPIRDMESIYGNSWRKSAANQRRFNRRKVLYRAIETGLMRGFPLEKCITMLEDCRKTYITDELMPGSDGSVNPKHGLEQSSTTGNQSSTDKRFSDKYPNYMIEEGTGFKYKLNSVGWLCHPKNLPEALKYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4X5</accession>
    <name>A0A1E5R4X5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3632</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R4X5"/>
    <dbReference type="OrthoDB" id="4036433at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="329" mass="37558" checksum="848614900CF0AD71" modified="2017-01-18" version="1">MQKATGVNPHISTANFESLLLFKLICSELEKTTNYYEIHRLANLTSVSDVTNVKTTDNYLRIXYWIXTDSRTFVATSNKLVASAFLRGLLIFIFGAICREALNIMYLYVTKQIILLNSKIKAFTSHALQKLRKEMQQLKIKXEQKXNETNIQRFESVFIKWSKETXATQKELKNDINTCLCLISNLKVQSXCEENIXKVDAFTNNSPSLINXFAKPLXSKDVEQISXICENCQATXFLKSGEHQTPTLKPFNCSAHTKRNSCQIFQVAEHQRLSNNKPTRYLKPXFFIGSSPSSTQSTSKXFDANGKPYRRIFVPGKGWISRARYFGQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R562</accession>
    <name>A0A1E5R562_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Ubiquitin-related modifier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">URM1</name>
        <name evidence="7" type="ORF">AWRI3579_g3701</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by the MOCS3 homolog UBA4. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as AHP1. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 6">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="5">C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of UBA4, then thiocarboxylated (-COSH) via the rhodanese domain of UBA4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the URM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R562"/>
    <dbReference type="FunCoup" id="A0A1E5R562">
        <property type="interactions" value="892"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R562"/>
    <dbReference type="InParanoid" id="A0A1E5R562"/>
    <dbReference type="OrthoDB" id="10248987at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034227">
        <property type="term" value="P:tRNA thio-modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01764">
        <property type="entry name" value="Ubl_Urm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03048">
        <property type="entry name" value="Urm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015221">
        <property type="entry name" value="Urm1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14986">
        <property type="entry name" value="RURM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09138">
        <property type="entry name" value="Urm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037379">
        <property type="entry name" value="Ubiquitin-related_modifier_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="modified residue" description="1-thioglycine" evidence="5">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)" evidence="5">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ82047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="103" mass="11500" checksum="2DB9990E4B4CF8F0" modified="2017-01-18" version="1">MSVALTVEFLGGLDAIFQNQRKHKVTLDEASGKEALKLRDLITYITNNMIENENDIPVFIQDGTVRPGILTLVNDTDWELCDEEEYVLENGDVISFTSTLHGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R575</accession>
    <name>A0A1E5R575_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HDA1 complex subunit 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3510</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R575">
        <property type="interactions" value="63"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R575"/>
    <dbReference type="InParanoid" id="A0A1E5R575"/>
    <dbReference type="OrthoDB" id="3647690at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070823">
        <property type="term" value="C:HDA1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12360">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038609">
        <property type="entry name" value="HDA1_su2/3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021006">
        <property type="entry name" value="Hda2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026216">
        <property type="entry name" value="HDA3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11496">
        <property type="entry name" value="HDA2-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02093">
        <property type="entry name" value="HDA1SUBUNIT3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="169"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="222"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="560"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="180"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="225"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="238"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="252"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="735" mass="83249" checksum="D2DE1F183E7B4348" modified="2017-01-18" version="1">MDLSKILDAAPQLNIVDDDVKHNKTEDTSGDYWLPTPLSTEQSVIYDQIISLHYSDILKYFSGDSVGIDKQASVQNEAHFPEDVIKHSLDTMLFNLETILLHPYLLLTHNLPRSLTTKDTPNSLCTQSGKCKVLRDLVNLVSKYETNTAIVCRGDVSLNDWSFGQRSVAASKKDGPTSDSNATFESSTNSNNSANNYKMVDMLEALLLGSKVNIKRYDGRKXIKKSNSGXSTNSKESLNSHDSEPAEEKETGSTGASKSNNSGGRPCTVHIFSSSNLDEVHKKLDKSIYFDLMICIDSSVPANVSTKLNKYPGIKKILTMNREFPAPIIRVASINGMDHAALYFQSKWPNFETKNLAVRDNKVLKNVVATSVVLRNQAGILPPALKPVYETNLEFLSDWIASPDLAWPLPKLYAIPEFKRFDVERSLLTKFNNFNPNKAQSPLXDLKCKSINSSKADEGTLPYLQNDISEKMYRAINPSADDAFYQAKRCQNEYRSNPLLRKNMSTLTGISTTDEKYFLDNAFVTHELIYALSSTYRYXQLEKSELDSYTLYDPQIELQTKSLTEMFEELNSKVAHAEKQIKNDNVTVSRFLRVQDNTRSQIEKFGKQNIPKLILLKKELEKMKKSFELKCTEKEYMSAEIAKASKTIDENNHQITAMKAEIKNTNLHINSKIELDKETSIKLKEECGKLKQKIAVFKDGKTQIENEMTSLNKIVKEMPVKRSIRSQTVRRRHRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5B2</accession>
    <name>A0A1E5R5B2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3518</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R5B2"/>
    <dbReference type="FunCoup" id="A0A1E5R5B2">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5B2"/>
    <dbReference type="InParanoid" id="A0A1E5R5B2"/>
    <dbReference type="OrthoDB" id="67317at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056552">
        <property type="entry name" value="Ribonucl_Kappa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026770">
        <property type="entry name" value="RNase_K"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31733">
        <property type="entry name" value="RIBONUCLEASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23489">
        <property type="entry name" value="V-ATPase_su_f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ82058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="85" mass="9360" checksum="4F1CD0E0E9CB22F7" modified="2017-01-18" version="1">MKPVVSSGKAWFCTVLSAFGVIILSIIGHLFKTEHEEFVGSINDPEDGPAVARSVFMAALVYLAFFLFCGSQIYMGKKKSIELRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5C2</accession>
    <name>A0A1E5R5C2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription initiation factor IIB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">General transcription factor TFIIB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3670</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Associates with TFIID-IIA (DA complex) to form TFIID-IIA-IIB (DAB-complex) which is then recognized by polymerase II.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TFIIB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5C2">
        <property type="interactions" value="838"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5C2"/>
    <dbReference type="InParanoid" id="A0A1E5R5C2"/>
    <dbReference type="OrthoDB" id="25790at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20551">
        <property type="entry name" value="CYCLIN_TFIIB_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.170:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="11">
        <location>
            <begin position="32"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="367"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="370"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ82068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="390" mass="42860" checksum="65CA821EDE461F83" modified="2017-01-18" version="1">MSSQMMNRSHASMNRAPAPGAATSDRRGPNLNIVISCPECKVYPPKVVERFSEGDIVCALCGLVLSDRIVDTRSEWRTFSNDDQNGDDPSRVGEASNPLLDGTQLTTRIGPNTVGGDLKVVRELNKTQSRSVVDKRDNDLQAAYAKINMMCDAAELPKIVKDCAKEAYKLCFEERPLKGKSQESIMASVILVGCRRAKVGRSFKEILSLTNVRKKEIYKTFNIIKNLLKEKNATTFTNIDTTNISSGQSSAETFIPRFCSHLGLSVQIANSAEYIAKHCKDINVLAGKSPITIAASAIYMSIQLFNLNIPTSQVSHTLQVTEGTIKSGYKVLYEHKDRVVDPELISSGKVSLEQLPKPNERITYVKNEEGGDGEXAGDENVNETEPVTNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5F6</accession>
    <name>A0A1E5R5F6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R5F6"/>
    <dbReference type="InParanoid" id="A0A1E5R5F6"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="297" mass="33546" checksum="5F2D2265F72783F5" modified="2017-01-18" version="1">MSETNNNATINSAGGDLKTNRISGNHPSERHVFHHDTLQTKPTSPSAKSVPKGGNNKTQGKTPNENRSNRNKNRNKSKKSLEQNAKSVNKSTDTQQRNTGMKHQNLKSIEHKRSQKRNTGPGSKHLKPITKKYDPEVELLKEKQKEELRIVKARLGPKNFQTYRSSPDFTVFAVSCMIDLNSLDLEKNASKQKPKQKRKVKFTITVPKLYPKTSITLGNILTPALGAEDSPLKNTHDWNKIVTNFNTECSRLLKEQHQPLLQQLNYFAVNFPAMCVPNKAYLKATDLKKQLIAELSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5H1</accession>
    <name>A0A1E5R5H1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3548</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R5H1"/>
    <dbReference type="InParanoid" id="A0A1E5R5H1"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="135" mass="15490" checksum="FAB1A76F8C7160CA" modified="2017-01-18" version="1">MSSNVSYGNNDQDEQLDYYTEYLENSTGDMTTKTCDNNENINNWTQQMYDRSITEQLSSVVQQKMNQPLQRNTTHLKRNREEEETDSAPHNKVSNSAHGGVTSLNSGLLSHTSGLPFQNNNMQSTYTNEKKKNRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5H3</accession>
    <name>A0A1E5R5H3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sporulation-regulated protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3626</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5H3">
        <property type="interactions" value="183"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5H3"/>
    <dbReference type="InParanoid" id="A0A1E5R5H3"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031105">
        <property type="term" value="C:septin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="7">
        <location>
            <begin position="229"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="113"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="553"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="134"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="8">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ82156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="648" mass="74249" checksum="49DFB47BAB8DF2EF" modified="2017-01-18" version="1">MTKENPIALVESVSSSFTKASITQKTDNLGSKNSVKLKEELTNNVDEFLTETHEVLKNINLKEEILTPQSAVITHLNDQSLYIGKKNTRFNSKKPLVSLKKLMDINKIDHSAETSNSKISKRDNSKXKVQSYVESKDEDKKFASDKVDKGKGTEKITPENGSKEMEKLPDGFXKKKLEVSITQVAFDKDSIDSQINEINASRTNEDLENEKTCGIETLPFQRVKIKTNKPVTYNIMCVGQSGLGKSTFINTLFQTQLLDTAEKDHANXEHEGTSPCVGTESKTTCICRKTYYLEQDDGMCIKLTCIDTPGFGDYANNQYAWGAITSYINDQYTQFFLQETQPDRAEKTDTRVHCCLYFIPPSIKGLSSLDVEAMKEIAQRVTLIPVIAKSDGLLVEETQVFLKNIRETIEKQEIKICKYLQDELDQDSFFKHVQKNIPFRVIGSSTKVLNSENKMVYGRSYRWGTCEVENPEHCDFVKLRNLLISEHLDSFFALTEQYYATWRANIVFSQNKQSQRLSFSFDLWKEIAHWNPVYXYQERELKKKYNNMTKCELQEFKNKKKILLNRQEALNEDINGLREMLFILEKECAGFEALQETAKTEEIAVENDNHFENVNENGLKPDANTDLTKLSRTNSFNTALGSFFSKRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5H6</accession>
    <name>A0A1E5R5H6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Mitochondrial inner membrane protein COX18</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3620</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXA1/ALB3/YidC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5H6">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5H6"/>
    <dbReference type="InParanoid" id="A0A1E5R5H6"/>
    <dbReference type="OrthoDB" id="2148490at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428:SF65">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX18, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="OXA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ82154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="274" mass="31788" checksum="FFD97215DFFC53BE" modified="2017-01-18" version="1">MSQTDKQNSGSPQAIPSELLNTPKHEYPETYPEIPKFITALKEKSGLSWPXFIPVSTIIVRTCITFPLSIWQRKRLIKQNELKPIVKAMSPVFKSKLAYSNNVSNHKLSPEQIILLSVKETRKRQKQLFKHCNVSMWKNIVLPLVQFPLWITISYGLRNNPLVLDHDPILTSLNNGLPLSPIVFSSVLGFLSLSNCEMYAKILKLRNHFEISQXSTNLRNSFTNISRFFAMLLTFTSFQQPELLVLYWISSQTYSFVMNLILNKWWPFEYKLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7B9</accession>
    <name>A0A1E5R7B9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Hsp70/Hsp90 co-chaperone CNS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3257</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TTC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7B9"/>
    <dbReference type="FunCoup" id="A0A1E5R7B9">
        <property type="interactions" value="1264"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7B9"/>
    <dbReference type="InParanoid" id="A0A1E5R7B9"/>
    <dbReference type="OrthoDB" id="420195at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21381">
        <property type="entry name" value="CTWD_TTC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044059">
        <property type="entry name" value="Csn1/TTC4_wheel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46035">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46035:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18972">
        <property type="entry name" value="Wheel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="70"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Cns1/TTC4 wheel" evidence="5">
        <location>
            <begin position="248"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF18972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ82810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="373" mass="42989" checksum="46301774E874C6DE" modified="2017-01-18" version="1">MSEKLKKYVPGPGDPALPPQLSEFANKTTDEVLEELNKMPFFMTEMGNEENDTLEALKAMAYEGEPHEIAANFKNQGNEMFKLKKFKDARELYTKALEIKPDPKEHSELLSILLSNRAACELELRNYRKCINDCKKSLNHNSKSVKAYYRMAKAFCLLDKLDEAKSALEFGMLIDNSNKSLYELQKKIENKEMEILAKDTEKLKMQEEKHRKTELLKASMDLRAITNVNSKAPADILKEAKIYLEEENVVDSQMIYPALILYPTTDEFDFVAEVGELSTPIDILNMIMQRPQEYFDQLGHENFSAKNMQPYMETLTGGLVKVGKKITFHNILKTEKPVIPLFDNSLRIYFVPKQESESWLAKWDKTAAMERRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7S5</accession>
    <name>A0A1E5R7S5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cyclin-dependent kinase 8</fullName>
            <ecNumber evidence="4">2.7.11.22</ecNumber>
            <ecNumber evidence="3">2.7.11.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">AWRI3579_g3243</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="4"/>
    <dbReference type="EC" id="2.7.11.23" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7S5">
        <property type="interactions" value="1122"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7S5"/>
    <dbReference type="InParanoid" id="A0A1E5R7S5"/>
    <dbReference type="OrthoDB" id="6284126at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009891">
        <property type="term" value="P:positive regulation of biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000408">
        <property type="entry name" value="Serine/threonine-protein kinase SSN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000774">
        <property type="entry name" value="Serine/threonine-protein kinase SSN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF495">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0010">Activator</keyword>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="21" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10 24" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="91"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="172"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="688"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="21">
        <location>
            <begin position="563"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="18"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="178"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="200"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="688"/>
            <end position="710"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="OEJ82965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="815" mass="92524" checksum="424CFDFFA94A27E8" modified="2017-01-18" version="1">MNGNFNNNANQVPQHITQQQQMLQQSSQTRNAYQDQYSQLQSLQQQQQQQHAHQTSQQQRQVFLMAGNNMFTIGPYKKRKDACRVSVLEKYEIIGYIAAGTYGKVYKAKGKKDALXNQESANKVNLLEISSKNHNADDFASIRNTATKKSIINRSKNNEETTITDIINKKNKLSTPVSDSSSKSADNAXNDAEKKGLKTGSVNGSSGKINLSTSGGNLGPASAELQNQKAHAKPKTQXPQFYAIKKFKTEREGVEQLHYTGISQSACREMALCRELNNKHLTELVEIFLEKKSIYMVFEFAEHDLLQIIHSHSHPEKKLIPPQMLKSIMWQVIDGVSYLHQNWILHRDLKPANIMVTVDGCIKIGDLGLARRYNNLLQTLYTGDKVVVTIWYRAPELLLGARHYTPAIDMWAVGCIFAELIGLRPLFKGEEAKIDSKKTVPFQASQFKKIIEILGTPTTQSWKDIEKFPEYPQVNNRFPHYESNLLNWYQACNGKDKWCFDLLCKLLIYNPIDRIDATAALDHPYFKNNEPPVSENVFEGLSYKYPPRRIHTSDNDIMGIGKVAQQQQQQQLLLQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQLKQHLESQAQGVFDNQVQQPQVNQTNNTFSNNLQEATLIDPNISSRASYSAPLKMRATGTAQDLTDTQVQHKQNQASSNVQSTSFHQPAPNDLYRPLGVQTASGSTKKSTDLSAQASAAAILKQQQQLQYLQQQGKNLTPQQLQRLKQMHAKKTKQNSFDKGINERIMAAAATAAAAVSKPGDTANKKRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7W8</accession>
    <name>A0A1E5R7W8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein SGT1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3412</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7W8"/>
    <dbReference type="InParanoid" id="A0A1E5R7W8"/>
    <dbReference type="OrthoDB" id="27237at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010770">
        <property type="entry name" value="Ecd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13060:SF0">
        <property type="entry name" value="PROTEIN ECDYSONELESS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13060">
        <property type="entry name" value="SGT1 PROTEIN HSGT1 SUPPRESSOR OF GCR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="390"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="534" mass="61278" checksum="8D55259473F8E3CD" modified="2017-01-18" version="1">MKLFNGNIVVHDPEWLENATEGDTRHEQREFNVLEVVEKTTAVFGLFFIDKYKVNVSKELQSVYEQLQLFLSKWNEVFPWNMYXGISTKMVKTQQSMSYILGSLCVDDNAEIEESFLVGILSQFTRQLKNDKIFIKILDTEGDFLMADCHESIPEEYEYPIATNRLWLNLGHFIMIPKDIYYPSRGLTLDESIDFLERAFFKCVKITGITDVLDTKYDENFCTNFVETKLCLVNMTLKNKXYYEYLKQNSRLFTPILKNAYASSSSPSGNTAREGTSSKNESFELALPTSSSYGDVLTMLLTSGNVSEPSLSQXIGDLVDKSLSDIIRNGDVDFTDECGEESNYKINTSYIPEFAKLPLFEPNLKFIDNLKSEENTDNILNQLNTYLEKSKLQTQEDETKNGGREQQTDEEVDEDEQARNYFEKEGTGINEDDFFEFFLKNALKLKSEDLNQFRNLENTNAYDSEVPENDTFQETDKEQEDLEEMEALMQTMMNNDKTNSSSGLNDLLASLQIDGSMPGPLQSILENLNSQFND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7X5</accession>
    <name>A0A1E5R7X5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerases I, II, and III subunit RPABC3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3393</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the eukaryotic RPB8 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7X5"/>
    <dbReference type="FunCoup" id="A0A1E5R7X5">
        <property type="interactions" value="819"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7X5"/>
    <dbReference type="InParanoid" id="A0A1E5R7X5"/>
    <dbReference type="OrthoDB" id="20018at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000191">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005570">
        <property type="entry name" value="RPABC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10917">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10917:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03870">
        <property type="entry name" value="RNA_pol_Rpb8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000779">
        <property type="entry name" value="RNA_pol_Rpb8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00658">
        <property type="entry name" value="RPOL8c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ83007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="144" mass="16391" checksum="50A6F1194F34C3CA" modified="2017-01-18" version="1">MSTLFDVVFQVEDVDGGRYRNVSRIEGKSNDCKITVDINTELFSVNKNDTLTLALASTLNNEDTTNTQIQGQATQSWRPPVPGERNLSDDYDYCMYGVAYKFEEVDQNNIAVYYSFGGLLMRLEGNPRNLSNLKQDNAYLLIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R809</accession>
    <name>A0A1E5R809_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein MUK1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3270</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R809"/>
    <dbReference type="FunCoup" id="A0A1E5R809">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R809"/>
    <dbReference type="InParanoid" id="A0A1E5R809"/>
    <dbReference type="OrthoDB" id="10264848at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00167">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109993">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VPS9" evidence="2">
        <location>
            <begin position="245"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="413"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="155"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ83045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="557" mass="62590" checksum="D040B0C38995045B" modified="2017-01-18" version="1">MTETPTNNENLNDPSAELATKMAISTSTSSTMKTSSIRLPEALNILVRYFITDLKEPKYTKPLTICQLAFLFQKFYCDFEKAIDSFQQQLRAGIPTLNTQKQHARSSSLTNIGSGLSGFFTRSRSSSNASTNHPNAPNSNVNSTSLASATTRVPLSTSASSTSELATTSLQNQNKKNGIPYDTEIDDEKLKSEYMKILERQLFERISSSGTNVPDSKVIEKFRFENLFKNNQEYLDFNTLFNSKLKNLNSLKETVDWKQFLSVDNIPDLDKLIMDLKVKELFLDFTRNSLSPIDKMETLLLLQNTIIDHLPTSGNDDLLSVLIYCTIEFSVEQLFLTYKFIKLFRCRESLKGQELFAITNIEAVIAFIGDLVDVPGMELYNIKISEQISIPENDEDDLLKNGSSLEDQELQFESKDSVDGSIPNKFSQQQQQQQQQPTLRNALNVNKSFSSLSSIKSVLGKLYYPPGASPVANTPTEPLFSSSPVASPVPVNSEIDRNVMDDDMQTSTLSSSTSKTISVDTDLCQELNCYRGKQFEDLTINELKMMFNTYQKISDQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R814</accession>
    <name>A0A1E5R814_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Magnesium-activated aldehyde dehydrogenase, cytosolic</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3273</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R814"/>
    <dbReference type="FunCoup" id="A0A1E5R814">
        <property type="interactions" value="964"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R814"/>
    <dbReference type="InParanoid" id="A0A1E5R814"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004030">
        <property type="term" value="F:aldehyde dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046394">
        <property type="term" value="P:carboxylic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000026">
        <property type="entry name" value="Aldehyde dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000012">
        <property type="entry name" value="Betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000007">
        <property type="entry name" value="NAD/NADP-dependent betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="3">
        <location>
            <begin position="42"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="4">
        <location>
            <position position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ83047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="525" mass="57385" checksum="658151493FE3C9AF" modified="2017-01-18" version="1">MSYEKAESVKITLPNGLEYEQPIGLFINGEFVKPLKENSKPDLVTENPATGEALATIYTATPQDVENCISSTYDLFEKGSPISDESAEQWKNLDPRIRTEYLYKIGDYIEKNLDLIASIECADNGKAFALAKGDVQLAAQYFKSVSSMSSMNGRVVETGDKYMNYTLREPVGVCAAIIPFNFPFLIMVWKLCPALFAGNHMIIKPASTTPLSALYFGKICQEVGIPKGVVSVLAGSGRVVGDAMSTSEKIHKLSFTGSTGVGKSVATKATESNLKKVTMELGGKSGHIVFADAIRSKDPKDINSTMENICNGIFKNAGQICSSGSRVYVQDEVYELFLSEFKKYIESTIKIGNPFNKENYQGAINNKSQYNTILNYIDIGKDEGAKILTGGKALTKTSQGEETKGYFVEPTVFYDVNEEMRIVKEEIFGPVVSISKFSTANDVIAMVNNSEFGLGAGIQTKDLNTALKVSKKLQAGTVWINTYNDFDIGVPFGGFKQSGYGKEMGVEALEAYTHVKAVRIKLDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R8V6</accession>
    <name>A0A1E5R8V6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">isoleucine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Isoleucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3241</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R8V6">
        <property type="interactions" value="741"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R8V6"/>
    <dbReference type="InParanoid" id="A0A1E5R8V6"/>
    <dbReference type="OrthoDB" id="10264412at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07960">
        <property type="entry name" value="Anticodon_Ia_Ile_BEm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.830">
        <property type="entry name" value="Ile-tRNA synthetase CP2 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02002">
        <property type="entry name" value="Ile_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033708">
        <property type="entry name" value="Anticodon_Ile_BEm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023585">
        <property type="entry name" value="Ile-tRNA-ligase_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050081">
        <property type="entry name" value="Ile-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42765:SF1">
        <property type="entry name" value="ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42765">
        <property type="entry name" value="SOLEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="8">
        <location>
            <begin position="42"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="9">
        <location>
            <begin position="755"/>
            <end position="859"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ82963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="997" mass="114459" checksum="F5FAEA9A1E2DC2BE" modified="2017-01-18" version="1">MLKHSYQQTLNLPKTKFSNRSHLQNTLNKLIPASCEHVYSQQYQELMSKKNKLQRDDVFILHDGPPYANGDIHFGHALNKILKDMINRYQLLFNQKYVWYKPGWDCHGLPIELKALAKLKLNKSDLNPLKIRSIARKHAVKQIDLQIQQFKKLGIMTNWNDRYVTLDPEYEINQLKIFEKLLKNGLXKRQKKPVYWGTETETALAEGELEYNDEHVSKSAYVKFPVVGECLIKREEYMENIATNGISNQAWGKQLTLPENTKLLIWTSTPWTLFSNRAICFNQNFEYFLITRVDDPKHEYLIVEKTLFLMNAKIFDAETYSIVNIIKGSDLSHLKYCNPLYDSPMSKIMPLLHGDHVTNNQGTGLVHTAPGHGPDDYLLGLANGLEIYSPIDDKGNYILSDPKWPAKNLFSKEAHDPANVLDPKTTEIILQSLKDKDCLLHAHDYVHSYPYDWRSKKPVIIRSTPQWFADLSEIKPLALESLQNVKFQPERGFNRLSSFVKSRNEWCISRQRYWGVPIPYFVNKNDSSDILMNSETTSHIIDLIRKKGSDYWFVESSDIEEWLPDGYKHYKDQYVKGKDTVDVWFDSGSSWQVLSFFEKFFSSGKSLSDQAKDLQADMYLEGSDQHRGWFQSSLLTKIGSSLKPVAPYKQIITHGFTLDEKGVKMSKSIGNTIAPMEMIEGNSARQLPALGVDGLRLLVASSNYSTDIVAGPLVMKHVGESLKKFRLTFRFMLGNLNGFDPRNDLLPLNQLRTLDKYILQTTTKMMQECVENYENYSFNKILTLVQYHMNNELSALYFNCIKDSLYSDSPNSLKRKQIQTTLYHVLDIYRSIMAPIIPIMVQEMWDNSPLKLEINDKTSQGSIGMGQCAFTRRPPSLAECLEAVNFETHLDILKKFNKSYLEFISEHKDITKTVKTVAKITCPTALDLNKEELMDLLQVADVELVFGGSTNGAVTVEVSSSPDKECGRCWIHNVKDSSKDLCQRCEDAVNEKKNLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA11</accession>
    <name>A0A1E5RA11_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">60S ribosomal protein L32</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3028</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RA11"/>
    <dbReference type="FunCoup" id="A0A1E5RA11">
        <property type="interactions" value="989"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA11"/>
    <dbReference type="InParanoid" id="A0A1E5RA11"/>
    <dbReference type="OrthoDB" id="268693at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00513">
        <property type="entry name" value="Ribosomal_L32_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001515">
        <property type="entry name" value="Ribosomal_eL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018263">
        <property type="entry name" value="Ribosomal_eL32_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036351">
        <property type="entry name" value="Ribosomal_eL32_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L32 AND DNA-DIRECTED RNA POLYMERASE II, SUBUNIT N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01655">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01393">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52042">
        <property type="entry name" value="Ribosomal protein L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00580">
        <property type="entry name" value="RIBOSOMAL_L32E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ83755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="131" mass="14762" checksum="14AE9F485954A264" modified="2017-01-18" version="1">MAASLPHPKIVKKHTKKFKRHHSDRYHRVSENWRKQKGIDSCVRRRFRGNISEPTIGYGSNKKTKFLSPSGHKTFLVANVKDLELLTMHTKTYAAEIKSTVSSKNRVGILSKAKALGIKVTNPKGKLALEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA18</accession>
    <name>A0A1E5RA18_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flo11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2993</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA18"/>
    <dbReference type="InParanoid" id="A0A1E5RA18"/>
    <dbReference type="OrthoDB" id="4070545at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018789">
        <property type="entry name" value="Flo11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10182">
        <property type="entry name" value="Flo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01213">
        <property type="entry name" value="Flo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51824">
        <property type="entry name" value="FLO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184687" description="Flo11 domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="781"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="domain" description="Flo11" evidence="4">
        <location>
            <begin position="17"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="678"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="678"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="701"/>
            <end position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ83742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="801" mass="82884" checksum="B9B974A656A375F9" modified="2017-01-18" version="1">MIQTLNLICFLLVLVKQAFAGKTCTPIKQCEQNNYQYFENVNSVPYYFDLTQVEYVSGNSYQITYHIWSDASIPLSSLNELKYLGSGISSSDQMIYSLNTGAGLTSDFNPTDFTMSVTVDTSSANYGSELTGLPNSFGFQFDYCKGQNTNSSPINSVFSKGDDYTCQCDWHYGATSFDYYTSCNGDNNCNSQAAFXNWLWPKECADITDEKQQISSLSSKSPQSYCTYTGATNTDWVTKITTASTGSXCTSTSSGSSSSSTSSCSTTSSSSSSSSCSTTSSSSSTSSCSTTSXSSXXSSCSTTSXSSSXSSCSTTSSSSLPSSSSTWSSTSSSTWSSTPTSSSTWSSTSSSSWSSTSSSSWSSTPTTSSTWSSTPTTSSTWSSTPTTSSSCNSTSSTWSSTSSSTWSSTPTTSSTWSSTPTTSSTWSSTPTTSSSCNSTSSTWSSTSSSTWSSTPTTSSSCNSTSSTWSSIPTTSSKWNTTSSTWSSTSKSSHSKTTTLTPTVTSSTITYVSTSTVTTECPVTSGGSTYTTATTYIVTNTITTCPVTTKEITTNIPTTETSIPIVTSSTITYVSTSTVTTECPVTSGGSTYTTATTYIVTNTITTCPVTXEGITTTVPTIETSVTETCLTCSLVPTTTQSYTVATTVTSVFDVVTGGVTYASTSVYTVSSVVAKXSEESXSTTSIAGPSPSTLTSPXNPAGSVTTEKTVSGTVTGSGEQATSSPLTSLVTVSTGSIALANTPSTLSTVSTSGGASSVGSSAAVATLNASGVQVAAAGKRHVTTFMSLLVSFITTGFYLIFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA44</accession>
    <name>A0A1E5RA44_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AP-1 complex subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3018</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA44">
        <property type="interactions" value="672"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA44"/>
    <dbReference type="InParanoid" id="A0A1E5RA44"/>
    <dbReference type="OrthoDB" id="28053at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030121">
        <property type="term" value="C:AP-1 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050840">
        <property type="entry name" value="Adaptor_Complx_Large_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017107">
        <property type="entry name" value="AP1_complex_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008152">
        <property type="entry name" value="Clathrin_a/b/g-adaptin_app_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22780">
        <property type="entry name" value="ADAPTIN, ALPHA/GAMMA/EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02883">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037094">
        <property type="entry name" value="AP1_complex_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00809">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5 8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin adaptor alpha/beta/gamma-adaptin appendage Ig-like subdomain" evidence="9">
        <location>
            <begin position="719"/>
            <end position="840"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR037094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ83785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="848" mass="96628" checksum="E9821DE03FDAD1E0" modified="2017-01-18" version="1">MGSLRHFIKDVRNASTPSKERELISKESAKIRTRLRNDQLDVTRRRVYIQKLLYLYVMGEKTHFGQVDCINLIASDNFTNKRLGYLSAMLLLKDENPELLTLLVNMINQDLQHKNDYVVAMALHALGSLCNEELARDLCGDVINLILKCGNSNSYVLKQCLSVSSSIIKKDPNLIQQYMTVENGQLITNIYSAXYGTSNTVSSLRRLSSPSTNGVLLSILEFVKSCLFQQNNYDFDTNFDELMVKNLINPMLPQWITFLKNKHASNHDPEYEVQGVNDPFLQVSIIQTLRMIFMKYNDVVSPQCLVDFKYLLNKITNSGNTPSVSNGKSSSSKKSLSVKYEAIKTVLILPSVETPLKTAAINVLLKFLISRDANHKYVALNTLINGVKYLNELDAKHLRDIFSCLYEADPSIKQRALELSFEILSNNKLVEEDFVIGELMKFLSQCNENDKNIVLYAITKLMEFGYFSNTKTGLQNLVYCIKYGGKYLSSEQINDLLISINNNNATRTSNCKFLEELIKMSMTRLASTVLEKNDDYYYIYNNYGLKLTTVWCLGEYGDIILSKFSDESQVLKYLFDISNEFYDYSINPDSFTIVNYLVTTILKLSVKFTNGGNIEKLRQLLLKYSKENDNLVIKMKSSQYLVLFVQPNDVKTQILDKMPFFERSVVSSSFGQQSVSPKQPPSKKVDLLTDLLGDMDITPKEPLKELNTEQQHSQISQKNISVPANAVKIYENEHIEIFTTFEHQSGALKSVDINLFVRLSLKYDHASSSSFSESIDNIQLFVAVSKSQKINLGHLSGHSLTVHQNSVLEQRMKIQGTGPVKTRMKIQYEVENFPFVEQFDYAFDNLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA53</accession>
    <name>A0A1E5RA53_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Protein YOP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3019</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required to generate and maintain the structure of the tubular endoplasmic reticulum network and the vacuole. Induces high curvature in membranes and causes membrane tubule formation. Involved in membrane/vesicle trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the DP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RA53"/>
    <dbReference type="FunCoup" id="A0A1E5RA53">
        <property type="interactions" value="356"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA53"/>
    <dbReference type="InParanoid" id="A0A1E5RA53"/>
    <dbReference type="OrthoDB" id="10009287at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF161">
        <property type="entry name" value="RECEPTOR EXPRESSION-ENHANCING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="39"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="163"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="163"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ83786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="212" mass="23956" checksum="59435394681C5639" modified="2017-01-18" version="1">MASPVINQVKTHMNTLDQKLAGNNLLDTFERKTSYPRSYAVIGFAVFYLILIIFNLGGMGQLLCNFIGFVVPTYNSLKALRTQTKNDDSDLLVYWVVYASFTIAEFWVNTILRWVPFYFLFKTLFLTYIAFPQFGGAQVVYQAFIQPLSERYIFKTGTTNPFSTTGAPSSNKNEQYPKPPTPPARSFENETTPINVGEEIRGTTTGFTTSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAB7</accession>
    <name>A0A1E5RAB7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquinone biosynthesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2956</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Membrane-associated protein that warps the membrane surface to access and bind aromatic isoprenes with high specificity, including ubiquinone (CoQ) isoprene intermediates and presents them directly to Coq7, therefore facilitating the Coq7-mediated hydroxylase step. Participates in the biosynthesis of coenzyme Q, also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the COQ9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAB7">
        <property type="interactions" value="251"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAB7"/>
    <dbReference type="InParanoid" id="A0A1E5RAB7"/>
    <dbReference type="OrthoDB" id="619536at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013718">
        <property type="entry name" value="COQ9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012762">
        <property type="entry name" value="Ubiq_biosynth_COQ9"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02396">
        <property type="entry name" value="diverge_rpsU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21427">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21427:SF19">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08511">
        <property type="entry name" value="COQ9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="10" id="KW-0830">Ubiquinone</keyword>
    <keyword evidence="4 8" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="COQ9 C-terminal" evidence="9">
        <location>
            <begin position="158"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ83842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="254" mass="28985" checksum="251EB0249DD95E2F" modified="2017-01-18" version="1">MFKSVASHSSIFKSRFVGQSSTAISANLLLPIFKRTYIPNSQEFPTTKHEALDSFKNIELLEHCLHXNVPQYGFNERSLVASCNDLNLSSSALSNFGVSELIRYHLVKNRLNLHELSENSTXTXTXESLFLARLKSNIPIGEQLSPMLSNLLLTPFTASEELHNLSNDFVYLSSDVEESHDFSWYSKRLGLSMAYXSSELFMAQDKSANYVETMNFARNRLRNWTECSSMYHNTTEFLWFQMLSGVNFIKSKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAE5</accession>
    <name>A0A1E5RAE5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Alpha-1,2-mannosyltransferase MNN2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3046</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAE5"/>
    <dbReference type="InParanoid" id="A0A1E5RAE5"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646:SF6">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ83880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="626" mass="72939" checksum="581E7130E80E9EC3" modified="2017-01-18" version="1">MNTKHTLRSIHSTTLIKPTSTLHTHTSVSVPSQTSESDKHKSASVEDKQKTHNVHTFFKQFFDILTKAKPTCDGLNDYNVGTCPMKSSVAYWGKDDVAKWKWLSYESLLQCLKIDESSFDDLHKNHKYFMDHFVDLYDSWESHIWDNLSASVSSSSPSPSPSSSFKTTASANPRNNALYHGNGIVVVGGGKYSVLSLLLIEALKSQNVHLPIEILIPPAEASLEKEYCEFLENNHNRYNAKCIYFDRVFPQELLENFEFKGYQYKSVALLASSFENVLLLDADNVPLTDISNVFQSHVYKKFGMILWPDIWRRSTNPKYYDIAEIKINFEKRTANLIDRFTPAQVYTPQDKLLDTEYLRQEVPYHDFEGAIPDVSSETGQLLINKRTHMKSILLSFYYNVYGPEYYYPMFTQNMAGQGDKETFVAAAHVLNESYYQVKSTAGIAGYHRQDKSGFRSVAIYQKNFIQDYDLYEISKRKTYEKYDIKDVNKGKWIFRNEAQKEQSIAKYNPEYSPQTSYLNLFFKNKETEAQNMFAHCNFPKFDPLEMSKKKDFTWEGKHFRVFKRQRVNGFDVEKSFYTTFDTHLCQSNGRVEFSYLNNKIXDETEWNEMCQFIHEKLDFLQENKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAJ1</accession>
    <name>A0A1E5RAJ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein ERP5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2711</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAJ1">
        <property type="interactions" value="269"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAJ1"/>
    <dbReference type="InParanoid" id="A0A1E5RAJ1"/>
    <dbReference type="OrthoDB" id="3427at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="9">
        <location>
            <begin position="62"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ83925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="248" mass="28820" checksum="666D390CED16AFA4" modified="2017-01-18" version="1">MKESVKKQQGKYHWKRFAIERFELDRTIMFAKSSLLSTTLTLLGLFTVFSQATHVYLDLDKPKCFYEELSQDMILNGQFLAEFGSNAEGYVNDDTLQLFVEITETFDNDQKVLSQKSTNNGQFTFTALESGEHAICITPKKLGKREKNSENIRLHIELNRGPIAAVDVTQKTKXNRLKTRIDQMGSKLSNIKAEQRVIMDREARFRDLSEKANNQISWWATVQVLFFCGTCYFQIRYLKNFFVKQKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAP1</accession>
    <name>A0A1E5RAP1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2902</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAP1"/>
    <dbReference type="InParanoid" id="A0A1E5RAP1"/>
    <dbReference type="OrthoDB" id="5398391at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3330.10">
        <property type="entry name" value="Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018020">
        <property type="entry name" value="OHCU_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036778">
        <property type="entry name" value="OHCU_decarboxylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37987">
        <property type="entry name" value="CHROMOSOME 9, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37987:SF1">
        <property type="entry name" value="OXO-4-HYDROXY-4-CARBOXY-5-UREIDOIMIDAZOLINE DECARBOXYLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09349">
        <property type="entry name" value="OHCU_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158694">
        <property type="entry name" value="UraD-Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase" evidence="2">
        <location>
            <begin position="17"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ83975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="206" mass="23477" checksum="F0A5BFAFBE53164C" modified="2017-01-18" version="1">MNTMSLPLSFGDFREKASLDEQIAFLDTLFEPTDSLIYFTLKNQLFQKRLATQVHSYDGLIELIRKTMLDLNTAAEEEGISSFKIDHLANIIGAHPRLGEPPKQLSVHSLSEQKNLGAGSNSPDDMETMKMKNELMQLNDQYEVAYPGLRFISFVNGRSRPEIMAEMRVRISSGHTWFQEVAIGINAMCDIALDRLHKYTDYTQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAS7</accession>
    <name>A0A1E5RAS7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">D-arabinose dehydrogenase [NAD(P)+] heavy chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2598</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAS7"/>
    <dbReference type="FunCoup" id="A0A1E5RAS7">
        <property type="interactions" value="159"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAS7"/>
    <dbReference type="InParanoid" id="A0A1E5RAS7"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="5">
        <location>
            <begin position="24"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="4">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ83997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="305" mass="34984" checksum="E045B9C7D62232D6" modified="2017-01-18" version="1">MSEKLNATKVNYKLNNGKTMPALGFGTFCAPEDQSIVKDCVIAAVKAGYRHIDTAYLYQDEKAIGDALQELFKEGVVKREELFITTKVWPNFWDKVEESFSTSLKNLQLEYVDLVLQHWPLCYPKITDNETGYIVFTPTDDQGNIMEEKDASYLETYKQLEKIYLDENDHRIRSIGVSNYTVDNLKDLFRDPTIQTCPSVNQIAIHPGKPQNEIVDYCQEKGIVVTGYTPYGSQGGPALKLPLIKQLSEKLDMSVNEVVISYFLNRNLVIIPRTWKTERLPQSLHYKKLPKEIIEQISNVKIDFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB72</accession>
    <name>A0A1E5RB72_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RB72"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="321"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="187"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="361"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="375" mass="42059" checksum="9493BAE2A489C87C" modified="2017-01-18" version="1">MIYRLIYLPNSHYIPNSSKEENEEEEKVREKGEYGGYDENEVLHNPFERLCENFAREQYRYDMHYQKELRRYAKSMPHKGDHRQVFPKKKKRKRVDHMGEDVTPLSKTVAADTAHSIDHNGKSLSESSSANSVITKDGGLLATPLLSVSEGTTYTPYTKGNFYNENGREETVVDENENENENENENGNENGNQNYDNESWSYSVDASSLMSLKRRPSTIASATTIYSNNNYNTPTKNIGGIGCINTPNSMDKTNMNDTNKASLNGSDPMSIHYNHLQRTPMGFPQHSPSNHTQIIGRKDSMAMSLSKRLEELSQFHVQSGALQKGPDVSPNSNGNGANGSGIQIPSVHMYSIPNPLQEEYTANNTSNNDXTSSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBC7</accession>
    <name>A0A1E5RBC7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcription factor CSR2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3048</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBC7">
        <property type="interactions" value="125"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBC7"/>
    <dbReference type="InParanoid" id="A0A1E5RBC7"/>
    <dbReference type="OrthoDB" id="2333384at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="2">
        <location>
            <begin position="601"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="11"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="480"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="789"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1001"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="480"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="495"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="795"/>
            <end position="808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1097" mass="122492" checksum="D405920B331A1E75" modified="2017-01-18" version="1">MPDLLHLFHKDKESSGQRDQSHLHHGHMFSRSATSPQAQASASHAYGSHFTQRNRPSNHAHTSPLEMSRSLPRQSRTESDMYMQQGNVLVSDGLSYNTAMTNANHHNNEVYTNTLDNARLATSLNSQRVPLARDTLPMSLGYAQQSPNEHLFAQHRLSEYNDAEEEPSFLLDKTLVSYLQGRGLLKPQRISSTKELPNCPLDIFVASQGENVFLPTTSLQDEEYLQQLNSMNEHRGEPSLSNDGATLNSSATRASFGSNQNDASSTSLNRLQDQEEHATEEQIMQDLENSTVPQKFAVIVSLKARTPIKKVQVKLTGDALVYWYNGVPPKRSSSKEFYKIGETEWDLDMSKYGAYXNQEGVIKETNENNIPISIITNKPIDTVKKEPYLPLGYTRKQIFLHELLKENSNAKFAKFYEPGNYIFFLPMYFANSSTESIYVPSARIKHTLHCGVMISNTLYAKELANKSGVNTINNSLAGSVHMQSPLSHSPRSMKETNSSTSLLSQNSSLEESTPPGHSGSATSGGGFFKKFIGSSSATSHGQLSHHHSXNASSQISLPQDLHTFYNKWGLIYGDKLINLVRIAPLRSVSTADKPIYINRVWNKSLAYEVSLPEKYIPLGSVMPIRIKLTPIDKTLSIKRIRVGVVEKITLMSKNMEHEFDQLEPLLADPQNPYYQEFIKKRKTDRCLSILEVRACNNTGHPSMKEITVSNCKKDNLFQFYDKNIDAFIVNCNVEFPKFEPLSVLKSKLNLKYGPLKSLAPYGCDKYYEMLPGDDDVLKEQEDTENLALHRKNSANRDSSPHSAGSSAHDSSKERRGSTAGSILSGIFGKRSRHNSVHHESANKECPDANIVDFLENQSHTHTIEDSLFSQSGVKVQFDTTLKHSERGLYTDSVNSNNVHTKHKLEILLRLSKYDETLGKSRHFEVIIDTPICLLSELCVDDNVSLPNYTTAMMNSIVRDVENDESAPPPTFEEAISVPASPVGSPTMHAINKNLFDDDNSISNLSLTDSSNGTETPTNGQALPPNQLSHTTSNLAPHTDNFNNIDDLLHSHSDTALHNQILNAVPQNKNYPNYTRNPRDMLPSYNDSVSMAGEAGYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBG4</accession>
    <name>A0A1E5RBG4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">High-affinity glucose transporter SNF3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2895</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBG4">
        <property type="interactions" value="267"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBG4"/>
    <dbReference type="InParanoid" id="A0A1E5RBG4"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF16">
        <property type="entry name" value="HIGH GLUCOSE SENSOR RGT2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="386"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="435"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="463"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="486"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="522"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="554"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="647"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="682"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="711"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="744"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="778"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="820"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="390"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="32"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="887"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1012"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1199"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="32"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="65"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="104"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="887"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="898"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="920"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1012"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1215"/>
            <end position="1230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ84240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1244" mass="138125" checksum="3B20DFF7B3C6A466" modified="2017-01-18" version="1">MIGSDTNGSALTKQQSGSEYNTLSTLSNTYTHTNSHLGPINNNSCTRTNSSANYYRNNMHQDIGHNNNLDSHHNNNNNNNHNHHHHHHHXTHSMRSRSAKKSYNRSIANSSHSRTTSDIILRQKPAYIITQLLKRRKRQDNGYKLFQQQRQLIQNQQRQKSSFQNNIIENKVEKSSIESQGEEHDDVYNDTKQNXYVSTVPVISTDQAATQFELCHLPENNAHYDYDLEKKAARVSKYETGVQRLIPDAFNTQNKITNMEADLKNNIDNKTNLNKIGITQLDKYSTCGNDNNDNNLDYTSSNGNVFTNYDEILPYLHLSTTVSHGSSVSSKDRQRGSFDNKDKGFGVDRKYLSNEYPGGHRFSQDQDSYLDPKIFDKEPQNQSLKMSFLVGIFVAVGGFLYGYDTGLINSITDMPYVTKNFTTTASHEFTNGQHAIIVSSLSIGTFFGALLAPLLCDRYGRRPTVMVATFGVFMVGNTLQICSKGIPLFCCGRVVCGLGVGLISAVVPLYQAETVHKSVRGAIICCYQWAITWGLLVSSAVSQGTRLIKGSASLRIPLGLQFVWATLIGIGMYFLPESPRYYVLKDQLNDAAASLSYLRGVPIDDTGLLEELVEVKANYDYEMSFHSKSILDCFKSSPTRYKQTSRIFTGIAIQAFQQFSGINFIFYYGVNFFARTGIQNSYMISFITYAVNVAFNIPGLFLVDLIGRRKLLIGGAILMFLGNIIVAIIGTVVGDTVRSAKIRIPFICLFIASFSATWGGGVWVVSAEMFPLGVRSKCASXSAASNWLANVICAAITPYIVSGEISDSTPYAHSTGSKIFFIWAALNLCGAVIVFFSVYETSGLTLEEIDEVYKQSPCAYKSAKVNKEIKLDDSHLIGFLSLAKNYNNNNQHHNSSSAKKKKNKKKKNVRGKXFGKNSPDDNATLNSGTTHVDNSNVNSDKTNSGNTLNDSQGYRRLGPVDFEKDCKKSDXFLSKSKSNSSSLGSNSKPIGVSLEDITADKNSMAKFKTDPLTVERHDNKTQESEFQSSPQIGSKHFVDLGHGLGLNTHNRGPPSIVSEDSDFGNTFELQPNLISNLVXPQSLVVSGDDATKRDPPPEKGIDSAKRVLESXKSFIHNDVDLDDASIADSGVQADGSAYNNNIKEYMAQLVDPLNNVHSCDIQEKQVDNLEQLECFRNSPLNYTEMFARVRQEPPSVFLSTHDDSDGVEGLVNDFDFSEDDDDDDDDDDXEPPLRSQIDFSKIIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBK8</accession>
    <name>A0A1E5RBK8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MIOREX complex component 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBK8"/>
    <dbReference type="FunCoup" id="A0A1E5RBK8">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBK8"/>
    <dbReference type="InParanoid" id="A0A1E5RBK8"/>
    <dbReference type="OrthoDB" id="506431at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052061">
        <property type="entry name" value="PTE-AB_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47260:SF4">
        <property type="entry name" value="MIOREX COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47260">
        <property type="entry name" value="UPF0644 PROTEIN PB2B4.06"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="277" mass="31382" checksum="7F54DDA28288CF9F" modified="2017-01-18" version="1">MGVFNKLSAYVLIPTLGITTGIFTFIKPWPQVGPKKPGETETEAFERQDVLQRLKPHLQTYHDNPDLYEISSQRKYIPLPHQHHHIGTSLLNKEDHIEIDPLVIRNKSILNSKELQNPGKSNINDANVFANDVELTAYFHLGKELSDPKTGNIYKGIVALLMDELLCVAGFPKLPHQRGVTARLDIEHVGDIPKDTTLVLNCKIKECKGRRCEIEGTLSKVEDPAARRNWFGIGKIASHIPYVSSFFTPSPTVYARGTCLLVEPKWFKYLTWINIFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBR9</accession>
    <name>A0A1E5RBR9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein YIP4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2813</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YIP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBR9">
        <property type="interactions" value="128"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBR9"/>
    <dbReference type="InParanoid" id="A0A1E5RBR9"/>
    <dbReference type="OrthoDB" id="411251at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045231">
        <property type="entry name" value="Yip1/4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236">
        <property type="entry name" value="GOLGI MEMBRANE PROTEIN YIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236:SF1">
        <property type="entry name" value="PROTEIN YIPF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="20"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ84023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="272" mass="30642" checksum="471CB2FEA6ECBEEF" modified="2017-01-18" version="1">MDKDTLIESDIPQRGSQPAYSSPNYSSNTNTYPQSXASPIYNESHVSNVVPDTIDYDMSAHSARGTLDESVWDTIKRDLYTIHKRLNVIVYPKFVQSYITMEASELEQNGQTGRSGEANDSDLWAPLIFILLFSEISSYSSTSFSIYFVTSWLVILLGSIHLKKFSMLQSPSSSASPSTKKILMNVSLLGYCIFPILVDSIIIRLILNLVLFKYISIPALQILVKVVLISLTTFWSFYCCVVSVTTESSSIEFLHKLPLLLVFTFLNWLNMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDJ8</accession>
    <name>A0A1E5RDJ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein HRI1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1793</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HRI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDJ8"/>
    <dbReference type="FunCoup" id="A0A1E5RDJ8">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDJ8"/>
    <dbReference type="InParanoid" id="A0A1E5RDJ8"/>
    <dbReference type="OrthoDB" id="4045395at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd11693">
        <property type="entry name" value="HRI1_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11692">
        <property type="entry name" value="HRI1_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.310">
        <property type="entry name" value="Protein HRI1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.320">
        <property type="entry name" value="Protein HRI1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031818">
        <property type="entry name" value="Hri1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038744">
        <property type="entry name" value="Hri1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043047">
        <property type="entry name" value="Hri1_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16815">
        <property type="entry name" value="HRI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ84975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="237" mass="26673" checksum="ECEE502EF69584F6" modified="2017-01-18" version="1">MVQLFQRKLFQVLPNPPCETTSTFSSVTNAGHFISLRPFVNKAGHEDFPFEWCFGGISKYSQASQLDADTAQIIFTMELDTNTIRKVENVDKDPVTTKWATWPSGDVEETGEVFPFGKDKEGVKFRELWQPINPDTYETEQKFNILPRSADASKFASIALYVNNSEFHGLVTIVGNFVQGFLHKKGTESMSFIRAAFNGSKYEYSIKFGPNVDVFPTKFHNGVKVGEKVGVFEVIEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDR4</accession>
    <name>A0A1E5RDR4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Ubiquitin-conjugating enzyme E2 2</fullName>
            <ecNumber evidence="3">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">E2 ubiquitin-conjugating enzyme 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19 21">ubiquitin carrier protein UBC2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17 22">ubiquitin-protein ligase UBC2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">AWRI3579_g1781</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="20">Protein modification.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="24">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="27">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDR4"/>
    <dbReference type="FunCoup" id="A0A1E5RDR4">
        <property type="interactions" value="812"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDR4"/>
    <dbReference type="InParanoid" id="A0A1E5RDR4"/>
    <dbReference type="OrthoDB" id="9984419at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23790">
        <property type="entry name" value="UBCc_UBE2A_2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000007">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 24" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 24" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0749">Sporulation</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 24" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="26">
        <location>
            <begin position="4"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="25">
        <location>
            <begin position="151"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="23">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00039884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00043952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00075663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00080892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="OEJ85021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="168" mass="19345" checksum="B45AA54675B10836" modified="2017-01-18" version="1">MSTPARRRLMRDFKRMNEDSPPGVTASPLSDNVMMWNAMIIGPPETPYEDGTFRLALQFDEDYPNKPPHVKFISEMFHPNVYANGEICLDILQNRWTPTYDVASILTSIQSLFNDPNPASPANVEAATLFKDHKTQYTKRVKETVEKSWEDDLEDLEDDEDDEDDEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDR5</accession>
    <name>A0A1E5RDR5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative hydrolase NIT3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2343</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDR5"/>
    <dbReference type="FunCoup" id="A0A1E5RDR5">
        <property type="interactions" value="646"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDR5"/>
    <dbReference type="InParanoid" id="A0A1E5RDR5"/>
    <dbReference type="OrthoDB" id="10250282at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050152">
        <property type="term" value="F:omega-amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006528">
        <property type="term" value="P:asparagine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006107">
        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07572">
        <property type="entry name" value="nit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045254">
        <property type="entry name" value="Nit1/2_C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001110">
        <property type="entry name" value="UPF0012_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088">
        <property type="entry name" value="NITRILASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088:SF30">
        <property type="entry name" value="OMEGA-AMIDASE NIT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01227">
        <property type="entry name" value="UPF0012"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="2">
        <location>
            <begin position="8"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="295" mass="32971" checksum="6A45C1534558E1D5" modified="2017-01-18" version="1">MNVLKGKVNLSLIQLAGSLPDKLTNLQNAKKLIDKCILQYPKTQVVVLPECFNSPYSVDKFREYSEIISLDEPKSISVHSMSQWAREHKILLIGGSIPELDPVDNKVYNTSIIFSPQGEILGKHRKMHLFDVDIPNGITFKESITLTGGSDITILESKLPSTTTEFKFGVGICYDMRFPELAMISARKGACCMIYPSAFNTVTGPMHWHMLARARSIDNEMYTILCSPARSADPSVYQAYGHSLVCSPKGEILVEAGESQEILNCELDGDVIDTIRMSIPINFQRRFDVYKNVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDR9</accession>
    <name>A0A1E5RDR9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidant-induced cell-cycle arrest protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2315</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDR9">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDR9"/>
    <dbReference type="InParanoid" id="A0A1E5RDR9"/>
    <dbReference type="OrthoDB" id="3971231at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="520"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="698"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="791"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="520"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="791"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="844"/>
            <end position="859"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="953" mass="109426" checksum="2038303DB3D3BE21" modified="2017-01-18" version="1">MSKPHYSRNNSNNSLPHSASVHSHTGPRHSMHASKNHHHHHQRLKHYQNSVEFKKELVEHCLDLIEQNDHNSLAYLARTCGIPPNLRFIVWPMLLKYHPLVIAPNIMSNTLQFNINYDESNSANSNNSIKENCKTTNENTKKINKVSVQNADEGCYISDDENEDITWEYHKEEKTDDEIKELIKLDVAKYFMVKYNNLEFDENVITTSILNFLHKWGKIFKYENGLTWACLSLCEWVSPVFDPESSEVLVLPGRSFHRNNLNTSQASENSTAAVPDPDGNKGSSPNGPKVSAFNTNTDQDHKSGDAGLNEQHYHHQXQHHHHHHHKHNHRHHTIQNNDIYIDLLKEYPLSKELQDRIHKIRKLHSARTTHLKFSDLYERLMLVILHSPDIKTIVENKKSKHNTLYTPLISGGDFNYNHSVFYKIFETCLNELYQPFTDQLINTRNKWLYYWLKFSSVRTFHKVDRGRFWDMLLGWRPNPDNLLFYLKYNNKYSYGHFYSSNVPASWTVKNGKSAGLVKXEEKIDQQGRDADNEGSSEQENDKKSQXNAKDSSQQDMSFWDKLFKYCNNNEDYFWFPDLANMKLNKDDADIKIFQELMKRNSLSWEKNGEPTEHHPKLHKLQGIEPHHEKDSLKYPYSXLDIHTQQLFIYLSILQKNEFKLLEFEEAEIVEFLNNVPILGKNDDSMYRNLYXLSHSKSSESEHSGALPHGISLSSPPXSDAAPSTFFPTTLSSVLGRQLHPSSSHSKASSTDDYFGEFSDVDSLDFPNTADASFYXSSTGTPTTLPTLAAVKSQSQAKQHFGESNTPSRDLDPQQNSFGAADTRKINTRGSSSDNSKKSNNEDLIXLEEGEEANREDEEAVSMQHHHFHQPNLLFSPPPTLPSSPQTLPLSPVSSVATLPLTRSSTAATTSAPSKNKIEMGTDDKLLLSFNEILNTAGDIWRKWLWSELEQNSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDS6</accession>
    <name>A0A1E5RDS6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Pre-mRNA-processing protein 45</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2221</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the SNW family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDS6">
        <property type="interactions" value="1005"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDS6"/>
    <dbReference type="InParanoid" id="A0A1E5RDS6"/>
    <dbReference type="OrthoDB" id="666364at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017862">
        <property type="entry name" value="SKI-int_prot_SKIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004015">
        <property type="entry name" value="SKI-int_prot_SKIP_SNW-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12096">
        <property type="entry name" value="NUCLEAR PROTEIN SKIP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02731">
        <property type="entry name" value="SKIP_SNW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="SKI-interacting protein SKIP SNW" evidence="5">
        <location>
            <begin position="102"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="255"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="271"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ85036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="426" mass="49061" checksum="8678F28369D64012" modified="2017-01-18" version="1">MISXSAEIISKRSEEPEDTDHEKDDPILQYQKNLLPLRLANPDYKCPLPTEDIIKESAVATKKVIEKLLGQSGPISSTAISSSATRLNNSKNFSHSIPNIFNDNRAKTXVKVIERHQDPLAPSTKKILKKIPLADDNTELLETPILQNDKGIGHKVSEKEKALLNIPSAVSSWENSKGFSVSIDKRLQYNQSDKKNAPKEINDKFVELTKALDATSEKKRIELAQRNERLLKVKEEQEEIERIKGKISEIKNFEQRQKETQRYSRYNQQNKXERPAQGDRREAIRAQRRKEIQHTSRXKNMPKKAPASTLEQRDMQXASGSLDEEGEKEVQYDSRFFTRAADASWKRSDDQMYDTPLFHQRNETASSIYRNRRSAQRTERGAENDVVNLRDTPVEFVGTEAKDETTLHLDREFHFPNKNKKRKPND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDV7</accession>
    <name>A0A1E5RDV7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="10">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2368</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the phage and mitochondrial RNA polymerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="10"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDV7">
        <property type="interactions" value="278"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDV7"/>
    <dbReference type="InParanoid" id="A0A1E5RDV7"/>
    <dbReference type="OrthoDB" id="3971271at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034245">
        <property type="term" value="C:mitochondrial DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001018">
        <property type="term" value="F:mitochondrial promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006390">
        <property type="term" value="P:mitochondrial transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1320.10:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000041">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.280:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1320.10">
        <property type="entry name" value="DNA-directed RNA polymerase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024075">
        <property type="entry name" value="DNA-dir_RNA_pol_helix_hairp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046950">
        <property type="entry name" value="DNA-dir_Rpol_C_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002092">
        <property type="entry name" value="DNA-dir_Rpol_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037159">
        <property type="entry name" value="RNA_POL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029262">
        <property type="entry name" value="RPOL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00940">
        <property type="entry name" value="RNA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14700">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01311">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00900">
        <property type="entry name" value="RNA_POL_PHAGE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00489">
        <property type="entry name" value="RNA_POL_PHAGE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase N-terminal" evidence="11">
        <location>
            <begin position="414"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="12">
        <location>
            <position position="891"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ85091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1354" mass="153211" checksum="0749CAD4E450DF49" modified="2017-01-18" version="1">MLRHKSTHLLRNTPKASARVSVSLKALNNFFPYARPSKHLVSKGGVISQKRNFNNTTNKKTPLYGSPSATDPAPGFDVLAEAYLNSSSLTSADASLRSYQYQDKSNSRELMNLLSLLDSALHLNMFQRSEAILESLYLIKSHRSQFVNDYNKYLFRLAETGDGTGRIYSFQELRNVLIADFEKKYKGITPNEKTYAILIHQAITTRNPDFDKYAFKRSIQQLLEQSYSTKKLMSNVDVLTLEDYTVLVHDLGLVDLRQIPTDLRELVVYSNNKESLSIGQHLSADADNISQTQNVGSSSEEVKKIGNFSANNELLSTSGQNEKIDFARDEVKTLEKEGLDELKSVGTVGMKAVRKSLLGMGISPNLKKRFEQIMSESLSGNHEAPMNFFEFYQKLETPEQKALFDEILDDINQERQSNLEKTASEAAKARWEFEFNESLKRGDMIGLKSMNSLLWKWYSEMLPIVKKEVELCQAALASANKNETTTANAKSTDQERNAYGIYMTLVRPENMCTITILELLKLHSTGGINEGMRLSRAVISVAKSIEMEYTSTKLAEREKKQFKLATKDSVQIRKLLHKARAAFDKNDSQTNQVIWPQDIRAKIGSILISILMQVAKIQVTATDPVTKEKVTSESPAFHHTYQYFSGSKLGVLKLHKELITKLGVENLVSAVQPQLLPMLIKPKKWETWNSGGYLYTKSKLVRAKECPEQIDYLRAVSEKGIINNVFKAVNVLGETAWTINKKVFEVMSQVWNTGEEFLEIPKSQXMLVLPPKPDSSSDPLIFRRYKLDNAVIAHTFQKNRSMRCDANYKLEIARGFLGEKFYFPHNLDFRGRAYPIAPHLNHLGNDLSRGLLLFWEGRPLGPNGLNWLKIHLANLFGFDKAPLDARVQYVDDRLDMVKKCAENPLEKDSWWQTADKPWQALSTIFEINEALKCADPEKFVSHQPVHQDGTCNGLQHYAALGGDLEGATQVNLVPGEKPQDVYSHVASLVNARVDKEAAEGNENALKVKGHITRKVVKQTVMTNVYGVTLIGARAQVFKQLTNVFSDRTEATTLSNYVTTHVFASIRELFHSAHLIQDWLADCAKIVSKSVAIPLAEDKAFKSFTQPLNMSSVIWTTPLGLPIVQPYRKVAKKQIFTAIQSVYISDPLSLNPVDARKQKMGFPPNFIHSLDATHMLLSAIKCGEAGLRFASVHDSYWTHACDVEKMNKILRQEFINLHEVDLIERLKNEFDTRYKDNIYMIKIIRKSPLGKELLEYRADLQAKLNRVPTFMDEIYLERVRRDYLNSDDPELVAKAKSMKTVLTICSSFTAQEIEEFTTKAKTSGVVGFLPVLIPPIPPKGEFDVTELRNSTYFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE75</accession>
    <name>A0A1E5RE75_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanine nucleotide exchange factor LTE1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g2234</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the LTE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE75">
        <property type="interactions" value="224"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE75"/>
    <dbReference type="InParanoid" id="A0A1E5RE75"/>
    <dbReference type="OrthoDB" id="10254377at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005933">
        <property type="term" value="C:cellular bud"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.840.10:FF:000019">
        <property type="entry name" value="Guanine nucleotide exchange factor LTE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF363">
        <property type="entry name" value="PROTEIN SON OF SEVENLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="13">
        <location>
            <begin position="87"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="12">
        <location>
            <begin position="1688"/>
            <end position="1929"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="372"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="471"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="587"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="729"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="905"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1042"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1092"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1137"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1318"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1567"/>
            <end position="1602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="483"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="495"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="621"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="905"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1092"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1106"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1318"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1337"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1355"/>
            <end position="1366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1378"/>
            <end position="1423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1567"/>
            <end position="1577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1578"/>
            <end position="1590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ85218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1934" mass="218012" checksum="CDF66EC6AD0AFF1E" modified="2017-01-18" version="1">MAAVPARNALSDRNKNHFLSSIRNSSTTKEEYESEIERELDHYKIYYPIFEDSNYYPKPTKNVMEIVPSGNNASTNSSTNTISHDSGKYSIIKSTVEGILIQFSNPIDPIDYQQFQDFFIIYRVFLDPLKLLDICLSRLKWCIDKIQEIIKENKLSYFPSTNTMDTDNDNTTNSLKYKTPIPFTIDKMEVILIRTFVLLRHWILNYYIQDFMSNKALSLKFIDFINFFDYNTTLKRKIVLNCLKNLKKSWIDQYNKMFDDAEHKINVNHLHSEKDWLNFSVPVDSKEALGGSKNVDDIGFDTNKQKRMSFYALQSSSNPAFRNQSLLSTYGAQQVFKIPQQENNLGANKFSKNPKRQSKTFNILFPQDHNNAFSNQKKISNGTSDTSINSDQELHHANSSKRQTYSFNEYTNNSVNSSIIGNIIQSKNSHVLKHLNTNYPESSAISKLAPLTPAKNLEFTISLDNLSKNSDFNEETATKNDSRASVISTGSQEKISNINNNDNNDNNDNNDNSSNTIGMLENQQVKRFVKYVFSIGTINLPNNDGSENGNEFPEKKFDILGARSIDQVQYLIDMETYFLNQLKEKTTPRNSLSGNRDILDDGSITDAFPNSSGMXKKDSTQETPDNVNGMDNLNLYQTVTSIATSVLSSARKNQVDLNESFLTSSLTKNFQLDHHSQGHGNNETVKNAHNVGEENQIPNHLRVSPFLRSLDKSNVYNHKRVSSSFFTNNLDTPSKNSKLKTNSATLKLRPLNSNDNSTRNSFDNDSNTANTTIDNNSPIHSTTVSVRSSLKKNQGPLLSDSAYDLSKHISSITSNGETERNSNLSGSINLSNEFNIMRESNXSSAATDNKQHRNILQSSLPGIPNXXAQNNKLSSINTNHLSKISKNSSSATYQPKALNSGRISISAXPLSARPSRVASSSSTSHRRSPTFGNQFDKGHKKTKSSEARYGNLLRNDLSSHTSGKVRHKSADFNNVEHNRVHQISRYPSVFANPSFVSKDQFFEEQALEVKILQRKTSRITLRDTKNQERESITEEFNASNLQAPSSHLLNHGIPQGTVSSEEQTLXAGSNQFSKLNGIPQIIEYNEHNSERSFSDAXATKDAEQASFSGSSGSDKSFSTLASVNTEMLMESPNKFDKMGRSTPNQAXAEGXQAENNSGKKNSLAILADRPVKLTESKRFTSSVSPGGPFLNDAPNVNRISTYISDSDEDLGFHMDDTSLFTDGESQKKEKRNTFNEANAKLDLRKQFHQQHDHSQDPKNKIHAATAARYSYMVYGDNPPEQMPLGVQQHQPKLYGAVRDDEYNNSFKHNFQQELDSNSSSLDFSSIHSSPEHFDSINGISNNANQSQQNRMSTHSSPSKSQKNNPKLYCENGLEKTSRPLSTRSSQVSKTLSPSKTNNPYFVVTSTSYEDQQSQRKTISSIKSDSTREKNXNFTASAVINGKHYSAVSANSPSPSKAELLRQKYLSVQHSASSLSALNLQKSNESFDDTRNITEASITENSNVGEKKSHSLQSNPKMLELANLPDKSFXGVDPVDAAMRKLEGAYSTSNDSSIKLKSILKDFNKTESNGSLLQNEGKQTNEKTDLEDSKEAQNSSLKKRDDKKRQTVLIEARRYTRLFNVFDQSQRASKSLLTQEHHESALQEKENIKIDGILVNEQITDLLKHYQLTDPNLLLKNSDEHIPFILMYSSYDIASQLTLIEQDVLNEIDWFELLELDMQTDLPNFTSWLQVLFQNEKLSGIDLSIARFNLMVDWIISEIVLTADTKMKRNTLQKFIHIAEHCIKLQNFNTVMQXVLALGSPLVQSYANSWRLIDPGDLLTWAELQKTFSVENNYSEVREFMNELDPIHGCLPFVVLYLGDLISLKDMPNHFHSNSQIINYSKFDNLVNIIKSFIQRVQWGTEFYQIKTIPQLLSKCVYISCLSAEEIEWVASTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REK4</accession>
    <name>A0A1E5REK4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1841</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5REK4"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="254" mass="28802" checksum="55DB993143AD7958" modified="2017-01-18" version="1">MTTRTTYYRIYKSNKLIRVRNNPLSTKEQPVYSIDENKHSETTSVHSFVLSSSHSSLSSSSSSICLSSEANKLSQNETIKLDQANTSHILLQNGIVKSECEKEPKSSGNISLLSNRKIDEEDGKAASNSIPHSLHSKLQNSQKSLHQKQVQNSXVLTKKKFETLQQVHXNMQLNKLQSDINSLISAFRDKINSFKTDFAMQDEKTKTARLPIXAKSNEIPDNDENDDDTEYAPKCQHKSEIDLALFYQYLNEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RER1</accession>
    <name>A0A1E5RER1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1709</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RER1"/>
    <dbReference type="FunCoup" id="A0A1E5RER1">
        <property type="interactions" value="95"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RER1"/>
    <dbReference type="InParanoid" id="A0A1E5RER1"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072380">
        <property type="term" value="C:TRC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006620">
        <property type="term" value="P:post-translational protein targeting to endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047150">
        <property type="entry name" value="SGT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45831">
        <property type="entry name" value="LD24721P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45831:SF2">
        <property type="entry name" value="LD24721P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="4"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="90"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="100"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="152" mass="16517" checksum="E974BD7485AEDAFA" modified="2017-01-18" version="1">MTSFEEYKNLGNTHYQANEYDLACTYYEKALAVDSTNAVGHSNLAMALIKLGKHCDALAQCELGLQCKPSDKIEQKLLWRMSTAREELFKQSECGTNSKTNPGTNPGTNPGTNPGTNPGTNPGTNPGGHKTVDNNTVEIPIVEVDSLPLKYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF12</accession>
    <name>A0A1E5RF12_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">cystathionine gamma-lyase</fullName>
            <ecNumber evidence="4">4.4.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Gamma-cystathionase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2255</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.1" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF12"/>
    <dbReference type="FunCoup" id="A0A1E5RF12">
        <property type="interactions" value="477"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF12"/>
    <dbReference type="InParanoid" id="A0A1E5RF12"/>
    <dbReference type="OrthoDB" id="3512640at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004123">
        <property type="term" value="F:cystathionine gamma-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019343">
        <property type="term" value="P:cysteine biosynthetic process via cystathionine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000043">
        <property type="entry name" value="Cystathionine gamma-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000008">
        <property type="entry name" value="Cystathionine gamma-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054542">
        <property type="entry name" value="Cys_met_metab_PP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808:SF15">
        <property type="entry name" value="CYSTATHIONINE GAMMA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="TRANS-SULFURATION ENZYME FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 8 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="8">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="392" mass="42064" checksum="39B0755C32FEC9D2" modified="2017-01-18" version="1">MAINNNDQFATLAVHAGSHVDSHGSVIEPISLSTTFAQSAPAKPLGEYEYSRSSNPNRKNFEDAIAALEKGKYGLAFASGSATTAIILQSLPQGSHAISIGDVYGGTHRYFTKVANTHGVETDFTNNLLEDLPKLVKPQTKLVWIETPTNPTLQVTDIKLVSETIKSINKDILLVVDNTFLSPYLSNPLTLGADIVVHSATKYINGHSDVVMGVLALNDKVLFEKLQFLQNAVGAIPSPFDSWLAHRGLKTLHLRVKQASESALKIATFLENTTDKVIAVNYPGLKSHPQNAVVQKQHRNGLGGGMISFRIKGGAEAAAKFSSSTRLFTLAESLGGIESLLEVPAVMTHGGIPKESREASGVYDDLVRLSVGIEDVEDLLEDIKQALNAASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF23</accession>
    <name>A0A1E5RF23_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Respiratory supercomplex factor 2, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1723</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF23"/>
    <dbReference type="FunCoup" id="A0A1E5RF23">
        <property type="interactions" value="74"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF23"/>
    <dbReference type="InParanoid" id="A0A1E5RF23"/>
    <dbReference type="OrthoDB" id="1915122at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007667">
        <property type="entry name" value="Hypoxia_induced_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040153">
        <property type="entry name" value="Rcf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28018">
        <property type="entry name" value="RESPIRATORY SUPERCOMPLEX FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28018:SF3">
        <property type="entry name" value="RESPIRATORY SUPERCOMPLEX FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04588">
        <property type="entry name" value="HIG_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51503">
        <property type="entry name" value="HIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="HIG1" evidence="7">
        <location>
            <begin position="87"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="183"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ85502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="222" mass="25621" checksum="72DC2E829C57B47D" modified="2017-01-18" version="1">MKLLTEEEIQAHSYYMYQGALKGALAGFIISGLGFRYARTKFPRFHTYPWAMRTAMFITPPTILTSIAAEEASNKFDKIMYSSEADQKAALQEHQKWNAMSTKDKAVTLLSNNKYQIILATWAGSLWGSWRIINKDKLMTGPQKIVQARMYAQFITVGLLLGAMGLSMYEQKLHPDAKTLERQEKWEKILEKAKEDEENLDQNITTRSNEDRIKSKIYRYDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF66</accession>
    <name>A0A1E5RF66_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cerevisin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1805</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RF66">
        <property type="interactions" value="357"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF66"/>
    <dbReference type="InParanoid" id="A0A1E5RF66"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04077">
        <property type="entry name" value="Peptidases_S8_PCSK9_ProteinaseK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000007">
        <property type="entry name" value="Subtilisin-like serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034193">
        <property type="entry name" value="PCSK9_ProteinaseK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF11">
        <property type="entry name" value="CEREVISIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 6" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184722" evidence="8">
        <location>
            <begin position="21"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="10">
        <location>
            <begin position="181"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="9">
        <location>
            <begin position="318"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="27"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="359"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="656" mass="70774" checksum="9C8033FCCD980872" modified="2017-01-18" version="1">MKTTTVIPISLALLAATANGLVIPNKIEKTNTDNSDNSDVAVAKKGKGCHGMKGSSHSSSSHSSSLKKLSFESATDLFKQMVESSTETVESLADSASETLQKNYHFKQEDIKEFISKLKDQIAKVSSKSENSPKDLATFLIQDLVDSANSKGVEGLEVAPMINKNDFNPNQKDTHPLIPNRYMVVFKKNASLNEIMFHQELINSINLDFVESNSNKGEDYDFAQPNEFGISSVNKNGGIENTFNIAGLLQGYVGYFTKEVVDIIRMIPIVDYIEQDSMVFASDFDTQNGAPWGLARISHREKLNLGSFNKYLFDDDAGKGVTAYVVDTGINVKHEQFEGRARWGATMPVNDEDEDGNGHGTHCAGTIASKDYGVAKNADLVAVKVLRSNGSGTMSDVVRGVEYVANAHTKASKDPKNKNFKGSTANMSLGGGKSPSLDLAVNAAVDAGLHFAVAAGNENQDACNTSPASADKAITVGASTLSDARAYFSNYGSCVDIFAPGLNILSTYIGSXSATAVLSGTSMASPHVAGLLTYFLSLQPDKDSAFYDESLDKILTPKLLKQKIVDFATEGVLEDVDAETPNLLIYNGAGEDLSDFWSPKKNKSLETQENEYLENTVEANKLGSQFSIDSLVESLGDSTDNLFEDVRHALKNLNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFK5</accession>
    <name>A0A1E5RFK5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1989</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFK5"/>
    <dbReference type="FunCoup" id="A0A1E5RFK5">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFK5"/>
    <dbReference type="InParanoid" id="A0A1E5RFK5"/>
    <dbReference type="OrthoDB" id="5424147at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024461">
        <property type="entry name" value="CCDC90-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360:SF12">
        <property type="entry name" value="MOZ PROTEIN REPRESENTS A CHROMATIN-ASSOCIATED ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360">
        <property type="entry name" value="PROTEIN FMP32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07798">
        <property type="entry name" value="CCDC90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="37"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="370"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="389" mass="43727" checksum="D3DB1704A2ACF716" modified="2017-01-18" version="1">MASPMKFAFKIKCVSFSSKIRPHGIARKALHTTSLCPLRANSPTDEPSLKSAGSLDTQNGGGNTKPYSLLSLGSMPLLSTDKSSNISLIGKAKQDFSTKQIAKETKTLNSSNTNFLNNNNDDDGYGVMLNSLALLENAKQTPEDIIRQGNLLPATTAKKQVDTMKLYDELMNTKEFSHLQAKTIVNLLVETLDEQFYSTYNYKYVRNMELDNFSYLYDNLSNDLKFELAQLSNNHLMKRNIQILKLQEDLANITEELNQSVITELQKGSKVDFTNHKIENTLLHKDINMRLKQLDYKINTHLFGRLQSQTEQLRWYTMKNGLLALLALVSMVIVGANFNKFINTEKPPPSVVLKTVEKEEDDADEKNNVLGIIGGDSNNHDNELIPNEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFN5</accession>
    <name>A0A1E5RFN5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Na(+)/H(+) antiporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g2219</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fungal Na(+)/H(+) exchanger family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFN5"/>
    <dbReference type="STRING" id="56408.A0A1E5RFN5"/>
    <dbReference type="InParanoid" id="A0A1E5RFN5"/>
    <dbReference type="OrthoDB" id="5327978at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004712">
        <property type="entry name" value="Na+/H+_antiporter_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31382">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31382:SF4">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="248"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="302"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="330"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="413"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="12">
        <location>
            <begin position="27"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ85656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="471" mass="52927" checksum="7FDA1738D63D83AD" modified="2017-01-18" version="1">MVWSHLDIDQQHINYLCISLTGIIFCLTAKFFKNSFYVGEALFFTASGIILGPICLKWCDPFSWTTNVDKISVELSRIILCIQLFTSGLELPSKYMQYNFKSVLLLLIPVMTAGWLIVGSFIYLLIPGFKYKHGLLVAGCITATDPILCQSIVSGKYSQKYVPERLRHLITAESGVNDGLAFPFVYLSLFLIVLDNHKGEILKEFFAVTILYTCILGTVVGLVLGWGSSKLVELSSRHGLIDEQSVHIYYIFLSLLMAGMGSLLGMDELLISFFGGCAFGWDGFYQKNVSSSASPLTDGIDYLLNILYFAYFGTIIPWQKYNNPDLYLSAWRLILIGVVVVFLRRLPVVFTIFKWIPAIKNLRESLFVGWFGPIGVGAVFTSLLAKDYLEKALEKDFISTNHANDIRLGIDTVWVLCSFIVVVSMIIHGFSVPAIMFSKYFRKQPDNIGTLNRSSCDISILDKEEQTLEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGH6</accession>
    <name>A0A1E5RGH6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcription factor tau subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1576</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGH6"/>
    <dbReference type="FunCoup" id="A0A1E5RGH6">
        <property type="interactions" value="1193"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGH6"/>
    <dbReference type="InParanoid" id="A0A1E5RGH6"/>
    <dbReference type="OrthoDB" id="9991317at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039340">
        <property type="entry name" value="Tfc4/TFIIIC-102/Sfc4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23082:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23082">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIIC TFIIIC , POLYPEPTIDE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="187"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="54"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="306"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="325"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1069" mass="125284" checksum="4E38C8C12BBAEEA1" modified="2017-01-18" version="1">MSGSEDEELYENIDDFRALIEDEQQLENNAGTHYDEFMDENDEEYIDPDDVDLMAEFSDDMEEEPDKEGPVKKTEAQEPEEDQDEQDFMDAIREANNFKVKRKKKSGGYKRVKTVDPELAQLLAEANDAYVRQDIAVAEQMYSEVIKKDAKNFAAFRTLGDIYHLQGRLNDCCNSWFLAAHLNPGDWEFWKIVGSLSNELNHHRQAMYCFSKALSVNPEDSECLYERSMLYKRTGQLGRAMEGFQRLYAKHPFDSELVRELAVLYVDYGKAEKAVEMYLKIYEQNVRKRHFIKSYLEEALESSDEEDVAKKKTNKKPLKNNMAQDEFDSDEDEDYGSIENEPGYLAEDYELNALPKKQRLKYRCIFFDWSSLNILIEVYLKHNGTEPVKAINVVKKCARWIQHRENQIFWNDVTNDSEFDDRRERNANYMSLPPIEHEKKYFLPIDIRVRLGLLRIKSKNVVEAMHHFDFLKAETFEDVMDLYYEVGKYLTEAEKYAEALELLEPLCDIEGYDTLEYNLLLGKCYRALELYDKAAFCYEKIVVFSPENLHNKLTLAEMYYHTKNPERFNVLLTEVVETRKLQQEEKDQKEKNKLLGIDEEGDVDMVQLDTNLTDGNVVHASSGIVNDNSVSAKPLMVNIRPIKTSFKRKRRPEEMENYKKEREKKITSSVVDRFNKLYIYKIGLENGDSNQLSLWIDTASDLVDIFTSVRNLFLKSRSQKFVGIITRARKYHKILDYKIERLAKLSEGENLVDGIPVTDEKVPLDQNELRGLTYDQWFELFMELALIITREQSIDDGLSIIETAEEINVFAGDENRVKMMKFVKFTIISQLEDNTEKVELLRALLNQYQFDRKLMQMFLLSISDGGEESFELYNSRQLQKFFLRHLKAFDSTRFGIHVTGQSAIINVSIVDNPQHLPSPYLSFIYGMLLYSSKGYLAALHYLHSIENRVGSDPIFNLSVGLAYLHRSMQRLTPSRNFQILYGFHYLYQYYQIRSENFTEIEEQEADYNLARSFHFLGLNTPAVRYYYRVLNNYKDERLKRHAAFNLIQIFNQSESAELAEEIMEKYLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH25</accession>
    <name>A0A1E5RH25_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone-lysine N-methyltransferase, H3 lysine-36 specific</fullName>
            <ecNumber evidence="3">2.1.1.359</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">SET domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AWRI3579_g1499</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60324"/>
            <dbReference type="Rhea" id="RHEA-COMP:9785"/>
            <dbReference type="Rhea" id="RHEA-COMP:15536"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.359"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.359" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RH25"/>
    <dbReference type="FunCoup" id="A0A1E5RH25">
        <property type="interactions" value="136"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH25"/>
    <dbReference type="InParanoid" id="A0A1E5RH25"/>
    <dbReference type="OrthoDB" id="422362at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140955">
        <property type="term" value="F:histone H3K36 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19172">
        <property type="entry name" value="SET_SETD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000033">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1740.100">
        <property type="entry name" value="Set2, Rpb1 interacting domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006560">
        <property type="entry name" value="AWS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025788">
        <property type="entry name" value="Set2_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050777">
        <property type="entry name" value="SET2_Histone-Lys_MeTrsfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044437">
        <property type="entry name" value="SETD2/Set2_SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038190">
        <property type="entry name" value="SRI_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22884">
        <property type="entry name" value="SET DOMAIN PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17907">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00570">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PostSET"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51215">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51568">
        <property type="entry name" value="SAM_MT43_SET2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 19" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8 19" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AWS" evidence="18">
        <location>
            <begin position="40"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="16">
        <location>
            <begin position="97"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="17">
        <location>
            <begin position="223"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="437"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="686"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="745"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="797"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="946"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="970"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="447"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="702"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="749"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="801"/>
            <end position="821"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="822"/>
            <end position="844"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="848"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="868"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="893"/>
            <end position="904"/>
        </location>
    </feature>
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        <location>
            <begin position="905"/>
            <end position="917"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="946"/>
            <end position="958"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="980"/>
            <end position="991"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018028"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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            <dbReference type="ARBA" id="ARBA00022691"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00030091"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OEJ86209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1092" mass="123119" checksum="9A31145043AA271D" modified="2017-01-18" version="1">MPFVSYIDLENKTKEACQKFQQIDECTYSREGLGRGRRFEEFMECDCSDDWKDGSNKACAENSDCINRLTQIECVNGRCSQCGRDCENQRFQKKQYSDISIIQTEKKGYGVIAQKQIPAQQFIIEYIGEVITEKEFNQRMIDYDLDTEKRKHFYFMMLQKGEFIDATTKGCIARFCNHSCNPNAYVSKWVVKGKLRMGIFAERDIQRGEEITFDYNVDRYGAKAQKCYCGEPNCIGVLGGKVQTDTTNLLPDVFLSALGSSRSAEKKFRQSFKSNKENKKLEIDEEGINKDFLKTLTMEPMHDKDQVSKVVAALLQTRSKALCMKLIQRVMLTKDETLKLEMVKLHGYSAISNVLDVLRAKERYVKIILKYLLTLPRTFRDTIVSSRIDKLVKELGNRYELGEVLDPQIADSAKKLLEKWSTFEVYKRIQKIQTSKGAAPNPAAVSRRQERKQERLNDTKRYTDAAQTLLPHPGNIFSASPIPDRKQVSSAQLLQQGHFTKTKSVSHETTPRTDAFNAGVLKPDAENSVGIGIAENGYLSTQSKFLNNKQEPLSENMIDPLQASLEQTVATKSATPQIEKNQIFSKSAETSQIQTLPPVKTENTELPFLKDLHAKHPVGLSMERTLLSADGTFQKKNLSLDQLEEKFTTPQFDGNAVSAVSPVLAGPNQNATKLTEKRFQAEPLNMSKHSAQPQALPPTSAHALQSPQPYSMNKGIRHMERFQSSPANNPRSPLHDSFSLANENKRHIQSPSTQSNAPNPQLCINQTASQPSTSPFVHAERKPQFAQLDQRQLHRPYNGMSTSRFGPHSSPSSSNRPLQSRFNNKPSRFSNSYKSSDATPNQFNGRKRFIDHDDRQEKDFSSRKFSRDSPTPSQYVPLSKSNEQSRFFHPKPFLRDNRFRDRSENTSGPMPPTNKSADSGVKSFPAGPNSKAGKGVPAKVTVSAATNQPPASVKTQGSLGALTPPYTVKNCSNSWKDNGSRSSSNGGSSSRMNNKTPVEIAESKWKHFFSGFVPNVIFREFSTQKLPHSVCKKIIREFIHSLTDKEIQQNHFDPPVELTANYKKSIMDTAAIYVKKKNTRIMKYARDAENGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH32</accession>
    <name>A0A1E5RH32_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Factor arrest protein 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1339</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH32">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH32"/>
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    <dbReference type="OrthoDB" id="727118at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013258">
        <property type="entry name" value="Striatin_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08232">
        <property type="entry name" value="Striatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Striatin N-terminal" evidence="2">
        <location>
            <begin position="14"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="543" mass="60386" checksum="0F450CFCB41B5864" modified="2017-01-18" version="1">MNDANYLASTVHYSLPGVMHYLQEQFTKNEQNRILWELEKGXLKTRISLLEAENKTLKKKLHQYENTDSSISKEKDSVHSQSNASGKGKGXVSKDVLDVISQSQADLEDTVKEISYLLRPLDLNNYNSQIINNDPNNLMNDATNTLSLEKNNSVLAPNTQQDIPAKQQLPTFSSLTASGAQDQEQXLEAMHHENGLHGNQREENQKSQSQNVTVTTALHKVDIIANNNSFIAIYDSNKGLLQTFSFSSISLASPPSSEFDIVGVRWMWWLQASNYLLVLHKQGIQIINCLTGQITADFSTDFAKLAVADFKNKYLLFNESGTKHLEVWELDFNGALYDNVTTTKKFKIGFECSFKDCKLGITEKSVLLLTSTTLDIFNFETSSLLTSIPLPESFTSPKTSGLLGPSVLSPNTKSKGLSVSAAQLVLNSDSSSVLIHSHDDHRNMNQFLIYSFEKLSFISEFTTNTNLDVSSIXFDSSVITMSYTNGLIELKDMEGKNINAFYCDFHEHCKIKYVSSSKLLISSGLGVPSDADSSKAKLSLKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH63</accession>
    <name>A0A1E5RH63_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Plasma membrane fusion protein PRM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1598</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Involved in cell fusion during mating by stabilizing the plasma membrane fusion event.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cell membrane</location>
            <topology evidence="2 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the PRM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RH63"/>
    <dbReference type="FunCoup" id="A0A1E5RH63">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH63"/>
    <dbReference type="InParanoid" id="A0A1E5RH63"/>
    <dbReference type="OrthoDB" id="3972682at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032220">
        <property type="term" value="P:plasma membrane fusion involved in cytogamy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026777">
        <property type="entry name" value="PRM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31030">
        <property type="entry name" value="PLASMA MEMBRANE FUSION PROTEIN PRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31030:SF1">
        <property type="entry name" value="PLASMA MEMBRANE FUSION PROTEIN PRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0184">Conjugation</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="320"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="389"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="430"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="643"/>
            <end position="664"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ86248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="670" mass="76380" checksum="B4806B1C1526C107" modified="2017-01-18" version="1">MPELKPYLSFQDRLSQIWINKYTILCMLFIFKLLVFSSQVQKSLNNVKSSITESNSACYYYDLMVNETPYYMSLMANYMLEKSIEESIKMSLKILSEVVYIVEQIIIFMFDMWCGTYLCLIMSAVDGAVDVATNVTESLVDLANSTMHSAAQDINKGLSGLSSLVNGVVTEFDKLENELGSLFNKNTDKENSSTTQMSTKISNISLTVSKLSNFYINSDIDSKLRKLSKETPDFDTVKNKTHSLIAIPFNKVIAEFKQVNTTRYVKPANISLKPVQFVKQLNQTGNKNTKNTLTTSYDYCENVILPEFTSFFKKFNKIVLVFQIIVVILLIVVALASCVPEFYKEFFFWKKIYKFKDRVDNNLSEEEVGGLENPFDIEKPARKTFEMDVIGEMFTVFPGFVGVSVLNMLPCFDSQNVKTRWWIHYVFSKRAAILFMISISGLICCLIQYILVRYMEKLTNQIFPKTTPSLASDKQATIITNVMGNMSTMMNSSFQSWSTNTNAFIDTVQYNINNEMVIRSIETITDSLNTTATSVLSDLNGVINKFFNNTILEKPTMSIMHCVIGNKLESLIQEFTWVHDEFQISIPKPSTSELGELYNSVTSNKDSNASNTFETHMTSIFQKIGHDLSEIIVFYKATIKNECILWCTLLALWLLQVPLGLIILTCRYKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHA6</accession>
    <name>A0A1E5RHA6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SVP1-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1104</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat PROPPIN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHA6">
        <property type="interactions" value="392"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHA6"/>
    <dbReference type="InParanoid" id="A0A1E5RHA6"/>
    <dbReference type="OrthoDB" id="1667587at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048720">
        <property type="entry name" value="PROPPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11227">
        <property type="entry name" value="WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="230"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ86280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="323" mass="37049" checksum="9EDAAE0365926092" modified="2017-01-18" version="1">MLCDPRENSQQESLSKTVLSPKYTFNVWDDAAGVVVYEAHDILNFFVSNKKNQFLLLTADLKVRNFKIRSDRIKEFGMPEIICMNEHLDFKKQLLALPSAKKVGQVQLFKVPLDNNDSHTTMEMGVFKAHKTSILNLKTSNNANYIATCSIKGTLLRIFLISYSKSTISANLLCEFKRGLEPATVYEMQWSPDDSRIACISNKNTLHIFQLPDANIQGLSNLEESQMIPEPKKLGEQKTQSDGRRKSVGETQEKKKTXKETLRTSICSIRPKVTVIGDRCKINWHDRDTLYVXWIQAGVWEKYIIQKTDKGYSITKSDWKFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHB8</accession>
    <name>A0A1E5RHB8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor TFIID subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1588</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHB8">
        <property type="interactions" value="671"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHB8"/>
    <dbReference type="InParanoid" id="A0A1E5RHB8"/>
    <dbReference type="OrthoDB" id="308861at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09839">
        <property type="entry name" value="M1_like_TAF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057345">
        <property type="entry name" value="Ig-like_TAF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037813">
        <property type="entry name" value="TAF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057991">
        <property type="entry name" value="TPR_TAF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25316">
        <property type="entry name" value="TAF2_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25577">
        <property type="entry name" value="TPR_TAF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 2 Ig-like" evidence="8">
        <location>
            <begin position="735"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 2 TPR repeats" evidence="9">
        <location>
            <begin position="964"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="354"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="854"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="9"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="354"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1596" mass="183315" checksum="DF79161FE1491A94" modified="2017-01-18" version="1">MDTKIMPMSNGNQRFSFTLGSEPTNNTTGANKITNVQQPQQPNLQNGKHTLGPNASPNQSKPIPFRLNFNTPMIDSMSDFAQNNKPLLESAAIPDKTATRNNTFKIGEQKVQLDVDMLKNGITGITEIVVFPQDNKLTEVIFDCQDTINVIDVSIFWFTGTQTLMYSDLNFNHYNSKTFLEDTINVTNTTLAGNLTNEQIDPFFTSGNNVHQINEVKKWKSSSKFDPINGKRPTSKLCIPVPLEIIQYQQFQPSDALSPFTVKVEYYIENPKEGIVFNTSQFHDLRLRSCYTNNNLVNNSTSYWVPCLESLNEKYLWQLEIMTPRTIKDIYYLNQDDLTTAYKDQLANYRNIIDDEDSDNDGEVEDNQHSAPSKHYRSRNFDIDVICGDATTEKIQIVNKHSKKTVFQLLNPVAPQHVGFVIGCFGKVVLPSISSSEMSTSNTASRSFSTADDLSMQSIPLTVYYPLLKSTKRNGIEPSIQDTEEDSTCDYTQSDQDKYEEDIINTVMTLPKMLDFYIKNFGSFPFNSYTICFLPNLIVPTLNFQGLSLLQSAMVYSSSELDPLISTTIELSKTFATQWAGINLSPKTYDDLWCCFGIAGFMSLRFIRQLMGNNELKYRLKMWNEQIVEEDYNKPPLGLVSPFLSYPLSLDETHSNDIIEFLKLKAPMVMYILDRRMTKMERSFGIIRVVQKLLVQALSGELVNGALSTTTFQHMCERVYKDKLENFFQQWVFGSGVPILRITQKFNKKRMMIEMGIKQVHIEESTNPHLKKKNDITENAWNFVHDASVSLNAAERAASGNLKDLRTPFFTGSMTIRVHEADGIPYEHVVRLTEEYSRLEVQYNTKYKRLRNRRRVNEKKISEQHQTGNDGVSEGDGLLDDANQNSQRAMDMIEDGVTDAEILRMNCLGDVLTSKQDCRDWNLTDFVVTSDNLNENQLQNEAFEWLRVDTDFEWICKIHINQPDYMFASQLQQDKDVEAQVDSIKFFEECIEHSELNSAVYSSILTRTAVDERYFYGVRLEAIKALGKFNIKVEDYQTFHGGIFHLLQMYKTWYCYWDNPSMPLNNNFSDLPHYLVQETIIAQFSTLQDETGEILPKVKNFVLQMLKYNNNENNEYDDTGHIINLINCLMKFVISNKRDKEWNLKAWEVVERFERLDKWKPTSLFKICTTVTKWKVRLFIAEQHQPFFNGEDFLQRLFENCWLENLRTKYPSVPTHDLIQFFLFNLKCVTIFGIKNKEFLKFFFEAVCFNLEPFISHGGINVLVETINFIAVNGFKDTYMNDDVESFQKKYNPHYRSDISKYQHLEDFSDDDDESDEMIDGLPSTKRIDQTEIFFGSSTKDDNSIAADQAISKNSVLARKEAKLKETVEGLIKLLRQTFECYEPLKQILWFLMNSPLLDDYQRKKLFDLIPVIFEQHRRYYVALARPKDRKLVAVSTGDCCVKIKKNDRSKFDPPPQPSIVPSAPLHSKPLKIKLKSVAARGEKRPNTSGKTSAKSKPMVAALPKIVVPKPVTPSAKKSVAAQNLTKKVVKPVRPVKSLVTKVGGLPLAHVKISTKKKKVAVSSLPFNPNLQLLKATTRSLKFRLVYEKSKMQPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHI2</accession>
    <name>A0A1E5RHI2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">CTD kinase subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1479</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHI2">
        <property type="interactions" value="793"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHI2"/>
    <dbReference type="InParanoid" id="A0A1E5RHI2"/>
    <dbReference type="OrthoDB" id="204883at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008024">
        <property type="term" value="C:cyclin/CDK positive transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF546">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="222"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="45"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="113"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="580" mass="66311" checksum="3FCC9C5317AD918E" modified="2017-01-18" version="1">MSSYRGNSNGRRAPSGAGIKNRRGGPGPGNRSRNYHNNIPPRGATSGSFYTQGEPKSTPSRFQDVRVSDQHATLPTPREQLRQQTLPPAVPRSRFNRDSKPRDXNAQTGPAAREXKDTYPKDEYRHKSSVYKGNNKFDXSSRSRFEGHQSNVNMGNNYGGKPNGRGGARFTNDNRPYKKPRFETSXNPTEHTKNTKNELLHXKVLPPKXVKKFAIVSDNLSFKKLKQCGEGTYGKVFKVENLNRLDKKTSLVAIKRLRLETERDGFPITSIREIKLLQRMSTKKSNDNISILNEMLILKEESLDPNIPTKNSINMVFEYCQMDLFSFLEKSSTDLTYGHIKNIGKQLLMGMDFLHSNNIIHRDIKPSNILINGQGAIKITDFGLARIVSTQHEISESSAVHGSLTNRVITLWYRPIELLIGSTNYSYEVDMWGCGCLLLQLINKKSPFQGQSDIQTLYDIIQKLGMPDLSNLPKLFEYPWTFLMLSKLNDLNTYSANQNTKDKIFDKLIESKENSLLEVIKGLLLYNPLERFDAKTCLTYSCFKEVSQLQELHITEDQEYHEYEVKKERREQRKAASRMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHR3</accession>
    <name>A0A1E5RHR3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UPF0592 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1257</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHR3">
        <property type="interactions" value="118"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHR3"/>
    <dbReference type="OrthoDB" id="3972662at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013887">
        <property type="entry name" value="UPF0592"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08578">
        <property type="entry name" value="DUF1765"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="705"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="751"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="705"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="724"/>
            <end position="733"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1059" mass="121382" checksum="7DBCDFA295DF74D6" modified="2017-01-18" version="1">MDPNNITVQLYLSLNKFNVLINQYSNAVTSKNKEHTEFNKRISNFLRIHFLSTLRDFTRNILDLDFVRKNDAQKLVFQXSTYIDCFXHWWLSLLNLLTNNNKTQSINTHLSSELISVLLECISRIMTLSSSIVFHLEQKDLSNMTESKIAFQHRLLLTIHFTTNNLRLIKKYRKTFFANNEKSSKKDGMSKYNSKYSAIIPSGQPSNIQYPPRALPHQHPKIALSTESLSQISQSNSSLSSAPSSFKTINGSRWKWYEKLEKYLHQWVGKLIAYSLYHLSVSKTDFYVENKPQNTTNKDFSFDLLFFNQLFPYSVIKTENHEYEFIANKDVXNDPSSGQQEASKDYNKHRLAHLKTVIEEALPALKILLSYLGNTPYFMSFYYHYLFINFYHLNLFDCSKFFGFHLLQRYIGSKLDADFVAIMKRNGGNNSTNAENTMNAVATSAAGSNSNSTQDKVFETDELFFRKTFLFKLYDTILELPCNEFSQCNLLFKYHECVVLEHIKRIPAHNITLSNYIYQHFLLHKLNNLQSCCPSKLRIIDWKAWLSGICNMWQTLNCEVQIIAVCSLFNIWCFLPIEHQNNLIDEICQHYWDLLFTNQKFHCVTITFCKFLVFQNISLEFDQIAVKLKELQEXNLLYYNANPDFFAKSLVSFNQTNDFLLIPGFKKFILFPSANNKFLQKSEQYPFDVFDELLEKLKPXSTEMSVTMNSKNNGNDKMLPPTPDANSXADKNNASNSLGTMLSFLSFTKAANSSSSTQPPQVPDKNGLAVSTKKRLPXPPELKLYREHYQASLENPISIFQNVSIFTKSSAISAVKTPIQMVMHANQKWGVNSNLCNSASQTLLPSSRNNTSQKHFDKKPLPAINKTANTSQKIAPKLSSPPPPLPPKAYFHSNNASGMSTDSYMEFTQFMKSDTLLDNFKFDTLSSLPSFESSTGLANGHSTDXMHASKEHAINATEGAALGMXQIQKNELFLHQDFFGQESDQHNVSSVTSEETCSSKXFQRDLLLRTKIXQTRKLVATLNQTLEEYRSILPAERSSPTFILYNTPQRKNVVTLPTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHS5</accession>
    <name>A0A1E5RHS5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Dihydroorotase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1222</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHS5"/>
    <dbReference type="FunCoup" id="A0A1E5RHS5">
        <property type="interactions" value="263"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHS5"/>
    <dbReference type="InParanoid" id="A0A1E5RHS5"/>
    <dbReference type="OrthoDB" id="1670005at2759"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00117"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004151">
        <property type="term" value="F:dihydroorotase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00219">
        <property type="entry name" value="PyrC_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004721">
        <property type="entry name" value="DHOdimr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00856">
        <property type="entry name" value="pyrC_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43137">
        <property type="entry name" value="DIHYDROOROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43137:SF1">
        <property type="entry name" value="DIHYDROOROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001237">
        <property type="entry name" value="DHOdimr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00482">
        <property type="entry name" value="DIHYDROOROTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00483">
        <property type="entry name" value="DIHYDROOROTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ86133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="373" mass="41263" checksum="2ADD3AB3404851D3" modified="2017-01-18" version="1">MNIRLPLNPQSTTAYSNNMSFSGSTTEFDLGITCDMHVHVRDGAMCDLITPTIKDGGVSVAYIMPNLQPPITTIERVVAYKAKLEKLAPETTFLMSFYLHESLTPELVAEAADLGAIQGIKCYPAGVTTNSNQGVDPNDFSKFYPIFKVMEKKNLVLNLHGEKPPVADGDITVLNAEAEFLPALKKLHQDLPNLKIVLEHCTTADACEMIQEINSDNVVASITAHHLSLIVDDWCGNPINFCKPVAKLPKDKRKLVEVALSGDKHFFFGSDSAPHPIENKGKCCGCAAGVFTSKYCIPYVAQVFQDNQGDLINFKKFVSENALTFYKPANIKSKKHCVLYKNEVQITNEISQGDIKVVPFKFGDKLDWDTKWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHT3</accession>
    <name>A0A1E5RHT3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">U3 small nucleolar RNA-associated protein 11</fullName>
            <shortName evidence="6">U3 snoRNA-associated protein 11</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1447</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the ribosomal small subunit (SSU) processome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Nucleus</location>
            <location evidence="2 6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the UTP11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHT3"/>
    <dbReference type="FunCoup" id="A0A1E5RHT3">
        <property type="interactions" value="738"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHT3"/>
    <dbReference type="InParanoid" id="A0A1E5RHT3"/>
    <dbReference type="OrthoDB" id="29058at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007144">
        <property type="entry name" value="SSU_processome_Utp11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12838">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12838:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03998">
        <property type="entry name" value="Utp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015952">
        <property type="entry name" value="U3snoRNP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="260" mass="31249" checksum="C0FFDD22A694345B" modified="2017-01-18" version="1">MAKLVHNVQKKQHRERGQTNERSKYGFLEKHKDYVKRAQNFHKKEKTLKILREKAQERNPDEFYYGMHTKRMDPRTGLLVKDRGGSEDDGVLTMDQVKLLKSQDVNYVKTLRQENIMKINKLKNELGFKSKGDHKIFVDDMQEMNDFTPEKYFNTTTEMLERKENRLRLDQMATFGDEDFNADTKKNVQPVETIMPKQSLDKKKLKKYKTMQQYLQREKQLTEVYEKMVQKNELMKNGSKKKVQVDDEKVIFKWKKQRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHZ6</accession>
    <name>A0A1E5RHZ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Transcription initiation factor IIA subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1336</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TFIIA subunit 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHZ6"/>
    <dbReference type="FunCoup" id="A0A1E5RHZ6">
        <property type="interactions" value="501"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHZ6"/>
    <dbReference type="InParanoid" id="A0A1E5RHZ6"/>
    <dbReference type="OrthoDB" id="586585at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10014">
        <property type="entry name" value="TFIIA_gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10145">
        <property type="entry name" value="TFIIA_gamma_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.190:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIA subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.18.10">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.190">
        <property type="entry name" value="Transcription factor IIA gamma subunit, alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009083">
        <property type="entry name" value="TFIIA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009088">
        <property type="entry name" value="TFIIA_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003194">
        <property type="entry name" value="TFIIA_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015871">
        <property type="entry name" value="TFIIA_gsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015872">
        <property type="entry name" value="TFIIA_gsu_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10966">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02751">
        <property type="entry name" value="TFIIA_gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02268">
        <property type="entry name" value="TFIIA_gamma_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009415">
        <property type="entry name" value="Hum_TFIIA_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47396">
        <property type="entry name" value="Transcription factor IIA (TFIIA), alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50784">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor IIA gamma subunit N-terminal" evidence="10">
        <location>
            <begin position="7"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription initiation factor IIA gamma subunit C-terminal" evidence="11">
        <location>
            <begin position="63"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR009415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ86213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="138" mass="15515" checksum="39B4F78DDE862FAA" modified="2017-01-18" version="1">MSTSTHYELYRRSTVGSALVDALDNLISEGRIEAGLAMRCLEVFDKVVSETLKDKTASKMTIKGHLHTYRFCDDVWTFIIKNCELKLEHITDLQSGSKDDSQPAKETKGKNSDINTEIKTLECEKLRIVACNARKSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI49</accession>
    <name>A0A1E5RI49_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Polyamine oxidase FMS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1324</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RI49">
        <property type="interactions" value="360"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI49"/>
    <dbReference type="InParanoid" id="A0A1E5RI49"/>
    <dbReference type="OrthoDB" id="5046242at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF410">
        <property type="entry name" value="LYSINE-SPECIFIC HISTONE DEMETHYLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="1">
        <location>
            <begin position="13"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="492" mass="55844" checksum="01C38C29F3290CE5" modified="2017-01-18" version="1">MIEKKVVIVGAGISGLRCAQKLHESGKFEKDEIIILEARGRCGGRIFTVDGVKSRYDLGASWHHDTLSNPLFKEEAALGKSKFVFDDDFTMMVDAHGDVKSFDSDLKLEILKEECNKFIENEFYSSLEYQDVSYFEMINRYIFDRKEFLSDKQILNLYKSIRNLELWHGIDWKNMSGKYAQVDNLGRNALCMQYDSVINRIKDTIPSSNIKLNNPIEKIERRGNKVIIDDEYQCDYCVVSVPQSVLNLSLLDGIKTKGKIAFIPPLNKDIQECFQKINYGALGKVIFEFDNXWLCKNKKLYYMGTLANNEKSIINLIRNNNALSTDVAAKLNTLVPPYQDKQQAFDLMQFPLLICNYMGISGTPALMILTQSPLTPHIEETYQKHGSLAINEMFAPVLDLLFTKLNPQGPEKSSPKIINTIVSDWTLDPYSRGSYTACFPGDDPLDLSIMMERGQDSRIRFAGEHTINDGTGATYGACMSGEREAAYIAERY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI83</accession>
    <name>A0A1E5RI83_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Translationally-controlled tumor protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1340</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in protein synthesis. Involved in microtubule stabilization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TCTP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI83"/>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Translationally-controlled tumor protein homolog"/>
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        <property type="entry name" value="Metal Binding Protein, Guanine Nucleotide Exchange Factor, Chain A"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Mss4-like_sf"/>
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        <property type="entry name" value="TCTP"/>
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    <dbReference type="InterPro" id="IPR018103">
        <property type="entry name" value="Translation_control_tumour_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018105">
        <property type="entry name" value="Translational_control_tumour_p"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11991">
        <property type="entry name" value="TRANSLATIONALLY CONTROLLED TUMOR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11991:SF0">
        <property type="entry name" value="TRANSLATIONALLY-CONTROLLED TUMOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00838">
        <property type="entry name" value="TCTP"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR01653">
        <property type="entry name" value="TCTPROTEIN"/>
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        <property type="entry name" value="Mss4-like"/>
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        <property type="entry name" value="TCTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51797">
        <property type="entry name" value="TCTP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TCTP" evidence="7">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="167" mass="18630" checksum="59C23773D8D786B1" modified="2017-01-18" version="1">MLIYKDRISGDELLSDAYDVTLVDGVIYEADCTMVKVGGGDIDIGANPSAEGGDEDAEDGSEIVNNVVYSFRLQQTAFDKKSFLTYIKGYMKTIKAKLQETEPDQIPIFEKGAQAYVKKVIGSFKDWEFFTGESMDPDGMLVLLNYREDGTTPFVAIWKHGVEEEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIB4</accession>
    <name>A0A1E5RIB4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RNA polymerase II degradation factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g1342</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Recruits the ubiquitination machinery to RNA polymerase II for polyubiquitination, removal and degradation, when the transcription-coupled repair (TCR) factor RAD26 fails to efficiently displace stalled RNA polymerase II. Also involved in telomere length regulation. Binds DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DEF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CUE"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0779">Telomere</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="CUE" evidence="17">
        <location>
            <begin position="40"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="108"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="277"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="595"/>
            <end position="622"/>
        </location>
    </feature>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="813"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="13"/>
            <end position="35"/>
        </location>
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        <location>
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            <end position="151"/>
        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="196"/>
            <end position="224"/>
        </location>
    </feature>
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        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="287"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="347"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="375"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="410"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="443"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="457"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="482"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="645"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="823"/>
            <end position="835"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005491"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00020536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ86616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="866" mass="91480" checksum="E23C8AAC3C59DF5F" modified="2017-01-18" version="1">MTSIRNSDKKKNTTNSTNNNNNNNHNTNNNNHNNSKLSSEITFKLNTLKELFPDWTHDDLIDLVTEYEDLETIIDKITSGEVTKWDEVTKPVKTKNKKHDNDVFEFNDSVPGHSHVTAGGNINNNNNLNNTNNNNNNNNNNNTNNNNNNSNSGVGAGHSHSSTINGHGHHRYGNNSNSNSSGNQQHGKSRNPHNVPSSALSSVSSSSTSPTSSSAAGASSNPTTNPSLNGNKSLIGSNTPSTPVITPSHAPAGSKTSSKIEPGVSWADKLLSVKKNQEKEAARKAAAKNAKLAAKTNKPIANTTTTTTTTTATTNSATTPSSTSPSSSSSSSSSNAKPGKQILEDVSALKKEIENVEQKQQKNESPQSAKLQAVKQEKSASQESNKDNGSAQQAKADIKEEAPIEVQELSADDTEDFAEQVENAPAPTKKDLKTESAPVAETGTLSAQDASTAVPKQQEELKEQHQEPSKPKEQEHPEAATQAAGASSVQEQQQAAAQAAQQQAAAAAAAAQQQAASQYQQQYANYQQYQQPLAGQYGAQSSQPQQYDYVQAANASTAAGAFSPHQQAAQYYMYQNYPGYSHPAMYDQSYMGMQQPQQGYGQQQQQQQQQQQQQYGSAMGGAASQYPGYSSGTAAASAGSSAADLAQQGSAASPSPSNVQLQQQQQQQQQQQQQPYGSSFMPYGYFYGQSFPYGQPQYNTAAAAAASMNQYGQQYQAGKYNYGYGAPQQQYPPQSTQPNQSGAAGASASPQQDAQGTNAASSSGVAGDDVAGAAGASSASGAAIDPQQQQQQQQQAQFQQYYQYQAQQAMRNGGAAGSGAAPVNSSASATPQQAHSQLGYGASNAGANAYNQYAGYGYSSGTGGYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RJ61</accession>
    <name>A0A1E5RJ61_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RJ61"/>
    <dbReference type="InParanoid" id="A0A1E5RJ61"/>
    <dbReference type="OrthoDB" id="10598635at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="117"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="128" mass="13403" checksum="7B44EC06AA4969B0" modified="2017-01-18" version="1">MFKSGTKFRLNGFLIPALNFKNKMALRNYMYSKHKRTDPTIALIAGSHGIDSATVGAPGKSSSNSNVSSSGNSNNNASSSSSSSNNSSDSIHSGKEQSAEAGALFAGSTKNIDDSDNLNSNSRSNSYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN45</accession>
    <name>A0A1E5RN45_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ferroxidase</fullName>
            <ecNumber evidence="3">1.16.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g877</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>4 Fe(2+) + O2 + 4 H(+) = 4 Fe(3+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.16.3.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the frataxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.16.3.1" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN45"/>
    <dbReference type="FunCoup" id="A0A1E5RN45">
        <property type="interactions" value="379"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN45"/>
    <dbReference type="InParanoid" id="A0A1E5RN45"/>
    <dbReference type="OrthoDB" id="1897642at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034986">
        <property type="term" value="F:iron chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.920.10">
        <property type="entry name" value="Frataxin/CyaY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017789">
        <property type="entry name" value="Frataxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002908">
        <property type="entry name" value="Frataxin/CyaY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036524">
        <property type="entry name" value="Frataxin/CyaY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020895">
        <property type="entry name" value="Frataxin_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03421">
        <property type="entry name" value="FeS_CyaY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03422">
        <property type="entry name" value="mito_frataxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16821">
        <property type="entry name" value="FRATAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16821:SF2">
        <property type="entry name" value="FRATAXIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01491">
        <property type="entry name" value="Frataxin_Cyay"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00904">
        <property type="entry name" value="FRATAXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01219">
        <property type="entry name" value="Frataxin_Cyay"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55387">
        <property type="entry name" value="Frataxin/Nqo15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01344">
        <property type="entry name" value="FRATAXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50810">
        <property type="entry name" value="FRATAXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0409">Iron storage</keyword>
    <keyword evidence="6" id="KW-0410">Iron transport</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ88307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="194" mass="21943" checksum="3B751E216702F8E4" modified="2017-01-18" version="1">MKKSITKTLSKSRPSLLSNLPYLPKWHCALYTLPNILVNKSSVVEAHPNSARRYFQSTSIKKNTNTGGSASTTGLSVPEEILKLTQEQYQKVSDMYLESLLDELEAISEENYDLIPEVELNQGVLNFEIKGKGSYVVNKQPPNKQIWLSSPISGPNRFDFYQNNWISLRDNTNLTKIINKELQEASNGKYSLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN59</accession>
    <name>A0A1E5RN59_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitochondrial glycine transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Solute carrier family 25 member 38 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g881</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mitochondrial glycine transporter that imports glycine into the mitochondrial matrix. Plays an important role in providing glycine for the first enzymatic step in heme biosynthesis, the condensation of glycine with succinyl-CoA to produce 5-aminolevulinate (ALA) in the miochondrial matrix.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>glycine(in) = glycine(out)</text>
            <dbReference type="Rhea" id="RHEA:70715"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Mitochondrion inner membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the mitochondrial carrier (TC 2.A.29) family. SLC25A38 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN59"/>
    <dbReference type="FunCoup" id="A0A1E5RN59">
        <property type="interactions" value="106"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN59"/>
    <dbReference type="InParanoid" id="A0A1E5RN59"/>
    <dbReference type="OrthoDB" id="1924968at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015187">
        <property type="term" value="F:glycine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904983">
        <property type="term" value="P:glycine import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000103">
        <property type="entry name" value="Mitochondrial glycine transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03064">
        <property type="entry name" value="SLC25A38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030847">
        <property type="entry name" value="Hem25/SLC25A38"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46181">
        <property type="entry name" value="MITOCHONDRIAL GLYCINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46181:SF3">
        <property type="entry name" value="MITOCHONDRIAL GLYCINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10 12" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="6"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="109"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="217"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ88320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="304" mass="33651" checksum="7807E4828876E87D" modified="2017-01-18" version="1">MPYTSDKTTPHLIGGFVGGLTSALTLQPFDLLKTRLQQERNSTISKCLNSINHPLELWRGSLPSCLRTSVGSALYLTSLNVVRSKIANSKQAGQGTSIASHGSSNLPNLSAKENLLSGAFTRAFVGYLTMPITVIKVRFESTFYRYKTLKEAVLDIYRNHGMRGFVKGYGPTVARDAPYAGLYVLFYEKNKSFFPKYILPSNFIQTDPKTGKYVTSTATIVNTTSALFSAVLATTITAPFDTIKTRIQLQPNVNKNSIITIKNIIQKESIFNLFDGLSLRLIRKSLSAGIAWGIYEELIKKFLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN70</accession>
    <name>A0A1E5RN70_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g647</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN70"/>
    <dbReference type="InParanoid" id="A0A1E5RN70"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="353"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="392"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="450" mass="50297" checksum="4BA419D0A3332ECB" modified="2017-01-18" version="1">MLLSQSADSDLMFQFNSPIQKNMIQTIRGNSNTNTNNNHTAGSTSVSATNPNTNNVLLMDNAAYNPDYNYNPKNNSYNYRYIAGNNEHIKSRLSQHMNPVMVDNDYIIESKPSHESMDFDDDFELEAGPTSTGDQNNTTSHTINNGINTNHSISDSKISNNSRELVVPSSVTSQMTMEYPPSYKSNNTIDITSLNGDDNSILNNNNNGIVTPDHIAKNIHRLSIDDDENHTKFINADSTGTHHRAINDREYQPLSPPSYSNVFVPPKLSGTVTRNEPAGNASTKHYQKTKYTVGGNNGNKKHYTATMGNISGSRNKLLQKNKESLHKNKLFSHFNNYLNERFVVKDNYSTVANDTSSSSNRHNSTGSTRKSGSNGSVRRGSTAKRSNSRTQSISSGNSNTSRTSSFHDNSEYINNREKWINTQEMVDQYWIYGKDCVLNEDMFESSSDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN72</accession>
    <name>A0A1E5RN72_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative mannosyltransferase KTR7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g868</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN72"/>
    <dbReference type="FunCoup" id="A0A1E5RN72">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN72"/>
    <dbReference type="InParanoid" id="A0A1E5RN72"/>
    <dbReference type="OrthoDB" id="439943at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF2">
        <property type="entry name" value="MANNOSYLTRANSFERASE KTR5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018153">
        <property type="entry name" value="Glyco_trans_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR018153-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="538" mass="63866" checksum="B1885BCD57F47481" modified="2017-01-18" version="1">MVFIKNKYFDYDFKLADIDVSSSVDAPFYYGCNDIKEYLSDPAYQKQNAAFVMLTQNSELEDVLKTMESLELKFNQWFQYPYVFLNDEPFTQEFQDAVQGHTPAKVEFGVIPTDNWDFPEDLESRDTGKRSSTNVQFDSNFEDQEDRGILYGGMKSYHKMCRFYSGFFYKHPLVQQYEWYWRIEPDVKFFCSTTYDPFYEMQKHEKKYGFTIIIDEIYWTIPSLFKHTQAFLKNYKKKVDPTFEVGTLWPMFVDSYKQYEIDADEKFEDSNLLDVFVNHKDQVDEELSRYVEREYYLKNYKPDASSQDTLHTQERVKQHGIGSIIRQGLLKPEIFEDKFDKQEYNLCHFWSNFEIARLDTFDNPIYNAYFQYLDNLGGFYQERWGDAVVHTLGVAMLNKEDIHYFRDMGYQHSTLAHCPKNLANGQVDNAFDETFERKYLPQYKTYRDHTSKFVKNWKTHIQDTISVVFLQNEPSSTTYGSGCQCECPFLKDDIEDASSFCSSKLFEHMSDDYKAGKNPKLTSLLNKVSKGYEQYIHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNB2</accession>
    <name>A0A1E5RNB2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g700</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNB2"/>
    <dbReference type="STRING" id="56408.A0A1E5RNB2"/>
    <dbReference type="InParanoid" id="A0A1E5RNB2"/>
    <dbReference type="OrthoDB" id="364348at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="2">
        <location>
            <begin position="248"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="127"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="297" mass="33593" checksum="E49F10BDF4653BED" modified="2017-01-18" version="1">MSFLDLEKNGTQTHTAEVDKSSGIRIEELNNLVDDLDGCIIDLNRGVKHVLGNKKRDNLGVRQELEKSIQDMNARISEYELTTGFQPMGKNFVNKVERDLSGLIMEFEVLKGQYYKRKLMFPIYTTNETSNGTETSNSSDESLLRDAKNTGSKQVQKQNYGTFSSKDSTTVNKGQEPAQHVETSQWDAGTGGKDATYYSIPNNGASHEDDSQAQMQLQVETEARDQQVQQQLQQAQDHMDQQLLDEHELVVGEQETQMNVIHDQVTEVNLIFKQLGHLIQEQGTQVDKTDADLCFWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNE0</accession>
    <name>A0A1E5RNE0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">2-(3-amino-3-carboxypropyl)histidine synthase subunit 1</fullName>
            <ecNumber evidence="3 11">2.5.1.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g840</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-histidyl-[translation elongation factor 2] + S-adenosyl-L-methionine = 2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36783"/>
            <dbReference type="Rhea" id="RHEA-COMP:9748"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="EC" id="2.5.1.108"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="11">Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the DPH1/DPH2 family. DPH1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.108" evidence="3 11"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNE0"/>
    <dbReference type="FunCoup" id="A0A1E5RNE0">
        <property type="interactions" value="640"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNE0"/>
    <dbReference type="InParanoid" id="A0A1E5RNE0"/>
    <dbReference type="OrthoDB" id="1649088at2759"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11840:FF:000001">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11850:FF:000001">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11860:FF:000002">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11850">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042264">
        <property type="entry name" value="DPH1/DPH2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035435">
        <property type="entry name" value="DPH1/DPH2_euk_archaea"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762:SF1">
        <property type="entry name" value="2-(3-AMINO-3-CARBOXYPROPYL)HISTIDINE SYNTHASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004967">
        <property type="entry name" value="DPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01121">
        <property type="entry name" value="Diphthamide_biosynthesis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR004967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ88402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="426" mass="48778" checksum="3B0C8415BF47C6A3" modified="2017-01-18" version="1">MSEAPKRRRFVGVRKTQGSLEGEEITSSGNVIPSQNTHSLVAKPRNKIRVRNPINHIPDDILQDEELNEAIRLLPSNYNFEIHKTVWNIRKHNAKRVALQMPEGLLIYSLVISDILEQFCGVETVVMGDVSYGACCIDDFTARSLDCDFIVHYAHSCLVPIDITSIKVLYIFVTINIDETHLLKTLKKNFPKGHRIATFGTIQFNPTIHSIKDKLENDEEHMLYIIPPQIKPLSKGEVLGCTSQRLEKEQIDAMLYIGDGRFHLESAMIHNPEIKAYRYDPYGRTFTLEKYDQKQLVQVRSNAIDTAREGKTFGLILGALGRQGNMATVKNLEVLLSKHHKNVVKIILSEIFPQKLAEFEDIDVFVQVACPRLSIDWGYAFNKPLLTPYECNVLFEQEVGFNEKYYPMDYYEQDGYGRGKLPKHHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNL5</accession>
    <name>A0A1E5RNL5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">U3 small nucleolar ribonucleoprotein protein IMP4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g788</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNL5"/>
    <dbReference type="FunCoup" id="A0A1E5RNL5">
        <property type="interactions" value="936"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNL5"/>
    <dbReference type="InParanoid" id="A0A1E5RNL5"/>
    <dbReference type="OrthoDB" id="10253204at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034457">
        <property type="term" value="C:Mpp10 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10480:FF:000001">
        <property type="entry name" value="IMP4, U3 small nucleolar ribonucleoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10480">
        <property type="entry name" value="Probable brix-domain ribosomal biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044281">
        <property type="entry name" value="IMP4/RPF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22734">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN IMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22734:SF2">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN IMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Brix" evidence="2">
        <location>
            <begin position="87"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00040513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="292" mass="33670" checksum="364DB362AB574826" modified="2017-01-18" version="1">MLRRQARERREYLYRKAQELQESQLQEKRLILKQALASGKPLPKEIAEDDNLQKDFRFDESLATQKPTAEEESVDDEYAKQSGIMDPKIIVTTSRSPSSRLQQFTKEIKMLFPNSIRLNRGNYIVKDLVSACVKSNTSDLVVIHEHRGVPTSLTISHFPYGPTAVFSLHNVVLRHDILNSGNQSLVAPHLIFDNFHTDLGKRVVTILKHLFPCGVKKDNTRVITFSNKNDYISVRQHVYVKANTGEIELAECGPRFEMKLFELRLGTLDNKDADVEWQLRRFVRTSSRKDYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNZ4</accession>
    <name>A0A1E5RNZ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mannosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g596</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycosyltransferase 22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNZ4"/>
    <dbReference type="FunCoup" id="A0A1E5RNZ4">
        <property type="interactions" value="953"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNZ4"/>
    <dbReference type="InParanoid" id="A0A1E5RNZ4"/>
    <dbReference type="OrthoDB" id="497541at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF2">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE ALG9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 10 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="144"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="366"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="389"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="426"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ88588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="627" mass="71974" checksum="A0A18363DB592E10" modified="2017-01-18" version="1">MARQRKDLTKKKGTPKRSTQAAVANKSNTTSNVPQQQQDTGSKEIVIQTIDIEPLVQYILYALLFSRIVVQPLYSLISDCDETFNYWEPLNYMVRGFGKETWEYSPVYSIRSWCFLLPLFNVLKVVSKIAKFVPAPLSNFYKPYHLFYLARIILGAVSFLVERQFFIQLCVSFSADFAKWWLLLQLFNPGYFHASVELLPSSFAMTVMLHATNYYIKYVSEKYQNKTKLDFFVKSVACIVVGAIAGWPFVFVLILPLGLHFVLNNFNYMNFIAKGLIGCAKSLFVIVAYITFVDSIFYGKFSPVFFNIVAYNVFNATEESGPNIFGVEPWYYYLQNLVLNFPLVTLLGLLLSFTVFYKFQFFKQCWPVYMQIVIWIGIFNNQPHKEERFLYPIYGLLTFVAAASFVSTHSKNKSQNAKRLPKKPTLGSIVKVFLVLALAGQALLRISSLVINYTGPLAVYEHLNSLSNSTESESVVNVCVGREWYHYPSSFFLPDNHRLKFVSSGFDGLLPGDFQEDVETWVSGIRIVPEGMNNMNLYDEAKVVNVEQCDYLIDVDLPVYDTGKDAINDSTKGKKLYCSSIIDVEHSKLLGRAFTVPFWAYLPKPILKLTSKFYHVEYLDYCLYERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP19</accession>
    <name>A0A1E5RP19_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g918</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP19"/>
    <dbReference type="InParanoid" id="A0A1E5RP19"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="363"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="443"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="471"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="609" mass="65054" checksum="9EEFEAB043C014C6" modified="2017-01-18" version="1">MPPKGVPLSKTQPKDHQGQAPAPFDSHLSQPPHMHSFSRPFVPYQTEAVFNDPNSAVSTPGVSNRLSNDPQLLEQYQQHPLGQNQFFQSNSESSTPYTAGFPGHSQRQNLTQQNAIPGHQMVYGTGGSANGANIPSQIKVTQPSQFTKDFSAHGSQNGESPSMFSQVMVENNGNSAPGAYNSPSAPHSQQVVYQRKQPASNGAIRPTSTLSAKQRQSLLTQIVKQRQPVHQSSLSNLNSPGNQASGFLEYIPEEPHGNAKEIAYEKNSKNNSYAGSPSFTQGKKQNNVSSGTTYNIYSGTTPSAIEGSHSEMLQTATGGNPVVVGSPTVSVKSSTGKSKKKTERALNANSGAKSRAKEDNVAQQQHYQQRQKALKAQQHYQQLIMQQQAQKQGISSTPGPSDTDGSIVSAITPKATGEARANTDSKTPNVDISRISKQDTAKHTSTPGTLGSTKKSNGNGASKKVVKKQQRSGPTLSKGNAKTKSSIQKSNTASKNIDTSSAENVAGATFSQSSESNHKDVAIGNDENTNGDTGSLKPGSVGFGRHTPGLMEDGHMAAPFLLEDLNFDDLAGTLDKDHGLDQNQQLDQFSKDAADLFHLENFGADFMEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP27</accession>
    <name>A0A1E5RP27_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">malate synthase</fullName>
            <ecNumber evidence="3">2.3.3.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g720</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>glyoxylate + acetyl-CoA + H2O = (S)-malate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18181"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.9"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with PEX9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the malate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.9" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP27"/>
    <dbReference type="FunCoup" id="A0A1E5RP27">
        <property type="interactions" value="537"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP27"/>
    <dbReference type="InParanoid" id="A0A1E5RP27"/>
    <dbReference type="OrthoDB" id="186072at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004474">
        <property type="term" value="F:malate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00727">
        <property type="entry name" value="malate_synt_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1220.12:FF:000001">
        <property type="entry name" value="Malate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.360:FF:000001">
        <property type="entry name" value="Malate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.360">
        <property type="entry name" value="Malate synthase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1220.12">
        <property type="entry name" value="Malate synthase, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044856">
        <property type="entry name" value="Malate_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011076">
        <property type="entry name" value="Malate_synth_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006252">
        <property type="entry name" value="Malate_synthA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001465">
        <property type="entry name" value="Malate_synthase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048355">
        <property type="entry name" value="MS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048356">
        <property type="entry name" value="MS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046363">
        <property type="entry name" value="MS_N_TIM-barrel_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01344">
        <property type="entry name" value="malate_syn_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42902">
        <property type="entry name" value="MALATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42902:SF1">
        <property type="entry name" value="MALATE SYNTHASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20659">
        <property type="entry name" value="MS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20656">
        <property type="entry name" value="MS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01274">
        <property type="entry name" value="MS_TIM-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001363">
        <property type="entry name" value="Malate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51645">
        <property type="entry name" value="Malate synthase G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Malate synthase N-terminal" evidence="12">
        <location>
            <begin position="27"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Malate synthase TIM barrel" evidence="11">
        <location>
            <begin position="179"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Malate synthase C-terminal" evidence="13">
        <location>
            <begin position="429"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001363-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF20656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ88626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="562" mass="64123" checksum="D4D5A795E90E8101" modified="2017-01-18" version="1">MAPINLENVQLYGKIDFDETKYPSHSNVHTVLTKECLKFIVLLHRTFNAKRLELLNNRDLLQKKIDSDPSKLVEIFTSNQIPKIRENSDLWSGPVAFGPGLQNRHVEITGPPVRNMLVNALNTEAADAYMVDFEDSSSPTWENMINGQVNLYDAIRNQIDFNNGKKDYKLNDNFVKLNTVMVRPRGWHMDEKHLKIDNEYISASIFDFGVYFYNNAQKLIELGKGPYFYLPKMEHHLEAKLWNDIFNVSQDYLNIPRGTVRATVLIETLPAAFQLDEIVYQLKTHMAGLNCGRWDYIFSTIKKLRNLPNHILPNRDLVTMGVPFMSNYASQLPTVCHKYGISAIGGMAATIPIKDDPVKNEIAMKKVVDDKQRELSKGFDGSWSATPVLCSVVKSVFDKLGTPNQIHYIPDTANINSQDLLNTSIDGFKITTDGIRLNLDIGLQYMEAWVRGNGCVAIHNLMEDAATAEVSAKMLYQWVRHNVELSDKPGQYVTSELTLQILKEETQRLIKENEHRENKFELVAKYFEPEITGTKFSDFITTMLYDEVITPTEAPFDLSLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP29</accession>
    <name>A0A1E5RP29_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytochrome c oxidase-assembly factor COX23, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g723</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the assembly of cytochrome c oxidase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the COX23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP29"/>
    <dbReference type="FunCoup" id="A0A1E5RP29">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP29"/>
    <dbReference type="InParanoid" id="A0A1E5RP29"/>
    <dbReference type="OrthoDB" id="3970270at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033108">
        <property type="term" value="P:mitochondrial respiratory chain complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1130">
        <property type="entry name" value="CytochromE C oxidase copper chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051040">
        <property type="entry name" value="COX23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46811">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46811:SF1">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ88629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="132" mass="15519" checksum="64252832A239DCF6" modified="2017-01-18" version="1">MPEPTSSIPKTSEQVSTNNESNIDISIKNVSQNDEAPKIEIVETEKSKVDFAKNKDYQYYPDKPDSSLNKMKFVVKGASQFYDPCEESSKMSFKCLEMNDYDRDMCRQYFDAYRECKKQWLSHRRSNGNQWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP32</accession>
    <name>A0A1E5RP32_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphatidic acid phosphohydrolase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g916</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the lipin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP32"/>
    <dbReference type="FunCoup" id="A0A1E5RP32">
        <property type="interactions" value="434"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP32"/>
    <dbReference type="InParanoid" id="A0A1E5RP32"/>
    <dbReference type="OrthoDB" id="4567at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000063">
        <property type="entry name" value="Nuclear elongation and deformation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026058">
        <property type="entry name" value="LIPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007651">
        <property type="entry name" value="Lipin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013209">
        <property type="entry name" value="LNS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031315">
        <property type="entry name" value="LNS2/PITP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057124">
        <property type="entry name" value="Ned1-like_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181">
        <property type="entry name" value="LIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181:SF12">
        <property type="entry name" value="PHOSPHATIDATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04571">
        <property type="entry name" value="Lipin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08235">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24565">
        <property type="entry name" value="Ned1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00775">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LNS2/PITP" evidence="4">
        <location>
            <begin position="395"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="259"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="679"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="704"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="748"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="819"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="904"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="958"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="102"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="262"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="276"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="680"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="748"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="761"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="824"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="976"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="994"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1007"/>
            <end position="1021"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ88640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1021" mass="112030" checksum="9187E2D1181ACA9A" modified="2017-01-18" version="1">MQYFGRALGSVTKSWSSINPSTLSGAVDIIVVEQANGDLSCSPFHVRFGKFQILKPSQKKVEVIVNGQPTSIPMKLSENGEAFFVFETEMEHVPEDLISSPVASAASSPMSSPESKTQLEDLASKQRLEKKFVSTDALGSLEEPDFLDINSSGAFSPSNRVNNSSPDLTAAENDTTHLASPVSFTSPTFTPIKKRFEKIKIPTKIDNQTGDLLLDIEGYKYNQDKAHDSDELVQQILKEEFDDSVLSKMIEKDSHGNIRIINHSTPSSPKSFDGNSLSPSISSPSSVVLEQNDTADLGSPPLRSSVSDNTGTLTTTTSSGEFDTATMGLDPPLEASAQEATPKTEKKYIKTIRLTSDQLKCLSLNMGTNDIKFSIGNGKSFLVAKLFLWKWDTPIVISDIDGTITKSDALGHVMTMIGKDWTHAGVSKLFNDIYNNGYNIMYLTARSAGLADSTRAYLKSIDQEGYKIPDGPVILSPDRTFTALKREVILKKPEVFKMACLKDIRDLYLKNFSFDNDDNFETTPKKTPINKNSGGVSALDEDMPTPFIAGFGNRITDGISYRSVGIPKSRIFTINPDGEVHMELLELSGYKSSYLDMNGLVDHFFPPVVIHDEDDDFCNSAISYDNTNGSDLDITHNQSINADNSQGDFVPGYSIFDTINGLETVRSRSNSLGVANAHHVNVDKKNSHHSGRNDEDLNSLSKRISNTQSSAHKRNSSKSRYGTKMSLFRNKEEKFTDTNFWREPLPDIDDLSDLSDEDGSAASTSQSNVPYQQLNSPGSPRVSSPDKSYTMGSRKSSISHHRAKSVDSGFFSSFLRKEDPTETVAGSETDNANEEAISSTPNPSPFLAIDNSLSPVLGDDSLASIRRSAPTTHLSSNSVSASGISPEVGQKIYLNIGSPLSSPKLDSLNYGDDYSYSGRENGNEPDTPSKMPKPMLLPQSPFDLALTSTDEELKKYRSFEDGDNNGLFNGFNSELTEDKLNKDDQASEKPQEPTGKSQLVHQNTMSDEFDEDEFDDDEFED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV10</accession>
    <name>A0A1E5RV10_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CCA tRNA nucleotidyltransferase, mitochondrial</fullName>
            <ecNumber evidence="6">2.7.7.72</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">CCA-adding enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNA CCA-pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA adenylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA nucleotidyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g416</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Nucleotidyltransferase that catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs, which is necessary for the attachment of amino acids to the 3' terminus of tRNA molecules, using CTP and ATP as substrates. tRNA 3'-terminal CCA addition is required both for tRNA processing and repair. Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs. While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded. The structural flexibility of RNA controls the choice between CCA versus CCACCA addition: following the first CCA addition cycle, nucleotide-binding to the active site triggers a clockwise screw motion, producing torque on the RNA. This ejects stable RNAs, whereas unstable RNAs are refolded while bound to the enzyme and subjected to a second CCA catalytic cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14433"/>
            <dbReference type="Rhea" id="RHEA-COMP:10465"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:74896"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="EC" id="2.7.7.72"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.72" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RV10">
        <property type="interactions" value="139"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV10"/>
    <dbReference type="InParanoid" id="A0A1E5RV10"/>
    <dbReference type="OrthoDB" id="3972813at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052929">
        <property type="term" value="F:ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052927">
        <property type="term" value="F:CC tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:CCA tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001680">
        <property type="term" value="P:tRNA 3'-terminal CCA addition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05398">
        <property type="entry name" value="NT_ClassII-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000019">
        <property type="entry name" value="tRNA nucleotidyltransferase cca2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3090.10">
        <property type="entry name" value="cca-adding enzyme, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002646">
        <property type="entry name" value="PolA_pol_head_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13734:SF5">
        <property type="entry name" value="CCA TRNA NUCLEOTIDYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13734">
        <property type="entry name" value="TRNA-NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01743">
        <property type="entry name" value="PolyA_pol"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81891">
        <property type="entry name" value="Poly A polymerase C-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 12 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly A polymerase head" evidence="13">
        <location>
            <begin position="132"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Poly A polymerase head" evidence="13">
        <location>
            <begin position="204"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00050431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00056517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00066885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00072969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00076038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00077436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00080500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ90770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="649" mass="75560" checksum="F44B322BC3BB9C65" modified="2017-01-18" version="1">MLWRNLVSFNRYNIRALNNCIGPKVIITSTRYLHAQSTITINKDKQSANKLLKDKRLKPNNKKKGKKFTQYIKMSVPLLNNTNXNLPNTINHLHPKLSSEIQLNEKESKLCILIKNYIDSYNNVSNTQPLVARISGGWVRDKILGKQSHDLDIAINLMSGEEFVHNMLIFYSKNPLPECFLNYEKDCNNKKNNGKSKNSPFFHNVNTIKKNPEKSKHLETATTKILGMDIDFVNLRNETYTENSRIPIISHGTPQEDAIRRDATLNALFYNISTNQVEDFTQCGLQDLHDGILRTPLDPLQTFMDDPLRILRLIRFSAVYNFKLHESIEQCFVQHHKDLMNALQKKISKERIGVELMKMLDHDLECCKRGLKICCDFNFLTSIFHHCFNENIMEINKDNENEDLIKIEKIYQYENNMESNGPTKNTLNHVCYTKIINNTEFPWVKDNFSSSSPQQSLKSETDTNQSNKILVFSCLLSVFEKIHIYTTIKNKNVKNLKNSMAEWILRDTLRFGKQYYQPVAMIVENISSLHNFIVENLSQPQQKENFKRSDICEKIIXYEEYLPIALEVEKILFGNETYQLIQQQVDSLKLEQVYQVKPLINGKELVSILNKKPGVWMSAFNKQILHWQYDHPTATREDLIQNLGSFTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV18</accession>
    <name>A0A1E5RV18_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA mismatch repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g414</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Component of the post-replicative DNA mismatch repair system (MMR).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RV18">
        <property type="interactions" value="1000"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV18"/>
    <dbReference type="InParanoid" id="A0A1E5RV18"/>
    <dbReference type="OrthoDB" id="10252754at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032301">
        <property type="term" value="C:MutSalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.10:FF:000002">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000771">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003810">
        <property type="entry name" value="PRK05399.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF148">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="DNA repair protein MutS, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6 7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="9">
        <location>
            <begin position="1076"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="70"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="139"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="311"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR037677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ90772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1267" mass="142832" checksum="095404DB99E881A2" modified="2017-01-18" version="1">MSTPKRQKIQATPSSSQKKMKQSSLLSFFKKKEPTTSKXEEVKVKEESKPLTFSTDGSASKGAFDHAAQLKQSQKTEVSPDSSFLNNSKDASSPIKDSTTSNTTIDLPDIPFNGKSTVDGHASAVSSQKKASKIKKEEXGDDDDDDDDEEDVINAFSSQRKRKKLNYNESSGEDDGDDDFETSFSESTKRKRSKLVLTPKNESDEDEYVPDEQSVKQEDAEEQNYTRTIEKSDSSGEDDDLDILALEKKVATKKMQSASSSLMSRLKKSPVKXKPKSKSKSXKSNKEPNXESSSSPQGTQDSRYNWLTKGPRKDANKNPLDHPDFDPRTIYIPQSAWDSFTEFEKQYWNIKKNLMDCIVFFKKGKFYELYENDALLANHMFDLKLAGNGGRAGMQLAGIPEMSFDYWTNQFLQNGYKVAKVDQAESLLAKGMREKQEKTPKTSAKSIVQRELKYVLTRGTLTDEAMLQSXLPTYCLSIKQQDNSFGISFVDTSTGELYIVQVDETGSNDYSKLDTILSQVRAHEVVMERNNLSSXVLKLVKYNSAPGCIYNYLRKDDEFYDVDKTVHELNTKYTKVPEIIAKDFIEDFEKHVALNAFGGLLSYLQFLKLDKNIIEMDKVTNYDQFXXSSTSNQKYMVLDGITLQNLEIFANTFDGSDQGTFFKLINRATTGMGKRMIRKWVMLPLFQKPEIEDRLDSVDQLIDDFEIRELVENYLAKLSDLERLCARVHSNNLKFKEFNNKVLPGFENILEFIQKIKKFELKGSMKEYVARIPEILLNEKLNEWANAYDRNNILGDXQEYSIKLYPGVDQEYDQSLNNINDCAGKLSNELNSYKSLLKTSKLQFKDSGKELYTIEVPIEACKRVPGSWTQLGANKSFKRYYSPKVATLAQEMAEYKEAHKILETNLTERLYSKFAENLNDVWGPTVELIASIDCLLSLCKTSESIGIPSCRPQFVDSDYSFMQFKSLRHPYFNMGSSSTTEFIPNDVELGLEDNNKITLLTGANAAGKSTVLRMTCIAAIMAQIGCYVPCEYAKLTPIDRIMTRLGANDNIMQGKSTFLVELSETKKILDLATDKSLLVIDELGRGGSSSDGFAIAESVLYHCATHVQNLGFFATHYGEGLYLNFAQHPQIRPMKMAILCDEENKNVTFLYKLVDGLSEGSFGMHVANMCGIPKEIVDNAIVAADELEHTSKVLKMLKNGRTEQGSEGTNVDSSTLVPLGVQTDFVRIIEYGINNLHGCGEGVKVYNEQNKKLAVKNIFKMIEGLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV76</accession>
    <name>A0A1E5RV76_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ras-related protein RSR1</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g522</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RV76">
        <property type="interactions" value="252"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV76"/>
    <dbReference type="InParanoid" id="A0A1E5RV76"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000114">
        <property type="term" value="P:regulation of establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04177">
        <property type="entry name" value="RSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000631">
        <property type="entry name" value="Ras small monomeric GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041841">
        <property type="entry name" value="Rsr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="174"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="223"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="250"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="300"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00072720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ90857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="328" mass="36392" checksum="69171131D25C0E73" modified="2017-01-18" version="1">MRDYKLVVLGAGGVGKSCLTVQFVQGVYIDVYDPTIEDSYTKTMDIDGKLVELEILDTAGVQQFTAMRELYIKSGHGFLLVYSVDDENSLSELLKLRDQVLRIKDTEKVPMVLVGNKADLSDMERKISVEDGITVSEQWGKIPFYETSALLRSNVDEVFVDLVRQIIRNEIEEQKRQHQEQQQEQQQQKQAYPKHQIAQNRGHAHSNSTVGSSAHKKTTQLQTGSNGSTSSFNNKPYSAGRYASQDDLGGSRXPSSRTREXSSVSGAVPAGNAKFKQQELPKQSAPGRKVTEQTSKQRSSSKFTKSAAQTKSSSTLKKNKSKNGCVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVE5</accession>
    <name>A0A1E5RVE5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Aspartic proteinase 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g334</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVE5"/>
    <dbReference type="STRING" id="56408.A0A1E5RVE5"/>
    <dbReference type="InParanoid" id="A0A1E5RVE5"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05474">
        <property type="entry name" value="SAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033876">
        <property type="entry name" value="SAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF65">
        <property type="entry name" value="ASPARTIC-TYPE ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184893" evidence="9">
        <location>
            <begin position="23"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="10">
        <location>
            <begin position="77"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="613"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="617"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ90912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="673" mass="72004" checksum="0CC2D7B0C7157AFD" modified="2017-01-18" version="1">MQGKAFAKTLVASALCFQYAQSAIIDEQNIDIVQQQKGVRFGIERGYKVIDSKSKFSKRDDGSNVLKFVIDNQQSYYSVNISVGTPAQNVTALLDTGSSDLWLLGDFNNVTACQAYLNEIGDVSSFSSSSSSSSSSSSKKDVQNNNVVENDIVYANFHRVLNQYQNKQEQQEQVEKAAAVVSTQTVNLESYIATASLPTGFDWDLTSGFSYATESATGTASGVDSSNSAEASQVLQGCKDSGIYTPEDSSSYKKNDSTAPFLIQYGDLTFASGWWASDSIAINGVEVPLQFAVANVSNSTSVLGISFEELESSVASYSNDDDESSKDLLENFTYPNLPVVLKNEGLIDKISYSLYLNSLEQPYGEILFGAVDSTKYTGQLYTIPLVNIYKSQGLENPVEFDVTLQGSGISTAKGTKKTFSTTKIPALLDSGTTLMYLPTELAEMFADSVDAEWDDDLQSYIMECPSDEDQLANSYAFDLGGFLIYAPLRNFVLTTTDPKTCALGVVPQDQLYAVLGDVFLSSAYVVFDMEDYEVSIAQANYDGASEEANIKAIVSTVPNAIKAPMYSSTWSTTASISTGGNIFTASNATNPWTATDNNGASTISVDASGITKTSSYAGSRSSTKTATKTSNGGSSSSSSSSSSKSKNAAVGITAAFSNEGLFATLFVLFSALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVF9</accession>
    <name>A0A1E5RVF9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nuclear mRNA export protein SAC3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g312</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVF9"/>
    <dbReference type="InParanoid" id="A0A1E5RVF9"/>
    <dbReference type="OrthoDB" id="3972203at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070390">
        <property type="term" value="C:transcription export complex 2"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045107">
        <property type="entry name" value="SAC3/GANP/THP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005062">
        <property type="entry name" value="SAC3/GANP/THP3_conserved"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436">
        <property type="entry name" value="80 KDA MCM3-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436:SF3">
        <property type="entry name" value="GERMINAL-CENTER ASSOCIATED NUCLEAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03399">
        <property type="entry name" value="SAC3_GANP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC3/GANP/THP3 conserved" evidence="2">
        <location>
            <begin position="359"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="834"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="170"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ90927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="882" mass="100317" checksum="2684232E34A545B7" modified="2017-01-18" version="1">MSSAFGGFPSSGFNFFNASQDDKKSKSLRFDKNDNNNGSQQNNNFNRNKGKPFTSNSNNRGPFFENVXXKGXGKNRGSFSTSKEKSPVRTSRFSDRSNTKTSSDPLNKQKQSLTKQNFGNNNHNTGTAHFKNKNYGSFQALDKKGNYMSTKSKFNAENSGSNSVTRDNKTNNNYTSGGKSNTNTTTTTTTTTNNNNNNKTNKNNNKVNSFNTNETKPKIINEQKNKKKKNIIHNDNKLTLNPLPHEKENSLGPLLPNPDQNGFFKHDINQRELPKFMIRERAQLLPTEFKQDQWDKANQLKMLQMEETSSGDTQXLYDMFRKMRVMERKMMENKKLVDKAEESKDLSEAIVFQGTCLDMCPIFERTRRSLENNVSNLEKIDGKISREKAIKAFARPAASAPPSLPSDVRPPHVLMKTLDYIVDNVIGNLPEGEPFLWDRMRSIRQDFTYQNYCGPEAVDCNERICRIHLLILHIMAKSNAEYSKQQEIEQLTKALITLCEIYDDARNAGLNLPNEPEFRAYWLLHNIRDSECDYVIQNLPLEVINNKSVQLALAFRRIVSNSNFKDRGHISTPNGLNLYKSFFKLLSDPKTPFLMASFLENYINEIRFYGFLQMKSSTNKKLKPIPFQFFIETFRFNNFQELIDFCHYYSVDIEGSDHVVIQSLEHTNHLLPEKSPLKQAWLQCVDEKMIQLGDFKNIINVGKSNFNKDYLINKHFGISMSQTSSVEDLDEEKMVENGNEAVGAKLSEPVTKPDFTGLNRDSKTDGALVSHSRGDIAINTGKSLFGKGTLTKSFNEPKPSXQSVFSLGNKTXPVAKFDVSSKDAFFDNISLGLKRDQSTTPTPQARDVNKGFAKPSKDELDNMENVSXNYKDKAPISFXXKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RW26</accession>
    <name>A0A1E5RW26_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Acetyl-CoA hydrolase</fullName>
            <ecNumber evidence="4">3.1.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Acetyl-CoA deacylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g490</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + H2O = acetate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20289"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="3.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyl-CoA hydrolase/transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.1" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RW26"/>
    <dbReference type="FunCoup" id="A0A1E5RW26">
        <property type="interactions" value="222"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RW26"/>
    <dbReference type="InParanoid" id="A0A1E5RW26"/>
    <dbReference type="OrthoDB" id="10250396at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008775">
        <property type="term" value="F:acetate CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003986">
        <property type="term" value="F:acetyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006083">
        <property type="term" value="P:acetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.750.70:FF:000002">
        <property type="entry name" value="Acetyl-CoA hydrolase Ach1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1080.10:FF:000003">
        <property type="entry name" value="Acetyl-coA hydrolase Ach1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1080.20:FF:000001">
        <property type="entry name" value="Acetyl-CoA hydrolase Ach1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.70">
        <property type="entry name" value="4-hydroxybutyrate coenzyme like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.20">
        <property type="entry name" value="Acetyl-CoA hydrolase/transferase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.10">
        <property type="entry name" value="Glutaconate Coenzyme A-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026888">
        <property type="entry name" value="AcetylCoA_hyd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038460">
        <property type="entry name" value="AcetylCoA_hyd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046433">
        <property type="entry name" value="ActCoA_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003702">
        <property type="entry name" value="ActCoA_hydro_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43609">
        <property type="entry name" value="ACETYL-COA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43609:SF1">
        <property type="entry name" value="ACETYL-COA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13336">
        <property type="entry name" value="AcetylCoA_hyd_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02550">
        <property type="entry name" value="AcetylCoA_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-CoA hydrolase/transferase N-terminal" evidence="9">
        <location>
            <begin position="10"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Acetyl-CoA hydrolase/transferase C-terminal" evidence="10">
        <location>
            <begin position="333"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ90948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="525" mass="58751" checksum="4117208358F03F73" modified="2017-01-18" version="1">MSSILKQRIRYKPYLSKICNNPKDLIPLFKNEDRLGWSGFTGVGSPKVVPDVLAQHVEDNNLQGKLRFNLFVGASAGAEESRWATNNMIIKRAPHQVGKPIAARINSGDMQFFDKHLSMFPQDLTYGFYSPKDKILNPREILDYTIIEATAIKEDGSIVPGPSVGGSPEFISCSKNVIVELNTAIPSFEGLHDIDMPELPPYKKPYLYTQVDQKSGLTGIPVDPNKVIAIVESKIKDKVPPNTPSDEKSKKIAGHLVDFFKNEIKYGRLPENLHPLQSGIGNIANAVIEGLVDGDFKHLNVWTEVLQDSFLELFENGSLDYATSTSIRLTEKGFEKFFNNWDDFSQKLCLRSQVVSNNPEIIRRLGLIAMNTPVEFDIYGHANSTNVAGSKMLHGIGGSADFLRNAKLSIMHAPSARPTKTDPTGISTVVPFASHVDQTEHDLDILVTEQGLADLRGLSPRERSREIINQCSHPDYKDMLLDYVAKAEFECNKTKSMHEPHYLKNCFKVYENLREKGTMKVDSWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZ24</accession>
    <name>A0A1E5RZ24_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g249</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000002">
        <property type="protein sequence ID" value="OEJ92134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZ24">
        <property type="interactions" value="156"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZ24"/>
    <dbReference type="InParanoid" id="A0A1E5RZ24"/>
    <dbReference type="OrthoDB" id="300289at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14369">
        <property type="entry name" value="CUE_VPS9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041545">
        <property type="entry name" value="DUF5601"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045046">
        <property type="entry name" value="Vps9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041804">
        <property type="entry name" value="Vps9_CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101:SF25">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN AND VPS9 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101">
        <property type="entry name" value="RAB GDP/GTP EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18151">
        <property type="entry name" value="DUF5601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00546">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00167">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109993">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VPS9" evidence="3">
        <location>
            <begin position="123"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="CUE" evidence="2">
        <location>
            <begin position="443"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="386"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="387"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ92134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="487" mass="55492" checksum="83D67E392621CC4A" modified="2017-01-18" version="1">MSESEDHEGENGYDFKKFISHLKDPMAEPIIKYTKSFIQNFMSSERLYSMKEQVRLISDFELFIFEKFSKYEPFKSMNQEQIKNSKDCMEKLITSKIYIKIFSPLVAANNGLLVLDEDHLLNVQQDKKLNEKIKEFRFVTPDMLELSSGSPKTSNAHEDAASTKPFVLLNRFSIMAAKELSKLNRFKAPRDKMICILNSCKILFAFLKRRDKDSNADDFIPLLIYTLLHSGAPALKFGLDNLASNCSYIENFRRSDFLTGENRYYLSSFQGVIQFILEMNENNLKLTNKEEFEKEYSANKQKVLQEEKEEKEEMEMNKKGQQQQQQQQQQQQKQQQQQQRDQATGANDLSLFSGLFASTGETTNSAGAXNGDILGIFSNFLGNGPVSSNDSASPSKASTTDGSVETPLGAQTLASQGKKSKAKTQPPAKQNYMETIRNMEEKEQQNTLDNMQEMFPEIDVDLVKDICIIKKYHTGECIDTLLELSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZB5</accession>
    <name>A0A1E5RZB5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">L2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">RP2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">YL2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g12</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes). uL4 is associated with the polypeptide exit tunnel. uL4 interacts with its chaperone ACL4 and the nuclear import receptor KAP104.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZB5"/>
    <dbReference type="FunCoup" id="A0A1E5RZB5">
        <property type="interactions" value="1571"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZB5"/>
    <dbReference type="InParanoid" id="A0A1E5RZB5"/>
    <dbReference type="OrthoDB" id="10259785at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1370.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025755">
        <property type="entry name" value="Ribos_uL4_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_uL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_uL4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013000">
        <property type="entry name" value="Ribosomal_uL4_euk/arc_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045240">
        <property type="entry name" value="Ribosomal_uL4_euk/arch"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19431">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14374">
        <property type="entry name" value="Ribos_L4_asso_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="Ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00939">
        <property type="entry name" value="RIBOSOMAL_L1E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 9" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL4 C-terminal" evidence="8">
        <location>
            <begin position="272"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00064557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00077544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00079326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00080732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ92291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="363" mass="39007" checksum="2133D6D35DA9794E" modified="2017-01-18" version="1">MSRPQVTVQALNGESGASVALPAVFSAPIRNDIVHSVFTRINKNKRQAYAVASNAGEQTSAESWGTGRAVARIPRVGGGGTHRSGQAAFGNMCRGGRMFAPTKVWRKWNVKVNHNEKRFATVSAIAASSVASLVLARGHRVEKIPEIPLVVSNDLESVTKTKDAVAALKKIGAHADVVKVLKSKKMRAGKGKYRGRRFTQRKGPLVVYANDNGLVKAFRNLPGVETANVESLGLLQLAPGAHLGRFVIWTEAAFAKLDSIWGSETVASAAKSGYTLPSNLVSTTDITRIINSSEIQAVVRPAGQATQKRSHVLKKNPLKNKQVLLRLNPYAKVFAAEKIGSKKFEQPAKKAAGDVFASTLKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S028</accession>
    <name>A0A1E5S028_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">pyridoxal kinase</fullName>
            <ecNumber evidence="2">2.7.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g196</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pyridoxine kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.35" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5S028"/>
    <dbReference type="FunCoup" id="A0A1E5S028">
        <property type="interactions" value="291"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S028"/>
    <dbReference type="InParanoid" id="A0A1E5S028"/>
    <dbReference type="OrthoDB" id="2104723at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008478">
        <property type="term" value="F:pyridoxal kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009443">
        <property type="term" value="P:pyridoxal 5'-phosphate salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01173">
        <property type="entry name" value="pyridoxal_pyridoxamine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004625">
        <property type="entry name" value="PyrdxlKinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00687">
        <property type="entry name" value="pyridox_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534:SF2">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ92368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="352" mass="40138" checksum="514FDF3E46E1CBA5" modified="2017-01-18" version="1">MPNLLAIQSHVVHGYVGNKAATFPLQCLNWNVDCLNVVQFSNHTGYGFFVGSTISEKDLNNILEQLTKNFDYDALLTGYLPNKDCVRTVLTKYTEYKNQLNVSNPKKKCLWLLDPVMGDEYHLYVHTNVVPEYKKYLQMNNIDIITPNSFELQLLYNTDLGFIEKHKALPTLITMEEIYVMLAGLHRNVNFIVITSLDNVDVGEEGDRANFIYCLASWHQQSDKKYLFKIPKIKSYFTGVGDLFSSILLNSLYNSTIFSSSPTSITSEEREAEIIYCVNYTLTILQKVLETTIQQRLLMSPGITADIIESASMGGDPMVMKNMELKIIESKNVFLSTEEKNVLNFLCKKITH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S0M6</accession>
    <name>A0A1E5S0M6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Calcium/calmodulin-dependent protein kinase II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g66</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5S0M6">
        <property type="interactions" value="668"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S0M6"/>
    <dbReference type="InParanoid" id="A0A1E5S0M6"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="37"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ92513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="449" mass="50677" checksum="A47B8054669097DD" modified="2017-01-18" version="1">MXVAQEEACPKEENILKKFVHKHILKQPESYAKKKNYIFGKTLGAGSFGVVRQARRLSTSEDVAIKILLKDALNETNLQMLYDELQILESCHHENIVAFKDWFESRDKFFIVTQLAIGGELFDRIVQRGSFTESDAIAIVKQILSALTYLHSKNIVHRDLKPENILYLNRSGDNKVVLADFGIAKKLEHPQDLIYKAAGSMGYVAPEVLTKRGHGKPCDIWSLGVITYTLLSGYSPFMAESVDGFMEEICTGPEPVVFHAPYWNTISKEAKFFIKKCCTVDPDHRPTAKELLEEDPWILSLSKKDGQSEVAGKEEHDLLPHIKQSISPKLKLQKAIQVVMLNNKIKSLKEKYGIVDEDHDIDNTEGQSKSAVDGENLADDSQVNSDAQAKSIRLLQNQIRQLSLKEDEASKNLRKDITQAAFANLVEFATLQKDYLMKQDNEETTKQGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0G8</accession>
    <name>A0A1E5R0G8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">26S proteasome regulatory subunit RPN7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4534</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the 19S cap proteasome complex which acts as a regulatory subunit of the 26S proteasome, involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or both ends by the 19S regulatory complex (RC). The RC is composed of at least 18 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively. Component of the lid subcomplex of the 19S RC.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0G8">
        <property type="interactions" value="1100"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0G8"/>
    <dbReference type="InParanoid" id="A0A1E5R0G8"/>
    <dbReference type="OrthoDB" id="1452at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.570:FF:000005">
        <property type="entry name" value="26S proteasome regulatory subunit N7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019585">
        <property type="entry name" value="Rpn7/CSN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045135">
        <property type="entry name" value="Rpn7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049549">
        <property type="entry name" value="RPN7_PSMD6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145">
        <property type="entry name" value="26S PROTESOME SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10602">
        <property type="entry name" value="RPN7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21154">
        <property type="entry name" value="RPN7_PSMD6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 8" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="107"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="PCI" evidence="7">
        <location>
            <begin position="199"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="401"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00093435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00093502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="422" mass="47959" checksum="A86CE968E140CE08" modified="2017-01-18" version="1">MTQENEETYVDEPYVSKVPNYEVSELAFLLQKTKAPESYDKLMQKIQGQDMAPYYMXLKDHDYFQGVAAFDETLYNNMKTINDAKVEELKQKITKAEEEDDGELEKAYSWLKLGEYYAQIGDLENAVETLTKAQEVSVSTGAKIDILLTITRLGFFYDDQHFVKQRLEQVNLLIEKGGDWERRNRYKTYRGVHCLSTRNFKEAAELLCDSLATFTSLELTSYEKIATYASVAGLVSLERLELKNKIIDSPEILSMMANTSPLESVFSLAMSLYTSDYASFFPYLLETRETVILPCKYLNVHADYFMREMRRKVYSQLLESYKTLSLKSMADSFGVSVEFLDNDLSKFIPNKQLSCVIDRVNGIVETNRPDNKNAQFHSLVKQGDGLLTKLQKYGAAVRLTGDNPGLAEPETSAKPEASASAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0Q6</accession>
    <name>A0A1E5R0Q6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cell wall mannoprotein PIR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g4397</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PIR protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0Q6"/>
    <dbReference type="FunCoup" id="A0A1E5R0Q6">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R0Q6"/>
    <dbReference type="OrthoDB" id="5415592at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005199">
        <property type="term" value="F:structural constituent of cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054508">
        <property type="entry name" value="PIR1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051153">
        <property type="entry name" value="Yeast_CWMannoprotein_PIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000420">
        <property type="entry name" value="Yeast_PIR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254:SF1">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00399">
        <property type="entry name" value="PIR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22799">
        <property type="entry name" value="PIR1-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00929">
        <property type="entry name" value="PIR_REPEAT_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50256">
        <property type="entry name" value="PIR_REPEAT_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Cell wall mannoprotein PIR1-like C-terminal" evidence="9">
        <location>
            <begin position="270"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="176"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="207"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="354" mass="35444" checksum="3AAC94F1C7DD86C8" modified="2017-01-18" version="1">MQYTKSTVVTAALAATAMAAYAPSEPWSTLTPTATYTNAITDYTHSFGIAVNPITSGSASASASATVSAAKAKRAQAISQIGDGQVQATTSTTVHSSTSSSVSTAKTTAKAVSQITDGQIQATTATTTPSSTSPTTTKTTAQAVSQIGDGQIQATTLTTVSAGKTTVQVVSQITDGQVQASTATSSTTSSKSTASAASQIEDGQIQASTGSASASASATTSASEESESTASASSTSSSASASASSSDVTDPVKAESCASDGTLSMTLKDGILTDGKGRIGSIVANRQFQFDGPPPQAGAIYAAGWSITPEGNLALGDDDVFYQCLSGNFYNLYDESIGAQCKEIHLEVVELISC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0Q9</accession>
    <name>A0A1E5R0Q9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Chromatin structure-remodeling complex protein RSC58</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g4413</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0Q9"/>
    <dbReference type="FunCoup" id="A0A1E5R0Q9">
        <property type="interactions" value="359"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0Q9"/>
    <dbReference type="InParanoid" id="A0A1E5R0Q9"/>
    <dbReference type="OrthoDB" id="5354116at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ80485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="479" mass="55462" checksum="3A171269DFEA1F15" modified="2017-01-18" version="1">MQESIANHSSSHLLEDLQNIFTLTGDNCGVLQEPFIESFYEPESAKITSSYINFLNTTTEGIDNEEQVEKTLAEHPKTTLSKRFAEGYYFKQEHSYYALYHDITISCFLGVNSYTRGSKVYCLVDKFYKFASELILRSCYKIGSDLHLKQFENSEKDKLQTPYLERIEEQFDKISSSYTVPIESQFHISTQELLFFSSLAIKSSLDKRGDMLPHPDMEVIDVIPLEATNTVRAPKLGAILPSISNVPEPTLPSTRILNNFVHPNWYVLPTTVWVRYLDPEYKSLLPTINDFGTMLDCTQRSIFWFEKTGYLAKYTAKEEQILDTLKAQKEQDTDFKLEDDVVHVDISQRKETQEQISVQHLLSWSPTHYFNDEEYEALCSGVPAVQKLISKYMIKLKKVQESRLRNRILQADVLENRLFEKIKRLLKHVLLHASNISDLDVPHVDFIPVLQANYTGSIPIVRKQIIQQPVKKTKKYRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0X0</accession>
    <name>A0A1E5R0X0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nucleolar complex protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4584</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0X0">
        <property type="interactions" value="665"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0X0"/>
    <dbReference type="InParanoid" id="A0A1E5R0X0"/>
    <dbReference type="OrthoDB" id="10263185at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030692">
        <property type="term" value="C:Noc4p-Nop14p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027193">
        <property type="entry name" value="Noc4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12455">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12455:SF0">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCAAT-binding factor" evidence="2">
        <location>
            <begin position="306"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="537" mass="63010" checksum="EE1612A7339375B2" modified="2017-01-18" version="1">MKDQIDALHKSXLESTKNYNNILKLMKLYEAFPQINKLTDSGDSQSIQTLRYLTVSLFKIFYKLSTKLQLNPSMASNANEKLLFQWLKKLYELNFKKNVLLNYMVSMEYENSLSMDCLDIYMKCIELEATFFASKMGAPYFPNKTLSKLIETLFSGASSSSNTNKQYLFDQLSENYYKKYVDIQYYFQIELQELISAGSIPYXTQTSSYWLALMDHDNHYDNANSDLAIFVPNPPSTMENEIKFKTQFEKNWIFILSNPQTTSYQFKQFLTILHKRIIPHFVTPTKLMDFLTDCYDNVENDLSVQLLSLNGLFELMKNYNLEYPNFYTKLYALFKPELFHLKYRSRFLRLIDIFLRSSHLSSNLIAGFMKKMSRSLLTSSPNAIVSVIPMIYNLLKLHPNCMILIHDPDYINPYFTNSKGEIEQRIFHDAFDINEPNPEFSNAINSSLWELETLMHHYHPNVASLAKIFQQPFRKMSYNMEDFLDWNYKSFLNSELKRSLKILPALDHQNKGXCXFADGNTDVQNLQNDVYMDAITW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1G6</accession>
    <name>A0A1E5R1G6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">MIOREX complex component 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g4031</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1G6">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R1G6"/>
    <dbReference type="OrthoDB" id="4046837at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ80717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="552" mass="63164" checksum="C19020AC9497CA49" modified="2017-01-18" version="1">MILTRLVRYRATRSTIHRTGALPILRFFSSQSQVIQKLLQTEAFKKLEPTKDLATLQPYELTLKVNERLSKSSNXMLSINTRAKIYNSLIENLTKYDYSVSSISMVKLESMNQSPTLNAFMEMVKYNPGRKLPTWEMLKENLGLFDKNGDKSVRDKATLVVLLKLLALDPIEIEEGKTLTDSKDLTRIVFLLNNMSDELVLQIEKSVLDKIYEYMLATSSTILLTPFLNKVLKRSDYNMHVIPNEHLSTDLQKAITNIYKVKKDTNDQKAIHXLIELANKHGKSNGIIAPFVTEEKPAEAIMAEVQRVNKLCLTSSFSVQLEVSETLSLSEAVEEMFAKMDFTKDVQLLKLMMRNIGTVKGDLQRCMELYHTYLRHHEDKMEFLQFEGFLSFAIKAYETNNFKLFEQSLFLLPQRSNDLDLSNKVVRALILLNSKFDIEKSLELFNSNIERFKETVHGREQDGSVDKKDAKGGLSLAGMLTECLMLAHLSQKDRDFAHVIFEGAAREKIINGPTSIKQIKSLLAFYGEILELPEKEFEKAMSEKVLSYLKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1M9</accession>
    <name>A0A1E5R1M9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Maintenance of mitochondrial morphology protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MMM1</name>
        <name evidence="12" type="ORF">AWRI3579_g3877</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the ERMES/MDM complex, which serves as a molecular tether to connect the endoplasmic reticulum (ER) and mitochondria. Components of this complex are involved in the control of mitochondrial shape and protein biogenesis, and function in nonvesicular lipid trafficking between the ER and mitochondria. The MDM12-MMM1 subcomplex functions in the major beta-barrel assembly pathway that is responsible for biogenesis of all outer membrane beta-barrel proteins, and acts in a late step after the SAM complex. The MDM10-MDM12-MMM1 subcomplex further acts in the TOM40-specific pathway after the action of the MDM12-MMM1 complex. Essential for establishing and maintaining the structure of mitochondria and maintenance of mtDNA nucleoids.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. Component of the ER-mitochondria encounter structure (ERMES) or MDM complex, composed of MMM1, MDM10, MDM12 and MDM34. A MMM1 homodimer associates with one molecule of MDM12 on each side in a pairwise head-to-tail manner, and the SMP-LTD domains of MMM1 and MDM12 generate a continuous hydrophobic tunnel for phospholipid trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <text evidence="8">The ERMES/MDM complex localizes to a few discrete foci (around 10 per single cell), that represent mitochondria-endoplasmic reticulum junctions. These foci are often found next to mtDNA nucleoids.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the MMM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1M9"/>
    <dbReference type="FunCoup" id="A0A1E5R1M9">
        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1M9"/>
    <dbReference type="InParanoid" id="A0A1E5R1M9"/>
    <dbReference type="OrthoDB" id="5599157at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21671">
        <property type="entry name" value="SMP_Mmm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03103">
        <property type="entry name" value="Mmm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027537">
        <property type="entry name" value="Mmm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019411">
        <property type="entry name" value="MMM1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466:SF0">
        <property type="entry name" value="SMP-LTD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466">
        <property type="entry name" value="TEX2 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10296">
        <property type="entry name" value="MMM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="topological domain" description="Lumenal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="8">
        <location>
            <begin position="60"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="SMP-LTD" evidence="11">
        <location>
            <begin position="120"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="269"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="294"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ80809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="384" mass="43973" checksum="4105EB983146627F" modified="2017-01-18" version="1">MASDEKLNHMVHEYVRRVPVSTIQAGSSSFDTWSFTKGLIIGQISVILVFIFFLRFFIFSDSRSPNPRNSLSIFSKLSKSSTIIKLGGKERVVEEELESDLEKKLHSILEKTYYDVDTHKPESLDWFNVLIAQTIQQFRLEALLKDNIVHSLNSFIEGKSGDIPDFIDSMKITELDIGDDFPIFSKCRIQYSPNSNKKRLEAKIDIDLSDRLAIGVETKLLLNLPKPLSASLPVELTVSIVRFQACLCVSLTSGEEFVKANSINNPTFEFEDDTTSSKSSNKKPKNSYNGLDKPEDDDNDDDDDEHNGGYYLMFSFMPEYKMEFEVQSLIGARSKLENIPKIADVIEYQIKKWFVERCVEPRFQFVKLPSLWPRSKNTREEVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1Q0</accession>
    <name>A0A1E5R1Q0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 13">Cytochrome c oxidase subunit 6, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 13">Cytochrome c oxidase polypeptide VI</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g3870</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of a catalytic core of 3 subunits and several supernumerary subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Mitochondrion inner membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytochrome c oxidase subunit 5A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1Q0"/>
    <dbReference type="FunCoup" id="A0A1E5R1Q0">
        <property type="interactions" value="488"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1Q0"/>
    <dbReference type="InParanoid" id="A0A1E5R1Q0"/>
    <dbReference type="OrthoDB" id="5778907at2759"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00923">
        <property type="entry name" value="Cyt_c_Oxidase_Va"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.40:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.40">
        <property type="entry name" value="Cytochrome c oxidase, subunit Va/VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003204">
        <property type="entry name" value="Cyt_c_oxidase_su5A/6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036545">
        <property type="entry name" value="Cyt_c_oxidase_su5A/6_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14200">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14200:SF11">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 5A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02284">
        <property type="entry name" value="COX5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48479">
        <property type="entry name" value="Cytochrome c oxidase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0349">Heme</keyword>
    <keyword evidence="8 13" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 13" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00082700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ80820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="156" mass="18079" checksum="F0CEDE03E229863A" modified="2017-01-18" version="1">MLSRVLFQRPAIRLSSVATSNVPRMAMTMSLRQTPQQHNIPSVASRFYSSASGTGHDEETFEEFTTRFEKEFDQAYDLFEVQRVLNNCFAYDLVPAPVVIEKALRAARRVDDLPTAVRVFEALRYKVENEEQYEAYLHELEDVRKELGIPLKEDLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R248</accession>
    <name>A0A1E5R248_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative phospholipase, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4068</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R248">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R248"/>
    <dbReference type="InParanoid" id="A0A1E5R248"/>
    <dbReference type="OrthoDB" id="69269at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004177">
        <property type="entry name" value="DDHD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058055">
        <property type="entry name" value="PA-PLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057826">
        <property type="entry name" value="WWE_C20G8.02"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23509:SF10">
        <property type="entry name" value="LD21067P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23509">
        <property type="entry name" value="PA-PL1 PHOSPHOLIPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02862">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23463">
        <property type="entry name" value="WWE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01127">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51043">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDHD" evidence="2">
        <location>
            <begin position="594"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="404"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="814" mass="93487" checksum="8C7F4E7E2B4253B9" modified="2017-01-18" version="1">MVIAMVRVFAHAKVGRLFITVRYSSRSVXNSQNQLPFVSWYYATDKPKRQSFRDLDDGNDILTQKSRSSSGKQKQLEVFLQNEEYYKKPAKKFKPFNKQDSANLEKAYQDYISHMQKVELRKGVLITNNSFDSQSQYTVQVNEDRLFQVDVHKWELEPVYWEGAVYEVRRGAWFTPDKTPVNASVMIQLESHYHKLHQSQTREKGKSQGTKHLSNKPSASTDSNSFIPLYKTLEYCVDYGLDPAIQKNEHEQLVRKLPKRKPTHYLYFNDQDLRDETVFVIPFDQKSYYSGGIALNLLRNGIITIGVTKLSRNINSTEQEKQQLQIDSTKSEKNKEEKLKKEAYSVFGGLIPESVSNLVSTNWASVLGVSFMGSKKTSIEEQKQKEQEAAEKTMKEEIEKDFDTSNTDDNSETGSASNERQIDHLVFCIHGIGQTLGRKYQYVNFSHTVNVLRKNMKTLYGTTEKFQKLNENDKKNSRIQVLPITWRHRIGFNTEDLDTNRNIAELPTLLDLTLDGVRPIRKLIGDIALDILLYDEEYYQEKILKEVISELNRIHELYCRNNPNFKGNISVIGHSLGSLIFFDILSKPELAKQLNFDVVNFFGIGSPVGVFKLIQRTMIGDPALAFNQKNGMHKRADPSLVRYANLACENFYNVYHMCDPISFRVEPLVDRRMAHIEQESLHTLSATNQIAAKVLEISESLPSPNASFTTVKDQLLARVRKTDGVHLDYHAHSNNTVTSKIALNKDLLEKLLQVNKKNGRVDYSLKSGLLEMDVINAIKSHVSYFEDLDVSGFLMTELLKKPTLVYNKHVELKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R257</accession>
    <name>A0A1E5R257_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative drug/proton antiporter YHK8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3912</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R257">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R257"/>
    <dbReference type="InParanoid" id="A0A1E5R257"/>
    <dbReference type="OrthoDB" id="9986881at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140115">
        <property type="term" value="P:export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042908">
        <property type="term" value="P:xenobiotic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000082">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF7">
        <property type="entry name" value="DRUG_PROTON ANTIPORTER YHK8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="299"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="377"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="407"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="459"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="485"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="550"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="145"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="58"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="586" mass="65254" checksum="491B1719C19612B2" modified="2017-01-18" version="1">MSXPSXAFEQSDSVGSSVNEFPITEQKGSSPNEKINDCDNSEDYADSVQNFEKXINLTNTTDHAGNPVNSNSSNSRNHKNSQRQENDAYSMYPDEGLLDEEMSRVSTNMREENRSLPNESFEVTLGPNDPENISTRISYLRKCYFTAIVSYXSVVITILSSVWTFAAPIEMERFHVGQEVATLGITLYIVGLGFGPLFLSPISEFYGRKITFIFSLFFVLVFIIVTAFSPTLAGIFIGRFFSGFFGSAFLSVSGGVVSDIFSKEDIGIPMSVYSISPFLGPTVGPLISAAFVHNNEMKYAFVTLLGCTALSLFLIVFSFPETYKPILCCQKAARLRKELXDKRYYAPLEIIRKETKFVNAVLLSSKRPLLLLAKDPMMAVLCVYTGLILGIVYMFFVAFPLIYKEKIYHFTAAETGCTYLGILVGTLLMTATSPKIQKRYGAKVQANNNVSVPEYRFEALFYGSILCPIGLMIFAWTTYSHVHWIGSIIGNVIFGAGASYIFAGILGYTVDAYRLYAASAMAANSFVRSIMAGVLPLITRYMYDRMGINWASFFLAMLCVLMIPVPFLFTKYGAVLRFKSPYAWDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R264</accession>
    <name>A0A1E5R264_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcription initiation factor TFIID subunit 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4185</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R264">
        <property type="interactions" value="246"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R264"/>
    <dbReference type="InParanoid" id="A0A1E5R264"/>
    <dbReference type="OrthoDB" id="28335at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08048">
        <property type="entry name" value="HFD_TAF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045127">
        <property type="entry name" value="TAF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006809">
        <property type="entry name" value="TAFII28_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13218:SF8">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13218">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04719">
        <property type="entry name" value="TAFII28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TAFII28-like protein" evidence="7">
        <location>
            <begin position="196"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="294"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="406"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="308"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="413"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="8">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="495" mass="56109" checksum="601168BACD4C1E95" modified="2017-01-18" version="1">MSEFTTLIEKLNESQFQNIVSLSYYIGVKQIVDQLLSEDDDYCQWKLQQYRTGENSTMGDLIEEYLSTTTEAANEYXVDGKEIGQEGEEAYEXGILGFGHEXFIKKHNLPFSTQSEETEFARDAKLAGNTLNADDTAILKNNNHNNDEAVANECIEHDITTIPDDLIFPKELYESIIKQNTDAVKELSEPEKLKLLGDNLDTAQMDRFELYRRSKLPKTLVRRLIGTVTNQSVQTSTALYLCAVGKIFLGEIVEKALHIKKKSLISHSFRTLNLRKVYGNRILKILKKITKSIENSSNNSGPASFKPTSKSGSGTVNNSSSMNSSLSNKLQYNLGNDDLDYLFNEDHINCDTIFPTEDNELMENNDLVEDDYYENIKDTKQQNLKTMLKNLKALLENDYTINASPTAEKPLLSSSSSSSSSSFSSSQFGKLKSLVIQYNFWLEKFNALDCNIDIFENSPLQPEHVREAWRLYRQESDTLPPSAYRLQGEGTGWLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R276</accession>
    <name>A0A1E5R276_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribosome-associated complex subunit SSZ1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4015</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R276">
        <property type="interactions" value="431"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R276"/>
    <dbReference type="InParanoid" id="A0A1E5R276"/>
    <dbReference type="OrthoDB" id="29851at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000010">
        <property type="entry name" value="heat shock 70 kDa protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF32">
        <property type="entry name" value="HEAT SHOCK PROTEIN PDR13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="476"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="476"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="494"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ80997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="566" mass="61904" checksum="4A2F6B656E5D98D6" modified="2017-01-18" version="1">MFNPIVGISFGNVSSSIAIVKQDNKGTVEVIANQDGERTIPSALSYKGDDEYHGGQAIQQLVRNPKNTIINFRDFLSFNKFSEIDSQLIKDRTGSGNGCELVDIGDDKPGFILEQGKTKLSVXEVSARHLKNLKLAAEDYTGITLEKCTLTVPVDFNEAAKKNMAQAALKAGLQVVQFIAEPTAGLLSILESSQATSHSILVNKREQKDKNVVVADFGGFTSTVSVYSIRNNGILSSLHTEINHKLGGENMDDALIKFLAADFEKKTKTKCLNNIRSLAKLKINAILTKKTLSNVTNATVGIDSLADGLDLHTSINRLRYELVISKIVNEMASFVESTIAKTGLDILEIDAVVLLGGASFTPKLTVNLQDLFSDGECEIISLTSGNESQHPDEMISAGAAYQAALLEEDDTEALETLVEHAVSVPHLNQTIGLVNAEGAFEPIFEKNTPLPSRKSFKLYKQQKDFQLELYTGKVEIKETVEEPPAKEEKIEQDNKDDDESDWSDDEDDEPEVIREKVYAKQDKLLSFGGKGGKKGCELIFEISKDCGLKIVVRDLSNGEVTSKAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R279</accession>
    <name>A0A1E5R279_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ubiquitin-conjugating enzyme E2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g4261</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="5">Protein modification.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R279"/>
    <dbReference type="FunCoup" id="A0A1E5R279">
        <property type="interactions" value="261"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R279"/>
    <dbReference type="InParanoid" id="A0A1E5R279"/>
    <dbReference type="OrthoDB" id="19692at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23811">
        <property type="entry name" value="UBCc_ScCDC34-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000063">
        <property type="entry name" value="CDC34p Ubiquitin-conjugating enzyme (E2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="9">
        <location>
            <begin position="7"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="196"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="196"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="245"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="331" mass="36122" checksum="C8AA25B3947B4520" modified="2017-01-18" version="1">MSTKKSTAASLLLRQYRELTDPKKAIPSFHIELADDSNIFLWDIGVMVLNEDSIYHGGYFKAQMKFPEDYPFSPPQFRFTPAIYHPNVYRDGRLCISILHQSGNSTSDEPDNETWSPVQTVESVLISIVSLLEDPNINSPANVDAAVEFRKNLDQYKGRVKLEVERSKQDIPAGFVMPTSSLHAYTSAANIHSNKTTFGKNPTATTHNDSSASLSSASTGMNDIRGDNANSGGDKFSNRSSMGFGNTTNAAEGNNNKDNNNEIHNASPGASASSTSGIANESNNNSADNFWYDSDLDDEDDVEMVFYESTDDEGDEIDAADAEVATGKAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R284</accession>
    <name>A0A1E5R284_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Large ribosomal subunit protein mL67</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Mitochondrial homologous recombination protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3946</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL67 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R284"/>
    <dbReference type="FunCoup" id="A0A1E5R284">
        <property type="interactions" value="165"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R284"/>
    <dbReference type="InParanoid" id="A0A1E5R284"/>
    <dbReference type="OrthoDB" id="5333655at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024629">
        <property type="entry name" value="Ribosomal_mL67"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28184:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML67"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28184">
        <property type="entry name" value="MITOCHONDRIAL HOMOLOGOUS RECOMBINATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12829">
        <property type="entry name" value="Mhr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00035511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ81016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="232" mass="27267" checksum="26897623B1009180" modified="2017-01-18" version="1">MPVVSSSSNSFRTARWLQKAGYAPQVFVFRNLESGQVCYSQLPTINPRYVNKLQFKQCNKLNRTPDFKRRDIWKAMCVINLTTYKDSIQVFQNLNRLRFFRDVQYKKQNDELRKKHCGNVFQDGRYAPVFAQEAVADIKESLLKSGIDFSKLTGNEVTLHWEDEWRKGDLNMFWNKDLPQVKHEMIERSLNTGREESVILKKLGDLAKLNWNVTDDDMVFKKMLNKETKVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R294</accession>
    <name>A0A1E5R294_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">G2/mitotic-specific cyclin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g4318</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin AB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R294"/>
    <dbReference type="InParanoid" id="A0A1E5R294"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044772">
        <property type="term" value="P:mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20512">
        <property type="entry name" value="CYCLIN_CLBs_yeast_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000005">
        <property type="entry name" value="G2/mitotic-specific cyclin B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048258">
        <property type="entry name" value="Cyclins_cyclin-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00292">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 5" id="KW-0195">Cyclin</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="7">
        <location>
            <begin position="482"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="8">
        <location>
            <begin position="575"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="7">
        <location>
            <begin position="579"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="76"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="131"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="199"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="76"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="213"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="240"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="251"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="267"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="278"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="308"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="368"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="708" mass="79553" checksum="AF883546A0F4A9FB" modified="2017-01-18" version="1">MSSYTNENKEHASQDRMRSHVNAKQSYLKTAMSLPSXFLKRSALSNVTNTTVGPSSANNAYHIKNGNKMLSNNDEFTGSGNTHESLXTNSFHNKENINSNNRTNSXDIANTNNNRTNAISXTAIENDNTNVQLNSQDDNRGNIPASRSNSYTNVIPEGTALKPSVIEATGLISQTRHIANSSKINGHSTAISELQDAIQVSKQQPSHQPGAPSSSSSSSSXTTSSTTATATTTATIAHSPNEDHVMEDTSHLSQDQELLSHSSIRKQPLEIIREDETSQAVMGQSSRIASSSSNEQNERGSNMMTGHNGSNNINENNSGVHLLXNNSNHTINNNRFEQTGKRPISTLVDSSELKKFKPENNVPLENYQSSASSSHSNNSQNNAHQSSDQNTNEKQQQQVKNELRSSGIXTEVVQQSSRPNTVYYEYEWEDLDAEDSNDPFMVSEYVVEIFEYLHKLEFQTAPVREDLFKHPNINQNRDILVNWMVKIHNKFGLLPETLFLSLNIMDRFLCKERVQLDKLQLVGTASLFIAXKYEEVYSPSCKHFAQETDGACDEEEIKEGEKFILQTLNFQLNYPNPMNFLRRISKADDYDIQSRTLAKYLLEITVVDFRFVGELPSKCAAAAMFLSRKMLGKGYWDGNLIHYSGGYTKMQLESICLMMMEYLEQPVVHDEFYKKYASRKFMKASVISRQWAKKVISQGYNVMSLHEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2G4</accession>
    <name>A0A1E5R2G4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3902</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2G4"/>
    <dbReference type="InParanoid" id="A0A1E5R2G4"/>
    <dbReference type="OrthoDB" id="25896at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="487"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="590"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="677"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="499"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="590"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="605"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="619"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="677"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="725" mass="81618" checksum="473FEA44FD3CC18E" modified="2017-01-18" version="1">MTTVLFDYTNRGASALPRCYDISSVRVLVVGDRGVGKSTLIENLFASSNIKNLHGQTHEHGFQGFHGFHEVRGNRVFGENGCNSEDLIHKKTDYTNLVQSKNFKEFKDKDINCKCLFSDFASLHENDGEGMEESINHTHSHIHNEHNXLHVDGDFGDHHHEHMHTGHSGYKFLQQFPQIFVKDYSKMKFNCDLSLDVQIVETSNESFQNYFSDIKRVQIHQTDIFLLCFDPTQPQSLENLKTTFSILITNLDPMEQSYEYSIYFVCTKADNIHYKNYYLQIKEFLQNFKLNILKNYYVVSSKTGYLMRHPVVENSGFDSEDNEDAVMDESEDDNRSEEDQDDFENGGSDIDFAVSIGSPRSNNRRQSRSQSSFSPLLHHPKHTRSQKVLSVDELFFRMLKNYEAWRSYCRNGTWDAGVFTSSTNTITSGTLRSASPDSSARYSSIYPTSVYMTASARNTPTLSTSDSANNSNSNIALLNHTASFVSAPAVPTHPTSMISDNNDTNSNNNNNKDNNNNNNNNNNNXNNNNNNNNNNNSATNLQESSTKSLFVVSSDVKKSSFSSSNILEQKQAAMGNNRVAEIKSPVPIQLSKQNSLQRTTQELENETHSQEXKGHTQENKTPVQGNLENKTSNTDKINNTSFDFAPEAQEIALGKAQTSEQPKNTILPVTSNLKKFPKTTSXKEISSSNGSNAHKSIQQNIRKTKPPXKPPTKQKSDRGGCCIIX</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4K5</accession>
    <name>A0A1E5R4K5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3698</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R4K5"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="413"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="519"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="413"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="519"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="600"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="648"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="881" mass="96363" checksum="48B4F8FAE8D2333B" modified="2017-01-18" version="1">MSITANQHEMQSNKSQNAIPQTNSSQIPNTEWNCRNTLHNAFNTIKQHFIENRASFFLNKDGLPKEDVLENANAAENFASCPMAIEANSWSPQLTQSGSRITQSNLRIAQLSSSFTPITSGIASQGAFKFAFKSAFRWNYVSLGLANAALGSEYASAGATKGIRNFTETRSQGGQFSSSSSPILQMIEKERAAKKSKRARMRGAKAALKRKLHKSKAALKCKLHKSKSELKCKLHKSKTELKFKFADRGLFKACGKSAQAYEFACKRVQPAESLRAGDSLESRGSNTNDADPNSETALPVSSTSASDYGAVPNDGFSEQETXGNKKAVKRSKRARIRDAKAAFKCNVQHSKARFKCKLSKCSFLKVNKDHYQANEDSNERGRPDELLREIDTLTSNGVDIDSTEEAAGLAMLDNSTSDASPDTPSTNILDISAQGSPSNTQETQASTLATEDKTSEGELVQQIIKKVRFILPERNYSDDKKTLAHAYXFAPLGGSNTTSNSNFAFCNIGYTVEQELEQNAADDDEADDEQDAADEDAADDDQQNVAEEDAADDDQQNVAEEDAADDDQQNVAEEDAADDDKQNVAGDGEQNVAGDGEQNVAEDGEQDAAEDGEQDAADDDAADDDTADDDTADDEEHEICEAIKAGSYSXSRENDYSGSKKTLAHTSNITSLGGFNTTXIPNFAPCSIXYXVXQKLEEDTDNDEEGVDNDADDYEFYEAIKARRYSISRENDYSGNKKTLAHSFAPRSIGYSVXQELEEDTDNDEEGVDDXEXLDDDXXXEFYRALKAGSYSKSRENDYSXNKKTLAHTSNITSLGGFNTTSIPNFAPCSTGHTAEQEGEVLEESVKNPNFQPFHNPIPDIEKYFRGWGASLNSTPPLKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4U6</accession>
    <name>A0A1E5R4U6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3784</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4U6">
        <property type="interactions" value="112"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4U6"/>
    <dbReference type="InParanoid" id="A0A1E5R4U6"/>
    <dbReference type="OrthoDB" id="20109at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000171">
        <property type="term" value="F:ribonuclease MRP activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034965">
        <property type="term" value="P:intronic box C/D snoRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013241">
        <property type="entry name" value="RNase_P_Pop3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28272">
        <property type="entry name" value="RIBONUCLEASES P/MRP PROTEIN SUBUNIT POP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28272:SF1">
        <property type="entry name" value="RIBONUCLEASES P_MRP PROTEIN SUBUNIT POP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08228">
        <property type="entry name" value="RNase_P_pop3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ81888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="231" mass="26584" checksum="2E218CDF84EA4A40" modified="2017-01-18" version="1">MSLKSTQNIIATKKQVYKPILDNPYTDIATFPHVNSQHEIKSLLELSVLKTFKQFWQMQQNNQQQLSSRTCPVDIVFGFNKVMQYLESNIDEPCFVFVCNKDGLTKILHQHLPMLVANRNSQPNKQHMXRLIQLPKSSLDMFNANLPLRSSTLDHEDGDYHYGILIVKGENIPGSLRIHVHTNVPDVSIPYLETSYVPCSENLHLLSTSMSLEKKERKGKVLKKKKNMIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4X0</accession>
    <name>A0A1E5R4X0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3611</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4X0">
        <property type="interactions" value="197"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4X0"/>
    <dbReference type="InParanoid" id="A0A1E5R4X0"/>
    <dbReference type="OrthoDB" id="40579at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.240:FF:000001">
        <property type="entry name" value="Haloacid dehalogenase-like hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901">
        <property type="entry name" value="2-DEOXYGLUCOSE-6-PHOSPHATE PHOSPHATASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901:SF38">
        <property type="entry name" value="PSEUDOURIDINE-5'-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ81918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="244" mass="27382" checksum="1F0966A5B3D1BFCF" modified="2017-01-18" version="1">MTRDXKVIRACLFDMDGLLINTEDIYTETQNELLSRYGKGPITWDIKVQLQGLPGPEAATKVIKKYDLPLDLIQYEQEAVEIQTARWGTSAFLPGAKELLHFLKKEQHLPIVLCTSSGKAKYLSKTDHLREDFDLFDAIITGCDSRIPKGKGKPAPDIYQLGLAELNAKFHKDGKDLIKPEECLVFEDGVAGVLAGVSFGGYVIWCPHPEAADHLPTPESIMYEQGEKVDTLNHLDKSKFRFQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4Z1</accession>
    <name>A0A1E5R4Z1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA damage tolerance protein RHC31</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3509</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4Z1">
        <property type="interactions" value="1201"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4Z1"/>
    <dbReference type="InParanoid" id="A0A1E5R4Z1"/>
    <dbReference type="OrthoDB" id="1708823at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031510">
        <property type="term" value="C:SUMO activating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019948">
        <property type="term" value="F:SUMO activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF162">
        <property type="entry name" value="SUMO-ACTIVATING ENZYME SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="1">
        <location>
            <begin position="19"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="5"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="350" mass="39345" checksum="69A5FB8FB6DFB1A9" modified="2017-01-18" version="1">MSDPDAVKLSLSKEEIELYDRQIRLWGISAQQRIRASKVLLYNLGGCGTEITKNLVLSGVGSLTIYDSHLLSEDDLFNQFFISKDDELDVPRLQHAKPRIQDMNPRVEVNCVSSTNLLNDLDAIKNYDMVIVTECNDAQTIEAINNTSREFKIPFYLCGGNGLYGYCFIDLIEFISTDEKLKNGNIRTRPGKLSENKEILEVVERTDEEDXGKVYEVIRTKNIYKPFCDMLKFDPLTLSQQMTKRQLRKITNALPLTLANFNAKGTNISAATAQQVAQELGLPVSDSYNLSADFIATFNRMKNIEYPPVNAIIGGAVAQDVINVLGKQESPLNNFLVFDSNTLEMPILEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R570</accession>
    <name>A0A1E5R570_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">AWRI3579_g3660</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>protoporphyrinogen IX + 3 O2 = protoporphyrin IX + 3 H2O2</text>
            <dbReference type="Rhea" id="RHEA:25576"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:57307"/>
            <dbReference type="EC" id="1.3.3.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protoporphyrinogen/coproporphyrinogen oxidase family. Protoporphyrinogen oxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R570">
        <property type="interactions" value="777"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R570"/>
    <dbReference type="InParanoid" id="A0A1E5R570"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00324"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004729">
        <property type="term" value="F:oxygen-dependent protoporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065003">
        <property type="term" value="P:protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022613">
        <property type="term" value="P:ribonucleoprotein complex biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03758">
        <property type="entry name" value="proteasome_beta_type_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12246">
        <property type="entry name" value="RRM1_U1A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000001">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000007">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000615">
        <property type="entry name" value="pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035206">
        <property type="entry name" value="Proteasome_beta2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004572">
        <property type="entry name" value="Protoporphyrinogen_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00562">
        <property type="entry name" value="proto_IX_ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF85">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 23" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="11" id="KW-0647">Proteasome</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="21">
        <location>
            <begin position="720"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="22">
        <location>
            <begin position="906"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="20">
        <location>
            <begin position="1948"/>
            <end position="2025"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="494"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="620"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="625"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="640"/>
            <end position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="OEJ82044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="2031" mass="229134" checksum="6CE8C8BC2F5EC90D" modified="2017-01-18" version="1">MDIVLGIRVKDAVLLASSKAFTRGISILKDSDDKTRALSDHCLMSFTGESGDTTQFAEYIQANMQLHSIRETNNNTNVQETSTTSDEGSIGTFELSPSATASFVRNELAKSLRSRKPFQVNVLIGGYDTKANQPLLYQIDYLGTKVELPYAAHGYAGFYTFSLLDHHYRPDMDVTEGLKLLQKCLDELVKRMPMDFKGVYVKIVDKDGIRLLINSXGGRXPTLLKAKVYLDEEXEKXVHSNKSRFEELGHFLFWMNNCDVKTKLSAFLKLNDEEVIDYILASFNKLQDEKNFVARLIDLGIQESKCKFVYKILDANKQANGQVVVYNTIDDILATTSLPKDSLQTVAXFIENIFTKHSSKVEFVNNLXELDLDTTISEQLFHFLTAKTYKKSKLVSNNTNDVVVIASSDLEPVVNKVYKCIITGKTTYGXFCRVLGVSHEPEAMCHKSELPWKTYIEQFNINDSVFAKXLRMQPNGKIAVSMRNVDQMTGKPLQDIQRGRTDQRSPSVSAVEEKKLTSPERWEISKLISSGAAKLDDYPEYKNFIQKDNQKASTQASLVNEGLPGFKHQLGKKRNQLNELAPGEDIEIEFNKNSTPAFIKKNETLKTSVRKYQLEPLTKIPKGSMNRNASSGSALVTNFRNEKADKKKELAQQKKSDHSQRKEPLKLTGSNNIDIGQINALTAWERKKMREKIEYGKRTNLSLKEQRESLPVYKMRQDLLDAINGNQFXVIVGETGSGKTTQLTQYLYEEGYNANGLMIGCTQPRRVAAISVAKRVSEEMECALGGKVGYTVRFDDQTSRDTKIKYMTDGILQREALLDPLMSKYSVIILDEAHERTVATDVLFCLLKXAALQRPDLKVIVTSATLDSKKFSEYFTNCPVLKIPGKTFPVDVFYSKAPQMDYVEAALDTVMEIHINEARGDILVFLTGQEEIDACCEILFERVKNLEDTLDLKLIILPVYSALPNEIQSKIFEPTPPDSRKVIFATNIAETSITIDGIHYVVDPGFSKVNTYNPRLGIEQLIITPISQAQSNQRKGRAGRTGPGKCYRLYTESAFLNEMLPSTVPDIKRQNLSNTILLLKAMGISDLLSFEFMDPPPKSAMVYALEELYHLQALDDAGQLTPLGKKMSQFPMEPLLSRTLLASIELKCSEEVLIIISLLSVQNIFYRPKNNQIEADRKRMNFAHPYGDHLTLLNVYNKWKVVSSTSSFSRNKVSTTKWCEMNYLHERHLKKSKDVYDQIRKIFDKLDLQTISCGPNIDNVRKAFVFGFFRNSAKKKSGNTYTTIVDNTEATIHPGSVLFGKSCDYVIYHSLIMTTTEYMSTLTTIDPKWLCECAPHFFKAHVMNNKKMKIESLDNRNGDKNEWRLSSLRRKKESLLSNMFWSFMKLIXNKARRAQKTKLTSMTXLALHKKLAPNSKVAVLGAGLSGLTFAYFLNRLRPDVQLTIFEQKPKSGGWINTDRVNFGDLAHPVKLEKGPRTLRGVSDGTCMLVDIMMNLGCKQDIMCIDSKSSANRKFLLDKSQNLLQVPPTTPASFLKFLTNPLSSGLFMGICKEPFAPQKSISAGDESVYDFLKRRFGTASIADNLLSAVFHGIYAGNVQKLSANYTLKSLVNMEKEHGSIVKAMFSKMQNSKETAQNETKLSGVLQKYGSSTSSNINELQKLLKLYPMLGFENGLSTVPDAIETHLAAQENVSIQYGSNLESVEASPSSKKKLIVNGEPLHFDHLHASTVPSNLRISDDLTKFLKTITEVNVALVNIYHPKKDLLGSNIHGFGYLVPRVVDNPQHLLGVIFDSVIENNFLPLFSNQQIEKSTLNYTKITAMLGGHYYNNQIIPSEKIIIQQVKDCLAQHLHFSEKDLNDCKFEFTLAKNSIPEYYVGYDSVPVEEKVAELGVSLGGMKFASGPGLPDCVLLVNRNLLQVYFQYCEVAKPKPKHFRLEQGMSKQESLPTHTLYIHNLNWKIEHGKLRENLYLIFSTYGEVLNIYMNHKLREQAFITFKSVDEANFAKISLNKEMFFGKPLHIEYSKIKNEGAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5D5</accession>
    <name>A0A1E5R5D5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcription factor SPT20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3840</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5D5">
        <property type="interactions" value="413"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5D5"/>
    <dbReference type="InParanoid" id="A0A1E5R5D5"/>
    <dbReference type="OrthoDB" id="1932706at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021950">
        <property type="entry name" value="Spt20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046468">
        <property type="entry name" value="Spt20-like_SEP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13526">
        <property type="entry name" value="TRANSCRIPTION FACTOR SPT20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13526:SF8">
        <property type="entry name" value="TRANSCRIPTION FACTOR SPT20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12090">
        <property type="entry name" value="Spt20_SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spt20-like SEP" evidence="2">
        <location>
            <begin position="151"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="442"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="240"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="442"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="463"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="487"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="499"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="509"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="540" mass="61267" checksum="37749EE0A69B20F2" modified="2017-01-18" version="1">MQVNQGISTGSSEHQAQARQSPPVSAGSMGSPQSSTNNPMNMTLQMQQLQQHQAKTLQQSTPLNQQQLTPQQKQQLLVIAQQLRVSPQQVQQLTPLQVHHMQQKMRQHQQRQNAISLEQQFNMVMGSVYPKPKRMYKFVEDSDSILKKYEKFMQSFELHIYESAYKICPPNKSRSGSYRQEFGGDGLTLSKNDETLKDFLEYVARGLIPASLIDILADSNIQFYEGNLILQVFDHTNTTDVTPPTKKPVPTQAEERANPNQTDASEKQQVAKQSLLKRPRIYRTLLRPNDLTTYYDMLTMSDEHKLSDAIYQNVESEILNLTKRNLXLSVPHNPYESFNAHLEEKLGLDMQEKANNRKLCGDPNSRGANPHTEMVYKQPISSYEEMMLQYSAPVQRKKFLGFAVDFKNSSAGKALLARMANQPQLLKEQQQQQQQQQQQQQQQQQQQQQQQGSDGKPQETKKKAPAKPRVTKPRAQNKGAPRKRQVKPKEDKPKDEKPKVRRPRKKKGAAAAEAEATAAKADPGMVPGEPKPTGSAPEPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5I3</accession>
    <name>A0A1E5R5I3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Phosphoribosylformylglycinamidine synthase</fullName>
            <ecNumber evidence="4">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Formylglycinamide ribonucleotide amidotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Formylglycinamide ribotide amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">AWRI3579_g3622</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the FGAMS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5I3">
        <property type="interactions" value="976"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5I3"/>
    <dbReference type="InParanoid" id="A0A1E5R5I3"/>
    <dbReference type="OrthoDB" id="6666987at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01740">
        <property type="entry name" value="GATase1_FGAR_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02203">
        <property type="entry name" value="PurL_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02204">
        <property type="entry name" value="PurL_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.10:FF:000002">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.10:FF:000005">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000008">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.650.10:FF:000002">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.750">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase, linker domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00419">
        <property type="entry name" value="PurL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040707">
        <property type="entry name" value="FGAR-AT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055181">
        <property type="entry name" value="FGAR-AT_PurM_N-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010073">
        <property type="entry name" value="PurL_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041609">
        <property type="entry name" value="PurL_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036604">
        <property type="entry name" value="PurS-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01735">
        <property type="entry name" value="FGAM_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003672">
        <property type="entry name" value="PRK05297.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10099">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10099:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18072">
        <property type="entry name" value="FGAR-AT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18076">
        <property type="entry name" value="FGAR-AT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22689">
        <property type="entry name" value="FGAR-AT_PurM_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13507">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01211">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109736">
        <property type="entry name" value="FGAM synthase PurL, linker domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82697">
        <property type="entry name" value="PurS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribosylformylglycinamidine synthase N-terminal" evidence="21">
        <location>
            <begin position="39"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosylformylglycinamidine synthase linker" evidence="20">
        <location>
            <begin position="197"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="19">
        <location>
            <begin position="462"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="FGAR-AT PurM N-terminal-like" evidence="22">
        <location>
            <begin position="679"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="19">
        <location>
            <begin position="858"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="329"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00052585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00057317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00071729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF18072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF18076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF22689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="OEJ82159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1338" mass="146560" checksum="FE694406023005D4" modified="2017-01-18" version="1">MTVLILPGPQALSAFRVDNLVKKISSSNAVSRDLVSSCFVHYVELQQNAELTEKELGLLNSLLIYDSPCLDSNLNTIVSQNTSTDSETLIRVLPRTGTISPWSSKATNIAHVCGLAGKINRIERGLAIHIKSYKPQDLTLNEDSLKSVFDRMTQSLFVNEVPNKEDLFNHESARPLVHVDLLAAQNPXSTQTXYDILKQKNVELGLALDEGEIQYLINAFNQRNPTDVELFSFAQVNSEHCRHKIFNADWTIDGELKNLTLFKMIKNTFNHCPDYTISAYSDNAAVLDCDHPEDKQFYYAPDFQTKEWKSTLEKVHLLIKVETHNHPTAVSPFPGASTGSGGEIRDEGATGRGSKTKCGLSGFATSDLLIPNHKQPWELDVGKPSHIASALDIMIEAPLGSAAFNNEFGRPAINGYFRTLTTEVKNSEGNPEIRGFHKPIMLAGGMGSIRPQFALKNKPITPGSHLIVLGGESMLIGLGGGAASSVASNDDNVALDFASVQRGNPEMERRCQEVINACVSLDKINPIQSIHDVGAGGLSNALPELVHDNNLGAVFDIRKVLSLEPGMSPLEIWCNESQERYVVGVADGHDLEVFTNICKRERAPYAVVGVATKEEKLVVEDPLLKTTPIDLDMSVLFGKPPKMTRTAITQDLQLTKPDYSQIQLNDAIERVLRLPAVASKSFLITIGDRTVTGLVDRDQFVGPWQVPVADCGITATCLSSDYINQPGEALSMGERPTLALISGAASAKMSVAESLLNILSADVKSLKHVKLSANWMSPASHMGEGSKLYEAVQAIGMDLCPDLDLAIPVGKDSMSMKMKWGKKEVTAPLSLNITAFAXVNSTSNTWTPELANKTAPLVLVDLGKSAMGGSCLLQVYNQLGDEAPTVYNNKQFKGFCEAIMELHALETSVVGAYHDRSDGGVLVTLLEMAFASRCGLKINASANKSGNLIADLFNEELGAVFQVLDMAKFKQVMNNHGIXDDLISVVAEPLQDSLNVEIVDGQENQVVYKAVLNDLLSAWSETSYLVQELRDNPDAAKEEFGNLKDASNPGLFYDLTYSPAEPLNIADSKKDKPKVAILREQGVNGYVEMSFSFEQAGFAVYDVTMTDLIEGRFHLKDFVGLAACGGFSYGDVLGAGNGWAKSVLFNENLRAQFIDFFQVRKDTFAFGACNGCQFLSRLSELINPETPEIWPIFERNKSEQYEARVCMVEIVSDNQVFFKNMKGSKMPIAVAHGEGQATFASAQDLNNFESNNLCGVRYVDNYGATTERYPYNPNGSPSGITGILSPNGRVLAMMPHPERVTRLESNSYYPKDKYAEWAARGPWVKMFKNAREWVEGLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7H4</accession>
    <name>A0A1E5R7H4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Oligomycin resistance ATP-dependent permease YOR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g3200</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7H4"/>
    <dbReference type="InParanoid" id="A0A1E5R7H4"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18597">
        <property type="entry name" value="ABC_6TM_YOR1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18606">
        <property type="entry name" value="ABC_6TM_YOR1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000565">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001750">
        <property type="entry name" value="ATP-binding cassette transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000010">
        <property type="entry name" value="Multidrug resistance-associated ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF456">
        <property type="entry name" value="MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN LETHAL(2)03659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="204"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="350"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="430"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="870"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="916"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="984"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1009"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1098"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="205"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="568"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="872"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="1191"/>
            <end position="1442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="540"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="788"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="547"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ82847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1453" mass="164185" checksum="41ABB928A8849BD1" modified="2017-01-18" version="1">MVIKQEGLQPHQSPSSSXSSGTQKANEQIINLQNSISSASSAHSQAKHKKIAPVYTENNESWDMTQGEYDLIHRDLERVADSEIVPQKRLFSFFYSKKLPDIPQSDEERKFVPLKTWNLLNTVFFGWCFELLRVGYKRTIQPNDLMRINNEYMVENLYNEFLINWEESLERKRKRKLEGKKNPKYEHYSILIVLLKTFKWRYGYAIFSMIIANLAVAFTPLVSKHLIAFVAEKQVVPGLKINKGVGYAIATIILMIIIAIFSAQGMFNASLVGSQVRSILTKALLQKSFKLSPKGKHEFPLSKITTIATTDTSRVEIGFTEHPMIWGFPGAIIVTIYLLIYNIGPVALVGLGYFLVSLGVTVWGFTFVMGFREIANQFTDARVNFVRDIINALKIIKFYAWEDAYLKLIAEKRKREITQVKKMQSYQAVVIAYSVMVPTVASLITFLCLYQIKHGNQSAAAIFSSLALFKVLSAQMFIIPQALSSTVDALVGMKRVQLCLLAEEAEEGQENELPCLNGKNAIELDKCTYEWEIFEDVEESNSDEISDEKSLLESNDNKLEKHSNENGIKGNDGEKSIDKPVFNGLHDITLNIKKGEFIVVTGSIGTGKSSLLLAMTHLMKQISGEYRQTGDLLLCGQPWVQNATIRDNITFGSDYDEKKYKKVLDICSLKADLKILEAGDLTEIGERGINLSGGQKARINLGRTVYRSADIYLFDDILSAVDAKVGKHIMEKCIMGHLKNKTRLLATHQLNLIKDADRIIFLGTDGSLTIDTMDNLLLSKLEFANLMANATTSKKHNEEDKLENEKEEIKKEEIQLINVKSEKDEISVEVAYDERQALVRGQLIDKEERAVNSIGFDVYKRYLVAGAGSFWLIATCLFFFADICNTFMDLFASVWLSFWNEMKWKNRSKGFYMGLYALWTVLSFFLTLGECLIILKVGLKSSKNLHNSAIKRVLHAPMSFIDVTPLGRILNRFTKDMDVLDSQLPISILIAMYSVVQIGGIIIMCIIYIPWFAISLPPFILFFLIIFDHYQSSAREVKRLEAVQRSFVFNNFNEVLSGLDTIKSYKLSDAFLKKNDFLTDKQNEASVMYMGLQKWSSVWIVLISVCLSTIIIFLCITRTFSIGAASTGLLLNYVLELSEALKGLLYETTQLENYMNSVERVVNYTTELPQEKAYYIAEMKPEDNWPQKAHITFENVSLAYRPGLPLVLKNFTIDVKGGEKIGICGRTGAGKSTIMSALYRLVELSTGRVVIDGVDISKIGLYDLRSKLSIIPQEAILFKGNIKRNLDPFDESTDEELWDALVKGGAIDANELNAVKQQKVFKGSKNNDMHKFHLYQNVDDEGANFSLGERQLLTLARALVRKSKILILDEATSSVDYETDAKIQAKIAEGFAQSTILCIAHRLKTIVKYDKILVLDKGEVKEYDTPLKLFATEGTIFREMCLRSGINETDFTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7J8</accession>
    <name>A0A1E5R7J8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Geranylgeranyl pyrophosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3271</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7J8"/>
    <dbReference type="FunCoup" id="A0A1E5R7J8">
        <property type="interactions" value="464"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7J8"/>
    <dbReference type="InParanoid" id="A0A1E5R7J8"/>
    <dbReference type="OrthoDB" id="6921389at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF44">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ82851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="357" mass="40790" checksum="496ADE0BCF25D8AB" modified="2017-01-18" version="1">MTEEKANELVRSTLSTISEVTKADNDYWSSKLENKIDEPCSYISRSKQGKQFRSLLIKTFNEIYYELDKPIIDMLSKVAERLHNASLVIDDIEDNSAMRRGMKTSHLVYGVAGSLNSANYSYFLALQELIDFEKNDSSQNGTNHARLAEVTQIFNEELLNLHRGQGLDIYWRDYRDKLDLSTIPSTTDYINMVKNKTGGLFRLMIRLMDCYSSFSVKETFKKERTDSILCLCDYLGIIYQIRDDYLNLLSDSAVASNKGDFAEDITEGKFSFPVVLGIHQELKDSTNGSRIYEILLKKTSDVVLKKEMLSLLEKFGIFSSTKKVIQELGNTVIKTYLHSRNIKTQSLQKVVESLTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7R9</accession>
    <name>A0A1E5R7R9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Styryl dye vacuolar localization protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3237</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7R9"/>
    <dbReference type="InParanoid" id="A0A1E5R7R9"/>
    <dbReference type="OrthoDB" id="3973193at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051402">
        <property type="entry name" value="KPR-Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708">
        <property type="entry name" value="PROBABLE 2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708:SF25">
        <property type="entry name" value="PROTEIN PAM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="380"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="603"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="723"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="819"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1002"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1099"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="485"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="380"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="603"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="723"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="739"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="823"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="859"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="875"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="914"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1002"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1039"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1197"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1214" mass="132078" checksum="38A2D6BD4DA06100" modified="2017-01-18" version="1">MSKSSSSTSTSKLSKIKKAVSSSINGEKMNSNKNTSENSENSENSENSEELHNNNNTITAKNSTGKPLKVLVIGNNPNVVLYCWRFAQAKTIDLSYVGNELIDQNVDIXFHPDGNSDGDVLHKFDTTSVNKYPNMEVFISTIQKTQSSSNTPFDIVIIGSDSLQDLSYVSSKLAYQLVISNATKIFVESSGFINLENFVSYSLPPAPLSPTSSNDQTNETKTSSMSAKVLSIITECDFRKIGENSFEKFGNTDTLYIGISSTASTETQYSKEASQIIKTLAKLFNKLFYNLTSFRVTTCSLNPHGFALKQWEIATPMIIFDSLLILFGIENPKDLNEQILTKPLISGLLLELQKIILKSTTITSLKAPSSSVXISTSVSSTSSTSSSSSSSPPPPQSTTTKSARLDDEEILMEKWLHQYSNKICPKLLYNFKTKRNSLNIDLLLLQPILIADDYNIKTPYLEFLYSMMIQFDKINYNNSTWFINKNDYQENLDVLQKKIYSLENRQHLELDQLLNQKELIQEQAKNLERENNKLKSQVENLSNENSSMKLQLKDQIENLHGQLNSVSLEKINLIKENKELNDSLNSFNQTNIVSKNSFAEKGSISNSNSNNDLPMGPQTSTPSRNASTPINGEFNGTVKSKRIQSGTDSELFVDARSTVENGMMSGDEEAMNEIKDFTSYGIFYGTPQKSVNVSSTEPSVVNXSETATAAAAAAAIATNTASTTXTASTTTTAATSNSGKLNTSTAATLNNGDDEDLSTDNHDATLDLDEEEIKDPHLRAIREKELELQKRELLLQQREKALSSGNVIQYGGPQFPVKQNMFPMPPPQQFPPMPRKPSLSNLQPGAPQHAGNVPGQAPEQRNLSGGKNSNNTNFPXQQQQQQMYIPQPQHHHHLQQQPHLNGLNHTATPPPSGQMPQPQRVSSAPQVYHPQYIKTSRKNRTSTAILPSASKLDITGAPPQMNTMHSAHQQGNASPMPMQXNGTFGNGKYGSLQQMSGKNMRQGSITTTSTSFNLNPSVLGPNSATPTNSGKHHYPVGPSSRNVSGNSTAVRNVSSSTELHSYGIANGAANGAANGAANGIANNLKNANGAFSQGIKGASVTSPGAHTDNAVDGQAPAPTGNTASQFTRVEIKPPSQPVVEDKAQEANVHNVPQSSXDAPQPPAIPHAADISTDSAXSGEEPSDTVGDLPVEQXDGKKAKKQKKKFSKFFGKSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R859</accession>
    <name>A0A1E5R859_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3458</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R859"/>
    <dbReference type="InParanoid" id="A0A1E5R859"/>
    <dbReference type="OrthoDB" id="4036231at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="485" mass="55069" checksum="836A4E1F9537D1CD" modified="2017-01-18" version="1">MRKFSGQRIQEGVDYIRVPSLQISSTELSHIPEAPSFSNFDLEDDDDDDNIQNWMNENVEGCYNSDDNGDDFDKVSLHHKKNLRISRTIKLQNRLSSIPVQLYKQDHDLWNNEQDTNSSLIPPGTKKNNNNKNISIRHSSVGTPLKSLQNITEEDDYLEETDTRATTRATTRATTRATTTMHLNMQQNIQLFPVVIDQQIPTTNNLNTHDDAFSNLYGEDEASILGLNQKCNSNVNQIQPTKSTKSIRSKNEILFDEILAGYMNKPFPKRKHWLSDCDPNVTSQINWQEQNSMNENKLSKESLVEEPAKVNPKIPNIKITLPPNLDEKIKMRLRSLTLSPTSPPLESVPMFEKTVSALQPPYVQRVKHQSFGTFDGSFEGESSENDMSSLGDKLYVSSPEAKTSLYFDSEVEEKDYGSPLKSLVMDQDTNQSTKKEPLPFVKHKLQKNFVKVPVPLIFDDSSEDELDDVFSFSSSVCTSVYSDTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R8A8</accession>
    <name>A0A1E5R8A8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Spindle pole component 29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3369</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R8A8"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="70"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="173"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ83140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="321" mass="36584" checksum="1B4703C64392A8C3" modified="2017-01-18" version="1">MVNFSFLGGNGNDKENTDEWSTNSFQMYKDNGEFTEDNLTNDPNVKQLQQQVIEANKKINDLLNTRHPLSXTNKQSSSNINDATNKHSGHLKYSPAYRHGSTRTVDHMNNSHDYNSNISNKQQDQMLLKQLRLGKPKEHVSESYGTTASANTNFHVPTPSSDSNSVSKLWNVVKNQQAEMARLNNELYMEKLRTEKMARDNKNMENEMYNVKTQIQMLTTQLESLHHSNNQYRTNENYQGANQYSTNGNYSAANKQSNNTTPTLLDDTQYLLSTSPSSFNSRKFADTKTQNGYSFTKQPSADIDSTTELIKGANKYINKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R935</accession>
    <name>A0A1E5R935_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein DML1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Protein dml1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3474</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the partitioning of the mitochondrial organelle and mitochondrial DNA (mtDNA) inheritance.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the misato family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R935"/>
    <dbReference type="FunCoup" id="A0A1E5R935">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R935"/>
    <dbReference type="InParanoid" id="A0A1E5R935"/>
    <dbReference type="OrthoDB" id="271881at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049942">
        <property type="entry name" value="DML1/Misato"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029209">
        <property type="entry name" value="DML1/Misato_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019605">
        <property type="entry name" value="Misato_II_tubulin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13391">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION REGULATOR MISATO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13391:SF0">
        <property type="entry name" value="PROTEIN MISATO HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10644">
        <property type="entry name" value="Misat_Tub_SegII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14881">
        <property type="entry name" value="Tubulin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Misato Segment II tubulin-like" evidence="7">
        <location>
            <begin position="4"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="DML1/Misato tubulin" evidence="8">
        <location>
            <begin position="208"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ83043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="538" mass="61367" checksum="97A7E687AE2D0966" modified="2017-01-18" version="1">MGNEIFNIGISHRSNHLLTQFYNLQESLLYAKTAGGLRKNEPSVYLSTKIDKINKLVDYTPRCLLWDAKNGNGSLGKYQYAATSTHDYFFHDNKINGKDQASWGDPGVSVEVQAHTRIPKSIFQNNLDQGIAQPSSEDLNGNTMDNFQYWSDYNKLIYHPQTLFSCQDWYHDEGSASGIPTNVSLSSNTALGKGKSEGPSDSSFITHFIHSQAGIEEYHKLSSDLWDSNLHYWLEQSDNLNHINIVSEFDTAWGALSNELLKSLKEEMPKISIFQFSLANIQPATSNGLLNIITNTLQCLENIDLLFPLYNEDSTKSLYDQTSQQAVLFETIASLFDNTELATKTVKQPHDLTNMLTLDSPERKIITNITSNVTQFDQVLTQEHGFFSKFALPTSFKPWKLKKIKSHVFHECEIYRVDNPEKVDKRDLSQDSVFTYDLDRSHINTFFTEPSKEILGLHSLNLKIQNDPMVNILKYWESVLSSPFIRKTMMQFPTSDIASPEDIDELVEKIEILKSTYLLKNQYSLYDSDDTSDEDDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA51</accession>
    <name>A0A1E5RA51_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mannosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2646</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyltransferase 22 family. PIGZ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA51">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA51"/>
    <dbReference type="InParanoid" id="A0A1E5RA51"/>
    <dbReference type="OrthoDB" id="3973071at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF3">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 11 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="227"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="309"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="359"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="12">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ83789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="525" mass="60807" checksum="8F481F5076F13F5A" modified="2017-01-18" version="1">MRNXTWTTNKEGLEMLRFGKNNKPWAMLAVVAALLLSVFSFSYIHPDEHFQSIEILLWKWNPNAFGVTIPWEFAQGARSFAPLFLWYLPLYPIISSYQPAYNPLHLLVVFRVYNYLMYVGILAFVLKKXYCNNKKQKYYNTFILVFTSHITISYQTHTFSNGIETNLLLLAMXLIHKLLKSQKXNRLFSLALGTILAVGIFNRVTFASFLFLPLLKLAMNSSIPKNIQILIPAGFMFLLVSYVQIKVDTYFFKSDSLVVSPIVNLTYNVNSANLSIHGLHPRFTHFLVNIPQMLGPLTIPLISWRVLKLDIWXLSIIGGLSILSIFPHQELRFLIPLMPIFCIVLVSQRLRIPKILFKAWFLFNITMYVVMGVLHQAGNIMFFQQKDLAKSVESSVHIWWKTYSPPTWMYLQPDMSSATTSFCLNKQGLQQIEVFENYKKGFNEVIDLKGCDTELLEQVLHHYLFGKNTSCIVNMPSSVAYKLKXMNYKFSKVWETKYNLDMDHFDFSNPESFKLGMAAYMVEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA61</accession>
    <name>A0A1E5RA61_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ribose-phosphate diphosphokinase</fullName>
            <ecNumber evidence="4">2.7.6.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g3002</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">5-phosphoribose 1-diphosphate synthase involved in nucleotide, histidine, and tryptophan biosynthesis. Active in heteromultimeric complexes with other 5-phosphoribose 1-diphosphate synthases (PRS2, PRS3, PRS4 and PRS5).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RA61"/>
    <dbReference type="FunCoup" id="A0A1E5RA61">
        <property type="interactions" value="535"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA61"/>
    <dbReference type="InParanoid" id="A0A1E5RA61"/>
    <dbReference type="OrthoDB" id="413572at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009156">
        <property type="term" value="P:ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000031">
        <property type="entry name" value="Probable PRS4-ribose-phosphate pyrophosphokinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000005">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000842">
        <property type="entry name" value="PRib_PP_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002320">
        <property type="entry name" value="PRK01259.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF32">
        <property type="entry name" value="RIBOSE-PHOSPHATE PYROPHOSPHOKINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00114">
        <property type="entry name" value="PRPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="13">
        <location>
            <begin position="6"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ83788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="319" mass="34662" checksum="339C47CA6C8A080C" modified="2017-01-18" version="1">MSSNSIKILAGNSHPALAEAICKKLGLPLAQINVYQYSNKETSVTIGESIRDEDVYIIQTGTGQDEINDFLMELLIMIHACRTASARRITAVIPCFPYARQDKKDKSRAPITAKLIANLLTTAGADHVITMDLHASQIQGFFKIPVDNLYAEPSVLNYIKNKTDFDNTILVSPDAGGAKRVASLADKLDLNFALIHKERQRANEVSRMVLVGDVAGKSCLLIDDMADTCGTLCKAAEVLLNEGNAKEVIAIVTHGIFSGNAFERLANSKLSRVVCTNTVPVNSNLAILDQIDISPTLAEAIRRLHNGESVSYLFTHAPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA77</accession>
    <name>A0A1E5RA77_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">VPS4-associated protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2834</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA77">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA77"/>
    <dbReference type="InParanoid" id="A0A1E5RA77"/>
    <dbReference type="OrthoDB" id="2158714at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013640">
        <property type="entry name" value="Vfa1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28218">
        <property type="entry name" value="VPS4-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28218:SF1">
        <property type="entry name" value="VPS4-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08432">
        <property type="entry name" value="Vfa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="206" mass="24183" checksum="27651595AF5E185F" modified="2017-01-18" version="1">MYNPNIYQGIADWFYCCDIHLEDNPSFMNPLYDTSYHTILEEAXTVDAKLQKTQEELKSCGSWDTWLNGFLNKKKTSSIEPEPALKTENKDQKNESSKEEGKSEETLKSKKEQXKQKXNKSAEETLKTKLSELAEQKKILTNKLFQIKQNTKKFEINDVMYRSKIMTIQQKMKKQKELKTQEEVYTRTDPTELMTKFPTLPKNNDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAE0</accession>
    <name>A0A1E5RAE0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein SBE22</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2561</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAE0"/>
    <dbReference type="FunCoup" id="A0A1E5RAE0">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RAE0"/>
    <dbReference type="OrthoDB" id="289721at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031403">
        <property type="entry name" value="Sbe2/Sbe22_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053949">
        <property type="entry name" value="SBE2/SBE22_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053948">
        <property type="entry name" value="SBE2/SBE22_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17076">
        <property type="entry name" value="SBE2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22874">
        <property type="entry name" value="SBE2_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22876">
        <property type="entry name" value="SBE2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SBE2/SBE22 N-terminal" evidence="9">
        <location>
            <begin position="303"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="SBE2/SBE22 middle" evidence="8">
        <location>
            <begin position="619"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="domain" description="Sbe2/Sbe22 C-terminal" evidence="7">
        <location>
            <begin position="776"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="302"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="586"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1114"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="81"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="169"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="321"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="334"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="350"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1114"/>
            <end position="1136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ83875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1164" mass="129918" checksum="0AD981C9B471C81C" modified="2017-01-18" version="1">MISKKDTFRISDYMDDHEHVTDDENDSVSPVDSQDSTEASGTINHNSGGAPIENEPSNAALGVSKNIQVTQMQPPFVASTKPLSNSSIGSSKTINGIRRPSDNLLSKMLLQETNNTTNSVINNVASTPTALQKNFNHGYIDSEDAMFNVPIALTSASILDVDSSQSNETTASSTRTSTATSTTTAKRYHQAGANYSSWPTSHESIVTNNTSISGDSNCEILDKKLKNNDLVLDTHATTTSSISTAETITTNSTNDKKDTFSGKPVLEAPVSRKMNTINSKPFIANADEVSNGNVTQVLEYAEQDGQTSSDEDGKDEGLTMRSVSSPLTAQNLYLRDKATGTRTYHRGESLSDSSQENETTSVEDPLHSTSTVNEVSNVSVDSISTIKGKQTPDIQRYMFNNGMQSTNSVLANTNDPLASSNGKFYSASSLPGNNNSSQNFFSSNSRPLTPNEMAMMQSYKMTPSQRLRMRREQNKNSVKNSIKYKEFYYDKHESIEKLRDQHGNIHSNQPSPLKTNTVNSAAFVQNNNISNMKNMKNKPASQSDDFLVWNVPVALNSVAFHQTPMMHSKAAKPSSSLQYSTTIGTKNASSTMGPQPSLGKRANSTSVIPSNPLSNIQVMHMAEMPASPIPGITAKGDKMNTLYDSTTKNLSQMYLSNSKSFSQVQLQTRMNSMDSLPQMVKTAGDEGLEDLKLVSDEKLKNVTSTRPMWLPIKSPREIKKHEFEISKTMSMVSIDKLNFNRKLDELPKKIADQKAKFDDIINKELQLSTNENSVNTINRNSLGQSLQNILWDCPNVITLEERLATYKKLTYFSLYENHFSSQLYNRFDDLYNNKYRTTEFNQTKLAEIDKLIKLNLVDYANVSEEDSTHNSIPENLITLIKLKMLTPAGLQNRDIIMFNILLKFYNADNGKACLETVWELNEVLSIVCFNDSVVEKFNEKIMSKSGPVYKYLSVESNFDQEFQTSNLKFDNFFHILDKLPESLTMWVLDVILVSNIIVFKSNNLEHYHKKLVTKLTEIKLTNPSSNSSSTNKYENMLFEYWNRKYMTKNYKVLVALVLNVLLNYHFGFNNFDELKKLSNKSYNLLGLPSSRSSSSLDNTSSSSVLKKHLSYRSSISSGGSRASMNSGPTSTTGTTGDKQHCIDGDEFVLIEGFLKKWLHYYKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAI4</accession>
    <name>A0A1E5RAI4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative abhydrolase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2575</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAI4"/>
    <dbReference type="FunCoup" id="A0A1E5RAI4">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAI4"/>
    <dbReference type="InParanoid" id="A0A1E5RAI4"/>
    <dbReference type="OrthoDB" id="8119704at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46118">
        <property type="entry name" value="PROTEIN ABHD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46118:SF4">
        <property type="entry name" value="PROTEIN ABHD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="35"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ83919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="356" mass="40452" checksum="C6132FD9BE9E66A5" modified="2017-01-18" version="1">MLKSFHRLLHHRATVKLNSIHFKPSNGLPLNHAQPIVVHVHGAFGSAVDFQYQSKKLANSFQTMVYSVDLRNHGESPRALPYDNWTLTNDLVSLIHTVSHQNGNKKVDLIGFSLGAKLALLAGFQKKAQTVINKIVSIDMPPGKLQEMDIIAVRNFKIVLDVLRAGAYYKQGKLSVQENKIHETNGKHFSKADLKSLQENLFLKTSGKIKDWMRPLENHFGKFNNYDPNLIAYFTAGFREGSLAPKVGEPLQHKIPLLKMPNYLNSICDWPTSVVESGNPLGIRPHVSQPTLFLRALQSPMFPLPDKSTQHHYDLIEKYYPNHRILDFDCIHPIHIAKPLEYYKAITDFLKVNQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAK7</accession>
    <name>A0A1E5RAK7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Glutamate synthase [NADH]</fullName>
            <ecNumber evidence="19">1.4.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">AWRI3579_g2963</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">Forms L-glutamate from L-glutamine and 2-oxoglutarate. Represents an alternative pathway to L-glutamate dehydrogenase for the biosynthesis of L-glutamate. Participates with glutamine synthetase in ammonia assimilation processes. The enzyme is specific for NADH, L-glutamine and 2-oxoglutarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>2 L-glutamate + NAD(+) = L-glutamine + 2-oxoglutarate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="1.4.1.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="24">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
        <text evidence="24">Binds 1 [3Fe-4S] cluster.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-glutamate biosynthesis via GLT pathway; L-glutamate from 2-oxoglutarate and L-glutamine (NAD(+) route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Energy metabolism; nitrogen metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Nitrogen metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the glutamate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="27">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.14" evidence="19"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAK7">
        <property type="interactions" value="653"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAK7"/>
    <dbReference type="InParanoid" id="A0A1E5RAK7"/>
    <dbReference type="OrthoDB" id="4327079at2759"/>
    <dbReference type="UniPathway" id="UPA00045"/>
    <dbReference type="UniPathway" id="UPA00634">
        <property type="reaction ID" value="UER00690"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016040">
        <property type="term" value="F:glutamate synthase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016639">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019676">
        <property type="term" value="P:ammonia assimilation cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097054">
        <property type="term" value="P:L-glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00982">
        <property type="entry name" value="gltB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00713">
        <property type="entry name" value="GltS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02808">
        <property type="entry name" value="GltS_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000207">
        <property type="entry name" value="Glutamate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000031">
        <property type="entry name" value="Glutamate synthase 1 [NADH]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000017">
        <property type="entry name" value="Glutamate synthase [NADH], amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000113">
        <property type="entry name" value="Glutamate synthase [NADH], amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000124">
        <property type="entry name" value="Glutamate synthase small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.60:FF:000001">
        <property type="entry name" value="Glutamate synthase, large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000001">
        <property type="entry name" value="Glutamate synthase, large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="entry name" value="Glutamate synthase, alpha subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028261">
        <property type="entry name" value="DPD_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002489">
        <property type="entry name" value="Glu_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006982">
        <property type="entry name" value="Glu_synth_centr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012220">
        <property type="entry name" value="Glu_synth_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002932">
        <property type="entry name" value="Glu_synthdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006005">
        <property type="entry name" value="Glut_synth_ssu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051394">
        <property type="entry name" value="Glutamate_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01317">
        <property type="entry name" value="GOGAT_sm_gam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008730">
        <property type="entry name" value="PRK11750.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43100">
        <property type="entry name" value="GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43100:SF1">
        <property type="entry name" value="GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14691">
        <property type="entry name" value="Fer4_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00310">
        <property type="entry name" value="GATase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04898">
        <property type="entry name" value="Glu_syn_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01645">
        <property type="entry name" value="Glu_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01493">
        <property type="entry name" value="GXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000187">
        <property type="entry name" value="GOGAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="Alpha subunit of glutamate synthase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18 24" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0274">FAD</keyword>
    <keyword evidence="9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0288">FMN</keyword>
    <keyword evidence="17" id="KW-0314">Glutamate biosynthesis</keyword>
    <keyword evidence="13" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="15" id="KW-0408">Iron</keyword>
    <keyword evidence="16 24" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="26">
        <location>
            <begin position="97"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="19"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="23">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1236"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1242"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1247"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00024383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00057049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00068518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR000187-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR000187-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="OEJ83938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="2194" mass="242426" checksum="B6AFF4D55A19C7DE" modified="2017-01-18" version="1">MSPPNAADHMQNGEYDMGINITSNTNTTTNTNTRHQSNRVVKSDKYDPMEAQDCYEGGKPMDLETQNFLKDNENKSWASTIPRPVGLYNPDFEVDSCGTGFVAHIKGHASHKIISDARPLLCNMAHRGATSTDGNGDGAGILVGMPHKFMVREFQLDCQVKVPQQYHYSVGNVFFKKNCDLSGSKEIFETVASKFNLKVLAWREVPRDSSILGSAALSREPHILQPLVVDADLDSDLDSTFDEVKFQTNLYLLRKQASKKIGLENWFYICSLSNKTIVYKGQLTPSQVYNYYYDLTNAHFESHMALVHSRFSTNTFPSWDRAQPLRLLAHNGEINTLRGNKNWMRSKEGVMHSDTFGEDLENLFPIVEEGGSDSAALDNVLELLTLNGLLSLPEAIMLCVPEATSLEMDSNLKAWYDWAACLMEPWDGPAALNFTDGRYCGAMLDRNGLRPCRYYITSDDRVICGSEVGVIPVANDLVIKKGKLIPGDILLVDTQVGELVDTEKLKKNFAQRKDFKSWLSKIIKMEDLLSKTEKLLPFENFHDYLLKNANEMGESLEKVQKDPRLLAMGYTFEQISLILVPMALNSKEALGSMGNDAPLACLNQDPVLLYDYFRQLFAQVTNPPIDSTRETNVMSLECSVGPQGNLLDMNSSQCDRLLLKSPILNWKEFESLKNIERAYPSWSIWQIDITFPKSKGLLGYTEIIEKITEEASQAIEQGKKMIMLSDRNLSADRVAVSSMVAVGAIHHHLVRNKQRSQVALVLETAEAREIHHFCCLLGYGVDAIFPYLAMETLVKMNHENLIRNANNDDITIDDEKLIENYQKAIDTGILKTMSKMGISTLPSYKGAQIFEALGVDNSVVDLCFAGTASRIKGVTFEYIAQDAFSLHERGYPTRTIVEPSINLXEAGEYHWRDGGFKHMNDPTAIASLQDSVRNKNEKAWDMYVKKEMEAIRGCTLRGLLDLDFKNSTAIPLDQVEPWTEIARRFCTGAMSYGSISLEAHTTLAVAMNRLGAKSNCGEGGEDPERSIVHPNGDTMRSAIKQVASGRFGVTSYYLSDAEEIQIKIAQGAKPAVGGHLPGHKVTEQIAKTRHSTAYVGLISPPPHHDIYSIEDLKQLIYDLKCSNPRATISVKLVSEVGVGIVAAGVAKAAAGNVNILISGHDGGTGAATWSDIKSTGLPWELGLAESHQTLVLNDLRGHTVVQTDGQLRTGFDIAVAILLGAESFTLATIPLIAMGCIMLRKCHLNTCAVGVATQDPELRAKFKGQPEHVINFFYYLINDLRKIMAKLGYRTVDEMIGHSEKLVKRETVNTKTINIDLNPILTPAHTIRPGVVTKFTKKLEDKLFARLDNKLIDEAEITLDKGLPVSIDASIINTDRALGSTLSYRVSKRFGPNGLPQDTVVVNIKGSAGQSFGAFLAPGITFILDGDANDYVGKGLSGGRIIIRPPPGSKFKSDENVIAGNTCFYGATSGYAFLSGCAGERFAVRNSGATMVVERIIGNNAFEYMTGGRAVVLSQLNSVNAFSGCTGGIIYCLTSDHDAFRSKINKDTIELSSLTDPVEIAFVKNLIKEHYNYTKSELAERILKNFNHYLTSFIKVLPTDYKKVLEKEAAAKKLEEAKATAAYLNKLEGGPCFENDATNGEVHALQNGQKKRALAVASHKASLKEPKVADLEDSIQDADQLEKNMEKLDKIKGFMKYKLRHEVYRPSKSRTSDWKELSGPISKKDAKYQTARCMDCGVPFCTSDTGCPISNVIPKFNELVFKNQWKLALDKLLETNNFPEFTGRVCPAPCQGACTLGINADAVGIKSIERVIIDNAFKEGWIVPKPPTFKTGRSVAVIGSGPAGLACADQLNRAGHSVTVYERNDRCGGLLMYGIPNMKLDKSIVQRRVDLLNAEGIEFITNCQIGKDVTMDELKERFDSVVYATGSTIPRDLRIPGRELKNIDFAMQLLETNTKALLNNDLEKVREQIKGKHVIVIGGGDTGNDCLGTSVRHEAASVLNFELLPQPPNERAKDNPWPQWPRIMRVDYGHAEVKEHYGRDPREYCILSKEFVGNDEGEVTAIKTVRVEWKKSQSGVWQMVEVPGSEEVYQADIVLLSMGFVGPEMIEDASVTKSKRGTIDTVHDASYSVDGGKLFACGDCRRGQSLIVWAIQEGRKCAKAIDSFLMGSTNLPGNGGIVKRDYKMLEELAASIPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAP6</accession>
    <name>A0A1E5RAP6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="8">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2756</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="8"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAP6">
        <property type="interactions" value="347"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAP6"/>
    <dbReference type="InParanoid" id="A0A1E5RAP6"/>
    <dbReference type="OrthoDB" id="3264224at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.20">
        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF20">
        <property type="entry name" value="[3-METHYL-2-OXOBUTANOATE DEHYDROGENASE [LIPOAMIDE]] KINASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase" evidence="10">
        <location>
            <begin position="329"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="25"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ83990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="468" mass="53480" checksum="CDDA7B36E6567868" modified="2017-01-18" version="1">MSRNYSTQTNNNVPXTNGNSSEVHHHQHHRHRRPTKYHWKPQDARTESQATLNDHLSNKFTYNTRFNIEKLIKXYANEPLLPISEIWKSYSSQYLIDNDDSVDADLGKNKKNIGKSTLSRKHLSSQQLYGLNLQTINLLMIYSCKRIKAFGELPYIAVINPKIEEVNKLYYQTLQDLLLMIDDATKFIDMGDHKRMIDALNKFIDNHNDTLIPLSVGLQEVVQDNWLMKECMSDFLNIHLRDRIVMKTLASHHLEILKQNVTAGNDNSATKATRDNSIGIISPELKISSLIQQVQEFVSDLCFVKYDQQVPIDIKYGHEITFPCIPTDLEYIMTELLKNSSRAHIESGKADVPIEITIVKTRGQTCKENDRKMFEELEIRIRDFGGGIPPLIEDKILDYSFSTVAKETKDGGMLDNQLPGEQVNTVSGMGFGLPLCKAYLDIYDGVLDVQSLYGYGTDVYIKFKSTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAQ8</accession>
    <name>A0A1E5RAQ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">uS12 prolyl 3,4-dihydroxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g2758</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[ribosomal protein uS12]-(3S)-3-hydroxy-L-proline + 2-oxoglutarate + O2 = [ribosomal protein uS12]-(3S)-3,4-dihydroxy-L-proline + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54160"/>
            <dbReference type="Rhea" id="RHEA-COMP:13817"/>
            <dbReference type="Rhea" id="RHEA-COMP:13818"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:85428"/>
            <dbReference type="ChEBI" id="CHEBI:138052"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[ribosomal protein uS12]-L-proline + 2-oxoglutarate + O2 = [ribosomal protein uS12]-(3S)-3-hydroxy-L-proline + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54156"/>
            <dbReference type="Rhea" id="RHEA-COMP:13816"/>
            <dbReference type="Rhea" id="RHEA-COMP:13818"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:50342"/>
            <dbReference type="ChEBI" id="CHEBI:85428"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TPA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAQ8">
        <property type="interactions" value="431"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAQ8"/>
    <dbReference type="InParanoid" id="A0A1E5RAQ8"/>
    <dbReference type="OrthoDB" id="430522at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031543">
        <property type="term" value="F:peptidyl-proline dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009896">
        <property type="term" value="P:positive regulation of catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006449">
        <property type="term" value="P:regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000014">
        <property type="entry name" value="Prolyl 3,4-dihydroxylase TPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.20">
        <property type="entry name" value="Oxoglutarate/iron-dependent oxygenase, C-terminal degradation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019601">
        <property type="entry name" value="Oxoglutarate/Fe-dep_Oase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043044">
        <property type="entry name" value="TPA1/Ofd1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039558">
        <property type="entry name" value="TPA1/OFD1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051842">
        <property type="entry name" value="uS12_prolyl_hydroxylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12117">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12117:SF0">
        <property type="entry name" value="PROLYL 3-HYDROXYLASE OGFOD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13661">
        <property type="entry name" value="2OG-FeII_Oxy_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10637">
        <property type="entry name" value="Ofd1_CTDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="13">
        <location>
            <begin position="138"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00081607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ83986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="634" mass="73280" checksum="AE4E84A5EBCBFB74" modified="2017-01-18" version="1">MAKRQAREEPVVTKRLNLEEDNIKKSFNDKIWQPEFRGALKEQIATAKPYNWGCIENLVTPELLMNVRKEIEHEIHFTNKETDIYKVNQSGDLANLSGLDWNDLGRLPNLFKLREVLYSEIYRDFISEVAQCGPLSGTKTDMSVNTYDKSCHLLIHDDVIGSRRVSFILYLPDPTKTWKEHYGGSLKLFDSIIPNIPCSDEYNKFIPQFNSIAFFKVQPGFSFHEVEEVKVDKHRLSIQGWYHIPQRGEKGFTPGEEEEWVKTNTSTLAQLENNTLKDYEFPKLIDREFLKNNALQXQAQVDDDFFTSEEVASLKEFISDDYLNPVKFKQLNQQFLETSKLEIGDFLNDSVVDTLKKRLRLYELNTECPYYYNDVQHPWKSAFPTHKWRYLYIDGKPDFRIAKGDDGKTLKKIERALPIDFNKVLKDLDNLSLNEQENAQTAAANNVQADEQVINDLVKIANFLKSSLFKKFINKLTSLKPISEKVLIRRFRPGKDYTLATTTPSSEDPQVLETPNYLENCLLEGTLCLTPSDEKSWNNGEVGGYQLYMLNQTDDDIEGQQDIEDAAVYKNDDTGDSVLINKTPSWNTLTLVLRDENVLEFIKYVSYNAKGSRWDVSMSWDVAGEDDEDNDEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAV2</accession>
    <name>A0A1E5RAV2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Bud site selection protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2592</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAV2">
        <property type="interactions" value="78"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAV2"/>
    <dbReference type="InParanoid" id="A0A1E5RAV2"/>
    <dbReference type="OrthoDB" id="783096at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005519">
        <property type="term" value="F:cytoskeletal regulatory protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1540">
        <property type="entry name" value="Actin interacting protein 3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022782">
        <property type="entry name" value="AIP3-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005613">
        <property type="entry name" value="AIP3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056279">
        <property type="entry name" value="Aip3p_Bud6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051825">
        <property type="entry name" value="SRCIN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22741:SF10">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN CG32809"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22741">
        <property type="entry name" value="P140CAP/SNIP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03915">
        <property type="entry name" value="AIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23153">
        <property type="entry name" value="Aip3p_Bud6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00806">
        <property type="entry name" value="AIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Actin interacting protein 3 C-terminal" evidence="4">
        <location>
            <begin position="374"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="145"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="197"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="616"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ84001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="841" mass="94998" checksum="57C831AA893992EE" modified="2017-01-18" version="1">MSSIQSRQASTQSNGMSSKEHGSSSSIESSVTKLIISTKNLLQKLTAWTRGEETQKTISDAYVQLGNDFQLVVKQFVHAGVDMQDMRNVPKELRGILEKALREKPSNEALDRYWPSIKDIIESLLTKIKTKRVILKQIENQKKNRASSNSLLSQISSTSSPSSSSSSSISSSFSVPSATVTQPQASLSPYNMLKTKQLPAQPESNPTNPVQTRRRSSSRILLSPEDLKPEATEPNFAEKTNNTAQSKATLAEDSSHKALAQLKKGDTIQRRASKRFSAYHIAKLTSTTSASATHPLPASMSQRETPVLESGHNEPELSSGKPTVFGVDMPKASKFEDNLTGDQQIKSPSSTFKPVAVFSDQRITSSEHRFINIFLKHQEXIKKITTTYPVLNKNTIIMLFAEKFSINYESDCLLYLRDPQYPHVSYDFDESEINTISDGFVIELVFSNAEKSESNEMIGNKQSEQNLAPTESTHETFQVLEQRLLASNAEMFKNLEKLISKNNIPCTDSSSNLAVTPLNFESKTCLAETNLSQETKLKTIEELKRDVLVLKQIQTENKTKIENSVASVVSQLAKLDPIKLLAAKDEAEDDAXLANGKKTSDRHYLEASQSKLSELSENLLTNVDDLQDTVEMLRKDVALRGSQPKKRKFDEVKKELDEALEQLQEMQKFLQVEKPFWKKVWEAELEKVCDEQQFINLQEDLYFDLQEDLKRCQETFHLVELCCEEQAKLKNVNKEKSKSDLDLSSKVILPLVKPGTMHEVREAVLNNIIELQPDHESRLEAIKKTEKHREKEKEMILNDEFNEELGNFVEKSQLNKSGGIEELERLRMEKDAENLRNMMSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAY9</accession>
    <name>A0A1E5RAY9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2662</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAY9"/>
    <dbReference type="InParanoid" id="A0A1E5RAY9"/>
    <dbReference type="OrthoDB" id="4489171at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="614" mass="70266" checksum="4299B9BFAB6C3918" modified="2017-01-18" version="1">MYTPTEQDLNTIKEVVNNNVDFSDAFLKELLVLYSGDINQVMEALLTGDYATSKTGELSKKTSDSDEEMGDTRETANDFDEKVSDNSKSVDDMTAPTININTKKSVQKSQLNKNIQYTTINMFDETDVAKHNSTNTPMFIVPKNCYNIGINYFNIFLYSIITNYTLPQDIVDXTLENSSHYPQNXFVKFIILYNAVKTQSNNKLFQDTQYYTVPSSLMDLDIEIPISETFSILIKSFINDYFILKKNIETKTXTTEDDNTEISASDPILNMLISTSSYYTTQQNVNNLYCMHFPQEEYTTNLNKMLNNLFLNQDNYESSQDKTNNVDLQVEQSNRLLKVSDVFTVIFDQEEYDDDDDDDDDDDMSLNNGNNLIDVENNLVDVDVPIIFYPELYTTPILKIIEKEIAPQLNSIKTQINELNVKLNMKLKYYQGAPVLSFLNTCLKYLSTEEDSDSGDNESVAVERRQNAIKTLNTVKQNLSSAKLTIIEQINVLKQQMHALDLNSLELSPTVVDLINKSQNANSDQSNIHMNVYKLVALIISSNNYFILNSKGKYTNYVVLNNTLNFNDTQVDDKMLHQLFKEYITTSKKEPIMCVYTKQENGEIAKKEDLIDMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAZ4</accession>
    <name>A0A1E5RAZ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 12">Ribonucleoside-diphosphate reductase</fullName>
            <ecNumber evidence="2 12">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g2861</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 12">Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + [thioredoxin]-disulfide + H2O = a ribonucleoside 5'-diphosphate + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the ribonucleoside diphosphate reductase large chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="2 12"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAZ4"/>
    <dbReference type="FunCoup" id="A0A1E5RAZ4">
        <property type="interactions" value="1281"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAZ4"/>
    <dbReference type="InParanoid" id="A0A1E5RAZ4"/>
    <dbReference type="OrthoDB" id="3000483at2759"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005971">
        <property type="term" value="C:ribonucleoside-diphosphate reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01679">
        <property type="entry name" value="RNR_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.70.20:FF:000001">
        <property type="entry name" value="Ribonucleoside-diphosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013346">
        <property type="entry name" value="NrdE_NrdA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013509">
        <property type="entry name" value="RNR_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008926">
        <property type="entry name" value="RNR_R1-su_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039718">
        <property type="entry name" value="Rrm1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02506">
        <property type="entry name" value="NrdE_NrdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573:SF6">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00317">
        <property type="entry name" value="Ribonuc_red_lgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48168">
        <property type="entry name" value="R1 subunit of ribonucleotide reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00089">
        <property type="entry name" value="RIBORED_LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0215">Deoxyribonucleotide synthesis</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-cone" evidence="14">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="802"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="808"/>
            <end position="834"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ84062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="920" mass="103010" checksum="2DE6148167376729" modified="2017-01-18" version="1">MHVYKRDGRKEPVQFDKITSRIARLCYGLDPNHIDPVSITQRIISGVYEGVTTIELDNLAAETCAYMTTIHPDYATLAARIAISNLHKQTKKQFSQVISDLYFYINEKNGLKSPMISDDVYQIVEKNKDVLNSAIVYDRDFQYNYFGFKTLERSYLLRINGQIAERPQHLIMRVAVGIHGNDIEKVLETYNLMSLRYFTHASPTLFNAGTPRPQMSSCFLVAMKDDSIDGIYDTLKECALISKTAGGIGLHIHNIRSTGSYIAGTNGTSNGLIPMIRVFNNTARYVDQGGNKRPGAFALYLEPWHSDIFDFIDIRKNHGKEEIRARDLFPALWVPDLFMQKVKENSDWTLFSPSEAPGLSDVYGKDFEKLYNKYVQEGRGRKTIKAQKLWYAILEAQTETGTPFIVYKDACNKKSNQKNLGTIKSSNLCCEIVEYSDNDETAVCNLASIALPAFINVSEDNQTQTYNFKKLHEVAKVITRNLNRVIDRNYYPVETARNSNMRHRPIALGVQGLADTFMLLRVPFDSEEAKQLNIQIFETLYHAALEASNEMAVEEGPYQSFQGSPASQGVLQFDMWNRAPSDLWEWESLREKIMKTGLRNSLLLAPMPTASTSQILGYNECFEPFTSNMYSRRTLSGEFQIVNPYLLRDLVDLGIWDDAMKSNIIANNGSIQHLPNIPQELKDLYKTVWELSQKTIIDMAADRSAFIDQSHSLNIHIMAPTMGKLTSMHFYGWSKGLKTGMYYLRTQAASAAIQFTIDQKVADQAAFSVADLSELKRPKYNPNAFKETGNFVARKETSFPRFVSEGDAPSTSSAAPAPSTSSAAPAPSTSSVAAESLPNEESTREATPISIANSNENTLVNSVESLSVSDKPVTQATKPAAVAKPAAPLNMENEDNWDIFNSKVIACAIDNPESCEMCSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB48</accession>
    <name>A0A1E5RB48_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Spp2/MOS2 G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2649</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPP2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RB48"/>
    <dbReference type="OrthoDB" id="4096940at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026822">
        <property type="entry name" value="Spp2/MOS2_G-patch"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12656">
        <property type="entry name" value="G-patch_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spp2/MOS2 G-patch" evidence="5">
        <location>
            <begin position="106"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="128"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="140"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="164"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ83793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="198" mass="22171" checksum="C34DDD0B199FFEC1" modified="2017-01-18" version="1">MSGTGFSLSLKKPKKQKKKPKSVLLEXETGVSFSQTTKLTKINLKRDQGTALGDAFDKRPKKKKASVVXINEIDSSTDYLQEKSKEQSKEQLCIQPAEVLINKDQTETTLDEYQHIPVGQFGMALLRGMGYEEEEEEEEENKKNEDAAQEKTRTGKKLATSGQTSTTFSSKKISSNSFAFSDQVFLPVVQKSRFPKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB70</accession>
    <name>A0A1E5RB70_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Eukaryotic translation initiation factor 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3070</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RB70"/>
    <dbReference type="FunCoup" id="A0A1E5RB70">
        <property type="interactions" value="1218"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB70"/>
    <dbReference type="InParanoid" id="A0A1E5RB70"/>
    <dbReference type="OrthoDB" id="10250831at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071074">
        <property type="term" value="F:eukaryotic initiation factor eIF2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005092">
        <property type="term" value="F:GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11561">
        <property type="entry name" value="W2_eIF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.350:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.170:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003307">
        <property type="entry name" value="W2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF7">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02020">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00515">
        <property type="entry name" value="eIF5C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51363">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 8" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="W2" evidence="7">
        <location>
            <begin position="241"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="389"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="393"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ84150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="408" mass="45449" checksum="5D9EA050147579FD" modified="2017-01-18" version="1">MSINICRSNHDPFYRYKMPPIQAKVEGKGNGIKTNILNASEVARALNRPVSYIIKYFGFELGAQTTIQENVDKYLVNGVHEPNKLQDVLDGFINKYVLCGSCSNPETEFVFNEKGNLKKDCKACGKITQVDMVHKLSSYILKNPPGGSDAGASGKKSKRKAATASSNVVGAGVSISDIAQQQQQEAEEGNSDDDDLARKINAEASKLETLEIEDDDWAVDMSEEAIRQRALQGEAAAAGPLSQDLVRLDQYGEWIVQQDEYPTDIELYKKAMDMELLEDPKLASILAQVLFEDEEIVTEIGEHAAFLIKVINGKPAFEKQFLGGLERWLGLQHPALIPLLPKILVSLYNNDIISEESIMHFGTTCSKKFVPKQTSKKVRRAAKPFITWLKTADDDEEEEEEEEDDDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBA6</accession>
    <name>A0A1E5RBA6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Thiamine pyrophosphokinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2656</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBA6">
        <property type="interactions" value="283"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBA6"/>
    <dbReference type="InParanoid" id="A0A1E5RBA6"/>
    <dbReference type="OrthoDB" id="25149at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030975">
        <property type="term" value="F:thiamine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004788">
        <property type="term" value="F:thiamine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006772">
        <property type="term" value="P:thiamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07995">
        <property type="entry name" value="TPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10240">
        <property type="entry name" value="Thiamin pyrophosphokinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.320">
        <property type="entry name" value="Thiamin pyrophosphokinase, thiamin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006282">
        <property type="entry name" value="Thi_PPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007373">
        <property type="entry name" value="Thiamin_PyroPKinase_B1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036371">
        <property type="entry name" value="TPK_B1-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007371">
        <property type="entry name" value="TPK_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036759">
        <property type="entry name" value="TPK_catalytic_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622:SF8">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04265">
        <property type="entry name" value="TPK_B1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04263">
        <property type="entry name" value="TPK_catalytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00983">
        <property type="entry name" value="TPK_B1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63999">
        <property type="entry name" value="Thiamin pyrophosphokinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63862">
        <property type="entry name" value="Thiamin pyrophosphokinase, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiamin pyrophosphokinase thiamin-binding" evidence="6">
        <location>
            <begin position="311"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="85"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="103"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ84161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="407" mass="45906" checksum="A7DD2C1F64166A06" modified="2017-01-18" version="1">MVHLDRSGKVQSTIPKPVKSKNLSETXTVLPENCFAGTMLDEELLVKVTEHAERVTLELPQDYQAQLDIREIFTPQKTLLINGTAHSHKQHNNDENKENVDGNSSSTNNNTNSISSPSNHTAXPQHEPYGLIILNQDIDILPELFRKLWEKCHVRICADGGGNRLYEFDRSYVPDYIIGDFDSLRPEVAQHYASMGTVVIRQKTQLSSDLSKALSVMKLTFLNLLKDNYIDTTKENNGIEIFDGIHKLDEKHEADFLEKVPNFKVICLNGIGGRFDQTVQSIAHLYYDWLNVPSPTSNCGTCDPKMIFVTGSDIIIKVPKGPFYLKYDAEIKDKVLLNCGLLPLGNPTTVVKTVGFKWDVVDWETSVPKGRVSSSNRFSNTNGCYINVKEDLILNIEMNYEQLLHII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBB3</accession>
    <name>A0A1E5RBB3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Signal peptidase complex catalytic subunit SEC11</fullName>
            <ecNumber evidence="4 15">3.4.21.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g2482</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalytic component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Specifically cleaves N-terminal signal peptides that contain a hydrophobic alpha-helix (h-region) shorter than 18-20 amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.</text>
            <dbReference type="EC" id="3.4.21.89"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the signal peptidase complex (SPC) composed of a catalytic subunit SEC11 and three accessory subunits SPC1, SPC2 and SPC3. The complex induces a local thinning of the ER membrane which is used to measure the length of the signal peptide (SP) h-region of protein substrates. This ensures the selectivity of the complex towards h-regions shorter than 18-20 amino acids. SPC associates with the translocon complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the peptidase S26B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.89" evidence="4 15"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBB3"/>
    <dbReference type="FunCoup" id="A0A1E5RBB3">
        <property type="interactions" value="663"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBB3"/>
    <dbReference type="InParanoid" id="A0A1E5RBB3"/>
    <dbReference type="OrthoDB" id="10257561at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009003">
        <property type="term" value="F:signal peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001733">
        <property type="entry name" value="Peptidase_S26B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02228">
        <property type="entry name" value="sigpep_I_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806:SF6">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00728">
        <property type="entry name" value="SIGNALPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 15" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ84188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="182" mass="20712" checksum="E524EA2211FD9846" modified="2017-01-18" version="1">MNLRRELTNVLNLCFIFTGAFMFWKALGIVANTSSPIVVVLSGSMEPAFYRGDVLFLWNRNQYNNVGDIVVYEIENKQIPIVHRVINEYKQVSSKNSKSKKKDKSQDLDTTQYLLTKGDNNNGHDVPLYGHGKQYLQKDKDIVGTVKGYLPQVGYITIWISENKNNQLIFYGVLGLAALLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBD2</accession>
    <name>A0A1E5RBD2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transcriptional activator protein DAL81</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2479</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBD2"/>
    <dbReference type="InParanoid" id="A0A1E5RBD2"/>
    <dbReference type="OrthoDB" id="2264294at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001080">
        <property type="term" value="P:nitrogen catabolite activation of transcription from RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050797">
        <property type="entry name" value="Carb_Metab_Trans_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668">
        <property type="entry name" value="GLUCOSE TRANSPORT TRANSCRIPTION REGULATOR RGT1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668:SF4">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN DAL81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="484"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="107"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="172"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="293"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="362"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="616"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="26"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="107"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="122"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="135"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="192"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="207"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="263"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="297"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="309"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="333"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="362"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="417"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="444"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="460"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="9">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="9">
        <location>
            <position position="1173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ84210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1278" mass="142775" checksum="21118E85945784AE" modified="2017-01-18" version="1">MSFKSPSNMSIDPSVEQNSSTQKSVDMLDKADDTSRPENLKGSTENDTSNTGGPHGKIDEHNNNTDXTASLNIDKQQSLFANNKEDQNVNNSIDSKLASDNHSAIQNKGQGEANISXNGNEGTERAVSEDRISDKNAQGSNTEKSGSSNVNTTMLEFGDDYTNMLSNLHYGSHDYKHQKNPNFDTSATKNESNTDHNTNADHLRGNFNEQTIATKNNNEGVQHGDGENDLKDRKKSNDESSKNENLDMLIQHYEGLIKKHNDQATNGNDHQNQSWNTHSNDDERKLKTFENQKEKNNAQPDAQSSEFSKNNESKGLYKSDQKDGISKVHTTDSQRSTPMPMQQNSSHSLHTNLQNVDAYTSXTANTKGSPERNTPTSNNLFNTTQVQSPNSLFSSASPLVTNPVESDIKYGTPKDASSANNDNSGXRNSSSKPIKGGKKPKKANTPTSAKAKLNNTKQPNKSSTSSNASSNNGHSTVINQDRKICDHCRRRQTQCVQVPYLQNCIQCESKGIKCTYSELAATKNSSEIEQLKRSRVDENVNVHDLLKRAKLDPSMTNGNGNNTQNGGTNVYQDPNANNVNSLQSYSEMLHKIGPQQFNYNANLVSAISGMQNTLNSQEASGNQSLHPNSNSGTFSYIPKTSNMASPNDKSFADNSNSNNPNFYQQQLQFQLQQQQLHNQSMFPHGSPHIQSPASLPPHMIPQQQQMPLPQIHDLQQMTANALIPGVNGPGSYFNRSNGPSNVAPSYPRSSFYVGPTSLFDYNLINHVKLDKIDQIQISPSLTLRKVAPNVQFVLRDDFNEKLYLKQEQEIDLVEKLIHPHGKMLVDIFFKVIHPYYPILHERVFMEKYSRSYRELTAPLLASIYSLALQWWDHHPQLVGFMKPDVTEQLNELALKTFFDVVERPKLSIVQAGLLILMCRSESSNNWVIISEVVALAEELGLGIDCQDWRLPRWERGLRRRLAWAVWSMDKWISASEGRYSHLILGRNWLVKMLSDEDFPEDPNNILSGNSISGSSSPAANFVPYAKNSKTPNSSTNLAESMQANNLTNFMESHTPAQGPGSPGNFIMDIQPTPNDIRTGRQLFQQFVSLSIILGEILDTFYTLGAMSITTKVDYVLKLAKPLQLKLREWYHSLPAQLSMNYLQPRRLNSNASLTLAYFAAEITLHRKIISTLDASTPADLTHVCRQAAKTRLSAAIDFICDLKXEHTNSFWYSCSSNNMALIGSFAALLYCTSSSANEKELYRNYLRKYIITLRISAKNFEKTRAALEFSQLLDEILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBH9</accession>
    <name>A0A1E5RBH9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Spore wall maturation protein DIT1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2640</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBH9">
        <property type="interactions" value="255"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBH9"/>
    <dbReference type="InParanoid" id="A0A1E5RBH9"/>
    <dbReference type="OrthoDB" id="429813at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007817">
        <property type="entry name" value="Isocyanide_synthase_DIT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37285">
        <property type="entry name" value="SPORE WALL MATURATION PROTEIN DIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37285:SF5">
        <property type="entry name" value="SPORE WALL MATURATION PROTEIN DIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05141">
        <property type="entry name" value="DIT1_PvcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ84254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="607" mass="69208" checksum="BC63575E1AE14B22" modified="2017-01-18" version="1">MSESGAQIANDYKISHGPSAGRMSTYSKFLCIYTRNAKSFALNSIRDKQSSQFGKHWTEFVSQLKEANFTTTKHETRECRISCSQVEKYTNLPGIDVKITELQKIGESQIRGIITSVESENDFGNWFVNHMLHLSRLDMTETASKDPVNSIDPAAHKKLADFFAENLKNTVKHDKWLEGGRNHFVKRLQYFTSRNIKVECILPAFPCKSSNLEKVGGVSPDMGEFMALHRLVQAAKDVAQFYPPGIKFWIVSDGHVFSDCIGVDDDVVDEYTEHLHKQYQTIKETSDKEEHVGFFGLKDIFYSGKFGKQYDPKWNESLILPHFTGTRIQKDSETCRKIMMRGCDTDDGRLEEQVKIPNHPRLLLYRGFAKFMEEDLKRLPYFANHSRKQFKKTASRVAFEMIKRNDAYSNLVDLIFPHHMRLSIHAHPNSGPKFGIKVISDEKCRMVKSLENNEKPIFEDFLHIPTPWHNTVVYISDDDQYYLTRSNVVHSAIKKGYYAGEWVPNNGAGYFHISKVSYAIPTVDGSASSIEQESSMDLSSDSLRSDVFSQASRNDSSFTPITSNLSTGGKPSFASINRMDKTQKDRNVEELVQDLKKXFIGXGNKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RCK1</accession>
    <name>A0A1E5RCK1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">General negative regulator of transcription subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g2808</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the CNOT2/3/5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RCK1">
        <property type="interactions" value="169"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RCK1"/>
    <dbReference type="InParanoid" id="A0A1E5RCK1"/>
    <dbReference type="OrthoDB" id="293823at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1020">
        <property type="entry name" value="CCR4-NOT complex subunit 2/3/5, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038635">
        <property type="entry name" value="CCR4-NOT_su2/3/5_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012270">
        <property type="entry name" value="CCR4-NOT_su3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040168">
        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007282">
        <property type="entry name" value="NOT2/3/5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007207">
        <property type="entry name" value="Not_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04153">
        <property type="entry name" value="NOT2_3_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04065">
        <property type="entry name" value="Not3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005290">
        <property type="entry name" value="NOT_su_3_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0678">Repressor</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CCR4-Not complex component Not N-terminal" evidence="13">
        <location>
            <begin position="2"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="NOT2/NOT3/NOT5 C-terminal" evidence="14">
        <location>
            <begin position="460"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="222"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="300"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="586"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="128"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="222"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="241"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="300"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="331"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="359"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="586"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ84313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="659" mass="76324" checksum="96B32D7761ACEE42" modified="2017-01-18" version="1">MSQRKLNQEIDKLLKKVKDGLQDFDQVYDNFQESEPSQQSYREKLEGDLKGIIKKLQKQREQIKSWLSKDDVKDKEQSLLDNRKLIENGMERFKSVEKLMKLKQFSTQALANPNIVMNPKEIKKLETTTFIEESIEELRKQLETLEANEDHGKIERHTFHIANLENILRLINRDDLDCDIVEEYKDDISYYVENNLEDPDFVEYEDIYADIGCEIQPNDFENTDISFNGMDSTPKKEKHNINNNNNNNNNNNNNNNNNITSNNHSTSNVSLLNGKSKKHTAKISSKLEKLNGNVSSVIDSSSAISSAVPSRSPTPSIPMERQASSKDGQKNVLSSATLDSKLSSKEKNGNGKLQNGELEENKKLSNIIDKKQKDTDTSKEQILKKDGEKNETRDLLXEIEQQKQQRLKEIDEILTEDLQKPAFNNPLFSPGLKDWLSTKKSLMAPSDNMTEEMSKQLEFSLLNCPDSLDSEVPKLHQFDLSQPHPTSIFFPQEPIKFYYLPHFSNNTVGNVERLHANWRKNDLYSRTSLAQIFTKFDLDTLFFIFYHYQGTYEQFIASRELALRKWLFNKMDRFWYQKQREPVFPSSANASRTNLDSAESLNASNGNAQTNTKEEKQEGDVLGEEETVESWRYFDYKSSWITRRVGSDFVYNSDHFENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDL2</accession>
    <name>A0A1E5RDL2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">60S acidic ribosomal protein P0</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2131</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. uL10 forms part of the P stalk that participates in recruiting G proteins to the ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDL2"/>
    <dbReference type="FunCoup" id="A0A1E5RDL2">
        <property type="interactions" value="1523"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDL2"/>
    <dbReference type="InParanoid" id="A0A1E5RDL2"/>
    <dbReference type="OrthoDB" id="10259902at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05795">
        <property type="entry name" value="Ribosomal_P0_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1730:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000001">
        <property type="entry name" value="60S acidic ribosomal protein P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050323">
        <property type="entry name" value="Ribosomal_protein_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030670">
        <property type="entry name" value="uL10_eukaryotes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039087">
        <property type="entry name" value="L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="7">
        <location>
            <begin position="109"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="278"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="278"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="290"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR039087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ84990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="313" mass="33849" checksum="367EE19747F965A5" modified="2017-01-18" version="1">MGGVREKKAEYFSKLRTLLEDYKSVFVVGVDNVSSQQMHEVRKVLRDRAVILMGKNTMVRRAIRGFISDLPDYEKLLPFIKGNVGFVFTNESLKDIKDVIIENKVAAPARAGAVAPEDIWITAMNTGMEPGKTSFFQALGVPTKIARGTIEIVSDVKVVAANSKVGASEAALLNLLNISPFTYGLSIVQVYDNGQVFPSNILDISDDELVSHFVSAISTITAISLASGYPTLPAVGHQLVNNYKDLLAVAVAAKYVYPEVEELFDRLENPEKYASAAPVAAAASSGSADAAEEEEAEEEADESDDDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDQ6</accession>
    <name>A0A1E5RDQ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein SDS23</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1784</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SDS23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDQ6">
        <property type="interactions" value="253"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDQ6"/>
    <dbReference type="InParanoid" id="A0A1E5RDQ6"/>
    <dbReference type="OrthoDB" id="3971901at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004865">
        <property type="term" value="F:protein serine/threonine phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000920">
        <property type="term" value="P:septum digestion after cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000035">
        <property type="entry name" value="Protein SDS23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000043">
        <property type="entry name" value="Sds23p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF36">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0129">CBS domain</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="8">
        <location>
            <begin position="306"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="8">
        <location>
            <begin position="403"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="77"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="522"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="8"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="522"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="546"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="572"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="616"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="719" mass="78203" checksum="4175D79B542A63EA" modified="2017-01-18" version="1">MPHINQPNSNASIHSNSSSRXTSSTSLNQLVSNNNNNNNNXNNGNNSGNNGNNGNGTNSGSTSQLHALMPTRHDSIVKMLSTPPPDNNNNNTNEGSNXTNEGSSATNEGSNITXGESDTAHFVXGSYDTNMIDSXDANIGNVNNDNSNNTLSPLEKLHISKESHHMMRPSLSNSSISSNSSLEDTAFNQLVNLTEEQKKKEKLVLKVHKKSWQHIKLSSLIEPNKLITINGQENSVEMAFNKLIEYNLTSIPVENFPGDMNCLTFDYNDLNSYLLLVLNKIKVNDMQIMKDCQSGKPVAVGEIIKLTPKNPFYKLPECENLSAAMSILGTGVHRIAITDLKQSQIKGILSQRRLIKYLWENARKFDNFENLLNMSLKDLKIGVFGLETQHYKRKINPITGTTIHQSRVISINGDEPLINALYKMHEESISSIAVIDNSTMLLGNISVTDVKHVTRTSQFPLLHNTCRHFISVILNSRGLEMGGKDSFPIFHVYPNSSLARTMAKIVATKSHRLWIVEPPNNQFQSSPIQAPSASGNIHFPTHQHRNSLSSEDLSASATTSDFSNHHLNDNYNNAGTSASASASASANTSAQSHHRNRSNAGMRDSPLDSPLAKVPSNGSTDSPLTQSPLTQSPLTQSPLSTSINTISTGTPVVPTSSNNXPMTTSASAGEGLGIGAGKLIGVVSLSDILGLFVRNELNSLVDPQTARRQRSIASSHERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDV3</accession>
    <name>A0A1E5RDV3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pyridoxal phosphate homeostasis protein</fullName>
            <shortName evidence="2">PLP homeostasis protein</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2144</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Pyridoxal 5'-phosphate (PLP)-binding protein, which may be involved in intracellular homeostatic regulation of pyridoxal 5'-phosphate (PLP), the active form of vitamin B6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the pyridoxal phosphate-binding protein YggS/PROSC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDV3">
        <property type="interactions" value="496"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDV3"/>
    <dbReference type="InParanoid" id="A0A1E5RDV3"/>
    <dbReference type="OrthoDB" id="10264196at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06822">
        <property type="entry name" value="PLPDE_III_YBL036c_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02087">
        <property type="entry name" value="PLP_homeostasis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011078">
        <property type="entry name" value="PyrdxlP_homeostasis"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00044">
        <property type="entry name" value="YggS family pyridoxal phosphate-dependent enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10146">
        <property type="entry name" value="PROLINE SYNTHETASE CO-TRANSCRIBED BACTERIAL HOMOLOG PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10146:SF14">
        <property type="entry name" value="PYRIDOXAL PHOSPHATE HOMEOSTASIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01211">
        <property type="entry name" value="UPF0001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alanine racemase N-terminal" evidence="4">
        <location>
            <begin position="104"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="318" mass="36024" checksum="2015CA3B47D3A6EB" modified="2017-01-18" version="1">MFLRSRLSPLNIDRNISIFSLANSLNGLKVSTTTPTPQTALQTRQKSQMTDVSLNYTDARCNELYENYTTTLKQLEKECSINSRSSKDVLLLCVSKLKPASDIQALYDKGIRHFGENYVQELISKXEVLPKDIKWHFIGGLQTNKCKDLANRVENLYCVETIDSLKKLKKLNEQRCKYVEGLGQESDAVPNGKHTINVNIQINTSGEEQKSGLSNESEFFQMIDFFKSSECKYVKLNGLMTIGSWDVSHSETEENKDFAALVSWKSKLTDYYREKYQTDVASVKLSMGMSSDYIQAIKQGSNEIRVGTSIFGSRPVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE31</accession>
    <name>A0A1E5RE31_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ran GTPase-activating protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2087</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RE31"/>
    <dbReference type="FunCoup" id="A0A1E5RE31">
        <property type="interactions" value="168"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE31"/>
    <dbReference type="InParanoid" id="A0A1E5RE31"/>
    <dbReference type="OrthoDB" id="184583at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027038">
        <property type="entry name" value="RanGap"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113:SF12">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="354"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="377"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="410" mass="46220" checksum="3201E3905C49CF09" modified="2017-01-18" version="1">MATLHFKPEHKSSDVFDISGKALKLTSGEDIEPILKDLQKLPTVTKIDFNGNTIGVEASKKLSECIAQTPAIYENLQEVNFADLYTSRLVDEVVDSLNYLLPALLKCSNLYIVNLSDNAFGLRTIDILEKFISNAVQLQHLILSNNGMGPFAGERIGKALYVLAENKKKNKKPLLETFICGRNRLENGSTKFLSLGLKNHAEGLKSIKLYQNGIRPTGIITLLKYGLQYNTALEILDLQDNTLTSSAALVLCEYLQKWNKTLKELNLNDCLLKTKGSDALLKKFQELTFENLEVLKLQYNELSHESMVNHLIPALSKKHLPNLKFLELNGNRLEEDSDDLETLIEAFDGELDELDDFEEIDSDEEDEEEEEEEEEGNAEKLEELDSAALEKDLKEQEVDKLSSLLETTHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE69</accession>
    <name>A0A1E5RE69_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2341</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RE69"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="482"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="537"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="417"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="417"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="3">
        <location>
            <position position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="566" mass="63808" checksum="CB8366788FAA747C" modified="2017-01-18" version="1">MSYSHLHHHRRDDDKEKEEGPDTQWQYHYQRQFSPDLPAFPSRTLPHSVNDSPNTTGSPNIFGASTIPSSRTNPSTLDSAQGMEMFKEAEIAYRYSFNPQKQEQIEQQQRQKYQEYTNYCPPPELRPVQSFTAEELAQNPTRTLQNKGHPLSNTIAQTGYGTRSGSFLSASSGNGEYFEKTHGTRSKETLQKMASSYLSETPGPTENQKTMSKHMSSFLKPGDELAIPTVDPVRSLMHRNSQMTENVSSASNNETVNTSILFSSSSSNTNNRNSMPLSFHSNQLNILQPDSARVSYNNAIAESLHNTTTNNNXDPRFFNYNTTAALPPSDNDAESRGYYANTNASIMMQKYAQQPKPPFSQSGLSFPHDRVFSNQSTSSASQEMLTRSIQLNRKTKKTNHDYHGKKEIMGEHRILTPSSQSNLNSHENTQSEGLYSEEVKNEPIWQQNTGLRKTSLPESNFYGETFSPGTVPNVSVINTRTAXLFCLSCVIPCLFVYFSFLLSDEKLLALMYHTDSLAYAYIQRYENHKLKLHKLKIFXLISLGIFAVESCVVILAIVLLLVLARN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REK0</accession>
    <name>A0A1E5REK0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ankyrin repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1718</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REK0"/>
    <dbReference type="FunCoup" id="A0A1E5REK0">
        <property type="interactions" value="176"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REK0"/>
    <dbReference type="InParanoid" id="A0A1E5REK0"/>
    <dbReference type="OrthoDB" id="19174at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="31"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="205"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="219" mass="24789" checksum="EA43B8C9CD0DBAC4" modified="2017-01-18" version="1">MNIWIAASDGRQDLVEKYLASGLSAVAKDPNGYTPMHAAAAYSHLQLLEFLVNKHNGDINVRDEDGDTPLHHVEDVATAKFVIEQLHADPNLRNNEHLTALDNVLQDSDSDPQLIEYLKSVTLKDGQHGNIQEQLTGLINEDELNQFKENVKLSLATEVPQDEESLQRQKRLQEIMNGDNVDENLEQYITEIIHEQLLNQVNPKRGHEDQADSNKRSRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REX2</accession>
    <name>A0A1E5REX2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein FMP25, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1966</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REX2">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REX2"/>
    <dbReference type="InParanoid" id="A0A1E5REX2"/>
    <dbReference type="OrthoDB" id="10256179at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053245">
        <property type="entry name" value="MitoProcess-Associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47563">
        <property type="entry name" value="PROTEIN FMP25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47563:SF1">
        <property type="entry name" value="PROTEIN FMP25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="423"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="593" mass="67670" checksum="2F36438284BEF2C6" modified="2017-01-18" version="1">MLKSYRAFPALRTGLKVRSTLKVPAGVVGFQLRFNSGNSLKDKIEREQSVDASELFSDRITSKTYYAKREHPKNEQVKQILKERQQAWDNHELAQTEGIKILSEQEQRDRMEKMTRLNSLFQGGLFLLVALGLAFYFTSDNAFFLNKNSSLIDEEKMEQKLNMNNAKKDKKLKKISAQLKAETSTCQMLISNKSNNELVQWKNGKAGTLTALAVGNGKKDEVLDVVECGDDLLYVNKRGDLYKLNDNTESXKGLQKDTLVLKDQHLTQLKATSNNELIAKNSKDEVLVIPLTSSKYKEYVSSKRSKLLPWKHYDEYSKKLPIPFKTYDLGESHFVGIGAKDNLVYTCSLVSSEKVKHKGQFGLPKYAPIKIHDNEDLARDLNPFVLENVELLNYLIKNDCLQERQFVKVSCGKYHTVAMDTNGDIYTFGNNTFGQLGVNVFYDTENISYPKQLRKLPQLSRDKSYDWKCKDIKCGDYTTYLKVKDENDSKYTYFSFGSGMKGELGNSHFKSSQQSPTKMKYDLQDCIKEWEFQGSQCALVTSKNELYVWGSNNGNSLPGILPNKNYAYPVDTGFKLQPKTRVLITKNHGTIIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REX3</accession>
    <name>A0A1E5REX3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1970</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5REX3"/>
    <dbReference type="OrthoDB" id="60822at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011893">
        <property type="entry name" value="Selenoprotein_Rdx-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02174">
        <property type="entry name" value="CXXU_selWTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36417">
        <property type="entry name" value="SELENOPROTEIN DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G05220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36417:SF2">
        <property type="entry name" value="SELENOPROTEIN DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G05220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10262">
        <property type="entry name" value="Rdx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="104"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="168"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="132"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="168"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="194" mass="21740" checksum="ABFB7D740E71FDE1" modified="2017-01-18" version="1">MSESVKVSIEFCNKCKWGLRSFWYLQELFQTFEDKIAELSVKPNNNNPGTFKVVGYITHTNGTESSIQEVTIWDRRVDGGFPDAKTLKKRIKMLLFHENNDVNIGKHNERGPSTLPNERGPSTLPNERGPSTLPNQRESSTLQNHTVATEKQDGVLISDTNVVTSFXPSNLTSDAQQPKTKECAECSECQVAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF00</accession>
    <name>A0A1E5RF00_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glutamate--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="3">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glutamyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g2258</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RF00">
        <property type="interactions" value="711"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF00"/>
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    <dbReference type="OrthoDB" id="428822at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00808">
        <property type="entry name" value="GluRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000045">
        <property type="entry name" value="Glutamate--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00022">
        <property type="entry name" value="Glu_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004527">
        <property type="entry name" value="Glu-tRNA-ligase_bac/mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049940">
        <property type="entry name" value="GluQ/Sye"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033910">
        <property type="entry name" value="GluRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00464">
        <property type="entry name" value="gltX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43311">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43311:SF2">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="An anticodon-binding domain of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="12">
        <location>
            <begin position="41"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class I anticodon-binding" evidence="13">
        <location>
            <begin position="399"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00072917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF19269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ85465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="528" mass="60062" checksum="6FA93F2534BD29D7" modified="2017-01-18" version="1">MLIIRRAYSTGKLRSSLLGSKSKELLKKKPVQNLHPQKPARTRFAPSPTGFLHMGSLRTALYNYLLAKNTGGQFLLRLEDTDQKRLVXGAEQNIYDSLKWLSIEYDEGPGKNDNTDMGPYRQSDRSKIYSEHVETLLKNGHAYRCFCSKERLDGLRDSAQKLQPPTTVSYDRHCLKLSKSEIAQKLEDSESFTIRLKSPDQYPEFEDILHGKLNIQPQINYNDVRYDDPILMKSDXLPTYHLANVVDDHIMKITHVIRGEEWLPSTPKHRALYNAFGWESPKFIHIPLLTSANDKKLSKRKNDASVLAMKEKGVLPEALLNFSALFGWSPPRNISAXTHDCYSLKDLIKIFDLNNLTKGNAKVDEQKLWFLNKHHLHKRLEDPSTFNECVDEIHAXMGQXYAKDKIAKVLEKVGPSLTTLKEFPVSYSYFFEKPDYKTSGVLNFKENHEVSEXKKVLSSLDGISPQNATQMXDDLAKSLGVPKKLIFESLRYALAGSHSGVKLPVLLDLLGPEETQXRISEGLMSLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF37</accession>
    <name>A0A1E5RF37_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2407</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RF37"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="498"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="152"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="502"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="534"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="583"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="600"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="720" mass="82314" checksum="F1D38C672C240C15" modified="2017-01-18" version="1">MVKEELKEQQNKNESVAEREDVKEVSVPDSTGIELDIEAQVEEEEIEEEKDIKHTTPSTPIKNQSSVQEFTIEEDEDEDENEDEDDEKEDSGNSNNTADTHREEDQYFEANQSVSDIHSTSSPLLKSGDQNNKLLHTNTTSNGLSKNIKLLSESSDGSSSSENEPTGKLKENAENNYSVTIEDEDGKVIAYHEQPASPNTQEQAKSDQNDQXXEEEEEEEEEEEEEKEEHDADDDXDDDEKNKQEQEASKEMARYNDNEEYPLYHEEIIGDINQYKIYKKTSRIEITAYLNTKIQDFCAEHEMQLITGNAQFPDEELATALNYMRKIGPFKGAELIINSEGNIKDQEQQELSPFDNERIVESVDISTFLSLKWSCIYYNIINYLYYKKGKTLLNFTLQRMKGKWTTIQKRMFNRIKHYYPNEEPKGYEINPKCFEVLENSSARNLIKEVALLKYQCSESSNIYKIYGDNQTELAIKLKELAQEYTPEHLHKHQIASALKSRFPKESSTEPEKLVVSKRLNGALDTDGSVSQEEREVEEEEPHGEQMEEDAEEGAAEEEEEEEEEEEEKSKHTEGSLQRKRKLALSDKDGQAENKKIIQPAKQQKLSSNSPSTLLDGEDDSDASASSDLPANFLETALQKSQSSNKKPNRKASQKKIGLDSTSQIPNTALLAESLSTMTVQDLDKNNELIVKKNELLANTINHYTLLLKLKHLQNELMDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFA5</accession>
    <name>A0A1E5RFA5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1752</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RFA5"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="152" mass="17391" checksum="D30C2A50C40029CB" modified="2017-01-18" version="1">MELNFKHNYSGYTSLLQQPTYPQNETIIDDLDFNFEYDTLNSTTTTTTTTTPTPKNEYTYTPYEDSNSVQSSASSTFSTDYNTISQQNKNDPYSYTFDMDSLIGTSPLSSVSSVYDYSESDINTNXNXSTNVNTKDIGTSEYNYRLWLNKMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFF6</accession>
    <name>A0A1E5RFF6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1772</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFF6">
        <property type="interactions" value="108"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFF6"/>
    <dbReference type="InParanoid" id="A0A1E5RFF6"/>
    <dbReference type="OrthoDB" id="6415790at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008521">
        <property type="term" value="F:acetyl-CoA transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035348">
        <property type="term" value="P:acetyl-CoA transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024371">
        <property type="entry name" value="AcetylCoA_trans_1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004752">
        <property type="entry name" value="AmpG_permease/AT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778:SF9">
        <property type="entry name" value="ACETYL-COENZYME A TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 33 ACETYL-COA TRANSPORTER -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13000">
        <property type="entry name" value="Acatn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="411"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="454"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="539"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ85631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="576" mass="64375" checksum="FA044A07291C6A81" modified="2017-01-18" version="1">MASLAPHDKPQFYLLIVLYFLQGIGCGLSYGSVPFLLKTLIKGSNFTQIGIFSMSTYPYSLKILWSPIVDSFYSTKRGRRRSWIIPTQYLIGVVFLVLGSSGILVRLFPELDPNFTTAHAATNLAQKAGSAVGSQTGKITSSINLPKLTCVFGFLIFLCATQDIAVDGWALEILSEESLSFASTAQTVGLNCGYFTSFTVFLSFNSSDFTNKYLRKIPKPYGLISLPQYLTFCGIVYMIITTYVAFYTKEKMLAPQGIQPLKQENDIEMAMPMXVSPVELPLLENSEFADESKLGEDKSQEFSIVQPKHTLTYVYRAFLKIIKLPNVQTLCAVHLVTKFAFQCNEGATQLKLLDKGFRREDLAITVLIDFPFEIIFGYYVGKLSSSNDSLNGSSSFFHTPNGKRRHFLIRLFLGELGVLTPFLFGFLGRLLAAALGSCVVWMYPSNSVDGDLPLWYFLLVIVHNLLGSFMNTIQFVSICAFHTRIADPIIGGTYMTLLNTISNLGGTWPKILVMKLIDYGTVVQPACVGCEETVVVQDGYYKTNFFCVTVGLLLYFGFIKKKMVYLQKTDKYSWRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFJ6</accession>
    <name>A0A1E5RFJ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ATP-dependent kinase YFH7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2066</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFJ6">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFJ6"/>
    <dbReference type="InParanoid" id="A0A1E5RFJ6"/>
    <dbReference type="OrthoDB" id="6362633at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ85679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="374" mass="42295" checksum="D8AD2B848B744FA6" modified="2017-01-18" version="1">MNTFNYKRLSTSVYYGSVKTASDPKISRQQQINQVTGKMSVHNETHNEIEHLXNQILQKVNNSANGERVYVVLVGAPGSGKSTIAEALKTCLLQKLAGSELLLTQSQQEEKSSNGFXXGTXSLEQVTSDIPLCAASDVPKHRLSEADLKAIENFKTFKYYGTDDDMIIESGGGIDNSIKLKVEFHSSATKXKNCINDYVSIVPMDGFHLSRKMLDKFQNFEEMHKRRGSYYTFDSKNYLELVKLITRVNKKRGTEDISIFYPGFDHALKDPQQDQHVVKPETKVVIMEGLYLLLDKPNWKSIVSSLPAENTLIYKIDSEESTMEKRVSKRHVQSGICATLEEAIERFRFNDAINGKFIEENMVPNKSIIHIXND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFL8</accession>
    <name>A0A1E5RFL8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1768</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFL8"/>
    <dbReference type="InParanoid" id="A0A1E5RFL8"/>
    <dbReference type="OrthoDB" id="10256176at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="3">
        <location>
            <begin position="202"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="149"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="46"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="163"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="384" mass="44427" checksum="5B636D1F03C46A26" modified="2017-01-18" version="1">MINPQQNVLSPDFCEAEKKLTSANIDDLDYDGNILNIVNLPRNKCFEILEKQLQTLVSSHKKLEDKLKDVETKLFSEKTKGNSGRYNNVVDLKQQRIELIKTINKLSLKINSCVLAQNYDSVFDNYTKVMLAPELANFLYRQELKEQQKKKQRQLSNEDQRLESQTGQQASEXGTSRSQSFQHLNQTNISALTENFTKSSIITKIPFMDNSFHYISIPGIWDTIPYKKWEPLAREVYRVLKPGGMLYSVVLDFKPKNLHSAAMSEFKTSREKEDIFNKVILQSTRSGHQLAPSQYLTQVLYKVGFKTVNYTYLSVKLGDLQTDLGKNNELLFFILFMLFFMNMGQNXKLKDIDTFAERYMKEHDQRIDTKXGATRILIAKAYKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGL2</accession>
    <name>A0A1E5RGL2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Altered inheritance of mitochondria protein 32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2195</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AIM32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGL2"/>
    <dbReference type="FunCoup" id="A0A1E5RGL2">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGL2"/>
    <dbReference type="InParanoid" id="A0A1E5RGL2"/>
    <dbReference type="OrthoDB" id="10253744at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009737">
        <property type="entry name" value="Aim32/Apd1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31902">
        <property type="entry name" value="ACTIN PATCHES DISTAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31902:SF7">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06999">
        <property type="entry name" value="Suc_Fer-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="297" mass="33403" checksum="BE77E3D51627A40D" modified="2017-01-18" version="1">MLKFARASRSLHTKYTFISIADALPSGLSKEAGAAQLSSLMKTCQCTLNNLVVSHSLPEKFAPLQIFENSSNVLHNSQLTNEVPSFKSHLLLISPTDSYAEWKSKLEMDSSTFPYNVIGPLKNQLKNCSIMVNAVSFRKSLDLAKSLQYEPTKIKFICIPEMVVVELPKNDKKALKGFTEYLECTYRENNQIYNPFTAFAKGEATQKLPSVTPHSLKSFTFQNIKDLNLIFVCGHAKRDQRCGVIAPLIMDKILEWRNKNKAYNYTPGMISHIGGHKFAGNLAIYEQDKTRVDGICH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGU0</accession>
    <name>A0A1E5RGU0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1426</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGU0">
        <property type="interactions" value="144"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RGU0"/>
    <dbReference type="OrthoDB" id="4084551at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028000">
        <property type="entry name" value="Pma1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14610">
        <property type="entry name" value="Psg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184744" evidence="2">
        <location>
            <begin position="27"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="416" mass="48003" checksum="D301608AB0402774" modified="2017-01-18" version="1">MYNSRKGSITQLIAVLFLLFXSNVLCFKNVVAPKEEKVTTTTEVLKPWVRTITQPTRTFNEVVTPTVIAGVTFSAKPPVSTDPLEPWVSLNKLGEPKTVRPQIKNGRTKNASPTYSTYFGNVYTTARTYEELGEPDNMDPSDVHEEEVFEDEDKTYVSLNPXIRCIPPRYFNKGAGKNIDSAPFCTPRENSELKVDHTYFVSWYTRFFTTPNEEDPLKDDQVEKVRIHLSYVQESAHEKGVKKRDSEIHEATFFVSEWLKNIDGIYPLTIDKEWLQGDYERKILMSIQPDSISDEDFHPLMEEVGPLTFKMILGSRVFKTSKNAIDLEHATNKHKPIWYIIMGTPCIVAVAVFGMYLFIKINSRYRDFSDVKNAVLQKRHKVIGKVKDMKKFQNMKNHRYDELPMYHLNKKKGKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHA0</accession>
    <name>A0A1E5RHA0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Transcription factor IIIB 70 kDa subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">B-related factor 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g1074</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">General activator of RNA polymerase III transcription. Interacts with TBP. Binds to Pol III subunit C34 and to the TAU135 component of TFIIIC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHA0"/>
    <dbReference type="FunCoup" id="A0A1E5RHA0">
        <property type="interactions" value="484"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHA0"/>
    <dbReference type="InParanoid" id="A0A1E5RHA0"/>
    <dbReference type="OrthoDB" id="511529at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000126">
        <property type="term" value="C:transcription factor TFIIIB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000995">
        <property type="term" value="F:RNA polymerase III general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20553">
        <property type="entry name" value="CYCLIN_TFIIIB90_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20554">
        <property type="entry name" value="CYCLIN_TFIIIB90_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000002">
        <property type="entry name" value="Transcription factor IIIB 90 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000007">
        <property type="entry name" value="Transcription factor IIIB 90 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.650:FF:000004">
        <property type="entry name" value="Transcription factor TFIIIB subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.650">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011665">
        <property type="entry name" value="BRF1_TBP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF4">
        <property type="entry name" value="TRANSCRIPTION FACTOR IIIB 90 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07741">
        <property type="entry name" value="BRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="16">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="363"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="502"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="364"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00074765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ86289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="613" mass="67998" checksum="313E7A42EB660CD6" modified="2017-01-18" version="1">MKCPSCGNTEDFVRDISNANNDLVCNKCGMVFEDNPIVSEVTFGESSSGAAVVHGSFIGVGQSHGGSGALESREQTLNNARRKLRAVSYALRIPEYITDAAFQWYKLCLTHNFVQGRKSQNVISACLYVACRKEKTHHMLIDFSSRLQISVYSIGSTFLKMCKHLHITDLPLVDPSLYIQHFAEKLDLSDKKNKVIKDAVKLAQRMSKDWMHEGRRPAGIAGACLLLACRMNNLRRTHSEIVAVSHVGEDTIQTRLNEFKSTQAAQLTIKDFRDNGADVESSKELPPSLKKNIKKSDKLQKNFKSFMSTDEETLKKDPIIQQLLNEQELSSKEMLFYLKKLQQLRTDTVSRIKKTHGIDDENVLISSTAPAPNGSSSQTRISDEDENAYQTVSTSHSVDDTNVDNALKKSDASEIDGYDINTDPYRPKNLHLLPTTDTLLSKVSDDPENLDDVDEEDLEDFILDEEASKLKERIWIGLNGEYLLEQESKRLKQEADIISGHANAMGENGTNGKKAKKKKMKRENSISGNGSFPPNVPDAASKRDPLGKFLSGGGEIDSKGNFQLLVSKNTLSKIDEDGNLAAGTTADSVKTMLQGNSSFSKKINYDAIDNLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHL6</accession>
    <name>A0A1E5RHL6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1537</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHL6"/>
    <dbReference type="InParanoid" id="A0A1E5RHL6"/>
    <dbReference type="OrthoDB" id="3972808at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="153"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="229"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="46"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="153"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="393" mass="43279" checksum="3BC9614A2BD70267" modified="2017-01-18" version="1">MAGKNTLKTNENSINNGNDTQRRNEQSLNFSTADQVVAESLQTKSPTKKTAKKVTKKRTKTPTQIKKENLETTAEVVAAATPGSSSSAAKNLYHRREAVCDRCRKWKRACYGVNAACNNCLMANHACTVSTPMIRKCKPKPVKILGLPITQSTNIPSKSHQKDSVSTSNMSSPVNTMATHASEPAGSLRKEPLSQLAFKMPVEEHPQYKKLLAENTALLNNEKKLIMDKSQVTKNLAQANQTITSLLEQMAVQTKKLEEANNTLLLTLQRQTQESFSAASEHLISIPTVHPKVAKNSYKYDSTSSSTVVGTPDNQNHTDSTSLDALFSTKAQPSTIFRGESFENLSQAKEMINLREEELIGDLGFPSKMSTIKNSIFLNDMDKDFLKTLGVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIA5</accession>
    <name>A0A1E5RIA5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Autophagy-related protein 27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AWRI3579_g1509</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ATG27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RIA5"/>
    <dbReference type="FunCoup" id="A0A1E5RIA5">
        <property type="interactions" value="88"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RIA5"/>
    <dbReference type="InParanoid" id="A0A1E5RIA5"/>
    <dbReference type="OrthoDB" id="29460at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018939">
        <property type="entry name" value="Autophagy-rel_prot_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09451">
        <property type="entry name" value="ATG27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0072">Autophagy</keyword>
    <keyword evidence="14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0732">Signal</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184763" description="Autophagy-related protein 27" evidence="17">
        <location>
            <begin position="22"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="233"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="18">
        <location>
            <begin position="26"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="200"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OEJ86630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="314" mass="34556" checksum="35BE1476B825A801" modified="2017-01-18" version="1">MNQHLLQLLISVSLLISVSHAGSVSLDCKKNSILQKYKLSQVSSVDELPVYHKTKNLPPSKVEESWFLNICSVQDNKKNKDRLSTCDENSVVCGNQKITLDDKQDDKTPSISEIFNIPGSVAPSDIYTVGSGDGGDGAGTSVGVSYKNIEWGTQSVDVAIKWQCDENMKNDMVKSVVVSEIPDTNIMQYDIVLNGPSGCPIDKKDNDNNGDDNNNNDEDKPKDPPKTKKHTSWFSFLVMYALLFTLIYLVASSYLAVRARNGNLMDFRIEFIGKLVTLIKACPALLKELFDKLMGRNRGSASDLSSSRGGYSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIY3</accession>
    <name>A0A1E5RIY3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">General amino acid permease AGP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1612</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RIY3"/>
    <dbReference type="FunCoup" id="A0A1E5RIY3">
        <property type="interactions" value="272"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RIY3"/>
    <dbReference type="OrthoDB" id="3970526at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF17">
        <property type="entry name" value="GENERAL AMINO ACID PERMEASE AGP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="362"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="423"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="447"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="489"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="530"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="6">
        <location>
            <begin position="91"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="602" mass="66597" checksum="1168E1FC667A0846" modified="2017-01-18" version="1">MSNLDKKPAYPVLSKEEDIIRIDDTETSSNYQTPKKPLLNSSLVQSFVDSFKRPETFNALGETNDLEVSPSNEIQDFGKKTELKQNIKPRHVFLIQLGTGIGTGLLVGNGSALKQAGPAGLIIGYVIVSSFIYCTYNSISELGISYSQLSGSYNEFSCILIDRAFGFSVAVIYALQFLCVLPLELTASSMIIKYWTTKVNPDVFVAIFYVLLVTVNFIGGSGYAEFEFVVNSFKVCMILGFIIFGIISDCGGSSGNNHTYIGGANWRAPVGAFFGSSKIDHFKGLCSSFVYAAFSYAGFESSILTASEQVNPRKSIIKAGKMLIYRIVIIYCLSIIMVGLLVPHNSSELLSTHSSTKSTSPMVIAAANIRGLPHIINAVILMAVLSVANNSLYVSSRVILSISEQFFPHIPLLTYVDRRGRPLFGLILGAIVGCIAFVACSPQETTVFAWLLSISGLSALFTYLSMNLSHIRFRKAMKAQGKSLKEMAYLSKTGVWGSYYASFLIILCFIAQFWIALVPVGSNKPDVNNFFQNYLAMVVFVVLYFGFKIVTKEWHIFIPSEKVSLSRGRRIFNQELIEQEKLEDAEKLRNSSLLKKILHFWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN05</accession>
    <name>A0A1E5RN05_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SUR7 family protein FMP45</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g621</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN05"/>
    <dbReference type="FunCoup" id="A0A1E5RN05">
        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RN05"/>
    <dbReference type="OrthoDB" id="5419460at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032185">
        <property type="term" value="P:septin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009571">
        <property type="entry name" value="SUR7/Rim9-like_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36414">
        <property type="entry name" value="PROTEIN SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36414:SF3">
        <property type="entry name" value="SUR7 FAMILY PROTEIN FMP45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06687">
        <property type="entry name" value="SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="113"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="190"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="294"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="445" mass="49210" checksum="3E348078E7090362" modified="2017-01-18" version="1">MKVKRTFDAIFLFFLLGAGLLTFFLILSGAKSTGTLTNFYWLKADTSGIDNANSETYWTNYMACGKSGSDFTMCSGKKAAYPFSPRDNFANSNDQLPTSFVQHRDTYYYLSRVGWAMLLVGLFFEIITILPNLLALCLDYTAILICSSVFIYFAWFFITLAACLLTSAFVKGRNAFHDSNRSAHLGVKNFAFIWTSVFLLTICAIWHPISATLTKRYNVLTKRNKNRWDNEDKEYDQQNENPTGAANYYGNPTGAFNTQTTVAAQQPVQPISRPTDYAGFTSSAADQNDVSDDNNNNNNNNNNNNNGTNQPARSATTNTNRTFVATGNPNNNTRAKANPSDPTDDSTVAGTGALNVPFQNKEMNNRNSFDESGEYDDSAEDDYTTDYDGKYATSPMTNPSSTTAAGSGLKNPIKITRSKKIKKRVPGTDKLIVTNVEDEAIVLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN11</accession>
    <name>A0A1E5RN11_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Telomeric single stranded DNA binding POT1/Cdc13 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g622</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN11"/>
    <dbReference type="STRING" id="56408.A0A1E5RN11"/>
    <dbReference type="InParanoid" id="A0A1E5RN11"/>
    <dbReference type="OrthoDB" id="4067010at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029365">
        <property type="entry name" value="TMEM238"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28613">
        <property type="entry name" value="SI:CH211-232M10.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28613:SF5">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 238"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="574"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="837"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="578"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="614"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="845"/>
            <end position="875"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1064" mass="121907" checksum="A2102F826C3DEA71" modified="2017-01-18" version="1">MVATFLNNTGELLQTWNEHGSCSVRFVSVLHSIKVVNGEINLLLKNFDGKEGKKDFYIARFAKFEHPCMQFFQHLKTAYKLNVPENFDKTHWSLKNIYLKSIIILNILGDIHATESFVYVLIDKFPRITNVSGNYGNSVNEFVNHLWTNLILMDDDKNQKFSFENLAVFFEPEKSFVAKLKSPPEEPRKTFSVDDINVQSLFSSDEEEEEEEEEEEENNREGEQDLRNLDFSFREESHAAKDVVESESSLNSQYSDSDSEFHDATSELLGLPLKRTAHEIGADLALLKKTKCDIARPTTLQNYGQDPVSLVSIATQTELTNADIVHLELFKDTQIPREVRKARYNMVVEVREPQTVPKAQSTQLSMIKNECPVVADTEDIYEELEPPNVKTEPPNVKTVSPVLAKVFPASVPLVQSFRELAKTMAHLSKTWEMSGSGVAQVRLRGSIVGILPTHQMIDVEDSAEKLEQVVFSFVLAKDNSSMVSKFHEKNSVFVTCTDVEWLCDVLDIKTFLKARHLEEKVSLMDFLDEIYLKTPFLEPCTVELQVQVSVHDGYRYELVPYMDDNGVAFKQNASEIESSNSNSNSNSNSNNNNSNNSNSNSNSRVVVFEPTGYQQQSQSQGNGDGHSNNTKTRNKYISVPIRRENTKYVKDVHVIGQNKMYLSIIGLLVSIDFVERTGQYAIMFSDFTFNNDAMLDTRFLPFFVEPHQMMENGTVFKSFMYGDRFNKMKSQFIDSFGDVMDFNTMLEAQNVTRYGVFCRFDLTVKLYNNKLDPLITNITFLDNLCVLPHDEQHFLIECRLKYFQNLTKTQIKDNLSLMKIFTNLQKNSDGSQYMIGSDEDKVIGNNTTTNTTTTTTNTNTNNNNNNNNNNNNNNNDLSNKKKDNSTIQDLDDNQMTLQIYKSSLQNMGAPPYRLDHSFIQTLERIEGKSVSFLTKILDQTNHLYFEVLGRIVSAELINKDMILKLRITDPINNQFPSKRNTVYDNENSLVAFVIFPSNMKYCFQEMNYCLFDKVVNKYILGQNSQFRLHRVPILTTSTTSQIIWVLLDFKISDIYDTFLHSMIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNK2</accession>
    <name>A0A1E5RNK2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glucoamylase, intracellular sporulation-specific</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g862</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNK2"/>
    <dbReference type="FunCoup" id="A0A1E5RNK2">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNK2"/>
    <dbReference type="InParanoid" id="A0A1E5RNK2"/>
    <dbReference type="OrthoDB" id="6123450at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616:SF9">
        <property type="entry name" value="GLUCOAMYLASE, INTRACELLULAR SPORULATION-SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="GH15-like" evidence="2">
        <location>
            <begin position="165"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="706" mass="81414" checksum="0B39E4BF5BCD2AC0" modified="2017-01-18" version="1">MTTIPEEKQFRPTVFTNAERKPKFLKKKWCLSYVSIGLFALIFTSSILAIGPEHTDIRKSEKKKVQLLYEKDSMKSFEKDSFEPHIQIIIDDFNLEPKVIDNEAKLYAKTKNDLHFSQYNSLVTQIKLKPKVRSIELSKGQFEQWIAQETEISFQRILDNIADTNLNTLIQSNVGMQQGVSIASPSKQHPNYFFQWIRDASITINTVINKLHYELQPDVMSRSRNPVFSGKKKNHSTKDNVHWLYFGTVLKHFNNSFVLQRTPNLSGQFSEQDLLGLGEPKFNVDDSAFMEGWGRPQNDGPALRVIAAMNLLNIMYLNNLQFETVTNVLQKNSIIDQESLNNMHFHNELEFFMGILYYDLKYIMGNWNQDSFDLWEEVKAKHFFTSLVQLKALKMAISFIENDVFGIFNDLDTHHNNNINFNVTDFHNQLEIEYDNLLNFMLFESGYISPGKNHIIETPLILQTRSGLDIGTIIGTLLTHDDHFVNKNLSDYDFPFDVDDSGVMSSLVGLVNEMRILYPINHARLNLDLGVALGRYPEDIYNGDGTSEGNPWFLATSTAAELCYKLVYRFYNNGQDLFIPLNHFNSKDFFWNTILDVPTTLNTFGVCNTCNSPQDFLSGPLTKRFLNYELIIKHNSPAYNATMYNLLAYGDSFLDKLREHVSGDGEMSEQMNKYTGFLQGANNLTWSYGAFWNSVQWRQNVYAIMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNK9</accession>
    <name>A0A1E5RNK9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tropomyosin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g782</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNK9"/>
    <dbReference type="STRING" id="56408.A0A1E5RNK9"/>
    <dbReference type="InParanoid" id="A0A1E5RNK9"/>
    <dbReference type="OrthoDB" id="3972566at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000533">
        <property type="entry name" value="Tropomyosin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00261">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="59"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="161" mass="19127" checksum="C1DB1175810CDC57" modified="2017-01-18" version="1">MEKIKEKLSSLKLEAESWQEKYEDLKEKMKELEQTNLEQENEVKSLTIKNSTLESEVEKLETELESSKKLEHESTNLKSIHDSTSSKNAQLEQELEETETKLKQVTDELKQLDLKAETFERKVTSLEEENRDWETKHEELKQTYENTKKELDEMLQSLNDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNT0</accession>
    <name>A0A1E5RNT0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Quinidine resistance protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g807</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. CAR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNT0"/>
    <dbReference type="FunCoup" id="A0A1E5RNT0">
        <property type="interactions" value="70"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNT0"/>
    <dbReference type="InParanoid" id="A0A1E5RNT0"/>
    <dbReference type="OrthoDB" id="440553at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF51">
        <property type="entry name" value="QUINIDINE RESISTANCE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="182"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="353"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="394"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="454"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="479"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="517"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="545"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="113"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="40"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ88528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="595" mass="66286" checksum="1B7B136E7983F461" modified="2017-01-18" version="1">MEQNERDGNMQTMSASLHSVLSHKPDTAARLSSSPLHKNASKDNESVPRDSIEHLKNNIHTSHEQDNNDFNDEKLSKTQEDHSNDDDSEHEMNKEDNEEYPYCVFPPRIVLMIVILCASEGLLSTISSSSFFGCLQQVRKHFNVSNETVNLAIVVYFICQGIAPTCVTFMVDSGYIGKRNMVLIGCIVYGCMCVGIANAKNFGTILGLRCVQSFSISPVIAVNSSIAGDITKKEKRGNIVGLVSGFQVCAGSAFGSVISAGLTNQFGWTGVFYYLAIMAYSAFVINFFLLPETKRSDCGNGSLLPARFIDRAPITYTSYWKTNFYSKPYYESKQAQKKTNILGFVEIIKKREVVFLLMASGIQFCVWTCHLTSISTVLSNSHYNMSTLEIGKCYLPSGVCTLFSIVGAGRLLNWNYKKRKLQYDEWIETQRDNEDFDISLPKYKFNIYRARLEFAHVPMLISGASFLICAWLMETEQCLASILVFSGVACLFSNCILTFSTTLMVDLHPFAGSTAAAVINLSRCLLSALFVGVLDYMFAAMGVGGTFILLFGITLIFSFGLAFTIKYGTEWSYNREHGIKNKQTLLSLLSFGYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNT8</accession>
    <name>A0A1E5RNT8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Actin cytoskeleton-regulatory complex protein END3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Actin cytoskeleton-regulatory complex protein end3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Endocytosis protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">AWRI3579_g774</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Actin patch</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the END3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNT8"/>
    <dbReference type="FunCoup" id="A0A1E5RNT8">
        <property type="interactions" value="126"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNT8"/>
    <dbReference type="InParanoid" id="A0A1E5RNT8"/>
    <dbReference type="OrthoDB" id="1716625at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000323">
        <property type="entry name" value="Actin cytoskeleton-regulatory complex protein end3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025604">
        <property type="entry name" value="End3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12761">
        <property type="entry name" value="End3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="13" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="12" id="KW-0967">Endosome</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EH" evidence="20">
        <location>
            <begin position="8"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="21">
        <location>
            <begin position="41"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="20">
        <location>
            <begin position="173"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="106"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="291"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="132"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="291"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00029684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="OEJ88541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="424" mass="48661" checksum="D15C1070CFF53121" modified="2017-01-18" version="1">MPKLEQFEIKKYWQIFSGLKPKDNKFLSYDQVKPILFNSKLDSSVISQIWNISDIDDDDCLDFEEFVICMRLIFDLVNKTISEVPKRLPEWLIPGSKVGLLKEKEKEYTNEQPQSEPASAPERTSPSQVTTSAESVQEASQSSRPAQQLQESKKEEEPEEERPDVDWYISPQDKGLYETLYNTSTLNMDGTISFPSLVNKLKYTILISNINNIQEMDMIKVWNLCNPSKHESIDKDPTLYFMHIIKQMNDKNCKIPVEVPESLKKSFSKDQVETNINSKQSIVKKTPIYSTSVSGNSSTVSSSSKKLRDGTDFSTTENTDWEIVRLKRELNVLDAEYDSVMQTSMSLTQGDQNLKTLKEQFKGLLHHEQKKIINQSESKGTSQQNAKLIDDELDNIEEQLHVLEDYLGSRKDVLNTINQELATF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNX4</accession>
    <name>A0A1E5RNX4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g743</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNX4"/>
    <dbReference type="InParanoid" id="A0A1E5RNX4"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005199">
        <property type="term" value="F:structural constituent of cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000420">
        <property type="entry name" value="Yeast_PIR_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00399">
        <property type="entry name" value="PIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00929">
        <property type="entry name" value="PIR_REPEAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50256">
        <property type="entry name" value="PIR_REPEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184827" evidence="3">
        <location>
            <begin position="18"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ88568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="90" mass="8730" checksum="2A06FFDB9C32BE2E" modified="2017-01-18" version="1">MQFSTVAFFSLAAIATAESVAVVSQIGDGQIQATTSAETPSETTIATVSATSSTETNNGTTVATESANGAVRYGAGAMAGIVAAGAALLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNX8</accession>
    <name>A0A1E5RNX8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribose-5-phosphate isomerase</fullName>
            <ecNumber evidence="4">5.3.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">D-ribose-5-phosphate ketol-isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Phosphoriboisomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g687</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>aldehydo-D-ribose 5-phosphate = D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14657"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="EC" id="5.3.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ribose 5-phosphate isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.6" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNX8"/>
    <dbReference type="FunCoup" id="A0A1E5RNX8">
        <property type="interactions" value="905"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNX8"/>
    <dbReference type="InParanoid" id="A0A1E5RNX8"/>
    <dbReference type="OrthoDB" id="1555531at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00412"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004751">
        <property type="term" value="F:ribose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006014">
        <property type="term" value="P:D-ribose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01398">
        <property type="entry name" value="RPI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004788">
        <property type="entry name" value="Ribose5P_isomerase_type_A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001924">
        <property type="entry name" value="PRK00702.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00021">
        <property type="entry name" value="rpiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934:SF0">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06026">
        <property type="entry name" value="Rib_5-P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75445">
        <property type="entry name" value="D-ribose-5-phosphate isomerase (RpiA), lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ88413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="342" mass="36579" checksum="572CB6D5CF21258D" modified="2017-01-18" version="1">MNPAHFSSAAALHSVFKPNNMPGVGTPIGTPIGTPIGTPIGTPIGSPIGTPSGTPVHAPALVTASGLRTQLCNVEHLEQLHDPLESSKRLASYLAVDNHFLPLVAAKYSQQLENASTANKADDTPLVVGVGSGSTVVYCAERLGEIKRTFPEILHDLNFICISTGFQSKNLIIENGLRLGLIDNYDKLDMAFDGADECDENLNCIKGGGACLFQEKLVSLSSDKFVVVADYRKKVKNLGSKWSIPIEVIPNSYKYVENQLAKIEGFQKSQLRQGGQFKAGPVVTDNCNCILDVNFGQIANPAELHSKLIQIVGIVETGLFPNKNCIAAYFGNSNGTVDSIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNY2</accession>
    <name>A0A1E5RNY2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">4-nitrophenylphosphatase</fullName>
            <shortName evidence="5">PNPPase</shortName>
            <ecNumber evidence="3 5">3.1.3.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g597</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2 5">
            <text>4-nitrophenyl phosphate + H2O = 4-nitrophenol + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21664"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57917"/>
            <dbReference type="ChEBI" id="CHEBI:61146"/>
            <dbReference type="EC" id="3.1.3.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Divalent metal ions. Mg(2+) is the most effective.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.41" evidence="3 5"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNY2"/>
    <dbReference type="FunCoup" id="A0A1E5RNY2">
        <property type="interactions" value="548"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNY2"/>
    <dbReference type="InParanoid" id="A0A1E5RNY2"/>
    <dbReference type="OrthoDB" id="413953at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000039">
        <property type="entry name" value="Phosphoglycolate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006349">
        <property type="entry name" value="PGP_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01452">
        <property type="entry name" value="PGP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF46">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000915">
        <property type="entry name" value="PGP-type_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="30"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00050247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00066659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00069197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ88587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="313" mass="35159" checksum="3FD32B194BC72900" modified="2017-01-18" version="1">MTVSNNTPIKISDKQIATEFLNKYDTFLFDCDGVLWSGTHLLPSVVETIEMLKSLKKTLIFVTNNSTKSRKQYTKKFESFGIKDITEKQIFGSSYATAVYIKNYTDLKPGEDKIWVFGEQGIEDELHDMGFETLGGSDERLMAEWNSDTTPFLPLDPKVKCVVAGLDTKINYHKLCISFQYLTQKEPKTQYIATNIDTTFPSKGMLLPGCGSMIDLLTVSSGREEPPNCGKPSQKMLDTVFKAYPELQRSKCCMVGDRLNTDIRFGLEGNLGGTLLVLTGVETEDRLLQNSDHGLGLPKYYANKLGDLYEFTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP07</accession>
    <name>A0A1E5RP07_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Serine-threonine kinase receptor-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g920</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat STRAP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP07"/>
    <dbReference type="FunCoup" id="A0A1E5RP07">
        <property type="interactions" value="1118"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP07"/>
    <dbReference type="InParanoid" id="A0A1E5RP07"/>
    <dbReference type="OrthoDB" id="24966at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002183">
        <property type="term" value="P:cytoplasmic translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027525">
        <property type="entry name" value="eIF3i"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877:SF1">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24805">
        <property type="entry name" value="EIF3I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 9" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="6"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="48"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="286"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ88621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="339" mass="38207" checksum="BEDC7F750DFC156D" modified="2017-01-18" version="1">MRPIQMFGHSRPVSQIRYNRDGDIIVSVSADESASIWYSNSGERIGTFNGHNGSINSVDISSNSKFCCTGSGDSTIKIWKLENGQELQTIEYLPYIECVEFSPVDNKYLLVITAGVFKTHGCIDVYAFDNETGKIGEIVFKIERNPEVDSFMEFKRASWSFDSKYIIIGCKDGQILKYDVEARKIVHQEQLHKLIINDIQFSPDRTYFITASKDRFSKMVDVMTMKTLKQYESTGPLNSACICPLKDFVIIGGGQEARDVTTTGSKEGNFESRFFHKIFETEIGRISGHFGPINGLAVSPQGNNFASASEDGYIRLHHFEKNYFDFKFDVEKTQAALAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP36</accession>
    <name>A0A1E5RP36_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Required for respiratory growth protein 9, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g606</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for respiratory activity and maintenance and expression of the mitochondrial genome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RRG9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP36"/>
    <dbReference type="FunCoup" id="A0A1E5RP36">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP36"/>
    <dbReference type="InParanoid" id="A0A1E5RP36"/>
    <dbReference type="OrthoDB" id="5578174at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010487">
        <property type="entry name" value="NGRN/Rrg9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13475">
        <property type="entry name" value="NEUGRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13475:SF3">
        <property type="entry name" value="NEUGRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06413">
        <property type="entry name" value="Neugrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="194"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="211"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ88644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="239" mass="27771" checksum="2865849385AF1307" modified="2017-01-18" version="1">MLTLKTRIQRFSSCAIPKKSFEARKLLTLVNSSSDIPKRNSRIGTDSSVSKNLLKHHNNQSVSNETNGEHVAVEKGLTLVQSASDSYTGVGKTTRKKPEWLIQKLALKSKFENWNPTKKLSRDEMDNVRLLKEKMPYLNNTDIGNHFKISPEAVRRILRSRFTPKTERERQGLENRWMRRGERIQVQMDELAQQSRGVKPDQFPTKQTRFFKKDSEEISRKFQNKKQSKVLESLKKISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RTP6</accession>
    <name>A0A1E5RTP6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g572</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000004">
        <property type="protein sequence ID" value="OEJ90249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RTP6"/>
    <dbReference type="InParanoid" id="A0A1E5RTP6"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="78" mass="8771" checksum="25A467807552FFEC" modified="2017-01-18" version="1">MNSWSFNDKGTKIDSQRLEDTGIAMKSIVAMVMQVRRDRRSRQRDMADGMGTGVSMDTPGLNEQANTDAMTVDWENDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV26</accession>
    <name>A0A1E5RV26_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein CSF1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g393</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RV26">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RV26"/>
    <dbReference type="OrthoDB" id="10051416at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006113">
        <property type="term" value="P:fermentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029636">
        <property type="entry name" value="Csf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056779">
        <property type="entry name" value="Csf1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048636">
        <property type="entry name" value="Csf1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32085">
        <property type="entry name" value="PROTEIN CSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32085:SF3">
        <property type="entry name" value="PROTEIN CSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25038">
        <property type="entry name" value="Csf1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21678">
        <property type="entry name" value="Csf1_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="2">
        <location>
            <begin position="1689"/>
            <end position="1728"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="1213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ90790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="2966" mass="339064" checksum="BD10FCA7C054BE0B" modified="2017-01-18" version="1">MSPKILTELTSNSWSYNWVFLADWIMVVILITFYMFYLNRVFGFLLTKALNIVLWKFWKTYIXIESISISLIGGKVDLKNVSFVTQDFTISLIKASFNWKFWLLHPSVPTIFNKKEGYSETAMKQGSRNVFHIEGFEIFIYNRSAAYINIIKEYFSKEENAELQKQFPELYKNFMNLEEDEEYEVNNATSFDKKPQVPYEEALNTLSPSYKWMLKCLPVIISVKLGALVLGSINTPSVLIFHWKSSDSLIDAQKADSPLDKLKFKLTTTHFDSKVVLKPNPLYESDNQRFKGXMYRKIVKSMKNASAFILHHRFKNKSLHPEEIVQQWKGLPMYTEIEDDTVNQLENEAQMLVSHQNEYAKFSKVLECDKVIHTYSFDVAGRVLSEKAETSTEFQGPDIGNGGSPPDHRLDLQFFEAHLYYGPWASRQLAPIIKVLSPQITRDHKKKKVLLPGSTRLATRFQLFIDISSKSTLRIPTREFSKDNLFLKNYSKTKNAVRPFGWIDIDLNMGSSAFFDFTFFPIGNNYVNTFNIDLMTPTMYTSVNHDIFFRADYHNFTAQSFCPLKWNETVKWQFNQISNNSEIFLLREHVTLLLDMFQDFSFAIDNGSFYDLYRACEYNMSWRFKETYKIHVNVNDANVINIATDFNENSYVTVCGESSVINAKTLLNNIAALSTEIFFDIDTPLFQILLNTPTWSTISNFISGREVGRSANFLLNGTYKFFHNVDIGNVDQLVLECESDYTTILLFGFVIKYFDNLRANIVGKFKQYKTSEEFQEAQQELSTEKSLNFARSGEESDAESFVDTITSEDSESSHFGNEATVKKSELRRTSNETDIWITFCVNDGHIFLPENLYDSSSCLGLHFPSLTSDLRNLDFYFDMKIEALPVFFKRHSFEEGINMFDVFDPTKLSEIVSQNDGYLENIFVHGQKFYGVPPNFDLYMTKWDMSIGNLSLDSDLSFYKCVYEAFSKIDFSSSDVENTLVYETSSLDTVEFCSFEIAQVEVRVREADCVFXAEITDIMISARDPDNTGLSKKFNVNVNEITCRFFTDEMKVFEVSTALNFTNFEIPIDFEAKLKKQRENLLLEDAPYHRCWFLLPKELRERIVYSDLIGSVPSTSSIPSLVTPFTADTADYIIDGFLKGDMHLRDYLFNETEYDQPDSINSTATGHSADVAFCDNDFVDMTKRETSNAVWQIEKTTAMFDISNLGKIIQFLDTLSETSXENIADSIQMSVVSPLATITKMISTDSKMNVESKEICISLKNSAFDRDTSLILNFSNLNYMCKETKVFNQARSRWQYEYEKSSACTFDNCVIRFDREDLRNVIGHPRSFRIILDSFEFLSDCDEIDVSNFSLGTIKVQMNSASNEILFQMAKASLNSVKMELPKLQRVIERVKTQKREFILQLLKASVKYEINYDPPVITKLSNIVKLSKSHVRDNPSWRMSVRIRHILKHVPPGWEDNMLSILANNNFTPLQKASDDFVGIFKKWKNFEQRESCDTYLYHSLFLGESSFKPVTQNISKVWAFSLDKLLLTNALLDPLDEDTIFVKFFEFKYSDYFQHLPNTSKNTDKESSLVAGSIDNAYFAKIGRLKANLGLPLLETLSLYQSLMDETTTTNETQIQTKSETLLNLLNDHCLFMVDSLDFSFNFDSKSIHCRSFGNNGSLMKTEDGGSLALSYLNFDFFLKYHNSILLHYGIQKGSVYYGFLDDVEGKSKKKIDLFGNDVIIQSREDVKKGLHILDSFISSFKQLGEDYCNLVPKKEDANSFETSEDVWVCSLVLTEIQTSLYIFSPFKVDQNVEYFETTLSLDDVKKIELLFRNANATIASSSTKQAYFLHSQSEFAATLLPSCSSAESIMHDIAIRSGLIKNTVFDLRDIGKSLSADINVFLNTVSTMKSFLIKHKVIDPDSDLGFLFDLAVRSDYLGVLFDFKSYQLIVEVEDITAGVKNFVEETHGIAQILDFLWYLFIDSFKVSVKDPQLPIEFTQVLNFAIGASVSREVVDELSSLEIKSKYFRVCLVPQSFIRVFWMYEQFMNFMDQCDLTPKNSIKKTTHGNATSKNQQPKPIEFTTRFKNVQCLAYDFCIGWLYGEPTNIYTFDDKTDSGLIIGFEKLFSAHNKTLGKLTVCNSYTSVSFGESYEDFYGLKNDKTCVNKTCLPNIQVAYWLTKNDGSTHDLNLKMWGDLLQITLAENIVDVIQRVTKSFLRVQKLKNYILKGFTKSAKLSGARASGDSLKNIGLRHVNCSVNYAGGHCCIYSNRDILSGAPPTVSLTSPEVDFKIKYEHQPDSSFVHAFRIIINVGKAQNVLFPKCVPVFHQMVKDFQGLLTLLGPDHDINEPVSPTSAKNMDYNKLLEKFDIALTVEFEKQDLSISCEPKAKIKAVFGFEHILINALTSNEDPLLSFSVSLNISGLSSKFQHIFSKNVSSSFSIANLNTVFLVSHKDYTDVYAITKSDKLKVFLDFNQFEDLMLFFDIWTPHSVLSSTNEDIDMGPKNNFSDKPKTYAIDPTLAWNYIIIFDETEGKFDLGPSLGVLTLSSNRMSASLNQFIDWRKKISLDIDDLTINSEGRLSGFVSLAEFNWTSEIKWDRGRGHRFTPLVYLSCSLGNIRTKISLDYHIFLIAHICQTKLTVFNTQDLSVSTQGLLAASLNCDKIELFSTILTTSNILNIYDTVTRIKMDNRMSYFETLRDSNTKTLADHFIDKELENYISSLKTDLSVNLGELYVQVFEHTLTDTDVLVVRNDNTRASMSTKGKQKIATQLEMFTNKLTVKVSSNEFPIKDKDLANTAIEDYMKMARKAHGGTLVSFPSINIQMTTWQSVGSNIIELLYSSEFGGMVSIKWNLRFVNFIRDMWAAHAVTLSQRHEELLAAKEEEEKSEESSGRGATSFFEEKDLDNKIKNVDLGDKYVYQPLAEPHIETPQIKDLGGATPPLEWFGVNRNRFPGMVHQIVIVPLQHVIAQSERQYGSALSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV31</accession>
    <name>A0A1E5RV31_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g399</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RV31">
        <property type="interactions" value="965"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV31"/>
    <dbReference type="InParanoid" id="A0A1E5RV31"/>
    <dbReference type="OrthoDB" id="1666796at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF111">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009028120" description="Transmembrane 9 superfamily member" evidence="7">
        <location>
            <begin position="22"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="330"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="365"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="402"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="435"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="589"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ90791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="637" mass="72809" checksum="03CFB77D11EA7222" modified="2017-01-18" version="1">MLSANVLSFFLVPIWLRLTLAFYLPGMAPTTYQEGDEVQLFVNHLTPGLREQNNKENHIFSYDYYFHKFHFCEPDKITKQPQSLGSVVFGDRIYNSPFKINMLKDTTCNKLCSSKIPKNDAKFINQLVSEDFRQNWIVDGLPAGREVLDKSTNTHALSSNFELGFFDSTNEANKYFKVPLFINHFDIVVEVYDIDASHYRVVGVRVNPLSLERNVKDSCDLTGKPKILNEKKDNYVDYTYSVKFEHWDTPWATRWDKYSHTSNSQIQWFSLVGMSVAVLFLVTLMEQILFRTLKKDFSRYEALNLDSDEFDQDSGWKLLHGDVFRAPKKLLLLSSLTGAGIQILVMSGTVILLACFGLLSPQNRGALPTFAIVLYALFAFIGAFTSMTVYRFFGGXQWKTNLILTSSLIPGSVFLTIIFLNVFLLCVHSSDALPFSSLCMLVGIWMLLSLPSSVLGSLLARKLSFKDNAFIFSSAPTKTNQIERQIPIQPTYLKTIPSALVAGIFPFGYRNASPGSSFELKFKHNTLNTSKKWSRDNMFSXQWFNIKDIWAVPGTDSSISYQKKMDRIDTKETEIEKKVLSEAQELQKKQLENDTIALLKREIEAQKENVSRLKKENKYLQEYVENLMDSIDLTKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV55</accession>
    <name>A0A1E5RV55_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="23">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">AWRI3579_g418</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 23">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38567"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:38568"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphocholine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38583"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:38584"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine(out) + ATP + H2O = a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66036"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:22801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:66037"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 22">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="23">Membrane</location>
            <topology evidence="23">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 23">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="27">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="23"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RV55">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV55"/>
    <dbReference type="InParanoid" id="A0A1E5RV55"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070867">
        <property type="term" value="C:mating projection tip membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        </source>
    </evidence>
    <sequence length="1662" mass="188184" checksum="AC6EA65256808B52" modified="2017-01-18" version="1">MNNHDSXNPLFNTXNINDNNNNNNNLFDVSLDDDTNTNTNTNNYSRLNGNNTTSSPTNPFSNDNSIDNSESSSDHAKKHNQHITISTNVKAEDGLHVIENDENKEEQEGDDDDDDSIFGDHTKTPTTHQHARFADDFDDFSDSGEFDSDGDATKKGSKTQDHHQHTGMKRLRWATRRHKDGKPKVGRAKTLKWAKNKFPIHSMNQLYSSDDVDVDDLAGESKNRSAEKRTIFFNLDLPPNMVDEEGKPIVVYPRNKIRTTKYTPLNFVPKNLLLQFNNIANGYFLFMVILGAFSIFGVTNPGFSAVPLIVIVCITAIKDAIEDSRRTILDLQVNNTSTHLLTGIENFNCSDDNISAWRKFKKTCTKVTWACLKYVSARLTKKGRAELLREKHKRELRQMNDNFQMPRISGNGRGSFDSVQDYRPSLDDPRASMHYNAAEMKKFGNLIDYNMPYDDECKFKKSFWKDVKVGDVVRIHMNDEIPADVILLSTSDPDGACYVETKNLDGETNLKVKQSLKCANLIRTSRDIVRTKFWLESEGPHPNLYSYQGNLQWNERQQDQNSHGGSAPVLRQEPVTINNMLLRGCTLRNTKWAMGLVVFTGEETKIMLNTGVTPTKKSRISRELNWSVILNFFLLFCLCLIAGIVNGVFYDENGTSRKYFEFGTIAKNPAANGVVSFFVALILYQSLIPISLYISVEIIKTAQALFIYGDLLMYNEKLDYPCTPKTWNISDDLGQIEYIFSDKTGTLTQNVMEFKKCTINGVSYGRAYTEALQGLRKRQGVDVEEEGAREREGIKQDKEFVIENLPKIGSGNSQFFPGEITFISKDFVKDLEGSKGDYQKNCDEHFMLALALCHTVLTEPSKTVEGKLDYKAQSPDEAALVCTARDMGYSFITRNKTGVIVEVQGVEKEFEVLNILEFNSTRKRMSCIIKIPGDKPKALLLSKGADSIIYSRLSKTGNDEALLDATAKHLEEFATEGLRTLCVAQREIEWEQYLEWNARHDAASSSLNDREGAMERVADEIEQEFILLGGTAIEDRLQDGVPDSIALLGEAGIKLWVLTGDKVETAINIGFSCNLLGNDMELLIINDELSEEDKENSSRRVLEDDPVTIVERNITRYLQDKFGMSGSEEELEEAKKEHEVPKGEFGVVVDGNALKLALMNKEVSRKFLLLCKNCKAVLCCRVSPAQKAAVVKLVKDTLDVMTLAIGDGSNDVAMIQSADVGVGIAGEEGRQAVMSSDYAIGQFRYLTRLILVHGRWSYKRLSEMIPCFFYKNVVFTLALFWYGIYNDFDGSYLFEYTYLSFYNLAFTSLPVIFLGIMDQDVSDVVSVLVPQLYRSGILRTEWTMKKFWGYMVDGLYQSVIAFYFPYVLYSKTGLVSANGLGLTSRYFLGVPVTAISACSCDLYVLMEQYRWDWFTTFWVAISILVFFGWTGIWTSALRSGEFYKAXARVFGAPSFWAVLFVGILFCLLPRFTFDVLQKMFFPRDIDIIRECWKRGDFDQYPPDYDPTDPHRRKISKYSLDALSQIEDSYLVDDQEKSSTGILDEDVNPMQTRNKXSHNRVKSVLSMIDESVPILSGRHVSSATTSSPFENELDNDIGLANGSRINSGNNKYHQSMDTIQTEEIPLDIMENHTRGTSTNDXRTSTDQYTRYSQDYHRRTSPFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV71</accession>
    <name>A0A1E5RV71_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Trehalose-phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g453</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the trehalose phosphatase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RV71"/>
    <dbReference type="FunCoup" id="A0A1E5RV71">
        <property type="interactions" value="239"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV71"/>
    <dbReference type="InParanoid" id="A0A1E5RV71"/>
    <dbReference type="OrthoDB" id="755951at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005946">
        <property type="term" value="C:alpha,alpha-trehalose-phosphate synthase complex (UDP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01627">
        <property type="entry name" value="HAD_TPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1020">
        <property type="entry name" value="Trehalose-6-phosphate phosphatase related protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00685">
        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF123">
        <property type="entry name" value="TREHALOSE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="76"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="248"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="441"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="79"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="256"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ90850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1120" mass="126683" checksum="79E4C558E159A14B" modified="2017-01-18" version="1">MESTKSENPGKSNSQDTNGLKSKTKTQTKQTPFSGRVINCVARLPYNITKVINKRGIVVNTRGDIDDINEYYADRDESGGGGNVGKNGGNNGQIPQVKDGKPHFKTNANAPTVAEEIEMIASKLDKDLKQNEYEKRERMNDVKYQKKYEGDENEENLTFEEEEEKETIQWKIDPVTGNSALYSSLKYINEVVVGDDGNEIKKEDIVNEIIVGWTGEINTQEKIHYEEPDSINKASPEEHNKNVTVVTKNGNDHSVTTTSTTSTTSTTTPNASGNATGIAPNREKHNTNALNDDDDVDGEEQELEEAFKLSTTGDINLLNNADDPLFLTQENKDYLTKELNNNPSQPNISVEPVWLLRKDQLKWRQYAENVLWPDFHYILSPIQDSCNIEKNWWFDYVKFNEAYAMKIAKIYKPGDIIIIHDYYLMLLPQLLRMRLKASNNDEEENSSNEDGLSNEEGSSKPDVEAHVSIGYFHHVTFPSFEYFRCLKYRKQLIDGVLGADVVFFQDYSFQRHFVSCCKRLLDCTAKKDLDTNNHHVSAYGADILIKTMPIGIDKEKLIAKTFNREIDLKVETLRTAYAGKKIIFGRDRLDSVRGVLQKLQSFDIFLEMFPYWRDKVVMIQVSTPTGLISSSSSNDNKLERQVMELVNSINTKYGNLDFSPVQHYHMRIPKDIYLSLLRVADLCMVTSIRDAINLTALEYITVQSHESEVNKTGKAIKPLILSEFSGTPLNKEDCYIINPWDSVAVAKTIDNALQKQYFQVSKEIEKTAAKVPNMLDWTSELVHTLEGFLTSTTSMTPALNRPCLYKIYNQAERRLFLFDYDGTLTPIVKDPAAAIPTAKLYSLLQALAKDPKNDIWIISGRDQAFLNKWFGTKVPEVGLSAEHGCFIKNSNSTEWVNLTEKYDMSWQKICGDLMEHVTSKTPGSFVERKKVALTWHYRGTVPELGEFNAKELKEELMKNFGGTDIEVMEGKMNLEVRPKFVNKGEIVKRLVLTKRGTKTDMLQSKTTLDDLTQQDLPDFVLCLGDDKTDEDMFNQLNSIESGFATANKKSPSAKKFIKNPFGNYGFFPTSVGSANKQTVAKAHLTDPQQVLETLGLLVGHVSLFESAGSVDLDDRGHVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV80</accession>
    <name>A0A1E5RV80_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GTP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g510</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">GTPase involved in activation of the TORC1 signaling pathway, which promotes growth and represses autophagy in nutrient-rich conditions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the GSE complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the GTR/RAG GTP-binding protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RV80"/>
    <dbReference type="FunCoup" id="A0A1E5RV80">
        <property type="interactions" value="603"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV80"/>
    <dbReference type="InParanoid" id="A0A1E5RV80"/>
    <dbReference type="OrthoDB" id="26136at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990131">
        <property type="term" value="C:Gtr1-Gtr2 GTPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11385">
        <property type="entry name" value="RagC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001086">
        <property type="entry name" value="GTP-binding protein GTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006762">
        <property type="entry name" value="Gtr1_RagA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039400">
        <property type="entry name" value="RagC/D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259:SF2">
        <property type="entry name" value="GH16429P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259">
        <property type="entry name" value="RAS-RELATED GTP BINDING RAG/GTR YEAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04670">
        <property type="entry name" value="Gtr1_RagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="23"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="10">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ90841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="433" mass="49105" checksum="E6823FE74ADA1DE7" modified="2017-01-18" version="1">MSKYGSRDYTTGHPNSKHERTDNFQSTNGIDQNHNTNEGYYIDNSDGRAAGSMHVGDNRALEDEGDNNNNNNNDNDNDNDNDVDGDTEATHATVLLMGLRRCGKSSICKVVFHNMQPLDTLYLESTSKPTVTSNKLNKNLINLSIMELPGQLNFFEPNYDHEKIFKKVGALIYIIDSQDEYLNAITNLAMIVEYAYKINPRINIEVLIHKIDGLNEDFKLDTQRDIMQRVGDELLDLGIDNQNGSGISINFYLTSIFDHSIYEALSRIVQKLIPELPFLENMLDNLVLHSDLEKAFLFDINSKIYVSTDSSPVDIQTYEVCSEFVDVTLDLEDLYCVNLDSQQHGGRGQNKLDKDKEIMSFSKLNNGLCIYMKEMVRGLALVALVRLKNEDTTNNANSGAIQSSTKTINEYLTVIDYNVEIFKKSLEQIWKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVD3</accession>
    <name>A0A1E5RVD3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">APC/C activator protein CDH1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g543</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat CDC20/Fizzy family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVD3">
        <property type="interactions" value="779"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVD3"/>
    <dbReference type="InParanoid" id="A0A1E5RVD3"/>
    <dbReference type="OrthoDB" id="3971552at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF1">
        <property type="entry name" value="FIZZY-RELATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="7">
        <location>
            <begin position="433"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="563"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="605"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="693"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="111"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="229"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="307"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="752"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="31"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="231"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ90907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="792" mass="87772" checksum="394D45136E796398" modified="2017-01-18" version="1">MSEQKNNYKRRQVNPFLVNTPDPSPQKPGLTPTNSNAASGIKRTNTDSCYGSPLKSFQQKETTVHHQYTNSPLLGSPLKKSKSGSASNSPFTDRLLPRRTDVDLQSLKYLDGQRKSNQLRFENSGSPVGRPSNANRTGLAEQDTEITVDSENQLELQKLKQADQLYDIILKNELFGDKLEGLTGGSNSKYENTKKTQGTLDIIKASSASSTSKSGSRTDFSAVFEDHHQDNMSLELSPTRTVGSQRRNGGARTTQSRAHMTSSVSMPSLQLRRHNLXLAGSSEELANDTVRVRRQLNFAQFEDFSSDDEISVSSDENSFGNNAHLAGQHHQQPEREASRLHQGPFGDLNFLKTAEQPKACLMKGASLLQYSPDSSXIPVKCPSDSLANMKKLMEQNREILSSRAMPVRPESKKLLLTPSKRLRDIPKTPYRVLDAPGLADDFYCDLIDWSSQDKLAVVLGKSCFLTDDVTNDVSELCSLKNESQLSYTGLQWANSGSHLALGFSNGLTQIYDVVKNKCIRTLSGHTDRVSCLSWNNHVLSTGSRDSNILHRDVRSPDHFFETLVTHQQEVCGLKWNVPENKLASGGNDNIVCVYDGVNKNPVMKMEEHRAAVKAIAWSPHKRGLLATGGGTADRHLKVWNVNTSMKLKDVDSGSQICNLIWSKNTNEILTSHGFSKFQLTLWDYPTMDPVATLKAHSFRVLHLTLSNDGTTVVSGAGDETLRYWKLFQNXKAKRHGQQATHLQQLERGLARQEQGFKPSGSTSSNQRGLDDDDGDDGTNLNSALLDTFKSIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVD5</accession>
    <name>A0A1E5RVD5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Actin-like protein ARP6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Actin-like protein arp6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g395</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. Involved in chromosome stability.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the actin family. ARP6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVD5">
        <property type="interactions" value="348"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVD5"/>
    <dbReference type="InParanoid" id="A0A1E5RVD5"/>
    <dbReference type="OrthoDB" id="6220758at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10210">
        <property type="entry name" value="ASKHA_NBD_Arp6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000040">
        <property type="entry name" value="Actin-like protein ARP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ90785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="440" mass="50448" checksum="3349E4CCA0C95376" modified="2017-01-18" version="1">MKALXPLVIDNGSYEIKFGLAXQGQKPYHCYNCVAKNTNLGTESFYIGNQLDSKLQNSTSGVYIKRPIERGQLTSWEIENEVWNYCFYNPEEFGGFELSNNTNERQDLILSETIFTLPELSRNTDQIVFEEYAFDSLYKAPSASFVPFNKESILRNMLLNSKSQEIPDSNVKVDQSTTTQSSAMTSYNDFQLVIDSGFNCTWIIPIIKGTVHYKAVKKCDIGGRFFTGLLKEMLSFRHYDVSEETILVNRIKEQCCYMSPQSYTESFRNKNDSVVEYVLPDFHSDNRGYRFDPATSLPLPQTAQTLVLTDELFAVPETIFHPEIAQLVNKPGIIETILECLSMVPEIVRPLLVGNIVLIGGNFNIVNFKERLSKELQVQCPIDWEVRVSCSAQPSTFTFESMCKFSETDEYIHARVTREEYYEKGADWLSKNRFTYQQSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVF5</accession>
    <name>A0A1E5RVF5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="7">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g449</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PPP phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVF5">
        <property type="interactions" value="111"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVF5"/>
    <dbReference type="InParanoid" id="A0A1E5RVF5"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07415">
        <property type="entry name" value="MPP_PP2A_PP4_PP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033096">
        <property type="entry name" value="PPPtase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="9">
        <location>
            <begin position="181"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="103"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="6">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR033096-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ90874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="383" mass="43473" checksum="81B9DE321AD4EDD1" modified="2017-01-18" version="1">MLENIDKIIEQICRSTSSGSQHSGFSAANDNNNNSSXMLPEETIYQLCQLAQDLLIQEPNIAHIDTPVTICGDIHGQLHDLLNLFTLNDTSIVGNEAINNSGDSNYKNGINGDGNDNENNNNNSNRSKAKQIPDVPNFHESIRNTRYIFLGDFVDRGFYSLETFLLLICLKIRYPDRIILVRGNHETRQITKVYGFYDEILRKYGNCNVWRYCCEVFDYIPLGCLINNGEIFCLHGGLSPNIQSLNEIKYLDRKQEVPHMGLMCDLLWSDPDENVDSWSLSPRGAGYLFGQNEVEKFTHLNGLSLIARAHQLVMEGYKEMFDKKLVTVWSAPNYCYRCGNVASVLQIDDNLNRSFKLFEAVPIKDNSGNAMIPNRKAGMDYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVT7</accession>
    <name>A0A1E5RVT7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein gar2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g518</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVT7">
        <property type="interactions" value="447"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVT7"/>
    <dbReference type="InParanoid" id="A0A1E5RVT7"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061574">
        <property type="term" value="C:ASAP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15481:SF0">
        <property type="entry name" value="LD23870P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15481">
        <property type="entry name" value="RIBONUCLEIC ACID BINDING PROTEIN S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="190"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="291"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="260"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="362"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="22"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="72"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="103"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="134"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="147"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="377"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="388"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="416"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ90843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="437" mass="47183" checksum="F5ABFC998932E0A0" modified="2017-01-18" version="1">MGVAKKQSKQSKKDLKTKEAAESSSSSSDSSXSDSDSSSSSSSESESSSSSSDSESSSSDSESESEEEEKVEEAKKEAKKESKKESKKETKKEAKKETKKETSSSSSDSSSSSSDSDSGSSSDSSSSSSSDSSSDSESESESEEVKEEVKEEVKEEVKESKKRKSDSESESQEEQEESSSKKPKLAGEPATLFVGRLSWNIDGEWLKSEFEHIGGVISARVIYERDNAERSRGYGYVDFEDISYAEKAVKEMQGKEIDGRPINVDMSTSKPAGGNNDRAKKFGDVPSEPSDTLFLGNLSFEANRDEIYEVFSKHGEIVSVRLPTHPETEQPKGFGYVQYGSIEAAQDALNTLQGHYINNRPVRLDFSTPKPRNDRGGNFGGRGGRGGFRGDSRGGFRGGRGDSRGGFGGRDRGKGANSTPVGRAPQTASFQGSKKTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVU4</accession>
    <name>A0A1E5RVU4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein transport protein sec71</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g287</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVU4">
        <property type="interactions" value="1099"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVU4"/>
    <dbReference type="InParanoid" id="A0A1E5RVU4"/>
    <dbReference type="OrthoDB" id="18431at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1000.11:FF:000003">
        <property type="entry name" value="Brefeldin A-inhibited guanine nucleotide-exchange protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032629">
        <property type="entry name" value="DCB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015403">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046455">
        <property type="entry name" value="Sec7/BIG1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF375">
        <property type="entry name" value="LD29171P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20252">
        <property type="entry name" value="BIG2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16213">
        <property type="entry name" value="DCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09324">
        <property type="entry name" value="Sec7-like_HDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SEC7" evidence="8">
        <location>
            <begin position="825"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="439"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="763"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1984"/>
            <end position="2006"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="65"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="114"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="160"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="443"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="763"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1984"/>
            <end position="2002"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ91014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="2033" mass="231642" checksum="269B6B32B018942F" modified="2017-01-18" version="1">MSEEHTVQKEQPELTRAELPEENHVEKSIDEQERGDEEQTQEQHKNEEGEENEANGENDIKEQAPNGNGVSKDTSNSSNNENETDYIINESPLNVDLADIDEAEDSXNVKAASEEEQEEEKEFQEEIPLENTXSLNESEQTSGQDSSSKQQKSEAFSEATENHQLDGSNEEPKDEXKEEPKEEXKEESSINGKPKPVNNNNIRSTLTFLKKTFQEICSHKEIKNSYSTTFKLLEKTIQEIDVSLATESEQNPINLDSIKIFEALRSSCRTNVTSAKVKSLDGLSKLFSFQLLDERVLVNPPDSLTTSAKISNNAGSNNTDQDPSSIVTPPPKLKLVDAAIDTITDCFEGEVTDQKVELQIIRCLTSCILMEDTLNXCHGQSLLKAIRTIYNIFIFSLNSSNQTIAQATLIQVVQKVFERVDQVSKKFAQLIEKKNQKDVANSDEEKVTDKQKSKNQEPLTLNALNSLNGQPEKQLESSAQEYYPELSAEENTQLGKFQLVIKDAFLVFRSMTKISVKNIDENMDIRSHAVRSKLLSLHILHSIIRDHVDLFINHDLRLISANGDEEIFIDSVRQYLCLSISRNSTSPITPVYEITLEIMWLLMANLRYDFKREIPVFLTEIYLPIADMKTSTTHQKRFFLNILNKLCNDPRMVIEFYLNYDCDSRFPNLLELIVDYLSRMTLTKCEITELEKKNYLDNLRKPLNIYDLSQLPLLSISNLSNSQLQASQTLNFPLEYACKMSSLQCVVALLRSLSTWAHKSLAPPTSTYSMSNTQNSIRNNSIDNDNDMDTLKESSEPSDAYSFDNTNRNRSSVVSLDGGEEILQQFENSKIRKNLLSKLIIIFNKKPKRAIPELIAKKFLADDTPESIAKFLLSTDGLDLATVGDYLGEADDKNIAIMHAFVEQMDFAKLSFIDALRFFLQKFRLPGEGQKIDRFMLKFAERFVEQNPDIFAKADTAYVLAYSVIMLNTDLHSPQIKHRMTLDEFLDNTSGINDGQDLPKEYVVGLYNEIEKNEIKLLSEQHDALLNDNNNNNGNSGSATGSATNSAFNFFNSRDLNREAYMQVSKEISTKTENVVKNLTVDEDDEASIFYVATHVEHVKFVFETLWMSFLASLTSPFKDNDDSFVVDLCLQGLQFSVRISATFGIEMARTSFVGAFIQFCNITNAQELKRKNVEAISKLFEVALLSGNFLKDSWKDVLLVVSQVERLQLIAKGIDKNSVPDVNNVRVVASNGRSSLDSTRSGAHSGNTKTSGGGFFNMSWASLGGSSASTNGGQNTVFDQTYEKHLGYKLKSNIAHLLVSGDLVILMDKIFSDSCNLSAQAIIDFIKALTQVCIEEIDSSLDAAQPRIFSLQKMIDVCYYNMNRIRVEWTPIWNVMGSTFIQIAANQKNLVVTFFAIDFLRQLSMRFLDIEELQGFEFQCDFLKPFVYILKNSFNNYQIQEMCLECFNQFIVIKGKSLKSGWKPILESLQYVSEHTTSEKLSVKTLDIINLGFTKKENFINLFNVENEKLDVFLEYIYVYKEICKQNKYPKISLRALNDLKKITVKIASQTFDAQKXDQDKDTFENGKEISEKLFDDVWYPILFTFNDIIMNSKDMEVRSCCLHEMFEFLFDYGSFFSITIWSKICNKILFPIFSVLKTSSNETVDKFNSHDDLSVWLNTTMIQALRNTIALFTHYYDKLEQLIQQFLNILVSCICQENDTIARIGRTCLQQLILENINRYDQAHWDLITKTFHQLFESTTATELFFDYGNGYQPNGNIDKSKTITKQDQNQDVKSTIVIKCILQLMVIELLSELFENENFVNYIPNDNVMELIKMLEKSYNFSKKFNDDYELRTKLVELRVVNKIPNLLKQESSSAAVLISCLFRLYFNNEDENVGDGKNTIAISSEDNNADLLEKLFDRLLDISLKIIQRYNSFDELRMEKNIITMTPVIIEILQGFNEFDNKEFAQICCFDGKIFYKEILNILSTKITATTTTLSNYSSSTVSIPNSTTNGNNTTTTATPPPTVDLRSSIKQFLERVGDLYIRDNVVET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RW43</accession>
    <name>A0A1E5RW43_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Suppressor protein STM1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g290</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RW43"/>
    <dbReference type="FunCoup" id="A0A1E5RW43">
        <property type="interactions" value="345"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RW43"/>
    <dbReference type="InParanoid" id="A0A1E5RW43"/>
    <dbReference type="OrthoDB" id="5426471at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006861">
        <property type="entry name" value="HABP4_PAIRBP1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019084">
        <property type="entry name" value="STM1-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09598">
        <property type="entry name" value="Stm1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01233">
        <property type="entry name" value="HABP4_PAI-RBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hyaluronan/mRNA-binding protein" evidence="4">
        <location>
            <begin position="84"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="86"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="129"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="164"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="182"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="233"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ91015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="290" mass="32348" checksum="F47DF65843D5C6DC" modified="2017-01-18" version="1">MSNPFDLLGNDVEDSTSEILVTKELTRNSGSSKKSDVNPKADKSKAKKRTPSPNQANENALKTKNINNKIDPAATANQKQRSNNKSKFDKHNKSGKTDSSKKVKKGWGDDKKEGEFEKEAEQDAKLDETVDAVETLESEEATSTEPKEPTVPKKSLAEYLAEQQKAQNALNSKNATKASVESVEHATKLEKDLIEDTVGSNKKQPRSKNLKTKEYLEFDITFQDSVPTGARDNNNRQRREGNNNRERKPFGGKDNRQRKEGNNKNTKPENKQGANVAQFAKKELNFPSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RW53</accession>
    <name>A0A1E5RW53_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g432</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RW53"/>
    <dbReference type="OrthoDB" id="5563016at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="450"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="571"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="668"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="721"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="828"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="468"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="491"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="545"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="585"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="626"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="668"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="692"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="725"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="829"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="863"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="890"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ91025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="970" mass="107393" checksum="88EAFC91F6BF2973" modified="2017-01-18" version="1">MYQNEARSNSMSSLSSYHSAKTVHIPRSDSVTDLFTLQKQLNNKKSQDMKHRNKRTSSLSMGSLANVITDRNKIQNKYQSNMLQQQQNVPHSPHSPKRRDYNRSALHSKTMQQQQQYSSTLLETPNQYSTTLLETPHQYSSTLLETPHQYSKTPPPFAQHRGQKKPLLVKQKTVNTASLQSKPLLEKSLYPVQTNNSFGALSHKGSNSTLNKYKQNHSRVGSSYTLRQYAAHNANGSSANGNTSQTNIVSPLAQPMSHXQTMVIPQHQQSLXMPHQQSLXMPHQQSLSMPQHQSLNTPPPRLHPFTAGGSVSKPVQFSNSPVPQYRGNNSYTPTQQSMQKTVPAGVHRPVNNYMQAAPQAYVAXPGNKATYWNAPPQNIEQFTGNHQVNGNVPVGRPRMLVYTGESNKIRKPGLSKRQLLKMQMRTAFQFPNGEKFTPRTVQNVVDLEEKEQPQQQDGDLHNGVSDXSHANNGISSNAHFNAPXSAVPQPKNDKAEDSQSHPDMKKSXSERTSFASSNRTITGPKLEPDTKNDEVLNXTPGTATSKSKPVPVSPVKKQSKFSKFFKNIFSSGSSSKPLPKPEPSLQEKRSKSTKDLTPIEIKPQSNTNMETNDDSKTNKPIINLSTQNPLPATPTNPLTDMTLESPPVSPLIDIDIDMLFDSLMISSSSPEPFSTTXXSASNGSPEKPPLFSHSQNDMGSRTSSITSKSNMARLKLLQQANMSNSSLTSSKKTSSPLKSPRGIDEMMQGSGSDLXENHDNTSEGASSIHSMSDGLXGNEAYEYEDYPVFIERLGQHFSSIKTLNDDGSCKVYDSHGFIVKQDSIVSLPTTKSSNSTYKSNLHRKDGSPKILKHKGCMHSANHSGSTNNSSLSSASSSSRANSMSTKSTSPMANSNSSQTQQQMLKFNNTVEVFPVYNSEEYERNFDLSFKNATRDHLMKNIGAIKVELNQYKKFQMLVHHDSVENTHLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RW66</accession>
    <name>A0A1E5RW66_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fimbrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g364</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RW66"/>
    <dbReference type="FunCoup" id="A0A1E5RW66">
        <property type="interactions" value="609"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RW66"/>
    <dbReference type="InParanoid" id="A0A1E5RW66"/>
    <dbReference type="OrthoDB" id="431378at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032432">
        <property type="term" value="C:actin filament bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051639">
        <property type="term" value="P:actin filament network formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110009">
        <property type="term" value="P:formin-nucleated actin cable organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21294">
        <property type="entry name" value="CH_FIMB_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21300">
        <property type="entry name" value="CH_FIMB_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21303">
        <property type="entry name" value="CH_FIMB_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000042">
        <property type="entry name" value="Fimbrin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000010">
        <property type="entry name" value="Plastin-3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000016">
        <property type="entry name" value="Probable fimbrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000027">
        <property type="entry name" value="Probable fimbrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039959">
        <property type="entry name" value="Fimbrin/Plastin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961:SF18">
        <property type="entry name" value="FI19014P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961">
        <property type="entry name" value="FIMBRIN/PLASTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="6">
        <location>
            <begin position="148"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="6">
        <location>
            <begin position="296"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="6">
        <location>
            <begin position="420"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="6">
        <location>
            <begin position="542"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00073963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ90998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="650" mass="72301" checksum="6A33DF58DC026F3C" modified="2017-01-18" version="1">MNIVKLQRKFPILTQEDLFSTIERFRSLDIDDKGWIAKPEALESVSASGEATYDQARQTLKEVEVDASGHVELDDYVELVAKLRDSNQGGGASSSNAAPKTSFPGLNTTERIAVSSSNPSMLQLKGTGAQARIVVGGSQSGTTHTINEEERSEFTKHINSVLAGDSDIGDRLPFPTDTFQLFDECRDGLVLSKLINDSVPDTIDTRVLNWPKNGKSLNNFTAGENANIVINSAKAIGCVVVNVHPEDIIEGKEHLILGLIWQIIRKGLLNKIDIQLHPELYRLLEDDETLEEFLRLAPEKILIRWFNYHLKQAGWERRVTNFSSDIKDGENYTILLNQLAPELCSKQPLQTQDLLTRAEQVLENAEKLDCRKYLTAKALVAGNPKLNLAFVAHLFNTHPGLEPIAEEEKPEIEEFDAEGEREARVFTLWLNSLDVDPPIVTLFEDLKNGLALLQAYEKVMPGVVDKRRINNKPANGNELSRFKALENNNYVVDLGKENKFSLVGIEGADIVDGNKLLTLGLVWQLMRRNIDLTMQHLSKGKTLSDAEILSWAHAQVAKGGKSSKIRNFSDPSLSNAHFLLDVLNGLAPGYVDYDLVTPGTTDEDRYANARLAISIARKLGALIWLVPEDINEVRPRLILTFVASLMALNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RYV5</accession>
    <name>A0A1E5RYV5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Arginine metabolism regulation protein II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g245</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000002">
        <property type="protein sequence ID" value="OEJ92162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RYV5">
        <property type="interactions" value="291"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RYV5"/>
    <dbReference type="InParanoid" id="A0A1E5RYV5"/>
    <dbReference type="OrthoDB" id="3972764at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF32">
        <property type="entry name" value="ARGININE METABOLISM REGULATION PROTEIN II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="114"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="387"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="452"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="827"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="67"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="461"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ92162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1090" mass="124390" checksum="368F8C3CC712AB2E" modified="2017-01-18" version="1">MMITNTQGDFQDNAGTGLTNPQLLHTVLTTPGIKSREPPQKLHGKTKISSSGASQRRNTQQLRSDCSDDELDDEEDFDLDEDDEEEDEDMDDGNRNGYLLQGSADKKKNMSFTGCFTCRKRKVLCDKTRPRCIRCTKSKFQCDGFDIKLRWRSSIKFNTYGEIISNPKNSKLLDSNNQEPTFQRRKIKYLHYKEEYQYYDEMDNELTQLNKVLKENKEKLDNNQTWVIKKFGVMWGKNDLREKFLKKFEKKERKRTFNGNIDDSKPKKLSRKHSQEAQNLSLNKHNLLDADQGMESAAQQKLLDDSLNFNFHFNNPLGTEWISEELREDAIISATAVASSQVNSFLSSFKNMYPSASTDYLQGXQNQLQQTNTSLLPSFSPFVSNTPVPINNETIQGPNQNNDTEFVSRNMGPSIQEYNQHRNQNTVNSKNNNSSSFDFALDEQHIQNNLSTTKTSLGNDNNNNNSSSINNNNNINNINNNNNSSSNIFSILFHTNDNASMQNSQSQLNIPFQNSVTQKPPNSSTQNITNKIVPFGNSIAIQAPSTEAEMPSVVLKIVPSEIPEEVRQEMCAYSQQSEDNLLKDIPTTGLMTTGFLRFLLNYYLEKVANMMTVIPLPNNPWKHIYLPRALKGLGDLVGLGYTSNSRNCLLNALMAVSCFNIQSEFPKESTTKAFFLQLGIKFRAQAVSFLDSCLNSTVEQEKYKDVLTALLSMNTIDVIWGTMGDCSRHLDICEAFILKRIQERPKISEKAKSLHKVFSFLRLIQNSTALENITADEIAITDDSLKTGNEPSRRASVPATAMDRMSRLQSINHMNPKSHQLIKTYSSNTTQSPKNDRLSSCGSTPTNSVSTNGPITPTPLFQDFKTALYSNTANSTDTRXKPINYDIDDMYGLPNSLVLLFSDCVNLVRHKIYYEANPDVAIPQTFEQLCKDFEKKLFKWRSEWTFFKPDAPQEFLDDIVEALYYHQNIFYNAIIIYYFTIVRNVNCSILEKYCALVLESCVLLLRSIKDKKVPIVPVIWGAFIGGCAATEEKQHQYKQWFQDLADSNKFSKYWLSRQIMMEVFRRRDNGENNDNWVSVVKEWGVNIMLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZD1</accession>
    <name>A0A1E5RZD1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycine cleavage system H protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g224</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">The H protein shuttles the methylamine group of glycine from the P protein to the T protein.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="5">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GcvH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZD1"/>
    <dbReference type="FunCoup" id="A0A1E5RZD1">
        <property type="interactions" value="895"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZD1"/>
    <dbReference type="InParanoid" id="A0A1E5RZD1"/>
    <dbReference type="OrthoDB" id="10264154at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06848">
        <property type="entry name" value="GCS_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00272">
        <property type="entry name" value="GcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002930">
        <property type="entry name" value="GCV_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033753">
        <property type="entry name" value="GCV_H/Fam206"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017453">
        <property type="entry name" value="GCV_H_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00527">
        <property type="entry name" value="gcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002270">
        <property type="entry name" value="PRK01202.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715:SF3">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01597">
        <property type="entry name" value="GCV_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 5" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="6">
        <location>
            <begin position="64"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-lipoyllysine" evidence="4">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR617453-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ92332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="171" mass="19181" checksum="E429B93C50D59224" modified="2017-01-18" version="1">MLRLQSPFLKNTVSLFQRSVRFNSTTIGNGLTKTELPYTFTASGPIQVKYTPQHEWLAIHPNNIAFIGITRYAAVALGDATYIELPEVDSSLEIGESFGSVESVKSASEIYSPVKGTVLDVNGELQDKPQMINHDPMGQGWLVKVNFEELEREDHLLDLERYEASLKNDTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZG7</accession>
    <name>A0A1E5RZG7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Inorganic phosphate transporter PHO84</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g263</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000002">
        <property type="protein sequence ID" value="OEJ92155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZG7"/>
    <dbReference type="FunCoup" id="A0A1E5RZG7">
        <property type="interactions" value="867"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZG7"/>
    <dbReference type="InParanoid" id="A0A1E5RZG7"/>
    <dbReference type="OrthoDB" id="433512at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17364">
        <property type="entry name" value="MFS_PhT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="249"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="345"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="394"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="424"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="520"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="68"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ92155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="577" mass="63271" checksum="50F1ACEF30895154" modified="2017-01-18" version="1">MKDEQIKVNTNPMEKQDEQNVYMRTDGGNAAFHNFINDFSHIKDPLERRRLALEKIDNAKFGWYHIRAILVAGVGFLTDSYDIFAINLAITMMTPVFFPKGLTYGMETFLKVSTSVGAVIGQVGFGILGDVLGRKGVYGFELIIVIFATIVQCCLGVAAGLNFGFLLGFWRIIMGVGIGGDYPLSSVITSEFATTRWRGAIMSAVFANQAWGQLAGGILAVILVEAYKNEIDVPKAQCEHKCLKALDQMWRILVGFGAVPGCVALYFRLTIPESPRYTLDCENQINQGAAEVSRYLAGELGDAENDEMNVLNRTGTQMDAIDTHQPKASWKAFWGHFGQWKYGKILFGTAFSWFFLDVAFYGLGLNTSSILKAINYTKKGTLYESLSTTAKGNLILLAAGSLPGYWFSIALIDTYMGRRSIQLMGFVLLTVIFAVLSGCASKNANVTDKFLFFLYVMAEFFCNFGPNTTTFVTPGEIFPTAYKSTAHGLSAGWGKVGAIFSQCVIGKFLGGEENGVTKAMGVYAGIMVLGIFTTLLVPETKGMTLEEISVKYHDTVDPNLFIEQSSTGTETDEEAQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZL5</accession>
    <name>A0A1E5RZL5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Inactive deaminase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g22</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZL5">
        <property type="interactions" value="97"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZL5"/>
    <dbReference type="InParanoid" id="A0A1E5RZL5"/>
    <dbReference type="OrthoDB" id="1723809at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003876">
        <property type="term" value="F:AMP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006329">
        <property type="entry name" value="AMPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359">
        <property type="entry name" value="AMP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359:SF7">
        <property type="entry name" value="INACTIVE DEAMINASE YBR284W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19326">
        <property type="entry name" value="AMP_deaminase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="843" mass="98606" checksum="B3DD198084D0D50A" modified="2017-01-18" version="1">MTNLXNNTQSEKVQWKESLDNENECDFDQVYHYSDNSNEHPLLERRPSMLYSENGEEGEEDAYDEDSKFHIRPSELGEEYSRGNQLSKTQGMFHPHNEFMHHFNTVDKFKPHPIYSNNQKYKDQHKHPLETLKEFKIKSAFAAKSLDDLDMVGLSTDFDKEMYKGYLDEDVEQELQKLIKMILNLKDKYNGDDNTSSINNLTSRVDPNSAALPRIESKSNGIHNPEIKNITEFIRDYDRISECINDFCQGYYKTYVTKKNSYLLNKFELYTNLCSNLEIQQTKMIPHKDFYNVLKVDPDLLINGCFSIHQLNNFIMNKLRTDSKKIVVELDGQTKTLGELFETGDSNTSSYEHVVIDDDFLEWYQYQYLPNHSGSLEKVMFLNDQDMDANTATKLFYFKVAKLFLEFDNYIEGQYLFELIETCIFKRKSRHQLYQLSIDFQFNMAKDEKSXKLYNQWCENFYXFNERFAKHNHRNIEWNVRVHRNYTYLFEQGLVFNFEDYLNEIHHLIKNAPKVNCSIKHIDVIYRDSDSYLWKSFSCTAATSPANWNISDNPPLAYYMFYIHQLLSENTIKSKGNXVCDNNGNLCSNITLRNNCGSQKSMGKLSQFTMLGDSIYKESLIANILLADSNQITSAEPIAQTAIFQYLYYILQITCIVSPLSTVSKYYGGSDVHDYNKSNDTLYHATNFSTASINKTPQSNSHSSSDVSMKNRDLNYSRASNFFYRGNPFMDMHKMGIRVVLSSHSIVFNNSYTRDPLLEEYSVAASIYLINSCDMCELALNSTTDHGDYNYKDETYDHPFEDNEEEEAYGYEDDVYKHNVAPVRRQYRITTWQTEMNNLKLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZP4</accession>
    <name>A0A1E5RZP4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">diphthine methyl ester synthase</fullName>
            <ecNumber evidence="4">2.1.1.314</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g156</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 4 S-adenosyl-L-methionine = diphthine methyl ester-[translation elongation factor 2] + 4 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42652"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10173"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="ChEBI" id="CHEBI:79005"/>
            <dbReference type="EC" id="2.1.1.314"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the diphthine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.314" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZP4"/>
    <dbReference type="FunCoup" id="A0A1E5RZP4">
        <property type="interactions" value="953"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZP4"/>
    <dbReference type="InParanoid" id="A0A1E5RZP4"/>
    <dbReference type="OrthoDB" id="2516at2759"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141133">
        <property type="term" value="F:diphthine methyl ester synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11647">
        <property type="entry name" value="DHP5_DphB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.950.10:FF:000004">
        <property type="entry name" value="Diphthine synthase putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1010.10:FF:000004">
        <property type="entry name" value="Putative diphthine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01084">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004551">
        <property type="entry name" value="Dphthn_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00522">
        <property type="entry name" value="dph5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882:SF0">
        <property type="entry name" value="DIPHTHINE METHYL ESTER SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882">
        <property type="entry name" value="DIPHTHINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036432">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="10">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="113"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR036432-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ92306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="299" mass="34327" checksum="9A2A64C53986785B" modified="2017-01-18" version="1">MLYLIGLGLAYPEDITVRGLRAVQKCERVYLEHYTSILMNSSMEELEEFYGKKITLADREMVESGCEEIMENADKVDVCFLVVGDVFGATTHTDLVLRCKQQNIPVEVIHNASVMNAAGACGLQLYNFGQTISMVFFTDSWRPDSWYDKIMENRKIGLHTLVLLDIKVKEQSIENMARGRLIYEPPRYMSIAQCCEQLLEIEETRQEKAYTPNTPCVAISRLGSNKQAFKAGTIEQLSKYDSGEPLHTLVILGRQTHELELDYILEFVDDKENFKKMVIEDQEFFKPAPWVPEEEHMSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZQ5</accession>
    <name>A0A1E5RZQ5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">External alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g175</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZQ5"/>
    <dbReference type="STRING" id="56408.A0A1E5RZQ5"/>
    <dbReference type="InParanoid" id="A0A1E5RZQ5"/>
    <dbReference type="OrthoDB" id="5376590at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045024">
        <property type="entry name" value="NDH-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054585">
        <property type="entry name" value="NDH2-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706:SF50">
        <property type="entry name" value="NADH DEHYDROGENASE (UBIQUINONE)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22366">
        <property type="entry name" value="NDH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0830">Ubiquinone</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="7">
        <location>
            <begin position="135"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="External alternative NADH-ubiquinone oxidoreductase-like C-terminal" evidence="8">
        <location>
            <begin position="619"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ92447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="684" mass="76435" checksum="2B2F8996060EBD1E" modified="2017-01-18" version="1">MIGRSFRGMQSIPKMCTKQCSSRPSQFLSFPHTRKQLLHTSPVYRNSLKRSAVKVAKYGIFATGLLVSSAALSMIGFFIYDATTYKTDTKCDTIQVPQLAISPETGGPENLPILSTSLDSLDTNDKAVIAVNKPKLVILGSGWASVGILKSLRPGDYDVTVISPTNYFLFTPLLPSAATSTLEVKSLMASIRSIVKKISGHYLEASAEKIEFADKLVKVTSNYDTKDSFYVPYDKLVIAVGCTANTHGVEGLEHCERLKYARDVIAIRKKIKDNLERACLPTTSDQERQQLLSFVICGGGATGTEFAAEISDLLNEDLPEQYPKILRQEASVHIIQSRSNILNTYDERISEYATKKFLKDDIDVQINSRVEKILPDKVIFKQMNAETGEYDMKELPFGLCLWSTGIAQNPLAHSVVETLGPYFQKNKRAIETDSHLRVIGCKDVYAVGDCSTVRTDLADYAVEEVKNFIIHKHHPNAKWSNHKDFPDEEIEKMSLTYEDICQFGTGLMKKFPQAREHLITLEEVLPEYDINNTGQLSYSDTVKLLKDVESKVTTLPATAQRAHQQGKYLGKNFSKLSKKMEALDKESENTIASGTTLVPTTTTFPLSDEEYAKPFKYVHLGSLAYIGNSAIFDMPGYSFVGGIVAMYLWRGIYFAQTVSLRTRLLLCMDWMKRGLFGRSIVNED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZZ6</accession>
    <name>A0A1E5RZZ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Myb-like DNA-binding protein myb-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g23</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZZ6"/>
    <dbReference type="InParanoid" id="A0A1E5RZZ6"/>
    <dbReference type="OrthoDB" id="3972899at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050891">
        <property type="term" value="P:multicellular organismal-level water homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901002">
        <property type="term" value="P:positive regulation of response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902584">
        <property type="term" value="P:positive regulation of response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902806">
        <property type="term" value="P:regulation of cell cycle G1/S phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032875">
        <property type="term" value="P:regulation of DNA endoreduplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000037">
        <property type="term" value="P:regulation of stomatal complex patterning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033993">
        <property type="term" value="P:response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000355">
        <property type="entry name" value="Transcription factor MYB124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF25">
        <property type="entry name" value="MYB PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="109"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="109"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="161"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="165"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="42"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="227"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="475"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="525"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="567"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="720"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="42"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="235"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="476"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="530"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="568"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="591"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="720"/>
            <end position="737"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ92325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="801" mass="86796" checksum="455279EB5DD66DE5" modified="2017-01-18" version="1">MNMKKELDQAGPVMSNGVSVHGLMDNNNMNNMNNMSNMNNMSNMSNMSNMSNMNNMNMSNMNNNSMNAMNSMNNNNNNNNNNSMNNNSNESTTYNPDSTQHDGSSASQGMMIKRGPWTEDEDEMLLQYVDYFGISNWVRIASYLKSRTPKQCRERYHQNLKPSLNKTPISEEEGIRIEELVAHIGKKWAEIARIIGNGRSDNTIKNWWNGGQNKRKRKTLREQQHLKNARLVNGTPPTSGVNTTNKVSPHHVPQPRLLQQQQPPPPPPSGFQQQVYQRPYMAQPHHQLPPLVPATGPHYTTGLQDPVISRKNSYTFEYASNNSLDPSRRSSVSYLDSYHQVAGNANANANANANANANVNVNANNTTIPSSTSNVPVNSTTNGPLFKRGSVALPLPANPMTPYSMSSPGSPNPMNIPSLNSTTNSLASGNSMTSLNTISNGTGANTPSLLNPQAHMNYFTSPMYANSHRGSIISLNNNNNNNNNNNNTSVTTQQPGTDFVEHHPKLTKSNSTNNLMKWNFKFNNGNSVNTAPPQASSTAFPPSTTQSTTQSNADGIFKQHYHMAPNAPLSSLSAQPSNTTASSPLAHVQLNPQSNPQSNPQSNPHSNSNSVASATTTTTTAPSITSSDQHMMMALGRRGSSISMLLNSSASSKANLTPITSNPSSTPLEEMSPEVQLKRRLSKQLLPVYSHDISHHYPAQXLPGRRDSWGLGPNTATASTPVAGNAATPVSFNTSPVDTGKKFNPPSGLKLNYSMSFDAPNIGSQCKTGSSIVLPPIHNIGATTANNNSNPGPAYQGHLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S0C5</accession>
    <name>A0A1E5S0C5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transgelin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g234</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5S0C5"/>
    <dbReference type="FunCoup" id="A0A1E5S0C5">
        <property type="interactions" value="835"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S0C5"/>
    <dbReference type="InParanoid" id="A0A1E5S0C5"/>
    <dbReference type="OrthoDB" id="21595at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050606">
        <property type="entry name" value="Calponin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003096">
        <property type="entry name" value="SM22_calponin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385">
        <property type="entry name" value="CALPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385:SF14">
        <property type="entry name" value="TRANSGELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00888">
        <property type="entry name" value="SM22CALPONIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="1">
        <location>
            <begin position="29"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="210" mass="23556" checksum="51FB5DBA347D0641" modified="2017-01-18" version="1">MKDFGHPGKADVTSLDEDLLNKRSAKFTQENINEIQNWIFNDILHKVPESQDQDLIDLLKDGCVLCELANVLSAGSDSVDGSEAPLIKYKKSAMPFVQMEQISLFLQFAQQYGVPQDELFSTVDLYENKDPAQVYQTLKSLSRYANAKNPANFPVLGPQLAQKRQAPLKPKKPSHLTNASWSTMEFGYMKGANQKTEKISIGTRRNITSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S0H3</accession>
    <name>A0A1E5S0H3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oleate-activated transcription factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g231</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5S0H3"/>
    <dbReference type="FunCoup" id="A0A1E5S0H3">
        <property type="interactions" value="1345"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S0H3"/>
    <dbReference type="InParanoid" id="A0A1E5S0H3"/>
    <dbReference type="OrthoDB" id="5069333at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF29">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="247"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="120"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="212"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="339"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="140"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="339"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="363"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ92473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1164" mass="131656" checksum="A29C7F56A940EE62" modified="2017-01-18" version="1">MVNNNTNNNNSNDMQNPSGYNYPDPQNRASFTTNRNLHHQSLYVLPNEQDQQQWNANQRVNFNRSNVHSLLNESTLPHNNMPNTQRAQLQSQPMLPPVNPQNGHGNHVIQTYSQPNLMMNLNNYNSGQPNILHHYPDGLSSSSTSSNTNSSQNNAEKIQDGLSGEATSTSAKPHGDSSAENLAGKNHLSVANLLSANSEANVYYDNALSSSNSSELAEDGASQSEKTADSDKVSKQGTKKRNKLSFNCVNCRKRKSKCDRLKPSCSKCLQLGTSCFYDTDTQVGPKRPNKDAMIARLSKQVEYWKNKALKENSTGPAFHHDQNNENNRLYNNSTALPLDLQNNQSHSHDVSTSPPLGADGVASGSSMPNGHTRDNTTGNTSTYQPSESDLSTLTINPEHIYIDFKTQAPSFMRSNGVTKHFDIFSDIAAVQKDVFLRVFMSSIMGFSSTKNEKQTSQEKKQTIAKILDSTVYSEYTLSEVERASCEHYAQKIAFGKNDEQQGTANLQYALLSGLIDVKSVYIEDSISPGEEQSPLLKQSLEMMKSVLPPKKTIMQYKSFFYSKIYSSLSALNIETFEKSLATSLVDDRENPEFFKINVGTSGIREKMENLVLLCVIMKLSDVALQQILLKNSVMGNTSGPAVQFAPNEDHIKRFSSIIDVSIKILTFLNAMAWTTENTLCCLMYMWSYLVVSPGEADMFSGKPTDLLANLIVTLACNIGVDVDPLDIVYFQKNKKSPLINYRRVLNVSLSTILVIEDLFKGKMYLNTAVHEQVLSVKHQQFLDKMLETRPEMSHLDKKILSLNFKRAQLYAIICEFYHNSRKDKIVSLFSVETNMLKINGFLEKNFLLSNLKKPSSNSEEVLTGTNTKISSTLVENTYCIELVFFAKAHTLMVNNSVFISLQNSSKETKSSKNYFPFYKKYLIVLIHDVCSSFKLLEDFFEGKYTYALNNRESLSMLKSVVILMNRVIFIILALLVRFSFTKQILLKKLSNRLENVFNSKEIQLRIELITKMSDQTTQLLKRLCNYSTRKLRFSYFQSFKTSMFFDYVKILTERNELCNILLKILDKDVQKEKLTPKAVDLLQNTMGFDVNHSDDFKEDLELSELLSFVPLSLLEEYLNTFMVTEVLETEEDESKGKSRSQTVSIPFDVDELNIFDYDFLFKGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R004</accession>
    <name>A0A1E5R004_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Low temperature requirement protein A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4660</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000017">
        <property type="protein sequence ID" value="OEJ80235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R004"/>
    <dbReference type="STRING" id="56408.A0A1E5R004"/>
    <dbReference type="InParanoid" id="A0A1E5R004"/>
    <dbReference type="OrthoDB" id="191995at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010640">
        <property type="entry name" value="Low_temperature_requirement_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36840">
        <property type="entry name" value="BLL5714 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36840:SF1">
        <property type="entry name" value="BLL5714 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06772">
        <property type="entry name" value="LtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="348"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="399"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="438"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="486"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="499"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="526"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="61245" checksum="578A8BB3F248AAF9" modified="2017-01-18" version="1">MRNDIENLSMKSSRDVEKEEQKAKSDDGEEKQQQQQQDSYANKYGPAKCKWIDAPESAKRGNLFIKPYALNYFHDGVLYRTQESRGSTIFEMFFDLLYVGIVANLAQGCISESNGISLARDILLFLPCWQIWGDMRDFMDYYYNNDMIQKTYVLWIMFLMVTYANNAATVVQNDKALTGLVVACYMLARFSFATIVLVYNVFFVKEHRKQMLWYCAFVYGSVIMAGFVILPTRMYQKIIIVCCLYFWDNLSYSISFSAWFKKLIRAEFYAAMNIEHEIQRHNSFVTIAIGEFLYPIVAYAPASGGLNETTARCTCVLVIAYCLTWFYFAGEGSRKAIHAIRRHSITGLCWIQFHLPLIISLQLAANGAGVLTTSKVDHPNSITDPSALGMPRKNYLQDVQIYFGAGLAVSLTVLTCLALLDKGLDDKRFWIITPPMRILPRILWGLAIFGMSFAKMKITLYMGLSALFLTIQLIFENIVEAKSFSRNKEEEQNNQASKGRPEDEQDKDLLAKQQGNKAQEFENEESDYKDSEKSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R092</accession>
    <name>A0A1E5R092_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g4414</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R092"/>
    <dbReference type="FunCoup" id="A0A1E5R092">
        <property type="interactions" value="1295"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R092"/>
    <dbReference type="InParanoid" id="A0A1E5R092"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17982">
        <property type="entry name" value="DEXHc_DHX37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000023">
        <property type="entry name" value="ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003770">
        <property type="entry name" value="ATP-dependent RNA helicase DHR1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000637">
        <property type="entry name" value="ATP-dependent RNA helicase DHX37/DHR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF99">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX37-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 14" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="413"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="694"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="27"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="167"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="697"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="221"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="241"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="257"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="301"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="697"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ80319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1263" mass="144042" checksum="57BEB02AA19B3EE6" modified="2017-01-18" version="1">MGTYRKRFNEKARAGHMAKVKDLKKVRNKQFYRHMDDGTEQSDHSDAADDSNKEVLLPMSKQEKEERKRKMHDEIFADSKQESKVSRQKRKRLDKFIDHQLKREEKKKILEEMQNYQMDTSKLTSIKTIGQGKQTKKEQFQEALALERDGKTLTEDQKDVLYEKKEQKVWDKDDQSSVSENDNNDDSEDNFYSQFGDTEQSSGSFIDNRPQNGGGFGFGFSNATVVKNKQTGPLKKYNWRQKVEIEEAKRNKKDDAMDFDSSSEDFSSDEDDDSESSDGSEIFEEDGESVDGSFNGFSNDESTDAGGDEEEKDQSDVEVFQNVKDENNSDFKSWAKQEIKKMEGNDVQYVIPTNVHVERTVREEDLEDGLKEEHVPINESLSRKAYSVSIERDPEIQSTRMKLPVFGEEHRIMEAIHHNDVVILCGETGSGKTTQVPQFLFESGYGTAPETPGLIGITQPRRVAAVSMAKRVGNEMGNHQGKVAHQIRFDTTVDESTRMKFMTDGVLLREMMTDFKLSKYSAVIIDEAHERNINTDILIGMLSRCVKLRAKEHGENPDKVKKLKLIIMSATLRVSDFSENPVLFPVPPPILKVEARQYPVSVHFSRRTAYDYNEEAFKKACKIHQRLPPGAILIFLTGQKEITDMVKKLRKEFPFPSSKPYKNNGKLISMKVDSQNTDVEVEDINFSVKINKDEDKDYYDDDLVGENDSDEDEEEDGFEETLEEGQTPNDPLYVLPLYSLLPTKEQMKVFENPPPGSRLCVVATNVAETSLTIPNVRYVVDCGRSKERKFNEETGVQSFEVDWISKASADQRSGRAGRTGPGHCYRLYSSAVFDRDFDQFSKPEILRMPVESIVLAMKSMAIHNIVNFPFPTPPDRKSLFNAVSLLKYLGALDTDEKITADGKKMSLFPLSPRFSKMLLVGNEHDCLAYVVAIVSALSVGNPFIDEHELGLSNTVMETNEDGELVKKDAVFDEEKKQMRTKFYKSRAKFSKLDKFSDVFRLLSVVSAFDYIPSENRDSFLNNNFLRKKLMLEIAKLRKQVMYIIKLHTSKENIAVSVDDNELKTRLPTETQIKMLKQMVCTGFVDQVAVRADYLFPEEFKLSSKSSVINIPYIPVLAKKDTLKEISDLCVYLHPTSILSNVGEAPPRFLVYQSLQQNMNTMKTRMKPLCDIKSTPLVNIARKGSLLTYSKPLTGQGLKPVDISLTERYCYVVPRFGASVDSDLKIGWDLNPVAVAQKKINGKWHVERIITNKTYKDLEQQKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0F5</accession>
    <name>A0A1E5R0F5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycerol-3-phosphate dehydrogenase [NAD(+)]</fullName>
            <ecNumber evidence="9">1.1.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g4477</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 9">
            <text>sn-glycerol 3-phosphate + NAD(+) = dihydroxyacetone phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.8" evidence="9"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0F5">
        <property type="interactions" value="623"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0F5"/>
    <dbReference type="InParanoid" id="A0A1E5R0F5"/>
    <dbReference type="OrthoDB" id="10263760at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141152">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046168">
        <property type="term" value="P:glycerol-3-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000004">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006168">
        <property type="entry name" value="G3P_DH_NAD-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006109">
        <property type="entry name" value="G3P_DH_NAD-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017751">
        <property type="entry name" value="G3P_DH_NAD-dep_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011128">
        <property type="entry name" value="G3P_DH_NAD-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03376">
        <property type="entry name" value="glycerol3P_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11728">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11728:SF8">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE [NAD(+)]-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07479">
        <property type="entry name" value="NAD_Gly3P_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01210">
        <property type="entry name" value="NAD_Gly3P_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000114">
        <property type="entry name" value="Glycerol-3-P_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00077">
        <property type="entry name" value="GPDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00957">
        <property type="entry name" value="NAD_G3PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0520">NAD</keyword>
    <keyword evidence="2 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycerol-3-phosphate dehydrogenase NAD-dependent N-terminal" evidence="10">
        <location>
            <begin position="21"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Glycerol-3-phosphate dehydrogenase NAD-dependent C-terminal" evidence="11">
        <location>
            <begin position="229"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="25"/>
            <end position="30"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="305"/>
            <end position="306"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ80382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="403" mass="44501" checksum="DCDDE1B6295C3C91" modified="2017-01-18" version="1">MSERLNQVSNIVEESATKYNVCVVGSGNWGTTIAKIASENCLQYSDSEFNELVKMWVYEEEIDGQKLTEIINTKHQNVKYLPNIDLPHNLVASPDLIQTLENADIVIFNIPHQFLGNIVKQVLAHCEKENIKFKGGLVNKDCIGISCLKGFHLDPTKGVTLLNDYVTETLDIPCGALSGANLAPEVAKEKWSETTVAFDTHNNPNXXIDHNTLKKLFHRPYFHVNVIDDVAGVSVAGALKNVVAVGCGIVLGLGWGDNASAAIQRIGLSEIIAFGKKYFPECSVETYYKESAGVADLITTCSGGRNVRVATAFAKENAYAMKKKLPTKSVFDIEKEILNGQSAQGIITCKETHEWLVHDKSQDEFPLFEAVYHIIYEGANMKKLPEMIEDLQGLEVPESEDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0K7</accession>
    <name>A0A1E5R0K7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Monothiol glutaredoxin-6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4454</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0K7"/>
    <dbReference type="InParanoid" id="A0A1E5R0K7"/>
    <dbReference type="OrthoDB" id="423313at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005796">
        <property type="term" value="C:Golgi lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015038">
        <property type="term" value="F:glutathione disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03419">
        <property type="entry name" value="GRX_GRXh_1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014025">
        <property type="entry name" value="Glutaredoxin_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694">
        <property type="entry name" value="GLUTAREDOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694:SF5">
        <property type="entry name" value="GLUTAREDOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00160">
        <property type="entry name" value="GLUTAREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="2">
        <location>
            <begin position="250"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="357" mass="39393" checksum="BD2DA49A256EF413" modified="2017-01-18" version="1">MSNNTQSTSSLIDSSSAAMSNLYSGVTKEGDSSSXNAGNDGDNSPIIDHTDXVKPQYKDSETIGITLEDLRLEDEEEVEIPVDIPYMKASGQTKQDEDLKSVDMTTSNDITEQTKEQQQQQEITQQQQSQEMTQQQQSQEMTQQQQSQEITQQQEKLQGSSQDTEPLHAGTIEHTSSVISPVETSTASVSTLSPNPTGTSQESQVSQELDTTFGTEVIKGDNVFDNSEGDAQTAYDAAKDYKSIMDQSSIVIFSKTYCPFSQALKDLLLSEKSIYKFDPEPVVIEFDTMENKNGPILQEYVKEQTGRKTVPNLIVNGISRGGSDDIHALHEKDTLYESFEEWCGTLCKISKVLAPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0T2</accession>
    <name>A0A1E5R0T2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein SOK1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4504</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TCP11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0T2">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0T2"/>
    <dbReference type="InParanoid" id="A0A1E5R0T2"/>
    <dbReference type="OrthoDB" id="276323at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010737">
        <property type="term" value="P:protein kinase A signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008862">
        <property type="entry name" value="Tcp11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12832:SF11">
        <property type="entry name" value="LD23868P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12832">
        <property type="entry name" value="TESTIS-SPECIFIC PROTEIN PBS13 T-COMPLEX 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05794">
        <property type="entry name" value="Tcp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="140"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="309"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="375"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1005"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="142"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="310"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="375"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1005"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="3">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1120" mass="126946" checksum="D50E4673FF2983D9" modified="2017-01-18" version="1">MEHTMDQERQTSTASAQADTSNNNGAGISVGAGDMGVSSSNTGGRDNSNDTEENGMVAVTSGPGNIKPKDXNHLGSNINHTRQYMSNNSSFSRKPLNGLXDLVLKKPLSNDSLHMNVISSSLQSPSSFLEDEHXNGRLQENGLEEQEENSYETENDTTKFGEFPNNDNQMDEERDNKTNIKKNLTNNVEVDMDMDMDLDTEMDADKDTTMKEAYPDSDVQKPHHQLFKQFSTNKGKFEDDGTVTSSEVPQLKTIQKYRSLSEPFLLNATHSVILGSNDVNRPENNNSNIDSHLSSSIQQIISTQLNKNKNNQHHNHNHKHSHLHNNHTPHSILNSHGNDCLVRVKKHLNSTPHLYQVSKKSAQLNLQHLQQQRQKQQFEQLQQSNQIKQRHLDGMEQQQQEQQQQQQQQQQQQQQQQQQQNMQTQLQMQWAQRGAKLTVPQAMHNPPLMPFVNQNKNPANLVAPFQKAQVMIPTIHDSLDKRIRYLLYLQTTFENAPLPPINLECLKEIDLNEIIKNPQLRHDIIFDPLLQFRPNMDGERGLRKKLKADKYWNDVLLEISVIYNNNDKMIEHNDSIGSLTSAPLTQEEYVNKLFRKEKSLLLPLFQSLKDILMSIIPKKDLENVHLIMDIDLILQEFLKKSINLTNFSNWLTTIFKNQCAPMRDSWVDTINENFVTADTNKDIGLLVEALKSTFQLLEAMKLDIANHQIRMLRPALISNSVEFERQYFASLLKSKKIGKSQSVNWIYLSGVSSNSLGSERDADSTSPLAVKQKVTIPEFFHIYVKNVVRLLSCKKMVKEYPITLSFDHTRLSLLRADIRQXVCLLIVRFLFKQLIGNEKDMSSEERIFILKNYSNKNLRDDILSIIIDSHGNCRWTKNTMAMAIHLVKKMHDLRLAFKNQKATGFAPSVETENISTSEKLDATLQSXTASIDKYKIEFAKNWLSKQTQPTSEVYGVLEKRVLDNLVQIITKKSKCSNDGLINQDFMHLVESGLIDNNENLNDSSSALKSKKLGTSSTLAGKPSSAVRANPNGSEHVYGKNEESMXLGPESSAANTTLPATSAPGANAGGALGRANTRVEKNAFDLEEFNALSTNLYIVISLHWSVFGTHYIDFMKDEIAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0T9</accession>
    <name>A0A1E5R0T9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sporulation protein RMD1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4488</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RMD1/sif2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0T9"/>
    <dbReference type="FunCoup" id="A0A1E5R0T9">
        <property type="interactions" value="107"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R0T9"/>
    <dbReference type="OrthoDB" id="18302at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003734">
        <property type="entry name" value="DUF155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051624">
        <property type="entry name" value="RMD1/Sad1-interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255:SF15">
        <property type="entry name" value="SPORULATION PROTEIN RMD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02582">
        <property type="entry name" value="DUF155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="413"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="DUF155" evidence="4">
        <location>
            <begin position="217"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="45"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ80509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="441" mass="49981" checksum="EBD58AF8D469EDB0" modified="2017-01-18" version="1">MSLDGDHISEHDNLLPKQTGSGSEATTTGETNVPVNTAAKTFEHSGLAAPNTGSASSASSASKNKTSGIQRAGPQRMSRVTHKLKLLPEADNINDETNSTLKLENGHNGKEVYSQVNQIKDKPARRDAEKLGKKQRHLLPRVTAYCTCGSYKMKELIRWIKDCRKAHHTHPKLFDECLYTPFIYNDWRGDKRFEHDDIIRLDDEGGEINVSSKQPDLFIYEYGVIVMWGFTEREEKAFLSDLDKFEMEKLAPEDVQVEQLNYYITQSYQPRIYNDFITLKDGSNYLIKLSISHAIAQSVKISLFEELVDNTIEDTQNIPQQIASMGKISMTKEEIMKSIGELFILRININLHGSVMDSPEIMWAEPQLEPIYQATRGYLEINQRVGLLNQRLEVISDLLQMLKEQLGQSDEEYLEFIVIVLVGVEVLVSVVNIIVDIIADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0X4</accession>
    <name>A0A1E5R0X4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">G protein-coupled receptor GPR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g4465</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0X4">
        <property type="interactions" value="162"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0X4"/>
    <dbReference type="InParanoid" id="A0A1E5R0X4"/>
    <dbReference type="OrthoDB" id="5368598at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023041">
        <property type="entry name" value="Glucose_rcpt_Git3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022596">
        <property type="entry name" value="GPR1/2/3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 157-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112:SF37">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR GPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11710">
        <property type="entry name" value="Git3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11970">
        <property type="entry name" value="GPR_Gpa2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="301"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="494"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose receptor Git3-like N-terminal" evidence="7">
        <location>
            <begin position="98"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="G protein-coupled receptor GPR1/2/3 C-terminal" evidence="8">
        <location>
            <begin position="481"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="572"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="891"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="572"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="601"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="614"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="637"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="911"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="946"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="972"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="9">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="9">
        <location>
            <position position="829"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF11970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1005" mass="116339" checksum="012E16C81DF8216E" modified="2017-01-18" version="1">MAVDVNNSLTSTTRKQMRQIMKQIMPQTADDGGTLLKKYQHTTLSNVTTNITTVGKTQMKLLQKTKLLSKKLPTTTVNIVLQLPGLFSTFTAKQLTILRSIAIASSSLSIILVFISMYFLWNMDKRKVVFRHQFIFYLLFCDFLKAATLLIYPITILIRNEVYGQPGLFNTIGFSTQFATEGADLAIFFFALHFALLVFQPKRKYPNKKTGNLEGGLYRYRYMVYFLTLIIPMVLASLAFIDYNKENIAVLKADSDIVLDNLNYNFEPVSKTGGYKPLSTICSLPPWPIWYSLVLSWGPRYAIMASIIGIYIAIYIHVLKESSKIKNQLVNFIGDSSAKSKKDLTKNKIWNSVYQGNNYLMKILYFCKIPQLGMMLIRLFEFDIAEDDEDNNSDHEIKKYRKMFKLQYLKNKSFSTSKNRKFSKVSLLDKAXNPVNENNALEANINPITMGDGTEVQPRKTQDTIDELKNAFNYEVYNQFKQKRLAIKKQVKSIFLYPISYLFCWIIPFVESFIQMKYEEEHGPVVGISYVSTFMHPFNGFVDSCVFLFREKPWKYYWYVVEREELIDFYSAPSSQQHPRRPTVQNSMFTQGTGTSKTLNDADLSSKSSDSSDTVVNYSNGKSLVSGDNGGRLLENDQSDVENNENNDQSDXERNRPNNGSTSKLKDILTFGSEKAAVPAGLTEYIKWELMNTDYGRQGWYYKGTLKRKSCWRHSSSKLKRICWFFYRFRLLFTNGRLTFQDNCNDSKYWNEYYQLSEEEQNLYDFQSYDLVPWYWRLYHILPLKKGVDLDEISLVLKQKRIKEIGDYNNMTLPGXKLVNFNKSRNFKDKVHHIDSLQLXPLDEQAGSKEDAGHANLGFEDPDAAQNIFRRHYHIERKGKQDWYRQQEELQKKHDEEQLSLGSSQKQKQKQKQEQKQEQXGGKKEQANDKSSASLWSTPYDVAHAGGKENYEMDDRTGNIPQMGLHPAKTWSHNTNNSSTNNTTDSYNEDEDQSSSMDIMDFLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1K7</accession>
    <name>A0A1E5R1K7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosome-recycling factor, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Ribosome-releasing factor, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3961</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Necessary for protein synthesis in mitochondria. Functions as a ribosome recycling factor in mitochondria.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1K7">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1K7"/>
    <dbReference type="InParanoid" id="A0A1E5R1K7"/>
    <dbReference type="OrthoDB" id="407355at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.20">
        <property type="entry name" value="Ribosome-recycling factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002661">
        <property type="entry name" value="Ribosome_recyc_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023584">
        <property type="entry name" value="Ribosome_recyc_fac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036191">
        <property type="entry name" value="RRF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20982:SF3">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME RECYCLING FACTOR PSEUDO 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20982">
        <property type="entry name" value="RIBOSOME RECYCLING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01765">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55194">
        <property type="entry name" value="Ribosome recycling factor, RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosome recycling factor" evidence="7">
        <location>
            <begin position="83"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="35"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="243" mass="27199" checksum="E58DFB5D0416E005" modified="2017-01-18" version="1">MFTSKFFXKNLVVPSSIXKSLIQTRSLHPATVPLYAKKSKKSNGNGSSNEKADSXXTPADVLDPKEFKSKVSADLTKLLEQQKKTLAETAKGRNDVKIFDSLTVGKEKFTNVASTSQKGKNLLIITVFDPKNSKNVVSAILGANLNLNPERIPDNDQQLKVVLPPVTQESRNQTLKLLKKYNDEFKSGPFANVRNKYLKDLKPYGFKNEDAKKCSKEIEKVYKDFGDKLLQQYKAVEKLVQQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1L0</accession>
    <name>A0A1E5R1L0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MIOREX complex component 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3951</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RMD1/sif2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1L0">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R1L0"/>
    <dbReference type="OrthoDB" id="242766at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070131">
        <property type="term" value="P:positive regulation of mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003734">
        <property type="entry name" value="DUF155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051624">
        <property type="entry name" value="RMD1/Sad1-interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255:SF1">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02582">
        <property type="entry name" value="DUF155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF155" evidence="2">
        <location>
            <begin position="98"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="334" mass="38259" checksum="3D806FDE99FEEA8D" modified="2017-01-18" version="1">MFXRSXILKPQLSLRNFASISTLYKAVPFAKPVTGVTIGDRFNVEKLLQKYGKAFTKLPNSDIVYGXVKLGSATFEVSSATADFANXQRPDEVKEIFVIPRYGSVCLFDVQEDNINHLKPLVAELEQFVEGDKNSVETEELSYQFXAXAKDQTATPGFENKVQDDTLLLSTQMNPLKVKSTITRAICRSTKLSVLENELENHISKTKDLIYELRHKGTLXGKTETEIIKYCGELFLIRGKLNLYNELXDNSPDVYWSEPLPDLENLYFQISKKLDVVIRINTLNKKLDFATDEMKALLSVLNEKKGTKLEWIIIWLITIEVVIELYHNYERYFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1P2</accession>
    <name>A0A1E5R1P2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Outer spore wall assembly protein SHE10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4352</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1P2">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1P2"/>
    <dbReference type="InParanoid" id="A0A1E5R1P2"/>
    <dbReference type="OrthoDB" id="3260408at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="544"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="415"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="544"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="587"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="633" mass="73924" checksum="99114FAAC0A19E74" modified="2017-01-18" version="1">MKTIXKVVLIPLIALLSVYSLLSIECSGGFKLLSSVDTYHNENDVLYKGASTKSVLNYLPKEWLEHEFKLEKTVNGHNYIXDIDIDIDTDLLKCRNKLCLKMFYFEKNTKINLIKKFHTINENYIQKFYNHLIYDIDYKQIYFRVTASIIEFSSGLMHADKRNQYVQXFKLKFVNLYHSARQFVYQLTNDDANYNXGNEFTSSSVVSIATDGVQSVSTSSIDQEVATKKFNKLEDFDLLFHIIDGKADKINHKLDRKIKKYEHHKLNILKPIFTEKLQELTELAFNHTKIIGKAIIDVNCIPIDTNSSIDTNTNXTKDTNDTKFFNNDTDEECLEFITRDKMRLLFKMAIEDCEQFTNDIINDELNEYVDEINEELGELRELYVDFFEEWADSLMNELKNTLVYQDLHEHVLNNLNIEKNKKPQLEQELKMIESDNDHQNDNDTQETDLLKFLNTINEGDDETDSNDEEAEDIRWDKYMGLKRKMIKKRKELTEKTINVNDLDQFVKDIKTHLKILSREGTENFMILRARANLQFQAREEKEYNQQMLEKERKEQEEAERKNQQLRKAAEDAETAGQKAKNGKLGEGDDEDDEDDDDGVITEVVTEVVVGHVTETFTQTSSTVIMQTQTELVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1R6</accession>
    <name>A0A1E5R1R6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Antiviral protein SKI8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g4332</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1R6"/>
    <dbReference type="FunCoup" id="A0A1E5R1R6">
        <property type="interactions" value="162"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1R6"/>
    <dbReference type="InParanoid" id="A0A1E5R1R6"/>
    <dbReference type="OrthoDB" id="10251741at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051510">
        <property type="entry name" value="SKI8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44090:SF1">
        <property type="entry name" value="SUPERKILLER COMPLEX PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44090">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="308"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="418" mass="46706" checksum="819869F686C41F7E" modified="2017-01-18" version="1">MSKLFIPTVNVGLAHEADIYCLELSQPFTITAGGDGVLKLWKNKILEGQLPKEHVYTLNVNQVGIHHAHYLHYIEPVDQKEIFLVACVGFNGSLHLYEFDLTEKKFHNLSESVKFSGAKDEAGSQLGIDFNESFWSVKFVPKDLINGIETHKLVLATVEGSLISYNFEYLSGEQSFSKDEVTHSEFSFKNKQKINTLTKINKSDGFPLALDCNNVRKIITMGYSNGEVLILELQTLRTLFNISLSPYPVRNLKVSHDGKLLSIAHDMGSYGRMSLVDTDYGEHLGDYTVATHSATAAGSATTNESLIGFAHNKWVLQNDFNESDEFCISGGLDGKLRVWNTIKRERVSTLNMSNLDIEDETKILTHDEHNNNLDNPGILGCKYIPKNYRGGMGSDSNEGLVVVCLDRSVRWYREAGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1S6</accession>
    <name>A0A1E5R1S6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g4298</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1S6"/>
    <dbReference type="InParanoid" id="A0A1E5R1S6"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025928">
        <property type="entry name" value="Flocculin_t3_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13928">
        <property type="entry name" value="Flocculin_t3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184564" evidence="3">
        <location>
            <begin position="18"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="4">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="733" mass="77229" checksum="3B61816235D9D139" modified="2017-01-18" version="1">MLKELLTACWILATANAYMLPRNVPNYEGPLLLKRNTPYYSNTSIDSTPTASVSSAMFTTLHTTIDNVVYXLSSLVTETSTATIDVIDTDIVTITSCSQNQCKTKQLYKTAFETATSYGXTATVYSTYLLSTTTHTLKGTVTEFTTWCPLTQSTAYTISSSSVAPAVQSTSTFFSETFATSTQLKSAEATKVSLVQLGAQSPTSPLSVLTSTKSTYSTFNTDDCPSCSETSKTATSSSSGLTFSLFSSSSXQTSSLAETSTTPDQQTPATITTTSSIYSVITSTYCPTCSSTTIVLSSSIPVSSSFLSTISTKKQLQFSSVSTSSXLIKPITKSSSSSSSTSKTATSESLKSISMSGQTSYHLSTTTVTKHGAVTVYTTWCPLTSSVSVVSXSATNTPFSSSIFASSQSSTTISSASVSTKVSALTSTDLTLTTSTTTQVPTTSTLLLTVLSSSAKFTTGTESPLITSAKTTVTVHSKDETSTQTSTRQLLPTTVTEHGVVTEYSTWCPLSANKNTETLPSVTLTTXSSLQTKYETXKSSATSFQNLGXLTSKSLELTVISGTSPAVQSSVLTSKLAYIITSIQSTTTSATSQEFVSASQTSFKKVFASSSTLAAEPVIVFSTKLNXETSNTSLQTSTDLLTKSYTQNTENSFTFVTSPVTFGTTPATITNNAQFQTQSFSSVTLSLKSSTVSQSTTKASSAYVVQTQYDNKASKTGLCATSILLSILSIIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1Z7</accession>
    <name>A0A1E5R1Z7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">37S ribosomal protein S12, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g4282</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1Z7"/>
    <dbReference type="FunCoup" id="A0A1E5R1Z7">
        <property type="interactions" value="381"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1Z7"/>
    <dbReference type="InParanoid" id="A0A1E5R1Z7"/>
    <dbReference type="OrthoDB" id="361013at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03368">
        <property type="entry name" value="Ribosomal_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000099">
        <property type="entry name" value="Ribosomal protein S12, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_uS12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005679">
        <property type="entry name" value="Ribosomal_uS12_bac"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00981">
        <property type="entry name" value="rpsL_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01034">
        <property type="entry name" value="RIBOSOMALS12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="190" mass="20835" checksum="DDBD718F116E64BB" modified="2017-01-18" version="1">MFTNVVRRLPGVAVPKQRLTALFPVSSFPQNAFLTSTKTQLGSMPFNNLFALNNVRNFHTSPIVSATLNQIKRGAVCPDKKKRTKSPDLQVCPLRKGVVLRVMILKPKKPNSAQRKACRVRLTNGNVVSAYIPGEGHNAQEHAVVYVRGGRVPDLPGVKYHVIRGAGDLAGVVNRVTSRSKYGVKKPTKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R226</accession>
    <name>A0A1E5R226_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Superoxide dismutase</fullName>
            <ecNumber evidence="7">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g4140</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 7">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the iron/manganese superoxide dismutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R226"/>
    <dbReference type="FunCoup" id="A0A1E5R226">
        <property type="interactions" value="611"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R226"/>
    <dbReference type="InParanoid" id="A0A1E5R226"/>
    <dbReference type="OrthoDB" id="239262at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.990:FF:000001">
        <property type="entry name" value="Superoxide dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.55.40.20:FF:000004">
        <property type="entry name" value="Superoxide dismutase [Fe]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.990">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="entry name" value="Iron/manganese superoxide dismutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050265">
        <property type="entry name" value="Fe/Mn_Superoxide_Dismutase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001189">
        <property type="entry name" value="Mn/Fe_SOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019833">
        <property type="entry name" value="Mn/Fe_SOD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019831">
        <property type="entry name" value="Mn/Fe_SOD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11404">
        <property type="entry name" value="SUPEROXIDE DISMUTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11404:SF6">
        <property type="entry name" value="SUPEROXIDE DISMUTASE [MN], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00081">
        <property type="entry name" value="Sod_Fe_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000349">
        <property type="entry name" value="SODismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01703">
        <property type="entry name" value="MNSODISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00088">
        <property type="entry name" value="SOD_MN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="2 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Manganese/iron superoxide dismutase N-terminal" evidence="8">
        <location>
            <begin position="18"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="9">
        <location>
            <begin position="117"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000349-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="220" mass="25347" checksum="528C8DEB69F67F5A" modified="2017-01-18" version="1">MFRSTAVKNLITKRTKISLPELKYDFNALEPFISGKINELHYTKHHQTYVNGFNTATEQFSELKQKLDTDPVTISRKLNQLQQNIKFHSGGYINHCYFWDNLSPVEAHGGQLPSKDSKLGKKINEQYGSAENLIKLINKSLAGVQGSGWSFLCYNTVNDALEIRQTYNQDTITDAGVKVLIAIDAWEHAYYLQYQNRKVEYFDAIWNVINWKEAGKRFEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R265</accession>
    <name>A0A1E5R265_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">N-terminal acetyltransferase A complex catalytic subunit ARD1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4064</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. ARD1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R265"/>
    <dbReference type="FunCoup" id="A0A1E5R265">
        <property type="interactions" value="789"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R265"/>
    <dbReference type="InParanoid" id="A0A1E5R265"/>
    <dbReference type="OrthoDB" id="25586at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990189">
        <property type="term" value="F:protein N-terminal-serine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990190">
        <property type="term" value="F:protein-N-terminal-glutamate acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045047">
        <property type="entry name" value="Ard1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091:SF4">
        <property type="entry name" value="N-TERMINAL AMINO-ACID N(ALPHA)-ACETYLTRANSFERASE NATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="4">
        <location>
            <begin position="50"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ80972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="246" mass="27827" checksum="2741CE643E3BFD0C" modified="2017-01-18" version="1">MPITIRRATINDMLAMQNANLTNLPENYTLKYYMYHLLSWPEASFVATTTDVDAANEVIDGVQKDHASESATTTSQSSENVFQDDIHGNECTIKLDAQYVNKGEKVIGYVLAKMNDDPDAADQTLNGHITSLSVMRTYRRMGVAEKLMRQALFALAEVFQAEYVSLHVRQSNQAALHLYRDTLEFEVLEIERGYYLDGEDAYSMKKVLDVVKMLPSKFQKTDVEDDCESDLLQEITERAMSSGKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2C8</accession>
    <name>A0A1E5R2C8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Proteasome-interacting protein CIC1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3915</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2C8">
        <property type="interactions" value="622"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2C8"/>
    <dbReference type="InParanoid" id="A0A1E5R2C8"/>
    <dbReference type="OrthoDB" id="10251727at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="367" mass="41556" checksum="08C230E0ED65DFD0" modified="2017-01-18" version="1">MSGVVATERINRAYDALSKYLKTKQEDQEEIGSKKMLIADXDEELLSKLQLIFVNNKSFTGSNNKLQFKPLVLPLKHSFYAKMQENGNEMAQGFKILLILNDKHGINIDSDDESSVISKLKQQDGISVDKVIVGKDLKTTFKAFEKRRQFLNDFSLILCDDSIITTMPKLMGGKFYNKLATTPIPIRVSQSATTLYNSVKKFYNDKLVCKIPRGTTLNVSLGDFHWFTKEEAVANIQSVADSIISKYNIRSVFVKTNFSPALPLYFNQESIDVLFKELEQSRKVAAEDEDQDQDDKDLEDGTESVEIGGVEIGLNVFNKALMEIANPDELDVLFKKQITKGKKRRSDEEEVKETPKKTTKPTKKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2D1</accession>
    <name>A0A1E5R2D1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Signal peptidase complex subunit SPC1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3864</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPCS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2D1">
        <property type="interactions" value="150"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2D1"/>
    <dbReference type="InParanoid" id="A0A1E5R2D1"/>
    <dbReference type="OrthoDB" id="263893at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009542">
        <property type="entry name" value="Spc1/SPCS1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06645">
        <property type="entry name" value="SPC12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ81040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="120" mass="13449" checksum="D5F2A30AA0A44A21" modified="2017-01-18" version="1">MSEYLKVVTDKLVFPIDFPSQEYVEVITNKILLIGLFSSCVFGMYTSSVINAFIFYGTTVVITLLLVVPPYHSYNEHKLTWATPXKEKANHSFVSSSGKNKGAKHITGFIKTDVEIEIAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2G0</accession>
    <name>A0A1E5R2G0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Survival factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g4130</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SVF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2G0"/>
    <dbReference type="FunCoup" id="A0A1E5R2G0">
        <property type="interactions" value="123"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R2G0"/>
    <dbReference type="OrthoDB" id="2590239at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051385">
        <property type="entry name" value="Ceramide-binding_SVF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033394">
        <property type="entry name" value="Svf1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013931">
        <property type="entry name" value="Svf1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47107:SF1">
        <property type="entry name" value="CERAMIDE-BINDING PROTEIN SVF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47107">
        <property type="entry name" value="SVF1-LIKE PROTEIN YDR222W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08622">
        <property type="entry name" value="Svf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17187">
        <property type="entry name" value="Svf1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159245">
        <property type="entry name" value="AttH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Svf1-like N-terminal" evidence="4">
        <location>
            <begin position="56"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Svf1-like C-terminal" evidence="5">
        <location>
            <begin position="242"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ81068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="419" mass="46945" checksum="85CFADE77ACB6883" modified="2017-01-18" version="1">MLKWIQGGISSVTGIAEPEYGPECIHSCTTRVSNKQSFNEAVADDFKWQSPENTHVETSTFYFTDLKKGYNGFAQIIHSNIGGIHTTAQFTFKLYKYKNYDASKAGDFTLSEDELAEASKDFETIWTSTKLSNFRIEGKNFFADNLSIEWISDTEVHFKSAVNPASVIDLVFVQEAPSVKVGVDPHTYYGDNLEAPWGSMSHVFRPRNKVTGSIKTTTNSGAEIVLDKEYTKGMFVMALQGMKPHHAAKAWKFINFQNQHYSVVLMEFTTPKSYANTVVSIAIVAKDDKIVSVPVMNDCRMLELTTDDAGWGVPKSINFQLNGFDASLNDSEVETKGIAGLETFEISGTLTELTERVDVMNEMPQFVKNIVSGVAGTKPYIYQFYSQDLLLKLPNEEKPQTGPCWFETTYIVDEEVPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2I5</accession>
    <name>A0A1E5R2I5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Serine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="3">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Seryl-tRNA synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Seryl-tRNA(Ser) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g4219</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the attachment of serine to tRNA(Ser) in a two-step reaction: serine is first activated by ATP to form Ser-AMP and then transferred to the acceptor end of tRNA(Ser).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2I5"/>
    <dbReference type="FunCoup" id="A0A1E5R2I5">
        <property type="interactions" value="832"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2I5"/>
    <dbReference type="InParanoid" id="A0A1E5R2I5"/>
    <dbReference type="OrthoDB" id="10264585at2759"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00770">
        <property type="entry name" value="SerRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.40:FF:000003">
        <property type="entry name" value="Serine--tRNA ligase cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000026">
        <property type="entry name" value="Seryl-tRNA synthetase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="entry name" value="Serine-tRNA synthetase, tRNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033729">
        <property type="entry name" value="SerRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="15">
        <location>
            <begin position="198"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="280"/>
            <end position="282"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="296"/>
            <end position="299"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="367"/>
            <end position="370"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="403"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for serine binding" evidence="13">
        <location>
            <position position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ81109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="460" mass="52880" checksum="20D388D6B75D2EC6" modified="2017-01-18" version="1">MLDINLFIEEKGGNPELIRQSQRARFAKVELVDEIIADYKEWVKTKFQLDEINKSINKVQKEIGLKFKNKEDASELLKQKDTLNEDKKQLTEKENKQDFDLRKKVGTVGNIVHPSVPVSNDEEADNALVRTWKPETYEKIEKIAAATNSPASLSHHEILLRLDGYDPERGVKISGHRGYFLRSCGVFLNQALINYGLQFLAKKNYIPIQAPVMMNKDVMAKTAQLSAFDEELYKVLDGDDEKYLVATSEQPISAYHSNEWFEKPAEQLPIRYAGYSSCFRREAGSHGKDAWGIFRVHAFEKIEQFCITEPENSWEEFDKMIANSEEFYQSLGLPYRVVGIVSGELNDAAAKKYDLEAWFPYQQEYKELVSCSNCTDYQSRNLEIRCGIKKKGETEKKYVHCLNSTLSATERALCCILENYQTEEGLKVPEVLRRYIPGEPEFIPYVKELPKNSTSNKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2J6</accession>
    <name>A0A1E5R2J6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoserine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">O-phosphoserine phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3856</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HAD-like hydrolase superfamily. SerB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.3" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2J6"/>
    <dbReference type="FunCoup" id="A0A1E5R2J6">
        <property type="interactions" value="544"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2J6"/>
    <dbReference type="InParanoid" id="A0A1E5R2J6"/>
    <dbReference type="OrthoDB" id="27226at2759"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00198"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036424">
        <property type="term" value="F:L-phosphoserine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050582">
        <property type="entry name" value="HAD-like_SerB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004469">
        <property type="entry name" value="PSP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01488">
        <property type="entry name" value="HAD-SF-IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00338">
        <property type="entry name" value="serB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344:SF2">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01137">
        <property type="entry name" value="C1.6.1:_Phosphoserine_Phosphat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01136">
        <property type="entry name" value="C1.6:_Phosphoserine_Phosphatas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00029">
        <property type="entry name" value="phosphoserine_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR604469-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ81122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="305" mass="33696" checksum="FCCDB84544EEB5CA" modified="2017-01-18" version="1">MALFLTVIANGDCFEQKSQLVDAFLQRNGLADKPKVVLSANKAFRVDITTNSKDISTQHDLDVVAEDAGDFVAVVNSYDHFKPQLCVFDMDSTLIYQEVIEMIAHYANVEPQVKEITDRAMNNEIDFQESLKLRVSLLKGLPFNELYGEICEKIKITNGVQELTRFLHNINCKTAVLSGGFEPFAQFIQKTLKLDYMKANNLEIDSQTHLLTGRVLGQIVDGQCKAETLVSLANAQETPVPIEKTMMIGDGGNDLPAMAVAGYGVAWNAKPRVQQAAPSKLNSSSLLDVCYILGYNDAEIKEILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R3C8</accession>
    <name>A0A1E5R3C8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Uroporphyrinogen decarboxylase</fullName>
            <ecNumber evidence="4 14">4.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AWRI3579_g4253</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the sequential decarboxylation of four acetate groups of uroporphyrinogen-III (octacarboxyporphyrin) to yield coproporphyrinogen-III (tetracarboxyporphyrin) with the formation of intermediate hepta-, hexa- and penta-carboxylate porphyrinogens in the heme biosynthesis pathway. Acts on a number of porphyrinogens, but only coproporphyrinogen III can ultimately be converted to heme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>uroporphyrinogen I + 4 H(+) = coproporphyrinogen I + 4 CO2</text>
            <dbReference type="Rhea" id="RHEA:31239"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:62626"/>
            <dbReference type="ChEBI" id="CHEBI:62631"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 14">
            <text>uroporphyrinogen III + 4 H(+) = coproporphyrinogen III + 4 CO2</text>
            <dbReference type="Rhea" id="RHEA:19865"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57309"/>
            <dbReference type="EC" id="4.1.1.37"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the uroporphyrinogen decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.37" evidence="4 14"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R3C8">
        <property type="interactions" value="912"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R3C8"/>
    <dbReference type="InParanoid" id="A0A1E5R3C8"/>
    <dbReference type="OrthoDB" id="339900at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00321"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004853">
        <property type="term" value="F:uroporphyrinogen decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00717">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.210:FF:000004">
        <property type="entry name" value="Uroporphyrinogen decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00218">
        <property type="entry name" value="URO_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006361">
        <property type="entry name" value="Uroporphyrinogen_deCO2ase_HemE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000257">
        <property type="entry name" value="Uroporphyrinogen_deCOase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01464">
        <property type="entry name" value="hemE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21091">
        <property type="entry name" value="METHYLTETRAHYDROFOLATE:HOMOCYSTEINE METHYLTRANSFERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21091:SF169">
        <property type="entry name" value="UROPORPHYRINOGEN DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01208">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="UROD/MetE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00906">
        <property type="entry name" value="UROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00907">
        <property type="entry name" value="UROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 14" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="8" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="9 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 14" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uroporphyrinogen decarboxylase (URO-D)" evidence="17">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Uroporphyrinogen decarboxylase (URO-D)" evidence="18">
        <location>
            <begin position="193"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS00907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OEJ81073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="407" mass="46175" checksum="1B8D6F50758F250B" modified="2017-01-18" version="1">MYFKNTLPLYNSRSIKEEAKKLKSRNTSXNDTRNTRMSGNNFEDPTAKFPKLKHDLLLRAARGEKVERPPCWIMRQAGRYLPEYHEVKNGRDFFECCRDAEIASEITIQPVTRFKGLIDAAIIFSDILVIPQAMGMRVEMLEGKGPHFPEPLRTEEDAAKVLDYHVDVLKELDWAFKAISLTRTKLNGDVPLFGFCGGPYTLLTYMVEGGGSKIFRFVKEWINARPELSHKILQKITDVAIEFLAQQVVAGAQMLQVFESWGGELGSVDFDEFSLPYLKEISAKLPLRLKELGVKDKIPVTVFSKGSWYALNKLCDAGYDCVGLDWSWDPKEAVKINNNRVTLQGNLDPGVIYGSDEVISKKVEQMIKGFGGGKQHYIVNFGHGTHPFMNPEKIKFFLEECHRIGSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R3K5</accession>
    <name>A0A1E5R3K5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R3K5"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184586" evidence="1">
        <location>
            <begin position="22"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="405" mass="47676" checksum="03D8C5DD682403EB" modified="2017-01-18" version="1">MMYWINVLFLMFFLCFGKSNGIDHDTQSKNGIVAVQDSSAIYQHLSTLLDDYHHAESNDNGDHKHLVNDYHIVYIYSRVQQEQVIVLQSILNGLLQGCIELYQDDMDENSXNIGEKIETETHEQVYFYGIDVQDPKNVLFLKNSGIMQTNTFHIFQYGQLISTFGLDNGMMLDKKQNGKDTKSYFALFLRQLTKLDHKRELISKIHCGKVKKYDNANVFVYNDVSKIGKLNEIMSHSTKPSHHKAILFSNQWIDPRISYMFDLIWYTVFDVVFELIDQSFITNHLRSNKPLDFINLNGNTMDYYYYDLLNEHHIIDFPTILFVFHNDQTGNDTFKLVNFKKIYNKILLQNGVKILSLKPQELHLEFLVRDLKWFFQECVLSWQDDRVVEDIKSLPNRQCSFYFEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4P7</accession>
    <name>A0A1E5R4P7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cell wall integrity and stress response component 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3785</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4P7"/>
    <dbReference type="FunCoup" id="A0A1E5R4P7">
        <property type="interactions" value="136"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4P7"/>
    <dbReference type="InParanoid" id="A0A1E5R4P7"/>
    <dbReference type="OrthoDB" id="2019572at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051694">
        <property type="entry name" value="Immunoregulatory_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002889">
        <property type="entry name" value="WSC_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15549:SF30">
        <property type="entry name" value="MID2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15549">
        <property type="entry name" value="PAIRED IMMUNOGLOBULIN-LIKE TYPE 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01822">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00321">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51212">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184607" evidence="7">
        <location>
            <begin position="23"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="334"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="8">
        <location>
            <begin position="26"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="121"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="286"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="365"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="405"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="288"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="365"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="405"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="442"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="456"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="477"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="509"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="9">
        <location>
            <position position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ81889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="542" mass="56004" checksum="D4F46364C8F08D23" modified="2017-01-18" version="1">MKNHIPLAIGLLLVQLVHLTLAAQDVFQYKGCYSSSDLLSSVTSQGSYKYQSPSYCEGECEGKAFVALLNGGQCFCGDDASALSSLDASGTCDVGCYGYGVITCGGQSSFDVYVNENADTSSSSSSSGSSSSSTSSSSSSSSSSSTTSSATSTSSESSSEIPTTSSTSSTSSSETSSTTSEQSSTSSTTSSTTSSTSSTSSTSSSSSSSSSSTSSDSSTSSSDFTTSTLTPTSTTSSSSSSSSSTTSTTQNHSTKIVTTKIVTTMQQSQSTGETNLIQTIIVTQTDTASSSESTSDADSSASSSSSGSSSSSGGSASQGKNSSGNSTKSISTGAIVGIVVGVVGGVLVIGIIVFLVWFRHHKKNKEPDMEEKKQYQPYSFGLPEDPDTSQAGMLAAAAAVTASSPSSISNRSIIRNKSNNNSNRSFRSDNDSGNDDFNFVDAEQHSYNDSQRDYYSETSSPHSNQDPENETYPDFDQSGNHSNIFLTDNGEDDHEQSRTSHLGPHPDSDFDNDDQSNDSDDQSDNEMGAKPSGGFRVINPDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4T3</accession>
    <name>A0A1E5R4T3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Replication factor C subunit 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3780</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4T3"/>
    <dbReference type="FunCoup" id="A0A1E5R4T3">
        <property type="interactions" value="811"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4T3"/>
    <dbReference type="InParanoid" id="A0A1E5R4T3"/>
    <dbReference type="OrthoDB" id="4199794at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031391">
        <property type="term" value="C:Elg1 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031389">
        <property type="term" value="C:Rad17 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000004">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000129">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001679">
        <property type="entry name" value="PRK00440.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF9">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="43"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ81927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="341" mass="38424" checksum="8EBA0E8D91E16690" modified="2017-01-18" version="1">MSVVKKGNENLPWIEKYRPENLDEVYGQSNVVTTVRRFMQEGRLPHMLFYGPPGTGKTSTVIALAKEIYGPKYYKNMILELNASDDRGIDVVRNQIKDFASTRQIFSKQQQFKLIILDEADAMTNAAQNALRRVIEKYTKNTRFCILANYAHKLTPALLSRCTRFRFQPLPQSAIATRLETVLIKEHLSLTDRAKTAILKISNGDMRKALNVLQACSSIASFHEDQSNGDQVTSSKEITEDMIYDCVGAPKPAHLEYCLNSILNDDRITSFYTIEKLKRESGLALLDLINGIVEILDDHELSESTRSSILIKLSEIEYAMSKGGNDKIQTSAMIGVIKSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R504</accession>
    <name>A0A1E5R504_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA mismatch repair protein MSH2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">DNA mismatch repair protein Msh2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g3799</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the post-replicative DNA mismatch repair system (MMR).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R504"/>
    <dbReference type="FunCoup" id="A0A1E5R504">
        <property type="interactions" value="1138"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R504"/>
    <dbReference type="InParanoid" id="A0A1E5R504"/>
    <dbReference type="OrthoDB" id="295033at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032301">
        <property type="term" value="C:MutSalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051053">
        <property type="term" value="P:negative regulation of DNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.110:FF:000002">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000925">
        <property type="entry name" value="DNA mismatch repair protein MSH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.10:FF:000009">
        <property type="entry name" value="Mismatch repair ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011184">
        <property type="entry name" value="DNA_mismatch_repair_Msh2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF35">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005813">
        <property type="entry name" value="MSH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="DNA repair protein MutS, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="12">
        <location>
            <begin position="764"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="13">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ81971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="963" mass="109245" checksum="FA2B7DD69F8B565D" modified="2017-01-18" version="1">MTSIRPELKFQDVAEERGFYKRYQNLPKALSNTIRIADREDFYSVVGEDAIFVAENVYHTTSVLKSTTALHQDEPLKYVTMSKQVCYNFLKMCLFDYGYKVEIYDKTWKLIKNASPGNIEQVDDLLTISINDSVVLCSIKFVNNDGQGNCTLGCAFVDSSTYKIGMLEIIDNEVYSNLESLIIQLGVKECLVPDLRDNVNSKRELQKISRVIDRCGCVVTYTKPGDFQPKDVEHDVIKLIGDELSMALPKFSKNSLGACNALLDYLNVLRDTSNYGKYQLVEHSLSQFMKLDASALNALNIFPQSGSQIIGPTFSHNVPGDSAQGSKINSLFQLLNKCKTNSGIRLLNEWLKQPLLDIDELTKRHDLVEYLIEQLELRQILRNDFLPKVPDMRRITRMLMKKKEDLEDVLKIYTFATTIPKIISNIEIFLNEDDMTSASDSKIRQLVESHWLNPLKTQIQPLDTFQELVEQTVDLDSYNEHNEFMIRMDVDETLSSTKTLLDELKGKIREIHYDAADDLNFDPEKKLKLEVHHIHGYCMRLTRNDAKAIRNNRKYMELSTVKAGIFFTTKELKQLAQEVGNIQKDYDRLQADLVREIVKVTLTYVPVLETLSMTLSHMDVLCSFAHVASYAPIPYVRPKMFAKNSRDGEKRKLILKESRHPVLEAQEDISFISNDVCMKRGERDFIIITGPNMGGKSTYIRQVGVITLMAQIGCFVPCDEAEISIMDSILCRVGAGDSQFKGVSTFMMEMLETSSILKNATGNSLVIVDELGRGTSTYDGFGLAWSISEYIASKIGCFTLFATHFHELTNLSTKLSNVVNMQVLAHIDESEKDISAEKEEPNDEITLLYKVADGISDQSFGINVAEVCKFPSKIVKMAKRKASELEEMKEVGQDVKKRCDANDISVGTLKLKSILQTWVKQAETQNLLSQREEGVEKIASLLKDIYEESAKNKFIEEIISDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R510</accession>
    <name>A0A1E5R510_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3524</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R510"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="unsure residue" description="D or N" evidence="1">
        <location>
            <position position="1675"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ81982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1683" mass="193513" checksum="372AFEF62527172C" modified="2017-01-18" version="1">MVDGYTHPVEFLNDNTFSSHDIITESLNYLGDEYDAEHAKDPNVPYDRDFGGPHDFLRTSEFECKPIPTTNQEILLGEPLKIFSLNVFAGKSTMTMDTDKVNDDGLSNYDVSQKPFLDSATIKENFDENSHVRQPVSEIAHDDSQAEMDVDKFEEQPFLSNSNSMPETLESLNESDVEYLLQRKSKTGETLFTNAEAEKSSQKTSKHLKCTVVVTKHYLYVYEKREYKKFGKELVDCCKISSYDESMQQSDILVATFFDGSAALIGLLQNEDFFWNVEILTTVQLSRDEIAKNGPFGYGLRLWNVVSHPHDPRICVFYCMQSSQIKFYNIHSKKFALVGKGNMWCKGHEFHSIXFFPSTYNSTNGENNXNGKFDGVGFSKVLDRNNNSNIFEGTPTTYVLHVVTTFEKSNYSLFVFYYNEWSREDWCVSTKHLVQHRLVEFYKSREAVVPAKNHCSVPLLKQEIVTLNLEKNGFCYYNLLELSSANSDNVNCFAKNVIALNADDDNNENELAXNIKLSFILPCXLSVLKYWKNLYAKELETTESCFIVALSTDHLLMAFVQSNKKLLFGLITKVKGVNNIELCLPEFDETEQMTCGAQSRYLKILVSSKFGLVHSGEVNELIHLEQNKNQGELNALSNCFFSNWQDMLFVYCKNYDNIFDLKQIFVTVPQCEKLLSIPSVAGSTNPSGCELWSIDNKYGATQFLFQRNALTFFPVFETNKFLTALKFFCLPLNEFHDAIVQAHFAPYFDKFTKHTKETNIDKYALFVFALAGSKTTFQLWDLENQEFHCIDHHFENTESLLKGSTSACNVVGKRFLIRAIETTFDDAFASLPLEVYCFGNDGTLSPVHLLGFDMFSPEDAKNMVVEFTAQSYNHVAVLLSVNGVQLCFYTCYRGTGNSHFESADFKTWTFKSVNIENTSFKKINSMQFISDRLLLGSDETTWEYILDDSTDNIKSSKQIDISCLWEQGLIRFNSHLVLNDTGFDFRDMAQSTDWSSLTKLPKVSIEIKNAYTPVERNNHHMQSNGFQTVSYNSHTIHYLDLQSYELLDVDLNMPWNSKHHLIMCCDAIGTNHIAVLFDDKFCILGKNLHSEIKFIPKGNDFINSGDFFASFDGPPFSKDVKGDIWNLTTESVKNVNRILLKKMIGNGVPIFGLSHGATNNYKCDGDTLNKSIFEYCDKVNRVIFFDFKSYLGDSPAVYGMKPHCNVMNKLCKIDDNGIFASLKNFRKDKKLKQRDNNKFDRKLFSKSFRVPSILKVYPSTNSERFNVLVTYNALGSQNNVLKIIKLVPSSKKICLQQVFEYKFCDNDPFGQDVTWIETPLTRTASNTDYYLVWLYNEHENAIKALKFQWNDLNTKITKTSSSLQMVSNCSWVLPPVQKLKQMCSLPGDRLLLLFADNTLGVLVHKLQLKQDVEDLNENFFKNYMHSTHYSTEKEKQKHIHTLGFIDPITERNFNFEYNDIEIANTDFIPFRLKRQQDDFNKLNFEKLSENDSTNVEPLFYPLSKENYKIMELYSQERGVFNISNIDYIVPLPEYSYMKNQNMFLAFDVLALDQSECXINISMMCFKSTDKKTKADYFDEXFEFKKNNHFYTSQVLASKKCTNPXLSSSFNNIFQHQTDFKPILDVKLVPLKENSLNLNNDSTNSIIQHEYYFVHVLLANGQLETLELKEVYPFXDDKPTSTTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5A5</accession>
    <name>A0A1E5R5A5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative sulfate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3722</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5A5"/>
    <dbReference type="InParanoid" id="A0A1E5R5A5"/>
    <dbReference type="OrthoDB" id="427213at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="302"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="388"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="417"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="444"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="485"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="7">
        <location>
            <begin position="562"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="607"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="51"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="8">
        <location>
            <position position="26"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="8">
        <location>
            <position position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ82081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="747" mass="83545" checksum="3B21AF12FB330DF8" modified="2017-01-18" version="1">MPKPVMKRTPSALKSYYSVAREEQQEEQQQQQQSFAGQNDTEFSEQDGPSAENNDYTPQSTSSTTVHMHGLNRYVSMLPLVAWLPQYNTAKFASDLVSGITLASFQIPLSISLSTLAKLPPQSGLFSLIIPPIXYLIFGTVPSLVIGPQAVASMLVGHHIDLVSAKNAIVFQDTSTSTTRDRIQVASTITFTCGAMLLGIGLGKYGFIDNALSKSFQRGFISSLAFMIFISSLMTELDMNEAYKRAGSAQSNGTSWNKLKFIWEHTELIETTSMCITIATVGFLVMTRFFKNKWSNAHPKLIFFPDILFVVAVSTVLSFAYKWKVSLVGELLEKSAIEIRWPLTDFQKFRDSFDMSILVTVIGIFETATAYKTITEQSLLDVSSNKELFAVGLSNVLCSLFGSIPAFGGYGRTKLNILTGVATPLSGIFVSLCSFLVMNYCLKWFYYLPQCSLSSVITLIALTLLEELPKDLLFYWRVGGYREIVVVFTIVVFTLVWSPQMGISMGLAITLVALLQQTTKSNITVLGKDPNTNQFKPIITTFHTSASAPPMHRSASNSSVFTVAERMEFEKIMIVEVPALLIFANTTDFKRKLIKLEKYGPSSSSKFPTTKKFASQGQKGKFHPNSETQRLIRIRNEPLRYLIIDFENLHTLDVSAIQSLKVIVQSYSDRGIFVFFTVAPKVATLXEKLKQSGILVAQKRLVQEWLMNKTDFSQDFLNLVHTDLFESIESAVECIDYMEYIERNHDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5B3</accession>
    <name>A0A1E5R5B3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADPH-dependent aldehyde reductase ARI1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3603</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R5B3"/>
    <dbReference type="SMR" id="A0A1E5R5B3"/>
    <dbReference type="FunCoup" id="A0A1E5R5B3">
        <property type="interactions" value="268"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5B3"/>
    <dbReference type="InParanoid" id="A0A1E5R5B3"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000191">
        <property type="entry name" value="Methylglyoxal reductase (NADPH-dependent)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="6"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ82094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="345" mass="38229" checksum="370FCE1E4ACA7E04" modified="2017-01-18" version="1">MTKQTVFVSGATGFIAQHIIKQLLDSNLYKVVGSVRNSVKADKVMRDFGNNTDLTLVIVEDLSNLDAFDSVFEKHGATFDYVLHTACPILSADNDFQKNLITPAINGSKNIFEATVKYAPNVKKFVQTSSYAAMRNPDANHNPNITVNEKSWGSLTLQEGLQNGWSAYFYAKTSAEREVWALHKSLNARFLLTVINPTFVFGPQCFDSSVSKTLNSSCEIINAIVHSKPSDPPQQDIKGNFIDVRDLARAQVQCITDDKLNGQRLLLSAGKYCWQLIANIINTEFPELRGKIAKGEKGGDKQVLNSFAKIDNHVTKELLGFEFTDLETSVTDTVQQILKVEATAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5G1</accession>
    <name>A0A1E5R5G1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Mitochondrial respiratory chain complexes assembly protein AFG3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AWRI3579_g3652</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="17">Component of the 850 kDa m-AAA protease complex, a heterohexamer composed of YTA12/RCA1 and YTA10/AFG3. Associates with the prohibitin complex, composed of PHB1 and PHB2, inhibiting the activity of the m-AAA protease complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5G1">
        <property type="interactions" value="495"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5G1"/>
    <dbReference type="InParanoid" id="A0A1E5R5G1"/>
    <dbReference type="OrthoDB" id="1413014at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005745">
        <property type="term" value="C:m-AAA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097002">
        <property type="term" value="C:mitochondrial inner boundary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140567">
        <property type="term" value="F:membrane protein dislocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065003">
        <property type="term" value="P:protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000019">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000003">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1690.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050928">
        <property type="entry name" value="ATP-dep_Zn_Metalloprotease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011546">
        <property type="entry name" value="Pept_M41_FtsH_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655:SF2">
        <property type="entry name" value="AFG3 LIKE MATRIX AAA PEPTIDASE SUBUNIT 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06480">
        <property type="entry name" value="FtsH_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="19">
        <location>
            <begin position="382"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="78"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="92"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00065348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OEJ82108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="818" mass="90669" checksum="75FE6B17D1E5F4AA" modified="2017-01-18" version="1">MSVLISRKICGGTHAMKSGASGLRLSVMNGLRKKSINTKFFNNEDTLMKLSQYRAFSXQFMFGNSYFNSVRNKNSPVQTRSFHKSARLYNQKNDDQSEKNNENKDGDKQKDKENNKKQKKNDEANYKNMMDYFKSKEFQKTLIYTFTFSFLYTYFTAVLLKNSEENGVHEPLTFQDFKVKYLEKGLVKRIFVVNKQYINAELVTSSLGSDVQGTEYADGSALSSSSSSSQSTLGQTNYENHGQMVSFTIGSVDFFEDEMDKVQDQLQIPPHERIPVIFVEQISMLQYLFPFVPTILLLGGLYYISRRFQAGGAGAGGFGSSGGKNGGIFQVGKSKAKLFNEETDIKVKFQDVAGCDEAKQEIMEFVEFLKTPEKFTKLGAQIPRGAILSGPPGTGKTLLAKATAGEAGVPFLSVSGSEFVEMFVGVGASRVRDLFAKAREMTPCIIFIDEIDAIGRSRGGKQGGGMGSGGANDERESTLNQLLVEMDGFETNEQIIVLAGTNRADILDKALMRPGRFDRHVEVDNPDVEGRKAIYKVHLKSLKIQPSLLENLDLLSGKLSALTPGFSGADIANCCNEAALIAARHNDNFVEMKHFEQAIERVIAGLEKKTRVLSPVEKKIVAYHEAGHAICGWFLKNADPLLKVSIVPRGQGALGYAQYLPGDMNLMNNLQIKDRMVMALGGRVSEELHFNSVTSGAHDDFKKVTGMATSMIKSLGMCSRIGYINYDESKSASNGGGVLINKPFSEATSQKLDEEIKKLVDECHEACKQLLQSHMPHIEKVAQLLLKKEVLTRQDMIDLLGPRPFEENNPAFTKYLDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7E3</accession>
    <name>A0A1E5R7E3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative oxidoreductase ENV9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3431</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7E3"/>
    <dbReference type="FunCoup" id="A0A1E5R7E3">
        <property type="interactions" value="256"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7E3"/>
    <dbReference type="InParanoid" id="A0A1E5R7E3"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF282">
        <property type="entry name" value="WW DOMAIN-CONTAINING OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ82825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="354" mass="40073" checksum="2F072E6459C48367" modified="2017-01-18" version="1">MEHKYADLATLPYTDPEKEHRVAIVLNGDKKCGYHISLNLYNHGYTVYIGCRNVANYTKAYEQIKLQVNKTNSGTKTKRKNNAKQETAEKKNFGRLEYLPMDMKDLKTVMRAVLRFQKAEERLDLLIDSGGGYIGVAHQTTKDGFDLQLQTNYVSQALCALLLLPQINHAKGKIIFMSSVCHFLQYQTCDAFEKQTYDYSPSFIFSWFRYGLAKTLCIQFLKILAIKNPSITCYSVNPGIVINTNIFSSLTTLPVLGIFFWLFFQMVTLLFGVSEQKGAASVVQLALGRERSLPNTKEHNTKSSVDLVSGKYFNKYGIAINPSPVANNLDNSASTWILTVRLLRDRNIQIPSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7K3</accession>
    <name>A0A1E5R7K3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3434</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7K3"/>
    <dbReference type="InParanoid" id="A0A1E5R7K3"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184660" evidence="1">
        <location>
            <begin position="18"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="102" mass="10764" checksum="3A370749F02D4B21" modified="2017-01-18" version="1">MKSSTIALLSTAAVASAAYYGNTTETILEPTTTMTSRILITVTLGTDRFTLTQTPLAPTYSSHWNNTSTASSAYLNSTVDYSQMKLSNASSPANATNGFALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7L2</accession>
    <name>A0A1E5R7L2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Very-long-chain 3-oxoacyl-CoA reductase</fullName>
            <ecNumber evidence="12">1.1.1.330</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">3-ketoacyl-CoA reductase</fullName>
            <shortName evidence="12">3-ketoreductase</shortName>
            <shortName evidence="12">KAR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Microsomal beta-keto-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g3264</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the microsomal membrane bound fatty acid elongation system, which produces the 26-carbon very long-chain fatty acids (VLCFA) from palmitate. Catalyzes the reduction of the 3-ketoacyl-CoA intermediate that is formed in each cycle of fatty acid elongation. VLCFAs serve as precursors for ceramide and sphingolipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a very-long-chain (3R)-3-hydroxyacyl-CoA + NADP(+) = a very-long-chain 3-oxoacyl-CoA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48680"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85440"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="EC" id="1.1.1.330"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Endoplasmic reticulum membrane</location>
            <topology evidence="12">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12 13">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.330" evidence="12"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7L2"/>
    <dbReference type="FunCoup" id="A0A1E5R7L2">
        <property type="interactions" value="703"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7L2"/>
    <dbReference type="InParanoid" id="A0A1E5R7L2"/>
    <dbReference type="OrthoDB" id="5545019at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045703">
        <property type="term" value="F:ketoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141040">
        <property type="term" value="F:very-long-chain 3-oxoacyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030497">
        <property type="term" value="P:fatty acid elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05356">
        <property type="entry name" value="17beta-HSD1_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03107">
        <property type="entry name" value="3_ketoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027533">
        <property type="entry name" value="3_ketoreductase_fungal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43086:SF2">
        <property type="entry name" value="HYDROXYSTEROID DEHYDROGENASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43086">
        <property type="entry name" value="VERY-LONG-CHAIN 3-OXOOACYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000126">
        <property type="entry name" value="11-beta-HSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 12" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 12" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0521">NADP</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ82884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="337" mass="37362" checksum="68865D0774DA1D84" modified="2017-01-18" version="1">MGLLSVLYKGLFILGAAKVFQLFFRLIQCCLQLFVLPATNFAKYGGTKKSNSQKKYWACVTGASDGIGKEYAYGLAARGFNVLLVSRTESKLVELQKNLVQKYGSQGVEVDYLSMDCAQNKDADYAALKDKIANKKITVLVNNVGLSHDIPVSFLDTDEKEMNDIITINNVSTLRTTKIVLPNMMESVSQNGDVKGLILTMGSFGALFPSPLLATYSGSKAFLQNWSSALSGELKEHGIDCELVLSYLVTSKMSKIRRSTVLIPTPKQFVRSVLDGCGYRGGAQERMSTMTPYWSHGLYHFVVEETYGCYSSLVNMINLKFHKSIRSRALKKRAKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7T2</accession>
    <name>A0A1E5R7T2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Glycerophosphodiester phosphodiesterase GDE1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3346</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7T2">
        <property type="interactions" value="180"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7T2"/>
    <dbReference type="InParanoid" id="A0A1E5R7T2"/>
    <dbReference type="OrthoDB" id="197419at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047389">
        <property type="term" value="F:glycerophosphocholine phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057506">
        <property type="entry name" value="C2_GPCPD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051578">
        <property type="entry name" value="GDPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22958:SF1">
        <property type="entry name" value="GLYCEROPHOSPHOCHOLINE PHOSPHODIESTERASE GPCPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22958">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25329">
        <property type="entry name" value="C2_GDE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SPX" evidence="6">
        <location>
            <begin position="1"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="616"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="7">
        <location>
            <begin position="1054"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="915"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1155"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1177"/>
            <end position="1188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ82956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1424" mass="158747" checksum="79C330C15D3A79ED" modified="2017-01-18" version="1">MKFGKTYGNHQIPEWSDFYLDYKQLKHTIRQISTQQDAILALDKESACKQEPSLNNSTVSLTSNSIIESSPETVLATLQNPKVKNLLSQFLIEFEKNIEKVDEFYNKQFSEYDRRLRRLLNDPIIIELQNSNADAAGNGFNGKSSSQXNFSEGKTQGFSTFSTHSSFMNSNDAANGSSLNATGMNDNHINHYHHGLPEKEEMDEYIAILVELRSNFRKLKWFGDLNKRACIKILKKLDKKCGTDQQQRFLQEGVFSLDFSNPAEIDIDVNKVNDLLQLLSYDSKSLAKSLGKQANGNGNDESEDSPPSVITQLIQKDDEKGLINELIYTYRSVVLIPTRLLVSLLNKAALLQSKKCIDEILEIIPSLEDANDINKRNFFHHHVIALGKNNFKSSQKKVEKSIDGLPVAISNSVDSANSLMETNDSASFYTSTSTLGKFEGINDLLNMESNTKFLYPAMPPERQLIGAYGSDGINSSDSPNALKYILEKLPVHLRASLLKKDTYKRTPLHYAAQYGLVEITKIIITILKXWDVWNPSTPIDDRAVWGDSDNLTPLHLAVMGTHPVTVETLVSFVSXDVKTSSPKLLFLATRLNSPTLIESLLSINGFDVNYKETGKTGDTALHVATRLNLLDAAKCLIKNGASTEIKDKNYGWTPVFIAASDGLEEMCELLVREAHCNIHIYDDSGWTPMEHAALRGHLKLSKLLAIDDPNIMNPIMTMDEPERSKSPFPNQNMNKCFYKASTPSLVSLKDANTNGSSSNLSMETIIQDSKKSRIPTKKSIIEDSTSAQQQQQQQTMALDVSSVTSKAVKSFGHEFLKHTESFISITLGSNDTSNPIPAITLQKVPFAKASSTELDTALSLMITCQDSLNGEPSILDLPLDETINAVNFKVPFKTNMHHVVYFDLVPTYGVNPATHKKGQSSETDTFGFGSSGNLKHSSLRRSEDSQISISRSNALSQYGSLPLKRVPSQKPQRLVLGRAIALLDSANTRVGKNKRSLQDTVTVPIISSESMEILGTINFEFLLVNPFTPSNPSFFEKTTRWLNSDTYWKQLVSTRVIGHRGLGKNIDSKKTLQLGENTVESFIAAASLGASYVEFDVQLTKDDVPVVYHDFLVAESGVDIPMHELTLEQFLDLNNINDDKKDAAKDLLDKNFNHKDEKITQESQXSGRETPFRRRRSVDDTDLKNSREKDAKASLESFWERRMRYTKTYKEKNYKGNFRGSSIASSFVTLKEVFRKLPKNVGFNVECKYPMTDEATKEEMSQIAIEMNHWIDTVLQTVYAHCGKRDIIFSSFNPDVCIMLSLKQPHFPVLYLTEAGSTVMTDSRALSLQSAVRFARTWNLLGIVSAAKPIVEAPRLAHIVKSSGLVLFTYGVLNNDPAIARREILAGVDAVIVDSVLAVRKGLTKEALSTSSNSSEESINDILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7T7</accession>
    <name>A0A1E5R7T7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Peroxisomal membrane protein PEX25</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3348</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7T7">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7T7"/>
    <dbReference type="InParanoid" id="A0A1E5R7T7"/>
    <dbReference type="OrthoDB" id="411017at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652:SF50">
        <property type="entry name" value="PEROXIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ82957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="374" mass="42937" checksum="8D2A9EC694AD8CD7" modified="2017-01-18" version="1">MSMDMGNDTQSVFFSSNIFTNEKKNXCATEENIIIADNIRSGENNKNTIASGDNHTXAKKTIAIVPSTRSTVRNIDILLQLFRTLAGKDKIAKITKYVLDLVKLILVKKQGTNRLNTTPTNKTHTMFASLLQNYNWNKFQISNIPLLDILNTLSVNIGIYRQXLRFGWSVFNLEKIVXRLRELVTVNAKSSDTISKPXIKPLNEDDLVLFVEVYYNVVDELLLLRKLKVWDNPILYKKLAKHEALSWYYDIILSFKANLIKYHKIKTHILEMNIKLELKERCLQNLELNSSLKNQMLQDIGSTVEIEDEIAELGKDLKLVKLDLFRLFWDFLADTTDVFDMKTPPGTYAVLSLCSGITGFVKLWISEKRKSNXL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7X6</accession>
    <name>A0A1E5R7X6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dihydrofolate reductase</fullName>
            <ecNumber evidence="2">1.5.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3403</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the dihydrofolate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.3" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7X6">
        <property type="interactions" value="438"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7X6"/>
    <dbReference type="InParanoid" id="A0A1E5R7X6"/>
    <dbReference type="OrthoDB" id="414698at2759"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00158"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004146">
        <property type="term" value="F:dihydrofolate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046452">
        <property type="term" value="P:dihydrofolate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046655">
        <property type="term" value="P:folic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00209">
        <property type="entry name" value="DHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="entry name" value="Dihydrofolate Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012259">
        <property type="entry name" value="DHFR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017925">
        <property type="entry name" value="DHFR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001796">
        <property type="entry name" value="DHFR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48069">
        <property type="entry name" value="DIHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48069:SF3">
        <property type="entry name" value="DIHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00186">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00070">
        <property type="entry name" value="DHFR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="Dihydrofolate reductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00075">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51330">
        <property type="entry name" value="DHFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHFR" evidence="8">
        <location>
            <begin position="3"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ82971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="230" mass="26211" checksum="0CD48918F3FBEDE3" modified="2017-01-18" version="1">MKPVVSIVAALVPEYGIGYNGTLPWKLRKEMQYFKKITTTTLDPSKKNAVIMGRKTWESIPPKFRPLPERLNVVISSKYPDTWNLLENTGSTTSCSSAADTVIKYNNLQQSIANLQKMNDVERIYIIGGAQVYNATFDLATHLLITEIQMADKPNEHGDNESLAINQNIPPPMDTFLDAISIQRKFVKKDTQTWNSFTLNTFNTQDTHTEGNYKFQFTLYEPKLKRSLEX</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA64</accession>
    <name>A0A1E5RA64_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RA64"/>
    <dbReference type="OrthoDB" id="3971756at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="439"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="110"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="307"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="439"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="466"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="496" mass="54482" checksum="A36A13B17639EE20" modified="2017-01-18" version="1">MTHSIQRIPFLSQIEDMEAYVSQYKDMKNNNNTNMIMNLNNAANNYNYNNSTNNGNSSYGXNQNHINNPSSSVMHTVQSYNNIHHDQQNNKNNNNNNNNRHHHNHHNYHNNHSTDNNNNNNNSNSNHYQNHQNGYKGXNANHKNNNGNGNMNGGNGASMQSYGSQLQVQRSGGVLNPTSYSSLYLNNDMASAPNYLNVPAHFTNGIYNGSALDLESVTGITTPARPAXSTPLYHNSNGYDQLLHQRSNLVSPGLSTPDGLLSSQTHVTRSQPPYINDFYKQKNETGLGQSTSLYNLQNSMQSNPFDSNKSMMSSSNNNSNSNSNTNNNNNNNNNNNVSIXDNGNHMINDNTQGNSSLNTGKMNGLYSMNSTSPFASSTASFNDGGLYTGNQTASPFSYTQHXGASSTGGVAMNGHSGSSGQHLPLQQLEQLQLQNLQNMNQPQQQPQQPSGQHQFLSQQPNLQPFNSAGSSSGQLSNSSMLSPYNKIWSDNMSVWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA73</accession>
    <name>A0A1E5RA73_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Epsin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2948</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the epsin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA73">
        <property type="interactions" value="112"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA73"/>
    <dbReference type="InParanoid" id="A0A1E5RA73"/>
    <dbReference type="OrthoDB" id="4033880at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16991">
        <property type="entry name" value="ENTH_Ent1_Ent2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000006">
        <property type="entry name" value="Clathrin interactor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF110">
        <property type="entry name" value="EPSIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02809">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="8">
        <location>
            <begin position="11"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="143"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="309"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="445"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="146"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="176"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="322"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="454"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="9">
        <location>
            <position position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ83806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="469" mass="53555" checksum="DC6437F450E6ABA5" modified="2017-01-18" version="1">MSKQLFRSAKNMVNGYSSAQVLVRDATANDTEGPSVDQLDELADRSYNGDEFFPIMDMLDKRINGKGKYWKHVAKSLTVLEYLVRNGSENCVLWCQENLYIIKTLREFMYTDESGFDQGQLIRVKAKELTSLLRDEERLKSERALAKNRRSDRRRRKPKRPNEEXDLQRAMAESMRTAREEEERRKGLNGEGSDEDLQVALQLSKEEEELKRLQDLQKQQQMMQQQPQGSMYYDVFGNPISAEEYMQYQQQQQYLAQQQAEQQALYQQQMAQQQAQAQLQQQQQYLAQQQYLAQQQYLEDQKKQNVLSQYGNNPFAKNPDEPSASVSQQAQPVSIAPPAVEVQSQQQKPMVKTRTGDTAMTEKYGQLNTLLASGTGIDTFGNTGDLRIPAQHTKTGTFINSQGTGYYQTNNEEVAKKHNPFLINQYTGLPSSKIAPVQTGYGFGNQQQAQQTQPSASVNQNTSFSLIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAA8</accession>
    <name>A0A1E5RAA8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 19</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MED19</name>
        <name evidence="11" type="ORF">AWRI3579_g3027</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAA8">
        <property type="interactions" value="92"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAA8"/>
    <dbReference type="InParanoid" id="A0A1E5RAA8"/>
    <dbReference type="OrthoDB" id="2160599at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013942">
        <property type="entry name" value="Mediator_Med19_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28270">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28270:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08633">
        <property type="entry name" value="Rox3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="144"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="218"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ83827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="240" mass="27083" checksum="B7A3B909578605EB" modified="2017-01-18" version="1">MSTEETIPAYYYYIDPLATPYPEPQPNPMENLIDMYGLNDISQQVARYNADGSRGVKLRKSYKNQVSDIAGHWTTENIPTKAKYGDLASKIFHMNHGSNSERESRSNMDADTQMYDSMDWDSYAHVIQSFDNANIMNYISSSPGNGAMNNGSNGAPLQQQQQQQQQHFSVEDLAFDLDGTXSTGGKRKRKFANLQQQQQQQQQEQQQQQQQQGVQQQGVATSASSSGMNTPLDAKRRRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAE2</accession>
    <name>A0A1E5RAE2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3100</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAE2">
        <property type="interactions" value="622"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAE2"/>
    <dbReference type="InParanoid" id="A0A1E5RAE2"/>
    <dbReference type="OrthoDB" id="10009520at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20356">
        <property type="entry name" value="Rcat_RBR_HHARI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="152"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="156"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="12">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ83841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="536" mass="61764" checksum="423F12F08BDC5BCE" modified="2017-01-18" version="1">MDYLDDSEYEEVEIEFESDEDEADIYLSXPPTTTTDTDNIALEKSGVQVISDGINSMELIAKKFKIPIDSKLHTAATYSVFSETELIDYKLKELSSSYFNQVDEVELKQLLMSYDWDIAEILKEKXXFGDQKVLARAGLLPXNKGRIYTKXKEYTCMICCETRNETEIXALELCGHEFCTDCYKQYLLATTSNSSGPPKCMGNCETLIPYKDLQSLMGPSYLDKLFVFSIKSYINKNTEHYKHCPSNNCQEVIAVYDSLEPYLKHHVAPIVSCNDNHIFCFGCRKEDHSPCDCAISQEWVKKTSKESKALNWVLANTKECPKCSVNIEKNGGCNHMTCQNCEYQFCWVCDSNWDSHKGSNFTCVVYRNNVAPENGKTGKKNLKAKENIKYSFQRFRHYYRLFSDCEQSSTLDLKLYSEIEKRITKLQENVGISWIEGQFLDECMKEMIEARKVLKWSFVLIFYSDPSQNFTKLFEDHQMHLMSSIDKLAELFAVTDPEKVFSKKSEYLNLSAFIKTRKRATTEAGRDLIKLKVAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAF5</accession>
    <name>A0A1E5RAF5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Derlin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2622</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May be involved in the degradation of misfolded endoplasmic reticulum (ER) luminal proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the derlin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAF5"/>
    <dbReference type="FunCoup" id="A0A1E5RAF5">
        <property type="interactions" value="488"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAF5"/>
    <dbReference type="InParanoid" id="A0A1E5RAF5"/>
    <dbReference type="OrthoDB" id="19102at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007599">
        <property type="entry name" value="DER1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11009">
        <property type="entry name" value="DER1-LIKE PROTEIN, DERLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04511">
        <property type="entry name" value="DER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="270"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="287"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="305"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ83889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="315" mass="35705" checksum="D1AA38AC021ED423" modified="2017-01-18" version="1">MSSMQPVTKTLLTLTIGITLLPVLNVVPIYSLIFVNIKPYVFTQWWGVVTSFFLITNTSNPLPVLMRIYALYSHSNELETAHFMRPQQFIYYLLFNSIMVLLFTFYFPWNLSWCFEPCLQMFLSYTWSNVNYNKQIRFYGIIPLPAKYSCLMEIILNLVFSSGDFVASSGILGLASAYVYHCLATHSFGPIYGFFNYKLLGRTEKPYIIVKSKTLNGKFKEMQMPNPDFKQGSNNNYGYGGGAPLYPQWFNKMSNLVWNSLVSTKEKPLVNTSKKAGHGRRLGSLPAQSSTSARSNAAASASARFKGEGRRLGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB34</accession>
    <name>A0A1E5RB34_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ER membrane protein complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3069</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the EMC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB34">
        <property type="interactions" value="192"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB34"/>
    <dbReference type="InParanoid" id="A0A1E5RB34"/>
    <dbReference type="OrthoDB" id="124397at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039856">
        <property type="entry name" value="EMC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12760">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="177"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ84102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="328" mass="38633" checksum="B1DA99542FC57652" modified="2017-01-18" version="1">MTSLLNISSESIKEKLLSIQATGYYKQLEPKEIKDVYTQCKTFMKFYEHTLTPIEQYAIMELVFHLALYMSEDLECEKIYKSFFDKFGNNSPYMHYFRSLLQQINNFKKIDTGAAEDSMVVEKSIESLIEEILEVETDQIDYLIMCKRFLSMKRQSISKEIWIKIATGLIEKFPMDTEMWYQLGCDYFELSNFDQAIYCLEEVILLTPHNFVAWSKLGEVYYYKSLSIDDNNSKIKEKTKEKNFLLNESLNRFLKSVEYSENFMKSWCYITVICNXLLSQKNSDTSNNSNNNKQQLFELGKVQLEKYKKQGFMNESDKKACKAILSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB46</accession>
    <name>A0A1E5RB46_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">G2/mitotic-specific cyclin-4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2936</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB46">
        <property type="interactions" value="1813"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB46"/>
    <dbReference type="InParanoid" id="A0A1E5RB46"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044772">
        <property type="term" value="P:mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20512">
        <property type="entry name" value="CYCLIN_CLBs_yeast_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000001">
        <property type="entry name" value="G2/mitotic-specific cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046965">
        <property type="entry name" value="Cyclin_A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048258">
        <property type="entry name" value="Cyclins_cyclin-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001771">
        <property type="entry name" value="Cyclin_A_B_D_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00292">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 4" id="KW-0195">Cyclin</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="328"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="7">
        <location>
            <begin position="421"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="425"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="144"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="144"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ84120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="562" mass="64927" checksum="DBFA119F03EEFE0D" modified="2017-01-18" version="1">MNSSGSNRSVFAGTTGPTSLVSEVDENYKPPQPKATYKQHIRYLPTNTTYGKXGESVSSGPNLGNRTALSDVTSKISNTRHSHGQYVKSSAPLKVKKNYISSRHNNVGPLDQPKNWVEEIRSDPEDSTHDKDVSIMEIDETAHNEKHXTQQHDMQKQDSGNNDVNEETENNISDSSVVIVSEPYQKLQKNIDNVSEEENKSHQLDIKKIRKKDXVGLEEKQXERIEEPYTNRIMSLQGPEPSNPVQNVRFGPVDHLHRASYLKTAFEKYHRLTPDPDDEDTYDPTMVVEYCNEIFEHTSRLEKKLCADPNYIDYQPDLTWKHRHTLLNWLVQXHEKFNLLQETLYLAVNIMDRFLSHTEIALDKFHLVGSTCLFIASKYEEINCIVLKDLLKILDDEYTESQVIRAERYILCALEFELGWPGPMSYLRRISKADEYDFDIRTLAKYLLETTIMEPSFVASPPSWIAAGAYYLSLVIFRDSYCITHDGLVYDDIWTSKHTYYAGYTEQQITPAAAVIAQNCRYAETKHEAIFKKYSQKRHRKCSQMVAKWIEYLEAQERELKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB68</accession>
    <name>A0A1E5RB68_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Inhibitor of growth protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g2550</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ING family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB68"/>
    <dbReference type="InParanoid" id="A0A1E5RB68"/>
    <dbReference type="OrthoDB" id="3971228at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15505">
        <property type="entry name" value="PHD_ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028651">
        <property type="entry name" value="ING_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333:SF103">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="14">
        <location>
            <begin position="297"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="231"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="231"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="10">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="10">
        <location>
            <position position="310"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="10">
        <location>
            <position position="314"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="10">
        <location>
            <position position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ83813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="365" mass="42127" checksum="759E5AEA2B81FBB3" modified="2017-01-18" version="1">MARSNGKRSFGKRKNKNKGRQIWQNAKNTKISNVDATLLNEVNKLDGSDSCISAPMEEKLYSIVKASSSNDIRNQFIPQLDHFVCKLVRQSVWLHCSEDGDIDNTLRTDDDDXLNNHDSKRLKHVRRLVQLVKQNRQDIIDRMEEVRKYSTILREYNRYYGDETTKEAIESNDSVHIENRVIPLKTNNKAVPVNVARAAPVKQTKEQTKEQLLKKNVTRLQQRREIQKVEEAQKAKEAQKAKEAQKAKEAQKAKEAKKTKEAQKAKEAQKAKEAQKAKEAQKVKEAQEEQNSDDDNTPYCICRKPSSGKMIACDNPKCPIEWYHYKCLNLHSTPKGEWFCDQCKSQRKKKHGNKTTRKHKVSSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBC6</accession>
    <name>A0A1E5RBC6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 11">Cystathionine beta-synthase</fullName>
            <ecNumber evidence="4 11">4.2.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g2563</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>L-homocysteine + L-serine = L,L-cystathionine + H2O</text>
            <dbReference type="Rhea" id="RHEA:10112"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:58161"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="4.2.1.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.22" evidence="4 11"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBC6"/>
    <dbReference type="FunCoup" id="A0A1E5RBC6">
        <property type="interactions" value="939"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBC6"/>
    <dbReference type="InParanoid" id="A0A1E5RBC6"/>
    <dbReference type="OrthoDB" id="728at2759"/>
    <dbReference type="UniPathway" id="UPA00136">
        <property type="reaction ID" value="UER00201"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004122">
        <property type="term" value="F:cystathionine beta-synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019343">
        <property type="term" value="P:cysteine biosynthetic process via cystathionine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000052">
        <property type="entry name" value="Cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000003">
        <property type="entry name" value="Cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000118">
        <property type="entry name" value="Related to CYS4-cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005857">
        <property type="entry name" value="Cysta_beta_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01137">
        <property type="entry name" value="cysta_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 10 11" id="KW-0129">CBS domain</keyword>
    <keyword evidence="11" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="12">
        <location>
            <begin position="380"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="13">
        <location>
            <position position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ83873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="513" mass="56396" checksum="51760FC38C5A7B77" modified="2017-01-18" version="1">MPSAQFHNFSTDVLDLVGDTPMLKLNKLPAALNIKPQVYCKLELYNPGGSIKDRIAKTMIEDAEKSGLIHPSRTTLIEPTSGNTGIGLALIGAIKGYRVIITLPEKMSNEKVSVLKALGAEIIRTPTEAAWDAPESHIGVALKLEKEIPGAIILDQYNNPNNPLAHINGTGREIHKQLEALNLIDNLHGVFAGAGTGGTITGISKYLKKNVSDKIKIVGSDPEGSILAVPNTLNETELASYKVEGIGYDFVPKVLERDTVDKWYKTHDKPSFKYARQLIAEEGLLVGGSSGSAFEAMVEYVKEHPELTEKDVLVVIFPDSIRSYLTKFVDDEWLKKNDLWDDEFDEIINGKKRARAAAATNNASSDEKDLYKGATVASLKLKPVVSVVENESFSKVASIMKENGFDQLPVLTEAGKLCGIITLSQLLKVLQNGSKPETIKNVFVDFRKLNNFDEVSSYNDNKSGKKRFNKFSVTTKLSELNTFFEKNSNAIITDDNLKPVHIVTKMDLLGYLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBD3</accession>
    <name>A0A1E5RBD3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SET domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3132</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBD3">
        <property type="interactions" value="124"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBD3"/>
    <dbReference type="InParanoid" id="A0A1E5RBD3"/>
    <dbReference type="OrthoDB" id="20872at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034967">
        <property type="term" value="C:Set3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46462">
        <property type="entry name" value="UPSET, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46462:SF3">
        <property type="entry name" value="UPSET, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="6">
        <location>
            <begin position="365"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="257"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="844"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="12"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ84207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="868" mass="98427" checksum="EB7ECA72236B70A6" modified="2017-01-18" version="1">MFTKTPLEPPRSGQNKAYRSHNSSTSTSRKNSFTSPLSVKSSHLSATTRTPGSKIASPLRARPDYQYHYALSPGPALAILSSHPSSNNLTDYQNTQLALQSPLHYHHQNSKIDKNIIKAMNEERGKIAAAALAASENVPLPLKKPIQQLREEDIQARVKRGQPVTLEQYKVDKDEGIITCVCGSNEDDGFTIQCDSCFRWVHASCYHIEDIDLVPDYFTCRFCEKLENLKSQGEIKEIKAEDSLDFLKSKESEARAVTQEQYELKKKPKKESSNDSANKDSSESNQRQQPKQKDSGKKQNKQKQQEGPPNDGIIQRVKLKQSKDMHSAIYFPLRANEYVDKYVEEFVQSHNDEDWIITPKTFKNLKLEVKPYKEEKQFSGVAKLGCFLKADCVEKNGLIHEVVGEIDYQRKYIMDPRNQYRIWGTAKPKVLFHPHWPIYIDNRLSGNDCRYLRKSCHPNVELVTIKIDSNDTPLGKNMISDPKIKFVLRALRPIEVGEELTINWGWDLQHPIWKLIHTQNLEDIPEAERYFLIHTVESILSFCDCACSNNKECYLRMVKKYYIQLFKRNKTKMNNKYKLNEILYKPKNKEGKQSSILLKLQTSLGEQEKQAQVYYDSYIAKRKLYLEKLAKAKESGHDFDANSLLLNDQISKNELSSEKAVDQNDSALFGKKSTKILGNYKLQLVHDSKTAENFQKAESLKETSEQSSGSQNKKRNLSVFLHSLHDETQVTDINNLPIPIMLKPSVLNSINTILESKKLGSPSMISPNISPVSANSXSFPTVGAGSGSSGHFPNPQTVAQKGISAISAKPVSTHFATMMSMPRANLDIGSLHDSHQSSLVHGVYPTAEPAEKKKKLSFADYRRKSKPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBF8</accession>
    <name>A0A1E5RBF8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Arginine biosynthesis bifunctional protein ArgJ, mitochondrial</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="10">Glutamate N-acetyltransferase</fullName>
                <shortName evidence="10">GAT</shortName>
                <ecNumber evidence="10">2.3.1.35</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="10">Ornithine acetyltransferase</fullName>
                <shortName evidence="10">OATase</shortName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="10">Ornithine transacetylase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="10">Amino-acid acetyltransferase</fullName>
                <ecNumber evidence="10">2.3.1.1</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="10">N-acetylglutamate synthase</fullName>
                <shortName evidence="10">AGS</shortName>
            </alternativeName>
        </domain>
        <component>
            <recommendedName>
                <fullName evidence="10">Arginine biosynthesis bifunctional protein ArgJ alpha chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="10">Arginine biosynthesis bifunctional protein ArgJ beta chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2502</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-glutamate + acetyl-CoA = N-acetyl-L-glutamate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(2)-acetyl-L-ornithine + L-glutamate = N-acetyl-L-glutamate + L-ornithine</text>
            <dbReference type="Rhea" id="RHEA:15349"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.3.1.35"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10">Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterodimer of an alpha and a beta chain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="10">The alpha and beta chains are autoproteolytically processed from a single precursor protein within the mitochondrion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ArgJ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.35" evidence="10"/>
    <dbReference type="EC" id="2.3.1.1" evidence="10"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBF8"/>
    <dbReference type="FunCoup" id="A0A1E5RBF8">
        <property type="interactions" value="307"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBF8"/>
    <dbReference type="InParanoid" id="A0A1E5RBF8"/>
    <dbReference type="OrthoDB" id="2017946at2759"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00106"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004042">
        <property type="term" value="F:L-glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004358">
        <property type="term" value="F:L-glutamate N-acetyltransferase activity, acting on acetyl-L-ornithine as donor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006592">
        <property type="term" value="P:ornithine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02152">
        <property type="entry name" value="OAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.340:FF:000002">
        <property type="entry name" value="Arginine biosynthesis bifunctional protein ArgJ, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2330.10:FF:000001">
        <property type="entry name" value="Arginine biosynthesis bifunctional protein ArgJ, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.70.12:FF:000002">
        <property type="entry name" value="Arginine biosynthesis bifunctional protein ArgJ, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2330.10">
        <property type="entry name" value="arginine biosynthesis bifunctional protein suprefamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.340">
        <property type="entry name" value="ArgJ beta chain, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.70.12">
        <property type="entry name" value="L-amino peptidase D-ALA esterase/amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01106">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002813">
        <property type="entry name" value="Arg_biosynth_ArgJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016117">
        <property type="entry name" value="ArgJ-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042195">
        <property type="entry name" value="ArgJ_beta_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00120">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003802">
        <property type="entry name" value="PRK05388.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23100">
        <property type="entry name" value="ARGININE BIOSYNTHESIS BIFUNCTIONAL PROTEIN ARGJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23100:SF0">
        <property type="entry name" value="ARGININE BIOSYNTHESIS BIFUNCTIONAL PROTEIN ARGJ, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01960">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56266">
        <property type="entry name" value="DmpA/ArgJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 10" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 10" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_5023530258" description="Arginine biosynthesis bifunctional protein ArgJ alpha chain" evidence="10">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023530257" description="Arginine biosynthesis bifunctional protein ArgJ beta chain" evidence="10">
        <location>
            <begin position="218"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="10">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="10">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="10">
        <location>
            <begin position="217"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ84226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="444" mass="47764" checksum="AFEFC4AEE6286FB0" modified="2017-01-18" version="1">MKVTKTLLNSGKKVVDKYALYVPKTGVFPKGYEVGSFASGVKKNGNLDLGVIVNTNRTNFESSASAVFTTNRFKAAPVLKSKQVLENSQYRNINAIVVNSGCANSMTGEVGMKDAEAMCQLVNTTLGTPDSTLVMSTGVIGQKLQMNKISHGITEILQNKSNYGSDFPAWLNLAKSICTTDTFPKLITSSFKLSTTGKEYTLTGIAKGAGMICPNMATLLGFIVTDLPISPKGLQKMLSFAGQRSFNCISVDGDMSTNDTICMIANGAVDTPTIDVESKEFNEICEQVTGFAQKLAQLVVRDGEGATKFVTVKVQNSKTFDDAKIIAETISNSMLVKTALYGEDANWGRILCAIGYSKLDNVTSLVNEKLNVSFVATDSSEPAELPLLVNGVPNLNVDEARASEILALPDLKIVVDLGTGSEECNFWTCDLTHEYVTINGDYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBG3</accession>
    <name>A0A1E5RBG3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SEH-associated protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3052</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat mio family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBG3">
        <property type="interactions" value="809"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBG3"/>
    <dbReference type="InParanoid" id="A0A1E5RBG3"/>
    <dbReference type="OrthoDB" id="341486at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16691">
        <property type="entry name" value="mRING-H2-C3H3C2_Mio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037593">
        <property type="entry name" value="MIOS/Sea4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049092">
        <property type="entry name" value="MIOS_a-sol"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031488">
        <property type="entry name" value="Zn_ribbon_mio"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16453:SF9">
        <property type="entry name" value="GATOR COMPLEX PROTEIN MIOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16453">
        <property type="entry name" value="WD40 DOMAIN-CONTAINING PROTEIN MIO FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21719">
        <property type="entry name" value="MIOS_a-sol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17034">
        <property type="entry name" value="zinc_ribbon_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="MIOS-like alpha-solenoid" evidence="6">
        <location>
            <begin position="514"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="domain" description="GATOR2 complex protein MIO zinc-ribbon like" evidence="5">
        <location>
            <begin position="928"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="468"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="468"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF21719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ84232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1040" mass="118957" checksum="B8DCD70446AF4059" modified="2017-01-18" version="1">MAIIKQALGWANNPEDYVYYLSVNPTKDEVGFYKVINESSDENDDSIIKLDNFKDFQSISALDYSECITGMVGVGEKNGVVKVFNVMEQNHFGNNMFLNQVANGTENDQRSLTSEEEAVQKDEGITAKLLVKPKHQRYVNCLSFSSTRSLVAMGLEKEKHDHSLQIWDVNYQSNANEYVNTAFQFFLNESIISCEFLSDTNLLVSSPKMLREVDLRLPKPVFQLPTSFGNQLKVNPFNSYTFATYSENGTSSIWDRRKLNNKALDYQDYTPSLQFDKAATFYNSTASNSAFPAINEKHSTPSGSSSKPFNHLSFRWSTVRPDEFSTLNNSSTIKRWRLGQVPSESAELTYDSLFVSTVNTVNLPFDRVVTFDYIPRKNNKTSFLCMRQSGTVYRCSVLDSTSKIDFSPSNNLISADYDKLEMNELDVETKLSHSAVRDSLSAALEPLSLEALDKPSGEYQDEMYHSSLETKFREKRDSTNTQDTEESYETERGSEYNMKIDSLALLQNDISMIMRRRANLDYGMEPMSTVDIIDKMQYLQNSAFIRNTWRWLAIAKGSVDDGTMISEKLDIGYEGILGIWNGLDGLAKQNRCRTNEDLTEQELINEMGMIIKMKNKTSLEGNGSNRHVFMKHTKKLVQRKLCMIISGWDFSNEEMEEKYVNLMQRGQYEKAAAWAVFFGDVPKAVHILGQSKRERLKLIATAVAGYSAFQENETNNAWKEQCRKMATELDNPYLRAIFSYISENDWWEILYDTSISLRERLGVALRFLNDKDLSKFLARAARNVISSGELEGLILTGVTPSGVDLLESYVAKTSDVQTAALISIYGSPRYFKDERVENWIGIYRDLLNAWELFTKRSKFDVLRTKLSRNSSGRVTNKTAPRQLYIQCIKCKNNINSVEGESKNMMRKKRKQQYSXAHGDYYYENENETAQQKYSCPHCGTSFPRCAICLLPLGISNLPIMIQGGLQDKENHEDTFFNPTESYVPEENHDLQKEKKRLKFNEWFSLCLTCNHGMHAGHAEEWFERHYVCPVPGCSCQCVYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBG5</accession>
    <name>A0A1E5RBG5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcription initiation factor IIE subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2507</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TFIIE alpha subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBG5">
        <property type="interactions" value="150"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBG5"/>
    <dbReference type="InParanoid" id="A0A1E5RBG5"/>
    <dbReference type="OrthoDB" id="361102at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005673">
        <property type="term" value="C:transcription factor TFIIE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039997">
        <property type="entry name" value="TFE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017919">
        <property type="entry name" value="TFIIE/TFIIEa_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002853">
        <property type="entry name" value="TFIIE_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024550">
        <property type="entry name" value="TFIIEa/SarR/Rpc3_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097:SF7">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02002">
        <property type="entry name" value="TFIIE_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00531">
        <property type="entry name" value="TFIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51344">
        <property type="entry name" value="HTH_TFE_IIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH TFE/IIEalpha-type" evidence="5">
        <location>
            <begin position="14"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="295"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="398"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="296"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="341"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="403"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="439"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="461"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="476"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="500"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="510"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ84221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="523" mass="59217" checksum="E23FB54F5B7DD8D5" modified="2017-01-18" version="1">MNSMNTDKRVEDLVKNLLRFVVRGFYPTQYILMVDCILFHSVLAEEDLAHLLGIKRPDVRALCTKLIEDRLLSSHSQPEFQPGTRGVRRYYYFIKYPEAIDAIKWKIHQLVTVLKEDLQRNSRPQGYLCPVCNTKYSQIDAVALLNYDRTQFICSLCEVPLVEDDSSKKAKEKQAKLSRLMTQVEPIINYLKKIDDSKIEENTFELALTRLIPPQNNSLAYYTVNAKQKRRQDFVKARLREATLSQSAQAVLAAQSATNVVSASVNGVLNNINSRHAGAKSQATLHVNITTASDEQAQKDAQERKXEEKRRQNALPTWHEESTIGKSLGRLDEDDEDYFDSNMNGMNNTGVANSDGNAGSNGNGYYKKNYSDYNKYKEHMQEKEQDKVLSEYYANLAKKNDSDEGGDDEFEDVDNYNYDEDELDEDEYFDDVDDLEGEQAGKEKEKQKEPVAGPSALQGKNEPSPSDVGGSSTSDAETRKQLSEDSTLKETNTDVSVADTETKETKDAAPAEDEEDDFEFEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBP2</accession>
    <name>A0A1E5RBP2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Restriction of telomere capping protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBP2">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBP2"/>
    <dbReference type="InParanoid" id="A0A1E5RBP2"/>
    <dbReference type="OrthoDB" id="289228at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="1">
        <location>
            <begin position="281"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="550" mass="61607" checksum="F60BBB07202BF7BC" modified="2017-01-18" version="1">MGQSVSQGSTSNISSENDAVNEKVSLFYQHPDEILPRLPNLVFEQNMKQFKEASNVKFEELIPILGLDPALDPRVCAIMNNIFSRLSQFAMVNKQTSLVDYHSFKMACLILAFPNLCGKLLSTHNQDYVKLLFMSLVPIDPDHENEKSSTPFSEKSGTVSTTQSIDELALPSVSASIILLFLTFITSISHFCLLKCKISPTYMHDHWVRYQRGPAFNILRTINPNITSQNISKESVTLEQFRFALQDEFPKLLTSLNLLFHAFFELKHTSLPPAVTLQESTLFNLELRSQLATFMSSDFVYANIIKLYSGKDVGFSMRAIQAKVLKWNAPTLMIIKGQLLSDEDKNPRYXKFITEEYPKLRGSHQGGDKEVVTYVVYIDEPWKVTNKNLFANGNTQIIEIQPTQTVHKATGSGSLGNVYFNVMGGGIGVGNSLQPTIKNSDKKYHPGNVSLTIDSGLEFGCFRNVGYGGLFDPGSSYSSGNEQERRFIIQGMEIWGCGGEEVLKEQLEAWQWDENESKRRQKINLKSVGEDRALLEMAGLVGQNQSGGSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RC48</accession>
    <name>A0A1E5RC48_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">60S ribosomal protein L43</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3079</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RC48">
        <property type="interactions" value="1048"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RC48"/>
    <dbReference type="InParanoid" id="A0A1E5RC48"/>
    <dbReference type="OrthoDB" id="10258345at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.30:FF:000002">
        <property type="entry name" value="60S ribosomal protein L37a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00327">
        <property type="entry name" value="Ribosomal_eL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050522">
        <property type="entry name" value="Ribosomal_protein_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00280">
        <property type="entry name" value="eL43_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003058">
        <property type="entry name" value="PRK03976.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ84153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="117" mass="13104" checksum="D9C0EB9D8BE435A6" modified="2017-01-18" version="1">MLLPRSYQLKKKXTHTHTXKYHIXHRAKRTKKVGITGKYGVRYGSSLRRQVKKLEVQQHARYDCSFCGKRTVRRGAVGIWSCASCGKTVAGGAYTVSTAAAATVRSTIRRLRDMAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDN8</accession>
    <name>A0A1E5RDN8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L28</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2044</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDN8"/>
    <dbReference type="FunCoup" id="A0A1E5RDN8">
        <property type="interactions" value="1020"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDN8"/>
    <dbReference type="InParanoid" id="A0A1E5RDN8"/>
    <dbReference type="OrthoDB" id="61900at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.100.10.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L27a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_uL15_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00475">
        <property type="entry name" value="RIBOSOMAL_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="6">
        <location>
            <begin position="39"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="116" mass="12974" checksum="E51BD478B0D3BC71" modified="2017-01-18" version="1">MAGGQHHHRINLDKYHPGYFGKVGMRYFHKQQNHFWKPVLNLDKLWTLVPEQKRDDYLKNASASSAPVIDTLAAGYGKVLGKGRIPNVPVIVKARFVSKLAEEKIRAAGGVVELIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDT9</accession>
    <name>A0A1E5RDT9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Carboxypeptidase S</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1954</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDT9"/>
    <dbReference type="InParanoid" id="A0A1E5RDT9"/>
    <dbReference type="OrthoDB" id="3064516at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05674">
        <property type="entry name" value="M20_yscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017141">
        <property type="entry name" value="Pept_M20_carboxypep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047177">
        <property type="entry name" value="Pept_M20A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE/HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962:SF1">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE_HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037217">
        <property type="entry name" value="Carboxypeptidase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="23"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="9">
        <location>
            <begin position="286"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="543"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR037217-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR037217-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="573" mass="64147" checksum="87BA553FAC6A1E0E" modified="2017-01-18" version="1">MSGYIPIDASDALRRVKAKKKQVTISGLVLAFFLIVFLVLRKTSSNDFEIPECRDCGSYKALQPAFTGQINEILYDEDYKLXVIGKLSRAVQHPTQIFDDTPAPTKNTLLSDDWKNFTSFHTYLEETFPHVFSTLKVEKVNFFGLLLTWEPETATENKPVVLMAHQDVVPVEPNTINKWTHPPFEGFYDSEKDLIYGRGVCDCKQLLISQLEAVEKLIQDGYKPENRALILSMGFDEEASGTYGADQLSKFLENRYGKNGIYAIVDEGGSVAIDNGDYFAGPVGTEKGYLDAWITLNTPGGHSSVPNAHTSIGLISLLNVLLENDPYEQAITPNNPVLEQLQCYAKYXNMNPELKKAISKNDIETIGELINNDLSLKYYFQTSQAIDIIKGGVKSNALPEQVTVLVNNRIGIHSSVQDVVDHILKHLQTVAEEYDLTITLEKDSGKQVSIREEGANGDFVFRMFSSLEPAPISPTGNDTFWEILAGTTVSVYAHDLFNTNGTSNVYVAPSLSTGNTDTRFYWRLTNRIYRFTGALEDIETNEHTVDEKITGKSLISNIAYMYEFIPNIDKYAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE32</accession>
    <name>A0A1E5RE32_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">U2 small nuclear ribonucleoprotein A'</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1885</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the U2 small nuclear ribonucleoprotein A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RE32"/>
    <dbReference type="FunCoup" id="A0A1E5RE32">
        <property type="interactions" value="1294"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE32"/>
    <dbReference type="InParanoid" id="A0A1E5RE32"/>
    <dbReference type="OrthoDB" id="433501at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044640">
        <property type="entry name" value="RU2A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552:SF6">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="235" mass="26548" checksum="7DD6EFEE90827ED1" modified="2017-01-18" version="1">MKITPSTIIDAPLYFTGLKSHKNTEKCIVLRDLELENDNITLTSNLKLISAGTSVIDMTNNSFHEVPDFSDNSDCETLLLSRNLISYVDCAALPKNLQNLQLAHNQIKSLNQFRNWKSSAPTNMTNLSLQGNDICFMADYRLYIIAAFPRLKILDFHKISDFERQKAKQLDLSVFEKTFNYENTAVTSTDKSLNLMKSVVSKLDEATKEELQKQLASATTLEEMDRLEKLLSGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE33</accession>
    <name>A0A1E5RE33_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Imitation switch two complex protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1883</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE33">
        <property type="interactions" value="296"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE33"/>
    <dbReference type="InParanoid" id="A0A1E5RE33"/>
    <dbReference type="OrthoDB" id="332390at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018501">
        <property type="entry name" value="DDT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028942">
        <property type="entry name" value="WHIM1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028941">
        <property type="entry name" value="WHIM2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013136">
        <property type="entry name" value="WSTF_Acf1_Cbp146"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32075">
        <property type="entry name" value="ISWI CHROMATIN-REMODELING COMPLEX SUBUNIT YPL216W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32075:SF6">
        <property type="entry name" value="ISWI CHROMATIN-REMODELING COMPLEX SUBUNIT YPL216W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02791">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10537">
        <property type="entry name" value="WAC_Acf1_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15612">
        <property type="entry name" value="WHIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15613">
        <property type="entry name" value="WSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00571">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50827">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51136">
        <property type="entry name" value="WAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WAC" evidence="8">
        <location>
            <begin position="23"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="DDT" evidence="7">
        <location>
            <begin position="442"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="248"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="355"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="760"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1114"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="730"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="283"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="295"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="311"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="788"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1118"/>
            <end position="1133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1224" mass="141648" checksum="F8645C130B6B20F0" modified="2017-01-18" version="1">MVLYKRSPIILPDPPLLPENLNIKVWLIAETGEWFTEYEDYLSRMDYYNQKHFSCEITGLSQLNFFQAMNSEEVEFENLERKFPLKXREPIAKMLHFNTTTNLAILVELVYNRFKNDYFPGEQVFLKYELASVNATPWIIKEKALFPALVNTQTGETTREAFSKYQIYNFTTNESRIVDEDSICRERSCFTKHLIKSFCKISLFKPFKKQGCPWCIKPEYLNYYNLTMDWPKELLHYKELETKIGYISGAHDDDDDDEDDYDDNNDYDDDDENPHENGIDINDMSSTAKASNNSPREKALIDISDKDEKSNXNAXTNSTKNPVGRPKKHKIEDFFEXKSSNKRGRKSTAEKLAKVNGNGVVSNTKLEDGTPTPGPGSNGSSVNLKREXESQVPEKPVIKITSITEDFGIPFDPAHISPFKNFHKFDANLNEVSITNKKQGTFQEVGKLMIVYQFVNTFNKILKIPLISLDDLITSLKCSNPQELAGETVHLRLALPPQSIDANKEPKNNGQRVSKYDAEIMKQYNKDGLFNVIISQDDPTQTITDTLIDDIDYNGTVILVELFSALIRLFIDETGNWSCIVQESWLDDNPEAQKEISEEMQGFVDKIVDYKKTNWTDRVSKRQFQNGFWLVCLIGIFQDAXHFPKYRXIIINIISKIMITDTKGKNSNAWNRQLWRNFVWKLSLEEKIQCLWVLVELVSCYQMDIKSNIEISMEVVTQIKSERYKTAKDYKATLKKHHELELQMIQLRKKLQDFEVEKNIEKPQQENGTSVDXSNMEPKTTTTTTTKTSEEKVEEKEKVDTDSWNEELQKIQAELEELETSRKNLESDKNYWELQLRANSEQRMKPLGFDRFGNKYWYIELNGIDYTSQDAQDYHQCGKIFVQGPNELDVQFYFNASQKEYMENLXKYKYQEDGSLINFETNAILIDSLGXVQRDFSEIXPLERKVVEEGLDSMLIDDTCWAMIPDQETYASLCTWLNKFGLRESPLKKALEADAEXITXFYKDSLQRNQAAENSNGKEXSRLLKEIQKNQFNSSEEELIKQYVXSFENISIEDKEEAHKRAKIIEVDQKLDDVVQQIMTLEDSASNAREXNDELSLLESAKANLMEEKLQLLEQTKDSPSLPSSKSSDGSVKSNHEFAKLFRSAMEPKIEKLQDLLSELHNSKYSERMEDCSNWENKVALKEYGKFLWKGANNNGREEPPLPTAEERMSVIFSNVPKHDVIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE43</accession>
    <name>A0A1E5RE43_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 6">Peptidyl-tRNA hydrolase</fullName>
            <ecNumber evidence="1 6">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1685</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = an N-acyl-L-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5 7">Belongs to the PTH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1 6"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE43">
        <property type="interactions" value="114"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE43"/>
    <dbReference type="InParanoid" id="A0A1E5RE43"/>
    <dbReference type="OrthoDB" id="1711136at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00462">
        <property type="entry name" value="PTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1470">
        <property type="entry name" value="Peptidyl-tRNA hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001328">
        <property type="entry name" value="Pept_tRNA_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018171">
        <property type="entry name" value="Pept_tRNA_hydro_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036416">
        <property type="entry name" value="Pept_tRNA_hydro_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00447">
        <property type="entry name" value="pth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17224">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17224:SF1">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01195">
        <property type="entry name" value="Pept_tRNA_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53178">
        <property type="entry name" value="Peptidyl-tRNA hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01195">
        <property type="entry name" value="PEPT_TRNA_HYDROL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01196">
        <property type="entry name" value="PEPT_TRNA_HYDROL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0820">tRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ85171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="243" mass="27199" checksum="92D8C8820326689B" modified="2017-01-18" version="1">MFYNSFRHQLHGHFYCXPKRLLTTSTHSKTAPKLVVITGLGNPPDQYANTRHNAGHLVLDCLIPQLVENTNSVSKNKWKNKTNSLSYYISPKTQNESIKAPIQYVFVKVDGCFMNLSGQVLLPFWRSSVIAEWQKLHSMGEVHHIVLHDELEKPLGECQWRKFGTSAKGHNGLRSIDSCNTSAKKGPSGNVAYSKIGIGIDRPANGENIANYVLSRFSRAELELLQKKTIPGVLKLLKQHGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REE1</accession>
    <name>A0A1E5REE1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription factor TFIID subunit 8 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1667</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REE1"/>
    <dbReference type="InParanoid" id="A0A1E5REE1"/>
    <dbReference type="OrthoDB" id="2193813at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019473">
        <property type="entry name" value="TFIID_su8_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10406">
        <property type="entry name" value="TAF8_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor TFIID subunit 8 C-terminal" evidence="3">
        <location>
            <begin position="193"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="133"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="563"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="409"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="138"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="564"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="586"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="604"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="660" mass="75527" checksum="BDF0765B4427A6E1" modified="2017-01-18" version="1">MRDAKQQEPSSTMKQLEKIELKQLPGLVALNLQNDNDVAQLLYKSVAVLLKSMNIHISKLAMEDMHQIVXNNVDNMMMQLHQIIENQRRLQTASLDDLTGFMELFMIENSDLVYELEKSQYIHKVLQENGSLPESLLNNHGHNNNNNNKQRSTFXNSKSNSNEDSEXMIKDLEYVXELTKLLPNENKELISKLPTWLPDLPPAHTYKSTKMFTTKKYDEIVFKKNLVHQQHEISNNINNLENSIYQEMNGKVETEKEKLMGKSTGKSMERFAGNNIKSNISSKKLQSRLRSENKSIFNRVTNLEDYQDIPANLLVESMKTSSMKSTKFDVLDYCINSNKRSSKXKKIEXLKNSNKKNPFIRGMDLQKNNKLMENFIKKYVKRSYDGFYKNTILQSDRIAELKKLWVQQYVEQEKTYKTLKREKRKTLXEKLKLEKELKEQEELLKQQTLAKPIVLNIKPLLSSTITNTPTPPTPIAIPVPNIVSDLSKDSSNQPFLSTSPFVITLKYNKAKYLANGGDQTXLMXPRPLSNGKALQATLEHSEXREVALDADTRGKINELVLNDQASSQQATSNQSLNKQGSETQEAEDKDKEQXTEGQQKEQGNRAXQXEQXEQAEQAEQAEQAEQAEQAEQAEQAEQAEQVEXVEQVEQVEQSGXSGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REG7</accession>
    <name>A0A1E5REG7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleolar protein 58</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2107</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for pre-18S rRNA processing. May bind microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP5/NOP56 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REG7"/>
    <dbReference type="FunCoup" id="A0A1E5REG7">
        <property type="interactions" value="1678"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REG7"/>
    <dbReference type="InParanoid" id="A0A1E5REG7"/>
    <dbReference type="OrthoDB" id="6780543at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.90:FF:000003">
        <property type="entry name" value="Nucleolar protein 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.4070:FF:000001">
        <property type="entry name" value="Probable Nucleolar protein 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045056">
        <property type="entry name" value="Nop56/Nop58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012974">
        <property type="entry name" value="NOP58/56_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894">
        <property type="entry name" value="NUCLEOLAR PROTEIN 5 NUCLEOLAR PROTEIN NOP5 NOP58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894:SF1">
        <property type="entry name" value="NUCLEOLAR PROTEIN 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08156">
        <property type="entry name" value="NOP5NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Nop" evidence="10">
        <location>
            <begin position="283"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="401"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="424"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="444"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="471"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="492"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="523" mass="58190" checksum="1CBB0766D8452867" modified="2017-01-18" version="1">MAYVLTETPAGYALLKASDKKIYKSDTLLKDLNSSEKVLKEFKVAAFSKFQSAANALEEATSVIDGQVSEQLKNLLEETKSDKKSTLIVSETKLANSINKLGLNFKVVSDAVTLDIYRAVKEYLPELLPGLSDSDLGKMSLGLAHSIGRHKLKFSADKVDVMIIQAIALLDDLDKELNTYAMRCKEWYGWHFPELAKIVTDYVAFARIILTMGVRTNAADTDLSAILPEEIEERVKAAAEVSMGTEITSTDLDNIKALADQIVEFAAYREQLANYLSNRMKAIAPNLTQLVGELVGARLIAHSGSLISLAKAPASTIQILGAEKALFRALKTKHDTPKYGLLYHASLVGQATGKNKGKIARVLAAKAAVALRYDALSEDRDDSGEIGLDVRAKVESRLSQMEGRDLKTTKRVSRQQKKVEMTEARAYNADADAATTTEAAPASDSDDEDMEEEKESKKESKKDKKRKRADSDDEEDEEEEKEEEKKEEKKAKKEKKEKKDKKEKKDKKEKKEKKEKKEKKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REI1</accession>
    <name>A0A1E5REI1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Elongation factor 1-alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2433</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REI1"/>
    <dbReference type="FunCoup" id="A0A1E5REI1">
        <property type="interactions" value="1917"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REI1"/>
    <dbReference type="InParanoid" id="A0A1E5REI1"/>
    <dbReference type="OrthoDB" id="3969558at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03693">
        <property type="entry name" value="EF1_alpha_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03705">
        <property type="entry name" value="EF1_alpha_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000003">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000005">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000211">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_A">
        <property type="entry name" value="EF_Tu_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004539">
        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00483">
        <property type="entry name" value="EF-1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008969">
        <property type="entry name" value="PRK12317.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9 11" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="10">
        <location>
            <begin position="5"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="457" mass="49906" checksum="1F09D456CD524C40" modified="2017-01-18" version="1">MGKEKSHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYQVTVIDAPGHRDFIKNMITGTSQADCAILIIAGGVGEFEAGISKDGQTREHALLAYTLGVKQLIVAINKMDSVKWDESRYQEIVKETSNFIKKVGYNPKTVAFVPISGWNGDNMIEPTTNAPWYKGWEKEVKGNVVKGKTLLEAIDSIDPPSRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMVVTFAPAGVTTEVKSVEMHHEQLTEGLPGDNVGFNVKNVSVKEIRRGNVCGDSKNDPPQNSVSFNAQVIILNHPGQISAGYSPVLDCHTAHIACRFDELIQKNDRRSGKKLEDAPKFLKAGDAALVKFVPSKPMCVEAFTDYPPLGRFAVRDMRQTVAVGVIKSVEKGALGAAKVTKSALKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RES6</accession>
    <name>A0A1E5RES6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1836</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RES6"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="101"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="458" mass="51973" checksum="AB58D50B8E808287" modified="2017-01-18" version="1">MPNFPINFSLSSNKENLPALKNTIXNVFTKPVNKDMFSQTKCHESPMQDTDSFSKINKFYQLKEKCKDVFHKWCFHKMCFHKSCFRQNKTKTPVYSQAKGSEASEASEANEASEAXEASEASEASEASEASEASEASDQNAQYGQVSSILYCSKEAEQTEQTGEIAQEDQTISGTLPLHPIISTSPSNNSPVSDESSILSFLEAVETEASTSVEPAAEKIFGFAKTLSITFDAENSEQSQPLRILPPRNAXPLRSALKASSNARTTVPSLDCLDKEKTIKDFYLSVPSKAYQTSMKKVKKSIKLRKMKLKWKSFVHKPNDKWFSLRIPQEENSLDFAPKTFCEWHYLYLQTMILLSQIEERKVTVDLGLPRRRHTKLQNGKIVENSKFITHLSNLYYQKETLATVQPQIVSGEFDPNGYISTFKCPTGMPDYVYQDEKCRLLLVLAKYFEYILEEQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REW7</accession>
    <name>A0A1E5REW7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoinositide phosphatase SAC1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2292</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REW7">
        <property type="interactions" value="1361"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REW7"/>
    <dbReference type="InParanoid" id="A0A1E5REW7"/>
    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034593">
        <property type="term" value="F:phosphatidylinositol bisphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043812">
        <property type="term" value="F:phosphatidylinositol-4-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662">
        <property type="entry name" value="PHOSPHATIDYLINOSITIDE PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662:SF2">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3-PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="536"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="569"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="SAC" evidence="2">
        <location>
            <begin position="114"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="636" mass="73679" checksum="F58EB3AD8F863FF4" modified="2017-01-18" version="1">MSFELAETKDGIFIKKNDTVLNFSSKYYSSSGLFSTDQKFPSNGNIEKFACLIGCIELKINKYIIIATKTENTGTIRSFDSVSHEINKITDFRIIPINKQILPTREESQYLKLLNDHLSKATLYFSPTYDLTNSSQRQYDMLATXNSNEPSFDKCDKRFFWNYYVTKNLRELSVSQPSYASFVTPIIYGYSKFVDSTYNNHSIKLGLITRRSVYRAGTRYFRRGVDXFGNVGNFNETEQILVVDNNQYYSFLQTRGSVPVQWCEVNNLKYKPQLFVNDSVSMEHTKKHFDQQVELYGDNYLVNLVNQKGYEMPVKKGYEDAVKFLHNPKLHYIYFDFHHECRKMQWHRVHLLIDELVKLGLHNNNYFHYQMSSSSEGKLISKQTAIVRTNCMDCLDRTNVVQSVLASWVLQNQLEFSDVVSHEKILESNTNEKPAYKSWNEENPDLLFEFQNFWADNADSVSCAYSGTGALKTDFTRTGKRTYAGALQDLKNSISRYYQNNLTDGPRQDGYDLFLGNFKPFTDAVQNPFSDRRPSYIQVVPSVIYFSVLVFVATVLFPRNSSFKSLQNLTFFFGSVASIAFSLKYIMKNGMQYVNWPKLCDVGFLEVEEHATTDNQFQGMKFNVNPQFLTGFNKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF42</accession>
    <name>A0A1E5RF42_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g2244</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RF42">
        <property type="interactions" value="461"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF42"/>
    <dbReference type="InParanoid" id="A0A1E5RF42"/>
    <dbReference type="OrthoDB" id="10252171at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045719">
        <property type="term" value="P:negative regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060917">
        <property type="term" value="P:regulation of (1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14004">
        <property type="entry name" value="STKc_PASK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000320">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000314">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF51">
        <property type="entry name" value="PAS DOMAIN-CONTAINING SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="1075"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="320"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="914"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="958"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="174"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="227"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="327"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="919"/>
            <end position="930"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ85520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1335" mass="151496" checksum="E3EED03DB94BB1F6" modified="2017-01-18" version="1">MFNTNIFTNEKHVGNKLKTNTIKTSEDGACSKNTSRVLPKTRKKRHSIPTFDIQEELQQPRSLETQHIPQEEVVLEEQPIHSNLNQTFVSQNNTLLAQEDVFMKELISFPSLSTHSFSYNPLSPNSLFVRLIILKRTLEVMLHNPSLIKDPTETHVAKNKISDLKEEARPNMIVSRSTNDLYRSPGYQNFSPMRRKISQQLIQDPSATSKRNASSAAFDAYVKEPTSASSHSGNNSNRSSIIAPSVSSHNLSRVLSSQSGSEDEQELDFAQPIAPFLKRHYSAGLTSHGNGHNKLESLFAGSNVHNNAHIKTGLVKNDFSKGRDSITEENDENPDSINDYEDYNDLLSSYSSDSDNHSVATNLTRTPFDTHERKSSFYKLEDFQDSSEYLQTKTHDLQSLLDLLNETLENNVVDLPATENEKQVSNLHMMSVLNITKLLASSSSFQKESERSENLRKNLIDSLSQPFYELSHRAEQDYSQRILHDFTAGKNSSPQAILLCENENPWSFKNVNDIACLMFGIARNSFRALNLLDLIHKDSRNFVLNKLVVTELENDNLVFTGEIIGIAQPSVKEPVWTSLWARRKNGLIHFLFQKVPCDSIKCDFSLQHLKVDNFSADKAITFRVKTTYRDESETTEEGKNAIKKVKSVHDIEDLFEISNSISEFISNIKKTGYEDHEMNVAEIIKGINDTRYFTLNSLDYNIPCAVTAGYAFXSDIKLNIHSMPYIAGAFIIDSSTSSYELISFNKSVSKNLFGIHAKNLMKKPIKTVLPRFNAIIEHIHRYYPEYNINKETNKSLVLTEHFFRKVNTELEVYANLITPQEYESKFYNSKGINGMHRDGSLIKLDVQLRVLNTKYALLWITHSRDIVSKNYNIMPSQMKILKENEMFLSHQHNEMDEGLVSNDTEYDRSIKEKLENVKXSGXSGGSSTTTLSEKNSRGPSAKSLTKLLTSLSGKRSDTTIFNERTVSGKENDDPDESQTHQQAEFLSDEESPVDIAKKYKQNKESFLKSSNFNFDRQLIISATSRPSSANTVEAISKVPEANGHVASYANLYKNVDGMETFKLGQLKHKKKYTDFXLLESMGRGAYGLVDLCMHKKLKYIIVLKRIIKERILFDTWVRDVRLGTVPSEIHIMALLNDSPHENIMYLLDFFEDDDFYYIETPIHGNTGSIDLFDLIEVKTDLKEIEVKLLFKQVVSGVKHLHALGIVHRDIKDENVIVDGNGCIKIIDFGAATHVAKGPFDTFVGTIDYAAPEVLSGEKYVGKPQDCWSLGVLLYTIVFKENPFYNIDEIMDAELKIPTGTNVSNECINLIKKILDKNVNQRPDINTIFNDPWLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF74</accession>
    <name>A0A1E5RF74_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor eIF2B subunit delta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">eIF2B GDP-GTP exchange factor subunit delta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2272</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF74"/>
    <dbReference type="FunCoup" id="A0A1E5RF74">
        <property type="interactions" value="834"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF74"/>
    <dbReference type="InParanoid" id="A0A1E5RF74"/>
    <dbReference type="OrthoDB" id="10254737at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10233">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10233:SF14">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="522"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="528"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="542"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="610" mass="67591" checksum="00AE370411F0F78E" modified="2017-01-18" version="1">MSETMSNSVQEKKEAVENSAGSNETNKPSPSGAADKDATSSKESSEGSGGLSNKEMKELKKQEKAAKRAAKKAAASGISIEKQQQNAELKKEKKQKQKEAELKKEQEKEQKIKERKYGAAGGAGLASKSTLFGHLETAAERKASLLAVNAVISSTKQTKITSQGLSLQSSIFNSSPELVGQPQPDSVGVPGTCSIIPDTILKEVSGSSELTQQIKDLIINKDMLHKATLKLTDDFAKYNIVGSTPRCLAMLEVFKQIISDYQTPPGTILSRNLTQYLSHQIDFITKARPLSVTMGNTIRWLKQQISMLDVDVKEKDAKQLLLESIDMFTQEKIKLADRVIVESASQHIQNNSTIVTYGCSEVLKELFVHNKVNLKKDFKVIIVDSRPLHEGKKCADYLKDQGLDVMYCMITSLAYVFQTIDIDYVIMGAHSILSNGFMYSRVGSAMLAMTAKLKNIPVIICCETLKFTNRVQLDSLTFNELADPNDLLGVETEYKDKDEFKNVLLEQFIKDKKQFLQSKAAIAESGSHKNHGQKANIKNKHNAKDKSEKSVSTKQKNASSILEKWETNPNFNIFNTMYDLTPPEYISKIITESGALPPSSVPVIIREYNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFG7</accession>
    <name>A0A1E5RFG7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">YAP1-binding protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1778</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFG7">
        <property type="interactions" value="78"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFG7"/>
    <dbReference type="InParanoid" id="A0A1E5RFG7"/>
    <dbReference type="OrthoDB" id="5396786at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013877">
        <property type="entry name" value="YAP-bd/ALF4/Glomulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040347">
        <property type="entry name" value="YBP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28020">
        <property type="entry name" value="YAP1-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28020:SF1">
        <property type="entry name" value="YAP1-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08568">
        <property type="entry name" value="Kinetochor_Ybp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="660" mass="76193" checksum="84D7D825907D0294" modified="2017-01-18" version="1">MSTEEKATKPVDAQNDNDHTTDSETSTTPPLSVEQICSNLKSAFKETDPLTLNTMIELYSNQLIEANGKGDAVKFLESLHEELMAANEELLDAIGWDLPKVLLEFLRLQYMNLELYYVDNMTVPLVMGCFSELASRGNAKDLFLMGCELLSEVSYEVEIANLPDFHXSQRTSELIDRHPAECIVEVKMHILTELINWCLKNITTQFPSKFLSMAVNSFLTYIKGNIKSIFDCSFILRRVYTFVRGYIPPRPTDPSDFEEEAEGLLQRKLLIFLLTTGLAECLQSPQTAIYKFENYYYTKEILKKDYKVIERLQKFLEIYERIYDLALSFDLDIEQEFAKHCIEESCTIYENLENDSSEYILKLPAVYDIVKKSKEKEITLDYRGVLILAGYMYHVKKAKYPENDTFKLSKLVYLFIRVFSLDKVAQSINHTTFVLQGTVCNFMWYPLFKYSYIENQKELEKLPSAILQTLYHLIFLKISSTKDKQEKIIYSTLLTRVLCVSPEKEVYSLAFDTFSSCPLSEIKCILISILKDLMVRTVDTTSLCEQLQKLDVSGTTVKEEQPNKAPALPPRTFLKVTPDNMNKFHAHTFTLIDEIKAEPNADNLTELLAFINFYIRLKSLWNKEHIESIKTKITDELVESEDFNCNLPELTFIQISLKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFH1</accession>
    <name>A0A1E5RFH1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2077</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFH1">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RFH1"/>
    <dbReference type="OrthoDB" id="4069826at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040939">
        <property type="entry name" value="Vps38"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17649">
        <property type="entry name" value="VPS38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="408"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="277"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="3">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="439" mass="50997" checksum="3D42DBD8355F0C92" modified="2017-01-18" version="1">MISKLKKRNISRPLNSHTLLTSMAFPIARKLRHLKCVRFENIPLAASLSSIEWFYTIELVPGNQLIYISENSPVTLDTATRSIESSDHLNDLNNNRSLLTIEFDQLPEFTYNNHNVKISLYACVENVNCNSDGDDERSNHSLERSSSETVSVKIKELTLCLDANLRLKKNVLKTNNSTNAFYPTFQFDDLXWYVYVPETLSQERDETHSLESIDDAAIELAKFAYTNRKSYSFNALLKLNKLLEYHYELDQEISNLKDEIDVNYQLPEQPYKKTHPVFQIQSAIDQLELDVQKQQNDKAKQEGRNDKLNFIKISLQEKLKENVDFLLPDDSNDMIKDYKRRKQQLTELKIRKIQKLLNIFESCNVVSGLYPKTSVKKQYPTLAVLQPNDLLLESQTNDKKRLLLNSKLVFSFMVVLLWWNTITPFTLCPQKANKMRASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGE1</accession>
    <name>A0A1E5RGE1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">General transcriptional corepressor CYC8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2184</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CYC8/SSN6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGE1"/>
    <dbReference type="InParanoid" id="A0A1E5RGE1"/>
    <dbReference type="OrthoDB" id="418911at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017053">
        <property type="term" value="C:transcription repressor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000403">
        <property type="entry name" value="General transcriptional repressor, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000078">
        <property type="entry name" value="Transcriptional corepressor Cyc8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051630">
        <property type="entry name" value="Corepressor-Demethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14017:SF1">
        <property type="entry name" value="LD02225P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14017">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13176">
        <property type="entry name" value="TPR_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4 9" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="113"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="147"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="181"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="217"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="254"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="291"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="363"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="397"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="477"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="504"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="550"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="634"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="821"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="477"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="550"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="591"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="641"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="677"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="687"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="726"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="737"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="824"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="842"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="878"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="921"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="932"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1079"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1115"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1236"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1254"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1266"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1280"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="11">
        <location>
            <position position="972"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1324" mass="143351" checksum="905B04F268AD4271" modified="2017-01-18" version="1">MNATTALPGMGAMTHQPPAVHNNGNPLDTNNQVQGQNQNDNSLAAVQSLSQAASIAAQQQQQQLGLQXQQQQQQQQQQQQQLVPMGQMGSMXPALPQVNVSMVALDPLSQSTAETWLSIASLAETMGDLDRAAIAYDYTLHHNPNSTKALTNLANLYKQRDMFQRAADLYQRTLAINPQLGDCWSSLGHCYLMLDDLQRAYVAFQQALYYLTNPNIPKLWHGIGILYDRYGSLDYAEEAFAKVLELDPNFEKNNEIYFRLGIIYKHQNKLQQSLECFRYILPNPPQPLQEYDIWFQLGLVLENMGEYQSSKEAYETVLLKNPQHPKALQCLGCLYALNQVSFYDHDLALTYLSKSLEYDSTDSATWYHLGRIHMVKQDYTAAYESFQHAVNRDSRNPTFWCSIGVLYFTIGQYRDALDAYTRAIRLNPYISEVWYDLGTLYETCNNQINDALDAYRQAARLDPNNVHIRERLEILTQQQQQPGGISQQQGTLNQPPTLLHQQNMQNQQPHNGQHQQQHQQQQQQQQQQQPVMLQPALPQQEIINPLNSSYQQQLPQSMQQPLHQQSAPPQPPLIPNGSSSIVPQKRALDDSSYNQANQQKQHAPLNPGLQYQQPQHSNMDSLITAATSIHSTPTAGTAALNSNTVTGANNTNNNTAPASGTSTANGTPTGMPPVPVKGKRGRKKLQKNIDKEREKMLQKENAKKNHEQNGQKTGPKQTRKNKEAKAAAAAAAAAAASGTTTGTPVADNVTTPVSDSGNLHVPPDSAVKHEPVAEQPQPNGSAHILPAPPVFQQQQQQQQEMPAPSHVLPALQNPLQLAPXHSVQHASQQTPQEVAQPQVMHSSASSSSLTPXSFPAMQQAPVLPQSNSATVAPPPPQQQQQQQQQQQQQQLETVNKSPVAAPVASIQQVEEPKPVAAQETSAPSAVSASSALQTPNETVNGANTLAQPQPDVAATESKXVESATVNPXSVPDQXXXVVHSESQPAALVSAQPVETQPAAVLPTSEIETQEQKAALGNGEAIPVAEPVAFTNNTTPAPELVPPSTEXTAVQTPFXATSAPAPAPVPASTEHATPLAAPEPAVSSQPALAAPAQPAETPAAFTEPAAATAVVNPVTQQETTPAAEPVTQQETTFAAEPVTQQETTTAAEPVTHQETTPAAEPVTQQETTPAAEPVTQQETTPAAEPVTQQETASAPKSEPANPENVVEAPSVDISQPEAVEQPTTVIKPSEETLAQLEEAKKIKEEEERERAQAATTNSLNTAVSPINATTNGTATSTGATPFSSSNASIQPLSTEQNASAAKTDAPTADEPHVVREVEEDENYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGP3</accession>
    <name>A0A1E5RGP3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1590</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGP3"/>
    <dbReference type="FunCoup" id="A0A1E5RGP3">
        <property type="interactions" value="43"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGP3"/>
    <dbReference type="InParanoid" id="A0A1E5RGP3"/>
    <dbReference type="OrthoDB" id="4067115at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="64"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="170"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="204" mass="23438" checksum="514DFA5BB83546AB" modified="2017-01-18" version="1">MIPQAHYDVALLREHAYYENYDLNIVLDHRTYTAEGGYKPIRDYGLGYFNYPLALSKQILGKNILYGFSMFVYNYFTLFLMLYLVLLIFATNSSDKFAFPSSNNKHNASIGALSQTVSQWFDFRSISNKLKIGSSKNKLKKFDSMNDSDVDHQERGELHLNNVNGVSIHTNNSNNSSSTTSRGSTTTYRDEHENDDLEYQHVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGP9</accession>
    <name>A0A1E5RGP9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1558</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGP9"/>
    <dbReference type="InParanoid" id="A0A1E5RGP9"/>
    <dbReference type="OrthoDB" id="3367at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="531" mass="59547" checksum="52FF72B7011B5BFB" modified="2017-01-18" version="1">MVQERKTYSKLPSTARKSYGSRFNSNAEPVASSRENKNRFARETKKNGPNIYLGSSTSASNIPSNEQARNRESSNSAALSRNSDREAEARRNRSNLSSSSSSSSSSYRPSTSTNAPAQAAPVSHLSERKTSLTQRSTTGLRSLPTEPSALRKRNERASLLEYTKLSKNRGEKDNIANPNLRTKREKTCHVYSHRLVTHCLVKGGERRRSKWGRNSRQVEPPSGLPYVLDLDAGSQFLAYISDDRKLLIGELTNSMESLSRIDKRGTSLIKYGTTRTRGFSWSRRYPGVFGIITTEQAFGNPEKHFIKLFEKTTHTGLQQIKSVEVEQPLIDCVFSPDNRYLAVLLEDNKLTLYSSDLEILHQTIDVHGNCTSINWHPSAKLLHLGTDAGECLIYSEESPESWILKTKMDISHCAVTNIVCDTRGQLVLFASSDGNLQVFSLEDYTKINSFESEKGYAILNIDLSSDGHVVYISYVDEDTKEVYVCFYHWPTNTKIHEEANFSFAKATNQKVGFLSVSQGAIYSHLLPPLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGQ9</accession>
    <name>A0A1E5RGQ9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TEL2-interacting protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1149</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TTI2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGQ9">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGQ9"/>
    <dbReference type="InParanoid" id="A0A1E5RGQ9"/>
    <dbReference type="OrthoDB" id="6417021at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110078">
        <property type="term" value="C:TTT Hsp90 cochaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018870">
        <property type="entry name" value="Tti2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10521">
        <property type="entry name" value="Tti2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="350" mass="40738" checksum="F5D4D6985C6AE706" modified="2017-01-18" version="1">MASIKELEQWVLFGETLEFVHEITVDDKLIILWIKEQLPKTTTSNRSITPAGRLKASKLWNNALLPKIGLSLLDDDQEASLSIMNHRMQWLVLRTIDQFSNSISQELWWITPTILAFLDHSKISVKLLGVEMLSHFLXSVPQQVFVNAGLFTVYYEALRNLCFYLPPAVDQPSVLKIMQKVYPCMVALISKSVHPENHFLEFFSDIILKATIPRIYDLGPQLSVYVLKFLRTEYLTKRKNEVFINVQRLIYVIGNYFVQNPFLTTDISLLQETQKILKVLLXLDSQYKYDYLAMIIIMWEKCMKEGQADQHETLHKLYLELSKKAXFTQEERTALIDSRQDLDLKVLLTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGR3</accession>
    <name>A0A1E5RGR3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PWWP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1365</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGR3"/>
    <dbReference type="InParanoid" id="A0A1E5RGR3"/>
    <dbReference type="OrthoDB" id="62853at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05840">
        <property type="entry name" value="PWWP_ScIOC4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035503">
        <property type="entry name" value="IOC4-like_PWWP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWWP" evidence="2">
        <location>
            <begin position="3"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="233"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="427" mass="48613" checksum="48E8DA07CC0C1FC3" modified="2017-01-18" version="1">MSNEKSILVKVKGYPSWPALVIPTEKIPKRLLGSKTTSPENHYCVKFYNDNDYIWLKKSKTMSLAKQYDLLDSAEKITALIDKEASKRKTNDKLIKAYQMVLEQVNGVFAVDEFLXAGEEKYIDESTNEQDLEQQQQQQQQQQQQQQQQQDNTKDNKNEKNEXAPTIEKETGKTETDKKKKPVASKKRTIKQENEDQPVKKQDTKAKKQPAKKATAAVKQESSKEATPKPSKKAATPTATKKSSTKKAATPAAAAAAAEKKTPSKKTFKFESPLVTGTTIPIYELVNRGPQHLDILKITPSNCGVASLPNFAVLSEKKLDLQDAMQXLKNLLFGFFTEYIKLKKKDKDVETIMAEPEFVEKSNAILAEFENLANNKSVNYFKYLFLQDQEFYHYFSTYLNVWKSTKSSDNSAERSEMKTLFEKFYVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH28</accession>
    <name>A0A1E5RH28_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nucleoporin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1338</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH28">
        <property type="interactions" value="168"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RH28"/>
    <dbReference type="OrthoDB" id="67716at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059141">
        <property type="entry name" value="Beta-prop_Nup120_160"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055090">
        <property type="entry name" value="NUP120_helical_saccharomycetes"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11715">
        <property type="entry name" value="Beta-prop_Nup120_160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22114">
        <property type="entry name" value="NUP120_helical_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoporin Nup120/160 beta-propeller" evidence="1">
        <location>
            <begin position="101"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin NUP120 helical" evidence="2">
        <location>
            <begin position="505"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1010" mass="116303" checksum="5D30923D1AE6D5D6" modified="2017-01-18" version="1">MTGPLVKLDFDLPKSKDIQHVFLHSDDLVNQSLNGAASGVASNKNANVGSYDYANTYAVKRDNQRDTAQAAPATXGYHLVNNYQGLLMYHFETKIDTISPLLLHFPDQLYNKSFKSIDIRNNDDLLLIDXILQNGMIICMSVPFDFKFTDKKFFKTFFPFDFTFREPYQLCSIDNTFQIITLKDGGILGLIKHYGPESAILPEDYETILFDKVSYLEXLSKKLFAFGNGANKKRKLNNDCIVSVQYLESSNQLLTISNDLVLKLWSIQSADTAFNIQVAQEINFLEAFNDLGEHDIHSSGSPLSAITDDLKHLVVYLPIKSGIFQIYNLDAEKGSIVKNAEFGSTMEYGSIWHLVDLKILEKTQEVICLWKGNMDGLVQVFKNGKWTDCDNTSFQDSMQDVYLKSENDLAYSVMNLRCHYSSIVKNKLKPYEDGLXRLDVDALKHMDVQFKHAKKVRDEPCSLMLKNNSMIIVNNLSIYSHSLYVPKQFAISLEKQTSSIKKITGVVEDFAACISDDIFHDLSAKFLAKIVENKFQDFAIEDLEDFKQCVSAERLENLVAGLSNLDNFTKDLQSVISSILEETADKGTDLLTVQNYDNMSLLENLFYKISIVDRLVLKFLAAFSVLEFDYSTIMKEIKSLLRLHYVSLLFLTLYKWDKSFLLAELMKHYSLFGKGFLVQNYEQLNDCTIFLSNKILNDDGFGDVFMTIYVSMLDRGLXSTENSKLIEFSKFIAQPFSLITNEKQQFFLGLMHYNTHKYQESFDILFKQSEKWETLPSKYIKTPEESVHLEDFAELIQASNDAQYCYELSKKYSQAEQYNLSLQSVKRSIELYPVTLDDEHDPDLKKLKYNQTVQYLITLNHFHLYKEILEVLINSNDVLNHDIKYDYYSNFLLENQQFIDEIIKYSFSKEVVLPFQDYDIVDKALSNMLDLTNFKTYLILYSYRIANENSRSAVESLYEYIVNANKNGSNYQENQRAAKTLLINVLNSFTRDDDKWLTTKDGLVVLLKDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHI5</accession>
    <name>A0A1E5RHI5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Eukaryotic translation initiation factor eIF-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1547</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Additional factor that functions in concert with eIF-2 and the initiator tRNA in directing the ribosome to the proper start site of translation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SUI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHI5"/>
    <dbReference type="FunCoup" id="A0A1E5RHI5">
        <property type="interactions" value="749"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHI5"/>
    <dbReference type="InParanoid" id="A0A1E5RHI5"/>
    <dbReference type="OrthoDB" id="10248435at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11566">
        <property type="entry name" value="eIF1_SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.780.10:FF:000005">
        <property type="entry name" value="Sui1 translation initiation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005874">
        <property type="entry name" value="SUI1_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01160">
        <property type="entry name" value="SUI1_MOF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10388">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004499">
        <property type="entry name" value="SUI1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="SUI1" evidence="6">
        <location>
            <begin position="27"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00060093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="109" mass="12431" checksum="FA3467534E8F59C8" modified="2017-01-18" version="1">MASIENLKSFDPFSDTGDDDSTITNYIHIRIQQRNGRKTLTTVQGVPAEYDLKRILKVLKKDFACNGNIVKDEEMGEIIQLQGDQRSKVCEFMITQLGLKKKNIKIHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHK3</accession>
    <name>A0A1E5RHK3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cell division control protein 73</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1237</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDC73 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHK3">
        <property type="interactions" value="235"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHK3"/>
    <dbReference type="InParanoid" id="A0A1E5RHK3"/>
    <dbReference type="OrthoDB" id="2186602at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11990">
        <property type="entry name" value="RNA polymerase II accessory factor, Cdc73 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007852">
        <property type="entry name" value="Cdc73/Parafibromin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031336">
        <property type="entry name" value="CDC73_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038103">
        <property type="entry name" value="CDC73_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12466">
        <property type="entry name" value="CDC73 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12466:SF8">
        <property type="entry name" value="PARAFIBROMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05179">
        <property type="entry name" value="CDC73_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="Cell division control protein 73 C-terminal" evidence="6">
        <location>
            <begin position="218"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="121"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="133"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="376" mass="43642" checksum="289A1F68C770EEBB" modified="2017-01-18" version="1">MSEALXKLRDAIKQNAQFELQKEDGSISDDIATTDLLKISGPDVNDVLKLEEKTNFGNNFTLRAIYHCWLNKDSSAADYLKECESKNIPNISFLQRTDLYNWLTGVSSELKYVKSTDSSKDSNESIVQHLEPRTNSHNNQNDFADSESQEVLNNERILLDHNSALRGSKPINFHYLIKQVDQTLLKQLKEELKNKNFINKKRPASNTSFAQGETKXKQKEPIIMIPSAASSLFTMANIKQFLVDGVYISPRDLPPSSQDMIRIEKKLDSIEKPLRFIIVNNTRLFSKQEYWTRVIGVFVSGHEWQFSHYKWSKPQELFQHCNGYFFRFDGDSVPKTVSSWNVRELTLDKTKRFRDLETSRLFWNSVQKELNVRGYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHN5</accession>
    <name>A0A1E5RHN5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">WD repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1455</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHN5">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHN5"/>
    <dbReference type="OrthoDB" id="1932312at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040324">
        <property type="entry name" value="WDR44/Dgr2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14221">
        <property type="entry name" value="WD REPEAT DOMAIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14221:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="322"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="27"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="252"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="569"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="54"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="174"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="253"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="269"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="569"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ86411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="897" mass="100040" checksum="E29B03F8E43741E8" modified="2017-01-18" version="1">MSDLQKNSLLGDGIISIPLQVTFADGKNEEHKKAXTPHNAETAREQKETDNMSDESLSNASIKDGKNNTGEIESLEHSVINMNFKSXSTPKRKTSFQLHEPQVEPYCKDQPKNEIGKNQRLDSLFESIDIPTYIEHLQNPEQIKIYKKRKHLKLKRPLVLVQELDDNMHASATPKXNGTVTPVSANFSDSGSSKKKNTVNADCPNAVWCLEFSKDGLYMATGAKNGVCTIWKVFSSALERLKVKNGEFRSKDSQYTSAIQRKNSTSTESLPSMYSFHSSSPSPESIEESTKTSHTTSDGKLRDKTPVGSYSPIFLPQPFRIYDAHSADILDMNWSKNNFLLTASQDRLVKLWHVDKRESLKQYLHPDFVTGVKFHPADDRFFISGCLDHYVRFFSILDDEVRYEFDCNDLVTCLECSPGDGKYTVVGTFNGYILVFLTEELTLLACLHVSKHEDLEVIKCEENLDTDMKPSNAGGRVIDHISPGPKITSVQFFKDQNGILKLIISSNDCKVRVFDFAERKTVEILKGIKEKFVQLKATASINMEGNAFVIASSEDQWLHGWALQTSNSMKARGTPKTSTNDQRSLLYSGKTRSGSLSTSIFSQNDRNSNNALMKSKNEEPGLVKKLFNVITNKKQTNKTVKGLPSQNYTLNKKHSHLPGLHTSTNTHLFGTQTFKNSTMMSFHAHKRPITCATVVPKATENTLSLSNDFLYELSEMYGLQAESYLEQEDKPIRKARLHXSGSAXSLASTTAIQAPSLNRSDSNESSEYGVLTSITAGAIVVSADCNGKIRVFRKDLPDTVRKDVLNALIMTQHKNENAYINPXSGXLQVQCPSQTLPPDRCLLAPNGSQKPSRKNTLKRLTSISSKGNKNSTCSLHTLMDSVNSCSLQNSPMQLNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHP8</accession>
    <name>A0A1E5RHP8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome b-c1 complex subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Complex III subunit 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1493</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The complex plays an important role in the uptake of multiple carbon sources present in different host niches.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein, 2 core protein subunits, and additional low-molecular weight protein subunits. The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with cytochrome c oxidase (complex IV, CIV).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion inner membrane</location>
            <topology evidence="1 11">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the UQCRQ/QCR8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHP8"/>
    <dbReference type="FunCoup" id="A0A1E5RHP8">
        <property type="interactions" value="94"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHP8"/>
    <dbReference type="InParanoid" id="A0A1E5RHP8"/>
    <dbReference type="OrthoDB" id="6683853at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.210:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.210">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004205">
        <property type="entry name" value="Cyt_bc1_su8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036642">
        <property type="entry name" value="Cyt_bc1_su8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12119:SF2">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12119">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX UBIQUINONE-BINDING PROTEIN QP-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02939">
        <property type="entry name" value="UcrQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81508">
        <property type="entry name" value="Ubiquinone-binding protein QP-C of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ86103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="94" mass="10658" checksum="6B68ACD062BC294D" modified="2017-01-18" version="1">MGGPSAKTYMGWWGHMGGPKQKGITSYAVSPYAQKPLGGIFHAAIFNVTRRVKSQVLYIVLPAAMYWVWWTNAEKYNSFLYTKAGRKELERVNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHW3</accession>
    <name>A0A1E5RHW3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EKC/KEOPS complex subunit BUD32</fullName>
            <ecNumber evidence="6">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24 25">Atypical Serine/threonine protein kinase BUD32</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">EKC/KEOPS complex subunit bud32</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="ORF">AWRI3579_g1039</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the EKC/KEOPS complex composed of at least BUD32, CGI121, GON7, KAE1 and PCC1; the whole complex dimerizes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. BUD32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="29">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHW3"/>
    <dbReference type="FunCoup" id="A0A1E5RHW3">
        <property type="interactions" value="874"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHW3"/>
    <dbReference type="InParanoid" id="A0A1E5RHW3"/>
    <dbReference type="OrthoDB" id="3399at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070525">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000745">
        <property type="entry name" value="Serine/threonine-protein kinase BUD32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022495">
        <property type="entry name" value="Bud32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03724">
        <property type="entry name" value="arch_bud32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209:SF0">
        <property type="entry name" value="EKC_KEOPS COMPLEX SUBUNIT TP53RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209">
        <property type="entry name" value="NON-SPECIFIC SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="21" id="KW-0010">Activator</keyword>
    <keyword evidence="18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="19" id="KW-0779">Telomere</keyword>
    <keyword evidence="22" id="KW-0804">Transcription</keyword>
    <keyword evidence="20" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Protein kinase" evidence="28">
        <location>
            <begin position="15"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00019973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00030980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00033194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="EMBL" id="OEJ86480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="259" mass="29422" checksum="FEFDEAF49FEADA39" modified="2017-01-18" version="1">MDEIVTGVEHYLTENVPVKLISQGAEALVFETSVHPYNPNKTDETYIIKYRPQKAYRHPQIDKTLTKHRTLGESRCLAKLATMSGINAPQLIACDPYNGYIWQSKIGGLLPNGEFSNLKNYLWFNKNDPYSTINVENVLISVGKQIGILHCNQYVHGDLTSSNIVLEKSGLNNWIPFLIDFGLSSYSTLVEDKGVDLYVLERAILSTHSSFAENYNKWLLKGYESAYNEFYGAKSSELVQVLKKFEEVRLRGRKRSMIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI11</accession>
    <name>A0A1E5RI11_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LisH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1632</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI11"/>
    <dbReference type="STRING" id="56408.A0A1E5RI11"/>
    <dbReference type="InParanoid" id="A0A1E5RI11"/>
    <dbReference type="OrthoDB" id="972532at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034657">
        <property type="term" value="C:GID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051350">
        <property type="entry name" value="WD_repeat-ST_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22838">
        <property type="entry name" value="WD REPEAT PROTEIN 26-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22838:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="849" mass="98336" checksum="D6E5C9ED67C6E776" modified="2017-01-18" version="1">MNKDQLIRVLYHTLRENGYDRTCNELLRETETATAQKIHQTEYLQAEIFNKFTKTNGDGINCLNDLFQLNFGMILGCKLKNRGGDSKNNREVNNNLINAYKSYDYKQIQVSKKPPYRDLLNQQNHQLVDHLFEFLKIFEKDFSFIQKYIININVQGPYVEQEFVEMYADILCFIFYKLVRITYYQENNGVVQKDKQSSLLPFMFFQHVLLVLQQIPFLSKFANTTNNNNNNNNNAQSAVLLSMSPGDALMYRNKYFQYDIIEPKDGDYFVKYLVLKYLENDIFVPKNYLESLLSSSLSKTSTSNDILQYPMVKKSIETNNQPVPNINNITIDINLKGMTKTNIFDYFLQSQKKNKSFSKTVSFKSTKNNFLQKTPVSSGSCVFSNNGKLVLLTYRVDVQDEDDTKSNITVDKYTDRRSNIELLYYDEFQNKCEKITSFNTQYKESFPLGIAKKMEWYSEYFTKVLFSPDNRYLGLFSNIGTLYIYQIDYQELQNYYRQKYENDPGAEKIEAENTKTFVTGFLKMFYNKNEDEIGSTNFNDDVSMDAENTTGTKQNMEIQVDGVYSCFDWLDNKHVVMGSLNNNNMLIVDVEKKKIVFNNNKSHKKCISPVPIEKIFVLNTHSTSNKIHKKLIIVSPSSILLENFNNISYKSTPQPDTQTIVFENSNAEMETGKTTGKTKTETQFQKPTTAVLCYDIVQPVEQYLHNLRSKPTADFLFFKPTNIFQINAVVTSVAFPTSVDESLPYCLVNTIDDEVQVWDYSIGVRVAKYYGAENYQQIKIVPTFLGENSEFVLCGSEQGKLLIWNRYNGNFIGVIETDQGTTTAVLANPVYKNHFISFHDNTAFCDWSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI26</accession>
    <name>A0A1E5RI26_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Golgi reassembly-stacking protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1635</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI26"/>
    <dbReference type="FunCoup" id="A0A1E5RI26">
        <property type="interactions" value="112"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI26"/>
    <dbReference type="InParanoid" id="A0A1E5RI26"/>
    <dbReference type="OrthoDB" id="3318at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007583">
        <property type="entry name" value="GRASP55_65"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024958">
        <property type="entry name" value="GRASP_PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12893">
        <property type="entry name" value="GOLGI REASSEMBLY STACKING PROTEIN GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12893:SF0">
        <property type="entry name" value="GRASP65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04495">
        <property type="entry name" value="GRASP55_65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51865">
        <property type="entry name" value="PDZ_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDZ GRASP-type" evidence="6">
        <location>
            <begin position="187"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="300"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="362"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="300"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="372"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="391"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="409"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="425" mass="46981" checksum="27943ED779BD2FFF" modified="2017-01-18" version="1">MFNIAKKLVKTFEQSVSETLNTNGSASATNDSYFQSIPPNLLQYPNEYRPTIDTLHGLRVVQCDDTQLQLHTFFDYIVGINDEPLPIYFNTHGWPFPNYNEVYRILNNCLLNGSDTSSKYVKLNVWSGKGGFYRDEYIQLTQLSGMQDISLDDENTTEQAGPNVVVKPLFESLGFQIQWQPLIGSTFTYHILSITDNYGTAARVGLIPQKDYIIGCQDGLLSTGGETLLQDILQAKCNQDLVLYVYNSDKDFVRPITVSLNGTGRLGCNVGYGYLHRIPVPAAILSTENPDIINPIAQTMPQQQHPQQQEQLSDLPPLDPSSTFQPMQFQQTIIAPVPTTFAPKKKKHSQHTAAHASTNLLNDLQEKEPQPTYSSNTTSSATTASDDVSPSAPPTIAPPPTSSTKKATESVTPLSTSTESQEEPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIV9</accession>
    <name>A0A1E5RIV9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Glutaredoxin-2, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1627</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>1-chloro-2,4-dinitrobenzene + glutathione = 2,4-dinitrophenyl-S-glutathione + chloride + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51220"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:34718"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:133977"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 glutathione + H2O2 = glutathione disulfide + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:16833"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="EC" id="1.11.1.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RIV9"/>
    <dbReference type="FunCoup" id="A0A1E5RIV9">
        <property type="interactions" value="700"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RIV9"/>
    <dbReference type="InParanoid" id="A0A1E5RIV9"/>
    <dbReference type="OrthoDB" id="418495at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015038">
        <property type="term" value="F:glutathione disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03419">
        <property type="entry name" value="GRX_GRXh_1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000026">
        <property type="entry name" value="Glutaredoxin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011767">
        <property type="entry name" value="GLR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011899">
        <property type="entry name" value="Glutaredoxin_euk/vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014025">
        <property type="entry name" value="Glutaredoxin_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02180">
        <property type="entry name" value="GRX_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694">
        <property type="entry name" value="GLUTAREDOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694:SF18">
        <property type="entry name" value="GLUTAREDOXIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00160">
        <property type="entry name" value="GLUTAREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00195">
        <property type="entry name" value="GLUTAREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="8">
        <location>
            <begin position="61"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="151" mass="16711" checksum="1C5B8C6969EB982D" modified="2017-01-18" version="1">MFELLDIKYLAVSVIVIAILKNVLLKEQKTTTKTRISNTQSEMVSAAVIQNVKKAISSSKIFVAHKSYCPYCHATLNTLLKDKKIPESKINLLELDTMKDGAEVQDALAEISGQRTVPNIYILGKHIGGNSELQQLDKEGKLDELLKEALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RJ30</accession>
    <name>A0A1E5RJ30_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Grand meiotic recombination cluster protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1196</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RJ30"/>
    <dbReference type="OrthoDB" id="4067025at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="189" mass="21104" checksum="2B1A33B8E13235DC" modified="2017-01-18" version="1">MTEASVKNIDTSSHLGTSMPLKEATTQSKVTEVXNALDTSIPLKDSVMAYLDGLKGRVDVLQVTKXXSKEAVLIRFKEATDILQVFDSKSGTKTGSVNWDELYSFSANIMDEYTQEIDQLLKELDTLYKDQFIWQEAASTVDSRRAASQIAQAEEWIVQKEANIQHMNLEFLESADIIKKTIQGLGSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN02</accession>
    <name>A0A1E5RN02_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">54S ribosomal protein L22, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g654</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN02"/>
    <dbReference type="FunCoup" id="A0A1E5RN02">
        <property type="interactions" value="317"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN02"/>
    <dbReference type="InParanoid" id="A0A1E5RN02"/>
    <dbReference type="OrthoDB" id="416470at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="entry name" value="Ribosomal protein L22/L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_uL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047867">
        <property type="entry name" value="Ribosomal_uL22_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_uL22_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13501">
        <property type="entry name" value="CHLOROPLAST 50S RIBOSOMAL PROTEIN L22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13501:SF8">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL22M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="Ribosomal protein L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ88264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="314" mass="35951" checksum="1864E9FA70BAA368" modified="2017-01-18" version="1">MYRVVRRFATSSISRNSASSASGAGNLFGEFTKTPDLQKNTTLEDITKRAKIQNLDEEEKVKYLDSLKTYKFEEDPEYESYRVSSQVGMKKRSDGANLRIPIIEPKPVLEQIVSPLKQKLYKLNCQQNNSPFFVQNKLIKDDDKLYKLKLSAKELLSLEPSVYLQSYRIKSTPKKATQFLRLFVGKAFDAKMDVKKAITQCHFQKKKLAGEVAELLTKGLKDAEKLGINSDNLYLDQIWVGKDGFFGKAVDIKGRGRAGIITHKWVHIKAILRDRSVTVERLKHEKDLKQEKKKPWIQLEDKSIGGFPGGVYKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNF6</accession>
    <name>A0A1E5RNF6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g681</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNF6"/>
    <dbReference type="FunCoup" id="A0A1E5RNF6">
        <property type="interactions" value="598"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNF6"/>
    <dbReference type="InParanoid" id="A0A1E5RNF6"/>
    <dbReference type="OrthoDB" id="420076at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004741">
        <property type="term" value="F:[pyruvate dehydrogenase (acetyl-transferring)]-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF792">
        <property type="entry name" value="GM14286P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="7" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="6">
        <location>
            <begin position="149"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="534"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="534"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="556"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="567" mass="63005" checksum="B878A224E013F988" modified="2017-01-18" version="1">MLGRKASRCVAVLNKTAARSSLGLKTAKRMLSSSNWKLLSSKSVNFNSVKTAKFTAGINGAVGLLCVGVGGYSLYNKIEQQGNWFSEWSNSNIIKLDSFQKNFSSTSKAQEKDSQSQSINILAEEAIERKLHSNEYSYSVDREKSEIQRFDVSQLPSNDPIEDSHVEQILTNNGNDVWFYGVFDGHGGPYTSSKLSTDVIPYIARYLAPIFNKPEYSQQDVNNSFQKAFVELDNDIVLNGFKDLFDSPSRETLLEKTLPAISGSCCLLTMYDTKKSMLTISITGDSRAILVSKDHNSDGHGKSTNNWTVQALTKDQTAEVPEEIERIRKEHPEDESRTCIFNGRILGSLQPSRAFGDYRYKIDNVDGKKFSELPPEVKMFLRRSPRYLKTPPYVTAAPVVTSHKIDPSKDKFMVIASDGLYEFLNNDEIASLVVDWYSRQTSDNKDKFGNNGTIVVKDLDSLNPGLKHDSRFKTGLPSINKDENCSTHIIRNALSCGGQKEYVDTFVSIPSPMSRKYRDDLTVTVVFFGDSKDPITTTSSPSPSSFAAGDGVTLNEKASTPVSKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNH7</accession>
    <name>A0A1E5RNH7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mitochondrial inner membrane protease subunit</fullName>
            <ecNumber evidence="7">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g923</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase S26 family. IMP1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNH7"/>
    <dbReference type="FunCoup" id="A0A1E5RNH7">
        <property type="interactions" value="395"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNH7"/>
    <dbReference type="InParanoid" id="A0A1E5RNH7"/>
    <dbReference type="OrthoDB" id="308440at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042720">
        <property type="term" value="C:mitochondrial inner membrane peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.109.10">
        <property type="entry name" value="Umud Fragment, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052064">
        <property type="entry name" value="Mito_IMP1_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000223">
        <property type="entry name" value="Pept_S26A_signal_pept_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019757">
        <property type="entry name" value="Pept_S26A_signal_pept_1_Lys-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02227">
        <property type="entry name" value="sigpep_I_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12383:SF16">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12383">
        <property type="entry name" value="PROTEASE FAMILY S26 MITOCHONDRIAL INNER MEMBRANE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00727">
        <property type="entry name" value="LEADERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00760">
        <property type="entry name" value="SPASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2 7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 9" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S26" evidence="8">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ88447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="186" mass="21193" checksum="D11200D6895BC258" modified="2017-01-18" version="1">MLPTLNSTNDYVHVSKKYNNGKNIVMGDCVVAMKPSDPHQRVCKRVSGMPGDIILIDPSIGSKLNYVSEKMASMKSKKDSFEEIGTLLQETLSEYEPLLRRNSDMSNDDIDDEELRDNKRRLESFDEFVQVPKGHVWLTGDNLSYSVDSRTYNFVPMGLIKGKIVAANDFNKPFLQSFRQIDNSYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNI6</accession>
    <name>A0A1E5RNI6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">AWRI3579_g949</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 23">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the DEAD box helicase family. DECD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNI6"/>
    <dbReference type="FunCoup" id="A0A1E5RNI6">
        <property type="interactions" value="1292"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNI6"/>
    <dbReference type="InParanoid" id="A0A1E5RNI6"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17950">
        <property type="entry name" value="DEADc_DDX39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000809">
        <property type="entry name" value="ATP-dependent RNA helicase SUB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000111">
        <property type="entry name" value="DEAD-box ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 21" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="20">
        <location>
            <begin position="59"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="90"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="293"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="23"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="16">
        <location>
            <begin position="59"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="29"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OEJ88446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="444" mass="50116" checksum="35A495DC89ACC504" modified="2017-01-18" version="1">MSHEAEDDLLEYSDHEEQFQVPDSTQGVDAAGDSTAATTADSTGADGDKKGSYVGVLQTGFKDFLLKPELSRAIVDCGFEHPSEVQQSTIPQSIHGTDVLCQAKSGLGKTAVFVLSTLQQIDAVPGEVAVVVICHTRELAFQIRNEYLRFSKYMPDVKTSVFYGGTKYQDDIDKLKNKETCPNIIVATPGRCKALVRDKHANLSHVKMFVIDECDKVLEKADMRQDVQDIFRATPREKQVMMFTATLNDEMKPICRRFLQNPLEIFVDDEAKLTLHGLQQFYFKINENEKNKTLAKLLDDLEFNQVIIFVNSSKRANELTKLLQLSNFPAITVHGMMKQEERIERYKAFKDFDKRICVSTDVFGRGIDIERINLAINYDLPKEADQYLHRVGRAGRFGTKGLAVSFVSSAEDEEILEKIQSRFDVKIAPFPEEEGIDPSTYLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNS6</accession>
    <name>A0A1E5RNS6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g972</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNS6"/>
    <dbReference type="FunCoup" id="A0A1E5RNS6">
        <property type="interactions" value="576"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNS6"/>
    <dbReference type="InParanoid" id="A0A1E5RNS6"/>
    <dbReference type="OrthoDB" id="5348404at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005178">
        <property type="entry name" value="Ostalpha/TMEM184C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23423">
        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03619">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01417">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ88520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="354" mass="40591" checksum="35193472E5E330C8" modified="2017-01-18" version="1">MELGWGKPLIRHPLLGRFLPPVDIADPKDFLSIKRGVLQYVWFKPFYIAGKLLVDLTPRDEITKQPPVWCLRLETVLLIGYNISASLSLYDLALFWRCLYTELAKYKPWPKFLCVKLIIFASYWQGVLLLILNHYGIFTENENGQDMGFIYQNSLLCIEMIGFSLGHLIAFSSEPYSFRKLPTCSRIKFFYALKDCCGVGDLISDFKTTFFGTQYNYRNFDSAEAVLTSNAHVRTRNARLNEGLRFSNNGTHQYWLQQPVNYGSIDAAGSDAASVLATMSNDNAAPIEQPSSPRRTLAECDHIDSEPWTELLDKVSTRYIPEDPNYPVVWDIRGHRYKPEMQKLKRASEPVNDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNT6</accession>
    <name>A0A1E5RNT6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Cysteine proteinase 1, mitochondrial</fullName>
            <ecNumber evidence="2 9">3.4.22.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g578</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Has aminopeptidase activity, shortening substrate peptides sequentially by 1 amino acid. Has bleomycin hydrolase activity, which can protect the cell from the toxic effects of bleomycin. Has homocysteine-thiolactonase activity, protecting the cell against homocysteine toxicity.</text>
    </comment>
    <comment type="function">
        <text evidence="7">The normal physiological role of the enzyme is unknown, but it is not essential for the viability of yeast cells. Has aminopeptidase activity, shortening substrate peptides sequentially by 1 amino acid. Has bleomycin hydrolase activity, which can protect the cell from the toxic effects of bleomycin. Has homocysteine-thiolactonase activity, protecting the cell against homocysteine toxicity. Acts as a repressor in the GAL4 regulatory system, but this does not require either the peptidase or nucleic acid-binding activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred.</text>
            <dbReference type="EC" id="3.4.22.40"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homohexamer. Binds to nucleic acids. Binds single-stranded DNA and RNA with higher affinity than double-stranded DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase C1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.40" evidence="2 9"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNT6"/>
    <dbReference type="FunCoup" id="A0A1E5RNT6">
        <property type="interactions" value="707"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RNT6"/>
    <dbReference type="OrthoDB" id="2666448at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070005">
        <property type="term" value="F:cysteine-type aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043418">
        <property type="term" value="P:homocysteine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00585">
        <property type="entry name" value="Peptidase_C1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004134">
        <property type="entry name" value="Peptidase_C1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10363">
        <property type="entry name" value="BLEOMYCIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10363:SF2">
        <property type="entry name" value="BLEOMYCIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03051">
        <property type="entry name" value="Peptidase_C1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005700">
        <property type="entry name" value="PepC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0788">Thiol protease</keyword>
    <feature type="active site" evidence="10">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="448"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR005700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005700-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ88537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="530" mass="61119" checksum="EB68E34E4F887631" modified="2017-01-18" version="1">MTSLQKVFVFKASVFKHCLPVAFPVLHSLLHVAVNRVTFKRPTHRMVNPIFNLKQINQLKQEPFIEINKQEIEKNTTTMSLNSKELENWESEFKQDKTHNLASTVLKNSNADEALLDKTAMLSIDKQTYNIGIPKDELVTPVANQKSSGRCWLFAATNCLRLQIAKDLNIKENFEISQAYLFFYDKLEKCCYFLDQIIDTADQDVDSRLVQYMLQIPINDGGQYSMFLNIVNKYGLMPKDLYNDLPYSTTASAKWNNLLVMKLREFAQILREHINVKKASSKDVQILKKSMQKEIFRLMSLFMDPPSVKPDESFTWEYSDKDKILRTVTCTPKEFASKICKVDSKKCVSLVNDPRNEYNKLIKVDRLGNVTGGDPVLYLNVDNKTLTSLAVKRLKAKKPIFFGAHTPMFMDKKSGVMSSKIYNYDAIDYNFKQDKKNRIQHGNSLMTHAMTITNAYIDSNTETPIRYKVENSWGKESGNDGYYMMDQEYFEEYCFQIVCDLDELPKELQAKLDDSKPILLPLYDPMGALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNU2</accession>
    <name>A0A1E5RNU2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mannosyltransferase</fullName>
            <ecNumber evidence="12">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g901</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the third mannose to Man2-GlcN-acyl-PI during GPI precursor assembly.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 22 family. PIGB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="12"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNU2"/>
    <dbReference type="FunCoup" id="A0A1E5RNU2">
        <property type="interactions" value="868"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNU2"/>
    <dbReference type="InParanoid" id="A0A1E5RNU2"/>
    <dbReference type="OrthoDB" id="416834at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF4">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 12 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="275"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="358"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="385"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="415"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ88552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="595" mass="69932" checksum="76F2E98C7AAC1A7A" modified="2017-01-18" version="1">MVNQGIVGASSFKKLLKPLIVFRVLSALLINSYFQADEFWQCLEPAHYKAFGYGELTWEWKEGLRSYAFPFLFQMVYNVCKLISLVCTWALLYCENFCQNIMMHSTSLKKYDFFWDVLDYVHYLRLNFPADIEYYSVIYLPKVAMALIAAVGEYYTIGLIYKVYLLTFAKKYPKDANAIIKIALVLTLSNFFNCFFITRTFINSFEMSVTAIALYYWPWSDSLEELRSPFFRLSLFLSFFMCLQRPTNGLTWFCFGLYTVYKLLSKKKFAEIKTLMFNITTMGALAVVLNCCIDFYFYHELCFPVLKFIKFNVTKSLSVFYGSAPWHFHIAQSFPIINGYSLPLVIYGLVTFFDTPNAHIFSIMKVTVLFTTTVFSLIKHKEFRFLFPMQPLFMVFSTFSLFAISKKYALKLESFAYLITPIASVIAALVIVRFQETGVIQVIDLLHNLPYPIESAGFIMPCHSTPWQSHLHRPNINDLWAITCEPPLHLLEEADSDSLIKSYMDESDYLYDNIHKFIYLNFPPVFNKKLRSPEKTFLHEWPEYLIIFEHMDSEFMKKKLEDSAYFEYARFFNSWSHWDSRRSGDVIVYHKPSWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNU4</accession>
    <name>A0A1E5RNU4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">L-2-hydroxyglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="7">1.1.99.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g903</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(S)-2-hydroxyglutarate + A = 2-oxoglutarate + AH2</text>
            <dbReference type="Rhea" id="RHEA:21252"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:16782"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="EC" id="1.1.99.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the L2HGDH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.2" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNU4"/>
    <dbReference type="STRING" id="56408.A0A1E5RNU4"/>
    <dbReference type="InParanoid" id="A0A1E5RNU4"/>
    <dbReference type="OrthoDB" id="498204at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047545">
        <property type="term" value="F:(S)-2-hydroxyglutarate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43104">
        <property type="entry name" value="L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43104:SF4">
        <property type="entry name" value="L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="9">
        <location>
            <begin position="27"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ88554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="396" mass="44015" checksum="6BD058E266337A28" modified="2017-01-18" version="1">MFSKIKAPSIQFATTHFKRFSSDFTHCVIGAGVVGLAVAAELSKIPEANVIVLEKNYKTGMETSSRNSEVIHAGLYYPSGSLKSKLCIEGKNIIYNELKPRLTGVDWIKCGKIVVAQNDYEDSEMEGLYYKCKNELDIPAELIPSTKLKYIEPAIEALRGAMLSPSTGIIDSHSLMSYYESCLKENGADIILNTEVKNIEYSQNSAYSMVCEDAESKEEVVLTVDNIINCAGLHSHKVANMLLPKDRQLQQYYGKGNYFQLSTSAVPKVKHLIYPVPPKHGKSLGTHLTLDMSGNIRFGPDFEYVESPTDYSVSTKNLMAAYEAIIKYYPHITPDQLIPAYSGIRPKLLGPGEKGFSDFYIQEEDGFKGFINLMGIESPGLTSSPAIARYIRKTFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP22</accession>
    <name>A0A1E5RP22_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycine zipper 2TM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g611</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP22"/>
    <dbReference type="FunCoup" id="A0A1E5RP22">
        <property type="interactions" value="229"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP22"/>
    <dbReference type="InParanoid" id="A0A1E5RP22"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019867">
        <property type="term" value="C:outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008816">
        <property type="entry name" value="Gly_zipper_2TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05433">
        <property type="entry name" value="Rick_17kDa_Anti"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycine zipper 2TM" evidence="2">
        <location>
            <begin position="42"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="219" mass="21060" checksum="7F158F6A61DF5AB8" modified="2017-01-18" version="1">MSANEFYQGADKTQNQAYQGQNEQAYQAQAQQNGETNDKGLMSTVLGAAAGGYAGNQGSSNHSTLGGIGGALGGAFLANKVSDAFENHGNNNNNNNNNNGNYGRPQQQQGGEGFLGKIFGGGNNDHNNEGRHQGHGGPGGFGGPGGPGGPGGFGGPGGNQGGFGGPGGNQGGFGGPGGPGGPGGFGGPGGNQGGYGGNQGGYGGNQGGYGGQGNQGGRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVA5</accession>
    <name>A0A1E5RVA5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">60S ribosomal protein L30</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g526</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVA5"/>
    <dbReference type="FunCoup" id="A0A1E5RVA5">
        <property type="interactions" value="1268"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVA5"/>
    <dbReference type="InParanoid" id="A0A1E5RVA5"/>
    <dbReference type="OrthoDB" id="1928736at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000001">
        <property type="entry name" value="60S ribosomal protein L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039109">
        <property type="entry name" value="Ribosomal_eL30-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022991">
        <property type="entry name" value="Ribosomal_eL30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002172">
        <property type="entry name" value="PRK01018.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11449">
        <property type="entry name" value="RIBOSOMAL PROTEIN L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00709">
        <property type="entry name" value="RIBOSOMAL_L30E_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00993">
        <property type="entry name" value="RIBOSOMAL_L30E_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="4">
        <location>
            <begin position="26"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ90861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="122" mass="13130" checksum="2D462174A339286E" modified="2017-01-18" version="1">MHGFVSTRSSLGTTVILLAPAKNTDTVNQKLALVIKSGKYTLGYKSTVKSLRQGSAKLIIIASNTPVLRKSELEYYAMLSKTKVYYFQGGNNELGTAVGKLFRVGVLTILEAGDSDILTTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVP7</accession>
    <name>A0A1E5RVP7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 13">Tryptophan synthase</fullName>
            <ecNumber evidence="5 13">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g542</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the TrpB family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the TrpA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="5 13"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVP7">
        <property type="interactions" value="495"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVP7"/>
    <dbReference type="InParanoid" id="A0A1E5RVP7"/>
    <dbReference type="OrthoDB" id="10050244at2759"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06446">
        <property type="entry name" value="Trp-synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04724">
        <property type="entry name" value="Tryptophan_synthase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000151">
        <property type="entry name" value="Tryptophan synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000001">
        <property type="entry name" value="Tryptophan synthase beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000004">
        <property type="entry name" value="Tryptophan synthase beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00131">
        <property type="entry name" value="Trp_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00133">
        <property type="entry name" value="Trp_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006653">
        <property type="entry name" value="Trp_synth_b_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006654">
        <property type="entry name" value="Trp_synth_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018204">
        <property type="entry name" value="Trp_synthase_alpha_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002028">
        <property type="entry name" value="Trp_synthase_suA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00262">
        <property type="entry name" value="trpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00263">
        <property type="entry name" value="trpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077:SF3">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00290">
        <property type="entry name" value="Trp_syntA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00167">
        <property type="entry name" value="TRP_SYNTHASE_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00168">
        <property type="entry name" value="TRP_SYNTHASE_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 13" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="11 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 13" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="14">
        <location>
            <begin position="359"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="15">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ90906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="716" mass="77276" checksum="5B64AF868399FF45" modified="2017-01-18" version="1">MSALLKKAFTQARAENRNALVTFMTAGFPTSDSTVPIMRAFQDSGVDVIELGMPFSDPIADGPTIQTANTKALDNGVLTGDVIDYVKEARAQGVTIPVLLMGYYNPILSYGEEKFIKEAAEAGVNGFLIVDLPPEEAIKYRNFCNKYGLSLVPLVAPSTTDKRLEVLAGQLADSFIYVVSRMGTTGVQSQSVATSSLDELCARVRKYSKDIPIAVGFGVSTRDHFTAVGKSADGVVIGSKIINLVADCKTKEEAYTAVKQYCEEILAGYKHKVLTIEEFNAAKEQSLKGVAPAAGSSESKXDIEFNEKHVTPQNFGDFGGQYVPEALHACLRELEDAFDAANADPEFWKEFRGLYDYIGRPSSLHKAERLSEHCGGATIWLKREDLNHTGSHKINNALAQVLIAKRLGKKKIIAETGAGQHGVATATACAKFGMECTVFMGAEDCRRQALNVFRMKILGANVVPVQNGTRTLRDATSEAFRFWVSNLESTHYVVGSAIGPHPYPTLVRTFQSVIGQETKEQFKKLNDGKLPDAVVACVGGGSNSTGMFSPFEKDLSVKMLGVEAGGDGLETGIHSATLTAGRPGVFHGVRTFVLQDTDGQIHDTHSVSAGLDYPGVGPELAMWKATGRAEFIAATDAEALEGFKYLSQLEGIIPALESSHAIFGGMQLAKKMNKNQNIVINVSGRGDKDVQTVAEVLPKLGPKINWDLRFEEDPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVR0</accession>
    <name>A0A1E5RVR0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ribonucleases P/MRP protein subunit POP8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g485</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVR0"/>
    <dbReference type="FunCoup" id="A0A1E5RVR0">
        <property type="interactions" value="114"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVR0"/>
    <dbReference type="InParanoid" id="A0A1E5RVR0"/>
    <dbReference type="OrthoDB" id="3973016at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000171">
        <property type="term" value="F:ribonuclease MRP activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034965">
        <property type="term" value="P:intronic box C/D snoRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000294">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, RNase MRP-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020347">
        <property type="entry name" value="Pop8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049128">
        <property type="entry name" value="Pop8-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28173">
        <property type="entry name" value="RIBONUCLEASES P/MRP PROTEIN SUBUNIT POP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28173:SF1">
        <property type="entry name" value="RIBONUCLEASES P_MRP PROTEIN SUBUNIT POP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20976">
        <property type="entry name" value="Pop8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribonucleases P/MRP subunit Pop8-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="130" mass="15218" checksum="C006A1841FF2DB85" modified="2017-01-18" version="1">MSKEWCYYELKITSQSPSLDKETLIDTITLKRFISMALQKYHGLFGQGIEYSIMNHHNQTAFIKVSQIDKSCFASAVTLYVSTEDLYDHPLVALLEQETSDIKTLSVRKDDQLWWKRECEEEEEEEEDAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVY9</accession>
    <name>A0A1E5RVY9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Golgi apparatus membrane protein TVP23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g433</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Golgi membrane protein involved in vesicular trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Golgi apparatus membrane</location>
            <topology evidence="2 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the TVP23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVY9"/>
    <dbReference type="FunCoup" id="A0A1E5RVY9">
        <property type="interactions" value="401"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVY9"/>
    <dbReference type="InParanoid" id="A0A1E5RVY9"/>
    <dbReference type="OrthoDB" id="2151161at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008564">
        <property type="entry name" value="TVP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019:SF7">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05832">
        <property type="entry name" value="DUF846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="31"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ91026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="188" mass="22154" checksum="99446AA13C980C49" modified="2017-01-18" version="1">MDHVKNFYETILKSSHPLTSSLHLGFKAIPLVIYFLGSLMFHSFTTIAIFIILTISVDFYYTKNISGRKLVQLRWWYDENLTPPFKIESYKQYDQETVINPIDDKIFWWGLYGSFLVWCFSSIVCLMKFKLIYLVLVAFAIGLNYYNCRGFRTCYYFKPNGQASLQDSFNDIWGKVKNLNSLTTLFQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RW13</accession>
    <name>A0A1E5RW13_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cleavage and polyadenylation specificity factor subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cleavage and polyadenylation specificity factor 100 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g347</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF2/YSH1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RW13">
        <property type="interactions" value="859"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RW13"/>
    <dbReference type="InParanoid" id="A0A1E5RW13"/>
    <dbReference type="OrthoDB" id="64353at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027075">
        <property type="entry name" value="CPSF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025069">
        <property type="entry name" value="Cpsf2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45922">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45922:SF1">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13299">
        <property type="entry name" value="CPSF100_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16661">
        <property type="entry name" value="Lactamase_B_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="4">
        <location>
            <begin position="91"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Cleavage and polyadenylation specificity factor 2 C-terminal" evidence="3">
        <location>
            <begin position="811"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="55"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="523"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="654"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="530"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="654"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="667"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="706"/>
            <end position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ90985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="954" mass="109644" checksum="99EA37EAC92648FB" modified="2017-01-18" version="1">MTFGFTSLCDYSASALNEGVDTQLRETAKLVFNGSIITFNDFTILIDPGWISQKNNNDDDDDNDDNDDNDHHSKNTNDSIVKSTEPEKNVWQTEDLYQSLVDFWTPIVEKVDLILISQSSVDCLGSYAFIFSHFQSHLYNNNVKVYSTLPVSNLGRVSTIDLYCSLQMTGPFCSSKMEVEDIENAFDYIITLKYSQMINVQEYKSDKLFFTAYSSGHSIGGCSYCIENSQEKIIYSPKWSHTKDVLVKGTQMLSQENNLSPIKSLMKPSCFITSMKYTILSNPLPLRRKIGDFKKYLKETLKGNNCILIPTSIGMDFLNIFVLLHDYFNDLKTRKSGNQRGSQKQQQQQQQFNHQPVYLVSYSKGRSLTYAKSMLEWLSNDTVKQWQARDNKTPFDLDVKVLSPLDFQXRKNEIIMKGGIFFVSDDEVLFNQVLTSFIANNQMLKNCHVLMTTAFXNSPLVQDMLKTSNDDIEYNKVVDLKEYQVEXLSDAEYVSYXEVIKERNSKRQVLKYEYETIQESNRKQNLNSLLEEDSEEDEDDDEDADEDEDDEENSEENGERRKXNPNIASSEKEKKNSEHLNGTTKDTYGEDGELKIPMDIIIKENAPIKQKMFPYRFSKNVIKDDYGLLVDFSQFVPIDETEDDYVLDLKRTREESRITSRSGKNDNDDDAEDGQDDDDDDDDDDEDDDDYDPTKTISKPNAYKKRATEKERESQNFDDISYLQPLDSKPTKAQLVEQQYKLACKIEYIDMTSYIDQRSMDIILPSFQPGRLLLIDSFPTEKTYPNTETVSITDNKEHIFSSSIKVLDIAISPELEKQIHWQSVLNGKYKITNVSGRLVKLLDKSAEQDKNERGHLSETKLKLELKPLNEKKIAFSDQEHDYNNLHLGDIKLNQLKELLVQMNHQAQFKGDGTLVVDNVVFIKKISDSVTVIDGKPSKLFYTVKKVVASTLATI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RW36</accession>
    <name>A0A1E5RW36_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g369</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RW36"/>
    <dbReference type="InParanoid" id="A0A1E5RW36"/>
    <dbReference type="OrthoDB" id="5555533at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070096">
        <property type="term" value="P:mitochondrial outer membrane translocase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037652">
        <property type="entry name" value="Mim2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19117">
        <property type="entry name" value="Mim2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="116"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="39"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ90943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="180" mass="20492" checksum="E00F9D5F7AEEBBCF" modified="2017-01-18" version="1">MSSRITGLQSNPFELLNDPISSSDEELSDIHSHASLXDNEADDXDDDDDDEVNEATENILDSKANSDPFAAVDEDSENYNDVQDELPSNSDEFVVVETETETETEIELEVEYTEYDDELLDEYENLEQERLAKINQEWQESVAQLKQAVNWIVLPLIGKLVGRKCARLVWNFACRKLYGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RYR4</accession>
    <name>A0A1E5RYR4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">NADH-cytochrome b5 reductase</fullName>
            <ecNumber evidence="12">1.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g271</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2 Fe(III)-[cytochrome b5] + NADH = 2 Fe(II)-[cytochrome b5] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46680"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11 12">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.6.2.2" evidence="12"/>
    <dbReference type="EMBL" id="LPNM01000002">
        <property type="protein sequence ID" value="OEJ92140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RYR4"/>
    <dbReference type="FunCoup" id="A0A1E5RYR4">
        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RYR4"/>
    <dbReference type="InParanoid" id="A0A1E5RYR4"/>
    <dbReference type="OrthoDB" id="432685at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090524">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NADH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06183">
        <property type="entry name" value="cyt_b5_reduct_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000009">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001834">
        <property type="entry name" value="CBR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370:SF143">
        <property type="entry name" value="PLASMA MEMBRANE-ASSOCIATED COENZYME Q6 REDUCTASE PGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00406">
        <property type="entry name" value="CYTB5RDTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 12" id="KW-0274">FAD</keyword>
    <keyword evidence="4 11 12" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0520">NAD</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="13">
        <location>
            <begin position="84"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601834-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ92140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="326" mass="36594" checksum="A3133FC00C1F81A8" modified="2017-01-18" version="1">MSREEKVAALNKITEEDIEAEPVNILDEPLHGIYIPSGLLVAGLSILYKLSGETKILYVIPIYLIFITVKVLQAKRNSKSVYSDKWNALELVDKTLISKNTAIYKFALNSKLDSLNFPVGHHLAVKIPIEGKEDQVRYYTPISPQHQEGTFDILVKSYGDGEVSKWFATQSYTGMKVEFKGPVGRFGYKPCKEIAMIAGGSGITPMLQVLSKVIVDSSDSSNLTLIYANETENDILLKDELDEIAEKSPNFKVHYVLNKPDEKWDGEKGLVTKEIMEKYLPKCSLDSRLLICGKPEMKKMLLDYAEQLGWPKGDMKSAADDQVFVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZI1</accession>
    <name>A0A1E5RZI1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein bL27m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">54S ribosomal protein L2, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g148</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bL27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZI1">
        <property type="interactions" value="294"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZI1"/>
    <dbReference type="InParanoid" id="A0A1E5RZI1"/>
    <dbReference type="OrthoDB" id="1867012at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000042">
        <property type="entry name" value="50S ribosomal protein L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001684">
        <property type="entry name" value="Ribosomal_bL27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018261">
        <property type="entry name" value="Ribosomal_bL27_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041244">
        <property type="entry name" value="Ribosomal_bL27m_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00062">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15893:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL27M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15893">
        <property type="entry name" value="RIBOSOMAL PROTEIN L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01016">
        <property type="entry name" value="Ribosomal_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18471">
        <property type="entry name" value="Ribosomal_L27_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00063">
        <property type="entry name" value="RIBOSOMALL27"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110324">
        <property type="entry name" value="Ribosomal L27 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00831">
        <property type="entry name" value="RIBOSOMAL_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 11" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Large ribosomal subunit protein bL27m C-terminal" evidence="10">
        <location>
            <begin position="137"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ92382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="376" mass="43119" checksum="5C25F6EAC40F0358" modified="2017-01-18" version="1">MFKKGGICMQWLGRANXNTLLFAFKQVRTATKKASGSKTSMKDSAGRRLGPKKGNGAYVEPGQIIYRQRGTKFYPGENCSIGRDHTIFAKEPGWVQYYLDPFHPKRNLIGISLFREKKLPTPHFAPRIRRLGRRVIEDEKAAEKEASSLNRKQFLAKDELVAQHEGRENKRLELLKSFEEFLTRNFASNETFSGNLEFCGKALLLIRSNLRQGFSRHESLFNVLAHFKHLKKIGSHVENNGLPDVQVVESCLVEIQKYIDFNHDFEIVEYMTSEKKLAKQQEIKAQVAELLPLNTKDKVXQAKEIFNKPELKAYFTKSEYVKFKKTVIKPVLPESVEGTVVSKTTKGATATRRYNYDTGRISTVYRSKHAFLEPLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZN6</accession>
    <name>A0A1E5RZN6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g93</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZN6">
        <property type="interactions" value="676"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZN6"/>
    <dbReference type="InParanoid" id="A0A1E5RZN6"/>
    <dbReference type="OrthoDB" id="413460at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18000">
        <property type="entry name" value="DEXHc_ERCC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000039">
        <property type="entry name" value="DNA repair protein Rhp26/Rad26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050496">
        <property type="entry name" value="SNF2_RAD54_helicase_repair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058951">
        <property type="entry name" value="WHD_Rad26_CSB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629:SF7">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
        <property type="entry name" value="SNF2/RAD54 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25875">
        <property type="entry name" value="WHD_Rad26_CSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="321"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="666"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="107"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="989"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1094"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="37"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="123"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="151"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="172"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="187"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="996"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1099"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1109"/>
            <end position="1119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ92411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1119" mass="127074" checksum="4D9CABE818471C1D" modified="2017-01-18" version="1">MPXDDSTNREFDNVIEVVPQSTLENQIENDAXSLMNKKALEQEKDRLERTLNKISQLKKRRKHLENRIKNATRISAKKKLKDDLAKMEVEQFTPVLQDVKDINERIKSLEKQMRQPEQAQNDTSKRPGESQKEYLIRTGQITAFGNKSGFEMDDSDQDNDVPSETEHKNENISKGNLKENSLGASRTLHDTHENHDSDESYMSVEDPDESSDYEPENISNEGADESDSLVYETESSKQKAPLIKSSDARDDGDELHYQKRLHQWSALRSKGRAKDFYPSDPEWFKPHPNYKDAHLNDGFKIPGEIFSKLFNYQKTAVQWLWELHQQNCGGVLGDEMGLGKTIQIIAFLASLHHSKKLHKPILIVCPATLMKQWVSELHFWWPPFRSIILHSVGSGFEKGKNKMSEQELEELMVTSENFSYQDYTDSSNINNLFSTSKAISNLVDKVVAQGHIVITTYVGLKIYSKVLLDVDWGYVILDEGHKIRNPNADISLTCKKLRSKHRLILSGTPIQNNLNELWSIFDFVYPGKLGTLPVFQQQFAVPISMGGYANATNLQVQTGYKCAVVLRNLISPYFLRRVKADVAKDLPEKSELVLFCKLTKYQRNKYLEFLNSEELTKIKKGKMQVLYGIDVLRKICNHPDLLVGKKYKSERPKDYGNPLRSGKMQVVKQLLLKWKQEGHKTLLFTQTKQMLDIIQDFISVADKEMAGNLTWLRMDGSSNIATRQKLVDEFNNEPVDIFLLTTRVGGLGVNLVGASRIIIFDPDWNPSTDLQARERAWRIGQTKEVTIYRLMISGSIEEKIYHRQIFKQFLTNKILKDPNQKRFFKMNELHDLFTLGADRGQNEEFNRSIKEVTQREEXNVQDDFESVAGVSKLEGFYNVKEEEERKKNEDERMMEGLFGVEGVIDHDNIVGSHTRPSEDIISREASKAAERAAAELKKSRQVTKSFKVGVPTWTGKFGVAGKVAKPGLGKIKGKNSTGSLAILSRMKSLKHGPGNAKSSASSPLASSSEVTIRKDVDPSTELTGASAATVSNEIREIKDYLSSQPNNFAPSASVINALGIDVKHRHDIVRIRALLRKIATFDVNKKGWTLSQEFRDQNSNESQFXDTNSSSGFSSSEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZX4</accession>
    <name>A0A1E5RZX4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g243</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZX4"/>
    <dbReference type="InParanoid" id="A0A1E5RZX4"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ92434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="129" mass="14659" checksum="82E7FEC848EF4183" modified="2017-01-18" version="1">MSTYSQLHWDQHQLSPTTSFPFHLTIHSALSLAVKGLYSSPTTPLTSNISFGISGMFLYQQPSIGQPKNITTWSTRYHTHSFDKHPNYTATVPRVSQFPGDVNHSHKLPFHTLHYTSNTSYLSLLRLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S012</accession>
    <name>A0A1E5S012_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA polymerase delta subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g184</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S012"/>
    <dbReference type="InParanoid" id="A0A1E5S012"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="414"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="201"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="290"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="454" mass="50784" checksum="9C3BCF63895E9FE1" modified="2017-01-18" version="1">MTTLEQYVHESLLNNVSLEKHTASEVSVVDVAQKFDWSIAQSRKAMYEYYSLQGNKMGDKLTFNMMCVYAEKDEERNELREGQHSHIKHFKLIKSPTDQQFVLEQVAPENIIDMFVYSIGLKHHGTGEGEDNLCVASLKPVQKVFVTHGKKLDLDENALEQDKTNVNDKIKSTTNFTKRSATEPAKXLEKRKXTSVADIRASSVATTPTATANNPSGTDSSSTSNNNSSSTSNNNSSSTSXTNTSSKSQKKASSFMGLRSMDFLAKRKEQDELKEQQRLQELREKRGVNTTTSTKTKPTASHNTNNAAKKPKIDLEKQKKLKELESMFSDSDDNEXETMPAENTKTQTDLAEVENLFDTTNDDSEMAELLTKGKQQQKEEEPPVKRSSPLKKTPDTYVDEEGYIVSTNLNANKRPAKRSASTLPLVSSAKTAGFETANKKRKQGNLMSFFSKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S098</accession>
    <name>A0A1E5S098_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">S-formylglutathione hydrolase</fullName>
            <ecNumber evidence="2 7">3.1.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g52</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Serine hydrolase involved in the detoxification of formaldehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>S-formylglutathione + H2O = formate + glutathione + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14961"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="EC" id="3.1.2.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the esterase D family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.12" evidence="2 7"/>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5S098"/>
    <dbReference type="FunCoup" id="A0A1E5S098">
        <property type="interactions" value="663"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S098"/>
    <dbReference type="InParanoid" id="A0A1E5S098"/>
    <dbReference type="OrthoDB" id="420518at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018738">
        <property type="term" value="F:S-formylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046294">
        <property type="term" value="P:formaldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000002">
        <property type="entry name" value="S-formylglutathione hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000801">
        <property type="entry name" value="Esterase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014186">
        <property type="entry name" value="S-formylglutathione_hydrol"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02821">
        <property type="entry name" value="fghA_ester_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10061">
        <property type="entry name" value="S-FORMYLGLUTATHIONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10061:SF0">
        <property type="entry name" value="S-FORMYLGLUTATHIONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00756">
        <property type="entry name" value="Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0719">Serine esterase</keyword>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR614186-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ92438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="299" mass="33066" checksum="2AECB59F3FB890FA" modified="2017-01-18" version="1">MSLNINSKIASCGGKLLKCTHDSVATKTAMNVNVYVPKQFYTAAKTSQKIPTLYFLSGLTCTPDNASEKAFWQLQADIYGFAVVFPDTSPRGDNVPGDPENSWDFGKGAGFYVNATTGKYKENYNMYDYVHSELPQLLQKNKDIFEDKIDFLENVSIAGHSMGGFGALSGFLKNLNKYKSCSAFAPIVNPSVVPWGTKAFGGYLGETSKSKWDEYDPCTLLKNTENPDNKYKIMIHVGTADPFLEKQLKPQNIVAAAKGTTWENAIDLNIVDGFDHSYYFISSFVPAHAEFHAKHLGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R001</accession>
    <name>A0A1E5R001_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein GIS4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4647</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000017">
        <property type="protein sequence ID" value="OEJ80230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R001"/>
    <dbReference type="FunCoup" id="A0A1E5R001">
        <property type="interactions" value="147"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R001"/>
    <dbReference type="OrthoDB" id="3972606at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="547"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="684"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="731"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="213"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="684"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="735"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="756"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="769"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="790"/>
            <end position="799"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="816" mass="92592" checksum="14A94A55C7100C0C" modified="2017-01-18" version="1">MPHIITVKDYYENDYNGLWSWYLNNLRKGEFEELTGNILKYSLLKKFLKEFFYKNNDITNDNHENSDKKILLVSVPDKIYKNQTNYKINILENFLKDYFKLDDLTNIHIEKLTKKNCKIYNHETHYLIMDPLNNFKDKNLFKPNNVEFLKEDNEQTQSFNNSSIDTRTTDGNQLKVSVKPVSSSLTAISQLAHQTVQQLSSHDTESYNDNEDSVSMVSEMSSSSSSSSSASSPTSSSSLSSSGSSTTTSNDSQTQSELQIDFANKLKMNKNDESLQESCLTKKITKETQQIALNGTKNDSAQMDTSNTKDKPRTTSSNNRSSVHTQTTTNTHRQLLNNNGHLDNDVQMSIASFNYTTDDDFGAELQYVDTRDDHSYDAESQHQQGETSVFEDTDSEYIDDMGSSVLDEELDHEHGHNGCGRNGNDDDDYSMNSIFPSISIKDDFGEFRLVLQSILIENPEYDINNKQVDNLITNTAIRQSNMDPTNASIHDDWLLYDAKFDITNLQMLSLSDVLYLNKNCPKMLFYTMVDTSNPENLPQEEIKDIKTDSHHHHRNSPVPLLQQESETQDKDMENEEEEIQEFNADMVKKNINSFSTTNFLQDFENQSADGRSQYSDDRGSYSDISSEVKLYNANTNVTTTSRNPYVVQSFYGNDPKREDDSTIYDGISRQNTNARSFAESNMSFASGLSNNGESKNPEKTNSKHKNKKSGKSVGKDLTKVFTLGSLHLVERSKTLPPTTTQSNTEAAIAKQENTRYKRLLMRRKPTSRSKNNSDNNGHHGNNSKSAGSGTSNREQTFSKKGSKKNHRSHDNNCVIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R025</accession>
    <name>A0A1E5R025_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Low temperature requirement protein A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4659</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000017">
        <property type="protein sequence ID" value="OEJ80236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R025"/>
    <dbReference type="InParanoid" id="A0A1E5R025"/>
    <dbReference type="OrthoDB" id="191995at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010640">
        <property type="entry name" value="Low_temperature_requirement_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36840">
        <property type="entry name" value="BLL5714 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36840:SF1">
        <property type="entry name" value="BLL5714 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06772">
        <property type="entry name" value="LtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="271"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="299"/>
            <end position="318"/>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="338"/>
            <end position="355"/>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="388"/>
            <end position="410"/>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="431"/>
            <end position="464"/>
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        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="491"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="538"/>
            <end position="548"/>
        </location>
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        <location>
            <begin position="568"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        <source>
            <dbReference type="EMBL" id="OEJ80236.1"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="605" mass="68272" checksum="BC1FF3B72B38DA74" modified="2017-01-18" version="1">MSDLEANSSHSSVPSRQLGARNGESRHAGFSDKYGPVKCLWIEPPASAAENKLIVKPFAWNYFYNGILYRTKEERQPTLAELFFDLLYVGITANLANTCIEYSVGSSLAKYILLFLPCWHVWSDMRAFMDYYYNNDVTQNAYLLWIMFLLVTYANNAHTVTLNEKPLIGLVVACYMLARFSYATMLLIYNLFFIKEHRKQMMWYCGSLYISVIMAGFVLLPXHMYQRIIIVCCLYFWDALSYSITFSAWFKKLINTPYHTAMSIEHEIKRHNSFVTIAIGEFLYPTVALTPASGGLNERTARCTCILILAFCLAWFYFSGEGSRKAIHALRRHSLTGLCWIQFHFFLIVSLALAANSGGVLTETKYTTNTDSSGEESPMARSNYLQDVQIYFGSGLAVALVSMTVIAMLDKGLDEPIFWKLTPKARILPRFIMAAVTFGLSFAEINITLYLGIVSLLLLIQLIFEKVADVKSHAYKIKLNSVAQASLASSQSDEDSGLVHEQQGSINTDYEPDAASDYSVSSEKQRELDCHMPLPQPAHNNSNGQETDTXPXMKLQQQHSTKEEQSIAEAQQGSINTDYXPETDNETPQTHSKTKELDCSHTXTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0C0</accession>
    <name>A0A1E5R0C0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">V-type ATPase assembly factor PKR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4403</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0C0"/>
    <dbReference type="FunCoup" id="A0A1E5R0C0">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R0C0"/>
    <dbReference type="OrthoDB" id="9626941at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013945">
        <property type="entry name" value="Pkr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28251">
        <property type="entry name" value="V-TYPE ATPASE ASSEMBLY FACTOR PKR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28251:SF1">
        <property type="entry name" value="V-TYPE ATPASE ASSEMBLY FACTOR PKR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08636">
        <property type="entry name" value="Pkr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="109" mass="12290" checksum="D6D7C09AFD102416" modified="2017-01-18" version="1">MGNFVVDLWDSIFQPGTTPTLILATHLSFIALFSVLGWLIYVTKAQNIHFIMLLLIAIALWVTIIWFTSELQSVKLKSNEELFGEDMTTIPEKDTTPSRNGSSKDSKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R104</accession>
    <name>A0A1E5R104_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 15">Ribulose-phosphate 3-epimerase</fullName>
            <ecNumber evidence="8 15">5.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AWRI3579_g4431</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>D-ribulose 5-phosphate = D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13677"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="EC" id="5.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="17">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Carbohydrate degradation; pentose phosphate pathway; D-xylulose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 15">Belongs to the ribulose-phosphate 3-epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.1" evidence="8 15"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R104"/>
    <dbReference type="FunCoup" id="A0A1E5R104">
        <property type="interactions" value="459"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R104"/>
    <dbReference type="InParanoid" id="A0A1E5R104"/>
    <dbReference type="OrthoDB" id="1927044at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00411"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004750">
        <property type="term" value="F:D-ribulose-phosphate 3-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00429">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000074">
        <property type="entry name" value="Ribulose-phosphate 3-epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02227">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026019">
        <property type="entry name" value="Ribul_P_3_epim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000056">
        <property type="entry name" value="Ribul_P_3_epim-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004076">
        <property type="entry name" value="PRK05581.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01163">
        <property type="entry name" value="rpe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11749">
        <property type="entry name" value="RIBULOSE-5-PHOSPHATE-3-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00834">
        <property type="entry name" value="Ribul_P_3_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001461">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01085">
        <property type="entry name" value="RIBUL_P_3_EPIMER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 15" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="14 17" id="KW-0170">Cobalt</keyword>
    <keyword evidence="12 15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="17" id="KW-0464">Manganese</keyword>
    <keyword evidence="10 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 17" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="16">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="146"/>
            <end position="149"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="197"/>
            <end position="198"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00009541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00013188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00013920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR001461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OEJ80562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="228" mass="25141" checksum="94BB798BA2100D1D" modified="2017-01-18" version="1">MVKSHIAPSILASDFTELGCQCNKVIDGGADWLHLDVMDGHFVPNISLGLPVIESLRKKIPSSRCFFDCHMMIENPEKWVQKFADIGIDQFTFHYETTKNPKELIKLIKSCGLKAGCAIKPGTDVEVLYELAPLLDMCLIMTVEPGFGGQKFMKDMMPKVEHLRNKFPNLNIQVDGGLGKETIGDAATAGANVIVAGTSVFKAEKPSEVISYLRKEVDDNLEARGIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R153</accession>
    <name>A0A1E5R153_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cell division control protein 50</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4305</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the CDC50/LEM3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R153">
        <property type="interactions" value="378"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R153"/>
    <dbReference type="InParanoid" id="A0A1E5R153"/>
    <dbReference type="OrthoDB" id="340608at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005045">
        <property type="entry name" value="CDC50/LEM3_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926:SF0">
        <property type="entry name" value="CDC50, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03381">
        <property type="entry name" value="CDC50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015840">
        <property type="entry name" value="DUF284_TM_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="5 6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="402" mass="45474" checksum="E69042947D9D1336" modified="2017-01-18" version="1">MGILQTLHLKKQPNAITVDETHKSKKPPNTAFRQQRLRSWQPILSPQSMIPTLTVIACIFAPIGIALLIAVLRVQHVVLDYSQCDTLASTTGFSKIPSKYVHNYFRTSTDFDPEWKLVXASSDNXDTCQLKFEIPHDIGSPIYMYYKLTNFYQNHRKYVESYDLDQLKGKAVDADSLNDFCKPLRSENGKPIYPCGLIANSMFNDTLSTTLLGADSNTKDYQLSSKNVAWXTDRKVYKKTSYNASDIVPPPNWQKMFPNGYTEENLPDISQWQEFLVWMRTAGLPSFYKLSSKNEKDTLPAGTYTMEIELNYPVSYFGGTKSFVLTTNSVIGGRNVSLGVVYLIVAGVCLLFGAMFLMKVIFKPRKLGDHSYLNFGHNXDQEGDNSSARNLNEQLTPLRDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R182</accession>
    <name>A0A1E5R182_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Mitochondrial distribution and morphology protein 31</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3843</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in the organization of the mitochondrial membranes and the global structure of the mitochondria. Also required for mitochondrial distribution and mobility as well as for the maintenance of mitochondrial DNA nucleoids structures.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MDM31/MDM32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R182"/>
    <dbReference type="FunCoup" id="A0A1E5R182">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R182"/>
    <dbReference type="OrthoDB" id="17678at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012571">
        <property type="entry name" value="Mdm31/Mdm32"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31068">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31068:SF0">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08118">
        <property type="entry name" value="MDM31_MDM32"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="135"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ80668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="590" mass="68016" checksum="DB52526880F1AD1F" modified="2017-01-18" version="1">MFKGLLNAHTLTRTYRSTLGYKQLLPTQSTLPFTRHLSRTTRLHNELNGLSNKVHNKVHNKANNIATPHATHQNAKAAESLHANTNFSLLHPVSKPTKDYYLSQATSLWSRIKINTKWFLKKSMKPFNSDDISAFISWVVVSNVLIILLWTTGFTSLVLFIMNTVFAQEYLATKVGELITENTTNFLVVFENAIVPDWKGGNITFNNVFVSKRPINEEEKKNTKNFIKGSQREAVTRAKLALSQQQILVNEENFDEGNFTQYDLTIDQVNVSFSFTKWFSGKGIIDELELNGVRGVVDRTHIFWKPNDDPRNYLNVHAPGDFEIDKFTMNDVLFTIYQPNGFRPFKVSIFNCDLPQLRKHWFFYDFINANAISGTYDDSLFSIYKRYNKDHKLITTMRVDNLNVDHLNGGVKGPFGWITRGKVDMIGDVKIINKQKKNLILDFFNLKDDPFLNPKKFVKNLLKLSTSEQNEQMIINYYLKLHNVQAQVPLLTSELSYINNALIRPIVGYLNSSKAYIPIRCTIEKDVNDFAGSWTMYDSLLNDDMSKKVYEAFADYAVDEERRTIRLKRIGFWSLQALLQLLLVSFNTLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1B2</accession>
    <name>A0A1E5R1B2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">37S ribosomal protein S26, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4194</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1B2">
        <property type="interactions" value="142"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1B2"/>
    <dbReference type="InParanoid" id="A0A1E5R1B2"/>
    <dbReference type="OrthoDB" id="275227at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="entry name" value="Iron/manganese superoxide dismutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43595">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S26, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43595:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="2">
        <location>
            <begin position="215"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="279" mass="31806" checksum="05B270BABD6976A1" modified="2017-01-18" version="1">MLRTXSSQFKTTFSKRTVFTVPKLNTPHXIPTNIYSAEGFQTVWNQYQKFLSEKLTYATQGTQLESLLPLQLVLKTSKEPFNVKVFNYASGLHSNHXFIENILPSSSSISSVSSVSEAAVQDNSIEVASNSLIVRKLEEKYGMXWEXLKKKIITDSEQKIMGQGYIFIVEDMNKQFDIIYCNNYGTPYYKPRQQSFDLNGGSLKQSDYAAYSNLKKAVRSTNTNNSSKDLKDFSLPLVAINLWDYAYLKDYGSGGRSKYLAKVLDNLNWNVINKRLFTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1C4</accession>
    <name>A0A1E5R1C4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cell cycle arrest protein BUB3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g4091</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1C4">
        <property type="interactions" value="258"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1C4"/>
    <dbReference type="InParanoid" id="A0A1E5R1C4"/>
    <dbReference type="OrthoDB" id="10262475at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10971">
        <property type="entry name" value="MRNA EXPORT FACTOR AND BUB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="294"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="4">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="383" mass="43071" checksum="53A8191DAE5D9531" modified="2017-01-18" version="1">MQGSQQKAIALSQSFSFIRTLEIDETTGVMATAPTTHFLAQEQDIISSLDLNNDNVLTVTTWDGTVNVYDINRYQGNEMETIDASSIYSVTHEYPILSSYNNXTIDSAQWFFGDVQGNLLXGDLRKQNLXVQKITLXSQINDEXKAFSSAVAGVGICRLKGSQDRRSLITASWHGDLRVVDTRTLTTAAHTNTEKKIFAMDFNESSMKIXCTNPSNSVDIRDIRKLDKPYEVRESGLRDQVRDLKIMNGGEGYAQCSVAGRVTVDYFDESLQTKKYGFRCHRKNLKDVDYVYSVNSICFKPNSNIFFSGGSDGKVSMWDYQLKKRLKTLDSFNSQSVVKLACNEKYLVIACSDDHFKTDPVLTFVDKTSASMVLPSNLYIYSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1E1</accession>
    <name>A0A1E5R1E1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ATP-dependent RNA helicase FAL1</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">ATP-dependent RNA helicase fal1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g4217</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1E1"/>
    <dbReference type="FunCoup" id="A0A1E5R1E1">
        <property type="interactions" value="688"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1E1"/>
    <dbReference type="InParanoid" id="A0A1E5R1E1"/>
    <dbReference type="OrthoDB" id="3970396at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000849">
        <property type="entry name" value="ATP-dependent RNA helicase DBP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000031">
        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13 17" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="16">
        <location>
            <begin position="24"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="55"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="239"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="12">
        <location>
            <begin position="24"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ80718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="395" mass="44296" checksum="7AC8D8BBC147D68C" modified="2017-01-18" version="1">MSFNRADDLKLKFQKTKTKLRISSTFESMKLKPELLKGVYSFGFEAPSAIQSRAITQIISGKDCIAQAQSGTGKTATFSIGCLQVIDTNSNDLQAMILSPTRELATQIATVVSNLGDYMNIKSMALTGGKLVSKDLKNLQSGCNIISGTPGRCLDMIKRQVISTRKIKMIILDEADELLSETLGFKKQIYDIFAKLPTTVQVVVVSATMSKDIIEITKKFMSTDSVKILVKQDQISLEGIKQYKVELGEEAWKLDTIIDLFQVLTIQQCVIFCNTKKKVDWLSKSLTQQDFAVIAMHGDMKQEERDKIMNDFRSGKTRIMITTDVWARGIDVQQVSLVINYDLPDIIENYIHRIGRSGRFGRKGEAINFVTTQDASTLRKIEKFYSIKIKPLPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1F1</accession>
    <name>A0A1E5R1F1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Trafficking protein particle complex subunit 31</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g4065</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1F1">
        <property type="interactions" value="366"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1F1"/>
    <dbReference type="InParanoid" id="A0A1E5R1F1"/>
    <dbReference type="OrthoDB" id="3971120at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990070">
        <property type="term" value="C:TRAPPI protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990071">
        <property type="term" value="C:TRAPPII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990072">
        <property type="term" value="C:TRAPPIII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14943">
        <property type="entry name" value="TRAPPC5_Trs31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016696">
        <property type="entry name" value="TRAPP-I_su5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20902">
        <property type="entry name" value="41-2 PROTEIN ANTIGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20902:SF0">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="57"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="185"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="67"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="185"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="224"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="373" mass="40278" checksum="BD8BFAA8C8137AE2" modified="2017-01-18" version="1">MAKLQTNMGYDPTVGPKQAVPSVLQSDPLIQQQATDPNNIYNSMKAGDRLPLDYVSTNSTPGTLVPGASTSSSSLTASGSQTSTSLSASQSQAMSNEVSLSAYMFLLAEIISTMAKDHPAQEHEERIGALGYGIGVKLLELLKYRQSLPRNLGRSFFSTGANSTTASTTASSVPIIPLTAGATRSFSAKHTPGSTTTAAGQSSRGQRMVSSPGVTHYKSTLPQPVSSVPSKSLASLSLEDPEGPNSVTSLLLNNTTGQKISIMEKRFLKILDILQFLHGPXWKYLFGKQSDDLQKSGDKNNEYMIIDNEPLISQLCAINFVNYFICGIIQGVLEHACFPCKAVKAHIVPDEKHEFKTVYLIIFEDQVLEREKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1I6</accession>
    <name>A0A1E5R1I6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Vacuolar morphogenesis protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g4331</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Essential for proper morphogenesis of the vacuole. May exist as structural reinforcement on the surface of the vacuolar membrane and be required for maintenance against rupture by osmotic pressure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1I6"/>
    <dbReference type="FunCoup" id="A0A1E5R1I6">
        <property type="interactions" value="163"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1I6"/>
    <dbReference type="InParanoid" id="A0A1E5R1I6"/>
    <dbReference type="OrthoDB" id="428895at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097576">
        <property type="term" value="P:vacuole fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15858">
        <property type="entry name" value="SNARE_VAM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000058">
        <property type="entry name" value="VAM7p Vacuolar SNARE protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="PX" evidence="8">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="7">
        <location>
            <begin position="274"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="221"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="331"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="340" mass="39430" checksum="F08C24476D1B5ADF" modified="2017-01-18" version="1">MKRFNDFYTFKTELEKQYKMEIPYDFPGKINALDVDATSSSANRGSSSSSWLNVVNFYKGNRSTDPQVVGYRKRMLEDFLNDMLHDSFDTKWKYSKLTLDFLEIKMSDLKNVEKPLQTKLRGNSSFYKDLNTNSEDKDEWWNIYRSGKEILESETCNVSDIMRARLKLRKLEELLESDEKNSSRKSKKTKFLNALRKHMTERSLDLDKNNKSQDFSSNLQRLTGSQMDQPTLPFSSTSFDYGAEPESNTQDKDPSIIHPGRLLGTNREEYQSQRQVLKAQEQGLEDLHKIILNQKNLGLMINQELQSQNELVDLLSNDVSNTSSKLSGASRRAKKYNNET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1L7</accession>
    <name>A0A1E5R1L7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Synaptonemal complex protein ZIP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3964</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1L7">
        <property type="interactions" value="368"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1L7"/>
    <dbReference type="InParanoid" id="A0A1E5R1L7"/>
    <dbReference type="OrthoDB" id="4036563at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="90"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="294"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="546"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="631"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="95"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="125"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="866" mass="98149" checksum="0931214B414243F9" modified="2017-01-18" version="1">MSGFFRDARGLKPRNNIFDKIRGNAVSTSLHRCSEPXGSQXPXNQYXNVANNSCYNANSDFNNESYDKTVGNSFNDDXCLEAKFQSTVRQNXNKYQDRPETQEDLIEDNHRXTHNPGEVFGMKLNSSSSKHNASSENPTAHKEESSDNDDCEITDIKEFSNEPPNKPIDSKQGNSNKNNEYDGSADIGHKNGTSDTSVYSHSNEFLLEAFTNTQRMCSVLKSDLDKSKQENYLLVQKNDNYKDVVGKIKSKLIFLSTRMENMQDLSTKLTKEQEKHVLNSVEAQKQHTHMRTELNNVTKTLKEFQESYKKLQAEKKQLEDINLQKSLEVEILEKKILVKDNSLNCKLDKLMXAYHQDFNELKIDLSENGEKLASEVQERIFKNLXQNSKSITDNLSQLFLEKFMEKTKFLKDSNLKQSGTFERLNRDTEIKLAAYSKEITKKLNCIGSTASSNYSNLLEHINGSIVLRSEKLHELVMDQFSKSALSSQIFKDLLNEQLTEILQKTVDPLDFKNVLEKQATLTQENTSLTHKCNELQTEKAQHVVQIDESKNMIIELSRNVDIFKNKVDSQEQDFRAKLGDQANKLRNLENLKKTQDQVVELLEKDNKALKLDLATYSCDECLANSGIKNQVTVLEKQLQVIMQEKVEFQEKIFSLENQLENWNIQNNTHASHIVAKNAESTKNKRVATSTTATSEFELVSSAPELEVENTIFKAPINKKANLQVCVTALPREMNDATSGKTGKKMSTEMGSKRFECFDESADKNNDENKDDFFADQTLSFNTQLKTLLDTASKYKRKSNFSTGGGDVKFTNTECSSSVAGKKKNVGPKISMKTMAKNNGAGTYDVVKKANVTKCRGVKNKKVKAMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1W9</accession>
    <name>A0A1E5R1W9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Translation machinery-associated protein 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3860</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3 8">Interacts with the 40S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The SUI1 domain may be involved in RNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the DENR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1W9"/>
    <dbReference type="FunCoup" id="A0A1E5R1W9">
        <property type="interactions" value="1204"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1W9"/>
    <dbReference type="InParanoid" id="A0A1E5R1W9"/>
    <dbReference type="OrthoDB" id="277199at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11607">
        <property type="entry name" value="DENR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.780.10:FF:000013">
        <property type="entry name" value="Translation machinery-associated protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050318">
        <property type="entry name" value="DENR/SUI1_TIF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046447">
        <property type="entry name" value="DENR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005873">
        <property type="entry name" value="DENR_eukaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048517">
        <property type="entry name" value="DENR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01159">
        <property type="entry name" value="DRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789:SF0">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21023">
        <property type="entry name" value="DENR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="SUI1" evidence="10">
        <location>
            <begin position="101"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="46"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ80882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="203" mass="22687" checksum="9A0C2EF4377A922A" modified="2017-01-18" version="1">MSVAHVVYCGVCSFPIEYCEFSGKLKKCKVWLQEEHPELFTKFYGSESEGSADGADGSGDGVDGSDADNKEKKLEKSLKKLEIKEENRDKRELQKKLNSKIVIKTIERTKRKRVVAITGLEVFEIDMKKLSKTFASKFATGCSVSKNVEKKEEIIVQGDVADQVEVYIQKLLQEKGMETVKVEKISGKAEKKKKTEDGAAPAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R208</accession>
    <name>A0A1E5R208_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4288</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R208"/>
    <dbReference type="FunCoup" id="A0A1E5R208">
        <property type="interactions" value="217"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R208"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="128" mass="14447" checksum="722B00DFC5F5CC7F" modified="2017-01-18" version="1">MKAQQLSDSKGGFTNSSSKENKIKNFQEEILESTEEYVILKHDAQHLEQLTNSNKELLSNLSALYEKVSSTNGFDELRQTLLSNRALLKTIFAQDNSSSTANSCSTQLDWEHLFGCSKQELELLLSEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R271</accession>
    <name>A0A1E5R271_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Inner nuclear membrane protein SRC1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g4153</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R271">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R271"/>
    <dbReference type="InParanoid" id="A0A1E5R271"/>
    <dbReference type="OrthoDB" id="2503928at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071763">
        <property type="term" value="P:nuclear membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1180">
        <property type="entry name" value="MAN1, winged-helix domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044780">
        <property type="entry name" value="Heh2/Src1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018996">
        <property type="entry name" value="Man1/Src1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041885">
        <property type="entry name" value="MAN1_winged_helix_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47808">
        <property type="entry name" value="INNER NUCLEAR MEMBRANE PROTEIN HEH2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47808:SF2">
        <property type="entry name" value="LEM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09402">
        <property type="entry name" value="MSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="878"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="domain" description="Man1/Src1-like C-terminal" evidence="9">
        <location>
            <begin position="887"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="53"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="306"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="444"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="514"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="753"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="808"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1088"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="58"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="74"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="195"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="221"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="239"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="274"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="306"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="444"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="514"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="808"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="825"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1089"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="10">
        <location>
            <position position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1273" mass="144132" checksum="FD353BF9F93DCCA7" modified="2017-01-18" version="1">MSQSYLNDNFDPNKVNKANLRKILGSNNVHFSLSASKKDLVKVFNANIESIKNSKSTNDAEKQKPVGKAKEKSPNSSVPTSSSSSLTSSSSSTTAGSRTNDSPKKRKLRNKQSKLNNLRKKQDTSFGTQDNTPAEVIVLDDEDDDNEENNIDEDSGFDEIKANQGQATKGKGKNLHITLNFPSDTSASSRKRSRANAEENEQKFKSEESTPIASTIDKSDTPSLKVQLSNKKQKKEKSPNSVTASLDSDVSKSASTNEENLSDFPSSITNSRETPSKIESKNHKLEEHKSLVIDKFEDTGSLENVSTHSITSALNRSFSIPSGKDVDEKDRVVENSDDDVLPGSNDLMHEDDDAVNEENVELFKRDIDLEKMNVSEEFKQLLQKTRDNATKEKQDLKNTPSVGQMEKEHLDALQNVETEMPKTTTTKTKSGEADQIDNEVDVFAEKKDSHAEGKPLRKAEQAFDDQQNREAGTPTVEADKQKTTLQTFEKKLQNVGNEVNSFVDVLMQEVQETVEQPVQPVKPSDNTSSANSSSAQSIVEKTISKPVKEVEQLVSKGSTAMASLANVVMEEVEKTVEQPVEQSVEQPVEQCVEQPRETHLTEATKQTTLQNTTEKPIKRAGQLISEGSKAVGSFVDVVMDEVERTVEEPVXETPEQPIEKVSTSLKPVEENKQELSFTQDSDMEALEENAAMSSFVDVVMDEVEKAVEEPVKDEYYDKSKVSLQEQPVSQNSAESSTSFVDDALSMIETAIEQPVEDNDFETGPKPANKPVENKDSDITEGENLSFFEEVYQEVQNAIEEPVTPVLNLKKRKMSKRKSEKMDRPHKSHKSNKVSKKADSKRSSKKLKKTSGVATSTKKIRNFSIHLKLKKFIVRFAKFLYTLLLFMVFLVPVLYALWYREQQVAVGYCGHSNDAKSSKLEFKNMFGNPFAENGYLKNLGEYEFKFIDKVENWVDSTKPKCIPCPDDTAYCFPDLEIKCKADHIKKKTVKSLYGLIPAFFHDEGIYQCVKNHEKEQMVEEAVSKILKILEFKNAQKDCGLSKDDLEVGMDVEELKRIFLEARKPWFKIENFDDIWNKVLEHLKSEPEITWRQTSPAEPNSRTNRSKTLDQNEQAKEIVSKEKIFVRSKSLKYTSWNCKYEQFILSKVRENKIPISSAVILLSTYQVMKYKYEKYQREQAMVKEFTSKILRYLQINKRRSMHGQVVYPYLSSVQLRDILSSENESLSNKNKIWNKISQHLERNNSTVKTELIEFNGEIMKVWEWVGPLDLLEAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2J4</accession>
    <name>A0A1E5R2J4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LSM2-LSM8 complex subunit LSM8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LSM8</name>
        <name evidence="11" type="ORF">AWRI3579_g3853</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Plays role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">LSm subunits form a heteromer with a doughnut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2J4">
        <property type="interactions" value="442"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2J4"/>
    <dbReference type="InParanoid" id="A0A1E5R2J4"/>
    <dbReference type="OrthoDB" id="422364at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01727">
        <property type="entry name" value="LSm8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034103">
        <property type="entry name" value="Lsm8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044642">
        <property type="entry name" value="PTHR15588"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588">
        <property type="entry name" value="LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588:SF9">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ81127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="124" mass="14154" checksum="3214502D8936DCEE" modified="2017-01-18" version="1">MSLLKDYLDQKVVVITTEGYTLEGKMISFDKHCNLVLEGCKXRFAQAGTASNNEEHNNGPQVKLLRGSEVVCVGLVDEELEKTEVEKYVQENHGMTLEKLNDTKNKPPKRFEILRKVSDYKLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2K9</accession>
    <name>A0A1E5R2K9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Multivesicular body sorting factor 12 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3854</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R2K9"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032509">
        <property type="term" value="P:endosome transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019014">
        <property type="entry name" value="Mvb12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09452">
        <property type="entry name" value="Mvb12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Multivesicular body sorting factor 12" evidence="1">
        <location>
            <begin position="41"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="110" mass="12837" checksum="29E37B8472E9E407" modified="2017-01-18" version="1">MKVTIKEEYIVCEKDMVTMPPLIPSLYELEEQQTKQDQQSLDKENTLSVLKLWIKECDKFKKQLXXGGVSDPSKNVSESQSSFQEWYNQEFIKKQPPGLTMSLLDLQKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2N5</accession>
    <name>A0A1E5R2N5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Deoxyhypusine synthase</fullName>
            <ecNumber evidence="6">2.5.1.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g4021</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[eIF5A protein]-L-lysine + spermidine = [eIF5A protein]-deoxyhypusine + propane-1,3-diamine</text>
            <dbReference type="Rhea" id="RHEA:33299"/>
            <dbReference type="Rhea" id="RHEA-COMP:10143"/>
            <dbReference type="Rhea" id="RHEA-COMP:10144"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57484"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:82657"/>
            <dbReference type="EC" id="2.5.1.46"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; eIF5A hypusination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the deoxyhypusine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.46" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2N5"/>
    <dbReference type="FunCoup" id="A0A1E5R2N5">
        <property type="interactions" value="695"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2N5"/>
    <dbReference type="InParanoid" id="A0A1E5R2N5"/>
    <dbReference type="OrthoDB" id="294378at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034038">
        <property type="term" value="F:deoxyhypusine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.910.10:FF:000001">
        <property type="entry name" value="Probable deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.910.10">
        <property type="entry name" value="Deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002773">
        <property type="entry name" value="Deoxyhypusine_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036982">
        <property type="entry name" value="Deoxyhypusine_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00321">
        <property type="entry name" value="dhys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703:SF0">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01916">
        <property type="entry name" value="DS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0386">Hypusine biosynthesis</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ81144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="408" mass="45328" checksum="1AFE86C810FF30D9" modified="2017-01-18" version="1">MSPAETQKNNQELQNNEDTMSSSLNDVLPDMLKEAVLMESELPENSTIIKGVDYSKANATNMNAVDLVEAMKTMGFQASNLGKACEIIDKMRSWRGKHISELDETERKGKFDDDGYQKATIFLGYTSNLVSSGIRETLVYLAKNKMVDAFVATAGGVEEDLIKCLAPTYMGEFHWKGKELRSKGLNRIGNMIVPNDNYCLFEDWIIPVLDKMLDEQEAHVAKNGVEACLDATEDPDTPIWTPSKLCRRFGKEINNESSLLYQCYKNDIPIFCPAITDGSIGDMLFFHTFKASPKQLRVDLVSDIRKINTMSMEATKAGMIILGGGLIKHHICNACLMRNGADWAVYINTAQEFDGSDAGARPDEAISWGKIKADAENVKLYAEVTLVFPLIVAATFASGKKIENQKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R357</accession>
    <name>A0A1E5R357_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pre-mRNA-splicing factor SLT11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g4244</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in pre-mRNA splicing. Facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome. Binds to RNA.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R357">
        <property type="interactions" value="154"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R357"/>
    <dbReference type="InParanoid" id="A0A1E5R357"/>
    <dbReference type="OrthoDB" id="10259600at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048995">
        <property type="entry name" value="STL11/RBM22-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21369">
        <property type="entry name" value="STL11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="STL11/RBM22-like N-terminal" evidence="5">
        <location>
            <begin position="12"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="340"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00019060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF21369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ81003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="377" mass="42744" checksum="8A279DD4A182DBC2" modified="2017-01-18" version="1">MDQESTNAPFNLCSDCLPASGESPSITLYKDTDASHRCKLCTSYFTNYSNRKLGINGLKICPTCSKIKHLCQHCLLDIQLLIPMDLRDEIFAELGLDKDYKHLTDNMKNIKSEIMKRYIHDQNSKLLASDSPEKGTRSTNIDYANVVIPKIREKLDQTESLIQTDQNSDFPTKTVNKKDIQNFEHFKKVFSSVLDFSNSAKPASQISNTDQSANVCLLLFNISRQTAEWEILKSLNENYKIVNSQIVNFNCKQTGGVALLQCTASAQAAFRSAXSSSEAFFTMNMGKNNSKIYFTFISLSQYAQINKFLSNALYQELNNLNKFINFSMIKILKAKSQNLSHSVDTDRSNGNIKAEQKKQKHNKVTKKGKRIRHNLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4H9</accession>
    <name>A0A1E5R4H9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerases I, II, and III subunit RPABC1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3730</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the archaeal Rpo5/eukaryotic RPB5 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4H9"/>
    <dbReference type="FunCoup" id="A0A1E5R4H9">
        <property type="interactions" value="1105"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4H9"/>
    <dbReference type="InParanoid" id="A0A1E5R4H9"/>
    <dbReference type="OrthoDB" id="248779at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1340.10:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.940.20:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1340.10">
        <property type="entry name" value="RNA polymerase, Rpb5, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.20">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00025">
        <property type="entry name" value="RNApol_Rpo5_RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014381">
        <property type="entry name" value="Arch_Rpo5/euc_Rpb5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005571">
        <property type="entry name" value="RNA_pol_Rpb5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036710">
        <property type="entry name" value="RNA_pol_Rpb5_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000783">
        <property type="entry name" value="RNA_pol_subH/Rpb5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020608">
        <property type="entry name" value="RNA_pol_subH/Rpb5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035913">
        <property type="entry name" value="RPB5-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007129">
        <property type="entry name" value="PRK09570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01191">
        <property type="entry name" value="RNA_pol_Rpb5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03871">
        <property type="entry name" value="RNA_pol_Rpb5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000747">
        <property type="entry name" value="RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53036">
        <property type="entry name" value="Eukaryotic RPB5 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55287">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01110">
        <property type="entry name" value="RNA_POL_H_23KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase Rpb5 N-terminal" evidence="8">
        <location>
            <begin position="7"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase subunit H/Rpb5 C-terminal" evidence="7">
        <location>
            <begin position="142"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ81815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="215" mass="24920" checksum="CFC070987DBD2D57" modified="2017-01-18" version="1">MDGDNDRGVSRLWRTYKTVKEMVRDRGYFISDQEIELSLEDFKNKYCDSMGHPQRSMMSFQSNPTEEALEKFPKMGSVWIEFCDEASVGIKTMKNFVIHITEKHFQTGIFIYQNGVTPSAMRLLPTAAPATIETFHEGSLVVNITHHDLVPKHIKLSVEEKQELLKRYRLKESQLPRIQRMDPVALYLGLKRGEIVKIIRNSETSGRYASYRICL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4K2</accession>
    <name>A0A1E5R4K2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Carbamoyl phosphate synthase arginine-specific large chain</fullName>
            <ecNumber evidence="13">6.3.4.16</ecNumber>
            <ecNumber evidence="3">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Ammonium-dependent carbamoyl phosphate synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Arginine-specific carbamoyl phosphate synthetase, ammonia chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Glutamine-dependent carbamoyl phosphate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">AWRI3579_g3549</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>hydrogencarbonate + NH4(+) + 2 ATP = carbamoyl phosphate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18029"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Heterodimer composed of 2 chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CarB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.16" evidence="13"/>
    <dbReference type="EC" id="6.3.5.5" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4K2">
        <property type="interactions" value="454"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4K2"/>
    <dbReference type="InParanoid" id="A0A1E5R4K2"/>
    <dbReference type="OrthoDB" id="1924069at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004087">
        <property type="term" value="F:carbamoyl-phosphate synthase (ammonia) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000026">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000002">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="entry name" value="Carbamoyl-phosphate synthetase, large subunit oligomerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036897">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006275">
        <property type="entry name" value="CPSase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CPSase_lsu_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058047">
        <property type="entry name" value="CPSase_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01369">
        <property type="entry name" value="CPSaseII_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003671">
        <property type="entry name" value="PRK05294.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009455">
        <property type="entry name" value="PRK12815.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF53">
        <property type="entry name" value="CARBAMOYL-PHOSPHATE SYNTHASE [AMMONIA], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405">
        <property type="entry name" value="CARBAMOYLTRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25596">
        <property type="entry name" value="CPSase_L_D1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="Carbamoyl phosphate synthetase, large subunit connection domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="10 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ATP-grasp" evidence="22">
        <location>
            <begin position="167"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="22">
        <location>
            <begin position="718"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="23">
        <location>
            <begin position="986"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00074189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="OEJ81846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1156" mass="128375" checksum="286FAB4E72D00374" modified="2017-01-18" version="1">MTVDSSNNNSSTPINPIYGNRQPTTSAFNFDTYKPDLIKDVNTVLVIGSGGLSIGQAGEFDYSGSQAIKALKESNIKTILINPNIATNQTSLDLADKVYYLPVTPEYITYIIEKDRPDGILLTFGGQTGLNCGVKLDQSGVLAKYNVKVLGTPIKTLITSEDREMFASALKEINIPIAESVACSTIDDAIHSAKKIGFPVIVRSAFALGGLGSGFADNEEEMIKLSSQALSLSPQILVEKSLKGWKEIEYEVVRDSADNCITVCNMENFDPLGVHTGDSIVVAPSQTLSDQEFHMLRSAAIKIIRHLGVVGECNVQYSLQPDGLDYRVIEVNARLSRSSALASKATGYPLAYTAAKIALGYTLPELPNPITKTTVANFEPSLDYIVVKIPKWDLSKFQYVNTNIGSSMKSVGEVMSIGRTFEESFQKAIRQIDPQFLGFQGSPEFKDHALLDEVLANPTDRRWLAVGQALIYENYTVDRVHELSKIDKWFLYKCMNIVEMYRILESLAHSNKGGATSGDLSSLNSETLLKAKKLGFSDKQISLIFEVENELVVRKHRLSMNIKPFVKRIDTLAAEVPASTNYLYTTYNATKNDIEFDANPYLVLGSGVYRIGSSVEFDWCAVNTAKALRNMGEKIAMINYNPETVSTDFDEVDRLYFEELSLERVLDIYELENPKGTIVSVGGQLPQNIALKLDDYGVKICGTSPKNIDNAENRHKFSSILDSINVDQPTWKELSHYEDAQNFATNVAGYPVLIRPSYVLSGAAMSVVHNEHELEEKLQNAADVSPDHPVVISKFIEDAQEIDVDSVAYKGELITYAISEHIEDAGVHSGDASLILPPQSLCKTDKLRLKEIAQKVAKAFEITGPYNMQIIKDQSGNLKVIECNIRASRSFPFVSKVLGVNFIDLVVRSFMDDVAVLNEYKNKDLMIDQEHKYNYVAVKSPQFSFTRLAGADPFLGVEMASTGEVASFGKDVYEAYWASIQSTMNFKLPLPTTSNDGHSTDKPGILFGGDLTKPFLKEVFDIYQSIGLYELFAVTPDTVDYLQSDNVKLLEFPHDIGKRXLRDIYFGEYNIQGVVNLSSAKAENTQDPDYQMRRNAIDFGLPLFNEKRTAKLFAECLKLKIQEKLHTLESIDINNDKPVVIPNEVVSWNDYLATLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4K4</accession>
    <name>A0A1E5R4K4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Intrastrand cross-link recognition protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3596</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4K4"/>
    <dbReference type="STRING" id="56408.A0A1E5R4K4"/>
    <dbReference type="InParanoid" id="A0A1E5R4K4"/>
    <dbReference type="OrthoDB" id="3972244at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22011">
        <property type="entry name" value="HMG-box_IXR1-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF22">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION FACTOR A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="4">
        <location>
            <begin position="295"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="HMG box" evidence="4">
        <location>
            <begin position="368"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="2">
        <location>
            <begin position="295"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="2">
        <location>
            <begin position="368"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="121"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="236"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="16"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="246"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="282"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ81847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="442" mass="49510" checksum="5BE11C7F0542888E" modified="2017-01-18" version="1">MNQGIKTSPGSKDQDQLQQQQQQILQQHFQSQQQQQQHPQLHQNNANVLGSTNGNTNHNLMGSQLSGSGAFGHVSPGSLPQGVDANGLTQQQLQHYQQLQQYQQLQQLQQQQQAQQQAQQQYQQQSQQPHMQQHYQQSQQGGAGPGNAANNINNMNITEAMFNSFLSQISQRSNPNMMAQQGSPNAAAAMSTQAAQFQQPNAAQQMNFPRQFHNTGAPFLDATAAFMQPQATKYAGKQEAGTSRGPRGGAAAGGANGVRVSGTSKKNAAAQAEVKKLSASQKRVEKRKELVKQGPKRPSSAYFLYYQEIREKLKADNPEMKVPDLSKMAAEKWRAMTEEDKKPYQDRVAGLWSTYRINKAEYESKLPPKKPSGPFVQFHRDIKKELQGDNPGKNLLELTKLASERWKNTTPEVKKKYTDAYKEQMQEWKDAYERLDPAEETE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4R3</accession>
    <name>A0A1E5R4R3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Regulator of drug sensitivity 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3841</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4R3"/>
    <dbReference type="FunCoup" id="A0A1E5R4R3">
        <property type="interactions" value="228"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4R3"/>
    <dbReference type="InParanoid" id="A0A1E5R4R3"/>
    <dbReference type="OrthoDB" id="2943660at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF29">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="11"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="59"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="64"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="99"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ81899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="875" mass="99938" checksum="CD8D5B957357209A" modified="2017-01-18" version="1">MRKSRNTTVTVCNTCRSRKIKCDRKKPTCTRCEESTLTCTYGRANRLSPQQLPSIHDQSVFPESQQSSSTQLNPSPSSFPSLHNYKIQPPLLPFPQIPRQTNPQTPSSFSVSSNQTDSTILNMNSVSSASTYDSSSSKVSTHSGEHVFITIKDYPKPVVMILEDSKYFSEPSVVFAFGHKDPFLKAFAGSVYGLTSLEYSLGKNEGVKEWLESIKFTDGDVKEISLIKFVEKIIKRNEQNSKRSKPEMWTPSILFWNDFKLQNELSDGVLPPFLVNLLGDVEKAIPEVTLVQELLQHFYDVIYPVHPYLNLPSFKNTIAEVLVADATFYKIDLGSTGIRRKIVTLALLLIILRISHVSIHVSNDVYLKEKFAAAKTITGTTVVLAQKLYSLLNTMETSNEDGLVFLLYLNVSEICDPENEHVCLLQKERLILNSIRDYTSFLGLQKDMNTFPHIAALKEFGDNYIFQRKKLYVGATVWRLNRLLLDGYGNIQEILDVEQFIQQKHGIIVDPAYNFMFKKLRYYLLLLKLNNVANNEINVPLRDFKKLLNNCENMLAKEFSLGQLAEQSTTSDKQFACEIIALNITGKICVLNQTFLLIIYFEDKVSFDPFEFSQYQVEFMLDSFRILVELLGMITKYLEGSYDVYVPKSQAYMLKPLICEALLKCWVFLVAMIIRLKYCEKNLKALLISQSMFYQSSASNNSRNGESAKKALIQKLELLMGNCTELLRYSVHLSAETIAKSHYSCYESTCVLGYVVQLLDMKKLTEATERFWTLLNFVPPEIINKIQLKWGIEISETTVILNKLENSNVLNNIDENVVETMDRILSDIDFSYKNVNLPTPYISDNSAIAQFLQNDLDEYFDFWDDNLPALINPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4S2</accession>
    <name>A0A1E5R4S2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinetochore protein NDC80</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g3574</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the NDC80 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Chromosome</location>
            <location evidence="10">Centromere</location>
            <location evidence="10">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the NDC80/HEC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4S2">
        <property type="interactions" value="317"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4S2"/>
    <dbReference type="InParanoid" id="A0A1E5R4S2"/>
    <dbReference type="OrthoDB" id="7459479at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.30:FF:000001">
        <property type="entry name" value="Probable kinetochore protein ndc80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.30">
        <property type="entry name" value="Ncd80 complex, Ncd80 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005550">
        <property type="entry name" value="Kinetochore_Ndc80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055260">
        <property type="entry name" value="Ndc80_CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038273">
        <property type="entry name" value="Ndc80_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10643">
        <property type="entry name" value="KINETOCHORE PROTEIN NDC80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10643:SF2">
        <property type="entry name" value="KINETOCHORE PROTEIN NDC80 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03801">
        <property type="entry name" value="Ndc80_HEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="9 10" id="KW-0137">Centromere</keyword>
    <keyword evidence="2 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="4 10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinetochore protein Ndc80 CH" evidence="13">
        <location>
            <begin position="71"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="61"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="343"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="501"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ81871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="642" mass="74695" checksum="8E74FE6BC896E425" modified="2017-01-18" version="1">MNGMETNNNIINSNTKRHSLLMSSNRSSLRQSQQRYSLLPSQTNNMRSSIAVRAYGNXAGPTXSATSTSTSNNNNYNNNNHSINSSSRRDLRPLGDKNFQNLIAQDILDYLNKEQFSIETNYPVSLKTFKQPTQKDFVTVFKWCYHRLDPGFSFVKTWETDLYTILKTIKYPYLNSITKSQISAVGGSNWPKFLGMLHWLVQIMKSLDMNLIGIDQNICDQNTQDMTILTNHNSTTLLGKEDQELKMIKYESMAEKVFIEFIIESYKNFLQMNDDMSKIIPKLEKNFDKFTKIIETDTLIIDELNTNLKIKFSSIMENFVKLNDLKTNNLILQKDYNKVDNFVKLMQNKKNEWTVKLEKIDQEIELKHVNIANLKEKIGQYEKELDQKGFSIEDIQKKMELQTEISKNLKSIESNLEMMQNSQKSTNLEYKKLSNSLENSIMKYNFTLESLNGTGNGLSNILPTEVSFQQLQLSMDTISMEKIQSINKLLNSWNNEINGNIVTLQQKNLQIESQIESLQTEIENKNVYITDLENQYLMTKNENEITMQEFETQILEKQCDIEKLENKITELNQQVNDYLHDAQREIKSKEQEYKELSSNCEKNEKEIVYKVKEIVKYVKEFQNAFKQGLNEITPSPSETSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R503</accession>
    <name>A0A1E5R503_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">F-box protein SKP2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3751</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R503">
        <property type="interactions" value="78"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R503"/>
    <dbReference type="InParanoid" id="A0A1E5R503"/>
    <dbReference type="OrthoDB" id="4032719at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="67"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ81948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="746" mass="86402" checksum="E0734F1C48B16002" modified="2017-01-18" version="1">MKKFQLLGISSSRHKHSNKHKTRTDEASSSLLGQFSSSTESLLSVQSSHESQKKHHMRRYTFPAAESLHLTKLPEKLFFYIIDLLADDIPTLLALCLVNKQFSELVSRKYLYKHVVMHSKLSLLKFNISIHSNENAKKRFSVNKIEFLNPECQDSMLKYGRFYKKSSTPVMAGSFEIQDSIPTLHSAKNQVKSGNVFSSSSSSTSSYFSGLFSRPENEKLLHKYSRYTYIELLLDIIDYLPNLEHIALNDIDPGFMIPPWYSVLNDGTKEFFNKILKNQESMTRDDLKSFKLSKEWIENYQTKYYSLPRYKTLELVGRTKQMANCLLKPFYFYAFGVFEELILENIDISSQSLECPFELLPLQMKLDKTGYLDIHSPVSDLTLNNCNIVVNHGIFKNLHDYFSLVRSLTLLNLKNSYDLLLSNCFPLLQNLTIDCNSLCFISDESVKDSYYLASKQKKQADLQRNDAENHNFNGNGNEDAADDMVSLTDTLIDPLETRSFIKYPPPTTPVIVTYDLASTNLNKINMKDTEKGGKVTKHQLDFFRSLNISNFYLNYHKFHKLWMKLPNCSNINVKIVNIPFTNVFPVSPSKYLDFLQSLXTDDQVTVSAQPLLLNEEDKEYWWEESIKKCFTQLMSNDAVVENEDASSILDSTIINGYRDLCNYKDIPNINLWFFLTTLSKFKSVEIHMLRQWLFCTPRSRYDWELLLRPLLNGETPIKVRDRCGYVLYSYGCNKTKNTFKNTKTIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5D4</accession>
    <name>A0A1E5R5D4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Low-affinity iron/zinc ion transport protein fet4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3624</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5D4">
        <property type="interactions" value="263"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5D4"/>
    <dbReference type="InParanoid" id="A0A1E5R5D4"/>
    <dbReference type="OrthoDB" id="2224262at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007251">
        <property type="entry name" value="Iron_permease_Fet4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04120">
        <property type="entry name" value="Iron_permease"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="369"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="403"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="479"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="509"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="571"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="590" mass="68559" checksum="439D63BC556E5A69" modified="2017-01-18" version="1">MQDHNKAQYVMKEDILVNEKKYDFSKATDSLDNVSNFDTPPAXKLSLWRKIKIGMRPNTLDDVHARSKTLTEEEHLNLENENPLTYGQHFLQHKPSKFGRTFDRLTNFAGDTVVFLITCAILVMWMILGIVYRAPDNWQIAMQDGSSIQAYFSDSFLMRQQQNEYMDLLQFLCEFKKNAXNYLKIFEHYNQTKXKANSPTFKPLEQEELTTIEEELNDVLGDAHKMQTYNWFDRLTDFASKIMGSFYTTVLYWIGIFIWIGLGALPYLHFADEWQLYINTATAVVITFASMFIQNIRKRHDRYLQRSLSVILHTDADIDYKLRNIAGLYENDEESIVGDSSSANTSIDDQSYNTIKAKPLNKRVRAIEYYAHFVGGGVGAMISTCVFVVWICIGKPMKFSDNWWLIIGTYTGLVGFVDGFTLRSSYYRYLRYCMKQIYELESLEEKLLTYLHLPVTKTRILTNKERSFNYRASAWLGKWISYSWGVVASIFIVIGLIVLASGMRWSTTAQLICNTPTMIVEGFCLLALLEGHNQNNEKLRILAHDCLVRKLYLKTYVDHFLQHQEKHDDEKKTSVEIVTNERDYSNDSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5D7</accession>
    <name>A0A1E5R5D7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3541</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R5D7"/>
    <dbReference type="OrthoDB" id="435881at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050815">
        <property type="entry name" value="TF_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47338:SF5">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47338">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="151"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="813"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="813"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="826"/>
            <end position="836"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ82111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="873" mass="97691" checksum="26767D9733B8AB04" modified="2017-01-18" version="1">MSELQRTSSNNNNQHHEDDRSNVDIALFEQDSGDVDVEHTDDSEETRXKXRKNTTSTSRRRTSGEDDDISENLLNIHQGKEPDSSTIQVSVIHPSEGGKQHRESLVSKANKFDSQEAKLTRGINDKESDSKIFVSQGSQPHMDVDGMSIISCVNCRNRKVRCPRDLPSCRTCLKRGQLCVYPATKRTRSTMKRSTQDAFLKNENFYTMKVELDNKKINGAEPTQDQESSTDAELAAAVAQQAQEHAAVAAALNAAASNERTSLPPSKIDSAALETDSFGVLPASGNQKVKKSHSKHNQMAKLINAIYSKHPLVHLGNVNLKKIVKSPNFVVIDSLSQQQLQNTTSSTSKDSXFMSWKESGAPPSLFPLTNISPVLAVHSGNTGKSSLSSVPSSDSLKTALDLPETFSNPFTMTDQENEDGSFVGKILSKEFVKKISSQIEADKDLYQNPTDDHDSNSVEVKPKNENLQAKNAKLKFFKELAHNDFFAKYNPPSIDSKDPEFSILLFTEYCLNSFDFKHLDEFLYEMDDLKHPHPIKVVGKLIVVRSLLSEKALSKQNVQXCYSLLQSVSNELFQLQSDRVAYINGKVKVASFFLRNIWESFTLLDFIVSQLLLNRKMITKVSQCFSESALPEFLIALNSIPLIADNNCVNEAIELDGKLDNLLSSMSSSSSTKLYRFFLIVAKIYAVLPHSFSNLYLNMSMQRSSQLFFNMLLQDFTIFSDDKSAEYKYIFPVIAQILQTVTXHLSYQLLDVKEKSELVGRSAVVNSLDLLQIFLENIPPINTLPPYLYKPLSVLNESFIPLIENEPLSELSTAQSDQASXSSEIPAKKDADKTGQEKPASLLSSLNTDEPLNKLVLFYSRYPKSFWNHLCFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7D4</accession>
    <name>A0A1E5R7D4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Phosphomevalonate kinase</fullName>
            <ecNumber evidence="3 13">2.7.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g3483</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(R)-5-phosphomevalonate + ATP = (R)-5-diphosphomevalonate + ADP</text>
            <dbReference type="Rhea" id="RHEA:16341"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57557"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:16342"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the GHMP kinase family. Mevalonate kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.2" evidence="3 13"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7D4"/>
    <dbReference type="FunCoup" id="A0A1E5R7D4">
        <property type="interactions" value="125"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7D4"/>
    <dbReference type="InParanoid" id="A0A1E5R7D4"/>
    <dbReference type="OrthoDB" id="10262935at2759"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00099"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004631">
        <property type="term" value="F:phosphomevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000101">
        <property type="entry name" value="Phosphomevalonate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016005">
        <property type="entry name" value="Erg8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035102">
        <property type="entry name" value="Phosphomevalonate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01219">
        <property type="entry name" value="Pmev_kin_ERG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31814">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31814:SF2">
        <property type="entry name" value="PHOSPHOMEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017288">
        <property type="entry name" value="PMK_GHMP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 13 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 13" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10 13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="11 13" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="14">
        <location>
            <begin position="129"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR017288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ82791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="440" mass="48022" checksum="B7DD6B3FCA70DC06" modified="2017-01-18" version="1">MAKAFSAPGKALLAGGYLVLDPKYKAYVVALSSRMHAVVEHAPSNESRVTVESSQFNNDRWSYKIEKNGAGNFIPVEANNLQNPFIEKTILNVFNYSQPENGIDIKISIYSDAGYHSQANSVVKKNQYNSFNFYKETVANVPKTGLGSSAGLVTVVTSALLSCFLQNFDLKSTETQTCIHNLAQVSHCQAQGKVGSGFDVAAATFGSILYQRFDPALINDLPDVSSSAEYKETLNKLVSKTSWKFSAEKINLPKGLKVVIGDVNNGSETTKLVAEVKEWRAHNGSRAKDVYNCIDEGNMRFIQGLEQLNELAEKDPESYTNVIKSIDTKRTVNPALLNIITGVFQARGNFRIITQESGAQVEPMAQKTLLDSATELPGVLTGMIPGAGGYDAIALITTADCPIHELAKENDFLANVTFLDLKQESAGLQEEDQAHYTNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7D7</accession>
    <name>A0A1E5R7D7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">Pre-mRNA-splicing factor PRP46</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 7">Pre-mRNA-processing protein 46</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3417</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in pre-mRNA splicing and required for cell cycle progression at G2/M.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the WD repeat PRL1/PRL2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7D7">
        <property type="interactions" value="999"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7D7"/>
    <dbReference type="InParanoid" id="A0A1E5R7D7"/>
    <dbReference type="OrthoDB" id="10256122at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045241">
        <property type="entry name" value="Prp46/PLRG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19923:SF0">
        <property type="entry name" value="PLEIOTROPIC REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19923">
        <property type="entry name" value="WD40 REPEAT PROTEINPRL1/PRL2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="1 6 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="83"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="130"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="172"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="215"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="366"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="8">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU369036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ82822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="403" mass="45085" checksum="F55BD6BE3A7F4C01" modified="2017-01-18" version="1">MNNISADDVYMKAKQSFDFAPYKKVLSKEIDTAFEQVVXEPALGNDVTHTSTTDNKLIDREMTELTLSQNGTPSMDWELQTMLGGHNGWIRCLQVDPVSNEWFASGSADSSVKIWTFDPNEEGRHLAATLQGHVMGVRDIEISVHNPYLFSCSEDKMIKCWDLENNQIIRDYHGHLSGVYCVVQHPSIDQIIISGSRDSTAKVWDVRSRSDVLTLVGHKQAVNKVLAFPVEPQVVTCSNDNTIRFWDLRXGGKPLKIITNHTKHVRDMAKNTQEFSFASCSTNSIMSWAMPAGQLLTTFEPQPVKPVPNNTWQEEPLGVVNTLSVSAKNILFAGTDSGDMVFYDYLSSQRLCQRSSIPYNNLNNTTEESDAGILTSSFDNSGNILLTGEVDKFIKIWRNISVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7L4</accession>
    <name>A0A1E5R7L4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Exocyst complex component SEC10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3147</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7L4">
        <property type="interactions" value="814"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7L4"/>
    <dbReference type="InParanoid" id="A0A1E5R7L4"/>
    <dbReference type="OrthoDB" id="125856at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009976">
        <property type="entry name" value="Sec10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048627">
        <property type="entry name" value="Sec10_HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048625">
        <property type="entry name" value="Sec10_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12100:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12100">
        <property type="entry name" value="SEC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07393">
        <property type="entry name" value="Sec10_HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20667">
        <property type="entry name" value="Sec10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec10 N-terminal" evidence="7">
        <location>
            <begin position="110"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex component Sec10-like alpha-helical bundle" evidence="6">
        <location>
            <begin position="273"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="38"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="598"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="51"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="604"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="617"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ82902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="940" mass="105724" checksum="8859B94246F96562" modified="2017-01-18" version="1">MNSLYELDPKWKKLLQLDNFLGGLTVHEFVQGLCQTQNNNGTGMGGRRAVANATSGTNGHNNNNNNTSNNSNNSNNNNNNNNNGLGTTTTSAASTGGAINVNTKLDNLDPKPYIRTLESTLNELQKLEFECGQKTNTLKKQVTHSELEHSEKVLEIVPEFQNLTADFNALDNGLSKVNHVVSPLGSKLEKNIRSKNSYIESVLLIQYYQEFYTLNNQTSQNGVQSSLANVDGAQTGLNGHTEDIHTQSIDKIPQNFQKFDPNNILDWKLGAKMATTLKNLLILAHKLETESLPKTVKTTKAIEEFAKNLESQLLENFNXSYRVSDFKKLNEIALILNYYNDGQNVIQSFIAQHSYLKEQLKMHEQNKNATLIELDEKTVQKINDPDEHTNFYESQISQYLEDLATVIAKESTLVYKVFEDKTHQVMLLFLQTIFQEIIDPKVDNLLTVCLSLSNLAYCRMLNTLYSLFQEFLKELQEFFIGIDKGKLASINTILDTCCQAIFNKYILNRSKYFDIEKRSLETILFAKTATFNSLNENKIKQKLLEKDVTNKMASFSNGGNNNLNDFVSSSTTTGTLTNTAQRKFQSLNTFFKTHLDRSEKTTTDDSTFNNGNITQRANSSANNASATPDQAREFSLSLVDAMLKCFVESLARIMELIPQKCGEYSVEIVDLLLSGVVPQYIESALEVAYYDLTKSNFATNGNFTISCFSIISKCSEMLNLLSISIKTIIIPILNNSPAYKKQVIGMTNGNFKKTEVMINNILEKLVNLCKIKIADSLLKQKKKDYLLKDTDIMDQEATLPVVEITHFLSSVYKNAVSSGFDGANLSTFLCKVGHDLYLQLLDHYRKFPVNSSGGVXVTKDIIAYQSMIEKWGLKEPRLQDDFATLRELANIFVVQQNLVSSLIKEGHLVDVSPFILGEYIARREDYLNSGVLTRLKIGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7N8</accession>
    <name>A0A1E5R7N8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">DNA repair protein RAD1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3228</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7N8">
        <property type="interactions" value="947"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7N8"/>
    <dbReference type="InParanoid" id="A0A1E5R7N8"/>
    <dbReference type="OrthoDB" id="361020at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000110">
        <property type="term" value="C:nucleotide-excision repair factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000736">
        <property type="term" value="P:double-strand break repair via single-strand annealing, removal of nonhomologous ends"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901255">
        <property type="term" value="P:nucleotide-excision repair involved in interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20078">
        <property type="entry name" value="XPF_nuclease_XPF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10130:FF:000009">
        <property type="entry name" value="UV endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047520">
        <property type="entry name" value="XPF_nuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150:SF0">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC4" evidence="11">
        <location>
            <begin position="860"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="67"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="572"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1011"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="78"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1034"/>
            <end position="1050"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ82914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1130" mass="129336" checksum="39D5BCC33951A53A" modified="2017-01-18" version="1">MSRGKLFVENSSEEEDENEELLAANELTFSNSQVAQRHLTQKDEVGKKDSNDARLLDDPFFPTLAAQDLDDLQSSRNENTKDDMKVRAVSDEELENAETAGNEDARRPTFDIKDIKPVDVQLSITVPFQQLIVQDMLVSEDPLLIMGKGLGTEVIVASLLYILSTPTNFANNVKKQSLVLLLNCTAEDNLKFQEEMMEISWLSEDQQARDFYIVSSETSSIERRRKLYLQGGIISVTSRILIVDILSGIIDLKTVTGLMILHVESLTNYCNISFITEXYREVNKWGFIKGISDSATSFVTKGFQPLHSKLKQLKLKNPILWPRFHVQISQSLNNSESKVIEVRVAMTENMTQIQFGIYECLKKCLDELNRKTTDLEMNDYWTLDNVLDSNFMRSMAAILEPQWHRISFETKQLVKDVSTLRKLLSYLLTYDALDFYEIIKMILEANKPSITRKYIESLWLLCDESQLVISNAKRRVSEQGKYVLEPQPKWEQLLAILDDINYERSNSVIKNESLVGPTLIMCSSNYTATQLSQIINLQNKESIRKHMMQKLELFLGTXTNNNKVRKAVKDLKEKSATVHTMGPADEEEXNEYSPAPSVRQEDLSGIDPNEMIDNETNLSVTFTRGAIKEHEKVRNSKRRRTRGAAYVAAVNKLKDKTNGEDIENLMDVSEIKKEIEQLGDYIEIDSEDDEDGLEEPVKLEKSETLFIQNEKNVWESRIDSFEYVDKNDQIVIEKFFNKNSESVLEELMPSYIILYEPDLHFVRKVELYRAVHPNTKIYFMYYRDSAEEQHHLTEIKLEKEAFSKLIKERVSLAKHFETDEDLSRYKNLVKMNLQLSSGKASTRIAGGQNFKGSSTINEMCVVVDTREFNASLPGLLYRYGVKVIPCMLTVGDYILTPNICVERKSISDLIGSLKNGRLVDQCKKMTKHYKHPTLLVEFSDNQSFSLEPFSETRRIRKINNSHPIGSLLMQDEIQRSLAKLVMRFPSLAILWSSSPLQTVNIILDLKQGREEPDPQTCVEVGKKASKQKTKVGKTSNTETSNESLSHTNSTLREDEESDAEDLFSAIEDENNLKKLLDVPGLANVDYYKIVSNVRDYNEISKMSLRQLADIIGDIEVAERVSAFIRNDLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7Q2</accession>
    <name>A0A1E5R7Q2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nascent polypeptide-associated complex subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3238</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Part of the nascent polypeptide-associated complex (NAC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the NAC-beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7Q2"/>
    <dbReference type="FunCoup" id="A0A1E5R7Q2">
        <property type="interactions" value="1189"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7Q2"/>
    <dbReference type="InParanoid" id="A0A1E5R7Q2"/>
    <dbReference type="OrthoDB" id="8033832at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005854">
        <property type="term" value="C:nascent polypeptide-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006613">
        <property type="term" value="P:cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22055">
        <property type="entry name" value="NAC_BTF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.30:FF:000001">
        <property type="entry name" value="Transcription factor BTF3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="entry name" value="Nascent polypeptide-associated complex domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039370">
        <property type="entry name" value="BTF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10351">
        <property type="entry name" value="TRANSCRIPTION FACTOR BTF3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51151">
        <property type="entry name" value="NAC_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NAC-A/B" evidence="6">
        <location>
            <begin position="35"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="124"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="124"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ82942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="157" mass="17002" checksum="E2700841791DB1B8" modified="2017-01-18" version="1">MPVDPAKLAKLQKMSGTNKVGGIRRKQVSKENTSKKDDTKLLNTMASLKAVNMSDVAEANFFQENGQVLHFDKVGVQVAAQNNTTAFYGMPKEKQLQELFPNIITQLGDDSLNALTQMAQQMEAAKKAQAGAEANPAETADDGIPSVEGKTFEDDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7S7</accession>
    <name>A0A1E5R7S7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Elongin-C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3247</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SKP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7S7">
        <property type="interactions" value="662"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7S7"/>
    <dbReference type="InParanoid" id="A0A1E5R7S7"/>
    <dbReference type="OrthoDB" id="249087at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000035">
        <property type="entry name" value="Elongin C transcription elongation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039948">
        <property type="entry name" value="ELC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001232">
        <property type="entry name" value="SKP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016073">
        <property type="entry name" value="Skp1_comp_POZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20648">
        <property type="entry name" value="ELONGIN-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03931">
        <property type="entry name" value="Skp1_POZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00512">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SKP1 component POZ" evidence="5">
        <location>
            <begin position="3"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ82969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="103" mass="11758" checksum="04446ABC740990B4" modified="2017-01-18" version="1">MKEVVLVSRNGEEFPISTELAKISPIIKADIEMSTSKXNKPVVIELKEIESNVLSKVIEYLKYKYEYTKNDDEYNEDADDIPAFDIPVDMSLDLLIAADYLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7W3</accession>
    <name>A0A1E5R7W3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Mannan polymerase complexes subunit MNN9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3253</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ANP1/MMN9/VAN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7W3"/>
    <dbReference type="FunCoup" id="A0A1E5R7W3">
        <property type="interactions" value="115"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7W3"/>
    <dbReference type="InParanoid" id="A0A1E5R7W3"/>
    <dbReference type="OrthoDB" id="2405412at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000017">
        <property type="entry name" value="Mannan polymerase II complex ANP1 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052086">
        <property type="entry name" value="Mannan_Polymerase_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083:SF6">
        <property type="entry name" value="MANNAN POLYMERASE COMPLEXES SUBUNIT MNN9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083">
        <property type="entry name" value="MANNAN POLYMERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03452">
        <property type="entry name" value="Anp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ82984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="389" mass="45017" checksum="15A5C8FFEC9C3F0E" modified="2017-01-18" version="1">MAIKFNKRILTRYVLPIFSILLIISIVTSRMNSETINGGVSINQHKWAHEGESSFYFPFSKKFRMPAYSYKQKTKNWLYFNAKQKDIIPQAHIAHYDLNQLQSTQDAAVNKEQVLILTPMQTFHQEYWNNLLQLTYPRDLIELGFIIPRSKSGDVAIKLLEKEVKKIQTDKKETRFHKITILRQDSESFNELGEKERHAMAVQKERRSKMALARNELLFSTIGPYTSWVLWMDADIVESPSSLIQDLTQHNKPVLAANIYQRFYNDELNKKDQRPYDFNNWAESSTALELANSLGPDEIILEGYAEMATYRPLMAYFYDPKGPVTATMDLDGVGGGCTLVKAEVHRDGAMFPNFPFYHLIETEGFAKMAKRLGYQITGLPNYLVYHIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R8A6</accession>
    <name>A0A1E5R8A6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycolytic genes transcriptional activator GCR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3291</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R8A6"/>
    <dbReference type="FunCoup" id="A0A1E5R8A6">
        <property type="interactions" value="1094"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R8A6"/>
    <dbReference type="InParanoid" id="A0A1E5R8A6"/>
    <dbReference type="OrthoDB" id="3972204at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060963">
        <property type="term" value="P:positive regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052146">
        <property type="entry name" value="HOT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022210">
        <property type="entry name" value="TF_GCR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37784:SF1">
        <property type="entry name" value="GLYCOLYTIC GENES TRANSCRIPTIONAL ACTIVATOR GCR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37784">
        <property type="entry name" value="PROTEIN MSN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12550">
        <property type="entry name" value="GCR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription activator GCR1-like" evidence="2">
        <location>
            <begin position="812"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="336"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="544"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="626"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="658"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="705"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="383"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="405"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="546"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="573"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="670"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="716"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="747"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="761"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="773"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="790"/>
            <end position="801"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ83101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="981" mass="110045" checksum="7E79CBD45C136F8E" modified="2017-01-18" version="1">MNANGITSSNAGLLSNVPLGNDYENTVKNNDNNEVFSPSSYSTSSNDVEYDLNIFEFFDDAYTIDQPSRLVHMMIMILSKCFQLDNEHDIVSLKLVDLVVDQTYGNRLTLRKLNPSSGSNNYHYINTVQRQTDLSKCPIFGIGLYLLYFYHSSPITIENYNTLPLVKLSSHYINLSMFKYANEKSVQDSTTRFEIKLDTNDTKLEDYSKALFPWLEKLENDFYQKDRTNYALSLLIELFKYICSTIVQDLVYLNTLSDIHNHDLMQIFSFFFDMPIISNLKRNIVSRNRSVKNEIFEIREDIQLLKDMMMKQIELQSKLLETLNNNVSSRANLVNNRRNSATDNKSNNSQNPALLNSSNFQGYGSTTSLSMPQVLTTGNNDNNENRSNDNNNDGHGENEAPFNNEGLNNNAPSNFHNDLPSAAHNNLGNNNINATAMASPMFGNLLQGGTPSLMIPPLHKLSPYVPGSPAPRPFKTTMASPNMFLANLADANSPSLTSSNNIAGSQLQQQQQLNQFQQQQQQQQQQQQQQQQQQQQQQQQQQQQQYSMGASGSTFMSSDVTTSMPDTSTGPVNSNENNNFANRFLPNSLDARISESTGNNMNMNSRYGNSSLRMQLPLSNLISATNKDTSTENTPLGSSQPLVRSPSVTNMLGAISALNTNSLNPPPLTTAMSNTDTVGESLENDGNNIESIKKDASALMSLARLKNGGTNDKSSKTKTKKPEKGKRGRKAKTKAPPQVSIEVTDGMQDTNEQNGDTNLDSSSSSTSNSGFSQNTAANNNNGMIQLNGDTPKKVTKNRDSEFKIRKNHDIKYKLCRENKTIHDLYQEWYHGLDDGKKPSIKSLIQQFGWRRWKVMDDSHFFPTRRIIIDYIERETSLGFSNDKFNINLVSREDARKSVADDLESFRVGNALSLNSISLLLKNMKKNQDFTIYTGSKINLPKNEPKPEPKENSEIIVGQEGYLLRKLTEDEKNNLCKKSIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R8A7</accession>
    <name>A0A1E5R8A7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Autophagy-related protein 21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3328</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat PROPPIN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R8A7"/>
    <dbReference type="FunCoup" id="A0A1E5R8A7">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R8A7"/>
    <dbReference type="InParanoid" id="A0A1E5R8A7"/>
    <dbReference type="OrthoDB" id="1667587at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048720">
        <property type="entry name" value="PROPPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11227">
        <property type="entry name" value="WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21032">
        <property type="entry name" value="PROPPIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="226"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="364"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="235"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="368"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ83137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="471" mass="53085" checksum="BA77A6A98D520216" modified="2017-01-18" version="1">MSIPQQNFRAPLKFNQNGTYVAVTEQDNLSIFNINPFGKIFELQGTSSGLYKSIDLNNASGIMTREELGGVLTGRYLIEMLFATSLIAVVDTIQQPKKLKIINTKRKAAICELKFDHNVQQICLNRKRLVILLQSKQIFIYDISCMQLLHTIDIMEDKVIKELVLKQQQKSWLLKNMAVRGTTSEMDGNQTKEDDAYSYEVKMKLSGDDKSILVYTTYSLSTVDPREDETEITESNENIQKNVNPKGSSASANKLKITLNNIMVFDALNLAQLNFLPRVHNRNVQCLSLSHDGSTVVTASTRGTIIRCFKTSQEQSCFKEFRRGNRPCTITQLEMDHGIALLACVGNSDTIHIFQMNKENAASLADKNTPNNSIPSSSSSPGMALPSTIRHESKFTRVSKFISSNIVSHLPRQNLERNYAYIKKQNEKEIVMIGFSNSSQDKMYVCYGDRLEVYELPSQPGSCLLTQTHVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R8T5</accession>
    <name>A0A1E5R8T5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator MET32</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3239</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R8T5"/>
    <dbReference type="FunCoup" id="A0A1E5R8T5">
        <property type="interactions" value="3597"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R8T5"/>
    <dbReference type="InParanoid" id="A0A1E5R8T5"/>
    <dbReference type="OrthoDB" id="3971415at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="207"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="237"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="17"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="134"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="34"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="51"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="139"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="172"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ82943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="285" mass="32092" checksum="8348397A1383DD05" modified="2017-01-18" version="1">MNFNSFNSTFANLNYSNSPQSQTHTPATAKSPLSAFSTLNDTPNASVRTHSVSASTAAPPSLSSTFYKDNRTTNTLESQNNRKHPFQTEQDMSRFSQEQQFYQQATEALLGIQKNINQVDPVIKELLKRLQNGDPLLDGNTQNIDTSVSNNGLTNTNNNNNNNNNTGLYNGFSSYSNQNRQYSLSNPLRDGHAGDDQETEYPKGTIFECDLCPQKFDDTLDYRKHCKDHFHKNGDLHICPKCFKGFARKDAMKRHTGTKTCERNRNALIEENGGKMPERPPTERP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA21</accession>
    <name>A0A1E5RA21_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative 2-hydroxyacid dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2824</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA21">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA21"/>
    <dbReference type="InParanoid" id="A0A1E5RA21"/>
    <dbReference type="OrthoDB" id="298012at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016618">
        <property type="term" value="F:hydroxypyruvate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF178">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE YGL185C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="3">
        <location>
            <begin position="185"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ83756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="375" mass="42810" checksum="FAA15B48C21E44D1" modified="2017-01-18" version="1">MKKAILFMDTPEYTATYEELKSKFLLVNYSFPKSLTKDQDKQXHFIEFVDTQFDYSXGIELVGIYAGYRAFLTLEDGLTEELIMASPNLQHLQMVTLCSRGINKLPLDFFKSKGVVLTNYDDADNYVANDVADCAMWHVMEGFRKFSFQQHIFKKFDNVLDARCKIRGDAVLDEKFAFGHELSHHNSKALSPMNKKVLVLGFGKIAQTLCLKLKLGLNMDVYYSTRSGESIEGKKQGFKYFPWKQWEADTTNPSETKNNEPSLDMFDCIVNCLPGTKDTLHLIDKNFLQNCCPLNNLILVNVGRGNIFDLHVINDHAKDFRHLGLDVFYKEPLLLEFPQMLYENSTMTPHIGSSTDSCFLGASEYCLKKLLTLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA57</accession>
    <name>A0A1E5RA57_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase</fullName>
            <ecNumber evidence="3 14">2.4.1.258</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Dol-P-Man-dependent alpha(1-3)-mannosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g3015</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 14">Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the first dolichyl beta-D-mannosyl phosphate derived mannose in an alpha-1,3 linkage to Man(5)GlcNAc(2)-PP-dolichol to produce Man(6)GlcNAc(2)-PP-dolichol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29527"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="Rhea" id="RHEA-COMP:19516"/>
            <dbReference type="Rhea" id="RHEA-COMP:19517"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:132515"/>
            <dbReference type="ChEBI" id="CHEBI:132516"/>
            <dbReference type="EC" id="2.4.1.258"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12 14">
            <dbReference type="Rhea" id="RHEA:29528"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the glycosyltransferase ALG3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.258" evidence="3 14"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA57">
        <property type="interactions" value="658"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA57"/>
    <dbReference type="InParanoid" id="A0A1E5RA57"/>
    <dbReference type="OrthoDB" id="20028at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052925">
        <property type="term" value="F:dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007873">
        <property type="entry name" value="Glycosyltransferase_ALG3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12646:SF0">
        <property type="entry name" value="DOL-P-MAN:MAN(5)GLCNAC(2)-PP-DOL ALPHA-1,3-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12646">
        <property type="entry name" value="NOT56 - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05208">
        <property type="entry name" value="ALG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 14 16" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="195"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="339"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="396"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="421"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU364047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ83782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="540" mass="62971" checksum="93AB5A9C22421336" modified="2017-01-18" version="1">MTDSRENFDPSQAAQFQSESQDTKKPTFSKVSKTSNVQVLNNERNKLSVKNVFLDLIESVYSFTFDPELTAGIMIFFVILESLALKLIIRNVAYTEIDYKAYMEQISMIFDEKITDYSQIKGGTGPLVYPAGHVLLFRAMRHITQGMDNIKEGQIVFRYLYLVSMVIDYAIYSMLDNVPPFAAVLFCLSKRLHSIYVLRLFNDCFTTLFVSICCFLLIFCGKHYKTLYKRENSGFIVLLNLIAVLCYSFAISIKMNALLYLPGILIILVWMNYNTKRFLLKMLLYVGVGLQLQISLASLFLAKHSSEYWNCAFNFQRKFMYKWSINWQFVKESVFSSDSFSKTLLFLHAIVLLLFVVKKWIQPVLITNKITLWQFIFKILTLNNELLFPTLNAHHVAYILVSCNYIGVVFSRSLHYQFLSWYHWTLPILFTXAWGNITNKKTMSSSETNKNEQNGINDHQQIIDNVKFMIKSCVWFALHEYCWEVYPPNRVSSALLQXLNLVLLYSVYTNYXYDYTHNYKQVEVADTEKSTEPSLKFKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA71</accession>
    <name>A0A1E5RA71_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein BIG1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2558</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BIG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA71">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA71"/>
    <dbReference type="InParanoid" id="A0A1E5RA71"/>
    <dbReference type="OrthoDB" id="9985059at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006078">
        <property type="term" value="P:(1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037654">
        <property type="entry name" value="Big1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28285">
        <property type="entry name" value="PROTEIN BIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28285:SF1">
        <property type="entry name" value="PROTEIN BIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184665" description="Protein BIG1" evidence="11">
        <location>
            <begin position="21"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="329"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ83809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="356" mass="40227" checksum="04A72ED1CB30BFB2" modified="2017-01-18" version="1">MRXLLSFCLLIATFAQLYTAHEVGLISSYKLSPGLQLLNQGRDILKPISVEKFMSESFSMVKMCQSYSYLFVDLPGVTRSDFIENERSFPQLQKYLELSSSIAKFEKIDYHGENSTKIFEHAIRTTRLSCSVPYKNVLFVEGNNTDSFVPYYDTAPRIIKIEFAEIPAHGTSMSEKRARTKALKEYDTFLGYVLGHLPTPEFSMVLTTSPGTGYQDGVSENANVQQGNTSGIFPSVFNVKSRSKEYERNQRPKKIPKPQFKPYVPLQHSIIERAKIYPTAFNIDFLIENEDILAKILTSLVVSVALYFFVPNPFEKGINLDKKKLRKSVESRKDETVGGHTSPPANPASTNDKKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA76</accession>
    <name>A0A1E5RA76_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">General negative regulator of transcription subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2952</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNOT2/3/5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA76">
        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA76"/>
    <dbReference type="InParanoid" id="A0A1E5RA76"/>
    <dbReference type="OrthoDB" id="25391at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1020">
        <property type="entry name" value="CCR4-NOT complex subunit 2/3/5, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038635">
        <property type="entry name" value="CCR4-NOT_su2/3/5_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040168">
        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007282">
        <property type="entry name" value="NOT2/3/5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04153">
        <property type="entry name" value="NOT2_3_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NOT2/NOT3/NOT5 C-terminal" evidence="5">
        <location>
            <begin position="161"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="22"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="38"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ83794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="297" mass="33734" checksum="925E216DFCB02AD1" modified="2017-01-18" version="1">MGIANYGLETFVDNFLKLQQNSKEPSFEPNXDWNSIEANKDDQRDLKKKDLSEAKNGAKQEKTKKTFDKAKKAHGDDVDGXESAQTQSXEALNSEGNGVSSTAYDDYTMVTGIDLTPYLQSLNIEMPNVVGLNPDGSGDLSASAGYNATXNTRPFHHTLETLASPWIETSRSEVEPRYHSPKSFTNITGVLGTSGEPQFNEVSNQEQNKVSLLQDETLFYLFYKHPTSAVQELTYIELRKRNWRYHKLLKVWLTKDPVMEPIVQPDGLSERGKYIFFDPTKWEKCQRDFILHYTAIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAC2</accession>
    <name>A0A1E5RAC2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">F-actin-capping protein subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2769</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
            <location evidence="1 9">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the F-actin-capping protein beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAC2"/>
    <dbReference type="FunCoup" id="A0A1E5RAC2">
        <property type="interactions" value="891"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAC2"/>
    <dbReference type="InParanoid" id="A0A1E5RAC2"/>
    <dbReference type="OrthoDB" id="9979678at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008290">
        <property type="term" value="C:F-actin capping protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.210">
        <property type="entry name" value="F-actin capping protein, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037282">
        <property type="entry name" value="CapZ_alpha/beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042276">
        <property type="entry name" value="CapZ_alpha/beta_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001698">
        <property type="entry name" value="CAPZB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043175">
        <property type="entry name" value="CAPZB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019771">
        <property type="entry name" value="F-actin_capping_bsu_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10619">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10619:SF0">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT BETA ISOFORMS 1 AND 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01115">
        <property type="entry name" value="F_actin_cap_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00192">
        <property type="entry name" value="FACTINCAPB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90096">
        <property type="entry name" value="Subunits of heterodimeric actin filament capping protein Capz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00231">
        <property type="entry name" value="F_ACTIN_CAPPING_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0117">Actin capping</keyword>
    <keyword evidence="6 9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ83860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="294" mass="33602" checksum="3B4382DE83AA30F3" modified="2017-01-18" version="1">MSDLQYQAALRLFKRLDPSKIPENLISLISLEPALSDDLLNDIDQPLKVKSATKGKDFLCCDYNRDADNHRSPWDNTYYTAEGTALSETESDEEMYYPSEELRLYEKLFNDSFEVYKDMYYEQGSYSSCYLWETEESLKEGFAGCVLFKKSLTTGDGGNWDSIHVFEVLQNTDSDIQAYEYRITSTIILDLQQKASSTSKIGGNLQRQAEKTVSLDVADGFEFNSSHLDAFHLTNLGNIVEDMENKLRTMLEVVYFDKTMNIYRNLVKDSSLHGSSGDYHRDLQENIAKGISNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAM4</accession>
    <name>A0A1E5RAM4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cullin-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2589</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2 3">Belongs to the cullin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAM4">
        <property type="interactions" value="815"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAM4"/>
    <dbReference type="InParanoid" id="A0A1E5RAM4"/>
    <dbReference type="OrthoDB" id="27073at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cullin family profile" evidence="4">
        <location>
            <begin position="393"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ83959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="681" mass="79704" checksum="ADE4E69973DB6576" modified="2017-01-18" version="1">MVGNKKVRIKAPRKMTWIQDGQTPQQFFDTXWPEVSEAVLLILGDIESSAQLSYQELYSKVYQICISQMSKTAYQELGNTVSXYLEQCSENSNFESLESFHKFWSDFVEKVKIVTSVFLYLDRVYSTGISIPQCYDMCLLKFRDHAVLKHIEFLKEDIGVCVKSIRDFSFENTAPKDLNETQISTNSKKLVMXVFEMLQLLDLFGNTTVFHHHVEPYLLQESKTYYEKLADCNLQSMSSSEYIESIYKIFDTEKLVNSEITTDMDYHIKMEKVIYXLFITQKREHFVSNCFKNFINEKHYQDIVKIMDLFSKEEENMNYLMXVFAKDCFQSFSEIQEDSALPRKKQQTKWIQDFLDISKEYNVLIEHLSSLKTQMLTHVNEGISQAINRQSNKSAEYLSQYFDFFLRSETKWNENMSSFNLFDKTVVRCIRLLQLIKEQDVFELFYKQQLSKRLLQRRSNLEAEKHVLIKMKDALGSNFTAKLEGMVHDIVSSLADNKKFNNSPNYAHPFELEASILTNTCWPFQSTQTFTDEEKTLALPKQLMKYGLDYENCYIKTHEGRKITWAHPYGFLDIGYQFNKTYHVINMPFYAGLIFLLFEENQQLTTREIKDLTSIPSNEVVRQLASMALAPKTRILKKSPMTKSVKDSDIFSINEEFTTETXSVKLVLAIISKSSNKEMQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAM9</accession>
    <name>A0A1E5RAM9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Mitochondrial phosphate carrier protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g2749</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAM9"/>
    <dbReference type="FunCoup" id="A0A1E5RAM9">
        <property type="interactions" value="795"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAM9"/>
    <dbReference type="InParanoid" id="A0A1E5RAM9"/>
    <dbReference type="OrthoDB" id="427452at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990547">
        <property type="term" value="P:mitochondrial phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000131">
        <property type="entry name" value="Mitochondrial phosphate carrier protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044677">
        <property type="entry name" value="SLC25A3/Pic2/Mir1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 (MITOCHONDRIAL CARRIER PHOSPHATE CARRIER), MEMBER 3, LIKE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671:SF10">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="12"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="109"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="208"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ83956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="301" mass="33642" checksum="E1B399CD19787666" modified="2017-01-18" version="1">MSSHKIELYSPEYFKYSFIGGILACAPTHGAVTAIDTIKCRLQVDSHLYKSNLDGFKKIISQDGFGALYTGGAATFLGYGLQGFGKYGFYELFKYKYGEFLGKENAEKYRTSLYLASSASAEFIADILLCPFEAVKVRQQTTLDFKQTKSFVATWKEIAQHGGLYSGLGPLWFRQIPYTMVKFTTFEKIVEYIYDYFHLQKNKMNKFQQASVSFAGGFIAGVMCAIVSNPADVMVSKINHNKLASESTSQAVKREYAKIGFKGLWSGLPMRIVMISSLTSFQWLIFDSFKISVGLPSTGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAQ3</accession>
    <name>A0A1E5RAQ3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Regulator of phospholipase D SRF1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2906</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAQ3"/>
    <dbReference type="FunCoup" id="A0A1E5RAQ3">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RAQ3"/>
    <dbReference type="OrthoDB" id="2589563at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037737">
        <property type="entry name" value="Srf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36819">
        <property type="entry name" value="REGULATOR OF PHOSPHOLIPASE D SRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36819:SF1">
        <property type="entry name" value="REGULATOR OF PHOSPHOLIPASE D SRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="326"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="368"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="405"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="457"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ83984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="496" mass="55295" checksum="430818D22FDE9C41" modified="2017-01-18" version="1">MAPNKNLEALESRDESENSSISQSHESTEVPTSNTKSSNTSGSDTKNKVDSENVSNTNNNHASTPALNSEVWLNSYSLNATTIPPFAVTAELTRERQESEHDPVAESQYLDQSREKAVITHTGGALNYSTAKDSLANTSTDPFLNSQNSVWGSFMTNVGSHTSYARNQTRALPLERKPNQVPEFEEKYTTPPESADESSFVNDLDSGSFRLNQFSKDEAGSTLDAQSIFADLNGSWGGQERLEAVLIAPGLKDQKFKNSKDKQEFQKNLKELRKVYYSSEKKTVGVKQANNKEKVATYFKQQYGVKKRRLIPQIKRTLFDNRYLPLFFRVSTIALCIIALGLASRIFINCKGNYPETDITIHQQPSTIMALVVNSIAIVYVVYIATDEFKGEPLGLRDPNGKLKIILLDLLFLLFCSANLALAFNSMYDAQWACHAGTDLTNRSPKLNYVCRKQKTLSAFLFIILCVWVLTFIIGIVRVVNKVGKFSQRGRVANSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAQ4</accession>
    <name>A0A1E5RAQ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Purine-cytosine permease FCY2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2740</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAQ4"/>
    <dbReference type="FunCoup" id="A0A1E5RAQ4">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAQ4"/>
    <dbReference type="InParanoid" id="A0A1E5RAQ4"/>
    <dbReference type="OrthoDB" id="2116389at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015856">
        <property type="term" value="P:cytosine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11484">
        <property type="entry name" value="SLC-NCS1sbd_CobB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4160.10:FF:000002">
        <property type="entry name" value="Purine-cytosine permease fcyB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026030">
        <property type="entry name" value="Pur-cyt_permease_Fcy2/21/22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31806">
        <property type="entry name" value="PURINE-CYTOSINE PERMEASE FCY2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31806:SF1">
        <property type="entry name" value="PURINE-CYTOSINE PERMEASE FCY2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002744">
        <property type="entry name" value="Pur-cyt_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="167"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="239"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="308"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="354"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="408"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="438"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="478"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="517"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR002744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ83951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="543" mass="59324" checksum="68E16C7FBE3F41A1" modified="2017-01-18" version="1">MASSSGVENYELNQTTQYQNDDLEKKNHVYDDIIEEDDSSYTGSASKFKYGDETAELSSSSKFTSKSPFLMKLVKLMDLAETKGIQPVPEEEKTDSSLYDAGTMWLSANLVVATFALGTLGGLVFGINFGACVLTIIFFSLLGCVPVAFFSTFGAHTGMRQMVLSRFLMGNVCARVFALINCVACVGWGAVNTIASAQLLVEVNSPHNCPPWAAVLIICIGTWFITFFGYKVIHFYEKWSWVPTFAVFLVIIACLRKKDTFENGPWGGGPTTAGGVLSFGSTIFGFAIGWVSYISDYTAYMPRSTNKIALFFSILCGSMIPLCFVLILGAAAAATTLVTPQYMTAYTDRGVGGMLYAIMVEDSLHGFGQFCCVVLAMSTVANNVPNMYSIAFGAQSIWSPLKKCPRPLWTTVGTGITIAIGIGAYYKFTSFMSNFMDAISYYLGIYTTMGLSEHFIYNKGRMSSYNPADWDNWHKLPIGIAGCCGLFVGAIGVAVGMDQTYWVGELGRKIGEYGGDIGFELGVGFSFIVFNLLRPFEKKYFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAT4</accession>
    <name>A0A1E5RAT4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">60S acidic ribosomal protein P1-beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2910</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes). The 5 acidic ribosomal P-proteins form the stalk structure of the 60S subunit. They are organized as a pentameric complex in which uL10/P0 interacts with 2 heterodimers, P1A-P2B and P1B-P2A.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAT4"/>
    <dbReference type="FunCoup" id="A0A1E5RAT4">
        <property type="interactions" value="723"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAT4"/>
    <dbReference type="InParanoid" id="A0A1E5RAT4"/>
    <dbReference type="OrthoDB" id="2194681at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05831">
        <property type="entry name" value="Ribosomal_P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696:SF33">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="64"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="85"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00063629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ84016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="107" mass="10660" checksum="925E561A9A68914C" modified="2017-01-18" version="1">MSSSAVVSFASLILADAGLDITSDNLLSLSKAAGAQIDSVWAEIYAKALEGKDLKEILSGFHAAGSSSAPAAGAASGSAASGEAAAEEEAEEEAEESDEDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB41</accession>
    <name>A0A1E5RB41_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DNA-directed RNA polymerase III subunit RPC4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2933</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB41">
        <property type="interactions" value="169"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB41"/>
    <dbReference type="InParanoid" id="A0A1E5RB41"/>
    <dbReference type="OrthoDB" id="5836119at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007811">
        <property type="entry name" value="RPC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13408">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13408:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05132">
        <property type="entry name" value="RNA_pol_Rpc4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="24"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="50"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="63"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="229"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="252"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="6">
        <location>
            <position position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ84114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="500" mass="54864" checksum="D39646BD83033E67" modified="2017-01-18" version="1">MVGRLASLSSSGNGSSGGAKKPTGTGNDQNNNNGSLGQANGKDSAPKAKPGLKFKPKVVVRKSKAEREAEQLKNQQKLEEEKLKAARHNEKNRYSKKLTNNGKRVPRYLQNTTVVSSGPLAAGNFVSGGSGGPKGVSFGSSFSSGSTSGGSVISELMGNSKLTASILESKDGDDDDDSDSDTESRFKGKKHGTKEEGADEDAPIKSSNKRDGIIRINMSKEFKIHEILSDDENDDEDDDESGNEXNDLSITEEGKIKQELGKDGDGDVEMKNGEENDEGKDXGKKQDLSYLFPVRPIRVKHEDVELAKQELQDSYSLPNTREPTPGMVKQEDAEDXNQXGLRGVENQERALLRKRQLDLEQKVKAMHLDKDFHSVDEAESLEEKKMIVKDYLYLKRKLNKIHNQDRQFFALQLPPQLPFDNADEQNALQGQPNIQGNIGQLRVHKSGRITIKIGKVVMDVAKGSETSFIQDVVAVDTAEDENTVEHLGQIAGKMVVTPRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBF2</accession>
    <name>A0A1E5RBF2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mitochondrial intermembrane space cysteine motif-containing protein MIX23</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3055</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MIX23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBF2">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RBF2"/>
    <dbReference type="OrthoDB" id="3971669at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019171">
        <property type="entry name" value="MIX23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31905">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31905:SF2">
        <property type="entry name" value="PROTEIN MIX23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09774">
        <property type="entry name" value="MIX23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="212" mass="25206" checksum="7FA7B1131A1CAE56" modified="2017-01-18" version="1">MKTPQTFEHSKLXINNPKSNNNERNALIIQNPHYLSGDNIPESIRFRNLELTREHCVDMSLLTSYFRFLRHYSDDTLQSRLNDSEKPDMSSKLRRCETYTGKLFGNWEIRRNIMTFCSKEAETLKEELDSTFEQDNKKAVTAAKIDSYALRDLQNEKNRYYANYNKIIQWVSQQEGVEKILQDRANDVLRNNCDENKNYLSDFKLFYKDHIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBF9</accession>
    <name>A0A1E5RBF9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">U1 small nuclear ribonucleoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2855</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBF9"/>
    <dbReference type="FunCoup" id="A0A1E5RBF9">
        <property type="interactions" value="441"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBF9"/>
    <dbReference type="InParanoid" id="A0A1E5RBF9"/>
    <dbReference type="OrthoDB" id="4207594at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21615">
        <property type="entry name" value="RRM_SNP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051183">
        <property type="entry name" value="U1_U11-U12_snRNP_70-35kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022023">
        <property type="entry name" value="U1snRNP70_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN 70 KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952:SF5">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN 70 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12220">
        <property type="entry name" value="U1snRNP70_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="119"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ84234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="250" mass="29355" checksum="BF01A31482A84E20" modified="2017-01-18" version="1">MSSKEDERMHLIARPRRLPEVKQSIDYPKERRCTNPYISGVAQLLQNQSAHITHLNETAKQEESLTKVTVQPKEHTQYTQNAIRVENHNYQSNLSDYQRQLREWNPHEDIYSKSTDPYKTLFVGRLPYDLGEVELSKHFTKFGEIANFRLVKDVKTGKSRGYGFITYKSEISLRKAFDEVGESGVLIINDRPVIVDFERGRTVHYFKPRRLGGGLGGRGYLNEYKQQKRFAKMKKNQNIQAQRRSNREYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBL4</accession>
    <name>A0A1E5RBL4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DASH complex subunit DAD2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Outer kinetochore protein DAD2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g2515</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex DAD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBL4">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RBL4"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013963">
        <property type="entry name" value="DASH_Dad2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28036">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28036:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08654">
        <property type="entry name" value="DASH_Dad2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="16" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="unsure residue" description="D or N" evidence="18">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00030568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ84271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="125" mass="14333" checksum="882AE002F627A3F2" modified="2017-01-18" version="1">MDRLIEEKQIELSLVKKLGLQTTEMVTLLNKLVQELEAVNSDVEKVDKIMSNWGFIMKCISASSISLLQYQEQDYEKGAWTXQIKNSHNKETQQIIPASEDQERQTKVPLPEVMVRLGLNDSLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBV1</accession>
    <name>A0A1E5RBV1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g2977</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:47005"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBV1">
        <property type="interactions" value="1447"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBV1"/>
    <dbReference type="InParanoid" id="A0A1E5RBV1"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF565">
        <property type="entry name" value="AT28366P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="12">
        <location>
            <begin position="23"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="98"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="410"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="537"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="421"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="539"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ84369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="561" mass="62785" checksum="1E81CC010CFE5458" modified="2017-01-18" version="1">MGQILSNPDIRKEXEKMNDKFTAYGVCSMQGWRMSMEDQHITQLSLKPSAQDALQNFNDQLAFYAVFDGHGGSSIAEFSGNNVNLILSRELNSLYKKQNNDKKNDSKGDSSVNNNNNDNNHMNNSNTNNDRDQYAQAMIDCFLHTDEEILKDKILKMDHSGCTATSVLISKQYGKIVCANSGDSRTVLSSGKYAKNLSFDHKPTNAGEKARIFNAGGFVEMSRVNGNLALSRAIGDFEFKQNNDLPPHEQIVTVVPDILEHKIDYTNDEFVILACDGIWDCLTSQECVDLIHYGIHKENILDLGELSSKIIDVCCSPDAGGQGIGCDNMSMMIVALLKNEESVDEWFTRMKSKSYDLDKEINFETYRKKIFNYFQFNNENIFAVTNPATYSNSNNAGGMNRMGGLSNGGLGSLIRPRMNYGDEEDDDEEXXXEEEEDPDYEEDMGAHHAVTLKTVDKKHAKKGTNNSADDQVEVAIDEEPMDEDDENNSXGNSDSKQIVLNLDDFLKNNGSLTRDENGMTYIQGHILSEVLESLVHKRQGTDKAVESDEQVKNDSEDDKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDK9</accession>
    <name>A0A1E5RDK9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2058</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RDK9"/>
    <dbReference type="OrthoDB" id="4061472at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="549"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="122"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="154"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="549"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="687" mass="78465" checksum="B1F2772ABDFAC5C5" modified="2017-01-18" version="1">MSVIAYENELDRHRGVIPSIATTLKHEKSKMAKIFKRNSFCNGNNNYGNNTMMPQGMNQKTPIIEKILYNPFNLQDNPYLPHYKDNHNGLDIELKIMNPNKQIIFPSITKLNKRSVFTNTTSNRGDSSGSVNSGVNSNDDNNTNTNFDNEEINNNDNGQDQDQNQNQNQNQNQNQNQNQHQHHFNKQNSIKLKKNGKNAEIYGEIVIHTPKRNTQNNGLFVIKNFKIKICGYILNFKCQVDPRTKXMIIIPIVNSKERKRVHVPFIENDIVEYVAITTNGKTSTTSTLTDKYKCASTSMPLVLDQSDCNYIFPFKHLINSNDFPSSLYSTMGKIEYRIECYLTVENIGNILLTDKMDIHKSIEPASGCMTNDNGTLTRKGHVYNFALVTSTTTTATTKRTTNNKPILTSEQIXEIKYNFYLSSRVIVLNQLFDFTIDILNDKSQHHFKITHVSVELFQVFKFPNDKLKCKSYSLYSCESTKYSXFPTTYSHSLDSIKVSNVRISNEMVNKNYQSNGNKDYGDYYISSQSKVSAGKSDNSLTCITSAKSNTSNNSKVGSKLSPKLSPKLSPKLSPKWSPMSKDFKETSPPRCQLRSPYDDGLIPYYNEKKNVRKRAMLNEFISIGHFLKFTVDIEDNTTLTNEKYKLSNGKKKLKEFTKIKKTMTFQIPIVLVSELMMSTMTLPEYVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDL9</accession>
    <name>A0A1E5RDL9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Meiotic activator RIM4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2308</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDL9"/>
    <dbReference type="InParanoid" id="A0A1E5RDL9"/>
    <dbReference type="OrthoDB" id="410044at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017069">
        <property type="term" value="F:snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051183">
        <property type="entry name" value="U1_U11-U12_snRNP_70-35kDa"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN 70 KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952:SF6">
        <property type="entry name" value="U11_U12 SMALL NUCLEAR RIBONUCLEOPROTEIN 35 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="50"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="276"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="403"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="525"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="11"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="552"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ84989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="677" mass="77554" checksum="4A99BC032B8D0F06" modified="2017-01-18" version="1">MKAYSQNTESNCKDHSFIRSEGENDKTKVNPRSSSLEANFQSQTRSRPSCCIFVASLPSYLSDDALWVSVVEHFEKFGDVAMAKVLRDNKKRPYAFVQYHTEHEANAALLKANNSMLQNRRIRCEKARVNRTLVIEEKFPVSNSEKAVLSRLEKIGEIEKMVEVKQYYAFKGLLNNHNHYQLTSNPCRSWYFQFAYRDDAISAHARIRNEGVYTAEWTDNIEVPDTLNVVQHLQECLKEDKKVYGYEAQDIQSSQPEKNNNVQIYNSSSGNEIDTFSIFIGQLPSGCTEEELAKKFGTHGEIESINLFTKQENNSFAFIQYKNEKSASFAIENENHSFFKSKTIHVQFRERNHVNAEKKRHMSYFQNNSFGMDLPNPIMPNAVSYARNPNESFDHKQRTTFNKQTKFSSSMRXPDDXRVFPNTPTNRADXYQDRSFKQVDYKRNKFEKSGLPNRNFKGTGGTHEDANLIDKNQQNTLPPYHLQPNFVNTNGPNYXHFVPPYYYNPNVVYYPNQXYSKENQTQQDYDFHDDSSVSKFSSSGENPTHLPEKENGSYSQIASTLPETFGTSSSEPNENTYASSGAPQRMPQPMLNSLGLNQHSIHRNYDVERHNHPPNFGNPAIMLPFAGNXAPGMFPVATQPPLPSQVLPFAYGAANPEFHSXAFEHGNVYKGNFHKXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDQ8</accession>
    <name>A0A1E5RDQ8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Proteasome component ECM29</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2318</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDQ8">
        <property type="interactions" value="1023"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDQ8"/>
    <dbReference type="InParanoid" id="A0A1E5RDQ8"/>
    <dbReference type="OrthoDB" id="16066at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024372">
        <property type="entry name" value="Ecm29_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055443">
        <property type="entry name" value="HEAT_ECM29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346:SF19">
        <property type="entry name" value="PROTEASOME ADAPTER AND SCAFFOLD PROTEIN ECM29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR GCN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13001">
        <property type="entry name" value="ECM29_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24492">
        <property type="entry name" value="HEAT_ECM29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Proteasome component Ecm29 N-terminal" evidence="5">
        <location>
            <begin position="15"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome adapter and scaffold protein ECM29 HEAT-repeat" evidence="6">
        <location>
            <begin position="1296"/>
            <end position="1458"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="7">
        <location>
            <position position="1053"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1904" mass="215049" checksum="906B582A2150EE36" modified="2017-01-18" version="1">MSTPTDQEKNELALIDKVELRLALADDAAKYTQFLDTFLCPLLLKLASPHSTVRAAIFNLVKDITSRLNSYPEIKLPVLKLLKQAKNPNTQSTQDSQSVSLYSLLLASKGVTNLQIACKDEILLECCNGISELPSAIAARMFLVLLKLVAKWPGPKLGTLEETNTKDFFSLSANDTNFLSSKFIKFFLLIPAKPVASGQPIPRGYVCPGLSLQDVAFFTFDAGVTYTKDQLKAFRKSLFRFITQGINFGDIELVKNLSVISVDSSNNAEQAQTLLKRMQIPYENENFVSFLIELYTGNKQTGTPPVPNPVKEVILNVLLRSNLATKTPFVKTICSIGLNAEEYKIRSKTLKYIQHVAKYNYKALLXEDADSDTDSETFHLDITSLIRSNLHTEGWPRIQIGQGTPNIHLSIEQRRTEYETLGELLQHTKLEDLSYIEFLIQSLLKDLPDFRTTIQNVLTGLALNLYQNLKEEPKTQLKNIMKQYLSDDTEYLQMNKDEKDSFMSLRFVCLRFCNTVYSFDDCDARLLDVFGFSKKNRFDVMEEAMKGLNPYWYKMNKSFLNKSFKDSLSVSTEEIKFPDFQSFVQLLLNEXKICLTYKNHALRESLLAGIRFALRTAISQAVFQKQTIIQQDQDWSLRVEQCLSIDFAVEKLFKNFLLXQEGEWYIGFLKLLLTEFCGQNNNEHISASSNVVMGDILHTFVKYSKADVLKQLEXFQQSLCFLVGNQSTKLHDLDNAVEIFGMISCQLDVQNPVTQLLLEKIEPSISEREFAPIFRTACSVIARFAAKSEGSVFSVQINKIYDIIEAKLKTGKAIYRNDALKCLTHLSKYGCFEFLNDKSAYHNIANILATNVMNDEAGLVAFSTMLIEHDGDIEKPFERVIMPTHQNKNVDFLFTVGESLTIIMGKWESVFLARQLDVSGVDIKXLEKKYSRNIQFGTVLKRILHLSKTATSPNMIKALCIWLLSIVQYCGSFEEVKNSVNEIHSVFMRFLSGRDEIIQEVASKGLSITFELGNADFKEDMVKQFFKSFTNSAASLGNNNNVGSDTELFEKGDLNTGDGSVSTYKDVMALATEVGDPSLVYKFMSMAKSSSLWSSKKGLAFGLGAIVSKTSLSNMLLQNGDLAKKLIPKLFRYRFDPNEQVSVSMNHMWKVLVPNSSEIVEEFFDLIMQELLTSIGDREWRVREASTAALRDLLQLFPQSKYEKNLEQIWTXAFRTMDDIKDSVRQEGLRLTRVISNILVKSIDKNKSSVESEKVLDILLPFLMGPKGLNSDAEEVRDFSLQTVITLVKDCNPQGIQKYIPSLVEDLCLLLSSLEPQMVNYVALNADKYDINMKLLDAKRAHQANESPIMDTIEKLIDMAETGSYDQLFDAVVTTVKKAVGLPSKVGAAKLIKLCCTKAPVEISASSGKFLKVCLNNLKNSNNEMITSAYAVAFGYLFKNSALNKMIKYSEKIVALYFESSSSSNNNLNNKLASGVAIQAIVNYSPSQFEQVKGIFIPFVFVSKNFAQSFLRDDETEEVSKTIKRINGVFQHIWTESTSNSEADTIQYYLNEIIKINAQHIKSSDFKVRVMCLKSLAELVSKVKVSQTKENNVSQIFDILTQGVEGRVWDGKEIVISTLIQACIKFQTFYNDHKNVKHVVETVLSVELLKKTRKYAYSVVNSFCDYCVNFPSQWATELLICGSVPLGLVEKEGEHFQIGDGFLDADGDAAMSDINMKSSQKNIDKETERNNLLEHLSRACCWYVVTDSLTAEEEEKKFSLKLFNFIISNVFYAMTLKNPIVYTWRTELMSCKVGTILVNNFFSTTGSTYNNQQKSKECLETMKSFEQYWKLLYQCIVVDMRNKSIENVQIQLVRLGGLLLQKYPDSTQSIHAVVKDDLGRLLEASSTSSVVKAELKVASVSVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDT1</accession>
    <name>A0A1E5RDT1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Pre-rRNA-processing protein FHL1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2267</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDT1"/>
    <dbReference type="InParanoid" id="A0A1E5RDT1"/>
    <dbReference type="OrthoDB" id="5954824at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060962">
        <property type="term" value="P:regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00059">
        <property type="entry name" value="FH_FOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22701">
        <property type="entry name" value="FHA_FKH1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045178">
        <property type="entry name" value="Fhl1/FHA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21712">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN FHL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21712:SF29">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN FHL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="5">
        <location>
            <begin position="505"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="Fork-head" evidence="6">
        <location>
            <begin position="774"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="3">
        <location>
            <begin position="774"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="199"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="618"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="862"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1019"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1139"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1176"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1200"/>
            <end position="1512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="227"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="253"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="625"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="741"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1034"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1218"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1240"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1296"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1308"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1320"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1335"/>
            <end position="1349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1350"/>
            <end position="1402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1403"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1414"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1427"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1442"/>
            <end position="1458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1468"/>
            <end position="1484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1485"/>
            <end position="1497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1512" mass="161956" checksum="5F400AD7A36491AE" modified="2017-01-18" version="1">MSLNDQNSTDKNERDQDQNATFGQKHAIIDKDPETQIESFKTLQTHEKPFSSNNTLVKSDSALDVEHVMSATSIPDLVPRKRLDSTSLLLGLGTTDFDGMDDGFKGPRLSMSIFEEDPRTAFRDSLKPSLNSSTEFGXGSRKDYNVFLEDDLGEPVQTTSGEAXDKEFAKPDYEMDEQAQQLLTSSLQDDFITKAHKNFDNDENDEKTNSHQLSSQQFNNFSKNENKEEEDGDDDNEIVQFDIPEPHEKSRHSSSQFSQRKSSFSGLTLGGRKASLSSEGVIESLLGLDGGKDDFLFGSEEQKLQRRLSRSGSGVRHRLGLNALRDLKHASQENAISDGEGDSKAKEDCVKEKPLDLVLHSNPTTPAAKTALKXKSNSVPAKKESFDRTPQSILMAKNSMAPLTKKSSSTSPISNKQDMLQKTTSSLQEIEDNSKNSFSTKANEEKDLANNIANLSIKKETEQNSAQNSAIFLNSTDITSSTSGISAYARLDFQDYTFFVQTLQVTIGRRSANDPFVGIEEHKVDVNLGSSKNISRRHAQIFYNFGNDRFELTVLGKNGAFVNDQFIGKNVTVGLKHESKIQIADIPFVFVLPENMNDPIHENKFKTKAELDKAKAEAKNKKKAAAAAASKAKTQVRAKANATKPPAKKKSTPNGSKPALTKTGVEKAKPGRKPKSAIPVEKTPVKGSPDIIEIPGPSLEEPEXLSNSPQDLAPAPGKKVQSPEPKKAATPKKTTKSPAPKKTPTPKKPPTPKPTREPKKVYTLDEIPVEYRTKPTISYSTMILECLRKYCSPKGMSLADIYASIRTLYPYYKYCRESWQSSVRHNLSSNKSFVKVSKQGKGWLWGLDEGYVKEREEAHQKLEDEQKIREQKEKEEQQKFFQRTPREPKKSETLEEANKRTMHYLQEQLIILTRDRKGLDRQTTTNILTQALAMTINQVSQVAKSKGLKGNPLTTLIDRNPQQLNLILAAAVNACTNKITNGRIKQLVQLPSAEKLKEQALIAAKEEEERKAKELLQNANHSQVSVPSLAKSKTAPSATEVSDVSSPDIEEISVTRVPLKNGKGKLASGSMKHLTYEHTSVANGVLQSSSKFPSAAKNVKVSQKAEKLGVTGKDTPAPRFQLMTPATINSKGTSMRITKPKTKTKTSNSTFKGGLNSSPSLKEAVSSSGGFSGVISLSDVSSKTGKKKVSKKEAMYESPDIEIISAIRETPKSESAVKSKSVSKKSTAASKQMKAPPTFDPTSLSKFFQPKQPVSKSKTTSPVPTLVPSKPKERPVGKVGRPRKNQSEPAVKISSPKLSNVSSVANRNSKKTAIPKKRSKQESETSSSDDHNESGSESGSESGSDSGNDSGSGSGSDSGSESGXGSGSDSGSESGSGSGSGSGSGSGSGSDSGSGSGSGSGSGSDNESRSGSESGSGSGSGSGSGSGSDSESGSRSESGSESGSGSGSGSGSGSGSDSGSGSDSGSESGSGSGSGGGSGSGGESQSGSDSESGSGSSDGEEKDAVITKKNTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDW5</accession>
    <name>A0A1E5RDW5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CBK1 kinase activator protein MOB2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2369</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDW5"/>
    <dbReference type="STRING" id="56408.A0A1E5RDW5"/>
    <dbReference type="InParanoid" id="A0A1E5RDW5"/>
    <dbReference type="OrthoDB" id="10261121at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.30">
        <property type="entry name" value="MOB kinase activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021463">
        <property type="entry name" value="Methyltransf_34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005301">
        <property type="entry name" value="MOB_kinase_act_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036703">
        <property type="entry name" value="MOB_kinase_act_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22599">
        <property type="entry name" value="MPS ONE BINDER KINASE ACTIVATOR-LIKE MOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11312">
        <property type="entry name" value="Methyltransf_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03637">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01388">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101152">
        <property type="entry name" value="Mob1/phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="341"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="434"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="341"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="594"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="599"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR605301-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="651" mass="75930" checksum="4D7776D21AC23DAF" modified="2017-01-18" version="1">MSKQLPTESEFALPPQEILDLYKFTFENELYNKNEAIFDELLEDIQEVKGELYDRNYECAFDDEFKRVAYCIRWSPSRSIAYSNLFSHIEPVRDILQNDDSEQTQKVLCIGGGAGGEYVAMASIFTLSRDFNKLYLKKEKDFDRQEKDDEKREPLGNDTTKLNVQLIDFADWDKVIKRVSTQVEDLWLRKLSPELKVDFLHKDVLKDDLSNLPDLNLITLLFTTNELFSENKSASIRFFQKLNAQCSAGCYLLIVESAGSFSHIQVGTKKFPIQFLIDTLLLGKNKKDGEWDLIDENDSLWYRCGKDLDYPLKIENMRNNTSNNSNNGTPQSSVYKQSIVPGNSIYSNPQHSSSRISLKTKASKKQQQQQQQLQLQQQQQQQQKQQLQQQQQQQQQQQQQQQLQQQQQLHQQQLHQEQLKQQQVQQQQEQQQRQQTQQQIARGSAAPQNHKQPTETLQSMFLSEPFVRTALVKGSFKTIVQLPKYVDLKEWVALNVFEFYTNLNQFYGAIAEYVTTDKYPTMNAGPFTDYLWLDANNRQVSLPANQYIDLALSWISNKVSDKNLFPTQGGATFPGNFPRDVQRIMIQLFRIFAHIYHHHFDKIVHLSLEAHWNSFFAHFISFCNEYSIIDKKEMYPLLPLIESFEVQGKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REU3</accession>
    <name>A0A1E5REU3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Endoplasmic reticulum lectin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Protein OS-9 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2124</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Lectin involved in the quality control of the secretory pathway. As a member of the endoplasmic reticulum-associated degradation lumenal (ERAD-L) surveillance system, targets misfolded endoplasmic reticulum lumenal glycoproteins for degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Peripheral membrane protein</topology>
            <orientation evidence="1 7">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the OS-9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REU3">
        <property type="interactions" value="85"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REU3"/>
    <dbReference type="InParanoid" id="A0A1E5REU3"/>
    <dbReference type="OrthoDB" id="3972855at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045149">
        <property type="entry name" value="OS-9-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15414:SF0">
        <property type="entry name" value="ENDOPLASMIC RETICULUM LECTIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15414">
        <property type="entry name" value="OS-9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 7" id="KW-0430">Lectin</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184739" description="Endoplasmic reticulum lectin" evidence="9">
        <location>
            <begin position="22"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="10">
        <location>
            <begin position="142"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="439"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="478"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="487"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU369099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="546" mass="61136" checksum="1950AEE7786911C5" modified="2017-01-18" version="1">MNCFWKQFHVVLLTCISVANALVFQPLIEDPFDVEKYEMIIYDNAESLDRLMSFVHGAGYQIENRGSIGTYTDCIAATPVSNAALDSNGTDEEDKLSSEKIEVILEQAVNVLNQSFFTSHPSSNFQQGQGLQNKAPSFPPTSDAKFIKYVLSRDGFWTYKFTYSQDIVQYAGETLNYTLGVHNPSLSPQLLSNEDGFYISESYDMGMICEPTNKPRTVEVQYVCDPDIAAAQQSPFRAPGVKLLWVKEWKICQYQAKVAVSGLCKIPLLSKATESHSSTKMFCKDPFRIDSTKDFKKSTKRNTAMSVITSPNYETHFVGNNYYFLSNKDEEETDYIVNSLSVADFTMDLAFSSLLGPLGPAILTSLVKLPSDVVATKRDFQWMCEIFGLNGDYLFTAGVNFGSQSSVEFHQKRIQNTRGIMHGNFIFLSDLKNESFQNGLNDSKSQTNENFASDSASNKETVLQKEEITGANENERLIESNAVSDTNHFDESTAARAEKQETSSSXDSTEEPEKPIPYLGDVESKEENENVIVFEKTQQDQESEAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REY1</accession>
    <name>A0A1E5REY1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Riboflavin transporter MCH5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2094</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REY1">
        <property type="interactions" value="177"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5REY1"/>
    <dbReference type="OrthoDB" id="6509908at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032218">
        <property type="term" value="P:riboflavin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17352">
        <property type="entry name" value="MFS_MCT_SLC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF177">
        <property type="entry name" value="RIBOFLAVIN TRANSPORTER MCH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="284"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="320"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="445"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="4">
        <location>
            <begin position="78"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="477" mass="53082" checksum="D69039EBA6BE884D" modified="2017-01-18" version="1">MDNNTKAEDQNIQNTELQRLSQDGIPQQEYTPNQKNQLGITDSCNENTLFAGKSNGLEMNDFLDDEIFYPEGGLRAYLVVAGCFFGLIPVFGTPNTMSVLETYIAENQLANNNASSIAWIFSIFSFLMCSSSILSGFYFDFKGCRDLMIIGTLLHFASLMLVSICTKLWHFILCFSIVNGVAAGILMSPLVSCPSHYFNKKRGNAIAISTIGGSLGGIIYPLILKPFFNTHQYKWGVRTWAFIDLFLLVLSACLVKERFHAVDQDFRTEIKQAVDFKSFLNKNYLFCVIGVVLAEMSITSSLTFFGSYCTKVGISLNDSYNMITVINVLGIPGRWIPGYXGDKIGRYNMAIATLTILSIISLVGWLPFGNQGLXAMYVISAFYGFSSGSIFSLLPVCCGQISKTKDFGKRYSTMYFVVAFGTLVAIPISGAIIGPDKKYHGYQSFIIFCSVLSVFSVFSFYISRYYSIKTFKLNVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF27</accession>
    <name>A0A1E5RF27_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Meiotically up-regulated protein Msb1/Mug8 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1728</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RF27"/>
    <dbReference type="OrthoDB" id="3362494at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012965">
        <property type="entry name" value="Msb1/Mug8_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08101">
        <property type="entry name" value="Msb1-Mug8_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Meiotically up-regulated protein Msb1/Mug8" evidence="1">
        <location>
            <begin position="193"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="722" mass="83349" checksum="A11F7E6156A1B999" modified="2017-01-18" version="1">MKSFAKDFKSKLTHTNFNCFSSKNAPQTTHTETNXDFNLKRXEIKRKKTFIKKSKLVISAXTDFENGVAISDXQNAEKDDIVSAALMXDFSREELSLDLYMTHQELMDQAMINRKYXNQYYIYNAEQFDGPVSDEVLVDAGYNIFEVAKGSINPQSCRYVIHHLVLKFKTLLTLDNKEFSKVMHFWDNFVQTHSESKRALLRNKIAQLFPWRGCSLQGDIMDKKLDELFGDNVVDVLQALIMIWTEFPQGIIPWCSFVEFAKLECNAQYPVDFFYKKFPECLPDHDYTCVXFEFLEMLRHFVTTNAFKCKGKVVDWVTCCAAVCFKMQERDTERRPFKNVEEWFGVRTKHGEALYHIFLSYLRSLYAENKLKDFYLAQEFDIFDNYPSGGMNKSGLGRSSGEQMETSLCISVSPCIDPIEERSASACVTEQIILHCSQAAQQRRYDAIHTFSNKENDFLNAFSMNPAKVFENLVXENTIQCLEIFDPYFNKKLPFCKDLLAQQKNDRSTMREFYFPQDSQYAVASWIDVGFEQDIAEFFLHSLEYPKANGKKTESGAAFVSKIDMNRWLNNTLEYDIFHENIASTVLLQFKTRIGECAQLILFLDGEIKQVQPTGDNIETCEQASSLYSKRTKHNHIEEESASVMCQPSLELIDSLDESRISSMYSHDITNSKXKPVPVRVHPYRGSSVSLLLXEAVENTIAQXQXHFXDEELHYMAFVAGX</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFA7</accession>
    <name>A0A1E5RFA7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">mRNA-capping enzyme subunit beta</fullName>
            <ecNumber evidence="8">3.6.1.74</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">mRNA 5'-phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">mRNA 5'-triphosphate monophosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1861</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">First step of mRNA capping. Converts the 5'-triphosphate end of a nascent mRNA chain into a diphosphate end.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 5'-end triphospho-ribonucleoside in mRNA + H2O = a 5'-end diphospho-ribonucleoside in mRNA + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67004"/>
            <dbReference type="Rhea" id="RHEA-COMP:17164"/>
            <dbReference type="Rhea" id="RHEA-COMP:17165"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:167616"/>
            <dbReference type="ChEBI" id="CHEBI:167618"/>
            <dbReference type="EC" id="3.6.1.74"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:67005"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer. The mRNA-capping enzyme is composed of two separate chains alpha and beta, respectively a mRNA guanylyltransferase and an mRNA 5'-triphosphate monophosphatase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the fungal TPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.74" evidence="8"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFA7">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFA7"/>
    <dbReference type="InParanoid" id="A0A1E5RFA7"/>
    <dbReference type="OrthoDB" id="272147at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031533">
        <property type="term" value="C:mRNA capping enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140818">
        <property type="term" value="F:mRNA 5'-triphosphate monophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004651">
        <property type="term" value="F:polynucleotide 5'-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07470">
        <property type="entry name" value="CYTH-like_mRNA_RTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.100.10">
        <property type="entry name" value="mRNA triphosphatase Cet1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040343">
        <property type="entry name" value="Cet1/Ctl1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033469">
        <property type="entry name" value="CYTH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004206">
        <property type="entry name" value="mRNA_triPase_Cet1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037009">
        <property type="entry name" value="mRNA_triPase_Cet1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28118:SF1">
        <property type="entry name" value="POLYNUCLEOTIDE 5'-TRIPHOSPHATASE CTL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28118">
        <property type="entry name" value="POLYNUCLEOTIDE 5'-TRIPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02940">
        <property type="entry name" value="mRNA_triPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55154">
        <property type="entry name" value="CYTH-like phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="4 8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="mRNA triphosphatase Cet1-like" evidence="10">
        <location>
            <begin position="275"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="41"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="84"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="97"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="11">
        <location>
            <position position="37"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="11">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="562" mass="63832" checksum="F09D2E5BE0CE8601" modified="2017-01-18" version="1">MSSSNRLSLNNLINHEDEDEQKNNMVSESVKNGLVADDTETNSNIKNTDTPAQQQSNGGGVTFGKFDFEYGNDDSEEDADGEQTGEKIEENMAKHDEDDDMDSDDEEFEKDLENELKDGNNDAQDQTPLLEEQDVKQEPXETNPXLNXDTTSGIGSKRPHSPNXKTIKSEANEDKKLKKTXTTNSTIIEDFQNKEVSKTTVKEDIAKLSNLTNISKPVKYNITPMWARKWQVKAKSNANTANNSTSETGNDVSLEDSVNGFPVFLPSYANVIPDDDLTKSVQEWIYATLVSTPEEERPFLELEMKFGKLTGENQVDRINPPISTQSIYTDIDGHLLPNVDSTLFDALTKYFNNQQQQATATGDKKTSFNVIESHTIDSVYRLNDFYNHRRPRFLRLSKDYDTGRIAQFVEKKSIAQLLIYSPKDSYDMKISINLELPLNTNDEPPEKFERETPIHLRNKDRISFIHNNSFTRFDLTHVINKNMNSGNKNLLVANDEQAKNVTNTYEVELEINTPMLLKAFDYISTDSTKYASLIRSYLNNGTLVRRKMSQLSAEIYEGKKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFD5</accession>
    <name>A0A1E5RFD5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">proteasome endopeptidase complex</fullName>
            <ecNumber evidence="4">3.4.25.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g2268</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.25.1" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFD5"/>
    <dbReference type="FunCoup" id="A0A1E5RFD5">
        <property type="interactions" value="618"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFD5"/>
    <dbReference type="InParanoid" id="A0A1E5RFD5"/>
    <dbReference type="OrthoDB" id="37597at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03761">
        <property type="entry name" value="proteasome_beta_type_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000013">
        <property type="entry name" value="Proteasome subunit beta type-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF3">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="9 14" id="KW-0647">Proteasome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0888">Threonine protease</keyword>
    <keyword evidence="10" id="KW-0865">Zymogen</keyword>
    <feature type="active site" description="Nucleophile" evidence="13">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ85611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="294" mass="32167" checksum="93D8B09C237A7CF1" modified="2017-01-18" version="1">MLNAIADQLQGHSSLAFKELSSANTAEEEQEFLENNFINGASRFNSLAPVMELPPISTPAEFLRAHTDDSKNPDCAIKIAHGTTTLAFRFQGGIIVAVDSRATAGQWIASQTVKKVIEINPFLLGTLAGGAADCQYWETWLGSQCRLHELKSRERISVAAASKILGNLVYEYKGAGLSMGTMICGYTKKEGPTIYYVDSDGTRLKGDLFCVGSGQTFAYGVLDSNYKWDLSVEEALYLGKRSILAAAHRDAYSGGSINLYHVTENGWIYHGNHNVDTTFWETKKKEGSFNNVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFD6</accession>
    <name>A0A1E5RFD6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional protein SWT1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1839</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFD6">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RFD6"/>
    <dbReference type="OrthoDB" id="2017974at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18727">
        <property type="entry name" value="PIN_Swt1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052626">
        <property type="entry name" value="SWT1_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16161">
        <property type="entry name" value="TRANSCRIPTIONAL PROTEIN SWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16161:SF0">
        <property type="entry name" value="TRANSCRIPTIONAL PROTEIN SWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13638">
        <property type="entry name" value="PIN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="169"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="472" mass="53925" checksum="00A0FA2B5AF27E0F" modified="2017-01-18" version="1">MDADNDIDFKNFYSVEQKLRGQYNHACLQEXNKHHHQNDGFVETSEEQLHHRKENAPTVSAQIDKALKNFKKFSSNSKYSLQEMENYLQSHVNDNEGDDPMAALNLQNSEYDLVSKYIESERLGNFHDPELADGLKNDALSKAPLAANNEQNFYFNNVSAQTSSNPYQAFFVIDTNVLISNLKTLEELRAHANTGLTYQIIIPSVVLKEVDSLKKXSREETLFQHESQHAGRQRGNPVSIQKSSKAANDWLYHNLARKDPSLKVQKSTETLNTPNQINNDDAILDCCLYFKKKVEGTPITVVLISNDRNLCIKCLSEEVQTISFIGKNIITTENIIDITKRQFAMNSVEILANSVKSDALRLLNFVTEAFFPHNESFLSLLDFIKYVXIHWDANNGGVQSLFLSDPVFMHLQYWVTNXHSITTAVHDNMEAFTLCQCWSKVLTLLYSXFXQDQTVLQNCFARWEKLINTLPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFE7</accession>
    <name>A0A1E5RFE7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved oligomeric Golgi complex subunit 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2266</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFE7">
        <property type="interactions" value="831"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFE7"/>
    <dbReference type="InParanoid" id="A0A1E5RFE7"/>
    <dbReference type="OrthoDB" id="47059at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048682">
        <property type="entry name" value="COG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048684">
        <property type="entry name" value="COG4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013167">
        <property type="entry name" value="COG4_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24016">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24016:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20662">
        <property type="entry name" value="COG4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08318">
        <property type="entry name" value="COG4_m"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00762">
        <property type="entry name" value="Cog4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COG4 transport protein middle alpha-helical bundle" evidence="1">
        <location>
            <begin position="217"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="867" mass="100240" checksum="CA4569E589119691" modified="2017-01-18" version="1">MTTSQNFDEDTLHAKLENFETILYKTLKTQDQAAKLLQALKDEHEHTYTDLINYINNTVITNEQDFGRTLELNKTSLSSVLSRFHSSLKSNKYEKNYQISTKIQEILTSLTTEYESVQSTYNYLNNLQKIKQKCKQIHQDFDPNSSRQKYDNVNNNKKLLEASQNIRDILSLINNDFEYLNTDFARKIIPTSDLPMEPVSFITKTMNDILVINKNVFDQSCEKLYHDPNDNKNLQILQQSFQIFPLINQPKLGLDLYSKYICNIINDKSRDILVLQKKKLSIPQSFLHLFKMCSMIINNHSQIIIRFYNNDKKSMISIMDNLQKEIDLQSSLILDIFTDKSDLQKRKDEFLSDFLIILGYWSMFQKFFSIKYKEFSDDAEPVTRDSTTEENSEPSVTHIPKCLAENQFNSKLNAIINESFKPLFQQQIKXNLGKVFDIEIMPDLNNYISSFSKPDPQLIDAPVPTSSLTDDFSIICKKYLIIIINSGDFELVDEFMSDFLPDVIIKELFIKKILNKLADLSITPTYLCKYTDSLEKFGARGSTPTPQHENQSGLINTRDYVMKSAFSSIQQYANTIKGSATSNIVKEVFSNKQIVADEYSTDIGMHHFHTLLIYMNTVQALKVVLEKLLVEELINGNNILPTNFIFEKHADSVANKIKNAYHASVDYCDLILKNYASKIFEIGWKQACQSLGKLLLNGKHNFIHQSAEDFNQSQKEIPQAYFCNAADLEDLGTLADFIKFWNIISQPYKNVCDPKFYSVILSYVIDYINTSVLLPKFWGLKKVNELGALKLDKEISLCISTVCSSDHYYLREKFNKLNQLILVLGFEMEDFATNKETGMIELKTELKDSITWCITDEEIHRVRKMRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFT2</accession>
    <name>A0A1E5RFT2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1,3-beta-glucan synthase</fullName>
            <ecNumber evidence="3">2.4.1.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">1,3-beta-D-glucan-UDP glucosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g2384</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[(1-&gt;3)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;3)-beta-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21476"/>
            <dbReference type="Rhea" id="RHEA-COMP:11146"/>
            <dbReference type="Rhea" id="RHEA-COMP:14303"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37671"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.34"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.34" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFT2">
        <property type="interactions" value="673"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFT2"/>
    <dbReference type="InParanoid" id="A0A1E5RFT2"/>
    <dbReference type="OrthoDB" id="1880850at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000148">
        <property type="term" value="C:1,3-beta-D-glucan synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003843">
        <property type="term" value="F:1,3-beta-D-glucan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006075">
        <property type="term" value="P:(1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051278">
        <property type="term" value="P:fungal-type cell wall polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026899">
        <property type="entry name" value="FKS1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056261">
        <property type="entry name" value="FKS1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003440">
        <property type="entry name" value="Glyco_trans_48_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741:SF48">
        <property type="entry name" value="1,3-BETA-GLUCAN SYNTHASE COMPONENT FKS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741">
        <property type="entry name" value="LYST-INTERACTING PROTEIN LIP5 DOPAMINE RESPONSIVE PROTEIN DRG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14288">
        <property type="entry name" value="FKS1_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23605">
        <property type="entry name" value="FKS1_dom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02364">
        <property type="entry name" value="Glucan_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01205">
        <property type="entry name" value="FKS1_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="557"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="599"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="628"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="742"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1432"/>
            <end position="1453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1519"/>
            <end position="1539"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1545"/>
            <end position="1563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1635"/>
            <end position="1658"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1670"/>
            <end position="1695"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1716"/>
            <end position="1736"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1742"/>
            <end position="1767"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1873"/>
            <end position="1893"/>
        </location>
    </feature>
    <feature type="domain" description="1,3-beta-glucan synthase component FKS1-like" evidence="13">
        <location>
            <begin position="366"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="13"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="87"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ85443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1950" mass="223407" checksum="70411E3E83FE5476" modified="2017-01-18" version="1">MSYPQNGEDHQYYQDNQNQQHPDGFDQPQDQQYVDPSQQQYVDPSQQQYADPSQQQYADPTQQQFYDAQVDQYGNPIAYDQYGNPSYDPNQTQQPPQNGAGQGQYYYDQPRGNFPDPNTSENFSDFSYGPNGYSQNNAGANNGAMGPGTGYDQGFAPSQSQMSINNPAGGNGFYSQPQPGYTMSSSGTSTPYTGSASGMMYDPIAMALPNDPYPAWTADPQSPVSIEQIEDVFIDLANRFGFQRDSMRNQFDNFMTLLDSRASRMSPDQALLSLHADYIGGDTANFKKWYFAAQLDLDDQVGFRNMSLGKLSRKARKLKKKQKKQMEEQIDQQENPEEIINQLDGDNSLEAADFRWKAKMNLLTPLERVRHVALYLLCWGEANQVRFTPECLCFIFKCCLDYLESPLCQQSTTPMPEGDYLNRVVTPIYKFLRSQVYEVVDGRYMKREKDHNKVIGYDDVNQLFWYPEGLTRIILNDGTKITDLPSEERYLKLGEIPWDFVFFKTYKETRTWFHLATNFNRIWIIHACVYWMYLAYNAPTFYTHNYQQLKNNQPTPAYRWASAALAGTLACVIQILATICEYFFVPRQWAGAQHLSRRLYFLLGALAVNLGPIIFVFAYQPINVYSKLAYAIAVVFFFVAVATMVFFSVMPLGGLFTSYMNKSSRKYVASQTFTASFAPLKGLDMWMSYLVWFTVFGAKYSESYFFLVLSLKDPIRNLSTMTMRCTGDYWFGNTLCRIQPKIVLGLMIATDFILFFLDTFMWYVIVNTVFSIGKSFYMGISILTPWRNIFSRLPKRIYSKILATNDMEIKYKPKVLISQIWNAIVISMYREHLLAIDHVQKLLYHQVPSEIEGKRTLRAPTFFVSQDDNNFETEFFPKDSEAERRITFFAQSLATPIPEPLPVDNMPTFTVITPHYSEKILLSLKEIIREDDQFSRVTLLEYLKQLHPVEWDCFVKDTKILSEETAAYENTMNQEDGEVNDEEKENSLKTQIDDLPFYCIGFKSAAPEYTLRTRIWASLRSQTLYRTISGFMNYARAIKLLYRVENPEIVQMFGGNAEGLERELEKMARRKFKFVVSMQRLAKFKPHEMENAEFLLRAYPDLQVVYLDEEPPLNEGDEPRIYSSLIDGHCEILPNGRRRPKFRVQLSGNPILGDGKSDNQNHAIIFTRGEYLQLIDANQDNYLEECLKIRSVLAEFEELNVEQVNPYAPELKYEEQSSNHPVAIVGAREYIFSENSGVLGDIAAGKEQTFGTLFARTLSQIGGKLHYGHPDFINTIYMTTRGGVSKAQKGLHLNEDIYAGMTAILRGGRIKHCEYYQCGKGRDLGFGTILNFSTKIGAGMGEQMLSREYYYLGTQLPLDRFLSFYYAHPGFHLNNLFIQLSLQMFTLTLVNLNALAHESIICFYNKNKPVTDVLYPLGCYNLAPAIDWIRRYTLSIFIVFFIAFIPIVVQELIERGVWRATQRFFKHILSLSPMFEVFSGQIFSNALLSDMTYGGARYISTGRGFATSRIPFSILYSRFAGSAIYMGSRSLLMILFGSVAHWQPALLWFWASLSSLMFSPFIFNPHQFSKEDYFIDYRDFIRWLSRGNSKYHRNSWIGYVRMSRSRVTGFKRKVLGEESEKTIGDAPRAQRSNIFIAELLPCAIYAAGCFIAFTFINAETGVRNVEPVNSVLRLIICTLGPIAINMGILFFTLGLSCCSGPLFGMCCKKTGPVMAAFAHGFAVVVALIGFIVMWVLCGFNFARALIGQIAVIFCQRFIFICMSSLLLTREFKNDHANTSFWTGKWYGSGFGYMAWTQPWREFVAKIIELSEFAADFILGHILLFCQLPLIMMPFMDKFHSVMLFWLKPSRQIRAPIYSLKQTRLRRRMVKKYSVLYFTVLGIFVACIVGPAVASSSKQLKTLGKSFTGVFVGLVQPRHQDKNDSKVVSTMKNAKNFYSTPKIKSFSTKTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGF9</accession>
    <name>A0A1E5RGF9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Protein ECM9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1377</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ85999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGF9">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RGF9"/>
    <dbReference type="OrthoDB" id="5358702at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ85999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="392" mass="45781" checksum="3F13CC98149C1798" modified="2017-01-18" version="1">MKFNNDSAMTXVPETFALNESIRNTLCYQFFEHYSRYFVEYTNIQLEFYPKNPETSSPXPFIAVADENDNLXIVWFQTARLQLFSESFKFFQDSSTDLQQFSAFDLFIMTLGKLMTTPNDQTNLKRHEKYMLEILEQSPSXLNVNQSLVGLLLSSTDQRLNKSSMLWFYYKKLVVLKKQNSTSPDLGDHVEQVCLKSAKNHFANYYCWNTIRWIFDNELNDNKKSAIFSQVFQFCRSNFTDCSAWDTLAYFSKQSSLKQYSSYNVISHRRIQRLLKLHVGAQGASDQRLYWKDQNIVIATAQEVMHMIRTLERASIPVFNYLLSVVTLIPPTIFTAELSLLKASLRADVSQDQKDKKGHENLVTLQQAEYVEQLMEFLEKLEKDKTLLHLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGG6</accession>
    <name>A0A1E5RGG6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Kelch repeat-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1087</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGG6">
        <property type="interactions" value="524"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGG6"/>
    <dbReference type="InParanoid" id="A0A1E5RGG6"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061245">
        <property type="term" value="P:establishment or maintenance of bipolar cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244">
        <property type="entry name" value="KELCH REPEAT DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244:SF456">
        <property type="entry name" value="MULTIPLE EPIDERMAL GROWTH FACTOR-LIKE DOMAINS PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="603"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="795"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="853"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="950"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="33"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="81"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="123"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="182"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="613"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="857"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="3">
        <location>
            <position position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1054" mass="116299" checksum="13E556B0765486D1" modified="2017-01-18" version="1">MAPFKFGKIKSHLTSSGPPAAAPPPPPSANPSTQTPNSFHSTTSNHGEPTPLYQNQPSFASHSQFNTPXXSYEQKNDSAKFSDQSFSSLSNSPKPTSGSYKQQTSIAPSSRSPAGEXVRSPISLMNKQNKYGSAYSSHENGFAHSPRQERNVSGSAFVGNNSPQLNSNNPFASGMQRKASNEPATPRSQQRVPSVNRSSSPWXRIKLANSPFPRYRHVASLHATHDKNEIYVIGGLHEQSVYGDTWVLKYSKPSDFSSKETAGLVFHSKTVDISDQTPPPRVGHASTLCGNAFVVFGGDTHKMNKNGLMDDDVYLLNINSMKWTIPQQSGPRPLGRYGHKINVIQSLSSEKNATTTKLYVFGGQFDDTYFNDLCCYDLSSFRKPDSHWVFLKPATFIPPPITNHSMCVFDNKLWVFGGDTAQGLLNQVFVYDPFKNDWTVVPTKPANXQVDAELPPPMQEHACSVYKNLMCVFGGKDENDIYSNDLWILNMKTLEWWKFPNLQGKDQYMPSARSGHSLTLLPKENLLLVMGGDKMDYCASPDPRNFRVDEEQDCGXGSILYTFDLGSLEKHCPGIFDVVANSTSELNSLNSTLLLDKHLEAGESEQDEAVDQTPRNASHNSMSTTEGXENFHTPXAYGVHRTIDMNKGLRXQDDPGDDFNEXXEVANSTITEEQHENGDITNDSVQSDISKEKIGDEELGTDPRRATEIAEKANQRGVSDFFDTYTDESASVMLRGSYANSATTPLKGAADSRTSSMLLNNMLSSNAHILTQDAFVRTSGGETNMDVSPVGELKRHDKFGLEPPNVVDRPASQARTDSLSLDEDIQLQTANTMVKRNAGFGKGPVLVGLGNEANGKTVNENANDTQNNENADTTDDKENMEAEDSVLNDHEFLNSVEESKELGAAPIVGLEEPAIIDKXVDXANSANDKQPXFESVDILDQLAKEMMQIKQDTMEQARKASERIKELETENAALKESNLQQDTRAVGSADVSXSAVNHEFEQKYEVLSEKHELLQAELDFAHTEISKIKKLYNEERKKLALILKEAATSLETDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGN1</accession>
    <name>A0A1E5RGN1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Lipase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1563</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the putative lipase ROG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGN1"/>
    <dbReference type="FunCoup" id="A0A1E5RGN1">
        <property type="interactions" value="58"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGN1"/>
    <dbReference type="InParanoid" id="A0A1E5RGN1"/>
    <dbReference type="OrthoDB" id="5592486at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11440">
        <property type="entry name" value="LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF676" evidence="2">
        <location>
            <begin position="179"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="377" mass="42743" checksum="D27BF0A9A1624EC3" modified="2017-01-18" version="1">MFGLLKKKSTLLGLGAAFGSGTVFYNTSTNSTLSNNTGNFVPSKNKSDNIDYIIKKIDPIEEFHAPKNPVVLCHGLSGFSRLILLPSVYQITKFFRNFISITKDDQQLMHNIYDDDDDPNYKAFLEIDYWIGVKAMLEAQNVTVFTAKVPPFGSIDERAYALNDFIRAKIFYMKRSETVPEIKDNESKVKVNLIAHSMGGLDARYLISAIPPEEKQNYEVLSLTTISTPHHGSVMADYVCNLFDDLKSRMGVENPNVKLPVAFYQLTTDYVDLIFNKQIKDDPKVKYFSYGAYYNPNWSRMFYFSWKTIMTLSNEPNDGLVTIASSKHGKFRGVLPNLDHLSIINWNVSLTGKKDPNAIDILQFYMKVVDDLGRCGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGS5</accession>
    <name>A0A1E5RGS5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription initiation factor IIF subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1144</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TFIIF alpha subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGS5">
        <property type="interactions" value="176"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGS5"/>
    <dbReference type="InParanoid" id="A0A1E5RGS5"/>
    <dbReference type="OrthoDB" id="76676at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005674">
        <property type="term" value="C:transcription factor TFIIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001096">
        <property type="term" value="F:TFIIF-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008851">
        <property type="entry name" value="TFIIF-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011039">
        <property type="entry name" value="TFIIF_interaction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13011:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13011">
        <property type="entry name" value="TFIIF-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05793">
        <property type="entry name" value="TFIIF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50916">
        <property type="entry name" value="Rap30/74 interaction domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="174"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="420"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="620"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="174"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="217"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="231"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="443"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="470"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="494"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="507"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="541"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="567"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="584"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="635"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="719" mass="81011" checksum="C1DAA47ECFC1BD32" modified="2017-01-18" version="1">MASSQSPPVXRPGSTSPAAVKKKRKAASPFIKRDHFRRQFLKSKMKNGVNGGVKKEEDPDAKANSLLKVKMDPYVKDEYDAIKKTERKDFPLQDANGNQEDEYDEYPLRAVDADAIMGIKNNIMKFQSKKPMIPEKNLHLPVRLHRKDTRNLQFQLTRAEIVQRQKEIAEYKKTMVEKESGKGGNKNEKTGNGKNEKGEDIFTTASGASITMSKPEASNAKNQTNAAATTGSSDAAQSNGDQPNAEEPVRKQEAGKVLYDGQETFEGEFEEGTIDPFADVAPDGGARAKRGWAGKRKTRKLRLLDENAKKLRFEEYYPWVLEDFDGYNTWVGSYEAGNSDQYVMFAVQKDGSFTMLPADKVYRFTARSQYATLTIEEAEKRFEKNKNVVPRWLMKHLDDIGTTTTRFDRTMRKLKAVDGMKKDGEDDDDDMDGLFGSRKDNSDNELDYDEEFQDDEEAPIIDGNEEENKESEQRLKKEMLQANAMGLRDAGSFPEDEEEEEDVEIDAEGKEVKKALRKTDLGALYDSDEEKNPYLSESDVEENKKEEEENSKSKEASMKADGDGSGKQATNGKSANEGTPKNKSPSKQRSGPKKVKKIKTSCKKYKNGVVLIKADPTILKNFPAGEWNPGKNEDSSVQQSSLKTEGAQAQQQQSLGTKEDRPLTEQDIIDIVSSGKITLKELIKELKRRITMDTENRERMRVYVKKLVKISDGFLELSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGT4</accession>
    <name>A0A1E5RGT4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">4a-hydroxytetrahydrobiopterin dehydratase</fullName>
            <ecNumber evidence="3">4.2.1.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">4-alpha-hydroxy-tetrahydropterin dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1312</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin = (6R)-L-erythro-6,7-dihydrobiopterin + H2O</text>
            <dbReference type="Rhea" id="RHEA:11920"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:43120"/>
            <dbReference type="EC" id="4.2.1.96"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pterin-4-alpha-carbinolamine dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.96" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGT4"/>
    <dbReference type="FunCoup" id="A0A1E5RGT4">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGT4"/>
    <dbReference type="InParanoid" id="A0A1E5RGT4"/>
    <dbReference type="OrthoDB" id="277398at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008124">
        <property type="term" value="F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00488">
        <property type="entry name" value="PCD_DCoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.20">
        <property type="entry name" value="Transcriptional coactivator/pterin dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036428">
        <property type="entry name" value="PCD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001533">
        <property type="entry name" value="Pterin_deHydtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599:SF0">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01329">
        <property type="entry name" value="Pterin_4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55248">
        <property type="entry name" value="PCD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ86118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="111" mass="12637" checksum="5FD76827E6E94829" modified="2017-01-18" version="1">MYNKIVKTVPVLINAHNAGLLTKIPTWLVISNDKTEYHKLTKTFTLKNFEATWGFLTQIAMRSHLSGHHPTITTTYTNVKIELNTHDMGNAITDIDIKLATKIDSYEKSYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH55</accession>
    <name>A0A1E5RH55_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 14">6-phosphogluconate dehydrogenase, decarboxylating</fullName>
            <ecNumber evidence="10 14">1.1.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g1050</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10 14">
            <text>6-phospho-D-gluconate + NADP(+) = D-ribulose 5-phosphate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:10116"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="EC" id="1.1.1.44"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10 14">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10 14">Belongs to the 6-phosphogluconate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.44" evidence="10 14"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RH55"/>
    <dbReference type="FunCoup" id="A0A1E5RH55">
        <property type="interactions" value="962"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH55"/>
    <dbReference type="InParanoid" id="A0A1E5RH55"/>
    <dbReference type="OrthoDB" id="434986at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00410"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004616">
        <property type="term" value="F:phosphogluconate dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019521">
        <property type="term" value="P:D-gluconate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000002">
        <property type="entry name" value="6-phosphogluconate dehydrogenase, decarboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.320:FF:000002">
        <property type="entry name" value="6-phosphogluconate dehydrogenase, decarboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000007">
        <property type="entry name" value="6-phosphogluconate dehydrogenase, decarboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.320">
        <property type="entry name" value="6-Phosphogluconate Dehydrogenase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006114">
        <property type="entry name" value="6PGDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006113">
        <property type="entry name" value="6PGDH_Gnd/GntZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006184">
        <property type="entry name" value="6PGdom_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006183">
        <property type="entry name" value="Pgluconate_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00873">
        <property type="entry name" value="gnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006765">
        <property type="entry name" value="PRK09287.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11811">
        <property type="entry name" value="6-PHOSPHOGLUCONATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00393">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000109">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00076">
        <property type="entry name" value="6PGDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01350">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00461">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0311">Gluconate utilization</keyword>
    <keyword evidence="5 10 14" id="KW-0521">NADP</keyword>
    <keyword evidence="6 10 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 10 14" id="KW-0570">Pentose shunt</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase C-terminal" evidence="15">
        <location>
            <begin position="181"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="35"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="77"/>
            <end position="79"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <begin position="131"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <begin position="188"/>
            <end position="189"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="452"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="458"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000109-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000109-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ86204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="495" mass="54378" checksum="84D92BACC3851331" modified="2017-01-18" version="1">MSSPKGDIGLIGLAVMGQNLILNAADHGFTVVAYNRTVSKVDDFMANEAKGKSIIGAHSIKELCENLKRPRRIILLVKAGKPVDLFIEQLLPYLEKGDIIIDGGNSHFPDTNRRYEELEKQGILFVGSGVSGGEEGARYGPSLMPGGAEEAWPHIKDIFQSIAAKSDGEPCCDWVGPAGSGHYVKMVHNGIEYGDMQLICEAYDIMKRLGKFTNQEIGDVFEKWNKGVLDSFLIEITRDILKKNDDDGKPLVEKILDTAGQKGTGKWTAINALDLGMPVTLIGEAVFARALSALKDERVEASKILGGPTIPEDTSKIFTDKAKFVDDLEQALYASKIISYAQGFMLIREAGKTYGWKLNNPAIALMWRGGCIIRSVFLAEITKAYRENPELENLLFNKFFTEAITKAQSGWRKTIGLATEFGIPTPAFSTALSFYDGYRSKRLPANLLQAQRDYFGAHTFKVLPECANDHLPAGQDVHFNWTGHGGNVSASTYQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHN1</accession>
    <name>A0A1E5RHN1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">RNA exonuclease 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1022</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the REXO1/REXO3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHN1"/>
    <dbReference type="FunCoup" id="A0A1E5RHN1">
        <property type="interactions" value="239"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHN1"/>
    <dbReference type="InParanoid" id="A0A1E5RHN1"/>
    <dbReference type="OrthoDB" id="206335at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06145">
        <property type="entry name" value="REX1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000019">
        <property type="entry name" value="RNA exonuclease NEF-sp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034922">
        <property type="entry name" value="REX1-like_exo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF115">
        <property type="entry name" value="FI18136P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exonuclease" evidence="9">
        <location>
            <begin position="478"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="108"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="232"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="39"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="108"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="820" mass="91280" checksum="185B0F42B578331F" modified="2017-01-18" version="1">MSRRVARKLKKKRSQKKHGGLQKGEDSNTNGLTPNHSSTNTKGKTNNTKVVTPVKKRILNLQATKKLPGQTSPFLDSSPKVQNNQSEKGFPPSYEERMHELLHLAQEDASSGQQDNDGFSDSHEQMEKESSPSSSVASSVGSIITPISSSALESTVLYNNGEHRDRYSDEETFLNRHAPLKAKDSSLENNSNNVPSMNNENLDAVGTSSDYSTVASRSNSVSLEAGEVAMKNLNAKRRRRSSAVAPSPGNNLKSPPTLKHETSLSSIAAPKKKKRLLRPATISLTSKTQGKRMSVKDLRDLVLYILESKTNCPSWFHIENKSNITKIITLFVPGLEPQDLAHFSSQEKPLSFSDLTSGSEKNTELAPDFLDPSIKFSVASVQTPGSKNSIYSSYNSFLNVGLTKLKKQQLREEMNRKKITINDLLLNVGDLIAAAYPVHPETPNIPESLVQQIETRNANLPDSEEWVETKELHQEAPRTFALDCEMCLSANGHVLTRCSVVNFQNEVVYDQLVKPDVEIVDYLTKYSGITEEMLRPVTCTAKQVQQDLLQLISLDDVLIGHSLQSDLNILKLRHTKVVDTAFIYEHKAGPPFRPSLKYLSQEYLNVNIQNDDAKGHDSITDARTCMDLTKLKIQNGLAFGMGLNTESLFKKMYESTRIRSVTFTDYAPEFSYDGEANKDNCKSVKCVSDKEIFEKLGKNLSDHDFFVARLRDLEFERGYAQQRSSVPDGSNCTSQFASRESAIEGLKNGLSTLYEQIPTSALVIIFTATGDVTDYKRISQELNAITIKEERQKAQSLRQAELSEAVTKARDAMAMFITKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHN7</accession>
    <name>A0A1E5RHN7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Signal recognition particle subunit SRP21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1454</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHN7"/>
    <dbReference type="FunCoup" id="A0A1E5RHN7">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHN7"/>
    <dbReference type="InParanoid" id="A0A1E5RHN7"/>
    <dbReference type="OrthoDB" id="5419752at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039432">
        <property type="entry name" value="SRP9_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05486">
        <property type="entry name" value="SRP9-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP9" evidence="1">
        <location>
            <begin position="10"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="193" mass="21479" checksum="742A3FA19732F22E" modified="2017-01-18" version="1">MPATPINYPVKPVDTFIGSSVKTLEASPSDTLITMKYDFNKKFKSVKNTSDKKEHYKTSVEFKTSNNALDLHYKFSTNKIKELSRVLSALGPNGVSMNPVTNKVLKKKHFVETTSKVNKKKKSSYLKNLGKSSKKPVKYSIKHDALSKLPGAGTLLANTEIKKYVERKPIVASGGEVTKGKKKLKSKSKSKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHV8</accession>
    <name>A0A1E5RHV8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">Adenylyl-sulfate kinase</fullName>
            <ecNumber evidence="4 12">2.7.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g1383</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the synthesis of activated sulfate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>adenosine 5'-phosphosulfate + ATP = 3'-phosphoadenylyl sulfate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24152"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the APS kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.25" evidence="4 12"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHV8"/>
    <dbReference type="FunCoup" id="A0A1E5RHV8">
        <property type="interactions" value="194"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHV8"/>
    <dbReference type="InParanoid" id="A0A1E5RHV8"/>
    <dbReference type="OrthoDB" id="506431at2759"/>
    <dbReference type="UniPathway" id="UPA00140">
        <property type="reaction ID" value="UER00205"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004020">
        <property type="term" value="F:adenylylsulfate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019344">
        <property type="term" value="P:cysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070814">
        <property type="term" value="P:hydrogen sulfide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02027">
        <property type="entry name" value="APSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000212">
        <property type="entry name" value="Adenylyl-sulfate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00065">
        <property type="entry name" value="Adenylyl_sulf_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002891">
        <property type="entry name" value="APS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059117">
        <property type="entry name" value="APS_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00455">
        <property type="entry name" value="apsK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003013">
        <property type="entry name" value="PRK03846.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11055">
        <property type="entry name" value="BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11055:SF1">
        <property type="entry name" value="PAPS SYNTHETASE, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01583">
        <property type="entry name" value="APS_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="8 12 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="APS kinase" evidence="13">
        <location>
            <begin position="24"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ86464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="198" mass="22599" checksum="739A1B78A94DB7EA" modified="2017-01-18" version="1">MATNITWHPNLTHDERKGLRKQEGVTIWLTGLSASGKSTIACALEQLLLQENVNAYRLDGDNIRFGLNKDLGFSENDRNENIRRISEVSKLFADSCAVSITSFISPYRADRDKARELHRESGLKFIEVYVDVPLEVAEQRDPKGLYKKAREGIIKDFTGISAPYEEPSEPEIHLRTDKLTVEEAAEKIYEYLKQEKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI41</accession>
    <name>A0A1E5RI41_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">FACT complex subunit POB3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1198</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the SSRP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI41"/>
    <dbReference type="FunCoup" id="A0A1E5RI41">
        <property type="interactions" value="1058"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI41"/>
    <dbReference type="InParanoid" id="A0A1E5RI41"/>
    <dbReference type="OrthoDB" id="498543at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035101">
        <property type="term" value="C:FACT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13230">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13231">
        <property type="entry name" value="PH2_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13229">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.150:FF:000001">
        <property type="entry name" value="Fact complex subunit ssrp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000098">
        <property type="entry name" value="Fact complex subunit ssrp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.220">
        <property type="entry name" value="Structure-specific recognition protein (SSRP1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050454">
        <property type="entry name" value="RTT106/SSRP1_HistChap/FACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048993">
        <property type="entry name" value="SSRP1-like_PH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000969">
        <property type="entry name" value="SSRP1/POB3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035417">
        <property type="entry name" value="SSRP1/POB3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024954">
        <property type="entry name" value="SSRP1_DD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038167">
        <property type="entry name" value="SSRP1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849:SF1">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21103">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17292">
        <property type="entry name" value="POB3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03531">
        <property type="entry name" value="SSrecog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00887">
        <property type="entry name" value="SSRCOGNITION"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone chaperone RTT106/FACT complex subunit SPT16-like middle" evidence="12">
        <location>
            <begin position="393"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="188"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="495"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="209"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="528"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="550"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ86561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="577" mass="64909" checksum="20A3417B91CCB129" modified="2017-01-18" version="1">MSNSTDFDKIYLNQGKISGRFRIADSGLGWRSSQKGGSTATANPEKLKPFLLSADELSSIQWSRGCKGYELKINTRNEGVVQLDGFNQEDFNLVKNEFLRRFNITVEVKEHSLRGWNWGQTDMARNEMVFSVNGKPTFEIPYSRINNTNLTSKNEVAVEFQINNETYQPAGDEIVEMRFFVPSTIIKGEGEDEDEDMDKEGQKVKGEGEEGNDGSDQENEDEDAAIVDRKTMAESFYEELKAKADIGEVSGDAIVSFQDVFFTTPRGRYDIDIYKNSIRLRGKTYEYKLQHNQIQKIFSLPKADDLHHNMVLAIDPPIRQGQTTYPFLVLQFDNNEETEVQLNVEDDEFEELYKDKLMKQYDAKTHMVLSHVLKGVTGRKVIVAGGYVSKFEQAAVSCSLKANEGYLYPLENSFLFLTKPTLYIPYSDISLVSISRAGDSSSTARTFDLEIILRGNKGTTTFGNISKEEQQGLETFLKSKNLRVKNEEKEVQHRLNTVLAGSDDDSDINMGSAGEEDETPDEDFHVSDDEDGDVAEEFDSDAPVSGSESEAGDPDADDDVIGDDDLDEDRPAKKQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI94</accession>
    <name>A0A1E5RI94_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative mitochondrial membrane protein FMP10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RI94">
        <property type="interactions" value="81"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI94"/>
    <dbReference type="InParanoid" id="A0A1E5RI94"/>
    <dbReference type="OrthoDB" id="506431at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052061">
        <property type="entry name" value="PTE-AB_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47260">
        <property type="entry name" value="UPF0644 PROTEIN PB2B4.06"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47260:SF1">
        <property type="entry name" value="UPF0644 PROTEIN PB2B4.06"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="285" mass="32278" checksum="E6D815515504CB89" modified="2017-01-18" version="1">MMSARSLLSKRCIVSLNKQIPVSTPRFSPLQISCRLQSTSSVQYKKNVQFTTTGNQSNKNNDNVNGIKKFLIFAGFFGIGWFVTQHFTFMDLIAMYRYDKLPETDAKVVEYKTQLKLKLLNLPILKQLEDHGYMKTYDSSSSHNLIDETLNVPGGIFIXPEFYYNPKENKSCGVYHLGMKLTGYPFIVHGGVLATVMEDMMTKALKFEVANGSLSSEGPKSKEHISKLNKISLKYKFPTFANQFVVLRSTSLKKINKNTYEIEIELLDQSGKHALVNGKGKFTIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RMY3</accession>
    <name>A0A1E5RMY3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g631</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RMY3"/>
    <dbReference type="InParanoid" id="A0A1E5RMY3"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="368" mass="42433" checksum="A2DE256FFEE73629" modified="2017-01-18" version="1">MSRDSRVTNPRITSLSSAYKNHGNTNNDTKPSVSNRSKIRVRSIDEIGMPYFPPEVRQQRQLEQQRQHQNQDLYSRYALEENESIETNGKREQSAPSGSIDHAKPTSRPYGHPYQDLEQPHLRLKSEESIVEHFAVHGGGDVQSRSSNVQNAVELRKKVLATKRVIGRPIPLSYVLQKQRNQNRIYNTQNKESHFDSSFGADNEEPSRKPSSTSEKKQEPPKLAFLKQTQDDENTTVNNNHISSSLAQKDSATMESENVPDKFMAEGKQEYSILINNDKDLIDLWECIVANYNKRKANNKRTDTDIQKLHAPSISNEIKRFKKFKAMFTRFKTILGVLFNRNNLFLVMIFVTLLIYNVTIYYMYSGKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN42</accession>
    <name>A0A1E5RN42_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA wybutosine-synthesizing protein 2</fullName>
            <shortName evidence="2">tRNA-yW-synthesizing protein 2</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g870</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">S-adenosyl-L-methionine-dependent transferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the transfer of the alpha-amino-alpha-carboxypropyl (acp) group from S-adenosyl-L-methionine to the C-7 position of 4-demethylwyosine (imG-14) to produce wybutosine-86.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-demethylwyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36355"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10378"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73550"/>
            <dbReference type="EC" id="2.5.1.114"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">tRNA modification; wybutosine-tRNA(Phe) biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN42"/>
    <dbReference type="FunCoup" id="A0A1E5RN42">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN42"/>
    <dbReference type="InParanoid" id="A0A1E5RN42"/>
    <dbReference type="OrthoDB" id="2387925at2759"/>
    <dbReference type="UniPathway" id="UPA00375"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102522">
        <property type="term" value="F:tRNA 4-demethylwyosine alpha-amino-alpha-carboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026274">
        <property type="entry name" value="tRNA_wybutosine_synth_prot_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF25">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038972">
        <property type="entry name" value="Trm12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="4">
        <location>
            <begin position="176"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00049400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR038972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ88308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="477" mass="54932" checksum="2E1601C1C08F0EAF" modified="2017-01-18" version="1">MFIVEVQDKRYVKELKTLLELYNGNSLVKPIKTREKHSDVDGKSVKLFQLKTSITNVEELQKLLENYENCVSIAECADSENISSTSGVNFTITLLDYIRAYLAAQESYSSEFIEDVIANHCSPKYSLYPPLILFNHSPVKQMFISALWQEQIFKNSDETRTHFFEQMIQQVFIPTSSQNGVEFSHVAVNNPIQKADNIWRKPLSIIPLFGDFSTKDVNFDNPTHENFEKGFWCSTVQNGIYQEWAPIYTMFSRGNIKEKARILVTEKTKSTLCHFKDIANNDVLDMYCGIGYFTLSYLKRDCRYLFGFDLNPWSCEGLRRALTSKKNSKFVKQNSCYIYNESNEFAMQRLKDFEASSISTADNNCKLKIRHINMGLLPTSEPGWAVALDVAYRFHGSCLVTTLHVHENIHNDEVKDGSFVNEKLLPALLSFKKQNHYNNIESISVTHVEKIKTFAPDVWHVCIDTDIHFKCVSETCM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN67</accession>
    <name>A0A1E5RN67_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nucleolar protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g641</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN67"/>
    <dbReference type="FunCoup" id="A0A1E5RN67">
        <property type="interactions" value="366"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN67"/>
    <dbReference type="InParanoid" id="A0A1E5RN67"/>
    <dbReference type="OrthoDB" id="167718at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="102"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="215"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="18"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="78"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="215"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="231"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="245"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ88330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="266" mass="30046" checksum="771FFEDD5A062163" modified="2017-01-18" version="1">MSETKLTKKQLKSQNFKKSKDERDQEKALKRKHEDETSTETSKEISKEISKETLKETPTTKTKDITITSTENNSEPVKKKRKTRRGKKGKGNGGAANKGNRFLIFVGNLPYNVTKAELMNHFKNCSPDEIRVRPDKGIAFLEFDPASSDSQKLDTDEKDKNNKSTIKASNIQKRMDIALLQNKTMFQDRKINVELTAGGGGNSENRLSKIKTKNEKMEKERVERIDKMIKNNRSSASTASQDSKTSGNTSTTNTSGIHPDRLKMMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNE7</accession>
    <name>A0A1E5RNE7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Carbonic anhydrase</fullName>
            <ecNumber evidence="2 8">4.2.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Carbonate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g843</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the beta-class carbonic anhydrase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="2 8"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNE7"/>
    <dbReference type="FunCoup" id="A0A1E5RNE7">
        <property type="interactions" value="107"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNE7"/>
    <dbReference type="InParanoid" id="A0A1E5RNE7"/>
    <dbReference type="OrthoDB" id="10248475at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071244">
        <property type="term" value="P:cellular response to carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1050.10">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001765">
        <property type="entry name" value="Carbonic_anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036874">
        <property type="entry name" value="Carbonic_anhydrase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11002">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11002:SF76">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00484">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00947">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53056">
        <property type="entry name" value="beta-carbonic anhydrase, cab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601765-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ88405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="211" mass="24001" checksum="CD832D0906FF13FC" modified="2017-01-18" version="1">MCTMCEPTAPFKLTHSSSLDDYLSNNQVWSSNMQQTYPTLFTEYNSKGQDPHTLFIGCGDSRYNENCLGVLPGEVFTLKTVGNNINTGDLTTMSTLEFSVIYLKVNKIVIMGHTDCGAIKSCSDSNVYNNLSSNLHQYLKPINDLINEKRPELSELNEHEQMKQFSIYNIKKQYKTLTEIPLIKEYLENEKLEIMTLLYNVDTGLIEPVVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNG0</accession>
    <name>A0A1E5RNG0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Leucine aminopeptidase 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Leukotriene A-4 hydrolase homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g835</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNG0"/>
    <dbReference type="FunCoup" id="A0A1E5RNG0">
        <property type="interactions" value="1018"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNG0"/>
    <dbReference type="InParanoid" id="A0A1E5RNG0"/>
    <dbReference type="OrthoDB" id="79562at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004301">
        <property type="term" value="F:epoxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09599">
        <property type="entry name" value="M1_LTA4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.320">
        <property type="entry name" value="Peptidase M1, leukotriene A4 hydrolase/aminopeptidase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049980">
        <property type="entry name" value="LTA4H_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038502">
        <property type="entry name" value="M1_LTA-4_hydro/amino_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034015">
        <property type="entry name" value="M1_LTA4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015211">
        <property type="entry name" value="Peptidase_M1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45726">
        <property type="entry name" value="LEUKOTRIENE A-4 HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45726:SF3">
        <property type="entry name" value="LEUKOTRIENE A-4 HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09127">
        <property type="entry name" value="Leuk-A4-hydro_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01263">
        <property type="entry name" value="Leuk-A4-hydro_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M1 leukotriene A4 hydrolase/aminopeptidase C-terminal" evidence="12">
        <location>
            <begin position="579"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="406"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="495"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="165"/>
            <end position="167"/>
        </location>
        <ligand>
            <name>a peptide</name>
            <dbReference type="ChEBI" id="CHEBI:60466"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="358"/>
            <end position="363"/>
        </location>
        <ligand>
            <name>a peptide</name>
            <dbReference type="ChEBI" id="CHEBI:60466"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="428"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="692"/>
            <end position="694"/>
        </location>
        <ligand>
            <name>a peptide</name>
            <dbReference type="ChEBI" id="CHEBI:60466"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR634015-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR634015-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR634015-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ88432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="736" mass="83814" checksum="7103FEF302921814" modified="2017-01-18" version="1">MTNYLPPSIAKYHGLESIDPSTFSNYTEFDIKETFLDWTVLFDKQIICGSVTYTLKEKFGDQKNANKRTKRVVQSVHLDTSYLDISKIEVNGTQVLENECDGWYLKERCGDLGSELVIQSDVISDCLIKGEEFVVKVHYATTQKCTALQWLNGEQSGDTPYVFAQLEPIHARSLFPCFDTPGSKSVYKAVIKSEQPVCFSGLPIMSETNTSAANKSAANKSAANTSAANTSAANTSAANTSAANTSVASINEVNINDLKTSNSIESATSCIKLSSPFKEYCFVQKWPIPTYLLVIASGNIQTHSIGPRSSVYAQPHFLQNAINQFTPDMEKMIQAAEDILYTYPNEDYSIAIMPTSYPFGGCECMNCTILSPSILNSKFKNMDQSVTTTTKSQNETTEFNSTLAHELMHTYSGNLVTNSTWEHMWLNEGTCVLLERKVLAHLFQDERVRGFESLNGWNDLQNSIDNMDNPQRFSSLVHDYSDKINPDLGFSTVFYEKGAALHYTLEQLVGGAQYYDQFLKHYFRTFAFQSINTWQFLDCLYEFFYHYDKTKYKVLYDEMDWNTWLFQPGMPPKPKFDASLCEKVYNLTAKWVKCATTGGDPHKFFSSNDIEHFSVGQMILFLDALTNDKEYGMKNWFEFSNLVQVFFSLYDYKFQELKGYAETDFRLFKFKIHAHIKEAYKELSAWLGTIGRMKFVRPSYVLLKSVDKPLAIATFKKYKHTYHPICYSMVKQDLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNN1</accession>
    <name>A0A1E5RNN1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">60S acidic ribosomal protein P1-alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g952</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes). The 5 acidic ribosomal P-proteins form the stalk structure of the 60S subunit. They are organized as a pentameric complex in which uL10/P0 interacts with 2 heterodimers, P1A-P2B and P1B-P2A.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNN1"/>
    <dbReference type="FunCoup" id="A0A1E5RNN1">
        <property type="interactions" value="820"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNN1"/>
    <dbReference type="InParanoid" id="A0A1E5RNN1"/>
    <dbReference type="OrthoDB" id="2194681at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05831">
        <property type="entry name" value="Ribosomal_P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696:SF10">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="68"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00063629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="107" mass="10927" checksum="F27AC4F8D198C6F8" modified="2017-01-18" version="1">MSTETALSYAALILVDAEVEISTDNLLSLTKAANIEVEGIWADIFSKALESQNIKDLLVNFSAGAAPAGAAAGGAAAGGAAAAEEEAEEEEEAKEESDDDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNN8</accession>
    <name>A0A1E5RNN8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycogenin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g978</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNN8"/>
    <dbReference type="STRING" id="56408.A0A1E5RNN8"/>
    <dbReference type="InParanoid" id="A0A1E5RNN8"/>
    <dbReference type="OrthoDB" id="2014201at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050587">
        <property type="entry name" value="GNT1/Glycosyltrans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11183">
        <property type="entry name" value="GLYCOGENIN SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="396"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="439"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="495" mass="57353" checksum="2E5B5898DD8711D9" modified="2017-01-18" version="1">MKRKVAIVNLLYLDESHESDSKIFMEYYNSCVTQAKQVQMGIVAEQLKYLSFSDNGLSQQTYNIEQILILNQSAYTFLKQCPLSLRKTTASHHNSTNVFPQSDLFEYVKALYKERVVVLPNSNIIKYKLHCWSLKHYDMVLYMDLDMYFKEFANVLDIFTQYGIDSAVQLKPDQIVASTESTWCDMFNTGLFLIVPTQKKHAELVQFAQNTESIDTKDQGLLNQYFNPYFQDSPAYATKLTATNKESSTWVRLSYCFNVMMTPRIHSNGVTSGYDAGQTFLYSIEEGWWNKNGSVNQNFNASRAQNKKLFERFVDKTKIVHFIVKPWSIGIEYLKPKDNQEYNPNDFYIQWHRVYEQNVPLEIKRYPVKAIKICALKEFVQKCDIDTHKKTETQTLKKNTARNTHTQIENQPDKIQSTAQDDSSSRASRLPGKNRLHESPSTATHCKDNSSAPPIVQPPVSRLEKAVNVVETNAKDTLFPWCQYKDYPKPTRTFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNQ5</accession>
    <name>A0A1E5RNQ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">L8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">RP24</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">YL11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g802</name>
        <name evidence="8" type="ORF">AWRI3579_g889</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2016-08" db="EMBL/GenBank/DDBJ databases">
            <title>Genome sequences of representative isolates from three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNQ5"/>
    <dbReference type="FunCoup" id="A0A1E5RNQ5">
        <property type="interactions" value="936"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNQ5"/>
    <dbReference type="InParanoid" id="A0A1E5RNQ5"/>
    <dbReference type="OrthoDB" id="3969733at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000003">
        <property type="entry name" value="60S ribosomal protein L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000004">
        <property type="entry name" value="60S ribosomal protein L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="entry name" value="Ribosomal protein L6, alpha-beta domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_uL6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_uL6-like_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_uL6_a/b-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF16">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="60S/50S RIBOSOMAL PROTEIN L6/L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="Ribosomal protein L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 9" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="7">
        <location>
            <begin position="12"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="7">
        <location>
            <begin position="97"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00075840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00080918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00082770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ88318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ88525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="191" mass="21486" checksum="1296A054AF8C484B" modified="2017-01-18" version="1">MLYIQTEQSIEIPEGVTVQIKSRNVKVTGPRGVLTKSLKHIDVTFTKVNARQVNITVHSGDRKHVAALRTVKSLVANMITGVTKGYKYKMRYVYAHFPINVNVIEKDGEKYVEIRNYLGDKQVRLVKVREGVSVEFSTTQKDEMVLSGNSVENVSQNAADIQQICKARNKDIRKFLDGIYVSEKGVIDAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNT1</accession>
    <name>A0A1E5RNT1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CCT-beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g775</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin. In yeast may play a role in mitotic spindle formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNT1"/>
    <dbReference type="FunCoup" id="A0A1E5RNT1">
        <property type="interactions" value="1481"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNT1"/>
    <dbReference type="InParanoid" id="A0A1E5RNT1"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03336">
        <property type="entry name" value="TCP1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000002">
        <property type="entry name" value="T-complex protein 1 subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012716">
        <property type="entry name" value="Chap_CCT_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02341">
        <property type="entry name" value="chap_CCT_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ88540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="544" mass="59707" checksum="FAAB4D8BBEB9B6C0" modified="2017-01-18" version="1">MSINVFKDQVVEEKGETARLSSFVGAIAVGDLVKTTLGPKGMDKLLTSASTGASLVTNDGATILKSIPLDNPAAKVLVNISKVQDDEIGDGTTSVTVLGSELLREAEKLIDQNKIHPQTIIEGYRMACKAALESLQKIAISYEDFESDQHQFQIFKKELLKISNTTLSSKILSQDKQQFSEIAVNAILRLKNGTANKNSQEQHNVNLDNIQVLKIIGGKLSDSFLDEGFILNKSWGNNQPKVLENCKVLIANTTLDTDKIKIFGTKFKVDSTSKLAELEKAEKSKMKAKIEKISKFDINCFINRQLIYDYPEQLFTDLKINTIEHADFEGIERLALVLGGEVVSTFDNPELCKKGYCDKIEEIMIGEKQLLRFSGCHQNEACTIVLRGATEQVLDEAERSLHDALAVLSQTVNDSKTVYGGGCSEMIMSKAVDTVATNCEGKKQLAIESFAKALRQLPTILADNAGFDSSELVTKLRSAIYNGMFTSGLDLNNGSVGDMKELGIVESYKLKKAVVSSASEAAEVLLRVDNIIRAKPRTADRNNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNW3</accession>
    <name>A0A1E5RNW3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g746</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNW3"/>
    <dbReference type="InParanoid" id="A0A1E5RNW3"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="288"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="313"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="475"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="501"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="541"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="597"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="630"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="655"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="700"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="736"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="772"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="812"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="872"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="916"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="953"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="969"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="979"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1014"/>
            <end position="1025"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1056" mass="116728" checksum="54BCA525E53B45A8" modified="2017-01-18" version="1">MEGQLDNSVHPLLSLFEEQQFDLNEKWNIVDRIRLCKLILHCRSQNEGNLKSQLGDILNSLNNRADNNSQLKDRVFFLDNEEKINFSQGYVYSKEDLLQYLNNYGSLHRELEKNGKFSIEDFESKKTKDVLPIDNERTDTMDINVEKKEDTIQPKVRRLQLDDKCSNVSEIQNSRDHSSTAVNVSSGSQGAVQTIATENIQTVSKDIEGNNLKKAGKEGVTEDTDFNESSFKKVEPREEERGHANSDTDDKDGDIEMIDMTQKTAAEVSENTGSRPKDIAKAKQDETSALSSNNEQSSDSEDDLSEISRVESETNSSIRLSRSSSQQDDSEILQSSSSSEPRLPSITETKDENISHSLPNEDLSEAANEDLSEDANEDANEDANEDANEDANDVEKAALQKTLEADKFEENKDKEQEDISQATTIDSSNIEKQTNFLGQKNNVIKSNDSKNSESLQSNEVESLSGKKSNANVMETHTHDKVKDYSDETDNSHGQEAEENYLKSNKSGSASSIEQTPEQKTSASDDTVESEENTIKADNKVSDINVSQGLATSGDQLFDKKSTSKSAEIGSSDEDMMSDRSAADNKQPNEVGDEETKTTSSDVESSSGLNDPGESPKISKNILAQTQDQYLVDKEKTKNEEEYNTNSTAVKDDNTGRNGSQENYQKSTQSDTGIAADDEKTTASVENTPAAAAENIKLSASRKESISDKEHFESNHTENEEIISSEHDAVKPPVDFSKNEETLQKPDNIDDAPKLSKQAKDDSARLESLAAKPKPDNGETNAHLKTETQGDELTELRKDVVGGSEKQNTRGTTRGRGRGRGRGRGRGRGAGRGRPNARQNLERSDMNVEPQDEDSLDEQTKDGRAGSNNSEDGGQHTDEDESKEPTIETRTRSKIKIDDKAIENIVYGDVNKRHTKSSASKTRISKSPESNTSPSLSISDGSITGDKITKNAPNEDDIEIADETETPVPKRGRKRKSNRLSKDRDVDYSALENDKEVTSETDDGKASMATKRGRSKESSEVSQHADKKAKKSNANKSLSKSDTNDDEPIGRRLRNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNW6</accession>
    <name>A0A1E5RNW6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g742</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNW6"/>
    <dbReference type="InParanoid" id="A0A1E5RNW6"/>
    <dbReference type="OrthoDB" id="4036151at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="222" mass="25555" checksum="969EE91458C40C08" modified="2017-01-18" version="1">MSNRKDGLHDERMKTRTGSDQSSSSGPTGSVITTSGNSETAEKDTDIGTKNSTETSTATFIKEGISNVEIQNKLTKIEDDYATRTRIANKRRATKSLQVQLRKNAIKKQRLKEREIKKNNKFNRTSKAEKAYFEQIHQQQWQGEALEESEVREFEKLQQHAVREFEQLQQHAVRETKTSEQLQQSEVREPRLDESTKIGFIQTTTTGQVKNKNKKKKKIIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP08</accession>
    <name>A0A1E5RP08_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Signal recognition particle subunit SRP14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Signal recognition particle 14 kDa protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g931</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER).</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of a fungal signal recognition particle (SRP) complex that consists of a 7SL RNA molecule (scR1) and at least six protein subunits: SRP72, SRP68, SRP54, SEC65, SRP21 and SRP14.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SRP14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP08"/>
    <dbReference type="FunCoup" id="A0A1E5RP08">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP08"/>
    <dbReference type="InParanoid" id="A0A1E5RP08"/>
    <dbReference type="OrthoDB" id="19209at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030942">
        <property type="term" value="F:endoplasmic reticulum signal peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.10">
        <property type="entry name" value="Signal recognition particle alu RNA binding heterodimer, srp9/1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003210">
        <property type="entry name" value="Signal_recog_particle_SRP14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009018">
        <property type="entry name" value="Signal_recog_particle_SRP9/14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12013">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 14 KD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02290">
        <property type="entry name" value="SRP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54762">
        <property type="entry name" value="Signal recognition particle alu RNA binding heterodimer, SRP9/14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0733">Signal recognition particle</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="126"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ88606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="161" mass="17957" checksum="7CCFBC265BF2B280" modified="2017-01-18" version="1">MSSHLSNEAFLLNTTSLFAEANKLHRNVRVSMKRIIPQNEVEKPKELDSSAHPEYDVSKMSRSYLQIKPKSSKISQKRYNILIRAVMGAGRGGKDCTKMKISTIIHPDHLDHFWQTYSANVKAGMTGLSKKKKKKGTASVGASKAGKDDKVTKKSIKKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RPM4</accession>
    <name>A0A1E5RPM4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Vacuolar import and degradation protein 27</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g607</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RPM4"/>
    <dbReference type="FunCoup" id="A0A1E5RPM4">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RPM4"/>
    <dbReference type="OrthoDB" id="10251113at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040458">
        <property type="entry name" value="Vid27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013863">
        <property type="entry name" value="VID27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040768">
        <property type="entry name" value="Vid27_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31913">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31913:SF0">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08553">
        <property type="entry name" value="VID27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17747">
        <property type="entry name" value="VID27_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69304">
        <property type="entry name" value="Tricorn protease N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vid27 PH-like" evidence="3">
        <location>
            <begin position="205"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar import/degradation Vid27 C-terminal" evidence="2">
        <location>
            <begin position="365"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ88643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="702" mass="79652" checksum="F4132D819C105783" modified="2017-01-18" version="1">MEGLISSSVDDLSNDNCILKVIVNDSGKKLCVELPDAKRSLEFPVNSNFVLNFDMNNVLVFWTHRGIEYFIYPLRSDDAEFRETYDYFSSIIPCEEIFDQKKEDEEEEEEKEEEEVELTLSDIPALQAKPKNLAQKSLEKNEAAAEKLDFESKLKNLVNPSLKKKTPTVKSEQATLKNNENVDDLLFDVNKKMTALSLKSEATFENVQISVYDTVREKEFVQAKNGSVAFHFSKQSDTWLQLLNKKHESLANTDCNTDIDPEFDIFSLTFKFKYFLEEKVKITYIVTFHNLLQYTKFKAAYLGYQEANRTGNSPAQVSRNFGSFFNRSQEEHSDDSESEYEEASDEADQTSAIIDSFYRPGSTTRSLTVGLNSNRSYVVHDNNISVIKEDVINDELEYISTFKNVSYHDSKPFVPSNPMMYLQDQALIFQNENAPTNLYKMDLNKGVVVEEWSMGDNPVVNYSPAKKFDQLNLEQTFMGISKKSVFKVDPRISSKSKIVQDSNFEYKTNPNFSSIATSQSGLVSLGSKNGSVRLFDKLGGRAKTLINGFGDTIKHVVVSNDGRWLLATCSQNIILYDLLIAEGKPNAGVTGFRKSNSDPNFYFLTVPESFEEEQEDVSTKTPVEFSKAYFNTGIEVKETTIVSSYGFYVLSWKLSNILKGHLEPVVLKKFADNKVRENNFIFGSNDKVVIALNKGVTLASRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV23</accession>
    <name>A0A1E5RV23_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative ATP-dependent RNA helicase ECM32</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g407</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RV23">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV23"/>
    <dbReference type="InParanoid" id="A0A1E5RV23"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF317">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE ECM32-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6" id="KW-0347">Helicase</keyword>
    <keyword evidence="1 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="698"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="715"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="250"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="365"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="406"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="180"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="195"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="302"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="330"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="427"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ90797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1245" mass="141437" checksum="7BD9AEF702EB9B2D" modified="2017-01-18" version="1">MATKXNSSASIDLFXCQTCKETLLGRDMRKHVVETRHKCILYMGNDEIIQCEDCDNDNVHMLQLVRLGGYDLQCLCKNCLQKSNHAADISTVENVAYYDVAQNGQLFQYWKSYTDFSSIXCCKCGGEDNLNVSAKPNNSEVLCRECLKKNGKKTADYYADDSGQFLYKFLNISEPVKRKATKNSKFQRNKKVGKKQMERKEPTKQQLEDKRYQDIKAKNRIIQKSEVNLSQFKGVNTGEGKVASVLSSVLKGKKAPALKNGKNDKGRNAKGNQSPNTFGKKFGSAPIAPPSKSRTHTSSAPKXTGKSSVVSKNSNAKSSTAKGSKPKINEASNFKAQNFGTQPGKNGFKAEGKATPNTKIYVQKGDVQQDEQSAKLDGKNSKNPPGKRLKENRAQEKLVKDALKKGNQTNGSVGSIXKTTNNAKDSMTSKEEEKNSNAKVNVNKNSSEEGEDLFTNTKVDTKTKFNSIDEYLDYLSYFFFLEQLYELERDYLTRFDLEWGFQGESGEQMFKITLPFSQAPGGGENGSFNEYSWPDHYINMRRLPFNKNQVMILSRNLADDKSNFSNNYEKYKNNFWYCSIVDIKPILFKDLRKKNTRSREDNKYNKRRPKNGKAPKLVKKDVAGFEILLRVFPWCLNLGRTLPVKELYTNFKILPCVEQMNRVFGAMSKIENPDFKRLLIGRDNSVKDVHYKNNLSYFNSHLNESQFDSVQHSINNRVTIIQGPPGTGKTSTIVEIIKQLVLTLRNQPILVVAASNIAIDNIAEKLLEHGVENDNSSKIKVLRVLATSRESQYLKESKKPVEQAIFGLENLNINNKTAPFEENDAETKSSRDKVLDACLHNIISSRMSEQDRRMFLKFIRNDVSMGDYNEMTKMLDRRTKISDQIILQSQVILTTNVACGGKSLHVLDKIPYVIMDESTQSNEPFSLIPLSLPGIKRFIFVGDTNQLSSFSNIKEMRSSLFERIINNKMVESPKILNTQYRMNPKISKFPITNVYNNLLLDGVPDDIKKRLSPTTYPLYFIDYSNKNGIDSRNIDESKKTSLYNSSNDGHKKIKKISFMNECEGYLILDILQELIKSLKCDKEKITIITPYSGQREYLSELIRDNCYKWAENSDIINGNDIIRNSDKDELYQIEQNTSTSNTINIVDKISISSIDAYQGHENDVIIFSTVRSNPKNNNIGFLNDMRRLNVAITRARKSLIVLGNFNTLQKYGDTIWIKYCDFLKQAGVVFPGFSEYNNDFSNLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVF8</accession>
    <name>A0A1E5RVF8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Heat shock protein 42</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g285</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVF8">
        <property type="interactions" value="740"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVF8"/>
    <dbReference type="InParanoid" id="A0A1E5RVF8"/>
    <dbReference type="OrthoDB" id="5511210at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="310"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="28"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="147"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="370"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="59"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="147"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="197"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="210"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ90940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="490" mass="56067" checksum="14CABBE8EEF1B31F" modified="2017-01-18" version="1">MNSYTVDSTQDTLQSTLLDILKQLGVSESQIPLQQESQRAKSKTKTNSKSKNQKTGANPKHRKKHPKTKKSTSHSNYAPYPQFIPHQYMQYPYLVHPFQQPQQPQQPYPQPLLQPQHQAPFPFLIDTNSRRFGEHYPYYRNYYGDESEVETENEKDEDLQEPHFKFSNDYEDEDEGEDDEYYNDFLANRDDSEYDRDLRSLPLKHEKATTSKPRGSNKINVPSPFYFQPRYLDTPEFVNEDSLATKTIKGNENQSAVNDLAGGKNVSLIDNKKKPTHRNLGELMEDLLFHHSAAPSSPITDSLQVSSKSSDLNLPFSPLLNVYDFPNSYSVVVAVAGTTPQSLEIDFHPSSHELILKGSVSLPSLKDIAIEEDKKDTGNEKGXEEPIKDGNQEQQSEEKAKDNNTQEKQEVSKDDKSVPRCRIAELRTGHFQRIIKLPLFPKIDDENIKANYTNNGYLIVKLKKLSEHVDSVPKPKKTIRIEDIPDEDGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVG3</accession>
    <name>A0A1E5RVG3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 6</fullName>
            <shortName evidence="5">eIF-6</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TIF6</name>
        <name evidence="6" type="ORF">AWRI3579_g506</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer. Associates with the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleolus</location>
        </subcellularLocation>
        <text evidence="5">Shuttles between cytoplasm and nucleus/nucleolus.</text>
    </comment>
    <comment type="PTM">
        <text evidence="5">Phosphorylation at Ser-174 and Ser-175 promotes nuclear export.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the eIF-6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVG3"/>
    <dbReference type="FunCoup" id="A0A1E5RVG3">
        <property type="interactions" value="1128"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVG3"/>
    <dbReference type="InParanoid" id="A0A1E5RVG3"/>
    <dbReference type="OrthoDB" id="4155914at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00527">
        <property type="entry name" value="IF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.75.10.10:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.75.10.10">
        <property type="entry name" value="L-arginine/glycine Amidinotransferase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00032">
        <property type="entry name" value="eIF_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002769">
        <property type="entry name" value="eIF6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00323">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10784">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01912">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006413">
        <property type="entry name" value="IF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00654">
        <property type="entry name" value="eIF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55909">
        <property type="entry name" value="Pentein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5 6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="modified residue" description="Phosphoserine; by CK1" evidence="5">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine; by CK1" evidence="5">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ90815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="245" mass="26526" checksum="FBD6023944568F5F" modified="2017-01-18" version="1">MATRTQFENSNEVGVFSKLTNTYCLVTLGASENFYSAFEAELGDAIPIAHTTIAGTRIVGRMTCGNRRGLLVPTQTTDQELQHLRNSLPDSVKIQRIEERLSALGNVICCNDYVALVHPDIDRETEELISDVLGVEVFRQTISGNVLVGSYCALSNQGALVHPQTTVQDQEELSSLLQVPVVAGTVNRGSAVVGAGMVVNDYLAVTGLDTTAPELSVIESIFHLQDAQPESISKNLRDTLIETYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVR2</accession>
    <name>A0A1E5RVR2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Vacuolar protein sorting-associated protein 41</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g438</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVR2"/>
    <dbReference type="FunCoup" id="A0A1E5RVR2">
        <property type="interactions" value="706"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVR2"/>
    <dbReference type="InParanoid" id="A0A1E5RVR2"/>
    <dbReference type="OrthoDB" id="244107at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098588">
        <property type="term" value="C:bounding membrane of organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057780">
        <property type="entry name" value="Beta-prop_Vps41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045111">
        <property type="entry name" value="Vps41/Vps8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 41 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23411">
        <property type="entry name" value="Beta-prop_Vps41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23556">
        <property type="entry name" value="TPR_Vps41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vps41 beta-propeller" evidence="5">
        <location>
            <begin position="208"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="3">
        <location>
            <begin position="792"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="35"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="145"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ91027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1035" mass="117282" checksum="2B2F0DE2EC9566CB" modified="2017-01-18" version="1">MDIPDTIGGQVEHDSQSSQEKDKYTSVQSQPDNIVSREDAQDLKRESGTFTSQNSETFATEAAEDKASLNSSDHAENLQQTTDQLASKDLDTSDVHIPNSTNSTTSAKLQLPLAERQKLPGGSSLKKSPEDDLIDSGDSGSADTHEEEDEEEDEEEGEGTIDSEEDEEEGTIDSEEDEGEDTTDSEEDEGEDGDDDEDEEGESEPPALKYTRIKQFPEKLFQRDSISSCLFHESLFAFGTHSGVLHLMKPATFETIRSFKCHRASIMNITTQGTYFATASIDGTVIIGSLEDPTELMACDFSRPVNFVVLDPDYQASKKFVSGGMSGDVMLSSRNWLGNRVDTVLDKGHGPVVGIYIVNDILFWMNDRGITFISISSKTKLLNIPFPYKDKRPDLYWPSVHFPETDRIIISWYDHVWFFKVEILKDIDRTNGQTLGSILSSAASSLRALPDKKIEMEYHAQLQLRNEEVIVDISSFKDEQLIYLAASNAQCPELKIYDISLGEEQSSAEIPMRNYKSLNYNDYHMGKYLGNRGAANYYIVSSIEVIVAKELTLKDRFDWYMETKDYYKAWEIGFHLDPMDRLELGLKYIELLKKQEKYKDAIFATTKIFQKTIEGTIDDEQENEFFQLAVSKWTNILMSYLSLDLYDLVAANIPQGLGIDHALYDKILLHYLKCGHIEKFTSLLHTWPDGSFDISCIIDAMNNMERSRPLKKELAYLYHRNKDYLHSVDILLSLKDMQALDILLSHPIFANFTNKIHEIVLLPISSQAEPRDVATFSLKEIKEVFAKPLRLLVENRYSISLKSVIAQLEKDPQTCPVLYAILNRVSKKDPLLTANFEDAMISLFTKNFVPRDDSFDLLNFLKTHRNYSVDQAIVNCENSTTPKVKELIYLWSRIGEWEKALFILLDHSADDPKTAISFVKEHTVKAENMKRASELWDVLIEYSLDKPDFIKAILLCSDSSSDDCNSIDNKAKITLIERLSDSQQIPGLQNSITQLLINQYLASNVNGSVLSIVTDEASDVANKYLKQRTRGKMFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RW92</accession>
    <name>A0A1E5RW92_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sortilin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g488</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RW92"/>
    <dbReference type="InParanoid" id="A0A1E5RW92"/>
    <dbReference type="OrthoDB" id="4066654at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036278">
        <property type="entry name" value="Sialidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031778">
        <property type="entry name" value="Sortilin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15902">
        <property type="entry name" value="Sortilin-Vps10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50939">
        <property type="entry name" value="Sialidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Sortilin N-terminal" evidence="2">
        <location>
            <begin position="63"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ90973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="613" mass="69396" checksum="95894D52647DB8ED" modified="2017-01-18" version="1">MQKVIEKYGQKTPKNMMNKTXSPLVYELVTKEGDFEKNTDPTDTSIVAFTNSATLLFAGQKNRVIISNDNGLHWETVLKFETPKVNDPSNFLNKYIQLDAVYTDKRAFFFPQNETHNLRACCTNDIGRTWVTFNLQXAVEKHFYDFSVSKTPSQRASFVRLVRIRSSPINNKHLLASVSLNNDDKCNTFNIDFLSLDFGQSWVPVMPIKSIAFEKHIYSIKCFFGQKSDELFCLLLKEEPLSCQDKTNNEESPEDQKCTESGKLYYSTDLGNTWSLQEDLKNXSVVDIDIKGNFVVAYVPEKNIDVWTCTHIYISNDFGKSFRKPKYMGLSNDXSQNQNPYIXGADFTNDVMVLNTSKGKTWIXDYTSLQFFLPKECGTQKDEIFLVHNLCNQPHTLLXITHRVQARIASQGNFMQSFXIEDTKISFDDGKSWTAINLNPLHDSKKNGFKENFCDIDSYKFKATXXPALSSFAHEPLDIFTLAGYLVRKDPKVNGFPSVGCTXVTQNGGRTFDXSLDVPLHVSIGDSGNIFLGFNSTRNGSNNKEAKNKLYYSLNQGHSWDCLLLAGNVEVVDVFALEEDGSGFQFLVCTYTTSSSEDASYKKYFVVDFSTVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZA3</accession>
    <name>A0A1E5RZA3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein SIS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g123</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZA3"/>
    <dbReference type="FunCoup" id="A0A1E5RZA3">
        <property type="interactions" value="441"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZA3"/>
    <dbReference type="InParanoid" id="A0A1E5RZA3"/>
    <dbReference type="OrthoDB" id="550424at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006413">
        <property type="term" value="P:translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000002">
        <property type="entry name" value="Dnaj homolog subfamily b member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000013">
        <property type="entry name" value="DnaJ subfamily B member 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051339">
        <property type="entry name" value="DnaJ_subfamily_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078:SF553">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="6"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="152"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="152"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ92281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="364" mass="38297" checksum="9BB615E3CDA8AE04" modified="2017-01-18" version="1">MVKETKLYDLLGVSSSANENELKKAYRKQALKYHPDKPTGDAEKFKEISEAYEILNDSNKREIYDTYGLEAARGNGPQAGGFPGAGGAGGAGGAGGFPGGGFQSHSFSDQDAFNIFSQFFGGGGGGAGASSGFGGDFGGGFGGSSGFGGSSHGFGGGMPGGMPGGMPGGFGGMPSGAGGRSSGGGHARPEEKVVQVNLPVSLDDLYIGKKKAFKITRKGANGQPEKKQIEIQLKPGWKAGTKLTFKNEGDYNPATGGRSTMQFILQEKPHELFTREGDDLVYTLPVSFKESLLGIQTSIPTIDGRKLPFQKIQPIQPYEVSKYPGLGMPIKGDLNNRGNLLVKYKIDYPLSLTPEQKEVIKNNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZG4</accession>
    <name>A0A1E5RZG4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein DOM34 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g128</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the Dom34-Hbs1 complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway (PubMed:20890290). In the Dom34-Hbs1 complex, dom34 recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel. Following ribosome-binding, the Dom34-Hbs1 complex promotes the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the eukaryotic release factor 1 family. Pelota subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZG4">
        <property type="interactions" value="544"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZG4"/>
    <dbReference type="InParanoid" id="A0A1E5RZG4"/>
    <dbReference type="OrthoDB" id="10249111at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990533">
        <property type="term" value="C:Dom34-Hbs1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071025">
        <property type="term" value="P:RNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.60:FF:000004">
        <property type="entry name" value="Protein DOM34 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000008">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.870">
        <property type="entry name" value="Pelota, domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038069">
        <property type="entry name" value="Pelota/DOM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058547">
        <property type="entry name" value="Pelota_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004405">
        <property type="entry name" value="TF_pelota"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00111">
        <property type="entry name" value="pelota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853">
        <property type="entry name" value="PELOTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853:SF0">
        <property type="entry name" value="PROTEIN PELOTA HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26356">
        <property type="entry name" value="Pelota_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159065">
        <property type="entry name" value="Dom34/Pelota N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0469">Meiosis</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="11">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ92274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="385" mass="43727" checksum="C9EB33511B4E930E" modified="2017-01-18" version="1">MKLLKEEKNRTGGATLAILPQDKEDLFTIYNIVNTDDEVVFKRSVSNKEEGSKKKVTELVKLHMKIVSSEFEPENEFLRYKGITVADPEANRDVAVGKYFSFTIDFKFPFTLIKEDYNNFCRSSLHEACNMESRTDVGAVVLQEGIAHVCLLTPFSTSIKNKVEYSMPKKKRSTDIKKYDEKTAKFYKNIVEAMIRHFAFDSLKVXLLCSPGFWASTLYEKLIKYAEENKVSEVLANKSKIVVAHCSTGYLQGIDEVLKNPAYSNKLNEAKNCNEVQVMDEFLDHLNKDDFKAWYGAEEIFKAADLLAIKTLLITDTLLRSDDVEERKKYMRLVSGVEAQGGKVVVFSSMHSTGEELDKLTGLACILSYPIPDLDEDEDSTDNEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZL7</accession>
    <name>A0A1E5RZL7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SAGA-associated factor 29</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g153</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZL7"/>
    <dbReference type="InParanoid" id="A0A1E5RZL7"/>
    <dbReference type="OrthoDB" id="3971871at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20393">
        <property type="entry name" value="Tudor_SGF29_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037802">
        <property type="entry name" value="SGF29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010750">
        <property type="entry name" value="SGF29_tudor-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047288">
        <property type="entry name" value="Tudor_SGF29_rpt1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21539">
        <property type="entry name" value="SAGA-ASSOCIATED FACTOR 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21539:SF0">
        <property type="entry name" value="SAGA-ASSOCIATED FACTOR 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07039">
        <property type="entry name" value="SGF29_Tudor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51518">
        <property type="entry name" value="SGF29_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SGF29 C-terminal" evidence="2">
        <location>
            <begin position="265"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="231"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ92407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="408" mass="45091" checksum="3244960173D7789B" modified="2017-01-18" version="1">MVASNGVNEKWDTVISLLQDLFNANEVIPFDYHTAKDANEENASDAQSETTLINQRELQHKDNCSRGKRILQEIQINLDGLLKDLDKNFAVNIEKQNGGLKTGESEATGGKHTLVHDESDVAEGGRGGRKRRKLAETLDLQEFPASLGKRGRPSAQDYNNDKPEASMHQTPRSLXDEAKPFKNGSLGSEDATALSGRKRKQALGDAADLPVDTKTNNLLRKEESPDSVXHATXTAATATTTTTTTTTSSKTVQHGKSYYASEFNANEPVLLGSQVAYKPMKKNSGITDWFQCEVTKILNSEGTKFEIKDPEPDDFGNPGASYKCNWKEILLIPPHPNIASARKLKXYPVGTKVLAKYPETTTFYSALVIGKTQTRNGLPTCILRFDGEEEVDKETEVERFWVLPFPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZR6</accession>
    <name>A0A1E5RZR6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Nucleoporin NUP82</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g41</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZR6">
        <property type="interactions" value="153"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RZR6"/>
    <dbReference type="OrthoDB" id="341482at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037700">
        <property type="entry name" value="NUP88/NUP82"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13257:SF0">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13257">
        <property type="entry name" value="NUCLEOPORIN NUP84-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ92457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="781" mass="89442" checksum="B8800EE808F8072F" modified="2017-01-18" version="1">MSDIPAGLKQNGLGGPFTTHFVTTAPTANDKETQKCIVFDSLGFLKIVSTVSKDAKETEEIIFKFETDVVKSSILIEYNKKSLIAFLSTDKKTIQLLDMQQKNLNDLVSSDLGNLFPSPVKQLQWHPLSADRTLVLLLEDSTLLQYDILANKVFRMDSETFNFKSESYMLESHFNYRDVSQYDFLEDISSFTFXKSGLKLYIAAKEDNDIYCIYPFFPYFEKTVALEKELATELFNESYVMYEQYMEDPSCNVNTKKCLIKQLKFASKYLFLENHDSNEVGIGPNENSNFKSKVTIDIPFSYRSEYGIQGPFLISPYPDRLYNLRLDQITTLDVGNGNEFLVLVISDGSVLICFEDIATPMYWSSVDPETSNSLISSFNCSLVGLELRVILNGFXNVYDFGDQSSLRIINAIPHRLVFFINNKLSEACXXKEDVAIVLDTILWSHNLSKALKESDSLVNLDNLESGFQKVEKVTSCAFVNNESLVFTTRSSXQLEVLKLKTPAEVDFGDDNELSKKFVDKFISTNSXKTKEIKYKYTLSPSIKVLLKELQSFSVDLNEKNNKSLYGSVTQVLADHKSIPLKELNDGDEEVYGIIESLNEEMTERLYLMNNYGFKMHTILQKLDFEFKXQIATASDLKNLTERIQTKKAAQLERYESLLQRYNKFMEKTQGLKAKLDSGLEQVSGKYFNTAISNSEKEWFMSLRKSILQFNEMVLQQQRYTEQLEYLKSTLGEIKRESLEKENSDMDFTDLQKLLTLDKNIIENCNDQLVSAAKELETKLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZS0</accession>
    <name>A0A1E5RZS0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial Rho GTPase 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">GTPase EF-hand protein of mitochondria 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AWRI3579_g230</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the mitochondrial Rho GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZS0"/>
    <dbReference type="FunCoup" id="A0A1E5RZS0">
        <property type="interactions" value="813"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZS0"/>
    <dbReference type="InParanoid" id="A0A1E5RZS0"/>
    <dbReference type="OrthoDB" id="10020961at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01892">
        <property type="entry name" value="Miro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000011">
        <property type="entry name" value="Mitochondrial Rho GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000553">
        <property type="entry name" value="Mitochondrial Rho GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013566">
        <property type="entry name" value="EF_hand_assoc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013567">
        <property type="entry name" value="EF_hand_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021181">
        <property type="entry name" value="Miro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052266">
        <property type="entry name" value="Miro-EF-hand_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020860">
        <property type="entry name" value="MIRO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46819">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46819:SF1">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08355">
        <property type="entry name" value="EF_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08356">
        <property type="entry name" value="EF_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037488">
        <property type="entry name" value="Mt_Rho_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51423">
        <property type="entry name" value="MIRO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Miro" evidence="18">
        <location>
            <begin position="4"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="187"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="311"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Miro" evidence="18">
        <location>
            <begin position="428"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OEJ92472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="577" mass="66298" checksum="8DAB47F14A2DB7B1" modified="2017-01-18" version="1">MQNKHAIKVVVCGDEHVGKTTLIANFVKNQYIPHLQKRLTPVSIPREFSDNPGFPETTILIDTTLEDTYHLQESLETADVILLVYADDKSYDRISSHWFSMFRSLGLNLPVIVCRNKCDLLSSQDYALVDDEDFLPILNDFKEVGSCIKASCLDNHNVHRTFFLCQRSIMFPLAPLYDSRIQALKPSCASALKRIFILSDKDQDGFLNVHEIAQLQRKSFNKSINISEVEDLIQHIQELRIPNTCSRTRGLTKYGFLLLNTLYIEKGRYETVWGMLRAFHYTDTLEIDEHILFPNIGIHQNSTCSIELSPVGYRFLVDVFRRYDRDNDGGLDDYELKQLFKSCPEGIPQLWLENSFPNSTPINNDGHITLQGWLAQWTMTTFLDYKITTKYLVYFGFEQNAKNALHITKQRKMKKRSGKLYRAPVSDRKVFNCFVLGKPNSGKTALMESFLNKQIVDSGLYSPTIRPQIAVNSMELKGGKQFYLILQEFGGSEAAVVENEAKWSECDVVCLAYDSSDPNSFAYLVEMIDKYSFLKKIPMVFVGLKADLDRQQQRCHFQPDEFTDLLYLDHPLHVSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S032</accession>
    <name>A0A1E5S032_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Endoplasmic reticulum-Golgi intermediate compartment protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g218</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a role in transport between endoplasmic reticulum and Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ERGIC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5S032"/>
    <dbReference type="FunCoup" id="A0A1E5S032">
        <property type="interactions" value="735"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S032"/>
    <dbReference type="InParanoid" id="A0A1E5S032"/>
    <dbReference type="OrthoDB" id="270930at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045888">
        <property type="entry name" value="Erv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012936">
        <property type="entry name" value="Erv_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039542">
        <property type="entry name" value="Erv_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984:SF25">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07970">
        <property type="entry name" value="COPIIcoated_ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13850">
        <property type="entry name" value="ERGIC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="404"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter N-terminal" evidence="8">
        <location>
            <begin position="8"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter C-terminal" evidence="7">
        <location>
            <begin position="145"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ92333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="442" mass="50392" checksum="8D7F1E297A5DC54F" modified="2017-01-18" version="1">MSSSSKLLRLDAFSKTQEDVRIRTRSGGFITLLCITSVFFLLISDWKQFNTVSENPSLVIDRDRNKKLKVSMDITFPHMPCNLLALDVMDDSGNIQLDLTQSSFVREEIDSETLQPIDHDEEGNAGFVSAEEALKNLPADYCGPCYGSKDQTKNDELSQEEKVCCQDCDTVRRAYLDVGWAFHDGKDIEQCEREGYVERVKREINQGCRVQGSAFLNRIHGNIHFAPGKGFQHPTRGTHTHDLSLYESYEKLNFDHIIHDFSFGPVLMDYNTQTKNEEFVTSNPLIGVEMKEGNHFLQYSYFAKIVPTRYEDHTETTGIKYTISEDKFKNDDGIKLDDKDVGKALKSLETFQYSATMHSKPVFGGRDEDHPNTFHSRGGLPGIFISFEMSPLKVINRKEYRMTWTSFLLNAISSIGGVFAVATVLDRMTYRTMQYVRSKKDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0J6</accession>
    <name>A0A1E5R0J6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Delta(14)-sterol reductase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">C-14 sterol reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Sterol C14-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g4440</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP(+) = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18561"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17813"/>
            <dbReference type="ChEBI" id="CHEBI:18364"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.70"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:18563"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="15">Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 2/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the ERG4/ERG24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0J6"/>
    <dbReference type="FunCoup" id="A0A1E5R0J6">
        <property type="interactions" value="484"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0J6"/>
    <dbReference type="InParanoid" id="A0A1E5R0J6"/>
    <dbReference type="OrthoDB" id="10262235at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050613">
        <property type="term" value="F:Delta14-sterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1630:FF:000009">
        <property type="entry name" value="C-14 sterol reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001171">
        <property type="entry name" value="ERG24_DHCR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018083">
        <property type="entry name" value="Sterol_reductase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257">
        <property type="entry name" value="DELTA(14)-STEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257:SF52">
        <property type="entry name" value="DELTA(14)-STEROL REDUCTASE TM7SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01222">
        <property type="entry name" value="ERG4_ERG24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01018">
        <property type="entry name" value="STEROL_REDUCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 16" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10 16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="8 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="13 16" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="9 16" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="12 16" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="319"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="390"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00060638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU369120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ80416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="443" mass="51421" checksum="A8643970405F4179" modified="2017-01-18" version="1">MSTKEKRIALNPKTTAFEFNGVSGALAITFGLPAVVVVLNQMIRPDYYIKGVFENFHIEEIVYNLRPLKDYFKLHEIWCYYLVWFFGLAFFDVVLPGKSMKGTELRDGSKLDYKINGIALSSLLLAILSFRWKLTDGQMPELQYLYNNHLDICIITILFSFLLATVVFIMSYIPLIFSKNKLGSKEKILALGGNSSNFIYNWFIGRELNPRIGPLDIKLFCELRPGMLLWLLINLSCLHHHYLKTGEVNDALLLVNILQGFYIFDGVLNEEGCLTMMDITTDGFGYMLAFGDLSLVPFTYSLQARYLSCSPITLGREKLIFIGSVMALGYWIFHASNMQKSEFRQGKLPNLKSIQTKRGTKLLCDGWWSVSQHINYFGDWLISLSWCLTTWFQTPLTYYYSLYFATLLLHRQKRDEHKCREKYGKSWEQYEKEVPYKIIPYVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0M2</accession>
    <name>A0A1E5R0M2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">separase</fullName>
            <ecNumber evidence="2">3.4.22.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g4624</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>All bonds known to be hydrolyzed by this endopeptidase have arginine in P1 and an acidic residue in P4. P6 is often occupied by an acidic residue or by a hydroxy-amino-acid residue, the phosphorylation of which enhances cleavage.</text>
            <dbReference type="EC" id="3.4.22.49"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.49" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0M2">
        <property type="interactions" value="309"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0M2"/>
    <dbReference type="InParanoid" id="A0A1E5R0M2"/>
    <dbReference type="OrthoDB" id="10255632at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051307">
        <property type="term" value="P:meiotic chromosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005314">
        <property type="entry name" value="Peptidase_C50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030397">
        <property type="entry name" value="SEPARIN_core_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12792">
        <property type="entry name" value="EXTRA SPINDLE POLES 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12792:SF0">
        <property type="entry name" value="SEPARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03568">
        <property type="entry name" value="Separin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51700">
        <property type="entry name" value="SEPARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase C50" evidence="5">
        <location>
            <begin position="1223"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="6">
        <location>
            <position position="845"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ80448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1402" mass="161275" checksum="D302D8E04BB2C710" modified="2017-01-18" version="1">MNVEPSELGLRSETLTENTVKKRSVVSLTDSTISEASHTTKKSKYCSAKVNHGLLFRPQELSYDTLHKSLSSSLLSAPNQQLFNTAEVRNGFENLYKYCIKHHLVQTLPTVISKHLNYVLSLLKCYSNYADVIGDEILYLHNITNHNECKNVIAILQTDFTVYNAQYLGSIKMLLLQYLLKSPAEEKYKMPLVTFFSKDQRFLYGGQVSTIKPMSILKVLLHLFTKYPDLKPLFGMKILQYQSEFGIXFETLVKSSTLVKFEKVLVXTASXLESKYHEFIHVYFAQCPLTSHVKKSEMYXPXSSSKTFVINERWLAKVEKSFSHTDNLKIYQLYYHYLHYLYSYSIPFIKEHSTLLDQILLTINTNLKSFYANPSLLNSVKATIYYMGEYLFDHKQYKKLANVINVAFNLYVTHNTDDLLKLAANLQMKLYYKLKEKDIWPKFKKFIKCSFSMELKFTIFSQIYNIFAIMASSELQKCCEFVFDGCVELSNLLPGLQLEELDFTSSLMNCLLYSQTAKKKHQIPIESWPVLVQMLYQTLDPYVELXAFDVNTQINKRECLHDNQVLIKCMYSLYLEMKKRRCVNLKTITQSYIEKRVKKNSFEESVIENLFVLNLASYLNFNNNYELVKYLYESIPQSYVKTHIEKFFIEAESQLTGRYDKQHVSIATFDAXMNLLDRLTQYEIILQQALATHDTKFFQTTFSVQLPFQDPXVFDIRNKGGEFSNKEYTSIILFNASLFLTAGKLSSHDNKHVNAFHSFVKALSLSNSLIKKSXKLXQNARVQLLNLIVACYDCLLEECVFMNLTSQFEYFVKEYISLSQVITDPVIQFEAYYSARFFAKLTNDDGYVAMTKKDMHLLDMDQNLVAFGKLGYIDSNVSLLLKKYEQAHYFLLTKWLIKSGIYLESISTSANLLNKPEVTDIDYKKLSMIDLRQYWMKREYHYKIADYVKSCSGFFEKQCSFNDKDFSPTLLMSSMTAHNPDIDAHTYPTGYNIVSLDICFESSKLIVSKFLNGSQSHFSLAVTRRNAKYNSFTALINAFKDIVDLSNGTTAAEVTSCVKTKEDRKKWWETRFELDSEMGNLLKYIEEEVLGAFVNVFAPYYIDHNEPEFQKFELEAKALGIDFDMQHLSLLLNQKNTKDLIEHGVIDEHDLALLVTLLEKHAAQLELCKKPVTHTVLITNNQAHSIPWESMPILEAISVSRMPSLESLHVLLERQEQSLQITKNDIAMIINPNGDLPKTQSRFEHLINGCESVMVNEKPTCTQFITMLKDHRAFVYIGHGSGDQYVRVSEIKKSADISASFLLGCSSAYMKPRGQMGSTGTIIGYLLGGAPFVVGNLWDVTDKDIDMFSLSMFEKMGFSNTKSNERQNVAAAVSMSRKVCKLRYLNGAAPIVYGLPIEIKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0M3</accession>
    <name>A0A1E5R0M3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4400</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0M3"/>
    <dbReference type="FunCoup" id="A0A1E5R0M3">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R0M3"/>
    <dbReference type="OrthoDB" id="3026777at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507:SF1">
        <property type="entry name" value="FI18259P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507">
        <property type="entry name" value="ZGC:174356"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="275"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="417"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="457"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="499"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="527"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="561"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="596"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="73"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="636"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="83"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="639"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="653"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="694"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="718"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="727" mass="81516" checksum="7AC73B5D6B43774B" modified="2017-01-18" version="1">MSQSTSQNGQFGFQPSNQQDDTAPTPTPEVLTNEGFAYVAEHTLQTILSQPAREQQAVNLDDGSNAFEQRFLVDDTVGPTDTLLPSTPVNNEEMQDTSFQRKPAVEEFTSTAIDSSDEDEQEDQREVEGEEAEDEDEISTKWYRDTVDMYQNKKWYQRPTAVALFVALTLKALSFTIMITPTAALTMEKICDSMRNDEGHCNMTTVQKEQSVVQSAVSLLTSCLGLVTAGKYGELSDRRGRVFVLQINGVITLVHVLLALYVFAPGTGYNRYGLIFVNSIESISGGIMVLISTGNAYISDIVPVNQRMVSISVLMSIVYGSLGLGPLLSSFTVKLFNGNNFVPFYLAVVCCLLFNAMVFYFIIESRHHDARRYSQSEQFMKEQIHGSSVYRKLFEVVEPLKHLWMPKTYSGSLIPRINVILLMILDVIFVMATQGAAPTLVLYGMLRFHWGAVELGYYLSISGLGRAVVLLLLVPQVVSMLKKSSIFHYKPLKTSMDRLDLVVLQWSVFCIFCSIAVAVLIQSRPSGLYLSAAIQTLSAVISPTIQSVVVKYASKKITGQVFGAIALFRHIVMLIVPLIFLRIYSETVDKNPAIFLYVPLVGSIIGFVLTFFLKIVEDPLILRRPSEITFLNERELTPQQKQQQEQQEQQEQQEQHFMERRKPSISSGSNPPKIKRSESIHSLEMGQGFVYDTADAHENASTKNTSDQKNKRNYSSIMERRPSVLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0Q7</accession>
    <name>A0A1E5R0Q7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">[PSI+] inducibility protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g4568</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0Q7">
        <property type="interactions" value="151"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0Q7"/>
    <dbReference type="InParanoid" id="A0A1E5R0Q7"/>
    <dbReference type="OrthoDB" id="6250593at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903078">
        <property type="term" value="P:positive regulation of protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039688">
        <property type="entry name" value="STAC1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15135">
        <property type="entry name" value="STAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15135:SF7">
        <property type="entry name" value="STAC-LIKE, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SH3" evidence="11">
        <location>
            <begin position="44"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="101"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="146"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="167"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ80484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="211" mass="23212" checksum="9FA7B6E51FAABEC3" modified="2017-01-18" version="1">MSAQQSVDTIKGELRKLKEHGCINDQLNSQFDTLISQINTNPAPALEYVEAIYAFQAQQDGDLSLQVGDKIQVLEKISSDWYKGKCGDRVGVFPSNYVKPAFDEQRGAPPVPAPPQYQQAIAPQNTSSSNXTTQSYQQPPFPPPSTGYYQNQQQYYQQPQQXPPPQQQQQLQQQPQKQGNGRLSKIGGQLGNAFVFGAGATLGGDLVNSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1D5</accession>
    <name>A0A1E5R1D5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1D5"/>
    <dbReference type="STRING" id="56408.A0A1E5R1D5"/>
    <dbReference type="InParanoid" id="A0A1E5R1D5"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184557" evidence="2">
        <location>
            <begin position="20"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="650"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="681"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="872"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="650"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="684"/>
            <end position="700"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1201" mass="126821" checksum="0C83087E203DAC66" modified="2017-01-18" version="1">MLIITLFLVVLQILHAVRAQDPLNVTFFNYSIAPEDPSSKLPTAGWLFSTSISINDPSSITTDSWFGVNMYGVYNVIRNFNQESFDISLPDGSPIFYCTPYNAYTNQGFGDVPFTYLECDSYVNVDVPNLGPLVADLQLIITFIAGNDFNYAYEANFGNPIDVSNDFLFGQIDASGDLTVQNFTIPVTFDTVADGSSEISYIRMGQDQVQEIYYVFPECQTGEMIDAQMTFIGDEDLQMNSWAYIGYGNTEVLNSWYLLKGTSFSSIQYRMTSSASVTGDSTATKTTFFMDYTDIAAGNRPLFGLEGTIIQTTSLFTNTLIGAFTCAYGTTSSVSQIAEASVVNLVYRDQIATIRDDTTITITKTSTWSSTFTSTATTIVPNPNYYNTLTVTVQVDVPKPTPTPTPTTTTTTTATWLSTYNYTSTEPVNSSGGNVTVTVDVDLAKPRETTTRTTTWVMGYATTTTEATNESGETITVTVDVDVPKSTTTTTTTATWLSTYNYTSTEPVNSSGGNVTVTVDVDLAKPRETTTRTTTWDMGYATTTTEATNESGETITVTVDVDVPSKSSKLPSFSTQSINSSTTGFEITKSGMSSISQISTASTCSTVSITATSMSASDSMFTVTTPSASSVLSSVTFSALKSSVSSVNSASNVSTSNFATSSMTKSSDVTNSTTALSSSIVMESKSSTNPSATKPSSATASLSQTASSSYSDIVPSNETSSIFKTTLANATLISWSSILTTSASSASSVDNLKNVTTTSRVATSSSNKISGSSTAISLSSISASENALTSSISRSFANSHNSPSSNYTISNANSTSSIMSSTSDKIILSSQTAMPTRSSFSSASLITTSFAPGADQSTFYQSLTTQPTFKYSNISSSSSSSSSSSTVPSTATSSLWTPQSIIVKVSSDSSTSTESVSLHTYKSTSATRPLSFKASTSPSMPTKPIVSSFSSATSTRSSALSSVLPNKLGTSSLSSSTGEMKTSLASVSASSKSTFLTTDVLSFTCSNVSSLSASSTVPISLNVQTSTEYTKSSSLTSLMQSASANASITSTPLNPKSSFAGATDSSVTGYSDTDTSLLEPKTQVSSSFQKYAYSSLSNIYSSPDLTFSTTVPSKHTSSESSKQVSLAISTMLLRTTTPISTEHATFSNENTTSSYFSIKSSHSASLTLININSDSGAACVKMSSHLTTLFTALTTIGMFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1E4</accession>
    <name>A0A1E5R1E4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">LYC1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4536</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1E4">
        <property type="interactions" value="106"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1E4"/>
    <dbReference type="InParanoid" id="A0A1E5R1E4"/>
    <dbReference type="OrthoDB" id="2020070at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055100">
        <property type="entry name" value="GNAT_LYC1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053013">
        <property type="entry name" value="LAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34815">
        <property type="entry name" value="LYSINE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34815:SF2">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22998">
        <property type="entry name" value="GNAT_LYC1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LYC1 C-terminal" evidence="1">
        <location>
            <begin position="219"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="419" mass="48163" checksum="185623C987C88A94" modified="2017-01-18" version="1">MSSAQIPENLVLVDCSNDMEMRGYCHSQNSATWKSDLLTVDEYVQREFALSTADLCIQHLKTSENSDDVDTGKEESQGIFRKYLGVRYFALKDMSLPETSNXSQIVSSVETLNKVGYVVPPESGKQVPVLSVCIGGVFTAEKFRGNGYAKSMIQLLNKHYDDMAKTPEGVKTGKFLEWKVMTLYSEVGEFYSKVEYVSDHVPVHHINKYNELLKAIEQKEGKNTKNKSVKYMNFKGYDELVDLEIKETEEKVAKAVEKRNAKKGTDFIFVLKPDILIYQWFAVRDIYIGCIAFPQLMAKNPLTYGVSVNNMSTKTNAHCMWHHAWAEKNLTILKVFTQRQDDIFELFKYMILEAQKYNLKEIHIWDEELSKEKYPNFFATLESVVDADHLSMKNTSLSAIRAPFHGKVEWLNNGKFCWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1M7</accession>
    <name>A0A1E5R1M7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Sporulation-specific protein 75</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3876</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1M7">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R1M7"/>
    <dbReference type="OrthoDB" id="1076608at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF20">
        <property type="entry name" value="SPORULATION-SPECIFIC PROTEIN 75"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="256"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="618"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="663"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="706"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="773"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="810"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="833"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="874"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="901"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="9">
        <location>
            <begin position="229"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="8">
        <location>
            <begin position="612"/>
            <end position="893"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1013" mass="117721" checksum="B6CBF39332471F6D" modified="2017-01-18" version="1">MWDDLMRPLKTRQSYSKHSKMNNHXSVVNDSYSQPLHLLSHKYFRNYHRMNASSFYNVTHSSSPHFINSTLFKQPYNRSIQEQPNMFSYYEDFLLSSFLNTTRSGSAHRHFGQSFNQFLGTVLLTVAFAFLQLFIFFVLKEKLKLFYQPNVVYSQLTRNDTGNSASGSYRSSKALQTCMKKENPGNGTKNVWKILYDSCAAEKILKFIAFVSQLLHCSVFQEHFNLFTQNVRWFINLAFHTSIDEYQHIGLDSFCILQFLTVLSQMFLLFSVVIIPILIPIHFYSAEQNNMQLEENRDSNAETSLIVLRKHGLDRISMSSISNKNSNVLVVHFVVTICVVIFFHFVLLKELQQYCARRMDFLHWLQNNHTTSKYFSNLWNAQRTLYFNNPSSLNPQILNTVLQRLGNPRIASISYVPRGYEKLLQYQKTHSKLLKRIESLELDLLVTKTYTSQWHHLSPTRTTRNLIREKLFTLKKIITFQTFVRVRIVRRSKAFSIVSKTQTFNYYKPKFTVHDDISWQTQNVNLLVGKYLQNLQEWLYYKNSLTRKTKNGDNLYNMVFIKFDNIYIANFFKQILIHGNPSICTKKCSNVSYKDIIWANVTRESPQVNFARQCIANFLSTAILIGWVMPVAFIGLMSQIPYMGKIVPFFRSMYKVPSSFRGVIDILVPVLTLIFLTEVVPIFFRWLSRMKGLIKHSDVELNVQQWYYIFTFLHIFIVVTVSSGISAIMKQVIKNPVTIPNLLASNFPKCSNFFCAFMIIKGLSYFSGNLLQPGRLFFLMLGKVCNLFSSRDTPREKYLSITKKTLTYNWGSVYPIFSLLASIAIIYCVIQPIILLISYVAFALILVSFKYTLKYQVSKKSNTRETLGAFYPRALFQIYGGVYCLELFMIGLFILSKCYRLSVLTVFLXIFSIVAHVKIKARFKSLLVNIPLSKYNGYNASPDTAIKSSSINGQYKFVSKMEKSGTHDIIWIPKDKYGMSNLEIRRLNMKFGETLKTSNSHCIIDHWGNIDLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1R8</accession>
    <name>A0A1E5R1R8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">asparaginase</fullName>
            <ecNumber evidence="2">3.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3982</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-asparagine + H2O = L-aspartate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21016"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="EC" id="3.5.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the asparaginase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.1" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1R8">
        <property type="interactions" value="68"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1R8"/>
    <dbReference type="InParanoid" id="A0A1E5R1R8"/>
    <dbReference type="OrthoDB" id="542841at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006530">
        <property type="term" value="P:L-asparagine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08964">
        <property type="entry name" value="L-asparaginase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1170:FF:000001">
        <property type="entry name" value="L-asparaginase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="entry name" value="L-asparaginase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004550">
        <property type="entry name" value="AsnASE_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020827">
        <property type="entry name" value="Asparaginase/glutaminase_AS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027475">
        <property type="entry name" value="Asparaginase/glutaminase_AS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00520">
        <property type="entry name" value="asnASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707:SF28">
        <property type="entry name" value="60 KDA LYSOPHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500176">
        <property type="entry name" value="L_ASNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00139">
        <property type="entry name" value="ASNGLNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00144">
        <property type="entry name" value="ASN_GLN_ASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00917">
        <property type="entry name" value="ASN_GLN_ASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="L-asparaginase N-terminal" evidence="10">
        <location>
            <begin position="73"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase/glutaminase C-terminal" evidence="11">
        <location>
            <begin position="290"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="active site" description="O-isoaspartyl threonine intermediate" evidence="5">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="159"/>
            <end position="160"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001220-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001220-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ80840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="400" mass="43804" checksum="EA5FD98099C50BDA" modified="2017-01-18" version="1">MKNQISTQTVGQMEITTICDDIKKSQFILSPSGSRNEELGGENELNTVDSNISFRSANGSRHFLHSETKKLPKIKILGTGGTIASSGSSASQTAGYKVDLTVQDLLKSIPDISGICEIAYEQLCNVDSKEMNEEILWLIYKTISQDIQKYDGLIVTHGTDTLSETAFFVENTIDSGNVPIVFVGSMRPSSSLGFDGTMNLYQAICIASSEKSKGRGTLVSLNDQISAGFYITKTNANSLDSFNVRQGYLGNFVNNEIHYYYPPCKPLGAGRFKLNLDLNGEPPKSNVFSRVAILYAHQSFNSELIELIVPHYDGLVIATMGAGSLPEALDNYLSXLEIPVIYSKRSMDGMVPIVNVPNSINCIASGYLNPEKSRILLQLCLYSQYSLKEIRESFNGVYGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1Y6</accession>
    <name>A0A1E5R1Y6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YMC020W-like alpha/beta hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4178</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1Y6">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R1Y6"/>
    <dbReference type="OrthoDB" id="5598028at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058934">
        <property type="entry name" value="YMC020W-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058933">
        <property type="entry name" value="YMC020W-like_ab_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47349:SF1">
        <property type="entry name" value="AER328WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47349">
        <property type="entry name" value="CHROMOSOME 8, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26147">
        <property type="entry name" value="AB_HYDROLASE_YMC0-YMC35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YMC020W-like alpha/beta hydrolase" evidence="2">
        <location>
            <begin position="385"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="268"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="808" mass="90417" checksum="D8D50F9281BF0C0D" modified="2017-01-18" version="1">MSSVASLNSQNKTNQNNFAKNNTNDTASSGKQNDLGQSEQAQKQDQEPEQRKXNTGLVKARQGSIVSSFWNIMQSAGMVSGSAPGAAIDNSKSLSDADLRAERNSESGVQPFSNFAEQDVETTREQGVEPELQREHVNTETLLAKPEDSNEQIIIETPAENVCKATISAKSIKPQTDMDRPPTSESTGSYNKLSWWXRKLSFAATSGEIDANVKQKSVTSIPQLSTEPPLKHPLETNGAESKEQKDKNAGTTTEPLDDALHQQLLKTESLNPDLDVTSSNKNSVDSGNRRTGDPSASMPVEVSNRLILHTEGNDFYERLKAASMDIDSDSADAKVDLTNSVDNYNDLATLEEQHNIVVPQFETLPESSILRNIYNAFHTTSSNIYTNAGDYQHLYRRKLPGKPKKILLIGVHGFFPNKKVRKLLGNPTGTSNKFIKEAEFAIRQYYHNKGLFGHKKSSDTQSQEVDLNEESPGSSLHISKIALEKEGMIFDRVEFFYEILTRYKKELNEADTVYFVSHSQGCPVSILLLSRLIEEGILLPPTKKRKIGVLGMAGINNGPSYGMEKKWVAKIFFSFEGDPLKELFEFQDFNSVQSKKYMAALKLIISCNVKICFVGSITDQLVPLYSSTCCFAFHPNIFRSTYIDKDSDTPQFLIKVVEAANRLTNMGMSDHNMIKEISRYLIGPLTGGGHSKIYFDQGVYLLGYQFMFETTDLPSNEVELNFDHHTGETVESLVCKTPVAFKPYKVSELGVEPTRFPWCMRGFLCEVEKHLGKKTIREMFQDFDQWDPKDEELVKMKASISCMLHNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1Y9</accession>
    <name>A0A1E5R1Y9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NADPH-dependent conjugated polyketone reductase C1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4375</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1Y9"/>
    <dbReference type="InParanoid" id="A0A1E5R1Y9"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016652">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19120">
        <property type="entry name" value="AKR_AKR3C2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000002">
        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044494">
        <property type="entry name" value="AKR3C2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF3">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="4">
        <location>
            <begin position="76"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ80902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="378" mass="42481" checksum="2200293FB7E9E2A6" modified="2017-01-18" version="1">MQRDSNLIGYAACIXSQSVPTFPLFVELFDELKSSKQNQNQSPFFYCCXMKQNIPTFTLKSGDKLPAIGFGAGTKWRIAKAEGSLDFIQELADQVSCAIRAGFTMIDCAEAYKTHLEVGXGLRDAGITKANRNTVWITDKYTTHSFAWRKGTGPVQSLXLALEKMQLEYVDLYLLHTPTINLESAGITLEDAWKQCEELQRQGLAKNIGVSNFGVSDLEKIYKICTIEPAVNQIEFHAYLQQQSPGIRSFCKDKNIQLQAYSPLTPITKAASGPLDPYLNELAQKYGKTCMQILLRWVIQNGVVALTTSGKEERIQESLDIFDFELSPKEIEKINKIGQTFFHRGWMERFLGHFGKEECNAGLENAAFLRSTLXTLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1Z6</accession>
    <name>A0A1E5R1Z6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 2, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4283</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1Z6">
        <property type="interactions" value="111"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1Z6"/>
    <dbReference type="InParanoid" id="A0A1E5R1Z6"/>
    <dbReference type="OrthoDB" id="416093at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF589">
        <property type="entry name" value="[PYRUVATE DEHYDROGENASE [ACETYL-TRANSFERRING]]-PHOSPHATASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="62"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="479" mass="54750" checksum="E22C2C0F529FA24E" modified="2017-01-18" version="1">MGISQKFIEWVPHNIPNGIAKLRLNLYKFPNYIGHASSRISRFQNEDNYSMAMLKLMSRNKEYQIDCMNKPSYTGQKVLKDKTEKDKHMFTKPVLNLNIFDGHGGKETSGWLSDNLATELARTIPNKETFFQILKQYTEQFPDSRYWKHIYEKKENYYNRFIVNCSTKKEQVLYNGSRMIFDKYGNIIDKTSLLSELDRLRIFQTFLGSDLQNCKNGLPGGSTASSIMLSPYNEDEKLPSATRDTFFVSSDHLMKLCVVQVGDCKVLICDKNGIAHSLTMMHHPNTTQEHKRLSHKDPALPIAKKPTETEQKKDENFFEDDENVGTDSFGEKRFLNVYANTRSFGDVQGKSMGLSCEPDIYSYLIGSTRNLPHQEKAKLXFGGDECFVCLVSDGVTNVMSDQEIVDLITSTVNNRGLKKATTQFVCDEVIKYLLAVSGKSADNATCLVLRLSNWGNWPVLDRTGAMREERLMNQDELRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R214</accession>
    <name>A0A1E5R214_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein ZRG17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4287</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R214">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R214"/>
    <dbReference type="InParanoid" id="A0A1E5R214"/>
    <dbReference type="OrthoDB" id="3973383at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="479"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="518"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="170"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="7">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="692" mass="78287" checksum="98744D4873ADF297" modified="2017-01-18" version="1">MQSTQDHAHSHDHTHSHDINSAQNSPEHTHQHTXNHKQTSNYDGHXHDQDDDHDTDGNGSMFQRPKSPFWDQPPSLSLNQNYVEPDNKNNLFKLPATPQSNHNFMESIYESDKEENDNIKYQPPPSGGNKSKRASIIFQNSPQQPPAISSFYKQGNNSSGNLISNPGFTFGGASGSNNDGLSDYDFMPPPPPPVLEAEGTGGSLNGSGPSSRRNSRYLPGPKLPPSSRVNKSPTRRHYSPERLPIITNSPKLIPRDGKPGNESPFSFAPLNLNSGSPSLNGNGTNSSVVGRAGFRKGHRYKHSSVSMNFFQEPEVKIPLNIAKALPIPDYNDVKSNLIWPQSHLKIAFTALELLAWIVCFQVGQXYHWNNFTTLSHFLFFDVLGATVLILVEILQQFEVWKQGTITFPFGINRVEVILSFAQAVSLCYMGLDLLFHIVEEGIVQFIEATSDHGDVSHDDIDSKIPHSHETSVLRQYYHIWYITVLFCLLFSTANYYYTYQNKNWTTKSINKLKNTNPLITMLYICYLMVYPLFPSVYLDYMATLCLGFFIIRYGAQIVKYSSNILLLGFPSQEYKIDTFKLMDQITSDIKDLRDYNPKTCEFKSCKIFKVNFNTYVALIRIQMYGGSNEREKLLRKSIDKYLRQRLCLTGTAPTQIDYETDESKDGTLKYAETHNSGGTPYVLESTIEIDLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R228</accession>
    <name>A0A1E5R228_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein arginine N-methyltransferase 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Protein-arginine N5-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Type IV protein arginine N-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4141</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R228">
        <property type="interactions" value="414"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R228"/>
    <dbReference type="InParanoid" id="A0A1E5R228"/>
    <dbReference type="OrthoDB" id="19014at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019702">
        <property type="term" value="F:protein arginine N5-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000310">
        <property type="entry name" value="Arginine N-methyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017408">
        <property type="entry name" value="Arginine_N-MeTrfase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051038">
        <property type="entry name" value="RMT2/GAMT_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026480">
        <property type="entry name" value="RMT2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32379">
        <property type="entry name" value="GUANIDINOACETATE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32379:SF1">
        <property type="entry name" value="GUANIDINOACETATE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038148">
        <property type="entry name" value="Arginine_N-mtfrase-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51559">
        <property type="entry name" value="SAM_RMT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RMT2" evidence="12">
        <location>
            <begin position="248"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="218"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ80950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="492" mass="56459" checksum="A054950EDEA11A05" modified="2017-01-18" version="1">MSDFHQLLTLSQRPITEDNLQKPVLEYLQQGIPATYTLEDAILERFGVVLVSSKGRNKSLSGSTTPLHILCRSVYYWNKGYDLQEESWLKDGLVNLDLSVKENMENTDNERMQCTGAPFALKKDQSKTDPLSEQEYDVVLQVMDLLFEYGAGWNFLDFEGQTIGELLLGEDGEESQLHDIQNQGLKRVYQRCVTAGVQAELLLRKFEDIEWIEEPIDVDTAEEPAVAEQNSEPEDQNTDXPKMGQQDPAGNQETYLDTKLEYTKDGSALVTETNRDGVMMDWETDIMXLAALALCKNYLDKAQDPEDECYNVLNIGFGMGIIDGFIQKTLETAKRQTGKPYKHYICEAHPDVLQKLRDDGWYDNPNIVILEGRWQDCLNDLLDKGTVFLDAIYYDTFSEHYQDMLDLYDLIIGLIKPAGVFSFFNGLGADNRFFYDVYKEIVVIDMSNYGLQCMYETVDVAKIMEKYGQVWDNVKQSYFSCKHYYHPVITFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R236</accession>
    <name>A0A1E5R236_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Hexose transporter HXT14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3882</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R236">
        <property type="interactions" value="1343"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R236"/>
    <dbReference type="InParanoid" id="A0A1E5R236"/>
    <dbReference type="OrthoDB" id="2241241at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF50">
        <property type="entry name" value="HEXOSE TRANSPORTER HXT14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="68"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="148"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="304"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="343"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="372"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="401"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="438"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="469"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="70"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="10">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="516" mass="56040" checksum="9E18DF4A2D7B614F" modified="2017-01-18" version="1">MTASEIETSVPSPSTPLPEETFKASSLSKDNLNSFSSIVPDSTSSYNINKEKPSNSGGIPTPSVKKSICFCILIAMCGFIFGWDVGTIGGITNMYSFKQTFGTIDTNGIKTLPNMLIGVIISIFNVGCAIGGLTLAKISDYRGRKMGMYVALFVYVIGVLVQISIPPGSSGKWYQFLVGRILTGLGVGATSVLGPMFIGETAPLRIRGAMVVMYQLVVTAGILMGGVVNYICKKAYSDTADNHGQWQIPIALGFAWAAIVGLGTFIAPESPVYLANKDKKIDNIDVGLNQKSNWKESFVGKPRYGLRLLIGCAVMIFQQTSGINYFFYYGTSLFDRVGLQDPYLTAIILGAVNFVATFGGLYLVEKLGRKKLLLVGSFGAFVSMMVYASVGSFAFQKHASGGIMIAFTCFFIIFFAIALGPVTFVVVSELYPPRVKTFSMAIATSLNWMSNFLIGLLTPTIXARIGYKFGYVFAAFMLASTFFVFFFVPETKNKNSDEIDKMYGKKMNKSSDTENI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R241</accession>
    <name>A0A1E5R241_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Vacuolar segregation protein PEP7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4112</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R241">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R241"/>
    <dbReference type="InParanoid" id="A0A1E5R241"/>
    <dbReference type="OrthoDB" id="166134at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098588">
        <property type="term" value="C:bounding membrane of organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.20">
        <property type="entry name" value="Rabenosyn, Rab binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021565">
        <property type="entry name" value="Rbsn_Rab-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036531">
        <property type="entry name" value="Rbsn_Rab-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11464">
        <property type="entry name" value="Rbsn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140125">
        <property type="entry name" value="Rabenosyn-5 Rab-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="7">
        <location>
            <begin position="148"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="7">
        <location>
            <begin position="288"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="84"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="553"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="112"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="586" mass="66821" checksum="20798A599F320520" modified="2017-01-18" version="1">MGRRLGEEAIAAKAPLVPISLDKGSELHAAAAXKKDVPTKVQNNDQNMHAKQVVECPVCPESFKNLRKLNKHLDHDHGFLDHEEESETLFKPNSVVDARKPQPXISKENQTDDQQVSRDSPITASDTSDSSRHVKQRFKLITSHYQQKHQQNTCSTCHSKISIQNSINCRKCGKIQCHKHCRNAIRLNSLAKYDPTNGEWCRCCPTCFGEKPGRNDVGTEVSNFESFKVLRKKHLEDSELRILQLDMRLVKLINGINRILEQHKYSFISGFQVSNDISKLEKTLVGWDETPSVCSACNKSITSITSKKHHCRLCGLYICNSNECSKELPLLLLCAVIESVNEDNFAKLPMDLRYTHNAVLHPDIMLRFCRTCTRLSFTGKKFKMDLKQPLPPLLQNFEKLFNMMIVLNSLIPQFSQMIDVATGGKTMTATERKKFLLFKKRIFGTFEMFEFTNKQILTLECESTAQKTIQKNMSLAATSFIQTQLVPLRSKLSVVLEKDLINEQMSKRDSTKNVIKTDRTTQDIKNINTNREELMVLQEQKFIVENMIVAAKNERKFDEIEALQTNIEDIEKSIAELQKALGSDGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2G9</accession>
    <name>A0A1E5R2G9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">26S proteasome regulatory subunit RPN1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3907</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the proteasome subunit S2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2G9">
        <property type="interactions" value="1581"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2G9"/>
    <dbReference type="InParanoid" id="A0A1E5R2G9"/>
    <dbReference type="OrthoDB" id="10252509at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016643">
        <property type="entry name" value="26S_Psome_Rpn1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002015">
        <property type="entry name" value="Proteasome/cyclosome_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041433">
        <property type="entry name" value="RPN1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040892">
        <property type="entry name" value="RPN1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01851">
        <property type="entry name" value="PC_rep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18051">
        <property type="entry name" value="RPN1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17781">
        <property type="entry name" value="RPN1_RPN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015965">
        <property type="entry name" value="26S_Psome_Rpn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 8" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RPN1 N-terminal" evidence="6">
        <location>
            <begin position="49"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="26S proteasome non-ATPase regulatory subunit RPN1 C-terminal" evidence="7">
        <location>
            <begin position="962"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="175"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="666"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="195"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="666"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="729"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="8">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR015965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ81092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1018" mass="112218" checksum="42AEFA2BDBDF5028" modified="2017-01-18" version="1">MSGPQDKDVDMDKTLAKPSNVVDQKKSKKEQLKDEEELSEEDMKLKEDLELLVSRLMEPDQSLYEPSLSQLKEYIRNSTSSMTAVPKPLKFLRPFYPKLTELYTLWTSTELKSLLADVLSVLSTTYSDNGKHDSLKYRCLSQEKNIEQWGHEYIRHLALEIGEVYGELILEEAELEENENDDETVKLENESSQSAEKPATKENTAAAAAXSEKENEDEKELAALLSEEFSKDDLLALSYEIVPYFLKHNGEADAVDLLSEVEHIHTLPQFVDADTFERVCHYMVSCVPLLPQQDATSFLQTAYSIYLSENXLSDALFLAVRLDDENLISQVFDAANNDPPMKKQLAYILSGQHSXFQKDEEVNAIIGNTKQSEHFQYLVKELNLLTPKVPEDIYKSHLETSGPSFGSGVDSAQQNLAASFVNGYLNMGYCNDKLVLNDDNWVYKTKGDGMTSTVASIGAINQWNLDGLQYLDKYLYVEEPEIKAGALLGTGLAACGVHDGDVEPALLLLQDYIESSNPKISTAAILGLGISFAGSKNDELLGLLLPLVEDVATPIQTSAVAALALGHAFVGTCNGDITSAIMDNLLERTDVELKTEWTRFLSLALGLLYLGMGYAADDVLETITAIDHPIMDSIEVLVMSCAFAGSGDVLLVQDLLHKLTPKLESTLEDGEEVEDEEDXLDEGPHGFENAPVPSAPAXEDQAEEGQSDNATAPKGTSXEMEVDETQASPEKETKKAENADESKTEDTEEDKAEKELGKADESIFSVFGIAMVAMGESIGKEMSLRHFGHLMHYGNEHVKKAVPLAMAMLSTSNPEIGIFETLSRYSHDEDLDVASNAIYSMGLCGAGTNNARLAQLLRNLASYYSNSQNMLFITRLAQGLVHLGKGTLTLDIYNDAQIMNKGTLASLLTVMVGLSSPTFMIDHHYLFYYLNAGIRPKYIIALNEDEEPIKVNVRVGQAVDTVGQAGKPKTITGWITHSTPVLLNHGERAELESNEYISLSSKIEGVVILKKNPDFKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2J5</accession>
    <name>A0A1E5R2J5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable electron transfer flavoprotein subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3855</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </cofactor>
        <text evidence="6">Binds 1 AMP per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="6">Binds 1 FAD per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ETF beta-subunit/FixA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2J5"/>
    <dbReference type="FunCoup" id="A0A1E5R2J5">
        <property type="interactions" value="478"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2J5"/>
    <dbReference type="InParanoid" id="A0A1E5R2J5"/>
    <dbReference type="OrthoDB" id="276685at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01714">
        <property type="entry name" value="ETF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012255">
        <property type="entry name" value="ETF_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033948">
        <property type="entry name" value="ETF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN BETA-SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294:SF8">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000090">
        <property type="entry name" value="Beta-ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="7">
        <location>
            <begin position="26"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ81121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="267" mass="29723" checksum="32870FD04619E7D9" modified="2017-01-18" version="1">MSKKLNILVPIKRVIDHQLAPRLSATNKTQITIPSYSINPFDDIAIEECIKLKEKHKDLIDKIKVVTISSDPSQTNKLKDILYKSLAKGCNEAKIIEVEGVAGKLLQPLTVANILKKEVLDNSNNLVILGKQAIDEDNNNVGQMLAGLLNWPQATNAAKVEILDESAGKIQVHREVDSGEEIVKATWPMVISTDLRLNVPRYATLPKIMKAKKQKIEISKIKLDAELSKINEAVKVLEYNEPEKRQPGIKVDSVDELLAKLKETKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2Q4</accession>
    <name>A0A1E5R2Q4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="16">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">AWRI3579_g3920</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">ATP-dependent RNA helicase required for ribosome biogenesis. Involved in the release of U14 snoRNA in pre-ribosomal complexes. Required for pre-rRNA cleavage at site A2.</text>
    </comment>
    <comment type="function">
        <text evidence="16">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with the U3 and U14 snoRNAs. Associates with pre-ribosomal complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="16">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DEAD box helicase family. DDX10/DBP4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="16"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2Q4">
        <property type="interactions" value="1108"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2Q4"/>
    <dbReference type="InParanoid" id="A0A1E5R2Q4"/>
    <dbReference type="OrthoDB" id="10259640at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17941">
        <property type="entry name" value="DEADc_DDX10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001632">
        <property type="entry name" value="RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15 21" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8 16" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="20">
        <location>
            <begin position="41"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="72"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="284"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="602"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="667"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="14">
        <location>
            <begin position="41"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="670"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="697"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="723"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OEJ81164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="776" mass="88498" checksum="18136280E025020C" modified="2017-01-18" version="1">MARKLKYNTKQRKDNRQKESDYINDLNTKIQEYDTKTSTSQFFEDLPISDKTVKGLKENAFLKMTDIQKESIPMCLKNFDVLGSARTGSGKTLAFLIPVLEKLYRERWTEFDGLGALIISPTRELAMQIYEVLVKIGKYHSFGAGLVIGGKDVKFEKERISKMNVLIGTPGRILQHMDQTIGFNTSNLQILVLDEADRCLDMGFSKTLDSIVDNLPASRQTLLYSATQSQSLQNLARLSLTNYKTCGESINNXVMNKKDGNNKNNGGTPEALKQSYITVPLPDKLDILYSFIKTHLKNKIIVFFSSSKQVHFVYETFRKLQPGISLMHLHGRQKQTARTDTLDKFSRAQHVVLFATDVVSRGIDFPAVDWVVQLDCPEDVDTYIHRAGRSARNGKAGENLLLLTPQEEPGMLARMKTKHIEPTKLNIKQSKKKSIKPQLQSLLFKDPELKYLAQKXFISYVKSIYIQKDKEVFKFEDLPSEEFAKSLGLPGAPKIKFKGMKSFEKAKELKNSSRQLQLLARTNENGDLVNEKSDKPRTKIDKMFQRKNQNVLSDHYMNITKQQAAGGEDKEEEEEDDFMTVKRTDHALKEDELPELIVPTSKRAQKKALSKKASLSTKGNPTRLKFDDDGTAHPIYELIGEEDFHKEGDVDTQKKSFLAKESELMANADITDKELAKEKKQEKKRKRLEQMRKEMEADMSDASEEYEDDAAQDYDIGTGNLSDDMESEDEEERSYKKRKYTNNSSESDMEDDNVIEVEEPQTLEDLESLTAKLIQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4U8</accession>
    <name>A0A1E5R4U8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyridoxamine 5'-phosphate oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3516</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4U8">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4U8"/>
    <dbReference type="InParanoid" id="A0A1E5R4U8"/>
    <dbReference type="OrthoDB" id="5300823at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052841">
        <property type="entry name" value="PMP_oxidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28040">
        <property type="entry name" value="PYRIDOXAMINE 5'-PHOSPHATE OXIDASE YLR456W HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28040:SF1">
        <property type="entry name" value="PYRIDOXAMINE 5'-PHOSPHATE OXIDASE YLR456W HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="PNPOx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyridoxamine 5'-phosphate oxidase N-terminal" evidence="1">
        <location>
            <begin position="6"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="201" mass="22690" checksum="5DBEEDB13842BA24" modified="2017-01-18" version="1">MSNEFPDHLVSLLKTSKYLHLATASKDLIPSCSIMHYLYVNQSKTYTKEGHDLIILATPEDSSKFQNMLENPNVALLIHDWVTAKKISLAKNSTSEVNLIDKESEQHQKLQQSGQXNRLLNLLQEMNQAELSQMSATLNGQVSIVDCAKSPEEYGYYKDLLLKDDPDAHAYVDNENERVAIIKVKVNSVKISDNENNTKTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4Z7</accession>
    <name>A0A1E5R4Z7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Eukaryotic translation initiation factor 2 subunit gamma</fullName>
            <ecNumber evidence="2">3.6.5.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3529</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4Z7"/>
    <dbReference type="FunCoup" id="A0A1E5R4Z7">
        <property type="interactions" value="1190"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4Z7"/>
    <dbReference type="InParanoid" id="A0A1E5R4Z7"/>
    <dbReference type="OrthoDB" id="1045173at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01888">
        <property type="entry name" value="eIF2_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03688">
        <property type="entry name" value="eIF2_gamma_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15490">
        <property type="entry name" value="eIF2_gamma_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000009">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000011">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000065">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050543">
        <property type="entry name" value="eIF2G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015256">
        <property type="entry name" value="eIF2g_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044127">
        <property type="entry name" value="eIF2g_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044128">
        <property type="entry name" value="eIF2g_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003077">
        <property type="entry name" value="PRK04000.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09173">
        <property type="entry name" value="eIF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 12" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="92"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ81986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="521" mass="57103" checksum="BE2BC90B337E14B7" modified="2017-01-18" version="1">MSDKLGNTVEEVVDDNEYQEEHDEYLDEQNGLQDGQTEKPKKSVAFSGLEDTEEDEEARKREFEEGGGLPEQPENPDFSQLTPLSAEIINRQATINIGTIGHVAHGKSTVVRAISGVQTVRFKDELERNITIKLGYANAKIYKCQDPSCPEPDCYRSFKSDKEIAPKCQRPGCEGRYKLIRHVSFVDCPGHDILMSTMLSGAAVMDAALLLIAGNEPCPQPQTSEHLAAIEIMKLKHVIILQNKVDLMKEESALDHEKSILKFIRGTIADGAPIVPISAQLKYNIDAVNEFIVKTIPVPPRNFLASPRLIVIRSFDVNKPGSEIQDLKGGVAGGSILNGVFKLGDEIEIRPGIVTKDSQGKLQCQPIYSNIVSLFAEHNDLKFAVPGGLIGVGTKVDPTLCRADRLVGQVVGAKGHLPSIYTDIEINYFLLRRLLGVKTDGSKQAKVKNLEPGEVLMVNIGSTATGARVVAVKADMARLQLTSPACTEVNEKIALSRRIEKHWRLIGWATIKKGTTLEPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4Z8</accession>
    <name>A0A1E5R4Z8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Regulatory protein LEU3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3530</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4Z8">
        <property type="interactions" value="487"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4Z8"/>
    <dbReference type="InParanoid" id="A0A1E5R4Z8"/>
    <dbReference type="OrthoDB" id="2341546at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001216">
        <property type="term" value="F:DNA-binding transcription activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.240.10:FF:000003">
        <property type="entry name" value="C6 transcription factor (Leu3)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051089">
        <property type="entry name" value="prtT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845:SF21">
        <property type="entry name" value="REGULATORY PROTEIN LEU3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="98"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="233"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="276"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="825"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="936"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1062"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="23"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="241"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="282"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="825"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="867"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="939"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="986"/>
            <end position="1006"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ81961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1126" mass="125686" checksum="BF2D28C6392A2712" modified="2017-01-18" version="1">MSGSNQTEPSLSPPSLESTGGSADQNIPASASFSKTLRNPNMFEHVKEKTGTIDTQQDNEHGPEKALKRKYVKKKFPVLDKLDETNNISISSKRKKLACVECRQQKSKCDAHEKSPAPCSKCAKKNIPCILKENFRRTYKRAKSEAIESKFRELTESLSLNISPEELLNKIAAGKDAIFDREAFTKDKVIQLRKSLNNQQFAEKLELIRKHSTNFNESTMRMMLEKNTMRHTPATDVPQETSTFSSPFPSSSSPSYSNVNTASQLPKNILSASSIDSAQPLNNNNNNNNNNNNNNNNDDNNNREFSTELEPNQLWNPQLTQCTTKTLGEVTLEPSEISELYLDFVYNYHIFLPIVNVLKGPEAIHKLSPCLFWVIMLIGLRRNERLQRVSKTLSPLVKSILAEITISPIIRYLPQETDQPLLNVSSVYSVQAFLLYSFWPSLTSSLSADTSWNTIGSACFQAIRVGLNSAQFSTEYKNANVDFINEQIKTWVSCNTASQIIASTFGFPSFVTFDHSVLKYGEIASHGNRDTVFTIPEPIKQMMQIAHFQHQVTETMNSNKLNPLGIVDDQEKLPILKVLEQQLNELEIRLQKSDLDHIRRFLLLVSKVYLFAYYFNSPKTDQNIFYMNTAKNNDHESAIDDSGNSFVVNFQTLCSHNISYTTKIGLVKVYNASIELLQHANAIWISNHSIIKYFPGVFVLNIWQAACIICKLVHSSLAPMIDVELGTKVYNIAVSLTYNASVMKFDLAYRSSRIMKSIWVMYKKMYEQWKSEDRSSGLLSSDKXTGQENNTADYNLDLTIQSRLSVSVFFDCLYVLKQQTGLAKLKREQMDKEAEARPREGSSGEEKESAREIIDTIPLDPAPINAPVSSNTSSATTSPEGLMASGIPATIDQRIKSANKGRNHVTSLKSILNKSPPKDGTLMSNSAESGFSXIARPQKTGSSVNSAPETTNVFSPTPSRSDSEPVSSRNATSLLNQSXLKKDLGNGNSIQGVTNXTTHNQHGQXLSTAQKXSNFPANNXPELENSASTAIQPKPSFEGLFAGLNXVTERSRKRXEPLXKTPYLPETLVDGGDQQDRVEAPQTSGANSGINAIFSTNKDLFENWDTDPVWEDVDVLLNEFAFNPTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R512</accession>
    <name>A0A1E5R512_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">CAAX prenyl protease</fullName>
            <ecNumber evidence="15">3.4.24.84</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g3540</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Proteolytically removes the C-terminal three residues of farnesylated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 15">
            <text>Hydrolyzes the peptide bond -P2-(S-farnesyl or geranylgeranyl)C-P1'-P2'-P3'-COOH where P1' and P2' are amino acids with aliphatic side chains and P3' is any C-terminal residue.</text>
            <dbReference type="EC" id="3.4.24.84"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14 15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14 15">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Endoplasmic reticulum membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12 15">Belongs to the peptidase M48A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.84" evidence="15"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R512"/>
    <dbReference type="FunCoup" id="A0A1E5R512">
        <property type="interactions" value="868"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R512"/>
    <dbReference type="InParanoid" id="A0A1E5R512"/>
    <dbReference type="OrthoDB" id="360839at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07343">
        <property type="entry name" value="M48A_Zmpste24p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2010.10:FF:000002">
        <property type="entry name" value="CAAX prenyl protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027057">
        <property type="entry name" value="CAXX_Prtase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032456">
        <property type="entry name" value="Peptidase_M48_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10120">
        <property type="entry name" value="CAAX PRENYL PROTEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16491">
        <property type="entry name" value="Peptidase_M48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 15" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 15 18" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 14 15" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="103"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="327"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="371"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 1 N-terminal" evidence="17">
        <location>
            <begin position="42"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M48" evidence="16">
        <location>
            <begin position="242"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="314"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="13">
        <location>
            <position position="413"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR627057-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR627057-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU366005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF16491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ81990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="474" mass="54484" checksum="156288E528EDECA3" modified="2017-01-18" version="1">MSLSVIDMVKGMFSTSTFPYKKVMIFFTLCDFAFETYIALRQYKVLQNKDKKLPHVLKGKIDEQKFKDSDNYGIAKAKFNFVSNTYDLFLTLYNLAANIQPKIWNYSILISSIVYSSIFQKSSSTFPQFSLLTQNILFFNFSVIFTNLLSLPVSYVQHFVLEEKFGFNKTTLKTWIMDTIKSNALIVLLGSPILYLLGWLLDKFSDNRNFVLYIWLFCLALQITMFLLSSTVIEPLFNKFTPLEEGELKTKIEDLAKECGFPLSDIFVIDGSKRSSHSNAYFSGLPFLKKRIVLFDTLIKDSSVDEVVAVLAHEIGHWKMNHTTKMLAISQIHFALFFKLFASTYKNFYLFKDLGFVVDPTQSQISHTGDVAVVQNFPFFVGLMLFGYLMKPTDSIHNFLFNLYSRHNEYEADEFAVKLGKGETLCDSLISLHVSNLSTVSVDPLFSKYHYTHPTLPERLGALENKITEQKKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R546</accession>
    <name>A0A1E5R546_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Nuclear GTP-binding protein NUG1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3674</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R546"/>
    <dbReference type="FunCoup" id="A0A1E5R546">
        <property type="interactions" value="1107"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R546"/>
    <dbReference type="InParanoid" id="A0A1E5R546"/>
    <dbReference type="OrthoDB" id="10266128at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04178">
        <property type="entry name" value="Nucleostemin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1580.10:FF:000006">
        <property type="entry name" value="Nuclear GTP-binding protein NUG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000844">
        <property type="entry name" value="Nuclear GTP-binding protein NUG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014813">
        <property type="entry name" value="Gnl3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050755">
        <property type="entry name" value="TRAFAC_YlqF/YawG_RiboMat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089">
        <property type="entry name" value="GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089:SF30">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN-LIKE 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08701">
        <property type="entry name" value="GN3L_Grn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CP-type G" evidence="9">
        <location>
            <begin position="176"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="476"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="96"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="482"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="10">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ82026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="527" mass="59333" checksum="E43E45746A69077C" modified="2017-01-18" version="1">MRLRKKQSKRTSTRLKEGIKKKAAAQHRKEKKHAKKDVTWKSRNKKDPGIPSSFPYKNKILEAIEQKKQQDLEEKERKRLEKIELRKQQAAMNNEEYVEDEDMMSGTEEDEGGLGALMASAQQAAEDYEKDDSTSKKQKLAKDLQDLEVEDIELDFEHDDQSQYEGGELEKSRKAYDKIFKTVVDNADVVLYVLDARDPEGTRSRKVEEAVLQTNGEKKLVLVLNKVDLVPPFVLEQWLTFLKSSFPTIPFKASTNAVNSTSFNKKLTTSNTSNGLLESLKKYANNRNFKRSIVVGVIGYPNVGKSSIINALTSSRRGNSTTVCPVGNQAGVTTSLREVKIDGKLKIMDSPGIVFPSELNTKNKVDKEAELALLSALPPKYIVDPYPAVLKLVKRVSRSDEMTESFKKLYELPPIPANDPNTFTKHFLIHVARKRGRIGKGGIPDITGAGLSVLTDWRDGKIMGWVLPKSSKQSSLQAIEEQQQQQQQQQQQQQQPVEQTSIVSEFSAEFDLDSLFASLDSAIEEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R591</accession>
    <name>A0A1E5R591_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="3 7">2.7.7.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3613</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a central role as a glucosyl donor in cellular metabolic pathways.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>alpha-D-glucose 1-phosphate + UTP + H(+) = UDP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the UDPGP type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9" evidence="3 7"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R591"/>
    <dbReference type="FunCoup" id="A0A1E5R591">
        <property type="interactions" value="920"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R591"/>
    <dbReference type="InParanoid" id="A0A1E5R591"/>
    <dbReference type="OrthoDB" id="932129at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006078">
        <property type="term" value="P:(1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-alpha-D-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00897">
        <property type="entry name" value="UGPase_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.10.10:FF:000001">
        <property type="entry name" value="UTP--glucose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000002">
        <property type="entry name" value="UTP--glucose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002618">
        <property type="entry name" value="UDPGP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016267">
        <property type="entry name" value="UDPGP_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43511">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01704">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000806">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 10" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000806-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000806-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ82077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="515" mass="58342" checksum="BB28111F5E7FFF45" modified="2017-01-18" version="1">MNSTKHHVKTHSTYQFESNTNNVAASQMRNALNKLIDSTLSNPKLSNETRAKFEKEMDSYFSLFTRYLNEKSNLSNKLQWDKIKSLTPKEIVSFQDLMSSKNSSSSKEDSTDLSSNLNKLAVLKLNGGLGTSMGCVGPKSVIEVRDGNSFLDLSVRQIEHLNRKYDSDVPLLLMNSFNTDKDTQHLIKKYSGNRIRIRSFNQSRFPRVFKDSLLPVPQDFDDSLDSWYPPGHGDLFESLYHSGELDALIEQGIEVLFVSNGDNLGATVDLNILQYMLQTDSKYIMELTDKTRADVKGGTLISYEDHIQLLEVAQVPKQHVDEFKNIRKFKYFNTNNLWINLKAIKHLIESDTAKMEIIPNEKQIKRNGQDYNVLQLETACGAAIRFFSNAHGVVVPRTRFLPVKTCSDLLLVKSDLFQLQHGELKLDPSRFGPNPLIKLGSHFKKINNFQERIPHIPKIVELDHLTITGNVFLGKNVTLKGTVIIVCSEGEKIDIPNGSELENVVITGNLQILEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5H4</accession>
    <name>A0A1E5R5H4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Eukaryotic translation initiation factor 4E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3826</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R5H4"/>
    <dbReference type="FunCoup" id="A0A1E5R5H4">
        <property type="interactions" value="1316"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5H4"/>
    <dbReference type="InParanoid" id="A0A1E5R5H4"/>
    <dbReference type="OrthoDB" id="590761at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019770">
        <property type="entry name" value="TIF_eIF_4E_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF8">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00813">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6 7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ82151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="222" mass="25665" checksum="67137047AE71B012" modified="2017-01-18" version="1">MSVEEVTQKTEQIDLSADQKQTVKHPLNTEWTLWYTKPQIDPSEDWSSLLRPIAKISSVEEFWGFYSCIPRASTLPPKSDYHLFRNDIRPEWEDPANSKGGKWSVFISDSKKPVLDELWLKLMLSAIGELLEEDDSVAQEVNGVVFNCKKKACKFGIWTKSLDNEETLKKIGLKFKNLVDTKLSTPVNEEQQQNSHPRKPEEIVFQFMKHDARRDEPAKITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7I4</accession>
    <name>A0A1E5R7I4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">COMPASS component SWD3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3333</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7I4"/>
    <dbReference type="FunCoup" id="A0A1E5R7I4">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7I4"/>
    <dbReference type="InParanoid" id="A0A1E5R7I4"/>
    <dbReference type="OrthoDB" id="674604at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="70"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="111"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="182"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="239"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ82854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="408" mass="45268" checksum="2205CB25058498FB" modified="2017-01-18" version="1">MLKTLESLKLPNNEDCCSTSLAISPNGKYVATVINTYHIIVYQLNQHNQRDALASGDSSVGYFQEFKKFTTDHSKGINSLTWSPDNQCIATCSDDCSIEIYHMSFGKLQTLMGHTAPVITIKYSPKGNLLFSGSMDESIKIWNLGSSGIVQPETSFQNQNEHDNTGENLYHTLLLTPPMKTMSAHSDPVVTLDIPLLDSSIIASGSYDGLIRIFDTKSGHCLKTLTYDKDWANDGVLPIINVKFSYNGKYLLVKSMDGVLKLWNFITGKVMRTFVESFTGTLDGELPDVDMADEQASSSNIERESKKHNTKDQMEDVANVNDDEISLTGRHCTGMEFLYTESGIPLIVDGTETGDLIVYDVNSKKILSKKKLSTSCVIDLQVYKQLVCTLTLDGKLELFHWSTSDDVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7J7</accession>
    <name>A0A1E5R7J7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 9">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="1 9">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 9">Tyrosyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3325</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="1 9"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7J7"/>
    <dbReference type="FunCoup" id="A0A1E5R7J7">
        <property type="interactions" value="685"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7J7"/>
    <dbReference type="InParanoid" id="A0A1E5R7J7"/>
    <dbReference type="OrthoDB" id="337870at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00805">
        <property type="entry name" value="TyrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.240.10:FF:000001">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024088">
        <property type="entry name" value="Tyr-tRNA-ligase_bac-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766:SF0">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766">
        <property type="entry name" value="TYROSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 9 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ82885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="458" mass="51699" checksum="3D9336BDA042E8B3" modified="2017-01-18" version="1">MLTTRRATTVFTRHLYSNQGIYHGLRARNLISQVSEPLAPFLHLVEKNPDKIKLYSGADPTASSLHLGNLVPMMVMLHFYLHGHDIVPLIGGATGKVGDPSGRTTERQQMEDQIRRANIANIQKQQVEFFKNGLQYYLSIYKNNSAPVVGKVSPMNNVEWWKDVKLLDFLANYGKHIRVQHMLSRDSVASRLSSKEGLGFNEFTYQILQAYDFLHLNNEHGVTVQIGGNDQWGNITAGIDLIQRCGKMQPRPIGYGITVPLLTTANGEKFGKSSGNAVFIDPTITSPYDMYQYFINVDDRDVERFLLLFTLLPLETISQIVEKHRLKPELRFGQKQLAKNVVDLIHGMRGADCEKISSVLFNNEKLIEMTATDIIKLFESGGKLIKKDKSQTLIEVVSSIENCSRSEVIRKLKQSSIYLGPNKFRPTKDVSDLSPYLIEGRVLILRVGKSKVHVIKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7P1</accession>
    <name>A0A1E5R7P1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Isopentenyl-diphosphate Delta-isomerase</fullName>
            <ecNumber evidence="4">5.3.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Isopentenyl pyrophosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Isopentenyl-diphosphate delta-isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g3356</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>isopentenyl diphosphate = dimethylallyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23284"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="5.3.3.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:23285"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the IPP isomerase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.2" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7P1">
        <property type="interactions" value="763"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7P1"/>
    <dbReference type="InParanoid" id="A0A1E5R7P1"/>
    <dbReference type="OrthoDB" id="510307at2759"/>
    <dbReference type="UniPathway" id="UPA00059">
        <property type="reaction ID" value="UER00104"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004452">
        <property type="term" value="F:isopentenyl-diphosphate delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050992">
        <property type="term" value="P:dimethylallyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009240">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006694">
        <property type="term" value="P:steroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02885">
        <property type="entry name" value="NUDIX_IPP_Isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000012">
        <property type="entry name" value="Isopentenyl-diphosphate Delta-isomerase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011876">
        <property type="entry name" value="IsopentenylPP_isomerase_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02150">
        <property type="entry name" value="IPP_isom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885:SF0">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 17" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0752">Steroid biosynthesis</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="16">
        <location>
            <begin position="96"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00083206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ82916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="282" mass="32730" checksum="C959E3D86087C72A" modified="2017-01-18" version="1">MNTKYHELVKSLNEQDILQEFPEIIPLKDRPNTHSSEDTAASDAASDKKISFFQGHDEEQIRLMNENCIVLDYNDNAIGAGTKKLCHLMDNINKGLLHRAFSVFIFNDKNELLLQQRASEKITFPDLWTNTCCSHPLCVDDEIGLENTLQSKVDGAKVAAVRKLDHELGIPQVETXARGQFHFLNRIHYMAPSNGPWGEHEIDYILFYKIQKGEDLTINPNLNEVRDYKYVTAQELQKMFKDENIKFTPWFKLICESYLFKWWNELSDLSKVEGDEKIHRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7R8</accession>
    <name>A0A1E5R7R8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent RNA helicase SUV3, mitochondrial</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g3235</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7R8">
        <property type="interactions" value="702"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7R8"/>
    <dbReference type="InParanoid" id="A0A1E5R7R8"/>
    <dbReference type="OrthoDB" id="3972200at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045025">
        <property type="term" value="C:mitochondrial degradosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000965">
        <property type="term" value="P:mitochondrial RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17913">
        <property type="entry name" value="DEXQc_Suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18805">
        <property type="entry name" value="SF2_C_suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000269">
        <property type="entry name" value="ATP-dependent RNA helicase SUPV3L1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001549">
        <property type="entry name" value="SUV3p ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055206">
        <property type="entry name" value="DEXQc_SUV3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022192">
        <property type="entry name" value="SUV3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044774">
        <property type="entry name" value="Suv3_DEXQc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE SUPV3L1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22527">
        <property type="entry name" value="DEXQc_Suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12513">
        <property type="entry name" value="SUV3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="216"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="376"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="13">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ82944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="745" mass="84416" checksum="FD8924DF94E2589E" modified="2017-01-18" version="1">MPFLRNTPARLLSGISTXNIRCPFSTTLIIXHLAKSYSTSGNLAXAKDTQLKLPENSNKAKKISELLQKVPTIAPSFADPSEFEAFQKLKSIIESSATTPTSSLNKPTFLDFLTLADLNKKLFYYLNHVNDVNDSQWKIVLKETDLSTPTLPLLDVASSRENFEVNTVSDDYKMIYILQKAFKMIQNKVQSEKAIKSNQQSLSNKFINNPVAWFPEARMMKRKIIMHVGPTNSGKTFNALQRLKNCERDGYYAGPLRLLAREIYERFKSENTRCNXLTGEEIIQDLDDIGNPANLTSGTVEMVPLNREFECVVLDEIQMMNDSQRGWAWSNIVLGCRAKELHLCGEQTAVPIIENLVKQTGDSLEIVEYKRLGKLKCEEATVKTKNFFQKSLLKGDCLIAFSKKTIMKLKLQIEKTTTFKVAVIYGSLPPETRLQQAKLFNEGHYDVLVASDAVGMGLNLSIKRIVFTSSEKFDGEKMIPLTISNIKQIGGRAGRYKSDSDGPSVGYVAATRADILESVKEGLQAPVIPLKTMIVWPPDHIIYKMIEDIEKLDNADLVTLLTSLSESLQKSTNKLFQLTDLSNRIESIQIIQPISGLSFQDRLVLSNAPCKFHRPXVKSAFYNFCLTIAKQQTCTITNFDFLPFRLIDLKYLNNDEVGSLEVYEDLHQTLQLYSWLSNRWPNYLVGVDTAKTLRDMCELIIFYKLENLEKNPYDRFIGKPQNRARFASERPATGIKPRGGYEKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7S0</accession>
    <name>A0A1E5R7S0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3439</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7S0"/>
    <dbReference type="FunCoup" id="A0A1E5R7S0">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7S0"/>
    <dbReference type="InParanoid" id="A0A1E5R7S0"/>
    <dbReference type="OrthoDB" id="1028014at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050869">
        <property type="entry name" value="H3K4_H4K5_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197:SF294">
        <property type="entry name" value="POTENTIAL PROTEIN LYSINE METHYLTRANSFERASE SET6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="1">
        <location>
            <begin position="11"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="386" mass="45304" checksum="EF067F525C5DA021" modified="2017-01-18" version="1">MTMLDDSEELLSLELEQMRPSQYYTIKQTSWGGRAVFASVDIPRGTAVLRCKEPMAYSINLDFKKECCHYCFNYFADEKSLKYKIIECDLWFCSKECLNSYEEKYKETGLFETFKKLKSLFSTIQKKNSQKLYNCFFDDNTSVPITKLAIDEMWNKINNKWIPSVDKLGHNKNKKLKLLNLECFTEQEYTECRYVFQCLTNLVKICSKTSEVQAKVRKLFVELQSNELETITSSPHELLMHLKIFQICYIILNHSKLGQKAKLSTKLFRHIMGSESANSFGIWEMDENLESEDREFLGTSLYPEASYFNHSCFPNISRTREGSSLIFTTNMEVKENEQLCIQYGGIVACKLPFEARQRQLYEKWYFKCLCVSCEQEKAKKRLVQKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R834</accession>
    <name>A0A1E5R834_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein TRE1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3449</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R834">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R834"/>
    <dbReference type="InParanoid" id="A0A1E5R834"/>
    <dbReference type="OrthoDB" id="5841748at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.40">
        <property type="entry name" value="Transferrin receptor-like, dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039373">
        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007365">
        <property type="entry name" value="TFR-like_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036757">
        <property type="entry name" value="TFR-like_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF72">
        <property type="entry name" value="ZINC METALLOPROTEASE TRE2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04253">
        <property type="entry name" value="TFR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47672">
        <property type="entry name" value="Transferrin receptor-like dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin receptor-like dimerisation" evidence="4">
        <location>
            <begin position="672"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="648"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ83067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="805" mass="92663" checksum="9984D4E2CEC98786" modified="2017-01-18" version="1">MPTRSLLNDSPQDESFESAEMPLEPPTYDDIEIGHPEEEMLLEDSXSEQVHWYSQNIKENSLVQVLQNIKDYLYDFVVIPFRLKVVRPLMEYYYFFCISYEYYLAKIGNPLIMKRFVYVFLMSIFAYGIFRATMDETARAVKGTFSDHDELLHYAKRCVDLAKFEQDLEYLSSMHHNSGTKGDLLFVNYIEESLNKNGIKLSLKSEYDTYLNFPTXKSTLSYYVLDENNEGQERQEIXLSNENFNPLSINGDLKGKXLLYNNYGTHTDYDALDEKSINYEDAVIVLKYSELVSEQILLAQEKKVAGIIFISRQDADDGPGKETTNQDSSSTEIGEKLLQESKYDDLVQMIGVGITQYGIGDPLSPGWSSNLPQKITLEHSNLVPKIPTLPVSINQIRPLLRTLLVDKQAVHVDMHVELDADERHPSWDLVAKIEGKEQNDKAIIIAAARDSVSFGASYPNFGTATLLALAQLFQQIKYKYNWKPLRNIYFMSYDSSMYNYEGSTEILEDKIMSIRHEVYAFMDITQLGIDSNNLFVQGHPIFHDFFNKLSDKFDFQIKTKNVQQYGGWTPYMAKGFPVVVLSSKSNLENSLPKFSKGDDWENMKKTLYKEYKDGNGWDRTSRLILYTLEVALGLADDPIIPFNFGTFTNSVYASYQDLEEEVKNLDEEGKQIDLSPIKRALDTWTFVVKEWEHILFIWNQIVNEDGNHEEPSLIAIHRWAWNRKLAATPLKQVDSKGISNERRSYKNIIFGPTLWSSSSYEWWSFPGVRDALRSKNWDSAQESVNAVGDILQKTAIMFKEEDAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R861</accession>
    <name>A0A1E5R861_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Phosphatidylinositol N-acetylglucosaminyltransferase GPI2 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3293</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R861"/>
    <dbReference type="FunCoup" id="A0A1E5R861">
        <property type="interactions" value="542"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R861"/>
    <dbReference type="InParanoid" id="A0A1E5R861"/>
    <dbReference type="OrthoDB" id="196709at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000506">
        <property type="term" value="C:glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009450">
        <property type="entry name" value="Plno_GlcNAc_GPI2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12982">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN, CLASS C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12982:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06432">
        <property type="entry name" value="GPI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ83099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="286" mass="33857" checksum="24F21575D43724AB" modified="2017-01-18" version="1">MKQWQRLLWVKQDYPDNYTDPDFLKQLRYHNLNEIKPLSTVAYKTIFKKNYYKCFKFFENLINISIMYIVYEYFLHTKKEFLFTKTVLLIVSLPSVVIISKMLESVKSTIIILFTILTLSPVIKSLSKTTSSDSIYNISFWLSVLNIVLLTTTSSQSKKKKSTNIIKINLILSNLIILASRLSSTVQVFMFSILAIQLNILIPFYFKRNVCYTLFEHLLVYYWIYHTQRKLQISVPIAIVVATLFMVAENLLILLSTYYLTYWQQTYYTRDNLLLDRWDVSKPILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA05</accession>
    <name>A0A1E5RA05_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Nucleoporin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3007</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the non-repetitive/WGA-negative nucleoporin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA05">
        <property type="interactions" value="1201"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA05"/>
    <dbReference type="InParanoid" id="A0A1E5RA05"/>
    <dbReference type="OrthoDB" id="338970at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044611">
        <property type="term" value="C:nuclear pore inner ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036228">
        <property type="term" value="P:protein localization to nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000972">
        <property type="term" value="P:transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.440:FF:000001">
        <property type="entry name" value="Nuclear pore complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.440">
        <property type="entry name" value="Nucleoporin, helical domain, central subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.450">
        <property type="entry name" value="Nucleoporin, helical domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007187">
        <property type="entry name" value="Nucleoporin_Nup133/Nup155_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014908">
        <property type="entry name" value="Nucleoporin_Nup133/Nup155_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004870">
        <property type="entry name" value="Nucleoporin_Nup155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042533">
        <property type="entry name" value="Nucleoporin_Nup155_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042537">
        <property type="entry name" value="Nucleoporin_Nup155_C_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10350">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10350:SF6">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03177">
        <property type="entry name" value="Nucleoporin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08801">
        <property type="entry name" value="Nucleoporin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup133/Nup155-like N-terminal" evidence="7">
        <location>
            <begin position="199"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin Nup133/Nup155-like C-terminal" evidence="6">
        <location>
            <begin position="810"/>
            <end position="1581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="27"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="31"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="84"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="8">
        <location>
            <position position="849"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ83732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1588" mass="180337" checksum="5228278B03550693" modified="2017-01-18" version="1">MFVLERSFLTLVQIEQILQDCTKTTAKKDLNPQNNSRAAMSLYSTPLKTTSGVRRNTEDNNIPLSRKNFNSVANPFVASKTGPTNTNITTNNKITKPSAAGTHNGTAASRLTSTKFFNPQAYSMMRAPFGNMFGMVASGRSENIEIKNMAEQSPLELASQYVESLQELDNSTPPLDMNSYYSNGVEYNYSKEIGGLGPLTPFERVKVLNIPDELLQKFKESSLKKSTTTQCGIFPEIDRCWFTIDNKLVLWSVKQPNVFQTLDDDFEHDIVKVALVKPQTNTFIHTIEHLLIVATHKDMHVLAISYDENSEDLKVFNTGLSVPLNGLFVNEIVSNSKTGQIFFTAKNDFNVWEFQYSNNDDWFNSKCSKICLTQSFLSHLVPTGFVSKISSFFGTDHNLEHITKLVIDESRGILYTLSSKNVIKGYGLNKNRKGIDYTIVINSHDISTKLKMNNNWRSPLFSNEYFKIVSIVPVSRKENNSLCLVAITVAGCRIYFTGNQGYNSSSLYSNTINNGNVRFTSLTIESVKFPPVTSEVVAEKMNKEISERGKLMMSNNVYNSFDLWNYKLQRNSSTLLSTSTNATIISPGIFFAPVVKKDSKKNENVSHKLYCSVPDYGILKNYSRYXENATFLDTTGDIKCVTPVTQLFNATKTPYGYANEFATQYXREPLQIAVLTSTSLEIYKYRTPDEVFESFLNSGSVEPFVMNNGQAEACSTALFFACKLNKSEQSRVSALNFFLLGIPNVIDLRPTYNTRMTATSFPSAISNGSTLAASSIGNSSATGNLSGGGSTSTIHETLHNELSLDNVLLSPRFYGIALLVSRLCRDIWNKPIFELMGNIKIVDGKILELSTDSTDDSNLNSGNTAVDAKNXGKQLLSKISVSKDELEYYISSIIVLEEFLNKYHDQIICTKATSSTTVGGSSVGSGSLLTKQEEIAYQAENIGINSLIMLIKSIKEALSFLNVLYEESEINGFEGQYLAFKDIIKYLKIDVQLKLSELKFKDFFTGSNSLEVKTLIREVSSSIINRNLLRGRSIDYITNALQERCGSFCSSNDVLGYRAVEHMKKAKEVGLRDVEVLNYHLKNATIYFTKICDDISLDKIKEACWIMLDLNYYPKAIEFLLKMCQSVDKGNLALQYVNDGKLEHDERKKYYDHRMVIYDLIFQILIRLDNEKVITRNGGNSASVIXKTPLKGSQYKEVQAETFGTIPSLEVLEKLRKETYHIVFQNDDKLFHYSLYDWLFEQHQQDKLMEIDTPFILPYLKEHSVDSLEACNMLWIYQSKRSRFYESALILLSLATSNFDIELSKRIEYLSRANGFSKSMCSPNERSKMIKLSSDIKEIFEVAEIQDEILKMVENDSRLMGEDDPKVAHSGKTTVTTQSTMISQLNNKILSVSDLFNDYAEPLEYYEICLLIFQISDFRVHEEIIKKWKQLFVSIQQTQQNDSNPNDLKYLLSNLMVSLGKKLYTNDFVFPVGELIQLIVEFFYNGNTNPNNDNQQISYKTGEIIDMFINCGVPYDKLYYILKNLIETNNFVITIYKKEMIYLIQNWYKEDRNLRDIIKYEQIRSLPEVYELXKDPINEYTEKTGDRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA36</accession>
    <name>A0A1E5RA36_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">U3 small nucleolar RNA-associated protein 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3135</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the ribosomal small subunit (SSU) processome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
            <location evidence="1 9">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the HEATR1/UTP10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA36">
        <property type="interactions" value="1172"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA36"/>
    <dbReference type="InParanoid" id="A0A1E5RA36"/>
    <dbReference type="OrthoDB" id="31183at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034455">
        <property type="term" value="C:t-UTP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012954">
        <property type="entry name" value="BP28_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056473">
        <property type="entry name" value="HEAT_Utp10/HEAT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022125">
        <property type="entry name" value="U3snoRNP10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040191">
        <property type="entry name" value="UTP10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13457">
        <property type="entry name" value="BAP28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13457:SF1">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08146">
        <property type="entry name" value="BP28CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23243">
        <property type="entry name" value="HEAT_HEATR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12397">
        <property type="entry name" value="U3snoRNP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01036">
        <property type="entry name" value="BP28CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 9" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 9" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="BP28 C-terminal" evidence="10">
        <location>
            <begin position="1530"/>
            <end position="1677"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="8">
        <location>
            <begin position="1769"/>
            <end position="1807"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ83754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1809" mass="205130" checksum="F070A76D3839AC9E" modified="2017-01-18" version="1">MSSLRDQLSQIAQQNQGVALDRKKRSKIHSASLIYNPKVASTQDYEYLFETATSALEELISIDPKFQVFTKSLFNESSINLDRNAITDEELKDLNSTINLFLMLINSRWNLTPTLHAAEWLVRRFQIHIHNADYLLFSSLQYYQLPVFKRILNIIKLPPLWSPLSNFVKSESKNPSNLTIIKLFQDFEFLAEYEKFLHQCIKKNFINSKLLCFTTCSLVNLLTHTTSKTENLFVSKIIEIVAKLLMAKNNHSHDCQMSAHTILVVLSKKNLDSKIIMAALETMLSNLHPMAHKSAFIAMASLCQSMDGTISVEQFPMKLYNLIASKFNNDIQNLIVVHGANFDKFLTSFIRSIIRYDQDKFPKVVQLLDTTILQTFEXKIIVNDLIHLSEIIEDKTKLVPXFEKLIAQNDEPLILSCLESLGLSGEIFEIRLTTSLFVNNINSGDTEKDIERIVRDVESSKIVGGMTDILPFKDWMSKNAQFCFTSNQSLLSANDDVFNKLLSLFLEALGKKYSIGLFLNSFLTTIEARITFLLRVTVSPASPVALKLVGLSHLSKMFNSIDKDLNLFTLVPVLVTGLFDVSKNVRLSIKKVLQQILKKTDSKNDNFLLIKSIYGDIDLSETMMSPSDSYVWLHTFLENYNVDNYDLSRSVIHKKSEKIDLLFWCYQIISLPLVFSKSCLLEIVTSCKGYNSNYSQLLQPVLETYLEQREHYKXSCLNNKFDFKKFESNVVNIISMKEKNQFCIDFVIKSLKSESEQLTEVVCKKLTSIFSSLKPAFQLEIVESIIESSASTLTTENGSENSISELSFDPLGLLQNLNIPSSIFVSILEKNRINADEDNDQMIKRRRRRRSSTAAKSALQKEEISHLAELHLSKITTILETLDSNKNIEGSGELLTCLFNHLSDLEVLEQDGGYSVLYAQETLSSCMLNVIESLKKTPQKPLHIRSDIVVSAIRSSPSPQVQNKLLLVIAALATLSPEMILHSVMPIFTFMGAHTIRQDDEYSTHVVENTIKAVVPALLSSSGSKKNELDFLLTTFAAAFPHVPKHRRVKLFTTLINTLNPELAVSPFLFLIGEQYSSSVQKFKISESKEFIAFCRSFLNKLAAVEQLKGILGVFKLIKKLPTSFVYFKSPEGMREEMLKETLFSNSIINLDFFGLNKLKKNLLQFINKLVVDDSNDYYSDGSLKLKILTLLTDEEADSNXVETAFSSLNKEILEGIEEVSFQVSKDKDFAHKDTASEITTDASGASQENGTDLKDNLLEIKQNLFELLKNILFLLPIDSYIQSVSSLLTAKYENSVRQHITLAIGERFKFESLETCPAAMEIMKIIFENSKNSESTTELVETSLATINDLISKFKDKIDSSMLTEALTISTXNVQSEHTSISIACLALMNNTLQIQGVKCISFYPKIIPAALKLMKKHENGSDPLRSQLQIAXILLFAVAIRKLPMFVVSDLQKMMVTIFFSDEVSEEIKLSVLKLVVQNIDLKEVLKTLHKIWQSDVFEKSNAALRTSSVAISLYLSILEATVDKIDKKAATAQSPIFFKLLLSLFEYRSYSAFDNNTLGRIEATVHKIANIYVLKLNDKVFRPLFALXVRWAFDGEGVKHKEISREDRLCAFFKFFNKLQDHLKSIITSYFTYLLDNTNKLLLEFSEGKKSNTNLRRLVLIAVGSSFKYDRDEYWRSTARFEVIGETLIGQLRNIEPTIGKYLVKAIGSLASCNKGSEEHNKAMSKLLISHMKSNCKSNEKLWAVRSVKLIYTKVGETWLTLLPQLVPIVAELLEDDDEQVEYEVRTGLVKVFENVLGEPFDRYLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA37</accession>
    <name>A0A1E5RA37_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flo11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2994</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA37"/>
    <dbReference type="InParanoid" id="A0A1E5RA37"/>
    <dbReference type="OrthoDB" id="4070545at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018789">
        <property type="entry name" value="Flo11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10182">
        <property type="entry name" value="Flo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01213">
        <property type="entry name" value="Flo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51824">
        <property type="entry name" value="FLO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184667" description="Flo11 domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="1515"/>
        </location>
    </feature>
    <feature type="domain" description="Flo11" evidence="3">
        <location>
            <begin position="17"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="682"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="724"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="774"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="824"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="986"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1426"/>
            <end position="1458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ83747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1515" mass="151494" checksum="3914C80CAECD79FD" modified="2017-01-18" version="1">MTQMLQYFWLALLLATQTIASKTCTPFKQCQQNNMQYFQNVNSVPYYFDLTQVEYLSGTSYQITYHIWSDANISLSSLNELKYLGSGVSSSNTMIYSLNTGAGLDMDFDPTDFTMSVTVDTSSANYGSELTGLPNSFGFQFDYCKGMNTYSSPKNSVFYTGNDYTCQCDWHYGATSFDYYTSCNGDNNCNSQAAFSNWLWPKECADITDQKQQMSSLSAGNAQSYCTYTGTTNTDWVTQIKNYYTPSYCSSYSTTTPKPSSATSTKTTTSVSSTSPSRSSSSAISSSSIFVSSQSFVQSTTQSSTVSSASSTQSSTVSSASSTQSSTVSSAPSSSAPSSSAPSSSAPSSSASSTQSSTVSSASSTTQSSTGSSAPSSSAPSSSAPSSSAPSSSAPSSSASSTQSSTVSSASSTQSSTVSSASSTQSSTVSSVPSSSAPSSSAPSSSAPSSSASSTQSSTVSSAPSSSAPSSSAPSSSASSTQSSTVSSAPSSSAPSSSAPSSSAPSSSAPSTQSSTVSSAPSSSAPSSSASSNSTISVSTQSLIQSSATFSSAQNFSTSFSSLSSTLPISSSSANFNYSTSSSSASTSVSPTLSSSSSAISFTASSSSSVYSSVSSYSVLTNSSFLTYPSSSGPSTTISLSSLSSFSSPVSLSSSNATTTLSSTLSSYSSVVSSSAFSVTSTVSPSTFSSYNNTVPSTSPTSTTTLSSTLSSYSSVVSSSASSVTSTVSPSTFSSYNNTVPSTSPTSSSVVPSASSSSVLSSYSSVVSSSASSVTSTVSPSTFSSYNNTVPSTSPTSSSVVPSASSSSVLSSYSSVVSSSASSVTSTVSPSTFSSYNNTVPSTSPTSSSVVPSASSTVSISTDSFVQSTESSSTTVLSSSAFLSSSSGIGSTSASSSSASGFSTPVNSSSAVISTTSSGIPPSSSTVLPSTSQSSSFLTSSSYVSSTSLKSFSTSSSFTFASRTSSVYPSYGNSTYVTSTSTLTSTASVSSDSSFSTKTLSSSSSASTSLPAISSTKTSTASGSLSSSASSSSCVVTSTVTSPQTVTSGSSTYTTYTTYVTFSTDSTCVLSSTSASGSLSSSAPGSSCVVTSTVTSPQTVTSGSSTYTTYTTYVTSSTDSTCVLSSTSASGSLSSSAPSSSCVVTSTVTSPQTVTSGSSTYTTYTTYVTSSTDSTCVLSSTSASGSLSSSAPXSSCVVTSTVTSPQTVTSGSSTYTTYTTYVTSSTDSTCVLSSTSASGSLSTSALTTTSVVTSTVTAPCPVTSGGSTYTTYTTYVVSSTITTCPLSSTTKMESSNTKTAYSTEPLSSRSTQTIVVASTVSSPYKVVSSGSTYTYTSVHTVLSTITTCPEGSSTTTLVDDFSKXSSAITSSEFAHIVTSTAGLSGETSTYVPTTSLTKVASSTSLVDDISEASSASSAFPVLPVTSTETRNTATSGSTKNGVSSVATDTTLSTSNTGKATSTTEASAYAYSTSTFVTGTISSASAQEAGAPKRAGSTMLNLLVSLTFTLFYMFFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAK8</accession>
    <name>A0A1E5RAK8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PHO85 cyclin-7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2741</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAK8"/>
    <dbReference type="InParanoid" id="A0A1E5RAK8"/>
    <dbReference type="OrthoDB" id="1060854at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20558">
        <property type="entry name" value="CYCLIN_ScPCL7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF94">
        <property type="entry name" value="PHO85 CYCLIN-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="301"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="597" mass="66451" checksum="9D7DF359691310CF" modified="2017-01-18" version="1">MSFTSNHSSHHNHASAEVPIAQPLPHYPNGHTTHVPQQSETTISAKSTPTAYTTPLSRTSSVAVPQTNNKSCRNTPLIETTNERSISVVNNEAVSTSSSSSSSSCSESYSLRSTVSGSLNVXVGGEHTEPLEQQEKTCAANDPNVPSPSXYNFNGTPSSYGSNASFNYHKLQKQKSGSKLYRVNSPMADIVKKNSKIRVGSHSSLIINHTNDPTDSNDGDHAGDDCAIAEDSDTEGMDEHKNLHDNGRPMVQNKKPELGARKESISDRFKRSLLRPYNDKEVLYTPSPVMMQEPHPGRGSGTSTPLNSGAVQNHYERPARRISQLTLSFQEQTPSPPPPPHSSSSLSQRSFSFGDHNITKLYGVPLLLNETQSSEYINNYLSKNKTKFQIYKRPVVPNNNDEPAGKESHESMEIATFPTDTLLSMLACLLNKIIQSNDHMMSAMEQERRSGENGHTKMNGISQNVNVLQFKGKTVPKITLEDYFHRIYKFCPTSNDVFVSLLVYFDRITKKFEKIGEKFIMDSYNIHRLIIAAITVSTKFFSDFYYTNSRYAKVGGLSLPELNNLELQILILCNFELIINVEDLQKYGDLLLKFCSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAV5</accession>
    <name>A0A1E5RAV5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Putative mitochondrial carrier</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2725</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAV5"/>
    <dbReference type="FunCoup" id="A0A1E5RAV5">
        <property type="interactions" value="343"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAV5"/>
    <dbReference type="InParanoid" id="A0A1E5RAV5"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="8"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="126"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="234"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ84034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="329" mass="36583" checksum="B4CCEE3D155C24AF" modified="2017-01-18" version="1">MKSKKEGTPMEVSLIAGGVAGITSRTVVSPFERVKILLQIQANNKSDVLSGKPTEGKKSIFQLINNMFKNEGVKGLFRGNGMNCIRVFPYLSIQYGTFETYKKYILQNKNLIKNSDGNTNRAEGQLPNVHRIIGGCLAGGTSVLFTYPMDLVKTRLSIQTAMAGKPRNGHSKPPGMVKLLVDTYKHEGGLPALYRGMYATCLGVVPYVALNFQIYEALKFLYINHMGNNKQESFNDLHKLCFGAISGGLGQIIIYPFDLLRRRFQILTMKQAGQDIGFHYTGVADALVQIGRKEGFKGYYKGLAANLFKVVPSTAVQWWTYEFVVNLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB64</accession>
    <name>A0A1E5RB64_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">General amino-acid permease GAP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3096</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB64">
        <property type="interactions" value="225"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB64"/>
    <dbReference type="InParanoid" id="A0A1E5RB64"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000017">
        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004762">
        <property type="entry name" value="Amino_acid_permease_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00913">
        <property type="entry name" value="2A0310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF1">
        <property type="entry name" value="GENERAL AMINO-ACID PERMEASE GAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="195"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="218"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="411"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="435"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="482"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="518"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="10">
        <location>
            <begin position="80"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ84140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="594" mass="64937" checksum="2ECCFCE953403299" modified="2017-01-18" version="1">MSDLEKNKGISYEDSSTASKLENVNSGTYAFNSSGSKWTDFKDSFKRVEMEELDPNLTAAEKIAIATARAPLQRHLKSRHLQMIAIGGAIGTGLFVGSGSALRTAGPAGLLIGYAISSSFIFAMICAVGELAVEFPVSGGFTTYATRFVDESFGFAANFNYMLQWLVVLPLEIVAASITVNYWQTPTRYRDGFVALFWLVIVIINLFGVKGYGEAEFVFSIIKVVTIIGFIICGICLVCGARGHYLGGTYWHHPYGAFVGSTDGARFKGTMSVFVSAAFAFAGSELVGLAAAETENPRKILPRAAKQVFWRITIFYIVSLTLIGLLVSHKDPRLVGTSSVDAAASPFVIALVSHGIKGLPSVVNVVICIAVLSVGNSAVYACSRTLSALSEQGFLPEKLQFGYIDRNGRPLVGIIFTSTFGLLCFIAACKKEGDVFNWLMALSGLSSLFTWGGICLCHIRFRRALAAQGRSTDELSFKSPCGVIGSWYGLIIIILVFIAEFWVAAWPTGFKEMTGGEIAEAFFETYLSFPIVLAMYLGKKIYSRQWRIFIPASEIDIDTGRRATDVEAMKAEVAEEKAYIASRQWWYXLYSFWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBA8</accession>
    <name>A0A1E5RBA8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2764</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TfdA dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBA8"/>
    <dbReference type="InParanoid" id="A0A1E5RBA8"/>
    <dbReference type="OrthoDB" id="93019at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051178">
        <property type="entry name" value="TfdA_dioxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43779:SF2">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT XANTHINE DIOXYGENASE XAN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43779">
        <property type="entry name" value="DIOXYGENASE RV0097-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="7">
        <location>
            <begin position="23"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ83853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="391" mass="43699" checksum="00B7829D54C04120" modified="2017-01-18" version="1">MPIKVTPIEGAPLGAKIELPEGCTDPSKLSTKDFEDLYKAVLTHLVVVIPGMADLSPESQWKLTTLFDKTCDQTGKSYGHGKEFRHAQSVLRKDGTSIPNQPQVQVLGQGKFPEGHYGMGELDLTHPTHFTFHKDPLAKEKSFNVEDDEIPLTRFYRWHLDSALYKLSPPVATTLLGIHVPPHGRMQKIVYEPEYGYDEPDSLELTQGATCFVSSAXAFDQLSEEEQKIALGTTIVYAPHPYIFISPAGATSDGLTMANEGKEMPLDELPEWEEAHVKKFPVVWTNPVTKKQHLQIHGCCVHKLILPDGTELQLEDARKKLHSWMRPAIASKNVYAHSWDAGDLCIFFNRGVWHSVTGQFDEGERRLMHQCNIASGEDPVLVLPEETEVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBD1</accession>
    <name>A0A1E5RBD1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Retrograde regulation protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3050</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBD1"/>
    <dbReference type="InParanoid" id="A0A1E5RBD1"/>
    <dbReference type="OrthoDB" id="690068at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="2">
        <location>
            <begin position="353"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="454"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="515"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="458"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="546"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="3">
        <location>
            <position position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="584" mass="65836" checksum="3E9967AF0789360D" modified="2017-01-18" version="1">MDFKLFEDSPTHTFGSISADSPHNQKNDEPTGSELQIPIDDIFRSSKNEQQDKDSSKPTTLGSSRNLVSNAAMDFQHLLEDDFFSRSLSASTNASMSPRLSSETNPMAXNNTSAMRSVQYQQRMKQNTPISNNNSYSGNDLNFDSFLKNKKINXYNAADFTQNNSPQQLEHTPQHATSNLPSTNTNFNIDNDFLETLAIFDNQNNVDSKKMNETAVXPLKTLPQTHPEQIDTFWYKDRFENLAQPATNVNSNGQTMFSPQYLNSVAKNDLDTASQISSSIYSSNSFATPSIISAGAGPLKNSSFGTGFGRPRRMSIGEESGYSGTSSRLLDSKSISGKSVKSRASRDDDRLHRKRSCHNEVERRRRDLIKYKINELNDLVPPTLFNRYMKNATTKPKKRIVLCGSIDYLKVLMNIIAMQKXNKKLLLTKIKEYEHLLDYAPDLKSNAPVVSGFENRLPDNNTTHSVTKTTSSIVKARTPTTSTPEYQQLNTYAPESLDFIKSEKDLNKLLMLSEKQEQRQQQQQQQKQERSIGSXPHVEAPKEISPPQSTQQQNAEQHALTENDLDQLLINDEQLNEFLRMTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBE3</accession>
    <name>A0A1E5RBE3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nucleolar pre-ribosomal-associated protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2477</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBE3">
        <property type="interactions" value="356"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBE3"/>
    <dbReference type="InParanoid" id="A0A1E5RBE3"/>
    <dbReference type="OrthoDB" id="72892at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059018">
        <property type="entry name" value="HEAT_URB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039844">
        <property type="entry name" value="URB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032436">
        <property type="entry name" value="URB1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021714">
        <property type="entry name" value="URB1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13500:SF0">
        <property type="entry name" value="NUCLEOLAR PRE-RIBOSOMAL-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13500">
        <property type="entry name" value="NUCLEOLAR PRERIBOSOMAL-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26140">
        <property type="entry name" value="HEAT_URB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16201">
        <property type="entry name" value="NopRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11707">
        <property type="entry name" value="Npa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="URB1 N-terminal" evidence="1">
        <location>
            <begin position="66"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="URB1 central HEAT repeat" evidence="3">
        <location>
            <begin position="586"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="domain" description="URB1 C-terminal" evidence="2">
        <location>
            <begin position="1427"/>
            <end position="1622"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="4">
        <location>
            <position position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ84217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1699" mass="194842" checksum="97FF306AD7BB2AB4" modified="2017-01-18" version="1">MSEXLDVPFENTHKKVWKQKVFEGNGVIKNDDANDAMYEXLQKVLTDFESNRDFRPLMQFLSKHQQQLVQSWSLYAQINNHKKVTLSLNRMTAILSKINQNAQDCPEQLYEICSEMVRSIVANYTKTLYRAINNMRGSLTNPTLRLMTEIVTLQNGKHIDEFISHFDFSLPSLPKLLIPSKAELADVELTKTKNQHLTLRYNFIKFYSALISHSPAILRKDILXDNSKLMSNWFKYISKIDSDEVINGAIDLLTDHIVKEPSFKKSTKCKIVNEYILMNLHKLYYSGNAKIASKVNAFFILYSTDPVYGVAFENHKSWTADIDESSRQNNGAQITVHNKSFKLLNKILYTMLTQFKPWEDDTQCSTVIKVLKHIPEFVSPYSHYLMAVHGSHEPKMTSYWFGQTLLLXRMIKLPIPQSVYELDFEYLPSMTVVLDNIMPLPITKAVLKKCLSNQVKLVQQMGCQLTVFILQKLADILXLYTVQGWNKEKPALLNSVFAKLPELSDFTSLLNDTYAKSSDNKLLILTITKILNLYSQHFPAATSITLPQNNIYSDLMAKSQQSEQPLSGIDLAILDEYLEFQQLNENYKWWNKDKSGHSLFTALLKICSSGKTSLSMSLKISKLLNNLVSHTLLFNQSTLLVSSVYALVESLRAVVSAXKPVPSIWSLLDETVARCVRTPYKYIDISTEYGFCSPFMVALLEQFKFVNRDVENYDLLLRWICIFARDLLILGDSSKLRELVAQNISTEKALVATYLNDLDEQNFETLNSEESYLLAQNKTSSFYRFITVSPMKSLSNIQRLPVGMEDIQGALFRLHAVAYNTEIALTKSNCKLVIQNLLEKIGHFMFTSAGLASNFEKIEHYSXFFIQEQDSKIVREKKLYVLVCXLDILLQVRIEVKGLETYLASLFQNYEQINSDFPDIAIFDAIIEYLPVELVDKMSTLELKEVDQAATLRKMXASEEYEANFDYLTKTALSYSSPEITSLVALLLQKCPVKNVKEGLRLFAPLNAKPILQSLALSDREAALEFLXAEILLDNHLMTEALIFVGSFVPDMPQEIGQVLTTTSFSFFQKHPQDVKKVLPLLTQNVAYLTDEQKNTILSYLFTNYPNKYCSEVAKFARFAKDFEKQDFLVWLKKCMLFINKQVAELDSLETMSTNFRAFLADIGSLFEDINVWDVANASVLNSQLEIFFQKKWVFSSFIVNYLSKLLIKESDKIAXSKLFQILINNEHNPLRTKRRVEPIGEKVAMLIFILFNKDPTGNSNFGTQESIVACYGGSLAPHDCIIYEVLQAMESATSTSWASNIYAWDFLDNLNAEESQMLGDTKLIAKEKEGFVVTLNKNVIDFTIENFVVSVPNVPAANAGIAEWNEFTRAFDLFDAHILNSIYDTRFIMLLLLNNTELVQRITNDEDGNVFTRFDMKKILKTQLFSLILISMCSKNEASTISLNIIKQMAQCSNTEESLDTVFKILLKKISFTFIENKDRKPSDFCCPLYWFALTRLCNALLDPRSFMFEKAFRWVFASPNLRYNDIPLFKQLVDFCVHTNDAEDYYKYVIWFLNILECGISSVEDIDFMRKNKIVEWVMNLQTSAYVNDRVYAKVDQVISKVQSVYXKAGVLVSGFAGLSQLEMLTYINDYNLQNITQRHKTNANNLRVYKELLNLKQKQVNEKETALRYGALTSSSKRLREWCNEQDQACIKRVCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBG2</accession>
    <name>A0A1E5RBG2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 16">ADP-ribosylation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g2897</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.</text>
    </comment>
    <comment type="function">
        <text evidence="16">GTP-binding protein involved in protein trafficking; modulates vesicle budding and uncoating within the Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBG2"/>
    <dbReference type="FunCoup" id="A0A1E5RBG2">
        <property type="interactions" value="1185"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBG2"/>
    <dbReference type="InParanoid" id="A0A1E5RBG2"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04150">
        <property type="entry name" value="Arf1_5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003500">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045872">
        <property type="entry name" value="Arf1-5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 13 15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10 16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 16" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 13 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 16" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU369003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ84238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="181" mass="20470" checksum="1AB562EB3123DD7D" modified="2017-01-18" version="1">MGLSFSSLFNGLFGHKEMRILMVGLDGAGKTTVLYKLKLGEVVTTIPTIGFNVETVEYKNISFTVWDVGGQNKIRPLWRHYFRNTEGIIFVVDSNDRARISEARETLQSMLNEDEIRNAVLLVFANKQDLPEAMTAAEITEKLGLQSIRQRQWYIQSTCATSGEGLYEGLEWLSNTLKNQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBH5</accession>
    <name>A0A1E5RBH5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein HOL1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2508</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBH5"/>
    <dbReference type="InParanoid" id="A0A1E5RBH5"/>
    <dbReference type="OrthoDB" id="4500315at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000396">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF4">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="488"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="526"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="568"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="595"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="633"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="663"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="121"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="69"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ84231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="709" mass="79399" checksum="3AB5E6250CD3CDBE" modified="2017-01-18" version="1">MKASDVKKNEDTSVTIEDTSNDTVDHDFNENNAGNAADTLXYHTILGIQQPFYSSVLVKDVPGTYPMLIDDDKTENEPSTTFGDENLDNPVKTKSGIILNPQPTKDKNDPLNWPIWRRDLALIVVGIHCFVGGGQTAVLAAGTTKISQEFNVPLTKVSYLVGLMMVALALGSLIASPTARIVGKRVTYLGGIVVFFFGSIGCACSNNYPALLISRIVSGFGVSTVESLPSATIAEIYYAHERAYRFGIYTLLLLGGKNLVPLFGSLVFQNLNIHWLFWIITIIVGIDFILHVLFVPETFWNRAPVPSKRSLKESQFAREQGYKPPIGDYDDDEELSQEQQAQFLENMKLTVHFVSPTKKKTRIDNGNEIVLDTQKHLNSDSTKKDNEDAQDDTAVTIHSFGPEYNETDGTPHQLFHMDHEKEFNTXKDVESSLEHNDTQSTFVGKSRRGSNATSVLTSGLSLYNGRHSKDKWTHIFLRPFVLFCYPHVLFCSFLYCFSVVWLIVIAQLVAHIFMDKTYNFTSLETGLIYISPFIGGCLGSLCAGTVSDYVVRFMSKRNHGFYEPEFRLLMCIPAVLATAAGMFAFGWSAQLHDMWFVPTFFFGLLAFGCSLASTTAITLTIDSYKNYAQESLVTFNVCKNIMGFIFSFFNTDFAQNSGTKTTFIVYGCVELALGLCXIPLYIYGKRIRDWTHKANLMEFTYKQISEENH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBJ6</accession>
    <name>A0A1E5RBJ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2464</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RBJ6"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="386"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="496"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="427"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="496"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="513"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="528"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="457"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="538" mass="60912" checksum="B6EE7938DB60473E" modified="2017-01-18" version="1">MGATSIDEGALIDFLLSGSLETNISAFLPLEPVLLPSAEVTCKEELFCILGPKNHLESTQNKGKSSMKRKKQATATKTNAFDDKFLCDLIKIVMEPPPKIGKKSLTSRKNNSKISVSGIDSSKDAKKXKKSNGFFSDYEAVFEKQSVKRNEKRQRKEKHQNNKTDRFDEMPQSNEDYSAKHVENERFGNRTEQTHYIRESFKRTKSQKEKDLNSKKKERKQQAGRAATSVEQCLPQTDKSDRLSMKGLDKQSAENKVISQDDKLLNKKSQKKDKKKQXRAARREPFEDPSSFETQRVIGTNNSKSSVEMPRKLKNNGKDNESLMEAQVTDSFSSXXKKTRKNKKKNKTKNTESKYPLASEKXAEQISFYHTEEAFLDEPNEFASDRTLSFESITSPKSKKKKSKKSSKKTSKDAXSSFPNPLEFAPNSKNFSLSEPQLQKSKAKKGGRSNSSFGKDDSMGDNLVLTNDEIEEILIEACVYDNFYQGSLAEQICAEEQSQCGPMTNTXINTRASKSKKKKNKRKNNKSKTQDSTEKDXQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBJ7</accession>
    <name>A0A1E5RBJ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">T-complex protein 1 subunit epsilon</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">CCT-epsilon</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2804</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBJ7">
        <property type="interactions" value="1371"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBJ7"/>
    <dbReference type="InParanoid" id="A0A1E5RBJ7"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03339">
        <property type="entry name" value="TCP1_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000003">
        <property type="entry name" value="T-complex protein 1 subunit epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012718">
        <property type="entry name" value="Chap_CCT_epsi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02343">
        <property type="entry name" value="chap_CCT_epsi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ83943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="558" mass="61636" checksum="4312011FA88E13CB" modified="2017-01-18" version="1">MAHHQPQSMPDMSNAIMAQDEMGRPFIIVRDQSSKKKSSGLEAKKSHILAARAVSNIIKTSLGPRGLDKILISPDGDITITNDGATILSQMELDNEIAKLLVELSKSQDDEIGDGTTGVVVLACALLDQALDLIEKGIHPIKIANGFDEACKIAIAHLEKISEDLFDDKEKFEKVLFKVAKTSLGSKIVSKEHDQFADMAVKSIMSVVNWERKDVDFDLIKLEGRVGGSLKDSKLINGVILDKDFSHPQMPNEVNPKDGESNVKLAILTCPFEPPKPKTKHKLDISTVEEYKKLQDYEQDKFKEMIEFVKNSGADVVICQWGFDDEANHLLLQNNLPAVRWVGGQELELIAIATGGRIVPRFQDLSADKLGKCSRVYEVEFGTXREKMLVIENSEESKSVTCFIRGSNKMIVDEAKRALHDSLCAVRNLCRDSRVVYGGGAAETTMSLAVSEEADRQRGIDQYAFRAFANALDIIPMTLAENSGLDSIETLSNIKSTQVKEGLSHIGVDCLGKGSNDMKELFVVDPYVGKKQQILLATQLCRMILKIDNVIISGKDEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBL7</accession>
    <name>A0A1E5RBL7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aspartate aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBL7"/>
    <dbReference type="STRING" id="56408.A0A1E5RBL7"/>
    <dbReference type="InParanoid" id="A0A1E5RBL7"/>
    <dbReference type="OrthoDB" id="7042322at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43510">
        <property type="entry name" value="AMINOTRANSFERASE FUNCTION, HYPOTHETICAL (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43510:SF1">
        <property type="entry name" value="AMINOTRANSFERASE FUNCTION, HYPOTHETICAL (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="1">
        <location>
            <begin position="48"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="380" mass="42761" checksum="3D9F5E8A37D86017" modified="2017-01-18" version="1">MVLEEGFGVEEFTHKYETKVDYNLAEACCYSLSLNDVYKLTGEKFEINNDSRFTYGAIDGTDNLRGLVADLYSDEKVKFTKDNVLITNGGIGANFLVYYTLFGKGDHVICVSPTYQQLFSVPKMFGADVELLRLQKENGFVPSVEQFKKLIKPNTKAFILNNPNNPIGSVMETDLLEEITKVAQEHGITIISDEVYRPLFHSSDRVPKSVAQLSFDGISTGSMSKAFAFAGVRLGWIVSQNTDFLKAARSKRHYNIISISMIDDQISQYVLQNKKKILQKNKQLCLKNLDYLSQWVKINEKVEFLDFPKGGSVCLIKIDGISDTQKFATWLAENKSFLVVPGETFQCPGTLRLGYANSEDDVAKGLEILSKSIDEYKEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBM9</accession>
    <name>A0A1E5RBM9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Replication factor C subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2799</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBM9"/>
    <dbReference type="FunCoup" id="A0A1E5RBM9">
        <property type="interactions" value="625"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBM9"/>
    <dbReference type="InParanoid" id="A0A1E5RBM9"/>
    <dbReference type="OrthoDB" id="4199794at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031391">
        <property type="term" value="C:Elg1 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000011">
        <property type="entry name" value="Replication factor C subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000237">
        <property type="entry name" value="replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF20">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="51"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ84310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="353" mass="39782" checksum="EF3700B97445198C" modified="2017-01-18" version="1">MSQQPLLKKQKFSAPVENKPWVEKYRPANLDEITAQDHAVQVLKSSLRTANLPHMLFYGPPGTGKTSTILALTKELFGPRLMKTRVLELNASDERGISIVREKVKSFAGLTVSKPSKEDLENYACPPYKIVILDEADSMTADAQAALRRTMETYSNVTRFCLICNYVTRIIDPLASRCSKFRFKPLDSFNALSRLEYIAEQEHVMFSKNSKTQVCTKILNICDGDLRKAITLLQSASKVNKQQDSDGLTEAQIEELAGVIPENILNELISVIKTKSANKIIAYIKDFMNQGYSVSTVVSQLHNYYFVSEKASDLKNNNEIDWLLFETDSKLTNGTNEHIQFLNLALNLAQQML</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBN4</accession>
    <name>A0A1E5RBN4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">aspartyl aminopeptidase</fullName>
            <ecNumber evidence="4">3.4.11.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2707</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal aspartate or glutamate from a peptide, with a preference for aspartate.</text>
            <dbReference type="EC" id="3.4.11.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the peptidase M18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.21" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBN4">
        <property type="interactions" value="602"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBN4"/>
    <dbReference type="InParanoid" id="A0A1E5RBN4"/>
    <dbReference type="OrthoDB" id="9880441at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05658">
        <property type="entry name" value="M18_DAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.250.10:FF:000001">
        <property type="entry name" value="Aspartyl aminopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.250.10">
        <property type="entry name" value="Aminopeptidase i, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001948">
        <property type="entry name" value="Peptidase_M18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023358">
        <property type="entry name" value="Peptidase_M18_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002759">
        <property type="entry name" value="PRK02813.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570">
        <property type="entry name" value="ASPARTYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570:SF3">
        <property type="entry name" value="ASPARTYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02127">
        <property type="entry name" value="Peptidase_M18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00932">
        <property type="entry name" value="AMINO1PTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101821">
        <property type="entry name" value="Aminopeptidase/glucanase lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11 12" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="8 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6 11" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ84302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="489" mass="53769" checksum="DD614580C768E656" modified="2017-01-18" version="1">MTTSTSSTYPAEFIDFLNATPTPYHAVENIKNKLKSNGFVEILERDVWHGKVQKLGKYYVTRNNSSIAAFAVGGKWSPGNPIAVTGAHTDSPVLRIKPISKKTNEGFLQVGVECYGGGIWHSWFDSDLSLAGRVMCANNNTGVVEAKLLNLQKPLLKIPTLAIHLDRSVNESFKFNKXTHLLPIASLQSKSKETQNKNGCCSSKENELNEQEFNCVETIIKRHHVDLLELIVKELDLSSIDCIDDFELILYDNKPSTLGGLHDEFVFSGRLDNLTSCFTSTWAMAESCDTNLEKDTGIRLMACFDHEEIGSVSAQGAESTFLPNLLERLNGLKFDDTDVSSPLARSLVMESAAKSFFLSSDVSHGVHPNYASNHEKSHKPMLGAGTVIKINASQRYMTNAPGLTLVKKLANAAKVPIQLFVVANDSPCGSTIGPALAAHTGIRTLDLGNPILSMHSIRETGGADDLEHQIKLFKKFWEDYSTLEEKIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBP4</accession>
    <name>A0A1E5RBP4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Peroxisomal membrane protein PMP47A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2679</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBP4"/>
    <dbReference type="InParanoid" id="A0A1E5RBP4"/>
    <dbReference type="OrthoDB" id="2019556at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015217">
        <property type="term" value="F:ADP transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080122">
        <property type="term" value="F:AMP transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005347">
        <property type="term" value="F:ATP transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015228">
        <property type="term" value="F:coenzyme A transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015230">
        <property type="term" value="F:FAD transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044610">
        <property type="term" value="F:FMN transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051724">
        <property type="term" value="F:NAD transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052217">
        <property type="entry name" value="Mito/Peroxisomal_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45939:SF5">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PMP34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45939">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PMP34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="104"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="197"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ84318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="309" mass="34276" checksum="862458B3E72993A2" modified="2017-01-18" version="1">MSSLEHALAGATGGALSISLTYPLLTVTTNLQTKEEVLGNKPRSVKXVFLDLWETKSLYKGLESSLYGITLTNFIYYFFYEYCSKILLTRKLNKKNNAQKSQKMNTIESMFXGFIAGTITATLTNPVWVANTKLTTSTSLDKKASTYQAIVQIVKEXGFLQLFKGLKPALVLVINPIIQYTVFEQLKNTVALSRDSLSPAMGFVLGAVSKLTATGITYPYITLKTRMHLESNGADGKSTDMMTMLRDIIQQEGLWSGLYRGVTVKLVQSVLTAAFLFFFKEGIVQGIVKLTHLKKQSRLVSTISRKKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBR5</accession>
    <name>A0A1E5RBR5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 19">Serine/threonine-protein kinase RIO1</fullName>
            <ecNumber evidence="4 19">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">AWRI3579_g2699</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 22">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4 19"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBR5"/>
    <dbReference type="FunCoup" id="A0A1E5RBR5">
        <property type="interactions" value="1040"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBR5"/>
    <dbReference type="InParanoid" id="A0A1E5RBR5"/>
    <dbReference type="OrthoDB" id="205248at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000755">
        <property type="entry name" value="Homoserine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051272">
        <property type="entry name" value="RIO-type_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018935">
        <property type="entry name" value="RIO_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017407">
        <property type="entry name" value="Ser/Thr_kinase_Rio1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45723">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038147">
        <property type="entry name" value="Ser/Thr_PK_RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01245">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 19 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 19 25" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 19 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 19" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RIO kinase" evidence="24">
        <location>
            <begin position="58"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="108"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="439"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="23">
        <location>
            <begin position="446"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="483"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="23">
        <location>
            <begin position="511"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="20">
        <location>
            <position position="294"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="20">
        <location>
            <position position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR038147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR038147-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR038147-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR038147-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="SMART" id="SM00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="OEJ84326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="535" mass="62388" checksum="EA67BFF167F111A6" modified="2017-01-18" version="1">MPQMDDYDDGFSKMKISKAKNSHMQDHTEHDKSKIMTKFADKIKTDEMSYNRAKVSKDKADRATVENVLDPRTMRFLKSLVHRGVLYNGINGCLSTGKEANVYHAFEGDRTSDERRNHNRFDDSSKDNNNNSVPSLVHTKEEAIETPLSKRREFAVKIYKTSILVFKDREKYVEGEFRFRHSRSQHNPRKMIKVWAEKEFRNLKRIYQSNIIPCPEPIEVKSNVLVMEFLTDSKDIANGDGIEAPENMGTGNSRPSPKLRDHNFQDDEDIKHYYYTMLAYIRILYQKCALVHADLSEYNSIVHNGVLYIIDVSQSVQPEHPMSLDFLRMDIKNVNGFFERMGIPICCERLIFQFVIQEVLPNCENDLDVEILRNYARENIRIKETQEDDEEDQVFRSLYLVRNLNGLEERDFERFTDGKFDLLKSLLASDNAKVQKLQKNNHGLNDDNDDENSDSDEGSSSEDSDEEGSEEEENSDEQDSDWEDRSVDLKGKKFEDKDAKKDRKEATKLAKREKRATKMKKHVKKKLVNKTKTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBS2</accession>
    <name>A0A1E5RBS2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">High osmolarity signaling protein SHO1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">High osmolarity signaling protein sho1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11 12">Osmosensor SHO1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g3035</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SHO1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBS2"/>
    <dbReference type="FunCoup" id="A0A1E5RBS2">
        <property type="interactions" value="192"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBS2"/>
    <dbReference type="InParanoid" id="A0A1E5RBS2"/>
    <dbReference type="OrthoDB" id="5983572at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007232">
        <property type="term" value="P:osmosensory signaling pathway via Sho1 osmosensor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11855">
        <property type="entry name" value="SH3_Sho1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000213">
        <property type="entry name" value="High osmolarity signaling protein SHO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035522">
        <property type="entry name" value="Sho1_SH3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735">
        <property type="entry name" value="FCH AND DOUBLE SH3 DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735:SF20">
        <property type="entry name" value="HIGH OSMOLARITY SIGNALING PROTEIN SHO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="9" id="KW-0346">Stress response</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="382"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="204"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="286"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="207"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="294"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="319"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="330"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ84342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="442" mass="49439" checksum="D610DE8E8098A0FB" modified="2017-01-18" version="1">MSEKLYSYQLRKKNSSNYSRKHFTFRISRFFGDPFALTTLSLCIVSFVVALVGSITAATDNEGFPKFTWWGLAYQFLMMVSIFFLYCFDLVDYYKNFLTAALGVAFVYNTNSTTILIYADGSRKAAASAGCIMLSIVNLIWMFYFGSDNGSPLNRKIDSYSLNGMKMSNLMSEYAQDRRYSSLNRNSRSNIYRNSYADYSQRHTSFQAPPAQQQQQQQQQELQHLQHSQASAYQDQQQQQQQQQNQPFAHNNYMSSTALNGFENIDSSNQTTPQLGNTLNRLQNDTLTTSHNEGTGTTNITGNSNTTGNTGITVNSGTPGNTVTTGAMATTENTGNNTPSTNNTRFVNPATARADKSKSSITDSTAPESMGLYSDMAEELSQFPYKAKALYSYEADPSDAYEISFSEGEILSVADIEGRWWSCRKENGERGIIPSNYVELIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBS5</accession>
    <name>A0A1E5RBS5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Transketolase</fullName>
            <ecNumber evidence="4 15">2.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g2810</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 15">
            <text>D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = aldehydo-D-ribose 5-phosphate + D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:10508"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="15">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <cofactor evidence="15">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="15">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <text evidence="15">Binds 1 Mg(2+) ion per subunit. Can also utilize other divalent metal cations, such as Ca(2+), Mn(2+) and Co(2+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13">Binds 1 Mg(2+) ion per subunit. Can also utilize other divalent metal cations, such as Ca(2+), Mn(2+) and Co(2+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="12">Binds 1 thiamine pyrophosphate per subunit. During the reaction, the substrate forms a covalent intermediate with the cofactor.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 15">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the transketolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.1" evidence="4 15"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBS5"/>
    <dbReference type="FunCoup" id="A0A1E5RBS5">
        <property type="interactions" value="738"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBS5"/>
    <dbReference type="InParanoid" id="A0A1E5RBS5"/>
    <dbReference type="OrthoDB" id="10267175at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004802">
        <property type="term" value="F:transketolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07033">
        <property type="entry name" value="TPP_PYR_DXS_TK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02012">
        <property type="entry name" value="TPP_TK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000003">
        <property type="entry name" value="Transketolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000003">
        <property type="entry name" value="Transketolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000004">
        <property type="entry name" value="Transketolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055152">
        <property type="entry name" value="Transketolase-like_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005478">
        <property type="entry name" value="Transketolase_bac-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020826">
        <property type="entry name" value="Transketolase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049557">
        <property type="entry name" value="Transketolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033247">
        <property type="entry name" value="Transketolase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00232">
        <property type="entry name" value="tktlase_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43522">
        <property type="entry name" value="TRANSKETOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43522:SF2">
        <property type="entry name" value="TRANSKETOLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22613">
        <property type="entry name" value="Transketolase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00456">
        <property type="entry name" value="Transketolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00801">
        <property type="entry name" value="TRANSKETOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00802">
        <property type="entry name" value="TRANSKETOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 13 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 13 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12 15" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="16">
        <location>
            <begin position="357"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="419"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="117"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="387"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="446"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="470"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="478"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="482"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="529"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="14">
        <location>
            <position position="30"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="14">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605478-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605478-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR605478-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR605478-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR605478-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ84354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="682" mass="74004" checksum="2F122A80B5A2B75D" modified="2017-01-18" version="1">MSKFTEIDQLAVNTIRVLSVDQVSAANSGHPGAPLGLAPAAHVIYKNMVFNPKNPEWINRDRFVLSNGHACALLYSMLHLTGYDAFTLDDLKQFRQLNSKCPGHPEFELPGVEVTTGPLGQGISNAVGLAIAQANFAATYNKPGFELSNNYTYAMLGDGCLQEGVSSEASSLAGHLQLGNLIAFYDDNKITIDGATNVSFTEDVLKRYEAYGWEVLTVNGGNDDLDAISNALEQAKLTTDKPTLIKLETTIGYGSLNQGTSGVHGSPLKPDDVKQLKKRFGFNPEEKFVVPQEVYDFYNQHIIVPGKKANAEWDALFAKYTEQYQVEGATIARRLRGELPQGWESKLPLYTPKDSAVASRKLSEIALDAIFDDIPELIGGSADLTPSNLTRSKDATDFQPPATGLGTYAGRYIRYGVREHGMGAIMNGISAYGANYKPYGGTFLNFVSYASGAVRLSALSGHPVIWVATHDSIGLGEDGPTHQPIETLAHFRAMPNIQVWRPADGNEVAAAYKQALSSRHTPSIIALSRQNLPQLEGSSIEKASKGGYVLQENKDAKITLVSTGSEVGIVVDAVKVLASKGISARIVSLPDFHTFDQQTQEYRLSVFPDGIPVMSVEVLSPFGWSKYADESFGLNRFGASGKGPEVYQFFGFTPEGIAQKAEKTVAYYEGKSVYSRIHQASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBV9</accession>
    <name>A0A1E5RBV9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Altered inheritance of mitochondria protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">AIM11</name>
        <name evidence="5" type="ORF">AWRI3579_g2984</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the AIM11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBV9">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBV9"/>
    <dbReference type="InParanoid" id="A0A1E5RBV9"/>
    <dbReference type="OrthoDB" id="4088121at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038814">
        <property type="entry name" value="AIM11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39136">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39136:SF1">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ84371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="159" mass="18247" checksum="1AD8806AD9A6DFDC" modified="2017-01-18" version="1">MLLXPAQITKSQLEKFKNEYKERRRVQMLRFFGASIFTLITARLAHRSIQSRKYVPKMFENNYKVPPFSYQYESISALLYGSAFSIGGFGMFVTGACWMNNISTFPEFSKTLKLALLGEEGVEELNKQMNALNSQPLDEDTKGVLEVIENAWSNNNKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBW3</accession>
    <name>A0A1E5RBW3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Lactose permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2851</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBW3"/>
    <dbReference type="InParanoid" id="A0A1E5RBW3"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000134">
        <property type="entry name" value="MFS sugar transporter protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF64">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="375"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="413"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="442"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="475"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="514"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="544"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="110"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ84382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="617" mass="69022" checksum="0DCB571CFE11F9C1" modified="2017-01-18" version="1">MTLFVKNQSQTSFEKNDNSYSASIDTPEQLGKETPQYRDRNGISSSSSSSSSSSNNISNDNRNNNIYDDASNKDSTVNNGNMHLNIETVMPSFDGKKWYQLAHIRQLCFTIFCISLTSCNNGYDGSLLNSLYTEKDFNNAIGNVSGSILGCLTSGFQFGNILAFGVAAMAVDNLGRIKALNVGNAIMLLGTLIQGLSGVWIRGLPQNYQGRDVLGMLIASRIVLGFGTGIISVAAPSLISELSFPTHRQTTTTFYNSSWYLGAIIAAWVSFGVKDVPHHWSWRIPTIVQGFAPLAQIILIPMFVPESPRWLVXKNRVEEAREILDKYHGGGYEGSDMLVDYEMTEIQLSIEQERAASSSASYKDFFKTKPNMKRLWILVWTAIFMQLSGNGLVSYYLGKVLNSIGYTSTSQQLIINAGLMIFNYGVSLIQSLLIIPKITRRAAFNTSVFGMLICYIIWTILSAINQQRDFKDQSLGQGVLAMIFIYYFFYNFGMNGIPFTYCTEILPFTMRGRGMNIFTFSQYIVQIYNGFVNSIAMDAIEWKYYIVYCCILAVECCVCYFTFVETSGRTLEEVADVFGDGIQDLGNVSGITALEDGKLKHNEEFEHKEYAMDEENY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDI0</accession>
    <name>A0A1E5RDI0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="11">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g2018</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="11"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDI0">
        <property type="interactions" value="456"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDI0"/>
    <dbReference type="InParanoid" id="A0A1E5RDI0"/>
    <dbReference type="OrthoDB" id="3972275at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031422">
        <property type="term" value="C:RecQ family helicase-topoisomerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000729">
        <property type="term" value="P:DNA double-strand break processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17920">
        <property type="entry name" value="DEXHc_RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18794">
        <property type="entry name" value="SF2_C_RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000296">
        <property type="entry name" value="ATP-dependent DNA helicase RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000340">
        <property type="entry name" value="Bloom syndrome, RecQ helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004589">
        <property type="entry name" value="DNA_helicase_ATP-dep_RecQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032284">
        <property type="entry name" value="RecQ_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018982">
        <property type="entry name" value="RQC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00614">
        <property type="entry name" value="recQ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF153">
        <property type="entry name" value="RECQ-LIKE DNA HELICASE BLM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16124">
        <property type="entry name" value="RecQ_Zn_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09382">
        <property type="entry name" value="RQC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00956">
        <property type="entry name" value="RQC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="760"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="959"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="54"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="170"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="539"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="698"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1262"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="56"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="117"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="170"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="191"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="543"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1264"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1288"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1307"/>
            <end position="1330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ84958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1330" mass="149901" checksum="8DCC3E665C5AD2E9" modified="2017-01-18" version="1">MSTHDPLEKPKKASHHVRKELNWLKKTQAEKPNQELLLNIFKLSKNVELRQEPNFSSNLSTRTSNASSTSLNATPPAVIQSKPVSSSSSPTVSVEPVLPPNSRVFERPTSNTTVRTINSTNSLISNSKNSSSKGNSISSINESKPKPFVNLTRPPAKNVVDFFSQNKSVPQTPRFNANGNIELPLSRGTPKASNNISNNLSMRGKTKEFNNISAQVLLPNMQQPKLLEQLQSRTSSAERSKLFNDRISNFVYDGETSTSLSEHYDKNTENGKGVTSFADRNXEANNVVTIMTPLEKQALDKSNSLLNSISGFIDLTEDVPTKKRKISETPIATPTGATNNTEQSTLEKVAIKDKLIAALTEQNELLLKKCSILQSTSLSEDHKRKQIKYNVDPLIETQSLEISTLQKQVVSSDIQKENFIDSTVQSPPLQIPTTSKSVYSYPEELHAATSNTNFNNHIEDSFAESESDLEELVNQCKSAQYMPPNSTATFDSISQXPLSSAPFDHQQPLLQSSAAVSKKSNLSDITDEAEYEDSNYFNSGIEEDTRSSDRGFIDDNGNTNISMDEDASYKEEEEEEEVNLDRIQDNNDENHSSLVEIIDSSPLKRIVDYQHQSDTEMSLSENDFELLMQKNNHTENGSDEEDPSDIDDLMELENDVFDIEREHFTQNIKDVDDDLKIISETKLTEEQLDHAAEVAIKREAEARQKPPQTSNSDLGLEEEDDDEDILNMAQPSYPWTDEVNTQLHQVFKLSSFRANQLDAINGILGGRDVFVLMPTGGGKSLCYQLPAVVKRGQLHLTTIVISPLISLMQDQVEHLRKKNIRAGMISSKNTTEDRRHMFNLFIDGLLDLVYLSPEMVSASQQCKRAIDKLHREQKLARIVVDEAHCVSNWGHDFRPDYKELKWFKEMYPTVPIMALTATASEQVRMDIVHNLKLKDPLLLKQSFNRSNLIYKVAMKNKNTIFEMAKEIKTKFKNMTGIIYCNSKNQCEKTSQFLSTQGVSCSYYHAGMENDDRSRVQRQWQNDELKVVCATVAFGMGIDKPNVRFVYHFTLPRSLEGYYQECGRGGRDGKTSYCTLYFNYADFKSIESLVKRDKDLSKDNREKHLSKLNDVLQYCNNTIECRRKLVLSYFNEAFDPKTCRKTCDNCRANNLVTQKDITKEAKDIIALVGALDGQQVTASYCMDVFRGSKSSKIVTAGHDNLPQHGLGKAYRKDDLQRIFQNLISAKYIGEYTKFNKSGFASTYMKRGTRALKDKVLMNFALNXPSSSGSKSSTRLSSAAAPLDKEEQKAMQQTYKKIASQSQANSYGNKRKKGYRGAKSSKKYYKKRSRTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDI1</accession>
    <name>A0A1E5RDI1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2339</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RDI1"/>
    <dbReference type="OrthoDB" id="3972751at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="118"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="345"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="583"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="376"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="407"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="419"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="583"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="627"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="652" mass="71888" checksum="45623AA2E27CC136" modified="2017-01-18" version="1">MITSPTLPPLQQHMNNSGAMPGMSMENTNGTYNKNGIKSNDPSNFTNSXXFLSTPMTSPTSSVMPNTVTTNNKHNHSQDNSTLDHHIVDHNHSDSSSTHAHDHELHIHSSPKKRVSKACYRCRQSKTRCININGGVCERCINENELCIYSKPPNPSTTSHSNKKSLKETYQQGKIKKLYNQEYINLLENKVAIYELFTKQIFNNFNNLIGSNVLQMHKENIVHAKLNEHNSETRSLEHDHDKKIDESLEIWNKLFENKDYFCSNEIIKTTNNVLTQHPTNVNINQLIYLILPQNMDLAVKSINYIADEHRDANLIFNTKTTSNLNLDHLNDGYVNKHNFFQNDLHKPVIEQPNKPQSSNKPQRKRRRTTIHSLPHPNIGSGSTSSXNNSGLGPLNGGSSITLPFLANPNSGTMSTRSITPTNNDDPTTSPHVKFTNGGTVLQPLNTHFIHSSNSSSSNGMIPPHQQLSSPHTYMSLPHLQPGPLFAQQQQFQLSKNQPKLNSLXSSISYTPSSSFPNILDSKMHLSSNNTSSYGSSNSLHFGNILGPNNNHMGSMPLLPSTMTPSASTSNLLNNGLLNSAGAMSSTSNGFPTANTNTLMKKSEDSNSVSPSSGNTPNLLPKMVFPLSNNTTQSGSTDTTTNPPPLANGLQSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDI2</accession>
    <name>A0A1E5RDI2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2448</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDI2"/>
    <dbReference type="FunCoup" id="A0A1E5RDI2">
        <property type="interactions" value="160"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDI2"/>
    <dbReference type="InParanoid" id="A0A1E5RDI2"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF803">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="10">
        <location>
            <begin position="91"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ84944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="421" mass="48149" checksum="A87666941E54389D" modified="2017-01-18" version="1">MGQIASHPLTDKIIYYEPYELKNQAVSTRQLPTQFFNCLGTMQGYRLTQEDSHLICNGKFSNSANANSVISKITKSDESNNAQKMNKNNDIFHKNEIQVKFRNPLNNDKYEVMWLSLFGVFDGHGGDKISKALSYKLNDSIWKTLEHHTYTKHSFRTVQGLLSQLFKDSFIKLDKEFFKTYKYSTQCGSTAVVCIIVNGKTIYCCNSGDSRALLSLKPTRASKLHSAHSNSTANEKHHHSSVATNLCCVKNLSFDHKPLHIGELMRINDNGGTVSLGRVNGVLALSRAFGDFQFKRHTYNHHKHKHGIPQEEQVTCEPDVLIHDINYEKDEFLVLACDGIWDVYSNKRLTQFIRYHLCLGLKLDAIVKKVLDHGIECADPETGCGFDNYSIIIVALNKENESVEEWYERMKMRLLRERRIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE73</accession>
    <name>A0A1E5RE73_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Large subunit GTPase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2051</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RE73"/>
    <dbReference type="FunCoup" id="A0A1E5RE73">
        <property type="interactions" value="1276"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE73"/>
    <dbReference type="InParanoid" id="A0A1E5RE73"/>
    <dbReference type="OrthoDB" id="61815at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01857">
        <property type="entry name" value="HSR1_MMR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001151">
        <property type="entry name" value="Large subunit GTPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043358">
        <property type="entry name" value="GNL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45709:SF2">
        <property type="entry name" value="LARGE SUBUNIT GTPASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45709">
        <property type="entry name" value="LARGE SUBUNIT GTPASE 1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="7">
        <location>
            <begin position="189"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="619"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="624"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ85201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="646" mass="74097" checksum="1739CE0BDCE2B70E" modified="2017-01-18" version="1">MPPKRPEPKKWKPPKGPKPIQRKNKNTIGLGKAIITQRKKENQVNILPDGEMHFTTDKHEANWVKLRSVTQESALDDFLSTAELADKDFTADRHSNVQIIRMDQNTDNVRSQGFMMTNEAKSELNAKQREHLKDLIVPRRPHWTKEMTAYELQRLEKEEFLTWRRKIASLQESNEDLLLTPFERNIEVWRQLWRVVERSDLVVQIVDARDPLLFRSVDLEKYVKEIDDRKQNLLLVNKADLLTVKQRISWAKYFISRNISFSFFSAKKANEILEDQLEFGDDYIAKKDYYSEINNILDEEEVDPELLGKIKILEIDQLEELFLSNAPEEPLLTPLPGQESLIQIGLVGYPNVGKSSTINALVGSKKVSVSATPGKTKHFQTIKLSDEVMLCDCPGLVFPNFAYNKGELVCNGVLPIDQLRDYIGPCTLVVERVPKYYLEAIYGIHIQTRSVEEGGDGETPTAQEFLVSYARARGYMTQGFGSADESRASRYILKDYVNGKLLYINPPPHMEDDTPYTKEECKEFNKELYTFDHLPENRKEQLSEALKSKNIEDFDLSRDLSKLTFSAHVGAEGDNTAQSVNHGGKQAALYNAAHDLDKEFFNMNSVEGKFLSPFHSKNIEAGSSRKKHNKKNKKAKKKAEMLYSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE93</accession>
    <name>A0A1E5RE93_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transcriptional regulatory protein SAP30</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1690</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAP30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RE93"/>
    <dbReference type="FunCoup" id="A0A1E5RE93">
        <property type="interactions" value="132"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE93"/>
    <dbReference type="InParanoid" id="A0A1E5RE93"/>
    <dbReference type="OrthoDB" id="3973244at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024145">
        <property type="entry name" value="His_deAcase_SAP30/SAP30L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038291">
        <property type="entry name" value="SAP30_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025718">
        <property type="entry name" value="SAP30_Sin3-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13286:SF6">
        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT SAP30L-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13286">
        <property type="entry name" value="SAP30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13867">
        <property type="entry name" value="SAP30_Sin3_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase complex subunit SAP30 Sin3 binding" evidence="8">
        <location>
            <begin position="180"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="137"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="222" mass="24855" checksum="C579FF27F3780083" modified="2017-01-18" version="1">MPPGRPSNSSRDHHTGNNNGNHGGSNKKHSNENDSHQSKKSGNAGGHGSSKSTHAQQAYLKDLIATHVTNNHPDLQDRPHPLDFESYSSDFLRRYKDKYELPIRSDNMSLQGYLLGSKLGAKTFSWKKINHNSVRKPQIRGRASVSSGSSNSSRDSEVQEEQNVASSMTLTDEIIKGKRVSKKVLADSVKAHFLTSNIKESECIPQFIYKIKNDKMKFKMEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REI4</accession>
    <name>A0A1E5REI4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alcohol dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2435</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10736"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REI4">
        <property type="interactions" value="1046"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REI4"/>
    <dbReference type="InParanoid" id="A0A1E5REI4"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000947">
        <property type="term" value="P:amino acid catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08297">
        <property type="entry name" value="CAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000039">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000002">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF3">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="10">
        <location>
            <begin position="82"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="11">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="416" mass="44408" checksum="238D282AAB018ACA" modified="2017-01-18" version="1">MLKAFASVKMTLPLAQKNLGVVKPFXYRPGSKFGMNQASGIFFFRATYKTSSSSNTTFTASTSSTTPSIPKKQKGIIFYKNGGELKYGDIAVPKPKPNELLIKVLFSGVCHTDLHAWKGDWPLPVKFDPYLVGGHEGAGIVVGMGSQVKNWKVGDAAGIKWLNGSCMSCESCELGHESNCMQADLSGYTHNGTFQQYATADAIQAARLSPNVDFAQVAPILCAGVTVYKALKSANLNPGQWVAVSGASGGLGSLAVQYARAMGLRVLGIDGGEGKEKLFYECGGETFIDFTKEKNVSEAIIRATAGGPHAVINVSVSEAAISESTKYVRTNGTVVLVGLPAHAVCKSEIFSHVVKSIKIVGSYVGNRADTREALDFFDRGLXKTPINLIGLSELPTAFEKMESGKILGRYVVDTSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REQ8</accession>
    <name>A0A1E5REQ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ras-related protein SEC4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g2421</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in exocytosis. Maybe by regulating the binding and fusion of secretory vesicles with the cell surface. The GTP-bound form of SEC4 may interact with an effector, thereby stimulating its activity and leading to exocytotic fusion. SEC4 may be an upstream activator of the 19.5S SEC8/SEC15 particle. SEC4 probably interacts directly with SEC8; it could serve as the attachment site for the SEC8/SEC15 particle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Cytoplasmic vesicle</location>
            <location evidence="14">Secretory vesicle membrane</location>
            <topology evidence="14">Lipid-anchor</topology>
            <orientation evidence="14">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REQ8"/>
    <dbReference type="FunCoup" id="A0A1E5REQ8">
        <property type="interactions" value="571"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REQ8"/>
    <dbReference type="InParanoid" id="A0A1E5REQ8"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000961">
        <property type="entry name" value="Ras-related protein Rab-8B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="188"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="188"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="212"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ85398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="222" mass="24291" checksum="CB7CE08138DEAD36" modified="2017-01-18" version="1">MSGAAVPSSTTSSSFDSILKILLIGDSGVGKSCLLVRFVEDNFMPTFITTIGIDFKIKTIEINGKKVKLQLWDTAGQERFRTITTAYYRGAMGIILVYDVTDERTFANIRDSWYNTVSENASSECQLMLVANKCDEGDENRVVTKEQGQTLAKELGMAFVEASAKDNKNVDEIFFTLAKLIQDKTDANNAANGQSGNGSNRQHQSEGINIKKGQSGSKNNCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REV9</accession>
    <name>A0A1E5REV9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1733</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REV9">
        <property type="interactions" value="106"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REV9"/>
    <dbReference type="InParanoid" id="A0A1E5REV9"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000145">
        <property type="entry name" value="probable ATP-dependent RNA helicase DHX40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049945">
        <property type="entry name" value="AAA_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF83">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ATP-DEPENDENT RNA HELICASE DHX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="164"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="365"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="825" mass="93041" checksum="64DBF5E22A1B9CB9" modified="2017-01-18" version="1">MCAQRQDSHPRNNQQQRFQXGQETRNGNSHRTRYQQDGSNREXHLPYNQLKSHTTGNALSNTKNKNKQGVWESNQLKQAYASTTQVSETAAQNGGSALSNPDEIFVPGAQDYEFVFDENVHVDYDSGEMEEPLADNEEENTDDTDETQVENNLLPITKYKEKLLRAVKDNQVLIVVGETGSGKTTQLPKYLMQDLNYRVAVTQPRRVAATSVAARVAQEVGVPLGGKVGYSIRFDDKSDGLHTRLKYMTDGMLLREFVAKSELSEYDCIMIDEAHERTLATDILLGLLKERVLGTGAGEEEAADKRNTHRKRTKIIISSATINSERFSQYFNNAPIFHIPGKTFPVNIHYVLQPEANYVQASITTIFQIHLAQQRECKGDILVFLTGQEEIRLVQNSIEQICEKLNVSNMIVAPIFASLSNDQQLKIFRETPVNCRKVVLATNIAETSLTIDGIKYVIDPGYVKVKNYNPLTGMSELVVEQCSKASVNQRAGRAGRTSSGDCFRIFPKNTYENEMAELPEPEIKRSNLSSVVLLLISLGVKNLLKFELLDKPSVPALGAALDNLYMLGALNSKGQITNLGTIMNIMPCEPNFVKVMMVASQKFHCLNEILKVIAMLQETQNIFSELAGDSKKTDNKKNXDVSVFTNPEICESNGPIHSDHYLYLKIFEAWEKNKMSKAWCRNNKLQWKTMARVKNVYXQLKGNCERLGFYKLGGDVEDTNIPLEHKIVKCFIAGFPNHIVKLSNGGVYKKYAPTGGASLSAQSLPSLFMHPSCYFVKRQKITQQMPKPYKLVLYNQLLKTSKEFIKDCIPVFEEEWLHEMCPQMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF39</accession>
    <name>A0A1E5RF39_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Subtilase-type proteinase RRT12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1724</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RF39">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF39"/>
    <dbReference type="InParanoid" id="A0A1E5RF39"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04077">
        <property type="entry name" value="Peptidases_S8_PCSK9_ProteinaseK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000007">
        <property type="entry name" value="Subtilisin-like serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034193">
        <property type="entry name" value="PCSK9_ProteinaseK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF13">
        <property type="entry name" value="SUBTILASE-TYPE PROTEINASE RRT12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184747" evidence="7">
        <location>
            <begin position="20"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="8">
        <location>
            <begin position="222"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="262"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="418"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="9">
        <location>
            <position position="407"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="9">
        <location>
            <position position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="557" mass="61714" checksum="E376A2DB532AAC8F" modified="2017-01-18" version="1">MRKVLIKLLFIELVTFTFGSCSSEFLVRLRNPLTLKSLIAEPAGENXSDLLSRNTDTNVASFHLNTLSTLIKQKEINITKKFSFGGFEAFNAELTLNVLNKLKENXFIAEVVHNDIFQAFDHEEDLETSGDTINDGMDNENKQVYKNGTGFWSKDMENEYQEGKSDGDDASLEFFLTQYNAPRHLARCSRREKLPKNSMVELDNGKMETHSFNYYFDPLYQGQSVNVYILDTGIXSAHPDFENRSFHGADFVKEGPGDLNGHGTHVAGIIGSKSFGVAKKVNLIEVKCLDRKGEGDLSSVIGAIQFAVEDMKQTGKPSVANLSLGAFRNPTLNEAVRAAVKSGLVMVVAAGNSNTNACLNSPLSEDAAITVGAIDDRTDTLAKFSNWGECVDIFASGVLVESLSIXDSVKPVIFSGTSMATPSVTGLVAILLEKGFKPEHIKDQLLQISTKHFMKRRTFLLKPKTPNKLLFNGVEKEDDVFQSGSTSDEEENYFYDSFSANXXANISHDMVTGEDQQQDGKRAMSIMDGVKNDIHMMAKRXLWHEPSFEELQTSINF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF52</accession>
    <name>A0A1E5RF52_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative nitrilase-like protein NIT1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2012</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbon-nitrogen hydrolase superfamily. Nitrilase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF52"/>
    <dbReference type="FunCoup" id="A0A1E5RF52">
        <property type="interactions" value="744"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF52"/>
    <dbReference type="InParanoid" id="A0A1E5RF52"/>
    <dbReference type="OrthoDB" id="10250282at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000257">
        <property type="term" value="F:nitrilase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07564">
        <property type="entry name" value="nitrilases_CHs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000132">
        <property type="entry name" value="Nitrilase/CN_hydratase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044149">
        <property type="entry name" value="Nitrilases_CHs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46044:SF1">
        <property type="entry name" value="CN HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46044">
        <property type="entry name" value="NITRILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00921">
        <property type="entry name" value="NITRIL_CHT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="2">
        <location>
            <begin position="2"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="332" mass="36655" checksum="9BCF668C97EDFC95" modified="2017-01-18" version="1">MVKQVVAALQIGSSPRGTQDTLQNILKYENEIKEKQCKLVVIPEATLGGYPKGSTFGAYLGYRTQEGKEEYARYYNQAVVCDTTGAEIQKLSEFSNKTGAFLVVGCIEKVPDQHKTLYCTMVFIDPVKGFVGKHRKIMPTGTEKLIWSLGDGSTLTAVDVPLEHNKKMKIGGAICWENMVPLLRHAMYSKGLDLWVAPTVDQRPIWRSVMQTIAYEGRLFLVSAVQYMPDATTMGYGVEDPNDPQKKILPGWPESDVPCINGGSVIVNPYGEIIAGPLLGKEGLLTGEIDLDLIVEARYDFDPVGHYSRNDIFQLTVNEAPTNNGVKFVNGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF85</accession>
    <name>A0A1E5RF85_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1923</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF85"/>
    <dbReference type="InParanoid" id="A0A1E5RF85"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF236">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="339" mass="37845" checksum="189A96B0F8260B33" modified="2017-01-18" version="1">MDYSSVPPAPRASIINFVVQFYQGFRPFAPHFLPNHYPDLTGKVAVITGMNTGIGYHVTKLLLAQNCEVYGLVRTXSKGEEAKARILKELGTEEKGSLTIVGGCELSDFAKVKEVGLKLQNELLKDKTINIIIHNAGLMSSLKEPSNKKEGIELMFATNVMGPQLLQNYLDPLFLKKDSDLKRIVWVSSCAHQMAPSTYGINFKDPMYKDVSKRPASHFLYGQSKACNILQAKAWATKTNALEYGIVSVSCFPGILNTELTRDYPSFLQSVWAKIFYPPLYGAFTELYAALSPKIKEQGLYVVPFGDTRPPRADIAEALKNGVDVKFWDFVQSQLKEYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFB3</accession>
    <name>A0A1E5RFB3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">V-type proton ATPase subunit E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2225</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase E subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFB3"/>
    <dbReference type="FunCoup" id="A0A1E5RFB3">
        <property type="interactions" value="756"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFB3"/>
    <dbReference type="InParanoid" id="A0A1E5RFB3"/>
    <dbReference type="OrthoDB" id="10263003at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.30">
        <property type="entry name" value="ATP synthase, E subunit, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00311">
        <property type="entry name" value="ATP_synth_E_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038495">
        <property type="entry name" value="ATPase_E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002842">
        <property type="entry name" value="ATPase_V1_Esu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45715">
        <property type="entry name" value="ATPASE H+-TRANSPORTING V1 SUBUNIT E1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01991">
        <property type="entry name" value="vATP-synt_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160527">
        <property type="entry name" value="V-type ATPase subunit E-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="234" mass="26386" checksum="2E421BA93A2F45C7" modified="2017-01-18" version="1">MSTAAAHPLNDNQVNEELNKMQTFIKKEAEEKAKEIKLKADQEYEIEKTQIVRGETIAIDSSIASKLKKATLTQQITKSTIANKMRLKVLQAREQILENIFEESEEYLKTKVSSDKKKYGPILKDLILEGLYKLLEPEVVVKVKEEDVKLVESLIPDLVKEFTSATSLDSVNIEISKSDFLSKNIAGGVIVTDASGKISVDNTLEERLKLLREESLPAIRLELFGPSESRKFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFC6</accession>
    <name>A0A1E5RFC6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phenylalanine--tRNA ligase alpha subunit</fullName>
            <ecNumber evidence="5">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Phenylalanyl-tRNA synthetase alpha subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g2389</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFC6"/>
    <dbReference type="FunCoup" id="A0A1E5RFC6">
        <property type="interactions" value="1052"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFC6"/>
    <dbReference type="InParanoid" id="A0A1E5RFC6"/>
    <dbReference type="OrthoDB" id="238316at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00496">
        <property type="entry name" value="PheRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2330:FF:000002">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000028">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004529">
        <property type="entry name" value="Phe-tRNA-synth_IIc_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040725">
        <property type="entry name" value="PheRS_DBD3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00468">
        <property type="entry name" value="pheS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003210">
        <property type="entry name" value="PRK04172.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF40">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18553">
        <property type="entry name" value="PheRS_DBD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 18" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="17">
        <location>
            <begin position="233"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ85584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="505" mass="57364" checksum="79F5A58827876B83" modified="2017-01-18" version="1">MSTNDLQLDILSKLNTLGEIKSTAETFPDVDSQAILSALNSLKAHGKLEYTKSDTVQYTLTKEGDEIVANGSHEIRLLKLVQSFEKLQIKEIMTKMGPDGKIGQAKAFKNGWISKNKDNEITVSDKIKDKVDSINDETKDLLIKIAKNGDSAEKEIDSKVLNDLKKRKLVAPKKISIFSVVKGADFSTTLTKMETDLTSDMITSGSYKDLKFRSYNFNSQGAMPASGSLHPLNKVREEFRQIFFSMGFSEMPTNQYVETGFWNFDTLYVPQQHPARDLQDTFYLKDPATCDLPSDKQYIENIKAVHEQGKFDSIGYRYNWKEEESKKLVLRTHTTSVSAAMLHKLAQDPKPTRLFSIDRVFRNEAVDATHLAEFHQIEGVLADYDITLGSLIKFMEDFFAKMGVTGLRFKPTYNPYTEPSMEIFSYHEGLGKWVEIGNSGMFRPEMLESMGLPRELKVLGWGLSLERPTMIKYKVQNIRELLGHKVSLDFIENNAAARLDEDLYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFC9</accession>
    <name>A0A1E5RFC9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative mitochondrial outer membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2274</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFC9">
        <property type="interactions" value="81"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RFC9"/>
    <dbReference type="OrthoDB" id="1641132at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025423">
        <property type="entry name" value="TMEM205-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053009">
        <property type="entry name" value="Xanthocillin_Biosynth-Assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23241">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT PLANTS LEA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23241:SF102">
        <property type="entry name" value="LD23009P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13664">
        <property type="entry name" value="DUF4149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="TMEM205-like" evidence="6">
        <location>
            <begin position="12"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="167" mass="18943" checksum="82BBF314ECEE158D" modified="2017-01-18" version="1">MSFIKPAAHLFTFSFLFGGNIFYSYIASPIAFKHLDRENFSKLQNKVFPIFFQIQSLAPALLFLTLPSKSILYTKAPLISLAVCAASGLANLVWLLPWTRKVKEQRKHLAQKYSAXDQKHLLEIEDAPLRKEFGKSHGLSLLFNLTHCVSLTVYGAYLVKLLKFVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFE5</accession>
    <name>A0A1E5RFE5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vacuolar protein sorting-associated protein 27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g1744</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFE5">
        <property type="interactions" value="119"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFE5"/>
    <dbReference type="InParanoid" id="A0A1E5RFE5"/>
    <dbReference type="OrthoDB" id="957735at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033565">
        <property type="term" value="C:ESCRT-0 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049425">
        <property type="entry name" value="Vps27_GAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47794">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47794:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02809">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21356">
        <property type="entry name" value="Vps27_GAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="VHS" evidence="13">
        <location>
            <begin position="20"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="12">
        <location>
            <begin position="183"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="250"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="297"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="547"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="313"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="327"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="580"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="626"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="14">
        <location>
            <position position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ85604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="641" mass="73052" checksum="A122BD3B59BCCEAC" modified="2017-01-18" version="1">MSLPSVSVSKFENLIEQATSETIPNGEIDLIRSLEVSDIIKSKSLPTQQCLKVLIKEVNAANSPPIDLNSQLSIWRLIEICFKNGGESFIKELLSNEFMKILETSIVKAYEKNYETVELFDLLSQVLYDMKNYCEDLNKINYPYNNPAKVWNRLQDYEGITFSYNKLVKNDIKTFDSKQPADWVDADSCMICSANFSMFNRKHHCRSCGGTFCQKHSSNFIPLLDLGILEAVRVCDNCFEDYDLKKNKYSKKADKHHSPKSKTSKHRVKGQKVGHSSKGDSFDEELRRAIELSLQEQSLXVEGNEPAPQEPVAKVRESGSYHRGTNEEQEEEEKEEEEEEEEDEDLKRAIELSLKEAEKSGAKLQDQTASLAQSQSFVNMQTTALNSDQAPPMPSNPNEITTKDEDDIYLFASLVEKLKTQPLHIVLQDSKLSQLYANITHLKRKINLELQETDNKYGTFIQMNSQISDIMNVYDQILESQLNSINLQQNQAIMQPSDPDSYYKQFAQPAQPVPQQQVHETKQTYAEQHNEPPVANQLENLVFPQSELQESEPPMTEESEPPAMTEXKPQVKSTPYPTDHFSDKVSEEKITESAPYPITQVNFPTVPASKPLDAELKNQESQETAKVAEEEHHEEPMLIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFG5</accession>
    <name>A0A1E5RFG5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">pH-response transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2313</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the pacC/RIM101 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFG5"/>
    <dbReference type="InParanoid" id="A0A1E5RFG5"/>
    <dbReference type="OrthoDB" id="6155966at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050806">
        <property type="entry name" value="pacC/RIM101"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47257">
        <property type="entry name" value="PH-RESPONSE TRANSCRIPTION FACTOR PACC/RIM101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47257:SF1">
        <property type="entry name" value="PH-RESPONSE TRANSCRIPTION FACTOR PACC_RIM101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="154"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="330"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="10"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="44"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="344"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ85624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="751" mass="80449" checksum="3ED3F8C3B3C4A7B9" modified="2017-01-18" version="1">MSSVHALLNTAHSATNVGSDAAKGNTSSKXQQQTHANEATTPLSAPMSESSSPKSASFTLPPAXAATSGSPASYASPSLTAAGSPSSSPSSSSSPSTQPNKSNIVDRNNEDRTDKPIMCQWGSSCNLLFPDAETLYKHLCQDHVGRKSHKNLQLQCQWGNCKVATTKRDHITSHLRVHVPSKPFSCSNCHKKFKRSQDLKKHLKVHFKDILGEGGISKKTKMGSQHTNNMIHGNLNNIMNNNMNNHKHHICNHHLCNHHQHHNSTLVTPSVNSMFLGNTLHRQDRNVVILPPLGNPMAAANNRVGGNQMPLNXNMNMPLNTNMXLNTNTNMNMGLSGYKSTARSGSSEGNSSQSGSSPLNSTYKSTITNNINSTNQNAAPTTATTTNSPPSISNLSPSLPALKKVSHFFNSLANDAVLGAXNNTLLGYPSLPPAHTNIXNNNTISSTIMNKSGASSSTSSPPAQLHQSQLAVPTTIANHQQHTAQLPLPGLGSVGSQSGSLSHANSTTPTLLAQPGSSYPILPVNNYAPLPPLRTPQTASFATTGYWNNGYAPSLPFENSAIPSLNHNKAAAXQQYSYYSAYGANNAMKSTDAILLAHQQNNASSYGIFQRSTAPDESLSGSPELSESMAALXISSGSTNGKEIDTTKVTVDEIRDVLLYVVQELGLSSDDDTEEENKHEGQAXASYFSEAPLELDVETFQAIKDYVMCTYRELSGKHELDGEDESDFVDAGPXTLQXNTFIEMKYPTISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFS0</accession>
    <name>A0A1E5RFS0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 17">Pyruvate kinase</fullName>
            <ecNumber evidence="6 17">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AWRI3579_g2204</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the pyruvate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="6 17"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFS0"/>
    <dbReference type="FunCoup" id="A0A1E5RFS0">
        <property type="interactions" value="967"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFS0"/>
    <dbReference type="InParanoid" id="A0A1E5RFS0"/>
    <dbReference type="OrthoDB" id="108365at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00288">
        <property type="entry name" value="Pyruvate_Kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.33.10:FF:000001">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000025">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1380.20:FF:000001">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="entry name" value="Pyruvate kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018209">
        <property type="entry name" value="Pyrv_Knase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004491">
        <property type="entry name" value="PRK05826.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004978">
        <property type="entry name" value="PRK06354.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="PK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00110">
        <property type="entry name" value="PYRUVATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14 17" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 17 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0630">Potassium</keyword>
    <keyword evidence="15 20" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="18">
        <location>
            <begin position="23"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate kinase C-terminal" evidence="19">
        <location>
            <begin position="384"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OEJ85724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="504" mass="54818" checksum="62A6738DE04CA25F" modified="2017-01-18" version="1">MESKLEWLTKLNPTASATAPALRRSSIIGTIGPKTNNPEVLVELRKAGLNIVRMNFSHGSYEYHQSVIDNARKSEELFPGRPLAIALDTKGPEIRTGTTTNEVDYPIPPNHEMIFSTDAKYAKACDDKVMFIDYANITKVITKGRIIYVDDGVLSFEVLEVVDDKTLKVKSMNAGKICSHKGVNLPGTDVDLPALSDKDKADLRFGVKNGVHMVFASFIRTANDVKTIREVLGEDGKDIKIIVKIENQQGVNNFDEILKVTDGVMVARGDLGIEIPAPHVFAVQKQLIAKSNLAGKPVVCATQMLESMTFNPRPTRAEVSDVGNAILDGADCVMLSGETAKGNYPVNAVKMMAETALIAEQAIAYGPVYDDLRNTTPKPLSTTETVAAAAVAAVYEQEAKAIIVLSTSGNTSRLVSKYRPNVPIILVTRNERAARFSHLNRGVYPFVYSEAVASEWTVDTEKRFQFGIQKAKEFGFLSEGDAVVTVQGSSAGIGHSNTLRIIQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RG40</accession>
    <name>A0A1E5RG40_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2286</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RG40"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="unsure residue" description="D or N" evidence="1">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="1">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ85543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="186" mass="21353" checksum="EC91B7E656966DF9" modified="2017-01-18" version="1">MNSIQPMQXKNMSFKAQETNPTLLSDIHQIDSLNALFDFKHYTDRVSLENFEQXMPEDTNTTPFNAAANSIFDSYNKNYSLYSSSQQSNESIESLQIKHQLANINKQSIIQSMKPRSQDWRIPNMKPKAQDYHITKKSAKNSKKLIQNQQKVTQTNINTQFSNCDIKDQATFILLNDLITGDAFDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGF8</accession>
    <name>A0A1E5RGF8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1482</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ85991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGF8"/>
    <dbReference type="InParanoid" id="A0A1E5RGF8"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051647">
        <property type="entry name" value="Mediator_comp_sub12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007:SF12">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="177"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="292"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="344"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="385"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="424"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="481"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="129"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="50"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="83"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="187"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="292"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="396"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="429"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="513"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="3">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="556" mass="60133" checksum="9C19D848F41DD3F4" modified="2017-01-18" version="1">MEETNSGTGVSNDEKKNVNDSRSSSASPHTVKKERDPLLEIIDASPSFATLNNDKTKGXGTTSNTSTTTPSSLGSGTPVSDXGPNSNPTASSHPITIKPKRQYRKSNNVASGPTSNTXSTNVGPKKTKKQLKLEEMLALEKELESLTQENGKLKSVMFKIKQEIELYSQYMSKYSDYTDPSFVMPTLPSTSAKGKNKKSTAANKRTSKKSISDVSSSGATGDLGTPMVKQPASVVQDIPLSDYIQQQKKQQQQQQQQQQQQQQQQKQQQREDEDGLHTMTSLKDILINTSQIGSNTNSVSDGSITPISTKKRRIKGKNPAVMNEVSGLSSRQFLENVFSMDNGLSTSTSSASTPPPPPPPLSSFSLTSALSEVITSDDLPSLGGNLDFLSTDDAGNPTTTNNTSPNNAQIKKEYNQRNKLEFENENGKENSSSNNNNNDDLIVLPVMPNSPSSFFSKNGLLDSVILETGFSPMSFTETTTTSSMIASKPASRDMHSVPAGSNTSKQHPKDNKSNASQDNLQKPLNLNTFPGQPTTGNLEADDLMLMNTLKDVLSHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGH0</accession>
    <name>A0A1E5RGH0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1086</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RGH0"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ86010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="320" mass="36572" checksum="A686169EE9120713" modified="2017-01-18" version="1">MGLYRNLXQDPGFPLLTQWKQILKNCKXEQXATDTXHSSIECDRKVVTLDELXRLFKIKLFKQKKNRDQRQIEXKDIDNIHNKKSKLDTVRVSKSEFESIKSEMMMHSGKQLPKVPQVGTFANVSQKTNNLESTYDTETVEGHLSATXNTYLNDTEPFSEMSVXGKNDGVVXHKLSSSNFEETLNQSNQQLLLSSSLYTTNTFVNSNIDFLHKESEKNEQDTKKLEQFGENAMLLSSTGVNTSYYITSDLKKMDGQXXXQYIANNPYKKTKIDYENNFCQLKDSSPPFHQAKELQELNVDPLCSSPCSSSKSFSSLIQFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGN2</accession>
    <name>A0A1E5RGN2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquinone biosynthesis monooxygenase COQ6, mitochondrial</fullName>
            <ecNumber evidence="11">1.14.15.45</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">2-methoxy-6-polyprenolphenol 4-hydroxylase</fullName>
            <ecNumber evidence="11">1.14.15.46</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">COQ6</name>
        <name evidence="13" type="ORF">AWRI3579_g1562</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">FAD-dependent monooxygenase required for two non-consecutive steps during ubiquinone biosynthesis. Required for the C5-ring hydroxylation during ubiquinone biosynthesis by catalyzing the hydroxylation of 4-hydroxy-3-(all-trans-polyprenyl)benzoic acid to 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoic acid. Also acts downstream of coq4, for the C1-hydroxylation during ubiquinone biosynthesis by catalyzing the hydroxylation of 2-methoxy-6-(all-trans-polyprenyl)phenol to 2-methoxy-6-(all-trans-polyprenyl)benzene-1,4-diol. The electrons required for the hydroxylation reaction are funneled indirectly to coq6 from NADPH via a ferredoxin/ferredoxin reductase system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 2-methoxy-6-(all-trans-polyprenyl)phenol + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) = a 2-methoxy-6-(all-trans-polyprenyl)benzene-1,4-diol + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O</text>
            <dbReference type="Rhea" id="RHEA:81183"/>
            <dbReference type="Rhea" id="RHEA-COMP:9551"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10858"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:62731"/>
            <dbReference type="ChEBI" id="CHEBI:84166"/>
            <dbReference type="EC" id="1.14.15.46"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 4-hydroxy-3-(all-trans-polyprenyl)benzoate + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) = a 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoate + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O</text>
            <dbReference type="Rhea" id="RHEA:81195"/>
            <dbReference type="Rhea" id="RHEA-COMP:9514"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10930"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:64694"/>
            <dbReference type="ChEBI" id="CHEBI:78396"/>
            <dbReference type="EC" id="1.14.15.45"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion inner membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the UbiH/COQ6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.15.45" evidence="11"/>
    <dbReference type="EC" id="1.14.15.46" evidence="11"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGN2"/>
    <dbReference type="FunCoup" id="A0A1E5RGN2">
        <property type="interactions" value="402"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGN2"/>
    <dbReference type="InParanoid" id="A0A1E5RGN2"/>
    <dbReference type="OrthoDB" id="683240at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120538">
        <property type="term" value="F:2-methoxy-6-polyprenolphenol 4-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106364">
        <property type="term" value="F:4-hydroxy-3-all-trans-polyprenylbenzoate oxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000021">
        <property type="entry name" value="Ubiquinone biosynthesis monooxygenase COQ6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03193">
        <property type="entry name" value="COQ6_monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018168">
        <property type="entry name" value="Ubi_Hdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010971">
        <property type="entry name" value="UbiH/COQ6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051205">
        <property type="entry name" value="UbiH/COQ6_monooxygenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000689">
        <property type="entry name" value="UbQ_mOase_COQ6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01988">
        <property type="entry name" value="Ubi-OHases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43876">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS MONOOXYGENASE COQ6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43876:SF7">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS MONOOXYGENASE COQ6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01304">
        <property type="entry name" value="UBIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0274">FAD</keyword>
    <keyword evidence="3 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8 11 13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0830">Ubiquinone</keyword>
    <keyword evidence="4 11" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="FAD-binding" evidence="12">
        <location>
            <begin position="388"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ86069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="520" mass="58381" checksum="2C41CCD47ECA6924" modified="2017-01-18" version="1">MFSRFSTALSFRQASLRVAKQHRKLYSTEVKQVQDKILTDILIVGGGPAGLTLASAIKSSPLLKHYKTTLVDATPISSSLPTFYEKASSDEQFPFTNRVVSITPRNMDFLTADLNLPLMFNRIQPFDGICISDGLSDFNTLTMDRDSMGYMVELLNLQSSAHQKILELNEKFIDPNEKIQIIDNCKVLDIKSPTVEDNAETNLCSDAWPEVELDNGQIFKTRLLIGCDGFNSPVRKFANIDSLGWFYNKWGVVATMKLECPPFSQIRGWQRFLPTGPIAHLPLPNDNATLVWSTTPELSQLLTKVPAPVFAKLVNAAFLLEDGDMQYYYNKLRQVNDLSNENDPIYSEMIEDINYRMEDKSEHITKFYGKNDMYFLEENYPPTVVDIQDKSRARFPMKMFHCNTYVAPRVALVGDAAHATHPLAGQGLNMGLGDSMSLVKALEQASSRGLDLGQTDFVLNEYWKERYGTNMMLLGITDKLHKLYGSSNPIVTTARQFGVSVLNNIGGLKSLMMNIVSGPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGZ4</accession>
    <name>A0A1E5RGZ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1307</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RGZ4"/>
    <dbReference type="OrthoDB" id="5319015at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="460"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="539" mass="62589" checksum="DAA5183317DFB5B4" modified="2017-01-18" version="1">MLKPLKNSKGCWGVGQFISNRQRWTTKQWKFKETTPYSTTTGSATPNSLDHLREIKSNQWLKLSQLIKLEQNPPKLNTLLSYLSNSASGTPVSSNKKRIGVLSSSEKPSYTIQCLLMDPYHSDQEWVGIFNNRYKLHKEQQHQMQDIDEMRNGQAEEIVVNVLKYSPFLEINTVKNANGVSTMTYGVPCKKLLQDNIEIVEYKSRHYGVMQKIDQVLFYLNTDPNFHEIKYPHINVKEGKRALKNGNQTDTFSIDSAEMLQFLQVFYKDKTSVNKYLKEWDDCGFTVLEKELAKXFANYSEFVVKPVERXVLDEIILKDKDLNSFLKSNTSLQQPSNSDNSIYLDKINQWYTDSHRHLQAEIMAIKPCTLKEIYLYSESKLELTLLRKLNTIKPIIDNLNYIKGQLSIPQKTTVQEFQIIDVQSVVKDLHHQINSLVYSNFWKLQLPMVLXGTLSVLTNLYTLQTMLPIVLFGVMLGVFRVSMGVKRLWTKVVNRKVIDPVRNFIESENKFLVEQSNLSNNHLQTMKREKLNLIKKLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGZ7</accession>
    <name>A0A1E5RGZ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Glucose-6-phosphate 1-epimerase</fullName>
            <ecNumber evidence="3 5">5.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1436</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the interconversion between the alpha and beta anomers from at least three hexose 6-phosphate sugars (Glc6P, Gal6P, and Man6P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 6-phosphate = beta-D-glucose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:16249"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="ChEBI" id="CHEBI:58247"/>
            <dbReference type="EC" id="5.1.3.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the glucose-6-phosphate 1-epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.15" evidence="3 5"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGZ7"/>
    <dbReference type="FunCoup" id="A0A1E5RGZ7">
        <property type="interactions" value="277"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGZ7"/>
    <dbReference type="InParanoid" id="A0A1E5RGZ7"/>
    <dbReference type="OrthoDB" id="1659429at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047938">
        <property type="term" value="F:glucose-6-phosphate 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09020">
        <property type="entry name" value="D-hex-6-P-epi_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025532">
        <property type="entry name" value="G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11122">
        <property type="entry name" value="APOSPORY-ASSOCIATED PROTEIN C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11122:SF13">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016020">
        <property type="entry name" value="PHexose_mutarotase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="6">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR016020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR016020-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR016020-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="300" mass="34063" checksum="B650D98E71D17167" modified="2017-01-18" version="1">MPITETDTTVVLTHPENQKSTVTILKYGATVYSWKLDGKEQLWLSSAAKLDGSKPVRGGIPLVFPVFGKCTDSVNHPELSQLPQHGLARNSTWEFLGQTTESPLTVQFGLYPEIANSELTKIWNYDFELILSVELGVDYLKTAIEVTNPLKATRELSFNWLFHTYLHVEDVEDTMVANLAGSNTYDQLLQESYTEVQPVIGFHEELDRIYRNVNPERVIQVVDKGHPLHSVKRSNLPDAVVWNPWIEKSKGMGDFEPKDGYLKMVCVEPGHVHDFVTLKPGAKWEAFQLLYKDELNYQAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH43</accession>
    <name>A0A1E5RH43_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Checkpoint serine/threonine-protein kinase BUB1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1126</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
            <location evidence="1">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH43">
        <property type="interactions" value="530"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH43"/>
    <dbReference type="InParanoid" id="A0A1E5RH43"/>
    <dbReference type="OrthoDB" id="248495at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051754">
        <property type="term" value="P:meiotic sister chromatid cohesion, centromeric"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13981">
        <property type="entry name" value="STKc_Bub1_BubR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015661">
        <property type="entry name" value="Bub1/Mad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013212">
        <property type="entry name" value="Mad3/Bub1_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012572">
        <property type="entry name" value="Mad3/Bub1_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14030:SF4">
        <property type="entry name" value="BUB1 KINASE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14030">
        <property type="entry name" value="MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08311">
        <property type="entry name" value="Mad3_BUB1_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08171">
        <property type="entry name" value="Mad3_BUB1_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00777">
        <property type="entry name" value="Mad3_BUB1_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51489">
        <property type="entry name" value="BUB1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0137">Centromere</keyword>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BUB1 N-terminal" evidence="7">
        <location>
            <begin position="51"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="654"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="558"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="558"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="962" mass="111449" checksum="AB4AEB2B01B353E5" modified="2017-01-18" version="1">MSNHRHSASNFSDLESQKENISRIRSGRSAAQLVETFQXQDQDLNRXRLKFEKQILEEIEDADDPLELFLNYINWIKIKYPQGITTKRSGMLQLLERCLQXFKADALYINDPRYVKLWIWYIEVFQTTLHDKKDTLYYMVRHDIGAKLALVYESLANVLWDLRAYEEAVSILRQGLVAECRPLKRLQKQLDLFTKQHDKIKVETVPVKSVSDLNRNILGQSKQSILRDYERDEQSAGTPPNRQKKAKLDIYIEXEDENYNLHAPKTGVANHELREFPSKYKLSKENQKLAEPVQNGIKLKPLNSIEEQRISKLENALLLSKPKTAIFSDTNGKLEPKYKVMHIPNKNPERIDVNYDLLYPNPGQELCLDEILAIKRNVYKKLDKLSGKARILKEKPIXPFPSEVYRDETIQLRSPLKPEPRPTGIVSNAVVLQPASPTITMHSKNAMNEIYNMFNHNEFGHVPEALSSRLPEDENTERRFEMFENFTQDFTKKSLDDLTEVRPFGKSPVKTPRSVSKNQKLKSFMTPLKDSKVLENFSSPQRKPTIDVTSLKISNLSTNPSYLQENPSSSHNFQHSXEKPIPSNPSLVVKDPLNDSYRQHLLSSTKPLNDYIGFHKYEYDLKMSGQLSRINKSSNKKNMNKNPIINFQKTDDLYFIRGELGQGGYATVYLAESSNGALKALKVEKPPNLWEFYVLRQISERLEDEKRILQSIILVDSLHFFRDESYLVLNYISQGTILDLINKERNKSSTGQSFNELLVIFFSIELMKVIESLHQINIIHGDLKPDNCMIRFEPFDSDIGDYNSSGLNNWDKKGIFLIDFGRSFDMSILPPGTKFKANWKCDDQDCPEMRENKEWTFEADYYGLAGIIHCMLFGTYIKTIKVKGRYRLLNGLKRYWQKALWEELFDLLLNSAEYAETLNNGSLPITSLLTAQRLKLEXYLMQNSNSEKLKNIIHEIEMDING</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHK7</accession>
    <name>A0A1E5RHK7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mitochondrial inner membrane i-AAA protease supercomplex subunit MGR3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1471</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHK7">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHK7"/>
    <dbReference type="InParanoid" id="A0A1E5RHK7"/>
    <dbReference type="OrthoDB" id="10050400at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031942">
        <property type="term" value="C:i-AAA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24145">
        <property type="entry name" value="Mgr3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040201">
        <property type="entry name" value="Mrg3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28142">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE I-AAA PROTEASE SUPERCOMPLEX SUBUNIT MGR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28142:SF1">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE I-AAA PROTEASE SUPERCOMPLEX SUBUNIT MGR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="525" mass="61014" checksum="FC987ECE597AECE4" modified="2017-01-18" version="1">MFITNTLLRSRALASASSNLKRPVFTFQRTLWLFNKKKAAESPQVINPTLLQQHQXQQRDDAKTQRKTLIFRSVLAITIGLSAFVLYAWPRHTFPSSVAKELRKGLWEEREKNKDNHNRPNHQKALEYYISALNRAKEEELDTLSDEYTGIELKIAEMYEKLNMIEAXSKLYLELSYRYYTALNDPENPHGIKTMERGHYIQKDLRVLIKFVQNQMNSKNVDLAVLNFNKRLLTSHIIMAEKEVFMKSTELPEKYMTQFRNDMLKKKSRSNEFAAKLNIKGYNFQPFENVVDENNIHFNKDGYMELDLNQKSSYAWDXFKSEFFVARDLYSAICLSTKDVPSAIYNKMNTLNLMVLAGMEPGQILMTQANLGSLLYLRVEQMEDEMDFMERNPEKFKELLANKTENTDDQLIQVQRLMNRARDVCFKSATECYESIIKFSSKHKRLKFNYKDLADTNVGQAIALSKYGLGVLRFHEGNLQEAKILLNDGKQMASEIAFTDLVNEADNELAKLNKAMTAEQQKQSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHM6</accession>
    <name>A0A1E5RHM6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 15">Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase</fullName>
            <ecNumber evidence="3 15">2.7.4.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g1249</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Bifunctional inositol kinase that acts in concert with the IP6K kinases to synthesize the diphosphate group-containing inositol pyrophosphates diphosphoinositol pentakisphosphate, PP-InsP5, and bis-diphosphoinositol tetrakisphosphate, (PP)2-InsP4. PP-InsP5 and (PP)2-InsP4, also respectively called InsP7 and InsP8, may regulate a variety of cellular processes, including apoptosis, vesicle trafficking, cytoskeletal dynamics, and exocytosis. Phosphorylates inositol hexakisphosphate (InsP6).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1D-myo-inositol hexakisphosphate + ATP = 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:37459"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:74946"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:37460"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate + ATP + H(+) = 1,5-bis(diphospho)-1D-myo-inositol 2,3,4,6-tetrakisphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:10276"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58628"/>
            <dbReference type="ChEBI" id="CHEBI:77983"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:10277"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cytoplasm</location>
            <location evidence="1 15">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the histidine acid phosphatase family. VIP1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.24" evidence="3 15"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHM6">
        <property type="interactions" value="554"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHM6"/>
    <dbReference type="InParanoid" id="A0A1E5RHM6"/>
    <dbReference type="OrthoDB" id="18042at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033857">
        <property type="term" value="F:5-diphosphoinositol pentakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052723">
        <property type="term" value="F:inositol hexakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052843">
        <property type="term" value="F:inositol-1-diphosphate-2,3,4,5,6-pentakisphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006020">
        <property type="term" value="P:inositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000036">
        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11950:FF:000002">
        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037446">
        <property type="entry name" value="His_Pase_VIP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040557">
        <property type="entry name" value="VIP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12750">
        <property type="entry name" value="DIPHOSPHOINOSITOL PENTAKISPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12750:SF9">
        <property type="entry name" value="INOSITOL HEXAKISPHOSPHATE AND DIPHOSPHOINOSITOL-PENTAKISPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18086">
        <property type="entry name" value="PPIP5K2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 15 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-grasp" evidence="17">
        <location>
            <begin position="453"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="65"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="143"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="251"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1021"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1272"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="13"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="67"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="251"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="306"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1031"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1272"/>
            <end position="1299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU365032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ86400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1314" mass="147142" checksum="C9FF20ABCA1F2851" modified="2017-01-18" version="1">MHEIPKDLDLSRESSSTPVQLNRDGTLTYNENEQTCPGSPMTPQELQRNTKKAMESIAPILEGFVPKTSSSENTSLKISTPSVPVAPLNSSLSTPHEEDELVSLPDYHDAAALESLATLEKTLSHTSKPALSPMNSMLDFHSHSKPNTEDLLNISKDMKPPGVLSPGASASTSRRSSVSSYVNARSSSNPISFLGLGNSSKSFNSANNGGSNNNAHHVFAPTGSTNSFFSGRPANEHSTLSQAINESKITAPSLDSLSGTNAATTDPATAKIPSNSELRSVLSNSSSVSNSNLSLSPMDDQIGENSTTPSESSKAPSESPAASVKTKIGKIGVCAMDAKVLSKPCRRILNRLMEHGEFETIIFGDKVIFDENVENWPTCDFLISFFSNGFPLDKAIKYAKLRKPFLINDLILQKVLWDRRLCSIILEQAGVSAPPRLEISRDGGPMVTNADLKEKLAELGIDASKKVKEPEWRMVDQDTLEVAGKQMVKPFVEKPVDGEDHNIYIYYHSKNGGGGRRLFRKVGNKSSQFEPELSDIRTDGSYIYEKFMDADNCEDVKAYTVGPTFCHAETRKSPVVDGVVRRNTHGKEIRYITELSDEERDMAAKICTSFQQTICGFDLLRAHGKSYVIDVNGFSFVKDNNNYYDSSARILRQMFIEAKKKMDLEKKSMPVIQEEKQQKWVFKGLITVIRHADRTPKQKFKFSFTSPIFISLLKGHKEEVIIRNITDLEIVLKALKIAREDFLEDVNKLDILINALSKKIEFPGTKIQLKPVINKESKEVEKVQFILKWGGEPTHSAKYQASELGEQMRQDFDLLNKSILDNITIFSSSERRVLYTAQLWASALFGEKHGKEIQIRKDLLDDSNAAKELMDKVKKKLKPLLRKGEEAPSQFAWPANMPEPYFVIKRVCELMNYHKIIMEHNFKTKDVDAMQKVWCCAEDSMLFRERWDKLFKEFVNVEKVDPSKISELYDTMKYDALHNREFLEKIFAPDEEKMNDDLVHSSLVDRYPINLLAKNNFKITSENSGESKPRSVSTSSTATATPNKSTVGSLGWVLESGQEPVSESSSHFDGPQFIQLRELYRLSKVLFDFICPQEYGIKDTEKLDIGLLTSLPLAKQIFKDIDEMKVKEEPQCVAYFTKESHIYTLLNILYESGIPMRIARNALPELDYLSQINVELYESVDKTSKKKSHAIRLKMSPGCHTQDPLDVELDEKHCISCIPKLSLTKHLDADYVEQKLRRKFPRVTMPKKFTPVNITNPDLSFKRAVSKKEKVSATVVAPSNSSSSSSVINESTSSVTISSLKTSADFPDPAETKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHQ8</accession>
    <name>A0A1E5RHQ8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1658</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHQ8"/>
    <dbReference type="InParanoid" id="A0A1E5RHQ8"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="118" mass="13232" checksum="0647F9F1D9CF412E" modified="2017-01-18" version="1">MTIKNKYAKPSGSKKNNANTKGNIKKHNTPLSKNTKKRTKHQLQQLNKEDIDLASVHQEIKAGASTACSVLPQQKLTGKQLLELNKQDVATQKEKKEKDEKVANDLVKQLELIDGFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI50</accession>
    <name>A0A1E5RI50_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">RNA polymerase II transcriptional coactivator SUB1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1359</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transcriptional coactivator PC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI50"/>
    <dbReference type="FunCoup" id="A0A1E5RI50">
        <property type="interactions" value="57"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI50"/>
    <dbReference type="InParanoid" id="A0A1E5RI50"/>
    <dbReference type="OrthoDB" id="3972567at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.31.10">
        <property type="entry name" value="Transcriptional Coactivator Pc4, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003173">
        <property type="entry name" value="PC4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009044">
        <property type="entry name" value="ssDNA-bd_transcriptional_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045125">
        <property type="entry name" value="Sub1/Tcp4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13215">
        <property type="entry name" value="RNA POLYMERASE II TRANSCRIPTIONAL COACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02229">
        <property type="entry name" value="PC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54447">
        <property type="entry name" value="ssDNA-binding transcriptional regulator domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcriptional coactivator p15 (PC4) C-terminal" evidence="8">
        <location>
            <begin position="77"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="146"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="38"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="172"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="247"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="282" mass="31037" checksum="09FEBB42E17B82F9" modified="2017-01-18" version="1">MSGFNKYRRKWNKDASKGDTTSISLDQGKEGGKKGKYTSKSNNNNNNSNNSNNSNNYNNNNYNDGGNSGANVPESVFELGKSKRVTVRQFRGVNLIDIREYYKDSSSGEYKPGKKGISLTEELYDSLIRQRRNIDDALRRMGSKRSKNGLLDSIRDQDTASSTEKKSDQNDKNNTASATGSATGSEKSELKRKHEQSEGTAASGAESDKKPKLEIAPPQLLPNEAAKRQEELENTAAIVIPGGNKPQEQKKDAQAIKKEQEEGLNSSDEEFAAGLEADMQEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI57</accession>
    <name>A0A1E5RI57_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Mitosis inhibitor protein kinase SWE1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1518</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI57"/>
    <dbReference type="FunCoup" id="A0A1E5RI57">
        <property type="interactions" value="371"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI57"/>
    <dbReference type="InParanoid" id="A0A1E5RI57"/>
    <dbReference type="OrthoDB" id="5337378at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110031">
        <property type="term" value="P:negative regulation of G2/MI transition of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF196">
        <property type="entry name" value="MITOSIS INHIBITOR PROTEIN KINASE SWE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="691"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="177"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="287"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="177"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="207"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="306"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="318"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1085" mass="123281" checksum="7FCECB9551DC3FE6" modified="2017-01-18" version="1">MSATNIFVTSPTADAKKRKGSAASGCQTNTFSNTSTSNNNLTFTPHQSMDNRIEAQELFLDDDEDYMLDDEASTPFYMVPQEFGSAVQNRARTNSGSSKQGAFGLAFLISSSNTTRSKRMFCDNLFDSNSVGITRSVGHLNLSLSSRPPSPDILVTSNDIEQKHHSINEKIATWSPFNDYTQNTSNSTEQDQRHHHHHHQHDKQIPFEEEEEEEEEEEEEEYEVEEEEEDKDFSVKRKALKLSNFMAEEKLKSQKEISDVLKKWNSEKPKHKTLKNRNGLSLTLGKDCDSEYQHPGPIKQSQNGHRIKDYSSLDEGRNPNTYSEYFSKINSSNPGSSVHQQHAAKIRKKSEQDPGNKLSFINAKPDQSAFASQGLVSKLKKTNAHNEKNLNWVSNENSSVFPNTPMKKLPFLNKATWQKQNVSHQDFGETPFNKRLDSKTGHPKHSTFTIQENTITDPCQSGNIFSGYDNFHDDDMNLFATSPMKMQQQQQQQQHRVKSSSRVVMRTPFNSSNSGTKKMNRQSTVLNNNELSNTLQKFKDDLYGTTESATESLFNNSSPIQTPTKRINKLRNTPQQQQANQTGLLPPLPNTTSKEWVHHHSYTNNYDNAPQQTGKTIFKSQYDNHQPDYNVRAGHQTSLMHDSKLDYHEIGFDSPLRLNSQRDASGNNFVGLGSLYNNESKSEASYLLTKFSHVELMGQGAFSTVYKVKDGQNRNFFAVKCIVPKSTMKHIVLNEISVLQFIQEQKQKAIITKTGENAKWISSFDGGDYEEVKREHVGSRENVLQQNLVNERQIVEGKEYVFDFITSWKHSSNFFIVSEYYENGTLDDFIQTNVIDKKTRLEDWRVWKIIVEIAQGLRFLHEVCKIVHLDIKPCNVFVTFEGCLKIGDFGMSTKLPLESKAFENEGDREYIAPEIISEGIYDFRADIFSLGLMIVEIACNVCLPDNGTAWHKLRSGDLSDAGKLSSTDIQSEFSNTTTNTTSAIFSTKLSKITQLSSASNYLGQSKLQELESQTGTKVPAWVPKFLIDGQSLNKIVKWMIEPDYRLRPSADDLLKTEECEYVEMTKQSGATIHEDHFGPKPDFFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI62</accession>
    <name>A0A1E5RI62_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1351</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Na+/solute_symporter_sf"/>
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        <property type="entry name" value="Put_Na-Bile_cotransptr"/>
    </dbReference>
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        <property type="entry name" value="SODIUM_BILE ACID COTRANSPORTER 7"/>
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    </dbReference>
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        <property type="entry name" value="SOLUTE CARRIER FAMILY 10 MEMBER 7"/>
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    </dbReference>
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    </feature>
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        </location>
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        </location>
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            <begin position="469"/>
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        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="OEJ86577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="491" mass="55360" checksum="415CE48ECD24C7D3" modified="2017-01-18" version="1">MPEKHISTSSTAQPQNLNVKKSTEQLHNIAEPVEQNEKPASETPLQPRWKRYAKAVYLYCESQWFFFALAILIIIARWWPEFARDEGIISAQYSIGYLAVALIFFKSGCTIPHQKFLKNIKNWRAHFLXQVXSFLVTSAISFGFCLAILKSNNQTIDKWVLVGIIVTMNSPTTVASNVVMTKKAGGNETLTVCEVIIGNVAGAFITPALGQLYLQGSFKFGNPANGDSVSHVYRRVMQQIGCCVFVPIFVGQLLRLRFVKQINWVIKTFHLGKLGSWCLLAIMFNSFSTAFYQHAFTSVSHVTIIFVCFWNVGIYLFFTVLSFILVRPFFIPLIFKEDNNGRIATFFKKFYFNKKDATSILFCTSAKTAALGVALITSQYGNDXSKLGKLLVPLVLYQSEQVITANFMVPGFKKWIGDEGKEVTPDEATAVQAHQNDEEHHLESHNQEMDEHSQKEIFTKEEYTADEFQSGSSSELQDSNSLSNKKATNQX</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI71</accession>
    <name>A0A1E5RI71_9ASCO</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1194</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
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        <location>
            <begin position="318"/>
            <end position="339"/>
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    </feature>
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        <location>
            <begin position="351"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="401"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="436" mass="49606" checksum="2A49AE5020A2A3F5" modified="2017-01-18" version="1">MNMNSDGIAIHKDRRFVLKSVAKSWNKNIFKWISITVIMLSFIQIFMLGTLFTCTSDLEYNGNKYPETSKSVSKFQCFPLFTMSIYNSAKSHISKNTKSQNSEDVHIQHDVLGHFQKIANFLILFANDFNYNTAETTTIQQTSNAKSSLFESSFTNEYITTTNETTSNHDEIQYDISFCGYCKKNSDDTKPLINITKNXQDNIPXKTGKYDYCANLXDCNRVSGLNIINVILYDFGVQFAKKLGHKDPKSLGSSLITIFNTLIKNVAKSNTKENVKYDTKKNKNGGPXXLLNNXKLKNPSLIAGAKKLWSVQLISRTMVLFINGVFLMQIFNVVLLIVLQIKHLETLEKQFVLICLLSLQLISIAVLGXMTFVLLGYYLLVSYYITKPLETHVHLGVSFNTGFYIFLVLMLLQVITTFTLVLMKKKKLQKKLHCKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIA9</accession>
    <name>A0A1E5RIA9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Heat shock transcription factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Heat shock factor protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g1166</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-binding transcription factor that specifically binds heat shock promoter elements (HSE) and activates transcription.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotrimer. Homotrimerization increases the affinity of HSF1 to DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032993">
        <property type="term" value="C:protein-DNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:sequence-specific DNA binding"/>
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        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Heat shock transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
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        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
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        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339:SF1">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 12" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="15">
        <location>
            <begin position="648"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="112"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="327"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="515"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="547"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="774"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="808"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="272"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="117"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="197"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="333"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="372"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="515"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="547"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="572"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="594"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="613"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="774"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="827"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="12">
        <location>
            <position position="697"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="16">
        <location>
            <position position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00084017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ86632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="847" mass="94047" checksum="0E91BC80061FF016" modified="2017-01-18" version="1">MSALPKKKQKSNEFIRKLYNILQENQTPNIIQWTDKGDSFVILNPNDFTTLILPTYFKHSNFASFIRQLNKYDFHKVKKNAIEKLNFKYGVNSWEFYNQWFKKGGDDELTMIRRKNNNNNNNNNNNNNNNNTTNEPSGTTTATSKTTGKPTSKPTSKASGIISNTNTNTNANANTNTNTNTNTSTNTNTAKKDTFMNNNKNNKITTNDNIDDKLQAQESSGKLTSNSSFLNSTNNNNSDNSSDNDEERXDSGALSMSVHSRTDIQNIITPRFSRVNKRLKDLEKNMDYLNNENRYLKLELQKMNSKYNTMLQSLIILQQCMPSNANSISKMSNANTHPNNTINNNNNNNNNNNNNNNNNHNNDHNNHNNTTSTEPNGFNFNTNTNIHMDSDSPNHFITGFANMQQPLLLNDNNVPITPGNFAIVDPLPMSASXSAKQTGEINNNNNNHNTSENVKNSLQTTQESMSIGNLTNNLNTDVSASVLPELSLHQNYSSKLNNMYNAAAINNASANANVNVNNNSSPXNKMNGNGLDEHIPTAQSFTQQNNHLHGTTSINSPPSSRDQQQQQQQQQAFSYQQNNAASHYNSPQLQSHYPVPPPIAMYQPPPPPPPPHSSSSSSTSSSAHPANMVYAPXQNQPFKNSSLRKGFHVLLVEDDNVCIQVCTKFLMKYGCSVDVASDGLNAITNLETQRYDLVLMDIVMPNLDGATATSIVRSFDNETPIIAMTGNIDDEDLVTYLQNGMTDILAKPFTKNDLYSMLVRYLKNRIPISQQNALKTQNPSHSPHTGQPSTEQQPLDPVQDVNMDAVTGTGEQLIKEEQGSXGLSAEGSLNQPVDTVQQIANAKNINP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIF9</accession>
    <name>A0A1E5RIF9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">glucan 1,3-beta-glucosidase</fullName>
            <ecNumber evidence="10">3.2.1.58</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Exo-1,3-beta-glucanase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g1012</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1-&gt;3)-beta-D-glucans, releasing alpha-glucose.</text>
            <dbReference type="EC" id="3.2.1.58"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.58" evidence="10"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RIF9"/>
    <dbReference type="FunCoup" id="A0A1E5RIF9">
        <property type="interactions" value="437"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RIF9"/>
    <dbReference type="InParanoid" id="A0A1E5RIF9"/>
    <dbReference type="OrthoDB" id="1293114at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004338">
        <property type="term" value="F:glucan exo-1,3-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050732">
        <property type="entry name" value="Beta-glucan_modifiers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631:SF26">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184758" description="glucan 1,3-beta-glucosidase" evidence="13">
        <location>
            <begin position="22"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ86373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="320" mass="34677" checksum="CAB356154BAE1261" modified="2017-01-18" version="1">MFKLSATLLLLAVHHLTQVLCIGELGFNLPATDNNGNCKTTDDWVSDLETVKTYASSVKIYAVSDCNALQTLGPAAEQVGSVKLWLGVWEVDDAHFEAEQSALSTYLPEIDSSLVNGFLVGSEALYRGDLTPTDLASKIQTIQSLISNIDDKNGKSYSGFPVGTVDSWNIFVNGTNNPAIEAADMIMVNAFPYWQGQDASNASYSFVDDIMQAVQSIQSIKGSDFWIEVGETGHPTSGTTLQGKDGMTSVPSVDNAETLWQTGICALRAYGINVNVFEAFDEPWKPLATADTGQVSDVERHWGIWDSNGSLKYNITCGFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN55</accession>
    <name>A0A1E5RN55_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Altered inheritance of mitochondria protein 36, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g937</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN55"/>
    <dbReference type="FunCoup" id="A0A1E5RN55">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RN55"/>
    <dbReference type="OrthoDB" id="4081130at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ88317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="250" mass="27869" checksum="DCD83ACA33F60DD0" modified="2017-01-18" version="1">MLRALLPKKSTQQIKNLATQGTVARSRFHIRSQRCNSTASTNAQKKQSSGPGIKKIALVGLIGTAIFVQAAESLEKSKPKNSFTEEEYNTYVMNGLKRKKLMLNPESFPPIKFLLNAQGATAQKKLSDDSAYVVDPVNVVEYYRSNETSKYEALLNEIFEKNGSNKDVLPEKLPQGMICMLIGRYIQDHTLSDKIESKSSIIIKNFPKNLKEAIKFENDVGNVKSIIIGESDAAQSDIVGYYDTVKKVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNI8</accession>
    <name>A0A1E5RNI8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative zinc metalloproteinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g829</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNI8"/>
    <dbReference type="FunCoup" id="A0A1E5RNI8">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RNI8"/>
    <dbReference type="OrthoDB" id="74460at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.100.10.30">
        <property type="entry name" value="Jacalin-like lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001229">
        <property type="entry name" value="Jacalin-like_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036404">
        <property type="entry name" value="Jacalin-like_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053002">
        <property type="entry name" value="Metalloproteinase_M10B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021917">
        <property type="entry name" value="Unchr_Zn-peptidase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21054:SF2">
        <property type="entry name" value="MIP04191P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21054">
        <property type="entry name" value="ZINC METALLOPROTEINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01419">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12044">
        <property type="entry name" value="Metallopep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51101">
        <property type="entry name" value="Mannose-binding lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51752">
        <property type="entry name" value="JACALIN_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Jacalin-type lectin" evidence="1">
        <location>
            <begin position="534"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="685" mass="75555" checksum="61C719974944887D" modified="2017-01-18" version="1">MSDIELINFDNGMEAHSPCVIVHGKINGGSDVKDFQQVNVSSLQTPPLQYEINGAFFKAVVYLTPGLNRLRFTSNGKAGKTLVKTLALTYVPLLQDIPVHLCVLVAKDSPLLFDSPEEQKRREGGNNLQLAVKKLQMGALLMQAYTNEQMFRNGFGQRTFPFVQDWSLSYIFNQEGPMGRLRNSVKIHILKSEKTVRELRDSNLAQQNEKGSDRGGLFGIAMDALQNYNNGQLLQNNVEKPLQAAVMFLDAHWDPKLKLILTHAALGGGTEEIKLAIFGSHGLYSWPSCMEDITRYFLDPTKIDANAVANDNGECGSYWECLCVTLGAFLHEIGHSLGCPHQEYGVMLRDYPVFNRSFVSREAASQRPSRQLLAPPIYPKSECQWHRLDVIRFLYHPSFTLPEDYEDPSFMRPGKLAQFRYSAPSILPNANSKTKGSFTLKSETGIYLIEIITKQDDLCHAWFEYLPLSVGGCGPQQEVTLTYQQLVDKLPPQYSSSSKDIKIKVLSVNAPEFSVDNLEDLVLNSSVTVNGMSMVKGPLLGLPASKENPPFVVPVDIANVVSVRVYHGGALDGVGFTYAKGNSSGFFGKTEPDVVFGNKTGSYTDVLVRGNNGDPSEYIIGFNVRSGQWVDAIQVITNKRGVISPMLGNANGGSQHNLMSPSGSKITGIQGKIGQWCDSIGLMYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNP7</accession>
    <name>A0A1E5RNP7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Autophagy-related protein 20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g892</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sorting nexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNP7"/>
    <dbReference type="FunCoup" id="A0A1E5RNP7">
        <property type="interactions" value="144"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNP7"/>
    <dbReference type="InParanoid" id="A0A1E5RNP7"/>
    <dbReference type="OrthoDB" id="289314at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06867">
        <property type="entry name" value="PX_SNX41_42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044106">
        <property type="entry name" value="PX_Snx41/Atg20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051079">
        <property type="entry name" value="Sorting_Nexin_Autophagy"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46979:SF1">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46979">
        <property type="entry name" value="SORTING NEXIN-41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="9">
        <location>
            <begin position="152"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="371"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="585"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="35"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="371"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="392"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="417"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="597"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="610"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ88508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="730" mass="82979" checksum="4FC1701A127E7B3F" modified="2017-01-18" version="1">MMNDLFGDDILEVPQLNSEDNKEIFDSSKNGENSHVSDSKKSLKNSQKEQSMKLNGDKTGGENHSKKQTNGKRDLESETTSTKKKKAPSNGKLNKPSSFSKPEGLSNLISFGDEDDTQDTNTKNKKPLDLNIQTNGSSSISKSHKFIGTGSFPHTVFIEEASKASSGQGSATKYVLYTIKYGEDTVKRRYSDFELLRKILVKLFPTTLIPPIPKKQGLKSYSQTITSTDRYKKTHTSYLLPVQEETNGIDLSHSVIRCGVKDEKLIRHRIRLLTSFLNRLLNMDEITKTNIIFDFLDPDQQNYVDLINGLPSISNNLLTTSIYQLNPSNPLCTDMKIYSVLPIPHKQKTAGEASTSFISDASKTLQHLYLSSSPSSSSTSLITPPTSDEDMNANTGVSASGHINSTEPLFENEEEQQQQQQQQQRNQAHQTSNPDFKDFDENMHNPLSSVLDQHFNKYEHILKLQIFKYNKKSIQHLKELQLEYASLSQLWGDFSGTSTYTRSSILLSAMFDTSTAFEESHLNMEILAGTLYYNISEPLYELCGLAQSAQKLIEYRNLKKTQKNMVAHLISSKKQQLEKLKLKQKTKTPNNTLDSNQQQQQQQQQQQQQTPQAPSNESTRQSSFYGNRLFKRWNQITTIVKETINYVEVDPEELIPKLENEIETLQSASRIAQQDLVEIENHINLVELPKFSRTMDTEIQILLKEYAKYMKEAASKNLKYWKAVKNDIQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNP8</accession>
    <name>A0A1E5RNP8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Actin-related protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g813</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNP8"/>
    <dbReference type="FunCoup" id="A0A1E5RNP8">
        <property type="interactions" value="751"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNP8"/>
    <dbReference type="InParanoid" id="A0A1E5RNP8"/>
    <dbReference type="OrthoDB" id="7340501at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10211">
        <property type="entry name" value="ASKHA_NBD_Arp5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.36.70">
        <property type="entry name" value="Actin, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="373"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="292"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="420"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="379"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ88494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="750" mass="86704" checksum="C941E0776F3269D3" modified="2017-01-18" version="1">MTYTADRSLSPLPVHSLNDAKIQIEPEPYDSESSFGKDIPIAIDFGTYSVKAGYTNKPMPSHDFSTLLSRWRDRKAKATYTFIGNDTDLDPSIKSQSKSPYDGSFITNWDYVEEILEYTFDHLSVQGSGGVPNPILMTEKLAAIAQQRSNWYQLLFECFEVPKVTFGIDSLYSFYQNTSTGNDTGLVIGSGHEETDVIPVINGKGVIQESKRLNWGGAQSVDYLSNLLTLKYPYFPDKLSNYQFESMYKDFSYISPNYHEEINNVLELDNLESKDIVVESPFQEVLLPTKTEEELAAQAERKKQLAKKLQEQARVKRQEKLIQKQEEYEYYSEVREKWKSLPKAALLKELTSAGFDDEADFKKYVSGLEKALARARQQEDDENDGPEEPEQEPQFELVDIPDTELNEDQIKEKRKQRLMKANFEARKRMKEEKLKRLQEKEEAERRDMEWRKSNLKEWVHDKRSKLAVLLKSRKDKLKMKEDMKDRKSAASQMRMKNLASLADDVAIGNKRTRQQATIDNDPNDTFGTNDDDWAVYRDVASNPELLDEILDEEYKKILELEQALLEHDPNFTEEDTVDAQYDWRKSTLHLFLRGPRPHDSEDPHEQHQLHLNVERIRVPEVLFQPSIAGLDQAGISELSKRVLLGKFGARSNQLSEVTKRMAQNVLLTGGNTKIKGLRERVVKEFTEFLPVGTKLHVYNASNPELDAWRGMKKYAESTDYQNSFVTKKQYEELGSDYIKEHKLGNIKYYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNP9</accession>
    <name>A0A1E5RNP9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g965</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNP9"/>
    <dbReference type="InParanoid" id="A0A1E5RNP9"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184824" evidence="2">
        <location>
            <begin position="19"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="189" mass="19461" checksum="375C51537BB7D31D" modified="2017-01-18" version="1">MYFIHFLTAIALFQTAFSKTIFTDSSIITNTLFSGTGNVNYAAEITPTADQSTFSFPTNELIMDSSYAASTATDAASDYDEGAYAGDMYNTAPSNATTSSISTLAMSSFASNATNTGILTSAPTSSAHSNSSLADTATVAPSTNTSSTFSQSHSKSNTTSVSSKNGGVTNNGGVMNVFAFVTALMLALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP51</accession>
    <name>A0A1E5RP51_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MIP18 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g737</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MIP18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP51"/>
    <dbReference type="FunCoup" id="A0A1E5RP51">
        <property type="interactions" value="413"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP51"/>
    <dbReference type="InParanoid" id="A0A1E5RP51"/>
    <dbReference type="OrthoDB" id="2746at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.130:FF:000004">
        <property type="entry name" value="cytosolic iron-sulfur assembly component 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039796">
        <property type="entry name" value="MIP18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12377:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR ASSEMBLY COMPONENT 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12377">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR ASSEMBLY COMPONENT 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="246" mass="27249" checksum="161BE2EF185ED571" modified="2017-01-18" version="1">MSEFINENPNVLDSSQLPKRKVEDAEGELLHSSDITPNFASCSQWKQLKNIITDEESNIFKVFNTTSALLRLQNNIAKKTIGSDDILADGIAKESSPSLGDLAKSFKNEETDPRVDLLDSQEIYDLIAHISDPEHPLTLGQLSVVNLQDIEVIDRSSAQTKMGEVVVKITPTITHCSLATLIGLGIRVRLERSLSPRFRITILLKKGTHQSENQVNKQLNDKERVAAACENDQLLGVISKMLSTCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV99</accession>
    <name>A0A1E5RV99_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">U5 small nuclear ribonucleoprotein 200 kDa helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g411</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RV99">
        <property type="interactions" value="1332"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV99"/>
    <dbReference type="InParanoid" id="A0A1E5RV99"/>
    <dbReference type="OrthoDB" id="3971303at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003287">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein 200 kDa helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000012">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000024">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000001">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000062">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041094">
        <property type="entry name" value="Brr2_helicase_PWI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048863">
        <property type="entry name" value="BRR2_plug"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF4">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21188">
        <property type="entry name" value="BRR2_plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18149">
        <property type="entry name" value="Helicase_PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039073">
        <property type="entry name" value="BRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="516"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="732"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="1364"/>
            <end position="1540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="39"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="11">
        <location>
            <position position="1658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ90769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="2172" mass="247051" checksum="EA792C243AB2153A" modified="2017-01-18" version="1">MSEQPNNVTEGDKSRIKELYRGDTMTKSISKVDRKLLDAKHTENEDELEKLKPKSLNGEVTMRSMGSALQQDASMKRTAYATYSSSLRSTTEHRRQSKSNAHRKEEKKEKEEEEEEVFLSYYPSNNNSQEVYDQIMHWVLVHLFDNDATHTAVLTTVDYLLKELLGEQKADEEFEQRTAAKKARIEAELGVRFKNDTFHQLLDMVKQITDYGKKHDRGTEIAAIVDFSDYDKEEEDEEGDEVVHLDDKQLGRTYTEGKEDANNNEIEFDFDEESEEELIPTAELQEKQRNATVETANVEYFEALGSFHDMSKPHIERFLLANLNRVDVAELTDKTITIIAQSSNEQETEKRLTEIYAFDEIPIVQTLLQYRLNIKWGLKYAEAKEKEVVLTEMHQLGLDHLLTQHSKKRANENLDGDEGPLSKKLRHXIPTSKLRIIDLGSFKVEPVKTINTKVQLPKNSYKKIKDHYEEIHIPAPDPLNDSYPLVQIDELPKWALRAFPQSETRTLNRIQSEVFPAAFHNDFNILLCAPTGAGKTNVAMLTILRALAHFVDEETGNVDLSKVKIVYIAPLKALVQEQVREFGRRLDYLGVKVVELTGDSNMTRAEIKSAHILVSTPEKWDVITRKSSNFKLTSTVRLIIIDEVHLLHDERGPVIESIVSRAAHFKTRLVALSATLPNYTDVAHFLNVEKGKGMFFFDSTFRPCPLAQQFVGVSQTSPLKKLQAMNQACYDKVIESVLDGKHQVIVFVHSRKDTAKTANWIVEKLKEEEKELSFLLDQHEASSAVLEKESETVHDKDLKHLITHGIGIHHAGLHRNDRSLAEDLFADGLLKVLVSTATLAWGVNLPAHTVIIKGTDVYSPQLNQWVQLSPQDILQMLGRAGRPRYDTHGEGIVITNKEDVQYFVAVLNEQLPIESQMMGKLVDAVNAEIVIGNIKNIPDAISWLQTTYLYSRMLSDPQLYEVYTDRATAEENLSAYCEDLCHSAFTYLQEQELAVYKAVEGTIHATILGRISSFYYLRHDSVANYNTSLEKTFSLVELFSVFGQSAEFQYMAVREEEKYELKKLMDNTPIPVKDSLDEPSTKINVLLQSYISRLKLDGFALNADMVYINQNAGRLLKALFEIALQKKWSRISKLLLTTSLYVSRRMWLNNSPLRQFFTCPAEAITRMEASPLPFKAYLDLKTANEVGQALRLEKYGKTVYDLLKRFPKLKVSCSVQPITRSILKFDIELLPDWTWDSKYNGSVEKFWVFLEDVNGDNILYQDILNVSESSLGVESAITFTIQLPNDLQERLPPNYYISVISDTWLQCEYKIPVIFKDIILPKKFPSFTTLSNETLYGTENLNNGEFEKMFDFRNFNRMQSQVFETLYNSNENVLLCSSKGTGKTSMAFVAFLNHLRQNKGRAVYICPDQLKINHLLGLWKTRLSSLQDERKMSQFGSDAKLNIEIFSKNHLVLATPFQFESFTRRWKYRKNLQKLDLIIFDDAHEVSNPIFGSIYESLISRMVLMHTQLETNLRIVSLSNTLSNARDFGEWMGIPKTNIYNFSPTEKEGELIVHLKTHDSETIHIEHDLMCEEISSLVRAHVTPLVFVANRKDAIEMSKTLXLSLKKNDVASCMTEFSEQSLDDITDSLLKRSLRSXIGILYKGMNSKDLSTVLQLSDKLQVMIATKDVTALNIHSSFVFIRGTSYFQASENRSLDYXLNSLLEMVGCCSYNGAENNVGNVTILTTEKTKQYYSKFLSEGEPLESFFPFNLGEFLLSECSNGIVTSKSDIMEWITYSYFYRRIHANPSYYGVKDTSSIGLSAYLTELFEGVLKELAEARLLTIEDSELGTSTSEGPTENEESDPALVPSAGAVISSHYSLSLTTTESFMSNLPKINNISKFLEVLALSSEFDSLLTRSEDSSALYNLYTKLPIKITDFDSFHLSSPGFKVFVLLQAYFSRAPINADFQSDLKDILVIALRLVNAMVDFLSLYGKLSALLLMEFSQMLVQGCWHSDSPLAQIPHFYGNATLLEKCQEKHVDSVYGIMELEDEDRNEIMGFLDSPSKLAAVADFVNNYPNMEIECTTNIXDGVGNANESXAASITLTRDDEPESLEAVSERYPDXKREEWWLALGIADSKTLLALKKVVMDKESKSFTLPFSLEKPGAYTLTAWAICDSYLHSDKELSFDIXIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVJ7</accession>
    <name>A0A1E5RVJ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g497</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVJ7"/>
    <dbReference type="InParanoid" id="A0A1E5RVJ7"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="96" mass="10336" checksum="A2B53B4C5EB89F9A" modified="2017-01-18" version="1">MNYLVLSSDASIPYVPQQQQQQQQQHSVNQLIPQQQVTQQELLLGNGGNVHARNNSISSVNSVESATTATTTTTPSINASTTSSQGNFDIKPLVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVL4</accession>
    <name>A0A1E5RVL4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein PET122, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g444</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RVL4"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070131">
        <property type="term" value="P:positive regulation of mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008732">
        <property type="entry name" value="Pet122"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05476">
        <property type="entry name" value="PET122"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ90914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="295" mass="34949" checksum="48CD8980E351E5A2" modified="2017-01-18" version="1">MKGLIPKETEKRLTHLLYNNQVAQAVNVIRLAKSEYQLNPSVGLFKTCLAKSIENGYSNGVHFLFDKWIVKDHYYHNQDNDLEQLFPKMAYVTMNKXNDNFVMTERIIEKYKKIFLDKHNNXQSCGNEHVFYLMQLLQVENTCKHSLXRYTKQSFKELWKLYLKEVDQKFNFDSGLAKDILSAKDFPHLGRSIKKCSLSSLYKFVLHIKYIEVKNINSFRLLLTLVLMESNFSLYEKTVLLEKFQKVFENYKDIINYTEPLKVMESLKQNPKLLVNXELNILKRIKHAKEEEIDX</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVY1</accession>
    <name>A0A1E5RVY1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">S-methyl-5'-thioadenosine phosphorylase</fullName>
            <ecNumber evidence="4">2.4.2.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">5'-methylthioadenosine phosphorylase</fullName>
            <shortName evidence="4">MTA phosphorylase</shortName>
            <shortName evidence="4">MTAP</shortName>
            <shortName evidence="4">MTAPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MEU1</name>
        <name evidence="6" type="ORF">AWRI3579_g343</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>S-methyl-5'-thioadenosine + phosphate = 5-(methylsulfanyl)-alpha-D-ribose 1-phosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:11852"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="EC" id="2.4.2.28"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.28" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVY1"/>
    <dbReference type="FunCoup" id="A0A1E5RVY1">
        <property type="interactions" value="407"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVY1"/>
    <dbReference type="InParanoid" id="A0A1E5RVY1"/>
    <dbReference type="OrthoDB" id="431409at2759"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00873"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017061">
        <property type="term" value="F:S-methyl-5-thioadenosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09010">
        <property type="entry name" value="MTAP_SsMTAPII_like_MTIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01963">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010044">
        <property type="entry name" value="MTAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679">
        <property type="entry name" value="S-METHYL-5'-THIOADENOSINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679:SF2">
        <property type="entry name" value="S-METHYL-5'-THIOADENOSINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 4" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="5">
        <location>
            <begin position="20"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="78"/>
            <end position="79"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="238"/>
            <end position="240"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="4">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="4">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ90989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="325" mass="36501" checksum="9BCAFA6914BF836F" modified="2017-01-18" version="1">MTGPSARSNTTAVPPYEIDLAIIGGTGFYHLDNNPDLKQVLKFVETIHETTTPWGKTSSPVTVYKTVTTGKYIGFLSRHGLHHEYSPTTVPYGANMALLKHLKVKAVVAFSSVGSLKEELKPGDFVIPDQLIDRTKGVRQSSYFNETGLVGHVSFGNPFCKELGQFLHALGKEIDNHEVHYNGTLICMEGPQFSTRAESLMYKSWGSEISVINMSCVPESKLARELELPYQMVCMVTDYDSWRLEEHPVTIDEIKKTLQKNNDNAFKIVCKIVTSLDQNGSPEFMANNPYKGHTQSSISTKPTRDPKHINEQKIKELNYIFPEWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZN9</accession>
    <name>A0A1E5RZN9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g56</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZN9"/>
    <dbReference type="InParanoid" id="A0A1E5RZN9"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="19"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="172" mass="19973" checksum="51DAC1493A1D0D19" modified="2017-01-18" version="1">MDDSFQIQQQQRLVQKWNLIAYGVSLFIYSFNIFRVHSVPGFVLRCFVYGYYLVVLQLELSSFLPTWKKVNMNRLLKNCSFLESFVGRGFVNLLVTMIASRIRLTRGFFGEIAFLGNIAFSIGLITCEFKKNNSFPKPLSYNIREFMIDEQDIITNSEERFSENISDNSGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S077</accession>
    <name>A0A1E5S077_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g43</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5S077"/>
    <dbReference type="STRING" id="56408.A0A1E5S077"/>
    <dbReference type="InParanoid" id="A0A1E5S077"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014805">
        <property type="entry name" value="SKG6/TOS2-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08693">
        <property type="entry name" value="SKG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="156"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ92459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="172" mass="19839" checksum="E440CEF338E2F011" modified="2017-01-18" version="1">MNIVSSILLAKRSDSSECTGDKYECQKPTDSSYTKTIVLAVVIPVAVVIVVFSFIVLHIYRKNQREKLEDYDPEFVGGLDYYERKYYEPHMMTDQQQQQQQDPNRENYYNEAGTAESISDLHNLNVETGFQGTNGDRVNYNEMHDININTPSHTSNNIKNNNNNNNNYNNNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S0G4</accession>
    <name>A0A1E5S0G4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g69</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5S0G4"/>
    <dbReference type="FunCoup" id="A0A1E5S0G4">
        <property type="interactions" value="58"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5S0G4"/>
    <dbReference type="OrthoDB" id="4068767at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="375"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="219"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="455" mass="49570" checksum="D599684E54E09F6E" modified="2017-01-18" version="1">MDPSQINKSTPSLIGMSVRQCDDVENSDYPEIVASNYHAHGNNGASSSSLDNPQTTNGNRPYSKDFLSNKDHFKKSSSFVNDSNNEYDYTTVNELDQEGALLTDEYLGTVDTENVVLKPMKSPHEDYNTEESKLALRKRLNLLKRRQSEVQNSVNQLSAASSNVEEPGASGTVTHKHDASDRITHSTGNSMHVTPVTSSSNTLATTTDPYEDVIDKPPHSSSSSSPSSSANNANAGIGQNGEEQIRNSYGEYILNKSHKPHLARGDSYQSNASDVTSSASTTDLLEHSLSSASLGACASGTVADRNAGDRVGRGHHQHVESNYLRELNRSLSRDPALKNKHIHAHDGAGADDDHSSSSTSVNNYHISKNDMEHAINSNSHNHTHAGNHHLKNEIGKKQTMNSTTGVSEWKIEEEEEEEEDNAADGQEKLDFEKEEGVLLNDQDGGMENKPDMLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0B0</accession>
    <name>A0A1E5R0B0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">26S proteasome regulatory subunit 4 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4484</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0B0">
        <property type="interactions" value="1024"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0B0"/>
    <dbReference type="InParanoid" id="A0A1E5R0B0"/>
    <dbReference type="OrthoDB" id="10255768at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000030">
        <property type="entry name" value="26S protease regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000007">
        <property type="entry name" value="26S proteasome regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000039">
        <property type="entry name" value="26S proteasome regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="12">
        <location>
            <begin position="214"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="14"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ80330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="436" mass="48665" checksum="B35FE2CA95F9A5AA" modified="2017-01-18" version="1">MGQGASRGKKKDKNQKPKYEPPAEYKSIRAGRRRKHGGAPGAEKLPAVYPTTRCRLKLLRMERIKDHLLLEEEFVANSELLKPFEKKEEEEKKRMEEIRGTPLTIGTLEEMIDDDHAIVTSPTTADYYVSILSFXDKDLLEPGCSVLLHHKTMSVVGVLQDDTDPMVAALKIDKSPTENYSDIGGLESQIQEIKEAVELPLTHPELYEEMGIKPPKGVILYGAPGTGKTLLAKAVANQTSATFLRIVGSELIQKYLGDGPRLVRQIFKAAAENAPSIVFIDEIDAIGTKRYESNSGGEREIQRTMLELLNQLDGFDDRGDVKVIMATNQIESLDPALIRPGRIDRKILFENPDLSTKRKILGIHTSKMSLSEDVNLDKLVTSKDDLSGADIKAMCTEAGLLALRERRMQVTADDFKQAKERVLKNKVEENLEGLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0B1</accession>
    <name>A0A1E5R0B1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP synthase subunit delta, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">F-ATPase delta subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g4466</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP turnover in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase epsilon chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0B1"/>
    <dbReference type="FunCoup" id="A0A1E5R0B1">
        <property type="interactions" value="733"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0B1"/>
    <dbReference type="InParanoid" id="A0A1E5R0B1"/>
    <dbReference type="OrthoDB" id="270171at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12152">
        <property type="entry name" value="F1-ATPase_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.15.10:FF:000003">
        <property type="entry name" value="ATP synthase subunit delta, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.15.10">
        <property type="entry name" value="F0F1 ATP synthase delta/epsilon subunit, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00530">
        <property type="entry name" value="ATP_synth_epsil_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001469">
        <property type="entry name" value="ATP_synth_F1_dsu/esu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020546">
        <property type="entry name" value="ATP_synth_F1_dsu/esu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048938">
        <property type="entry name" value="ATPD_C_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036771">
        <property type="entry name" value="ATPsynth_dsu/esu_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13822">
        <property type="entry name" value="ATP SYNTHASE DELTA/EPSILON CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13822:SF7">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT DELTA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02823">
        <property type="entry name" value="ATP-synt_DE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21334">
        <property type="entry name" value="ATPD_C_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51344">
        <property type="entry name" value="Epsilon subunit of F1F0-ATP synthase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="11" id="KW-0139">CF(1)</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATP synthase F1 complex delta/epsilon subunit N-terminal" evidence="15">
        <location>
            <begin position="25"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="F1F0-ATP synthase subunit delta C-terminal" evidence="16">
        <location>
            <begin position="113"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF21334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ80331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="156" mass="16828" checksum="A2C8D50F1FC00372" modified="2017-01-18" version="1">MFSRRLLLQAVKARTYATESAAETLKLQFALPHKTLFKNASVAQVNLPVQSGSIGVLANHVPIVEQLTPGVVEVFETLNGTPKKFFVSGGFANIQPDSVLNITSVEAFPLEEFDSSAIKSLIQEASKNVTSSNEEVATKAQIELDVLENLQASLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0D3</accession>
    <name>A0A1E5R0D3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cytochrome P450-DIT2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4514</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0D3"/>
    <dbReference type="FunCoup" id="A0A1E5R0D3">
        <property type="interactions" value="1484"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0D3"/>
    <dbReference type="InParanoid" id="A0A1E5R0D3"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11070">
        <property type="entry name" value="CYP56-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF223">
        <property type="entry name" value="CYTOCHROME P450-DIT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184548" evidence="6">
        <location>
            <begin position="17"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="4">
        <location>
            <position position="481"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="7">
        <location>
            <position position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="535" mass="60887" checksum="075242C407781AAE" modified="2017-01-18" version="1">MLFVLLAVYYIYFTPPWNFPSTIPTIPFYVSFLPFFTKCDQEKIYSTYLQKPMEKHGAVKIYFGGRWNILLSHPKYLATIFKKEQTYAKSGNHLKVSKSVIAAYTGENVISAHGKIWRVFRNTMTLGLTQYDEKIFFTNATLFSDMIKSKLTEKEMLKPSMDSTLTLIASTGQETAVQKKQKCMDFQKTANDDSSKTLDMLGCNAEQTNCKSGTVAMTDPIQRLALANISEAALGLDFGTLGNGDPELHLQLKLVKSQIFQPLYLTFPFLDNLPIPSRVKARKDVAKFRETLLAKVTSSLMNNFEYEQTNFASSSLIKAERNQQITKDQLVNNLVIILVAGHENPQLLLTSLFFLFGKHPCWQKKIRKEVQESDKSFNSIEKLPLLTAFIYETIRMYPPLSQIINRCTVKKTALDHNIVVPKGAYVGYNVYGTGRNGKVWERPAVFDPYRWGHSIDEITDNWRHCKQDCTMAAFHGGNRACLGEKLALAEVKITFVEMLSKFEWRISPNWRERMTPAGPLCPAGLELCFQTLDPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0I9</accession>
    <name>A0A1E5R0I9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g4524</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R0I9"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.960">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ80414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="254" mass="29080" checksum="3D43DFCF2A021499" modified="2017-01-18" version="1">MHGYLLPSRRNSPTKGQTFKDSVYTDSLLMKNWFSREIFEHPETTLKLTDSQNFAKNVSLWDLRHTDDVKCFRVIXYITVSHXRTMLLVRDLQNSFFVELTQKCISDFEICSGLRVTMLCKHESLMIQSSPLIDDIVLQAHLDFQFHGDKTDGSRIEWRPMKSVLGTQSDGLSSTISIFERTDGKVPNEDLETLVFPVLVLSKMTIASIETAQCTNIATKIKLNNNGPDAMAYEDSMHSDKKRYPLVYKICSCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0P2</accession>
    <name>A0A1E5R0P2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cell wall mannoprotein PIR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g4398</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PIR protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0P2"/>
    <dbReference type="FunCoup" id="A0A1E5R0P2">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R0P2"/>
    <dbReference type="OrthoDB" id="5415592at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005199">
        <property type="term" value="F:structural constituent of cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054508">
        <property type="entry name" value="PIR1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051153">
        <property type="entry name" value="Yeast_CWMannoprotein_PIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000420">
        <property type="entry name" value="Yeast_PIR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254:SF1">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00399">
        <property type="entry name" value="PIR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22799">
        <property type="entry name" value="PIR1-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00929">
        <property type="entry name" value="PIR_REPEAT_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50256">
        <property type="entry name" value="PIR_REPEAT_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184574" evidence="9">
        <location>
            <begin position="20"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="Cell wall mannoprotein PIR1-like C-terminal" evidence="10">
        <location>
            <begin position="300"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="88"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="256"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="117"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="150"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ80468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="384" mass="38812" checksum="A33DF85BA30AC001" modified="2017-01-18" version="1">MQYKKTTLATAALAATALSAYVPSEPWTTLTPTVTYANAITDYASSFGIAVNPITTGSLAPSASASASSKAKRAQAISQIGDGQIQATTATTSTTSTKQKPTTAAAISQIGDGQIQATTATTTHSTTSAKPRPTTVQAISQIGDGQIQATTSTTTPSSTSTKTTKTTANAVSQIGDGQIQATTATTSSTTTKTTANAVSQIGDGQIQATTATTKTTADAVSQIGDGQIQATTATTKTTADAVSQIGDGQIQATTSATASASATKESASPATSSKATSSTDPVTAESCFSNSTLAMTLKDGVLTDARGRIGSIVANRQFQFDGPPPQAGAIYAAGWSITPEGNLALGDEDVFYQCLSGNFYNLYDESIGAQCKPVQLEAIDLISC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0R6</accession>
    <name>A0A1E5R0R6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">aromatic-amino-acid transaminase</fullName>
            <ecNumber evidence="9">2.6.1.57</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g4602</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>an aromatic L-alpha-amino acid + 2-oxoglutarate = an aromatic oxo-acid + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:17533"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:73309"/>
            <dbReference type="ChEBI" id="CHEBI:84824"/>
            <dbReference type="EC" id="2.6.1.57"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.57" evidence="9"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0R6"/>
    <dbReference type="FunCoup" id="A0A1E5R0R6">
        <property type="interactions" value="276"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0R6"/>
    <dbReference type="InParanoid" id="A0A1E5R0R6"/>
    <dbReference type="OrthoDB" id="691673at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047536">
        <property type="term" value="F:2-aminoadipate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008793">
        <property type="term" value="F:aromatic-amino-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009074">
        <property type="term" value="P:aromatic amino acid family catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000074">
        <property type="entry name" value="Aromatic amino acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050859">
        <property type="entry name" value="Class-I_PLP-dep_aminotransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790:SF21">
        <property type="entry name" value="AROMATIC_AMINOADIPATE AMINOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="10">
        <location>
            <begin position="131"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ80480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="499" mass="55604" checksum="792583B6079721CE" modified="2017-01-18" version="1">MTNQAKSYAHLYSDEANARKPSPLKTCIHYFNDPNIVFLGGGLPMSTYFPWQSISAVSPKAPFVNGIGAPCTSSEDSIEYTIPKNISDTKGKDVIALERCLQYGFSQGQTPFLDFVREHTKIVHQMGKYEDWDVLATTGNTGAWESTLRVFCNRGDTILAEAHSFSSSIAAAEAQGINTFPVPMDNEGIIPEKLSEILDNWTEGVPKPKLLYTIPTGQNPTGSSLSVERKVAIYELAKKHDFIIIEDEPYYFLQTGEYVPADKRAPVTKAASHKEFLDNLSTSFISLDTEGRVIRMDSFSKVLAPGTRLGWLVGAKSILNTYLKFHEMSIQAPAGFTQTLVSETLHQWGQDGYLDWLIGLRYEYTLKRDFCIDSLKKHLPLDLMCKSDPTKPVIAINPPNAGMFFIVTVEAAEHPEFETKFGKDTKKVEEHVYEQIIANGALMAPGMWFECHYDGETEKNGKSVDTQVFFRGTFAAVPLEKLDLGISRVGDALRKEFAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0T0</accession>
    <name>A0A1E5R0T0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histone H3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4506</name>
        <name evidence="14" type="ORF">AWRI3579_g866</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2016-08" db="EMBL/GenBank/DDBJ databases">
            <title>Genome sequences of representative isolates from three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="10">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the histone H3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0T0"/>
    <dbReference type="SMR" id="A0A1E5R0T0"/>
    <dbReference type="FunCoup" id="A0A1E5R0T0">
        <property type="interactions" value="958"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0T0"/>
    <dbReference type="InParanoid" id="A0A1E5R0T0"/>
    <dbReference type="OrthoDB" id="842664at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000010">
        <property type="entry name" value="Histone H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00322">
        <property type="entry name" value="HISTONE_H3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00959">
        <property type="entry name" value="HISTONE_H3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="9 10" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="12">
        <location>
            <begin position="44"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ80505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ88329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="136" mass="15356" checksum="A6115FEB480AC67A" modified="2017-01-18" version="1">MARTKQTARKSTGGKAPRKQLASKAARKSAPSTGGVKKPHRYKPGTVALREIRRFQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAIGALQESVEAYLVSLFEDTNLAAIHAKRVTIQKKDIKLARRLRGERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0U3</accession>
    <name>A0A1E5R0U3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Vitamin H transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4509</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0U3">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0U3"/>
    <dbReference type="InParanoid" id="A0A1E5R0U3"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000065">
        <property type="entry name" value="Putative MFS pantothenate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF31">
        <property type="entry name" value="VITAMIN H TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="255"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="285"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="437"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="463"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="491"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="523"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="557"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="621" mass="71425" checksum="59AE55ABF7434098" modified="2017-01-18" version="1">MSTFANCKWIPQLRVLPEDLVDNKPTVISTPTEKNSATVATVGTTSSASFNEQSSVEKPTESNIESIDYDDLDSEAFFDKDTVVVDGGANHGKVVLQRGMPYELRDEKKSHKWYSFFNEFEYRCNKEYMKSRKWYQFLYPNHATSSPTERKLLYKLDIIVALYLLMLNWCXSLDTNNYTSAYVSGMKEDLKMVKNDYVNTSSISSVGAIVFQIPFMYFLPRVPPHILLPSMDLGWTAFTFACYRAKTLAEFRGYRFIMSAFGSAYYPVAQYIMGSWYGPDEISSRVFLFFCGQLLGGVTSGLLQARIYKSLNGVHGIAGWRWMFLIDAVAVSLPMVFLGFFLIPGTPGKCYSMILSDEEIKIARERMKRFSTKDGVPKKLPKFFSWATWKKVICRPTFWILVVFDTCSWNNMTAFSGSYTLWLKSQAKYSIVQVNNLSVLPACLGFAYVALCAFGSDLLRCKWLFLVFSNIMCVVSCAILIKWDVSSRTKWFAFMITYFSVAPSPCLWSFINDFLRLDPQVKAITWIAIYSISQSTYAWTPTLAWKTEDSPKFRTGYVVSLCFTIVYGLWTFVVLFFYKRNEKRNALINGIILYNDTNQQXVPEYVEEHLEKKGEYYYVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R191</accession>
    <name>A0A1E5R191_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL2m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g4191</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R191">
        <property type="interactions" value="463"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R191"/>
    <dbReference type="InParanoid" id="A0A1E5R191"/>
    <dbReference type="OrthoDB" id="268576at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000128">
        <property type="entry name" value="50S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.950.10:FF:000001">
        <property type="entry name" value="50S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="entry name" value="Ribosomal protein L2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_uL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005880">
        <property type="entry name" value="Ribosomal_uL2_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_uL2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022671">
        <property type="entry name" value="Ribosomal_uL2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_uL2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Ribosomal_uL2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01171">
        <property type="entry name" value="rplB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="RIBOSOMAL PROTEIN L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00467">
        <property type="entry name" value="RIBOSOMAL_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 11" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL2 RNA-binding" evidence="10">
        <location>
            <begin position="139"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL2 C-terminal" evidence="9">
        <location>
            <begin position="242"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="340"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="353"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="376"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="388"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00069872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ80660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="399" mass="44396" checksum="18C1E6AEE2D9C4B5" modified="2017-01-18" version="1">MLSGFVGAARMQQLRMPLAGATSNLSMLALQTRAFHKRNSTLLAQAEQTSTVAPPLGESSAAFKPNVLKIVPNETDISVLERQDELIKRRRKDAKEAGAMKKLKPHSPGLRWFRKAVNPHLWKGKPVKFLTMPQKKTGGRNNQGKITVRHRGGGHKRRIRLVDFNRMDSGQQIVERIEYDPCRSAHIALIKNLDSGKLSYILAADGLRQGDHVESFRNGIPQRIMSLMNGSLDSAILSVHTCQRGNCLPIRMIPIGSIVHNIGLQQFEGGKLCRAAGTYGRILDKIEAKKKAIVRLQSGEHRYVHLEACATLGVVSNIDHQNESWGKAGRARWRGIRPTVRGVAMNKVDHPHGGGRGKSKGKKLSQSPWGTLSKGYKTRKGKNQNRMKVKDRPRXKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1D1</accession>
    <name>A0A1E5R1D1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1D1">
        <property type="interactions" value="99"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R1D1"/>
    <dbReference type="OrthoDB" id="4069593at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="326"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="750" mass="85021" checksum="A4E7A235B16FA69E" modified="2017-01-18" version="1">MEHKAVKFNRYKNNDKDNNNSLIDQNQPQEHEKVIIDMCSKRMLDYESPIKKSLFSSQTKRXVLNSNKIQQDGESLLPSNNNVSKNKKVRRSFQLFLADVIKAREATQENVNQLYITEDCDFNQLQTRIPLRVELPPKLFVEDSTSNGLSLHSLQQHDAKRQSLIFDGTKYTNLDSRNFDDKTDESETSSSRNEPSDSGLLSDLNISIASACNEFSFEEKDLLKNKDFLGIDDQANVNLKQQCRNMLSKNYQKKRQCQSLQETENLAKNHKKHNSAELIPSNNHELVTIPSITDNSTTSMMHGTFKSNSFEQLINSFQNEYDMLNQAXSNNDYPTSITPKTKSNTEPQQSIKPESAADVMSSNFQFPASNCNSKKLEQPSTPNNKRYSHNRTKSVVSLDLLNVPLNAISTPPSPLFTKKRFDDLKEETPFQNNNKQITPPKRSPLRKSFSFEQETDTTKTCPFLSPEQKCTDQDDSFDSSMKYIPEQSCVILDANTLPYKPMTNYSSFNTTPSKQPANQINKVNFLSPSRQLSNNGYTETAYEKQHPASTHGVMSQTKIQHICEKGDDYESSYYDSVMSSTSLADNQLKGYVMPYNLMQQDHLTPNTQPSSVSSYVPQIVSPENVARESRNGELIGSKSKIYPQIPTSNDFLQGIEDFLQKCDSTKEVIVIDSDDEDDGDVQFVSEKIGPTRFSLETGTRSGELFTDDTLSHASPNLKKKKRMSKSHNIFQLCDETALQAKILAMELSND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1K0</accession>
    <name>A0A1E5R1K0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Box C/D snoRNA protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3958</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Required for box C/D snoRNAs accumulation involved in snoRNA processing, snoRNA transport to the nucleolus and ribosome biogenesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the BCD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1K0">
        <property type="interactions" value="127"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1K0"/>
    <dbReference type="InParanoid" id="A0A1E5R1K0"/>
    <dbReference type="OrthoDB" id="3972716at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070761">
        <property type="term" value="C:pre-snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048254">
        <property type="term" value="P:snoRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23023">
        <property type="entry name" value="zf-HIT_BCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051639">
        <property type="entry name" value="BCD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057721">
        <property type="entry name" value="BCD1_alpha/beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007529">
        <property type="entry name" value="Znf_HIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13483:SF3">
        <property type="entry name" value="BOX C_D SNORNA PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13483">
        <property type="entry name" value="BOX C_D SNORNA PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25790">
        <property type="entry name" value="BCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04438">
        <property type="entry name" value="zf-HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51083">
        <property type="entry name" value="ZF_HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HIT-type" evidence="9">
        <location>
            <begin position="8"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="58"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="359"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="367"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="450"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="10">
        <location>
            <position position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="477" mass="53984" checksum="9D519659AEBEF4DF" modified="2017-01-18" version="1">MSGDSKLCEVCQVKEFKYKCPRCFKKTCSLACSKKHKVENDCSGKQYDPQEYISSTTLKEHNQQVPASDTDVQDQTTKAQSAQSAGLAGPPGQTQADSTTVNNILVQRDYNFLINVNRQLSVLKNETWSRNKRMLLGQQRPEYTQIKKFKSNESDMELEFIHRRGCRCLLLPKGMKKSNINRSKWDKPLNCFVWTLEWVISGFELQEAEEKIEDEFAXETVNTIENTIDFITHKNKETHTLLNXVARQSRVIKYVLPKPPLQHGQEFPPRPSEQEVEEKLKNLIDQKNLKFYIKAFPKFISTVMDPKTVVEVDPSLTLSEILVHKTCIEFPTIYISVGGGNELKGYKIIDVLDELDANAPTSXQKKPKXETLKIHEPPVFMSKNANNSPTQESSSDSDEEPMETPIIKESISPKYGPDKTKNVPKSSILXEKVKSVTQSSILSEEVKXVSQSSILPDSDSNSDSDQDNGVTLDFLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1N4</accession>
    <name>A0A1E5R1N4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Histidine permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3878</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1N4">
        <property type="interactions" value="173"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1N4"/>
    <dbReference type="InParanoid" id="A0A1E5R1N4"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000001">
        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004762">
        <property type="entry name" value="Amino_acid_permease_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00913">
        <property type="entry name" value="2A0310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF13">
        <property type="entry name" value="HISTIDINE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="371"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="395"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="442"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="470"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="9">
        <location>
            <begin position="42"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="544" mass="60351" checksum="B4A9AB80D97683EA" modified="2017-01-18" version="1">MKVFEKKKDIYVEENCDSEQTAFIDKNAENTKRLNKDLDIRHLVFLAVGGSIGTGLFINSGSALNLGGPASLVLDWGLVATCXITVMNALGELAATYPVNGGFNVYFDKFLDPSVAFAVNINYVLQWAVLLPLELVAASMTIQYWNDKLNPDIFVAVFFAVIVVINFMDVKIYGEVEVVLSMIKLLAIVGFFILGIVLICGGTPEGHYIGNKYWREKAFVGAPGVPRFKNICSNFVVAFFSYSGVESCCLAAAESKNSHKTLPKSSKRTFWLVTLSYMSILTLIGFLIPYDDPRLLGGSNSVDATASPLVIAIENGGIKGLSSLMNAIILIAVLSVANSSVYACSRSLASMAQLGNVPSLLGKVDKRGRPMNAILVTLFIGLLSFVAASDKQEEVFTYLSALSGLSTLFLYFSINLSHLRFRYAMKVQNKSLDSLPYKSMTGIWGSVYGCVVLVLIFIASWWTSLEGTKIFFQSFASLFVLIGSYLGHKIYSRRWTKYYIKGKDIDLDTGKRDIDEEVFKQEILLEKQNYASYPWYKKFFEFWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R225</accession>
    <name>A0A1E5R225_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosome assembly protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Notchless protein homolog 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Ribosome biogenesis factor RSA4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g4269</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NLE1/RSA4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R225"/>
    <dbReference type="FunCoup" id="A0A1E5R225">
        <property type="interactions" value="788"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R225"/>
    <dbReference type="InParanoid" id="A0A1E5R225"/>
    <dbReference type="OrthoDB" id="10267436at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000092">
        <property type="entry name" value="notchless protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012972">
        <property type="entry name" value="NLE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF0">
        <property type="entry name" value="NOTCHLESS PROTEIN HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08154">
        <property type="entry name" value="NLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NLE" evidence="10">
        <location>
            <begin position="32"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="132"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="175"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="219"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="268"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="396"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="438"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="480"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00068030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00077034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00080836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ80927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="513" mass="56910" checksum="3CB317CE4BBEEE83" modified="2017-01-18" version="1">MATVLPPPSKKQRREAQQAKDIDLIPQDLPNVLVKFQAFDTGESVGGSIRVPGNISEKQLEELLNNLQGNTDEPVPYSFSCEAKDTTVDIQDNLYTSILKPGHKTTEDFLTLVYTPRAVFKVKPVTRSSSAIAGHGFTILCSAFAPNTSSRMCTGSGDNTARIWDCDTQTPLATLSGHTNWVLCCSYSPDGKMIATGSMDNTIRLWDSLKGESLGSKPLRGHNKWITGLSWEPYHLVKDGESPKLASSSKDGTIKIWDTTRRVCLYTLSGHASSVSCVKWGGSNIIYSGSHDKTIRAWDMNQNGACINVMKSHAHWVNHLSLSTDSALRVGGFDHSSSKINLNTVDEKKLKEKALQNYEKVAKKNGAFEELMVTCSDDFTMYLWNPTKQNKPITRMTGHQKLVNHVAFSPDGRYIVSASFDNSIKLWDGRDGKFISTFRGHVAAVYQVSWSSDCRLLVSCSKDTTLKVWDVKTKKLSVDLPGHKDEVYTVDWSIDGKRVCSGGKDKMVRIWTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R233</accession>
    <name>A0A1E5R233_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GTPase-activating protein GYP6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3895</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R233"/>
    <dbReference type="InParanoid" id="A0A1E5R233"/>
    <dbReference type="OrthoDB" id="27140at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF27">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="63"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="401" mass="47328" checksum="9585FF0325BB7957" modified="2017-01-18" version="1">MDKEQLNQEKNSEMSGGALGSAQLFLKEHYKTLGELKQRGILGSELYGGAHPNLSEERSLLYDVNKPLRGAVWYGFLNNLSFLDDSASDVDETAQLMEVSHRVLANDEDLEVMDLDIKRLLLYNIYEREQECRDIEKILYNYCKQNGQMYKQGMHELAGVMYMQMRGIFYNGNGVITPSLETVYVGFNNYMNLQQFSFDEVELFNFKNFHFEPMLQASFPSISRCLDSKHKLNNMLWLLRWLRLCFLREFEELDHLLIIWDHLIMQNDNTSLKKSQQKYQSFMTFLLIIFLVYRSKEIIYKCQDQSELLHLFFHFFNTKFFHKKRLQAILXCCNGLVEGKDLNGGAELPNTTDIRNAIKXLVCNDGETSKDLDMDWFHMFFEIHDYNRLKLQQRLKDKFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R243</accession>
    <name>A0A1E5R243_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Reduced meiotic recombination protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4383</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R243">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R243"/>
    <dbReference type="OrthoDB" id="2507795at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018822">
        <property type="entry name" value="UPF0646"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10336">
        <property type="entry name" value="DUF2420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="245" mass="28365" checksum="8A7CB54C3B72FF5C" modified="2017-01-18" version="1">MDIDLPAQEIDNPNSLLQHPNIRTLDALNEDNEKSTSNTSEXFNXNSDDYREQQIRXMASELLQNKPPPVISVLFKNSELRMFRDDLTVEQEDXNEEPLPYIFEDTSCLNENGRVFFTDFRRRLSLIAPSAELDSKEIVIFVPALNITLNEDNMYNNSMCFKDIVTLFERFCHNSKLAKTKMQHSILLFQVNLQERFFVRFNELIEIMEHAGTFEQLGKFTNDEANPLLVDDDLEGDGDVEVISD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R251</accession>
    <name>A0A1E5R251_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">RNA polymerase II transcription factor B subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g4150</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as component of the general transcription and DNA repair factor IIH (TFIIH or factor B), which is essential for both basal and activated transcription, and is involved in nucleotide excision repair (NER) of damaged DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R251"/>
    <dbReference type="FunCoup" id="A0A1E5R251">
        <property type="interactions" value="540"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R251"/>
    <dbReference type="InParanoid" id="A0A1E5R251"/>
    <dbReference type="OrthoDB" id="5963at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070985">
        <property type="term" value="C:transcription factor TFIIK complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061575">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16573">
        <property type="entry name" value="RING-HC_TFB3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000037">
        <property type="entry name" value="Cdk-activating kinase assembly factor MAT1, centre"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004575">
        <property type="entry name" value="MAT1/Tfb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015877">
        <property type="entry name" value="MAT1_centre"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00570">
        <property type="entry name" value="cdk7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12683">
        <property type="entry name" value="CDK-ACTIVATING KINASE ASSEMBLY FACTOR MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12683:SF13">
        <property type="entry name" value="CDK-ACTIVATING KINASE ASSEMBLY FACTOR MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06391">
        <property type="entry name" value="MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17121">
        <property type="entry name" value="zf-C3HC4_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003338">
        <property type="entry name" value="MAT1_metazoa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="10"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="123"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR003338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ80981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="326" mass="38498" checksum="8C052186E5F4427E" modified="2017-01-18" version="1">MDEEERREMCPICKTDRYLSPNMRFLVNPECYHKICESCVDRIFSLGPAQCPYLGCDKILRRNKFKSQVFEDVGVEKEVDIRKRVFNVFNKTLDDFEGDQDEFNKYLEEVEDIIYNLDNGIDVVNVEEKLKSYEDMNRQLILANVERSRQDIMNFEQRQKFEKEMKMKKRSLERQIEQEERANAEWAKKEIVNQLSMGSSSDEVIENVKKTVKLKKSSARRKLEDLNKVLRNNPYTMLQNGKLRKKDVSKTPFTPFNGDRDLSKRYKLTTDIYNDPFIVQLEKRKDFVASGFQTSVVYDRLLTEAFLGLGCYISEEKKTQKSSLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2D8</accession>
    <name>A0A1E5R2D8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Respiratory supercomplex factor 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g4160</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    <sequence length="168" mass="18991" checksum="9B4A34505ECD7457" modified="2017-01-18" version="1">MSLPSSFDTADQNDLNQLVFHEKIIKHCKQQPLVPLGVLGTTGAIILAIKNMKNGDKRRANYWFRWRVGLQGATVVALVIGSLIYGGTSAAAKKTQDDLNREKAKKREALWIQELERRDEESKARRKKAQEARSKSQQMAEESSKLQDELKKLEQEIKLSAQGKSNKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
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    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
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                <person name="Lee D."/>
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            <dbReference type="PubMed" id="27856586"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </dbReference>
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            <begin position="791"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="824"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="845"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="5">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ81917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="930" mass="103200" checksum="338E6532F541EF47" modified="2017-01-18" version="1">MDESKPKNATNIMVGLSKKHADLLNDPSLTETNENYDYDYNHDYKYNYKDIMEDLNDIHGALRDNKHXLAGAANNNSNNHDHNYNNNTTESLSGNSVVITKPLDNDNSHSQLGIKDYIFSSGVPKNNTEKNVPASEGQSLEGTHPNNKSKPIPSLDFDKNGEENQIGIGNHSKVNDHEFSADHLDVIQQESKSRKNSLTGSSQTNNXGNXNRLDDTNEDSEDQAKSYQGDYSDSDFEEELEKRLLNYTKETHKDYVQEHKEGTFEKSSKSSNKFQKAKKKLKKPFQGFLEQTTSDTSSLSNTPSTSSSSAMDMSDSEGSSHYDEDHIITLPSDEQGFDYQHKDNAKDTADHTLTDIIPNNAKNVEDEVANNQGSDHEFSHQSTDEDYKDDSEDGDSEDEDEXEDEDEDEDEDEDDLIPPPPPPKELDPDKLYALYPFQGPDPSHCNLQQDEACFLLNDQDGYWWLVKRCSDGKIGFAPAEILETFPERLARLNCWKNEQMNTLENGTSADKGQEQKLQRLHTLNDSVDSKRGSPLGKDSSEFGLNDSEKTGSTNSVQNKNSDTNESESNSHSPLIEDVSAISLLSAEKNKNSGNNKGVHKTVSFNNVISYMESHGSSKNELDDNYTNGQEPSVMGTERLKFDGGSDADDDDRSEVVSDVSWNQMPSLHIGKVRKNEPLLNDGSTTALAISSPCKKQGENQETEDXDTQNSKALQSFDGNKSSKSNADELHTPTLEPVSFAETFKINKTPALSFANKSVDTLPEDDQLTQIFVAPPSVFTHSKKDSPFSNVSPSPSIGEYSPSSSEFTNDSPPIDTLPKFESRTGDTSTTAQKTLLDQTTDHLKSKPTQDISTNSMEQTSNDDTLTSFKAVSNISKITNSPTSSNSSFETPGLHPLIHKLYDPVFSKFDELLQKIEKSKSSLSLQSKENLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4S1</accession>
    <name>A0A1E5R4S1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Peroxisomal adenine nucleotide transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3659</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4S1"/>
    <dbReference type="FunCoup" id="A0A1E5R4S1">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4S1"/>
    <dbReference type="InParanoid" id="A0A1E5R4S1"/>
    <dbReference type="OrthoDB" id="446044at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015217">
        <property type="term" value="F:ADP transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052217">
        <property type="entry name" value="Mito/Peroxisomal_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45939:SF2">
        <property type="entry name" value="CARRIER PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G13870)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45939">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PMP34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="242"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ81880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="341" mass="38167" checksum="EC88C7DB31DE5B6C" modified="2017-01-18" version="1">MSSFESAFTAALSGTVSNTIVYPLDVAKAQIQTSGSASTFPPAAKQLQQDQKKANDDESILQCLIRIYKKEGFKGLYEGYGLSMGSGIIQTFSYWLIYTLIRKKYYKIVCQRHLKNEIKTGKTKSTLKYQVLLSAWEELLLNITSASLSNFVTTPITIVSTSQKTAPSKSLKDHVLKIYNKNKSVLDFYKGLKISLLLTCNPAITYTVFKKIQHLIIKSYSHASVADSAKHDYSIDDTSVRLSPRINFMCGMISKIVATLVTFPLILAKVNLQNPNAETPYNNIFDVLINFYKKFGIKGWYRGLMIQLLKNVLNQGVNSMVKGELGKLVLGLRKTLNHAKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4T0</accession>
    <name>A0A1E5R4T0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein THP3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3561</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4T0"/>
    <dbReference type="InParanoid" id="A0A1E5R4T0"/>
    <dbReference type="OrthoDB" id="199574at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045107">
        <property type="entry name" value="SAC3/GANP/THP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436">
        <property type="entry name" value="80 KDA MCM3-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436:SF4">
        <property type="entry name" value="LEUKOCYTE RECEPTOR CLUSTER MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="436"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="547"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="75"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="953" mass="107959" checksum="A89A2BCAA5F24AD5" modified="2017-01-18" version="1">MSSNEKTEPNVPFSGLPTKAAMKDQPKNVHTGNHQQNQLSSTPNNKGQPVPQKKQKKKPRKGKPQTNTTPATPYKPQQQQQEQNPPQQQQKPKKQSNMLPDSIPKTNQTESIFEEQNTNVPPKGKKQQNLTDNNANFNANGKPFLTSERKKLPKQGNSPSITSXPVASFPASENXVPLDLSEQEDITPQSQNSMHGNSTSHMLPTTNSLLPDASXPKENAYAYSQEQETQTPEAHSPQQDQKNKXFDDSQKNXQKTKKAHLSSKSSNNAVSMPQGKKKAHQKLPLKPPIALMNAARCVKWNDQVYQTLLNKQLHVFTPFVNWLNRVKLYKDLDQASPPIKAQIVEIVEQAYACKVVNKIDWQFQTIGGVLYLRSGQMINIPLHVKEEIRLPFMNPSTKLVKEALAIAEEAKRLNESHRKKDXSLVEQNNDVXEXXNFSKXRNLDPDTGYTSRENGELPXTSDGVKEFNLSETXSFXRXALMDLQNTPDVQRVGTPEPLXHSINSEPEGSLNMKIPXPSTNTSQAYVAPVDPINTNNYQYTAAMKLPESQKPETKTMNKSKPPVFESNRKRSMEDNTXELSKSAKKKKARLEKFKIPQASVSPSPTPNNXALPFYKEDYGNLNKISSKAAFYDKNXPLXGTNMNLEKSFLRLTSEARPENIRPVQVLLHSYEHVLSKYKSGEATYQYFCDQFKSIRQDLKVQLIETPFTIQVYSKHAEIALKNGDLGEFNQCQTSLVSLFGKHLGMVHSSLYIQHCCFLILYRVMMKDFNSVLKLKCMALMHKQERQELLEEGQPVDEKEHCLDIDLSNLAIKVSLKINDCIMANDYHGFFKLYKIVCKHDSSMNRDLPSQPSEISLVQLFVEQCLLQNMRLKSFAYMARSCHTLLLGYLLEYLAFTDVEQLLQFFHKLNILHSVKLQQNPDGSDNSVINCKECALSVWTTFDKSKSIDIKGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5E2</accession>
    <name>A0A1E5R5E2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Increased recombination centers protein 21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3597</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5E2">
        <property type="interactions" value="96"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5E2"/>
    <dbReference type="InParanoid" id="A0A1E5R5E2"/>
    <dbReference type="OrthoDB" id="432299at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004128">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051872">
        <property type="entry name" value="Cytochrome_b5/Flavoprotein_Rdt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46237:SF1">
        <property type="entry name" value="CYTOCHROME B5 REDUCTASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46237">
        <property type="entry name" value="CYTOCHROME B5 REDUCTASE 4 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="6">
        <location>
            <begin position="186"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="37"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="75"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ82088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="265" mass="29887" checksum="BF8293E8E923DFB7" modified="2017-01-18" version="1">MDRXFNPLQRAFLEQHKNKDGDSKQDMFMKPRVPVPASXQRSQFRKPKMTQTTAAAAPTDTHSNALGIARQPGCSTLPXPSASNSSXLEGPAGAAQKRNKVVLQPGHGPLDWADLQAKKGXTGETTMPALISIRDWTTFARDNEAYEHNLIPVLKQLAESFSVSNPPSAQALSKYPPILPWEFKPALKIRLEEVQKHNTRNDMWCVLNGKVYFLSQYLDFHPGGQDILMKYCNGKIATLMFNKYHRWVNYERLLQTFYLGDLLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5G2</accession>
    <name>A0A1E5R5G2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acetylornithine aminotransferase, mitochondrial</fullName>
            <ecNumber evidence="5">2.6.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3823</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 4/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.11" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5G2">
        <property type="interactions" value="290"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5G2"/>
    <dbReference type="InParanoid" id="A0A1E5R5G2"/>
    <dbReference type="OrthoDB" id="5419315at2759"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00109"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003992">
        <property type="term" value="F:N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000004">
        <property type="entry name" value="Acetylornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004636">
        <property type="entry name" value="AcOrn/SuccOrn_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00707">
        <property type="entry name" value="argD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002325">
        <property type="entry name" value="PRK01278.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF79">
        <property type="entry name" value="ACETYLORNITHINE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 12" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="10 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ82147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="438" mass="48375" checksum="923DB0F8F818B7B4" modified="2017-01-18" version="1">MLKNCNKNIWHDVLKCKKWYSSMSSTFITDANKTQVTTYARPDDLVITRGSGCYLFDDFHKKEYIDFTSGIAVTALGHCNPEIANIIYQQSANKIVHSSNLYHNQEALKLSKLIVSKTKEFDGQNDXSKVFFCNSGTEANEAAIKFAKKYSLTKSPSKTKFIAFENSFHGRTMGALSVTYNPKYRTPFGDLIPGVEFATLQDIMTKFQGKLDLAKEDVYAGCIIEPIQGEGGVFPVPVDTLVTLKSVLQNSKFDIPLIYDEIQCGMGRTGKLWAHSYLPKEAHPDIFTIAKALGNGFPIAATCVNDKINDALKVGDHGTTYGGNPLACAISNYVVSTIADKAFLSQVNQKGAKLMSFLKDLQSAYPDLITDVRGKGLMIGCEFKEAPTKLIQKCKENGLLVITAGKSTVRFVPALIIDDETLEKGLEIFVKSLKQVYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5G6</accession>
    <name>A0A1E5R5G6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Histone deacetylase</fullName>
            <ecNumber evidence="2 10">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g3834</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the histone deacetylase family. HD Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="2 10"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R5G6"/>
    <dbReference type="FunCoup" id="A0A1E5R5G6">
        <property type="interactions" value="1080"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5G6"/>
    <dbReference type="InParanoid" id="A0A1E5R5G6"/>
    <dbReference type="OrthoDB" id="1918432at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032221">
        <property type="term" value="C:Rpd3S complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000001">
        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003084">
        <property type="entry name" value="HDAC_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF10">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037913">
        <property type="entry name" value="His_deacetylse_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01271">
        <property type="entry name" value="HISDACETLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="15">
        <location>
            <begin position="38"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="399"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ82141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="432" mass="48842" checksum="2076D0BD97B0550F" modified="2017-01-18" version="1">MVFEDKPFEDIKIKPSEKRRVAYFYDADVGNYAYGAGHAMKPHRIRMTHSLIMNYGLYKKMEIYRAKPATKQEMCQFHTDEYIDFLSRVTPDNVQQFAKESVKFNINDDCPVFDGLYEYCSISGGGSMEGAARLNRGKCDVAINYAGGLHHAKKSEASGFCYLNDIVLGIIELLRYHPRVLYIDIDVHHGDGVEEAFYTTDRVMSCSFHKYGEFFPGTGELRDTGVGKGKYYSVNVPLRDGIDDASFQNVFEPVIGKIMEWYQPSAVVLQCGGDSLSGDRLGCFNLSMKGHANCVNYVKSFGIPMMVLGGGGYTMRNVARTWAFETGVLNNVILDEDLPYNDYFEYYGPDYKLDVRPSNMFNANSPEYLDKVLQTIYQNLENTKYAPSVQLNHVPKDMEDKGDVEQDTAEAMDTKGGSQYARDITVEHHGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7D8</accession>
    <name>A0A1E5R7D8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GDT1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3306</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the GDT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7D8"/>
    <dbReference type="FunCoup" id="A0A1E5R7D8">
        <property type="interactions" value="680"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7D8"/>
    <dbReference type="InParanoid" id="A0A1E5R7D8"/>
    <dbReference type="OrthoDB" id="442680at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015085">
        <property type="term" value="F:calcium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032468">
        <property type="term" value="P:Golgi calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032472">
        <property type="term" value="P:Golgi calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001727">
        <property type="entry name" value="GDT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049555">
        <property type="entry name" value="GDT1-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12608:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 165"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12608">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN HTP-1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01169">
        <property type="entry name" value="GDT1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="UPF0016"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017494012" description="GDT1 family protein" evidence="6">
        <location>
            <begin position="23"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ82817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="299" mass="32249" checksum="8157F39E2DFA6999" modified="2017-01-18" version="1">MSRYSKYSQLVSLAAMPLLAAAAGTTPLSSTTASSTTSVITNTKATAGNGETEDQSSAATSFFVAISMIVVSEIGDKTFLIAALMAMRHNRMIVFLGSISSLFIMTILAGVFGQAFTSFIPKRFIHLSASVLFFVFGYKILQEGLAMPSGAGVQEEMEEVEEELAIQDLNNVNNVREGGAPSGSLYNQSTHKFKAFTLSVKNSFNKYLSPVFVQVFVMVFLGEIGDRSQISTIIMASSGHYWHVIFGSIVGHAICTALAVIGGKYLATKISMKTITLSGAAFFVVFGFVYFYEFFTNSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7P2</accession>
    <name>A0A1E5R7P2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7P2">
        <property type="interactions" value="293"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7P2"/>
    <dbReference type="InParanoid" id="A0A1E5R7P2"/>
    <dbReference type="OrthoDB" id="4070347at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="242"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="282"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="509" mass="57236" checksum="3A49D0A0E7561DDE" modified="2017-01-18" version="1">MNHSKINFSHVMNPAQVNAIRDFCDKTTSILTFDQLLSTNSYIQMLLSTTETFAKDNELLEKISQKKDTAKNLGMKESDVELYSQLMKMYKDYLMKLCNLKREGNFLNKDLIKYDYLNSSIFPKILLNCDPNDVRTEYVQQLIEITKNTELATKFFSKKKAKSSXMDENVPTKTTSSIKSTAGTLTKKPKKEITTVKVSLKRSNPTTTEEKADKVELKKRKKIKTETDEXVKRRGIPVAIRTSSISTNPESESSATKSKKSPSPSLKTPPQESFALVKTKSQESSILPSSSSSSSSTSFSASPSILQPSSSNNSSNLKSILKKTEDNQDQTTTFESTKNEKSQKQLSFNTDSVTVFGHDLPKHGVKVAFKELKQALNNEKKASTPKEKLLVDPSRINVLTLAPFSDINCISQCDISELKGGVVKLQTNSKPEYRSNFRNFNKNLNKEHTEKEFHSQTEEVKEESDSINQPIVMPAFGQNQLLLLKDRGKLPYKQVPEVRPNAYPPVSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7Y5</accession>
    <name>A0A1E5R7Y5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R7Y5"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="352"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="168"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="366"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="411" mass="45702" checksum="9CEEBF821CFA6D5C" modified="2017-01-18" version="1">MSSHDQGLSLLQNYSFGKKNVQDDDANDLQLNLGNTATTTTTTTTTTNNNNNNNNSLCDMEMSRKKRKSSVNNKSLLYEPEVDYMENEVMGESIFNSLPSANKKQESTREFPYTNVSYANPSGRRRSTVVGTSIIAPTYKPRRNSLFNPLVVNERSLSERSTSERNLSENTTNENITTENATNENITNEARNSLRTRNSSLNQSRSSPLSYAFSQSVSPGNKTNTNYVYXQKEQQMLAEQADNLKMAMVEGAAQRKSVSNRTSPSLRRASISNMRRMSTARASTSEFPFHSFSGRNSNSPIGSITTPPLSKQSXTSSDTSFKTIQKNQKNHSFNMDMFEPAQFEDQETLEMPMSPRTTPRTALEASPRSPSHTSSHTSSHTSSHTSSHTSSHTSPRTCPQITLTHINTNTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7Z3</accession>
    <name>A0A1E5R7Z3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">GRIP domain-containing protein RUD3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3382</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7Z3">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7Z3"/>
    <dbReference type="InParanoid" id="A0A1E5R7Z3"/>
    <dbReference type="OrthoDB" id="425925at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019459">
        <property type="entry name" value="GRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000237">
        <property type="entry name" value="GRIP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18921">
        <property type="entry name" value="MYOSIN HEAVY CHAIN - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18921:SF2">
        <property type="entry name" value="THYROID RECEPTOR-INTERACTING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10375">
        <property type="entry name" value="GRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50913">
        <property type="entry name" value="GRIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GRIP" evidence="6">
        <location>
            <begin position="394"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="155"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="341"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="43"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="60"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="7">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ83030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="491" mass="55424" checksum="ABEFEDFFD353C848" modified="2017-01-18" version="1">MGKKKLQNKXQKNNDGVQXPASDNIEPVVDARIENPVSDASLEDSKQVLDDQKDXDXANGNEQTNKSKIATFGADSSQVESKSNQETDTGASDEAKEKNGTPIENGNGENVEPDEDSLFKDEQISQLTSERDAIQTQYNTLLSKLSGMKAVFQEMQSSKQELVVLRDQLHEYETQNLALKNKTSTLNTERNLLKNETQEFKTNNEKLKRDLRDKDDIIQSLKTQLSKVEESTERELEAMKIIKKDLLDRNEELRLLLENTKKDNELIVSEKEDLQVQIEEAEASRASLKHXAELLEKQNIEMSDKYMDLEKEKTTVEGNLKSKISELQTEMLEKDTLIAKYQSELQGLNKLSELKAQYEEESKNKSLQIGKLRHEAIILNEHLTKALSMLKRSSDSDNVDKELISNLFISFVSIPRGDNKKFEVLELIANFLNWDIDQKTQAGLIHGSSTLSSLATQPGASNASNTKSGKVSKTESFVSMWTDFLQKESGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R823</accession>
    <name>A0A1E5R823_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein EBS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3173</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R823">
        <property type="interactions" value="202"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R823"/>
    <dbReference type="InParanoid" id="A0A1E5R823"/>
    <dbReference type="OrthoDB" id="3972394at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018834">
        <property type="entry name" value="DNA/RNA-bd_Est1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019458">
        <property type="entry name" value="Est1-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10374">
        <property type="entry name" value="EST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10373">
        <property type="entry name" value="EST1_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Telomerase activating protein Est1-like N-terminal" evidence="3">
        <location>
            <begin position="105"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="DNA/RNA-binding" evidence="2">
        <location>
            <begin position="406"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="907"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1020"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="444"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1024"/>
            <end position="1046"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ83056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1127" mass="127197" checksum="DBD73D3FB17D2216" modified="2017-01-18" version="1">MRENDEQDQNKGAAVTAASGASTVRTGIQENDTCNDANKFNLELIETCSNISKQLQNILKVPHLLSDNEDNTMLQGFVLFQHTKLAQLLSLCEKSKAPSTNHTSKLLEFSWNFLHNPILKYFVKQKKKILVKYNITQDKPNLSNVDIKDSNIANTLNGQNQNKKLRENVQCIVELRKLNNKFNKLTKKFIEFYNKIMSEYLSYLPLCVDTNEKKSIHYTVQDSLDIXYLTKMCNTFGYLKYNNELTFFKKEYLPETNVYEDENKVSTVEKNYLNILKSIYHISLALAQIHKFTTLMESINYKIAQFQPVLNWCRVTEYISSGSELFMANYGKXTDHYMILSKCYLKAECFGYAIFYMIKTINTDNSSFAPNDLTSNNSMNAGNTVLALQNLHSVINLDSMTQFLVKLDDMNKDKNNRLIINKEIIESYFLYCWKLHFQENDNVTNNNDSSNLSNPKNKGKKATKDSQLYKCRWNTLLEKISIRYKKNIVPIYINLIICIGGFQILLNKSTSTNTSDENLNGDFHNKQSSSVSRERRFFSSLPGATQDYLRFCFQYINYVLENVIIKEFENGKSMKNESSFRKDYQYLAFVRVLLSWCKSSKLVLQYVHRNTKFNILITKLVNLLVQTNYQLLHHLQSPEATHRPKRDHLFVEDYQLQKLGCLQLNDFNDSQLIMSSQQKKLKISTKIASFLETQEVNTSTQNRGANSFYASRNIPNTMVISENDYKLVLKSLVYTTKKIVSGNLVNVVLQNNGKYTSMTVQEVVDSRSNSINEGPSGSKEIWGSSSLLESKENNASPLVYSGGSFTLSGAPESTLFNTKSVANSMNILTRTNNGAAFAESKTDVDLEYPAVLKSYAQSSSMTDFGDGMRSTSSHGEAVQSKFFSEXSTASLQHPQNLAGNQQFEQHQLHHSNVQNEQAKRSFSSLPNFNSMSSLNTMNYSGLNSPSQGSNVSYENFDPNTASGMRVFPNPSASRENSLSSLNANIWNIGRSSSNTALPNSGRSASNGGLLYTVRSPSAEGVLSPSESSIIPNPTTAPANFNRSANFGTHPETSTEKKNFGEPNVLGNVSSSGYGYNNYGYSSQMQMNSRNXSGLPNNISYSGESGNGNAGXFTYPSDHHQTQFTNYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R833</accession>
    <name>A0A1E5R833_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulatory protein UME6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3174</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R833"/>
    <dbReference type="InParanoid" id="A0A1E5R833"/>
    <dbReference type="OrthoDB" id="3973262at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF45">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN UME6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="275"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="80"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="171"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="238"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="382"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="115"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="187"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="238"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="386"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="440"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ83070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="457" mass="51627" checksum="499E77400FABE2FB" modified="2017-01-18" version="1">MRTTLYNRPLPNIFSSSPPNSSSVVTRTDTNKNDSVPYSRKHNKHHDSNIKNNYSTANSGMNSNVMTLLNAATILKNDQTDETNSNTENEDQDSQYDNNYENTMGIVNDYSTYNEEEKEKEKEKEEEKEXEXXDXKEGHLKLNHNSEEDYGVSIVDYIANGKTNANEMKLKYQSSSKKPTNKLLISKPKKVTKRKQKLPKKFVIVREGSTSSDNDEXNDKINNSNHLVQQTKNYSAETNHLSFSPTSSSQASDEKPKSKSSKLKSQGQGIRSRKGCWICRLRKKKCTEERPVCENCARLNLECLYDDDTKPDFISDPELKEKKLKQIRKRTKEAKRQSMSVKLKKRYWEKVESAKDKQPTQQKPLQLADITASSLPLTTAPLSSTQGLNAPTPTNLSASALKSIPRSSNTNLIHDRTGPQEKTPPVLPPIIKLSKKVSPATMNSKQASPMHQAETKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R871</accession>
    <name>A0A1E5R871_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Kinesin-like protein KIP2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3423</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R871">
        <property type="interactions" value="57"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R871"/>
    <dbReference type="InParanoid" id="A0A1E5R871"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF36">
        <property type="entry name" value="KINESIN HEAVY CHAIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3 4" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="6">
        <location>
            <begin position="275"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="64"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="841"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="64"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="105"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="844"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="355"/>
            <end position="362"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ83109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="865" mass="96634" checksum="BCAC41B2B8798710" modified="2017-01-18" version="1">MHQGNSATEKNSPSDNGSSAHTPGSLSDKSESCTFYPSNGFMSPDRNTHISEFQRKIHTRLQKLNGSHNGDSSFQGTRSNLGKTDIASSTDNRNVGTSSVQNTNTEEKFNSKEHVPSKFYSRPSYSPSHANETLYKRKPSSSTSSVSGALSSSSTTPSPYLIYKNSSASLLSGSGIFSGKQEEQEEQEELTTKDLNEISISMHDNTLTSSHFQNITHSGKKEAHTGKQFFDSINRVSLSDHGSSEQLQRRSSTIIKSFSSGTNRFSENEPQYKDNIKVYIRVKPGILNVNSAAQLAPPWFLNERENIVGHTDIGGFQFDRVFGPNEKTSDIFHDAVHGLVSKVFQQYNCTIFAYGTTGSGKTFTMTGDEDTNXEPGIVQYSVNEIFKKIHIMNGPDVPDTNQRFTVKVSYLEIYNERVHDLLTSQPTLATKHQSLGSAKHNYVKPDLKIRDDPEYGVRIIGLTEHPVENTQEVLSLLSLGNSXRKFNSTNYNIRSSRSHCILLVRLSDSMLQTVTTLSLCDLAGSEKASAPSFFSSSSSSSSSSAFHFENXRPFNSNTVPTTPRSSYASSVCSNSSSLHEKTNKEGSYINKSLLALSTVVSRLASSSTDHHIPYRDSKLTRILQPSLNGQSIIANICTIDTWNTQSWAENFNTLRFASRAKNIELTVNKKTRVKKGQNNDICSGTSEQEYIDLIEHLRYQLNEKSENLGNVKTQQXKDYPWNNKFVLXXLFEQSLSHLXFKEQEIATKKFKNLLETQXFASIPFTKSKQNDXXSVESTTYPENNPQDMHLLHQLLREQESEIIELNKMIQRKDKIISALQTLSESRRISVLSGVSDHKSDHFVQTDTSSDLDQRVHSANSSFMVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R898</accession>
    <name>A0A1E5R898_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Translocation protein SEC62</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g3321</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC62 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R898"/>
    <dbReference type="FunCoup" id="A0A1E5R898">
        <property type="interactions" value="143"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R898"/>
    <dbReference type="InParanoid" id="A0A1E5R898"/>
    <dbReference type="OrthoDB" id="200187at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031204">
        <property type="term" value="P:post-translational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004728">
        <property type="entry name" value="Sec62"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011553">
        <property type="entry name" value="Sec62_asco"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00869">
        <property type="entry name" value="sec62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12443">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12443:SF9">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03839">
        <property type="entry name" value="Sec62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="251"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ83127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="292" mass="33344" checksum="8BB515F065A4DE47" modified="2017-01-18" version="1">MSVAPPKNQKVKQVSPETPLNIAKNLRTHKELKQRKGLLQGNQVDFFRYKRFIRALKSPEYTKLSQKDPVMYPEIQTEDDAKACVILLIKAQILYPVIKLHSDELKEHGLLPNKEFPQLIISNKAALQDDDYYVWNYNKKSISFYLKAIGVVSAIFALVCYPLWPLKMRIGVYYLSYAALGLVVAFFGLAILRLILYLITLPLFSGKGGFWIFPNLFEDCGVMESFKPFYGFGESQCYSFVKKQARKQRKAKRAAQKEETEDTTAPKAASTTALEKKKPVTSSIKKIEDIPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R9Z2</accession>
    <name>A0A1E5R9Z2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NAD(P)H-dependent D-xylose reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3004</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R9Z2"/>
    <dbReference type="FunCoup" id="A0A1E5R9Z2">
        <property type="interactions" value="418"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R9Z2"/>
    <dbReference type="InParanoid" id="A0A1E5R9Z2"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000007">
        <property type="entry name" value="NAD(P)H-dependent D-xylose reductase xyl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="6">
        <location>
            <begin position="35"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="5">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ83735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="332" mass="37840" checksum="8AC60505E38E8E35" modified="2017-01-18" version="1">MPETTKLARTVKLNNGVEMPIVSLGCWKIPNDVCANQVYEAIKLGYRSFDGACDYGNEVEVGQGIKRAIDEKLVERKDLFIISKLWNSFHHPDHVEYGLDKTLKDMGLDYLDLYYIHFPIAFKFVPVEERYPPGFYTGKQDEEQGKFTFEKVTYLQTWKALEKLVEAGKIKCLGISNVRGVLIQDLLNGCSIKPQCLQVEHHPYLTQENLIQYAKNQEIQVVAYSSFGPASFLEMDVELAKSTPSLFEHQDIVSIANNHKVSPSDVLLRWATQNGVAVIPKSSKPQRLLQNLHFEDKLTLSKEDLNKISSLNKNLRFNDPWGWNNADIPTFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA00</accession>
    <name>A0A1E5RA00_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial</fullName>
            <shortName evidence="8">Glu-AdT subunit B</shortName>
            <ecNumber evidence="8">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PET112</name>
        <name evidence="10" type="ORF">AWRI3579_g3009</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Subunit of the heterotrimeric GatFAB amidotransferase (AdT) complex, composed of A, B and F subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the GatB/GatE family. GatB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="8"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA00">
        <property type="interactions" value="389"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA00"/>
    <dbReference type="InParanoid" id="A0A1E5RA00"/>
    <dbReference type="OrthoDB" id="1722066at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00121">
        <property type="entry name" value="GatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004413">
        <property type="entry name" value="GatB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00133">
        <property type="entry name" value="gatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004012">
        <property type="entry name" value="PRK05477.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="GLUTAMYL-TRNA GLN AMIDOTRANSFERASE SUBUNIT B MITOCHONDRIAL AND PROKARYOTIC PET112-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659:SF0">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="GatB/YqeY motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Asn/Gln amidotransferase" evidence="9">
        <location>
            <begin position="367"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ83739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="537" mass="61545" checksum="34AA525A7566D266" modified="2017-01-18" version="1">MLRCKRYASSWATANSNFKLLPDYKLKCGLEIHTQLNTPHKLFSMAENQPFQSMAEPNSRTSYFDVSLPGTQPNLNPEVVLYALKLAKSLDCKINLQSTFDRKHYFYGDQPLGYQITQHYNPFAKNGVMCLNKQFDGILENEHLINILQLQIEQDTGKTVYDEKDQISLIDLNRSNVPLVEMVTAPDFSDLKQIRAFIKKYQSLVRHLKVSTGDLETGAMRIDVNISVNDFARVELKNLPNTSSVMNSIKYEYMRQVEIIRNNEAALKLQQSETRGWTGVETVKLRSKETTIDYRYMPDSELPPLSLAPSIIEDVSSLIPELPEEIMKKLMTAPYNLSLKDAKILTVSSSGHDALYTNEGLLEYYLQTFHKYVSLYDRFLINDSKPRINYKLPSNWIIHVMLGNLNKLQMHLDDCKLTPEGLANFLQMINDNKISKTSGDLLLFHIMENNISSPDFAALISEFELNKIEQVDETELTEICSSIINDINDPKLLDQIKNGQKKNSLKFLIGQGMRXTQGKIDAKLFEKTFKKLLSIKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA24</accession>
    <name>A0A1E5RA24_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2995</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA24"/>
    <dbReference type="InParanoid" id="A0A1E5RA24"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184662" evidence="2">
        <location>
            <begin position="20"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="433"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="569"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="290"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="433"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="453"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="479"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ83748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="628" mass="63187" checksum="D51A150DD3151B19" modified="2017-01-18" version="1">MKLSSVLGLFAGATALAKAQSTDEVTDIFTVTKSLTITVTDNLDGTLVYPPSEVLAALTQETSATTTVDAQATTEVNDKKENRMKIQQKNFPKERCASTTTKYVTYTVTTCPVCTATTPAPVTTTKTTYITLNSCTDTDAVTNTYTQVAPNKKREALLKEEEKCKKTTTTKPAATPTPHTTSSTTCTEKKNVTPSTITKYVTSTVYTCSTTPSPTTTSCTETHSHGTSCTQHPGTTSFXPPTSSTPVVPPTSSTPVVPSSSFVSTVSTNSFVQSTTSLTSSATGPRYMNSTSTAPTSSSVVPPASSSVVPPASSSVVPPASSSVVPPASSSVVPPASSSVVPPASSSVVPPASSSVVPPASSSVVPPASSSVSISSEQSSVSTLAPSSATTSLQTIVTSSTFTKVYPTVVSGSTTTTTERFISTATITTCPLSSASTTTAQTQTYENSKEGFKTTNTETESASFTNASPSGAAPSGTETGAAPSGASPSGTAPSGTETGAAPSGASPSGTAPSGTETGAAPSETPASAANSDVTSISSSGTGVFTSFSTTVLASSAAASEQSVASASSSLNESSNSASATITSSSSSSPKTTASITTQQNAANHNQVSNGILFKSILTTGLFTFFMLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA40</accession>
    <name>A0A1E5RA40_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">A1 cistron-splicing factor AAR2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2992</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA40">
        <property type="interactions" value="516"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA40"/>
    <dbReference type="InParanoid" id="A0A1E5RA40"/>
    <dbReference type="OrthoDB" id="201752at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13778">
        <property type="entry name" value="Aar2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.550">
        <property type="entry name" value="Aar2, C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007946">
        <property type="entry name" value="AAR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033648">
        <property type="entry name" value="AAR2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038514">
        <property type="entry name" value="AAR2_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038516">
        <property type="entry name" value="AAR2_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12689">
        <property type="entry name" value="A1 CISTRON SPLICING FACTOR AAR2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12689:SF4">
        <property type="entry name" value="PROTEIN AAR2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05282">
        <property type="entry name" value="AAR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAR2 C-terminal" evidence="1">
        <location>
            <begin position="260"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="455" mass="52259" checksum="403236399A22AD0A" modified="2017-01-18" version="1">MDESNQKLDQGANIFFDSLTKLNQKNTYAVEKLIVGINTVFYFTLDISNNSSNVEGIKNIPCDTNAADAGHQLCLITFQHXHINSEEXDSMIYGYWLPLVEPVSHNSLQSKKINYGFMYATQTEKFIPYVISNADTANKRLVQVAPHMVSYDNAETVERHNLWTVLTTNIDTKLLENIICETQAIVFPELVPSMVDNXXYYHVDTSLTTEEEETQWNHLVDEKSGSAFLQNKQDSNQNSLLHYTXIKFKSLEAIRTSHKMNDYMDKTYYLRKIISSTVNGKNLLGELQLSYILMCAVGSYGSALQWHNLLELFQLSSQLEQCIDVXHLYKLLETQLKYLPNEYKEVFLNAEVFQRCFYHCPFSHPGLESMNHALDRELLAQSKQENNTNDREDGETARIVELDENNDLLGEYGDEDLVSINHTSSEEEDYVNDNSELCENYKGYTVASKVVYRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAK3</accession>
    <name>A0A1E5RAK3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">LEA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2966</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAK3"/>
    <dbReference type="InParanoid" id="A0A1E5RAK3"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.20">
        <property type="entry name" value="Apolipoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47372">
        <property type="entry name" value="DAUER UP-REGULATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47372:SF11">
        <property type="entry name" value="RE19971P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58113">
        <property type="entry name" value="Apolipoprotein A-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="197" mass="22170" checksum="6BD24215FDADA904" modified="2017-01-18" version="1">MFNQVRLVATRNAFRSPVLKMTVSRTFVATPSNRYTIKDTLHEMDKKAGKKFVSGIDKAEEAVHDAKDVAKKAGTKVKETLYQKSADGKELKDDLKDKASDLKEKATDKAXDLKEKTQDNAQDLKETAKDKFESASGKSEELKDAWKNKAEEAGDKAQEWKEDAQGKFNETAAQGKELKDDIKKDXKKDIKKTAKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAK5</accession>
    <name>A0A1E5RAK5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Elongation factor 1-gamma 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2497</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein biosynthesis; polypeptide chain elongation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAK5">
        <property type="interactions" value="1208"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAK5"/>
    <dbReference type="InParanoid" id="A0A1E5RAK5"/>
    <dbReference type="OrthoDB" id="249703at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03181">
        <property type="entry name" value="GST_C_EF1Bgamma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03044">
        <property type="entry name" value="GST_N_EF1Bgamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000006">
        <property type="entry name" value="Elongation factor 1 gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000142">
        <property type="entry name" value="Elongation factor 1 gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1010:FF:000001">
        <property type="entry name" value="Elongation factor 1-gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1010">
        <property type="entry name" value="Translation elongation factor EF1B, gamma chain, conserved domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050802">
        <property type="entry name" value="EF-GSTs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001662">
        <property type="entry name" value="EF1B_G_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036433">
        <property type="entry name" value="EF1B_G_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986:SF1">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00647">
        <property type="entry name" value="EF1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01183">
        <property type="entry name" value="EF1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89942">
        <property type="entry name" value="eEF1-gamma domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50040">
        <property type="entry name" value="EF1G_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4 8" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST C-terminal" evidence="7">
        <location>
            <begin position="86"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="EF-1-gamma C-terminal" evidence="6">
        <location>
            <begin position="251"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="219"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="227"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ83935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="412" mass="46698" checksum="24AC2AB942AC58C2" modified="2017-01-18" version="1">MSQGTLYTFPTPRSTILEALIKTYKIDXTVKAGAESEEFATYFPLKKAPAFVGPKGFKLTECIAIAYYLVNLIEDEKVRATLLGRNNTEKASILKWLSFVNSDVVGESVKYYANVKNLVPYNKKVVENASVKFDQYAQVIEQRLKEYTFLVSERITLADVFVGGAFTFFFSVNADAAWRKAHPAITRWFNTVIKSEPLVSIFAEFKPVDKKLEFVPPKKEKKPAAAKKPEAAAAKKPEAPAAEAPAAEKKPKHPLELLGKSTFNLDEWKREYSNNDTRPVALPWFWEHYNPEEYSIWKVSYKYNDELTLTFMSNNLVGGFINRLSGSVKYMFGCLVVYGENNNNGIIGAVMVRGQEFKPAFDVAPDWESYDYEKLDVSTEENKEFVNNMWAWDKPVVVNGESKEIADGKVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAS3</accession>
    <name>A0A1E5RAS3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">E3 ubiquitin-protein ligase substrate receptor MMS22</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2591</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAS3">
        <property type="interactions" value="522"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAS3"/>
    <dbReference type="InParanoid" id="A0A1E5RAS3"/>
    <dbReference type="OrthoDB" id="4068315at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035361">
        <property type="term" value="C:Cul8-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019021">
        <property type="entry name" value="Mms22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28122">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SUBSTRATE RECEPTOR MMS22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28122:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SUBSTRATE RECEPTOR MMS22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0675">Receptor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="487"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="544"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="489"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="548"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="569"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="1333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1603" mass="182664" checksum="E3A4DA2C133143CE" modified="2017-01-18" version="1">MPENGYASLNTSSDDGDVYDYNDVQLVIPESQLTYVDXFNQQAQVHTLNNVNEDLRLKYPDHNEDLQPATFDGLTNKFGTRNPINSLDSTNAPVSLNESSQFSNPANVQRQAIPSETENIPNTLSPVVETPPLRWALRKRKAIQKMPYSLDKIFHQELLKGHKLPDLQDIDASQIALRLPGLSITPAPLKTSTTPFWDYENEDSDDDDFEIDIPKISGKQGNTERFDSSDEEQFCSPSTHTLNERSDEEDLLHKVSHRARQSRLISDEESQISQDDRIRKQDFQSELAQRDTELVATIESDLEDDRGQSQSDDEVPEDIIFRGKVVNLKHGYKGLLPKAVWEAQLRKQYEKEKRASHKHISSKDRDPNXKGVAKKKKXNXLNPDKSHFLQELLANSSQEESTVSADLDPXVSDLSKLPYNSNEAELMEKLRSRIDLQYADAYHDNDRFSVTSSTSSNHNTFIYDEHKNIATLDDCSFETKNNIHMSHXKNSDKENEVTXHETSSEDSVMFQKEKKTMPDPNTIESAEEYDFTDYMLSRDYARAKPNDSLSSTKNTKPKSNSHVFHSKNSKSTQXQTKRLNGQQTKPKKLIQTRLAYAKDKSTCFSDGDSDVSGIESLKTRRKGRKDKTSFXKSANVLLKNTKKHXNLHSASLSTRTKAKRKKKIAEYFAFXDNSXVEDAHKKLAYYTTVVEAPGTAFTIGRASGALSNEAATAFQTEMHDDXQSXVFLGSPIFHAIFDDILYVAPDTISVSIGDSSLTVSAFSNDYLTSIKRVLVKVVKVGATDVELINLLNTLXQFFYHKNNTGLLEIVQAFQKEFTKKLVRVKEKAKPIHFYTLATCHLLLYEILLYSSVTPEFKTVITGLIIESLENYFCTYAXCYSDAVRANDPLILESNLILKLIVEKIDGSSSLWQRFSRLKISKTEIFETVVNFFPPPSDKPLWNLMVPQNDSYSEICEYVKFIQLGINACNWPITNEMILQFYGILKRRKFMDYKQELIXSPXYNVVGVAPILEANSIFNFFLNVLNLSTVTTSLLERITPLGNLVESENPSQLPNRLNLLLMLALKTGVDFENKFEQLLTXALATVGSLHYKLILDGLLSLYMRKDANGLTVKASSLPACYAKASSSYKPVFLELWKKFIYNLKQALSQFKGNKTNVLVSLSNIMLTEIREKVQTNYDVVCLLLDVICSNLSALEPKWVDQNIFQTLSFESNXHVFKYYCKVLSFLIENNYLTLWSALTYNPHASRLSKXSNFLFYESILKLCNDEASFLQVTPFXIKXIVQGIFEAHSAAYLQLVKQYFNKDNKITKQFVIHSVTSRYENICQIINFIGLSKDMNTISLLMQSLKSHCDSGNETGLCQELVTFINKYLIDMVKNNQIFHYLKQKYNISNTETEKSIFKDKLRGISSLEIRSELIVEEFSQVIQFNLNLQESIDKVXLAIKEIEAAAAFKMLTETIKCLVHIGTDIAYQIAALLSKDINCLLEEAFLYVNERSFLILLTFCCNXCCYFKQSFAAVAGNGGYPLEFTVEFKRLLKNMLFITENFHEAKILKKNINXFKSGHDQRPLLLSQMLLQRLKPLLNQKHTLPLSXEKIEYLKIEEKKIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAY2</accession>
    <name>A0A1E5RAY2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit J</fullName>
            <shortName evidence="4">eIF3j</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 30 kDa subunit homolog</fullName>
            <shortName evidence="4">eIF-3 30 kDa subunit homolog</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">HCR1</name>
        <name evidence="6" type="ORF">AWRI3579_g2964</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eIF-3 subunit J family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAY2"/>
    <dbReference type="FunCoup" id="A0A1E5RAY2">
        <property type="interactions" value="119"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAY2"/>
    <dbReference type="InParanoid" id="A0A1E5RAY2"/>
    <dbReference type="OrthoDB" id="20381at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.60">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03009">
        <property type="entry name" value="eIF3j"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023194">
        <property type="entry name" value="eIF3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013906">
        <property type="entry name" value="eIF3j"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08597">
        <property type="entry name" value="eIF3_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ84058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="277" mass="30748" checksum="26574F5F67F68972" modified="2017-01-18" version="1">MSWDDEDFEVPVASKDTPVLESWDDDFANTSDNEVLESWDAPEKPKKQAAPAKAPAQAKPSKKEVNNKNKNTEEKVLLEIDTLDPKTRQEILKKAELQADLNNAADLFEGLGVSNEHPRAKLLAEENDAMDSLMKKASFTKDTPIESHPLFKECESKKDYENLRKALGTAITSMEEKSSLNYASNLAIDLIRDISKPLAIESIRQTVATLNVLIKDKERQERQARLAKVRGGTATGGAGKKKAKSAKANLGGAFKKDSEIVMDSIDDYDDFNDDDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB12</accession>
    <name>A0A1E5RB12_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Kinetochore-associated protein NNF1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3084</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB12">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB12"/>
    <dbReference type="InParanoid" id="A0A1E5RB12"/>
    <dbReference type="OrthoDB" id="18453at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000444">
        <property type="term" value="C:MIS12/MIND type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007128">
        <property type="entry name" value="PMF1/Nnf1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03980">
        <property type="entry name" value="Nnf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0137">Centromere</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="155"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ84090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="224" mass="26149" checksum="670B6268B58F516C" modified="2017-01-18" version="1">MTTVYKHVRYAKLKEVCDQALDRVLKKLAXWSKLCVLYPSVSLRFQPELAKTQTEIIALLKTLCTNEFHAIFKERRIREKLNDLDELITIAKHREREFRAIENEAMEVDNEDTESNDKINASIDNQLKKDFPKIESFVPSDIVAFHKHSINLEFAEKLTQRLEQLKSVNADLEKEIETVHQSINEELDDLHTNIYKNNLGDMTSTETTTALRLQLIRQLQDLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB13</accession>
    <name>A0A1E5RB13_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytochrome b-c1 complex subunit 1, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3088</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB13">
        <property type="interactions" value="429"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB13"/>
    <dbReference type="InParanoid" id="A0A1E5RB13"/>
    <dbReference type="OrthoDB" id="10251424at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF126">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="1">
        <location>
            <begin position="33"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="2">
        <location>
            <begin position="175"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="442" mass="48475" checksum="C165FD71AD0AFE41" modified="2017-01-18" version="1">MLRASTQSLFKRTFTSQTSAKATVTKLSNGVTVATQPSTSPVSSVGVVFGSGASAENPYNNGVSQVLSNVFIKDDLKAVSKGLQLESFVGKEYQSFISTSSSIDASLEFLQSEILSNEISDSVFSSLKEQTLKATENYETXNHQGRVLQHLHAAAFQNTPLALPTVGTVESLINLEKSDLLDFASSQYVGSNAIVVGSGSNLKHDELVSKVEKLLKFSNGAKPELTKKSTFLGSEIRLRDDTLPKAWFSIAAEGVPLSSPYYYVSQVAAKVFGDYVATEPLSRLQGIKLIDQVNEYGIVDKYSHFSKSYKDSGLWGFSAETSNFXQIDDLVHFTLKQWNRLTISVTDLEVSRAKQSLKLQIASELNSNAKIINEXGSKSLASVESKSLDEIFEAIDAISVKDIKTFASEHLWDRDIAISGAGQIEXLFDYGRIRNEMSMMRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB91</accession>
    <name>A0A1E5RB91_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Proteasome assembly chaperone 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2794</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the PSMG2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB91">
        <property type="interactions" value="114"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB91"/>
    <dbReference type="InParanoid" id="A0A1E5RB91"/>
    <dbReference type="OrthoDB" id="10260712at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016562">
        <property type="entry name" value="Proteasome_assmbl_chp_2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12970">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12970:SF1">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010044">
        <property type="entry name" value="UCP010044"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00019186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ84167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="312" mass="35646" checksum="8148F78CCCEAC202" modified="2017-01-18" version="1">MGQTMDNQTQAKLILPLVSTGNVPQLAVDLILHNPKSNFKFVQDLKSQYLLYPFVGSLDTCKDLRNNAEHGKYPLYKNSDSKYYSSALELFHDSSSNTYIIQQRTPIITNFENNFFHELINKDLARYDIQEIVILDSMGPMELENIVSYTNINSQFFHENDRISVGHVDLSLEKLSKYFEDTNLLLSESSSSSNLSESPXPTEPVIDKTLLNFDSQCFQIGLNTTHQSFKLLYHILHQNSKTQIQYVNMVVYEGDNSHDAGLMVKNLFTNKQMQVKLPTNLIDWNNLVPPLSWDGVYGFNTFSGSMDEGLYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBB1</accession>
    <name>A0A1E5RBB1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2484</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBB1"/>
    <dbReference type="InParanoid" id="A0A1E5RBB1"/>
    <dbReference type="OrthoDB" id="2447880at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052145">
        <property type="entry name" value="Mediator/Homeobox_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24330">
        <property type="entry name" value="HOMEOBOX PROTEIN BARH-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24330:SF19">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="477"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="563"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="613"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="670"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="811"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="487"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="564"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="578"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="613"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="630"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="678"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="816"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="989" mass="109324" checksum="D32C4DED24E9D223" modified="2017-01-18" version="1">MNNNNIGQQIYFQKKEFSPNVLDNQENMTNGHYDNYPHCSLLKEKKLVYYISNKIFEFIGFKPQDCRLLDTMEIEEIFNILKGKKKVKNYMIPIITNNEVLINSNSLNETISMEESNSNNNINTNDNKIKDSSPGTTTHNSINNNRSGTPPPLINNNNIHNLVNIEDIVNMNICKNKKKKTSKVGKDNDKINDNANANDIANDNDNDDTDSNDYTQSKTLQIFKKTFIDCEVDPATPIYLDRLLSKTEYSNFLKVLHVCRYITYRCILRQFVYVKIPLELTTIVLNNGEKMYDDHSKSLSLENLSKKFSVTRWHLHRVFKSFTGITIKDFENIINLTICSNHHNQNVLKNYIMENKMFQDYNIFYIKFNDNDYWNDTNYLPPKNNSSTPNNITLEGYYIECVNDNPTKDLTIPNYSTSAYNINNSTFSSNQYLLQRLANFTLNDMNQYIAQYVPPQNQRNEEDVYMELEDFKKMLAKHKNSGNGNPASSASSSSSSSLSSSLLGXTKLNRVGKKTTKKKPQKSNKSSLSPKEENSTGNIAAISPVSLKEKTSSSQLLQXHDIVSNNNAGSSSNASSNDTFLTPPESTVPNTNNGMAATAQQYQLYNPAVQQQQQQQQQQQQQQQQQQQQETSGNPXDQRKSSFNQVVHDLYNADSSLFKTLEKQYFSQNQSMFTPGTSDPSQPEQQSQSRQGSLLQVPQPKSASHLQVPQPESAANLGGYFFQQHGNGYNGTNGVAGSGGFPSVFTPSFLTPGVAMGLGSNDGRSVSNLFGLNAQGATGNTPLGFSLTPSGSSFFARNLASPGFPIFSQSPNPIGSSNLNPSNNTTSNTNTSSHLSNVISASQSNGTPGSFNEKNSMISETIPNATSVSSATTINNSAPTLTGFDGGLSGMKLLRNDTPVQFFSSALADNGTSSLPPHMAQTSANGMGATSNVPGLETPGILNVPTPLLNPFNSLNSPFPMGIATPTIQALRKEQPSRAGSDLPIEFFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBC2</accession>
    <name>A0A1E5RBC2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Anaphase-promoting complex subunit CDC27</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3042</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the APC3/CDC27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBC2">
        <property type="interactions" value="921"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBC2"/>
    <dbReference type="InParanoid" id="A0A1E5RBC2"/>
    <dbReference type="OrthoDB" id="329563at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007091">
        <property type="term" value="P:metaphase/anaphase transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF13">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 27 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="782"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="816"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="850"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="952"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="499"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="22"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="198"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="505"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="524"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="538"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="5">
        <location>
            <position position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ84192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="996" mass="113205" checksum="3E5874D4A4E97239" modified="2017-01-18" version="1">MLFSSNSHQKAEPKRSLRNNVSSAPDPSSSPTALSSLPPEQESRXAQTFEPHHETFLQHNAKQRSEPVGSNANFLEDSLKLGEQDHLMPQPLFVTPSVKKTGFNDAVEAQNSEIRLDADDESVKRVNGFDLPKNRAIDKADKPRIMQGQDPTPLSKFLRHSSHLVNKHYERNASNEQKKPGLMNPMFINERRKREDSSNGNNANSGAGSHNGKHEFQSAYSSNANTSSNYHANESCGKSVYAKTGSSSSDSSSNAPESEYLQECENKFDYLYLLITNELQQGNYDTAEFIAEMFYSESMNLIXHVSHAEQTTSRVNTVLQKKLQLNKAYRGLYTYCYTLLLNRKYSTVVEISRKYLDIKINAPLSPPLFYSNGLAFMHAKSCLMLERNLRQGIKDLVDMEKLVWDNLEFVVNAEDANCIGYPNVATISCLLGKMFKKLDLIKQSAYYHCKALQNNPHLWESVKDLNSMGVEFNITNVFKEKLPKRQHKDXFNXEYDELNQKETSRXFNEQPTFSNLLNKKRPSDLNSDANNNFKKGKLKDFSKSVEPKKIGQNLPFVGEPLSSGSSKEHHPPNTPPKRMFSDEIPLSESIYTSPLLESNKSPRMFNAXVNVKTDYGKGAIQNGGFPGQALQIKKNGAAAKVKSNLPIQFSNSYGKNLAQMFKNEYCDNIMYLLFKSSKSGSDYNIYKAIRLLNSGDNACHSADVIPAFYAKYMPWCLKKLAVYQYENSDYKMSLKYFEQLRSIQKTSLETMDVYSTLLWHMKDKTQLSLLSHELLSLEPLSHITWCCIGNMFSVMGNHEDAIKSFQKSINLNKNFEYGFTLLGHEYMSTESYDLAKKCYRKAISLNPFHYNAYYGLGMCVFKIGEYEESLFYFNKAQSINRGNVVLITCCGLALEKLGYQEEALKYYEIATSLKPDSTLALFKKSYLLMELKFYTLALESFEHLKSMQPNDPTVHFLLGKLYKIMGKKKESFIEFTVAMNLDPKGSQLVKEAMESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBH2</accession>
    <name>A0A1E5RBH2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="15">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g2890</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="15"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBH2">
        <property type="interactions" value="1199"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBH2"/>
    <dbReference type="InParanoid" id="A0A1E5RBH2"/>
    <dbReference type="OrthoDB" id="270392at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02584">
        <property type="entry name" value="RNAP_II_Rpb1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02733">
        <property type="entry name" value="RNAP_II_RPB1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.30:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.390:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.274.100:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.140:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.180:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.120:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000684">
        <property type="entry name" value="RNA_pol_II_repeat_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007075">
        <property type="entry name" value="RNA_pol_Rpb1_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007073">
        <property type="entry name" value="RNA_pol_Rpb1_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038593">
        <property type="entry name" value="RNA_pol_Rpb1_7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006336">
        <property type="entry name" value="PRK08566.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF37">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04992">
        <property type="entry name" value="RNA_pol_Rpb1_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04990">
        <property type="entry name" value="RNA_pol_Rpb1_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05001">
        <property type="entry name" value="RNA_pol_Rpb1_R"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00115">
        <property type="entry name" value="RNA_POL_II_REPEAT"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 15 18" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="17">
        <location>
            <begin position="228"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1525"/>
            <end position="1712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1533"/>
            <end position="1701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1702"/>
            <end position="1712"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ84245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1712" mass="189913" checksum="87C3C9E8A9E958D0" modified="2017-01-18" version="1">MQQSYSSAPLRTIKEVQFGLFSPEEIRSISVLKISTADTFDEKGVPIINGLNSPLLGTVDRNNMCQTCHEGMSDCPGHFGHIELAKPVFNIAYLSKIKKILECVCVHCGKLLLDEHDLNMKQAMKIKDPKKRFNAVWTTCKTVNVCETDVLTSDNVYVSRGGCGHIQPQIRKNGIGFTVVYKKGKDADDDDEPEQKILTPDEVLNIFKHIISEDCWKLGFNEDFARPEWMILTVLPVPPPCVRPSVAFNESQRGEDDLTYKLGDILKANINVQKLELDGSPQHVIQDFEKLLQYHVATYMDNDIVGQPQALQKSGRPVKSIRARLKGKEGRIRSNLMGKRVDFSARTVISGDPNLELDQVGVPMSIAKTLTYPEVVTPYNIDRLTQLVRNGPNQHPGAKYVIRDNGARIDLRYSKRAGDIQLQYGWKVERHIVDNDFVLFNRQPSLHKMSMMSHRVKVMPYSTFRLNLSVTSPYNADFDGDEMNLHVPQSEETKAELSQLCAVPLQIVSPQSNKPCMGIVQDTLAGIRKMTLKSTFIDYAQVLNMLYWIPEWDGVVPPPCILKPKPLWSGKQILSIAIPKGIHLQRFDDGTTLLSPKDNGILVIDGQILFGVVDKKTVGSSAGGLIHVVTREKGPYVCAKLFSNIQKVVNFWFLHNGFSIGIGDTIADEKTMREITEAISVAKTKVEEVTKEAQANLLTAKHGMTIRESFEDNVVRFLNEARDKAGRSAEVNLKDLNNVKQMVSAGSKGSFINIAQMSACVGQQSVEGKRIPFGFADRTLPHFSKDDYSPESKGFVENSYLRGLTPQEFFFHAMGGREGLIDTAVKTAETGYIQRRLVKALEDIMVHYDGTTRNSLGNIIQFIYGEDGLDAGHVEKQSIDTIPGSDKAFEKRYRIDVLDKDAEIIKNLLESGSEVVGDFKVQTLLNEEYRQLIKDRRFLRKVFVDGESNWPLPVNIRRIIQNAQNTFKIDITKPTDLTIEEIINGIKKLEEKFIVLRGKGHILEEAQDNAITLFCCLLRSRLASKRIIFEYRLNRQTFKWVTDNIEAQFFKSVVHPGEMVGVLAAQSIGEPATQMTLNTFHFAGVASKKVTSGVPRLKEILNVAKNMKTPSLTVYLEPDYADDQEKAKFIRSVIEHTSLKSVTVASEIYYDPDPRTTVIEDDEEIIQLHFSLMDEETEAQLEYQSPWLLRLELDRAAMSDKDLTMGQIGEKIKETFKNDLFVIWSEDNAEKLIVRCRVVRDPKTMDADMEAEEDHMLKRIENNMLESITLRGVEDISRVVMMKYDRKMINPSTGDYHKVPEWVLETDGVNLFEVMSVPGVDASRIYTNSFIDIMNVLGIEAGRAALYKEVYNVIASDGSYVNYRHTSLLVDVMTSQGFLMSITRHGFNRSDTGALMRCSFEETVEILMEAGASAELDDCRGVSENIILGQMAPIGTGTFDVMIDDSTLAKYMPEQKSAFDVEGGATPYSSDAGLANTDIDIKDELMFSPVTEAGAADAMAGGFTAYGGGDFGDATSPYSGYGSTSPGFGGSSSPGFSPSSPSYSPSSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPQYSPTSPSYSPDSPSYSPGPHNDEHNNNNSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBH7</accession>
    <name>A0A1E5RBH7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Nicotinamide-nucleotide adenylyltransferase</fullName>
            <ecNumber evidence="16">2.7.7.1</ecNumber>
            <ecNumber evidence="16">2.7.7.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g2579</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate to form deamido-NAD(+) (NaAD). Key enzyme in both de novo and salvage pathways for NAD(+) biosynthesis. Predominantly acts in the salvage pathways via NMN.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 16">
            <text>beta-nicotinamide D-ribonucleotide + ATP + H(+) = diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21360"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="EC" id="2.7.7.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 16">
            <text>nicotinate beta-D-ribonucleotide + ATP + H(+) = deamido-NAD(+) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22860"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="EC" id="2.7.7.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 16">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; NAD(+) biosynthesis; deamido-NAD(+) from nicotinate D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the eukaryotic NMN adenylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.1" evidence="16"/>
    <dbReference type="EC" id="2.7.7.18" evidence="16"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBH7"/>
    <dbReference type="FunCoup" id="A0A1E5RBH7">
        <property type="interactions" value="512"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBH7"/>
    <dbReference type="InParanoid" id="A0A1E5RBH7"/>
    <dbReference type="OrthoDB" id="422187at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00332"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000309">
        <property type="term" value="F:nicotinamide-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004515">
        <property type="term" value="F:nicotinate-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd09286">
        <property type="entry name" value="NMNAT_Eukarya"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000074">
        <property type="entry name" value="Nicotinamide-nucleotide adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051182">
        <property type="entry name" value="Euk_NMN_adenylyltrnsfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005248">
        <property type="entry name" value="NadD/NMNAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045094">
        <property type="entry name" value="NMNAT_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00482">
        <property type="entry name" value="nicotinate (nicotinamide) nucleotide adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12039">
        <property type="entry name" value="NICOTINAMIDE MONONUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12039:SF0">
        <property type="entry name" value="NICOTINAMIDE-NUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 16" id="KW-0520">NAD</keyword>
    <keyword evidence="9 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 16 18" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6 16" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="17">
        <location>
            <begin position="107"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ83923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="338" mass="38527" checksum="FC2F6BF764239091" modified="2017-01-18" version="1">MDPSRAKNFIPPSDDNTPIGTLSPASAIAQSFTNVMMESVIADLNSSIDAPFEPAPAPMIKKDQIADLEEVPHGILRQAKKLDDYEFPVHRLKKKLTSENKIPLIIVACGSFSPITYLHLRMFEMAMDSINEETRFEVIGGYYSPVSDHYRKQGLAKSTHRVRMCELACERTSSWLMVDAWESLQHEYTRTAQVLDHFNEEINIKRGGVETPTGEKIGVKIMLLAGGDLIESFGEPNVWKDEDLHHILGNYGCLIVERTGSDVRSFLLSHDIMYEHRRNIMVVKQLIYNDISSTKIRLFIRRNMSVQYLLPNSVIRYIQEHRLYKGSEPVKQVMGSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBJ2</accession>
    <name>A0A1E5RBJ2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">PAB-dependent poly(A)-specific ribonuclease subunit PAN3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2460</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBJ2">
        <property type="interactions" value="661"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBJ2"/>
    <dbReference type="InParanoid" id="A0A1E5RBJ2"/>
    <dbReference type="OrthoDB" id="204958at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031251">
        <property type="term" value="C:PAN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030844">
        <property type="entry name" value="PAN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041332">
        <property type="entry name" value="Pan3_CK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12272">
        <property type="entry name" value="DEADENYLATION COMPLEX SUBUNIT PAN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12272:SF11">
        <property type="entry name" value="PAN2-PAN3 DEADENYLATION COMPLEX SUBUNIT PAN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18101">
        <property type="entry name" value="Pan3_CK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25586">
        <property type="entry name" value="zf-CCCH_PAN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="9">
        <location>
            <begin position="7"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="7">
        <location>
            <begin position="7"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="88"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="207"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ84262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="779" mass="86549" checksum="90F2EAC39255A96D" modified="2017-01-18" version="1">MSANADWAKDIPCRYITLYGFCKNEHNNCPYSHKTTSDLKFTDGASEFDPATRTEAEIATAAATATSSLSKLVPAANTSILDTSLLASAPKQQHQQQQXTHSTNERSATXVAASPKAKQMSKGESAINELKKAVSALKVGNTAGTTPTASTAASNSAASNTAAANSTAAKTSSKQFDPVNSPVFTPIYKPTSMSTNFLAASNSVPEQQQQQQQQQQQLAQQNSFNPYADNFVPAHGVQASFDQLDPRAFTNGGSMPTNSTPLFVSPSAPSTIGNPLQMSPGANQLSVLTGQTTQHPQQPQLPGQSNIPLTNQQPFHPAQHPMLNHLHQSAPMTPVGMMGSPSQGTLLTTAHDLSTSYVEHHLYNPDPPPHRKVPLEPHQRTPEMLFISNTLRIDLLDKKKDLANVQPHNVPGLPSVVNDYFNISFLYANDANNLYYKCVSNYDGVVCLIHRIANYTSENTPQPKKIASVYQACSKVHSSNVIQLKDIFLTTQFNDRSMCLVYKYHPLTISISDYYRFENQVGTSLGLEIDEEYVWLYLVQITNAMRKLHTSGLYFGNMLDFENFLITSIPGKIKFFGLGRNCLGNEPIRLKELQQHDYKKLGEMLETVITPKMRASNPDFQKILDYLLAETNLEVGEPKSVSEFVKIFAVKFFDIMENLQTQNEDVEQTLANELENARLFRLMCKMNAVINRPDSNSDINWSENGSKYPLKLFYHYVFHQKDHNGKKTLDLVHVLKTLNKLDAGVTEKILLVTPDEMNCMLISYKELKVLLEFSFKNIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBP6</accession>
    <name>A0A1E5RBP6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2958</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBP6">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBP6"/>
    <dbReference type="InParanoid" id="A0A1E5RBP6"/>
    <dbReference type="OrthoDB" id="4062897at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12120">
        <property type="entry name" value="POC1 chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038605">
        <property type="entry name" value="Pba1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018855">
        <property type="entry name" value="Psome_chaperone_1_fun"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10450">
        <property type="entry name" value="POC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ84317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="243" mass="27691" checksum="FCCA524935F9B6B1" modified="2017-01-18" version="1">MLFKQWNEQAEPRHILDDQFNETPSLVQVKTLPKFENVQKYFHEHHDKGSSIFIAPKRMHVLFVAYHAKPLHEFVCTYGSLEYKFPIYSSNSVSKSGSTNEGTKKTSVLIVDETFPIIVLNFFSNELSQYVNDNSTVVLGLSDRISXVKVIEPMKAPLEGKSFVPPEFVSNFGASLFHKAVLANYWFLPSEGPVGFEKVSQTNIGQLLQLIKDRFAVSDPSFXKRFAQNWNENDGTHVNTLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBS3</accession>
    <name>A0A1E5RBS3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 16">Serine/threonine-protein kinase Tel1</fullName>
            <ecNumber evidence="4 16">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">AWRI3579_g3036</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates histone H2A to form H2AS128ph (gamma-H2A) at sites of DNA damage, involved in the regulation of DNA damage response mechanism. Required for the control of telomere length and genome stability.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 16">Chromosome</location>
            <location evidence="2 16">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the PI3/PI4-kinase family. ATM subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4 16"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBS3">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBS3"/>
    <dbReference type="InParanoid" id="A0A1E5RBS3"/>
    <dbReference type="OrthoDB" id="3973143at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05171">
        <property type="entry name" value="PIKKc_ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038980">
        <property type="entry name" value="ATM_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044107">
        <property type="entry name" value="PIKKc_ATM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021668">
        <property type="entry name" value="TAN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37079">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37079:SF4">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11640">
        <property type="entry name" value="TAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01342">
        <property type="entry name" value="TAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="16" id="KW-0158">Chromosome</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 16" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10 16 21" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="12 16" id="KW-0779">Telomere</keyword>
    <keyword evidence="7 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAT" evidence="19">
        <location>
            <begin position="2028"/>
            <end position="2351"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="18">
        <location>
            <begin position="2456"/>
            <end position="2764"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="20">
        <location>
            <begin position="2785"/>
            <end position="2817"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="2188"/>
            <end position="2243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU365027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OEJ84339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="2817" mass="322918" checksum="DE92015AD9A80D47" modified="2017-01-18" version="1">MGLDEDTLSSILDFLEKSTVRERTKGAEKLKSVLNENPDDIPVRYFQDFLKTLIDFFEYENVKRQKLLKKLTDIDGNVEESKTLKSMDEKTIEAAFLARLFVEKTSHRFTGKHLNFFLSVLLNIVHSEEITFSGRPVSNXSGSSASNISKHEIKHLPLGVLTNISFAVARLVKNPTFHLKKSNELWSQTLETGLYSLNALMNDGITVAPDVQNWLNVLNELLKQDTIDIKSFSKGIVNTVYRFVSSVTRETSNCAKTIELVNKTLLRTHLSNINCLKLIKPTVTLISKTQYLRNKDLCLEVAYFNVFISKLLPFSKSLSNTSSMFVDWYLELTNHLKTTLNLFQEKKFDFKVQFKPLGCFNTTXKWTSFADFQLADIQAMSGVDYASKWLLLYSVVRLLEVYYARHEQELLQKSNSVITTRQTELGIFSEDKVATKKPKYTYDLLTECFSNSASMSEFIMNCLIEGSMEENRVFGLQVFAFCCARHDLDFDHSMLLKRVIPLMGNDLFYQWCCLAIITLVSFEQYEMSTNDFENILKLTLPKLAKTKNFDITCVTITRLVTFQPKQLQDNTLLYRIYDTIDAFVINGPVNVTEYSLDLWKSLYYLAPAYKSSTRETSIDKFYQWLDYKWLSFVRSASIDTLIGLSNFVLWLCNKKESVPQISFNEERFETLYYSDWEYLDAERAFLYWPINLPNKKFRKTVQISYDGLNKTVVRLVNEKLNDSLSDIQDIPEFLQKAALWVGSLHLVNSLSSDSRYLPEVASLTDXLAMALKHIKFESYEHLILFVTMLRRIDLSALPQSLAFELRRLNFKKVYLTSIKXAASVQEGDFLDNFTQPKRGALTVTDVTTSCSTHVTHLLERQFYTEDVEKILFVEVTLTALLDNTSESLMSCLDFLSSLDTEIIFAAGKFFLAFFEKGNIQDCDEGVMKILKGMAELLLTTAYNTSNQSIETLSELLIVTMPVWLELGNNEYMEDARTIFSWLEKCFDKVAFNGSFATFSFYKLVIKLLQKSKXLKPLQVPKNALFEQLLLCLSSMPIYFSSKCMKDLGKYMLSMNESSKNHFVTKMSQSLNVDTLGFSNKAMFLYCITEFSSVSQTNLLKFINSITSYTHDVSLCSYVEKSLNLLTSSFGFTSTMQILEMCKFTVLEKWYLDAVQNLDSDHLPLDIKCFGVQSLEMFFTQFQIEIIAFHFAHQIQSALIMDTIQKVTSKKAVELVRASIPLIFALAYSDIGSGSETIRSAEKVVGSGFLFSNKIPIAYSILTFCACGDLSYTRNQLAECFPNSFLKDVIMAGNHLKFYNGLNIQILHIKVIFSKHKIFSSFTQXEVNLLILKIISNLXKVSTEEEEIQTIRILKMALILFEDLIHGDAFIQYVETQVCRLMQKQSTHDEVAYLLVGLCEIHNVSEKTTGAFFSAVFESLLNYRRTNSSEINKPLLDALEVSLQNCEQVHVFWNTALQYLSYQXYSASYTHDVSTLFACDEHDAVFHXNFLSALFAIFPEPVFDEVSGNLSILVVDALRGVEVPLPSKNFLLWKTFYLSSHFEQTLQISMPKIXHAKHKEMLDSFGXLTQLFPLICRNVSNTTAAAVRFASECALGAFYGKPLEEXSLFQLSKVDYKAWKQDILPIDVDSFFMMNKPCDIECGLQGFSTSYFRDPAISYKKWLTLFWCSFVSELAHIDMVLVFASLATVDFCFVEDSFVDFFLFSLYFSNTKGVFTNILSSIDVLTECKESEKKISKLLEIIEVIRAASYYSSSKEQTKLVQKHLLEVYKSLPKLLLFETSLEIQNLHFAFLILDETYQSFQDYEGNIQDIQKLFLNFQDEDLVSCIPKAPSLLTSLELLNFSHAQGLKRFIFNSSAIDTGYLNMGGESFYNSFVESSVQNRFSSVSKLFLDNSANAAYYDWYDNLDDWDLPDVGTYQTRAQIMHAFIQGFRNGQSSDENNVQQIVSKFSTSGLGNTFLIDTIPDLLNMPKLLDFDSSSYASFSNFLLKKDKFMLESIGWSNYEVSLRARIKFLSYSYLSNMKNQENIGLAKAAELASYNKRCLQFKDGQLALKSAMELDQFVSTEMRNHSKFIDLKLLSDVCSAATLWYQGESIAPVQILKRVLSVSGANXQNFEKVRPSESYISSLLVEYLSXSRQMLPMKIYQSYVLPYKANLELLSSFEERSKIYYTLGEFFYKHAKTLLTNSDLKFKQKQFRKYQQELKDLELLYYNSKSQSEKKDASKYFKIMQDQLAQEKQALKDYDXEKRLFVYHCLEHFAKAMKFSSSARSDILDKFCALWFQVSDEENINEQMLGIISEVETFKLLPWINQMVSKLSGTQTMFQRTLSRTIKRIVFNFPYESIYHLLNAKLYGNSRSEGFESRLQAIDSILTSLSRKGDFEKRYLIPITDFVKNCVELACKNLEPNVKFFDLTTHTVGSFWTSILPEYDLPLPTVCVSIISSDDGXKKERPRILRVMPKVNISPSGISRPKIMEFLLSDGSKKKILIKGNDDLRQDAIMQQVFKQVNLIFSSHQHSRKKKLRVRTYEVIPMGNKAGIIEFIENASSLRDVLVPLHKRDGCSLSAARETMKRMLDKSKEEKVAVYTKMTSQIKPVFRSYFFQRYTNPEKWLETRYXYTKSLVTTSVVGHILGIGDRHLQNIMVDEESAELVHIDFGIAFDRGKTLTIPELVPFRLTRDMVDGLGIAGVEGLFRKNFEYAYKLLREEQEKVMSVLNILRWDPLYSWSISPLKKARLQALRDADNQQATDMAAKGSEKVKSSKVIAEEVDEETNDKSVIALSGVEKKLXGLSVEATVQELITEATSVENLGVIFYGWSPFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RCC5</accession>
    <name>A0A1E5RCC5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein SCM3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2892</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RCC5">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RCC5"/>
    <dbReference type="InParanoid" id="A0A1E5RCC5"/>
    <dbReference type="OrthoDB" id="2420608at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018465">
        <property type="entry name" value="Scm3/HJURP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10384">
        <property type="entry name" value="Scm3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="272"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="301"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="340" mass="38770" checksum="76E06779571050B3" modified="2017-01-18" version="1">MNRHTNKLGKKXRKKINLKKLNNAVKGLLEQEKQSKETPKFSKTKVLPINTNSDVKDETDSNNSSSIPKEPSPIVTKIKSRTLPWSRKDLIDLDPESGSDVEIDEKSPLSDQDAELHQEAKETVNKHGVIYTMSKENKLIPKFTDDEIMEKHKQADANMKETWMNIINKYSFNEEDEEYGGVKSDVIDIRTGQIVEDNGHLRSLYKKNKSNGYLDDKNTVRNDKYVSKLLEEYEFDLEKSAKHSSRKHLSKEKVSRDKEIGKQGSHVSQYGSDSSDSSDSSDSSDSDYIDESXVDDSDIWGDEKKDYGFQDKTHLEVGKTTKNGVNKESEYSDSDTDFTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDU7</accession>
    <name>A0A1E5RDU7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Polyubiquitin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g2317</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the C-terminal section; belongs to the eukaryotic ribosomal protein eS31 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the ubiquitin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDU7"/>
    <dbReference type="STRING" id="56408.A0A1E5RDU7"/>
    <dbReference type="InParanoid" id="A0A1E5RDU7"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031386">
        <property type="term" value="F:protein tag activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000004">
        <property type="entry name" value="Polyubiquitin Ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000008">
        <property type="entry name" value="Ubiquitin-40S ribosomal protein S27a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="14">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="14">
        <location>
            <begin position="77"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ85046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="155" mass="17352" checksum="FEB0A8806CF7197E" modified="2017-01-18" version="1">MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGANA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDW0</accession>
    <name>A0A1E5RDW0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Increased sodium tolerance protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2143</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDW0">
        <property type="interactions" value="306"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDW0"/>
    <dbReference type="InParanoid" id="A0A1E5RDW0"/>
    <dbReference type="OrthoDB" id="3973153at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF73">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="226"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="369"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="447"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="520"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="7">
        <location>
            <begin position="133"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="644"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="671"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="687"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="767"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="778"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="828"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="935"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="8">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ85087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="950" mass="106387" checksum="875C67C5B3810B4F" modified="2017-01-18" version="1">MSLNNDILVTKLNPNFFIATGDKVSEEQLNKFIFLINSELDPNAELTTRPGVSLGSELILVKITLSEREDPKRKLEKLVNNFDKSFQVFSXESEFQKKNKHKFSSKLLKKFPLVNSLTKADLTKLGELTTPDVALHFAYAQTYTKALVPLAFCGAVMRFTFSSWEFNYSYTAYLFLWCFVFINYWKHFKESSLQYLINSNKDAVSAFDYEVEAIKEKSGFLFIKKLMFVPVAWTFMICLLSFQLLCFIVEIFLSQLYNGPLKSLLTLTPIVLGTVFIPMITGVYSTYVDKMVKWENSPITAHRSTIEKKFTLTFMTSYVPLFITLFVYLPSGYLLNPYLNSIREXCSKYHVSVEKGAFHVDIDRFRGQFYYFTVTGQLIAIFMEYVVPVLMNIFKKTYKFKDLKKINEADNSQESSMLSSLRESVFNSWGSFDPVESYGKYIIQFGFIAMFSTIWPLAPLVAILFNIIIFKVDLWKALTRATIVKPEEEHGMPXGKNKQTSISTLSRNPAFLTIYPWNKILTFLAWCATLVSPSLILMYKFSFFPGVGYDFDTSLEKRSLEYLEAPIGLDWKTIVAFVAIIEHFGFMTNYIFGKLATYKAEKLLKKSKLHEYXEQAQVNEKHALSQAPSQQEKETYHIEKNDINMNTSKSGKHANESSINEKQTVPKIVTSSEPDAAAPGPVYAQIPKTSGQESAXVNAQKNVQDPPTTAFVPPKAPSKAENSTVASKAPSKAENSTVASKAPNRQGGETDEELSVAGATLPKIIPTSENFDSRQNLSDTDSVPSVDTTTPSSAPDSATAMTPASSEASKAKAGPMPAHASGPAIVPQTASPNVSQPRIQPSSVKQTEATPSTQTNPSPHXVKKQAQVPAQSFIKPEYDPTNPTLAPSAAAAALHEVPTNIENSXHDQRPQRHHSSRXPSSNHRNDVSSHSSXERKKKSKNIFTKIKNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE01</accession>
    <name>A0A1E5RE01_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sulfhydryl oxidase</fullName>
            <ecNumber evidence="8">1.8.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2364</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2</text>
            <dbReference type="Rhea" id="RHEA:17357"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16520"/>
            <dbReference type="ChEBI" id="CHEBI:17412"/>
            <dbReference type="EC" id="1.8.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.3.2" evidence="8"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RE01"/>
    <dbReference type="FunCoup" id="A0A1E5RE01">
        <property type="interactions" value="454"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE01"/>
    <dbReference type="InParanoid" id="A0A1E5RE01"/>
    <dbReference type="OrthoDB" id="17199at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016971">
        <property type="term" value="F:flavin-dependent sulfhydryl oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.310:FF:000003">
        <property type="entry name" value="Sulfhydryl oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.310">
        <property type="entry name" value="ERV/ALR sulfhydryl oxidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039799">
        <property type="entry name" value="ALR/ERV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036774">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017905">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645">
        <property type="entry name" value="ALR/ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645:SF0">
        <property type="entry name" value="FAD-LINKED SULFHYDRYL OXIDASE ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04777">
        <property type="entry name" value="Evr1_Alr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69000">
        <property type="entry name" value="FAD-dependent thiol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51324">
        <property type="entry name" value="ERV_ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 8" id="KW-0274">FAD</keyword>
    <keyword evidence="3 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERV/ALR sulfhydryl oxidase" evidence="9">
        <location>
            <begin position="75"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU371123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="180" mass="20647" checksum="39B38EC0E65F2C9A" modified="2017-01-18" version="1">MSSENTHDKNFVKNNQIGVTGRKIIYDEDGKPCRSCNTLLDFQMVTGKISSTATPGHTSKSLAQSNQTLLFNKQDPPDVEELGKSTWTFLHSVAAKYPQTPNKEQQGDMKNFINIFSKIYPCTWCADDFKDYLEQNAPKVGSRDELGKWFCEAHNEVNVKLGKEKFDCNLWKKRWKDGWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE45</accession>
    <name>A0A1E5RE45_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Coatomer subunit beta'</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g1877</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasmic vesicle</location>
            <location evidence="1 13">COPI-coated vesicle membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Golgi apparatus membrane</location>
            <topology evidence="13">Peripheral membrane protein</topology>
            <orientation evidence="13">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="13">The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the WD repeat COPB2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RE45"/>
    <dbReference type="FunCoup" id="A0A1E5RE45">
        <property type="interactions" value="1353"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE45"/>
    <dbReference type="InParanoid" id="A0A1E5RE45"/>
    <dbReference type="OrthoDB" id="10261470at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22947">
        <property type="entry name" value="Coatomer_WDAD_beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000016">
        <property type="entry name" value="Coatomer alpha subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000001">
        <property type="entry name" value="Coatomer subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006692">
        <property type="entry name" value="Beta-prop_COPA/B_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050844">
        <property type="entry name" value="Coatomer_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016453">
        <property type="entry name" value="COPB2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011048">
        <property type="entry name" value="Haem_d1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056176">
        <property type="entry name" value="TPR_COPA_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876">
        <property type="entry name" value="COATOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876:SF2">
        <property type="entry name" value="COATOMER SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04053">
        <property type="entry name" value="B-prop_COPA_B_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23953">
        <property type="entry name" value="TPR_COPA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005567">
        <property type="entry name" value="Coatomer_beta'_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51004">
        <property type="entry name" value="C-terminal (heme d1) domain of cytochrome cd1-nitrite reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 13" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <keyword evidence="5 14" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="9"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="93"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="136"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="180"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="224"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B second beta-propeller" evidence="16">
        <location>
            <begin position="342"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B TPR" evidence="17">
        <location>
            <begin position="615"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="834"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="834"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="851"/>
            <end position="869"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR005567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF23953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ85165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="881" mass="99330" checksum="B361D52A1DCB563D" modified="2017-01-18" version="1">MKLNIKKAFSSRSDRVKGIDFHPTQPWLLSTLYSGKAEIWNYETQQLVRSINITDVPVRAGRFIARQNWIVVGCDDFKIKVYNHNTGEKVADFEAHPDYIRCVAVHPTLPYILTSSDDSVIKLWNWEKNWALEQTFEGHEHFVMWVEFNPKDPNTFASACLDRTVKIWSLGNAEPNFSINTHETEGVNYVNYYPQQDKPYLLTCSDDRTIKVWDYQTKSCVATLEGHLTNVSYAIFHPTLPIIISGSEDSTLKIWNSTTYKLEKTLNLGLERSWCISAHPAGKRNYVAAGCDDGFSLLSMGNDDPIFSMDPVGKLVWCGGKVGTSASDVFTSLIKPVDEEQAETLEDGSLVPLQVKDLGNVDVYPTAIKHSPNGRNVAVLGDGEYIIYTSLAWRNKAFGKCQDFAWAPDSNSYVILDQQGVLQLFNNFKAIKVLDVEITDSIEKLFTGPFLGVKTVDGFTSLFDWESGSLVRRIDVDADQIVWSSNEELILISTQTSNETEAENTSGYVLEFNKEMYDESLSNGNVSEEGVEDCLDVLYEFNDVVRSGKWVGDVFIYTSQSDKLNYFVGGKSYTLAHFDKPSYVIGYLPKDSRIYVTDRDMTVTSYSISVAVLEFQTLVLRGEIDAAFEEILPNVSGKDNLMKISRFLEAQELYQEAFDVAPDADQKFFLALKLGYLEQAFELLSKDESDSKWRTLGDAALKKFNFKLAIEGYSKAHDLESLFLIYSSFNMKEKLIELGKLAEEDGKFNVSFNAYWSAGDISSIQSLLASVGRVPEAAIFGYTYGSDVEGFVEAWKQKLNAEGKQKLADRIILPHIKKTENLIDFEQAGVATEAVEEQQVQQTADNHKEEEVEEVEAEEEEAKPEEQQEESNIAENQDDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE84</accession>
    <name>A0A1E5RE84_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein MAK16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2233</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the MAK16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE84">
        <property type="interactions" value="1010"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE84"/>
    <dbReference type="InParanoid" id="A0A1E5RE84"/>
    <dbReference type="OrthoDB" id="10251342at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.110:FF:000001">
        <property type="entry name" value="Protein MAK16 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006958">
        <property type="entry name" value="Mak16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029004">
        <property type="entry name" value="Ribosomal_eL28/Mak16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23405">
        <property type="entry name" value="MAINTENANCE OF KILLER 16 MAK16 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23405:SF4">
        <property type="entry name" value="PROTEIN MAK16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04874">
        <property type="entry name" value="Mak16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01778">
        <property type="entry name" value="Ribosomal_L28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003352">
        <property type="entry name" value="MAK16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal eL28/Mak16" evidence="6">
        <location>
            <begin position="5"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="192"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="194"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="253"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="272"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR003352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="305" mass="35638" checksum="7B3FAB786DE19FC0" modified="2017-01-18" version="1">MSDEVIWQVINQNFCSYKLKTDKNQNFCRNEYNVTGFCNRQSCPLANAKYATVKNIDGRLYLYMKTAERAHTPAKLWERIKLSKNYQKALKQVDDHLLFWNKFLIHKCKQRLTKLTQVAITERRLALREEERHYVGVAPKVKRREQNRERKALVAAKIEKAIEKELLDRLKSGAYGDKPLNVDEKIWKKVMGQXEDEQELEEEDEEDWDDEEDEESDVGEVEYVEDEDDELVDMEDLEKWLGDESDSAASGSDDSEEDSEDDSDAESEAGAAKKRSKKASQGKKRPRVEIEYEQETEPQQQEMAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REI6</accession>
    <name>A0A1E5REI6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1919</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REI6">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REI6"/>
    <dbReference type="InParanoid" id="A0A1E5REI6"/>
    <dbReference type="OrthoDB" id="10261782at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038921">
        <property type="entry name" value="YOR389W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35204:SF1">
        <property type="entry name" value="ENTEROTOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35204">
        <property type="entry name" value="YALI0A21131P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="180"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="654" mass="73838" checksum="F316D85386F5B877" modified="2017-01-18" version="1">MKAIVRNTILGVIIIANLSYFAYVYLVDKSDYDFARALFLSTPENVSKPSYEDVINATNFQKHLRPLDLLNASALFNSVNSALKQKDSDIFPSGVSIFPASIPQGTLLYHAGTGFPDSFEWLAMDVEFSIDFGARGASTRGRKSLQNNRRHGPGGPGGRMPGKSDETVNTDMEPIDYCKPNSSPSFGGPGRSRNGIPMTLMSFQAKKNLDKLIYFDGASAAKTVETGEMDTQVLLSDEITKRMHINTTDDAKEHMAERIYADRICRWGKQFGLQGFVRLELGFELVICDFHENLDMVSNVSVAFADKMYNLPAPVEITASNGWPINCTDGSLIEDQLSTEQNETLALEDFRQEKLSKLEVLLPWEHARIGGDSHNRGDKRIGIDYRHLVTGFNRTYLDPSAYKRKLLNAPNATLVEMFDDLEDVVXHQSAFHPELSNNWQLRTEEIETKFTVFFIALEKILSNSKEVSLQERVLSASKYTWGFVKRFWSKVDEDYHTGKNFAXWEYSYPVFKLTTESDFLIWSSLAKVVXSCVDTIYEAHDVLSPIAEEFLLSGNITELDSKDAQVAQLAKKITQFKDTLQWVSFGYECSKKCAWDEICYTPSWGPGPLGWMAPTSKVREGEQLPAGFKHDKNIDMIVIDTEQTCFKMDDLLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REN4</accession>
    <name>A0A1E5REN4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutathione peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2351</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the glutathione peroxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REN4"/>
    <dbReference type="STRING" id="56408.A0A1E5REN4"/>
    <dbReference type="InParanoid" id="A0A1E5REN4"/>
    <dbReference type="OrthoDB" id="446890at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047066">
        <property type="term" value="F:phospholipid-hydroperoxide glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140824">
        <property type="term" value="F:thioredoxin-dependent peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00340">
        <property type="entry name" value="GSH_Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000010">
        <property type="entry name" value="Glutathione peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000889">
        <property type="entry name" value="Glutathione_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029759">
        <property type="entry name" value="GPX_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029760">
        <property type="entry name" value="GPX_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592:SF78">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00255">
        <property type="entry name" value="GSHPx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000303">
        <property type="entry name" value="Glutathion_perox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01011">
        <property type="entry name" value="GLUTPROXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00460">
        <property type="entry name" value="GLUTATHIONE_PEROXID_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00763">
        <property type="entry name" value="GLUTATHIONE_PEROXID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51355">
        <property type="entry name" value="GLUTATHIONE_PEROXID_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 8" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="9">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000303-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="162" mass="18252" checksum="C287D8EFA5975151" modified="2017-01-18" version="1">MSKFYALKAQTKSGADFPFSDLKGKVVIIVNTATKCGLTQSGLSGLEELYKKYHADGLEIIGFPCNQFLNQNPETDEEGENFCKLNYGVTFPFMKKCEVNGPNTHPVFDYLKNEKPGIFGLTRIKWNFTKFLIDKEGNVVERYSPSTNPAKLEPKIKELLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF14</accession>
    <name>A0A1E5RF14_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2409</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF14"/>
    <dbReference type="InParanoid" id="A0A1E5RF14"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ85508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="166" mass="18554" checksum="2968B3DF51EC08A4" modified="2017-01-18" version="1">MSEFQHISNNGLDSGEKVNHHRVPEFCLTSIEQIITEIRYVIKSNDYLQKNVATSSTVKNGPESLVWLDEVVTSLIEHSVHPEIIDSWYKGDKSALDQALRSKYENEGLNEHQLNKVESLLSEKLANVIKTDLGASVFLTSSGASQEQIASIQSAKDLKEWLSLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF29</accession>
    <name>A0A1E5RF29_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF29"/>
    <dbReference type="InParanoid" id="A0A1E5RF29"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="67" mass="7660" checksum="841ED0F45AC2410C" modified="2017-01-18" version="1">MSDHKASSTAGNSEVTAENPPKLNTFYDIINNLQKSLDEIVYTNKKSDEEMRSRIAAMEKRLKELEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFA0</accession>
    <name>A0A1E5RFA0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein SPT21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1863</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RFA0"/>
    <dbReference type="OrthoDB" id="3973237at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000183">
        <property type="term" value="P:rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042403">
        <property type="entry name" value="Spt21/Ams2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39147">
        <property type="entry name" value="PROTEIN SPT21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39147:SF1">
        <property type="entry name" value="PROTEIN SPT21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="408"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="454"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="579"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="741"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="822"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="408"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="746"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="889"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="2">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="906" mass="100308" checksum="F8A14880E4112963" modified="2017-01-18" version="1">MSETRTRIEKLILKVLYTVDNESSAYLSRSTFPVPVKIVEFTPEMQQSNAQLPSSLGVAYLYDALNQVYNNSPELFANELDANIFDKNEVNISDKNEVNISDMNEANIKNKSNINGTNANEMNSLKTSFQDCNVKYKSHKGQKSECSRSMNLPDFNVYYKDITEDNEPFVSLGLLSNLQSVKTPIDDPVANSTTKYYNEIFQDPNCPLVFGRICTNFSSFIRANGNSSGNAYTNDILEIKLRFSRAQNSMGKSHMSNYGNNNTSLPTNYIIPSVKASFTTRRNSNNTVKPVPVRKTKRLTNPKPAPKATKTQSLPIWNNSMVNPASTGSNXNGSSGIPQRRILPASSIIKKIQQGDQRNMGEMGGEAQSSPSYXESVMMTTYNXTADSTPIAVDVSKRFEFASKLKSSKNNNNVXNXNSNNNAVRLHGNSSSSPLGNVSILXKKKPXVKDASLTNKKKKKKXIKNAQIRPSLNAQPNVDFNSTXFFPPANTHVDNFSKIMMLEKEDEEDDEDDVDIRTNVIDNPESKDHYSPTHGNTASNXDAKMVNMVLDGLPILEEEGHEDILDMDMDLLKHHTTAAVENTTGKKQSKKRSLANVPATNKKKQKSNNMEPVLSIHKLVAMDKENIIPPSAMDSNQPLTTTTGLVLPDITIDDFFSAHVYPTIVEEDESKNLIPNTNTLTHPNHQEVLDIALDDEEEEDEEEDEEDGTGNDATTKMQQEQWFNDFLHAEYASGIAVPEYEHATNDNDNTPKIPTPSTTTNQAPRHDEAKDQQPGGPADLYSSLSSSSLSXDYMDDYTSTTSTTQTDALVXENCCSNTPLAPSTSXPIKAPGSSKKFNKPLDGYAFPTRISNNKHTRIMPSSPPTASNKNDDNSDVEEXFQRNRGSLMESASSTPGNLNTAEVIQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFD9</accession>
    <name>A0A1E5RFD9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoserine transaminase</fullName>
            <ecNumber evidence="4">2.6.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g1985</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>4-(phosphooxy)-L-threonine + 2-oxoglutarate = (R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:16573"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58452"/>
            <dbReference type="ChEBI" id="CHEBI:58538"/>
            <dbReference type="EC" id="2.6.1.52"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-serine + 2-oxoglutarate = 3-phosphooxypyruvate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:14329"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57524"/>
            <dbReference type="EC" id="2.6.1.52"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.52" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFD9">
        <property type="interactions" value="575"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFD9"/>
    <dbReference type="InParanoid" id="A0A1E5RFD9"/>
    <dbReference type="OrthoDB" id="1703350at2759"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00197"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004648">
        <property type="term" value="F:O-phospho-L-serine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000010">
        <property type="entry name" value="Phosphoserine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000006">
        <property type="entry name" value="Phosphoserine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00160">
        <property type="entry name" value="SerC_aminotrans_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022278">
        <property type="entry name" value="Pser_aminoTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003764">
        <property type="entry name" value="PRK05355.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43247">
        <property type="entry name" value="PHOSPHOSERINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43247:SF1">
        <property type="entry name" value="PHOSPHOSERINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000525">
        <property type="entry name" value="SerC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 14" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0718">Serine biosynthesis</keyword>
    <keyword evidence="7 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="13">
        <location>
            <begin position="13"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ85576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="395" mass="43977" checksum="7AA2AF4609EFB8A7" modified="2017-01-18" version="1">MTALPTREEPSYFGAGPAVLPTHVLEQAAKDLINYDNLGLGVGEISHRSKEATQIINDTKENLIKLMDIPKDTHEVFFMQGGGTTGFSSIASNMFFDSYDASKKDTEQKKGLYLIDGSWSLKSFQEAQRLGYSVESXKLDTNNTEKGASILNEIQYPENLSYVYICENETVHGIEFPMLPANIPQNVELVADLSSDILSRKIDVSKYGVIMAGAQKNIGLAGLTVYIVKKTILNNIAKNEKNLDNASKYPKTPIAFDWPTVVKNNSAYNTIPIFTLHIMNLVFKNLLAKGGVAQQELENQKKSKMLYQTLDKYPNIFNLPVSKNFRSKMNVVFTLKDSSLDDEFLSNAEKQNLKGLKGHRSVGGFRASLYNAVSLKAVEKLVKYIEEFASTVESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFF5</accession>
    <name>A0A1E5RFF5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mediator of RNA polymerase II transcription subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Mediator complex subunit 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2181</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the Mediator complex subunit 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFF5">
        <property type="interactions" value="203"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFF5"/>
    <dbReference type="InParanoid" id="A0A1E5RFF5"/>
    <dbReference type="OrthoDB" id="5310959at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019680">
        <property type="entry name" value="Mediator_Med1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10744">
        <property type="entry name" value="Med1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0010">Activator</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit Med1" evidence="9">
        <location>
            <begin position="13"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="136"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="151"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="171"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="519" mass="58835" checksum="9438A227187D39B9" modified="2017-01-18" version="1">MSSKDDFKNQLSEMIDLLQVYRPGKISKENVECLCQILGLETFIDNLSANAGTRLSIASKIIVIDIDFDNSSERSVKDAKLVLASNFDNFNYYNAENKTNILFNSLKSSQYSDLSIFTKNLKFLRLLDAYSNTSLDXDSDNNSGNGELMGKTQSGGTSPQNSLHTGRYSVSGGNTNGRISNSMNNSPADRSPFSKMRGNKQSSSFDLFRYYTNLEDYLSAFLSDFEPYLKITSNVNDIFGIYFMNKRTETVIMKLCIKEKTPSDNTPGVLQEFQYQNNKWICENPNKMIESLYLALEIIDPDLLFMEHLISSDLVLDPLPCSTYPAPTTSIDNVATSGSSTIQMTFTSTFHHYHTFNVSNENLEYLKDISKWALWFQNVLAPSFNKCVKQIKLQKAQHKMNXSGLHRLSGENRRPSLSIMKEEGIGELNMHELVKKNTPDEDVFMDDELPVAESLPYTACSDSLKLQSTQTNEKQGGKNSKEMIILSEDYVYDEYKGSVTFYDDDFSRWSEFSEESINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFI3</accession>
    <name>A0A1E5RFI3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Kinetochore-associated protein MTW1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2212</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
            <location evidence="1">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mis12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFI3">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RFI3"/>
    <dbReference type="OrthoDB" id="1884855at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000444">
        <property type="term" value="C:MIS12/MIND type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051382">
        <property type="term" value="P:kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008685">
        <property type="entry name" value="Centromere_Mis12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14527">
        <property type="entry name" value="PROTEIN MIS12 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14527:SF2">
        <property type="entry name" value="PROTEIN MIS12 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05859">
        <property type="entry name" value="Mis12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0137">Centromere</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="288" mass="32829" checksum="A68752BB56CA0987" modified="2017-01-18" version="1">MSAHTLENASILTEHFGFPPISLVDDIINSVNMLMYKCTEAMEDYLVKTVDNTYDNNGIDISEREAYLNEIKEGTSKLESTLETAVDIHFDKLELYLLRNVLNVPHLDTRFFRLRHQLGLRKIEANTEEKLQLQISELCTSISXEVQYNKMLQEKLASFKKLELKIKNFKRAVSNFFALSQKSAAFPGDLPFALESSLTNALGPIDKSLQQVLQFLNEFEVSKADMHGKQNQEINQIHAKLTEMLDPNKDAVVNTGSRLQYLNTNYFREVAENDKVLDGDSSFXLGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFM4</accession>
    <name>A0A1E5RFM4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein HIT1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2194</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFM4">
        <property type="interactions" value="95"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFM4"/>
    <dbReference type="InParanoid" id="A0A1E5RFM4"/>
    <dbReference type="OrthoDB" id="18412at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23024">
        <property type="entry name" value="zf-HIT_ZNHIT2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040722">
        <property type="entry name" value="Hit1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007529">
        <property type="entry name" value="Znf_HIT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18268">
        <property type="entry name" value="Hit1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04438">
        <property type="entry name" value="zf-HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51083">
        <property type="entry name" value="ZF_HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HIT-type" evidence="3">
        <location>
            <begin position="3"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="13"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="162" mass="18609" checksum="ACF70BB3A243937B" modified="2017-01-18" version="1">MNCEVCQEEPSKYKCPICQLKYGSLACYKTHQKTHANEATXPEPEKQCEKKTTALKDTKMEDQSEMESSSNQLIHEKLHTSIYQNLLDHNKIIQELLSYNAVKYHLHKVYKILTIPEGASNTVQSTEQKEQMAVDYLNTLRTGGNHYNEAVEEFCQVVLSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFN0</accession>
    <name>A0A1E5RFN0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA repair protein RAD7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2202</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFN0">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFN0"/>
    <dbReference type="InParanoid" id="A0A1E5RFN0"/>
    <dbReference type="OrthoDB" id="1924287at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF190">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="619" mass="69935" checksum="18894228C6CB6DBE" modified="2017-01-18" version="1">MYRPLRKRSKKNGAETPSTGEADTGVKGPNSALTAFLKEQGINANTIRDKWLKSQKEKENEEESTPDQPDEIVISKDGTQEKGPIITEKSQEPVDLGSSEDDLSVESTPVLVSNRRLRSKRGLDTSDTETNDKNVXEDDXSNELARSNRRRRIQQESDSDEEEFVPSVSRKELNSLXTETSGSPAPKAAKTQQQKRVRKKKAADLLDRRRSDKIRSLQELSIMKISEQITKQHGPDDIQSKSANQSYSRLRDVLGGISTQNFQQLSKTLCKNRALNDDTLQLFLQTNLHELQFGDCSKLSFHGYQTLAIFTPNLKHLSLQMCGQLNNESLLYLADRLKDLESLFLDGPFLINEQTWIEFFTKMKTRLKHFKVSNTHRFTAPCLTSLLQNCGSTLETLGLSRVDTITNFREIPLYVNSNLSNLALEHPFNEDDVTDDMVIELVSKVGNLKELVLNGCSALTDSFIINGLCEYWDSPTNITLQTLELEELDQLENNGLPLLFEKIQFVNLQHLSLKRSIDLNDKTLLALLKNNLTESLLTLNLNSLNNITSECFDKQNLKFKKLQRLNTSFSKAINDTVLKNIISEVPTLRIMEVFGCSRVDDKFNVPANLVLVGRASDTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGH8</accession>
    <name>A0A1E5RGH8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1574</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGH8"/>
    <dbReference type="FunCoup" id="A0A1E5RGH8">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGH8"/>
    <dbReference type="InParanoid" id="A0A1E5RGH8"/>
    <dbReference type="OrthoDB" id="68611at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018823">
        <property type="entry name" value="ArAE_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023244">
        <property type="entry name" value="Brefeldin_A-sensitivity_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052430">
        <property type="entry name" value="IVT-Associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049453">
        <property type="entry name" value="Memb_transporter_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47804">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47804:SF4">
        <property type="entry name" value="AFR661WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10337">
        <property type="entry name" value="ArAE_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13515">
        <property type="entry name" value="FUSC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02047">
        <property type="entry name" value="BREFELDNASP4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="616"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="647"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="674"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="698"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="724"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="757"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="domain" description="Putative ER transporter 6TM N-terminal" evidence="7">
        <location>
            <begin position="20"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Integral membrane bound transporter" evidence="8">
        <location>
            <begin position="641"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="962"/>
            <end position="981"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1179" mass="133038" checksum="303F982BE57855CC" modified="2017-01-18" version="1">MDSNFIKSVRKLQQKYYPCDRMLLKRLIKCCVHSTLCYICCLFPPLRDRLGLEPSFLPLISVIIHPGRRLSATVQHTIKCGFGLLSGLCWALLGREIAQRCLGHTWNILSEAEHRELHYARYQAALAILVVFECIMLFYHGWMRSVEHTNFAMVFPFFLVVHFAFSESLKENAREIALSYTAPFFLGIAFALVWNIILFPDLGTTYLGTSVINSLNNIHDAVDDSVKFFTSANHFTKDSSFYLENYKSLLKYKNSVQSKISVTNLVYNECAFEISYSYMAPHSVKPIIDQFRNLSLCVNGLINACQLEFFAIDNDNSKDFIQTRREVIFVDHDRLLKILDILEPCVNDLGSKLSHALYQAKIALAFSYDVSQKDVKASNSFPFAGDVAIGKNQNANHFDEVTESIELALKVFDKTFKNELAGLGYDMLTPSDEMFLLSSFLMNFKQCARTIISVVKETNVLYTKRVERESKGWIRGKKLWFNFMGSFKQFVTFVLGGSPHRSGKTPTSRATHSVTESQALQGNVGNNVTLEYEGSRGGPNEGIAQRPKFEEEQLLGQSEDMGIEKIESLKRKETLPVTSKTGSEVNADETLTLNEKILHAIEFFSFQCRTSWRPHFIFGFQVTVCLTIVSVPMFLPEGRAWYGHMHGPWMAFVAILCMEPNVGGTFWVSFLRSFGVVAGSAWAYLCYVAGYHKSNDPYLCTVLTVFGAFPGFYYFIATPYVKGAIIYIITIYIVLLATIIPSKTLKSGILQNFAKRCFAVATGAPVALVTQLVFFPIQARDCLNEELSFVCGCLSQMILLLETGLESDSLSSMNFTSEKYHRFTTLSSAAKAALRRATEYKGLTRQEPRIKGEYINIEQAQTDIILLMKEIVERLDNIGNVTIHFGSDMLKDVNKDLHAYRRQGVASLLSVIRALGESYLNKTPLPPFLPSARICHRRFVNRIKEVMDQNYGHVIQRMGLVETPNDSDSDNESDSKEDSNDDMLMFARKRKLVISDNTRSKEVLRDTLLSWNARNANFEEVIEYVEELVVFTKKLVGSTDFQYGFLSRPLNAEWAAQAVTDFDDYAQNGTSPSKLQAALSQSSKNERINDNDVDSSVLIRMPTIQKSTTLDMKTKKRAYSIGSIDTHTAEALAHIHDPIPSALDSTSNKSTSPSLQETTIKATELPPILKNVFVNKRND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGH9</accession>
    <name>A0A1E5RGH9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1089</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RGH9"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="unsure residue" description="D or N" evidence="1">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ86011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="218" mass="25005" checksum="A6DA095788982204" modified="2017-01-18" version="1">MTEQALSVHSVQTFQFDPXNRYHLVHIHMHVDLNIQNDHLDDHEXLANLTKILSNLAQKQKVVQQTPQQVNILVFPYSQEFTLRYWMALTNQYNXMECHGTRLRNIIVTVEQNTKKQTXNSHDMTTSLITYASKYTVLNNXNACNSAVNFGVPIDMDAIIATLLEKEGLCVKSXDSGFSLRXSLIHSSXQAEKSEELQEILDQIYSKFDNLQEVMESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGR2</accession>
    <name>A0A1E5RGR2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nitrogen network kinase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1492</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGR2"/>
    <dbReference type="FunCoup" id="A0A1E5RGR2">
        <property type="interactions" value="256"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGR2"/>
    <dbReference type="InParanoid" id="A0A1E5RGR2"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="488"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="700"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="791"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="938" mass="106417" checksum="D875738F42DCCD43" modified="2017-01-18" version="1">MSHLQNINNSKKTSHSAQEIASTNPFADHVQQGTKRRQKLFRVYSENEKTNFAQETGHRLYDDDKYRPLHDKEHIFQKRHFSVNEEDRPNMDFNVSKDNNTISNYHPSYNDSNYKLKSFRHQGSLFDSDGDDDEEEDDTSGEQCRNGRGANLEDNEEAYNKFFTDSIELDNECVIPLDKLPRLKSDQSLKRKPGIAGKKRMRRNSDDMFDEYIPDFDMINNLNRLAQYYELNGSHAGSQPQLSQEIEEAKENDEQEQQDILSKTNITRHNRTDALKVGLSKQIGKRKDNTNYGNKEESQIRQSPRVAALTNASSFSSLKSILEHSKATPIPVQFSDRARKRLPKTGTDSLESTYVMRTGKSHDFELDLSKVPDNFDSLPYSQRKKTILTLFPNQDFKTVLEEIRSPKNKLRRRANTLTSNTSSSLSTKSLTYSSSANAQTLLQQFNAPNVDGLGTAGSSSSSLAECDRNIDQSTKKKSISKGLELMGYQLDKIIGYGAWGVIRECHSLTDKSDIKAMKIVRFKHHAEVRKSVLNEVNIWKQLHHKNILSLVTYHYDDHAMFCLMDKIQGGTLYDIVAKNWHLNENLDLWTRSKEVVQFALQIIDALRYLHTEKRIVHGDLKLENCLINEIDNRILLCDFGMAQSCPENTGSLSTQAREIIGFENSTQLSSANSSAPSSSSSSSSSLLSLSASRGASQIAHHKIRKLKDSSQDTPRCYGFKPSSSTLKVPVLHNDHSKSHSLMGITSFHSNYGPALQSLTLSSETILPSNLASRNSNSSLTQLQNLRESIKSPIMPEQHHHKQKHKRPEYSRIDDIENNDSSENQIGSLPYAAPELVANVSNLISFETDIWALGCMLYCMVLGRLPFQHEYEPRLRLMIESGKYNKALLKEALGPVDKYLYQAIEGCLIKDLQDRWNLEKVAKYLNKSAQKRVSAVNPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGS6</accession>
    <name>A0A1E5RGS6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ARS-binding factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGS6"/>
    <dbReference type="FunCoup" id="A0A1E5RGS6">
        <property type="interactions" value="1382"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGS6"/>
    <dbReference type="InParanoid" id="A0A1E5RGS6"/>
    <dbReference type="OrthoDB" id="4065241at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006774">
        <property type="entry name" value="BAF1_ABF1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04684">
        <property type="entry name" value="BAF1_ABF1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="439"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="583"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="686"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="479"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="586"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="686"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="713"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="729" mass="82694" checksum="B501FFEEA3B569E3" modified="2017-01-18" version="1">MAPTYRQKIRILRLGENSYEFEDALVNRELTEKRFDSLSHFHNEVLNPYELKFKIPTILKNSHKMRHFTFACSCTDCPFTIMLSTTKNFGNTEFNIHDSTKEFSGKPSQSSFYSNTTSTAPTTLNGKSIATNDGAPVTGFSSFSVIKPAISSNKVKKQDFFYKDKKSGNNKSSSKLKNEEENIFEGEGIKVRLEESDNDGDGDGDGDGGEHSQSTSSRKGIAGFKKSKNKQQNADIEDDEDLTDTEDEERRDIPNLEQNMEDEDVTKQYGPFAVTKILLEHNHSVNPNVNSIEGLKEFILIKIPKVLHDDLKFDRLLKDLFENTVAKDLAKQYGIENTTNYMGKDNILKEKSPNTTLFSSQNSEDKKFKIAAYIRESGLLDVLEKKYVLKPTDVLADKAFSKSINRKITTYKSRLFTRLKKEFVANREKMISEQLEAIQQETDRGKNNRTSNNGSKKGQKKRKSSTALDETSSKRKQPKSNERKENNLMRVENDESQLISDALNATESLGANNIDTSLTETSPENIDFVLKNTRHLTNPTMVNNNVTEQNTLTGIDKHQDETLNDKKTQEAWALLKSHTEAFATMNKKKSSKSSSSKHSKKKKKKSSSKHNTVDDAATAVAAVAAVEAVAMNSDLPLSDHHIDHHIDHHHHHHHHLEDTEGMPEDLKNVVAHLDFMDSPLKYRSELEDRSDKNSETMMHERGFDHHERKNEDIGELVENDDNIQPELKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGU4</accession>
    <name>A0A1E5RGU4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cytochrome b-c1 complex subunit 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1421</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGU4"/>
    <dbReference type="FunCoup" id="A0A1E5RGU4">
        <property type="interactions" value="110"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGU4"/>
    <dbReference type="InParanoid" id="A0A1E5RGU4"/>
    <dbReference type="OrthoDB" id="2391627at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019182">
        <property type="entry name" value="Cytochrome_b-c1_su10_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28254">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28254:SF1">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09796">
        <property type="entry name" value="QCR10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ86107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="78" mass="8738" checksum="40BFFAE38847AE7A" modified="2017-01-18" version="1">MVAYVSKFAAKTAPHFGSLTLSNLINYTPNLILWGGASAAIIATFTEGWPKFQDTFYKKIPYFGSHWIVEKDPEDLPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGY1</accession>
    <name>A0A1E5RGY1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Alcohol O-acetyltransferase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1136</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGY1">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGY1"/>
    <dbReference type="InParanoid" id="A0A1E5RGY1"/>
    <dbReference type="OrthoDB" id="3979966at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052058">
        <property type="entry name" value="Alcohol_O-acetyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010828">
        <property type="entry name" value="Atf2/Sli1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28037">
        <property type="entry name" value="ALCOHOL O-ACETYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28037:SF1">
        <property type="entry name" value="ALCOHOL O-ACETYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07247">
        <property type="entry name" value="AATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ86145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="562" mass="64934" checksum="CB761C744D547D3A" modified="2017-01-18" version="1">MAGSYVRWTPNIDPQMLEKGHARQLGHLENYFVSCQRQRLYLNFSVSCDLTASAHPCGGKTPAITFTSIAEALKYTVFENPLLLTTLVEKYDDKDFYSKPEFLNTPYKDDFDYMKIVDKLDLKDVLINLQPEYEPFVQAAYELLNGASEKSSNTEEKMMELCNRLVIPYNKPDSLHWRLFWIENTNKFYCFTDHISWDGMSMVNFFKDFCAGLNQSQNTSFTSDSLNNTQIFNYKADHLDLRPLPLPAEKMVYYKMRALGLPRFICSEIIKAKLVHRNHSAKIHQIEVDQTLDSGCLAKNMSDQFIFRNLSIEKINRILQKCRSLNITITPMLQACWVKALXEMGEYENRYWNEWFTDIVVTSSAREYVDSSSCLSKFDEKDYKIGSLMAGSSYPYLIYEIMQKNVLELSKSYHQTFIMAKEKGHYIEGLGVFMQPFLYDKQNLDKIQLDRHIFPGSCKSGVMIGNLGYHKFGQTLKDVENKGKPGNSKSSFVIKDLKFIQGTASFTKSYVINIISTDLSGMNFVFCCDKNVFTKSKQTDDTDPFTKIVDNFFSKIEEFLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGY6</accession>
    <name>A0A1E5RGY6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Spindle pole body component SPC42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1300</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGY6">
        <property type="interactions" value="183"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RGY6"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="225"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="459"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="542"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="87"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="225"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="248"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="259"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="272"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="307"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="318"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="461"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="509"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="542"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="563" mass="64426" checksum="067E17F263D3AEAD" modified="2017-01-18" version="1">MNHSPSPKRNWQSSTRDSNPYGSSAFRMNNMPYTSRPLNNGGIYRAMDENNDEFVVPDYKDNARILEELRDFKKTATVRESELYGKIDGLESLCNLLKKKLESYKNKCDNLESVENELRHAHDKRQEDLKKTIRDLENVIHDQEAKIVEQERTIELQRNTIKSHETVIQDQTKSIENQANTIKDHIATIEAQNATIKNQKQSLSDFEDMVANREKKIKALTESLESWNSKRVHSTTGKSLSHNTPSQKIKRKSSANRKHISEQYVHDHENEFSDEDNVSEALEYDDEDGYDYEQTSDDYDSNIQKPLQHRGSTKKRNLPTSSCKTLNIEKNSKLRSPISDASSPRDSTVNKLLQKVDTLMNYLEDCTKNNKVPNFVDGRVASSTQGLHSEYSPNNLSSTVSSRKPTENDILLFENEELSDLLKTVTDMREKVSLRKKAEIXRKLLLQEIADLHKEMEGNKESTPVFKNSNNQEIHSHTHSXXNETDDHMHGTGCPQCESMAPRNKKSNDSASFPNNTRVNGGVDRSNSVIVNEEPSLTNISRKANSKRFSSQPTDYIHDNFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH31</accession>
    <name>A0A1E5RH31_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">T-complex protein 1 subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1125</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RH31"/>
    <dbReference type="FunCoup" id="A0A1E5RH31">
        <property type="interactions" value="1332"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH31"/>
    <dbReference type="InParanoid" id="A0A1E5RH31"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03337">
        <property type="entry name" value="TCP1_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000085">
        <property type="entry name" value="T-complex protein 1 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000005">
        <property type="entry name" value="T-complex protein 1 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012719">
        <property type="entry name" value="Chap_CCT_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02344">
        <property type="entry name" value="chap_CCT_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ86197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="546" mass="60529" checksum="AE0559E40500A8DF" modified="2017-01-18" version="1">MQAPVVYMNAPQERTVGHQAQISNITAAKAVSDIIKTCLGPKAMLKMLLDPMGGLVLTNDGHAILREIDVAHPAAKSMMELSRTQDEEVGDGTTSVIILAGEILTQCAPYLVEKNIHPVIIIQALKQALTDALQFIDEVSKPIDLQNDKAMKELIKASINTKYINHWSEKMCELSLKAVRMIKTGDEDAGLQNTSSEGVPKTFNKNVQIDIKRLIRIEKIPGGDVLEDSKIIDGCVLNKDIVHPKMKRFIENPRVVLLDCPLEYKKGESQTNIEITNESDWNRILQIEEEQIKQMCEQLLEFNPNVVITEKGVSDLAQHYLLKGGCSVLRRVKKSDNNRLSKVTGAQIVNRIEDLKESDVGTNCKEFKIELIGDEYFTFLHKSTNPQACTIILRGGSKDILNEIDRNLQDALAVTRNVMLSPSLSPGGGATEMAVSVKLSEKAKQLQGIQQWPYQAVADAMECIPRTLIQNAGGNPIRVLSQLRAKHAQGLYTMGIDGEMGKVVDMCQYGIWEPEVIKQQSIKTAIESACLLLRVDDIVSGVRKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH54</accession>
    <name>A0A1E5RH54_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-(apurinic or apyrimidinic site) lyase</fullName>
            <ecNumber evidence="2">4.2.99.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1206</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-1 OGG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RH54"/>
    <dbReference type="FunCoup" id="A0A1E5RH54">
        <property type="interactions" value="609"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH54"/>
    <dbReference type="InParanoid" id="A0A1E5RH54"/>
    <dbReference type="OrthoDB" id="238681at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034039">
        <property type="term" value="F:8-oxo-7,8-dihydroguanine DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012904">
        <property type="entry name" value="OGG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052054">
        <property type="entry name" value="Oxidative_DNA_repair_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10242">
        <property type="entry name" value="8-OXOGUANINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10242:SF2">
        <property type="entry name" value="N-GLYCOSYLASE_DNA LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07934">
        <property type="entry name" value="OGG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="11">
        <location>
            <begin position="180"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="329"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ86230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="413" mass="47345" checksum="782B703DD63D46A3" modified="2017-01-18" version="1">MALTENDIGTVQICCFLIKRTSPKLTASLQDTEIKMTALEWLKVPCKKDFLDISIILQIGQSFRWIYNPLTQEYLSSVNLKRSDCFAVVKLKQDEHHIYYQLCPSLKKEESQDGVLALNSQVQDLAQWVHNYFRLSLKREALFEHWAKVDLKNAESFYKLRSSCCVLHQDPWETLISFICSSNNNISRITKMCHKLTELAGTPLGYTENGVDLHAFPTSTQMISCLSEASLRKWGFGYRSKYIIDTAKLIQEFKEADKESFKSDSQYLYDKLHGIPYLEAREFLLHFPGVGPKVADCVLLMGGFGFDEVVPVDVHVKRIAERDYRFGSKKQTKARLEELKLEYVKYKNTKKKINYDLDAIRLQLIDLWGPHAGWAQALLFTKEVGSTSGKVGDAITKRKIDIAQEPTSKRSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH79</accession>
    <name>A0A1E5RH79_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="3">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1114</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase delta/II small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH79">
        <property type="interactions" value="874"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH79"/>
    <dbReference type="InParanoid" id="A0A1E5RH79"/>
    <dbReference type="OrthoDB" id="3763at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.430:FF:000002">
        <property type="entry name" value="DNA polymerase delta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040663">
        <property type="entry name" value="DNA_pol_D_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024826">
        <property type="entry name" value="DNA_pol_delta/II_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416:SF0">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18018">
        <property type="entry name" value="DNA_pol_D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA polymerase delta subunit OB-fold" evidence="12">
        <location>
            <begin position="53"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="11">
        <location>
            <begin position="234"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="194"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="13">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF18018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ86234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="468" mass="52771" checksum="72D8C3EC9A086784" modified="2017-01-18" version="1">MEALLAKYNQNLSDDSXIXNKDVQVIPPIQESTLINNENPFYLPYNNRAYDKQFFKLYQYRLNVLRKRVYDKAHKLWQDHPYMKKVLDIEYGSACWSIGAIYCEMKYKPDVLEEVSNDVYGSPDLITKYSDEQNDEIMLEDESGRVLLKNLKKKFVTGTVVGVLGQENDDGDFEVLDICFPQDLPINECIPTTSTTTSTTTTTSTTTTTTTTTTIPLDSTPSFPSSSPSKEGNVAFISGLNISSDNLSSVQLLQDYLIGALSLTTHNISKLYILGNSIKDIDNLTNLQHFLINIVQSVDVQILPGFHDPSDKSLPQQPLNDKLFAKKNLHNYLELTTNPSVSIIENNNMEMLLTSGENLNDIFKYNYIQQTRQQMMELTLQCQNIAPTAPDTLFCYPFDDKDPFVLDKWPNVYVVGNQPSYFEFMTTSIGGNTSSKGIKCIGVPDFSKTHGLVVFNTNTLYTELVSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHK4</accession>
    <name>A0A1E5RHK4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AP complex subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1474</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHK4">
        <property type="interactions" value="845"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHK4"/>
    <dbReference type="InParanoid" id="A0A1E5RHK4"/>
    <dbReference type="OrthoDB" id="10254310at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030117">
        <property type="term" value="C:membrane coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026739">
        <property type="entry name" value="AP_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016342">
        <property type="entry name" value="AP_complex_bsu_1_2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11134">
        <property type="entry name" value="ADAPTOR COMPLEX SUBUNIT BETA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002291">
        <property type="entry name" value="AP_complex_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="8">
        <location>
            <begin position="25"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="645"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="645"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="667"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="694" mass="78805" checksum="E42E3F6ADE950692" modified="2017-01-18" version="1">MGPFDKKLKKLISNAVRVAPKVSAKGDTTELRDCLASSYPQTRKDAIKKTIQHMTMGKDMSSLFPDVLRNMATSDVEQKKLVYLYVMNYATSHPELCILAVNTFVSDASDPNPLIRCMAIRTMCMIRVDMIIEYVESPLRACLADENPYVRKTAVICVAKLYQLNKELCLNLMENDLIPMIRNEDNAIVLGNALASCMELQSLINYKQVVYDNIMKFLNILNESSEWTRITILTAIADYEAHDESESLKIIDRVVPHLQHVNPSVVLSSVKVVMKNLNFVNNNSKKNGFLEKLATALVSIMSTPFEIQYVALKNIRIILEKYPTILSKEYRIFYLKYNDPLYIKMEKLEILVRVVLPNNLKQCQTVLQELKDYSTTEFNKELVSKVIRCLSQLAIKVPEVIPSVIDFMTTELSMRQDDDDVLIALCSMLRNSTAATTTTTAYKTSIKQIISYAITIYEDRLIKDEGKCNFIWLIGEYPNEFGGNSQLATVLEYYLDSFNEEESMTQMALLLTIFKNKKYLSESLLQTVLQKATQESNQLDIRDMAMMYWRCLSLDTGTNVAENIINDFTQIHVPTTDNTIDKFPPHLLELLLQQLSTLSTIYFRPNNDLVLQEHSNIVRGKGMNELADIARLEISKNNDLLLDFDTDPSTPTTPSTSTPLDDLNDLFGSSKSKPVSXSNTGMTNNGTKDLMDLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHL5</accession>
    <name>A0A1E5RHL5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Recyclin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1536</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHL5"/>
    <dbReference type="FunCoup" id="A0A1E5RHL5">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHL5"/>
    <dbReference type="InParanoid" id="A0A1E5RHL5"/>
    <dbReference type="OrthoDB" id="5554140at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009976">
        <property type="entry name" value="Sec10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048627">
        <property type="entry name" value="Sec10_HB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12100:SF1">
        <property type="entry name" value="RECYCLIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12100">
        <property type="entry name" value="SEC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07393">
        <property type="entry name" value="Sec10_HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exocyst complex component Sec10-like alpha-helical bundle" evidence="2">
        <location>
            <begin position="255"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="365"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="803" mass="94387" checksum="73FED900E6938B9B" modified="2017-01-18" version="1">MADNILTFENLAQVDSILQRITSLLSIDDFLSLQQTNKYIFQRCQKLRIEETKYKRYFQQWRIPIVETEDVLVDQDLPFNKFNEVNAFQKIKTVPDKQMKPYFVFLYETFEPYIKKLSNNQINSKFFTKTQDPINQANILNNILKFNAINKTDPLFSQQIKERYDILKDIFVNSILRELESPQTSSAVKRKFVSVLLVLHEYNISIEYFKSLSDFQTDVLQGVQLFSSEGTAAFEIDIVGWSTFWVKLSHWFNSNIKLCDDLFEDDYPVIINFAEDIINESLLRFIKVEFQATNIQFFDKFYQSLQTEFIDKLEDSKNAGGLQKLKEMIAQFIILYFEPFITSFTSFLVNRFHEQVLTKLTEFSEEVATQELKQNEQLYESIQKQYEQRRLEKEEYLDNKNNFLRSFQQILKINNNKLDKLEKQNLEVEFNLNKMNKSLENIKSLIDLNLCMEAISLAKTDISKILSFKKLGKYENTMIIEKNCENIFKILITEIQNKHVSPAFEKTLELLKKNNEEINTSNLEAESFQTSEPLIKFTELINIGDVIIQLIAIFFKNEILEQKIIRKKDNNDFLNDVIKLKKDFETQIDDFVAEGLHIGINKLMIEVEYVFNSTQLPTDYNPDGFQAGQEENEDMHHDVFVNNNTALFRDVVPSECCLKVVELIKNYCFLLDGVTAKGTIEVYQEEIGDRFFQSIVKNLMKNLISKNGAIYLICDINYYYTFVSEELKLRKVLPLFESLKCISQLFLIDGNGKELGKMICDSSRYNGIFTQEEVYEFVTRRSDWHKVKRQVDKTIYGLDCVIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHM4</accession>
    <name>A0A1E5RHM4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1538</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHM4"/>
    <dbReference type="InParanoid" id="A0A1E5RHM4"/>
    <dbReference type="OrthoDB" id="4067302at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032299">
        <property type="term" value="C:ribonuclease H2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09271">
        <property type="entry name" value="RNase_H2-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.680">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013924">
        <property type="entry name" value="RNase_H2_suC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08615">
        <property type="entry name" value="RNase_H2_suC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ86387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="139" mass="15932" checksum="B6B6BFA5429B586B" modified="2017-01-18" version="1">MTNLNDTQKKEITFNSARQLPVYDAHLVPCIINYDGNTSELPKNFDSETEASKNIATESLDKREVKYLRGRKLVGQEVNLATPENSNFQTYIFDTDTNQICSKVSALYNYEREGNEKRLIAESARFHELLATMETIMDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHR4</accession>
    <name>A0A1E5RHR4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">U3 small nucleolar RNA-associated protein 21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1250</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHR4">
        <property type="interactions" value="1175"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHR4"/>
    <dbReference type="InParanoid" id="A0A1E5RHR4"/>
    <dbReference type="OrthoDB" id="10250769at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059157">
        <property type="entry name" value="WDR36-Utp21_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007319">
        <property type="entry name" value="WDR36/Utp21_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22840">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22840:SF12">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25171">
        <property type="entry name" value="Beta-prop_WDR36-Utp21_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25168">
        <property type="entry name" value="Beta-prop_WDR36-Utp21_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04192">
        <property type="entry name" value="Utp21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="WDR36/Utp21 N-terminal" evidence="5">
        <location>
            <begin position="57"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="606"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="WDR36/Utp21 C-terminal" evidence="4">
        <location>
            <begin position="741"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="6">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ86441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="965" mass="107007" checksum="C8F2D983912A03A1" modified="2017-01-18" version="1">MTQADFEVLKKRKTTVNNGGSSLTKHASKIFSPFRVIGNVSNGTPFAVGTLGTTFYIVTSVGKSFQIYDANNLHLLFLSEGETESLITCFATHHHYVFAGFGSKVGVYKRGKLEFLLELEEAHSGETVEKITLFGDFVVISTRESSNIYVYKKEKTDFEPXSNKTSISYQLYTTLRNITDSVSKNAIIDIIHLPTYLNKIVVVTQRNVVLYNVRTGKNLYSSEEFATEITSVVVAPALDIIALGSANGEVXLYNVKKGRKLRTIKPHSVVRITSLSFRTDNLPHLAIGYANGDLVFYDLNRRSRVHVLTSVHKEINGGIAKAEFLNGQPIIITTGGDNQLKEFVFDPPLTENNNRDKENDDEMGDVVQPPRLLRSRGGHSKPPTKIQFADNEAHFILSASSDSSLWGFSLRKDAQAQEFSQRAANKGKKSKVYSKLPTVXDMSIELARAGEWDNLVTAHENSSVARTWNSKXKRLGSHLLKTGDDGFVTAVHLSTCGNFAFIGSSKGSITVYNMQSGTLRKKFNLHKQSITGICVDSMNRKMVSVGLDGIVGFYDFAKSKFLGKLQLESPITSLKYHRGSDLFALALDDLSIVVIDSESQKIVRQMFGHNNRITALEFSPDGRWIISASLDATIRTWDLPTGTCIDGVRVDSVVTNLCFSSNGEYLATAHVKGNGISIWTNRAQFKAVSTRQIDESEFQKIMLPNSTITGGVSMLEGALGENESEEAADDQYFGTYQSLAQIDEDLVTLSLGPRSKMNTLLHLETIKKRSKPTEAPKKPEKLPFFLQLTGSAVGDEASSREGALNXSAETAANIAETKKKETEATENLNKFKPTGRLGFESKFTNLLRESARKQDYTEFLELLISMSPAAIDLEIRSLNSFEPFEEINWFIEALTAGFESNTNFDLLEAFMNMLLKVHGDVIHSHSEAISPSLNEWNKVHKKKEENLDDLVKFCSSVVNFVNTSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHS4</accession>
    <name>A0A1E5RHS4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">H(+)/Cl(-) exchange transporter 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1654</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHS4"/>
    <dbReference type="InParanoid" id="A0A1E5RHS4"/>
    <dbReference type="OrthoDB" id="44789at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03684">
        <property type="entry name" value="ClC_3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3080.10:FF:000030">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711:SF3">
        <property type="entry name" value="CLC CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0868">Chloride</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="246"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="402"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="452"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="489"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="530"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="572"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="630"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="655"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="100"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="10"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="130"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="987" mass="110515" checksum="78E1465A376BB09B" modified="2017-01-18" version="1">MESTKHNSQNERTPTDQQKFATSLQQKLRRVPLQTQKSQTLLRESLPGIDQLVEEPNAYSQTPSPRNNSNTQNITNKPLHLPSKELVDFASQTSLRTPIKKFSLPGNVSDSSSKDQNDSRVTKSISNSIDNLTADNLANNLKSEERSTTVRPRKSRAEFYLSTPNAEITDPLLSSTYHPNRYGATNSASSDKSSSELQPEVPTKTRNFYDDFTSVDWVKDYIYDANSKATPTGNFFRLMLHRLTDWFALVIICLVWACIAYVIDKFEELLIDFKKGVCFFNLLLNEQQCLKSNHEWVLWPQVIYEKLYRFDQSFFPTVDFLFYVTLSILLAYFSVKLTLTTKNINPLTTQHSNDLSKTESNFDMATKKEAKVMYSAYGSGVPEVKTVLSGFTIRKFLGSYTLFAKGLALVFAIASGLSLGKEGPYVHLATCCGNICSRFFTKYKENGIERRILLSASAAAGVALAFGSPLGGVLFALEEVSYYLPGNQLFKTFFAAIVSTLFLTFLNPYGTGKAVLFSVNYESDWVPLELPLFTLIGVGGGVFGALFCKFVQFWGGWFRSKKFVKNKPIREVLIIALLTAFLSFANPFTNTSVASVLASVASPCYSMDDSTGDFGLCPLKSMNFMSEFNSMLYALVVKIGLTAITFGLKVPAGIYVPSMIIGALGGRLFGMFIQYVAYSHRGVFPFNEICSKNTTCDGLCVDMGIYAMIGAASTMAGVTRMNVTLAVIMFELTSSYNHVLPMSIAIACSNFVANFLEPRSLYENLIIKNDLPYLNKQKPYCISKDHQQDLKYIMTSLNENKKTGFYIDVSKSNYVPSNELRCLLEKQQRRGLVDGCIPILKNDKLVGILPGPELELALDKVSDFRLEYKLPNDVQLKLFNDNSDSITPDSLRNEDINEIVQSLSGTNTQDSHMAEELLLVFMLNKISDLTDIVETAPIILSINSPLSLVELVFINMGNRYIVIMNEDQFVGFLHKKDFIDYCRRLET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHU1</accession>
    <name>A0A1E5RHU1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">rRNA-processing protein EFG1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">rRNA-processing protein efg1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1030</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EFG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHU1"/>
    <dbReference type="FunCoup" id="A0A1E5RHU1">
        <property type="interactions" value="184"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHU1"/>
    <dbReference type="InParanoid" id="A0A1E5RHU1"/>
    <dbReference type="OrthoDB" id="47732at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019310">
        <property type="entry name" value="Efg1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050786">
        <property type="entry name" value="EFG1_rRNA-proc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33911">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN EFG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33911:SF1">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN EFG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10153">
        <property type="entry name" value="Efg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="131"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="213"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="48"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="213"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="233"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="248"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="258" mass="29447" checksum="7FC7B9AB25FC0310" modified="2017-01-18" version="1">MAKSYRTHNASIQVASVIGAGTNKIKKKIRDIERLLAKKRDTLPDTVIVENERTLDALRLELKKSELKRLAQKYSKKYHMVKFFEKKKALKLYNRELKQKPINKETIVERAVDLCYTVNFPKTEKYVSLYPKSSKSKKKQSNSEKEGTGNDDNSSDDESENTAEAPETVQKREFFRELIRKHYLNKTLPLGVNEILNGKTLPNEDLGIVVASYSTEDSQPNSTPKLSTEKKSSQSSQDLQKNSKSNSDSDDESDGFFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHV1</accession>
    <name>A0A1E5RHV1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Protein transport protein SFT1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1385</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHV1"/>
    <dbReference type="FunCoup" id="A0A1E5RHV1">
        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHV1"/>
    <dbReference type="InParanoid" id="A0A1E5RHV1"/>
    <dbReference type="OrthoDB" id="3063237at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15853">
        <property type="entry name" value="SNARE_Bet1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039899">
        <property type="entry name" value="BET1_SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12791">
        <property type="entry name" value="GOLGI SNARE BET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="10">
        <location>
            <begin position="7"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ86470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="97" mass="11150" checksum="F7C9A6C85DB3DE74" modified="2017-01-18" version="1">MSDSRYQQLENNNNQRLDELASKLSTFRQINSDIHLQAQDDMGVLDNLSNHFSSLMSNLKNSSTRLTRSMRAGTGIWKMVGLALVIFFVLYTLYKVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHV6</accession>
    <name>A0A1E5RHV6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Protein PBN1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1651</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Required for proper folding and/or the stability of a subset of proteins in the endoplasmic reticulum. Component of glycosylphosphatidylinositol-mannosyltransferase 1 which transfers the first of the 4 mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase GPI14.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endoplasmic reticulum membrane</location>
            <topology evidence="11">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type III membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the PIGX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHV6"/>
    <dbReference type="InParanoid" id="A0A1E5RHV6"/>
    <dbReference type="OrthoDB" id="5546453at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990529">
        <property type="term" value="C:glycosylphosphatidylinositol-mannosyltransferase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000030">
        <property type="term" value="F:mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042322">
        <property type="entry name" value="Pbn1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013233">
        <property type="entry name" value="PIG-X/PBN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28533">
        <property type="entry name" value="PROTEIN PBN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28533:SF1">
        <property type="entry name" value="PROTEIN PBN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08320">
        <property type="entry name" value="PIG-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00780">
        <property type="entry name" value="PIG-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 11" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="543"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="226"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="572"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="578"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ86456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="609" mass="71630" checksum="429AE3A190C74DD1" modified="2017-01-18" version="1">MQKRTGRLTLLFNSKEDLYNQNEELVDIKDDGTVIINKENIVDGENEILVQNKTTLTNLFDKNGKQDIDNKELSNVLTKNYRITLKNTHERDDNNFLIEPFLSKGLNIYEYDLHETGNEQYTNASFNNYLLLDKLLKKYDIQMIFAKNKNYDILIQEQQQLNESDTEKDRITSKVEISEYYYLENKPPFVEMFQIDEDHFNDIGFFYIDSERLWNLINFSGLTCKQKSKPKTKTNSDVLDKNNNQKNNNNNNNNNNNNNNNKNKNKKNNQNSLQCNKTSFWYSQLFTNHKRLNNDDLDEQKIQNQETKNNKKQQHHQHYLDTMDYTIKEPKFDANNGLHPQFIINAKKYGNRFAKQLTPVNEGVYDNTKQMKCDYYLYLDLPNQIFLNKFDSEFENVSNELSPTNITTTPYLELIAQIGDSDLEKPDYQLPENGKTLNLIKLHMDQNSPNTTFHFNLHKRYINPVDKTQITPITENFQFDSYIFQKCPLDFENKGNPFVKKNHGSLLDNFLLSNNDKYFVDMIHSNTFQFTIPAPDINEYSNIYLWTWSIIGLSIAYLFYKMFSKETTPKKNLLIDTPRSSNDTPRSPNDTPKSPNDTPRSPNDTPRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHX4</accession>
    <name>A0A1E5RHX4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein STE50</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1624</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHX4"/>
    <dbReference type="FunCoup" id="A0A1E5RHX4">
        <property type="interactions" value="105"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHX4"/>
    <dbReference type="InParanoid" id="A0A1E5RHX4"/>
    <dbReference type="OrthoDB" id="445896at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01786">
        <property type="entry name" value="RA_STE50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-associating" evidence="2">
        <location>
            <begin position="228"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="210"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="318" mass="35773" checksum="9F0E908A772A0913" modified="2017-01-18" version="1">MLHGVVLPEGIASWNTQSLIEFLDFNLNKKRIKDGNNGEVIIEEDEPIFTTDMKDAFEQNKINGGNFMDLGVDDLKELLFLEQDASAETVQKQRRALVSMKLLINKLSNRKESSLIIDNLSSMYQVLNDKFLAYQNQYNKLRLDVLEIIRTTNNVAPTLEHSVSTPNVHSSSMDKFNKQNEIQGQYANSQPSLVQKASTYSLGQATIANGSNNYNGSSNPESSGEGQAGEPLKQLRASKEDSCEKVLKNAMRKHNLQDQDWRSYVLVICYGDQERILSNDEKPVTIFKSLKKSGLHPAIMLRQKDDMQLDSNTPGGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI44</accession>
    <name>A0A1E5RI44_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">KRR1 small subunit processome component</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">KRR-R motif-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g1659</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly. Essential for vegetative growth.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Interacts with snoRNA U3. Interacts with MPP10, KRI1 and with ribosomal proteins RPS1A, RPS4A, RPS4B, RPS8A, RPS8B, RPS11A, RPS11B, RPS13, RPS24, RPS25, RPL4A, RPL7B, RPL8, RPL23, RPL25 and RPL28.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
            <location evidence="1 11">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the KRR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI44"/>
    <dbReference type="FunCoup" id="A0A1E5RI44">
        <property type="interactions" value="1155"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI44"/>
    <dbReference type="InParanoid" id="A0A1E5RI44"/>
    <dbReference type="OrthoDB" id="441223at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22393">
        <property type="entry name" value="KH-I_KRR1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22394">
        <property type="entry name" value="KH-I_KRR1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000011">
        <property type="entry name" value="KRR1 small subunit processome component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000014">
        <property type="entry name" value="KRR1 small subunit processome component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041174">
        <property type="entry name" value="KRR1-like_KH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048550">
        <property type="entry name" value="KRR1-like_KH1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048548">
        <property type="entry name" value="KRR1-like_KH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048549">
        <property type="entry name" value="KRR1-like_KH2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024166">
        <property type="entry name" value="rRNA_assembly_KRR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12581">
        <property type="entry name" value="HIV-1 REV BINDING PROTEIN 2, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12581:SF0">
        <property type="entry name" value="KRR1 SMALL SUBUNIT PROCESSOME COMPONENT HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17903">
        <property type="entry name" value="KH_KRR1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21800">
        <property type="entry name" value="KH_KRR1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006515">
        <property type="entry name" value="KRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 11" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="6 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 11" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="K Homology" evidence="13">
        <location>
            <begin position="122"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="234"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="279"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="239"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="279"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="318"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="348"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR006515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ86557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="358" mass="42004" checksum="A858303FEA64F8FD" modified="2017-01-18" version="1">MPSTHNRDKPWDTPDIDKWKIEEFKEDDNASGLPFAEESSFMTLFPKYREVYLKSIWNEVTKTLDMHHIACTLDLVEGSMTVKTTRKTFDPAIILKARDLIKLLARSVPFPQAVKVLQDDVACDIIKIGNFVSNKERFVKRRQRMIGPNGNTLKALELLTKCYILVQGNTVSSMGPYKGLKEVRRIVEDCMKNVHPIYHIKELMIKRELAKKPELAEEDWSRFLPMFKKRNVARKKPKNIKDKKISDEKKVYTPFPPAQTPRKIDLEIESGEYFLSKKEKDLKKVNERKEKQEERQLEREAERAKDYVAPEEEEYKSSLKKSKKDSKKSKKRSSSDNEEDDKKEKSEKKHKKKHKKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI81</accession>
    <name>A0A1E5RI81_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Protein-lysine N-methyltransferase EFM3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1178</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RI81">
        <property type="interactions" value="501"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI81"/>
    <dbReference type="InParanoid" id="A0A1E5RI81"/>
    <dbReference type="OrthoDB" id="3971695at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF130">
        <property type="entry name" value="PROTEIN-LYSINE N-METHYLTRANSFERASE EEF2KMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ86597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="375" mass="43106" checksum="E74C5AD52B4FC60E" modified="2017-01-18" version="1">MSFYNKXEQRYPIELFAEYIRSNGVYIDCATFQKVFETISSNKXYQKKLLKLFIDSPFLLELEENASEEENENMAELQEWVQETYLSFLNVQFDPDLVDVITYIIPQMSAXSPFCASNSLVRIKIREIPYKISGNNTTGFRTWEAALYLLFFLYGENSPISALKQKNPDRIPLDDGVKPIFSKPLRVLELGSGTGICGLALFKFPMGLKIEEVTLSDGSQDIVENIIPRNYELNKELQGDDKLPKNLSIKQILWGKDDFLPKLKHSYDIIIGADVTYDARVIPLLVESIDYYLNFEKNSMCLISATIRNESTIKTFEDSFVSIASESDTRFGFLKVEKISDTKTIDKDLLKTFQQKVWFRDMIAPISVYKITRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN85</accession>
    <name>A0A1E5RN85_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g924</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN85"/>
    <dbReference type="InParanoid" id="A0A1E5RN85"/>
    <dbReference type="OrthoDB" id="10253736at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ88342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="246" mass="28250" checksum="32DBE84A99B06CC3" modified="2017-01-18" version="1">MQSVYVINIDSPNDLFSSLSAGNYLQIDCTDFGNFEFLKECIDKMGKLISSTPKINQHSTIIVVNNVDTGSERNGGRMGVLDEEHCAFMHTTSSNATNVMLMFSEIITRFENSYFLYLINISNVVGLHPNDYRDHSYAYSCSKAAVIQFHQSLTSEVNQYKRAPFHTIKTLLVLLPFQKQETCMWKKLFSTLHTDKNKRSRKHVDKIIQALASGRRGEFHLTDGFVENIKLNAKYKFKHFNEIHSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RND0</accession>
    <name>A0A1E5RND0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosome assembly protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Elongation factor-like 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g669</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RND0"/>
    <dbReference type="FunCoup" id="A0A1E5RND0">
        <property type="interactions" value="821"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RND0"/>
    <dbReference type="InParanoid" id="A0A1E5RND0"/>
    <dbReference type="OrthoDB" id="364892at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01681">
        <property type="entry name" value="aeEF2_snRNP_like_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04096">
        <property type="entry name" value="eEF2_snRNP_like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01885">
        <property type="entry name" value="EF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16268">
        <property type="entry name" value="EF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16261">
        <property type="entry name" value="EF2_snRNP_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000006">
        <property type="entry name" value="Elongation factor like GTPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000746">
        <property type="entry name" value="Ribosome assembly protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000002">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF3">
        <property type="entry name" value="ELONGATION FACTOR-LIKE GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="18"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="577"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="87"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="588"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ88392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1157" mass="130528" checksum="67DBEE6AD30CFD61" modified="2017-01-18" version="1">MGRYISAETCAKLQNNPECIRNICILAHVDHGKTSLSDSLLASNGIISTKLAGKVRYLDSRPDEQLRGITMESSAISLYFRVLRKKEQGKEPQDNHEDKSKESTPGSENVTVNEHLINLIDSPGHIDFSSEVSAASRLCDGAVVLVDVVEGVCSQTVTVLRQCWTGNLKPILVLNKLDRLIIELQLDPLEAYNHLHRIIEQVNSVLGSFYTGDRALQDLSWRENLEQGNVSDFVEKDDSDLYFHPENNNVIFASAIDGWGFNVGQIAKFYESKLGINREKLSKVLWGDYFYENKTKKILTRKALNGKNLKPLFVSFILDNIWRVYETVLGEFNDDFDHHHELDVAKLEKITKVLNVQLLPRDLRTKDYKNLLRNIMNQWLPVSTAVLLTAIEKLPSPKESQKKRLDAILEFTPNSDLLDEKLRASMEQCDSNGPVAAYVSKILSIPREELPIKSSIHRDPSAVENDLFERARKAREEALKAGKNAEFTEKLAHASTDSNKPAKTFRKRGEDIYKRAEESTITPDIDELELNAKLAKKKQISIQLSESTPTTQARNDEFKIVTAPAPEFDLGFEYETEGQEIEEKNEEKNEDAESEEDDGEENEDFEIPEYIPTLDPNDPFSELFEYEDEDPFGNYEEDEDDENDNDSGEEVLIGFARIYSGTLKVGQEIQVLEPKYSPLRPKDFIHTTTITELYLFMGKELIPLDEVPAGNIVGIGGLAGKILKNGTLIETGVQGVNLAGSSLHSQPIVRVALEPSNPTKMGRMVKGLRLLNQADPCVETFVNDHGEHILCTAGELHLERCLKDLTERFARIDITHSDPVIPYRETFLETDDMNPTKNSEGQRGEVKFYLGKYRVELSTSPLKEETVKYIIQNENKIKLMNAKGFVDEKFKLKLTELLTPESVNNIVGFGGKRCGPNILVSEGGLLSKYFAPSSKASCLFADSIVNGFQLSCQEGPLCKEEVQGMLVVLKDVHEMTAEEIANVGIENYQVEIPDLSGRFITSTRQAIHQSFLDWSPRVMWAMYSCDIQASVDVLGKVYAVVQKRRGKIVSEELKEGTPFFEIEARVPVVEAFGFSEDIRKKTSGAAQPQLVFSGFEPIDLDPFWVPTTEEELEELGEFAERENIARRHMNTIRKRKGLLVDEKVVENAEKQRTLKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNH0</accession>
    <name>A0A1E5RNH0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein LUC7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g925</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Luc7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNH0"/>
    <dbReference type="FunCoup" id="A0A1E5RNH0">
        <property type="interactions" value="933"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNH0"/>
    <dbReference type="InParanoid" id="A0A1E5RNH0"/>
    <dbReference type="OrthoDB" id="153872at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006376">
        <property type="term" value="P:mRNA splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004882">
        <property type="entry name" value="Luc7-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12375">
        <property type="entry name" value="RNA-BINDING PROTEIN LUC7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03194">
        <property type="entry name" value="LUC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="293"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="137"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="294"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="324"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="341"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ88424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="390" mass="44542" checksum="EF88ED4E484F0BB8" modified="2017-01-18" version="1">MSASETQHSAIEQLMGHGALSKIVNTSTASNRDETSLESLITNHEICKSYIVAQCPYELFHGTKEYHGKCPQYHVSKYAVLFKTKFHDFANTNSGKNLGSLETSQKDLHVYLEFHKQYYQTLVGFIQKCNVTKEMAIEKLESKTEDERLKISEVSKELDNIDESIALLSREIEVLATNGELERSMAQSIYLSKLTIKREDVAKRVREFASKIATSGKQELQVCNVCGAYLSRLDNDKRLADHFLGKVHMNYVHMRSETSRLKTIFKKYKINPSSISSSIERRDTRSLSRRYMHQRNTTSLPSQSSTPITRAPGAGRYNSRYDRVQNSGSNFSSYPKRASRFQGNSNREYPSHQSSASTPTQSTRPYPPARFDTRGYRSRRSRQDPPPPTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNK6</accession>
    <name>A0A1E5RNK6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g967</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNK6"/>
    <dbReference type="InParanoid" id="A0A1E5RNK6"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004354">
        <property type="entry name" value="Meiotic_Rec114"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03525">
        <property type="entry name" value="Meiotic_rec114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ88478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="610" mass="70123" checksum="755B2A41543B596F" modified="2017-01-18" version="1">MDCSSTQSSKPLCHLFPIAKFSELKIESLSDLNNPKQTPIQRQQQLIKKAWVHHSSESSTDSLLYFQIDYTNANDFSSYLLTVIKIRNKNLESVETMLRITISELMTESRNDAYKFGSMNLSQTIRFNVKFPIVGCKYIKSGILNRFQINFLQKQNFDEAVNLFSKAGANLKDIGLLQTQQCSQFSQVITNFPTIHNNDKNSGRVQTQKQQTGGLPVQLQQVSNTQSNVNNQNEISSVSEITYGFQQSQATHYSTLPYQLSVPPKTEKSQNILQNPQSNYENVKKTSLPENRTLQNVNSIENFSLYEQKNNGQIPTPNQTCTFIASENNFVCQESPRNSSLNLDKMVQSNVNKKIEKYIESYFEQLSSKSEHRLIDNNQISNDFNNLSQLENTDENLKYQTFQKRSGSSDMISSKTHLHGFPPKLQSSEKQASPFRANEMVTLESYHTTKKRCKKSRSKQKSDNKTFKAIKKLDLRSQVVQFKLLESEKQVKVINKFSIKKNHDLFDELSPDHQAAVFEKCAEETKQFLQNNMSKKKLFALNLERQLQHLKALVSRNDVAPLIADIKLKYGMNTQYIERKISEKKFVEKVVKLNEFLALLTEKNDKKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNM5</accession>
    <name>A0A1E5RNM5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein HOS4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g823</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RNM5">
        <property type="interactions" value="156"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNM5"/>
    <dbReference type="InParanoid" id="A0A1E5RNM5"/>
    <dbReference type="OrthoDB" id="3973215at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050745">
        <property type="entry name" value="Multifunctional_regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24189:SF50">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24189">
        <property type="entry name" value="MYOTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="517"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="551"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="586"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="836"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="349"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="436"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="905"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1045"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1118"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1414"/>
            <end position="1475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="148"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="224"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1053"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1118"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1420"/>
            <end position="1432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1438"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1449"/>
            <end position="1468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ88450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1497" mass="168049" checksum="9B555BF5CA6E8050" modified="2017-01-18" version="1">MNTERPSNAAGAENANSSDDKANFSKETIKSQEPKRRSLSSYLTNRTKQRSSTPVPSGTPEDQKSSPKTSSNVEKTRSSYDLNSNQDVEGPAIEAFRQPTASEPFDLSTAILMSIQNKQQSEQQEKQREREQQLKKQNEALLRKQEQQRQQGAKFNQQQLTNTHKVATHDVYVQNKQQRNFQPPLFKSRFQPHQERSASQSGFIKSVSPSTEQGKSLSPKKVLKNADQENSDLRNDHKFQKPPMLQISNLSTISNSESQKKALSLAPCGNEKVPVEDKTLHVSFKPASVDVKSSQGTETLIRKENIQKDKAYVEPIIVSKSQAAISSTENGRLQGDSFKDSYSTATATFENNEISSDAETEPSHSPLKPRKGRLIRGDQISAEAHPRKPCDSPLKVSINESYFIGDSSESELSDINDLEEEVTGLNVGSSILASSPVKPNFLRPPTSAPSNVSGSDYAPVSIKKEKESSPDVTKWEKRLMGNYAKKKSTDDCFTEPKSLHVGKAVTKHTKHISRDPYGRSKLHQACIKGKREIVRQLVLEDGYSVNDQDHSGQSALHLAAANGHLDIVKFLVEQGCNINIKTRVEDYDTPLMDACLELEYEIVKYLLENGADAFYVNSIGDTALKYMENRIAEDDYEKEDEEDIFKVIKLLKQHMKIADEEDSVNLKLNSGAHSPALGEDEDEEIVLTPSGRRRNRSITPFNAAGNGDGVMNFDDITSAESYEKPKTMHGKMQDKFSLARTTKRFQNSDSSSDSDSSNEDLTFEVTDLGTKSGKNKLLKASQVGDYNYVGQYLQNGGRVDNRVFVAACENGHIDLINLYLGIGGADSNLKLSKRPGKPSILMVSCGKGHFETVRLLVESGADVNYKDEMGHTCAYYSQKGDNIDQQEYQYLEEFLLKEETKLKKESLGHVNGKPTEEESAAKLENTTTKSAGEEARLSESLSQVRKPATNPVLLKNEENESLADSKHYLEPAIKNPDPISSPSLQKFDDFKSTQDFAKKSSPSVVIKKVKLNDHSSAPTKISSVADEDLTKTTVFSKAEAHQATNNIAKKKDHKPEIADEEVLQDKEIVQNKNDAFERPVSPAAQAAIGISTTAQELGHLLKDDEKIEKLAKQELSDHADQCSEKDGHLNHAKQKTDAQLEEDEEYQQKIQEIKNKKKLEMLQQIKQYELQKQAEQEEKMKMEKLKLAEKEERKKKFELDQQLAKHRMIRSYYPLGLKNIFCPQNVASKSSDSLAVHATKMLDLLPIYYRKFDCDQNRYVLDLQIMSIHRGTLPEDLAKKNVEWKEIDFIWNFFKPLFLYVDEFGGKQDVSFGNIEAKPDNERAGLKCIDDNLRSPYTRSKVFQSLSEQIQFENAEKKLLYSLEMHWVKLDDVLNSLDAMFSGVDESAEIRSLIERKVASSLVEITTSVPPLLNQTAQPSLSSTTTDSTPSSAHLLSDRNSCSNNNIKHPNDNENDLRTNSLKVDKNDSTSQNEFPLRFRGRSSLLINNIIDPRPLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNW2</accession>
    <name>A0A1E5RNW2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Adenylyl cyclase-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g748</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">The N-terminal domain binds to adenylyl cyclase, thereby enabling adenylyl cyclase to be activated by upstream regulatory signals, such as Ras. The C-terminal domain is required for normal cellular morphology and growth control.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the CAP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNW2"/>
    <dbReference type="FunCoup" id="A0A1E5RNW2">
        <property type="interactions" value="783"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNW2"/>
    <dbReference type="InParanoid" id="A0A1E5RNW2"/>
    <dbReference type="OrthoDB" id="77251at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008179">
        <property type="term" value="F:adenylate cyclase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019933">
        <property type="term" value="P:cAMP-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.330:FF:000001">
        <property type="entry name" value="Adenylyl cyclase-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.330">
        <property type="entry name" value="Adenylate cyclase-associated CAP, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001837">
        <property type="entry name" value="Adenylate_cyclase-assoc_CAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013912">
        <property type="entry name" value="Adenylate_cyclase-assoc_CAP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013992">
        <property type="entry name" value="Adenylate_cyclase-assoc_CAP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017901">
        <property type="entry name" value="C-CAP_CF_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016098">
        <property type="entry name" value="CAP/MinC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036223">
        <property type="entry name" value="CAP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018106">
        <property type="entry name" value="CAP_CS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053950">
        <property type="entry name" value="CAP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036222">
        <property type="entry name" value="CAP_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006599">
        <property type="entry name" value="CARP_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10652">
        <property type="entry name" value="ADENYLYL CYCLASE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10652:SF0">
        <property type="entry name" value="ADENYLYL CYCLASE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08603">
        <property type="entry name" value="CAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21938">
        <property type="entry name" value="CAP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01213">
        <property type="entry name" value="CAP_N-CM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00673">
        <property type="entry name" value="CARP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69340">
        <property type="entry name" value="C-terminal domain of adenylylcyclase associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101278">
        <property type="entry name" value="N-terminal domain of adenylylcyclase associated protein, CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51329">
        <property type="entry name" value="C_CAP_COFACTOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01088">
        <property type="entry name" value="CAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-CAP/cofactor C-like" evidence="6">
        <location>
            <begin position="390"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="281"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="348"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="290"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="302"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="349"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="368"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="380"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00054756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00072052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="548" mass="59416" checksum="1FFC2B900FD40B90" modified="2017-01-18" version="1">MADKSKDYSIQGYNLAKLLKRLEEATARLEDVTLFQEGYLLEKYGPKPAESTSTSSLNLPSKADGYTFSDADGSKSFEDPLVQSTTPASPALKTEANEDPKSIVEFKTFIANNVKPLVTISSKIDPVVEEAAKLYETAYLETLKFLTAAVKSSKPNVLSAEFAEALKPITAKMMAISDLKDKNRSSKFYSYLNSIAEGASVFSWITVTTPMSIISDFKEAAQFWTNKVLKEFKDTDPSSAEWVKTYLKGFDALKQYVKEYHTTGPSWKVAGGIPLSQALKEVTGEGSTESATSGNASGNASGPPPPPPPPPPASVFEVEKETSKAPESQGMNAVFASLNQGENITAGLKKVDKSKPLPDTTKKTAPIPSSKTPTGPIKPKKPENLKTKKPPKKEMSGSKWFIENFENTLNEPIVIEGNIDESVFIGKCKSVLIQVTGKVNAITVNDCADVNLVLDNSVSGVEFIKCNKFAVQVNGSCPQITIDKCDNGTIYLSKDSIEASICTSSSTSLNVNLPVGEDEDFVEFPVPEQMVHKYDSKKLKSTVYEHAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP40</accession>
    <name>A0A1E5RP40_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g962</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP40"/>
    <dbReference type="STRING" id="56408.A0A1E5RP40"/>
    <dbReference type="InParanoid" id="A0A1E5RP40"/>
    <dbReference type="OrthoDB" id="2162994at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051140">
        <property type="entry name" value="GATA_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="6">
        <location>
            <begin position="202"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="308"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="332" mass="37360" checksum="7BB246DD4EC77BC3" modified="2017-01-18" version="1">MPPDTDATLKFNLAQSPKLVLLLPDIKDPANGPLASNKPPTFKKSPRNSITLEMTRSELSVHSPDLRCRLLKRCSSDLGTQFSFKEQTTSVRSSHSCLVAPSSSHYTNTSFSSSSGSSGTLLRDKYSLSSSTSSLASIDRILSTSSPCRPEKIRPNYKSSKDELNSETEKHKCLNSKQNMLKLKVHKKNAPKPKQIRTHGLCLHCGPAATAEWRQGPYGLRSLCNSCGTYYNKLVNTIIALFNCSRNEAIRKANLVMYYMKYKLKRTDRESRKAIKALPYDAKWEDKVREVVPFKMDKDFFVITEKSHDKNGIRKVRTRNSKSSEDEISGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV01</accession>
    <name>A0A1E5RV01_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Multidrug resistance protein fnx1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g380</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RV01"/>
    <dbReference type="FunCoup" id="A0A1E5RV01">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RV01"/>
    <dbReference type="OrthoDB" id="3437016at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF191">
        <property type="entry name" value="VACUOLAR BASIC AMINO ACID TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="178"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="373"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="432"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="496"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="527"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="643"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="179"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="136"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ90760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="690" mass="76052" checksum="D9DE4F9F2356B213" modified="2017-01-18" version="1">MTPQKSGTPVSVDSSAASEINEPQVIPTNNVSQDISTVATISDKNGLQGAAKEPSVRSTPAQEEQAFSAPEALQEPDEADEITPLIANKDEYGLKSPNARPIMDPSLDFPGIVASEVQGLEDDYLERRSQLSMRRSRANSTYSSISKTTRTPYETSISKGEDKEMTNPHGLSTTRVRIILWSMYMGIFLASLDISIVSTLLTHIASEFNELGRISFIVTAYLLSNATFQPLYGKISDIFGRKSVFIFCNIMFSAGCYCCGVSNGLWGLVIGRFVSGIGGGGMTSMATIATSDLVSLRDRAYYQGIGNMFYGLGIAIGGLIGGQVNDWFGWRWAFLLQVPLSAVSCLAIVIYMNLPKPDASLYGSNMREKLGKIDWWGSLVLITFLALFILGSNVYIYLASFMFMLLFIYIELNIKSKDPILPVHFLKDRSVFGASFANWFCMMTSITLLYYLPIYWSTIIELSATETGKRLAPNFFSTGFGSLGSGIYLKRTGKYYNFLMGWSIFGVLGCLKIWLINPSVSVLQQYLMFVVPGFAMSVMITITLLVIIAAVPKEHQASCTSISYAFRSTGSTIGVSIGGAIFKKTVEKLLYKKVLPLESPEHSKKELMKIIETAMQNSDYVKKAPSFVKPVLKDCYNYALKNTFLFCLVAFILATLSVSMVREFKLHTSADRDDLIEEEEEGSNGEPAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVN7</accession>
    <name>A0A1E5RVN7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NAP1-binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g307</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVN7"/>
    <dbReference type="FunCoup" id="A0A1E5RVN7">
        <property type="interactions" value="184"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVN7"/>
    <dbReference type="InParanoid" id="A0A1E5RVN7"/>
    <dbReference type="OrthoDB" id="19092at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="156"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="111"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="271"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="277"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ90896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="304" mass="34757" checksum="2C3407B469E29900" modified="2017-01-18" version="1">MASTISVKDQIDPRACVLQAKEALQDSSHAENDRQLQGTTICEEDGLYQYEDEEMPPGCISVKDFAYTLEDPLHYGFYSDTEEYDEEAYGGESDEYDEHDPNSVLQEYYENDDDDEDEDNKTNHYQEHDQGNEYSQDKYFEDFDRRKSYIMPSEHIINRKAIALYDFENENENELGLQEGQVVFIDYRHGQGWLVVETMDGMHTGLVPEDYLQILDEGEEDEDADAEAHNGFANEYADATINNTVVNNTLDETHEDCAKRPDYLAGLFDVEQGTGPLPSNGNNYSSHTSLTDPLNEDLKTLSIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVP5</accession>
    <name>A0A1E5RVP5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g306</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVP5">
        <property type="interactions" value="147"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVP5"/>
    <dbReference type="InParanoid" id="A0A1E5RVP5"/>
    <dbReference type="OrthoDB" id="30179at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="175" mass="19746" checksum="5852DED394293194" modified="2017-01-18" version="1">MTSAHRSQLEARNGGKNNVKTNIKHSRLLPSHNTLKYRSDHKNKDLFEQGSYIEXDEQDTRKAEKLIESTNQEGSAANDPDLAQRKFSQEPTIXYSANGNKDQKXSNAESGISTSTSNLSHPLTNKSSWRKKAVFKKSPNKVNKQSHVGNFDSSSQGNTNPEKQKRQEFLKKVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVW5</accession>
    <name>A0A1E5RVW5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2415 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g292</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVW5">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RVW5"/>
    <dbReference type="OrthoDB" id="418169at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019417">
        <property type="entry name" value="DUF2415"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991:SF9">
        <property type="entry name" value="DUF2415 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991">
        <property type="entry name" value="WD REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_8G05640)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10313">
        <property type="entry name" value="DUF2415"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2415" evidence="2">
        <location>
            <begin position="382"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="431"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="564"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="599"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="441"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="565"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="599"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="620"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ90888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="772" mass="87728" checksum="7F598940C1766E7D" modified="2017-01-18" version="1">MTIEDAISYDSFFFSNDLKVFDTNVSINHWQLRDVVKGSSLVPYKINYVFQNKIKSFDSNIARSKFPHRGSRKIKPHHVQSDYNASTVCNNDVVLNYKPKCFQEIDEFYVXAGILESQNGVDFADLRPDELPETMEISNLSNFNTERGKWSSDWRGVLSFKHGDASATLRNMALDESRENRKVINVTLGKLINNDVALYSADRGYSGVSQCEMFVCNNDQNLYHLNYDLQNGNLQMLNVINDMHTPLNSCNLAHDGTKMIVSGDSNKFALYSHNINTKDINSKHKNDKSGTFFKISSTNNIVENLPQHELELDEISKINYSLNFEKTYTAEYGEYGFSSCFNESNTQFTSVFQNGYGLVYDVRRLDKPIKHLVSSRPCTQKGAFRVCKYSGDIDNLLFISENVGVVHVLDSKNFDNHYLVELPNIIHSQPPATVRTREPESSSINDENITYDQSSQLHSNESMSASYPLELGFGMQMSTSPRITTTSYDYETVDKMSANPNVNYLANAKESIQYQNFFKPHLTIFDNDNLFNSFKETFSTYYYYNTVFESSRARSTYRATLTDNDQIKEXNKSNSNEKNDPTGFKESLDLCHIHTLHPESKQSTEHFSHQKTDFDEKKPSKNNHTHKNRSRSRNLRFDTSDNPSLPEHMQFSQLLLRESGLGSNSPASLSPYGTNASSMSSFSTTSLSRYPLPIANIINFETQNALLNDPNDYTATLDPAYAQAKSKENDISGLDWVQDNEGTSLVIGTSLGIYKWGIKSSLRNSLSTYDFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RW75</accession>
    <name>A0A1E5RW75_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribonuclease MRP protein subunit RMP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g299</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RW75"/>
    <dbReference type="STRING" id="56408.A0A1E5RW75"/>
    <dbReference type="InParanoid" id="A0A1E5RW75"/>
    <dbReference type="OrthoDB" id="5414547at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000294">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, RNase MRP-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22573">
        <property type="entry name" value="RMP1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047205">
        <property type="entry name" value="RMP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047204">
        <property type="entry name" value="RMP1_RBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37792">
        <property type="entry name" value="RIBONUCLEASE MRP PROTEIN SUBUNIT RMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37792:SF1">
        <property type="entry name" value="RIBONUCLEASE MRP PROTEIN SUBUNIT RMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20945">
        <property type="entry name" value="RMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="93"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="RNase MRP protein 1 RNA binding" evidence="3">
        <location>
            <begin position="22"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ91018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="254" mass="29094" checksum="7BE98A2C36441F51" modified="2017-01-18" version="1">MDLQFEQDLEHYLRSLEEEYRIIAILNHRNRNQHRVAIWWKFFNILKRKTFDIIQRIHHILTSKITNVQKTLVRNVNQLYKSLHYFVKKQSKAVYYAFNGVIQLGQFVTLGVVLIGSFAKIQSNFEKLLQMLKKCKYLSINVIGKQGKLSNGANDRLVNTDDVHASNKEEIGEEVGEEVGEEVGEEVGEEVGEEVGEEVGQTLKVSVSKITFNKGQSLSVAETSKVTEPNTKVQPRKTKKTKRKSKNAIDDIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S031</accession>
    <name>A0A1E5S031_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Heme-responsive zinc finger transcription factor HAP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g109</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5S031">
        <property type="interactions" value="503"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S031"/>
    <dbReference type="InParanoid" id="A0A1E5S031"/>
    <dbReference type="OrthoDB" id="4159781at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051430">
        <property type="entry name" value="Fungal_TF_Env_Response"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31944">
        <property type="entry name" value="HEME-RESPONSIVE ZINC FINGER TRANSCRIPTION FACTOR HAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31944:SF131">
        <property type="entry name" value="HEME-RESPONSIVE ZINC FINGER TRANSCRIPTION FACTOR HAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="33"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="343"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="407"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="455"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="597"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="733"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1101"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1192"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="343"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="355"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="457"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="733"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="761"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1102"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1192"/>
            <end position="1204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ92484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1646" mass="181126" checksum="F9B7187FDE1B03C3" modified="2017-01-18" version="1">MDSTSTSSTVPPAISNHSHQDKPKRTRNRIALSCSICRARKVKCSKDKPHCKQCEKAGVAHLCTYLDQPWAAQSQKQITKDHELKILKERVKTLEEMLSKVHNSSTASLLNVLNSGNSNSRSNSSESNTSPANADFTNLINQNKNNSEMLLTSNNILANSSDTLINISGSKNGGNFTETNNIITPATSTAPNDANLKLMASPESGNDGSAVNSNNNGVDAVSKKYDYDELDLTKRFDMLHLKNTGTVHLGATHWLAIMKGDPYLKLLWSHIFTIREKITEWHKQQALFYNRKMAKRKCPHPLNIMEKAQCPVTHATGTVQNCPAEIISNAENERVQRQQIVPSSSADSVNNAMPQQQQQQQQQQHISQAXTVNTTAFNNQNDGTSTANPSKGKCPFDHTKLAGMKQPLAKPHKTPTGIPSPAATNPSIKNITHDTASTTFQSKCPVMHHDQLYPQPGQHSSYQQVDRPSSFGDLKKLGSPPLPNNMNPEQDFQIMQRVIDELNVLLPPQRVIALLIDKFFKFLYXVIPILDEQSFRSQIYQIIQAPQDKTKQNISIKIAKTLDYCTLGILVIILRLTWLSLPFNGCDTDLGLKIKNQDRQGKKGKSKTNSKGASATPSLTKDEELLRKYGISATAVSLVRKYLVRFDEISSFSNKNVNITTVQFAIFYKMYLSTCPEGSESSTSSASSSFTSTGHDNETHQVLLSSIVQMAFSCGLHRDPDNFPQLNSSDLISLPNTQHLNTKTGKTQNESKTSKKRKTNAENNPHTANSQINKESAANTEKLKHVWRKTWFFIVSLDVQQSLSLGTPRLLRNLGDFSDTKLPCSSKIDYVTDIKELIIVKNFTLFFQIDLCIIAVLNHILNVSLAKTVRKFELDELINALQVLSYGQNMEGELNANITEVVEKLISKGLLFTSEATALASTMSTMVPTAGAGLNSEDKEYTLPVLXKVLSTSTISSTIKKEKENGTSKKLDLPQEITTRALFFAKHLTIRMLLYLLNYILFTHYEPQGNEDIGTLPLTAHYAQSALNFAMDGFKNSVLFFVTNKQTSAKSSNCGESIFSHMEVLLAPQCLDACHRSLQFMICLILRARCGPLTGLNETAITSTSGSNSGSGMSSEEENDNSSRTKAKKDYDSMLFDKNEKLELNDDLGELLLSRMELFHRLTRKVSVKYKYAEKMTRSTGFFLTLLKDTSKKSGSSKKDRTNDEAATSAANAEPHGHGMEFASGATTAGNVTEMPSFMLPKLKXTGAGNMSKFFKNIPSLIISAGGDQLKRCPVYQDAVGFFPKDLHQGAFSKVLSNPSAPRNPTNNFVLPHITTPNSSAQAVSPYQPVTYSAISRRTSDIRNEELNEMHKRRKISAPNLLMPSPEPPSITPRQRMLSETLPLPGLRSVAASPLPAAGSIVKTENMSPSQVRPDNFVGFPSPLLRNDNQEAVAAAPQINQPIPQMNPTNMPFNPVNKLLVANQYLQENGQHVSQPLTSSPSLMSEATSSSSVNYAPDFEEFLKQSTDINGLIVNPSNFMEAVGFDDFRDPSNGLLNSGLRSGSMAGLATHGXNSIVGGVNGPSDINFSRAGMVSDLDFMDDARQFSEANSVWVINEDFQGMESTGIEGLHEIINGNSATTLAAGHNTEINVGHNQPGVPDFSSWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5QZZ2</accession>
    <name>A0A1E5QZZ2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Signaling mucin MSB2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g4649</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000017">
        <property type="protein sequence ID" value="OEJ80229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5QZZ2"/>
    <dbReference type="InParanoid" id="A0A1E5QZZ2"/>
    <dbReference type="OrthoDB" id="3366093at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005034">
        <property type="term" value="F:osmosensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000282">
        <property type="term" value="P:cellular bud site selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006972">
        <property type="term" value="P:hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007232">
        <property type="term" value="P:osmosensory signaling pathway via Sho1 osmosensor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001402">
        <property type="term" value="P:signal transduction involved in filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039295">
        <property type="entry name" value="MSB2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35778">
        <property type="entry name" value="SIGNALING MUCIN HKR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35778:SF1">
        <property type="entry name" value="SIGNALING MUCIN HKR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184542" evidence="3">
        <location>
            <begin position="20"/>
            <end position="1542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1176"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="505"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="714"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="844"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1146"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1240"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1457"/>
            <end position="1497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="183"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="225"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="307"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="515"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="627"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="714"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="730"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1251"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1457"/>
            <end position="1488"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="4">
        <location>
            <position position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1542" mass="164353" checksum="FDB400B9EE8BB0A1" modified="2017-01-18" version="1">MNMLFAFIXLALLSSFVQSIYLSNGEEQRTNTKKKRPKFYNRAETLFPSPYANTSSFISPDTTNNYLTFSIPSSSSSGYSSQNEANSFPFLDAFFVTSSVSVASATTSSSSQSLQFTTSATGDFSNTNYITTSAASGTTESQTXNSYESSNTNXFSTAFSGSSTLAFISSSFAGSSNFVSSTPISISDSSQQTNSATKTSLLSSTPETDLVPTSETAFSSTRDITSSPTSETTSXPXPXXXXSPTXETTSXPTPETTSSPTPETTSSPTPETTSSPSPESTYAPNPEASFTNXRNHFFTNSRXNLLTNSSASPXPEXTSSPTPEXTSSXTPETTSSPXXETNTAVVPITTYDVDTFTSIPITEPFSSGSLTEFTESSDDYEYWTFSTNWIDYTGTGNEWPGLIVDTAGSSIFTFDLGSSFSXSSALSTSKDYTGIQLKTSSPTFKSTSASKVSPTSXLXSLTTVPSLSPSSSTMIDVTTSLSFESDISPTTSETLITSVFTTSSSVKATGPSKLTETSITESSTMEXSTVLSSTAETTSSPIPETTSSPASETTSSPXPETTSSPTPETTSSPTPETTSSPTLEPTSSPTPETTYSPTPETTSSPIPETTSSPTPETTSSPIPEATYSSTQEITSSPIPGATYASITEASTVLSTSFEGITSNQLSKISSTLGPEPAVTNTFSSLSFSTTSTSAFYTSIKSSESSTMFYLTTSTSPNSPFTSSNPEIPIETSNEKSTTTQNEPVXTESGDFTTLVPSSAISASISVTAFVSEQAVSDTTGSTLTLSSLSVTTDTRGVETTLSEGTAVSQATTLLETRASRATTTAPSSGPVSINSAQSSAVTTTLANAHSSTESPLGVGQNTDATTETSFSYASRGSGEITGITTPSSLVDFSQQISGATAATASALATTSYWLPYSLVTTYIVTKPSASNLHTTAXQTAIATSLPQAIYPQSSVSVPVDYTVIGIAFEEAFNYEYLLNSYIAAAQIINFLPELLKYPFLHSDYQKREVVNKGSLKAASNFEYVAVVEIVPYYDSSYDYILSVAKVSFPTASVTHLQALISNRKSSLYTVADKTLSSLAALINSDFKIMSDTWLSSALQSGYTVTTQSVKQTITTDYVTVSNGTPITVYTTFQTYVPVTATIANSQRGPHVTDNSNGSLDQYHSSTSMNKRTRNRFIIYITCLSXGFLLIVFVLFFLLQGIFSITKIQKLLSHDLERGHSIPQELLLPVLWPAFSSDLTGSEPDTDRGSHYTENSNGGHSQLNEKETECSSLESANFNTTRGSLVTEGTLRYFVDQDGNYYYAGESTGEEDASDNFSGHEDSDSPFYDEGINSEEDISIPELPSVEVESVTHDDIEVDEDGNVLIDMGTEFDMTDQPGFMDPSSELTPERLEKLLQEKLLQNSGVSADGEQDPMGTKSLNELLNLSDSGPTTDEIIANSIINDQELHEFLYSDMGSDSYGNSSSNNNSSNNTTPNYSSNSMSNYFSSNSEERTSDVSDVNVGEIDTLDAEIYLRIKPYYEKALPGLVRQVRTRSSTNANDTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0K4</accession>
    <name>A0A1E5R0K4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R0K4"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="129"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="160" mass="18114" checksum="6A0799738F23CEA8" modified="2017-01-18" version="1">MYENKNNEICHVGQYEIYNRNSGHPGTMCHRDYGSISVSXVETKCDAMEIDDAPLASTNGTSCGFFDAPCSTNVHYLSILKEYPECEPYMLDLLEKNRLSGPSSSNLNSCSSIQQQQQQQQQQQQQQQQQQQQRQQQPQQYTANSSSIQGLSSSLHHLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0V9</accession>
    <name>A0A1E5R0V9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">thioredoxin-dependent peroxiredoxin</fullName>
            <ecNumber evidence="1">1.11.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Thioredoxin peroxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g4526</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peroxiredoxin family. BCP/PrxQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0V9"/>
    <dbReference type="FunCoup" id="A0A1E5R0V9">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0V9"/>
    <dbReference type="InParanoid" id="A0A1E5R0V9"/>
    <dbReference type="OrthoDB" id="338622at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03017">
        <property type="entry name" value="PRX_BCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050924">
        <property type="entry name" value="Peroxiredoxin_BCP/PrxQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42801:SF23">
        <property type="entry name" value="PEROXIREDOXIN DOT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42801">
        <property type="entry name" value="THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="11">
        <location>
            <begin position="110"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="24"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="64"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ80517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="273" mass="30981" checksum="3366C7824EDCF3EE" modified="2017-01-18" version="1">MSEARKSERLANLKKPNSGHPEYENQSIRNPAVNEQSITHNTDSNKENGANTASTSGPAHKKIKVNDEHLKEKVAETDFEAQKKSELSRDETKDRKTQESKEAAKDEPSEEIQKTAPKIDSLEIPDDLELQLENEKTVNVKKDVINDGKLKLIFAYPKACTSGCTKQAQGFKKIYNELEKTYEGRVEIYGLSADKPAENLKFKQELDLPFHLLSDPERKLIHLLKASNKDNTKTVRSHFVWDNNGKLIHENIQVSPDDSIEKGENQLTNFLRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0W4</accession>
    <name>A0A1E5R0W4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lysophosphatidic acid:oleoyl-CoA acyltransferase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4589</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0W4">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0W4"/>
    <dbReference type="InParanoid" id="A0A1E5R0W4"/>
    <dbReference type="OrthoDB" id="272512at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="350" mass="40023" checksum="68FB92DA30EFDE53" modified="2017-01-18" version="1">MKRDSRRFPQTWLVSWKIVCLIKDVQKPYTSVPSKVRMEKYGEYRDSGTGIAPFLPIVLSASAKGGVTTKIFRYAKLVVKLIIALPVLLVYQFIPLQMFLNVLFKILIGFSTNVQVEGLKKTKIEVQRDYPQKNQIYYCNYTNPLDYWCLSQISRGNKFVVLVAHXKNQVIEFNDNSWWTFALFTLVTTGILPPKATGKGRGFQIVQLDKSLAQKYKDKIVYCFPEGTTSNGKSILPFELDCKNLENHVKFNTVNTISMRISPSHLVTPLPYEDMDSTKYWWKLLLFSSDGNGLXXGGSSGVECKYKISHKVNSSDVRIKLNNDDKYKLIGQSLKKDKKAKFIKVYKGPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R176</accession>
    <name>A0A1E5R176_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Saccharopine dehydrogenase [NADP(+), L-glutamate-forming]</fullName>
            <ecNumber evidence="8">1.5.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Saccharopine reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4309</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-saccharopine + NADP(+) + H2O = (S)-2-amino-6-oxohexanoate + L-glutamate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10020"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57951"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the saccharopine dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.10" evidence="8"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R176">
        <property type="interactions" value="193"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R176"/>
    <dbReference type="InParanoid" id="A0A1E5R176"/>
    <dbReference type="OrthoDB" id="10059875at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004755">
        <property type="term" value="F:saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000008">
        <property type="entry name" value="Alpha-aminoadipic semialdehyde synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000072">
        <property type="entry name" value="Saccharopine dehydrogenase [NADP(+), L-glutamate-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1870.10:FF:000002">
        <property type="entry name" value="Saccharopine dehydrogenase Lys9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1870.10">
        <property type="entry name" value="Domain 3, Saccharopine reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051168">
        <property type="entry name" value="AASS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032095">
        <property type="entry name" value="Sacchrp_dh-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133:SF22">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16653">
        <property type="entry name" value="Sacchrp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="11">
        <location>
            <begin position="5"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Saccharopine dehydrogenase-like C-terminal" evidence="12">
        <location>
            <begin position="123"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ80646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="447" mass="49383" checksum="DB116960E7EDA8ED" modified="2017-01-18" version="1">MVKRVLLLGSGFVAQPVVDVLAKTEGIEVTVACRTLPKAQELAASSKSNAISLDVTKDDEFDAALAQHDVVISLIPYIYHPNVVRAAIRLKKDVITSSYISPALRELEPEIKKAGITVMNEIGLDPGIDHLYAVKTIDEVHKAGGKIKSFLSYCGGLPAPEDSDNPLGYKFSWSSRGVLLALRNSAKYWENGKIVNVKSEDLMSXAKPYFIYPGYAFVCYPNRDSTVFKDLYHIPEAETVIRGTLRFQGFPEFVKALVDMGMLKDEANPIFESSETSWKSALQQYLNAKSDSKTDLIAKIDELTSWKDEADRERILNGFSWLGMFSDDKIIARGNPLDTLCATLEKLMQYEEGERDMVVLQHKFGIEWADGSKETRTATLVDYGKVGGYSSMAATVGLPVAIATKMVLDGEIKGPGLVAPYSPSINDPIMKELKEKYGIYLIEKTIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1K1</accession>
    <name>A0A1E5R1K1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GDP-mannose transporter</fullName>
            <shortName evidence="5">GMT</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g4345</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the import of GDP-mannose from the cytoplasm into the Golgi lumen.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homooligomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Golgi apparatus membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasmic vesicle membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TPT transporter family. SLC35D subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1K1"/>
    <dbReference type="FunCoup" id="A0A1E5R1K1">
        <property type="interactions" value="540"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1K1"/>
    <dbReference type="InParanoid" id="A0A1E5R1K1"/>
    <dbReference type="OrthoDB" id="417037at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00803">
        <property type="entry name" value="nst"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="186"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ80785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="338" mass="36919" checksum="F230B59A8397E0E9" modified="2017-01-18" version="1">MSELRVEKPAGGVSNIVNNGPVSIMAYCFSSILMTVTNKFVVNLDNFNMNFVMLFVQCTVCTLLLVVLKLLGYAKFRSLNKTDAKNWLPISVLLVLMIYTSSKALQFLPVPIYTIFKNLTIILIAYGEVLFFGGSVSTMELGSFLLMVFSSVIATWGDQQEIHKKAAAAAAAAAGETLNSSLFNAGYVWMFTNCISSALFVLIMRKRIKVTNFKDYDTMFYNNILALPILLGASFLVEDWSKENLAVNLSKDSLSAMIISGMSSVGISYCSGWCVRVTSSTTYSMVGALNKLPIALSGLIFFDAPKNFLSISSIFVGFLSGLVYAVAKQKKTQNSAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1N7</accession>
    <name>A0A1E5R1N7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS10m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">37S ribosomal protein S10, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4246</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1N7"/>
    <dbReference type="FunCoup" id="A0A1E5R1N7">
        <property type="interactions" value="278"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1N7"/>
    <dbReference type="InParanoid" id="A0A1E5R1N7"/>
    <dbReference type="OrthoDB" id="366214at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.600:FF:000003">
        <property type="entry name" value="30S ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="entry name" value="Ribosomal protein S10 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00508">
        <property type="entry name" value="Ribosomal_uS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_uS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_uS10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_uS10_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01049">
        <property type="entry name" value="rpsJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S10 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00971">
        <property type="entry name" value="RIBOSOMALS10"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="Ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS10" evidence="6">
        <location>
            <begin position="94"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="256" mass="29146" checksum="6B25547C3A8576F7" modified="2017-01-18" version="1">MQKFVAKSLGNATSGCRLLTSAKASSKLVVLSQRYNSSAAQGAIPQENQNEISTFNTSISKVATEPKQDVPANVKAIYYQPLRNPVQYGDLVCDLQLRAYDNENLDFFSDFILRAGFYLGAALTGPKPLPTKRERWTVIKAPFVFAKTKENFERRTHKRLIRVWDTNPEVVDLLMSYITKHSITGVGLKVNFYQKQGLDLNFEQEFQKLENTIGTEQAYQDLDEKNDHISKRVGEILNDPVFKQYMKPEDKPSLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1T9</accession>
    <name>A0A1E5R1T9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">GPI mannosyltransferase 1</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">GPI mannosyltransferase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g3863</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the first alpha-1,4-mannose to GlcN-acyl-PI during GPI precursor assembly. Required for cell wall integrity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Endoplasmic reticulum membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the PIGM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1T9">
        <property type="interactions" value="593"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1T9"/>
    <dbReference type="InParanoid" id="A0A1E5R1T9"/>
    <dbReference type="OrthoDB" id="1741594at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990529">
        <property type="term" value="C:glycosylphosphatidylinositol-mannosyltransferase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051751">
        <property type="term" value="F:alpha-1,4-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004376">
        <property type="term" value="F:GPI mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007704">
        <property type="entry name" value="PIG-M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12886:SF0">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12886">
        <property type="entry name" value="PIG-M MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05007">
        <property type="entry name" value="Mannosyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 13 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 13" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="145"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="277"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="360"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="389"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ80852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="436" mass="50248" checksum="B1D31E6CC031D882" modified="2017-01-18" version="1">MSMYQDSHFTVKYTDIDYFVFNDAARFVYNGDRSPYLRDTYRYTPLLSYILLPNFYFNNIHLGKFXFVLFDILTGVLIFKLILLVQNRXTYKDXSKTLADKHKAKTPADGASVETSTNRAALLSALWLLNPMVITISTRGNAESVLCAMIMASLYCLAKXQYILGGLLLGLSIHFKLYPVIYAIPIVIYFFAKYGFQSKAVIAVGKIGTSCVLSLLGLTYSMYQKYGYEFLFHSYIYHFVRTDHRHNFSVWNMLLYLESSPAYKKLFPHGVPTSSKWATIPQMLMSSVLIPSMLCFSKYNALNNXKRSLNFLDLTNVIFAQTFAFVIFNKVITSQYFIWYLVYLPMFLFKCGGTTTMKKNAAIVVMWVLAQAVWLYYGYQLEFKGQNVFYPYLFGAHLVFFTGNVWVLGQIIEMIKKSEYVEASVDKTALMEKKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1X8</accession>
    <name>A0A1E5R1X8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 9">Uracil-DNA glycosylase</fullName>
            <shortName evidence="7">UDG</shortName>
            <ecNumber evidence="7 9">3.2.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">UNG1</name>
        <name evidence="12" type="ORF">AWRI3579_g4177</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 9">
            <text>Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.</text>
            <dbReference type="EC" id="3.2.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 9">Belongs to the uracil-DNA glycosylase (UDG) superfamily. UNG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.27" evidence="7 9"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1X8">
        <property type="interactions" value="315"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1X8"/>
    <dbReference type="InParanoid" id="A0A1E5R1X8"/>
    <dbReference type="OrthoDB" id="10031947at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097510">
        <property type="term" value="P:base-excision repair, AP site formation via deaminated base removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10027">
        <property type="entry name" value="UDG-F1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.470.10:FF:000007">
        <property type="entry name" value="Uracil-DNA glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00148">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002043">
        <property type="entry name" value="UDG_fam1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018085">
        <property type="entry name" value="Ura-DNA_Glyclase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003588">
        <property type="entry name" value="PRK05254.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003589">
        <property type="entry name" value="PRK05254.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003592">
        <property type="entry name" value="PRK05254.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00628">
        <property type="entry name" value="ung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11264">
        <property type="entry name" value="URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11264:SF0">
        <property type="entry name" value="URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00987">
        <property type="entry name" value="UreE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00130">
        <property type="entry name" value="U_DNA_GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5 7 9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="11">
        <location>
            <begin position="136"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="30"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7 8">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ80906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="343" mass="39265" checksum="7CDD4AE292449894" modified="2017-01-18" version="1">MSITDSESNALQSTRKRKTIDTFFKPAKXSKKDEQSQQDSVDKAAAKVTADLTLLKKPIISEHDTXPNNXLSGPVDDETASLLDNELKTIEPSWFCMLKDELTKPYFLELKKYLRSEALNRKQIFPSPENIYSWTRLTPFDDVRVVIIGQDPYHNVNQAHGLAFSVLKPTVAPPSLKNIYKELQTHNYSDFVIDKNCGDLSKWSKQGVLLLNTCLTVRAHEAFSHSKRGWEVFTKKIIELLINDRLQKKDKPICFMLWGSKAQDLVYKNFKNIDFDHAKNFLMLKSVHPSPLSASRGFFGGKQFKQCNEWLAERFHQPMIDWSVVDGTSLQXVQAFNSQLSTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R259</accession>
    <name>A0A1E5R259_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Guanylate kinase</fullName>
            <ecNumber evidence="2">2.7.4.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">GMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g4159</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the guanylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.8" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R259">
        <property type="interactions" value="570"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R259"/>
    <dbReference type="InParanoid" id="A0A1E5R259"/>
    <dbReference type="OrthoDB" id="6334211at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004385">
        <property type="term" value="F:GMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000002">
        <property type="entry name" value="Guanylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000776">
        <property type="entry name" value="Guanylate kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.10">
        <property type="entry name" value="Guanylate Kinase phosphate binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017665">
        <property type="entry name" value="Guanylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03263">
        <property type="entry name" value="guanyl_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23117:SF13">
        <property type="entry name" value="GUANYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23117">
        <property type="entry name" value="GUANYLATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Guanylate kinase-like" evidence="9">
        <location>
            <begin position="2"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="185" mass="20538" checksum="6CAA9FF7FF1CA274" modified="2017-01-18" version="1">MSRPIVLSGPSGTGKSTLLKKLFAEYPNSFGFSVSSTTRSPRAGEVNGKDYNFVTVDEFKKMIDEKKFVEWAQFSGNYYGTTIKSVKQVAETGKTCILDIDMQGVKAVKLTDLNGRFLFISPPSVESLRXRLTGRGTETEESLNKRLAAATAEMEYAESGAHDKIIVNDDLEKAYAELKEFIFXK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2L6</accession>
    <name>A0A1E5R2L6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2L6"/>
    <dbReference type="InParanoid" id="A0A1E5R2L6"/>
    <dbReference type="OrthoDB" id="8059989at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="1">
        <location>
            <begin position="128"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="791" mass="89006" checksum="72E87914A59D09FB" modified="2017-01-18" version="1">MTESXKVLSKNPEHHCTNFGAKGHNYNXLTSIKIPAALRTTIYSCVNPVLSIPIHSSMPSLVSSETTTQCDDTGSLTEKYLSQESLARQDIYYRNSAPGGHISNLDLKTSSLTSIDQMSIKYTKIIAKRNYILSELVSTEKVFVSXLNILEKEYLDKLVGCTNGLYESLKAMXCYTRELRKXHSQILAKVKXLLETQYDXSINPLVLCADIAEFLSSKVIAVYYYQNYIHNYYITLAALQKVGSKDKKFSKELVNKVDSGSLALEKQYKKFLLQDLAAKKGLVKADASXNSEIFGVIKNXQTSNXRSLYEGFQILQKEMFASSGANASLKVDLSFKSLIQSPVSRISKYRIFLQQILALFCGDVXVAMCLKSQEXFVKIEKGLSVLSKKLEEINNFDNTAAPSNEALHKEPLHSFISTHLPXFTLSGRXRGAXLPIETLGQCHLSGPCIALWITKQSDYCPQCATEKTNKNHVKVTVIYSKKDRSAGCTXYKKYAXRHSTRATLTGKASYLFLFNSHLLIAQPYTDQDQLTVLFSIPLHTSXIXSGNKLXKLLXENPESLRGLHTDYEHRFKIQFKIKNKNYELVLAFYNKIEHCXWEXKLLLLIDEAQDSCLDRLTMSSAQNSCLKKMPPNYLCTVVPKNICPMTMLKDRLTTKGCLKSWFHLFKCXGANELSKPVFFTILFTRGKALEXWELPSRDVSPHVSSASISSSANSASISKNFSSVVCVDLQTRLFMQQKFKSIWNHDFPXLTSAPSILGNQFESKQCCPINELXESIGTWELPSLKSHSSRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2M0</accession>
    <name>A0A1E5R2M0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Endopolyphosphatase</fullName>
            <ecNumber evidence="3 12">3.6.1.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g4163</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the hydrolysis of inorganic polyphosphate (polyP) chains of many hundreds of phosphate residues into shorter lengths.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>[phosphate](n+1) + n H2O = (n+1) phosphate + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:22452"/>
            <dbReference type="Rhea" id="RHEA-COMP:14280"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16838"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the endopolyphosphatase PPN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.10" evidence="3 12"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2M0"/>
    <dbReference type="FunCoup" id="A0A1E5R2M0">
        <property type="interactions" value="252"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2M0"/>
    <dbReference type="InParanoid" id="A0A1E5R2M0"/>
    <dbReference type="OrthoDB" id="348678at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000298">
        <property type="term" value="F:endopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004309">
        <property type="term" value="F:exopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006798">
        <property type="term" value="P:polyphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00842">
        <property type="entry name" value="MPP_ASMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041805">
        <property type="entry name" value="ASMase/PPN1_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012358">
        <property type="entry name" value="EndopolyPtase_N1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340:SF55">
        <property type="entry name" value="ENDOPOLYPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027093">
        <property type="entry name" value="EndopolyPtase_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 12" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="105"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="555"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="64"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="134"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="556"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="575"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="15">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR027093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ80813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="679" mass="79053" checksum="CB7C7B628F466693" modified="2017-01-18" version="1">MREKAYQNDETEKYVSQNTNISDEFHADIHKNSSKRKLKLLLTFFLILVLGATYFSKQQALTHSSNSPAVASSQSSELDGKTQRRAKKLTGRFIHITDIHPDPHYVEGSSIESSCHRKESSSSALDEGEEEVYKKKKKTPKKQTKAHKFGDAMQGCDSSMELMQESMKWISQEFPASEIDFIVWTGDNIRHDNDRRIPRTQTEIVEMNKNMSDMFHELWAVPETEGPFLPETIPIIPSLGNNDVFPHNVVDIGPGQFMRTFNNIWASFIPEEQQRSFDRFMSFFQEIIPGKLAVLSINTLHFYKSNPLVDNCNDKDQAGYQMLIWFGLLMQEFRDRGMKVWITGHVPPIEKNFESSCYQKFNLWCWEYRDLIVGGLYGHMNMDHFVPINGRDLGERLRGANLENMDLDLQSRQDTVGNTDMSSDAFSINNEVTIFGAKPVNKMKYIKTLKQQYKHVSKLQNKQNSDTFDPADYLVINVAGSVIPTFNPAFRVWEYNITGLYDNDEDAQEYDQLNEYLPWAEFFTQVDEQIDTIVHEDYENFGKDTLFTKLKESIMKKGKKKNKKNKKKKKKQKKDKSIPPKMPKDAALGPAYKPQLFSPVSFKQYYLDLPRFNKNLKQSPKDTVFEYDLEYTFNNTQMESLLVDGYLKYCKSLIKDKQLWKKYLKWLFVSTDYENLEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2N6</accession>
    <name>A0A1E5R2N6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">SED5-binding protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g4126</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC23/SEC24 family. SEC24 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2N6">
        <property type="interactions" value="166"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2N6"/>
    <dbReference type="InParanoid" id="A0A1E5R2N6"/>
    <dbReference type="OrthoDB" id="49016at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050550">
        <property type="entry name" value="SEC23_SEC24_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803">
        <property type="entry name" value="SEC24-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803:SF4">
        <property type="entry name" value="SECRETORY 24CD, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="7">
        <location>
            <begin position="237"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="8">
        <location>
            <begin position="320"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="10">
        <location>
            <begin position="575"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="9">
        <location>
            <begin position="673"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="973"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="29"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="973"/>
            <end position="984"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ80833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="992" mass="110993" checksum="BE5470E88011966A" modified="2017-01-18" version="1">MDNLASNVADMNIGDAPSRKKKPARAYHQFSQPQSNFAYAGNTSSPSPLLATGNFSPMPQVGQAMNSSTPTLGNVPQYPGTPQGYANGQNLGFPSAEINVSSIHTGVAQTVDLATQRLLDQKEYLSKTFDTSRDSILPLKTTQFYSVDTGSCDXRLMGLTMYNIPKNETLRNMTKLPMGVHVQPFAAITALGKEPQQNQTAYGDSQDGMDAAFTPEAEQESTQESLYVNEINPQIGIIRCKRCRAYNNHNFKFSGDQSSFVCNFCLMKSSVGSLQSSNSGMFGGAPLGSGEPNFIAPPFAGVLDFKAPPEYNNIPNVESKPLHYVFLVDISTFANGNKSSVSCIDAIQTSIEYIAKNQPFCKICIIGFDKNLHFFKLNKDQQTAQEFIVNDLYGDTRHSGNESGSVFLPFYDGLFVSADESMHVIEDTLNRLKTFCLQTNTGYVKHETDVCFGPALEAAMMALSTFAQGGKILCSLNALPKFSRGSLQLRKDDAFVKNLKFGDNDYYKKLGYQLLKSNCGVDLFITTDGFMDICNVGFPSMITGGQLKYYPHFQAARDDFQVSSDYLNSVSNTIGYQGSLKLRCSSGLSVDTYYKESCNFSNQEPHFAILTKETNVSILLKYDGNTVNSKEQKDFYFQTAVLYTNLKGERRIRVLNTSGAIQSNLFEIYKFVDQENVANIMIKDICLTLMKNQATDYSVVRKTIDNKLVDVFXQYRAVTNNHSVNNNMVLPESLKLLPTMMLAFESSKLMKQAKQSTRANERVLQLFEFMNAPLSKLFFKLYPQIIPLHVMMNQELDLVLYDLNKKLLSIDSTDEFAVSNSGRKLIDGGCYLIFTGDLAYFWYNQNTNPLLLKDLLGYDNTSKSNDGRPLPIEIECGLFPKVDTEINRKVSALLTYWKKLCYFDNYIKVVPLSPLNSQASVSAGLGGSSFPNILSYEILFDDKNIENIESLENYLILLHKGIKEKMDKQDYITPSKKDQQKEHAFQQWYQQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4K6</accession>
    <name>A0A1E5R4K6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nucleolar protein 16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3690</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biogenesis of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NOP16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4K6">
        <property type="interactions" value="321"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4K6"/>
    <dbReference type="InParanoid" id="A0A1E5R4K6"/>
    <dbReference type="OrthoDB" id="285729at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019002">
        <property type="entry name" value="Ribosome_biogenesis_Nop16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13243">
        <property type="entry name" value="HSPC111 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13243:SF1">
        <property type="entry name" value="NUCLEOLAR PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09420">
        <property type="entry name" value="Nop16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ81845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="236" mass="26908" checksum="DC59D8CA3717F5BF" modified="2017-01-18" version="1">MARSSVKKATRRTKDKQREANIRGNAIIAENWDYSQTLAQNYKRLGLKAKLGSAAGGQEADLSSKIMKNSLVRAQTIADLDSDDEDVDDGTASADYINPEELNSDGEFDEKKIPAGEARIKRDSSGKVVKVIYGTKKFDIDQSVEVLKKEQQSTNAQKDESKTQVVQQLEEYANRPIVRQERVESGREDEWLEKLYKKHGSNYKKMFFDVKLNIYQQSEGDLKRRVEKWKARKGXN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R518</accession>
    <name>A0A1E5R518_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">adenosine kinase</fullName>
            <ecNumber evidence="4">2.7.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3543</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.20" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R518"/>
    <dbReference type="FunCoup" id="A0A1E5R518">
        <property type="interactions" value="1262"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R518"/>
    <dbReference type="InParanoid" id="A0A1E5R518"/>
    <dbReference type="OrthoDB" id="432447at2759"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00659"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004001">
        <property type="term" value="F:adenosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01168">
        <property type="entry name" value="adenosine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001805">
        <property type="entry name" value="Adenokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45769">
        <property type="entry name" value="ADENOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45769:SF3">
        <property type="entry name" value="ADENOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00989">
        <property type="entry name" value="ADENOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="11">
        <location>
            <begin position="97"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601805-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ81989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="429" mass="47031" checksum="07FB7F516E68F361" modified="2017-01-18" version="1">MLQYTTRSISFLKLDRFPRASTLNILCNSPIFRFNSHHFLNSKTESPTFRFQNNIFESLKTNTTAKPYKMAQSQSPELVCLGNPLLDIQVDTNAEFLAKYNLKSNDAILIEDSSKDPRMEIFQELIKNDKSLYIAGGAAQNTARGAAYILGKGKVGYFGCVGEDQFSEQLLKENEKAGVVSMYQIDQTHGTGKCAALITGIDRSLVTDLGAANHFKPEHLDKHWDVVEKAKAFYIGGFHLTVSPEAIIKLGKHAQETNKPFVLNFSAPFIPQFFKSQLESVLPYATHVIANESEAESFAESFALDIKDKTNLEAIATEILKLFPYKNTNGEVANKTIIFTHGTEPTVAVTSSSSSSSSSSSSIEVPVHPLEASKIKDTNGAGDAFASGFMAGIVLGDSLQKSIDRGQWLAKLSIQEVGASFPSEKIAYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R671</accession>
    <name>A0A1E5R671_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetyltransferase SLI1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3639</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R671"/>
    <dbReference type="FunCoup" id="A0A1E5R671">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R671"/>
    <dbReference type="InParanoid" id="A0A1E5R671"/>
    <dbReference type="OrthoDB" id="3972366at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052058">
        <property type="entry name" value="Alcohol_O-acetyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010828">
        <property type="entry name" value="Atf2/Sli1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28037">
        <property type="entry name" value="ALCOHOL O-ACETYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28037:SF1">
        <property type="entry name" value="ALCOHOL O-ACETYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07247">
        <property type="entry name" value="AATase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ82033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="497" mass="56164" checksum="D1581EE884B2567E" modified="2017-01-18" version="1">MSPTEIPKGEMSFWAKYFYTKVCLEMGTCVYVGVQYTKLPELPELYAALKRLVDENPPLGLNIFAAGVEDMNCNIVQTPNGPVKPDPPQNLHAAQLKEVDFSTVVENLTNFEIHDFETVKYILQRKFEYGSFTKPLWKVTLLKDNWLIFSYDHAFIDGMSGANIQKKLVVILNDMNKNNGSYSGIDSTYKITSGSPDDNFGIINIYEKPENTWGFATRNLLDSKIYPVLKSSAKALVQKIGLNPTWLGRFNEFECNAKYVVQKNRFIDNFQNVSITAKDVEKLIGIGKKHGNVGFNSLMGALLSVSAKNTFVLEEQSVPFMFLVSERLKRKLDPESIGILIKAEEETTPILSNALFDSVGLNHTEFWKFASQVHADMTRAIDTGNGLEHFNMVGLQDLVTKVKAQEGSSPHFLFCFSNLSLQFKEKRFKEGNGLKYAIKDAVFNQSQVYADYFTVSSIATPKGGFNMHMVYQDEVKKETEMLLANFEKLYKELINSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R688</accession>
    <name>A0A1E5R688_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g3771</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R688">
        <property type="interactions" value="319"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R688"/>
    <dbReference type="InParanoid" id="A0A1E5R688"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01093">
        <property type="entry name" value="CRIB_PAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06614">
        <property type="entry name" value="STKc_PAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000139">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.810.10:FF:000005">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033923">
        <property type="entry name" value="PAK_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF22">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SAMKA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PH" evidence="11">
        <location>
            <begin position="180"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="CRIB" evidence="13">
        <location>
            <begin position="357"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="825"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="64"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="287"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="446"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="516"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="64"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="96"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="109"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="292"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="446"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="466"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="533"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="588"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="651"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="674"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="698"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="774"/>
            <end position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ82053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1125" mass="123272" checksum="7961E1385BBDD7A7" modified="2017-01-18" version="1">MSFSAAARDISDADFQNIGPAPKPPGVSFNKTQPLAKAKSNMELNKKFTSQFLKNTNSSKTTLATTPAAVTAGSNSRTSGDSLSSGSSTSSSTFSKNGNAGGNNNMSTSYGNSSRNMNSNNYGSSSHSSGGLSNSSKNNMKKNNDMDANSSYKNYTNNSASHNNNDSKISNPNAVLKHLKQQKKGWVSYKDDGILSFLWQKRYMVLGESYLILYKNDSRFEEPVLKIPLTSILNVSRNQLKQNCFEIIRSLDRNSFGYSGSSSAAIANNSEAKNILSNNYSSISNQALYGTNNPNSSXNNNNNNNNNNNNNSNNSNNNNNNNNTSTKKTIYIATKSEIDTNTWLDAIFVKCPLLSGVSTPTNFTHKVHVGFDPETGNFVGMPSNWEKLLKHSRITGEDWNNDSAAVIQVLQFYQDYNNGTTGFNPASADTRTAPSSSNTALNQLKTVAGNSRSASSDTLTNKRVGSNNNNTAATSHSNISTNNNFRIPSIPKLGLQNTTGLPKNPDASRENLLFENSDLIPKRKAPSPPGPKTLQNTNKLGPNNANGVSTPNKSFSNTTGSGPSKPLNPLHIDTAKINDSKSFSKVNTPTSATYRTPTTSTPTKSSFGTLDPSKQQTLGSGGPSVAQHAAGVPTNKTGVYPLSTQQLQQQARLHSQNPNNGSRTPKSRLPNVQVPXNQMIQQPQSMRNTSQGDHRQHSNTNSPYSQPLLSGSQTSVHKQQQSPTTRIPPLRMQPQRAAPPPPKQEGVATSMLPGMKAPQNKPPPHQAGSQPQTQKAPAQDQQSKQDAKPQPPAKQVKKKAPQMSNAELMAKLKTVCFDTDPSQYFKVVEKAGQGASGSVYLAKRVNMPPFDKSVVRRSKFYEATPPMLSDKVAIKQMILSKQPRKELIVNEILIMKDSKHKNIVNFLEAYLKNEDTLWVVMEYMQGGSLTDIIENSPHAGTSTPALAEPQIAYIVRETCQGLRFLHERHIIHRDIKSDNVLLDTTGRVKITDFGFCARLTDQRRKRATMVGTPYWMAPEVVKQRDYDEKVDVWSLGIMTIEMLEGDPPYLNDEEPLKVLFLIATNGTPKLKYPETLSLEIKRFLSVCLCVDVKYRASTEELLHHSFFDKACSPEDLAPLLEVRPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7H1</accession>
    <name>A0A1E5R7H1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA polymerase II transcription factor B subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3362</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the TFB2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7H1"/>
    <dbReference type="FunCoup" id="A0A1E5R7H1">
        <property type="interactions" value="504"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7H1"/>
    <dbReference type="InParanoid" id="A0A1E5R7H1"/>
    <dbReference type="OrthoDB" id="364513at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2610:FF:000001">
        <property type="entry name" value="General transcription factor IIH subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040662">
        <property type="entry name" value="Tfb2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004598">
        <property type="entry name" value="TFIIH_p52/Tfb2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00625">
        <property type="entry name" value="tfb2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13152:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13152">
        <property type="entry name" value="TFIIH, POLYPEPTIDE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03849">
        <property type="entry name" value="Tfb2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18307">
        <property type="entry name" value="Tfb2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor Tfb2 C-terminal" evidence="10">
        <location>
            <begin position="459"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ82859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="537" mass="61424" checksum="117E412537A5A5FF" modified="2017-01-18" version="1">MSTNNLFKTTVLEYLEGLPQNVQSRLYESPASCLAIYRILPQLAKFLIMSMVFNEQEVALTDLSAWVTHQGKYEFNEAIKTMSSLHVIIKAEDPNDRADTKISLNHVFRISFRNALTGGEVHKSFGVVIEDDTDDIVLDPKADQSTLEKLRKLKQAKKRKNRAKSTQFLDQYAADKWETILHYMVGTPGMKAPSETVLNLLIHGNLMKEEQNGDLKITNEGFQFLLQEVNVQIWALLLQYLKLAESLQMNPVDVLNFIFMLGSLELGQRYSIDSLSKVQLSLLKDMSNYGLIYHDEQAKEFYSTRMATILTSDLMSIRSASNAMNSVIAHKDSDESAAAKQLMNQEDELDPSNIMDGALIIETNFKLYSYSNSPLQIAILSLFVHLKARFSNMVTGQITRESIRRALKNGITAEQIIAYLESHAHPQMIRMAEEQLTKKTELDPNTTETLQILPPTVVDQIKLWQLELDRIIHYDGYLYSDFDTLKEYHELANYADDIGVLIWKNDKKKMFFVDYEGNSQVVEYAKRLVKREDQDAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7J5</accession>
    <name>A0A1E5R7J5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 17">Homoaconitase, mitochondrial</fullName>
            <ecNumber evidence="5 17">4.2.1.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16 17">Homoaconitate hydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AWRI3579_g3217</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 17">Catalyzes the reversible hydration of cis-homoaconitate to (2R,3S)-homoisocitrate, a step in the alpha-aminoadipate pathway for lysine biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 17">
            <text>(2R,3S)-homoisocitrate = cis-homoaconitate + H2O</text>
            <dbReference type="Rhea" id="RHEA:15485"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15404"/>
            <dbReference type="ChEBI" id="CHEBI:58174"/>
            <dbReference type="EC" id="4.2.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="17">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-alpha-aminoadipate from 2-oxoglutarate: step 3/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.36" evidence="5 17"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7J5"/>
    <dbReference type="FunCoup" id="A0A1E5R7J5">
        <property type="interactions" value="148"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7J5"/>
    <dbReference type="InParanoid" id="A0A1E5R7J5"/>
    <dbReference type="OrthoDB" id="10262323at2759"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER01027"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004409">
        <property type="term" value="F:homoaconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01674">
        <property type="entry name" value="Homoaconitase_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004418">
        <property type="entry name" value="Homoaconitase_mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039386">
        <property type="entry name" value="Homoaconitase_swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050067">
        <property type="entry name" value="IPM_dehydratase_rel_enz"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00139">
        <property type="entry name" value="h_aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822:SF2">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 17" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 17" id="KW-0408">Iron</keyword>
    <keyword evidence="11 17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="14 17" id="KW-0456">Lyase</keyword>
    <keyword evidence="13 17" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="8 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 17" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="18">
        <location>
            <begin position="44"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="19">
        <location>
            <begin position="503"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OEJ82866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="705" mass="77482" checksum="8A7DCA3CF6FBD8E9" modified="2017-01-18" version="1">MLRSRVSLYSKRLLHTTRAQYYQNLTEKIAQKYAIPATSDVEYHTGDYITVKPYHCMTHDNSWPVALKFMNIGGKKIYDREQVILTIDHDVQNKSESNLTKYKNIENFAAKHNLQFYKPGAGIGHQIMIENGYAAPNTLTVASDSHSNTYGGIGCLGTPIVRTDAACIWATGQFWWQIPPVAKVELIGTLPEGVSGKDVIVGLCGVFNKDQVLNHCIEFTGSGVKSLPVDQRLTIANMTTEWGALSGLFPVDETLINWYKDQIKSKESNLYKTLKRQNRDPQEFIKELEYEAQNFTADSKAEYAKTLTIDLSSLTHYVSGPNSVKVAQPISKLADMKINKAYLVSCTNSRLSDLQSAAKVMKGHKVYPGVEMYIAAASKTIEEQAKKDGTWETLVEAGCKPLPSGCGPCIGLGAGLLEPGEVGISATNRNFKGRMGSKDALAFLASPAVVAASAIKGHMAAPNSIADEHFTSIKAEILANKESNKQETTKEKQEELKIEGNQESAPKVEILEGFPRNITGELVLCDQDNINTDGIYPGKYTYQDNIDKETMARVCMENYDSQFNTKVNKGDILVSGFNFGTGSSREQAATALLAKGVPLVVAGSFSNIFSRNSINNALLTLEIPKLVELLRKERAASESSSKELTWRTNYFLTWDVANSQVTVQKKGDPQPILQTKVGVLGKNLQEIIVKGGLEGWVMSKTAAEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7K7</accession>
    <name>A0A1E5R7K7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent RNA helicase DED1</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ATP-dependent RNA helicase ded1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AWRI3579_g3361</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">ATP-binding RNA helicase involved in translation initiation. Remodels RNA in response to ADP and ATP concentrations by facilitating disruption, but also formation of RNA duplexes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7K7">
        <property type="interactions" value="1392"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7K7"/>
    <dbReference type="InParanoid" id="A0A1E5R7K7"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17967">
        <property type="entry name" value="DEADc_DDX3_DDX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000397">
        <property type="entry name" value="Probable ATP-dependent RNA helicase DDX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044763">
        <property type="entry name" value="Ded1/Dbp1_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DEAD_ATP_HELICASE"/>
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    </dbReference>
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        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HELICASE_CTER"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 15 20" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="19">
        <location>
            <begin position="169"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="200"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="401"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="30"/>
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    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="561"/>
            <end position="648"/>
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    <feature type="short sequence motif" description="Q motif" evidence="14">
        <location>
            <begin position="169"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="565"/>
            <end position="576"/>
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        <location>
            <begin position="585"/>
            <end position="611"/>
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            <begin position="626"/>
            <end position="648"/>
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    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00012552"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
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            <dbReference type="ARBA" id="ARBA00022917"/>
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            <dbReference type="ARBA" id="ARBA00024358"/>
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            <dbReference type="ARBA" id="ARBA00024397"/>
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            <dbReference type="ARBA" id="ARBA00024405"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
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    </evidence>
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    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51195"/>
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    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OEJ82861.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="648" mass="70650" checksum="7A8671C804576F32" modified="2017-01-18" version="1">MVYRPPNKRNLDSANPSDPNGSAPSYNNTXGGGSFFRSYNTGFNGGSRGGYGGGGSRGGYGNGGSRGGYGGGYGGGGSRGGYGNGGGRGGFGRSNNNFYDTKSSQIQKNFTSGRWKDGQHVPAPRNEQYEIKLFGVPEDPSFQSKGINFDHYDDIESEATGHDVPEQITEFTSPPINKLLLENIELARFTKPTPVQKYSIPIISAKRDLMACAQTGSGKTGGFLFPVLSECFDNGPLPTPANPNREYYTKPCYPTALILAPTRELASQIFDEAKKFCYRSWVKPCVIYGGASAEPQISNMSKGCDLLVATPGRLIDMLARGTVKLCNIRYLVLDEADRMLDMGFEPQIRQIVEESDMPDSTARQTLMFSATFPVDIQQLAHDFLNDYIFCTIGRVGSTSENITQKFLYVEEHDKQGALLDILENXENGSQMTLVFVQTKRMADALTDFLIISGYKATAIHGDRSQAERERALAAFKRGVANLLVATAVAARGLDIPNVKHVINFDLPTDVDDYVHRIGRTGRAGNTGVATSFFSYENQKLAPDLIELLEEAKQEVPVFMKNMVQGNRRGNRTGNNTRDFRKQPGAHSNSRQSNFGASNWGNARSSQASSWGNAPPRRNNDGASSWGNNSRANTSSNNASSSNGSNSWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7Q0</accession>
    <name>A0A1E5R7Q0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="14">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">AWRI3579_g3363</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 24">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="14"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7Q0"/>
    <dbReference type="FunCoup" id="A0A1E5R7Q0">
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    </dbReference>
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    <dbReference type="InParanoid" id="A0A1E5R7Q0"/>
    <dbReference type="OrthoDB" id="10248617at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
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    </dbReference>
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        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
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        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
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        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
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        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007646">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007647">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007175">
        <property type="entry name" value="PRK09606.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04566">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04567">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
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    </dbReference>
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        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14 22" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="18">
        <location>
            <begin position="53"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="17">
        <location>
            <begin position="199"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="19">
        <location>
            <begin position="454"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="20">
        <location>
            <begin position="552"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="21">
        <location>
            <begin position="634"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="15">
        <location>
            <begin position="681"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="16">
        <location>
            <begin position="1052"/>
            <end position="1137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00053978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF04566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF04567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="OEJ82924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1143" mass="128600" checksum="3AFDBF4EE88627DC" modified="2017-01-18" version="1">MTDIVTDHSHHHIKDESFDALMKPVYKGKGLTDEINTAEDKWNLLPAFLKVKGLVKQHLDSFNYFVDVDLKKIINANKVILSDVDPEFYLKYVDIRIGHRSNSSSQDKIVPPHQCRLRDMTYSAPIYVDVEYTRGRKIIMHKDVEIGKMPIMLRSNKCILNGQNETDMARLNECPLDPGGYFIVNGTEKVILVQEQLSKNRIIVEADDKKHIVQASVTSSTHERKSKTYVVTKHDKIYLKHNSISEEVPIVIVLKACGIISDLEIVQLVCGNDSTYQELFAVNFEEAAKYRIYTQRQALEFIGSKVKTIRRQKLTVLQEGIEAIATTVIAHLTAENLDFREKALYVALMTRRVVMAMNDPKMVDDRDYVGNKRLELAGQLMSLLFEDLFKKFNSDFKASIDKVLKKPNRATEYDALLSINIHSNNITSGLNRAISTGNWSLKRFKMERAGVTHVLSRLSYISALGMMTRISSQFEKSRKVSGPRALQPSQFGMLCTSDTPEGEACGLVKNLALMTHITSDDEEEPIKRLCYLLGIEDLSLIDSSSLHLHYGVYLNGTFIGITRFPIKFVSQFRKLRRSGKISEFISIYTNQHQNAVHIATDGGRICRPLIIVTNGESKVKAEHLLQLKNGQLQFDDFLKLGLVEYLDVNEENDSFIALYEKDISNEITHLEIEPFTVLGAVAGLIPYPHHNQSPRNTYQCAMGKQAIGAIAYNQFKRIDTLLYLMIYPQQPMVKTKTIELIDYDKLPAGQNATVAVMSYSGYDIEDALVINKSSIDRGFGRCETRRKTTCVLKRYPNHTQDIIGGMRVDENGKPIWQHAPLGPDGLGEVGMKVESGQVYINKSVPSNASDSVLSQQSSQYRETPVVYRAPEASHIDQVMMSVSDNDQALIKVLLRQNRRPELGDKFSSRHGQKGVCGIIVQQEDFPFNDQGVCPDIIMNPHGFPSRMTVGKMIELVSGKAGTLNGTLEYGTCFGGSKLEDMSKILVDHGYNYSGKDMLYSGVTGECLQAYIFFGPIYYQKLKHMVLDKMHARARGPRAVLTRQPTEGRSRDGGLRLGEMERDCVIAYGASQLLLERLMISSDAFQVDVCNACGLMGYGGWCTTCKSSENVIKMTIPYAAKLLFQELLSMNIAPRLKLDDVFSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7T5</accession>
    <name>A0A1E5R7T5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14">Mevalonate kinase</fullName>
            <shortName evidence="14">MK</shortName>
            <ecNumber evidence="3 14">2.7.1.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g3464</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Mevalonate kinase; part of the second module of ergosterol biosynthesis pathway that includes the middle steps of the pathway. The second module is carried out in the vacuole and involves the formation of farnesyl diphosphate, which is also an important intermediate in the biosynthesis of ubiquinone, dolichol, heme and prenylated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(R)-mevalonate + ATP = (R)-5-phosphomevalonate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17065"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.36"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:17066"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="13 14">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the GHMP kinase family. Mevalonate kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.36" evidence="3 14"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7T5"/>
    <dbReference type="FunCoup" id="A0A1E5R7T5">
        <property type="interactions" value="431"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7T5"/>
    <dbReference type="InParanoid" id="A0A1E5R7T5"/>
    <dbReference type="OrthoDB" id="1652964at2759"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00098"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004496">
        <property type="term" value="F:mevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006205">
        <property type="entry name" value="Mev_gal_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00549">
        <property type="entry name" value="mevalon_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290:SF2">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 14 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11 14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="14" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="14" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="15">
        <location>
            <begin position="153"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="16">
        <location>
            <begin position="334"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ82979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="461" mass="50436" checksum="98E7EE39050F2836" modified="2017-01-18" version="1">MPNIQMTQDSAFSPFVATAPGKVIIFGEHSAVYGQPAIAAAVSTLRTYLLVVPTKRQDTHNSEIVELSFPDIKFDHEWTKEDLQECANYASKLISNASQTAQKSIENMDLDDTLAKMIGELLHKTLPDCTNQLHHAAALCFLYLYFIICPKLTNAKFILKSNLPIGAGLGSSASISVCLSLAFMKLSNDARFQNIHTLNSEVKDLVNKFSLIGEKCIHGTPSGIDNAVATYGNAVLFQKLLDPSEQQRAGVTNKMEILNEFPEIPMILTNTKIPKSTKTLVSNVNILFQKNPLIFDPIFQAMGHITNKGHETLTNIYKLNDENGNVKKKTKLMNDLLELVKVNHGLLTSMNVSHPGLEKIVNLSTELQVGATKLTGAGGGGCALTILNDVGNETNGKEALENMALFEKTLIDDYKYDIYRTGLGGKGAYLLPSLDQSIIEEFQSADGLPSSILNYINSKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7U8</accession>
    <name>A0A1E5R7U8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3461</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7U8"/>
    <dbReference type="FunCoup" id="A0A1E5R7U8">
        <property type="interactions" value="301"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7U8"/>
    <dbReference type="InParanoid" id="A0A1E5R7U8"/>
    <dbReference type="OrthoDB" id="3972684at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF137">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE HRK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="391"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="304"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="346"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="652"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="34"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="48"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="132"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="194"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="229"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="271"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="309"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="652"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="731"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="753"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ82951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="891" mass="100220" checksum="98E7131F16189B40" modified="2017-01-18" version="1">MFKLFKHKDSEKHKDKGSKSNNSSSNSLGSFHNKKNDDDRMNHDRTTTKSTNTSTTKSTNTSTTKSTNTSTNKSTNTSTNNISNSSNRSRRSSTTNLNNINNNINNKSNDVHDINKTKDNKYHIENSDNVSTTTSETSGHTTTNDTTATTATTTTNNNNNNNTANNHNNHNNNTTNDNIINNSNTTINDNNNNHIKDYHNPKDYHNATSNVSNLLGSLTPSASTDREPHHHSHNHHKGKSFFGISLSKHNSNNSQMSSIHSPITSNEDNSHSSYSGHSNNNHNNNHNSSMVEIKRFFRPQMLTKDHHTNNNNNNNNNNNNNNSHHHIGMNKRPSFSSIASFTRTDSTHHNQHHNHYTHTQTAIPPTNESSLSLSNKSNMYHDDTLLVQKYGKLGKILGSGAGGSVRIIKRPSDGVVFAVKEFRPKKPDEPMKEYVKKCTAEFCIGSTLHHPNIVETLDIFQDVANCKFWEVMEYCPVDFFSVVMSGKMSRNEINCCFKQLFCGVEYLHSKMGLAHRDLKLDNCCMTPDGILKIIDFGSAVVFKYPYDKNITLAKGVVGSDPYLAPEVMAPQARYDPQPVDVWSCGIIYCCMVLKRFPWKSPNKYKDKNFHLFCLDDEIEHDYVESARKHAELLANMKREKIKQKQETLRLKEIKAEDENKETTKESEEKNDEKKDGVDKEDEDKTQNGVSEAKKSAEQQPEIKPDHVDVDLPSKHPAQAPESTVAASTAEPVKDEVEQKEEKKAEEEGHSNAPAPSNANETAPAPSTNNETAPQESKEQAPSASASTAKPVAKSGPRKPLQGPYRLFRLLPHASRPIMSRILEVDPTKRASMKEIFEDEWFQSISRCTLDARKQLIRGKGHIHTLVFEHDGETTITTNVAYQQEHQKEHEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7Y7</accession>
    <name>A0A1E5R7Y7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Cap-associated protein CAF20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3469</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Acts as an inhibitor of cap-dependent translation. Competes with eIF4G1 and EAP1 for binding to eIF4E and interferes with the formation of the eIF4F complex, inhibiting translation and stabilizing mRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the CAF20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7Y7"/>
    <dbReference type="FunCoup" id="A0A1E5R7Y7">
        <property type="interactions" value="367"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7Y7"/>
    <dbReference type="InParanoid" id="A0A1E5R7Y7"/>
    <dbReference type="OrthoDB" id="3995390at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008190">
        <property type="term" value="F:eukaryotic initiation factor 4E binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031456">
        <property type="entry name" value="Caf20"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17052">
        <property type="entry name" value="CAF20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-0652">Protein synthesis inhibitor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0810">Translation regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="50"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ83004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="156" mass="17656" checksum="03D94337D352F361" modified="2017-01-18" version="1">MAVIRYTEEELLELQPLAVVPKDFDPKEFNDLVSKVSEILALREEEYEKHHGFGNRRRSSHHFNAKPKVHKHKPKVTTDADGWSSLDSEKPEKTSFAFSETSNNNGSDSKKLETVKVRPNNKNISSSRPADSRDIIADKNTVAFNAFAALESDEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R840</accession>
    <name>A0A1E5R840_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ATP-dependent permease PDR12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g3287</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R840">
        <property type="interactions" value="215"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R840"/>
    <dbReference type="InParanoid" id="A0A1E5R840"/>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000054">
        <property type="entry name" value="ABC multidrug transporter atrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="534"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="566"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="620"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="678"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1204"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1233"/>
            <end position="1255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1276"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1315"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1348"/>
            <end position="1367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1470"/>
            <end position="1488"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="170"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="863"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1512"/>
            <end position="1535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1512"/>
            <end position="1529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ83075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1535" mass="173822" checksum="EAC72EF77C8A3B3E" modified="2017-01-18" version="1">MSSSGSSIKEEKAPSTEDDNAPKPLSQTHKPEGDDVSSYAGSIQSYENGPEQTHPEGERDVESQNIESQLSRHVTNILSETNGAERIESLARVLSTKTKKQLEHFEIREGIDFDLQQLLDYLRSHQLDQGIVPGDAGVAFKDLTAVGIDASAAVAPSVGEMVRGWVHHPLKMFNRKKTDLVLRNIIQNFTSVIESGEMMLVIARPGAGASTLLKCIGGETSELVEVTGEFSYDGLDQHDMMSRFKGYVIYCPELDFHFPKITVKETIDFALRCKTPRVRIDGMSRKEYVDSMRDLWCTVFGLRHTYATNVGNDQVRGVSGGERKRVSLVEAIAMNGSIICWDNATRGLDASTALEFAQAIRTATNMTNTAAIVCIYQAGQNIFELFDKVTVIYNGKQIYMGPAEEGVEYFTNMGWVKPNRMTSPEFLTSVTLDYDNRVLDVKPGFEASIPKNSDEFLDYWVNSPQYAKNLGQYDEYVANHNAEETRQRLAESIEQRKQAREPLNSLYVVNYWHQVWYCMIRGVQRVKGDTTYTIVYLSSFLTKGFFVGSMFWRIDPIDQSTTEGAYSRGGLIFYVLLFCSLTSLAEISNSFANRGIIVKHKTYSMYHTSAEALQEIFTEMPVKFVAVLVLCLISYWLPALKWQAGSFFQYLLYMFTVQQCTSFIFKLCATLTKDGGTAHAVGGLWVLMLSLAASFFLPIGQMHHWIRWFHYINPLTYAFESVMSTEFHGRQMLCSKLIPSGTNYENVSIENQVCQATGAVKGQRYVSGDTYLKKAYHFEYKHAWRDWGINVVWTFGYIIMNVIMSEYLKPLEGGGDLLLYKRGFMPSFGDNADAKVASREEMMEALNGPNVDLDEVIASKDVFTWNNLTYTIPYDGSTRVLLNSVFGYVKPGKMTALMGESGAGKTTLLNTLAQRIRMGTITGDMLVNNKPLPASFKRSCGYVAQADNHMAELTVRESLKFAAELRQPASVPLDEKMDYVEKIIRLLGMQNYAEATVGKIGRGLNVEQRKKLSIGVELVAKPSLLLFLDEPTSGLDSQSAWSIVQFMRALADSGQSILCTIHQPSATLFEQFDRLLLLKKGGFMVYFGDIGENSNALTGYFERQSGIKCGVSENPAEYMLNRIGAGATANASSDWHELWENSPECAAGRQELAELHEKLNARPVTEDKELSSRFAVSLFTQFKCVLWRTNIQFWRSPVYIRAKFLECILCALYVGLSYVGTGVDYGVQGAQSSFASTFMMLLIALAMVNQMHVFAYDSRELYEVREADSNTFHWSALLICHTWLEIAWSSICVFICFICYYWPGQFSGRASHAGYWFLIYVIMFPIYFISYGSWIIYMAPDVPSASMINSNLFAMLLLFCGILQPFHKMPGFWTFMYKVSPFTYVVQSLVSSIVHNKKVICNPHEFAILDPPQGQTCEQFLAFYRDENKGYIDNPDATDNCRYCPYTVSDQIVEQYGIRWDQRWRNFGLLWAYIVFNFVAMCVCYYIMRVKVWSVKGILDIKKWYHGPRKERHEPETNVFKEKAHDKKNVTKSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R8M7</accession>
    <name>A0A1E5R8M7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Arginase</fullName>
            <ecNumber evidence="2 10">3.5.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3347</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>L-arginine + H2O = urea + L-ornithine</text>
            <dbReference type="Rhea" id="RHEA:20569"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="EC" id="3.5.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="10">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the arginase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.3.1" evidence="2 10"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R8M7"/>
    <dbReference type="FunCoup" id="A0A1E5R8M7">
        <property type="interactions" value="1331"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R8M7"/>
    <dbReference type="InParanoid" id="A0A1E5R8M7"/>
    <dbReference type="OrthoDB" id="9992747at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004053">
        <property type="term" value="F:arginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006525">
        <property type="term" value="P:arginine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09989">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.10:FF:000012">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014033">
        <property type="entry name" value="Arginase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01229">
        <property type="entry name" value="rocF_arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43782">
        <property type="entry name" value="ARGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43782:SF3">
        <property type="entry name" value="ARGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00116">
        <property type="entry name" value="ARGINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0056">Arginine metabolism</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ82883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="323" mass="35464" checksum="C32925BD6B751936" modified="2017-01-18" version="1">MATAQPHYKHLKEKSVSIVLAPFSGGQGKGGVENGPDYMLKHGLEKQLQELGWKTSIKEPLKDTNYEELKQTATEKSATSKTKRGDLVGEATKKIYNTCKNEIGDDFQLTIGGDHSIAMGSIAGLLQRYPDAGIIWIDAHADINTVTTTESGNLHGCPVSFLMGLDKENYPPELQWIPNQVLKTDKICYIGLRDVDAGEKKILQENNIKAFSMYHVDRFGINSVVEMAIDHVSKGNKDCPLMISYDVDALEPSYVKCTGTMVKNGLTLREGFFICERIAETGNLVALDVVEVNPELGTNDMDILDTIMAGCAVVRCTMGETLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R905</accession>
    <name>A0A1E5R905_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Mannosyl phosphorylinositol ceramide synthase CSH1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3266</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R905"/>
    <dbReference type="FunCoup" id="A0A1E5R905">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R905"/>
    <dbReference type="InParanoid" id="A0A1E5R905"/>
    <dbReference type="OrthoDB" id="3647at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000030">
        <property type="term" value="F:mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051999">
        <property type="term" value="P:mannosyl-inositol phosphorylceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051706">
        <property type="entry name" value="Glycosyltransferase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32385">
        <property type="entry name" value="MANNOSYL PHOSPHORYLINOSITOL CERAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32385:SF20">
        <property type="entry name" value="MANNOSYL PHOSPHORYLINOSITOL CERAMIDE SYNTHASE CSH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="234"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="281"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="322"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="322"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ83013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="395" mass="45939" checksum="BED3ACD3C21514CE" modified="2017-01-18" version="1">MIVDDPLTNAITDEELSDTYNDSSPQLIPKIIHQTYKTKDIPEHWKAGQQACIDLHPDYEYKLWTDDIMEEFMEKEYPWFLETYLSYGENIQRADAIRYFILYHYGGIYIDLDDGCQRKLDPLLRYPSFFRKTSPLGISNDVIGSAQGHPLMLKLTKKLKGYNINYFNVPYWTIMASTGPMFVSMIWEQYKRNDGGKDYKILQPHQYKNSLGSFFKISEGSSWHSSDSNFIFKMGAHILGCVVFGFIVFFIIFYTEYIFYKSLQNFSLQECANSINAMGQFFIRLVKSLVKFVVLYVINPLKHVLHLDSVFASSTYQQLQQQSQGMDQSSSSKNNTNNNNNNNNIINNNKNSKNKNASTDQQLQQQYKRLRKDSNLPVRLNLLLDLEKNGDIENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R9Z0</accession>
    <name>A0A1E5R9Z0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial import inner membrane translocase subunit TIM16</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Mitochondrial import inner membrane translocase subunit tim16</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12 13">Presequence translocated-associated motor subunit PAM16</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g3131</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. In the complex, it is required to regulate activity of mtHSP70 (SSC1) via its interaction with PAM18/TIM14. May act by positioning PAM18/TIM14 in juxtaposition to mtHSP70 at the translocon to maximize ATPase stimulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TIM16/PAM16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R9Z0">
        <property type="interactions" value="247"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R9Z0"/>
    <dbReference type="InParanoid" id="A0A1E5R9Z0"/>
    <dbReference type="OrthoDB" id="10262892at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000006">
        <property type="entry name" value="Import inner membrane translocase subunit TIM16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005341">
        <property type="entry name" value="Tim16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12388">
        <property type="entry name" value="MITOCHONDRIA ASSOCIATED GRANULOCYTE MACROPHAGE CSF SIGNALING MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12388:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03656">
        <property type="entry name" value="Pam16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="117"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ83729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="145" mass="15520" checksum="D62AC7D07BE1C018" modified="2017-01-18" version="1">MSYRAFFKVVFTGAEIVGKAFSEAYKQTAAQSAKGAASGAKAGGAAKEIGGITVXESCQILNLQKADINNLDKINKQYEYLFNVNDVEKGGSFYLQSKIYRAAERLKHDIKQAQXELQVAEDEAAGKKPATSTSSHPEAAAGPHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA31</accession>
    <name>A0A1E5RA31_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Casein kinase II subunit alpha</fullName>
            <shortName evidence="11">CK II alpha</shortName>
            <ecNumber evidence="11">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g2547</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Phosphorylates YTA7 during S-phase to promote transcription of histones.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="11"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RA31"/>
    <dbReference type="FunCoup" id="A0A1E5RA31">
        <property type="interactions" value="841"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA31"/>
    <dbReference type="InParanoid" id="A0A1E5RA31"/>
    <dbReference type="OrthoDB" id="10254671at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032545">
        <property type="term" value="C:CURI complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007535">
        <property type="term" value="P:donor selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060962">
        <property type="term" value="P:regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006356">
        <property type="term" value="P:regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006359">
        <property type="term" value="P:regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14132">
        <property type="entry name" value="STKc_CK2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000459">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000088">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045216">
        <property type="entry name" value="CK2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054:SF27">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="52"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ83766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="342" mass="40359" checksum="E174B9BA6D8DE747" modified="2017-01-18" version="1">MSAELQPTILNKPAAHVYSVARVYTHACTDKPREYWDYEQCTQLKFGDITRYEILCKIGRGKYSEVFRGCFADEFKKLCVIKVLKPVKMKKIYRELKILTHLVGGTNVITLYDIVQDQECKVPALIFENVGNVDYKPLYGSFSLMDIQHYFRQLLYALDYCHSMGIMHRDVKPQNVMIDPIKRELRLIDWGLAEFYHPGVDYNIRVASRYHKGPELLVNLKQYDYSLDLWSVGCMIGGIIFNKDPLFKGDSNPDQLIKITQILGTKELLQYLQHYKLPLPSEYDKILKNYERKPWEKLITDRNRRRCIPELVDLVDKLVRYDHQERLTAKEALDHEFFHMEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA65</accession>
    <name>A0A1E5RA65_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ATG1-like MIT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2833</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RA65"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048941">
        <property type="entry name" value="ATG1-like_MIT2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21127">
        <property type="entry name" value="ATG1-like_MIT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATG1-like MIT" evidence="2">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ83800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="87" mass="9421" checksum="529EEF7360BCFDCC" modified="2017-01-18" version="1">MLESLLDEEPASDDGKYGNXANTVNEEVGGETDKVNTQSNTATDIQSTSKLDSNDQEMIKKYITGIARRLKSLKASTTASQANISLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA93</accession>
    <name>A0A1E5RA93_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thioredoxin reductase</fullName>
            <ecNumber evidence="8">1.8.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2719</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[thioredoxin]-dithiol + NADP(+) = [thioredoxin]-disulfide + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="9">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.9" evidence="8"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RA93"/>
    <dbReference type="FunCoup" id="A0A1E5RA93">
        <property type="interactions" value="257"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA93"/>
    <dbReference type="InParanoid" id="A0A1E5RA93"/>
    <dbReference type="OrthoDB" id="371245at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000064">
        <property type="entry name" value="Thioredoxin reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050097">
        <property type="entry name" value="Ferredoxin-NADP_redctase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008255">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_2_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005982">
        <property type="entry name" value="Thioredox_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01292">
        <property type="entry name" value="TRX_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48105">
        <property type="entry name" value="THIOREDOXIN REDUCTASE 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00573">
        <property type="entry name" value="PYRIDINE_REDOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 8" id="KW-0274">FAD</keyword>
    <keyword evidence="2 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 9" id="KW-0521">NADP</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 8" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="10">
        <location>
            <begin position="5"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ83807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="319" mass="34410" checksum="7287AE7D1A8A0B50" modified="2017-01-18" version="1">MVHQRVTIIGSGPAAHTAAIYLARAEIKPTLYEGMFANGIAAGGQLTTTTEIENFPGFPEGLTGSELMDRMKEQSVKFGTDIITETVAKLDLSSKPFKFWTEFNEDEEPNTTDAVILATGASAKRLNLPGEETYWQQGISACAVCDGAVPIFRNKPLAVVGGGDSACEEAGFLTKYGSKVYLIVRKDHLRASTIMAKRAEKNPKIEIIYNTVTLEAQGDGKLLQSLKLKNTKENKEYELPVNGLFYAIGHNPATDIVKGQVDLHEDGYVKTVPGTSMTSLPGVFAAGDVQDSKYRQAITSAGSGCMAGLDAERYISELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAA2</accession>
    <name>A0A1E5RAA2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome production factor 2 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Ribosome biogenesis protein RPF2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2517</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
            <location evidence="1 4">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the RPF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAA2"/>
    <dbReference type="FunCoup" id="A0A1E5RAA2">
        <property type="interactions" value="1042"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAA2"/>
    <dbReference type="InParanoid" id="A0A1E5RAA2"/>
    <dbReference type="OrthoDB" id="407658at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039770">
        <property type="entry name" value="Rpf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12728">
        <property type="entry name" value="BRIX DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12728:SF0">
        <property type="entry name" value="RIBOSOME PRODUCTION FACTOR 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Brix" evidence="6">
        <location>
            <begin position="28"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="321"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="321"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ83824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="340" mass="39052" checksum="1A3AFCA22D8FB3A0" modified="2017-01-18" version="1">MIRTVKPKNARSKRALEKKESKLVENVKQALFIPGNKTSKVLHDAMVDLSALKKPYCKRFTRKNQVLPFEDASTLEFFSEKNDSSIMVLGTHSKKRPNNLTFARTFGFKLYDMIELQIVNNYKLLEDFKKITFNVGLKPMFSFQGAIFDTHPVYKHVKSLFMDLYRGEVADLQDVAGLQHVISISVEREFDETVSNNLPNVLFRVYKLKTYKSDVGGKKLPRVELEEIGPRFDFKIGRIQLPSQDMVKEAYKKPKEPHGSGNKNIALPKNVELDPMGDKLGRIHVGKQDLNKLQTRKMKGLKSKYDQNIGDEDDMEAYMNEEEDLIDAEDAEPSTKKQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAC7</accession>
    <name>A0A1E5RAC7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Altered inheritance of mitochondria protein 9, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Found in mitochondrial proteome protein 29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2923</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AIM9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAC7">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAC7"/>
    <dbReference type="InParanoid" id="A0A1E5RAC7"/>
    <dbReference type="OrthoDB" id="2968323at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051035">
        <property type="entry name" value="Mito_inheritance_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36091">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36091:SF1">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ83847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="622" mass="71241" checksum="1606050883430986" modified="2017-01-18" version="1">MIRSARIGSKQALRPVSKYYGSVVSHSVAVKNLHTTSKKQIAKEPEEVFTKLSDENDPNRESFFNYTWGSWLKNDAEEKKKRVTKFSLEGLTKVLNDIYKQSAALGISDAVQPPSYNXNLTVTLPHNLSSKFIGEGPXTEIKIKTMSSIHEGKHHRIYKLDTDNGKSFILRVPYPKETEVAIAERLKSEVATQDFADLQLKIKVPKIFCFGVNSMNPVRAPFILQEFIEGDLLMKDWDPLVDDSALEGKPHEEKLNKVIKPLADYQAKLASVEFSKHGSLYFAKDVSQDKSXESHASSTEDLFTDESKSAYKGRWAVGPTQERGFWRSKDLLTKEVHSEYLGPWNSSSKILSDLAELELANSKAKLNKNTESTILQNQVKTFENLTKLAPALFNEKSKNIPNVEKLFKPRLQHPDLDPMNVIVNEKGEKYLIDFEGSVIKPFMYNIQPQFVAYEGPKLYKFDQESEEFKKLNDQEKEQYKFMYKRTRNLYLWEFALNSTAPELIYSVAPQMKLLKAPIIATLNKRADEDYIFVDEKMIQLKSIWELMAKNQLVNAETFPLTYTDKDIEDHTAVMMKNNEELIKSPFAVTQGWIPADMFENLVQQQILIKQENGDYEFNASGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAH0</accession>
    <name>A0A1E5RAH0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g2486</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Mitochondrion inner membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the small Tim family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAH0"/>
    <dbReference type="FunCoup" id="A0A1E5RAH0">
        <property type="interactions" value="659"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAH0"/>
    <dbReference type="InParanoid" id="A0A1E5RAH0"/>
    <dbReference type="OrthoDB" id="274922at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.810:FF:000002">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11038">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11038:SF16">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 13" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="14">
        <location>
            <begin position="19"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ83892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="92" mass="10269" checksum="AB8AB6B6976CD168" modified="2017-01-18" version="1">MSFLGFGGGQPQLSSTQKIQAAEAELDLITDMFNKLVDNCHQKCINKQYNEGGLDKTESNCLDRCVAKYFETNVKVGENMQQFGQGFQPGRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAM1</accession>
    <name>A0A1E5RAM1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="19">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">AWRI3579_g2745</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 19">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Membrane</location>
            <topology evidence="19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="19"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAM1">
        <property type="interactions" value="373"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAM1"/>
    <dbReference type="InParanoid" id="A0A1E5RAM1"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140346">
        <property type="term" value="F:phosphatidylserine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000085">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000009">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF5">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 17 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 18 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 17 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 19" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="186"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="410"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="443"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="948"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="980"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1025"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1059"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1090"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1119"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="22">
        <location>
            <begin position="158"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="21">
        <location>
            <begin position="253"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="23">
        <location>
            <begin position="918"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="77"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="16">
        <location>
            <position position="502"/>
        </location>
    </feature>
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        <location>
            <position position="502"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <position position="502"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
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        <location>
            <position position="503"/>
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        <location>
            <position position="595"/>
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        <location>
            <position position="638"/>
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            <name>ATP</name>
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        <location>
            <position position="669"/>
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            <name>ATP</name>
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        <location>
            <position position="698"/>
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            <name>ATP</name>
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        <location>
            <position position="779"/>
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            <name>ATP</name>
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        <location>
            <position position="780"/>
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        <ligand>
            <name>ATP</name>
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    <feature type="binding site" evidence="17">
        <location>
            <position position="781"/>
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        <ligand>
            <name>ATP</name>
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        <location>
            <position position="865"/>
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    <feature type="unsure residue" description="D or N" evidence="24">
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            <position position="48"/>
        </location>
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            <dbReference type="ARBA" id="ARBA00001946"/>
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            <dbReference type="ARBA" id="ARBA00004337"/>
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            <dbReference type="ARBA" id="ARBA00049128"/>
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            <dbReference type="PIRSR" id="PIRSR606539-3"/>
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        <source>
            <dbReference type="RuleBase" id="RU362033"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
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    </evidence>
    <sequence length="1159" mass="130932" checksum="2C068F8DA292B208" modified="2017-01-18" version="1">MSFSQPGGRTFINHSRTNSSFQNSNDFTLEQDSLEQALDSIQFNNGIDISGSXSEATSKDDFELASLPEXEIQDHQTTFSDPLLSNKDSKQTAFDSPTKDNRETQHFKNLQTHKTNIIDWLFDIRENFGNRKKQAMSIKLDENNVEREIRVNTTPVFDRKKYPSNQLSNAKYNPVTFIPLILYEQFKFFFNLYFLLVALSQAIPQLRIGYLXSYIVPLAFVLLVTMSKEAMDDIQRRRRDAESNNELYEALNQSKLIPSKDLKVGDLVQLKKGQRIPTDMVLLQSTSDTPGECFIKTDQLDGETDWKLRLACPLTQKCTVDDLINKASITAEAPQKNIHSFNGKLTLNDVTSVPLTCDNTLWANTVLASSGSCIGCVIYTGIDTRQAMNTTKPKAKTGLLELEVNSISKILCLSVFILSVLLVLLSYFSSGDNADESYWYVDVMRYLILFSTIIPVSLRVNLDLGKSVYGYQIEHDATIANTIVRTSTIPEDLGRIEYLLSDKTGTLTQNDMELKKLHLGSIAYTQESFDMIKDQVAEFNTATGXKSLTKSKDPSRKSMSHKVYEMIVTLALCHNVTPTFEESGELSYQAASPDEIAIVKFTEEVGLSLFKRDKDCITLFHEASKTYLNFKILYVFPFNSNTKRMGIVVKQENTSPSGSEXEEYLFLEKGADSIMSKIVLANDWLEEETGNMAREGLRTLVIGRKKLNQKNFELFKKEYEKASISMINRDETMLNCIKTHLEHGLELLGLTGVEDKLQKNVKSSIELLRNAGVKIWMLTGDKVETAKCVAISAKLISRGQYVHTITGQKYETTSSQGLDKLEVIKSNASNSVLLIDGESLGMYLEHYKEEFFHAVIDLPAVVACRCTPQQKADVAHLIRQFTGKRVCCIGDGGNDVSMIQAADVGIGIVGKEGKQASLAADFSIEQFCYLTTLLLWHGRNSYKRSAKLAQFVIHRGLIISICQALYSICSGFEPLALYQGWLMVGYSTFYTMAPVFSLTTDYDIDESLSMLYPELYKELTEGKSLSYKSFFAWCALSLYQGVVIQGFSQHFTSLKDIDFTKMVAISFTVLIFNELIMVALEIRTWNKMMFLSEIVTFLLYVVSVPFLSEYFNLEYVLSLTFFVEMIFILSVSILPVWXIRALYRRLHLPSYAKVQQFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAT7</accession>
    <name>A0A1E5RAT7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NADPH-dependent alpha-keto amide reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2916</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAT7"/>
    <dbReference type="FunCoup" id="A0A1E5RAT7">
        <property type="interactions" value="214"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAT7"/>
    <dbReference type="InParanoid" id="A0A1E5RAT7"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF3">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="7">
        <location>
            <begin position="28"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="6">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ84018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="316" mass="36358" checksum="5457C2E87787A7C1" modified="2017-01-18" version="1">MSKINQKFFTLNNGNKIPAVGIIGTGSTWFKTVENDSTFSNELVEQLSQALKLPGIVHIDAAEIYKTYPEVGKAIKESTKPRNEIWITDKYSTALKISDTPVDGLNTALEKLNVEYVDLLLLHSPWIDQAAYGFGVVKAWKYLEQLYKEGKAKNIGVSNFGVEDLKLLLSSPEVEIKPQVNQIEYNPFLQEQTPGIYQYCQEQHILVEAYHPLGPLVLKNKAKENDEFVERNKEFYCLITELAKKYQKTESQILLEWTYEMNVLPVTTSSKFQRIKDAQYLFDPEFALSKEEVAKIRDLGSQNEPHRTYWKKEYTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAU7</accession>
    <name>A0A1E5RAU7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Guanine nucleotide-binding protein subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g3066</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G protein gamma family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAU7"/>
    <dbReference type="FunCoup" id="A0A1E5RAU7">
        <property type="interactions" value="96"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAU7"/>
    <dbReference type="InParanoid" id="A0A1E5RAU7"/>
    <dbReference type="OrthoDB" id="19232at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015898">
        <property type="entry name" value="G-protein_gamma-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036284">
        <property type="entry name" value="GGL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041848">
        <property type="entry name" value="Ste18_fungal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28189">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28189:SF1">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01224">
        <property type="entry name" value="G_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00224">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48670">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="G protein gamma" evidence="12">
        <location>
            <begin position="19"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="G protein gamma" evidence="11">
        <location>
            <begin position="23"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ84026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="107" mass="12435" checksum="97739FB75CF99069" modified="2017-01-18" version="1">MNELDVKQQQQEQLMYKIQYLKLRRVLELNNKLRQELSRERITASNACLKIIDYTTSTRDFAVPAMWGFPEQNTNPYNNNSAKKFNQYSPYGGSGPSSQEDTCCTIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAX8</accession>
    <name>A0A1E5RAX8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAX8"/>
    <dbReference type="FunCoup" id="A0A1E5RAX8">
        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RAX8"/>
    <dbReference type="OrthoDB" id="3972380at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="149"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="348" mass="39247" checksum="C95428EA935DD5C2" modified="2017-01-18" version="1">MSVSSRKSKKHVHNHRKLQITEPEIASSAAAIDNNVIESNTSSAQNQDNIDNSQDNSDNDAYDSTQENEPLLPESSAGTGNDDNANTNKRDNAKQNTMFVSPYVNVEIVDGTIIHTDNETGFSAITTAPSEDSGNTDSFNNANSTNHTSTFPAPKSSSGNSSHSKNQFVIWTVRISLQMQQQPTQLASPYHSLSKSALSSEQVEKQLYCYKRYSEFVVFRNQILKRLNRVLSKALAKEREYRNKIEADNDADQNGNTNTNSTADRENTAALRTQNRLVIKRLRDIHVPELPEPVSWWKCFSYNNINFSKNWLKHRQAGLEFFMCSVLLDNSIVEFCNDDIIKKFILNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB21</accession>
    <name>A0A1E5RB21_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">COP9 signalosome complex subunit 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3064</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB21">
        <property type="interactions" value="921"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB21"/>
    <dbReference type="InParanoid" id="A0A1E5RB21"/>
    <dbReference type="OrthoDB" id="10252687at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045114">
        <property type="entry name" value="Csn12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732:SF0">
        <property type="entry name" value="PCI DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732">
        <property type="entry name" value="UNCHARACTERIZED PROTEASOME COMPONENT REGION PCI-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ84096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="503" mass="58177" checksum="3CE79E0CF85E5E76" modified="2017-01-18" version="1">MNTAAQDIVVYSEELKYCIAQRRCSKLLESFISYGDSILANDPDLLYVTQQCDPAAFSPGKKELLLSYKEAQQRHFFNASPWVFHSCDNISSKLNENITNFVSVTLNLKKKVKDFGNGILPVESTIPELFDLSDQQLKCFNRIADGQTFWITLPLFSIAKQFFKFSYGLDQAFSKMSINLSDAAPEQQSVGDLQRELYLERASRTIQTSYKICLNDREPNILFSKLCNVYCFINLNFAIYEKLQKSDLEKAYVKVLQDRLLKNQLPDISNKNYSNFDKLWFNYFMGKHFISLNDLSKALPYLIESFNYCNIYCTTMFENIMNYLIPAYISHHQAIPHFILGNKVIPESWADFVEFLKKGDLAGINLWIHTNELFLLQQNLMPIVTHIKDLCTARFLQKIWVINGKHWIIDSKEFALLWKNTLNKETEKEDAEILNFLAGKDKSNTDLETSXVNLDDLMNQAENIFANLIAKRFLNGYISHEKRKLVVSKTNPFPVFQTRPPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB32</accession>
    <name>A0A1E5RB32_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">37S ribosomal protein MRP21, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3031</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RB32"/>
    <dbReference type="FunCoup" id="A0A1E5RB32">
        <property type="interactions" value="119"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB32"/>
    <dbReference type="InParanoid" id="A0A1E5RB32"/>
    <dbReference type="OrthoDB" id="2501249at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070124">
        <property type="term" value="P:mitochondrial translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052837">
        <property type="entry name" value="Mitoribosomal_bS21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001911">
        <property type="entry name" value="Ribosomal_bS21"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00030">
        <property type="entry name" value="S21p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41237">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN MRP21, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41237:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS21M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01165">
        <property type="entry name" value="Ribosomal_S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ84107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="22920" checksum="E246C11EBCB9D769" modified="2017-01-18" version="1">MFALRNQKYLGLVSTFKNQSLLLSTRLFSCSQISMQQGGQGKTGADDVLSNLVKDLNKSTSSSPSASAKFDNILDPSKLQKKKRFTFGSIDNNNPTMFGARGKESFLPTTQTIKRTVDEYHLTGPRAGRTVQVRNGDINMATRQLNRMLNDAKIAQTTMDQRYYIRPSKQKLLKKVRRKKREFSLAFGDLLRTVQEIKRKGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB92</accession>
    <name>A0A1E5RB92_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">ER membrane protein complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2792</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The EMC seems to be required for efficient folding of proteins in the endoplasmic reticulum (ER).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMC3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB92">
        <property type="interactions" value="429"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB92"/>
    <dbReference type="InParanoid" id="A0A1E5RB92"/>
    <dbReference type="OrthoDB" id="6745403at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008568">
        <property type="entry name" value="EMC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002809">
        <property type="entry name" value="EMC3/TMCO1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13116">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13116:SF5">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01956">
        <property type="entry name" value="EMC3_TMCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010045">
        <property type="entry name" value="DUF850_TM_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01415">
        <property type="entry name" value="DUF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR010045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ84165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="276" mass="30860" checksum="BA0EF93FB3FD78CC" modified="2017-01-18" version="1">MPSGYASLAAVAGTAIELTLDPQLKYNVLLPITLVIVLTGILKNYIMLLLGPKIPTQPRVNITEQQYLQKGQTLLINGKYLSYEGFKMRQQYMAEALGECKYSAANAVEKSADSAEEEQGPXNPLTDPNASDAMFAMAKGNLMNFIPQTLIMWWVNHYFAGFVLMKLPFPLTVKFKEMLQAGVMTSDLDASWASSISWYFISMFGLDPLFNVLVPSAGMDVMQMQQQQQQQQNPMANMNPNAVSDAMKSLSNDVFIGTYEQEEYANLENRILKMYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB96</accession>
    <name>A0A1E5RB96_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein BIR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB96">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB96"/>
    <dbReference type="InParanoid" id="A0A1E5RB96"/>
    <dbReference type="OrthoDB" id="4070233at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001370">
        <property type="entry name" value="BIR_rpt"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50143">
        <property type="entry name" value="BIR_REPEAT_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="348"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="402"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="482"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="527"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="759"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="486"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="531"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="763"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="777"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="908" mass="102158" checksum="FE89A45224AFECD5" modified="2017-01-18" version="1">MEQRQRAQTFEDSIPLLDNSKKQCSWPFGSMSGELLAECGFVYAPRTAAKANIKCVFCNVQHGAATNESESKSLRQTVKHILKKHLKESPTCLLSLLKYCTLSNSFLQFYQKDISMQDYQXLQKWTFSDSQISKDAVRELIKVGFLYYLPIDSPTFIESTTGKKEHYGRNHTLKDNIEDAHXFCPYCNKAFNYTKQKKKITENPYDFHLRASPKCYFLLNLFTMRKDSSKTMLEVXPQKQLEXIAIXDVVDDAVVDDNKEENXNASQKDSSHPSNAKQIENDSFRDKTTLKEVTSLNLNLAQPIXEKPAASKKRKLLLGSLTTKIGESLAESSRSDDEFSRESRISIANLDTGSQRATRFNGKNLQSTTSQNQPDTAKSNQVNRSLKSSPIKIDFFRNINSPKKNHLLDSDNSSDTEGEDIKQIERPGKVIQENALISTTESATASPNRTLKRKLMAIPIPHDDLHSLSDSSEEESYNXIASTNEISREVSLPKEXPTDEKVEQANVSTSFTRQPLVLNSSYKMLKGVRLSPNTRPSTHSLSNKQEQSDIESITSSNASLNSEFQNNGKTNTSSQGREVEHDQLDKIXMPLQIERLQQGPIDTKNMDGKLLQKAEASASNIEKLDNIESQFLVNENNVLVKSLSTEHLDLNNVTNSANHSALKSQLEIKSGKTKSPLVNLKNSKIFXEKTTSNLSNAVIEPGVVLEEDISPVKMTTNINIDSSPLKSTKQPTRNINATLKNMQKFRLQTTLGTSALITDEKPMTQNPSSVARTSELVSEKQKKLSTETENKKEGLQLSNDDSDLILASYLRDLLRYINQNDATLANDRLGELHYFFQSMPNEERNMTFEDWLECQCEKLKQDLLHLHEQKLGILANKFKSLIELVKTSEENVLSELAKELGIANPVIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBN8</accession>
    <name>A0A1E5RBN8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2751</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBN8"/>
    <dbReference type="STRING" id="56408.A0A1E5RBN8"/>
    <dbReference type="InParanoid" id="A0A1E5RBN8"/>
    <dbReference type="OrthoDB" id="1925334at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050668">
        <property type="entry name" value="Cytochrome_b5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="7">
        <location>
            <begin position="20"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="121"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="126"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ83993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="227" mass="25765" checksum="5B3430C5A637562F" modified="2017-01-18" version="1">MAADQEFTTHEETRQMQRGLKLVSREEIEKHIELGECWITIHGNVYNVTHYKHPAGSAILWKYNGKDCTKQFDEIGHSMESLIYDIDSSCLMGPSEEAHLEALEREKLSEFRSIQKQGSIIGGAGRSRQGSARQRSSVMSSQSRTSTSSSNGSTTRKRFGSISKKKCFCRIFNDYDRQKYHLALSLLSSILFFGGLLFYLKMKRLKHTTSIPLGSLSDPPVLFMQEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBQ5</accession>
    <name>A0A1E5RBQ5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Vacuolar transporter chaperone 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2865</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBQ5"/>
    <dbReference type="FunCoup" id="A0A1E5RBQ5">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBQ5"/>
    <dbReference type="InParanoid" id="A0A1E5RBQ5"/>
    <dbReference type="OrthoDB" id="2243669at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033254">
        <property type="term" value="C:vacuolar transporter chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051572">
        <property type="entry name" value="VTC_Complex_Subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140:SF1">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE COMPLEX SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="DUF202" evidence="6">
        <location>
            <begin position="24"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ84316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="123" mass="13613" checksum="FBE55470A56FBE2D" modified="2017-01-18" version="1">MSSAPLLQKAPGKKIALPTRVEPKVFFANERTFLSWLNFTVMLGGLGVGLLNFGDKVGKISATLFTLVAMGTMIYALVTYHWRASAIRRRGSGPYDDRLGPTVLCFFLLLAVIVNFVLKLNYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBQ8</accession>
    <name>A0A1E5RBQ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">eIF-2-alpha kinase activator GCN1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2816</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GCN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBQ8">
        <property type="interactions" value="1487"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBQ8"/>
    <dbReference type="InParanoid" id="A0A1E5RBQ8"/>
    <dbReference type="OrthoDB" id="5148094at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904688">
        <property type="term" value="P:regulation of cytoplasmic translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000090">
        <property type="entry name" value="eIF-2-alpha kinase activator GCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022716">
        <property type="entry name" value="Gcn1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056810">
        <property type="entry name" value="GNC1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057546">
        <property type="entry name" value="HEAT_GCN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056809">
        <property type="entry name" value="HEAT_GCN1_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346:SF7">
        <property type="entry name" value="STALLED RIBOSOME SENSOR GCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR GCN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12074">
        <property type="entry name" value="Gcn1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24993">
        <property type="entry name" value="GNC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24984">
        <property type="entry name" value="HEAT_EF3_GNC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24987">
        <property type="entry name" value="HEAT_EF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23271">
        <property type="entry name" value="HEAT_GCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25801">
        <property type="entry name" value="HEAT_GCN1_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24916">
        <property type="entry name" value="HEAT_GCN1_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TOG" evidence="5">
        <location>
            <begin position="1311"/>
            <end position="1542"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="4">
        <location>
            <begin position="1483"/>
            <end position="1521"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="4">
        <location>
            <begin position="1602"/>
            <end position="1640"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="4">
        <location>
            <begin position="1954"/>
            <end position="1991"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00072275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ84013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="2678" mass="298150" checksum="866630CFC414324D" modified="2017-01-18" version="1">MSEILDPDFLGQPVKTRIAHLATLHDSIEDSPSCLSENESKFYNKLSLKLLESHGTFEDRESKELVTAIFKKIIENSEFKMLPKYTTFIDLFISNSNPSAHATMDYIHILEWILSFFDLVLSSPNHESFDENLIKLCDLYVATTESIELSYSGSSKKATHDQRLRIYVQKISVRCFSSSKINLIEKLEAASYFDKVFTKGPISGSVTLLGALTSTYLHKSNNNPGQDFANSPFVKKSCEFIGKQALLGKTIPTPEGFVTNLKEFFIAFMSKDLFVEFIVPNLEKMNLRSAESAFHLASRLYASDLLQFQFGEIITSSKLMPQTIGGLKNSKESTRAYAFQSLIALMKNPKFDHSSHEKIVDELFKNLKSNLNSEYKTLVAQITYEIPSESDAASHKVVTGLSSYIQKESNETVLATMLQAFFKHCNTLNAEEFKSFSTVIDKGITEKKLPIKKLWISSFLLSLSKSTSSAVFEYLLHASQLLPFVEDLLANTHKNDHSLILGAVKLLERVEFYDVFKSEEQKQNLGLNLVQVCLSQSLSAKQRLESISVLETLFCKNPELISTSTISGLEFVFMQDAQAAEEEYHILEKYVAPLXTSFSTPIXNKDLSATIMIDLLYCTQKASKYLQKSMGWAGLVLKAQMDPKVLLAEKLEYLVSRFEKYSENLKVTESSLGDFVFSSMAYCSFINSEVLAPVIANKIYKNIESNLENLQNLSHEDIYIGKHGVDGVMTIDVIEALAMKKASQVSKNSKEYETMKWEESIRKKKAAAGTKTVSRKYTKEEQILVNEQLEKEQTIRAKVAQTFNSLNMTIKLVSALSFDAKQVDNGLSYWYPVAVDELLKLLKIEQYHALLGSLPLETFLSLSNALTDRLGSSKYFVGVAILRSYNVAQIPSNLEEEPLLELISRVLFRIKFLTDKIPFSSSNLTFILPLLTKVLEEGKKTAIKNSTKQTLKTEFVEEDKEEEHLMLAIEIILSHAEEFQNSNIPRQNILGVLLSLLSISSKAKMAKDCFNTLCQNISVSPTESDLKMILSSIMTPNQFVRTTVLETLDEEFDLQPHMKCPAEIFISLFDEEESNREYAQMIWETNSFKLEESVMSNLLQNFFQQEDSGLRLFIARAYANAAKLLGDMENGKSFYFYVQKLMDFYIELAKPLQDILDEYGLIAVYASQQKDPWESRSTAAIALAECVQYYXKETPAEEIANFVKFLVDTGALGDREPLVRQEIKEAGIAMLTYHGSSNVENLIPIFEHSLATEGDVTVKENVVVLYGSLAQHLSADDSRVGDIVCRLLDALTTPSEDVHQAVAQCISPLVHLFRARVSEFVSNLFVQLFDSAAPAHVRKGAAWGIAGLVKGYGISALSELDIIRNLIEASEDKKDAKKREAVAYSFNTLSKMLGKYFEPYVIQVLPYVLKNLGDASLEVRDATTEAAKTIMSNSTSFGVKKMIPVAIDNLEDIAWRTKRGSVELLGSMAYLDPTQLSASLSTIVPEIVGVLNDSHKEVRKAADQSLNRFGEVIRNPEIQKLVPVLIKAIGDPTKYTEEALNALIKTQFVHYIDGPSLALIIHVIHRGMRDRSANTKRIACKIVGNMAILVDAKDLIPYLDTLIAEVETAMVDPVPNTRATAARALGALVERLGEDQFPNLISKLIDTLGDETRAGDRLGAAQALSEVMSGLGLYKLDELLPTILAGVTSYRSFVREGFIPLLLFLPVCFGAQFAPYINQIIQPILAGLADNNDVIRDTSMKAGKLIVKNYANKAIDLLLPELEQGMFDENEKIRLSSVQLTADLLFQVTGISSKNEFAEKEQEEDEDDISAQHGEVSKQMVEVLGLERRDRILSSFFVCRSDTSGLVRSATVDVWKALVPNTPRTVKEILPTLTNIIVVHLASSSSTLRSIAAQTLGDLVRRVGANALSQLLPTLDESLESSTDSNSRQGVCIALHELIESSNVAVLIEFQEIIVSIIRKTLVDGDETVREAAAFAFDSFQTVIGKTAVDEVIPYLLNMLNSDDNSEYALLALQEIMATKSEIIFPILIPTLLAPPIDAFKARALGSLAEVAGSALFKRLSVIISALVDSLLITNTDSEDRNEIASALDKVFVSVKDEDGLHPLLQQILALTKHEDSLKRAVILERLPYFFENTSLNYNIYTQDFVTQLILSLDEKDEEIVKNAFVSLSALVKKQNKNTLESLVKPAKQALELTGKRGEDLFAFSLPKGPNCVLPIFLQGLMYGTSDERECSALAIADIVSKTPAANLRAYVTVITGPLIRVVGERFSSDIKAAILYALNVLFGKVPQFLRPFVPQLQRTFVKSLGDSTNETLRLRAAKALGTLIEYQPKVDPLVVELVTGAKQSTDFGVKTAMLKALMEVVSKTGNKLNETSKINIVQLVEDEILNVNNEKLAIAYARLIGALSEILKPEEAHKILTDKVLHAGSSGEVQNDVEXLKFGVLTLNAFLKDAPQHIFSMKEEFVPYMLELSENTLPYISDNILVAIGKFLLLENEVKSPYSKSTDPDSPFEVGTDNMQKLITKLCQCMLAPASNSLDSRRLSLIIIRTLARFKYDDCVKPYLDIIAPNCFSCVRDTIIPIKLAAEKAYLAIFNLVSDVDQNVFQAWLKAQEGKDISNSVGTSLMQRSIAEYTKRVATRLAKVERERIEAGGDEEAMFSDRFEDENEIWAIGGVDLNTDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBU4</accession>
    <name>A0A1E5RBU4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 19">Autophagy-related protein 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">AWRI3579_g2874</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Phospholipid scramblase involved in autophagy. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate)(out)</text>
            <dbReference type="Rhea" id="RHEA:67920"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out)</text>
            <dbReference type="Rhea" id="RHEA:38663"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phosphocholine(out)</text>
            <dbReference type="Rhea" id="RHEA:38571"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out)</text>
            <dbReference type="Rhea" id="RHEA:38895"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 19">Preautophagosomal structure membrane</location>
            <topology evidence="3 19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 19">Belongs to the ATG9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBU4"/>
    <dbReference type="FunCoup" id="A0A1E5RBU4">
        <property type="interactions" value="251"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBU4"/>
    <dbReference type="InParanoid" id="A0A1E5RBU4"/>
    <dbReference type="OrthoDB" id="3972909at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007241">
        <property type="entry name" value="Autophagy-rel_prot_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13038">
        <property type="entry name" value="APG9 AUTOPHAGY 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13038:SF10">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04109">
        <property type="entry name" value="ATG9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 19" id="KW-0072">Autophagy</keyword>
    <keyword evidence="14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 19" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 19" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="375"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="545"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="630"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="73"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="920"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="985"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="16"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="122"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="188"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="924"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="954"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="985"/>
            <end position="1018"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00024621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00024631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU364027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OEJ84356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1055" mass="122023" checksum="FF0BDF5FD29D5F2B" modified="2017-01-18" version="1">MDSSSIMFSKNDESRSSSQKEQVQSTNRNTFLSGIFNKTKPTDDESDSARINIQHLNNANFESADSIALNSLSESNSSMQSTLDDNLITQHFATDRMIESDQETSSSSESGEEQDEQAKDETDQEVELEVEVEDDDNDEHDEQSDNDSMKYFRANIHTLDTNDDGTTDKKQKQGQEGQDTDQEKKKSMNTNYSISNGLSMDGSPFGLPKPNNNINKKKLFQNNLPKQETNDIQKSFLFRKKSNFPTPGNMNDNYSNIDLESNQNGQKDYVQPAYTATMRQNIISLSKKEKALWKWANIENLDLFLIKVYNYYLGSGFKCILVEKFLNLFTLIFITFFGTFSTNCIDYSKFHQAQKLQDIVVEQCYKNSMTNGTKFLIYLCMLYVVLKLITIYNDYKELLEIHDFFSYLLEIDDLELQTISWQNIVSKIMDLKDSNALTANVHEIKAKNRINAHDIANRIMRKENYLIALFNAKILDLDLNVPLSLRFLPPTARSINLSSNLTKTLEWNINLCIIGYAFNEQGYLKHYFLKKSQRTKLIAELKKRFMVAGFLNIILSPILVSYFVLLYFFRYFHEYKSTPGAISTRQYTLRAEWKLREYNELYHLFQKRLGLSIEIADDYVNQFPNEFKNLILKFIAFITGSFVAILAIFTLFFDSENFLNFEISKDKSVLFYLSIFGTVYTVCKSSISDQYKVFDPELYMLELVKYTHYQPKKWENKLHTQYVRDDFCRLYDLKLVVLLKELSSLLVTPFILWFKLPSKSDEIVDFFRNVSIYVDGLGYVCKYAVFDKENDTLNSDGISDGKSRKFTKGQKKQSIRTILKKRYESNPGMQDNTEINNKPAKQKIKYEIEQDDKHDNEEINDKIMQSYIHFVDNYSNVENNVGKTAIPRHVELNGNYTWKKQFGFSNNMKRSQLLTAENGLRPTESLSPNSSSHNLGQQSRLGHKSSNEDRGLANLNQGSKFGETQQGFGKQRRPNLFESDSLINQSSSYQDQPSSIITDPNFGPNTTGYNNHTNQNHNMADHSIYQGRGNVDQETNTKRVLGLLKQYYKDADVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDL3</accession>
    <name>A0A1E5RDL3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">GPI ethanolamine phosphate transferase 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g2304</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGG/PIGN/PIGO family. PIGO subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDL3"/>
    <dbReference type="FunCoup" id="A0A1E5RDL3">
        <property type="interactions" value="660"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDL3"/>
    <dbReference type="InParanoid" id="A0A1E5RDL3"/>
    <dbReference type="OrthoDB" id="272139at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051377">
        <property type="term" value="F:mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16023">
        <property type="entry name" value="GPI_EPT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037675">
        <property type="entry name" value="PIG-O_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039524">
        <property type="entry name" value="PIGO/GPI13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23071:SF1">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23071">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="461"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="497"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="528"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="569"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="596"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="732"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="765"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="804"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="844"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="948"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="981"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1028"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="18"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ84987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1060" mass="120849" checksum="180A26C9BB1850F2" modified="2017-01-18" version="1">MSQHQDSPELFDEATVRKSMNLSRRSNTTKKPVNSSDTTKNNATDFEKSDELRVYRQNTFRFRIAHTKYIFLMFFLGLLQLFAVIFFCKGFLLTRNVIGDVATFDVQLQGLSEKSFANEKNQIVFNKTVILIIDALRFDFVSPQPHSNEVYHNHFPILQNAPHSYLYKFLSDPPTTTLQRLKGLTTGSLPTFIDAGSNFNGEVIDEDNLIKQFHLNNKTTYFAGDDTWKALFDPYLSKKSKYYESLNVWDLETVDQGVMRYFDEHLTEEDEEWDVLIGHMLGVDHVGHKHGPNHHEMSTKLEQMNAFVSEQLLPKIDNDTILFIFGDHGMDHTGNHGGDSKDELETTLFVYSPKFGVTPPKSTNHDHKVVNQIDFVPTLSLLLNIPIPFNNLGWPIEDIIENSGFAQTLNQIHHYQETSKVLEGNPLLLTNLNTHFNKNEGAIYQRLFLENCKSMWAKFDLISISIGITLLSISLCLIIVITKLIPSIVVGQMVHEFVPSIIVMCMISCVSLNSIFQILNQPSFLENWKFCTLFSLAIGIIVGCCIPIFDRYTIKWMAIKFFEEINDYWSRVAVLMITLHALCFTSNSFTIWEDRIVAFLLTTIGFLTLYEFAFAPKRASTNALFTAASSRVSNMLSKTQHSTFVSSWTHRSHSPDKQHSSDLHAANEGENVSDNTASSETLPLGRFARVIGAYHSLLLILATRLASNITICREEQGTKCIPTFVNANNYSWWAMLGIFLLIWSLPTCIKGYYNISSSYQAAAPVWINQFLKSSLFLSFIYWFLNYYETTYGSLPFDAQIWKFSISRIVAGFSLVALNIGWAMGPMCIKLDLRNTDLKSQTATILGYSNVYGAQFFLLVINFVMAIMFFSKPLAQLSLFLMINQLLSILEIFDLLKLKENLIGPVTLGLLAYQHFFSTGHQATIPSIQWDTGFMLTEKITFPFTHISLILNTFGPFFIVALSVALLTLWKQPPSVLKPQTLLSRIVSNCGMLLIYNTVLCLSSFAWVTHFRRHLMVWKIFCPRFMFSCMTLLVIQVVVIFVTVAFASGRLIRQINNIFWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDM9</accession>
    <name>A0A1E5RDM9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2305</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RDM9"/>
    <dbReference type="OrthoDB" id="4097087at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ84986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="371" mass="42591" checksum="D4D1523748BE2040" modified="2017-01-18" version="1">MSENFEQNALLGNSDNESNKKEQFDLKVMNLQGRPVTLKMHVHLVAFPTLNDLILHFQSLGNTFTASTTETRAKYTHELLRMLEGNQSLSGNEVFXKDKSNASSPNGQELDSIKSLVXKKKVFFKMYSKEKVDEFNAKHRAMGKVIPIETVLTLKDLIVLEKTFHKYDPNSCHLVSKDTEGKELLGXNASPPFVPSKIHEANDVLRDVRSAESNCSPSHVHSSLKTENQRTQILPEPKEQMPKHHFSKHITVTSVFKNEPIFDKIINHLPRTKTEFAHDSKLAFVKVYRKSKTNLTKLELVPIKKLNLQNDNMESIKKHICYPRYKHNTKLFIVGDVDYFATIFMDYNQFLEKIIWQNDSTLPYDLVGVIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDP8</accession>
    <name>A0A1E5RDP8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDP8"/>
    <dbReference type="InParanoid" id="A0A1E5RDP8"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ85027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="80" mass="9649" checksum="A05393BB507AE73C" modified="2017-01-18" version="1">MSNSNVQFRVFNLQQPRYLLVNNYKNNNKNDDDDDYENNRLTSQLINNFKSQMDTTRFFDADGYDPSIIESKFKFKFNNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDZ0</accession>
    <name>A0A1E5RDZ0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDZ0"/>
    <dbReference type="FunCoup" id="A0A1E5RDZ0">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RDZ0"/>
    <dbReference type="OrthoDB" id="3978317at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="98" mass="10595" checksum="2F550D973B017CF0" modified="2017-01-18" version="1">MSTILNNTDNNTTMQPPSFYKNTNDSTQGSSSSFFQSFPVDLTTVKGYLEFIQKKGGPSKVITEFNEDKSGGVVLNGKGDKIATVSGEAMEYLIKLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDZ2</accession>
    <name>A0A1E5RDZ2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acyltransferase LovD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1943</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDZ2"/>
    <dbReference type="InParanoid" id="A0A1E5RDZ2"/>
    <dbReference type="OrthoDB" id="428260at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050789">
        <property type="entry name" value="Diverse_Enzym_Activities"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43283:SF3">
        <property type="entry name" value="BETA-LACTAMASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G07500)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43283">
        <property type="entry name" value="BETA-LACTAMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Beta-lactamase-related" evidence="1">
        <location>
            <begin position="53"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="436" mass="49849" checksum="ADF4D10519E80544" modified="2017-01-18" version="1">MPIKIELSQQEKNIKIQEILDKYTSRNTLESPIVPGVVAGYATEKEIKYVGASGVLKHTESKXRGENVADSQTDLANKMETDSLFLLWSTTKAVTVTALFQLIERGLVGLHDPVEKYLPEFEFKKVIKSMDYETAEMELEELHIKPTIHHLTTHTAGFGYFFFNKYYKTIXDMGNSGNXLNCTYREFDNVPLSFQPGTRWEYGTNIDFVGAIVMKVSGLSLEEYFQKNIFDKCDIHTMKFNLVDEKDHQTLSNIHIRDTTDPTKFIQVTEKLTPSTPDFECGGHGMFGTVGDYLKFLQIFINDGVSPITKERILSHETLVNHAFKDQLKEMALQPGNENCYFKDLPSTDAAVTNPVSVYPGVKKGWTNFFMINEEYIEQVNRPKNSLMWCGLANLYYVINLEKKLVMYWAESVFPFADISSFTGFSEFEKFVNTEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE13</accession>
    <name>A0A1E5RE13_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dolichyldiphosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2379</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RE13"/>
    <dbReference type="FunCoup" id="A0A1E5RE13">
        <property type="interactions" value="358"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE13"/>
    <dbReference type="InParanoid" id="A0A1E5RE13"/>
    <dbReference type="OrthoDB" id="302705at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF59">
        <property type="entry name" value="DOLICHYLDIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="2">
        <location>
            <begin position="2"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="175" mass="20147" checksum="57CE3A6249C9F9A9" modified="2017-01-18" version="1">MAGGHVVNDIVNNIVKKFIKEPRPFNYFGDSFQQNTIRSGYGMPSAHSQFMGFFAMYVGLRLFTFWDTSLFNKIVGTIALIAVSILVAWSRVYLGYHSFTQVGVGLSLGSALASFYFLVVIYVRRVGLSQWFLNWKICQKLYMKDSAFYRPLSLKQEYDAWKTRTSNINEEKKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE70</accession>
    <name>A0A1E5RE70_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">methylated diphthine methylhydrolase</fullName>
            <ecNumber evidence="6">3.1.1.97</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2050</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>diphthine methyl ester-[translation elongation factor 2] + H2O = diphthine-[translation elongation factor 2] + methanol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42656"/>
            <dbReference type="Rhea" id="RHEA-COMP:10172"/>
            <dbReference type="Rhea" id="RHEA-COMP:10173"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:79005"/>
            <dbReference type="ChEBI" id="CHEBI:82696"/>
            <dbReference type="EC" id="3.1.1.97"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DPH7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.97" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE70">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE70"/>
    <dbReference type="InParanoid" id="A0A1E5RE70"/>
    <dbReference type="OrthoDB" id="1930760at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061685">
        <property type="term" value="F:diphthine methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052415">
        <property type="entry name" value="Diphthine_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46042">
        <property type="entry name" value="DIPHTHINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46042:SF1">
        <property type="entry name" value="DIPHTHINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ85202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="374" mass="42188" checksum="78C4519EC98B3FDA" modified="2017-01-18" version="1">MSDLEVETATLSAIKTLYPPCCLQILPDNEHILVGTYELQKESGKRIGSIDLYRFDASTNKLSLVYRFSDNVSAILDLKLNGMNKFVTAHSTGELRLWEYIFDGEKTFELKLVQKYQVFQSDVLVTSCHIRNGYGSSENNIPEGNEVLCTGTNGELKLVDLSQYTENNVSESVYEFDSAHSLECWTAEFGKLAPLENCVLSGGDDSVVKLFDTRTRNEVWSNSRIHEAGVVAIKCASPSFRNXRPTSILTGSYDDNIRSFDLRMLSATTIYPGTMPPTNXKSKNLGGGVWRFVENPFYAQDPTVDEILVCCMYNGAKVXKIFDEDEXEQNEESIFKEQNYVKKGHDSMCYGGDWSKSFIATCSFYDNKLQVWRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE82</accession>
    <name>A0A1E5RE82_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RNA 3'-terminal phosphate cyclase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1831</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNA 3'-terminal cyclase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RE82"/>
    <dbReference type="FunCoup" id="A0A1E5RE82">
        <property type="interactions" value="822"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE82"/>
    <dbReference type="InParanoid" id="A0A1E5RE82"/>
    <dbReference type="OrthoDB" id="1911237at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00875">
        <property type="entry name" value="RNA_Cyclase_Class_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.20:FF:000004">
        <property type="entry name" value="18S rRNA biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013791">
        <property type="entry name" value="RNA3'-term_phos_cycl_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023797">
        <property type="entry name" value="RNA3'_phos_cyclase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037136">
        <property type="entry name" value="RNA3'_phos_cyclase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000228">
        <property type="entry name" value="RNA3'_term_phos_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016443">
        <property type="entry name" value="RNA3'_term_phos_cyc_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020719">
        <property type="entry name" value="RNA3'_term_phos_cycl-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036553">
        <property type="entry name" value="RPTC_insert"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03400">
        <property type="entry name" value="18S_RNA_Rcl1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096">
        <property type="entry name" value="RNA 3' TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096:SF1">
        <property type="entry name" value="RNA 3'-TERMINAL PHOSPHATE CYCLASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01137">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05189">
        <property type="entry name" value="RTC_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01287">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase" evidence="5">
        <location>
            <begin position="18"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase insert" evidence="6">
        <location>
            <begin position="192"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="376" mass="41236" checksum="70A32E3325F7B38C" modified="2017-01-18" version="1">MSVNKKANGTSSASRYVTFQGCKNFRLRIIMAILSGKAVKIEKIRSDDMNPGLKDYEVSFLRLIEAVTNGSSIEISYTGTAIIFKPGIVTGGSYTHACPNSKPVGYFVEPMLYLAPFSKKKFSLLFRGITASHTDAGLEAIKWGLLPVMEKFGVRECALHTLKRGSPPLGGGEVHLVVDSLISQPITMHALDTPMINKIRGVAYSTRVSPAMVNRMIDASRKVLRDKQVGCEVNITADVWRGENSGKSPGWGITLVAENKQGWRYFSQSISSPGDVPEDIGTKVAYQLLDEINNSSVVGRNQLPLALIFMVIGKEDLGRLRISKNQIDSKFVQLLRDVKKVFGTQVILQECEDGDESDMVLTVKGIGFTNTNKKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REF4</accession>
    <name>A0A1E5REF4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA polymerase II subunit A C-terminal domain phosphatase</fullName>
            <ecNumber evidence="6">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1668</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">This promotes the activity of RNA polymerase II.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REF4">
        <property type="interactions" value="833"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REF4"/>
    <dbReference type="InParanoid" id="A0A1E5REF4"/>
    <dbReference type="OrthoDB" id="10249888at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008420">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd17729">
        <property type="entry name" value="BRCT_CTDP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039189">
        <property type="entry name" value="Fcp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011947">
        <property type="entry name" value="FCP1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02250">
        <property type="entry name" value="FCP1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23081">
        <property type="entry name" value="RNA POLYMERASE II CTD PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23081:SF36">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT A C-TERMINAL DOMAIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="9">
        <location>
            <begin position="227"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="8">
        <location>
            <begin position="525"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="132"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="682"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="138"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="682"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="720"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="751"/>
            <end position="763"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="769" mass="88678" checksum="1D2E09F21B51C337" modified="2017-01-18" version="1">MFEEDATAEKDLLTPIRLSSILPFPITIASIEVSPNDKVKKGDKLLMFKFWIFQDVXVSADGDQIETKKERIDQVGSFESPYSGIIQNINLKVGDELINNTDVIMKIKQECLHPMVYGGLCVVCGQSVEEENEEDSMQSKQNQQQNRSSSQDSQHDFSNQLYNKENQRNGQQQLHSKXQQQFFXNNQSSYNVLDDNHQIVMSHTNTHIKVSAREARNIANNHMKSLLKQKKLILVVDLDQTVIHCGVDPTIGEWIKDPQNPNYDLLQEVKSFALEEDISSWLKSQPFFVQQQFHAPLHKTKNWYYVKTRPGLKEFFEKISEYFELHIYTMATRAYALEIAKIIDPDGSLFGDXILSRDENGSLTHKSLERLFPTDQNMVLVIDDRGDVWKWCDNLIKVVPYNFFVGIGDINSTFLPKQDTTLLPEGLHNKEVQNVSNEVHEKELSKVDEIQTDDKHSVQIETQLESRPLKEVSQHFANGVKILTDNDIELKKLGPKLVDIHHSFYEKYFENTKERVDVRDLINSMRNSVFASKEFVFSGIIPLNTDFNKVDIVQWTRSFGGNVSFDLTRTTTHLITRTPGTFKARLAQKFNSEIKVVHPDWLFECFLNWKLVSCEKYVLQVHGPAISDEEYEXFLETLELRETKELERKQAMEREQSGVVELQYLGGDESWLDNNEDELDDLLDSEEENQESESGSENEESADLQRETQEPSDQVNGKKRPLEEEVEFKGGFEATLSYKKRKSEEEKDDHGDSEDESDFEAELLQELGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REM0</accession>
    <name>A0A1E5REM0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Signal recognition particle subunit SRP72</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1892</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the SRP72 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REM0">
        <property type="interactions" value="961"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REM0"/>
    <dbReference type="InParanoid" id="A0A1E5REM0"/>
    <dbReference type="OrthoDB" id="5421607at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013699">
        <property type="entry name" value="Signal_recog_part_SRP72_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026270">
        <property type="entry name" value="SRP72"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14094">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14094:SF9">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08492">
        <property type="entry name" value="SRP72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038922">
        <property type="entry name" value="SRP72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 9" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="Signal recognition particle SRP72 subunit RNA-binding" evidence="11">
        <location>
            <begin position="501"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="510"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="512"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="537"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="594"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="611"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR038922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ85003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="622" mass="70763" checksum="2FE994BB86446C4C" modified="2017-01-18" version="1">MSDLTQLLKKLDIDDSSSTLQQVEHVSLQNLPQSLKPYLISLIKQDKYEVALRALQKYASEKTIXNLVLEKAYIYYKLNKKSDFEQLLKKIDASELSXFELRGLDHIKAQFFYKYGDLEKAFTIYKSLIAGAQSGMDDLSELEVNKECTSQFEGAANFTEQEGIXYDLALNRSIAAATAGNYFSARQFAEQAASLAQEKEELLMAQFQLAYLNKNVKEKKDLLKKIAXNRDNLLLQSLAKNNLQAIECSSFSTVLTNFPLVLSKMDLPAINVLKSSASFSTRQNSLISSNILLLRLFSNSSINENKSSLPIKYALEKYSELVQDMTMEPYSTQSKKLYHYVLKDVKNYKYDEEKHIALLLLAVQLQIQTKTYENAIRLVEEYIKTHEAFLKVHTFALLSIILLKLYKSMNRRSDRRNLLLKIQSYKPLEETLLPKANGYTEFWEYVAYEFLELGLSKKYTEVLTRASIPLPENSESDAPKFSFKCDVNKLIKLGVEPFVAKVKVVQNNKPGKILKKKPKKKSKKAPKRLPKSYDPARVPDPERWLPMKDRTSVMLKKSARKQRKLLAEGKTTQGGGLNNKKLARKLDISKKKTASATTASVKSSKSNKSGKSSKSKKKSGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REM7</accession>
    <name>A0A1E5REM7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ethionine resistance-conferring protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1910</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REM7"/>
    <dbReference type="InParanoid" id="A0A1E5REM7"/>
    <dbReference type="OrthoDB" id="2126698at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="530"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="557"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="7">
        <location>
            <position position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="598" mass="66427" checksum="26EE51F59EE6B804" modified="2017-01-18" version="1">MSLGTHSFETSSRRSSIYVRPPVIAPSNNVLLSTVPSNISAACTESDIQVSPLANPAFTTTVLHETKFLFTNSAPLVCTFLMQFSLSLSSLFVIGNLCVDPVTGSSAKYLSSASLAVMTFSITGLAVIEGLSTSLDTFCSQAFGAQKYTKVGLYTQRCTLMILTALLPVVCAWWISARWLSVFIPQEDLLEDIQDFLRIMSLGIPALTMFETGKRFVQTQGHYNVSTYSLISIFPINLVLIYWLTLKIGYLGAXIAIAISYWLMFFSLVGYCIFIKKDTLKCWAPIFPIEVTNEKNWLKKFGKAVQVLVCGITEFNTDTLENDTVTSNNYGSIAPLQSKEYVQIQKKALPILQHWKPMMELAWPGLVMIESEYLSFEILTIFSTYLGDETEIAIQTVLASVGTLIYQDVFAMGCVISTRVAQHIGSYYNEYTNTNTQSHPNNQTEKLAKQNAVKCIKACYCLGISTGIMNCAILILCAKPLARLFTKDQLVIETAALIMPVIALNQLPNSCHVTNASILRGQGRQKICGVLNLVAYYCLSLPLSHFFAFSLGWGIKGLWIGCSFGLTFLAVSEMWYVVTTDWDYVLDSFIERLHAEHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF54</accession>
    <name>A0A1E5RF54_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Heat shock protein 60, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2010</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the chaperonin (HSP60) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF54"/>
    <dbReference type="FunCoup" id="A0A1E5RF54">
        <property type="interactions" value="1583"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF54"/>
    <dbReference type="InParanoid" id="A0A1E5RF54"/>
    <dbReference type="OrthoDB" id="1733909at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03344">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000001">
        <property type="entry name" value="60 kDa chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00600">
        <property type="entry name" value="CH60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018370">
        <property type="entry name" value="Chaperonin_Cpn60_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001844">
        <property type="entry name" value="Cpn60/GroEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02348">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000592">
        <property type="entry name" value="PRK00013.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009487">
        <property type="entry name" value="PRK12849.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009488">
        <property type="entry name" value="PRK12850.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009489">
        <property type="entry name" value="PRK12851.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45633">
        <property type="entry name" value="60 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00298">
        <property type="entry name" value="CHAPERONIN60"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00296">
        <property type="entry name" value="CHAPERONINS_CPN60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="546"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="553"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="566" mass="60038" checksum="EDAC60E0E9C8FCC2" modified="2017-01-18" version="1">MLRARANIVRNLRHYSAGHKELKFGVEGRAALLKGVEILADAVSATLGPKGRNVLIEQPFGSPKITKDGVTVAKSITLQDKFENMGAKLLQEVAAKTNEAAGDGTTSATVLGRAIFTESVKNVAAGCNPMDLRRGTQAAVEKVIEYLSANKKEITTSEEIAQVATISANGDHHIGKLLASAMEKVGKEGVITIREGRTMEDELEVTEGMKFDRGYISPYFITDTKSAKVEFEKPLILLSEKKISAIQEILPSLELSNQTRRPLLIIAEDIDGEALAACILNKLRGQVKVCAVKAPGFGDNRKNTLGDIAILTGGQVFTEELDLKPENATIAQLGSCASITITKEDTVVLNGSGDKSNIQARIEQIKGSIDLTGTNSYEKEKLQERLAKLSGGVAVVKVGGSSEVEVGEKKDRYDDALNATRAAVEEGILPGGGTALLKASKVLDEVKVDNFDQKLGVDIIRKAITKPAKTIIENAGEEGSVIVGKIIDEYGADFYKGYNSAKGEFCDMLAEGIIDPFKVIRTGLVDASGVASLLATTEVAIVDAPQPPAAQGGMPGGMPGGMPGMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF70</accession>
    <name>A0A1E5RF70_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Transaminated amino acid decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2403</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TPP enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF70"/>
    <dbReference type="InParanoid" id="A0A1E5RF70"/>
    <dbReference type="OrthoDB" id="308383at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004737">
        <property type="term" value="F:pyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000949">
        <property type="term" value="P:aromatic amino acid family catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02005">
        <property type="entry name" value="TPP_PDC_IPDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07038">
        <property type="entry name" value="TPP_PYR_PDC_IPDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000024">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012110">
        <property type="entry name" value="PDC/IPDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047214">
        <property type="entry name" value="TPP_PDC_IPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047213">
        <property type="entry name" value="TPP_PYR_PDC_IPDC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452:SF3">
        <property type="entry name" value="TRANSAMINATED AMINO ACID DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036565">
        <property type="entry name" value="Pyruvt_ip_decrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="10">
        <location>
            <begin position="12"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="9">
        <location>
            <begin position="456"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="497"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="524"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="526"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR036565-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="620" mass="70768" checksum="8F245DA5DE82ACCD" modified="2017-01-18" version="1">MTEETRITGTSTVGKYLFKRLAELTKTIFGVPGDYTLKLLEHIYTFDEKDMKWCGNNNELNAGYSADGYARINNGFGCLITTFGVGELSALNAIAGAFTEHVGVLHIVGTTSTMVKEIDNSSMFDTLHHLVPNKKTYGKQDQYVYEKLVDSFSCCTESLTLKDCERGSNRVQMKIDNVLEQIITQMKPGYLFIPCDMSEMEIDVDFDKKLQFSTNLQLPLSPKETEVVDFLLDETYKAQNPGFIGDLFLKREQAAAMDLLTKTNWYCYSTPCARGHVVDESLANFQGVFSNEFSTANTLEIMDSNDFIMHWGSNINEMTFKKRGDLNHXWLSKLNIIFLGKDFVQIGYGENSRLFTDEEGSLNCVKVFKEYYKRLDFTRLPFIKTRSESAKDLVVPEWSSSYKTDISCKTDAILTQEGFSKMFCSPGFLQKNDVIVNELSSFIFEMGNIRLPKGVKTYHQGFYGSIGYALPATLGVCLAIKDLQLENKHRVILVQGDGSAQMTFQELGCYLAYNIHPNIFMINNSGYAIEREIGGRHRSYNDNICDWDWCSMFKAFGDKQSLKHYAEKITTFKQLAEVNKNVVQNSDYENKVKMIELIFDKNDVCPTLRKYMELKRKMKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF72</accession>
    <name>A0A1E5RF72_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">t-SNARE VTI1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1750</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VTI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF72"/>
    <dbReference type="FunCoup" id="A0A1E5RF72">
        <property type="interactions" value="894"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF72"/>
    <dbReference type="InParanoid" id="A0A1E5RF72"/>
    <dbReference type="OrthoDB" id="430637at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15862">
        <property type="entry name" value="SNARE_Vti1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000002">
        <property type="entry name" value="Vesicle transport through interaction with t-SNAREsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.400">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027027">
        <property type="entry name" value="GOSR2/Membrin/Bos1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038407">
        <property type="entry name" value="v-SNARE_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007705">
        <property type="entry name" value="Vesicle_trsprt_v-SNARE_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230:SF26">
        <property type="entry name" value="VESICLE TRANSPORT THROUGH INTERACTION WITH T-SNARES HOMOLOG 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230">
        <property type="entry name" value="VESICLE TRANSPORT V-SNARE PROTEIN VTI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05008">
        <property type="entry name" value="V-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12352">
        <property type="entry name" value="V-SNARE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028865">
        <property type="entry name" value="Membrin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="10">
        <location>
            <begin position="128"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="225" mass="25320" checksum="6AF96BAFF16333EB" modified="2017-01-18" version="1">MSTLIETYESEFQTTFDRAKSLLSSATSSTTGNSNALLKQIEGFKDDLMDLIQQIELEVNSPNASPQWKIQMKTKLRNYKQQVINEIQAPLNEMVSNDKRQQLFGAASGADADGDYTGDSVSNAQRQQLLGTHQQLQNSSNKLRDATRVANDTENIGSQIMMDLRSQRETLENARQTLFQADSYVDKSIKTLKVMGRRLIANKFISYAIIAILILLILLVLYSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF80</accession>
    <name>A0A1E5RF80_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Endonuclease III homolog</fullName>
            <ecNumber evidence="6">3.2.2.-</ecNumber>
            <ecNumber evidence="6">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase</fullName>
            <shortName evidence="6">DNA glycosylase/AP lyase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">NTG1</name>
        <name evidence="9" type="ORF">AWRI3579_g2248</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the Nth/MutY family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="6"/>
    <dbReference type="EC" id="4.2.99.18" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF80"/>
    <dbReference type="FunCoup" id="A0A1E5RF80">
        <property type="interactions" value="499"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF80"/>
    <dbReference type="InParanoid" id="A0A1E5RF80"/>
    <dbReference type="OrthoDB" id="3970672at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000703">
        <property type="term" value="F:oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03183">
        <property type="entry name" value="Endonuclease_III_Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030841">
        <property type="entry name" value="NTH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43286">
        <property type="entry name" value="ENDONUCLEASE III-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43286:SF1">
        <property type="entry name" value="ENDONUCLEASE III-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3 6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="8">
        <location>
            <begin position="127"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="406" mass="46326" checksum="F3A08B34607460D0" modified="2017-01-18" version="1">MKRKRIEVEIEEPAGSENEVMLKYQAHVQKIKSLQITEYLKLMTRELTLMEPLETLLEQDPAKAAKLPKNFIPIYSKIREMRKTVSAAVDVQGCASMPFYLNFKLPHEPIWPTPENFRLQVLVSLMLSSQTKDEMNCKAMSQLMVYCLDNLDSAHGISFKSLSAIDEHTLDTLIQPVGFHTRKAKFVKNAIEAIISQHATSSLNQDSPLDVPYLYDDIVALQGVGPKMANLLIQCAWAYGEHGIGVDVHVDRLSRMFGWVPGPKERKNPEETRVALQKLLPVSLWTEINSVLVGFGQSICIPRRQKCNECFANDICPGRIRSKIEKTYSSYEEWFSETKNMLPIRTVEGEIEDMVDFVKKDIKMEEDETNPGVKPEPVPFNFSSDKHPVVEKKLTTSKYFNKKKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFL2</accession>
    <name>A0A1E5RFL2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sphingoid long chain base kinase 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1844</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFL2"/>
    <dbReference type="FunCoup" id="A0A1E5RFL2">
        <property type="interactions" value="611"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFL2"/>
    <dbReference type="InParanoid" id="A0A1E5RFL2"/>
    <dbReference type="OrthoDB" id="3853857at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001727">
        <property type="term" value="F:lipid kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050187">
        <property type="entry name" value="Lipid_Phosphate_FormReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045540">
        <property type="entry name" value="YegS/DAGK_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358:SF31">
        <property type="entry name" value="ACYLGLYCEROL KINASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358">
        <property type="entry name" value="SPHINGOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19279">
        <property type="entry name" value="YegS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAGKc" evidence="6">
        <location>
            <begin position="300"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="529"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="530"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="727" mass="80760" checksum="53F5CB2B385C7E8C" modified="2017-01-18" version="1">MEYPNQSNTYTPQASPSLNKETIKDSSQSLTDMKFTNKAGTQNLKKGNLNISRNAINNLAYFTNKGILIKNEVSENANRAAGSATRSAMSGSRSNRLDDGAALQDDYEHGVEYNGVDSEEEDGRDAYETTSLISCVTCLSGHSLSTLSFADSQTGYGDDNSRLLSSSSSSNSVYTDALTGEEDAHGVKTLINDFKIKSVHLPNNSVIPFSKVLNCKQVVDTEVGSTVYDSTGIQEPVHLIEITFAKDRRQDLVPKSSVLKVTHAEYSKLLKEYQSFNKSTIVKELSELVLWKSFPQGEENRPKKILVIINPKGGKGKANKLFLTKARPILLASGCLVDYKETTYHREAYHFTKKYPFLSKYDVITCASGDGIPHEVLNGLYKRKHDRVELFNKISLTQIPCGSGNAMSVSCHGTSNPSHAALNLLKGRPTKTDLMLITQPSYFTNEHGIDDDDEEPHRVSFLSQAYGIIAESDINTEYLRFMGPSRFELGVTSCVLRRKKFPCEVFVKYGSKTKKELRDHFNKHSRYVQSFPNSSSSSPSLPFSSEEDRSLVDIDNPSEGTNSTSKNAFELLTEDDFKPEYGFPENSIKYTDDIPDDWEQIDSSLTKNCGIFYTGKMPYIAEDCKFFPAALPDDGVMDLVITDARTSITRTASILLSLDKGSHVLQPEVVHSKVKALRLIPHVTKSVISVDGENFPLEPMQVEVLPRICKVIMLSNGNFVDTKFDSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGI0</accession>
    <name>A0A1E5RGI0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Beige protein homolog 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1522</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">May be involved in protein sorting and cell wall formation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ85987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGI0">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGI0"/>
    <dbReference type="InParanoid" id="A0A1E5RGI0"/>
    <dbReference type="OrthoDB" id="26681at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06071">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1540.10:FF:000001">
        <property type="entry name" value="neurobeachin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1540.10">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036372">
        <property type="entry name" value="BEACH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050865">
        <property type="entry name" value="BEACH_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023362">
        <property type="entry name" value="PH-BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743">
        <property type="entry name" value="BEIGE/BEACH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743:SF123">
        <property type="entry name" value="PROTEIN FAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01026">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81837">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51783">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BEACH-type PH" evidence="6">
        <location>
            <begin position="1269"/>
            <end position="1406"/>
        </location>
    </feature>
    <feature type="domain" description="BEACH" evidence="5">
        <location>
            <begin position="1453"/>
            <end position="1747"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00054699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00073334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="2041" mass="234774" checksum="60281F4A286C75CA" modified="2017-01-18" version="1">MHSATLFANLCKDFFDFTPNDSELKSDDLHQDFKYKPETASLILQQLNHHQTTQTCLSNEIPQQQEDQSLEYELLYHFKPIEYFDANLKVPKIIPSLEDEFDELVKNMKYTPIIRIILHLITKSVTNRYILNAYYGTTLIPKLKNQLAKDHCKYRFALLIEIISADCKPFKLFNYQEQSLSKLDFYALLELSKVDPLNSYVLLKSCSFCFSTKLHNGENTVVSKIKFLNIKTPIRVFSFHDTSLRYGSEVCIDVSESGIQIYEDSHANFYEYEFLDNKSYTFTFLFDXRAKKLSLYIDLEEICSFNMLIHTHGVSTTLGHENNTGLLLLFDIKFFKTLLPKSYISLYANHKTNYKTQAALYDLPKNCKRNFLVASSHHTAAEIFNSNEDAIDLSMGRRSDKQMMYFSLFRIPHCLLNWGLFPHVIYRFHCASAKELFQELLPLFLNKTLSNSYMEQRFREFDGYLTLTHLIMVKIRDYTNKTEVVNDLFKALSGQDNEVILNENLFTQLVLPILRWPNADEKVVNTFLLKLHFFFTDSNRYSAHNIQVLENENTVSLILESLSLHEENFEHFKNPFTECMTSILSNPFAEKSFNILFFFTYHKLKFQEHSALAFSVLSILDNVFCRALDTLNKPLLQMIKKCWSLKYLLMLFNASFQSKCFLEAPLYCVSLLLRFLELDENDKLFFQNDAFAVMFAIIRRLDPTLKVKVVQMLYVASFETNLQGKTFPFSDDFNKLENGFVREQTELHLLALKIISFEIVQHEFCSAAYIDAISQLAGNSQKYRTFLQKEDARVLIALIKLLRLMKNAETKVRLELLLASIMIDGLASLSNSELVIFSKHLNFSISLAVPVTTKSTKCTILVNIIPKIVERLTQFESSFQIFCWENKDFVKNLLYFFQVVDENILLLDWDLKFLMDVLKVSMVIIEFKKNEWTSSHKELKFMYNLVSHCLLSSIKAAQRLPSPPNVIEFFKLLLTYQETLFSKNNGARVTDTVFVPLLSFIVCFSATENVSDPYSVTSFRTILMHKEPELKELSKQFCRNCKAFPKDFLECSLTANDDELFKMFKAQQSLFAEALDADLQAKRRHSVTRSEQAKCLLEMEKEATIYQENFIKKDIQYSDQVYELFKKDIAPFEAKALYAERRRQSSIKDDQEEFSQYYWQKILNIEYDITTMYKLYGAQKPSENWQRYGLSASSTELLKKTLGLEFKRNSLQMESFYDVIAPCANSETFKASSAQVENADMVETTSNLQTSEHTLNIPNENRKVLKNLLPEETISQIWNSSMVIGLHIEEGVFILGEKSCYFITNFFYSKAGKKIIELSKANADERDDIIQLVSGENNTSFPNESYSGKEKLHEVTKFAVNEIACVAKRPFLFQDQAAVVIVQSRKSFFFSFDSSSSRDSFYRKLSQIQNPSILEDKVLDLVFKHINSQNETTTVRNGISSGSFTWKLTNAVQSLAESSSLESITEMWRQRKISNFFYLIIINLFAGRSFNDLTQYPVFPWIIKDYHTNNIDLSNPASFRDLSKPMGAQSIRRANEFIERFEALEELNDPSITPFHYGTHYSSAMIVASYLIRVEPYTASYFILQGNKLGPSDRLFNSIERAWKSASAEISTDVRELVPEFYYLPEFLVNVNNRDFGTLQDGSKVDFVELPAWAKNDPMIFVRTNREALESDYVSDHLHEWIDLVFGFKQKGQEAVKAVNVFNELSYSGAVKLDNIRDRREKQTLTSIIHNFGQTPLQIFETPHKQRSNGPTEPQTLLAGSTCKVNTAATIRNRLCAKYLFPRLINDTSAARVFFELNIDENAKSKFKENKFWLNGVLGVSNEISFKALVKIRANVFATGDELGGIIVWNLEKGIAVAQQTLCGHLYSIMDMDVSPENNVMISLDTSGKLLQWDLSSGGIIAGMEKSASIKNIAISNASGNVAISKNCSVTLLNINRRQYLTADLPYMPTAINFCDEGVLTHAFSSFIDILIFGYENGIVEVYALTLEASCWQLRLVQKYQTEESSAIEAVGGSKTEDLKKNNVLLKISAMDANGKMYTWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGP0</accession>
    <name>A0A1E5RGP0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Rab guanine nucleotide exchange factor SEC2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1583</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGP0"/>
    <dbReference type="FunCoup" id="A0A1E5RGP0">
        <property type="interactions" value="220"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGP0"/>
    <dbReference type="InParanoid" id="A0A1E5RGP0"/>
    <dbReference type="OrthoDB" id="1748564at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070319">
        <property type="term" value="C:Golgi to plasma membrane transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21044">
        <property type="entry name" value="Rab11BD_RAB3IP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040351">
        <property type="entry name" value="RAB3IL/RAB3IP/Sec2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009449">
        <property type="entry name" value="Sec2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14430">
        <property type="entry name" value="RABIN3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14430:SF0">
        <property type="entry name" value="SEC2P DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25555">
        <property type="entry name" value="RAB3A-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06428">
        <property type="entry name" value="Sec2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144284">
        <property type="entry name" value="Sec2 N-terminal region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GDP/GTP exchange factor Sec2 N-terminal" evidence="4">
        <location>
            <begin position="46"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="429"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="587"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="651"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="75"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="190"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="439"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="683"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="695"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ86044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="747" mass="83293" checksum="706D4E7C9F41AFDF" modified="2017-01-18" version="1">MSHGKQEAVEEVIAADPINHADNARLEEKLNAMSTQLLESINKQSSFETKIFELQKQITVFQKQEEKYVSFEQDLLDAKRLAEVQEKKISDLQKELSKEQAYKATAEQEANGLREEIGELSTSLFEEANKMVAAEREKLYYKDKELISLKGVLQEKEDILQSLEMQLKNLKVLLEADASNDDLVKESSVEAGSTPTNTGSLEKSVSKNSMPASVNVDAIFSPTLQSVRFDTEMYSEFLKFIAVLPHNDSIYSTKNDSKLIKKLLKEEIEPALKIDSAKGIPWAARSRIIPAMMAGEVAIEPFSGINETFRYNTNSSSNPKKSDSLSDTKMFKYPKDSPPVAVDEPCSFCFESRSDILEHNRLYCLKIFSSRDPSVVSKQYPLCHYCLLKIRQCCEIFAFLRSLKDGVWNLEKVTLKDLSKSETSELFSGYNAPNAPEATKTSTDTLSRKPSLSNNDLNAQEKRNRRKSLIDSFSYLSTGPVLEPEIQVNKDLATISVSVDGHAFLPNNNIQRSWIQLCKLRAALYWCIVGIWSVDDCVSAKIVPFSKSAMNNLASPTLAKNSSPTLSKLSEASNSLSQVSNLNNNSIKESVEESPNEKPSTGKLGIKGNQDNLEGNDRSDTTVQVFAKNAPEELEDKDIASAEQFDFEEPAAAASSLKEQGNIDQSHELDDQGSAHNVSTSELDEHNLKVKSSDEEIYFDSIDEDNNANDAITPTNKDKKSRSSRHMSDLYKNLDSLAQDVETAFKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGV2</accession>
    <name>A0A1E5RGV2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1490</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NPR/HAL subfamily. HAL5 sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGV2"/>
    <dbReference type="InParanoid" id="A0A1E5RGV2"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF43">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE HAL5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="737"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="20"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="131"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="249"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="454"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="558"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="618"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="697"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="792"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="29"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="62"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="84"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="133"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="249"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="302"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="378"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="457"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="473"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="567"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="630"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="641"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="792"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="805"/>
            <end position="825"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ86104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1128" mass="122690" checksum="BA6517FAB6F6EF27" modified="2017-01-18" version="1">MGNTEVSDVKRTRSLSKSIKNLFTGGSNGNNTNNTNNTSNNGSRSAESPGNNKEELKLKRNMSMNKPNLKVNTTANESKETEMNKGNSSARPSRKNSVTSVKDVPMNHIDSFMTTPNEDTQTGTANVMAPKENIGISGSSSINSNSNSNSNSNSNSXSNSNSNSNSNSNSNSNSNRNSNISSSSSSSSSSSSISSSSISSSTANSPIVKPRAVKPGNIVAAQTLLTGKHMHPQEVESLVGEGSKENFYKDKFKEGGKEGXKEGGKEGGKEGGKSNGSSDMLFMKSSPATPRAGTPVRRRRGNSNASALSQENGTNLYHYNHTHTHPNQPQQQTMGSNMNNNNVPPTQANTPPATLHGPRRSNTISSNAFYPHHLSQDTNNNNGNSSNLTSNNSSSTSVNNTNNASQLMLNQIQNQPVIYKSDSFVLFKNFMHEHHLTVVPLVTISSTAKGAVTNTVSPTSSNAGTASTNTSPGSATKNSNGVNEPSKLEKINSNGSVNKMEKSPIXAGTSAGEQQEPHQPVPQSHQNIKLPKNTSFSITSLFKVHKNDSHMGLPGLNNTNGTVNTTDPNHQQLDPQHQEQFQYHSSSWYSLSKKEVCGNNKFNTNIKYAKSLMNNESSYRRAGPTTGSASGSGSGSGSGSGSASSSNSSSSSSAAAPPGLKNPQTPNIVNNKAAISEEELNLINDLTIKMKKSIKNDSLSNKNNYNTNARSPLSNNGETNSGSTEYTVDNVFFKKYGLCIGTLGQGAYGVVKVTSKKLISSKPTMAQLDTNCKKTFRYKDSAFFAIKELRPLRPDGDRKDENESENTTDSTSASSTTNKNSSSAAHNEDGTVRTSADYDEKFCTRLTSEFVIGHALNHVGRHPNILKVLDLFTFTNMPNKFIQVMEFCPSGDLYSLLKSHPNKLHPLEIDCFAKQLINGVSFMHEHGVAHCDLKPENLLFKPNGILKISDFGTACVFQTAWEKKCHLQTGCMGSEPYMAPEQFIPRKQYDPRLVDTWSIGMIYMAMCLGHYLWKSPLKDKDENYNEYLESIDYFKTAKHEAENENNKKGSSDSLVELNASIKSGQDDFGDYSVFENLKHVNSEIKLARKACLYKMFSPSPAKRIQVKDLMYTTWMKKTHCCVVYRNTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGV8</accession>
    <name>A0A1E5RGV8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial intermembrane space import and assembly protein 40</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Mitochondrial import inner membrane translocase TIM40</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g1229</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
            <orientation evidence="2">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGV8"/>
    <dbReference type="InParanoid" id="A0A1E5RGV8"/>
    <dbReference type="OrthoDB" id="7481291at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045041">
        <property type="term" value="P:protein import into mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010625">
        <property type="entry name" value="CHCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039289">
        <property type="entry name" value="CHCHD4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012891">
        <property type="entry name" value="GCK_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21622">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21622:SF0">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06747">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01227">
        <property type="entry name" value="GCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="GCK" evidence="13">
        <location>
            <begin position="312"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="87"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="117"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="146"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="183"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="212"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="266"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="290"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ86125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="462" mass="50064" checksum="521487E6D56C29B4" modified="2017-01-18" version="1">MFRTSNGLKRVALSAFKVQNKRLANTNNVSLLRNSSKRFGSVRYYSAGSGFHAEPEPVAYKMQGIALAAALSGVALYMVLPRSNKKPVEIDNNNNNNNNNASNESQSDGPGVKPETPSEQEQGQTEDSLKNDSNDESDEQPEESDKEXKEEKSDKESDKESDENIEHEQNQEEDKEADQNDSAAKEDAKDSSDEKPEKNEQSAQDEASQESKASKPDSEKVGKVEKNDSEQQTTSTKESEDLVEVVTPAGDEILIADGPKTGEQPPADKKSKDEPQAAPXVPSTQPANEAKSENEPEKREAAYNPETGEINWDCPCLGGMAQGPCGEEFKEAFSCFVYSEAEPKGIDCVEKFQNMQNCFRANPEYYADQIKDEEEAASAASAAAAAAVEEELSAADVPVAVPAPEEIVIEESNISNNPTLVVDEIDVESADTNPQGPEIIADPVPAVETPEILVEETGSKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGY4</accession>
    <name>A0A1E5RGY4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Bifunctional purine biosynthesis protein ADE17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g1487</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Bifunctional enzyme that catalyzes the last two steps of purine biosynthesis. Acts as a transformylase that incorporates a formyl group to the AMP analog AICAR (5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) to produce the intermediate formyl-AICAR (FAICAR). Also catalyzes the cyclization of FAICAR to IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(6R)-10-formyltetrahydrofolate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + (6S)-5,6,7,8-tetrahydrofolate</text>
            <dbReference type="Rhea" id="RHEA:22192"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="2.1.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:18445"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="EC" id="3.5.4.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PurH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGY4"/>
    <dbReference type="FunCoup" id="A0A1E5RGY4">
        <property type="interactions" value="1065"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGY4"/>
    <dbReference type="InParanoid" id="A0A1E5RGY4"/>
    <dbReference type="OrthoDB" id="6017153at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00133"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004643">
        <property type="term" value="F:phosphoribosylaminoimidazolecarboxamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01421">
        <property type="entry name" value="IMPCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.20:FF:000003">
        <property type="entry name" value="Bifunctional purine biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1380:FF:000003">
        <property type="entry name" value="Bifunctional purine biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.440:FF:000001">
        <property type="entry name" value="Bifunctional purine biosynthesis protein PURH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00139">
        <property type="entry name" value="PurH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024051">
        <property type="entry name" value="AICAR_Tfase_dup_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024050">
        <property type="entry name" value="AICAR_Tfase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002695">
        <property type="entry name" value="PurH-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005492">
        <property type="entry name" value="PRK07106.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00355">
        <property type="entry name" value="purH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692:SF0">
        <property type="entry name" value="BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN ATIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692">
        <property type="entry name" value="BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN PURH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01808">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000414">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00798">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MGS-like" evidence="13">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ86160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="596" mass="65759" checksum="2B2C4A6F43E9F6ED" modified="2017-01-18" version="1">MAYKKTAIVSVYDKTGLLDLAKGLQENNVRILASGGTAKMIREAGFQVEDVSTITHAPEMLGGRVKTLHPAVHGGILARNLDSDEKDLQDQKIEKVDFVVCNLYPFKETVAKIGVTIPEAVEEIDIGGVTLLRAAAKNHSRVAILSDPKDYPQFLQELSADSNNEISQNLKNKLALKAFESTSDYDTAIADFFRKQYSENESQITLRYGANPHQKPAQAFISQPYDQLPFKVLSGSPGYINLLDALNSWPLVKELSGSLNLPAAASFKHVSPAGVAVGLPLNDVEKKIYFVSKIENINKSPLACAYARARGADRMSSFGDWIALSNVVDLPTAQIISTEVSDGVIAPGYEPEALALLQKKKNGKYCVLQIDPNYVPDALETRSVYGVSLQQKRNDAVITQSTFKEIVSQNKHLPEQAIIDLTVATISLKYTQSNSVCYAKNGMVIGLGAGQQSRIHCTRLAGDKADNWWFRQHPKVLAFQWAKGVKKPEKSNAIDLFVTGQIPTQEPEKQEYESKFSVVPEPLTEEERKEWLSKLNNVALSSDAFFPFPDNVYRAVRSGVKYIAAPSGSVMDKQVFGAADSFDLVYVENPFRLFHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH11</accession>
    <name>A0A1E5RH11_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Sterol esterase TGL1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1480</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH11">
        <property type="interactions" value="1384"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH11"/>
    <dbReference type="InParanoid" id="A0A1E5RH11"/>
    <dbReference type="OrthoDB" id="9974421at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000095">
        <property type="entry name" value="Triglyceride lipase-cholesterol esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="9">
        <location>
            <begin position="125"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="474"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="476"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="553" mass="64334" checksum="3CF2394803A10485" modified="2017-01-18" version="1">MVPFIGRLSMTEWILLFLVYSERICSFFINNLIPSPLIRLLQALADTIMNLISLLIPRSSIGKTRVESEKDKLVDLLKHSASIKETIHYLNPXITIEEHVVVTEDKYLLTLHRIPPDPTRECKGVVYLHHGLLMCSDIWICHHIDPMTKNLPLLLHSLKNYDVWMGNNRGNKYSTYNLNYTINDKKFWDFSLDEFALFDIPNSIDYILKTTKKSKISSIIAFSQGSAQTFGALSLHPELNEKVENFIALAPAMTPPGLYNKIVDTLMKTSPQLIYLFFGKRILLSSANRIWKKTLPTRLFNMGIEMSTKFLFNWNNLNIKQARTIAFNNLYSPTSVKCVVHWFQILNAQKFQMFQEISFLGSTSGGSQQTNLFHPLCFPTLTNVKIPVLLIYGGSDSLVDIEVMKKNLPKELCFQVEIPKHEHLDIIWGDHCHTKVWPHVLKFMQFWNEDFKKSYENELLVEEDELDRQAPAKHLLSHEPHQQNREKDXRHRRHASNPLMHQPVRRNSDYFLPFDHETEDVNKYFAREDVTPTLVSTTEYVDLRQHLSDRTLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHJ4</accession>
    <name>A0A1E5RHJ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinetochore protein Spc24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1477</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHJ4">
        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHJ4"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="24462" checksum="F31B2C2FB37E1829" modified="2017-01-18" version="1">MSITVGTSQNIKSMEDAVQALIEVRQNINFTDIERVAQVKHRAEALKKTQEEDITKLSAXNSTLKQEHDDVLVALQTLQLSDNGQRQRGNNDSADNAEDKRLDLEIEEYTEKIEALRASIDDMNSDMDKGLEEIARLKTVKEDIVLTKQRNNDNYSKLQIYKMLGLLLDSENMDLYIKEDEVINIQEILNLPENSRSEKREEIWNLISGTQEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHP0</accession>
    <name>A0A1E5RHP0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RNA polymerase I-specific transcription initiation factor RRN3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1451</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRN3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHP0"/>
    <dbReference type="FunCoup" id="A0A1E5RHP0">
        <property type="interactions" value="672"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHP0"/>
    <dbReference type="InParanoid" id="A0A1E5RHP0"/>
    <dbReference type="OrthoDB" id="26970at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001042">
        <property type="term" value="F:RNA polymerase I core binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001181">
        <property type="term" value="F:RNA polymerase I general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007991">
        <property type="entry name" value="RNA_pol_I_trans_ini_fac_RRN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12790:SF0">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12790">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IA RRN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05327">
        <property type="entry name" value="RRN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="256"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="256"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="298"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="667" mass="76646" checksum="327223C76D4E48C6" modified="2017-01-18" version="1">MLASKRAHADNEGSSNGSASKKIKKNVTFDLPSGNQIKEKSGTNPANAAKIHYKYMQSAIDEADQHNDSTAIENLILQIEHHDSHKDKIKPKTFEILLSVLSNNISKVENNSKFNSLVISIINYNKWYKIPSETCVYEYMTFLKILCSSLPKWLAEVLGMLISQLSEVPIKESTYRHHVLMQYFVKVFPTSVNLFTANLSKNFPNKNDTKTRNVNYIENLLKITEYCPEFKFPIWSLIVEKAISIDVELQNELDDLDDDIDALDSDEDDDDDDEDDDDEEDDDENDGEDVDADARDNDAEDGGEEAFMDGDEEYQVELSTNIADLSGKLDYILSVISKNLAHLLDDENLEQGDGINVFNTLMSLFKTHILPTYYTKSIQYLMFHAAQQQPELMDAFLVTLIDISFSPNESMENKIKSLQYVGSFIARAKKLSKSQIMFVSSYLTSWLNRYVIEREIEVDLLPGGMERFKHFYAAFQALCYIFCFRHHYFKVDFTEGKDFAESQSTNDKKSKQANNEESLWECDIDKFFQRMVISKFNPLKFCNENVMLMFAKITQTENLVYCYSIIEKNNSDRLRGIVSANAKGSRNGASLNQSRTVAPIPLADRESGNKGFSLVARQQFIDLQSYFPYDPLFLKKYKRLMHDFYIEWSEVSGDYETDESEEDDDNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHS2</accession>
    <name>A0A1E5RHS2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1655</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHS2"/>
    <dbReference type="InParanoid" id="A0A1E5RHS2"/>
    <dbReference type="OrthoDB" id="4096201at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="493" mass="55859" checksum="6EB6E7546AA98876" modified="2017-01-18" version="1">MLLNKKLFLTPASSPVGSPSPSSSSSSSSSFTPASSKGKYKLGSKDSQQKLSNYKKGHQVSSVLAPSNYYNASYDINDEERTSSTVVNHKRAKTSGDYFTHKHYSESDSSDFNESDSDLDFDNSFTEYSLSFPKLNHKNSISKKSNRCFKANSRPTTPVVASAAPNDKKCLARKKPPHKSVVQQFHAKALDNISTKLNNFTYFIPSKTKSMMESSCTGAAVSNGTPQITNANSFSSSESLMQEMNMKNGKESKYNFYIHCDMDARERCFNYILETIDAVWARYCDATTLVEEEFYPTTAVNSPQISNNKTHKRSVSDIGRESSMDETLDNSTDVLDQKTRRRCSSIVSGKSISMMNNNNTMTNVQTQNDAQNNSATELEVDVNKLNHFKQRLSSTKMYMEDLVDSTDMLDLVNFWKKWDLIKYNCIQFMENDEDEYEDDIYDSSNYVNCNDQETQWSSFTNNKSALKPDSFDLCDEVLNKLEKGRYYYNNDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHY8</accession>
    <name>A0A1E5RHY8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein SVP26</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1053</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SVP26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHY8"/>
    <dbReference type="FunCoup" id="A0A1E5RHY8">
        <property type="interactions" value="274"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHY8"/>
    <dbReference type="InParanoid" id="A0A1E5RHY8"/>
    <dbReference type="OrthoDB" id="28257at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097020">
        <property type="term" value="F:COPII receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007277">
        <property type="entry name" value="Svp26/Tex261"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13144:SF0">
        <property type="entry name" value="PROTEIN TEX261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13144">
        <property type="entry name" value="TEX261 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04148">
        <property type="entry name" value="Erv26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="180"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="233" mass="25996" checksum="C156A098631DF98F" modified="2017-01-18" version="1">MLLQVLSIVGTLCGFVFATLSLASGLYYISELIESKCEPTKKFLSKAIYGIIIAFVLLMLVDRFPIKLCLLSIVSNVVYYQNLKKFPFINLGSIFFIGSIVLVILNHYLWFKYFNNIEIPPQFKYDPKYIPQKRANFAEVASFFGIMIWFVPFALFISLSAADQTLPLTSGSEGSSGVIPSPSGLTSSSGKKTKKMQSIGKVVISTIKEYFELLIGVFSSTQAAKNKKFDRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI10</accession>
    <name>A0A1E5RI10_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphatidylinositol transfer protein CSR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1289</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RI10">
        <property type="interactions" value="196"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI10"/>
    <dbReference type="InParanoid" id="A0A1E5RI10"/>
    <dbReference type="OrthoDB" id="43460at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052432">
        <property type="entry name" value="PITP/CRAL-TRIO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46590:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN CSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46590">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN CSR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="194"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="445" mass="51687" checksum="366D842D1C924622" modified="2017-01-18" version="1">MTSASVSIPSGRVETLDSTQELILKRVWANFLLHWGYKLDAKDLLTEQQQQQQQQQQQHFNAHNSNESSNNSGGSGLGIKKALFGMKKSSSSSNDSPSTKTRSNSLEVVYPKSYIHEALQKLDPVEVKTGFWEFLRTDYPDNLLLRFIRARKWDLDKTMNMITTTIHWRMNEAHPDKVIQRGEKEAFVNNRLPGLQKNLELRKAIIRGRDLKERPIVFVRPRLHHSSQQTESEMEQFALIIIEXTRLFLKEPLVDQATIMFDMTGFGTSNMDYAPVKFLIKCFEAHYPECLGKLIIHKAPWVFSPIWNIVKNWLDPVVASKVVFTKSLKDLEKFINVQNIPECLGGEDEFDLDHFPITDIQEWEKYDSKLKDTQTRDKLLAERKQIAEEFIQTTINWIEAKDKXISQKFLQEKIKQSKLLTENYIALDPYVRSRSQFDIDGTIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI16</accession>
    <name>A0A1E5RI16_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">General amino acid permease AGP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1613</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI16"/>
    <dbReference type="FunCoup" id="A0A1E5RI16">
        <property type="interactions" value="272"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RI16"/>
    <dbReference type="OrthoDB" id="3970526at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF17">
        <property type="entry name" value="GENERAL AMINO ACID PERMEASE AGP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="358"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="419"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="443"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="490"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="525"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="7">
        <location>
            <begin position="88"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="598" mass="65884" checksum="D14DBD52C307B795" modified="2017-01-18" version="1">MSNCSTKSSSLTKENFSSNTEAVSSNDTVAKKPLLNSPFVQNFVDSFKKQEESVESEGFSDLEATPSNELNGFAEKPKLKQKIKPRQILLIQLGTGIGTGLLVGNGAALRQAGPAGLFIGYAIVSSFIYCTFNSISELGISYSQLSGSYNEFSCFLIDRAFGFSVAVIYALHWLALMPLELTTSSMIIKYWTTKVNPDVFVAIFYVLLLIVNFIGGSGYAEFEFVINSIKVCMILGFIIFGIISDCGGSYGNGHSYIGGQNWREPIGAFFGHTKIDHFKGIASSFVYAAFAYSGFESSILTASEQVNPRKSITKAGKMLIYRIVVIYCLSVVMIGLLVPHNSSKLLGNGSSTSSTSPMVIAAANIKGLPHIINAVILLAVLSVGNNSLYCSSRVILSICEQFFPNVPLLTYVDRRGRPLFALIFAAIIALISFVAASPKQTIVFTWLLSISGLSALFTQLSMNLSYIRFRKAMKVQGRSLNEMAFHSKTGIVGAYYACALIILIFIAQFWIALVPVGSYTPDVNHFFQNYLAMVVFVVLYFGFKIITREWHIFIPAEKIDLVRGRKIFNYEQIKEDQLEDKERLKGASFGAKILNFWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI84</accession>
    <name>A0A1E5RI84_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">YL17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1508</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes). uL22 is associated with the polypeptide exit tunnel.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI84"/>
    <dbReference type="FunCoup" id="A0A1E5RI84">
        <property type="interactions" value="1082"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI84"/>
    <dbReference type="InParanoid" id="A0A1E5RI84"/>
    <dbReference type="OrthoDB" id="10254664at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000448">
        <property type="term" value="P:cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.10:FF:000010">
        <property type="entry name" value="60S ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="entry name" value="Ribosomal protein L22/L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_uL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018260">
        <property type="entry name" value="Ribosomal_uL22_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005721">
        <property type="entry name" value="Ribosomal_uL22_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_uL22_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01038">
        <property type="entry name" value="uL22_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593:SF10">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="Ribosomal protein L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00464">
        <property type="entry name" value="RIBOSOMAL_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6 7" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00062111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00082773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="184" mass="20611" checksum="D0412FB1261FD6D2" modified="2017-01-18" version="1">MARYGAISTNPTKSASARGSYLRVSFKNTRETAQAINGWDLTKAQTYLEQVLDHQRAIPFRRFNSSIGRTAQGKEFGVTKARWPVKSVKFVQGLLQNAAANAEAKGLDATKLYVSHIQVNQAPKQRRRTFRAHGSINKYESSPSHIELVLTEKDETVVKAVEDKKVRLSSRQRGRLAAQKRITA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RMZ5</accession>
    <name>A0A1E5RMZ5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein transport protein TIP20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g907</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RMZ5"/>
    <dbReference type="FunCoup" id="A0A1E5RMZ5">
        <property type="interactions" value="137"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RMZ5"/>
    <dbReference type="InParanoid" id="A0A1E5RMZ5"/>
    <dbReference type="OrthoDB" id="2189254at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070939">
        <property type="term" value="C:Dsl1/NZR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1420">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.100">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.670">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042044">
        <property type="entry name" value="EXOC6PINT-1/Sec15/Tip20_C_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007528">
        <property type="entry name" value="RINT1_Tip20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042042">
        <property type="entry name" value="Tip20p_domB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042043">
        <property type="entry name" value="Tip20p_domC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04437">
        <property type="entry name" value="RINT1_TIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51386">
        <property type="entry name" value="RINT1_TIP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="700" mass="82724" checksum="6E8F399E61321EF8" modified="2017-01-18" version="1">MIESVEAFDKIPELLSDIEQKRVELSSQLQEIEQHNDDLIMREDYLDNFASGSTEAYDLKIQQLTEQLSKIKSLSDLESFISTNKNLQEVSVVKQLKQLLQLQQANENFLRLAEDCNNRIIPQIKTLTEVSEVESYLNTEIFSNIEYTSEEIAFIKKIISEELYGHFLSLKADLTQNFNQLLLETNWDSYKFNNHELIHAMKAKSNQLYQFSILVQEWCPSEPMQYWNFESLANNFKIKFIYHFNNTQQQQQQQQDASQSIETFFSFMETYLSENLLKCIAIFHDEAIGLTKLTVHDQFINYILGPLRKKIKKILTSIVDNQNNLETLVRLITQIFKTDEILWEKYSYSGDGLITLLPNNVLKIWIQFETDIVDKQYAKNFSNYQGLSKIIRDGPNFVKYLLNLYNYFHPFLAVTTEKFFQYKIMVFKKIFLNLLKSFRESNMNYHQSHENDEITNFNENVVRLKNMISVYETMYVLEKQSDCIILNTQFNEITESHYETLFQAEMVEYQEAANALLESLVHRFKKMFGNSLRTYFKVNTWPSLTVTLENDRCSNELIGAVNILSNCYKIVQAHLTGKMNEKYVHRFQLNVINITVDYLMNYIVKLNNFSINGYKQIVQDYNFLKKNIGFEQNTIVSQNDGSLIETLKLYYSLTFEDHAEYPWLNKDYAKFDSNFVRLKRTFNLKYAKNEDISYILYKCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN51</accession>
    <name>A0A1E5RN51_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Anaphase-promoting complex subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g658</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the APC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RN51">
        <property type="interactions" value="253"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN51"/>
    <dbReference type="InParanoid" id="A0A1E5RN51"/>
    <dbReference type="OrthoDB" id="3973130at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007091">
        <property type="term" value="P:metaphase/anaphase transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024990">
        <property type="entry name" value="Apc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12827:SF3">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12827">
        <property type="entry name" value="MEIOTIC CHECKPOINT REGULATOR TSG24 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="370"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1579"/>
            <end position="1610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="378"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1587"/>
            <end position="1609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ88312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1903" mass="216672" checksum="68AD069ADC005B57" modified="2017-01-18" version="1">MSFYGSKEEIEDTSLESLLQKYEKLSEFKYPNDYTVEWFLKSIKYKKFQFHESEKIISVDFVDFENFCNCLVISTKEYAKIINLSNGDIYQTQYPFPTRKVLKYQYGLVLERDMDEDSEHMVNIPWNYKFCAFYDPFGEFGMIEFAEFPNGLQNNLSLLSFPYTKTNDSEKYLATFISSNDTEKRERENPIITSKTNTILYVATVSISKYSKPLYYGNDTVRKNDLSLSSRDPKTVFQHSPTTQNNVTTYDMTNALKKKTPKSYGSLKKNTEQPLSNLRSVSQRGLSARNISDTFNIDINDSNGNNDPLLLNGFEQISSIMNNRIISKNEHDINSTNQTKYHNTSGKKIVSGSSTGLSAGLRNVDLISDNYPPNISENNNNNNNNNNNNNNNNLIMENKIISNNKISDRIYSNVDQNEDINNNLNFDYITAASLSKSFGNLPNQSDLDKKNNLKFVAGKDIDPFPISTLKQSNVEVEKNNGKGNMSYKISTMTNSKKNQNEQIVITLQQIVELSQSFDKTGVSCAHCKNKTREYLWINSLHSEILLKVELNIQSLTSHVEKFISLKPEILVSTKSCDLNSQIVHLTNGFFKIEDTEANLTGKSITIINPLIEFDQTISGRNFSFQMHSKIVTKIFQCIKCLASPKLYCSYLFLWSYVRSLLPSTCSEFDCFAKAMLFVTLNESLYSKKAETIDISSFRLEENKTSILSLYLEITANEEYMKSFLASDIPKLIPSIVLSLSILKEELELNVLEQKNCERLNELLFLLTLIMQWPQAWIEHYQVKVLHLSDVNKSTFEFISKNMRTSNNDSFPSPLLSPNSIIEHIEKMSKPSVATLDSVRLPLSTSNRYLMLSDVFESSQAINFELTPRTCKLIKLFEVFNSASQNANIVQIFKKLQISKHELDTFPLGIYSLLNQLIKNYEKLIVNVNSVIDVDLISRVDLQKTSFSFFHSNTGLSPVSQNFDKTSLNDFDGLANKRKKLPITYHDLLSQITNTFQNSTPSAASMGDTHSVGKFQTANKIFSHDKRLFIASRLICFFLPVLALDYQKIGDDYIQKSSYSYAATLKQLANSVGWGALTYSTCAPLPTEKFELPDFNFSAVYPRDNKVIELPVESVGPSVLHRGQFHSGVASALRISSQTAGLDFNWVSFNKPKKLSVQFGGFLLGLGLLGFLKNLEEWQIYNLLGSKFAPVSVGMLLGMCASARGSMDLMLNKLICVHVDAFLPQGSNDMNISHEVQTAAVLGSGMLFERSFDKRITELLLNCLHGFVRNDATSKKVANEGYRLGAGIALGLVNLGNTRKRQNKETSFTTNHGELVSDATISTLLNTLRLDNDYENELMIANSFIGSTVSLMLIYLKTNNFEIAKTFKKDTFQKSSNAKEISTVRPDFYFYQELAYNMIMWDRLDEHILLTYLISFLKSDTLTIGKELIGLHVLPAYYQLAGFLISYALKNVSTNDATFLRYVLPILDKLISIPAESFGLNYNSVVFVENMNQIKQLIMLSFSLVFCGSGNLEVFSRLKFVHESTLSFSNVLLNDDIPEEFFQNSNSFPNGNTLHGKAQVDFYFETLHKFFEGEQEKKDRQETYYREQQQQHQQQQQQHQSQQGQHSASSNEYSNGNLFVDEIKSKTSTHFSKFSCSSMAIGWLFLGCGQCSFNTTDLEPLAYLLISLLPFSSRMPFELQETRHFYSLAVETRCLITKNCETNKVINEVPIKIAFHDKLKKPQIFQTPFLLPPISSIKQIKVISTDYYFLSIDFDDEIALKHKKDENMIIFLKPRTVDERTAKTLSKEKIKFPLDSTNNQTKAGIEANGIKNVGLYLKNKRRELFDSYNSATKETHLKSTPDVLTKFSEFAKSNDGSSFLKLEFEKDLLAEKRYKASQDKNLDLVALFEDTNYQMELWKSRHDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN57</accession>
    <name>A0A1E5RN57_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Alpha-mannosidase</fullName>
            <ecNumber evidence="3">3.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g879</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Degrades free oligosaccharides in the vacuole.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.</text>
            <dbReference type="EC" id="3.2.1.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 38 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.24" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN57"/>
    <dbReference type="FunCoup" id="A0A1E5RN57">
        <property type="interactions" value="274"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN57"/>
    <dbReference type="InParanoid" id="A0A1E5RN57"/>
    <dbReference type="OrthoDB" id="10261055at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009313">
        <property type="term" value="P:oligosaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000004">
        <property type="entry name" value="alpha-mannosidase 2C1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.110.10:FF:000002">
        <property type="entry name" value="alpha-mannosidase 2C1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.30:FF:000001">
        <property type="entry name" value="alpha-mannosidase 2C1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.50">
        <property type="entry name" value="Glycoside hydrolase family 38, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054723">
        <property type="entry name" value="Ams1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041147">
        <property type="entry name" value="GH38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011682">
        <property type="entry name" value="Glyco_hydro_38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037094">
        <property type="entry name" value="Glyco_hydro_38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028995">
        <property type="entry name" value="Glyco_hydro_57/38_cen_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46017">
        <property type="entry name" value="ALPHA-MANNOSIDASE 2C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46017:SF1">
        <property type="entry name" value="ALPHA-MANNOSIDASE 2C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09261">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22907">
        <property type="entry name" value="Ams1-like_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17677">
        <property type="entry name" value="Glyco_hydro38C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07748">
        <property type="entry name" value="Glyco_hydro_38C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00872">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88688">
        <property type="entry name" value="Families 57/38 glycoside transferase middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 38 central" evidence="9">
        <location>
            <begin position="582"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ88315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1083" mass="124181" checksum="00A0DE0A2914D67D" modified="2017-01-18" version="1">MTTPNNNPNFAQIKSLYEGRLNQFIDGNGQYAHLNLPKFYDIFRKSALRDIINDEKDDDAIVDVTYYQVPYNKENPVSPENRPAWKDIVAADRDGSLKFAKVNDNQPFGPSWTTTWFKVHLQFPTFAQPGETWVFEFDCENEGLVIDGETLLPVTAFSGKAERSEYLIKIPDFDSDSLMKKNYKHFFYIECANNEMFGCGFPGQIDPPDNNKYFHLKKSDLVLPNWEAKKLAYDFLQCQDAAKQLPDDSFEKHLCRRVCNEVMDLFDPNDQSSIAQCRKLIKDSLLGDVDDSTVYNKKNHSQEYVPIFGFGNCHIDTAWLWPFAETKRKIARSWTSQVAIIEQYPEYQFVASQAQQFKWLKLEHPEFFHEKLIPKIKEGSFVPIGGSWVENDTNMPSGEGLSRQFFFGQRFFMKNFNIKSKIYWLPDTFGYTSTLPQIIKLSQMDYFLTQKLSWNNINKFPYNTFEWQGIDGTQVLCHMPPADTYTSDASFADTMKSSKNNKSGDVIGGNLLLYGIGDGGGGPTTGMLEKLRRFRGLNNKLGSVIPKVSLGKLNGVEEFFANIESKNRGKTLPTWIGELYLEFHRGTYTTQAMLKKLMRLSEVQILELEKLCCWWSFENPDFNYPHAKINDFWEKVLLCQFHDVLPGSCIGMVYDHEAVPMLKKVVKDVGKLMTEVLQQANGTYKFNAKTLDFTKKCSTFAAEDDSNITLWNDDLKISIDKQKGVIVSIQDNQYEFLDLKTGRNTVGANQFVLFDDQPLNWQAWDTELYDVNNYHYINNVKSIDIIDDNSVKISFAIASEKDIDLFTTISLDSKMIKIKTAVNNWYTGNKFLKVEFPVNVHNDYCSYETQFGITKRPTHYNTSWDIAKFEVCHHKFADYSEYSKGVSIINDCKYGFATHGNLMRLSLLRSPKKPDANADMGNHEMEYAILPHKGGLNMQTVQKAIAFNNKNTFCLPPDLASRFEKFVTLSPLHGNPPPSSTEANDNLNIVISSVKRGEDDFPESEYSLPHFGDLDSSKTLVVRVYEPLGGEICAQLNVSQEFKKVDMVDNLEEPLQKDITTLDSHSIAVHLRPFQIKIFKFYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN62</accession>
    <name>A0A1E5RN62_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">diphosphoinositol-polyphosphate diphosphatase</fullName>
            <ecNumber evidence="2">3.6.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g864</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>1,5-bis(diphospho)-1D-myo-inositol 2,3,4,6-tetrakisphosphate + H2O = 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:79699"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:74946"/>
            <dbReference type="ChEBI" id="CHEBI:77983"/>
            <dbReference type="EC" id="3.6.1.52"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:79700"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>3,5-bis(diphospho)-1D-myo-inositol 1,2,4,6-tetrakisphosphate + H2O = 3-diphospho-1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:56312"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:140372"/>
            <dbReference type="ChEBI" id="CHEBI:140374"/>
            <dbReference type="EC" id="3.6.1.52"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:56313"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate + H2O = 1D-myo-inositol hexakisphosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22384"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:58628"/>
            <dbReference type="EC" id="3.6.1.52"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:22385"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>6-diphospho-1D-myo-inositol pentakisphosphate + H2O = 1D-myo-inositol hexakisphosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:79703"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:230534"/>
            <dbReference type="EC" id="3.6.1.52"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:79704"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein-tyrosine phosphatase family. Atypical dual-specificity phosphatase Siw14-like subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.52" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN62"/>
    <dbReference type="FunCoup" id="A0A1E5RN62">
        <property type="interactions" value="135"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN62"/>
    <dbReference type="InParanoid" id="A0A1E5RN62"/>
    <dbReference type="OrthoDB" id="6375174at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052840">
        <property type="term" value="F:inositol diphosphate tetrakisphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14528">
        <property type="entry name" value="PFA-DSP_Siw14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000035">
        <property type="entry name" value="Tyrosine phosphatase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020428">
        <property type="entry name" value="PFA-DSPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004861">
        <property type="entry name" value="Siw14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF48">
        <property type="entry name" value="INOSITOL PHOSPHATASE SIW14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03162">
        <property type="entry name" value="Y_phosphatase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01911">
        <property type="entry name" value="PFDSPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="11">
        <location>
            <begin position="140"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="57"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ88331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="300" mass="35038" checksum="2F9C6A79B3123190" modified="2017-01-18" version="1">MQKEELSELLKEYEIGAIDINQLSEKLRTQLHLGKNGKALASLKDCPEFLPLANKILSNEDDSTDPLKYNTEGEEDEDQEGNGQEFDEDVDDKFDEEEQKWRSKHFLAHQEHTERDMTQDHRAPRTVDASFSQEMFPPENFSNVIGEIYRSSFPRRENLQFLKKQIKLKSVLVLLPEEYPQDLLQVMTDNDIQLFQCPMQGNKEPFIHIPDKTLTNALTIVLNPENHPILIHCNKGKHRTGSLVGCIRKLQNWSLTMIFDEYRKFAFPKARALDQQFIEMFNTKDIENVSMKNGWLPIEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNC3</accession>
    <name>A0A1E5RNC3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Vacuolar amino acid transporter 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g698</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNC3"/>
    <dbReference type="FunCoup" id="A0A1E5RNC3">
        <property type="interactions" value="563"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNC3"/>
    <dbReference type="InParanoid" id="A0A1E5RNC3"/>
    <dbReference type="OrthoDB" id="1684102at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005302">
        <property type="term" value="F:L-tyrosine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF666">
        <property type="entry name" value="VACUOLAR AMINO ACID TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="391"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="436"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="480"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="510"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="551"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="584"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="627"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="680"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="704"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="744"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="362"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="149"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="265"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="234"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ88386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="772" mass="86047" checksum="E654B5FDBC76FA10" modified="2017-01-18" version="1">MDIVKISSGNSNKIENDTVPISSSARSASMKIKHSNSQRHPKNSYYNGGAGSFTNPNNNSHSRRHSILLLSESVQSRKSGINMGTRNSRNSTSSTTLQNLASKNMAGSYGKSIDSKVFININSPNFKSENQDEILNSLSQHNYLNFQHRRKSNHSHAASHVSSSDVALSNSSVAVGDEDDEDVENRSESPSLNEHDPHLNQEGGDITRDIYSLAKNSKMKSKLRKNKSFDDLSSLHSTSRRGSTASALNVPGGFRRHHIVQKISETGTHANGNTPTESHSSATDSVHTFGEDGSPLPQPIESVPFLTRNFLEFLYVYGHFAGESFEDEFYPEDMKNGVVDGENTPLLMKHKRSIEKMSAKGTTSTFKAFLLMIKSFIGTGVLFLPKAFSNGGLFFCVFMLTFFGIYSFWCYYILIQSKVATKVSSFGDIGQKLYGPWMKVIILFSLVVTQLGFSAAYVVFTSTNLMAFVKNVFHLHEHLKIEWFLIFQIIVFIPLSFIRNVSKLSLPSLFANFFTMGGLLIVIFFVIKHLVIDLDMKPAEGIIYTFNPNSWSLFIGTAIFAFEGIGLIIPVQDSMKHPEHFPKVLLLVILTACVLFILVATLGYLAYGSSIDTVILLNLPQSNIFVNMIQFFYSMAILLSTPLQLFPAIAIIESKILKPRFVNHKLVTGKTDWKIKWVKNFIRSLIVIFTVGLAYFGSSNLDRFVSFVGCFACIPLVYMYPPMLHLLSCSKPKVAEKPTLKNKLLVTLDYGLLIFGGCSMLYTSYQCIFSPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RND4</accession>
    <name>A0A1E5RND4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Deoxycytidylate deaminase</fullName>
            <ecNumber evidence="7">3.5.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">dCMP deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g661</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.12" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RND4"/>
    <dbReference type="FunCoup" id="A0A1E5RND4">
        <property type="interactions" value="73"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RND4"/>
    <dbReference type="InParanoid" id="A0A1E5RND4"/>
    <dbReference type="OrthoDB" id="6710946at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004132">
        <property type="term" value="F:dCMP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009165">
        <property type="term" value="P:nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01286">
        <property type="entry name" value="deoxycytidylate_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000035">
        <property type="entry name" value="dCMP deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015517">
        <property type="entry name" value="dCMP_deaminase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035105">
        <property type="entry name" value="Deoxycytidylate_deaminase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11086:SF18">
        <property type="entry name" value="DEOXYCYTIDYLATE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11086">
        <property type="entry name" value="DEOXYCYTIDYLATE DEAMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="10">
        <location>
            <begin position="243"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00071582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ88397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="404" mass="46514" checksum="0E1D4F642D7E3DB4" modified="2017-01-18" version="1">MLLWCTTDYIGDEQARNNLLMDVLQQKFGLSKLSYIHDQKNQAHHTSHTESNGNTHEKEERVNSIRFASFTLLLDYVTVNYDKLFVIEDVYSLNDLKLLRKRPFFCHIHVDLPLNLRYELYKQNFAKPNHAEQSLPLIADFQQTLSNADSANNGKHNFENVQLDFPSFVNKDSYYHTTRKVLLLQHYDALLSCVPNFLTSPYLSTNAQDIAVVFSNFSMQVEKLLRNSKFFNCPKGAPPLRPSWDQYFMKLANLAASRSNCMKRKVGCCIVQDSRIIATGYNGTPRHLKNCFDGGCERCNSGNSKNLHTCLCLHAEENALLEAGRSRIAEKDCILYCDTCPCVTCSVKIVQTGITEVVYSQSYNMDEQSFKIFQEGHVKVRQYKHQDSDTLLYQVFENSYKECF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNH3</accession>
    <name>A0A1E5RNH3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein YIM1-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g926</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the YIM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNH3"/>
    <dbReference type="FunCoup" id="A0A1E5RNH3">
        <property type="interactions" value="159"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNH3"/>
    <dbReference type="InParanoid" id="A0A1E5RNH3"/>
    <dbReference type="OrthoDB" id="3509362at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050700">
        <property type="entry name" value="YIM1/Zinc_Alcohol_DH_Fams"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11695">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11695:SF294">
        <property type="entry name" value="RETICULON-4-INTERACTING PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13602">
        <property type="entry name" value="ADH_zinc_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="4">
        <location>
            <begin position="14"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ88435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="359" mass="39869" checksum="3137D72B683A8202" modified="2017-01-18" version="1">MTDIVKRRSIVFYTNAQKPELITTDLDLNSCYKPEEIVVKIHYAAFNPIDYILYKIASSWVAKKTFAKTFGRDFIGEVVKVGKNVPADQFSVGDKVAGLFQHLFGESGTMSDYLIFDPKAHKSIAKIDSGKVPISPENGAWPLVFGTSYQALTSFELKKDVDWAQKDKRVLIVGASTATGIMAVQIAKNELGAAVVDGICSAASFERNKEYGFDTLFDYKTGNVTQDLIDHVAKTGIKYDVIYDCCGNSYFFNCMDQVLKPKNENSYYVSIAGDSKINYANPAFPSIVGTVKKWFIRSYTYHQLLLVPNADVARVGIKMITEKKLSVPVDTVYSPEDFEKAFDKLISAKAKGKVVVSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNJ2</accession>
    <name>A0A1E5RNJ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g957</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNJ2"/>
    <dbReference type="FunCoup" id="A0A1E5RNJ2">
        <property type="interactions" value="1018"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNJ2"/>
    <dbReference type="InParanoid" id="A0A1E5RNJ2"/>
    <dbReference type="OrthoDB" id="272141at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14137">
        <property type="entry name" value="STKc_GSK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000688">
        <property type="entry name" value="RIM11p Protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050591">
        <property type="entry name" value="GSK-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039192">
        <property type="entry name" value="STKc_GSK3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057:SF0">
        <property type="entry name" value="PROTEIN KINASE SHAGGY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="119"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="15"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ88458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="457" mass="52453" checksum="36FDF374A5CE641A" modified="2017-01-18" version="1">MDISKITNNYTQQGSSSSSSNNSSNNNNNNNNNNSSNNNTNFSGTHLTPNANIHTNQILRVPSGEQMASQSRNFNPVAGQSSLSRTPLPGNLILVTKTVFQGHPTVQSPAIQQPVNITYPKTEVVGHGSFGVVFVTTIQETQEKVAIKKVLQDKRFKNRELEIMQKIQHINIVDLRYYYYEEDPQSDSLYLNLILEFMSQSLYQRIRHYVHLQTTMPHDEVQIYMYQLFKGLNYIHGTLGICHRDIKPQNILINPENFQLKICDFGSAKRLNPMEPNVSYICSRYYRAPELIFGSTNYTTKIDVWSSACVMCELILGQPIFPGESGIDQLVEIIKILGTPNKQDICSMNPNYMEHKFPHIKPIPLIKIFKREKDYLCIELISNMLKYDPFERFSALQCLCSSYFDKIRMNYNNEIVTNLKLLDFNNDTDFDYLSQDEKLIVKQKLLPHLYSSINPSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNK5</accession>
    <name>A0A1E5RNK5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ADP-ribosylation factor-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g960</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNK5"/>
    <dbReference type="STRING" id="56408.A0A1E5RNK5"/>
    <dbReference type="InParanoid" id="A0A1E5RNK5"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044612">
        <property type="entry name" value="ARL2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45697">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ88475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="212" mass="24159" checksum="FE9EF2D3FD50B530" modified="2017-01-18" version="1">MISSTIVRQLETLAEERPENALVCNAEKSLSHIQEVQPTVGFSIKNITYANKENYTGVLNIWDIGGQKSLRPFWNNYYDSSVEWFVWCVDASDTSRLAESVSEFRKNIEQCVVYNNNNNSHDVEGQIDKPAETAPLQGMLTNRVLVLLNKVDLTYDDSDSVDHRAQPEMTRLDFWFRNFCIVNLVENCVILPVSGKTGYNIVNSRLPDVLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNR5</accession>
    <name>A0A1E5RNR5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">holo-[acyl-carrier-protein] synthase</fullName>
            <ecNumber evidence="1">2.7.8.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g882</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.7" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNR5"/>
    <dbReference type="FunCoup" id="A0A1E5RNR5">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNR5"/>
    <dbReference type="InParanoid" id="A0A1E5RNR5"/>
    <dbReference type="OrthoDB" id="3972076at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="entry name" value="4'-phosphopantetheinyl transferase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050559">
        <property type="entry name" value="P-Pant_transferase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12215:SF10">
        <property type="entry name" value="L-AMINOADIPATE-SEMIALDEHYDE DEHYDROGENASE-PHOSPHOPANTETHEINYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12215">
        <property type="entry name" value="PHOSPHOPANTETHEINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="4'-phosphopantetheinyl transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="4'-phosphopantetheinyl transferase" evidence="3">
        <location>
            <begin position="128"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ88323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="268" mass="30604" checksum="0579659778ECE731" modified="2017-01-18" version="1">MVGKTEVIDQLYENHAVVVTVQAQKYINKLQNDTFFFEECLRLLPLNNAVLKKRYFEDQVTTLINRLLQLWGSRCAVGNDDLRNKIKFSKGQYGKPVLTDHHPQVTFSMTNGKLITSMFITRDIFQDVGIDIASVDDHTGDRFDIFHDILAPKEINSLQNPQFSAVEKRKLFARIWSIKESYTKFTGQGLNCDLQKIFLPDISPTETVIIRTSTVGTESNKMTFVSRWIDFGNVLTVCYPFGSTINSHALSFISVDLLDVIHDLQQSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNZ1</accession>
    <name>A0A1E5RNZ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ingression protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g752</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNZ1"/>
    <dbReference type="FunCoup" id="A0A1E5RNZ1">
        <property type="interactions" value="138"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNZ1"/>
    <dbReference type="InParanoid" id="A0A1E5RNZ1"/>
    <dbReference type="OrthoDB" id="270970at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052981">
        <property type="entry name" value="Ingression_C2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47052">
        <property type="entry name" value="CONSERVED SERINE PROLINE-RICH PROTEIN (AFU_ORTHOLOGUE AFUA_2G01790)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47052:SF3">
        <property type="entry name" value="INGRESSION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="16"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="410"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="410"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="453" mass="50933" checksum="379BF3904569B93F" modified="2017-01-18" version="1">MVEKNSIPGKQKLNLIKQTENMSQYENNFTGVYGTLIVFVHRAKDLINVRKMDKQNPFVILRIQHMTARSKICFRGGQTPSWSFLAKFEITPDIKPTLVLEVFHETEKAPKFIGKGTVDFTTAIFSDENDGDDRWIELTNGVEYAGKVYVEMSFKIKSASEINPKGKTVLDDIDQFDQSVASRQLPLLPHEIDPDNEFDDTSSVGGENTQFGYMHGVPGGFRQPVGKTGAQMSSGRIQLNRNNSANQNDLEENAYKSQSSMPTINSYNTGAPNFSNSYDSKHSKVPDSEPLFAKLKELKGKMFSFKNQPITDSESDMSRSSNSNNHNKVDFQALEKAVGVTGGADDTYFDEPSMSDEVDDGHEPRFKMFNMANSRRVTSRVTANMGGRYNQDAQHRPGMMNDGQRVESAYHTSFRQPSHLQSPTRNFTLYSNESSPERHSQKSPSRRPPPPSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP10</accession>
    <name>A0A1E5RP10_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g942</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP10"/>
    <dbReference type="FunCoup" id="A0A1E5RP10">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RP10"/>
    <dbReference type="OrthoDB" id="3972942at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="811" mass="92533" checksum="11B807BD56958FA5" modified="2017-01-18" version="1">MSATNTAPTSNAVSKTTAPNLVSVSLNENTAHQISAHTSNGQDQISPTANSQNDTTMGSGKEENNADINNTAPRGSISSYFPAFISSGINTTMQYLSKSTDLDEEYNKNMLLNGGIPFELLAPKFQSYIIKESQDLLKKEQESYFLVEKVNQNTRKLGVCNSKSFHCLIPIFDDSIIKLEADLSKLTDKTEDTITETRATPSLYVNNSLLLPGASISHMYHQKSLWSQIAMGVKDHYNLEPQRHLYLKKRTSSDSVYFAKSGSDSVKSLSKKIRIVSITCSLPEAYMQKTVACMPSSYELSTNLKKTLLENNPHCDIQTLSISLILQPVEDLLEVIQSIITRQKRFFKDVDAVFFAGYYHTVPLVIQTVESLIKKSFANPYNTEYPFKNAHIGLWGIESCLSSQTFFEHSSDVESNKKLYANCTKYEQDMLKNIHKFASSASLQSSIKWICQKTNVKITLTAKLYDNFMTLSQKLGVAYKHPNIMRNVWVDCNSFELCEPYISKDVENKELDNSTAHCCKLLVPKERCFEISLINNLILAINLGHHEQASFLLEKLAPFFISRSFNENTYPNVLKKKIDKDTREWLIKLEKDHPTWKNIVPTAENLHDFKRCECNSFTDFIEFLKYKSFAKQLVLKRAIQNDEKIYKAFIQNTLFTSSLVDSQKVVLFEKHELENIFDQHSQYEVVWSLHEFLSKFITFKTLPSDQGEKLYTNLCLSSGFLDSQEISGSLEDPARVEVGSKFSKSSSHEHELTKDIEYNRTTAESLLRVKNLYHQYWDWQPPVKGLKTLKKIFSVIEMYSTSEDFIRDIAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP37</accession>
    <name>A0A1E5RP37_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GTP-binding protein RHO4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g678</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP37"/>
    <dbReference type="FunCoup" id="A0A1E5RP37">
        <property type="interactions" value="127"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP37"/>
    <dbReference type="InParanoid" id="A0A1E5RP37"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000983">
        <property type="entry name" value="Rho family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ88636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="193" mass="21466" checksum="73FBC313A4E75353" modified="2017-01-18" version="1">MSPGNIIRKKLVIIGDGSCGKTSLLHVFTLGKFPNEYQPTVFENYVTDCRVDGIDIQLSLWDTAGQEEYERLRPLSYSKADVILIGFAVDDEESLMNTRIKWIQEVLRYCPGVPIILVGLKKDLEDNRGNPDFAHHGFIKRKDAEIIANEIGAKKLMMCSALTGEGVDDVFEAAVRTSLLVNKVQDNGCCVVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP54</accession>
    <name>A0A1E5RP54_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Factor arrest protein 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g733</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP54"/>
    <dbReference type="FunCoup" id="A0A1E5RP54">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP54"/>
    <dbReference type="InParanoid" id="A0A1E5RP54"/>
    <dbReference type="OrthoDB" id="18234at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040185">
        <property type="entry name" value="Far11/STRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021819">
        <property type="entry name" value="Far11/STRP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012486">
        <property type="entry name" value="Far11/STRP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13239:SF4">
        <property type="entry name" value="AT25231P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13239">
        <property type="entry name" value="PROTEIN REQUIRED FOR HYPHAL ANASTOMOSIS HAM-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11882">
        <property type="entry name" value="DUF3402"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07923">
        <property type="entry name" value="N1221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01293">
        <property type="entry name" value="DUF3402"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01292">
        <property type="entry name" value="N1221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Far11/STRP N-terminal" evidence="2">
        <location>
            <begin position="291"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="Far11/STRP C-terminal" evidence="3">
        <location>
            <begin position="674"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="514"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="590"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="181"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="623"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ88656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1049" mass="120546" checksum="78602F0DE90ADB96" modified="2017-01-18" version="1">MPLQEYSKEDYSKSSGPLSKKSIDKDLVQESGGLLSTPSKSGKVPNTPLSNNSQSETGAGPQRPKLPSRSSSGSGTLNQRLAKLSSPKTSSGFWASQTVSPPLAPIQGLDQSEFTLNDEEHFDVGSFHKNSSQMHRLNFMDQPSLASHDTNGALMKDEDGTSSSDEEDDETFANVDEMDGPISPSNSSDEISSLFLKKEKSNSVPSSPVDEKSHFFDKHIQKKDFTLKQFAESKDQVDLGPVKLLETDFNLPVDHDLQKQLERRAFEIIESAKNNKEKSSNYECVYQPITKPVLHWEHKGFYYFEHEISLWFHSFGNLSIMEELKNCESQVVYAQPFPEYQFNDIAKLSQICYFAIGFFHHCTNLGTLEKTVKLNNWKLLKHMSKEILASFKQHAILCRDEKTHLAEYAKLLKYSATTLYFMIVVAIDERKKENKSQQQIIDSFCDEIDNLELLEFLLEYVEQWRWSCKLSMHIRSILLILNKLLVLQFGNETTYEKIHDEICQSHDIIDQKAHSKQGERLTKTHTGTSGNHNLADHAKHSLKTTPSQFEAFREDLTSRYPTAVMKPVLDYKYGESTSLTQFIDIPRPKKKYTSNTGLPEPQHDIQLSGRNDTPFPSPSSSPKTNTESFQRNDGSFKKPNGKKSYQTNLNYPFMFPTFSESEKNISNVSETGLPYNVQEAMDIMQKNIHFTLQDHQLANEKLLFMQKERGCLESQKIEGSTQIKNFDQYNLPTPNNNVIMARIEKLYQKTLPNFNSLIAVIMQLMELNLNDKKYETCSSDFQKRGFMSKQVTLSAGMSILFLLAKWFKLSHVCKFQYFASLLYDAKFIEMSTKLMEISFTVDRYNSIFKKKTDCTFWHECERLYEEGITKSDADLFGSYSEEDFEFELGKTMSHSSSGSENSHQYDDEEDVNLVYLDIEYCTFSLLQQVLGSKTQRLKFLPLNIGITFKKLYQIFNLQLYDPLLSITKELTPFKSKKWKSEHMDLISGVYLYQDLSLVDNWVTGKDTSAEINDAYSEEFALRTLLQFYNFRKYPDTMKYLGYSEKPIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP56</accession>
    <name>A0A1E5RP56_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ribosome-associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g784</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP56"/>
    <dbReference type="FunCoup" id="A0A1E5RP56">
        <property type="interactions" value="107"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP56"/>
    <dbReference type="InParanoid" id="A0A1E5RP56"/>
    <dbReference type="OrthoDB" id="10031169at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF299">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="2">
        <location>
            <begin position="12"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="1">
        <location>
            <begin position="239"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="911" mass="104463" checksum="FE9D66D23800FFE1" modified="2017-01-18" version="1">MSLIDTQNVKIDKYQLFLDINPVKTNFNGSATIFFKTPLNGQELGSIKLYVDDLILIDYKINNSDSYNDSELDITNHTTTLPLHDHCEISKITINYIGKISTISTFKDKTYGIFKTNIMNESGNANNIVISTHCQPNFASKIFPCLLNSKSKIQLTIKTLSKFKVVSNTGVLQSQLLESSDERMVTFKETPYIQHAVFGFTLGDLDHIQTTVTATTSSGESKKIPITVYSPISIQDCSFALDIIAKYFAKLNNEVFKKNPYPLDKLDFVALPFLSDYAMENHGMVSIQMNHLVLPKLVKNSAEYQQMEQLLCHELVHLWMGNYISFHWEHLWFNEAFATYMANHIIPNSFQSDDYLTNYHATCLNFTTQNTNQKTIANGIPTHITFNSTTDQLFNPQNYVKGIPVIRALVEMCGFDKFIQSLSQFFTNYEEVIDPKEIFEKNGIADSYDWFVSQWKECPVISVSKESSTNVENSPVYKIKGISENDAFPVFLNGGVTLKAKDLMLESNRAVLGKTFLFNEQGHGLYRVKYEGHGAIQNLLNNLPKIAEFDFYAILQDLQHFINTGTNINKYDISLPIEILTRMVSSQEIFVTLKNHDNNKNYTRAICMILEILEKIQLSVVKYGSGNATEPILGVDIYNNVLKPLFEIVKPQLFDTEMMEFLLEDVSEQEWSILSHLIFLLKTDEQVYEFSGAVIKSILSGRPIKSKIPMEIVGSCMASYSYGVDNVKDWKKIFNMVKNCEPYMHHIDYNSMDFQSVKEASFYMQNAITQNIGFVISPSIVSKLLNFIYTNIESTSIELLLFGVVFNQKQPDLKKLVWEWYQTNFDNIAQRSLRKGSKDAAEMLKTLQNISLLVFSMFLIEDSSKIDQFIILKHSKFNQDLQLDKIWALVKEREMHYIMLYQLLLQVKYDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RTX0</accession>
    <name>A0A1E5RTX0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g573</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000004">
        <property type="protein sequence ID" value="OEJ90248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RTX0"/>
    <dbReference type="InParanoid" id="A0A1E5RTX0"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="92" mass="10618" checksum="066663754C5ABFE3" modified="2017-01-18" version="1">MNSWSFNDKGTKIDSQRLEDTGIAMRGIVAMVMQVRRDRRSRRRDMIDGMGSGVSMDTPGLNEQANTDAMTVDWENDVLVLIRRWWAVTIER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RUZ8</accession>
    <name>A0A1E5RUZ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GTP:AMP phosphotransferase, mitochondrial</fullName>
            <ecNumber evidence="7">2.7.4.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Adenylate kinase 3</fullName>
            <shortName evidence="7">AK 3</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ADK2</name>
        <name evidence="10" type="ORF">AWRI3579_g413</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a ribonucleoside 5'-triphosphate + AMP = a ribonucleoside 5'-diphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:13749"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon GTP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent GTP hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adenylate kinase family. AK3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.10" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RUZ8"/>
    <dbReference type="FunCoup" id="A0A1E5RUZ8">
        <property type="interactions" value="339"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RUZ8"/>
    <dbReference type="InParanoid" id="A0A1E5RUZ8"/>
    <dbReference type="OrthoDB" id="439792at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046899">
        <property type="term" value="F:nucleoside triphosphate adenylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006172">
        <property type="term" value="P:ADP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046033">
        <property type="term" value="P:AMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046039">
        <property type="term" value="P:GTP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046041">
        <property type="term" value="P:ITP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000106">
        <property type="entry name" value="Adenylate kinase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03169">
        <property type="entry name" value="Adenylate_kinase_AK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006259">
        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007862">
        <property type="entry name" value="Adenylate_kinase_lid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028586">
        <property type="entry name" value="AK3/Ak4_mitochondrial"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01351">
        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05191">
        <property type="entry name" value="ADK_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Adenylate kinase active site lid" evidence="9">
        <location>
            <begin position="139"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="NMPbind" evidence="7">
        <location>
            <begin position="37"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="7">
        <location>
            <begin position="138"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="16"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="64"/>
            <end position="66"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="95"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="148"/>
            <end position="149"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ90767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="227" mass="25095" checksum="22F51CF6C8CF37B0" modified="2017-01-18" version="1">MSALKPLRLLLLGAPGSGKGTVTSRLLKVYPGIQSISSGDLLRRQIAQKTHIGLEALNYTSKGALVPDSVITKLIVDDLKENIINPQVQHWLLDGFPRKVSQAEDLDAALKPAGEKSVFNLVVEIDVPQSVIVERITNRRVHVPSGRVYNLQYKPPKVPGKDDITGEALVQRLDDTEEIVTKRLNDYNALVSPLKKYYRDLGILKTVSGETSDIVFPKLLDVIQNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVL0</accession>
    <name>A0A1E5RVL0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hsp90 chaperone protein kinase-targeting subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g293</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDC37 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVL0"/>
    <dbReference type="FunCoup" id="A0A1E5RVL0">
        <property type="interactions" value="458"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVL0"/>
    <dbReference type="InParanoid" id="A0A1E5RVL0"/>
    <dbReference type="OrthoDB" id="440202at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.610">
        <property type="entry name" value="Cdc37, Hsp90 binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004918">
        <property type="entry name" value="Cdc37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013873">
        <property type="entry name" value="Cdc37_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013874">
        <property type="entry name" value="Cdc37_Hsp90-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038189">
        <property type="entry name" value="Cdc37_Hsp90-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013855">
        <property type="entry name" value="Cdc37_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12800">
        <property type="entry name" value="CDC37-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12800:SF4">
        <property type="entry name" value="HSP90 CO-CHAPERONE CDC37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08564">
        <property type="entry name" value="CDC37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08565">
        <property type="entry name" value="CDC37_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03234">
        <property type="entry name" value="CDC37_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01069">
        <property type="entry name" value="CDC37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01070">
        <property type="entry name" value="CDC37_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01071">
        <property type="entry name" value="CDC37_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101391">
        <property type="entry name" value="Hsp90 co-chaperone CDC37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cdc37 N-terminal" evidence="9">
        <location>
            <begin position="2"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc37 Hsp90 binding" evidence="8">
        <location>
            <begin position="181"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc37 C-terminal" evidence="7">
        <location>
            <begin position="400"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="482"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ90889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="503" mass="57655" checksum="332C9BD30B61795F" modified="2017-01-18" version="1">MPIDYSKWDKLELSDDSDVEVHPNVDKASFVRWKQQSIHEERAKRNQDIKTLEAQMTMYENLNKRTDNMLKNMPLSEFGSLENIVKYLNSNFDKSERSEGEYVDKDIPTYNEMVEDLIEQLQKELKEANKDPTDGSLIVHKLAEHRLKIQTVMNEGKIKLAKLYEEKANHISADDMHDAWNSSFVNKSGKTVAPSVGAPDVAGTTTSSSVSAANFQNPPISQTLPSSVKSFISFDAEEDILKLDPKTKAFGEIPASDFKKSQQFLLENMEILSDQQKDALIMSAFEYEMEGDTKKCYQVIHQGELMQFIKELYDLKKIPYLHTSEMENIINNFFERIFFNTQNEMGKKQFLQSVQTKYDHIRTRSKILQEQDARAIGEDESEGVETIQLKSLDDTVQLKINLPDFESTDPNEQLKVSKFNELSPEMQNAVKTESLDVINDVLSRMSVSEGEKVLEIFDQADIIGINAYFDNEKDFEELQETYQEQMTQADQGELSAADQDDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVZ2</accession>
    <name>A0A1E5RVZ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-(apurinic or apyrimidinic site) endonuclease 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g484</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="11">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVZ2">
        <property type="interactions" value="491"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVZ2"/>
    <dbReference type="InParanoid" id="A0A1E5RVZ2"/>
    <dbReference type="OrthoDB" id="391817at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020848">
        <property type="entry name" value="AP_endonuclease_F1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF4">
        <property type="entry name" value="DNA-(APURINIC OR APYRIMIDINIC SITE) ENDONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06839">
        <property type="entry name" value="Zn_ribbon_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00728">
        <property type="entry name" value="AP_NUCLEASE_F1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GRF-type" evidence="15">
        <location>
            <begin position="551"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="462"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="464"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="488"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="10">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="359"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="12">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="12">
        <location>
            <position position="335"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA substrate" evidence="12">
        <location>
            <position position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ90965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="596" mass="67182" checksum="E1990A3ED5DD156E" modified="2017-01-18" version="1">MSDAKFIIPEKKPEDQIRFLSFNVNGIKTFFQYHPFNKTGNSLDKVFEAFEADVVTFQELKTSRDTIQKYGKLNNYHSFITLPRKKLGYSGVGCWVRKITDPQDPLYQGMQVLKAEEGITGYLDAPKSSISKSGPLSSSAFSNQKCYRDDHAQSIGGYQDMDIIWGNDYERALELDSQGRCIIIELSCNLVVFSCYCPANSMGTDDGELYRMQFMKALFKRARNLYQMGKKVXIMGDINISKDLVDNAESMESDSENGWKGVPSTQEMLHFIQNTRPSRLLMNQQLGDSTDKDCAKTGFLFDSTRKIQGYNKPQMFTVWNTLLNTRPMNYGSRVDYILVSEADSIRNADILPEVQGSDHCPIYTDLQIKXSGKLNTVPGIPKFECKFHYKLISHNILDMFSKRKISDAFSETQIQKAPHSKTDHXTALNGPVKKKAKIDSFFKPVSRPVTPKTIKIGSSKGLFVEDDDDDDDDDXEEEEENVDVVQRKSLQPSASSRIQLLKSKNTDNGKKSLNGGDTQVAVVKKRASKFTIEQSELGKKIIAAFGNVPKCKHGINAVLRTSKTQNNFGKKFWVCGKPRGADLEDPNTSCGFFQWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZD4</accession>
    <name>A0A1E5RZD4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CTD kinase subunit gamma Ctk3 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000002">
        <property type="protein sequence ID" value="OEJ92159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RZD4"/>
    <dbReference type="OrthoDB" id="21266at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024637">
        <property type="entry name" value="Ctk3_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12350">
        <property type="entry name" value="CTK3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTD kinase subunit gamma Ctk3 C-terminal" evidence="1">
        <location>
            <begin position="392"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="475" mass="54601" checksum="44E3FF0E47B89CE8" modified="2017-01-18" version="1">MDSANGKGTVSATASGNIKNKYXLLIKKLHKSDLVFQNLNYSNSLKDKEDXNTKKKIEGEPKTVNNQKALQSSHVNDENVELALKNDTIELQNLCKNAIKTEILNFYLTYYETYHADFHKILMDYILKMNKLDRVYFLIYYGHLILQLNMKSMIDLQGKGNTAYEVLHNYLLKDLSLLYELVLYKNGISSSASGLLDESKSIALNESNANWASSVTNMPIALQMFANICKIFNQKNGKDETCRRIVLQFDDKRLNTFNPQEEEIVLAKKQFSEFFTITSDSSETHSQNQSYYEKLFPQFVANENDVMLKSDQRALIETWKVLEQTYHLKQFSIYYFACWGFPGNKETINEESGKVTTNNETKTTFSDDVAHSTFSTAQGTANNNKNGVQTDESLLHRIEFDRERHKKSKEQNWTVERRAGVLEYEEFQNAWNAIGNPNQSSLNNADSNNHDXXYSLSLDDKQCIQALQTIYLQSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZE2</accession>
    <name>A0A1E5RZE2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Heat shock protein SSC1, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g194</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZE2"/>
    <dbReference type="FunCoup" id="A0A1E5RZE2">
        <property type="interactions" value="1708"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZE2"/>
    <dbReference type="InParanoid" id="A0A1E5RZE2"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11733">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000014">
        <property type="entry name" value="Chaperone protein DnaK HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000003">
        <property type="entry name" value="Heat shock protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000007">
        <property type="entry name" value="Heat shock protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000004">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000003">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00332">
        <property type="entry name" value="DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012725">
        <property type="entry name" value="Chaperone_DnaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02350">
        <property type="entry name" value="prok_dnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0346">Stress response</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="598"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="622"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="555"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="603"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ92370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="646" mass="69785" checksum="679945C40BDFA531" modified="2017-01-18" version="1">MLSATRNNSAIKSLVAKRLQSSKVQGAVIGIDLGTTNSAVAIMEGKIPKIIENAEGSRTTPSVVAFTKEGERLVGIPAKRQAVVNPENTLFATKRLIGRRFEDAEVQRDIKQVPYKIVKHSNGDAWVEARGEKYSPAQVGGFVLNKMKETAESYLGKSVKNAVVTVPAYFNDSQRQATKDAGQIVGLNVLRVVNEPTAAALAYGLEKAESKVIAVFDLGGGTFDISILDIDNGVFEVKSTNGDTHLGGEDFDITLLREIVKKFKEESGIDLGNDRMAVQRIREAAEKAKIELSSTMQTEINLPFITADASGPKHINMKFSRAQYETLVDPLIKKTVDPCKKALKDANMATSDIGEVILVGGMSRMPKVVDTVKQIFGREASKSVNPDEAVAMGAAIQGAVLAGEVTDVLLLDVTPLSLGIETLGGVFTRLIPRNTTIPTKKSQIFSTAAAGQTSVEIRVFQGERELVRDNKLIGNFTLSGIPPAPKGVPQIEVTFDIDADGIINVSARDKASNKDASITVAGSSGLSESEIEQMVTDAEKFKEQDQARKESIESANKADQLCNDTEASLKEFEAKVDKLEAQKVKDLVAALRETIAKVQSGAEDVKPEELKAKTEELSASSMKLFEQMYKNQQQDGSAPKDEPKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZW4</accession>
    <name>A0A1E5RZW4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 19">3-isopropylmalate dehydrogenase</fullName>
            <ecNumber evidence="7 19">1.1.1.85</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">AWRI3579_g168</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17 19">Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:32271"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.85"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="19">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="19">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 19">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 19">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.85" evidence="7 19"/>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZW4"/>
    <dbReference type="FunCoup" id="A0A1E5RZW4">
        <property type="interactions" value="937"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZW4"/>
    <dbReference type="InParanoid" id="A0A1E5RZW4"/>
    <dbReference type="OrthoDB" id="419183at2759"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00072"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003862">
        <property type="term" value="F:3-isopropylmalate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000006">
        <property type="entry name" value="3-isopropylmalate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004429">
        <property type="entry name" value="Isopropylmalate_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00169">
        <property type="entry name" value="leuB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42979">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42979:SF1">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="16 19" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9 19" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="11 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 19" id="KW-0520">NAD</keyword>
    <keyword evidence="13 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="20">
        <location>
            <begin position="6"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00019276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU004445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OEJ92424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="366" mass="39206" checksum="521EDFAC72FCF964" modified="2017-01-18" version="1">MSEVKKIVVLPGDHVGQEIVNEAVKVLKAIESCTKSKDVKFEFQNHLIGGAAIDATGVPLPDEALEASKNADAVLLGAVGGPKWGTGAVRPEQGLLKIRKELGLYANLRPCNFASDSLLDLSPLKPEIAKGTDFIVCRELVGGIYFGERKEDEGDGVAWDSEKYTVPEVQRITRMAAFLALQHNPPLPVWSLDKANVLASSRLWRKTVEETMKTEFPQLTLQHQLIDSAAMIMVKSPTTLNGVIICNNMFGDIISDESSVIPGSLGLLPSASLASLPNTNKAFGLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLDMVDEGIAVEKAVKQVLDAGIRTGDLRGSNSTQEVGDAVAKAVCELLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZZ5</accession>
    <name>A0A1E5RZZ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g96</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZZ5">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZZ5"/>
    <dbReference type="InParanoid" id="A0A1E5RZZ5"/>
    <dbReference type="OrthoDB" id="407558at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000029">
        <property type="entry name" value="peptidyl-prolyl cis-trans isomerase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0697">Rotamase</keyword>
    <keyword evidence="4 7" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="8">
        <location>
            <begin position="5"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="337"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ92379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="400" mass="45346" checksum="5C81865662474EC7" modified="2017-01-18" version="1">MTCAYLDIEINQNKIGRIVVELFQDKAPKACENFLHLCKGDVSLPRQASESSQEQDEQVLSFXNNKFHRVIKNFMIQAGDIVYGSNDGEVSKELLGKGGCSIFAQSFPEQNSDISISGNFEDENLGPLDEPMTLAMANMGQPNTNSSQFFIVTYPSPHLSNKHSIFGKVLHGKSVVRTIERTPVDSSDGVPIKNVVISGCGIWDKSMPVPLYVSCASTIGGDNYEEYPDDEKSIAEEDFSKAFAVTNTIKESGTFLFKEKKYQDALFKYLKSLRYLNEYIPDYDVDQDNNKKFCQLKVKLYLNICLCYFNLRNYDESIKFATYVLELDYKVDNVDEGKAYFRRASCNLQKKKLDRALGDFKKCREVNPNEKAIDTKIAQVEQQIAENKEKTKKSIAKFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S095</accession>
    <name>A0A1E5S095_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g179</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5S095">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S095"/>
    <dbReference type="InParanoid" id="A0A1E5S095"/>
    <dbReference type="OrthoDB" id="2354757at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035838">
        <property type="term" value="C:growing cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051380">
        <property type="entry name" value="pH-response_reg_palI/RIM9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009571">
        <property type="entry name" value="SUR7/Rim9-like_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28013:SF8">
        <property type="entry name" value="AEL027WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28013">
        <property type="entry name" value="PROTEIN DCV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06687">
        <property type="entry name" value="SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="96"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="332"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="439"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="508"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="281"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="359"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ92506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="624" mass="69977" checksum="72155C37B147555C" modified="2017-01-18" version="1">MSYTKAFSISTVISILQFTSLAALIIACVTAPVFHQISLSSYDGYRYGVFGYCHGGSDTNECSKASANYQAYSLQNSENENTWKMASTARKTLSELLLVLPVSAGVVFFSMVFNITYGFISSGSKXLFLIDLLLTFLAFSGSAFSCIVVFLLFYPHVNWCAWILIPSAVLNLVSLPLTFLVYKNKRDASLDSRDSDNEDTESTDDYSSRLDRGFVKTNLLNREDTAVENEKNDISITNLNYKDDDFFAQGIPKAYQSNNNSSTVLGSNLALKTTAHTNNSGSFDFTNNNNNNNNNNINDENDENDFDMSLDDVNRQQPFSAVANKRFSSNSNVYPSVEQAPTSANSGKTFPQSVRDPSDSSSKYSEMPLNNQNSYPSLRQPNKFGMQNXPRRTDTLNTADLKPGSVLQLKDDAPPTLSNYMKQNDAIDEEDEEFIRKNTIDPAERPDIDDDGISDPGSDFTSVSQRGINPEYFKKQQMQQVRPGPLSAQQPQVPVPQNRQRFNNQTMPQQQNRLAYPQQPQQPPMQGAYRQPYYNSAQQYQQNYAPGYAGQPQMMRYNNMNTPTNYAMQNSQAFPNASMGPANRFPQQQQPMAYRPGYKKKLPGNNMPGFTPASMQKGNIYNFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S0C8</accession>
    <name>A0A1E5S0C8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DNA polymerase epsilon subunit C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g49</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5S0C8"/>
    <dbReference type="STRING" id="56408.A0A1E5S0C8"/>
    <dbReference type="InParanoid" id="A0A1E5S0C8"/>
    <dbReference type="OrthoDB" id="636685at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008623">
        <property type="term" value="C:CHRAC"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050568">
        <property type="entry name" value="Transcr_DNA_Rep_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252:SF54">
        <property type="entry name" value="CHROMATIN ACCESSIBILITY COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252">
        <property type="entry name" value="HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="233"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="238"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="278"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ92465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="322" mass="35459" checksum="59EAEAC9209BCB73" modified="2017-01-18" version="1">MSFAIDNEHSSVSAGQESLHTNVSAEQESLHTNVSAEQESLQQSLSNIDNVLLLPLGKIKKVCKSDPDYFLQSNLASVVGGLATQLFIEKFTEDALLQAHMRDKRPVPSVVSPEENGSAQNKPNNVNVIKTRLTYEDLHQTVSKDDKYMFLKDIIPKQKLLHSLVRENKIRYSVLESGQQMLNFQKMNANEELMDPSRAVLGGAGDVMEGIEEANGETVQEQAQPLAQPLAQPLAQDQTKDHAGEDVHTDEEDVDGLEDRSGDGQNGSHLVQEIDMDGTTKEDRMIDNENETGGEQVADGQNSKKGVELIDFGIDSTTDEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0G0</accession>
    <name>A0A1E5R0G0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein GVP36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4577</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0G0">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0G0"/>
    <dbReference type="InParanoid" id="A0A1E5R0G0"/>
    <dbReference type="OrthoDB" id="5549748at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018859">
        <property type="entry name" value="BAR_dom-cont"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10455">
        <property type="entry name" value="BAR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="413" mass="46530" checksum="A37235D86952A4DE" modified="2017-01-18" version="1">MSLGEAEIAVTEEPAPIAAAKKSAQIAETKEATEDSEAXIPSDXTKSEEAQPQAKTETDTEKPKPASNQGFYSFFNKVDTFLSSTASQIQSSLPEETSYLNKAVKKNLFVLNQKLNDFTQSNSNFEKSKLPKTYLELEQKCDCIEKMLKKLLVVTETYXIEGYDYPPNLSEGFSELLTFGKAEKSESSMEQSTEKHIPEDTDSGKKSHFLPRSFSQALSNAFKESHSVVLSIARKNSVGHVDVDEEDESVQDLIKVFDALKDAHYEMDEVKANMDKLMSDIFNQGLKDLISKDLRKIHNSRKQVESARWDLDALRYELLDKTITEQDPEFKEKEKLEDAFVTKTTDAVSIMGEFIDSVDLLKLLKLFNEFQLDYHKKCAASLQTSLDVLNSLNLDEDHDEETEDENGVEATDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0P4</accession>
    <name>A0A1E5R0P4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein SNA3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4394</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0057 (PMP3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0P4"/>
    <dbReference type="FunCoup" id="A0A1E5R0P4">
        <property type="interactions" value="91"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0P4"/>
    <dbReference type="InParanoid" id="A0A1E5R0P4"/>
    <dbReference type="OrthoDB" id="2802411at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000612">
        <property type="entry name" value="PMP3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01679">
        <property type="entry name" value="Pmp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="75"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="128" mass="14600" checksum="C9416DC3A583D16E" modified="2017-01-18" version="1">MSTYSINKDDLILCLVALIFPTIPVIMRKGFWTKETLLNFLLLILFAFPAALHAWYVIYKTSNLRSDEEYDRLQGSLESGFASDEEDEQPPQDQHNNVAPPAYANVTTNDNTDNKNHNGFPLDNKVQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R154</accession>
    <name>A0A1E5R154_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">t-SNARE affecting a late Golgi compartment protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g4058</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R154">
        <property type="interactions" value="272"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R154"/>
    <dbReference type="InParanoid" id="A0A1E5R154"/>
    <dbReference type="OrthoDB" id="546861at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd15851">
        <property type="entry name" value="SNARE_Syntaxin6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="3">
        <location>
            <begin position="145"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="237" mass="27235" checksum="0AA513F7A4BCB9B8" modified="2017-01-18" version="1">MSNEDPFYDVLNDVKDQLKDLNVKKNNKNNNNNNNNNNALSSASPEVKEVYETVQDLKTSLQLSKPYLEPTELQKRESEISGLENQYYVLLHNLNNTTSTNNISDSIELQDHHATFANAGVXTEDFNIPENEDMDAQDKFTHLESQMLHEQDDQLDQITKTMQNLKTQANLMGSELQDHTELLDSIDTNMSTLQGKLKKSSQKLQWIYEKNKERYNTICIMLLIVVLIILLFLTIIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1E0</accession>
    <name>A0A1E5R1E0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal lysine N-methyltransferase 4</fullName>
            <ecNumber evidence="1">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4090</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that monomethylates 60S ribosomal protein L42.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SETD6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1E0">
        <property type="interactions" value="295"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1E0"/>
    <dbReference type="InParanoid" id="A0A1E5R1E0"/>
    <dbReference type="OrthoDB" id="341421at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1410.10:FF:000007">
        <property type="entry name" value="Ribosomal lysine N-methyltransferase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011383">
        <property type="entry name" value="N-lys_methylase_SETD6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF34">
        <property type="entry name" value="N-LYSINE METHYLTRANSFERASE SETD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011771">
        <property type="entry name" value="RMS1_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="2">
        <location>
            <begin position="25"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR011771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="487" mass="55608" checksum="B489C60E183DDAD9" modified="2017-01-18" version="1">MDSFEQXTXNFTDWTATAAAINISDGVVIKDFRQQNQGRAVIAEKDIKKGDXLFKIPRSTLLNVTTGAIAKDKTVAEALLHKIGHWEGLILCIFYELKMKKXSSFWYAYFNVLPDMQSSKPFDNLLYWNDEELQXLQPSLVLSRVGVEQSREMFENCVAYAKDFGFEFTFDWKEFLYVASLIMSYSFDTERADYNGDSEDEDLEILDESQTVDVYKDGYYKSMVPLADMLNADTHLCNANLTYSETHLVMVATRDIKAGEQIFNTYGDHPNSELLRRYGYVEWAGSKYDFGEITMANIFVVLAEVFGKKIDDVAALLEAISDDIDLLENVLEGEPIVIESYDCYNEGDISLEGVCLIQVLCLMLEFELTTSSPNFTKIVKKCIKQVSKSKITQRCQQVLKKVLEVRISEYPENGNQEVLTKPGSNYILSMAQRVLNSEVLSIKECLNKVDQNYKCVEAEEYIPNIDFVGKKRPSKEAAPRFSKKQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1E2</accession>
    <name>A0A1E5R1E2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zinc-regulated protein 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4329</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1E2"/>
    <dbReference type="FunCoup" id="A0A1E5R1E2">
        <property type="interactions" value="58"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R1E2"/>
    <dbReference type="OrthoDB" id="3973129at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="440"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="502"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="578"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="625"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="863"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="322"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="449"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="520"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="582"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="598"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="871"/>
            <end position="887"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="944" mass="106025" checksum="7D4FA3C5CEDCF6D0" modified="2017-01-18" version="1">MRSFIKAKRHSAPSENDISSPKSDIEQAGHERHSFSSIRSSNSSQPSGSHSSQVDCKISPTIESATITKHAKTNFFQRLTKKSSTTFKSTSPQVNAESNQAGTVHIQKSALASLGASTGSSLFTLPHASHNSDSASSPRHSQDSDSISNSKKPLNLQTAYRYQRKSKTKYSSSSLSSPDDYLPAIQGTIAHEWGSPRVIRPGSPIILDKKSSIAKAEKKVVFESIERQDDYRKSDSRRKSNRLARIFSNQDFLNLSLENEEDKHLIDKLKAVSSTENTTDDTERYGFSSASTPQHMNLDAISWPAEDRETENHVMDKENSFRRSSTTSSNESSDGSKFSFEFNSSNGRKASVKYYSKEGNEDSGVYFDELFEDEDLDEDMNVDECDDFGDFDEHNDYSMIYGAGTHAEEEEESPGMNNKASSFRFNDITALTDDEVDSLHNTSKTCSFQEEHANNTNDYHDSSESDDRDSVKIDSTFFQEPPLSMQAKKEKAITGFNDLYQLSDEEDEVQDEKLDKDINNYDEESYSTDDFDDNNQESDMNGLEANGGDTKYTENVDFVKNSTSKMVKPQTVKKFNDLFALSDDEEEEEEETGKAEAEFDELNDDADDDYASFYDFEIESLRSYSNSSDFTPRKPSRSKNSENELHNFSTPVLISTPASPLGKNLTFSPLFGKSKLEKSSNDPLGLAALALSPKTPRSPVNRLPPNSILKYHDLNSNLDSGFVPRTMGDLFFIDEVEEEHNVNEDDLYLDEINKVPEDFDFESNADSVTQNATGSNLYPMQTNNNYLRKMSDFSRRKSYKGSHSFFEKPTSMIKSNKPISSKVELKKQNKTVTFFHINNQQLQRSSSDPGIIVVKKPNLARHQSETLLDSADIKKKNDKHEKTDKTDLPGTDLAKSTTESFEKDSYSMFQTPSKFSEANSLSPIQEGLSSVETSPRVFLKKPFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1T1</accession>
    <name>A0A1E5R1T1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Anaphase-promoting complex subunit DOC1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4367</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the APC10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1T1"/>
    <dbReference type="FunCoup" id="A0A1E5R1T1">
        <property type="interactions" value="719"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1T1"/>
    <dbReference type="InParanoid" id="A0A1E5R1T1"/>
    <dbReference type="OrthoDB" id="3972478at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08366">
        <property type="entry name" value="APC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016901">
        <property type="entry name" value="APC10/Doc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004939">
        <property type="entry name" value="APC_su10/DOC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12936">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12936:SF0">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03256">
        <property type="entry name" value="ANAPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01337">
        <property type="entry name" value="APC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51284">
        <property type="entry name" value="DOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="DOC" evidence="6">
        <location>
            <begin position="85"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="291" mass="33088" checksum="201296FD50A85C96" modified="2017-01-18" version="1">MDYEQYLQLVDAKVPDIMSLYEYELKRLKENTTSNVRYLDEDKPNVAHDYVWSMDMYSELSQDAQLTTGDDPNEYIVTEGGKALSAEAAYERYMIGIKKIQNESSCLDVTNMVYWKASSSKADRPIDNIADSNPATFWQSDGFLPHIIEAHSAKKLSLISIDMFFSLTMDESYSPYLIKVYAGNSLLDLTFIRTMEVLNVNGWVSFRFSSNSGNSNTLECQHLKFIIYTNHQNGKDTHLRGIRLLAKKNTNNGLEDKALSFTGEQSRFLSQSNGVGSSTDNLPFDEHLLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1W6</accession>
    <name>A0A1E5R1W6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Serine/threonine-protein kinase KSP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4101</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1W6">
        <property type="interactions" value="563"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1W6"/>
    <dbReference type="InParanoid" id="A0A1E5R1W6"/>
    <dbReference type="OrthoDB" id="3971529at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF30">
        <property type="entry name" value="MATERNAL EMBRYONIC LEUCINE ZIPPER KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="45"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="419"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="522"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="600"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="717"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="919"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="420"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="538"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="602"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="620"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="735"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="761"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="924"/>
            <end position="936"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ80884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="985" mass="110842" checksum="42B1C9527E0D1F7B" modified="2017-01-18" version="1">MGVSTMVGSTMPPLSTPPLNTPTHTPSPIYDYDVYKERGVINNRYQKIEDISKGSYGYVSLATDLKEQNLVAIKYIFKLENEMDEDCSNESNKSKNGNDCNRYSNVDPLVRSKLSKNICFEAIHEVDILTKIGNEGSLHNIIKLVDYFDSYIVMEYCMGGDLYDNIKSDIVPKSTKMITHLGLQMINAVEFAHKQKVYHRDIKPENILISNVKNWTIKLTDWGLATTEEKSLDRDVGSERYMAPELFESNLDKSEVNEPYECSKVDIWAIGIVLLNMVFHKNPFVVANQTDKAFCYFAGNREALFDVFPLLTPDFFQVLLFSLAIDPQHRDLNAMKEELQKIQSYTFDDDIYNKQEQDMFAHIPNVNGQKQETQIVDAVSNTNLNTNXQMLTPDPSSKKAATAAQKKDHNVIHYETQPLNISNNNNNNNNNNNTTNSVFNSNRKHYFSNIEKGKRKPLAIPTPNSHINKYFDDYKLKKQQNTFNTRDFFTPPSVKNKYLEGIFNSPNNGXTTATNKKTKVWTPSTNTAGKFHEKTRRNSGTSPYGSFNNGNGNGYTSKNNSARSINEVLDQADVVSAFTPASATDTAINTMTYHEYHHLKQQEQQQLQQQQQQQQQQQQGSRSGSTTMHNPGEEEVLFTLEEDELDSLNDDFTQFQLDEPDQVAAGSQMNTSSMNLSKNLPELLQSPPQGAHATPAMPTPSTNTFGTSLATGSFSFKERQNSSSADQNAPLVGSFNGNRTPRSSFNDSSVRSGFFTNKRPSSSQQQQLQSGYVSFKKTPKGSYVHEQSPVRYSQFGDNNGVVTDTHQTNTKKHFFSNNMNIPVNTHNTSSIKASGAASSITKPTSFREPNGPFFRQLGGYSASTVEKSDVFAKRRQSNTANISNPSAATNYYSAHFGSAANRDHDVLVFSDDDEDYLTKQHSLSNRRRSSARRSSRGPRGSVSHLHNASHLANKTYRKNSIQDDLVGSLEQYKNNWVMLQQQQQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1X5</accession>
    <name>A0A1E5R1X5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Nucleoporin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3898</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NUP186/NUP192/NUP205 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1X5">
        <property type="interactions" value="203"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1X5"/>
    <dbReference type="InParanoid" id="A0A1E5R1X5"/>
    <dbReference type="OrthoDB" id="2019644at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044611">
        <property type="term" value="C:nuclear pore inner ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021827">
        <property type="entry name" value="Nup186/Nup192/Nup205"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31344">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31344:SF0">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11894">
        <property type="entry name" value="Nup192"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ80894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1764" mass="201352" checksum="918031D2B8F86F10" modified="2017-01-18" version="1">MFWGVDQFAALYANFSDFQNGVGSVDKLLFEKLVPDLRTMLVMGNNELKNQTSRQKIESGKFTIDEEEYEVNKEFIVTVIQLSDELNCNEMIVAQILLEVMNAENDVSVQSGKLPTXFEKESSISETKLVNKAKVAFYLRRQYILQILNFLINTKTPFLQRLTKENNDYTLLDNIIPSFRNIHQQFKHIQEVVNKYQLLDQYDHFQQMNVSFKRDFLKKEYDLMAQILFGMIENGSFFNRNDDITNLVTFVTTELQSNDSFVLYYIPSLLKFPYKLHYYKNDSSVYYHFDKIIADIENNESLYTQPVKVLYYFAFLTYFISWCNEDPHNRIQKKNEDFFESKLKTPMYQLVIAGAIEQLMIIAYETSATSSGICKNSALVENEEGYDIKNLLQTYLPKLVPYQLMESNQMVYVGKALKFSPEFLDSFFIPTIHEFTQEFIANCAFLLTTLKDEEEDYMLSGETNNNDGNEQALLLETTVKKADLERFFISVYYIYKNRPALSEVFWNDNQSTAYGFIEWGAKCSDTLMRSCFFIMLSGLGSLSSTQVYQYICQNSENLATWDLLAKVLSEYIVKISKWELQNXGTEVHLNPNVLKHNTQTAQNLNSSSLPLPPAQQMHMQLPLQQQLQYQHNGSTLLPSSRFNVMNSQRMNGNYARYPSKEATNNGFYQKXSETEIGETVTISDDLSEETLIYLSSILSLISATATSLNQESKPEFSALFLDVLFEFLKLDTPLVGATLSTISSLVVPQNHTHVWLKLDRFIFESNASSSFSSGYKNYFMSKLKTLRDVTGFLKLFETLVAYKGEGFVPFGVLQVPSKLGEGNRAQYGVGPYLEYIIDEVFKNSKSLIDPKQRQYIQSIILKTIVHCLKSFDYQVILDLSASAGTNLDSLVPQKSFTEYIMESPQTFIMSHLFDESVHSILFFIVSIGFDKLVSDEETDTETSLCQDSLSILDMILTLQDTFIEEVYPIVRQQIRINSKFYVPKLFGLHGQRSFYDAILFNLSFVAHISLYVGLSNTRLASLSLSILDKISSYGASKQLVLNIFDSVDESSRIKQSFIDQLETSVETPQDFAMKLQVLRYLKNHLSAKNKPTVSHFLLGFQIAPILTTGSSVLSTFISSDVSLLKSLVCLIKKSLNYTGPLSIEYSPIKLVSESFDIIVSLCKNELTSTYTLNFLDTLDFFEFVLNKAPNVDGNTLWNGAKFSEISNPDISLATMLEFFSFRYALLHYFSLEIHRLKHTELGKSYVEFLTSSLILQSSKVFSFLDIIKHYNVQNQNGNVTEVFNTFQIYGTGISFNQVVSNLERLNNFENTAFPTIYDFTSLDSLLNLRKSWLQQCVGGNVNWNQEQSERDNIKKHITQYYSGELFNNLQLSCLHSWVQLVQVLVTDGGLSPPVRCSFIMEIFETIIPQINDLVDTNLKYSEELVSMTVFLYDIYDQDRMYNAKNFVIDKKLYGLFTTCMHGIVSPLSSIAMRSDLYAIANRFVSKLLQPNENEKLDAERIALAKDILFEMKMSSEKLVEVIASDAIAGEGPHRLTGLLLLDSLVQLAAFGKVNFVINILSRTNMLLLIIRSLKVIDDIVFSQTEANTFDSIYYELTVFKTTMNFLTHIAESKVGAHELIQNELLKILSNSNFAKLDSNLGVELLLSQQFDDNGATYVELKVKLDDSSXHYSDMNTISLFELVVPIFQLVSAILLSVGTENRMVVKNVKVLLARFEKFIQGILRKDALVEAGDYQVTVENAQNLKELVKLIIILCTLTGYEGIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R215</accession>
    <name>A0A1E5R215_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3886</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R215"/>
    <dbReference type="FunCoup" id="A0A1E5R215">
        <property type="interactions" value="751"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R215"/>
    <dbReference type="InParanoid" id="A0A1E5R215"/>
    <dbReference type="OrthoDB" id="10256606at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04330">
        <property type="entry name" value="RNAP_III_Rpc25_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000221">
        <property type="entry name" value="DNA-directed RNA polymerase III subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013238">
        <property type="entry name" value="RNA_pol_III_Rbc25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004519">
        <property type="entry name" value="RNAP_E/RPC8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005576">
        <property type="entry name" value="Rpb7-like_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00448">
        <property type="entry name" value="rpoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08292">
        <property type="entry name" value="RNA_pol_Rbc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03876">
        <property type="entry name" value="SHS2_Rpb7-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="N-terminal, heterodimerisation domain of RBP7 (RpoE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 10" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase Rpb7-like N-terminal" evidence="8">
        <location>
            <begin position="8"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase III subunit Rpc25" evidence="9">
        <location>
            <begin position="83"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="163"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="233" mass="26360" checksum="F3DD8A0F78514F60" modified="2017-01-18" version="1">MFILTKISDLVCIPPDQFHRNVKSAILYELNSKFCNKVIPKVGLALAIYDLLECDDGQLKPGDGGSYINVVFRVIVFKPFQGEILTGLVKESNSEGLKVALGNMFDDIFIPKDMMFDGCYYSPEENVWIWNTDPEDESGRVYFDLNEKIRFRVERELFFDVKPTSPKEMENPQAQQQAQAQRLKTAEGEQNGSTGNEDSEKTNVEVHQAPPPYSLIASCQTDGMGLVSWWEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R273</accession>
    <name>A0A1E5R273_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">sphinganine-1-phosphate aldolase</fullName>
            <ecNumber evidence="5">4.1.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Sphingosine-1-phosphate aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3949</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.27" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R273">
        <property type="interactions" value="740"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R273"/>
    <dbReference type="InParanoid" id="A0A1E5R273"/>
    <dbReference type="OrthoDB" id="10254570at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008117">
        <property type="term" value="F:sphinganine-1-phosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030149">
        <property type="term" value="P:sphingolipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3090">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050477">
        <property type="entry name" value="GrpII_AminoAcid_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42735">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42735:SF6">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE LYASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="266"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="266"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="326"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="369"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="412"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7">
        <location>
            <position position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ81005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="854" mass="94472" checksum="2A3E6A5060123D0A" modified="2017-01-18" version="1">MDPLLHLQEYLDTTSRWTVFKDIVFYYFLVSNVFIPFLYFIISPKRQRKVQKKLWDFIVLTLFHNSTQHELDKLRKTLAEKLIVNDPKIHIYHSLPKIGMSHKQVMEYMKEQCDVVDKGYAKVENGRVSGAVYHGGKELINLQSQVYELYCVSNQLHPDCFMSLRNIESEVVEMVLRLFTMSRAKKASTNRTTTNISTRNISTRNISTPNISTPNISTPNISTPNISTPNISTPNICTPNISTPNISTPNISTPNICTPNISTPNITTPNISTPNISTPNISTPNITTPNISTPIEKPNISTPIEKPNISTPIEKPNISTPIEKPNISTPTIDPGSSTLNVGKPNSDDLDTTTLKNVSPEIHISAGKQSDAEKPSVRASEIEKPTIETSNGENSDAPVPDFENPASVPANEHSINPHSANSNADSLNEKHMTDTVQSNTPLSKPKTSKSLLFQLKSVFRTQLHTGDYTYCGTTSSGGTESILLACLSARNFFLKRHLVTPTQLPQLIIPETAHAAFYKAAEYFHMELVVVPVRAEMGYRVDARDVARCVTSKTAMIVGSAPNFPHGIVDDIQGLSRIAIKWDIPLHVDCCLGSLLMPFYAETKGYEPFDFSVPGVTSISCDTHKYGYTPKGSSVIMYKYEVLRSAQYYIQTEWSGGLYGSPTLQGSRPGALVANCFSTLLNFGENGYRESFKRISSTVMEVAQFVRKDPLVSKYLEVIGDPKICVVAFKCKTGNGXFFNESDIYMIGDVLQNKFDYHLSSLQHPAALHYAITYLASSRENVASFMEALKQSVEIVVAEAKQNVINSAKDTTKKKKTDADTAQLYGVAGSIKTNGVAGKIVESFLDTMYKPVPNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2F1</accession>
    <name>A0A1E5R2F1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transcription factor HMS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4117</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2F1"/>
    <dbReference type="InParanoid" id="A0A1E5R2F1"/>
    <dbReference type="OrthoDB" id="3973303at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052099">
        <property type="entry name" value="Regulatory_TF_Diverse"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47336">
        <property type="entry name" value="TRANSCRIPTION FACTOR HMS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47336:SF2">
        <property type="entry name" value="TRANSCRIPTION FACTOR HMS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="2">
        <location>
            <begin position="402"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="528"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="645"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="528"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="599"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="645"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="682"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="3">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ81058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="694" mass="76305" checksum="8174C3A4FC922B38" modified="2017-01-18" version="1">MNGYFAEADKFLASLQESLNNGSDLGLSSPSTMNMNNMADGNIGVSTTNINDNNNNNNNNNNNNNNNNFNVASVGNMNRKNNKSGLGRASTYHSTKLDQRSHSNLNANNIAALSNVHGLNNSGNTGASNAVYNPHIHPGHVSKHYNSAGLDRRSNTYDNAFGRLHNKAMFNSTDLDFGLDLDYLXNDDEEKEEEEEEEEEEKQDXNENDINNNINPAQDLGSRSNTISFQFNNQMSSMNNTLEPLANSSFPVGSITNISGQPNTTPMSKNNSINATIKGNKNSDGTAMVKQESYLFNDLLNAPQKVDIQNNQNQLQNQXQSQGQYKXLGQNGVPNPVISALSTNDFDLRQGSLLDEDFDDDIGSFDENELLDDVNNLASSSLSASTLPGAAGLANQPKRKKKEKVSHNIIEKKYRNNINEKILTFRNIVPTLRVSHKRERGDLILENDYFLLEGLEPAKKLNKATILAKTIEYIEHLETKCEILTDQNMQLKNIINSSTTNASTPRNTNFNSYQNAGQQNHLKNLSAKSMSQNNSISSTHSRTNINNSNTPNHFNTGNTYNMDPSQMMGNSGMNMKTSFSNMSNPNAQGPPQNHXNMYSSQTTPASASSYSSGNSNVLGSGGSNNGSTVVSATYQRNQAISPVANXSNVSNTSNMVSPNSINQQQQQQQQQQQQQQQQQQQRXQGGNSMMFNXS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2H5</accession>
    <name>A0A1E5R2H5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Condensin complex subunit 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g4109</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CND3 (condensin subunit 3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2H5"/>
    <dbReference type="FunCoup" id="A0A1E5R2H5">
        <property type="interactions" value="245"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2H5"/>
    <dbReference type="InParanoid" id="A0A1E5R2H5"/>
    <dbReference type="OrthoDB" id="27187at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000793">
        <property type="term" value="C:condensed chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027165">
        <property type="entry name" value="CND3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025977">
        <property type="entry name" value="Cnd3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14418:SF5">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14418">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12719">
        <property type="entry name" value="Cnd3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nuclear condensin complex subunit 3 C-terminal" evidence="9">
        <location>
            <begin position="595"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="200"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="507"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="989"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="211"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="527"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="995"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1031"/>
            <end position="1040"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ81101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1040" mass="118066" checksum="17AC7D5552D520D8" modified="2017-01-18" version="1">MSTCVSPNTSIDGQSTLVEHQIFKSLAEIFKESQHTYAGHRRNIALLKKIQGKAKGQGLEDVFNFMFCKITTKILPLKKAQVEGDRVVKLIAGFIASWERHVIKINAETQNDNNEEATAFSTFLNKFMRYTLRGIESRDRNVRYRVTQLIAVIMDNISEIDEDLFDILSWAVFKRVYDKDPNVRIQAIFSVTKFQQDDTQGDENLINEGENQQEDREEEDGENINADSTNATKILVKLMRNDSSPEVRRACMLNLVQTEQTLPYILERVRDSNAINRRLVYSRILKNMTNVFQVIEKSVLDKLINWGLNDREDTVRNACIKLVTIHWLNFVNGDLVDLLTKLDVMKSDVAAKIIEQLFETRSEVIEKIKFPKEIWGCFTPEVAFLLKCLFNYCHAKKLDTIIDENYLDTVEFASILSKYVSKGLSKTAPDFLFISQQLLEIAKNYDYSDEVGRRDMLNLIRNLLYTRNLNEECTRLCVGVLHTLAISEKDFIMIVLEIIQDLRDESFDDQEQARQKNKKKRKRAENNNEDDSTLEPDEEDDELSNEDGDDSSAIESFAAAVDDLVRNGQESASPKEAETPALLNDDALDGKILIKCLTVSRCMLELVSEQTESIIFSSLIETVITPAVRNSDSDIRNLGLRNLGLCCLLDIDLAAENMYILGMCASKGNSSLKAIALQIIVDIFSTHGTKVADGEGKVDLVAVHKIFYKILKNTDLPECQSIAAEGLCKLFLGNVIVDDDLFETLILSYFSPANQDNEPLIQAFAFCLPVYCFSHPEHQEKMCNIAPDVLFRLAMLWTDLKAGDGGSDSMLKPNVIFQQLIHWTDPAQVVGQTSDAFLKANYQLEFLKNLLQIYDRFERKEVKKMILTNVNRFFVCKEKSLSLMQDIRLKVCEILESDLTDTISSNSMKKFLANLDVCIEENNLANDILAKEISAVNENPENVDSTLFEHNLEQHEKNKAIIGKEEPIATNKNQPIAPDVLSSIDSTNLEEEENHENRNVLKPNETRKPVSGGKSVDFILPDLSDNEKDTSSMNDTSYDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2M7</accession>
    <name>A0A1E5R2M7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exocyst complex component SEC15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g4359</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the SEC15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2M7">
        <property type="interactions" value="555"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2M7"/>
    <dbReference type="InParanoid" id="A0A1E5R2M7"/>
    <dbReference type="OrthoDB" id="10267033at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090522">
        <property type="term" value="P:vesicle tethering involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.670">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.30">
        <property type="entry name" value="Exocyst complex subunit Sec15 C-terminal domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007225">
        <property type="entry name" value="EXOC6/Sec15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046361">
        <property type="entry name" value="EXOC6/Sec15_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042045">
        <property type="entry name" value="EXOC6/Sec15_C_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048359">
        <property type="entry name" value="EXOC6_Sec15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042044">
        <property type="entry name" value="EXOC6PINT-1/Sec15/Tip20_C_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12702:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12702">
        <property type="entry name" value="SEC15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20651">
        <property type="entry name" value="EXOC6_Sec15_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04091">
        <property type="entry name" value="Sec15_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025007">
        <property type="entry name" value="Sec15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 5" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component EXOC6/Sec15 N-terminal" evidence="8">
        <location>
            <begin position="85"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex subunit EXOC6/Sec15 C-terminal" evidence="7">
        <location>
            <begin position="472"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="778"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="888"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="778"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="888"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="918"/>
            <end position="929"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR025007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="929" mass="107709" checksum="44A68B846F8EAE4F" modified="2017-01-18" version="1">MDQQEDSGVVTLSKDLQSILFHTEFVNHDQKTYQNGLSTLENGSAQDMTSSVQLLDVEPQSLDRWVPEIRKFIEDDNLDDVLDELQKSIQDNFQDANMDFFQNPQINDQLMNSIENLSKIQTQLHNTQLQEQLRGLQKDLSQYTEKVIFHKQLFLQNKKTNSKIKEATILINKILQILELSNNCHQLILSNQFFRALQSLDSLEIIYSQDFRKEFNFDFLSQFYQSIPLFKEIIRDEXINLIKKFFNSNLEKVMDKYGSQVFESYQKGYYQDWLAQKAQLKLPSAIKFNSSVEISLRDDAKQRLLSPAKWFDLEAFNDAILIFTSMNQMSYLSVEFGKIYEFWENKFVYPLEFVKNSVAVNLSQQQQKVQERQKYSIFKTVSDIKGYLLKITGFLVFDKFLQRETDYTLTEDAFTSTAYFWETFCKNFLPYLKFFITYQLSTKDLKSLNEMNQVMGIFISIFSNLKLDTKSFYTCHVQLFEKYCXYEKGLIGSEFTQLLKDDDFMPLSLNDKALYSKVMKLCWLKESDKEVLNDSLLSLPDEEFSVALPFSPLYPMTCSLLKKSETRLHKFVEEFFPFQTSLIYEKIINSVDSIFQDVIIVQFEEKLGSKSREELLQVLINLDYFYISTIEMAKILSHVSDQEVQLSATNKMRKVRDLAETKLIELIDSKVSDLMEIIDLDWTAQSVRNEPDLIISDIADYLYNMFTSTLANLPYQMKTLIIFREFDALTSRFLNMLVYGSPATITPQSVLNFETDINCLEDVIASIFGASNSADSGLSSNDSSSAQLQAPPTPTIGRESSIPFGARSPSMFFTSDLNSTSMESNIRSLRSTFDDLKQHIRLLKSGSLEQYKTARMKIYPRVKPEVANMLINKLSTYQLMLRERQEQLLQSEDSSDSSSVRKVSASNNDDSMSLMSSRNKKKIAKFFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4I5</accession>
    <name>A0A1E5R4I5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">mRNA-decapping enzyme subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3496</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DCP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4I5">
        <property type="interactions" value="164"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4I5"/>
    <dbReference type="InParanoid" id="A0A1E5R4I5"/>
    <dbReference type="OrthoDB" id="440673at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010334">
        <property type="entry name" value="Dcp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16290:SF0">
        <property type="entry name" value="DECAPPING PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16290">
        <property type="entry name" value="TRANSCRIPTION FACTOR SMIF DECAPPING ENZYME DCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06058">
        <property type="entry name" value="DCP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ81829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="233" mass="26295" checksum="EFF4ECCE020F344B" modified="2017-01-18" version="1">MNEGETLDLYRKALNYNVIGRYDPKIKQLLYHTPHAVLYRYNVAAESWEKQDTQGVLAIYLRNVNGDVPDQLSKVEDGTLLTNATADGTTNPMDSTASMSTLIDEAIQAKDKLAGAXDDSEDTIKILQGEDIYNYGLILLNRSNPENFSLGIVPNSVVNKRRIFHKNHNSAATTNISDXELNNMQIEKNDGLLIIKSLLGQVFGLWIYNEKDRETLFTLIKYLLESEPRDSFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4V6</accession>
    <name>A0A1E5R4V6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mitochondrial nicotinamide adenine dinucleotide transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3696</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4V6">
        <property type="interactions" value="465"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R4V6"/>
    <dbReference type="OrthoDB" id="3971108at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015218">
        <property type="term" value="F:pyrimidine nucleotide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990519">
        <property type="term" value="P:pyrimidine nucleotide import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049562">
        <property type="entry name" value="SLC25A33/36-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45829:SF1">
        <property type="entry name" value="CARRIER PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G06780)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45829">
        <property type="entry name" value="MITOCHONDRIAL CARRIER PROTEIN RIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="21"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="140"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="291"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ81922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="383" mass="43326" checksum="997B411293A279B1" modified="2017-01-18" version="1">MNTFNTFKNKENIPLITRNLYSTEVVALSGALSGFLAGIMVCPLDVAKTRLQAQDNKLAKTKSLSNFNTSSAPHATKTFAKRKYNGLLGTLKTIVKEENIKGLYKGLVPITLGYFPTWMIYFSIYEHCKHSPFYQSHLGQDYQLHSLSAMTAGLVSTVLTNPIWVVKTRLMLQTENINKNVVVEVLPVFAPSKIINNNNNNSSNKVLIDNYANKYKGTLDCFIKMXQYEGWRSFYKGLLPSFFGLFHVAIHFPLYEKCKAILHVNASAQNVLPINDNESPQHSPIMSSKDQYLGKLLLASCISKMIASTITYPHEILRTRMQVRNSQSLVKTIRKIYKTNGGFKGFYQGFFTNLIRTVPASAITLVSFEYFKSFITKHNTHFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R528</accession>
    <name>A0A1E5R528_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Inorganic pyrophosphatase</fullName>
            <ecNumber evidence="4">3.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Pyrophosphate phospho-hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3735</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>diphosphate + H2O = 2 phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R528"/>
    <dbReference type="FunCoup" id="A0A1E5R528">
        <property type="interactions" value="1019"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R528"/>
    <dbReference type="InParanoid" id="A0A1E5R528"/>
    <dbReference type="OrthoDB" id="1608002at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00412">
        <property type="entry name" value="pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.80.10:FF:000004">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="INORGANIC PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00387">
        <property type="entry name" value="PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ81999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="287" mass="32478" checksum="D4A073C0CE90189A" modified="2017-01-18" version="1">MAYTTRQVGPKYSLDYKLYIEKDGKPVSPFHDIPLYANEEEQIFNMVVEIPRWSNAKLEITKEEALNPIIQDTKKGKLRFVRNCFPHHGYIHNYGAFPQTWEDPNEVHPETKAVGDNDPLDVLEIGETIAYTGQVKQVKVLGVMALLDEGETDWKVIVIDVTDPLASKLNDIEDVEKHMPGLLRATNEWFRIYKIPDGKPENQFAFSGEAKNKKYALDVIKECNEAWKKLISGKADAGKIDLSNTTLKDTPTYVASAESLNLPKAGEFKDEPIDKSVDKWFFISGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R554</accession>
    <name>A0A1E5R554_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sedoheptulose 1,7-bisphosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3718</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R554"/>
    <dbReference type="FunCoup" id="A0A1E5R554">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R554"/>
    <dbReference type="InParanoid" id="A0A1E5R554"/>
    <dbReference type="OrthoDB" id="4818801at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050278">
        <property type="term" value="F:sedoheptulose-bisphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046390">
        <property type="term" value="P:ribose phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050275">
        <property type="entry name" value="PGM_Phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100">
        <property type="entry name" value="BROAD-SPECIFICITY PHOSPHATASE YOR283W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100:SF15">
        <property type="entry name" value="SEDOHEPTULOSE 1,7-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="1">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="23"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="100"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="279" mass="32008" checksum="1485FDCE18C92D04" modified="2017-01-18" version="1">MVTPRCIVVRHGQTEWSKNGNFTGVSDIPLTQYGISQVHATANALFEDPEAPTYLDPNHVTYIFTSPRSRATHTKDIFLEHIPEKHRSQIKVIVDDDLREWEYGDYEGKLTHEIIALRKSRGLDQEKPWNIWRDGCENGETTEQVGCRLSHVIARIQHIHSIHLNQNKPCDILVFAHGHTLRYFSSLWFMSMFSSGIVQNKLVSTNFKPQTNYVGFQNITKEEAERDVPFVALEKFYYLNDNPWFMLDAGGVAALSYSHNKISEPSLMLSGIFNPRATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5L1</accession>
    <name>A0A1E5R5L1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Methylsterol monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3623</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R5L1"/>
    <dbReference type="FunCoup" id="A0A1E5R5L1">
        <property type="interactions" value="302"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5L1"/>
    <dbReference type="InParanoid" id="A0A1E5R5L1"/>
    <dbReference type="OrthoDB" id="1658724at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="5">
        <location>
            <begin position="155"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ82158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="316" mass="37469" checksum="6907C0E629A11A29" modified="2017-01-18" version="1">MSIAAAVFPNNSTLNNLLTNVDGTKSFSQVLNNVGQMQPQLSLMEKYWASWYCYMNNDILATGIMFFVLHEFMYFSRSLPWYIIDCIPYFRKWKLQPTKIPSNKEHWHCLKSVLLSHFLVEAIPIWTFHPMCEKLGITMEVPFPNWKTMTAEISLFFVLEDMWHYWAHRLFHYGIFYKYIHKQHHRYAAPFGLCAEYAHPAETLSLGFGTVGMPILYVLYTGKLHLFTLCVWITMRLFQAVDSHSGYDFPWSLNKFMPFWAGAEHHDLHHHYFIGNYASSFRWWDYTLDTEAGPEAKLEREERMKRKAEEKVKKTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7E7</accession>
    <name>A0A1E5R7E7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R7E7"/>
    <dbReference type="OrthoDB" id="4070605at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="466"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="687"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="742"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="470"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="496"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="695"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="743"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="770"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="2">
        <location>
            <position position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="991" mass="111466" checksum="FF1C9BB1FBF62604" modified="2017-01-18" version="1">MEFNKVKLDRLDRLFLLSYHHKNDQKNKLNVQDSPDPVTRKEXLEETAKCTNGNIKCXTKNKVPKNQVTPMETLKNVGARRGSGTAITFGSERIGKCTQNMNVKPIKMFIDLPTIDYYNDELAHNKIMKFNKKLKVHTLRDFILSEFQIPELKLPSKNVNQEKTTRDDEYASLPAKDRKSFDDKIFYDITIKSSGSLRYVGTIKYIIEECFSKLSENRCRFHIQVTQTSLKWFNTYLNRDILALSSKFIEYKVINNNLIYSDGHSDVALTPFKEYYKELSRLFTSKILPKTNTNESWQAQSENGTWDECIIVVTNNTGIKALLTILSDRPLTKFLTENSLNALSSDPNSEFGMSSKHLERSSTPPLAAGNVSWVPXFPNNFTPGQTTKSGTVTKRNVPTNFDNITSYKDKSVKVLSSENLAKLIKPRSSNLSTPLEKKVLLKNTRKKIHHTDELNLSDVLNAEQNDKDANYNNDNNDNNNAHEDEEEEEKDQKTNDEEDGDEDEDDEDDDSCSDNSSSSSDSDDSGLTLNVPTYSQRMSLFDFNPRTSGRARSISLMGPAQQAPFLTKTQLDSENQTIXQKSAGETHTQWVDEIXDFKAKAQTTEFYDDRLTEENLEDEMCSAKKNFQNSHYQNIYVHDGEATDDSNDIDEVLFNVAQDGNNLSRKSSTXSFATLPGSVRVSRAKNLKNSNNKLNRFRTRSRSRSKHRSLSLSQSKTNNSSANSLIPPEFYSKISAASPIPVRSSSELTTQENSELSLKQSRPPDGETESAGTSDETVNSSTKANPVDLNLLSRGDFGKYLTTGNKDLLIVRKEQSPQRKMSFDYEDXQANDHYALMFGSSNAKSGIKRDHPDNGGQELEISNKLDGNNHEKAINMPFDKNITLPRTFTGLIDLKNLNLYSQTAQLESEVXNGGPAANTDVLDVEDLLMCGTESVKPGLNDDHAXFSLETSSSNQNEKQEPKIVCAKPAFTLDLYGDGETDSNAWVFGANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7F1</accession>
    <name>A0A1E5R7F1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Phosphatidylinositol 3-kinase VPS34</fullName>
            <ecNumber evidence="4 12">2.7.1.137</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g3162</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12709"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.137"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:12710"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.137" evidence="4 12"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7F1">
        <property type="interactions" value="856"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7F1"/>
    <dbReference type="InParanoid" id="A0A1E5R7F1"/>
    <dbReference type="OrthoDB" id="67688at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016303">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00896">
        <property type="entry name" value="PI3Kc_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 3-kinase catalytic subunit type 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000016">
        <property type="entry name" value="Phosphatidylinositol 3-kinase catalytic subunit type 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057756">
        <property type="entry name" value="PI3-kinase_type3/VPS34_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002420">
        <property type="entry name" value="PI3K-type_C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008290">
        <property type="entry name" value="PI3K_Vps34"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF7">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE CATALYTIC SUBUNIT TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00792">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000587">
        <property type="entry name" value="PI3K_Vps34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00142">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51547">
        <property type="entry name" value="C2_PI3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="C2 PI3K-type" evidence="16">
        <location>
            <begin position="35"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="PIK helical" evidence="15">
        <location>
            <begin position="304"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="14">
        <location>
            <begin position="653"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="495"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ82826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="961" mass="109696" checksum="56172F49B4D722F7" modified="2017-01-18" version="1">MSDSKDSVTFCLLSELSSISLKIHIDKLIGRKPLLTQSARIQSPERTRINSDVNLTSELFVTVQVYDXFTNRELSTTVTTNYKPFKNTRKWAQWLEIPIALSLLNAHSKLLITLWEYGPEGSKIIFHKLDSGLVDQDSMTLKSGSECIKFDLSASYGNHQIVHNESQKLLDKYIMGEIAPEPWLDHLSLDKLKKQSVQFDLKPNEFVLFIHYPYYEVPIVVDEKELPIYSNIPTIESFKLNTKEVLTNSEKNKQNKEQDLKQESIQLPADYNSLKFFDPDQYNPDPVEEKFRRLERSSNKNFNFTAASKPDASKREILNKIINYPPGTELTAHEKALIWKYRYYCMSSKRALTKFLQSATLNDENEKTEVIRLMDLWIEIGIEDAIELLGPRYTLPFVREYAVRRLEKSTDDEIQLYLLQLVQALCLENVPYNFQEQKLWKGTSEQMGQSXILDTADLTIKHDSLTNTTNSPKVPKVPKVKQDLLVSEYPSSTLDGNNADFDADSNGEASFPANRYDGEDISFSSVADPRKPEPAANSSLASFLIKRAVANEKLGNYFFWYLKSESKDSANLLIILEAFFESLTDQQKEKVISQVTFVDMLRDFCIQIKSLRDTTQKKTSLLHHLVTHRLKPLLRQKIVHLPLDPSIIVTDVIPNQCKVFKSSLSPLKITFKTKDNKSYSLMYKVGDDLRQDQLVVQIIKLMDVLLKNDNVDLKLTPYKIVTTGPDEGAIEFIPNETISNILSKYHGIASFLANYKPDQTTKTSTVNPSRTLSKDSDVTLATKINVKGLEIDHTVMDNFIKSCAGYCVITYILGVGDRHLDNLLITPEGRFFHADFGYILGRDPKPFPPLMKLPPQVIDAFGGADSPNYNKFRSYCFVAYSILRKNAGLILNLFELMKNSQIPDIKLDPESAVLKVKEKFCLDMSEEEAIIHFQNLINVSVNALLPLVIDHLHNLTQYWRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7N4</accession>
    <name>A0A1E5R7N4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3428</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R7N4"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014804">
        <property type="entry name" value="Pet20-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08692">
        <property type="entry name" value="Pet20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="13"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="4">
        <location>
            <position position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ82922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="339" mass="38904" checksum="A7EA77D53ADB96BC" modified="2017-01-18" version="1">MMMRTKPRFCHSTLETFKRLQSSSTSSKTHVEVKEEEQLSAGKQKKSRKSRSHDEPGSATXQPSSSLSSTPKCVKVKVGQNSSAATPRTKVKKKFVKFPRVAKLDDKFSNQNCNLDILYSGYKPLFRAHEFSNQQQSLQDKLLGIPKKSTSSSMQNTVNDLLGDKNHQAVANAGNQENMQNLQININIDRDSDDVKFPKNFKDLITNFTPYQNQKIIALRKYAKFTPWCSSAANMEWYRDWEILPDTLLKEMKPYQNPTQFDEPLIKNKINKNLELVPVKKDFSSTDLASXNASNHDKSEDVYDLKLATYRKEKENYNKKGKGRKKPILKLMKLKKTLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7S3</accession>
    <name>A0A1E5R7S3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-Gid-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3248</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7S3"/>
    <dbReference type="InParanoid" id="A0A1E5R7S3"/>
    <dbReference type="OrthoDB" id="1933281at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044063">
        <property type="entry name" value="ZF_RING_GID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51867">
        <property type="entry name" value="ZF_RING_GID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-Gid-type" evidence="3">
        <location>
            <begin position="457"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="RING-Gid-type" evidence="1">
        <location>
            <begin position="457"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="89"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="4">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ82962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="517" mass="60526" checksum="EBD3966EDEC24D00" modified="2017-01-18" version="1">MGVKTVDIYKIEEDLESIHKTVLPSLNATIEKVHEQRLNVLKVRAHLKKLSEEKEAKTPQEYASRKLKIEKNLVNNLKQYLVSDVVPQSSYHHDDSSAHHNYHHHHHHNHHHHPPHHHHPHDQQQHHEHDDKPVFANFAQLQEEYSKRMLDFAKMCSEQHSTLENLCLNQVDVDQETELMPLISQHLLFCGITRNSSWNFNQHKLPPNMPVSSFDTVGKLQSMLEDYCERGDDTXIVGFLDEMTENFTLSRYRPNSFKFILPRNSNXSNPSLFKIYVKLIQSQILFYKNQKQKAMHALRTGTHFLELFKKTNTYERKDSEYIENLEKEYSSFNIRLNNSFDNTIYLRNSSLVESLLLSIQDVVQTFSIFYFYSHDMKPNSLLEQCCYAGIFTLMSIFTNIEDHQFMVFKRLGNRLESKIDQEKGSKESLYSWYEDQDANFEVVLPKSLQKYHPNFLCPVARTQFDPVNDPPKALPCGHILSARTVSQMMTCGSLRCPYCTVFSSKDEIKSIQFFSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7Y3</accession>
    <name>A0A1E5R7Y3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3279</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7Y3"/>
    <dbReference type="InParanoid" id="A0A1E5R7Y3"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="104" mass="11809" checksum="1B5BA97F6DD86CCD" modified="2017-01-18" version="1">MLTRFTNLKTTAYVAKNITKKRSLHTTFNKKPSSDLNNSGSMVTNLYVFYLSLGFTIPFLISYQASQDRVNNLLTNDGLEKNMSEGKDICAECQFEMDPKRNNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R846</accession>
    <name>A0A1E5R846_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">1,3-beta-glucanosyltransferase</fullName>
            <ecNumber evidence="7">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3476</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Lipid-anchor</topology>
            <topology evidence="7">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 72 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R846"/>
    <dbReference type="FunCoup" id="A0A1E5R846">
        <property type="interactions" value="89"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R846"/>
    <dbReference type="OrthoDB" id="421038at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071970">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004886">
        <property type="entry name" value="Glucanosyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468:SF4">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03198">
        <property type="entry name" value="Glyco_hydro_72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 7" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="7 9" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009028098" description="1,3-beta-glucanosyltransferase" evidence="7">
        <location>
            <begin position="26"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="483"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="483"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ83041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="534" mass="58291" checksum="00833133A6E82F10" modified="2017-01-18" version="1">MKGSLFNKLAVSLTTMLCLMQCCYALPPLHINGTRFIKPNASRNDPETNEVFLIKGLDYQIGGSSAYDPNSGSDALTDEGVCLRDAFVFQQLGINTIRVYTLNPDLDHDKCFTILNNAGIYVLLDVNGPLYGENLDRSNPSGTYDSTYLTRVFKFLENFKNYPNVLGFFSGNEVINDQDDYAYIDPPYIRAVQRDIRQYLSAHPPSNRSSTIPIGYSAADNDDIRLPSYQYLQCNSLDGKTVDTDLAESALEFYGINSYAWGNDATWTTSGYNILNETFGKYLGRPIFFSEFGSIAQTPRTFNQISQALYNEDNGLLNTFSGGLVYEYSSEANNYGLVSVDSDDGSVTYQDDFVNLQKQYASLKLQPTKEADLEAPETSTEIIQCVPTAVTSEYASFGVQNFSIPAQPTEIASLIKNGVSANHTGAILTDYTPASTFSYKIYDTDTKEIDAKISYPSSNQLNAVYATSTSSSSSVLKSASSISSSSSSSSSSSSSSSAQSSSSSSKKSKNIANSNNNLQYGLLSVVFGGLLTFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R848</accession>
    <name>A0A1E5R848_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Flo11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3307</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R848"/>
    <dbReference type="InParanoid" id="A0A1E5R848"/>
    <dbReference type="OrthoDB" id="4070545at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018789">
        <property type="entry name" value="Flo11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10182">
        <property type="entry name" value="Flo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01213">
        <property type="entry name" value="Flo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51824">
        <property type="entry name" value="FLO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184668" description="Flo11 domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Flo11" evidence="2">
        <location>
            <begin position="20"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ83082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="343" mass="38020" checksum="28C0BC38F2654025" modified="2017-01-18" version="1">MLLNHFILRVFALIPLIAQLVSAGATCTPFKQCQQNNMWYFQNVGGMPYYFELTRVEYLSGTSYSITFHVWSDANIPLTSLNELKFLGSGIQSSDVMIYSLNTGAGLNDPNFNPTDFTKTITVDTSSANYGSSLTGLPAGFGFQFDYCKGMNTYSTPKNSAFYSGYDYTCQCDWTYGATSFDYYTSCNGDNNCNSQAAFSNWLWPKQCGSLSDQTKKSQISSLSAANPYSYCTYAGTPKADFTSYFKVWWTPDACKSYTPTKTTAKTTIPTTPTTIKTTTSACTTTKKTTTKKKNTKKTTKKKTRTPECTTTKKTTPTPTPTPRPSCTENFFCDKLFQGACDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R890</accession>
    <name>A0A1E5R890_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Abhydrolase domain-containing protein IMO32</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3331</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R890"/>
    <dbReference type="ESTHER" id="9asco-a0a1e5r890">
        <property type="family name" value="ABHD11-Acetyl_transferase"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R890"/>
    <dbReference type="OrthoDB" id="8119704at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46118">
        <property type="entry name" value="PROTEIN ABHD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46118:SF4">
        <property type="entry name" value="PROTEIN ABHD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="66"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ83117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="363" mass="40958" checksum="CB6A46FADDE3BF3E" modified="2017-01-18" version="1">MLSTKMLSTTSRNMLLGNMRLMSTAGPATTSTLKPLPEKPLVEMAYDVHLPERSVIGKLPYHVQEPVVFVHGLYGSKRNYKTDAKKIATYLQTPVYVVDVRNHGQTEHALPFDYTTLSNDLIHFIKKHELGXVNLIGYSLGAKISMLTMLRNPEMVKAACIIDNSPIVQPEIVPFLKIFTKACVDTLTKANIKATDKSWRHKASENLKKFIPNAGIRGYLLNNVINHKPKGKDYKNPLVNYDDGKIHWKNPIQHMTKVAVDDVAAWPVSATEGKKYLGPVGFIRGTKSDFVRANGLKAINEYFPYNKVIDINATHFILNERPSEYVRDVCDFFKTMRPALIAKRTGDEAHMTDSQVTEKRLHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R895</accession>
    <name>A0A1E5R895_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptide:N-glycanase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3324</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transglutaminase-like superfamily. PNGase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R895"/>
    <dbReference type="FunCoup" id="A0A1E5R895">
        <property type="interactions" value="107"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R895"/>
    <dbReference type="InParanoid" id="A0A1E5R895"/>
    <dbReference type="OrthoDB" id="409136at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000224">
        <property type="term" value="F:peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006516">
        <property type="term" value="P:glycoprotein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.620.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050883">
        <property type="entry name" value="PNGase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12143">
        <property type="entry name" value="PEPTIDE N-GLYCANASE PNGASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12143:SF19">
        <property type="entry name" value="PEPTIDE-N(4)-(N-ACETYL-BETA-GLUCOSAMINYL)ASPARAGINE AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Transglutaminase-like" evidence="5">
        <location>
            <begin position="174"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ83132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="348" mass="40390" checksum="0FCCA693A31A7503" modified="2017-01-18" version="1">MSSTEPSHSIAEMAAQLLGKYKNLIIGKYQEQADSYRLHQLMSNNRFAQQIDGLHKENCFKYDADPNIFTAVMDTIDLDLIYSNVDKNEESEQDYEKALVKELLRYFKEDFFTWTNQPNCTQCGNNSNQQFKSADRPTPEEQSKDRLLSLVEVYQCTKCQTITRFPRYNSPYMLLKTKTGRCGEWANMFLFLLKSFGITSRYIWNREDHVWSEYYNDKEKRWIHLDSCEKSYDQPYIYSKNWNKKMSYCFAFGNDAVADVSAKYIVVKDNALPRTEIEEADLSFLLFELTKSLRNGLSADELYKLGCRDQLELLELEGEEVVTKTSTAPLGRVSGNAAWKNARGEGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA03</accession>
    <name>A0A1E5RA03_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3003</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA03"/>
    <dbReference type="InParanoid" id="A0A1E5RA03"/>
    <dbReference type="OrthoDB" id="3971417at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF733">
        <property type="entry name" value="RE52890P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="7">
        <location>
            <begin position="488"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="287"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="372"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="433"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="541"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="951"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="56"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="94"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="138"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="195"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="308"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="372"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="437"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="553"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="952"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="968"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="979"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1003"/>
            <end position="1017"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ83736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1017" mass="114051" checksum="549FA81128938048" modified="2017-01-18" version="1">MGLRRWIASNSNNNSTVSLNDLDNENNKNKPEAGNKFYKSGISTDYESDSGDRGSKSRNVKGKKKRSMLKGLRSSFIGGSNSASENESSDDDTNTNNDNNGIGKKNSHFSLFSQSKNSSQSLSTVKNEFEDADDYDERPEAQGEEKQQQEKEEEEEKEEEEKEEEEKEEEEKKEQDVQNKGATSAEGSSLAKXIXTGSSSTNGTLTNERYATNGKEMLNSNINNSAENDNENQFXSKNLDFDVETSKVSKYKTKNGSKLKNEITKNDFELGEKDKASNDIXIIDEQKTIKDVSSSDSSSQEMERGEHATSSADSSRGTTMDNSMSLSKHSDLSSRTNSAGLWLPSDPIAVPKPKTLVQSAGSTMLNLSKIPSASAPATPTGNNSGIGANLLRKSGMNSTSHHHDSDHINHSTSHNGRVHKMMHGVAHKFAFDESHNNNHSHXNNNXNNNNTNNNTNNNTNNHNNHNNANNHSGTSKKAEVSTNSEKVFGLENFGNTCYFNSMVQCLFSIDEFRIQMMTPLIPEDNHDRKLHCYPLKPRYFTKQSLTKNKQKDSGSSNNSSGANNGYKVISTDDVLAKLNTSYDKIIVGRSQVDKKRHSSXTRKRNALIKGPVVNIDHLSTNYHHTTMGWEKDDSKIMYYCLKDLFEYIIENKYYTGVASPIKFLNSLKIVNCMFSTSMHQDAQEFSNFLLNSISEYLDAYHHKNFVKYLFQGEIVNKTKCLKCNNITFRNEPFLEFSVNISKKYKDLQSYFDNYYEKESLCGANKFHCDNCQSLEDAERVAEIGSLPKILVLHEKRYKYSEKENTMIKLFDKFEYSRNLKVCTTLSNSTNDPNEEPVNVCKKYELNSIVIHLGGGPSYGHYISIVKNSALGWLLYDDESVESIDEKDVFKFAGSHEDLSAAYVLFYKEVEAGKTADPDDEKSRNKQYQKNVANFIKKDELMRFRIDESRKNSVSSSASMDSMTTSGSLMSNASGKTNQSNSSSSSSSKSKIKRRGTXLFSGFKKNNNSSATSIKEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA14</accession>
    <name>A0A1E5RA14_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein AST1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RA14"/>
    <dbReference type="FunCoup" id="A0A1E5RA14">
        <property type="interactions" value="81"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA14"/>
    <dbReference type="InParanoid" id="A0A1E5RA14"/>
    <dbReference type="OrthoDB" id="201656at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052585">
        <property type="entry name" value="Lipid_raft_assoc_Zn_ADH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43482">
        <property type="entry name" value="PROTEIN AST1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43482:SF1">
        <property type="entry name" value="PROTEIN AST1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13602">
        <property type="entry name" value="ADH_zinc_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="1">
        <location>
            <begin position="42"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="422" mass="47936" checksum="661C402B7F848DB4" modified="2017-01-18" version="1">MSEQGWTDPKILEQPLELHRVARPLRPVKYVPTKSIVFYSKNSAPQFTYDIKIKMPIKATSVIVQVLYAALNPVDLKIYNGYTTNMNSFTGLGREFVGKITHLGKNVKDYQIDDVVMGTFFHPHLGKGTLQSSIELDVNKDVFFLKPEGLSLQEAAGGSYALGAAYSLLSTVKQLQNNTNDDVNILIYGGGSSVGMFALQILKYHYKIASKITIICSAYATNVIKAQFPELIDELIFINYLSFAKNKPSKTLSAMIQDQEIIEYVNTPGSTQTAVLSVPYTQGKYDLVLDFVGGYDLIENSSKIMNKNAEYITTVGDYKFNYRKDVYAFNNLQKNNLEMFGRSLWSKMSWDFQYTHFQFDPALKYNTSYYNDWKTHVHDMLEHQTVKVVVDKVYDWKDYKNALEYLGAGHAHGKVVLKVEVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA85</accession>
    <name>A0A1E5RA85_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cytochrome c oxidase assembly protein COX19</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2838</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Required for the assembly of mitochondrial cytochrome c oxidase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the COX19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RA85"/>
    <dbReference type="FunCoup" id="A0A1E5RA85">
        <property type="interactions" value="297"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA85"/>
    <dbReference type="InParanoid" id="A0A1E5RA85"/>
    <dbReference type="OrthoDB" id="268594at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051383">
        <property type="entry name" value="COX19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21107">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21107:SF2">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ83797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="87" mass="10124" checksum="1C42575C90193D87" modified="2017-01-18" version="1">MSGNPGNRLQALSPTPPERGSFPLDHFGECKEYMENYLKCIRLTQGENAHNCRLLAKNYLNCRMEHELMEKDDWKNLGLPDDQRSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAD0</accession>
    <name>A0A1E5RAD0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Transaminated amino acid decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3103</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TPP enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAD0"/>
    <dbReference type="InParanoid" id="A0A1E5RAD0"/>
    <dbReference type="OrthoDB" id="308383at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004737">
        <property type="term" value="F:pyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000949">
        <property type="term" value="P:aromatic amino acid family catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02005">
        <property type="entry name" value="TPP_PDC_IPDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07038">
        <property type="entry name" value="TPP_PYR_PDC_IPDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000024">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012110">
        <property type="entry name" value="PDC/IPDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047214">
        <property type="entry name" value="TPP_PDC_IPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047213">
        <property type="entry name" value="TPP_PYR_PDC_IPDC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452:SF3">
        <property type="entry name" value="TRANSAMINATED AMINO ACID DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="11">
        <location>
            <begin position="28"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="10">
        <location>
            <begin position="527"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ83831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="696" mass="78994" checksum="63559E0466462F5F" modified="2017-01-18" version="1">MINKMVDDMSSSSSSSSSSSSTNYQKNNVGDYVFERLSQVTKTVFGVPGDFNLSLLEHMYNDTTDSNDSNDSNGNKSQFKTQVKXCGNNNELNAGYCADGYARINNGFGCLITTFGVGELSAMNAIAGAFTENVGVLHIVGMSPSFMHNDNHSNDDNDNIDNTNLFHSLHHLLPNKNTYKKQNHMVYTEMVDSLSCCKEILTLDDLTSMIDMDSDKTNYSNKTFASTCGKVTKLQAKIDNVIEQIIHQMKPGYLFIPCDLPDMDFEENLNKQLNINTNTTITTNPLYKPVSXVETEIVQYLLNETYSASNPGFISDYFMKNDKLTICQLLQKTNWYCYSTPGSRGFALDESLQNFQGVFCNESSTLNTLSIMDTNDFIMHWGANINEMNFKIRSNCNEKWLEGLNIVYLGKDYVQIGYNTTNGTSNFKLYTDNVNCSRIFNEYVKNLDFTKLSEIQTRSELNQLSNGKKVLLPQWSDSYNLDISSTTNNNITQQGFSKKLSTMGFLQKGDIIINELSSFLFEMENVKIPELSRTVNQMFYASIGYALPCTLGVCMALKDLEIADKHRVVLLQGDGSAQMTLQELGCFLTYDVCPTIFMFNNSGYTIEREIMGRFKSYNDILCNWDWCNIFKTFGDKKEEKHVARKITNYKELNEFHENVMNNQEFNNKIKMAELVLDQNDVCNSLKNFVTKQTKRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAN2</accession>
    <name>A0A1E5RAN2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP phosphoribosyltransferase</fullName>
            <ecNumber evidence="5">2.4.2.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g2747</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of the enzymatic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + ATP</text>
            <dbReference type="Rhea" id="RHEA:18473"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="2.4.2.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ATP phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.17" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAN2"/>
    <dbReference type="FunCoup" id="A0A1E5RAN2">
        <property type="interactions" value="207"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAN2"/>
    <dbReference type="InParanoid" id="A0A1E5RAN2"/>
    <dbReference type="OrthoDB" id="2574at2759"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003879">
        <property type="term" value="F:ATP phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd13592">
        <property type="entry name" value="PBP2_HisGL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.120:FF:000003">
        <property type="entry name" value="ATP phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000123">
        <property type="entry name" value="HIS1p ATP phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00079">
        <property type="entry name" value="HisG_Long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020621">
        <property type="entry name" value="ATP-PRT_HisG_long"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013820">
        <property type="entry name" value="ATP_PRibTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018198">
        <property type="entry name" value="ATP_PRibTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001348">
        <property type="entry name" value="ATP_PRibTrfase_HisG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013115">
        <property type="entry name" value="HisG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00070">
        <property type="entry name" value="hisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03455">
        <property type="entry name" value="HisG_C-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403:SF8">
        <property type="entry name" value="ATP PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403">
        <property type="entry name" value="ATP PHOSPHORIBOSYLTRANSFERASE ATP-PRTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01634">
        <property type="entry name" value="HisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08029">
        <property type="entry name" value="HisG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="GlnB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01316">
        <property type="entry name" value="ATP_P_PHORIBOSYLTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 17" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP phosphoribosyltransferase catalytic" evidence="15">
        <location>
            <begin position="58"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine biosynthesis HisG C-terminal" evidence="16">
        <location>
            <begin position="224"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ83952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="299" mass="32913" checksum="88FE36F006911026" modified="2017-01-18" version="1">MDLVNHLNDRLLFAVPKKGRLYEKVVAILKNCDISFRRSDRLDIALSTNMPIALIFLRAADIPTFVGMGKCDLGITGVDEVQESNQENVQLSLDLNFGKCKLQIQVPEKGEYKKPEQLIGKTIVTSFVHLSSQYFAKLENTTVDKMTTKILYAGGSVEASCALGVADGIVDLVESGETMRACGLTDIATILDTSAHLIESKTPQSNNLELIETIKSRINGVLTAQKYVSCYYNAPKENLDKLLVITPGRRAPTISDIGEEGWVAVSSMIERSQKGFIMDELKKNGASDIMVFEISNCRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAT3</accession>
    <name>A0A1E5RAT3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein BCH2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAT3">
        <property type="interactions" value="105"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAT3"/>
    <dbReference type="InParanoid" id="A0A1E5RAT3"/>
    <dbReference type="OrthoDB" id="434695at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034044">
        <property type="term" value="C:exomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015374">
        <property type="entry name" value="ChAPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31975">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31975:SF2">
        <property type="entry name" value="CHITIN BIOSYNTHESIS PROTEIN CHS6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09295">
        <property type="entry name" value="ChAPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="974" mass="113541" checksum="2B62E79899A4BD8A" modified="2017-01-18" version="1">MNYLNIFKTSSKSKIQSKQEDTSSSKNESSKQKQDATKAERLFKLSKGHNYSKSTLSFINSKKNNTKTVSGENDIDSYLYPNRTRNASAIFSTTTADQLSEDQKETADVLSTXNEGLDEDHLFHLNNASRKPQIDQITSPLVVEETVGECIQLRNKFFLYMFGDFPVSXEITDTSACEFLEVQQPILGPPDMIHVTTRNNKFTTDEIGQFFFVTGIPCWNFDLMITYMKFVFEQVSVPSCGKVKTEPSGKSKLSYQDFIQFQGRQNESKSGKKSAQRNNPTFKRNNKTKANXNVVTLFMYDVFRKSDVRLKYDIDDGSYQIYLFDPLDGHSDYSNGFADDNDKYGSDHVEEVAIWDKILISNIMRSIIFNKXSEWKIPGLVELDFLIKHGDKNDLVMTFEILGRMLEYMPMLLLDSGVEETLENEVNFLNNYMIQSFMELLNIFPTFYDAVLQRLKNDLLCDPEYADCYDLVYLFVLLRCNTHELEFVEQMYATLQKYKQNQWITSQLLFLQLEFLFRYQKDFKLALRVNFQLIKQKPYMIAPWVYLTLIYKETQQYEKLLHSMNNLPRDFVSDAISINNSIKAKYIKYFQTQYVEKYFQNGCFRNAQLDYLSRINNLSEQDVLSFSKGQVFYPLPCLSLNGYIDHIWNNKTILQMSPVLCGNKCHNLLDFANVKEIKILETTNEPLLEQSVADFATDLDKFVYTVLAEIATYLGWSSFAREFNSAFSNARFSSEMVFXESKTLEKKVSFDDEEIKDQRHCKXWFTAIVKNYCQDRKLVSXYFQELHSGKELVKSNLEWNLYGCLLYKTRNFRECVQCFRTCIENRFDPISAVHLLNICLFDGFDFDALGIVHSDQTISENQLEAQLSISVKTILRXLVHLISYDFRFYNNIQIRNIAYLKRXLQIFFNNDTELFINALYSLVYSPSQNMGEFKQENEADFKNKSVVRSXKNITDTHTASLINSISYVVHYYIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAT5</accession>
    <name>A0A1E5RAT5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2905</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAT5">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RAT5"/>
    <dbReference type="OrthoDB" id="5562676at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018786">
        <property type="entry name" value="Mit_KHE1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28062">
        <property type="entry name" value="K+-H+ EXCHANGE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28062:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10173">
        <property type="entry name" value="Mit_KHE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="2">
        <location>
            <position position="308"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="2">
        <location>
            <position position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="384" mass="44003" checksum="89CACB6BC8781DCD" modified="2017-01-18" version="1">MFRGRVCSILPKSPLLASKTANKNSQLILCAKRFNQSXSKASFSAPAETQVLENKNVNVLPKEKPSLPISESFDAFLNDPVKLIVIPITKDKIFLYYKHSDILLNSDSKIVKYETKLSQKAAQIWNKLETSPRSYNKLLVKWVNKFLNQVTWEEHSLASIPSENFVLKRINENTKIKDFFPDKSRISLEEYMRYSQMEKTKIVDDPEHKYALAKSVEPVKLYYPDSVLSPPGILSKINKLSKFGIAYHKKQMLYCVLALPLTFPLILIPLLPNVPGFYITYRFYCHLKAYLGAKHLKALCNNEHGSWLQFAKLQPYSDLFAAREGTVEKXHLETKDNNKTGVSFDLLTESQVDGVTXLLKIPEIKRNLXKXIKQEDKISKMANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB35</accession>
    <name>A0A1E5RB35_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein MSS51, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2950</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RB35"/>
    <dbReference type="FunCoup" id="A0A1E5RB35">
        <property type="interactions" value="183"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB35"/>
    <dbReference type="InParanoid" id="A0A1E5RB35"/>
    <dbReference type="OrthoDB" id="5282002at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046824">
        <property type="entry name" value="Mss51-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032717">
        <property type="entry name" value="Mss51_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28069">
        <property type="entry name" value="GH20023P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28069:SF1">
        <property type="entry name" value="PROTEIN MSS51, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20179">
        <property type="entry name" value="MSS51_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13824">
        <property type="entry name" value="zf-Mss51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mitochondrial splicing suppressor 51 zinc-finger" evidence="1">
        <location>
            <begin position="87"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Mitochondrial splicing suppressor 51-like C-terminal" evidence="2">
        <location>
            <begin position="232"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="441" mass="50921" checksum="352F8B6B3FBB385C" modified="2017-01-18" version="1">MIGYKTLAVSPKCAYASVSHSSTGVSNALCSQLTTKRNLMGFVRNALGLDPPANPHDPTPENRFHPWVESPYPDLQNRAKRIMEIARCPVTNEKINFTCPLSGIPTHASKEAWERDTKYHESKKYELLKKCNIYEHDLRSGRPFPEFDFPLAQDRDRMINVSNWDLYFYTRQYYSMDTEFQLAAVTKMLSYPITIASVLHQFSPYSLNPKGPITLEGLKSLSALRYTLYPGEQSKLGKNLAKVNNRAMRIFILGARAESQLPPHVWKQLNFLYPGQNFEIHFIGPECYYDRTKRKYITSNEPIKQVVDNSLTFVYHTDYFHVYHLQQDFFPYDPYLDVFFCFHPGFGAAESKSNWMGESFKALLETKCGVFTTGYHKADLLNDWTVLKEAGQGKMDVLMDPTKNVFGSTKWELNDANPQEVYQFNMWMAGFRGKRYHAINV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB43</accession>
    <name>A0A1E5RB43_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">MutS 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2935</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB43">
        <property type="interactions" value="168"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB43"/>
    <dbReference type="InParanoid" id="A0A1E5RB43"/>
    <dbReference type="OrthoDB" id="3973512at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051026">
        <property type="term" value="P:chiasma assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011184">
        <property type="entry name" value="DNA_mismatch_repair_Msh2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF20">
        <property type="entry name" value="MUTS PROTEIN HOMOLOG 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005813">
        <property type="entry name" value="MSH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="5">
        <location>
            <begin position="644"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="6">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ84116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="838" mass="94723" checksum="A93F1CDB78DE9FD5" modified="2017-01-18" version="1">MDESAELTINNNSFDISDGVMMCVDCIGTKMGCALLKMSAQCLYCLQQDLVFNSKTYNDEFXKTMHNLNIMFEPTVLYMSTRIDATIKKDMLDIVESQNMNLKIDTVSAAKFNRFAXAKAFREMDHTNSKYFTILXNALHSTGSMVTVGAISXLLEKCTSQMQILSNSDAQNAXDLFVSISYLPLNDFSFLLDEETIYSLKLLPSRKTQFQKSIDNGAYSLFEILGAFVTTEMGKCCLKNWILNPLKSLKKIKERSSFIETLLEEKTTNKASIYQLSKLLNKMPNAVHIVASIETGKAKYNTWVQLCKFLELTIDIVNYLRLCFPTKSVDRPYIIETVSKIELDNIKQLLNHITEIIDLPDSIEMRQIVIKRGVNKELDNCKHMYNNLEYVLSEVADKFNKAFASFNSDSALFNVVYVPQLGYLLCSASTNQNLVLNEHTKDTRLEVLFQTETHTYYKNETMDIMDANYGDIYTVVQDMEIEILQDLQNELIKNFKPXLVELCQSFSEFEVLVSWTLVSFERNYCKPEFSNEKEIVIKSGRNAINETLVASYIPNDLEINSTNTCGIITGPNMSGKTSYLVSCGLSVLLAHLGCFVPCSKMRLSVVDQILTRIWTRESVIKTQSTFYLDSQQMAKCLSICTSXSLVLIDEFGKGTNVLDGPALFGAVINFLTVAVKPCVLACTHYHELFGPGILGSQLFGVKFYCTQIILSTPTIKPAFEQXSVNQNELITFLYTVKEGISTDSYGILCAEHCGMKKKIVQRAKELSSVMNKGKNIVEYCSKISEEEIKTYEINEKIATRFLEWDLSMDASLSKEKLRAKLQXLLRRSSSAXSCDTTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBF1</accession>
    <name>A0A1E5RBF1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">tRNA threonylcarbamoyladenosine dehydratase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g2506</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the ATP-dependent dehydration of threonylcarbamoyladenosine at position 37 (t(6)A37) to form cyclic t(6)A37 (ct(6)A37) in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HesA/MoeB/ThiF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBF1">
        <property type="interactions" value="199"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBF1"/>
    <dbReference type="InParanoid" id="A0A1E5RBF1"/>
    <dbReference type="OrthoDB" id="10265862at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061503">
        <property type="term" value="F:tRNA threonylcarbamoyladenosine dehydratase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061504">
        <property type="term" value="P:cyclic threonylcarbamoyladenosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00755">
        <property type="entry name" value="YgdL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000125">
        <property type="entry name" value="tRNA threonylcarbamoyladenosine dehydratase 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43267">
        <property type="entry name" value="TRNA THREONYLCARBAMOYLADENOSINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43267:SF2">
        <property type="entry name" value="TRNA THREONYLCARBAMOYLADENOSINE DEHYDRATASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="12">
        <location>
            <begin position="68"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ84222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="448" mass="50650" checksum="7CF75FB81EC7C727" modified="2017-01-18" version="1">MSTDQNNKWRLIAGTAILAVAATKCIEKGYKLYKSGNNNTHYTKKDSFSSKKIANKKYDDSLFREQLARNYAFLGEQGMENLKKQYITVVGCGGVGSWVVTMLVRSGCHHIRVIDFDQVSLSSLNRHSCATLEDVGLPKVEVLKXHMSKIAPWCDIEVVNELWTLEHTERLLTFSEGNRKPDWVVDCIDNIDTKVDLLEYLYKNNINMISSMGAAIKSDPSRINIGDISTTEEDSLARACRRRLKKRGITEGIPVVFSSEKPDPNKAQLLPLPEEEFLKGKVGELSSLQDFRVRILPVLGTMPGVFGLTIVSWLLTKIAGYPMEPIVGKNRYKVHDSMHQSLAGQMTRIGMPDQRVPISTQDVPYILEEVFRGKSPISGFSTRLTLSKWDPSKPLSLQNVVIMTREEQKTHEEKVLNGNQSLLDVYSKDVLDLIAKRFAEEKFYSEFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBF4</accession>
    <name>A0A1E5RBF4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Lethal(2) giant larvae SRO7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3054</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat L(2)GL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBF4">
        <property type="interactions" value="157"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBF4"/>
    <dbReference type="InParanoid" id="A0A1E5RBF4"/>
    <dbReference type="OrthoDB" id="19944at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045159">
        <property type="term" value="F:myosin II binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013905">
        <property type="entry name" value="Lgl_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241">
        <property type="entry name" value="LETHAL 2 GIANT LARVAE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241:SF25">
        <property type="entry name" value="TOMOSYN, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08596">
        <property type="entry name" value="Lgl_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lethal giant larvae (Lgl)-like C-terminal" evidence="4">
        <location>
            <begin position="542"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ84227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1026" mass="112230" checksum="9AEE0F8E186CB0B1" modified="2017-01-18" version="1">MFKSLRLKKKKDSAPKSSTPPPPSSSPNTTTKSSSKFSGKTSSITPKIYPGSSNLSKRFVLHEVSRYGITGQITAMAFDHTQSLLAVATSSNEIHVYGQQQIEVQFVLSKLDAYMVHLCFVKGIYLVCVDAKDTVSVFSLYSKELLATFFAPNKITCVESDPTMDWLLIGLQNGITMIYDIDRDGVSPVEIQNLQKEQFFSGHGISPVVSIQWNPRNIGQILISYERVTLLYSFIEKSYKQHFVYELPIGAPGGDGKVTSTVRYPKVVQSLFHPNGLHILTVHEDNSLCFWDCNSGKLILARSLYETGVNIPTGMTTPQPANLSASIVSVHWICAENPEYTQLLIAGGDLGSDKPISLTLIDLGGTPLYSVTSYEKMAKFYSIRSNQRIVPMPQHRSVAKVLPVPRASPFYNGCHNPGIVLILLDNGEIETMLFPSGKITYKASLLPQSLSWMRPSATISKGASVPSKLWVGMMTSTYNKDSIXKGGKSRKKQIRPYATRSALLTGHSNGSVRIWDASHAELDETSVFDINLSQTLNKADANITHISYAADNSELAVSCEGGEIVLFKFDVNKYYDPTGAKQMTARFNRFSVAATNSLLVDVSDRAPSSLKDGFVPSTAIHGNSGNVTALTNSNVGFVSAGFMNGALLVVDRRGPAAIFFENINRYTRNSAYVTSCEFGICAYEGDSYSSILMVCGTDSGEVLTFKIVPGVSGRFSVDFCTTMQALDSLAVENINFISNERNTSLRASINLMKELPTGTVLPGSIIVSGGNEVATFKPGSSKRNRIKPFKSVIASANISYIAYSSKDNQRKTALVYMLLSMTGDIKIYLAADMKEIKEMHLGFPVQSKYIKHSSVLENGDIILRVSASEAKLYSTVEDSAMRDDASKTDTLYMQNVSIPYRPYYNSLQRARGDAYVYKETLDKILGGNDDRPPSKYPESAISNXTLTVKPADDLANKHSYSAPTRRGTKSGYSYSRALYKSIENGYDSIESTVNDVATVAGQTMTESLEASGTELAKGLFKSGFGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBP1</accession>
    <name>A0A1E5RBP1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YCII-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2694</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBP1"/>
    <dbReference type="InParanoid" id="A0A1E5RBP1"/>
    <dbReference type="OrthoDB" id="5519740at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1060">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051807">
        <property type="entry name" value="Sec-metab_biosynth-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005545">
        <property type="entry name" value="YCII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33606">
        <property type="entry name" value="PROTEIN YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33606:SF3">
        <property type="entry name" value="PROTEIN YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03795">
        <property type="entry name" value="YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YCII-related" evidence="1">
        <location>
            <begin position="4"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="103" mass="11337" checksum="92A0196B83C732F6" modified="2017-01-18" version="1">MVEWIAVVYDKPNVDRLKFRTEHLQGIPPAFESGELTSAGAIFKGLNGEGKPEGFAGSVISVNADSREDVIAFLKKDPYHVNGIWDTENALIYPYGVAKRNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBT1</accession>
    <name>A0A1E5RBT1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative metabolite transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2667</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBT1">
        <property type="interactions" value="1157"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBT1"/>
    <dbReference type="InParanoid" id="A0A1E5RBT1"/>
    <dbReference type="OrthoDB" id="5290825at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005366">
        <property type="term" value="F:myo-inositol:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904679">
        <property type="term" value="P:myo-inositol import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF12">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="63"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="225"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="387"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="421"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="452"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="483"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="521"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="554"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="65"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ84352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="602" mass="67101" checksum="AD68D37CFB66353B" modified="2017-01-18" version="1">MDLLEDPNGNSLYNTDTTNENFTLNNASCNYFDDRYSMLSLLSQATGSDLDDQTSFKWTFRTMLIFVNGTVASLLFGYDTAIVAGLLLAIEPEDINCRELSDWNKELITGITCIGCLIGASFYIADKYGRKFVLQLAMIGFVVSSIVMALAQTLLILVIGRFLIGISIGFASTIVPLYLSEISPASIRSSIMTLHTVSITGGQVLAYIISMIFHKYYSANSYKTFAAWRYLLILPVIPALLMLIGLPLVPESPRHLIYNGELKEAQKALSLIYPLASKDQINRKIIKTVKDIIKIGSPKNNENEHFESVPLLTQVNASSPTSSAGLLNSSMTQSGSILRRPSEGNLNQFFQEIMANIKKTHNNARXKKHQKLWFHNFQKMDPRTKRALWVGCTLMFFQQICGFNAFMNYSPLIFKPYSSNPLIAGFCVSLTNFLGTLVSLKFINKYGSRKLLIYTIPGMVVGLFLCVVAFAEAAGANSINPDHIAQMLLISMLIFVFAYASAMGNVPHMSVAFLPLNKKSFGTSCILMVNWSTNFLVTSTYLSLVSLLGDTTILSFYLSMTVLAWFFVWKCFPEIKGLSLEEIGEIFTDGIDVDYVYKQYHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBT6</accession>
    <name>A0A1E5RBT6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulatory protein DOT6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2975</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBT6">
        <property type="interactions" value="209"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBT6"/>
    <dbReference type="InParanoid" id="A0A1E5RBT6"/>
    <dbReference type="OrthoDB" id="3971938at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="88"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="88"/>
            <end position="138"/>
        </location>
    </feature>
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        <location>
            <begin position="16"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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        <location>
            <begin position="419"/>
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        </location>
    </feature>
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        <location>
            <begin position="492"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="615"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="716"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="425"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="436"/>
            <end position="447"/>
        </location>
    </feature>
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            <begin position="452"/>
            <end position="463"/>
        </location>
    </feature>
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            <begin position="512"/>
            <end position="524"/>
        </location>
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            <end position="699"/>
        </location>
    </feature>
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    </feature>
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            <begin position="749"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="775"/>
            <end position="791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ84364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="824" mass="90393" checksum="BA493B37024A4985" modified="2017-01-18" version="1">MTFYAQSSLVMPVQASHHALHSDHGNTMASSGIHXSNNSNSKSKIEKNNNVASSHSGMSSLEDFSQDASDVSKNSSDSTPYSTQQEDDSSKIPSSWDPQDDILLRHLKEIKKLGWKDIAQYFKNRTPNACQFRWRRLKSGALKHKSVASTMELTDHQLENVIIPDLPESNGAVSTTNTSSYNVXENDLKKHSRVRKSRASSMISSSTPRIDESMKTNDRTHLQKAQQQNXEHQPVVAPQKGFFSTSTAPAKDMNGKFNAMSQVTXGFAKPRTYSSSNTASNFSKNPIYYEQNQQAYLGFQSADMNNHVQQQNNGIHGNAPTNGIYTSTHTSQLPLPSQSQHAVSKNDEKIGFIPKVVVRSRRGSSVSNVNSSSSGTTGMLAHGNGFYHADSYSSAPAMKNSFSVSSGFPTNINPATTNAPQSYVSSSSSSSSSSSNPNXGSXTFSTPKSRKNSFSSRSRASSFHTGPNERRLSVLLTEKELCIDEKKLENKSRRSSVSVLNRRRGSFIKPHQPSSHLPSQQMHLSSSISSNNASNTSSGHGHHAVPWSCEEDRILLNXSLQKDEISILLGNRSDLEIQKRVXELQHIGSAQQKRKQGHQSSCFEDAPRHVNEELAISDSTSSSSKNVTPSPPIFSEQDREGGVSSVVSSTHSTSTHSTSNHTNQSRFSPPYPQASGSFNNRPSMASNMITPTTSGSGQRLKQRDGRLDAQRVHLNATFPTQTHNVKPTSNAQSASHFGATKNGNSPKDSDEDAILDDESEEAASSMNTNPASFNQQQQQQQSHPQYQPQQSNSGMYNHFQQHHAEHHQPQQSLPSLNSILRDMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RCR7</accession>
    <name>A0A1E5RCR7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">5-demethoxyubiquinone hydroxylase, mitochondrial</fullName>
            <shortName evidence="8">DMQ hydroxylase</shortName>
            <ecNumber evidence="8">1.14.99.60</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Ubiquinone biosynthesis monooxygenase COQ7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">COQ7</name>
        <name evidence="9" type="ORF">AWRI3579_g2687</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the hydroxylation of 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) during ubiquinone biosynthesis. Has also a structural role in the COQ enzyme complex, stabilizing other COQ polypeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 5-methoxy-2-methyl-3-(all-trans-polyprenyl)benzene-1,4-diol + AH2 + O2 = a 3-demethylubiquinol + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:50908"/>
            <dbReference type="Rhea" id="RHEA-COMP:10859"/>
            <dbReference type="Rhea" id="RHEA-COMP:10914"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:84167"/>
            <dbReference type="ChEBI" id="CHEBI:84422"/>
            <dbReference type="EC" id="1.14.99.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="8">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion inner membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the COQ7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.60" evidence="8"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RCR7"/>
    <dbReference type="FunCoup" id="A0A1E5RCR7">
        <property type="interactions" value="363"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RCR7"/>
    <dbReference type="InParanoid" id="A0A1E5RCR7"/>
    <dbReference type="OrthoDB" id="275371at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008682">
        <property type="term" value="F:3-demethoxyubiquinol 3-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01042">
        <property type="entry name" value="DMQH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01658">
        <property type="entry name" value="COQ7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011566">
        <property type="entry name" value="Ubq_synth_Coq7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11237:SF4">
        <property type="entry name" value="5-DEMETHOXYUBIQUINONE HYDROXYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11237">
        <property type="entry name" value="COENZYME Q10 BIOSYNTHESIS PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03232">
        <property type="entry name" value="COQ7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0830">Ubiquinone</keyword>
    <keyword evidence="2 8" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ84373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="244" mass="27278" checksum="C6445DAFC84C7AB4" modified="2017-01-18" version="1">MFQRSTKVSYRSFSVLAPLKTLPTKPLNDGSVANTTSAIKLKQFKPLSANQKAYLDRVIRVDQAGELGADYIYAGQYFVLAHKYPHLKPVLKHMWDQEVHHHKTFDALQVKHNVRPSLITPLWKIGAFAMGAGTALISPQAAMACTEAVETVIGGHYNDQLRALHKNFDLQLLRSTETAGSVASTAKSEEIEELINTIRMFRDDELEHLDTAIKYDSHAAVPYMLLTEGIKTICRVAVFTAERI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDM1</accession>
    <name>A0A1E5RDM1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein MUM3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2086</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDM1">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDM1"/>
    <dbReference type="InParanoid" id="A0A1E5RDM1"/>
    <dbReference type="OrthoDB" id="189226at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036149">
        <property type="term" value="P:phosphatidylinositol acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983:SF70">
        <property type="entry name" value="PROTEIN MUM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="487" mass="57542" checksum="EC60DF4E3778AF50" modified="2017-01-18" version="1">MGAWKTLETLVTGPLINYWCVYDKKTLIQICYVVATALMFLYAYYTVELYVTLLNXFTNAQEAHCGYKIMYCNIFIVKRLPFIGRSCGKKAEEKMEYIQKDVVPYFEKLEIFITTLFWKFLSDLCFKTNNKSAVLVITGDELNIHDKSISEYFTNDQDIPGGNTLIISNHRSLFDYVLINHVIMQSKTQDSIRQIYFQIFHKNSPTVNETLFITWKKILKVPSLKILXSILRNHENTGISKKDLLQHFQQSHTRGLHDNLRKTFNYVLFPEVSIITHELKLVQDKLNSMYGIPIFSNLLYPRFRSFTQMIEYFNVLLGLKPKKENNLLVKKEQEILLEYQHEKSTQMFDADNLEIPMLQKHFFDFTITYYKPLIIDNQESLALHQHTVFHNNSHTNNEKSHQIPSCNYELMHITPSLFEMLVLNDRLQNADKMPIIIRLHIQKKDLKQLLSLNEKKLQKWLEDDWKEKDQIIDLFESNIKLVKTKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDQ9</accession>
    <name>A0A1E5RDQ9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Mitogen-activated protein kinase KSS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2342</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDQ9"/>
    <dbReference type="FunCoup" id="A0A1E5RDQ9">
        <property type="interactions" value="699"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDQ9"/>
    <dbReference type="InParanoid" id="A0A1E5RDQ9"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000040">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="20"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="389" mass="45039" checksum="7571001D3BF71C16" modified="2017-01-18" version="1">MSNQSTVKTKKIKFNIPPYYQLKELIGEGAYGIVVSAVHNYLIPETNEQILTNVAIKKILPMGKPLLLTRTLRELKLLKLFNDHENIITVLDIVKPNTYESFNEIYLVQELMDTDLSKIINSARSSNNPVEITNDHMQYFTYQILRALKAIHSARVIHRDLKPSNILLNSNCDLKICDFGLSRCLLSSSSSKESLVGFMTEYVATRWYRAPEIMLSFQEYSTAMDIWSCGCILGEMLNNGKPLFPGRDYHHQLWLILDVLGTPSEEDYDTIKSTRAKSYVKSLPSTQRKNWADLLPPGRDPLAIDLLEKLLTFNPYKRISAKEALKHPYLQLYHDPQDEPEYQPIDVLEDRFWKLDSDINFQSWEENEENSELMELLKKLLYEEVVKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDX6</accession>
    <name>A0A1E5RDX6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein EMP47</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1971</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDX6"/>
    <dbReference type="FunCoup" id="A0A1E5RDX6">
        <property type="interactions" value="156"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDX6"/>
    <dbReference type="InParanoid" id="A0A1E5RDX6"/>
    <dbReference type="OrthoDB" id="10265193at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005537">
        <property type="term" value="F:D-mannose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051136">
        <property type="entry name" value="Intracellular_Lectin-GPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005052">
        <property type="entry name" value="Lectin_leg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223:SF28">
        <property type="entry name" value="LECTIN, MANNOSE BINDING 1 LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223">
        <property type="entry name" value="VESICULAR MANNOSE-BINDING LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03388">
        <property type="entry name" value="Lectin_leg-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51328">
        <property type="entry name" value="L_LECTIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184705" evidence="7">
        <location>
            <begin position="26"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="427"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="L-type lectin-like" evidence="8">
        <location>
            <begin position="39"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="462" mass="51911" checksum="E6A2CC0AD8CDFE7F" modified="2017-01-18" version="1">MYTRFSLSFAFLLFLGITQFFTVEAHSSASKGSSSGHSDAELLNSVMSLPDLIKAKKNPPSYDFFQSATLEEGRIVLTPEPSTSGSIWTKSSMAMPDAFTIEWVFRSVDFQGKTDGGLALWVTDSQVTNPYLTSENVADFKFNGLMLYIDNNGPSGSSQIRGIVNDNNAKQFSTMQEFYDNEFGSCLLGGYQDSSVPITARLTYDNSGDTNFIKLQIDNRVCFQTRQISLAKGLNKKNLKIGASAQNGQNNEESFEILKLHYYEGVVQDSLIPNIRQMQQPKYVTKVVNQDTGDVTFVERKPGMLGDSELTLSAIYEKLNKIEGKVLANDVGIIYQEIENLVTINKQQSKRIEALINALSSSTLASTSSSNDKNSASQSGNLDMDNFKDFIKMDEKLEKLLAEQEKLREMTKLEKQTKLESHHFDDLFYKLLLWISPLVIVMMVMAYYTFRIKQEIVKTKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE38</accession>
    <name>A0A1E5RE38_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Swiss Army Knife RNA repair protein HAD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1687</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RE38"/>
    <dbReference type="InParanoid" id="A0A1E5RE38"/>
    <dbReference type="OrthoDB" id="5596992at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990259">
        <property type="term" value="F:histone H2AQ104 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008649">
        <property type="term" value="F:rRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018812">
        <property type="entry name" value="SAK_HAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335:SF23">
        <property type="entry name" value="OB FOLD-CONTAINING PROTEIN, NUCLEIC ACID BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335">
        <property type="entry name" value="RRNA 2-O-METHYLTRANSFERASE FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10307">
        <property type="entry name" value="HAD_SAK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Swiss Army Knife RNA repair protein HAD" evidence="1">
        <location>
            <begin position="70"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="536" mass="62750" checksum="CEE0C41B593D7D61" modified="2017-01-18" version="1">MHKRRKLQTLKGKPEDQETKSFLQINLTMSPAEVIEKYHSLPNVEPKTLVDPKKIKTINIFDFDNTLFNSPAPNPHIIEPYSLNVLSYPYHLHTGGWWCQPSYLNALVDEWLEDRRHLEKSGIECSVKHNYQDEQCQCDECKIDRKYWNPHTCRLLHESSLQEDNTLTLIMTGRRNDRFAPVMEKVFANTIFKRRIHVNGVFLKDPTFSSTMAYKTQVLSDLLLTFKPQLLVMYDDRPSQIKGFKSFLYDFRRSNESKLPYFRYEVVLVPRLVRYLSAYKERLLLEQEIKEHNEISQKQALHDRLNNSNGSSSLDDEAGKLHEIQFDQLQIVDKNIKAYHFLQPLYKKKLVSKTKELLQEKFPESELSFNENELVFEPIGIRLHNESREEFSKHIMLLDAYTTKHDVPNPSKLQLLTDSSNAFYTTGYEEPIEWTLTKLNVAKDMNYIYYTCEPVTPNLATELQPAVPGLIVAYKKKYVESLNNNDIMLSEEDTAKKENYTVIDLNSSDLSNTIITHFGYKFEIHVDETPEKKSVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE62</accession>
    <name>A0A1E5RE62_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin-protein ligase E3A N-terminal zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2162</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE62"/>
    <dbReference type="InParanoid" id="A0A1E5RE62"/>
    <dbReference type="OrthoDB" id="193703at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.50">
        <property type="entry name" value="Ubiquitin fusion degradation protein UFD1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.130.10">
        <property type="entry name" value="Ubiquitin-protein ligase E3A, N-terminal zinc-binding domain (AZUL)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032353">
        <property type="entry name" value="AZUL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042556">
        <property type="entry name" value="AZUL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004854">
        <property type="entry name" value="Ufd1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042299">
        <property type="entry name" value="Ufd1-like_Nn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12555">
        <property type="entry name" value="UBIQUITIN FUSION DEGRADATON PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12555:SF13">
        <property type="entry name" value="UBIQUITIN RECOGNITION FACTOR IN ER-ASSOCIATED DEGRADATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16558">
        <property type="entry name" value="AZUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-protein ligase E3A N-terminal zinc-binding" evidence="1">
        <location>
            <begin position="615"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="730" mass="83325" checksum="D5F56A9297253355" modified="2017-01-18" version="1">MFGTITVDKSLHPLSDKVVLPEAYFHELLNNKQLEFDTSKPAILELQVVTADGNLHSKCFCGVREFSLQDPDEIQLPYAVFLNLCLNTLESNNFRLDTQLVSQEIPGGTEISLEPLGEVQWVNXMENPTAYLAEEYKVDAFNKQFIDNDTFVKHFLEARFNNALTVLSTTNSILFVSNSENGARSETIYKFKIHDLKPSSAICIVNVDLSLNIVHQVQIPVDKENRQIGPARPLNASNENHISDPXPISKIQPNEVITVLDPQXIYQIXLEKXHVFQIVANDINESFQXFVGSDEKVNWDCFEYSSVGSSCTKTTGHTCSGATFDLPACSQPYFIKASLIRNTPEQVSSEFQFIVKPKEXNASSDSDILSLDVPPGFXLCPHCNKTISESAFLMHELRCQKNITKCSQCQEVFETVSRDHLTXNHWHCNELHEFFIAGTQFSVAECHGESQVSHEEHFKWLHDLESNSQNCDWCLEFKTNSLYALGLHKEYECPEHPKMCRFCNLVVSRGEKNFETQYFGLTGLHEFQCGSKTVDCYKCGSKVRQFEMEQHLLLHKMQLKNQVLNSTLSVCSNKNCCNVTENEDFNNRYRLCKFCYSKFYSTDYDKDGKRFQNRLERSYVLQLTKGCGYTHCKNIMCRSSGMAKSFQNIKEVLMFIQDRLLALISKPEFYLCVDDSITKKKLAADIYLDNMNSNGYTQSWVYKAVNSTKSSSLETLNDWLHENALGKNDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REB8</accession>
    <name>A0A1E5REB8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Trehalose synthase complex regulatory subunit TSL1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1693</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REB8">
        <property type="interactions" value="312"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REB8"/>
    <dbReference type="InParanoid" id="A0A1E5REB8"/>
    <dbReference type="OrthoDB" id="755951at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005946">
        <property type="term" value="C:alpha,alpha-trehalose-phosphate synthase complex (UDP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000099">
        <property type="entry name" value="Alpha,alpha-trehalose phosphate synthase subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF15">
        <property type="entry name" value="TREHALOSE SYNTHASE COMPLEX REGULATORY SUBUNIT TPS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="38"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="212"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="320"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="359"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="38"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="105"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="212"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="321"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="421"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1374" mass="150926" checksum="9FA3CC3D20F8ADA1" modified="2017-01-18" version="1">MVVLVASLFLPFKPQFEIDASMNEAAALLESQNIKIPAANPSTNTSSNTNPHAANAGNAESESVSAASPELITRNRTNSSSLLKKYGSATNSRSPSVIPLDRDTTPANTAATNATDSTGNDEXSNNVSKGEGTEASNTNNNHIKNRSRSKSQLLQRTNQLETDTKAASDAFMESLTANATNSAVETPRPSNTIAGMSNTSLASVAHGQQSLNPTAAAAAAANAGPQQQQQLHQPVPNHHHPLSKMHAGGGNAEFSFDDSSSDIATSSLNNNTNYSSAGIQNDGGTPVSLMQLQLGGGLTTHSATPTGLGSSSSYQHLAPLSAQTQSQTSQAAQNPHHHHHHQPDRLDPTAALLKNVSKNLAGSSSGGGMPFPSSSPSSATLAGNKFVPPRPSHLSTVITPKSRDLPASEQSALNSGKYRDMSSMPSTTSMRRVHRAXSGATPLQATTATTGSSLHLDNNQKAGIKEEKLEGGDADTNNASLKAGASTGAARKKFTQHHDYSLESLDHEVFFSDDELPANAIGDREGNDQLYLDNEVPEFGGYSNATNLVKTSLLGGSQNIFSKATWEIVASHKCNTGLKNAVNTAILEKTFNQEIVWVGSIGIPTDALPEDVLGNVTTALDNDFHCKTVVADDITFKGAYKNFCKEILWPTFHYQIPDNPKSKAFEDHSWNHFQRLNQLYADKIVESYQKGDTIWVHDYHLLLVPGMIRAKLPHAKIGFSLHVSFPSSEVFRCFAQRKHILRGLLGANTVGFQTEEYARHFILTCNRLLMTDGDIEDGIKYDGRVIEINTTPIGIDAFNLQTQLQNDSVTACRQLIRDRWGKDKFIIVSKDQFDSLRGLKNKFLAYERFLKTHPHYIKKCCFIQVFNGSAIKDLDLERDIVNIVDRINSLSEDISLSTQNVVFLHQQLEQSQYLALLSEADMFLVNSMREGMNLTCHDFIVCTQEKNSPLLISEFTGSAELLKRGCLLINPWDIRRVSEMIKFALEMGAEEKRRKWKRMMKQIIINDSDNWIISMIKAINASWELNKQSSTLFKLNYPEVYSSYTSCKTRNKLFILKISQPPNGEVLKMLNDLCVHNEVYVLNSFDKATIERYYARVNNLNLIAENGAFVRIHGYWYTMMNDCSWTREVLPLLQSKLERLPGSYIKSGESMFRVHTENAEDPERVNDVVGDLILHINTLFGSRKGIHAYFHKNIVFVQQYGLSLKALKFIFSYYASHAGELHSVTSNASNGLMSPMKTPSLQRQGSSQSGGYFMSRGDSNNSLSSLNADDLKLRPKMSPTPSFDNIATLNSSQVGMVVDDKRGVNKFSFACITGSSSPVIEPLFKYINSLKRDVSYTFTCVYGNPSSTNAKQHVDGCNDLVNILSKLCAAVAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RED9</accession>
    <name>A0A1E5RED9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2301</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RED9"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ85260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="269" mass="31271" checksum="4CB616FF62D2459E" modified="2017-01-18" version="1">MFTNNLYYLSHKFRYENYPLKKSVVLDTHIASKTSCTHYSDDIHNGKNVATDXRSTYGESQILSDQEKKIDANVLINTYYFILKGIRYNMVHCNVNNCGDAVITDLTRSTNDGTTSTTTNNNITTISNDCNSNLNAYEYKGKEDDLICKQIMKNLLNMQYEKFKLHNSPRNGSSGKATANEIXSPTFYNKDSKLLQNQMKYWNILYRDYSYLQIKCNYTTNLLNKEHKTKDNKNSKKDNKADQFALKMLNISKFERDLVYLNEMLHTQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REF0</accession>
    <name>A0A1E5REF0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1869</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5REF0"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="203" mass="22086" checksum="E8F8BD0E41705892" modified="2017-01-18" version="1">MQSNQHSEQQGHNLGQKRTEQQKNQGLNTEQFESGFTSQNPSGYSATRTTKTTTYGDEPMEGNQQWGSSGDDNTGFGSNTGRTSGTSGGATRSSKMGGGGYDELEEAGDDDEFGKYSSGREHKXQGSSQYQEGTXGMHPDSAHADRHQRGAFQGNTASNDADQYGRSYNDDDDHTSGNRDKTEKKKNIVQKGADKAKDMLERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REG8</accession>
    <name>A0A1E5REG8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ataxin-10 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Copper transport protein 86</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1713</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play a role in the regulation of cytokinesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ataxin-10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REG8">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5REG8"/>
    <dbReference type="OrthoDB" id="379794at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051374">
        <property type="entry name" value="Ataxin-10/CTR86_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019156">
        <property type="entry name" value="Ataxin-10_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13255">
        <property type="entry name" value="ATAXIN-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13255:SF0">
        <property type="entry name" value="ATAXIN-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09759">
        <property type="entry name" value="Atx10homo_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ataxin-10" evidence="7">
        <location>
            <begin position="440"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ85301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="542" mass="63057" checksum="9499713BC2A978A3" modified="2017-01-18" version="1">MSIVDLEETYMQGICQCFQKNLDTKSRERLLVSALTTLNELVQNTSKNADYRLAVSTKTRWEGYLEQMLNLVSTTDFHTLFSSNRTLELDSVDLEMADLRTLYLRIFRGYIMLCRNLIVVSGSTVNFESKDYQDLHLKVLLSFXSIYKCSDFPXSSKRELYITMTEFLCNLTTIGNYTLDHTLFFEPCVQFLSFAKIYTESYVPWCIYLTNLCSKNQDFLYYSFKNELFNKDIVVGILLGSPILKFDNRTWDTTNMTTLQTLCIRLWYVLITHESFPNFFYALETRDYENFEEILEISTVIVSSKEAWDNFXLTNVMIWVXKIFDELIGYIDQYFQQPSNSRDAAEIEKHVEQCYNKLLSVLDIMSSLCKYEHIRKFMHFYKGLEKLIKLLGILEKNCVKLNLVKSSDGSXVIVGVKDNQGLDRVDLLQNKIKANNFPHCKSLIIEIISYLVYGNKEVQDKVRELCGLEIILSNCIIDDSEPFIKERSIVCLRYLLTENKENQDFVAKLEAQKPVQDSALDEAGFELKIDDEGKVGLKKRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REJ5</accession>
    <name>A0A1E5REJ5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2291</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REJ5">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REJ5"/>
    <dbReference type="InParanoid" id="A0A1E5REJ5"/>
    <dbReference type="OrthoDB" id="3995714at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="413"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="492"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="497"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="599" mass="67899" checksum="9ABFAD6F44A9FF70" modified="2017-01-18" version="1">MDFRNKKQPTSKLHNAHNINGLDEKAENPTLRIAVLGGKGTGKSSFISRLTVNLVPEVHYPTLKQNNWLLQFVPEKSRISKCLLDEHWHERMGHDIHNVNLVDGCIYPSPNLSQHLLLSKNLFQNATNDFIRFRQNYVTTSLGSTSLSAQTSSSSMKGNSLGNNKYXNTLPESVYAELNILKDKQENIYYKYVTGKVALAENNAAKHNYYLYQYDNSDSTNAYNSNHEFAFEVDDDDDDDDGNFYADGDKIRVTEKDLVELKKTSTHPLSSISTEDEKFTQRNNSNNTLSAIQSQGNPTFYVAGHDTLTVLKTIKEQNQMKLPLMYKPPAISPMLIDIIDTPPFDPEMVVPFLEVSLFRNIGSDYLHNLAKDPRRPVSTRSMLTASGANELNGNVDCYILMYSCYPETIEPPQYDDLEDSINGNGLTGKPXTRRPSSTNENDDQIMLLENIRESIEEAWEEFYNYKQNWEEGQEGDVYSVMYNLKKLWSSKPKKTTRSSSTTAKNSENGKNSNSKPPKKKKFRSFDDPLAPPPFIIVATHTACELTSPVLLQRGKDLATEWGCSFVAIDNYVDYNVEEALSLAIREVVERKKLNKSKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REN8</accession>
    <name>A0A1E5REN8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">5'-deoxynucleotidase</fullName>
            <ecNumber evidence="8">3.1.3.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g2045</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the dephosphorylation of the nucleoside 5'-monophosphates deoxyadenosine monophosphate (dAMP), deoxycytidine monophosphate (dCMP), deoxyguanosine monophosphate (dGMP) and deoxythymidine monophosphate (dTMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-phosphate + H2O = a 2'-deoxyribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:36167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18274"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:65317"/>
            <dbReference type="EC" id="3.1.3.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the HDDC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.89" evidence="8"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REN8"/>
    <dbReference type="FunCoup" id="A0A1E5REN8">
        <property type="interactions" value="509"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REN8"/>
    <dbReference type="InParanoid" id="A0A1E5REN8"/>
    <dbReference type="OrthoDB" id="10254258at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002953">
        <property type="term" value="F:5'-deoxynucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009159">
        <property type="term" value="P:deoxyribonucleoside monophosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3210.10:FF:000011">
        <property type="entry name" value="HD domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039356">
        <property type="entry name" value="YfbR/HDDC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845:SF13">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13023">
        <property type="entry name" value="HD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0170">Cobalt</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="13">
        <location>
            <begin position="60"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ85023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="219" mass="25350" checksum="707C5EB313EE6AC6" modified="2017-01-18" version="1">MTTTTWKPEDHIPEEIQESFKNLSSSPSAYVISLLSVVEQLKIQRRTGWVDHNVSPCESISDHMYRMGIAAMLLKTPGVDKAKCTQIALVHDIAESLVGDITPFDPIDKQEKHRRELATIEYLCEKLIKPYNEVAAKEIHENWLAYENISSIEARFVKDLDKFEMLCQCFEYEKRSNGTVDFTSFWSAAASIKTEEVTEWKNELYRQREAFFKSLEESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RES9</accession>
    <name>A0A1E5RES9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Vacuolar transporter chaperone 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2422</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RES9">
        <property type="interactions" value="99"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RES9"/>
    <dbReference type="InParanoid" id="A0A1E5RES9"/>
    <dbReference type="OrthoDB" id="6493944at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033254">
        <property type="term" value="C:vacuolar transporter chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006799">
        <property type="term" value="P:polyphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14480">
        <property type="entry name" value="SPX_VTC2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.100.30">
        <property type="entry name" value="VTC, catalytic tunnel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051572">
        <property type="entry name" value="VTC_Complex_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018966">
        <property type="entry name" value="VTC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042267">
        <property type="entry name" value="VTC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140:SF2">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE 3 COMPLEX SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09359">
        <property type="entry name" value="VTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="809"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="838"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="876"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="9">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="271"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="387"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="677"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="75"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="277"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="309"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="393"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="690"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="704"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="731"/>
            <end position="747"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="958" mass="108930" checksum="EDCFEBAE265974FF" modified="2017-01-18" version="1">MLFGVKLASEIYPAWKDNYIQYDQLKSLLKEEETKSNSGESTSKNNNKKNKKSTWTNNDEIRFVEVLDQNLEKVYSFQLNKYNEILNXLNKLEDEIDSNDLRDIDFKEFQTVLENSLQESQELDNFTRLNFTGFTKIVKKHDKLHPNYPSVKSLLLVRLKELPFHSEEYSPLLYKISFLYNIFRMNNKSGSVGGGTTANTNASSLSQSAKLNSVSNFTDVQFDTFKFWIHPDNLIEVKTRILRHLPVLVYASLPNENDDLIDRLESSIIDDTTSSTQPDSNVSAQGQVSGAQDPSGLIENVMDNENRPATAGSSTTTTTSGSDPQGNNNKSQVAFDPVITCVYFDNDILELYNKKLLKIKDYSSFRLRWTGRLTNKPDIFMEKKTFMNHGDQSENANSSTNPNSMGRNSGSKKTDDFEETRLKIKQKFINGFIHGSNEYKEKLINKLTKSGNDKDVVPVSQNCDDLQKFITSNDLEPVLRTMYVRTAFQIPGDDRIRITIDSDILFIREDSFDQDRPIRDPQSWHRTDLDSSVANPLKFLRTGEYNKFPYSVMEIKVKRPSSSTHNSFSSPASYSARHSKWISDLTNSHLVKEVPNFSKFIQGIASFYSEDDKLDYLPFWLPDVDSGIKIDPQQAFDEEQLKLQKQKALEKKQKKFSKRFSIVGTNDADFDFIKLKKDSNSTNEPNTAVEDDHIGSHKNKPQEEDVEEITGDIDEESSEDDFSETDSSLNKKSKKSKKSKKSKKAKNLKRDPNFFKILTDPKLTNVDSEDEEIELPPGVKKPTSYLKNAGPVKVEAKVWLANERTFNRWLHVTTLLSVLTFSIYNSTKKAAFPRLATYLAYVYFALSVLSGLWAYSTYMKRVHTIEQRSGKHLDAPLGPLVLATVISLTLIINFVAAFKSSYSQLHHPPQLHNPSNSTASAIIDFAVDNMGNGVHDSIELPHALKPIQDFIFKLVGAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REX0</accession>
    <name>A0A1E5REX0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ubiquitin ligase-binding protein BUL2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2416</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REX0"/>
    <dbReference type="InParanoid" id="A0A1E5REX0"/>
    <dbReference type="OrthoDB" id="2283785at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039634">
        <property type="entry name" value="Bul1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022794">
        <property type="entry name" value="Bul1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007519">
        <property type="entry name" value="Bul1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31904">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31904:SF1">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04426">
        <property type="entry name" value="Bul1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04425">
        <property type="entry name" value="Bul1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bul1 N-terminal" evidence="2">
        <location>
            <begin position="145"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="Bul1 C-terminal" evidence="3">
        <location>
            <begin position="659"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="912" mass="103256" checksum="5C3B6B926DCAC083" modified="2017-01-18" version="1">MLNFSKPFKKSNDKSLSSDAPVXKQKPSTSLSHIASASSSNMPNPNVKEKXPSVDNGIYSCDSALSELELTMSNSDVNPIFSPSYLEQTSSPTLLSEFNPVLKSSKAISVXDTNQLSKESILQTKARGATDKLSLFDHFLDRGFLVDVLPSFELDTSLHKKLTTNNSSXNDFECPPSYSELNTTSGATLDNQSTLGNREVNSDLSTLNNLYSAPQSQAQQNELIIENINFLPNKKNPSVLVHIVLINDAVKPNMPVEQESLLKEYTTGDIVXGYVTITNLSGKPVKFEMFYVSLEGYMCVYDPEXKKMTRKRFLRQIDASASWSNTRIELSSGLLYKCGGRDEFDNTILGLPNSRILEPKTKYKKHFMFKFPNQLLETNCKHNHFSHCQIPPSFGIDKFHNDREYADIQVNPILRYGHNYQRGSPILTTDFCSNNLSVNYAVEAKLVGLESKDNVQFKNERHLHVIKDTRYNIRFAPFSNPLLEFSPPNDLATCPQRQLLDLEKEVIEKLNALEKFITKVESRNNGNNVLFCLEDLNGANNNNDGDIVIVDQKLKHHYKNNKKTCKERFSKGHLENNKLILDENSVAEKTEQQNTALATLKYPYTVKSRLKSFLTQSSSDGSQSGLMRFSFKITSEKALPYKKPTLLQKRNTLQNKNKHDAQNFQFLQSHLKAGSLLRSLELNMEAHQANNTTDNVLPPEIEYIKPSLLLLTTSSIAPLPLHLTSTFLKSEVQTMKVKESFELYNNKLQSLKKLFEENSQIIKNICCNELHLERSLTFQKFISNGLAADVLSLATLSVKXDTLKNYFAKGKSNYGKDTELVFAKVLNGXSSKTGKENINERFAEEWAFVKPGVYQRKVKVNLELNKEFKDTLVPSFQSCLTSRMYIVRLEIKLKEQVGCFEVDVPVDVRFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF97</accession>
    <name>A0A1E5RF97_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Proline-rich protein LAS17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1987</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RF97">
        <property type="interactions" value="222"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF97"/>
    <dbReference type="InParanoid" id="A0A1E5RF97"/>
    <dbReference type="OrthoDB" id="8963340at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071933">
        <property type="term" value="F:Arp2/3 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045010">
        <property type="term" value="P:actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01205">
        <property type="entry name" value="EVH1_WASP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000281">
        <property type="entry name" value="Actin associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033927">
        <property type="entry name" value="WASPfam_EVH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000697">
        <property type="entry name" value="WH1/EVH1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00568">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00461">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50229">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH1" evidence="3">
        <location>
            <begin position="16"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="WH2" evidence="4">
        <location>
            <begin position="600"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="181"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="613"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="223"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="295"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="327"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="344"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="364"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="398"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="419"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="435"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="453"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="578"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="679" mass="72567" checksum="F3B0961DE0D43FED" modified="2017-01-18" version="1">MGLLTTADKEKVKIALPKAFNKIIDITIARLYIAYPDPNEWQYTGLWGAVALVDDLVGNTMFLKLVDIQGFRGVLWDQELWVNFEYNQDRTFFHSFEMEDCYAGLLFEDLNESSHFYKRVAKRQKYASKKTLNNKNAMALKKKVDEENQLNVVHGPRGETLISDQRKRYNYDLPQEVHEXDHETTQNDANDEYDFAPPTDSFSNKKAPPPPPPPSTMNTPKAESNXFQQESNSSSSAHVVATPQRVESYASMENYESTPQSESPAPVPSGSTIVHNVPPPPAQYMHSTPPLRLDTAQTGSRNETAQNGGFAFPIPQQAPSISGFGQPGPPPPPPPHPPAGTAYGQPSTPSNMGYGQPPAPPNLPSRSNMPSHLQQAYQPNNFASAPGSTPGLPSRGSVPPPPPARRGTAPPPPPRRHGTAGTPSTLQPSRTGRTGPPPPPARRGAAPPPPPRAGVRPAMAAPQAXPQVSPQPFQQPQQQQSNIPAPPMMQQSSIPAPPPPPPMMQQSNIPAPPSLPPMMQQSSIPAPPSLPPMMQQSNIPAPPPPPPMMQQSSIPAPPPPPPMMQQSNIPAPPPPPAAMGAPSNNSAASTGFSEATGDTGRDALLASIRSAGIGSLKKTDKSQLEKPSVLLQDSEPSKTGPSAPSGGGGPPSMADALAAALQSRKSKVAYNDDDDEGDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFH6</accession>
    <name>A0A1E5RFH6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2211</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RFH6"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001080">
        <property type="term" value="P:nitrogen catabolite activation of transcription from RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050797">
        <property type="entry name" value="Carb_Metab_Trans_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668">
        <property type="entry name" value="GLUCOSE TRANSPORT TRANSCRIPTION REGULATOR RGT1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668:SF4">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN DAL81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="6"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="142"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="188"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="142"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="928" mass="106992" checksum="CC13400A0654ED50" modified="2017-01-18" version="1">MRRVLACKYCRLHKRKCVHENGKPPCSYCVKMGLQDDCELSYPVVKSKLDLKMAGNESFGNEYNKMMYDSERSMAQTIQTQSNVSDYMSSLSNNVVVNNGFSNNYNNGFNTGYNGYHNSLGNNTNQCNNQFAGLANASNQYFPSSNPINTQLNNQGQNQYHYPNENPSSYNNAYSAASNSVNSVNSVNSVNTLHDSNTSNSVCSAKSASINNNNNNDNYDMISLRKSRSTSFGDSGDERTNSTQGSLQSINKYHSYGTSKTPLDLKQFLATIPTDVIVLGIEIMTHFHNGKLLLDKKYILENYNDLEPLLIGTILAFVSSNHPLKVNNFWLGANSTFVKNNTFLNLCMQDVLHGNYFETPNIQVIQALLIMSLLTLQGKLRSWLFLSTALSTIKYMLDFQDINLNTMEGEILKKTFWTSIFIKKVLFPDMNKQLVLDSEEKSYIYDMXLPYTDENDNLVVFDENSTTTENNLQNKYNQETNNNKENGNGVYFXHSTEPEKSQEHVSNNFATNELQNFKKKTHNINIDKSQNKIQTAFLDKSNKTIGYGRKKKTLTIDNIFNVDNQLNTTGKPPRNKLAYYLAFLDIYFECKRNFASLKEYEQRTNEIFTSLDMKMAFVWNHCPAQWKLNKYTKIHSRHDQLVLSINFRQKLGELFIFKSVLPCIPGNYFPELDIDAEIRKPKPLNYSSWKHYYKTFGKFVLETSEFFNKTLCVHNPKYKENAYFSEKGLMENVYDYKEGEEIEDHQNLIFDNYLKNPMLDANKIQEIKDIYSKDFLLQGNYYFYAFHEISLIANYFVEFKLPYHEEISKTNIVKNAYSFLNSSIKSTDYFKKSCNLVGRKLDYFYKIINSDFQNLKSWNLDYKDHSIVTCASKPTVFGTAHSQLGSFLTENRKVCKTKPHTLNSVFISLFTTDEMAMINDTNIFSLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFH8</accession>
    <name>A0A1E5RFH8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2188</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFH8">
        <property type="interactions" value="276"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFH8"/>
    <dbReference type="InParanoid" id="A0A1E5RFH8"/>
    <dbReference type="OrthoDB" id="3972231at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF13">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="653"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="139"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="218"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="335"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="496"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="549"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="583"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1002"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="31"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="88"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="142"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="223"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="239"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="259"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="292"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="335"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="409"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="427"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="511"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="556"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1016"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1066"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1097"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1128"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="687"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1203" mass="132761" checksum="6867B66B9AFBF7FA" modified="2017-01-18" version="1">MVSILKKFIHPNSNNEIGTKDSKTSGPTTISKNSSSSSSSTNNKLVSSNSSLSNGSKNFSSSNNHKANPVNGRDNEQVDGNAKNVGHNTSTISNDTTNANTTTNSNNSHQNNTNRHGTEKNNGVNSGHVDLQKEEKNLNFDTTTTTSSATTSSTNASGVEKQTPMINYDDVRDKDEKNGNKNGSEIKDGDSYGNDNMDDKXHLDNDTVEDLINRQKNSHNIKNNNNNNNNNNNSNNNNFTSTXTGPSSSQHEEDKKMDTMSSLENPGIKSQQGKSDNHRTTMDSGNYLAIHNGDKNDQNGQKVDDDSRPQSLGKESSSIEDMLKKDRCEIEKNMAKQGEFDXEHKGSNDISKDKTNVQGIDENKSSSNTVETTEHTKVHNKNPLKKDPEVKKPIGTNKPLLKQQQQEAKPVKKEKERKSFNPFSRLLKSSSSQVNLHSKNNNATSTTFNPALSGKSNTHHDHSFSQNRARGSTISMGQNYEMHPKNGKQVYPINTKFKVDSPSGESAPASALTQKPSTNIHPSKSLKSVSTHNTNPFMSSSNLLHNYSKNAGGKSNAATNHNSYNTSNNKMVYNPYGSISKTNTFNNDTHNNNNSSSNSLTPSGTANSLGFYLTDGIGESNLLPLPIENPNEVLPEKFKQPTVMFSDDFEPIKDGTSSIGKGASSDVRIVTNKHLCTFSKKKIYVLKKLCMFSKENPKDFYERCLKEFVIAKTLDGNKHIITTYLLMKIPTTTYMSRGWGFIMEYCDSGDLFNMIISPKWKKTGMAEKLCIFKQVAIGLQYIHSKGIAHKDIKPENILVTHEGVAKITDFGVSEMAWNNPNDPNSGVKLSNAYVGSPPYSSPEVMVFKGLNLREREKLTDKQYDPFKMDLWSLGVMFFTEMYQTLPFQSAEKIDPGYRDFLLSYSSFVSMNPRFEKDYHPAASLKSCISGPGGEYKLGKEFLDVNASRVAWKLCDPNPETRYSFEDLFSDPWFKNVQTCTIENGPEADADKHTQVEWTKMNEPLLIPPEDQYKTKPLTSPSSASLNRNDSVSSSKKPPLQNSSTFGSVSSDTPPRTPLEKVVGEKTTAPATPETIPEVPTNAQKHTKISNGSPATPDPKKPLSKKDSKAQLSQLLKDNPHPSPFAPSGSVNGLHSSDHSGGNLQNHNISRSSSIIQNSGSSSSSSSAAGPNPTVLHYISEYKEEKRKINIEYMNKKVSHNHFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFJ1</accession>
    <name>A0A1E5RFJ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2321</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RFJ1"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.50">
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ85671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="236" mass="27938" checksum="4FD50014787A0DE1" modified="2017-01-18" version="1">MISYDEYCKQLSTDLLLDLDPFQQHRVTISESSKDYIRFQFFIKHELMNTGQPXILLKCLLNYNASYNEPQLHFQIFSVDVSASFEEILDDDHEDEYTLENDMEFQYSSEQLLDPKEITFVGEPETMRFVNVFEFHRLLQSSLRHGNEHLNAYFHQRYHFPILLDSLSSGEIFYSVQNCDSHEIVGGGNAELFSVESTHQHENKGHDQHYLMKRDYLKRWCSVYILPILRDFCATI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFK1</accession>
    <name>A0A1E5RFK1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rad21/Rec8-like protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2064</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFK1">
        <property type="interactions" value="124"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RFK1"/>
    <dbReference type="OrthoDB" id="5427633at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039781">
        <property type="entry name" value="Rad21/Rec8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006910">
        <property type="entry name" value="Rad21_Rec8_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585:SF51">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN REC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585">
        <property type="entry name" value="SCC1 / RAD21 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04825">
        <property type="entry name" value="Rad21_Rec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad21/Rec8-like protein N-terminal" evidence="3">
        <location>
            <begin position="4"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="768" mass="87621" checksum="6E5DE0E8F48E521F" modified="2017-01-18" version="1">MSLSDILSSNTTLSIIWLLSNLNSGKYKTISPETDLSSAYTAKRSGRVSNKREVTNISIPKLLNNAELNNKVLKQLNLRHQSRIMYGLTMCYNKQVEFILSDLVKIKQXLFKELSTNQHQKFLQDLEKLKKLRKNEKQSGEQMIGGPFTETCIFDGTREYKTIFELQQLQLQQYGMKYDYKSLFYAKHYLDNYHETSKSVNGLLLKNDETIGVEKNPIFNCNVGGVDYSQILNIVNDVKDNYKIDTSSVDHNKLLIKQSDLSKELNIPGIADSYYLFNPLKEHQPVDQDFESDNDELSNFELSGFATSSNMLESDQFMLNATNNNSKKRKNSFDSAQSDFEIDLINLDSEPPKNGSDGNEEIQDLPFATQQSSHDMDFDLFETNRVQNAETLIEASDLPDESDDELLEPHLSSRPLLELTRNSNRFKKSFKKVKVDDSISFSNTFLRKNNESYEXNMMKIINKIEESMHFSKKHSINNDDLIIGCNLDVLKVLTSDSLRIHTSTSKHNQNLAQYDIERLRKVQSHANNENKESDSGSNTDSAVETARKFFPDKIPNASFQSDINVLADLDQINEELEHEKDLMEIDLNMGTSSPKLGALAEFRPSSFTSGTSSLMFSSQESDSFKKTSGDHLSRFQAIEEESSIGHFGLDHPHLERQSAKYFDSVQTNEFLVNRQCMKVYQYVEKKLANCLTDNISLNNLMLNDKINNQKLPRRKTVCRTFYALLQLATRDLITFEVVGNQSPISEQSHGEGFKASNGKCVFISLSRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFM7</accession>
    <name>A0A1E5RFM7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flocculation protein FLO1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2458</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFM7"/>
    <dbReference type="STRING" id="56408.A0A1E5RFM7"/>
    <dbReference type="InParanoid" id="A0A1E5RFM7"/>
    <dbReference type="OrthoDB" id="4036630at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000128">
        <property type="term" value="P:flocculation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001389">
        <property type="entry name" value="Flocculin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00624">
        <property type="entry name" value="Flocculin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184725" evidence="1">
        <location>
            <begin position="21"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="325" mass="36197" checksum="3CCD94B8650CA211" modified="2017-01-18" version="1" fragment="single">MMKLYNIVFSLFLFLCQVIAQPNDSITSTQNTVTAINTNNTNEYIIQAPHSSFYGTSYSFWNNTYNTTFSTLLITQTIQFVSTVYFVATPEQHGTYTSTTHWTGLNTATYDVSYSTFSGADGHETVDTYYFVETPMSRYISTSYSFWNGTSDSVYSTSVFTVTNGSSPFISTVYFVATPEAHATFTQTTYWTGLNTSVYSTSYSTFAGVDGHETVNTVYYVETPVSRSSSTQFSYWSEATSSTYSTAVATVTISGSPVISTVYYVVTPEGHGTIERTISWTGLETQTYSTSFSTFSGTDGHETVDTFYFVETPISRVVETSYSYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFU6</accession>
    <name>A0A1E5RFU6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ribosome-interacting GTPase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2208</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in ribosomal function.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFU6"/>
    <dbReference type="FunCoup" id="A0A1E5RFU6">
        <property type="interactions" value="1281"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFU6"/>
    <dbReference type="InParanoid" id="A0A1E5RFU6"/>
    <dbReference type="OrthoDB" id="603at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903833">
        <property type="term" value="P:positive regulation of cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01896">
        <property type="entry name" value="DRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17230">
        <property type="entry name" value="TGS_DRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001436">
        <property type="entry name" value="Developmentally-regulated GTP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000003">
        <property type="entry name" value="Developmentally-regulated GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045001">
        <property type="entry name" value="DRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031662">
        <property type="entry name" value="GTP-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006074">
        <property type="entry name" value="GTP1-OBG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43127">
        <property type="entry name" value="DEVELOPMENTALLY-REGULATED GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16897">
        <property type="entry name" value="MMR_HSR1_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00905">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="4">
        <location>
            <begin position="65"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="5">
        <location>
            <begin position="291"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00059727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ85716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="368" mass="40795" checksum="8AF893CB8DE3D776" modified="2017-01-18" version="1">MSTVDKIKSIEEEMARTQKNKNTEFHLGQLKAKLAKLKRELLAQATGGSGGGPGVGFDAKRSGVASIGYVGFPSVGKSTLLTKLTGVESEVAAYEFTTLVTVPGMIKYRGAKLQMLDLPGIIEGAKDGRGRGKQVIGVARSCNLLFIVLDVNKPLTHKQLIEKELEGVGIRLNKTRPDILIKKKEKGGLSITNTVPLTRIDADVVRAVMHEYKINSAEVAFRCDATVDDLIDVLEDQTRRYIPAIYVLNKIDSLSLEELQLLYRIPNAVPISSGQEWNLDKLLEIMWDRLNLVRVYTKPKGVMPDFSDPVVLRSDKCTVKDFCNNIHKSLVDDFKNALVYGSSVKHQPQYVGLHHVLEDEDVITILKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGE5</accession>
    <name>A0A1E5RGE5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Elongation of fatty acids protein</fullName>
            <ecNumber evidence="12">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1526</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>an acyl-CoA + malonyl-CoA + H(+) = a 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:50252"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:50253"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ELO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="12"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ85981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGE5"/>
    <dbReference type="FunCoup" id="A0A1E5RGE5">
        <property type="interactions" value="630"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGE5"/>
    <dbReference type="InParanoid" id="A0A1E5RGE5"/>
    <dbReference type="OrthoDB" id="434092at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030457">
        <property type="entry name" value="ELO_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF134">
        <property type="entry name" value="ELONGATION OF FATTY ACIDS PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01188">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6 12" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3 12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="261"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ85981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="339" mass="38929" checksum="E6FB89C7423194A2" modified="2017-01-18" version="1">MSLFASSLLEKYTPTLDHVFFNIDLFGKFDEVVNFATQGKFVPSEFQFIQGETPLATFPVVAGAIVTYYITIFGGKYVLQDSKPFVLKRVSQIHNLFLTALSGTLLVLFIEQLVPMLYKHGLYYAICDQGAWTQPMVCLYYLNYLTKFVEFTDTVLLVLKHKKLTFLHTYHHGATALLCYTQLTGETSISWVPICLNLAVHCLMYWYYFLASRGIRVWWKEWVTRFQIIQFILDIGFIYFATYQKFVYNFFPSLPHCGDCVGSPIATFNGCAIISSYLFLFIAFYIEVYRKNTKKSKIVKRTAGGVAARVNEYVQVDVKNTVTPSPSPAPRRLLKSRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGK2</accession>
    <name>A0A1E5RGK2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein SLP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1587</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGK2"/>
    <dbReference type="FunCoup" id="A0A1E5RGK2">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGK2"/>
    <dbReference type="InParanoid" id="A0A1E5RGK2"/>
    <dbReference type="OrthoDB" id="266334at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045120">
        <property type="entry name" value="Suco/Slp1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012919">
        <property type="entry name" value="SUN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12953">
        <property type="entry name" value="MEMBRANE PROTEIN CH1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12953:SF0">
        <property type="entry name" value="SUN DOMAIN-CONTAINING OSSIFICATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07738">
        <property type="entry name" value="Sad1_UNC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51469">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184738" evidence="7">
        <location>
            <begin position="23"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="713"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="domain" description="SUN" evidence="8">
        <location>
            <begin position="256"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="152"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="810"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="165"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="832" mass="92895" checksum="9EEC28B2325C323D" modified="2017-01-18" version="1">MHLNSVWKYLIILLTQTKYASADSDDGTQLTDFPPIQQDMAVENASTSHVEQLAIASTFASSESKNHFSMKALSSFPLSTSPTKKSDQPDCTLFTSQDLQDSTTFTSSSSGDQSSMFVEVVTGLQPPLTTPKEELTFNSMLSTIIGESVIQPNSSTHLRKEQQVRKNVTHSTEYPLDNVNTSQADGDQQKSQFEESSASFEDWQSGNEKKDFEVQRLEEVGPDHLAFVDHSDPLAGAIGEEMELDFDYLTSDEPSGSKKSTQSVSQVYVEDEGKIYKKKFNFASLDCAATIVASNKEAQSALSILQENKDKSLLFPCNIQSTKFVVIELCEDILVEEIEIANFEFFSATFEKIKVMASDRYPVLKHSNGNANCSCEPALQDVGSMLGSQNSSSVIPGTKKTNDNCCTGGWRVLGEFTANDTMDVQKFAIQDPQLWAKYLRVEIISHYGDEFYCPISVLRVHGKTMMDEFKQEEQIYYDEQQKGMDSELHHVEQQDEDIPKQSTTTEDLNDECFVYEGGGENLDDELFISNNLLLQYQSNVFLSNNSDNSSEKINQKICLAEFPYVKFDDFIKKNDKNSSSNGPGLQAPLQSNHRVLNSGSSAEESIFKNIMKRLSLLESNATLSIMYLEEQSKMLSQSFGRLETKYAQIMEDVVSSINSTMALNIEIMNNIVDNIRVNNELLIEKEMNAMHTEIGERINKLRRFEDELSFVKHLMYGIVIVSSFLLVYVLVTREAYLMNHLESEDSEGWYDSAGGSTSPFVRANKKFWGRIMTPSGKSKNTDALKSRGNNVDLETETSNLKRCHSVCSVSSGSSGGVENTGDVVSVGSDIDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGK8</accession>
    <name>A0A1E5RGK8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1367</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ISF1/MBR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RGK8"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031443">
        <property type="entry name" value="Mbr1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17058">
        <property type="entry name" value="MBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="24"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="75"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="275"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="395"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="48"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="80"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="92"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="108"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="275"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ86048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="461" mass="49579" checksum="E19EA32700DA84FD" modified="2017-01-18" version="1">MSPGIINKFNESLQLENVXKIDDDMMNEKDGSRNGNGLRIVTSKSDGYDTDDQGYDYNEDDSQIFERCVQDLGFDDEDTSESKNEANRESMNKATSEVNGXTSSTIDNTSTAHNNNGNSGNSGNRGSGSISSSSTSSSSSNSNSNTQAKQKADKIHRRRSNANQMMLQLSRSNSNTNTNGGSVXSSGNGYGHGSFFANSSSTPSSSATSFKSCSQFAAGNNTGSGFSVSPTLPQVAHALSRQRSSSLANSPSSIPTHLCCLERYVQQDLDSLAMSDASSTKTSTSTKSPSXEHSNDSDTMTKLNAYKLGKSRTSNDLDVTPVAVPGNPSVSTNPFYTGARSPSRSTPIAHSNWDTLNTSDEQLCESTFAFINNSRQGSKRPNYYFDKLENYAGLGNNKHTHSTEGHEENENSAIQESDGVSAEAMRPRMTRRVSLMTMALSESLPPLGRHNTAPNGMYEHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGL5</accession>
    <name>A0A1E5RGL5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Inhibitory regulator protein BUD2/CLA2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1401</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGL5">
        <property type="interactions" value="92"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGL5"/>
    <dbReference type="InParanoid" id="A0A1E5RGL5"/>
    <dbReference type="OrthoDB" id="775356at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023152">
        <property type="entry name" value="RasGAP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF60">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN RASKOL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00509">
        <property type="entry name" value="RAS_GTPASE_ACTIV_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GAP" evidence="4">
        <location>
            <begin position="583"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1125"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1024"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="17"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="34"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="51"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1136"/>
            <end position="1161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ86035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1173" mass="134030" checksum="E5B4309D4EA83B98" modified="2017-01-18" version="1">MSNIYPTRPTAAIRPINQKTPNNDESNNAKNHKSSPLVPSSSFASSTPTPKYTNRTSQHSFSKSANLGSGSRTFKINSIQELKQSFFDSNNXKNXESEKPARNFSTFEGNIEWCLEKNLPNWQLNRCFVDEYGRLIIHDGSQTTNNTKIVTPTGSKTPDIISNATDFPKEILVDHIQNYHLTLEKYNXNECPVIQLCSHVNASSSSSNNRMYHETSKNKILFRFGNDRHNFKKFFMTLIYWGNMKCCGTLEKFEIVDSKWASFCPLPLDQNXSVESICECAISFSKTEPSKRGQASSKSWEISNLSLSETTGELLIDVYEEANTGLQTFQQKLDLTKFLMSEIYVLDPSLCDSIDEHTLVIKHLPQLRKLYNIAPLTSSESPSCVYLKFKMQVDMEMWYARLIPFTKFEVLKMYGSDDSNDLRLSNNLKVVILEADLKKMAGLSTANFFVEIVIWDEVFAQTSKVTSTTSVPFWRDEFHFKFYNNINKLVLKIKQTNGLEIGHVSIDNDLLFKKGFSSEEIRLPINSFKKHSNLAGSLMLKIENQWNFVLPSANFDRLDSILKTIDLNQLIKILYSDKLSQDHKIKASSVALDVLTNLKRENEYVQLLIEKEIDEVIQLLLLKNANNTPTSETSSSQHIYNCLFRGNSNLTKVMEYYFHTVGQEYLLSILKPIVHDIISSNADCEIDPSRIRTKTPPPVFDLAGTNIDQNESADSSKSVDEIVDKHFKTLLGYAEQIWTSIYHTSKDMPQAIKNQLKTLRNNLELFSVQGLNKEEVENLIINCISSFLFLRFFCPVLLNPKIFNIISDHPSENNKRTLTLLSKLLLNLSTLTPFGFKEPYMHKFNEFIDQHKSEMXDYNDKITGKKIDFTSKTMTLSWMKGINMQSKTVFCSLSVPFLIDKCLKETEFVEILSSFYFGDRDQNHVEETTILFKSEGSNLKLSAKDDSDFRNGVASTFTNRKDNKTSLRASQTSGISDQLFEIGELEFENLSDNNTRVFGQDMLELLNLETDYKDPKNTGNGHRIEDEEKVLHELEIECRVLCAKRKRLIQKFSNYECITKDYENFLNYVVGNLYXETGSGIIKVLKPYEDSTGYKKLFYSLSSMEKIFGVYALYGTMSMDSPNNQPLSKYTKXNPVLTTASTNGNQHNSASSSPTGTTSKISRSFSRFFRRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGN6</accession>
    <name>A0A1E5RGN6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phosphate system positive regulatory protein PHO81</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1090</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGN6">
        <property type="interactions" value="210"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGN6"/>
    <dbReference type="InParanoid" id="A0A1E5RGN6"/>
    <dbReference type="OrthoDB" id="1577640at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14483">
        <property type="entry name" value="SPX_PHO81_NUC-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057506">
        <property type="entry name" value="C2_GPCPD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25329">
        <property type="entry name" value="C2_GDE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SPX" evidence="4">
        <location>
            <begin position="1"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="413"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="524"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="5">
        <location>
            <begin position="844"/>
            <end position="1151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ86061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1156" mass="130959" checksum="C6B904FE5EEF982A" modified="2017-01-18" version="1">MKFGKYLEARQLELPEYNGYFINYKALKKLIKKLSLSTSSASLADAISPNEAQLNAITNDTLSPEPNTNTSLISNSSHKNLQLNKAVFFFRVERELEKVNEYYMEREKNFKIMIEGLQEKFEEYKRKGRLASKSSSSFKYLFESLKRLQRDLNHLTQYVELNRTGFAKVLKKWDKRSHSHTKDFYLATVVSVQPVFTRSLEISQFNDLSIGLLMELDDLRQEAPLHYDAANIAASSKSAAYSTTTSKDPIRNAEDIFETNLSILSIELEIDTWYKEVNSICQLKDLEPRLQLLKXFPNKVISFINESTKLSTKENELIIKGSLNRLFYLLVSSDKVDDECLETFFLSCDGLNNHIDFLYLDNDDSYIFTKRNFFHEAAACPNHDRDFILQQALRYYTLKNSQLVKFLNDIDYNGRTPLHYASELGKAQFVESLLKTQALTTIQAVDINSNSALVLAIIRNHPSIVQLLLTSYNRDNYLLSFSNEQREQQSPLNISCIKGNYDIVLIILNTLLKDFDLKNYRDVQGYKPLHLAAEYGADTKLLELLCDHGCDPNELDSVNGWTPLIYAVSNGHVRTVESLITHCHADIKQCDDKHLSPIFYACWQGHIGVLNVLMSHEQKINLSSVNGKKDFANTNASVETSGLRTDSSDLKPTHSMSLDIDSIPDFTLPPPIIPLRKYGHNFLEQKVFLKLHFKPGFSSIKLNEEDNGLVSAANLGRITLTSNLVDLISRNISLPIESSSSYYYTELGFNSNQSNNVTNLSSGNDVDSTDEVVFQINNLNNCNIDLEIYPVSGTRMIAKTILTYDILRNNIDSAVNLGSVVLPLLDSRLKLVGQIHFDFQVIYPFTNMPLEITQFETYWKSTGEGQMTSSSLSGEYVTILVTLLNDGKIVAVPKFTIQQADTEFLLMDLSAHQVESIIGRRLSDLSGATHDYKLLQEFIHNNYICFESLLKALPSDVKIGIKVAFPTDAEVSQVPVKLTSPYINIDNFVDDVLSITLNYIQSTQSMSSKKVVFSSSNHMICSILNWKQPVFPVIFNMNGVFKNAGSNSFAIESQNHLIQFALKKNFNHNDFNYRSVKDVVNFAKMNNLLGVIIPTELLLLGDSLIAEIKASGLLLIAGRSSKSETNENDLWNHPKINGIKNNRCLKFKDQNNEFIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGR9</accession>
    <name>A0A1E5RGR9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1662</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGR9"/>
    <dbReference type="FunCoup" id="A0A1E5RGR9">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGR9"/>
    <dbReference type="InParanoid" id="A0A1E5RGR9"/>
    <dbReference type="OrthoDB" id="545683at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01899">
        <property type="entry name" value="Ygr210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.470:FF:000001">
        <property type="entry name" value="GTP-binding protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013646">
        <property type="entry name" value="YGR210-like_G4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF1">
        <property type="entry name" value="OBG-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08438">
        <property type="entry name" value="YGR210-like_G4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="3">
        <location>
            <begin position="5"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ86100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="436" mass="48860" checksum="96CFBB7F9FE4D278" modified="2017-01-18" version="1">MVKEFCVAFVGKPSAGKSTSLNALCLIDAHEKFKTGNYPFTTLEAAEGTGFVKVPCKCRDPVVKDLLAKQGIDIKTVQCKPNYGWCEDGIRNIPIKLLDIPGLIPDAHLGHGLGNKFLDSLRKADALVHCVDISGTTNEKGEACRAYDPINDVDWLVDEIKYWIFNNLKNRWGSIVRRHTASKSTTVETLSQQFGGYSANLSMVQKALDKFPNNELPPLQEWNDEWILKVIEKFMSVKFPMVLALNKIDHQDADKNISKVMLKYGNKYKIVLTSAVTEVFLKKLKYQGFIKYEEGTEFVDTYEDDNTLKPLPENIIERIENIRDLVLYRFGSTGVSNVLKTAACDTLDLMPVYTVKNINNFSNELSSNSSNSKNIFRDCFLVKKGSKVGDIKRHLFGIEVSIGAIMTVNNVRVGEDDVIMPGKNDILSFKLAPKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH30</accession>
    <name>A0A1E5RH30_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Type 2A phosphatase-associated protein 42</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RH30"/>
    <dbReference type="FunCoup" id="A0A1E5RH30">
        <property type="interactions" value="646"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH30"/>
    <dbReference type="InParanoid" id="A0A1E5RH30"/>
    <dbReference type="OrthoDB" id="10261753at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051721">
        <property type="term" value="F:protein phosphatase 2A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035303">
        <property type="term" value="P:regulation of dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.540">
        <property type="entry name" value="TAP42-like family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038511">
        <property type="entry name" value="TAP42/TAP46-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007304">
        <property type="entry name" value="TAP46-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10933">
        <property type="entry name" value="IMMUNOGLOBULIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10933:SF9">
        <property type="entry name" value="IMMUNOGLOBULIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04177">
        <property type="entry name" value="TAP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="394" mass="45327" checksum="BD8E446C8400F8B7" modified="2017-01-18" version="1">MSIVLKEYNTIVKQIKTKIASTGLRQDAKEFQDTLSSLFQQLLALKQKVFNDFAYFSDNESLDDIASKSLKFLSIDYYMAYLMSKKQAVLSSNNNASSFMLSNKNKLKIEFLSKSVQLYMQFLVTLNDYEILDKNMSKKLNGFASVFKPTLDEITNKNVPNNQAFQRNMKIENFKVQKELDTRLRLLENKILEKTGKNASNNGDAEIDDDDDDDTVDDELIRELYLAKLTQLSYRAFTEIEGNLYEVEMLSNFTSMDSIADHHAEMEKQSEEKQKREQDPTRYTEKLEILNQPLLSKTGKVLRNFTLLNDKQSQQKKVFGFGQYGPTMSVEEFLEQEFASGNVLQGGGQDPSLMDNKANEDSEEWADKETYKAREWDDFTETHTKGSGNTMNRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH39</accession>
    <name>A0A1E5RH39_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">phospholipase D</fullName>
            <ecNumber evidence="2">3.1.4.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1408</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14445"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.4.4"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.4" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH39"/>
    <dbReference type="InParanoid" id="A0A1E5RH39"/>
    <dbReference type="OrthoDB" id="14911at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09141">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896:SF76">
        <property type="entry name" value="PHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00614">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="7">
        <location>
            <begin position="407"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="7">
        <location>
            <begin position="707"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="8">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="8">
        <location>
            <position position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1062" mass="123871" checksum="4EAAF073C5EB31B2" modified="2017-01-18" version="1">MRPEINKVKTTNVTANANTVVEDPLALSFAKRLKNHSKEMVFARKNIDFDNLKLMFETLTFGIEKKNKDNIGFYYNVTLKTQDRARAIALETNLTSECLFAFLRILENEKPERVRISTEHQKFSSSDLSLRIFLGNSHFTSIGILFKXILTKSLNSLETHEMKRKLMTRVLEVFETSITEFHYCHPTIYIKHNCTGFLLTHXLQKKMKNICLLLGTEWIAYSDGPGHTILHDVFYLDSNCKVCKFEYQDKAGLTLEKYKEKLXFLLPDDETRDNYFKHIEQIXXDKKKAKQNRFSSFARIRSKNLVNLXLNGDDYFKKLYKQLDNANFSIMIQGWWISPELYLLRPIKGNEQSRLDXLLKKKAAEGVKVRIMTYHEPHFFLSNNSKHTEDILSNLHPNITVIKNRXGDFSHHEKVVIIDYEVAYLGGIDLCFGRYDTCNYCLTDAATELEKQHYPGKDYSNARYKDFGNLDEPFVDQLDRXXDPRMPWHDVQSEIIGHAVKDVAFQFIQRWNFLVKSKYAGKEISMILYPEENNADFYERNLKAFGKPEDQFLRCQILRSSANWSSGLPVKECSILKGYVHMIKKSKRNIYMENQFFITSTRENIALIQNDIGXAIXQRILEAVAENQEWVALIVIPLLPAFTSEVDSANGTSLRHIIARQNMSISYGPNSIFQKLRDVGIIPEKYILFTSMRTWGEIGPESKLVTSQIYVHSKVIFADNDKCIIGSANINDRSMLGDRDSEIAVFIESEMLTEIKVCGVTYFIDNTISGWMQQLLREKFGCYLDLVKIVEKQFEKYKQEAHNYARQFSLDPNSKSERIQKLLAFFHFEFAMRGVLKVECTERWKPFTTGEPSRTLPLTAYERTPFTFFAQKEHVWKISSLAGFFTYLNTSNASDSQKVDLLKRVQYLQLATLEKRKQGFDIFKPLNKTPSNTSDDLFFPPFSKELFQRAKLTDPFMFLDPTMSFVKDYWNKIAAQNTEIYRNTFSCQPDDNVQSWKEFKKNKERESPKNTEQKNALRKELQKTQGFVVLAFTKYFALEMLNNNFYFTRDLENFQRVKIIHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH80</accession>
    <name>A0A1E5RH80_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein eS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RPS1</name>
        <name evidence="8" type="ORF">AWRI3579_g1202</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (25S, 5.8S and 5S).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the eukaryotic ribosomal protein eS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RH80"/>
    <dbReference type="FunCoup" id="A0A1E5RH80">
        <property type="interactions" value="1386"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH80"/>
    <dbReference type="InParanoid" id="A0A1E5RH80"/>
    <dbReference type="OrthoDB" id="9834376at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03122">
        <property type="entry name" value="Ribosomal_eS1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001593">
        <property type="entry name" value="Ribosomal_eS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018281">
        <property type="entry name" value="Ribosomal_eS1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027500">
        <property type="entry name" value="Ribosomal_eS1_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11830">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01015">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01397">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01191">
        <property type="entry name" value="RIBOSOMAL_S3AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="initiator methionine" description="Removed" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="modified residue" description="N-acetylalanine; partial" evidence="6">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="255" mass="28740" checksum="5D767DD5D8B02E82" modified="2017-01-18" version="1">MAVGKNKRLSKGKKGLKKKVVDPFTKKEWFDIKAPSTFENRNVGKTLVNKSTGLRNAHDYLKGRVVEVCLADLQGSEDHSFKKIKLRVDEVQGKNLLTNFHGFDFTTDKLRGMVRKWQTLIETNVTVKTADDYVLRVFAIAFTRKQPNQVKKTTYAKSSRIRAVRKAVSEILTREVSSSTLSQLTSKLIPEVINKEIINATKSIFPLQNVHVRKVKLLKQPKFDLGALMSLHGEAAATEKGKKVAGFKDEILETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH81</accession>
    <name>A0A1E5RH81_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">RHO1 GDP-GTP exchange protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1321</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH81">
        <property type="interactions" value="294"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH81"/>
    <dbReference type="InParanoid" id="A0A1E5RH81"/>
    <dbReference type="OrthoDB" id="2272012at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04435">
        <property type="entry name" value="DEP_fRom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041675">
        <property type="entry name" value="PH_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052233">
        <property type="entry name" value="Rho-type_GEFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46572">
        <property type="entry name" value="RHO1 GDP-GTP EXCHANGE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46572:SF2">
        <property type="entry name" value="RHO1 GDP-GTP EXCHANGE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15405">
        <property type="entry name" value="PH_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="4">
        <location>
            <begin position="663"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="5">
        <location>
            <begin position="1209"/>
            <end position="1538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="31"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="127"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="172"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="227"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="548"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1052"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1351"/>
            <end position="1383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="137"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="177"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="233"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="308"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="341"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="360"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="385"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="400"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="553"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1052"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1063"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1084"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1351"/>
            <end position="1363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="1364"/>
            <end position="1374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ86260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1559" mass="175052" checksum="155FEDCC8643F462" modified="2017-01-18" version="1">MTAINTGTHHQRLTDVQKKIVFKEIFGISRSASPRSNGSSHITANTDISLPSTETTTTTTTTNIHEICEKIDLNANPLDLLNLSPLNTVLVEEDDEYXSLDPLSSPPNIVKNNTIDRLNKVDLIHDKHNDHNMKVGNRNNSSSSSSRSNSYNSITQNPIIQKTARRLSLYNSSNAHNSKNNTVNNGNNKSFVNESPLSSTASSSTSSSPVKKRSSFEFIAQHMKFRKSESPVNDDNAPTITTNNNINDNNYTTSMPNYNNTTNNNNNNKNTSTNNYKTPGNRDSSGNANGSYSFLKNINTTIKRKTSHSSLNNTPTSAISMNNPTKHNRIVSNNSLVSPHSRSHSRSHSRSHSYTHVHNHSQSNIQSNRRTSKSSLVTESTLASPHHHHHHNHYNHIHHGSTSSHHNNSQNHINNSSSSVSGSSRRALVNPALLSKVAHKFRKYIQLTENKKDGIVYRDSFKGVQAVDLLCSMLKTNDRNLALLLGRALDAQNLFHDVVYEHRLRNSNNEVYEFYSEETMADPSSDTATNXSQVSSNVTLDQVGISSPKSILSNSETTSSGNTSNKHTSNVNKINGVFTILTECYSPSCTKDRLCYSISCPRRLEQQARLNIKPQAVLKRNITMSLNELEDDHLNHYSGEEKQSWLSSVPKEIWERLDKTEIKRQEAIYEVYNTEKNFIKSLETIRDNIIKTLAETNIIPADIRKNFIKHVFAHINDIFSVNRRFLESLTERVKKAPIIEGIGDIMIKWIPFFEPFVFYIASRPYAKYLIETQRTVNPYFSRFDEDLMSSKLRHGIDSFLSQGVSRPGRYVLLCKEILKHTDPKTYRRDYDYLSKAVEQLGGLMKRIDKASGAAQDRHDVILLKQRILFKNEYVNLGLNDEKRRIRHEGVLSRKEISKGNDFYNNFQSVGDIQFYLLDNVLLFLKAKAVNKWQQHKVFQRPIPLPLLFAIPGEEMISLRKHIGSHPGCSACVLNPRQEFNGLNSNFLSNNDSFINSSGLANAVSTATPTSTTAPSIFASTVKSAAGIGKSSANGSTNKLEVLTALNTSTTNSTLITTGTSNNPTTPHTPLSSFNTASQLFNLHNNNNSNNNNNNNNNNNNNNNNNNNNNNNNNSTNEHSNNTLTLGSPLIHANSTNTLAGTPLTSSTGSNAITFLYYGAKQRYQITLYCAQFAALQTLLEKIKQEQQRLLDNNHIFNVYKISDKFFDYSNRIVGLSTFDSGRKFIIATSSGLYVSNAKKSSQTKRVMLSAPIKLLAKANIQQISVLEEYKILLILMDKKLYSFELELLNDLVNSSAASSLQFFKKYSKELMTHVNFFNEGECCDKKLIVTAHGSSHVIKFFELSNQIRGNASSHSTSSSKNGGKTSGTGIGGSHGFMSKSNTNNKLSKSKLKITEVNFESEPVSISFLKNNLCIGCKKGFQIVSVTQNAHEPLLDPADTSLEFALKDMLKPMEIYRVGNNFLLCYQEFAFFVNSQGWRKKESHIIFWEGVPQKFAMWYPYILAFDSNFIEIRKIDNGDLIRCIIGEKIRLLKANSQEILYVYEDDRGYDTIASIDFWMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHH9</accession>
    <name>A0A1E5RHH9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHH9">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHH9"/>
    <dbReference type="InParanoid" id="A0A1E5RHH9"/>
    <dbReference type="OrthoDB" id="10261522at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044715">
        <property type="term" value="F:8-oxo-dGDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03676">
        <property type="entry name" value="NUDIX_Tnr3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000019">
        <property type="entry name" value="Thiamin pyrophosphokinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031804">
        <property type="entry name" value="DUF4743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622:SF8">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15916">
        <property type="entry name" value="DUF4743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="155"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="340" mass="39481" checksum="9C35415BC5B5EF10" modified="2017-01-18" version="1">MSKVVNLRGRQVLERTKEDKATNFSYLSIIEKTDNVPLNXESFPNFQDHVVYLESHNGAFKTGFVLKFTIDLMVKHAKGLFEKVFQLQDNCLRFKSSNFEERNRGIDELAHFLRQKEIFDCLKGWRNEKYVMYDLNKKPYVLVERALTNIFGIITYGVHINGAVIDQENHINFWIPRRSXTKQTWPLKYDNIIAGGLGYPYGIHETMLKECMEEGNLSEELASKYTKPAGCVSYLYHSGDFKSDTFSNEGSSITGEVEYIYDMFLPSHIIPTPNDGEVESFKLYSLQETLDLLKSDEFKPNCALIFVDFFIRHGYITPENEPNYAEIVRKMHRTLPFPTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHJ8</accession>
    <name>A0A1E5RHJ8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein SSH4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1453</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHJ8">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHJ8"/>
    <dbReference type="InParanoid" id="A0A1E5RHJ8"/>
    <dbReference type="OrthoDB" id="258495at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="3">
        <location>
            <begin position="206"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="549"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="549"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="579"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ86388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="597" mass="66502" checksum="CBF2DD06E51BD528" modified="2017-01-18" version="1">MGTHYHLLLIKRNVKALFFDMKNETQKGLDTSQTNPSFPSPYYPGDDYGDPSLGDNVSVVFLISLTVTLGMMLLVLLLIVIYVTCINSDDEEYDQEMGVSVIGTDGVRRPVRGLGALFNRASTSSSRNSRNGASDLVSFGSGPINLNDDNFKVPGSFDDSEELRTQERTYIEGGKFSDFEIELYYRGKEFQQLNPPLVKPFNTYNSVADKQLIKDRGVEAFYFLPSINDKVTREGSFLPSFIVQDKLDITFTRFNESASTILNFPLPFNGKDAVYFEVKVFKFPPQSNSIFSIGLMTPPYPYFRLPGYNKYSIAYESTGKLRINNPFHADTLLPKIQEGDVIGFGYRFRTGTIFITHNGKKMMDLTHNYKADMFIGIGCMNSAYTRTYTRDGMLEDPDNVTLKNNNGSTLPLILQNVHNPKVDDVSSDEVELHVNLGQVGFVFIEANARKYAFSSPHGTIGVPPNYHAEEIKTDKLLQKGEELPPKYPIDEMGFFGNISVNMHGPSLLFSQSXKSXQAQTNKGQQLKVKSVTEGSSLDLIGGNCLXESSSASGNPAASVDSANLTIANSTNATQEQVKHTKHSKRHKKKKGKKSRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHL2</accession>
    <name>A0A1E5RHL2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g1450</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHL2">
        <property type="interactions" value="340"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHL2"/>
    <dbReference type="InParanoid" id="A0A1E5RHL2"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060237">
        <property type="term" value="P:regulation of fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11651">
        <property type="entry name" value="YPK1_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000048">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="537"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="15">
        <location>
            <begin position="793"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="163"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="287"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="66"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="92"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="163"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="192"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="318"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="338"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="570"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ86392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="859" mass="95095" checksum="B00C2321E7119BAB" modified="2017-01-18" version="1">MFSSWKIGKKFNFGSNSNDKHHDKHHQTEKNSQKLDESVSSSKANSSGQSKDSSSNGNIPDRRQTIVATPQSMAPTRLSTEVPRPSVDSSRTTHTQNIITTTSELASNHSSSNTSATQQPNSSRQSVSTPSKSKSNSSALLNGDYQDGVAKMHSQNSSSVFLNQKNNSTEHPTSNVIPEAEQVPPPPPPPQTASKESSAVPSAFSQQEQTNNILHTQNEDNSHASSQSGIMTIKVYSGDSFKLPFPIIYNQQLLSRLLSSSNSKQAPNLMDDLNKYMEILSLNNAGKQAANGNDRGGSMSSMSSSSGASSTVLNSGSGVYNLQQENNXGNMSAMGTAKSDSMNSSVTADDGAKGSVSNNSFKHGQENIIPGDVATHYIPSAITLPGSSDLNPLIYFTIEFDNTTATIEPESGTMNHPVFNKISTFDVTRKLPFLKIDVFCRIPSILLPKDLLNNANETPGNEDLGSNSLNEMVHKIXNNQDVHLITYNLPLNLNFTTASNIRIYNHKWININELGTKLNLTIDYKPLSNKKLSIDDFELLKVIGKGSFGKVMQVKKKDTNKIYALKAIRKSYIVSNSEVTHTLAERTVLAQIQCPFIVPLKFSFQSKEKLYLVLALINGGELFYHLQKEGRFTLSRSRFYTAELLVALESLHALNVIYRDLKPENILLDYQGHIALCDFGLCKLDMKDKDKTSTFCGTPEYLAPELLLGEGYTRVVDWWTLGVLLYEMLTGLPPYYDEDIPTMYKKILQEPLKFPEGFDEDAKDLLVRLLNRDPRKRLGSNGAHEIKSHAFFSQLSWKRLTMKGYIPPYKPPVANSLDTSNFDSEFTKEKPVDSVVCDFLSESVQQQFGGWTYVGNEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHL9</accession>
    <name>A0A1E5RHL9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Metal homeostasis factor ATX1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1566</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ATX1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHL9"/>
    <dbReference type="FunCoup" id="A0A1E5RHL9">
        <property type="interactions" value="571"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHL9"/>
    <dbReference type="InParanoid" id="A0A1E5RHL9"/>
    <dbReference type="OrthoDB" id="689350at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016531">
        <property type="term" value="F:copper chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006825">
        <property type="term" value="P:copper ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000008">
        <property type="entry name" value="Copper transport protein ATOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051881">
        <property type="entry name" value="Copper_transport_ATOX1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46365">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN ATOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46365:SF1">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN ATOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-0187">Copper transport</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="HMA" evidence="8">
        <location>
            <begin position="3"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="72" mass="8077" checksum="BCC73DCA9C6A6BDC" modified="2017-01-18" version="1">MPSNHYQFNVKMSCSGCSNAINRVLTKLEPEVSKIDISLEKQTVDVITTLPYETIYEKINKTGKEIVSGKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHS0</accession>
    <name>A0A1E5RHS0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">60S ribosomal protein L14-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1384</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eL14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHS0"/>
    <dbReference type="FunCoup" id="A0A1E5RHS0">
        <property type="interactions" value="1140"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHS0"/>
    <dbReference type="InParanoid" id="A0A1E5RHS0"/>
    <dbReference type="OrthoDB" id="1875589at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23702">
        <property type="entry name" value="eL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000030">
        <property type="entry name" value="60S ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039660">
        <property type="entry name" value="Ribosomal_eL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002784">
        <property type="entry name" value="Ribosomal_eL14_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01929">
        <property type="entry name" value="Ribosomal_L14e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="KOW" evidence="7">
        <location>
            <begin position="14"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein eL14" evidence="8">
        <location>
            <begin position="51"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="135" mass="14756" checksum="23593E614054A338" modified="2017-01-18" version="1">MSSVKASNWRLVEVGRVVLINQGPSAGKLAAIVEIINDKHVLIDGPETSVPRQSVNLGHVVLTPLTFALPRGAKTGTVSKKWATAGVSDKWAASAWAKKIAQRQRRAALTDFERFQVMVLRKQKRFSVKKALAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI53</accession>
    <name>A0A1E5RI53_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1516</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI53"/>
    <dbReference type="InParanoid" id="A0A1E5RI53"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="270" mass="29619" checksum="1177222B894058EF" modified="2017-01-18" version="1">MFKLYGKSTTDNASFQGHRASGGESQTSSLAHVDLSATVSDSNSNGASSLKYSVGSLSSSAGMIAAPSPQHSQYTFSRWDSFEQVSEPADTENEEFAVLSFSTGSDAESGSRSYSLLSHPVSESRSQSLNNGPATFGLATKQAKSVTPAYVISLSDSEMASARLGSQSALSLLGTEKYSGKNLQNDKPNDSYRFRVMKSLDHIDQQKPKFHRYTRGDFQLIFEYLTAKKSQLQKKQQQSFQQNQTRVRNIPSVFGEHKDLLFQLSLEMST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI96</accession>
    <name>A0A1E5RI96_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="8">PPIase</shortName>
            <ecNumber evidence="8">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1184</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="8"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI96"/>
    <dbReference type="FunCoup" id="A0A1E5RI96">
        <property type="interactions" value="378"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI96"/>
    <dbReference type="InParanoid" id="A0A1E5RI96"/>
    <dbReference type="OrthoDB" id="193499at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000032">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF327">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE C, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0697">Rotamase</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="9">
        <location>
            <begin position="31"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="192" mass="20869" checksum="6598406AD8054999" modified="2017-01-18" version="1">MFASSIITKRAVPLTSRFFSSSSAVLGTKVFFDVSINSKKLDRIEFELYDDVTPKTCENFKMLCTGEKGWGYKGTPFHRIIPNFMIQGGDTDLTNGFGGKSALTGGKFDDENFVKGHNKVGLLSMANAGPNTNGSQFFITTNPDLSYLNGKHTVFGEVVKGWETVEAIEACGAMSGQPRAKVVIEESGEVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIB5</accession>
    <name>A0A1E5RIB5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">5-formyltetrahydrofolate cyclo-ligase</fullName>
            <ecNumber evidence="5">6.3.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1169</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(6S)-5-formyl-5,6,7,8-tetrahydrofolate + ATP = (6R)-5,10-methenyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10488"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5-formyltetrahydrofolate cyclo-ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.2" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RIB5">
        <property type="interactions" value="211"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RIB5"/>
    <dbReference type="InParanoid" id="A0A1E5RIB5"/>
    <dbReference type="OrthoDB" id="3970891at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030272">
        <property type="term" value="F:5-formyltetrahydrofolate cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009396">
        <property type="term" value="P:folic acid-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002698">
        <property type="entry name" value="FTHF_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23407:SF1">
        <property type="entry name" value="5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23407">
        <property type="entry name" value="ATPASE INHIBITOR/5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01812">
        <property type="entry name" value="5-FTHF_cyc-lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="165"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="178"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="7">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="7">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="7">
        <location>
            <position position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00036539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="343" mass="39198" checksum="102DEB3CBB030371" modified="2017-01-18" version="1">MLGTVSKHALRKELRKKLKKLGQDELNKQSHTVLHSLQKILDQLVSPTTGPFRKECAQGTHVGVACYMNMDDSEIKTLDIIKYLFNHNTNEGQKYKVFLPRCTSTKETNQINFRSDEDLKHQDHLTFHHMVSFKSVVELKPVGKYKLREPQVDFGKGIYEMNQDNIKSDNKSDNNHSTTITYKESSDXTSVKXSGIKGKPIDLCTKKXNDSDASTKHIHVTHEKVNDKINLYHPPPKDIQIIIVPGLGFNVQQMSRIGHGKGYYDDYVKRHIHYHQNSDDSGNTNKXVEERPVLIGVCLKEQIVEQQLPLEKHDYLMDYIIDGSGEIYXNEASKAXDESITNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN41</accession>
    <name>A0A1E5RN41_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g876</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN41"/>
    <dbReference type="FunCoup" id="A0A1E5RN41">
        <property type="interactions" value="78"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RN41"/>
    <dbReference type="OrthoDB" id="4092812at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184811" evidence="2">
        <location>
            <begin position="20"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="136" mass="16001" checksum="A4C7E8C35D227BE3" modified="2017-01-18" version="1">MNFIIFLIAVFFLVLFGLALPYFSNLGTYNIKTTYSNNKKNKDFDNKQRESHKRKKLNFSLNVDSEETLQKKYIVDGKTGLKKRVVGDFNQDPNQFDYDVDELIREDEEEEQRQENLKYRRNKSTNGASSNVEELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNG6</accession>
    <name>A0A1E5RNG6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">DNA polymerase</fullName>
            <ecNumber evidence="21">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">AWRI3579_g944</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Catalytic component of DNA polymerase delta (DNA polymerase III) which participates in chromosomal DNA replication. Required during synthesis of the lagging DNA strands at the replication fork, binds at/or near replication origins and moves along DNA with the replication fork. Participates in leading strand synthesis during replication initiation and termination. Has 3'-5' proofreading exonuclease activity that corrects errors arising during DNA replication.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 21">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 21">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 21">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 21">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="21"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNG6"/>
    <dbReference type="FunCoup" id="A0A1E5RNG6">
        <property type="interactions" value="786"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNG6"/>
    <dbReference type="InParanoid" id="A0A1E5RNG6"/>
    <dbReference type="OrthoDB" id="2414538at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008296">
        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006287">
        <property type="term" value="P:base-excision repair, gap-filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045004">
        <property type="term" value="P:DNA replication proofreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05777">
        <property type="entry name" value="DNA_polB_delta_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05533">
        <property type="entry name" value="POLBc_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.690:FF:000001">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.730:FF:000004">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000004">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.730">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050240">
        <property type="entry name" value="DNA_pol_type-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025687">
        <property type="entry name" value="Znf-C4pol"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00592">
        <property type="entry name" value="pol2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322">
        <property type="entry name" value="DNA POLYMERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322:SF23">
        <property type="entry name" value="DNA POLYMERASE DELTA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14260">
        <property type="entry name" value="zf-C4pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 21" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 21" id="KW-0235">DNA replication</keyword>
    <keyword evidence="17 21" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="14 21" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="13" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15 21" id="KW-0408">Iron</keyword>
    <keyword evidence="16 21" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6 21" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18 21" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 21" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 21" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="23">
        <location>
            <begin position="272"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="22">
        <location>
            <begin position="572"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="domain" description="C4-type zinc-finger of DNA polymerase delta" evidence="24">
        <location>
            <begin position="1034"/>
            <end position="1105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00055225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF14260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="OEJ88437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1122" mass="126799" checksum="385D2C3267736127" modified="2017-01-18" version="1">MSKRPNVGENIALLNENDVEMGTADVETHFTNNKRLKSFDHSTLSEPLNSINLIPSNDFVKHKTTGYASSTQDTSAKGLTNANTVSSFEKELQTMANISPSKAKTPNSKLNNRQVWSRKPLPLDFDCSTHDISFQQLDAEEATVSGFQDNNTSAVVRFFGVTDEGHSILCNVTGFKHYLYVPCPPEFDASSHNVEQEIASFLKSLNDQYEREAGKIDSIKVVLKQSIWGYSGDSKLNFFQIFVKEPYTIHKLRTGFEKGYVQYNGWYSQGCTTYDNIAFALRLMIDCGIVGMSWITLPKGKYRMVEDPMDKISPVQLEVSINYKDLIAHPAEGEWSKNAPMRILSFDIECSGRVGVFPEAQIDPVIQIANVVSNQSENKPFVRNVFTVNTCAPITGSEIFDYAKEEDMLMAWKKFVVEVDPDVMIGYNTSNFDFPYLVDRAQALGLHEFPYFSRLYHSRQKIKDAVFSSKAQGTRESKTINIEGRLQLDLFQFLQREYKLRSYTLNAVSAHFLGEQKEDVHHSIITDLQNGDSETRRRLAVYCLKDAYLPLRLLEKLMSLVNYIEMARVTGVPFSYLLSRGQQIKVVSQLFRKCLQIDTVIPNMNSQGSDQQYEGATVIEPIRGYYDVPIATLDFNSLYPSIMMAHNLCYTTLCDKQTVDRLKLVKDKDYIITPNGDIFVSSKLRKGILPEILEELIGARKRAKKDLKNETDSFKKNVLNGRQLALKISANSVYGFTGATVGKLPCLAISSSVTSFGREMILTTKNAVEKKYSLENGHTHNAVVVYGDTDSVMVKFGTTDLKESMKLGTEAALFVSGLFKHPINLEFEKCYFPYLLINKKRYAGLYWTSPEKYDKLDQKGLASVRRDSCPLVSIVMNKVLKGILIERNVQGSLDYIKQIIDNILQNEIDISKLIISKGLAPDYTNPQPHAVLAERIRKRDGMGPNVGDRVDYVIIGGNGKLYTRAEDPLFVLENNIPIDSKYYLNNQLQNPVVSIIGPIIGEKKAQAMFVVKSIKITTGKTGALMGFIKKVETCKNCKSPLRNEPGPLCRNCASKTTEIYLKSLYEVRDLQEKFSRLWTQCQRCSGSLHNEVLCSNKNCDIFYMRVKTKKELQDKSESLSQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNU1</accession>
    <name>A0A1E5RNU1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">[RNA-polymerase]-subunit kinase</fullName>
            <ecNumber evidence="2">2.7.11.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g904</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.23" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNU1"/>
    <dbReference type="FunCoup" id="A0A1E5RNU1">
        <property type="interactions" value="1078"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNU1"/>
    <dbReference type="InParanoid" id="A0A1E5RNU1"/>
    <dbReference type="OrthoDB" id="1732493at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070985">
        <property type="term" value="C:transcription factor TFIIK complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07841">
        <property type="entry name" value="STKc_CDK7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000097">
        <property type="entry name" value="Putative cyclin-dependent kinase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037770">
        <property type="entry name" value="CDK7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF0">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="7"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="13"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 11">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR637770-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR637770-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ88555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="313" mass="36033" checksum="B307E715C2FA69CD" modified="2017-01-18" version="1">MSNLVSYTKEKKVGEGTFAVVYLGHQQNGPTPNRRVAIKEIKTSSFKDGMDMSAIREMKFLQELRHVNIIELVDVYNENENLNLVLEFLPADLEMIIKDKNILFGQADIKMWILMTLRGVHHCHRNFILHRDLKPNNLLLSPDGQLKIADFGLARAIPMPQDILTSNVVTRWYRAPELLFGGKHYTYSVDMWSVGVIFAELMLRIPYLPGKDDVDQIDLTFRALGTPTDRDWPEVSSFPSYNRIKIYPPPSREELRKRFIAATENALNLMCGMLTMNPQKRWDVAQCLQSPYFIELPEPTDPIELPKLTKNEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNW0</accession>
    <name>A0A1E5RNW0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein SQS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g589</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNW0"/>
    <dbReference type="FunCoup" id="A0A1E5RNW0">
        <property type="interactions" value="287"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNW0"/>
    <dbReference type="InParanoid" id="A0A1E5RNW0"/>
    <dbReference type="OrthoDB" id="21470at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051189">
        <property type="entry name" value="Splicing_assoc_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14195">
        <property type="entry name" value="G PATCH DOMAIN CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="2">
        <location>
            <begin position="731"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="781" mass="88824" checksum="11A5D89226CFEBAB" modified="2017-01-18" version="1">MAKRKSRNNGSSKLASSQKGKKSNKKSKSNGTSKRGKSIFNFNRDEGGDQLFDTRPAKVGKYGGFSNLRLGGGDLANPDFISDFYFGRNNMKKGSMKMSAYRNKPKHIGRRYGDDDDSALDSDDEYEREKRNVPARKRPVLFVKALEVYDPSKELIEMLRAKNNVENNYMGTLDQNMEASDNGDEMKDFESVADQYQLGEDVESDSIDYKDVYGDEESGSDGYETEGDDIYNYDEDVYQNQISEESRKENIPNTEENALFFIDEEGTDCNPEDVKVVQVEPSKPAVSISTEFNSVLTIGKIQMNLENDPATNEVHSIPIRKKVPKQTLDQETAQSSKLSESFNDLEIEDLSTINSPPAAFSKHNPLDSKHRNKEVGVEFGLLDCDYVANTSDVEVTNIRLGSNTQSYYVKCYKFFGSYDFNWCDEDFFVDVLNELGLPTDRHDAYFQYIYKSLVPEEDERENSNYDHLYMTDSEISSDDEIDVNKEYGDYTLPVQESDSLEENDSITSDQYEGLDDLISISQTHARARNQDFETTSVRTIGKDKKKKLVLENEFLDEETKSLLQEKYKVRLENKATKTKNVLEFIDEMNKTSTDLLLKYPYGLHIKNIKEEYKLFLNDLSRQRLSFPPLDPHGNKTIEKMGKHFNLKISKRTSKSYKYITCEKTKKTDKKGPNYQLVDQLLKQRPVFMRVDRKLPANMAIKIKDEATGSTRSKFGVKEGEVVGGESSAIDSSNLGHQMLRKLGWSSGQGLGKQGQQGIIEPLQVLVKKSKRGVGHQQVPAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP18</accession>
    <name>A0A1E5RP18_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Mitochondrial outer membrane protein porin 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g820</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic mitochondrial porin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP18"/>
    <dbReference type="FunCoup" id="A0A1E5RP18">
        <property type="interactions" value="1056"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP18"/>
    <dbReference type="InParanoid" id="A0A1E5RP18"/>
    <dbReference type="OrthoDB" id="7827681at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008308">
        <property type="term" value="F:voltage-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07306">
        <property type="entry name" value="Porin3_VDAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="entry name" value="Porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001925">
        <property type="entry name" value="Porin_Euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027246">
        <property type="entry name" value="Porin_Euk/Tom40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11743:SF70">
        <property type="entry name" value="GH26960P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11743">
        <property type="entry name" value="VOLTAGE-DEPENDENT ANION-SELECTIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01459">
        <property type="entry name" value="Porin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00185">
        <property type="entry name" value="EUKARYTPORIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0626">Porin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ88453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="280" mass="29391" checksum="1E97F2DBF45A8418" modified="2017-01-18" version="1">MAPVFFSDISKNVNGLLNRDFYHGTPASVDVKTTAPNGVAFTAKGKTNPKTGAIASSVEAKFSDKQTGLTLTQSLNNASALETKVELADLTPGLKAELATSLIPGSSKAVKLNLNFVQPYFAARGFFDLLKGPSFVGDLAVARDGFTAGAEVGYDLTSAVITKYAAALGYTAGVYSLTVGADKNQVFTGSFYQRVSPVLEVGAKSTYASLTNANAIEFATKYDLDATSSVKAKIQDSGIVDLAYKQDLRKGVTLGVGASFNALKLEEPVHKLGWSLSFSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV32</accession>
    <name>A0A1E5RV32_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Trafficking protein particle complex III-specific subunit 85</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g398</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RV32">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV32"/>
    <dbReference type="InParanoid" id="A0A1E5RV32"/>
    <dbReference type="OrthoDB" id="203724at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990072">
        <property type="term" value="C:TRAPPIII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024420">
        <property type="entry name" value="TRAPP_III_complex_Trs85"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12975:SF6">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12975">
        <property type="entry name" value="TRANSPORT PROTEIN TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12739">
        <property type="entry name" value="TRAPPC-Trs85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ90792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="599" mass="69180" checksum="A4F879F0B11F9976" modified="2017-01-18" version="1">MATAALTYDKDSIKNSILNSVAPVVTVSSSPELNERLQRTLNIQNLQEYMVKYGNIETTASVLXENASGIKLQDLQGPFSLRFAADMETLLXMPRDSPDNLQSRIQSYLSTAEQSQDIPMQDSKEIFQYFFETSMSSYDLSVPLTMFNHPVVCMLGFDLNGSQENEDKYERAREMLVRFKNTKKTKKNEATKNWKYININDILPVFVMCYNDHDQKSFEDACALAKQLKKQLFVESFLLPIWHTTDDENKVSQQDVANLQTLLVQMKVNTIKPFMVRKIQFWNETILNHRKSLSKLFFRKFSRQSSPALSPSDGSNATLETAEEEMLLRKLGDWSFMLKDFKTAFSVYNQLVKDFNATVIQEILPTNVNPQLLLASSLFWSAISIFMGAQTILTGKMIKTELDPLITRGISEATITETLEDGLSDSANMFIQVFKFLLPLSELFLTLSDEWLGVPYAIRWYEEILYWSNNKYGLIECFIWERLSLCFKLRVDPRVIHHIKLAQCKNSTDEVTEESEPEPQALINPNKLDWGKDYIMQGHQRQRKAVFFELLAARKWLECNHVNQTKRLILDIDKRYAGLPFAHRNELLLFKLKEKTGLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVF4</accession>
    <name>A0A1E5RVF4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein GIR2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g317</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVF4"/>
    <dbReference type="FunCoup" id="A0A1E5RVF4">
        <property type="interactions" value="784"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVF4"/>
    <dbReference type="InParanoid" id="A0A1E5RVF4"/>
    <dbReference type="OrthoDB" id="277175at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040213">
        <property type="entry name" value="GIR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12292">
        <property type="entry name" value="RWD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RWD" evidence="2">
        <location>
            <begin position="9"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ90922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="290" mass="33713" checksum="9B0F137196CDFA8E" modified="2017-01-18" version="1">MDYKEEQTQELEVLESIYPDELEITKSEYPKIEFLIKKLPLEFIPKDSSSFTTDSISQNHFLNISFKYPEQYPDILPEVKVEAVKLAKKSRRNRAHGNDDDDEYDDEEDDDEDEDEDGDDDNDDDDDDDDEIQYDDHGNPIASKLADLPSQVELQNSEFLNTLINEKLPQQLEEDMLIGIPMIFNIVTILKEECEQYLTETLATKELEDEIKMKAKEAEEQKKFQGTEVTPETFKAWRLKFRKEMGIDVRDGKRKEDLHKNKLSGRQIFEQGLAGADDDENTEDALPTEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVT4</accession>
    <name>A0A1E5RVT4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Guanine nucleotide exchange factor subunit RGP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g351</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVT4">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVT4"/>
    <dbReference type="InParanoid" id="A0A1E5RVT4"/>
    <dbReference type="OrthoDB" id="1918at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014848">
        <property type="entry name" value="Rgp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12507">
        <property type="entry name" value="REDUCED GROWTH PHENOTYPE 1 RGP1, YEAST -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08737">
        <property type="entry name" value="Rgp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ91004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="599" mass="68887" checksum="00F700D49771705E" modified="2017-01-18" version="1">MTFDHRIHHELLSDNIRLEVRLETSPIYSGDYCQLLLRLRHLGSEDELKRLEQLIKENDCKLQDLKNTYSERLFSVGLHEKIDDIKKLNSNRFFSFLKSENPQDREIQTQWNSLQSERAQLQGDLDFHKPVQMVSGELHMTGIFKFSKLAIDSTKLEKASKLPSLFYAEDYMPKKEGTLSETDIIPFFLVPQSLIFTEKTLTAGSVCSFKIKSQTPLPTDLPASYIAISNNFSIAYHLCLDIFALDNQTGLPLQFPLKIPLFVYPFFDDEGKQKVCKLDGEIRITEPFVVKKIIDREEEHLSLLHSNESIKPSHPNGKLENNNEGISSPSHSHXDEESVNMQQRKAVGLLKAXFKELADQIDPTSYDEDELISKMLKKQKDKTLAESFSSKLNITANDTKMNVANIKNHSKNFQKVVPYLNSLENLTYKQEYMINRNAENIVKITLNKLVYQTSDDINLSLDFARSEKFKVTSVLGVFESHEIINPHYSILENDSSSKDKKQGFPIYENHSTMFDETTSLSMRFIPYSFNNFNIIPSLFKNNIFQHKYLIKIKLVLVDKSEHFLVPGTKEDKYGKSWKAASTLDGESIQFYIPVLVLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVV1</accession>
    <name>A0A1E5RVV1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g563</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVV1"/>
    <dbReference type="InParanoid" id="A0A1E5RVV1"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ90959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="232" mass="25059" checksum="E5CD89B5B52C3138" modified="2017-01-18" version="1">MQNTFEQGPCAAIVRVAKNANTTSSKMKGDARAKTAKISNHNMHNEGEKLVANSTPFVCTRIPALDARLTVKTSALASGLPSSAHAQALSWWPESYSKSDECETMEETLAACASRTETLKAQSVVYIGRDISFVGSATTGTRASNFKICDSKLLPYRANYANCTKTSLFFSFFFHGSGTDRTHNHLDITNCGSGSDKHNGKKCAVDHGIDEAEVEAHENTQTLVTVKMGISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVV8</accession>
    <name>A0A1E5RVV8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g557</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVV8"/>
    <dbReference type="InParanoid" id="A0A1E5RVV8"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="431"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="519"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="440"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="695" mass="76052" checksum="CE5978CE597E1DAD" modified="2017-01-18" version="1" fragment="single">MSITANQHEMQSNKSQNAIPQTNSSQIPNTEWNCRNTLRNAFNTIKQHFIENRASFFLNKDGLPKEDVLENANAAENFASRPMAIEANSWSPQLTQSSSRITQSNLRIAQLSSSFTPITSGIASQGAFKFAFKSAFRWNYVSLGLANAALGSEYASAGATKGIRNFTETRSQGGQFSSSSSPILQMIEKERAATKSKRARMRGAKAALKRKLHKSKAALKCKLHKSKSELKCKLHKSKTELKFKFADRGLFKACGKSAQAYEFACKRVQPAESLRVGDSLESRGFNTNDANPNSETALPVSSTSASDYGAVPNDGFSEQETLGNKKAVKRSKRARIRDAKAAFKCNVQHSKARFKCKLSKCSFLKVNKDHYQANEDSNEKGRPDELLREIDTLTSNGVDIDSTEEAAGLAMLDNSTSDASPDTPSTNILGISAQGSPSNTQETQATTLATEDKTSEGELVQQIIKKVRFILPERNYSDDKKTLAHAYSFASLGGSNTTSNSNFAPCSIDYTVEQELEQDAADDDAADDEQDAADDDEADDEQNAADDDEQNVADDDEQNAADDDAADNEEHEICEAIKAGSYSVSRENGYSGSKKTLAHTSNITSLGGFNTTSIPNFAPCSIDYSVEQKLEEDTDNDEEGVDNDADDYEFYEAIKARRYSISRENDYSGSKKTLAHSFAPRSIGYSVGQELEDDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVW4</accession>
    <name>A0A1E5RVW4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">DNA repair protein RAD4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g425</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVW4">
        <property type="interactions" value="153"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVW4"/>
    <dbReference type="InParanoid" id="A0A1E5RVW4"/>
    <dbReference type="OrthoDB" id="300780at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000111">
        <property type="term" value="C:nucleotide-excision repair factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071942">
        <property type="term" value="C:XPC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.20.110">
        <property type="entry name" value="Rad4, beta-hairpin domain BHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.290">
        <property type="entry name" value="Rad4, beta-hairpin domain BHD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2460">
        <property type="entry name" value="Rad4, beta-hairpin domain BHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.260.10">
        <property type="entry name" value="Transglutaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018327">
        <property type="entry name" value="BHD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004583">
        <property type="entry name" value="DNA_repair_Rad4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018325">
        <property type="entry name" value="Rad4/PNGase_transGLS-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018326">
        <property type="entry name" value="Rad4_beta-hairpin_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018328">
        <property type="entry name" value="Rad4_beta-hairpin_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042488">
        <property type="entry name" value="Rad4_BHD3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036985">
        <property type="entry name" value="Transglutaminase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12135:SF0">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-C CELLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12135">
        <property type="entry name" value="DNA REPAIR PROTEIN XP-C / RAD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10403">
        <property type="entry name" value="BHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10404">
        <property type="entry name" value="BHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10405">
        <property type="entry name" value="BHD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03835">
        <property type="entry name" value="Rad4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01030">
        <property type="entry name" value="BHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01031">
        <property type="entry name" value="BHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01032">
        <property type="entry name" value="BHD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad4 beta-hairpin" evidence="7">
        <location>
            <begin position="480"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Rad4 beta-hairpin" evidence="8">
        <location>
            <begin position="539"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="Rad4 beta-hairpin" evidence="9">
        <location>
            <begin position="612"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="17"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="777"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="76"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="801"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="824"/>
            <end position="833"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ91034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="851" mass="98110" checksum="9CEE997A4D05F836" modified="2017-01-18" version="1">MSKRLSKEYFDLVKDALAEEGDSRTTRQSKRKRPNEPKPKVDEIVNLDSDSDAESTKLNSQDVIDLVSTDDRAPSSEDDFDDEEFNSDDFEDVSMNEHASSGEEQDDNMTFTINTTKQKPETNKKKELNSVSKDEKRKRLLFHETHLLTLLVHGYQRNKILNDPKLHKKLDKLIPDQVFDLLHPPKDEELPLRSTRKLLDGLKKCIEIWQKYYTCSYDNNHYLMGLYMRPWNEIGHKSSKKRAPPILQEEFLTXLLKKKTGSREIGTQGFVALLRAVGLNARLVFSLQPPDLTIMKKDLDNKDDFDLQLLLKQTSVFIPDKKLRKMRVNTDAKYEVQTKPENDIAILPVESLKYPLFWCEVWNKFNKTWITVDPMNFKTIEQHTMNLNSRSKLEPLSHRQANKFNLLRYVIGYDRKLGCKDITPRYTTQFHSKVYKKRCTRDXAVKEWYEKSVNYLNSYGGRGVLKIDEYEDEFFANKLNLETIPDSLQDFSGHPKFILENQIPSDSVLKDNAKHCGFFRIKNRNKSLKVYLKSDVLKLRTARQWYMEGRVLKAGAQHLKIVKKRKFRPTKXYGDMNXGGXNDDDDDDMERLYSFDSTDLFVPPLASPKGEIQTNAYGNIDVYQPNMIPMNCVLVEDPLAIRAAKYIRVPFAKAVTGFEFEKGFTVKPKISGVVCLRDFKDAVCFSIEGIKIAEEEEKEENKEVAALNHWVTLLKKLKIQQRLNMLHGSVDAENQSLKEAFDGGFVAADRNDEQDFDQEEEAGFLQEEEAGFLVDEVDDKDDEKEASDTTXRFVQGSLAENKLEITHGDDQDGFLTDSTGAVGDAKKDEKDDNGANLSDEYNDFLKELDXD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZJ5</accession>
    <name>A0A1E5RZJ5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Guanine nucleotide-binding protein subunit beta 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZJ5"/>
    <dbReference type="FunCoup" id="A0A1E5RZJ5">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZJ5"/>
    <dbReference type="InParanoid" id="A0A1E5RZJ5"/>
    <dbReference type="OrthoDB" id="10251809at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244:SF471">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT BETA 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244">
        <property type="entry name" value="KELCH REPEAT DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="580"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="584"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="867" mass="97977" checksum="0575DCDB194B8CE3" modified="2017-01-18" version="1">MNINHFQNDYKPLDYVASYISGYELLNPSSLETEKKQQLRNLEKYYLGKNAEKEGSSSNSFQSLISQNSMISQNSMISQNSQNSMVSQNSQNSMLSHNSQTEDSFRKELIVTKLSKQHNIVSHLKADTRQQLYYHALDPQSKEIVAKELSSYLNYSNNLRVTTDIFSHQIRKQNIWIPNLQIESKFIAQQKFSPDQRLQSCSDPDQIFKPLSKDIFNGVSYYPKLLGETKIPGLVYHNSVEINNTVYIMGGLRSNFENTQCIDDISNFYVDGLDDLPAPLNKDVINNPNMVGNKRFYSFNTISNSFREYVPQGDVPSALLCCKGSPISERYIFYYGGFEIITEMINYQKDLKTNKTLYFLKSRALINNTGYILDVATFTYTKVELVARPSKYFSYPTTVPRFGHSQTVVMKSKNEDKFSLSCTNTQDDNIILNVKQFPDNIMIMIFGGYKQVGDSKYEPMNDLWSVELPVLEKGVKNYMRFTDTAFATMHKNSLMNTTLSAPSTAGNTPIVSQTSIPTINSPASNTNNTTAFTNIQEWPCPRGFQSATIVQKSHFEKKTVKDTIVKNLIDNYKVKSTRRRSSNHYHNFNLRSGSSHGPAQSDGSAISISSFKAPNTSNNSSHPSLNEPFQVRKKYVQNMSVIDDSDMELLLPVSSSSVSFDEDNEQLDVDSCFLVIHGGTFNEETLGDFWCFDFSTETWEQKFLQASNINAPATYKHINLDLCGHEMFSSGEYTFLMGGMSAACKNYKRENYSLLTLLHLPTLRVLNVQQVDTVEVEEQDIRILGSPVRASNGVYYMLGGVFMHIFKKDMIDPAVLPNTEEHPVGNGNNPASANTVISNHTVTQHYLHGAILASLAPTVSVRSQTFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZK4</accession>
    <name>A0A1E5RZK4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">20S rRNA accumulation protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g85</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZK4"/>
    <dbReference type="FunCoup" id="A0A1E5RZK4">
        <property type="interactions" value="348"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZK4"/>
    <dbReference type="InParanoid" id="A0A1E5RZK4"/>
    <dbReference type="OrthoDB" id="443682at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007320">
        <property type="entry name" value="PDCD2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47524">
        <property type="entry name" value="20S RRNA ACCUMULATION PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47524:SF1">
        <property type="entry name" value="20S RRNA ACCUMULATION PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04194">
        <property type="entry name" value="PDCD2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Programmed cell death protein 2 C-terminal" evidence="2">
        <location>
            <begin position="325"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ92397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="444" mass="50066" checksum="1EAFBE5867297173" modified="2017-01-18" version="1">MMSQEELKIKELKISDDASHEESDYDDDDESIYEKGRTEVYLGLVDANIKGTDEVTVEDTFIGGDPVWLHPDSEPNCTLLKCKSCAQSMKLLMQAFAPLDKQQVEEITGKSSHLKLTTTQYIDSNDDRVLYIFVCNKCNRKEGSIRCIRGVKKANSLKTTKLDLSENDNHKQRSVLGDGFQNPFVFSTSSSAPNPFDKEATTSNPFGSAASNPFETTTIEKPSSTPQSLEQLSQKNQPSAKTLRKLHDQLENKKFSGFPGYFVYVEAESFKNKTPDHLKMPENIRIDESAISGEDDYIQSVTNGNASKNVTTDPRTEKLSKILDDDVFQKFQEIVGYNPGQVLRYDLGGDPLLYCQDGLSNKKLKDYARTVASPTYNPGSARVFEMQLMPKLIIDLEESISLKEGMEWGTIMVFTDIENYIPHYDEHGVGYVEELCKVQWEPRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZR3</accession>
    <name>A0A1E5RZR3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ectonucleotide pyrophosphatase/phosphodiesterase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g105</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZR3"/>
    <dbReference type="FunCoup" id="A0A1E5RZR3">
        <property type="interactions" value="225"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZR3"/>
    <dbReference type="InParanoid" id="A0A1E5RZR3"/>
    <dbReference type="OrthoDB" id="415411at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047429">
        <property type="term" value="F:nucleoside triphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009141">
        <property type="term" value="P:nucleoside triphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF120">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="922"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ92480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1013" mass="114398" checksum="1A4EA4935E971D84" modified="2017-01-18" version="1">MDTNNNSARAHFSDDDGEHMFDLENDDFMFENDDHTFSLNSSSRTTPAINHNNNNKSQQDHQYLLKINKFFQNLNSTLFHPTALFKDSRKTVSDKIQKSLKHGIELYNMDNHLHDDDHEHADDHAGDRDGLNDLQNMLSFHTKLRKRRFFQKTVLRLVGFTLVLLLFLYLLLNGLGVVSPSRFGNNKYASGRGGKGKNKDSSGDGNHKDFSPYRLYNNGTVNMYPVTLLLNLGGVSPMFLNSMDTPILDKIYRRDFDNDLAESMLENNSTGSSLDEVFTQTGVLSTPYMIPGSSFQTIPNLWSLITGLDPEYHKIYNNDFDLFFSNKLSDDEKDAVKSASEEATNQANEAFQKGLARYSFNGFDVTSQEKEFKSHTKNPQKNLYHLDQIYNGNHSIWAQIQKSYFNKLDGMFDFKCDVLNWPGSLVDYAYESNFTKPFYLQQNSPAKSTTKYDDQEYLFSSLASDIKKKNKKVSQFDVLKTVNYIFQKIDDAAAAAASDGSVGKSEDVPGLIIQDIPVMGEQGFYDDPLYYTERLQMMDKYIGLLLRGVAQRNMTHMFNLVVTSDHGTATVPTENIHTWEEYIYRDKELGQEVERLLSSSSYSYPSSSSFPLFDEETDNSVGLVVFHSPYLGFTHPGLNLEQWKNFESGMEGHPYGVSGSSVKLTGADAESKEDLKKKNFYQLYSLLKDVFANSSFHVLQNHNLPANLKYLDKMHSGQDTLWVIPEYNHYLIPPNYKGLSKTANAYDDSANIFQNFNLKKFNSETDTFSKTLFVNGYDCLNGSVNELSIINDQLEMSSLFLGVGPYFQNFENYDLKPFKNVEVYQILSDMIGLRNYNKNNNGTFAYPLSLGEANLWLWESSEEMSQTDATSWNNLREKFGARGENSFLKKWVGAEYLEWRAKNVAIEEVGAVSSATTKTSSSVFSNAMGESTSSSISGSQTSSIVSSSSTSFFTTTSISSSMSSRTSTGPKPTSAHAQNLNEVFQEIVSEVKEEAHELIDKVEEMLAGTASVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZV5</accession>
    <name>A0A1E5RZV5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">D-arabinitol 2-dehydrogenase, ribulose-forming</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g106</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZV5"/>
    <dbReference type="FunCoup" id="A0A1E5RZV5">
        <property type="interactions" value="237"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZV5"/>
    <dbReference type="InParanoid" id="A0A1E5RZV5"/>
    <dbReference type="OrthoDB" id="5325318at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF115">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE FABG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ92481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="323" mass="35627" checksum="4FA9E9EEE978D882" modified="2017-01-18" version="1">MATPQPHTSASTESMVKSATPILPQLPKIEDLIPSFNLENHVTLITGGSGGLAHTLAQALILQGSSIALMDLNQEGLKNLKKELENFIKHYHDKIPNADKIVISIWQCDVSDPKHVDEVINAIPQKHNGVIPMKLVHTAGYCENLKAEQYDPIRFQKLVNVNLNGSFYIVQSLTNLHLKTKEAILNNNVSSLALPNFNMKLSYVLIASMSGLIVNHPQPQSAYNASKSGVIHLCKSLACEWAKYGIKINCISPGYIATELTKKVISQSVEGLELMREWTSRVPLERMAEPKEFIGSVLYLLSDNASSYTTGENMVVDGGYTCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZY6</accession>
    <name>A0A1E5RZY6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Aromatic amino acid aminotransferase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g34</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZY6">
        <property type="interactions" value="172"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZY6"/>
    <dbReference type="InParanoid" id="A0A1E5RZY6"/>
    <dbReference type="OrthoDB" id="691673at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047536">
        <property type="term" value="F:2-aminoadipate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008793">
        <property type="term" value="F:aromatic-amino-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009074">
        <property type="term" value="P:aromatic amino acid family catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050859">
        <property type="entry name" value="Class-I_PLP-dep_aminotransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790:SF2">
        <property type="entry name" value="AROMATIC AMINO ACID AMINOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="7">
        <location>
            <begin position="235"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="142"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="154"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ92315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="665" mass="75037" checksum="DB4EF01470FA0A78" modified="2017-01-18" version="1">MTXTGINSSFNHSGSTCSVDXXHTNTSNNSNFSKRKPVIDFAAKYSKHLSKHDHLKVLFPFWEQENLPANLKQKSDMIALGSGTPNEVLFPFEKIQISLKQSPRDTETSVVTSITMNDETHGKGGVCVTPTTSNSLAYALGREPGSLDSDLKQTTTTXTTTTTTTTSSSSTTXPELXGSSXASSVVSSSXFECDDNIDSSESDEDFEHLSKHPRRLSIRECLQYGDTCFGNFKTLDMIKSLLIKFKNIPPYANWDYLVTSGTSTSCFKICQLLVDEDTTVLVEEFTYSVILSNIKFANGNWIPLKLQFDTENVDNQGIDVEYFTELLENWEIKNPNKQKPKLLYTIPTGQNPTGMTVSMEKRKRIYELCCKHDIIIMEDDPYGYLKFPKYDKENPTYNPYKSSSSESSAEWTIXHYINKTLMPSYITLDIEGRVLRCETFSKICAPGLRLGFVVGNQYLIKKLMNVIDVSTREPSGLSQGALLNGMIAMDEKYSATLKKNALMSGNWKNHNQVAKTMSPLDGWAHWCMNIAGEYLHRRNLVLTELYSSKAFHENQFSVLEPSAGMFASVVINLKPQWKKGLEIKKAMDYLNYCLLEEGCDCILGYKMTVDEKLSLERSNFLRITFAQASNDQVLIEACKRIAKAVTRLFDEYQTDKQKFRVLEYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S070</accession>
    <name>A0A1E5S070_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycogen [starch] synthase</fullName>
            <ecNumber evidence="9">2.4.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g67</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Glycogen synthase participates in the glycogen biosynthetic process along with glycogenin and glycogen branching enzyme. Extends the primer composed of a few glucose units formed by glycogenin by adding new glucose units to it. In this context, glycogen synthase transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;4)-alpha-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18549"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9587"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.11"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:18550"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyltransferase 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.11" evidence="9"/>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5S070"/>
    <dbReference type="FunCoup" id="A0A1E5S070">
        <property type="interactions" value="502"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S070"/>
    <dbReference type="InParanoid" id="A0A1E5S070"/>
    <dbReference type="OrthoDB" id="6335297at2759"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004373">
        <property type="term" value="F:alpha-1,4-glucan glucosyltransferase (UDP-glucose donor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000045">
        <property type="entry name" value="Glycogen [starch] synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000177">
        <property type="entry name" value="Glycogen [starch] synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008631">
        <property type="entry name" value="Glycogen_synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10176:SF3">
        <property type="entry name" value="GLYCOGEN [STARCH] SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10176">
        <property type="entry name" value="GLYCOGEN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05693">
        <property type="entry name" value="Glycogen_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="6 9" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="4 9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ92514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="720" mass="81975" checksum="2AC3ED08036F7599" modified="2017-01-18" version="1">MTRDIADHLLFETSTETANKVGGIYSVLKSKAPVTIKQYGPYNYHLLGPLNRNTYEMEVDVLPFDDVDTEGDIDTDSDSATPYEITTIKRALLKMQERGVKIVYGKWLIDGSPRVILFDIDDPQLNAQFLNEWKKDLWEKCGIPSPENDFETNRAIMLGYLVSWFLGELCYINKKNAIVAHFHEWLAAVGCPVLRARKIDCCTIFTTHATLLGRYLCAGKDKDGNDIDFYNNLQFFDCDEEAGKRGIYHRYCIERAAAHTCDVFTTVSDITALEAEHLLKRKPDGILPNGLNVTKFQAVHEFQNLHLKNKLKINDFVKGHFLGHLDTIDFNNILYIFIAGRYEFKNKGVDMFIESLARLNYKLQCAGSKITVVAFIIMPAENNSYNLQTLQNQAYVRSLEAHIKMVCTNLEAQLLDYALVKHSVPQELSSILSTTDQISLKKKIFKISPSKTILPSITTHNIPSPNDPILSTIRKVKLFNNDYDRVKIIFHPEFLNSQNPILPLEYEEFVRGCHLGVFPSYYEPWGYTPAECTVMGIPSITTNLSGFGAYMEDLIDKEQSSMYGIYVVDRRMKNGEESIEQLVDYMEDFCSKTTRQRINQRNRCERLSDLLDWKRMGLEYVKARNLALKRAYPDVFAQYLQADDQYGTGTPEIERVYKVGKPFSVPGSPRASTNGSQTVLMTPGDLGTIQDANYVDDYFHLSMGGEEEAESSKQESDGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S086</accession>
    <name>A0A1E5S086_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Multidrug resistance regulator 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g149</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S086"/>
    <dbReference type="InParanoid" id="A0A1E5S086"/>
    <dbReference type="OrthoDB" id="2943660at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF12">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="37"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="557"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1334"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1371"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1618"/>
            <end position="1648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="557"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="567"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="595"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="636"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1371"/>
            <end position="1389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1390"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1639"/>
            <end position="1648"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="9">
        <location>
            <position position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ92383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1713" mass="192856" checksum="13178EF07B581BD3" modified="2017-01-18" version="1">MSKVQAKKASASKKAAAFKSKDTLDDGSVKKRAHGTSCLICRKRKIKCDQTKPVCLACVKHDCSMDCSYSNEIKKPVTKSTSSASAMFIANENETHELGNKHYGLPNRKASPVSLDPSASSKVMSGAHDIDQNLLDGKPTILFSRGESTKGSQSESLMQLLNKQSSEISLQNRLKDAQHDHATMSPLSNATYSKLSSKIRKTVRKQKMIKEFSEFKKKLEGLEALLEGSIKEEEFEIEHCSPASDASLSPYVTNSLAASDYSELKFIPKMSFYDGFEQVNVLSARLLFLGPLSSSTIVKKDSFCIVLSAMINKTREDIAKALEVQTEEKYQGLRVTARHMAEQVNIHIKSDSKIVNENIDNGVPENAELSGPAEGQQSLQXISSLVESQLQMPISEDYPNYGITGSNDTIAEQDETRLKKKNGFSAMVNDETKKGQEHFGFKSPNIEASNINKSERAHSMSYNSGPDAGSLDFFKKRSYSSNIQNKNHLKYNKKSKSSISHLISPAAGDDEDDEAEEVFEQKLLENEGLDEMDTILQEGKIANNPLSVDNLLNKKIGSSSNSRSGSTTHLSPSNPMLVSSNTSDIRSEKLPPRNMHSNLPNASWKGSLQSSLESPRAQVVSLDESQGLSGXHSLDNGATKSNDTGLNSVSKASAHSYTDEQPTLXNLKAQALPQADLKERFFETPQPXMLQASRNAEDDINMWRALDNVLTKIQKQNSRPQERIPEGLFGQSNDVKGKQELDVLMRIRSILPSAKLVRMHIDNYFKSPIHGLYPILNEDWFRDTMQTVVSGLDKNSDQQPRINATRRFDLSKLGSLLVLLRLSYLQCPETLEQCQTDAERFLLSHPIGPEFIDTAQMCLNLFKMLRKGILPVLHCALLLRVYRKYAPEDGDIVDGTDSETFTGLLVRSSTSIGLNTDVGRSHWVQEQPMYIDQWRKCWYVVYFFDFMEALNMGNTLSIDVDTFDTKLPEFKLDPHTGVNPTYILDPQLEDVVVGNMQRDFQMTLICRELLCAITNKRQKFSILELKSSIDRLDRFIRKEYGADMKSIIEMPADTLPQTVAKVNCFKNFVDINSLIFMVYLHIFIHIDKNIVSNLNAIKSDHSIEENMQLFHYYLKKVISLYIEVEPALILCYESKKKNSNKVEEIFGKSTKLLVIQSCEYVLTRFRQGLYVVISRTLHFHYNFLCNIEETSMEQANPERKEIAAMVSEIISCAMDKLEYSDSICRLLSQKYFQIWRISKHSQFMFQLLSNMENNLFDKASKINNDHRNECNNTRFAIYPFKYIPHINLFSHYKLKHFQEIFSTLSSVKWNVFSSFLTPQELTEAKDYRRHDRKAFIKNTRKSKKNGSTGKPVTGGLEKNVKKKIFKNAIKKDESHSADKSKELGTENEKNFSPNMHSSHSSNTPVVGQIDIPIDEIDKFWYSTVINNSNVFAQMPNELANSGVPELNVTNGESFHFDDASMKHLQSNLQVAANVHPSLSHEAQSRAAHPPTEFFDKQDHLKNASLGQRKASTSIATPFDPNLGNNEELDEIFNNKDMLKAHVFQKLQELNAQAEMPSLESSMAYSGNRAFRNGADLRHTTNDAGAELMQNSHLSETDTSHLNINTASNLHNTVYRENTRTDHGTSHPANGLHAADSTRGHNANETSSGYIQPINTNHANFLANDADMNTASGHKDGGMQVFDNIADILKMQEMFNTDFLFPGSNSYYSDEINF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S0D4</accession>
    <name>A0A1E5S0D4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g165</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5S0D4"/>
    <dbReference type="FunCoup" id="A0A1E5S0D4">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S0D4"/>
    <dbReference type="InParanoid" id="A0A1E5S0D4"/>
    <dbReference type="OrthoDB" id="289247at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000309">
        <property type="entry name" value="Chromosome 21, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040250">
        <property type="entry name" value="Nucleobindin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19237">
        <property type="entry name" value="NUCLEOBINDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19237:SF20">
        <property type="entry name" value="NUCLEOBINDIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184965" description="EF-hand domain-containing protein" evidence="3">
        <location>
            <begin position="19"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="127"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ92433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="254" mass="28934" checksum="805708EBC05B52FB" modified="2017-01-18" version="1">MKLAYLSVLPFLSSLAHGAIIEEDANDNAASSSGSTKNTNAMIDETINVDVESPPEGMEWQDWHMLQEHQLESYSPETFFALHDISKKGYFDSKDILTMYGLNRDEVIGSGDGMGKHDESEAVDPELADRVVKFIFKLFDIDDNDKITKQEFLNIAERGTKFPDLGVGVGHHGDFELEYEFHHWNEYHKDEDPDVKNVHREDIEHDLLHHEHEIEHEENVPRGGSRATVITDDELEARIKSKNIPAAFKANVYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S0F9</accession>
    <name>A0A1E5S0F9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">1,4-alpha-glucan-branching enzyme</fullName>
            <ecNumber evidence="4">2.4.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glycogen-branching enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g78</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Glycogen-branching enzyme participates in the glycogen biosynthetic process along with glycogenin and glycogen synthase. Generates alpha-1,6-glucosidic branches from alpha-1,4-linked glucose chains, to increase solubility of the glycogen polymer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.</text>
            <dbReference type="EC" id="2.4.1.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.18" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5S0F9">
        <property type="interactions" value="430"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S0F9"/>
    <dbReference type="InParanoid" id="A0A1E5S0F9"/>
    <dbReference type="OrthoDB" id="196493at2759"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003844">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11321">
        <property type="entry name" value="AmyAc_bac_euk_BE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02854">
        <property type="entry name" value="E_set_GBE_euk_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000001">
        <property type="entry name" value="1,4-alpha-glucan branching enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000003">
        <property type="entry name" value="1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006048">
        <property type="entry name" value="A-amylase/branching_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037439">
        <property type="entry name" value="Branching_enzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651:SF3">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02806">
        <property type="entry name" value="Alpha-amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000463">
        <property type="entry name" value="GlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="12">
        <location>
            <begin position="159"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="350"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000463-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ92495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="698" mass="80638" checksum="C652DB18CAA2133F" modified="2017-01-18" version="1">MNENVKRELMEIDSYLEPHFSVLNERKNWADNYLNKLTNNGEISLVDFARNGYKEMGFTINKNEKNIYFKEWAPNAHQAFLIGXFNNWNESSHELTKDEYGNFSINIPALAEKNDFAIEHNSKIKLLFLLGNGEKIYRLPTRSERCTAPSEQDAMTFGHTYEARFWNPKTTYQFKHKRPKVDTQVEGLKIYEAHIGISTPEPKIGSYNEFTENVLPRIAKLGYNVIQLMAIMEHAYYGSFGYQVTNFFSVSSRYGTADELKRLIDTAHSLGIKVLLDVVHSHASKNVDDGLNMFDGSDYQYFHSIESGKGEHPLWDSRLFDYSKYETLKFLLSNLAYYIDVYQFDGFRFDGVTSMLYSHHGNLFGFSGDYNEYLSRDRSFVDYDALSYLMIANDLVHQILPGHLGITIAEDVSGYPTLCLPRHLCGVGFDYRLAMAIPDMWIKIIKELQDEQWNMGNIVHTLTNRRHAEKVVGYAESHDQALVGDKTLAFWLMDAAMYTDMSLLKPLTPIIDRGIALHKLIRLITHAIGGEAYLNFEGNEFGHPEWLDFPTEXNGQSYYHARRQYNLVDDGLLRYKFLNNFDSAMQHLEHQYHWLKSAQAFVSLKHETDKVIAFERNGLLFVFNFHPTNSYKDYRVGVEAPGQYKIVLNSDNGLFGGHDRIDDVNGEFFTTDMPWNNRANFLQVYIPCRTAIVLAKFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R098</accession>
    <name>A0A1E5R098_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g4459</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R098">
        <property type="interactions" value="859"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R098"/>
    <dbReference type="InParanoid" id="A0A1E5R098"/>
    <dbReference type="OrthoDB" id="10020333at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14019">
        <property type="entry name" value="STKc_Cdc7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF23">
        <property type="entry name" value="CDC7 KINASE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="191"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="53"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="114"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="381"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="136"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="393"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="648" mass="73336" checksum="AB93A5DC2064DEC3" modified="2017-01-18" version="1">MDESVFITDERSVKTSGNVKEENALLQTRKHGFGYMNQSNEASLVEVANEVQITDSGSXNSNEQPEVPQHAKEPVHKKMRFETPKQEIKTDQVSSVITSPLKISPTKIISKQDLINSPSKKSQAVRHYRSLTTQEDATDDEEENSEDGGQAEKEALSSDDNQSSFDPFVPQDIIDEMQDLLHDFPGLKSKYQLLDKIGEGTFSSVYKARDKTNRLNATKASLKNHFWAGSITGSAKKPTTYVALKRIYVTSSPQRIYNELKLLYILSKSPRIAPLYDALRYQDQIIAVLPYYAHEDFRVFYRVLPIKGIKKYMFEMFEALAYIHERGVMHRDIKPTNFLYNTKLGKGVLVDFGLAELENSSDHSVSEGSIDKCPCVNYMEESTGTGPESSIPDGRKLRNDHKHGDGGNKENVANTXKVFGNNNAIDLTRGYPKHESRRSRRANRAGTRGFRAPEVLLKCSRQTVKIDMWSCGVILLCFLARRFPMFQSLDDVESLMELSVIFGIKQLNKCCRLHGLGCNISTALLQGISEEGFKNGLKGYVKKLLQDENKMGTLPAYSPAWDTLSYLEGTLLEENXSAQHGDSSXSNDLSNEGKNEALDHYKKSLFEDHKNCFEILQMCLTWNYHKRPSAKDILKCKWFNELTDDYEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0D5</accession>
    <name>A0A1E5R0D5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Phosphate metabolism protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4511</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0D5"/>
    <dbReference type="FunCoup" id="A0A1E5R0D5">
        <property type="interactions" value="135"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R0D5"/>
    <dbReference type="OrthoDB" id="1076608at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022257">
        <property type="entry name" value="PHM7_ext"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF139">
        <property type="entry name" value="PHOSPHATE METABOLISM PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12621">
        <property type="entry name" value="PHM7_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="436"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="481"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="574"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="620"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="649"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="687"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="715"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="11">
        <location>
            <begin position="14"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="12">
        <location>
            <begin position="192"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="9">
        <location>
            <begin position="434"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="domain" description="10TM putative phosphate transporter extracellular tail" evidence="10">
        <location>
            <begin position="974"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="840"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1054"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="840"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="851"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="895"/>
            <end position="905"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ80365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1076" mass="122339" checksum="4CA8B0FD7576ABCA" modified="2017-01-18" version="1">MSTTDTSASSSTSAFVTSLIFNGIVAGVFVILFIKLRPREKRVYEPRVNDPQMDIKTLKTSEKVEEPIPKGYFEWVPFLISKPHSFLIQHCSIDGYFFLRYLSIFGAVSLLGCFILFPILLPVNATNGYNLKGFELLGMSNVVNHKRFYAHVFLSWAYFGMIIYIVYRELYYYVTMKHAIETTPYFKSLVSSRTVIITEVDMIKIDKDILYNKFDNIEKIVFGKNFEDLDKCCDEREKYANKLEKLLNNLVTKCVTKRNKWEKKNQFPEKLNGKPENDFQSYTGKRPTQTLGKSPITIPLVSGKQVDTIEYYFHNLEKLNKKISKYQQNWETNFDSVGAVFITFKDQYNAQKCFQSIDYIFGESVLSGVHPLHNGEHGIKGHATGAIKAAGNLAQNTMKQMFKKPFNDKYIGVSPDDLVWKNLGMDMKKRKAKKTLTNFVLTMMIIFWAIPVAVVGCISNINYLTSIVPFLNFINNCPKVILGLITGLLPTILLALLMSFVPVFIKMAGKFSGLLTLQEIDNYCQKWYYAFQVIEVFIVVTAASSASATVKAIIKKPSLAMTLLAQNLPKASNFYIVNFLLQGLSFPSGILLQLVPLILSKVLGKLLDKTPRQKWARWNTLAAPSWGVLYPVMELFVVIMLVYSIIAPIILVFSTICLTLMYIAYIYTLNYVMAFAFQSKGKNYVRALFNIFCALYLAEICLLGLFIMGKNWGCLVLEVVIMVVTVLSHLYLQQRFLPLLDIVPLSILRMNGVPVDPFDAIDPDVYAKYPHQDQGWKDYKDLEDGLEEEQENEVTEIEQGHQKIRPATDEELRRAHYLSEDADQGPEDVFDDSNELSATQTKTTNANGEPSNTHKNSLTSTLTSNSLQRKSTFVEEDIKKHRGSQDFNSNTAAANGESLSNINSESSEDARLRFHKLHYADIQNLEPPPINEVGGASSRIAQHNMRVNVSEYPDNFANTESLNRHGIKKVLYNFFHPSSSYNFVEVRERLPKVFDLINEDGMPNPADKDNSPYKYPRLDEKPPIIWIAKDPMGVSDNQVAYSGSFNVDVRNDYTGFNGQGKPYYTGDPPDFEKRDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0F6</accession>
    <name>A0A1E5R0F6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein FAF1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0F6"/>
    <dbReference type="FunCoup" id="A0A1E5R0F6">
        <property type="interactions" value="238"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0F6"/>
    <dbReference type="InParanoid" id="A0A1E5R0F6"/>
    <dbReference type="OrthoDB" id="5556956at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053030">
        <property type="entry name" value="Ribosomal_biogenesis_FAF1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28096">
        <property type="entry name" value="PROTEIN FAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28096:SF1">
        <property type="entry name" value="PROTEIN FAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="342"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="382" mass="43000" checksum="18455F5C3D5B0619" modified="2017-01-18" version="1">MSSEDDHFKQQMELQRLAFEKQFGSLESMGFEDKTRVSSDSEQANTNSEGDSEEDGSDEGSVSASAEADDYGDDYGDDNDGDDDDDDDDGDKERDANENKLLQSQPKVTKSQPKIIKFDFSQNDVGAPVITKEERKLLKSGKSTIVKPNKPLQPGKSRKQKNVNDEEEKYNNNNDDDDDDDDNEADNLQKDIDLQNFIKDSHLLASFDPSNSSTSTHGTTVAHGDEMFGKVRMKALEQRFQTMKNAHNNVSAKKKQFQLESVPMNIRKGMLNKHMQRIAKHENFAKENGIVLSKVAKGKFRKIDFTYKKDIERRIGDSVKSKEKNFQKTMYRDKGLKINSVGRSTRNGLVISQKDIKRINGDGMSSGKKGGRGGKKSFGKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0Q0</accession>
    <name>A0A1E5R0Q0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Pre-mRNA-processing factor 31</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g4609</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0Q0">
        <property type="interactions" value="1229"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0Q0"/>
    <dbReference type="InParanoid" id="A0A1E5R0Q0"/>
    <dbReference type="OrthoDB" id="4771285at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027105">
        <property type="entry name" value="Prp31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019175">
        <property type="entry name" value="Prp31_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13904">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR PRP31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13904:SF0">
        <property type="entry name" value="U4_U6 SMALL NUCLEAR RIBONUCLEOPROTEIN PRP31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09785">
        <property type="entry name" value="Prp31_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Nop" evidence="5">
        <location>
            <begin position="250"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="471"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ80478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="495" mass="55521" checksum="F747F8CD83FBBB8E" modified="2017-01-18" version="1">MELETPEDADFLSDLENDFGELDDAKDYGHDQAHADGGPDVVIEQVEHMGVWNGKTFKEKIHGETQYAQESSNTQLSVLNEDGLCNISSNLDRFDLETSYVACSAFLLNSCGGLDSSAFNQMSFDELSKVLMTTDSCMRHVYQKLLISVPTDYSELLKLVRTKRLSVELLQVLLGAEKNNVNSVTLESQTKLSKEEILLVTLHLNNMALLDSQTRAQQEKELKPLITYYQNLEAIRFRFXSLLXSXVEQEAPNLVNLVGSEISTQLLQITSGLKNLVSVPNSNLPSMGKKYIQEHTEINQNXYTGDKAKGVLYYAELVQKQDTEEARKKALKMLSGKIVLCARVDFSKSDVSGGFGSRTKLELEKKLQNYNEPPLITHVKALPIPKDNAYIDGKDXKKKRRGGXKVTKYRNKFRLTKTQQLRNRMEFGKAETYLNDAYAEEDGLGLAGQKALLANTGSGGVVKPQLSKELEKRLRHSDKSETNDSIKMELDKDFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R110</accession>
    <name>A0A1E5R110_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ATP synthase subunit 9, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Lipid-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g4637</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. A homomeric c-ring of probably 10 subunits is part of the complex rotary element.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the ATPase C chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000016">
        <property type="protein sequence ID" value="OEJ80253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R110"/>
    <dbReference type="FunCoup" id="A0A1E5R110">
        <property type="interactions" value="831"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R110"/>
    <dbReference type="InParanoid" id="A0A1E5R110"/>
    <dbReference type="OrthoDB" id="438052at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033177">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, proton-transporting domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18182">
        <property type="entry name" value="ATP-synt_Fo_c_ATP5G3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.20.10:FF:000014">
        <property type="entry name" value="ATP synthase subunit 9, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.20.10">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01396">
        <property type="entry name" value="ATP_synth_c_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000454">
        <property type="entry name" value="ATP_synth_F0_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020537">
        <property type="entry name" value="ATP_synth_F0_csu_DDCD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038662">
        <property type="entry name" value="ATP_synth_F0_csu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10031">
        <property type="entry name" value="ATP SYNTHASE LIPID-BINDING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10031:SF0">
        <property type="entry name" value="ATPASE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00124">
        <property type="entry name" value="ATPASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00605">
        <property type="entry name" value="ATPASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0138">CF(0)</keyword>
    <keyword evidence="8 15" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 15" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="46"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="16">
        <location>
            <begin position="10"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ80253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="76" mass="7775" checksum="9858E92DA46906EC" modified="2017-01-18" version="1">MQLVLAAKYIGAGISTIGLLGAGIGIAIVFAALINGVSRNPSLKSTLFPFAILGFALSEATGLFCLMVAFILLYAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R179</accession>
    <name>A0A1E5R179_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">aminodeoxychorismate synthase</fullName>
            <ecNumber evidence="4">2.6.1.85</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Para-aminobenzoate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">p-aminobenzoic acid synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4273</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:11672"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58406"/>
            <dbReference type="EC" id="2.6.1.85"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 4-aminobenzoate from chorismate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the anthranilate synthase component I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.85" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R179">
        <property type="interactions" value="179"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R179"/>
    <dbReference type="InParanoid" id="A0A1E5R179"/>
    <dbReference type="OrthoDB" id="64220at2759"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00149"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046820">
        <property type="term" value="F:4-amino-4-deoxychorismate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008153">
        <property type="term" value="P:4-aminobenzoate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="entry name" value="Anthranilate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006805">
        <property type="entry name" value="Anth_synth_I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010117">
        <property type="entry name" value="PabB_fungal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01823">
        <property type="entry name" value="PabB-fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="AMINOBENZOATE/ANTHRANILATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236:SF18">
        <property type="entry name" value="AMINODEOXYCHORISMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04715">
        <property type="entry name" value="Anth_synt_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="ADC synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="10">
        <location>
            <begin position="9"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Anthranilate synthase component I N-terminal" evidence="12">
        <location>
            <begin position="334"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Chorismate-utilising enzyme C-terminal" evidence="11">
        <location>
            <begin position="537"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="domain" description="Chorismate-utilising enzyme C-terminal" evidence="11">
        <location>
            <begin position="771"/>
            <end position="839"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ80632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="849" mass="95946" checksum="F6C5EB657C512E76" modified="2017-01-18" version="1">MSRCIYLLFIDSYDSFTYNVVNLISNCIPGQDIRVTTIHNDSIDQLSVDLLQSFHGIIVGPGPGNPTDKVTIGILDQLFKIKTSSDSGDELGLKLRDIINAKPILGICLGWQAMCYYQDPQFARIEQLANIRHGQVYKMQLCDSFGQGTKKTLFENYPSQFESVRYHSLHVXLPFDYTSTGSLESVDGALLNSMKSIIPMVVCQDEDDGEILMGGKIRGKPWYGVQYHPESCCSELGTLLIKNFIQNVVLPESEHLQSSIDNPEYVAIRNLLNKKIDKTALLDTSCLSWPVSSCRENCKLVVKEYEPLIIKGDDHEIDIPGIVLRLGELIGPANFILLSSLMKQRNRGEYTVFGIPYDKKSNIYTYNCDAKCIATQKWNDFTKDAVIYKENMDRNDFWKFIELEMYKKKQTLQNQRVAHSKKFEESILNELPFIGGYLGILNYEMGDCVPLVNGGRSXDDAPDSVFVDIEQTIVWDHFGGKFYFIYNELHNDNGEFSALMNKIIRNLDGMGAVGDTSYKNNSKIDASLSYEIAKPLQKDYYQAFDKCQEYLHSGDSYELCLTTQCDVFVESTLEHHNNSDIGWEFFEKLTISNPAPFASFFSIPNCKTWFVSSSPERFLKWDSDLLDCELRPIKGTVKKTEDMTYEKAHEILKTRKEFGENLMIVDLIRNDLYELLPQVTMKELMSVEEYTTVYQLVSVISGSSLSNKDSAYTGLDILKHSLPPGSMTGAPKKSSVELLQTCIEPMVCAFDAXSTDTDSPNTHFSSINASHVRGLYSGVTGYYSLNGKGDFSVNIRCIFSQDNNKXESKNLKKHWKIGAGGAITVLSTKEGEWEEMLVKLNSTLQIFTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1E7</accession>
    <name>A0A1E5R1E7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative uridine kinase DAS2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4216</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1E7"/>
    <dbReference type="FunCoup" id="A0A1E5R1E7">
        <property type="interactions" value="235"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1E7"/>
    <dbReference type="InParanoid" id="A0A1E5R1E7"/>
    <dbReference type="OrthoDB" id="738517at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="2">
        <location>
            <begin position="51"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="262" mass="30191" checksum="BB2D398B9DEC438D" modified="2017-01-18" version="1">MGRYFISIGGGHCSDIHSITFSIREKLNQIFDNNYHIRIIDLDEMRGTKPFYTEQDFDFAKIYKDLITIKTSSNKSIGTEIQTQTPTQNTDVKEIILVCGVYALYNHKINSLMNLKIFMDTDLDFRLIKLITRNRDHENESQDKMNLESSIDLYMNHLRPEWFNYVKPTRFNADMIVPLKYNFDTMIANHDKTSMLFLDGIVSLINESDAAAANGETPQSALKLGNFNIAKRRTSSIYSSETPQLSFPEDFKQECPPAKSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1H3</accession>
    <name>A0A1E5R1H3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Glucan 1,3-beta-glucosidase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4083</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1H3">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1H3"/>
    <dbReference type="InParanoid" id="A0A1E5R1H3"/>
    <dbReference type="OrthoDB" id="62120at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004338">
        <property type="term" value="F:glucan exo-1,3-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050386">
        <property type="entry name" value="Glycosyl_hydrolase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297:SF9">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297">
        <property type="entry name" value="GLUCAN ENDO-1,6-BETA-GLUCOSIDASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184561" evidence="6">
        <location>
            <begin position="21"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="7">
        <location>
            <begin position="142"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="8">
        <location>
            <position position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="513" mass="59509" checksum="74946C858C2C57A8" modified="2017-01-18" version="1">MKYNLFILVFAAASFPGVISSLSYHHQTKAHNVYIPTHQKKXHXDXEPHETRILTPTHKKRENTDATXIDSQNANKKIYGVNLGGWLVTEPYITPSLYDHANSLLRSSEKTWEKEIVDELHFADYLGYNTAFSILRQHVDTWYTETDIQKIALLGLNLVRVPIGYWAWKDISNDMSQSTYLNGQISYSDTFINVGQLEKMRELLSWCQKYGVKAMIDLHGVPGSQNGFDNSGERNFYDSLRWLQELENDLANSNSTSALTVEILNSIFDEFITNNQYGDTVMGVQLVNEPFHWKVGSDKIIMFYSDMIRKYKRVQNMFDNTTPEAQIKLILHDAFEPLGFWNSFFTPAKKDILIDHHYYQCFSDWDLNKTDAQREKGVLXYGKELLSEQPHHPSFVGEFSAAIDDCATYLNGFGVGSRYDGTYYHTTKFESPLKLNETPLGTCVSHLSIDYWPEEYKRQTKNFIRSQLETYEKNSAGWIFWCWKTESAIXWDFERLVAFDIVPRNFKXLDHSX</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1Q6</accession>
    <name>A0A1E5R1Q6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutamine-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="8">6.3.5.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">NAD(+) synthase [glutamine-hydrolyzing]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3986</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>deamido-NAD(+) + L-glutamine + ATP + H2O = L-glutamate + AMP + diphosphate + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24384"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">In the C-terminal section; belongs to the NAD synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.1" evidence="8"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1Q6"/>
    <dbReference type="FunCoup" id="A0A1E5R1Q6">
        <property type="interactions" value="745"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1Q6"/>
    <dbReference type="InParanoid" id="A0A1E5R1Q6"/>
    <dbReference type="OrthoDB" id="2020662at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00334"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07570">
        <property type="entry name" value="GAT_Gln-NAD-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000036">
        <property type="entry name" value="Glutamine-dependent NAD(+) synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.110.10:FF:000003">
        <property type="entry name" value="Glutamine-dependent NAD(+) synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02090">
        <property type="entry name" value="NadE_glutamine_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014445">
        <property type="entry name" value="Gln-dep_NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090:SF9">
        <property type="entry name" value="GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006630">
        <property type="entry name" value="NADS_GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 8" id="KW-0520">NAD</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="9">
        <location>
            <begin position="5"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="716" mass="81332" checksum="5AD686F368294205" modified="2017-01-18" version="1">MSHLITLATCNLNQWALDFEGNRDRILESIRIAKSENARLRVGPELEITGYGCLDHFLEDDLYLHSWEMYASIISNPETHGILLDIGMPVLHKNVRYNCRMLSLDGEILFIRPKIWLANDGNYREMRFFTPWMKPSVVETFVLPPIIAKITHQRTVPFGDAVINTLDTCIGAETCEELFTPQSPHIAMSLDGVEIFTNSSGSHHELRKLNTRMELIMGATRRCGGVYLYANQRGCDGDRLYYDGCALIAINGKVVAQGSQFSLKDVEVVTATVDLEEVRSHRAAIMSRGLQSSLSTVKFERVTVEKELAVMSDRFDPKLAPTPERSIRYHLPEEEIAMGPACWLWDYLRRCNGTGYFIPLSGGIDSCATATIVFSMCRIVYKEIVDDGNDIVLQDCRRICRMGDSWIPENPEAICSKIFHTCYMGTSNSSKETRDRAKQLAERIGAYHVDLNMDTVVTSIVSLFEVATGKKPIFKIFGGSQIENLALQNIQARLRMLLSYLFAQLLPWVRSIKNSGGLLVLGSSNVDECLRGYLTKYDCSSADLNPIGAIAKKDLKKFIGYAAKEFDMPILIDFLDATPTAELEPITKDYVQSDEVDMGMTYDELGVFGYYRKVEKCGPYGMYLKLLHEWTPKLTPRQVAEKVKRFFFFYAVNRHKQTILTPSYHAENYSPDDNRFDLRPFLINPRFPWASKKIDKIVEQYENPDSTKELDVMTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1T2</accession>
    <name>A0A1E5R1T2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">H/ACA ribonucleoprotein complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3989</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for ribosome biogenesis. Part of a complex which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ("psi") residues may serve to stabilize the conformation of rRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the small nucleolar ribonucleoprotein particles containing H/ACA-type snoRNAs (H/ACA snoRNPs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
            <location evidence="1 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the GAR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1T2">
        <property type="interactions" value="636"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1T2"/>
    <dbReference type="InParanoid" id="A0A1E5R1T2"/>
    <dbReference type="OrthoDB" id="2187159at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034513">
        <property type="term" value="F:box H/ACA snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000454">
        <property type="term" value="P:snoRNA guided rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.230:FF:000001">
        <property type="entry name" value="H/ACA ribonucleoprotein complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.230">
        <property type="entry name" value="Probable tRNA pseudouridine synthase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038664">
        <property type="entry name" value="Gar1/Naf1_Cbf5-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007504">
        <property type="entry name" value="H/ACA_rnp_Gar1/Naf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23237:SF6">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23237">
        <property type="entry name" value="NUCLEOLAR PROTEIN FAMILY A MEMBER 1 SNORNP PROTEIN GAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04410">
        <property type="entry name" value="Gar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 8" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 8" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="130"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="152"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="237" mass="23720" checksum="2AA0060E158F7E9F" modified="2017-01-18" version="1">MSFRGGSRGGSRGGFGGRGGARGGSRGGRGGFGGRPMPQGPPDTVLEMGAFLHPCEGDIVCKSINTKVPYFNAPIYLENKTQVGKVDEILGPLNEVFFTVKCSEGVQAKXFKDGDKFYIAPDKLLPLDRFIPKPKVAGPPKPKNRKKKTGTAGPGGARGGFGGRGGFGGRGGSRGGFGGSRGGSRGGFGGGRGGSRGGFGGSRGGSRGGFGGARGGSRGGSRGGFGGSRGGSRGGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2D5</accession>
    <name>A0A1E5R2D5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Origin recognition complex subunit 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4046</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ORC3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2D5"/>
    <dbReference type="FunCoup" id="A0A1E5R2D5">
        <property type="interactions" value="844"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2D5"/>
    <dbReference type="InParanoid" id="A0A1E5R2D5"/>
    <dbReference type="OrthoDB" id="10265211at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20704">
        <property type="entry name" value="Orc3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020795">
        <property type="entry name" value="ORC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045667">
        <property type="entry name" value="ORC3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040855">
        <property type="entry name" value="ORC_WH_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12748">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12748:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07034">
        <property type="entry name" value="ORC3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18137">
        <property type="entry name" value="WHD_ORC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Origin recognition complex subunit 3 N-terminal" evidence="6">
        <location>
            <begin position="5"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="Origin recognition complex subunit 3 winged helix C-terminal" evidence="7">
        <location>
            <begin position="584"/>
            <end position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ81044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="718" mass="83268" checksum="7D081D2A03E088B8" modified="2017-01-18" version="1">MDIEDFTNSQKTLYVTKPKKSQRLILNEEFEDVGKQGKLKIPFISLVEGHEAIEATHKRWLLYNDLSLEFHKEVKDILQKSNRKIDEDLKRILQDADYTCNSPGTDRTCNMSDNNFKNVFIVGADNSFKLPSWDHNSNTADDLETELAKSAFSAYENSKKKVKMSPPLELIEYLEAEKVINNTFTVVIDMVSNECPNIRMMTKRSMFQLLNSITKYIEIEDDEDGSKHDGKEKYDLKTITALGLEYDLSIVQNFQKLFKKNLKIVFNYKEIELFNYQVLDDWINILNNLREQCKHFLSITNVFQVSTNLANIEKNLKIKTIRVLKSQGNHHVLKINDPLSLNTLINDIFKCFIKLSNGKLSFSTRFIAEIIIKLKNSTDSKKKLIHTLIKILDYSLMSYFYSSPFAVFLDIINFEQFYNDETYGHLLLKCPSFIHFVDSLVMGTGHSKFAANDTIEINDIKDMLNLEKGNKLNNFFVEMLVETNPLTQKLLELTDFLENDDNKIFSYNIFELYYHISITKSGSLENYLIENWPCLAPKLKLAKNKSISLSATFSDKSYIFHELFTLDNKKISGVISQNLFPTLRGNIEENLLSPERITYRSKCESYFKLGEIMDYPINYLFSLYREAGIVINVYDFYAAFKSILPKDDIVEFCKKDKSLKTIQTLINSDGSDTAFDKIALIWFMQCVTDMKYSGILKNELTAKRKFNGVVEKEIWRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2L0</accession>
    <name>A0A1E5R2L0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Glutamate 5-kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3939</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the glutamate 5-kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2L0">
        <property type="interactions" value="526"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2L0"/>
    <dbReference type="InParanoid" id="A0A1E5R2L0"/>
    <dbReference type="OrthoDB" id="409889at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004349">
        <property type="term" value="F:glutamate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901607">
        <property type="term" value="P:alpha-amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04242">
        <property type="entry name" value="AAK_G5K_ProB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21157">
        <property type="entry name" value="PUA_G5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.10:FF:000008">
        <property type="entry name" value="Glutamate 5-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000020">
        <property type="entry name" value="Glutamate 5-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00456">
        <property type="entry name" value="ProB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041739">
        <property type="entry name" value="G5K_ProB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001057">
        <property type="entry name" value="Glu/AcGlu_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011529">
        <property type="entry name" value="Glu_5kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005715">
        <property type="entry name" value="Glu_5kinase/COase_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019797">
        <property type="entry name" value="Glutamate_5-kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01027">
        <property type="entry name" value="proB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654">
        <property type="entry name" value="GLUTAMATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654:SF3">
        <property type="entry name" value="GLUTAMATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000729">
        <property type="entry name" value="GK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00474">
        <property type="entry name" value="GLU5KINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00902">
        <property type="entry name" value="GLUTAMATE_5_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PUA" evidence="10">
        <location>
            <begin position="304"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ81118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="411" mass="45148" checksum="DF0F850D5373FC2B" modified="2017-01-18" version="1">MXASKPLTIVIKLGSSSLVDEKTKEPKLSIMSKIVETVIKLRREFNHKIVIVSSGGIAVGLKTLNMKSRPKKMALVQAIAAVGQGRLIGRWDSLFQEFDQRIAQILLTRNDIADWSQYRNAQNTMSELLNMGCVPIVNENDTLSVNEIKFGDNDTLSAITASMIDADYLFLLTDVDCLYNDNPRTNPDAKPVVIVNEMSDVAQIDVSGGSGSDVGTGGMTTKLIAAELATSSGVHTIITKSEDPSNIYKIVKHIQKHAGSKDDSKYKEELGITVPLHTVFKAQDNQHQVKSRDFWILHGLVTNGAIIIDEGAYNALTRKNKAGLLPAGVIGVEGHFHESECVLLKLGYKDPKTGELDESKPLKVVGRARPNYTFQELNRIKGLQSKDIEEALGYADTEYVAHRDNLAFPPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2M1</accession>
    <name>A0A1E5R2M1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Adenylate cyclase</fullName>
            <ecNumber evidence="3">4.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ATP pyrophosphate-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Adenylyl cyclase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g3859</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adenylyl cyclase class-3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.1" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2M1">
        <property type="interactions" value="549"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2M1"/>
    <dbReference type="InParanoid" id="A0A1E5R2M1"/>
    <dbReference type="OrthoDB" id="2021138at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17214">
        <property type="entry name" value="RA_CYR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048580">
        <property type="entry name" value="CYAA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055071">
        <property type="entry name" value="RA_PHLPP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21187">
        <property type="entry name" value="CYAA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23010">
        <property type="entry name" value="RA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0115">cAMP biosynthesis</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ras-associating" evidence="15">
        <location>
            <begin position="1032"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="domain" description="PPM-type phosphatase" evidence="16">
        <location>
            <begin position="1724"/>
            <end position="1995"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="14">
        <location>
            <begin position="2042"/>
            <end position="2179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
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            <begin position="1"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
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            <begin position="534"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="634"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
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            <begin position="706"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="776"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="44"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
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    </feature>
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    <feature type="compositionally biased region" description="Polar residues" evidence="13">
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
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        <location>
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        <location>
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        <location>
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        </location>
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        <location>
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        </location>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="981"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="17">
        <location>
            <position position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ81128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="2398" mass="267382" checksum="E05D8AF8333C08A4" modified="2017-01-18" version="1">MNRDTLGNNQGDKNXFEEIKRTRSSGHLMQDTLKGMNDTHLLKTRSSSTSLKNLNSGPSSDTSANRFADHRQRSESLLSNSSSGGSSNKNSYSNLRTATSHPSKISQPEQVEDKQKRHPSGFLESVSKHGIHGHIHSGGNHGYVPTYNPNSGSKHGSTNPHINEKRQNDSTNATNVTNASAEPHKPHPYNAHASHHLRKKSPFFSLKRLTSTSSLPSFSDHHKDEDHGGSQSAKQKFKNASSALFLSPSNANSSVSVVNPSLDQQKHKKSSSKLKSLSPTNKGSSKTHTGSHNNSVLNDENANTTSSSVRSILNDNSHNPSKTSMNETSTTSFPMTPSTSNVSGKNFVDDLGSASTNVQIQQHRERLGSHYQQDHPHPERYERRGSTQRSAMPELPVPPPMKKTQSLFGLFNRPSNHHSSSSNYSSQGSQSSSQSSNHTSSHTSSHNSSHNSSHTSGLTSSGSSSSSLKSSKRAGSNHLLLKNPSQESHNDSNTINTITELKPKENSGNNEENIEDPRETVITAPVNVELPANVDGGLEKTHTNATDVPVKDSTEDEQAIRSTFDNIHTGEKKDDKNDKDVTSPFEQNTGDEAKADASNQPQPKKDSTAGIFSLDTNLDDLNDITSLISQQSLNLPAESSETTGKRKSIANNKGVRAAQGIDLAMPTTKNNMLGNPAKLPSASVQTSWKAPESWDIYFVDDSAQNSSNSLDSMSNTKKKTSKPLAKHSKEKMKKSDKKHYPKKAXGQDESGNLLATGQQASRHNYDRHPHKLHHENLENVSAKSKSLGNIKTKNPPGVTAENEGKLADTDGFSSASSLGSSMILPSSSDDDNDDIQLQAGDNPSQNSTFDQQRNDKLLPSASATDEQKGLEVNEXTLHGKSDLDKVDXGYPVALSRNPEKTTPRKTKQSQYHSSDFSSPGENDDEFHDEIILYKRKQSMQSQENKADLKAFRERTKKPDSSDSNATSSTQQSTPSDSTVSAVTDNSTTSSLPKGGMFNQRGLENSSTFIASEDEHLKYERYYNDLSDLNKKQTYVIRIYRQTEGXFTDGTFTCLSLYLDTTVQEIIPLLKKKFSLPQTSVFQLSLRIGKLSKILHHHSKPISIQIRLLLLSGYRKHHDALNLLGMDDLSFVFQFTFHPLISSQLSVEQESNLMRRGDFVRADLRDMNLTRPPIVFYQQAGDIESMDVSNNANMVLPLDFVESAISLSSIRMVNIRASQFPPNLCEVNKLVTLELSRNLIKRIPQQISKLENLSILNLQCNKLERLPQGFANLRHLQILDLSSNKFTAFPDIICECTSLLQIDLSNNRIQSIPQHINNLTKVAKINISNNRLRKVSDLSQMRNLRTLTIRYNFISVFKTNCESLQNIYVTGNKISVFDDTVPSLRTLEMHKNPLTSLFPTNDQLSNLTTLSLNKASLSSLPSVIWQKFVQLKKLELNENSLSQLPAAISNLTKLMYLSVNKNKLESIPETLPLLKNLKNLDLHSNNIRYLCSNFEKLQLTSLNLSSNTLDSLQEDILFDISDTEDRIPISKTLMFLSIADNALDNTAWSWLNLFKKLRHLNISYNKFSKLMYKDSFFLTELTELYISGNNISVLPGDIIFNNLRNLKVLMANGNRLQTLPAELSALRQLSVLDVGDNDLKYNISNFEHEWSWVENKELKYLNFSGNKRFEIFGTPVDGVDYSDLSALTQLKVLGLMDVTLKTFKVPDETFNLRSRTTESSVNGMNYGVADTLGTKDFVTSRDFTLEKFRGRANESLICLFEGKNEISNTNNRIPKVVRDIFADVFARALEKADDSERAIKNALRLAFLQLNKELNTMTIAAESKDMKESMEDPPITSIDLTSGSTATVVYIKGEQVFVANVGDTMAVLGKTFGEHAVLTENHSPNIMEEYTRIRIAGGYVNNNRVDGVSTVSRAVGFFHLLPHIHASPAIRNFTIARTDEIIIIATHNFWEYIDYETASDIAKMCQDRPMVAAAKLKDFAIAYGCTDSLTVICFTLKTEHANKNQRRITLNKKELRNIRSHNYEDNKLRALPVEIQPPTGNVAIVFTDIKNSTYLWELCPDAMRSAIKTHNSVMRRQLRVFGGYEVKTEGDAFMVAFSTPTRALLCCLTVQLKLLEVEWPAEIVSTKDGCLITDENNRVLFYGLSVRMGIHWGRPVTEMDIVTKRMDYLGPMVNKASRVSNVADGGQVALSADFVQEFHNILALQKHADQNSDLSKELYNDAEDLAYVGSEAKVLKEIGYHFYPLGEVKLKGLETMESITLAYPEALHKRYKMYEEESPGLFTSKESLFYLRQISLKLEYVLSCVNGGAYCISKGGRSTALSEFTKETQDSIFHNQSEDDLMDFLDHLITRVETVTCALSLRQKMEGALICDSQDGNIKKQSVFELLDSLFQLLEEKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2P9</accession>
    <name>A0A1E5R2P9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">peptide-methionine (S)-S-oxide reductase</fullName>
            <ecNumber evidence="2">1.8.4.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Peptide-methionine (S)-S-oxide reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Protein-methionine-S-oxide reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g4348</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(S)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:14217"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12315"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:44120"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[thioredoxin]-disulfide + L-methionine + H2O = L-methionine (S)-S-oxide + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:19993"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58772"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MsrA Met sulfoxide reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.11" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2P9"/>
    <dbReference type="FunCoup" id="A0A1E5R2P9">
        <property type="interactions" value="563"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2P9"/>
    <dbReference type="InParanoid" id="A0A1E5R2P9"/>
    <dbReference type="OrthoDB" id="77405at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008113">
        <property type="term" value="F:peptide-methionine (S)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1060.10:FF:000006">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1060.10">
        <property type="entry name" value="Peptide methionine sulphoxide reductase MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01401">
        <property type="entry name" value="MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002569">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036509">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050162">
        <property type="entry name" value="MsrA_MetSO_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00401">
        <property type="entry name" value="msrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42799">
        <property type="entry name" value="MITOCHONDRIAL PEPTIDE METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42799:SF2">
        <property type="entry name" value="MITOCHONDRIAL PEPTIDE METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01625">
        <property type="entry name" value="PMSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55068">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptide methionine sulphoxide reductase MsrA" evidence="8">
        <location>
            <begin position="19"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ81160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="184" mass="21111" checksum="29E1428C26532F70" modified="2017-01-18" version="1">MSTTVSRTIKYNSANNKLVTVAAGCFWGVDHLYRKHFGDRIVDCTVGFANGSEDKKDTDDSISYKTVCSGTTDFAEALQISYDPQAVSFKELIDFFFRMHDPTTTNSQGPDVGTQYRSGIFTHSDEDLKEALKLKEEWQPKWKNKIVTEVEPIKNYYDADEFHQKYLFKNPEGYACPAHYVRNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R398</accession>
    <name>A0A1E5R398_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histone acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g4041</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-lysyl-[histone] + acetyl-CoA = N(6)-acetyl-L-lysyl-[histone] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21992"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:21993"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R398">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R398"/>
    <dbReference type="InParanoid" id="A0A1E5R398"/>
    <dbReference type="OrthoDB" id="3361892at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032931">
        <property type="term" value="F:histone H3K56 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051236">
        <property type="entry name" value="HAT_RTT109-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013178">
        <property type="entry name" value="Histone_AcTrfase_Rtt109/CBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016849">
        <property type="entry name" value="Rtt109"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31571">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31571:SF2">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE RTT109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08214">
        <property type="entry name" value="HAT_KAT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01250">
        <property type="entry name" value="KAT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51728">
        <property type="entry name" value="RTT109_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ81043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="438" mass="49756" checksum="44D5F8F64FE2A9B4" modified="2017-01-18" version="1">MSLKQHLAKALPAGXTFEVYHFQSLPRAINGFVNRNSQDYQHAQVVKIQNFLSIAFNERIFFAIEIDTYIIMNTSTGALERHIFIAKADTNGYSDTKVKTGEVVSCFLKYLLSINCQSYLQKIVPLHRDYGQKGTAGMISKRTSTSKALTILSQRRCGHSAQAPTNFATFYQSFDIGKKIGHRKTESDGILTKISLFTRPERQYLFSESYLNPRKHLLTGSQLLRWWLDIVNKVSMETLELLNGRLQIPGENEXTIQKQYLASLNKSNNVWRVGSIYGDRRENVHSIPLFPDDPKTRFIKDLIGESRYMALNLDQFWKELQARQEFRLDVLVGVVGIVGLSKRADHWPQKEDVYVPLSLGKFKAMKSYVVGEEYNTVEGAVDARSNLHQYYCSKLGQSPLLVVGELDYAETRTKNSPAAVQKAAVNVLSDFAVRKKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R3L6</accession>
    <name>A0A1E5R3L6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphatase PSR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3926</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R3L6"/>
    <dbReference type="InParanoid" id="A0A1E5R3L6"/>
    <dbReference type="OrthoDB" id="277011at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000093">
        <property type="entry name" value="NLI interacting factor-like phosphatase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="361"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ81163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="540" mass="59022" checksum="D7ABB730AF5DE9E0" modified="2017-01-18" version="1">MGFISSIFCGSSDNHQNGTSASGSTHHGSTASSGPNSHHSKLRQKTSTPPTTTNINTATSANSQTNNGGRPTHKASPATSNGNLKKQRSSHSKNTMSTKTTTTRTKETVGANAHNGSSSSSSSSSRKQVKVANAHAPTNGYGNSQGTTTPTPRTSTSSSLSVTNEKSKMAKNENFEEEKNYTVVSEQVGEEEEEEEENEDNAENNKEKEPEDDVNYEMDVDSDSEQQPVSKTALHEQQQMTLQGGVPVDELQAALPHTINANSSVVVHETIQNSDSECSVPGTPPPASLTHNQQQHEASLPPPSLEXQQQYQDEQQQMMAQMSGEITPMMESNFIDLTKLQKGQAHAKGCETLLGIKDKEVFGNKKCLVLDLDETLVHSSFKYVRAADFVIPVEIDNQVHNVYVIKRPGVDKFLETVAKYYEVVVFTASVSRYGDPLLDILDNVKTNHGGKLNIHHRLFRDSCYTYEGNYVKNLSQIGRPLEDIIILDNSPASYIFHPQHAVPISSWFSDTHDNELIDILPILTDLSGVPDVSMVLDVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4M5</accession>
    <name>A0A1E5R4M5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome b-c1 complex subunit 2, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Complex III subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Core protein II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Ubiquinol-cytochrome-c reductase complex core protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g3497</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase M16 family. UQCRC2/QCR2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4M5">
        <property type="interactions" value="319"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4M5"/>
    <dbReference type="InParanoid" id="A0A1E5R4M5"/>
    <dbReference type="OrthoDB" id="6369905at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000021">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF209">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="14">
        <location>
            <begin position="16"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="15">
        <location>
            <begin position="164"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ81830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="360" mass="39385" checksum="FD365F81044A36C0" modified="2017-01-18" version="1">MLASRLQFARTYATKVSSKDAASKISSLSVKVHAGSRYATKDGIAHLLSRFNFEDTANKSALRLVRESELLGGSFKSSVDRESITLKAVFLKEDLPYFVNAIGNVLHKPSFKEYQLNESVFPVANVDTAARELCPLKKAEDVLYSITFRNGLGAPIYYDGVVPVSLEDLKKYAEKVYTKENIEVVGEGINEADLKRFVDDSLISALPTGQSLKNDVTPKFHLGETARERFTGANSVAAISVPVTPKDFATFEVLTQYLKSPLYEHFELVSDAKFDSYSGAGLFSLYVQGTNATKVSXNIKTIVSSLXSGKDISGAKSLTSTVLALRETPLSLDSVKNFKLEKFNYAAVGKVSSLPYADEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4T5</accession>
    <name>A0A1E5R4T5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">MIOREX complex component 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3773</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4T5">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R4T5"/>
    <dbReference type="OrthoDB" id="4070119at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014804">
        <property type="entry name" value="Pet20-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08692">
        <property type="entry name" value="Pet20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="291"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="296"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ81919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="557" mass="63387" checksum="AB3EE9C04F928CA2" modified="2017-01-18" version="1">MLASRFSLNRHGLAGACSRGRNAGLLDVRTTIKRLFSSGDDNKSSKPAINVYVSSGASLNSKDTDVKSLQEPAAKQSKLQKHKYLVPHVPNLDYISRKEIEIEKLFARDKPLFLGKFPLDLPSDTGSKVKSLGEFLETLQQTTTAEDAKNTQIKIINITENDGSSDSAEGATTTGNIDNILGKLQGSQNQLVKPWEASVTGIAFKEDAWKKVPKSVLESMKPYELKPLNRAEMERQKAANLKKKLGTGGRKRVSNSKRYQFGVYSSRINNGVGYFDLNDIPMFYQPPKISDPFDTLDSNQDAKSYGSANQKETSLTKTGNGNSRGQHVQQQYSYDPETNPXAVAKIRTERKKYIMHLAGEYNFLKEDQSTLRTEIMKLNKFLSKEFEKMRNISIQNEFRNYVLPLYIYVDHSSVSKRRFKSYLKKMIMSHIEPLLETVCAAFEEQKQGDSFRKKVNIKLSNAIKHLRNEIPSIYFTTDGIDCILQKSSVKGFGKIYWLKNSSRQLYIFNRKNADKSYIVGGDQTSYYRRSGLKNMKYPIKLISEKFDDAFKRWEDFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R502</accession>
    <name>A0A1E5R502_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">54S ribosomal protein L25, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3747</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R502"/>
    <dbReference type="FunCoup" id="A0A1E5R502">
        <property type="interactions" value="124"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R502"/>
    <dbReference type="InParanoid" id="A0A1E5R502"/>
    <dbReference type="OrthoDB" id="18529at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037507">
        <property type="entry name" value="Ribosomal_mL59"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040922">
        <property type="entry name" value="Ribosomal_mL59_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28041">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28041:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18126">
        <property type="entry name" value="Mitoc_mL59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein mL59" evidence="1">
        <location>
            <begin position="14"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="158" mass="18736" checksum="6313FE2663B645B5" modified="2017-01-18" version="1">MSSTNKKLFEYLPQQLKNFFHKYPPTVKYSSKPTSTLAPEANPFLPNKHPVTGRWHEPKYSLRRMSVLYKLSYRFGIQDLLPPTNSRKLFFEEKYNNKKFMKGVLSPKGHKYELERPAKLQRMADAIKNADKFILGNKGMKYKKRVEEKKDKSKISWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5W1</accession>
    <name>A0A1E5R5W1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3697</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R5W1"/>
    <dbReference type="OrthoDB" id="5572844at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018608">
        <property type="entry name" value="Gti1/Pac2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28027">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR MIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28027:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR MIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09729">
        <property type="entry name" value="Gti1_Pac2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="409"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="429"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="485" mass="54955" checksum="6E34DDA8F8273051" modified="2017-01-18" version="1">MTNQTPTMQNVVIETPLDVLILLQIVLLDQHTSSGNRTAQQQKFPLCLKRLDETCIKQLCQHGNIFVFKEKESKMKRWTDKLNWSPSRIVMNKFLVYKQLVNQDTLKKTNTNENDQNTININETNNKKGDKLMYTGLCKKTISLKFQNDIIHVVAYYNEKVQALQYISSEKRFEIHNNQHXLQTLTEIYGPTYPIHKELQENMEKCSVGTTASFSTNGSNSSTSSVSNGDNRSRSRSNSKSKKRTSSTSSSINNIKSNKYNGNNSEENLMDSLKMPLSPPHSIMESSSATHRPSIHPTEIPHFQLSPLHYPSSAYSMLASQNSALMTNASNTHRVQKQEMSLPKPXTIQNGLQILQQQLQKPYMSGDCTTNTHXHPTVTNIQYSYNPQNTLQYATTVPPFYQNLHSSATQLSLHSPPPPSSSSLPQQQYMNSQQDPSPQQHRPLFYSQGFFSQPVPVSKSHLLNSASYQQNQLPSVQDLKLPTIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R6D8</accession>
    <name>A0A1E5R6D8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphoribosylaminoimidazole-succinocarboxamide synthase</fullName>
            <ecNumber evidence="3">6.3.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">SAICAR synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3801</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAICAR synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.6" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R6D8">
        <property type="interactions" value="795"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R6D8"/>
    <dbReference type="InParanoid" id="A0A1E5R6D8"/>
    <dbReference type="OrthoDB" id="9991235at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00131"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01414">
        <property type="entry name" value="SAICAR_synt_Sc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000392">
        <property type="entry name" value="Phosphoribosylaminoimidazole-succinocarboxamide synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000015">
        <property type="entry name" value="Phosphoribosylaminoimidazole-succinocarboxamide synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00137">
        <property type="entry name" value="SAICAR_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028923">
        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001636">
        <property type="entry name" value="SAICAR_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018236">
        <property type="entry name" value="SAICAR_synthetase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010568">
        <property type="entry name" value="PRK13961.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00081">
        <property type="entry name" value="purC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43700">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE-SUCCINOCARBOXAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43700:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE-SUCCINOCARBOXAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01259">
        <property type="entry name" value="SAICAR_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01057">
        <property type="entry name" value="SAICAR_SYNTHETASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01058">
        <property type="entry name" value="SAICAR_SYNTHETASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAICAR synthetase/ADE2 N-terminal" evidence="10">
        <location>
            <begin position="17"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ82103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="306" mass="34721" checksum="FE6A7D1673F8C465" modified="2017-01-18" version="1">MSVPLTXTNLDNILPLIAKGKVRDIYEVNDSTLLFIATDRISAYDVVMENPIPDKGAILTKISEFWFDFFKNDIENHLMDIPQGKTLFDYLPSELYTNEKYRSQIENRSLLVVKHKLIPLEVIVRGFITGSAWKEYLSTGTVHGIKQAENLQESQEFPQAIFTPSTKAEQGDKDENITVEQAQQMIGKDLCGRLGNKAIELYCKAKEYAKTRGIIIADTKFEFGIKPNGDLVLVDEVLTPDSSRFWGADNFVLGKSQDSFDKQFLRNWLTSNKLNGVEGVKMPQDIVEKTRTKYLEALEKITGEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7F4</accession>
    <name>A0A1E5R7F4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Methionine aminopeptidase</fullName>
            <ecNumber evidence="11">3.4.11.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g3156</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 11">Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="11">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="9">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with zinc, cobalt, manganese or divalent iron ions. Has high activity with zinc; zinc cofactor is transferred into the active site region by the ZNG1 zinc chaperone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Associates with the 60S ribosomal subunit of the 80S translational complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="11"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7F4"/>
    <dbReference type="FunCoup" id="A0A1E5R7F4">
        <property type="interactions" value="874"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7F4"/>
    <dbReference type="InParanoid" id="A0A1E5R7F4"/>
    <dbReference type="OrthoDB" id="3209743at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004239">
        <property type="term" value="F:initiator methionyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01086">
        <property type="entry name" value="MetAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000010">
        <property type="entry name" value="Methionine aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01974">
        <property type="entry name" value="MetAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002467">
        <property type="entry name" value="Pept_M24A_MAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031615">
        <property type="entry name" value="Zfn-C6H2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00500">
        <property type="entry name" value="met_pdase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43330">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43330:SF7">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15801">
        <property type="entry name" value="zf-C6H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00680">
        <property type="entry name" value="MAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52013">
        <property type="entry name" value="ZF_C6H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9 11" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9 11" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C6H2-type" evidence="12">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>a protein</name>
            <dbReference type="ChEBI" id="CHEBI:16541"/>
        </ligand>
        <ligandPart>
            <name>N-terminal L-methionine residue</name>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>4</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>4</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>a protein</name>
            <dbReference type="ChEBI" id="CHEBI:16541"/>
        </ligand>
        <ligandPart>
            <name>N-terminal L-methionine residue</name>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>4</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>4</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ82835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="369" mass="41399" checksum="9E206F6C9FA80516" modified="2017-01-18" version="1">MVVCSGLQCGKPTESELKCPICLKQDIQSVFCNDKCYKENYKAHKALHYKEENDATPYDPYKSFKYPGTLKAQYPLTPKRHVPDSIPKPDWASNGLPVSEQINDRLNAIPVYTKDEIKKIRKACIMGREILDIAAAHIKPGVTTDEIDAIVHEETIKRNAYPSPLNYYNFPKSVCTSVNEIICHGVPDKYVLKEGDIVNIDVSLFHQGFHADLNETYYVGENIAKNAINTVETARECLKHAIKQCKPGTTFQELGDVIEKHAKENKCSVVRTYCGHGVGKYFHCAPTVPHYAKNRAPGVMKPGMIFTIEPMINEGTWKDISWPDDWTSSTADGKLSAQFEHTLMVTENGVEILTARNKKSPGGPLQRIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7H2</accession>
    <name>A0A1E5R7H2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">lipoyl(octanoyl) transferase</fullName>
            <ecNumber evidence="3">2.3.1.181</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3167</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LipB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.181" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7H2"/>
    <dbReference type="FunCoup" id="A0A1E5R7H2">
        <property type="interactions" value="409"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7H2"/>
    <dbReference type="InParanoid" id="A0A1E5R7H2"/>
    <dbReference type="OrthoDB" id="19908at2759"/>
    <dbReference type="UniPathway" id="UPA00538">
        <property type="reaction ID" value="UER00592"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033819">
        <property type="term" value="F:lipoyl(octanoyl) transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000544">
        <property type="entry name" value="Octanoyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020605">
        <property type="entry name" value="Octanoyltransferase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00214">
        <property type="entry name" value="lipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10993:SF7">
        <property type="entry name" value="LIPOYLTRANSFERASE 2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10993">
        <property type="entry name" value="OCTANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21948">
        <property type="entry name" value="LplA-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01313">
        <property type="entry name" value="LIPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="6">
        <location>
            <begin position="99"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ82828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="318" mass="35898" checksum="E80A6D6FE445E601" modified="2017-01-18" version="1">MLKRTLNKHCWKRFNSSTCTFTSKTRPVEESSRVLRHLHFVKPFMPFKNGLDIQENIVRVQLDMKKLESNIKRQLTALHEQNMAINDQEQSIIDKIMELKPNPVILTFQFEPTYTGGKRIKKQITKEQISEFEDFKPSGVTDPSKFVQLERGGQITFHGPGQMVAYIILDLKSFHQFPPKCYVNGIEEAALNLLDGYGVKAQRTNDTGVWSCLNNGKLASIGVNVNRNITSHGICINVSPNLEYLNHFTLCGLPDAKATSIQEILKDTKPNVGSNLVEGDETNTPSVDDVASSFANHMAKILGIEKVERMLIESHKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7M4</accession>
    <name>A0A1E5R7M4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative phosphatidylinositol 4-phosphate 5-kinase MSS4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3176</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7M4"/>
    <dbReference type="InParanoid" id="A0A1E5R7M4"/>
    <dbReference type="OrthoDB" id="20783at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF8">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 5-PHOSPHATE 4-KINASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="3">
        <location>
            <begin position="581"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="27"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="135"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="350"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="947"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="41"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="135"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="350"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="964"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="4">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ82888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="975" mass="110352" checksum="23EF50E5930432CE" modified="2017-01-18" version="1">MTIEMQRQKSDYSSNNIIITSNYKTKNIDTSDRNSTNNEGDNTKNDGDDTKNDGDNTNINAMNGNASMQRNKGGQQNGHSGENIYHSNSREKINHTNSGEKIYHPKGLLQSHSPTLHSPVPKSFEGKNPVINTSIFKNSGPNTPVSKLKGPVSNLKSPVPHTPVSNLKSPVPHTPVSNLKSPMPHTPVSNLKSPIPHSPPKLGRRSTSISNSARPPFHASKHSQIIALPQSQENDEGDESPKNDQYPHHLDTVKSADNDNMKTTTTLDHNNXNDQXHXXHHHHPEISRSNTXDKLKLQKKLEKVNTXLSKRANSIDQVSPMYSDAARSHSSLLITPNDYTNIDAATDKKIKSGDTLKEHKDAEPKEHKDVKSKETLEGEPKETVKGETHRLTTSSAPPALNGLAPLAVHKDYHNKEKIPTQDELHDQEQDLIMIDQKNNNKRYSITSTQTNNHINNFITPLSSLTTNESVAISHPGKKLSKTKSQLKTKFNWKPDHQQIADQKFKQTEKQHDQDQKTKERAKIQVQNKVDQIGKTISQGHTNFTIAYNMLTGIRVAITKLNSGTNTSNTITIKGNTLPTQGQENITTTTTTTTKTTKTTKTTAKIESNTKNRVPGQTLPASAFHETEKLKFISANSIQYSFKFKDYCPKVFSKLRRHFDINDDDYLKSLTSKYVLSELNSPGKSGSFFYYSRDYKYIIKTIHSSEHRHLRTHLKQYFDHCMANKETLVSQFYGLYRLKLPITYQLFNSSRTKTKSRKIYFIVMNNLFPIHIDRSFDLKGSLYGRITDCEPYEIQTSDGATVLTTSSATSTSSMTFKDLNWLQDNMTLNLSAPVYTYLKTQLEKDVGLLAKLNIMDYSLLIGIHDIRPDIYDYSNEEIFSWGILNAKKDQLYFIGIIDCLTNYSFFKNLETLIKSLNHNRNEISAIPPELYGKRFLKFIENGMVLDTTEEHEKXTENNKQTTQELSRTTKQRTLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7M8</accession>
    <name>A0A1E5R7M8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Something about silencing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3145</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7M8"/>
    <dbReference type="InParanoid" id="A0A1E5R7M8"/>
    <dbReference type="OrthoDB" id="1938992at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033255">
        <property type="term" value="C:SAS acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038988">
        <property type="entry name" value="Sas4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029184">
        <property type="entry name" value="Sas4_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38422">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38422:SF1">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15460">
        <property type="entry name" value="SAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Something about silencing protein 4" evidence="2">
        <location>
            <begin position="183"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="404" mass="46864" checksum="0D35DC55D14A8E7B" modified="2017-01-18" version="1">MTENSAPIQSNNTSRSVNVERNAVNVLVVKKKKKIDSFPKNYGRNVKSNNNKVDTNNESLSCLIPKSQSASFNEIQPRPIVHERVLRGSFPQKDSNKVPELQIHGGEDNTEEDSQDEKFDFDEHKIGITNESSLEFFRGKLPMSFAKISFPLTYMHKLKTLKSLNMEMKNTALETVTGISGIDPLNDALYENFHKHMCKEEKKMIQMDKIEAANLAERYETYLDTLNLVTWKXYLPKITKIENXQDNKEMERKKKLTIETITKLLSDYDSLKTLIKIRSFKLKNQDSLALPRGPLSKKIINPYQXLKSLPFVKCHTPSAQMRVNKNILTVYQRFDRMFILDYESSSDEEESNLKTSXAIKSYRRKRVFEKYKYPTIIIRCEAFDIVAQPFQNPKIVKKKNXSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7U3</accession>
    <name>A0A1E5R7U3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Pisatin demethylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3250</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7U3"/>
    <dbReference type="InParanoid" id="A0A1E5R7U3"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF166">
        <property type="entry name" value="CYTOCHROME P450 12A4, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="468"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ82985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="558" mass="64723" checksum="C8EA7A40D4E873D3" modified="2017-01-18" version="1">MLSGLFFFLIAVFVYSYIIYPIFLNKLNKQKIPGPLHLKLSSFFILNIFRKEQGIEYLENLHKRYGPIVQIAPNELSFNDIDSMKQIYLYGNFPKEYTTPKSKKIVTGFYSQFGNFNQRNLFSTGDTKLHLAKKKPLQKLYSKTFVMQQQSFIQGKINNLVKVLLNNKGTPIDVYALFCSLAMDVVSGFEYGFPNSSDFINELKYTNESLQIDETVFHSFRTSSSLWFYTTMFPSLYNFMVKFYQLDQKLDTGKQWIWNKLIKSVDLIDQEKENKTSETTSVLETLYKNYEKHLAKIGSEIADHVLAGHETTGITLSYITWQLSRPSNQHWQDKLFKEIDTQKDILGLGLXNTESQLNYNELDQKCPILHAIVQEACRLHAAIPGSERRYVPYGKPLKYQSFVVPQGTMVSCQPFSLHREARVFGKDVEDFKPERWLRMENELDSEYQSRIKEMEKHIMTFGQGNRMCLGMHLALIEIKACTASIYGNPLLTHSRISKEWCTNILANDKSAKIGYTGIGVKNKFDDPSVYKQLSDVDKMRMADSYTTRPLFDECWITF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7X4</accession>
    <name>A0A1E5R7X4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulatory protein UME1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3397</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7X4"/>
    <dbReference type="FunCoup" id="A0A1E5R7X4">
        <property type="interactions" value="735"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7X4"/>
    <dbReference type="InParanoid" id="A0A1E5R7X4"/>
    <dbReference type="OrthoDB" id="427795at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050459">
        <property type="entry name" value="WD_repeat_RBAP46/RBAP48/MSI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22850">
        <property type="entry name" value="WD40 REPEAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="287"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="398"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="405"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ83010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="462" mass="51611" checksum="146FE793AB4E17E3" modified="2017-01-18" version="1">MTNSEDQEQNAVLNEEYKIWKKAVPVLYSHITTYKPLLSEPLNASGENAVPKKGQELCTFTFENILAPVVEQANSGVCSSTVYYSRGKTLYSFDVNLPQGLLTQDTSLISPISVPSATALKLTNEYKVISTNDKHEYVKIKKLPQHQLLSLDTGNNLTWNHCVSKNVSMTYECENTGAPVTDFEIYKTEMIATSSGEIIDYKTAKQLSNLKIPQGVFASVHTQQQAPRFKNTNELVYINNDGTQLLSADTRASTASTSPVINYSKTADYTPNSKFTCFDHSHLAETLFVTGSTDGLIKVWDSRMATTPVHTLFHTQNENSTSAGVSKIQFKPNSPMEFLSVCADYGFIYQWNLEPLVNYQQSLAARPAEDADEMEITELDNEELNSRCLKYVHTSGSRRKRSKTVSKLENQTTNSSGSMGSAEKRQIMLNQCEWNPYMDDVVGCLDNDGTLVVYKGWFGQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7X8</accession>
    <name>A0A1E5R7X8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R7X8"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="243"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="347" mass="37815" checksum="63469F68E15BAF77" modified="2017-01-18" version="1">MRYNRRGKYSSSSYRGPSSINYEQEPQGRAANNEQQPTGSPATGASATFATGETYGQPAPGYGRGGSRFGGSRYAGVHKPSYYGGQQRYYDNNGRKTYAGSQYTQNAFTQESTQPSWSAAGHAEPEQQYTGSEYSADPVSRPASAKPAPSASASPVPPPSRSRFQNTTAQQHQHNAANTGSLSSPAHHPQHGGLSSWKRGPNRFGRPSTPLMERAQVSVESGASSYPYHSHASRXSTSSPRVAPTAAPAGSASNAAATSHEHFYAEYTKQLATAVVSEHDLKKASSLKNQIRDIYKDYDSINERARQNQFDRMFKTLEVKQSQVNYESLKLMNELNQECLDMILLQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7Z1</accession>
    <name>A0A1E5R7Z1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3386</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7Z1"/>
    <dbReference type="FunCoup" id="A0A1E5R7Z1">
        <property type="interactions" value="103"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R7Z1"/>
    <dbReference type="OrthoDB" id="4069757at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="192"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="237" mass="26347" checksum="0B5F4F9CCBFE1D69" modified="2017-01-18" version="1">MKTQILITDIPKCKFEQNWPQLLQTQLFNGTDAKFKYGSNEGDDVTVFTPLKSLHRIFIMCTNEKTALKLYHYLQDHPNINGTNNNTQSNQCNGKMHVYLTNSMLKSFSSQSSISTTSSSLHTATSTSTPPVLTVDTEGIEFMKKHKSCNTCSDGSLSPVTMDYSPGGHPVKMKRIDTGEYVYYNEPKPNKSRSNSSLLSINNSNSNLRGLNINSAKDSLNKTNQTSNPFIVINDAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R854</accession>
    <name>A0A1E5R854_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Damage response protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3448</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome b5 family. MAPR subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R854"/>
    <dbReference type="FunCoup" id="A0A1E5R854">
        <property type="interactions" value="836"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R854"/>
    <dbReference type="InParanoid" id="A0A1E5R854"/>
    <dbReference type="OrthoDB" id="547796at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000003">
        <property type="entry name" value="membrane-associated progesterone receptor component 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050577">
        <property type="entry name" value="MAPR/NEUFC/NENF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281:SF76">
        <property type="entry name" value="CALCUTTA CUP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="2">
        <location>
            <begin position="43"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ83068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="148" mass="16745" checksum="6CE7F5DE5B2EC89F" modified="2017-01-18" version="1">MSFLVNLLFGKVNVQQDPTGLVDSNSAANNLTEAEQPVVEQKFTPKMLNKYNGHDDPRIFIAVKGNVYDCTKGRQFYGPSGPYNNFAGHDASRGLARNSFEIENVKEWHQPIDDLKDLTDKERVALDDWEAHFKNKYALVGQLISENQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R8K5</accession>
    <name>A0A1E5R8K5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Vacuolar protein sorting-associated protein 30</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3367</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R8K5">
        <property type="interactions" value="539"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R8K5"/>
    <dbReference type="InParanoid" id="A0A1E5R8K5"/>
    <dbReference type="OrthoDB" id="20368at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043548">
        <property type="term" value="F:phosphatidylinositol 3-kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.40">
        <property type="entry name" value="Autophagy protein 6/Beclin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007243">
        <property type="entry name" value="Atg6/Beclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038274">
        <property type="entry name" value="Atg6/Beclin_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041691">
        <property type="entry name" value="Atg6/beclin_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040455">
        <property type="entry name" value="Atg6_BARA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12768">
        <property type="entry name" value="BECLIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12768:SF4">
        <property type="entry name" value="BECLIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04111">
        <property type="entry name" value="APG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17675">
        <property type="entry name" value="APG6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Atg6/beclin coiled-coil" evidence="5">
        <location>
            <begin position="172"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Atg6 BARA" evidence="4">
        <location>
            <begin position="340"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="113"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="260"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="136"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="6">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ82863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="530" mass="60623" checksum="0383C74F1D78DBBE" modified="2017-01-18" version="1">MPKDTPVLCCEKCKLPISLDESLMELSLVQRGSLTNAYYENPNISKGFDISLKDKPSSSRLNKLKNVKDLHQLNNSVLNSLESYVLLEDDREENEVRGAKSIFPERPASSNFNEKDSLYNNSTVGANSFPNTHGVGSKDSEEDERSRTLSRNLKALGNIFNILSSKSKIDHPLCSECCDLLLHELSKQYESGIKERDTYQDFLRKLKIQQQNNAMEKTANNNKTQTSDLESLSPVKKGVRRFSMEVQDXDLKIDDHIKELSTLDFEASKLMKTLETLEKESLKLDEELDQLEIEEHEIEAKNTELMETSNEMDLNTLSFLKDWGKLSAEYETLLTKYNDLRKINVYNEMFFISHRGLFGTINNCRIGGYDNNKVAWKEINAGMGHIVLLLSCIVNRLKIVLPSIRFEPMGSXSRVCTLEHHQDNSVTSPGDEVWKKYNCFYDRNTLTDEVKHFFTKENDFDKSLEVILLAISEIQKKIPNSDLPYVIENDKINGICIRLNGGQPSLQWTMACKFLLTNLKWLLAFSSRGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAA9</accession>
    <name>A0A1E5RAA9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GTPase-activating protein GYP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2552</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">Golgi stack</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAA9"/>
    <dbReference type="FunCoup" id="A0A1E5RAA9">
        <property type="interactions" value="1089"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAA9"/>
    <dbReference type="InParanoid" id="A0A1E5RAA9"/>
    <dbReference type="OrthoDB" id="26371at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000004">
        <property type="entry name" value="TBC1 domain family, member 22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF26">
        <property type="entry name" value="LD44506P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="4">
        <location>
            <begin position="233"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="57"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="502"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="68"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="514"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ83811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="596" mass="67959" checksum="3303B8B67B79028C" modified="2017-01-18" version="1">MSSKPSHTNPKEAGSRSLFSTLMRSMSASSVNMSSHHNNSNANRTHDIMLKTNNSAAAIPGKSDSHKSSTSNSSSRAASTNGINVSTSTFSTKNSSSKSLLHSHTSGKHSKKKKYEDYSEDWSATVDDYSVPVKDNDFLINEVRSSTHLSRTDSQGNLAKILSTTSSTTGNQLPKTKTLTEDEEINYSLERENEKELQKLNSKLSKISRFDDILKTSNNTINLSELRKVSWNGVPISHRPLVWKLLIGYLPANKRRHETLLERKRNEYQMGIEHMFSGKHQRDQSTWHQIEIDIPRTNPHIKLYQFKSVQDSLQKILYYWAIRHPASGYVQGINDLVTPFFQTFLTEYLPEKKLDMVEVLDPQDYLTTEQFVQVEADSFWCLTKFLDTIIDNYIHGQPAILKQVSNLESLCKRIDVDLYQHLQNENVQFLQFAFRWMNCLLMRELPMSIVIRMWDTYLAETSSDEDLEDETLSPTMESLNISALGGNTSSLFESSLRGSKGQFKKNDLTQKAPTSTNSAASSSMKRRTSLSEFHVFVCAAFLENWANVLLQMDFQGIITFLQNVPTTTWGEKEIEILLSEAFMWQTLYKDATSHWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAJ6</accession>
    <name>A0A1E5RAJ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2573</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAJ6"/>
    <dbReference type="InParanoid" id="A0A1E5RAJ6"/>
    <dbReference type="OrthoDB" id="4060534at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048920">
        <property type="entry name" value="REC102"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21736">
        <property type="entry name" value="REC102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ83917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="262" mass="30785" checksum="5DF4A2E461552DD1" modified="2017-01-18" version="1">MSHRLYEFETVYLKQKLNESTLISENDSQVILELVSNNYKNGSTNTQRITKMYDSIKQHQMVVIPSHRVEIVIESNSQIDISKLVEFFTNLQYRVLDISTSHSAEELYTYKFDVQCLLSMQNYMKPYQTFPMNDDDKQEVGDIDNISSDNRLHANLVHFSVNLKYFSTASQCKNLNKRKTSMFHDFINSLIISNLEYSYPLCFNKIVRKQYDNVMKYQQYYSKLNINYFDLVIILISSKESTSFIKLLRGGDSEFLPLPLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAL8</accession>
    <name>A0A1E5RAL8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NADPH-dependent alpha-keto amide reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2917</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAL8"/>
    <dbReference type="InParanoid" id="A0A1E5RAL8"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="5">
        <location>
            <begin position="55"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="4">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ83945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="327" mass="37315" checksum="1BFFF33B2DB6AA68" modified="2017-01-18" version="1">MTQTIQQKFFTLNKGSKIPAIGVGCGSKWLKREDTEDTFCEPLVEQLVYTLSLPGTVHIDGAECYKTYSEIAKAYAKIQSSVETKKHRSEIWFTDKYSLPTKYSDNPVEALDKQLSKTGMEYVDLYLLHAPFIDEEKYGFDIVGAWKYMETLFKQGKARNIGVSNFNVDDLEKILKXCEIKPVVNQIEFSPFLLNQSPGIYDFCIENGILIEAYSTLRLFLIDHDKKDPFYGYVDELSSKYXKSPXQVVLRWAEHKKVLPITTSSNPSRIAEAHYLYSDGFALTPEEVDQITLLGSKHEPLRQSWNSFEAYFTGKADAQTVAKSVTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAL9</accession>
    <name>A0A1E5RAL9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Pre-mRNA-splicing factor CWC24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2588</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the CWC24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAL9"/>
    <dbReference type="FunCoup" id="A0A1E5RAL9">
        <property type="interactions" value="312"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAL9"/>
    <dbReference type="InParanoid" id="A0A1E5RAL9"/>
    <dbReference type="OrthoDB" id="25761at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034247">
        <property type="term" value="P:snoRNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16539">
        <property type="entry name" value="RING-HC_RNF113A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039971">
        <property type="entry name" value="CWC24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12930:SF0">
        <property type="entry name" value="RING FINGER PROTEIN 113B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12930">
        <property type="entry name" value="ZINC FINGER PROTEIN 183"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="6 7 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 7 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="131"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="190"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="7">
        <location>
            <begin position="131"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="85"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="36"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ83960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="245" mass="28253" checksum="AFFEDBBBA1D6839B" modified="2017-01-18" version="1">MFKKRTVKKLQQKPSTTDLEKDSLSSNKETNIVPATDKKRSLSSKHTDETASLENDQQDSLKLLQPKKKLKLKQFEKVLINEETREEKEQTLSQRQQSETDLKEHFDETTDQDQKPANDNSTYHKVNVLVDYQPDVCKDYKLTGFCGYGDSCKFIHSRDDFIQGSKLSIDWKTGQPKSTQSQDKGIPTRCVICQKEYSKPVATSCEHYFCQKCFLQRLEKTPECFVCGKNTHGVMKPASKILKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAN7</accession>
    <name>A0A1E5RAN7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RAN7"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="164"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="579" mass="68457" checksum="F6548B0A8B85801D" modified="2017-01-18" version="1">MPVPPEIAKNIVSIWLNSLSQQELLEYQSAFGKYHYYHIIKPLIYKTLILGEYGNEFRNLLAWKLQLHNLKPIVIHDLDKFMHVLTTTTNGSLVKYFEKMDNYFERDSNDLSLIYQLILKNCKNLETIKYKFDKNDNCIYYLNNNVKLKNIFINCQYNLHHYTTNDSRSNSNTRNVESFQNNNNNNNNNNITNHNSISQESTNISNKTPGSRQVFFKDLSSIDVNLLRNAYDFPISRDLENLKYLKLYTTFKALPLEQKHEKAYFNNLLESLQEYNTKNVQRRERDFTLLFDEQEGRCPAINIFLKTLINENIYRFGNLKYLKFDHMSFTKDSIDLINKIFFKNCASHLKILEFNNVIEHRELMSREQIEALARDEPVAEGRYADYNNRIVDLPGVQDSLFNNAIPWKQFHALEKLVLNINPSEPTMLRILNQLQNVDCTAADFAFLKYLELSFFEHEAHHTDILAEIMQVLEYLKFGMTHDDHPPVNKLDGIEELVFNFNFDLSQLPIGLEKFLDDIFGYMRKTVTDFRLNIVGKFNQDIVSWRVGRSWRWKLLKNEKDHYNIVTAHXTYLDDNYVQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAN9</accession>
    <name>A0A1E5RAN9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="9">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2903</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Exact function not known, phosphatase 2A performs an essential cellular function.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the PPP phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="9"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAN9"/>
    <dbReference type="FunCoup" id="A0A1E5RAN9">
        <property type="interactions" value="1092"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAN9"/>
    <dbReference type="InParanoid" id="A0A1E5RAN9"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000786">
        <property type="term" value="P:positive regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903432">
        <property type="term" value="P:regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07415">
        <property type="entry name" value="MPP_PP2A_PP4_PP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000003">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="11">
        <location>
            <begin position="267"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="32"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="121"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ83980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="461" mass="50929" checksum="A3FCBCBD78E792BA" modified="2017-01-18" version="1">MDVDSDVPMQDANDANIHNNSTIEDATMIDVSNNNNNNTTTDSNNNNNNNNNNNNNNNNNNNNNSNNNNNNNSSSSSSGSGSSSNTSSSNNSNNIGSNNNNSETINGIDNDDSNAIHKVPSHSNNNSSSSAQLNSSSSAAINDSLKNSTAGLENGSASSVNQLDQWIEKIGKCQILSEQDVGRLCAMAIETLSKEQNVQPVSLPVTICGDVHGQFHDLMELFQIGGPCPDTNYLFMGDYVDRGYYSVETVSFLVCMKVRYPNRITILRGNHESRQITQVYGFYDECLRKYGSASVWKMFTDLFDYFPITALVDNKIFCLHGGLSPMIETIDQVRELNRLQEVPHEGPMCDLLWSDPDDRGGWGISPRGAGFTFGQDISEQFNHTNDLQLIARAHQLVMEGYAWSHQSVVTIFSAPNYCYRCGNQAAIMEVDENLNRQFLQYDPAIRPGEPAVSRKTPDYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAR4</accession>
    <name>A0A1E5RAR4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Aspartokinase</fullName>
            <ecNumber evidence="4 13">2.7.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g2752</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Phosphorylates aspartate, the first step in the biosynthesis of amino acids that derive from aspartate (the aspartate family of amino acids), including methioinine and threonine, the latter of which is a precursor to isoleucine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-aspartate + ATP = 4-phospho-L-aspartate + ADP</text>
            <dbReference type="Rhea" id="RHEA:23776"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:23777"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the aspartokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.4" evidence="4 13"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAR4"/>
    <dbReference type="FunCoup" id="A0A1E5RAR4">
        <property type="interactions" value="321"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAR4"/>
    <dbReference type="InParanoid" id="A0A1E5RAR4"/>
    <dbReference type="OrthoDB" id="4323675at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004072">
        <property type="term" value="F:aspartate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071266">
        <property type="term" value="P:'de novo' L-methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009090">
        <property type="term" value="P:homoserine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2130.10:FF:000001">
        <property type="entry name" value="Bifunctional aspartokinase/homoserine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000023">
        <property type="entry name" value="Probable aspartokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054352">
        <property type="entry name" value="ACT_Aspartokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001341">
        <property type="entry name" value="Asp_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018042">
        <property type="entry name" value="Aspartate_kinase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00657">
        <property type="entry name" value="asp_kinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21499">
        <property type="entry name" value="ASPARTATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21499:SF59">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22468">
        <property type="entry name" value="ACT_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00324">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 13 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0791">Threonine biosynthesis</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="14">
        <location>
            <begin position="449"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ83994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="535" mass="58521" checksum="E63507FBBD45C7CD" modified="2017-01-18" version="1">MASETDSNWVIQKFGGTSVGKFSEQIVNEIIKVYTTKYSNKVAVVCSARSSYTKAEGTTSRLLSAADLSANEDAELKDVVAILDLVRNDHVENAKTLIKNKALQEKLIDDTNKEVDLVEKYINASQTLGEVSSRTMDLIMSCGEKLSCLFIASLCNDNGLKAQYVDLSHIIPSDYSLGDNKSQLDSSFYTFLTMALKEKLQPIVDANSKGETVVPVITGYFGQVPYGLLNSVGRGYTDLCAALVAVALNADELQVWKEVDGIFTADPRKVPEARLLPSVTPDEAAELTYYGSEVIHPFTMEQVIRARIPIRIKNVQNPTGEGTIIYPDNLGRKGELTPPHPPTLMTQSFYESASSTKRSATAITSKNDIVVLNIHSNKKTLSHGFLAQIFSILDKFKLVVDLISTSEVHVSMALPIPDTINSKNLKRAVTELKILGTVDVLKNMSIISLVGKQMKQFIGIAGTMFTTLAESGINIEMISQGANEINISCVIEKEDTIKALQSIHSKLLSLNQGNTPSHEGTPINERLEQIKNLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAS2</accession>
    <name>A0A1E5RAS2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ADP-ribosylation factor GTPase-activating protein effector protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2750</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAS2">
        <property type="interactions" value="151"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAS2"/>
    <dbReference type="InParanoid" id="A0A1E5RAS2"/>
    <dbReference type="OrthoDB" id="10266696at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000009">
        <property type="entry name" value="stromal membrane-associated protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051718">
        <property type="entry name" value="ARF_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705">
        <property type="entry name" value="FI20236P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705:SF1">
        <property type="entry name" value="FI20236P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="7">
        <location>
            <begin position="7"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="136"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="183"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="278"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="140"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="183"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="193"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="217"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="301"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ83992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="330" mass="35641" checksum="ED5441883878DD72" modified="2017-01-18" version="1">MTEAKAKQVLNQLLKDPNNCYCADCKTSHNPRWASWSLGVFICIKCAGTHRSLGTHISKVKSVDLDTWGLEHVEKLVYFKNNGIANTYYEGKLTKNSPDQTKYIPNQSQLSSFIRTKYEMKKWVDSNVDVKALVKQNGGSSKATSASTNNSSASLSSKNSTTNGAPTNNNTLLDLNLNAMPSSVSLKSGSSSQAFARQPTVTSPTSVPSIQRAPNVVHSNGNIATKSXXNNXNKNDNRPELKKSILSLYAKPASASTSQLNSFQGNFKNTNMSNNSSMSNLFPSNTHSSASSVSSFSTSTNLPNPTATGSNTNNSNVSLEDNDLFKNVWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAS4</accession>
    <name>A0A1E5RAS4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Exocyst complex component SEC6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2863</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAS4"/>
    <dbReference type="FunCoup" id="A0A1E5RAS4">
        <property type="interactions" value="268"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAS4"/>
    <dbReference type="InParanoid" id="A0A1E5RAS4"/>
    <dbReference type="OrthoDB" id="190098at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051601">
        <property type="term" value="P:exocyst localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.70">
        <property type="entry name" value="Exocyst complex component Sec6, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010326">
        <property type="entry name" value="EXOC3/Sec6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042532">
        <property type="entry name" value="EXOC3/Sec6_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292:SF1">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06046">
        <property type="entry name" value="Sec6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="unsure residue" description="I or L" evidence="4">
        <location>
            <position position="822"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ83971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="874" mass="100855" checksum="C0B9716AB39B9F8B" modified="2017-01-18" version="1">MVYEEYALSKINNLLKDENALKNIKEIKEHLIKEKSTLDFRLKSVSDKNRASVNNGLQELDDSQKYVKRLKKKITEMTKLSQESTKSIERYDVIKKITKLQTIMEQTTGIYEKIVQFGDLVDNVTAMIDTEFENNQNDTLATGVPQLLMIHHQLTVARDFSEQCDVLGQISTDDVQTHLPRIFRNLESAITKFNQLFQLITNDLLELLTTKNYSLIIRFFKILEFEEKEDLKIQAIRTIVCLREKELEASKIRKISDSDSDIDVSLRNSTNNSHKRTSKIKDRLLKIKNTEEQQESANLQNKTTLSSGAVHLEEEEYNTPSDKALAEEILNGTITSRVYPRNYKKLFLDYLSEEIDLVFKRCWDHFEQEEQNTFAILNGLDWINGELFGIKRIFPLYCPAALPIFHIYFQVYYKNLNKIFTDLVNSEPETSMILNILDFDKEFFKFLNKELECSKEEIDGATSLLGDTAKQQLLDDYLNLILQKMQEWINNLEQSEFEIFKERKQPPQSDSENLLYLDSTKTCFQMFTQQVEVAADSGQVKILLGVVESFCKLLIDRQNKWILLINSEVNHWLDFTHQLENNPVLATMNKQEQAEYIAKNNSTDAPKLATPCSGGLIEYLIAVANDQMKAADYAVAISTKFGELVSKSNSRIINDHIEGTLDGFASVAKTNTNGLLRIIFDDLVIPYKEIFSKSWYTGNQAHQIRDTLNEYLTDISDQMNPYVFSTFIENVIEKTILQYLNALKYAHHFKTKNGKFVDKLQDDASIFFKMFCEHVSPEDLQTVVSDKFKFLEYFLELCVYPVSDIPTIWTSLLTKFHDCPMLLLGAILKCRKDVDSSASKQIMSQCLAIKTERSKQMAAEIDIGPTFLSDFNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB17</accession>
    <name>A0A1E5RB17_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein SGT1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2542</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB17">
        <property type="interactions" value="1024"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB17"/>
    <dbReference type="InParanoid" id="A0A1E5RB17"/>
    <dbReference type="OrthoDB" id="1898560at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06466">
        <property type="entry name" value="p23_CS_SGT1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007699">
        <property type="entry name" value="SGS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044563">
        <property type="entry name" value="Sgt1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45862">
        <property type="entry name" value="PROTEIN SGT1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05002">
        <property type="entry name" value="SGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51048">
        <property type="entry name" value="SGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="3">
        <location>
            <begin position="234"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="SGS" evidence="2">
        <location>
            <begin position="350"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="426"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ83763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="453" mass="51329" checksum="23EF313DD3BA9E4C" modified="2017-01-18" version="1">MLSKDINQIHKSIFVTEDPKKANKQCNELLLQKGGSQNAMLLAYKSIALLKLYFGSKVWRTDRTLTNIFRYLTKSLILVETQVSAEEDDKTKKKNETLGFIHFRFALLYYHLKQFGNSKSHLDIATKVYSYSSSAEPSLPTWEYTINEKLTTDKNEAISDNVKLDLTMFGEDDLQEDMQEINISEKVESFAEPESKINQTESPKELDEKKSHESVPTAIPESQSFLAAETKALTPKFKVDWYQTPQLVTVSLFTAHPPKSKDNVSCSVTHNQYLEVSYPNPDSESNSEFQYNVKLAFKVANEIEKVFVGTKKLEITLRKSKSGHTWKTLEFHENQTDPSVLQDTASSSSTYTIKSPYTSKVDWNKFGANISDDNVTAVPGDPNSVDIENESENEGGVDSFFQQLYKDADPDTKXAMMKSFLESNGTALSTDWQDVKEKKVETTPPDGMEAKKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB54</accession>
    <name>A0A1E5RB54_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2635</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RB54"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="353" mass="40724" checksum="8A6B6C1F6EE1012F" modified="2017-01-18" version="1">MIGLLCNLLNLTNFQXIPLYYTYQIYCCHVKYVNKDLEDPKXPTXSTNLVNKGENFGISSGXTVXKKSFLERRFSNPLFGYDTSASSAIEQVGITPNMILXKYFMCYVHLGVXNTEKSFWSGRENGEKXIVLYLNSVICNPSQTDPAVSGAXQITKFDKNFNINTQLHHVLKYWMMVVAYTALXKPVISFIGLGFFTNRYFESLVGVSFAISLFGNLFVKDYHSNNFLNDSFELLXHRLFTFYELLNWTVNYMQQRVFQNIDYKTWSNHKDGNSNPLVSSQPKREDKDAGRGMLYTYYTYVYDKGWEYLSSRLQVNALTQEQQAPQDEATASKEQRSSLELIQDYDIVDDFAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB60</accession>
    <name>A0A1E5RB60_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">DNA replication complex GINS protein PSF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3093</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the GINS complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the GINS1/PSF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB60">
        <property type="interactions" value="439"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB60"/>
    <dbReference type="InParanoid" id="A0A1E5RB60"/>
    <dbReference type="OrthoDB" id="10252587at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902983">
        <property type="term" value="P:DNA strand elongation involved in mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11710">
        <property type="entry name" value="GINS_A_psf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21696">
        <property type="entry name" value="GINS_B_Psf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005339">
        <property type="entry name" value="GINS_Psf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056783">
        <property type="entry name" value="PSF1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12914:SF2">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12914">
        <property type="entry name" value="PARTNER OF SLD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24997">
        <property type="entry name" value="PSF1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GINS subunit" evidence="7">
        <location>
            <begin position="40"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="DNA replication complex GINS protein PSF1 C-terminal" evidence="8">
        <location>
            <begin position="155"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ84137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="203" mass="23479" checksum="2DD734BB75A58F26" modified="2017-01-18" version="1">MYGDLATKLILESKRARKLRQSLNKNGKTIEAVVPEYQEEMVNKILKETEQLNSNAVFLQDSTQQNNKIKTCQYFVTLLSMERNKRCLLGYHKDRSDLIGEILWDKTNSTGTGFDINXLNHEEQQYMKEYSQLMSSLNGDFAEVDLTGSLKPPSEVFIDVRVLKDAGEIQTEYGVFHLIKDSQFFVRQSDVERLIQQGYLQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB99</accession>
    <name>A0A1E5RB99_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AWRI3579_g2944</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DEAD box helicase family. DDX6/DHH1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB99">
        <property type="interactions" value="1368"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB99"/>
    <dbReference type="InParanoid" id="A0A1E5RB99"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17940">
        <property type="entry name" value="DEADc_DDX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000114">
        <property type="entry name" value="ATP-dependent RNA helicase DDX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47960">
        <property type="entry name" value="DEAD-BOX ATP-DEPENDENT RNA HELICASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 15 20" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="5 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="19">
        <location>
            <begin position="41"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="72"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="316"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="111"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="488"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="14">
        <location>
            <begin position="41"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="111"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="137"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="521"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="559"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OEJ84172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="568" mass="64588" checksum="17682763B06F964A" modified="2017-01-18" version="1">MTENINHNAGQNSQQEWKASLNIPXKDTRPQTDDIKNRKGKNFEDFGLKRELLMGIFEAGFEKPSPIQEESIPAIINGRDVLARAKNGTGKTAAFIIPMLEKLKTSYNKNTTXTTTTXXNNNNNNQSNGSQTHNDNDNNNNNNNNNNXNNNYNNTNNTNNNNNNNNNNSNKGHIQSLILVPTRELALQTSQVVRTLGKHLNVKCMVSTGGTNLRDDILRLNEPVNVLVGTPGRVLDLASRKVADLSHCSMFIMDEADKMLSRDFKTLVEQILIFLPSIHQSLLFSATFPITVKSFMDKHLNKPYEINLMDELTLKGITQYYAFVEEKQKLHCLNTLFSKLQINQAIIFCNSTNRVELLAKKITDLGYSCYYSHARMSQQERNKVFHEFRQGTVRTLVCSDLLTRGIDIQAVNVVINFDFPKTAETYLHRIGRSGRFGHLGLAINLINWNDRFSLYKIEQELGTEIAAIPPNIDTSLYVAEDPTAIPMPFATQLPQAPPSMPDHHQQHQQHHNMQFPPPQQGNPQFHQQYPPHPQQQQQQQQQQQPNGQPHMPPQFAPQYPNNNQQFTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBA3</accession>
    <name>A0A1E5RBA3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2942</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBA3"/>
    <dbReference type="FunCoup" id="A0A1E5RBA3">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RBA3"/>
    <dbReference type="OrthoDB" id="4083608at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053092">
        <property type="entry name" value="Mitochondrial_unc_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28048">
        <property type="entry name" value="ACR195WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28048:SF1">
        <property type="entry name" value="ACR195WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="105" mass="11828" checksum="8F6DBFCB8B8383BB" modified="2017-01-18" version="1">MSNIVNLINPPPSRDLTPEETKDCVPCTIMSTAFALGFGTYLLMGKATEYDAKLATLKEHDSRNPKWWKTTLKVSGASLIIFGLWRGADGYMRYSEDKKRQKVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBV4</accession>
    <name>A0A1E5RBV4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">anthranilate synthase</fullName>
            <ecNumber evidence="3">4.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2981</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the anthranilate synthase component I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.27" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBV4"/>
    <dbReference type="FunCoup" id="A0A1E5RBV4">
        <property type="interactions" value="212"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBV4"/>
    <dbReference type="InParanoid" id="A0A1E5RBV4"/>
    <dbReference type="OrthoDB" id="1865897at2759"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="entry name" value="Anthranilate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006805">
        <property type="entry name" value="Anth_synth_I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005256">
        <property type="entry name" value="Anth_synth_I_PabB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00564">
        <property type="entry name" value="trpE_most"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="AMINOBENZOATE/ANTHRANILATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236:SF9">
        <property type="entry name" value="ANTHRANILATE SYNTHASE COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04715">
        <property type="entry name" value="Anth_synt_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00095">
        <property type="entry name" value="ANTSNTHASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="ADC synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Anthranilate synthase component I N-terminal" evidence="9">
        <location>
            <begin position="44"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Chorismate-utilising enzyme C-terminal" evidence="8">
        <location>
            <begin position="236"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ84366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="513" mass="57397" checksum="6EEECC273DE3B00C" modified="2017-01-18" version="1">MAIEVQPSVETIKECIEANNEKDASINMYPIYAFAPVTSEVNSPYTAYLKLSNLNDPNRAPSVLLESARNGNEIDRYSFIGVNPRKIIKTGENTEPGPCDPLVLLEKEMNQYKLAENIPGIPKLSGGAIGYISYDCVQYFEPTTKRPLKDVLKVPESALMLFDDIVAFDHVFQRFQVISNINTNEYKSVDEGYVAVSKRIHHIFNTIFRAGQTPQDYEPLQPPIKLDQTFTSNVGQEGYESHVTTLKEHIKKGDIIQAVPSQRVARPTSLHPFNLYKQLRSVNPSPYMFYIDYLDFQIIGASPELLCSSDTKNKVITHPIAGTIKRGKTLEEDEKLAAELSSSLKDRAEHVMLVDLARNDINRVCDPLTTSVDQLLTVQKFSHVQHLVSVVSGTLRKDKTRFDAFRSIFPAGTVSGAPKVKAMGLIGELEKEKRGIYAGAVGHWTYDGRSMDTCIALRTMVFKEGIAYLQAGGGIVYDSDEYDEYIETMNKMMANHNTVVQAEKYWAEKVGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RC84</accession>
    <name>A0A1E5RC84_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein BOI2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3041</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RC84">
        <property type="interactions" value="103"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RC84"/>
    <dbReference type="InParanoid" id="A0A1E5RC84"/>
    <dbReference type="OrthoDB" id="73680at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001881">
        <property type="term" value="P:receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13316">
        <property type="entry name" value="PH_Boi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09535">
        <property type="entry name" value="SAM_BOI-like_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11886">
        <property type="entry name" value="SH3_BOI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000230">
        <property type="entry name" value="Polarized growth protein (Boi2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000259">
        <property type="entry name" value="Protein BOI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035551">
        <property type="entry name" value="Boi1/2_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045188">
        <property type="entry name" value="Boi1/Boi2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22902:SF27">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY A MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22902">
        <property type="entry name" value="SESQUIPEDALIAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="5"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="7">
        <location>
            <begin position="305"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="6">
        <location>
            <begin position="907"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="79"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="172"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="438"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="620"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="756"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="835"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1053"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="116"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="172"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="193"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="438"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="456"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="477"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="515"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="529"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="585"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="630"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="697"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="726"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="774"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="845"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1053"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1095"/>
            <end position="1113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ84193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1184" mass="129209" checksum="56BFDD67D31B1D81" modified="2017-01-18" version="1">MQKMNRFPLYIVINEYNKRMDDELDMKPGDKIQVITDDQEYNDGWYLGRNLRTGFEGLYPRCFTQEISVNSKGQSLVRTKSSRRLISPGLNGSTTSLESDAQRHQPNMGFVPPQSPLGSXQRNFSTASSNSQHSHGNSPMNNGNNYPAVARENSSSLGIFNGATRRFSRLLSGNSTHSSTDVSPVAALNPANGKKDYSTNQTFKDDSTSATNSSANARNMMINSTLSDIDKALEELQNTPTPTDALYNNPNPAAAAAEAAAAEAAAAAEAAAAEAAAGGGENTAEKEALPHINPADLNPKDCGYWSPYEVAVYFESVGLDKPTCLKFISHKISGKILLDLQMIHLKQELDILSFGTRYEIFQEIENLKNVWQSSGSNGLQRNLSASITPSRSISHKKSSFLMPAASVANPVSSSSDQLPLPREKSQLTRPLSLALPLDSNNNEVLNSSSFVSPRKAPQPPSYPSPVQPPKSPEQSFVKRSSTTLVQKKTPSTTNFQNFGSLNGSFTHPYKMKIYGSSTNTPSPIQMQQSIKSSDTNSSSVSPTTSESTNENITNSKQNVQNSALXAGNKTRTDTDPVLKTHRSHSTISSSNPYGNSHKRSASGSSFIDLFNRLDMVNPQSIQQDGKERPTSSIYAHSRSSSFANSRKPSYTHSRNPSASRTTHNNPATGNSKNESRRGSMMSSFKALNDIGLNPQGNNNNNNNNNNNNSSSVLSTPASEKKGSFKRLQNSAKNSHNSDYDFFDSYADGYDEDDTENFLGTPAKSNKSFKRRSDATATESTAQYKTPMSNGTHLSTIVSGKSRSHISLASSSIPKNPRSTSDSVSTAQAAAAAAIKNSEKTERXLSSDSKIANNNQNTVSQTSMSKPELQHAFSSSNAINAKKKLKKKNTSAFQEGIKTITVQESMKDATCFGWMSKKGAGTVSGWKNRFFILHGTRLSYFQNTTATKERGLIDITAHKVLPARDDDKLVSLYAASTGKGRYCFKVVPPAPGSKKGLTFTQQRVHYFAVESKEEMRQWMAALIKATIDIDSSVPIISSCATPTISLTKAQEMLDQARRETQQREKERQEKLENGVLTQGDMSNDWEMQQRYITPEAGNSKNQSKTEKQGLSPSSGFASPYLLASGVFARNSVQSPGKENIDENIESNFAFSEVDTKETSYASPKIAQTSEYQYSTKLDPKYGNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RCB5</accession>
    <name>A0A1E5RCB5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein kinase C</fullName>
            <ecNumber evidence="2">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">AWRI3579_g3053</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RCB5">
        <property type="interactions" value="467"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RCB5"/>
    <dbReference type="InParanoid" id="A0A1E5RCB5"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20822">
        <property type="entry name" value="C1_ScPKC1-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20823">
        <property type="entry name" value="C1_ScPKC1-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11620">
        <property type="entry name" value="HR1_PKC-like_2_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05570">
        <property type="entry name" value="STKc_PKC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000101">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000103">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000034">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036274">
        <property type="entry name" value="HR1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037312">
        <property type="entry name" value="PKC-like_HR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02185">
        <property type="entry name" value="HR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00742">
        <property type="entry name" value="Hr1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46585">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51860">
        <property type="entry name" value="REM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15 17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10 23" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="REM-1" evidence="22">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="REM-1" evidence="22">
        <location>
            <begin position="107"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="20">
        <location>
            <begin position="424"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="20">
        <location>
            <begin position="491"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="872"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="21">
        <location>
            <begin position="1132"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="323"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="566"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="811"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="39"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="325"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="826"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="901"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="23">
        <location>
            <position position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="OEJ84233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1199" mass="136534" checksum="67144193D3FD2262" modified="2017-01-18" version="1">MNHSGQAQDQDIRNKIERERKIIQGATQLKQRTDNAMVIQKCNTNIREAQQNIEYLQDSLQRLSIQQHNELTTENTTALNGKPAMGNLRLSTISPQEQRFSRLDLAKYDCPSLAQRIQVMLQQLAFKLQVEKQYYAANEKMTKLYQIDGDQRTSSAAEFGVKESKNRIQLLNKAFKKYQAINVDADQTKGFSEDANLDMPADPKFTRKQLTGTLYFKIAAIRDVDHVASPIFSRKPETFITVKIDDTERXRTKPSRVDRWTDHYNIFIEKGNEIEITVYDKINEQITPVAITWILLSDIAEDIRKQKAVKQGQNGWVMAQDVMRSPTTNPSSTASGTSSNTLRPTTNMGQLHATDSARAVQSSESIEDPTKITTNAWFVLEPVGQILLTFGFTKQQSSKRKFIGGLNRHGAIIKRPDEVYEQHGHHFVQKSFYNIMCCAYCGDFLRYTGFQCQDCKFLCHKKCYQHVVTKCIAKSSADSVNEDAQLNHRIPHRFEPASNRGTRWCCHCGYILPWGRKNVRKCTECGVMCHAHCAHLVPDFCGMSMETANKILNTIKTTKVSSDKLSTVAPSGHASPGKLQYKPQPANKRSDAHIESLSALNNVRTPSVRRPPPEMLAPQSHLSQGVNNEIIDQVPSSLPNPPQNAYIQQYEXQQLQKVQAFVNRGSQYDMFSQNAIDEMPQDTSLNERDQLMEDYESNDAGEPVMPGTDLIADTQHNKXENHQSRLXTYRADPYHFGSSSTMFNVERTDEYDTSMVSEGQFAEVEANVTLPEPADDQKHHSYDYMEMQHLSQEIANXSPSNPFRKVVSAEQKXVSSEPAPDXWTTDSEHVTSKTKQDYEVRPSAEKIITQSTSPKKHKKKQVKRKKVSLDDFILLKVLGKGNFGKVLLARSKQNQRLCAIKVLKKDHIIKNHDIESARAEKKVFLLATKAKHPFLTNLFCSFQTENRIYFAMEFIGGGDLMWHVQNQRLSVRRAKFYAAEVLLALKYFHDNGVIYRDLKLENILLTPEGHIKIADYGLCKDEMWYGCKTSTFCGTPEFMAPEILKEQEYTKAVDWWAFGVLLYQMLLCQSPFSGDDEDEVFNAILTDEPLYPIDMAGDIVQIFQGLLTKDPEKRLGAGPNDALEVMREPFFRNINFDDILNQRVEPPFVPDIKGPEDTSYFEQEFTSAPPTLTPLPSVLNSKMQEQFRGFSFMPEELDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDJ3</accession>
    <name>A0A1E5RDJ3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1799</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDJ3"/>
    <dbReference type="FunCoup" id="A0A1E5RDJ3">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RDJ3"/>
    <dbReference type="OrthoDB" id="4040999at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052058">
        <property type="entry name" value="Alcohol_O-acetyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28037:SF2">
        <property type="entry name" value="ACR018CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28037">
        <property type="entry name" value="ALCOHOL O-ACETYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ84967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="516" mass="59917" checksum="33896185903A8232" modified="2017-01-18" version="1">MLARPLSEVEKTIALETLNNKRNGILSAVVFSSDQEEALREDDTATLASIRSYRVGSSLIKKALQLLVYENPELQVCINDDLKYEQIKTINYHDVLNKLDFDNVIDEKINCYHSIPLYLLREIFNKTKFELNKPLWRMFIVDENMLVFHGHESLFDNFSVMNLQKKLYNTIGTLQSKKNDQQQSGQVLFESKFAKHFDFPKSIFDSSKLYLPAVAKDLLQLQTQSFFKNVYLQTIKKPIDFFNSTPQYEILNHKTSIVFNFNDLCGSTVFGNISPSQFQNLKKALELENITLKTFMASLSMMCLQPFTASMNKDDFISFCFPIDLRKDLLYYEKDNASLGGLVYKKIIVECPLAMISDSAYDNATFHNGYDPKNVKIPKADPKFAEKLFEYKFNQVAAHIQSSVDQRVKVWKRNKFNDDDFKRMKFGKKDAQEQKYFEINDLTDFKLEPKGGKTSEKYKPKEIFFVKSENPESFMSLSFTYCELSGMNICIQYPEGYNMDDYVSNFETLITRLVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDN0</accession>
    <name>A0A1E5RDN0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ribosomal lysine N-methyltransferase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1895</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDN0">
        <property type="interactions" value="342"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDN0"/>
    <dbReference type="InParanoid" id="A0A1E5RDN0"/>
    <dbReference type="OrthoDB" id="42889at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19180">
        <property type="entry name" value="SET_SpSET10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044432">
        <property type="entry name" value="Set10/Efm1_SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF147">
        <property type="entry name" value="PROTEIN-LYSINE N-METHYLTRANSFERASE EFM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="unsure residue" description="D or N" evidence="1">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ85010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="551" mass="62192" checksum="3E26CCCA7D2FE73D" modified="2017-01-18" version="1">MNSSNEAKLQNFLIWGSAHGVHFSENCEFKYDHVKGISCVYQGNAPNSEPINVYAPXTXIVNGEYAQNCLEKALKKSLPSCNASVLLIWFLCYSRFGSEQXKRHFQKHKEYVDLLPAVVDTPLLWTDNAELEALTNTNIFASRQQKLDELARNWTSEKAIFGLAGTETTPTDNSHQVKPTDLAAYMWAYTIVTSRSFPEYVVNPDVTDKSRCVLVPFVDLLNHNPSARVEWSNTQTRARHNTGFNVKCDSIFSKGDEVLNNYGIKSNEELLFGYGFVIENNTADYXSLKLKLDENTYRALIKKGIQLNTASDYTTNAFELSENNLPTHTTNDEKIDKLVSEGAFFLLRKGDPHKVIKNLVSLFAYIEHGTADPTDLQAQLKGIRVLYENVQSKLKLLPTDEKLNELSKKAGNPYHVECARTYVGSARKILSYSMKYLEVLEQKKVKKHGAQCLTPKHLSMHPEVADFIRTSAHGAPLLEYDTELLLVIHSLVLGTDCXDQDISGVDEERIDEMSEILXSVKITISKKRLAQILSFYDERAQETLSGEILIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDN3</accession>
    <name>A0A1E5RDN3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Rho-GTPase-activating protein RGD2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1969</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDN3">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDN3"/>
    <dbReference type="InParanoid" id="A0A1E5RDN3"/>
    <dbReference type="OrthoDB" id="2155291at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005933">
        <property type="term" value="C:cellular bud"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050729">
        <property type="entry name" value="Rho-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF129">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN AT 16F, ISOFORM E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEP" evidence="3">
        <location>
            <begin position="255"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="4">
        <location>
            <begin position="619"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="367"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="376"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="393"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="426"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="458"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="470"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ84999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="842" mass="98053" checksum="FBCB554920C04260" modified="2017-01-18" version="1">MPYFTDSFWSPDFKEAIQTLFEGLFQNCQQNELFITFFANKMELEVMSAKKMISNSNDLVKKCELLVPNAKSSSLKDSSVMMMKNDNRNDVINANTGYETLIVLNQDLRVQGSQLLEIAELIETSVLQPFTKWCKEHKQRLEYSEKLLNDNITNFHKFKSNLNNKLEQQYINKCKLLQDFKTELPQHMIEEMDQILTQWKIWNKFLDKEKEYTHYTTLCNGSIDVDIRTMKMILRSLLLDLKKEDYKTSVFGYLITNTNNGYDIAKWLIQIMNLNNVKQAELIGQELINLNFLKDCNHNLSLKKSQFSSSKDLQYQWKTMAFDFIDIDQETLKSKINEHNVPDLXTTTSKLSINEHKVDTEGDVEINDXSKAQQKSKNEVLDKSESTQNIEATKSKETKENENADKEXNXENHKDFELDLDDDDFEKVSIEVETKDEKDQEAQKSKDSKTSKHEDTTTAESYTTQNASSEENEPAFVDEDLQPPPQQPRRSLTELEVRYEKILNDVKLANWKYLEGVEKADKLRCSLEELILDHLVFMEKCERDRLYALKRATDDLIHCYESKGQLMTLPTTNFKIEPELDLKNFVLKYRVGVYQPLPIIYRNYFGGGDPMMDKQVFGIDLTVRCRLDHSKVPKIISKFLQHFDDNVYPELPNDEVRCSIWTKSVKLNNSHKLRFKLNDSFKLAKFQLDGIEPSVIASVLKIYLLELPIPLIPQELNDILKKIYHMTDNNEDEDSAVKSNEKFHAIANVLSRIPKLHLATLDAISTHFVRLLSIIETSEENKDLSKKFIELISQEFANCIIRLTSFNDNVLGYKIFKDLLANKNQIFDLLKKNIKQINELNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDY5</accession>
    <name>A0A1E5RDY5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein MSP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2363</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDY5"/>
    <dbReference type="FunCoup" id="A0A1E5RDY5">
        <property type="interactions" value="591"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDY5"/>
    <dbReference type="InParanoid" id="A0A1E5RDY5"/>
    <dbReference type="OrthoDB" id="10254455at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140567">
        <property type="term" value="F:membrane protein dislocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140570">
        <property type="term" value="P:extraction of mislocalized protein from mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19520">
        <property type="entry name" value="RecA-like_ATAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000538">
        <property type="entry name" value="ATPase family AAA domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051701">
        <property type="entry name" value="Mito_OM_Translocase_MSP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644">
        <property type="entry name" value="AAA ATPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G12920)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644:SF3">
        <property type="entry name" value="FI08533P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="125"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ85094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="350" mass="39499" checksum="9DB232C5335063A9" modified="2017-01-18" version="1">MGRFAIDFKFLTDLSIIIGTGVSCYYLIARMSDIESPKSQTKESVEKQNENWQKLCRVNPALTDLTNQLNTYERQVLSNVITPFDINVGFEDIGGLDDTVEELVESVIYPLTSPEIFENNNLLTAPRGVLLYGPPGCGKTMIAKALAKESGANFINLRMSSIMDKWYGESNKIVDAIFSLANKIQPCIIFIDEIDSFLRERASTDHEVTASLKAEFMTLWDGLVSNGRVMVLGATNRIADIDSAFIRRLPKRFQVQLPNRESRTKICNIILKDVELDSGFDIEHLLDRTQGYSGSDLKELARNAALSAAREYFKQKRQLGSQYLSKKLRPLKMSDFFPDEAYAFSQQNLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDZ3</accession>
    <name>A0A1E5RDZ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="7">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1945</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PPP phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDZ3"/>
    <dbReference type="InParanoid" id="A0A1E5RDZ3"/>
    <dbReference type="OrthoDB" id="3972183at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000026">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF423">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE PPQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="9">
        <location>
            <begin position="726"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="60"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="179"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="255"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="581"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="8"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="126"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="197"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="263"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="320"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="424"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="445"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="475"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="490"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="502"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="528"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="581"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="595"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="921" mass="99290" checksum="AD449B115DB0761F" modified="2017-01-18" version="1">MGNSPSRNNEYINNVSANNNNSNNNGNAGSTGLDDGSNAESLDPDDRKYITSINDNIKSHSINDMKSTNTNDNMKSKSTNDNTNRNNHEHKSKSSNTSANPKTPRENLIGGYSSSTHSKSDNIDIKGTISKSGVSSTSSLSSSGKKKSKKTHALINDLYAGGTPYKQQSQMFKSAAGGGDFSAVKSTSSGKKHRRSSGTNINGYSSNNNNNNNNNSSTPTSPNLKTHDFEMDLEMAKAFSNYNFNLNNNXNMANFGNSSSGTNEDDDDDDDXXDDDDXXDDDDDDDDDGIFEGEGELGKRKKFXKNDAAGNPLERPSVASKSRCGSNGSTSSSSSEGSTSTVESALSTASTSSGSSTSSDNSDNTTCGSTTGARLASGSNLPHVSPLDSAKGGXRDGESSLLNSTRKSMDNEDDDDDGDEAGHDSPSSVVSGSIMSNANTTAVPSSSPSSASSNDSSNHSYNVLNAKNVANLPPIHEETSKDKGVVANLTTSKPNSSSTPTAMGTSIATNSSGSSEGQPMLLQRRKSSTSTSKYLNGLKNLSSSPTLIPVASHLSDMSASKHSSRSKSHAXXSALASASASASASSSSSTSSSLRHSHKHKSKKPTADYLNVDECIEKLLVMGDLREYKSKSFPFELWEIQLICSRAREIFLSEPSLLKLKAPIKVVGDVHGQYQDLLRVLKLSGYPGKTGYLFLGDYVDRGKQSLETILLLLLFKIKFPKSFFMLRGNHESANITKMYGFYDECKRRTRTSKTWKNFIDCFNTLPLAATINDKIFCVHGGLSPDLQDFHQINKIVRPTDIPENGLLTDLLWSDPDSNVSDWCSNNDRGVSYAFSKKNILEFCDNFNIELIIRGHMVVEDGYEFFARKKFVTIFSAPNYCGEFNNWGAVMSVTNNLTCSFELLKPKLHASNHSGSSKGKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE04</accession>
    <name>A0A1E5RE04_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphatidylglycerol phospholipase C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE04">
        <property type="interactions" value="112"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE04"/>
    <dbReference type="InParanoid" id="A0A1E5RE04"/>
    <dbReference type="OrthoDB" id="1058301at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46211:SF14">
        <property type="entry name" value="GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46211">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GP-PDE" evidence="1">
        <location>
            <begin position="5"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="386" mass="44891" checksum="27C556DE37700355" modified="2017-01-18" version="1">MMSTPWIMGHRGFKEDPEIKENTLPAFEKAMXTQSVDIIETDVQMTKDGVLVINHDQTTKRIYTXDCTINEVSYKQLKELVPKGMGKGVRMMTVKEFLKXIYTSETYTSNTNASEASIDKESIDKESTERGTSFLGENASKTSMKVIFDFKKNLDPIVFIKFYNLLLELGDELGDELGDELGDELGSHAGKWWXDKIYLGIWCLEHLEFGYIHSDMFQHFKIINISVSLQYSLKLSNWLVQQGLKPFDAVSMNFVAIWNDTPHQRQLKKDFPKETKLVVWTVNDLAIWEYTLKYINKLQWNVLAICTDKPVLFKKYYGNQHSVEVKKITQWKVVLYRAVYKTILFMLFSKWCQMNLVGFVNKMTWGNEVYVSVHSLFLGLLRMVFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE15</accession>
    <name>A0A1E5RE15_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2037</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RE15"/>
    <dbReference type="OrthoDB" id="4067935at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ85124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="305" mass="35872" checksum="CEBDFDA83B4F1607" modified="2017-01-18" version="1">MILDNIETKGLSSASAIEKDNLENEPCDCSQLDSDFETKNLCCTYSKQKTQLSQENKLILNQLIEQLSNKLKQSCLEYADLLLEDLMYPEGVEKSMLFLSTNNSESLHENYASLISNLGNTTTLEVLHIKKLLQKKVFENRSAELKQMEKIHVCFVVLLRNLSAYLEIPNAFHFFKELLKKNMTNFEKLFKLFXKYKQIITILMDCAFTHDPRSYDLDNXKQKNNSEKIEDHDIFQIKEQDLQLIQDFSMENLKWLEIMLHNSKAYKEYLQENSQMSKKIKYDDVEFKSFLKQRKSVLKISFRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE56</accession>
    <name>A0A1E5RE56_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcription factor PDR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1681</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE56">
        <property type="interactions" value="3585"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE56"/>
    <dbReference type="InParanoid" id="A0A1E5RE56"/>
    <dbReference type="OrthoDB" id="3364175at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF88">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="20"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="279"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="461"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1145"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1245"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1356"/>
            <end position="1411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="279"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="463"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1384"/>
            <end position="1398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1400"/>
            <end position="1411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1453" mass="165160" checksum="D7A6CADE265DFBFA" modified="2017-01-18" version="1">MEKDQPPKKKRQTNGKVKIACLNCRTRKIKCSNTIGPDGNLTTCTNCMIYGCPCEYVKGQVTVAKLDKAVQVSPSVSASASSQTTPTSAINNATTLQEADHLQHNKTSQPLTKTTSQQISNETEHKNNVLNDMQTQASSTSIXDFVNVKNISKDSTPTNQNYKNSTNGNIINITQTNSPIRVTDTSKSILASHNGKDAYEQTKSIKNFRFSDSVQPPLKTVETLHNSTINKPVSETDTSANVKEGHPISDQKNVMQSTDQTARGITNVKSIINPDIQESQEQQYKQEMDSLNNSPDKDSHTSPKKSQLTEEEIFAMNFRQNQILENTKENYDKSKENNNGELCKFLLKVDNQELRKQIDTLLQLISMDATLAEPLKVTINQLQYQLSMLDNAYSKDYVWNTKELNAIKHSNKVSSVESQILRNSKSKKIHLTNFNVFTHEEVTHISTKNAXTDNFEDIESAETASTSTSGTNSRSDTKITKDPFKDTPGGLGVYSLFSLFTKNGISVLLKHLNDYSGYEIKSENAEEPDRVCRIVSERELKETLYIFLKYAQYFLTNVNISYEMWSSPLDCFFINVMPMXDVKTDFEKCQELLKMVPIAVIDESFKFNPKFKSVYKNFAHFFSNATQAPLVFQWFEKLLDLHIKGIYHKTLVSVTEIKSVSEKIPEPIMADIWNDYHIEEAMNALVIYQFHRTLYGHMDSLEFLESLLSFFRRKMWCESSSILKSSLSFIIRIAQTLGLDRWEYYTGIDEELSERQRVAFWECSLWDVYISVQESTPTILNFSTLTSFYPKFMIDIGIVGKTSLYNFLAKEGYKFIDLDLNLKSKFLITCYTQIMADFNDKVLYNAKYTSINIMVQNGSNDLLFRELLDDIDYFQKYFNVLQLWFHNLNKEIKYKGFDNREQTDEYIWQIKFMGYACSYRTHMLYCCTEMVSRYSKAIGPEVLENSEYIDYTNLVNKYNMYVDECFKSSLSVFKLALRPDRFIALTHICVLLKRAMINIVCIHIFNHHQLTEEFLQLILTVLKNYYFLETWLFCKEFAKNRWVYKKPATLMQLKYDLTILCRILCQLYQEQEKMNKEDLLKFVSRKKFTSNNATAAYKLDDILAQVLDANTFEFDQLNKSRRSMNRKKYKKYLSEFAELSDTISSKVDHHSSPSAHVHTVRGNDGNELQNWKPAPLASSSAPPLFPYSGLPHPQVRRMPFQPTGVHPSVGNSSWQPPSSTVSNQRFNFAKHQLHGKVPISMLNNTAPASPNGSSGHPVNASPLNGQFPTQNQSPLPAIPHQAFSPNVQVMQSPRTADNQPYQIPAVPAKFPSVSDYYGEKPSLVPPPLSLNLQPHLYPPNLAPSTTLPGLMEISHGQNSTQMGSSHSHSSAVQNSPAASDKRPAMPGGEMMSMSSEASGINGTPSQTFSAGNNKVSIENINFGSLEEFINDANGWFHAWGDVGPEFNFSGFNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REH5</accession>
    <name>A0A1E5REH5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Iron-sulfur assembly protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2176</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HesB/IscA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REH5">
        <property type="interactions" value="294"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REH5"/>
    <dbReference type="InParanoid" id="A0A1E5REH5"/>
    <dbReference type="OrthoDB" id="3973108at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43011">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY 2 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43011:SF1">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY 2 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="138"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="167"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="243" mass="26466" checksum="22C4011A076F7D2A" modified="2017-01-18" version="1">MFSKVLFRNRLPFLSQSFTRSYVKNANESFVTPLKILTTNKSSDYVLTHRLNITERASNRLNEIYKESKEVLSILVESGGCHGFQYNLKLLPDAELNDLDLKECTVSKALTSATSNTTNNNXNTTPSEGLVAAEIKSKQEQAVASPETTAGASLTMESSTGSSLPEKEDEFGEEEFGEEEFGEDVDKLVIVLPTGAKLCIDSKSLQILNGTNLTYTTELIGSSFKIEGGNMKSSCGCGSSFDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REN1</accession>
    <name>A0A1E5REN1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2282</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REN1">
        <property type="interactions" value="1248"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REN1"/>
    <dbReference type="InParanoid" id="A0A1E5REN1"/>
    <dbReference type="OrthoDB" id="381190at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056802">
        <property type="entry name" value="ATR-like_M-HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058681">
        <property type="entry name" value="HEAT_MEC1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012993">
        <property type="entry name" value="UME"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25385">
        <property type="entry name" value="HEAT_MEC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25030">
        <property type="entry name" value="M-HEAT_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08064">
        <property type="entry name" value="UME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00802">
        <property type="entry name" value="UME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="UME" evidence="3">
        <location>
            <begin position="893"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1729"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1729" mass="199593" checksum="82B259A736109914" modified="2017-01-18" version="1" fragment="single">MTTVAEDIASTEKFIQKLRTKLSTNNEGISSFSLDSASSAYWFKNKDYSAIYHKEHQTNGMHRGVKALMLFVKKLANKCPNNTKLTQKVFEALNLLFKTEPLTFVYPVTTSGGARHKDKFKVQELDSVVYNLRSNKSCESIWYLFFSVVAIYKDSMKKKNSNFANLLLRVLTDDWLQIAFDYFGLYFEEVFTKELESCMDTMYMALMKDVVNNQPITPFEQDLDALPSNFDNTMPMDSDFEEGFELDLLIDYCFELSFYLSVAIXLRLSMDALLQKVIRLVFFILDELEQAYSLQSAVLLECTFLDLLSDYATTEEICITNSERATFTLQIYENTLTKMASNTXAGNXAESADKTEIECVLASALEKIVIHMVKHKSAGFVDISDSLNLEKWSQMYKFPDLPFGEKLNQQLFKIFSAYSTSTSSSIVDFASSLYEYFEKDLKDLKXLPAKVSACEFGSSCENSRNFFEFPMEDKINDVLKLSNLDSTQLISCLRLLGKLDCTLTQDSYCLHCDVSEHEWHLQKIANTRSELKHSVGFRCLQNYILNRDLFKLPNSVRVELLLCLKRIFNHFLIFQPTTQIESPLFKFLAQCCDSDLLKIRVTASQILVQYFTDGNDEVKMQVFKLLENKHPRHTIDTRLGTWTHLALSSRDDILDLLLLKLMNSVQSNDFLEECMAITEINYVASQINKQTPYQLLSPVLPVIVKNQAIRFASSPGIFKKLSKITHRPLDSFLIRFQGYIIPSSILIYYQKDILQQISNITKLSKHTLVENNMSKIVAFLLVKENVFSQKKLMTIISNCDSSYKNRSLQNCFDIYQTLSEICQLYDNPDIANGTSEAPFSNMLDILKSLYFVFDLVDNSFAKENKITEPDKKFIPVASVSSYEQWIQMHKTSFEVHFSRHILGIFHYFNKSMCDDVTMITYFDKMQIINGITFAIQYSAETSLASCLPQISMSLQVGMEILETRYQSFKAWYYLINALSLNQLLATIDIPICFIQQKWKFLDDKSKKLAYRIFGLLLKDNACLMRKPAVIYSLMKDVNINLVEKHKQYFNRATAXFVSXNTLEIFTNNLISYKNKYVVLQTLYNIEKFLKEGYKDIGKLCSKSVENLLGILLEVSYSFKNVNFQVSLKATECISYLGITNFTAYKIKQNTIDPSTFVYDLNVPIQQKAFMKRTINEVLVPSFWQTNDLLQQSYIAFAMQEYLIGCDFDSRSCNFEAEISRKEEWDLSFNELSRVTLEPLRSSKYNVSTVFTDYKSLTYPCYDKTLKFQQWINLFTSDIIKNIAKFNSNMYTSTQFDIFKVYCSLMKTSNIKFCEYMLPYAITHVLTNMSKEYTDLKKNIVHEILIIFKTEISLLNRHQVESLKMCYQTIFKVLEYLKKVVSQYGQKSPALSYLKKPQILEIEQLIQNIPLDLMAKRSLEIDSFERAVLYLEQSYRTNTLGSISMDXLRTYLQTAYSNIQDTDAIDGVLKTFTAIDLPSKLDELLYGENWDLAQKCLDELSNYDNNDEMNLKCLHSMNSHQLFEQSLIKMDKVQELSIIKSLKLEEYLTVGLESSVMTGXTKSLNKWNSLAERSSKSNVNANLLLNYNLNKALLTLSDSSCKKSKQFLENCFAIVTQSFVTSPDATTSLKIKDLLTKLHYLYNARVMSTSEETSXDAVLKFLNKRLAYVGDSFSSKFEIMCVRNSFAKHIKAYNDSFFNMYFDMSRLSKQAHRLDLTSKLLMDSWAQPLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REP5</accession>
    <name>A0A1E5REP5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative serine/threonine-protein kinase FMP48</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2070</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REP5"/>
    <dbReference type="STRING" id="56408.A0A1E5REP5"/>
    <dbReference type="InParanoid" id="A0A1E5REP5"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF91">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PLK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="3"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="40"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="391"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="545"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="590"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="45"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="548"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="599"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ85336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="793" mass="91361" checksum="60D85B2B3C3838B5" modified="2017-01-18" version="1">MIYKKISKIQSGSFSVVYKGMNQETGEMVALKCIPKPIIPTQPTNDDNSNNNNNSNSNSNSNSNSISISNSASGKDNKPIDPRMITRQQNKLKKIIKLVSNEYEILLKLQKHPFICCMLDFFETNEYFVFVLEYCENGDLYDYIKLVRNRTVNNPNNVEPPPKLDFFKLVQQIVNALQYSHSLNISHRDIKPENILLDSQLNVKLTDWGLSYMGEYNTNACIGTEKYLSPESFYPGYVLRDESIEMNSILPNYITHLLKDPQLLTVKGYNCMKSDFWSLGITLLYTLFASCPFKYACLNNPQQIGNNKKMTQQQQQQQQQQQQQGNRNFQVFITNPMKFIDEYYFQSIIRQETMNSMYYSNKTIHATMNIPDEKNYPAFWLTLCPQDTAATSTSETNGQTTQDFSTGKGNTKANETCGKKLYNSQFLETDKPFRIWMLHELAKNIVSTLLQLNFSKRNLGKFDEGLNLLWYSQTTVINNELNFHYDEHFKNYINNNNHTIDPLNNNSNNNHMENHNANNKSMEEVPITKEIMDLFYSSLELQHEQEQHQALQQQEMQQQEKHINRQESPLVNEPVQREIVQPFNNMHLQEQQQYHHHQQQQQQQQQQDHYQHPPSYSLHHMENIPMQIQHLYDLQHQNIQHGQHYSPHFQNHATPHEFLQQQQQQQQHVYADPVNMMYPSSTYSPAVPNGINANTNTNTNDINAHHSHQNYSNFSKLHNDHAVYGHNDINGNNANANANANGLGNGDNTITASITDVPSVHSTSTVISPSSAAKSPVLSVDGQTYISIAGTPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RET2</accession>
    <name>A0A1E5RET2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative oligopeptide transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2034</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RET2">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RET2"/>
    <dbReference type="InParanoid" id="A0A1E5RET2"/>
    <dbReference type="OrthoDB" id="627262at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045035">
        <property type="entry name" value="YSL-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645:SF0">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="145"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="307"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="352"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="404"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="538"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="570"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="715"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="757"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="795"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="458"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="13"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="477"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="824" mass="89391" checksum="8D8D4FAEB07ABAB1" modified="2017-01-18" version="1">MQTPTEQDMPLQDLTSTTPASSFNASTAGIFDASSSKNYSNTSSGRIPAXSQGQEGPTSNGNDPGLIDWNEKPYLKQVTLRGTVVGLFIGSLVLISNFQFGLQTGWVSMMSLGSALLACAIFKQVMPYIIPKSMSKTPFTDVENVYIQSIAVAVGTGPLCFGFVGVVPAIEKFLTVEEAGGIDFKGSISLFKLLVWSTSLAFFGVFFAVPLRKQVIIKEKLPFPSGSATATLIGVLNNSEIIHEVSRKELNRLREYSEVLETEMENASSPHDHYHHQHFSESEIPASGFEGKSTKKLSKVYYENIKILVKTFAVSSIYTIGSYFLPILKELPVFGNYLSKNYLWNFQPSPAYIGQGIIMGLPTTSYMMFGAILGWAILGPLCKKLRLVDPDADVHDWQNGIQGWVLWCSLTVMVVDSCIGFIVVTVKSLIKYYYENKRKPAVNIERVQVPLLAASNDTQEGAETQADDYGSLSEGSDALDDGLSEDESANFASPRALVASESQDNLNNNKPVKMNRSGSSVMYVSKDFDHDVKDPSLLVGQVTVISGIIISAFVCIFSMIYIYGMAIVPIYSVIWALVLALFLSILAVRALGETDLNPVSGIGKLSQLIFAVITPKERGGAVLLNLVAGAIAEAGAQQAGDLMQDLKTGHLLGASPKAQFIAQMIGASWSIVLSSVMYKVYNKVYTIPSANFRVPTAVIWIDCARLVMGHGLPKGAGTACLVLGIVFGLISFVKNIYRDHPNLSNRFSKFLLFLPSGVAVGVGIYNSPSFTIARFIGGLAAFIYVKSKHANRTKLIVFSSGLVLGEGICSIFNMIFSSLNVPHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REZ9</accession>
    <name>A0A1E5REZ9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator STP3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2092</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REZ9"/>
    <dbReference type="STRING" id="56408.A0A1E5REZ9"/>
    <dbReference type="InParanoid" id="A0A1E5REZ9"/>
    <dbReference type="OrthoDB" id="3973047at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="324"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="365"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="467" mass="53934" checksum="D8FDD0DD9C9C0447" modified="2017-01-18" version="1">MGYIHFDRVAVPNDNLGISLGQLHEQLQDIEKYEQKLLQNKENYEYNGKRNGDDDDDKYGMEDLIKTDNNDEINNENNIGEKNINNHQKNYFETDEKNFDDFLKDIMGYTSSANSDENIVSLQNNMINSSLQINETLDESFNKNDFYEESLDEEMNWNHYLSGASNTTTTTTDSDVNKTSSHNFINEIFDASVLSSPEMSFTMSPICADFLLGNRNTRDSVSSINSIGLFVLDEIHNDDNNDITNISVFDEIHSNIETSKIKKSRGRPRGSSNSTRKVTKPEQIEKIQQKNAKYAKKYVPKHVKEMHCVVINGITIKKTTDGKLPCPYCNTAIFKEKAYLIRHMKKHNPIKDFKCPFWNNQTKSHLCHHSGEFTRKDAFKAHLSSLHFLHEKKDATCTNDENDITKHTHNANTRGRCAECFQEFGSIKEWLIKHIETGQCKKMLIKDCAQGQKLRRRLEKKEHSLLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF57</accession>
    <name>A0A1E5RF57_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Putative transcriptional regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1991</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RF57">
        <property type="interactions" value="412"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF57"/>
    <dbReference type="InParanoid" id="A0A1E5RF57"/>
    <dbReference type="OrthoDB" id="2399539at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052202">
        <property type="entry name" value="Yeast_MetPath_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782:SF12">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="10">
        <location>
            <begin position="40"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="77"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="271"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="851"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="971"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1039"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1128"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1351"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1402"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="203"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1164"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="290"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="851"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="870"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="980"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1039"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1353"/>
            <end position="1367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1402"/>
            <end position="1419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1426" mass="164787" checksum="BD11AF56874B86EC" modified="2017-01-18" version="1">MGRPPKNVSKENIERFQLELKLANNDSKLLLGDKRGRSKSCLLCKRRKQKCDHMVPSCTACLKSGVKCIQPSNYGGVSKSNYNGNSNANDNDKNTVEMRNDNPVNENGDGKQADSNVYIPTSEPYSYNSKSAPKSKKIPGKDQQKDFANTYNAKNEQKNDYTKFLEKKLMYLENLVSISPASSAFEKKLQNYKKITQFLDVKLKDYEDIMNNIDSLRETEQQEQRLQLQHQKKQLLQQNSIFTENHEPSESNSQFSKPILPPPPLLNFIPMPSSMKPGVSRTAPSPNHTRTMDPARKMNGDSNAESYYGASNVTDRSEKGLLMANSLSTVKNIDDSLLMSRDCLGSIDFSKCVFSKYNLKDFLSYDPAFDFEEELSRQFLDTYFTRLQFKYCLLNQKEVYDFHEYYINLNNSSLNMNQSKPQNVYTPVQFHYCCSRMWLIFAVSACLHKTTGKYRGLPPNRYFSTAIRHITKCQDKLNNWQKVEILSLLLLYIIRTDRDSLELYDILKDVVHIVLHELHLNQYDDHDVLKNKRLRVFWCVYLLERMICNAVEKPYLIQEADISPNFPLFTYKSFRDAILPIDLSPSSSLSSPRKTSKSEELMTSNNILNERKVHFINQTLRLRRLESRFVTELDIMSFKTKNKSKKFLKSQLAKVEQYFHDLKIWRSSCNLQYVRNFENETLKLYYYRSVRLLIQPYLELLKPEHRLFRECQASAGQICQLYKIFHQKTVFGHSTPAVHTVFTSGLTLVYCLWLARNADDQRRRNLGDNAKHTRPTISATLFAVFDDLRACSVCLYVMAERSKFAIIFRDTFDQLMNCTIGNLIERCGPDSSELITLRNNDKKRSNLYSSMPLEESLSNEEPLSTEELNQRSLVQGNTGESHSTNSKSTLHLNKNDAFNTNNVKKGMPPAVSRTFGINQESEHAGFLQNLHIDLEEQQEMNRKKVALKKNMVPKSMTHLLAAYDEYENKEKRXKGLSVDTEEKRSKPETINKVEAQANIKETLPKQETSGAFTGANISYSHLAHSNANNLRKPLAQNSALRNVEPSSTSNLEGEQLENLSSSSRSPSSTKFVVQKPSLNIPDEEWKDFQTQALLQQQQGQQTLKAYLSSMFTNADFXNMDLSNTGWPLDGQNVQNTGNMYETQNGSPVHAQRLNTYDDLSQMRQEQYQLNFEQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQSGEYNLPKAAYRQQQAQHEYYYNTMKRNDSMLQPSTQNKKRKTAADLTVLPSASTIESAANENNVFLENIQANGVPLNTGVQNMINNISYWTSVNNEGSESFSMSEAPIVQENVPYYPRYESSANIDFQVNDLKQQHMQQQGQPYDQQSQFKQTDYPQPSYQHLVSEYDVQEYENVQHQSTTNYTDHLQQGNRKPDYWNTNNGYGGSNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFC2</accession>
    <name>A0A1E5RFC2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ATP-dependent RNA helicase DBP3</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">ATP-dependent RNA helicase dbp3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">AWRI3579_g1912</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent RNA helicase required for 60S ribosomal subunit synthesis. Involved in efficient pre-rRNA processing, predominantly at site A3, which is necessary for the normal formation of 25S and 5.8S rRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFC2"/>
    <dbReference type="FunCoup" id="A0A1E5RFC2">
        <property type="interactions" value="427"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFC2"/>
    <dbReference type="InParanoid" id="A0A1E5RFC2"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 16 21" id="KW-0347">Helicase</keyword>
    <keyword evidence="7 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="20">
        <location>
            <begin position="127"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="156"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="345"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="15">
        <location>
            <begin position="127"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="47"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OEJ85610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="537" mass="59958" checksum="93CD0BE393D9B82E" modified="2017-01-18" version="1">MSDIKEDKKRKNQHIEDVENGKVKKHKSDKKDKKEKKHKKDKKDKKEKKDKTSKKEKPEDLPAADAAPVEAKSSKKSKAPTQDIPAGTVYQQSAELTAAPQSEVDAFYAENEVEVEDPSKLGFRPLLSFSHINLDSKIQKEISKFPKPTPIQAVSWPYLLNGNDVIGVAETGSGKTFAFGVPAVNNLVTSGNKSNNVQVLVISPTRELASQTYDNLVILTEKLSINCCCLYGGVPKEQQRRDLKKSQIVVATPGRLLDLMEEGACDLSHVSYLVLDEADRMLEKGFEDDIKKIIGNTKSEGRQTLMFTATWPKEVRELASTFMNAPVKVSIGNRDELSANKRITQIVEVIDNFKKERRLMDLLKKYQSGEKKNDKVLIFALYKKEASRIERSLQYNGYKVAAIHGDLSQQQRTSALNDFKKGTSNLLLATDVAARGLDIPNVKTVINLTFPLTVEDYVHRIGRTGRAGQYGTAHTLFTENEKHLAGGLVNILNGANQPVPEELTRFGTHTKKKEHSAYGAFYKDVDITAKPKKITFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFC4</accession>
    <name>A0A1E5RFC4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Choline transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1911</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid/choline transporter (ACT) (TC 2.A.3.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFC4">
        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFC4"/>
    <dbReference type="InParanoid" id="A0A1E5RFC4"/>
    <dbReference type="OrthoDB" id="2417308at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015101">
        <property type="term" value="F:organic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000046">
        <property type="entry name" value="Amino-acid permease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004756">
        <property type="entry name" value="AA_permease"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00907">
        <property type="entry name" value="2A0304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF7">
        <property type="entry name" value="CHOLINE TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="74"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="121"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="271"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="326"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="380"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="406"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="439"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="474"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="550" mass="61256" checksum="C256C071A89D0206" modified="2017-01-18" version="1">MSEVIDSKKXEAVYDTSVESTSPETNEHYIEGEVTLRKSFSLWSILGVGFGLTNSWFGISASMVTGIVSGGPLMVVYGIIIIALLSIAIGGSLSELSSAYPHAGGQFYWSLKLAPPKYRRFAAYMCGCFAWAGAVFTSASTTLSVCTELVGMWALTHPGFVIKRWHVFVCFEILHGFLTIFNCFGKTLPYISASSLYISLSSFIIITITVLSCASGKYQDAKFVFATFYNETGWKNNGMAFILGLINPAWSFSCLDCATHMAFEVENPERVIPISIMGTVAIGFFTSFVYCISMFFSIQDLDAVLNSSTGFPILEIYYQALRNSKAGAIILGCLVLFTSFGCVIACHTWQSRLCWAFARDNGIPYSHWWKKIDPRLGVPLNAHLMSCVLIALLGLLYLASATAFNSLITGCISFLLFSYAIPIVLLLMKKRQIKHGPFWFGKFGWFLNIVTLGWTLFALVFFCFPFVMPASPNNMNYASVFIVGCTTYCIVWWKFKAHKNFRALEEDSDMLQEDAEYEMLQNNNNSSTSDSEVIVNAELVHHEQKTVKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFP0</accession>
    <name>A0A1E5RFP0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Nuclear polyadenylated RNA-binding protein NAB2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1760</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZC3H14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFP0"/>
    <dbReference type="FunCoup" id="A0A1E5RFP0">
        <property type="interactions" value="244"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFP0"/>
    <dbReference type="InParanoid" id="A0A1E5RFP0"/>
    <dbReference type="OrthoDB" id="438553at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043488">
        <property type="term" value="P:regulation of mRNA stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.40:FF:000003">
        <property type="entry name" value="Nuclear polyadenylated RNA-binding protein NAB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.40">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.40">
        <property type="entry name" value="Nuclear abundant poly(A) RNA-bind protein 2, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040366">
        <property type="entry name" value="Nab2/ZC3H14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043094">
        <property type="entry name" value="Nab2/ZC3H14_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021083">
        <property type="entry name" value="Nab2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049017">
        <property type="entry name" value="Nab2_Znf4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048410">
        <property type="entry name" value="Znf-CCCH_2-like_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14738">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14738:SF29">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11517">
        <property type="entry name" value="Nab2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21803">
        <property type="entry name" value="Nab2-zf4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14608">
        <property type="entry name" value="zf-CCCH_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21457">
        <property type="entry name" value="zf-CCCH_2-like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear abundant poly(A) RNA-binding protein Nab2 N-terminal" evidence="9">
        <location>
            <begin position="6"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="RNA-binding Nab2-type zinc finger" evidence="10">
        <location>
            <begin position="301"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Nab2 type CCCH zinc finger 4" evidence="11">
        <location>
            <begin position="382"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="88"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="145"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="207"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="512"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="236"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="246"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="512"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="528"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="553"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF11517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ85694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="574" mass="64696" checksum="6175250B27F4BFDC" modified="2017-01-18" version="1">MSADNAEEYSQCLNQFVSQYLSLIPNFNEDVNYVAEYIVLLMSNGGTVESVTTELQSLFESTKPEDIQHIVTKAFEAVQQLNNGATPEQVLDTVPKPGQQQQPVTQQPQQPQQQHQQQQHQQQQQQQQQQQQLQQQQQQQQQQQQAPVASQPVEAAAHVAQPVAEPTPQPQQNAQLPHFQFQFNNTETPAPSASPVTEDAEMQVFEPSSLSWKRDRDTPSARNSRFGGIQKGKPVNNTNNRNGANSRFQRNGPTTKFGQKNTQNKMLNAFNKIPLKTERCKDFPNCPLNSRECPGSHPTIICALYPNCERRNKTCSFLHPDEDTDLIEQLKKKKIEYNERKEEERQLFMEKKRMMTSGITLCKFGAVCANPKCPFGHPTPCNDDQKVSEYTWCPANLDCQDSNCTKAHSSPAKVKDIKPLGKPKAPTFASTPRYQPPIEKSLEQCKYGIHCTNKKCKYRHAKAPVMCRDGAACTRIDCVFGHPIPEPCRFGVNCKNKACLFQHPEGRVIPSTTWVNPDTTGTVNTPDQFQQQQQQQQQQPQLQIPQPTLEQQVQGQFTPGQPFNGQAADSAMQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGG2</accession>
    <name>A0A1E5RGG2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein mL50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1376</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL50 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGG2">
        <property type="interactions" value="326"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGG2"/>
    <dbReference type="InParanoid" id="A0A1E5RGG2"/>
    <dbReference type="OrthoDB" id="3980895at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018305">
        <property type="entry name" value="Ribosomal_m50"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10501">
        <property type="entry name" value="Ribosomal_L50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="285" mass="32367" checksum="7CF0C497C1261EAF" modified="2017-01-18" version="1">MFATRSGRKLICNSGMKFANQRTYNKTLNVTFVCADQSRQYSSLIDNAFGWFKSNNKAKKTTKATENTTSRELIEDIEKGALSTDTQNSAVASNSKENKENEEGSIKLNMREIEFIGKSGSKEXENTQRRKALLSKVKFNQWWNLEKVKDEKAFDNILHSINSELGLELNKPFADLASKFAFAKQLQQKTGYMLSDYDVTRFSTPALFKEHFSKKVFSGELARFNEHLPNAIYLDVTSEGGVINNAGNNVYLVPNVPSHEQKKTFNRINNEANYLRKQETSNASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGW1</accession>
    <name>A0A1E5RGW1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1133</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RGW1"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="234" mass="27523" checksum="D089610C438331ED" modified="2017-01-18" version="1">MSGSLNLKISELVEKRLKYYNVPVKDNDVKGSKQTRKYTEQKNEQVLALLKTLVTGNIDEAVEQVFEEYHDEVHQFYEPNVVNTVRKQKKDLLTSXSEQLVTKNVKENMSLLNRLDQNKNSYHQVQPELLDIDFLYAKHQFFTRYGKLIQEKLQREQDKKFEKXLEENQKDHENQRXHDNIDQSPSQSXLPHNGTNETDKLDEQVGLDEQNGHKPGNLQFKKDMXTYNLKFSPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGY2</accession>
    <name>A0A1E5RGY2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">U2 snRNP component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1044</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF3B1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGY2">
        <property type="interactions" value="1036"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGY2"/>
    <dbReference type="InParanoid" id="A0A1E5RGY2"/>
    <dbReference type="OrthoDB" id="3970592at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054573">
        <property type="entry name" value="PP2A/SF3B1-like_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038737">
        <property type="entry name" value="SF3b_su1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12097">
        <property type="entry name" value="SPLICING FACTOR 3B, SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22646">
        <property type="entry name" value="PPP2R1A-like_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Phosphatase PP2A regulatory subunit A/Splicing factor 3B subunit 1-like HEAT repeat" evidence="9">
        <location>
            <begin position="746"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="959" mass="107881" checksum="68857BF1AFAD906D" modified="2017-01-18" version="1">MSLPISFEDQKTRPFTDASRIELINSDPSFKNNDGESGAPPLSQLTKLDVAAQRFYNEQPVFKDQSDKRKRSMSVAGMTPISAVSKKSKTNEYSFPQISKSVEEDIKTTVITEFPGIKDLQYVRPTDAMYFAELLTPKKKSECTDEEQKQRTVLKLLLRIKNGTPQNRKHSIKELTDKSVKLGISMIMDKVLPILLDKALTEQERYLLVKLVDRLIFKMHSEIGPYLHKLFIVISPLLLDEDETTKNLGYEVIVSICKAVGPALIITTTRKDIDHESRDVREATARIIAICSKTLGPEHFIPFINAMCHSTKKWENRQTGVRAVHNIVLNMGLSAAPYLNYFLQCLDGLLEDSNIGVRMSTANAIDALATACNPYGASLYEAFVLDSLAKGIRKHNGKLLAAFLKALSNIIPIMNQEYTTYYGNYIMPIIQREMSSPDQVVLKAVLLSLQKCINAHGLLTVEKCKKEICPVFFKKFWTRRVALDLSLNKIVTYTTGLLAKKVGSQFMMSELIYHLKDSSEPARLMTVYAIEKIFKEADDIEAITGVVEXQLIDGLLIAFQDQKTDTVLFVRCFGTVANRLGTRMSKYLPPIISTVLNRLKNKTPLLRQLASDLCQXMVPECRKCGETEMLRKLSVXFYESLGEQYPEVLGSILGCLDSIVSNTANLEDLQPPITQILPTLTPILRNVNPLVNQNILKLVDQIARRSADSIPAKEWMRVCNELLDMLKSPIKQVRVQANETFGNIAKSIGPQEILLSLLNNLKVQERQLRLCSSIAIAIVARNCGTYTVLPALMNEYKTPDIHVQNGVLKAMAFLFEYIGPLSADYVYLLAPLLQDALTDRDLVHRQTAATILKNIALSCKGTGNEDFFIHLLNLIIPNIFETSPHAISRITNALDALRVALGPGIFMQYIWAGLFHPSRKVRKAYWTIYNSAYIQQADELVPFYPSFNDQQIAELDYVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH35</accession>
    <name>A0A1E5RH35_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Vacuolar transporter chaperone complex subunit 4</fullName>
            <ecNumber evidence="3">2.7.4.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Polyphosphate kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">SPX-dependent polyphosphate polymerase VTC subunit 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Vacuolar membrane polyphosphate polymerase catalytic subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g1127</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[phosphate](n) + ATP = [phosphate](n+1) + ADP</text>
            <dbReference type="Rhea" id="RHEA:19573"/>
            <dbReference type="Rhea" id="RHEA-COMP:9859"/>
            <dbReference type="Rhea" id="RHEA-COMP:14280"/>
            <dbReference type="ChEBI" id="CHEBI:16838"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19574"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Vacuole membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the VTC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.1" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RH35"/>
    <dbReference type="FunCoup" id="A0A1E5RH35">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH35"/>
    <dbReference type="InParanoid" id="A0A1E5RH35"/>
    <dbReference type="OrthoDB" id="6493944at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033254">
        <property type="term" value="C:vacuolar transporter chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008976">
        <property type="term" value="F:polyphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016237">
        <property type="term" value="P:microautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006799">
        <property type="term" value="P:polyphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07751">
        <property type="entry name" value="PolyPPase_VTC4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14480">
        <property type="entry name" value="SPX_VTC2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.100.30:FF:000001">
        <property type="entry name" value="Vacuolar transporter chaperone 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.100.30">
        <property type="entry name" value="VTC, catalytic tunnel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051572">
        <property type="entry name" value="VTC_Complex_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018966">
        <property type="entry name" value="VTC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042267">
        <property type="entry name" value="VTC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140:SF1">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE COMPLEX SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09359">
        <property type="entry name" value="VTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="643"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="669"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="709"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="17">
        <location>
            <begin position="2"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="490"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="507"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00075894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ86195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="731" mass="84252" checksum="A7D1DEC2AD904F35" modified="2017-01-18" version="1">MVKFGELLKRSLIKEYSYYYISYNDLKSELEEGLQKNDGQWSQNLETQFLEALELELDKVYMFSKVKHTEVLRRVKDAAQQVKVAVRSLDSNTPASELDFDILEQELSDIIADVHDLAKFSRLNYTGFQKILKKHDKVTKNFILKPIFQVRLDAKPFFKENYDDLVVKISELYDLVRTKGNPVSGDSAAGGKQQNFVRQTTKYWVHPDNITEVKLIILKHLPVLVFNTNKEFEQDDMAITSIYYDNKDMDLYYGRLRKDEGAEAHRIRWYGGMTSDTIFMERKTHREDWTGEQSVKARFALKERYVNDYLKGSYTVEQAFAKMRKEGKKSLKEIESLEALATEIQYTNLKKKLRPCMRSFYHRTAYQLPGDARVRCSLDTELTMVREDNFDGHDRTNGNWRRMDIGVDWPFKQLPEKDVERFPYAVLEVKLQTQLGQEPAEWIRDLVRSHLVEPVPKFSKFLHGCATLLPDNVDSIPYWIPQMGVDIKKDPVPSSINITRPDRGNDSDEDEDYDFDDEADDDNDDGQRFAAHGATGNLMDLEEEGYGSIPVSQDRRASTTQVIQGQSSNAPYINAKIRHATNPVTKMLYKAYGAFDHYMNGDQISKVAPGTVYDTQVKAPPGKTICVPVRVEPKVYFATERTFLSWISIALILSGVSTTMLTYGTQTTMVASIGFFATALLTIIYSTYVYCTRVVNIRLKRVVDYEDKLGPRLICLFLTLSILFTYFCNYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH98</accession>
    <name>A0A1E5RH98_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Exportin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1110</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the exportin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RH98"/>
    <dbReference type="FunCoup" id="A0A1E5RH98">
        <property type="interactions" value="1476"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH98"/>
    <dbReference type="InParanoid" id="A0A1E5RH98"/>
    <dbReference type="OrthoDB" id="27218at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051170">
        <property type="term" value="P:import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000490">
        <property type="entry name" value="CRM1p Major karyopherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041123">
        <property type="entry name" value="CRM1_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041235">
        <property type="entry name" value="Exp1_repeat_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045065">
        <property type="entry name" value="XPO1/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014877">
        <property type="entry name" value="XPO1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040485">
        <property type="entry name" value="XPO1_repeat_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223">
        <property type="entry name" value="EXPORTIN 1/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223:SF2">
        <property type="entry name" value="EXPORTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08767">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18777">
        <property type="entry name" value="CRM1_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18784">
        <property type="entry name" value="CRM1_repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18787">
        <property type="entry name" value="CRM1_repeat_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01102">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="7">
        <location>
            <begin position="34"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1066"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1066"/>
            <end position="1077"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1089" mass="123866" checksum="464005BE6E543569" modified="2017-01-18" version="1">MEAVLDFSKDLDISLLDQVVTTFYSGSGETQKQAQKVLTAFQDHPDSWQKADKILQLSSNSQTKFIALSILDKFVATKWKVIPANVRKDIRNFVAGMIIASCQNDEVFIKDKNLIKKSDLTLVQILKQDWPQDWPEFIPEIVTSSQASINVCENNMVILRLLSEEIFDYSAEQMTQAKTMKLKNSMAKEFEQIFQLCFQVLENGARPSLIVAALQSLLRYLHWIPYSYIFDTNLLELLTNKFLVAADTRNITLKCLTEVAALNLDGQINAQISMKTVQYFQTTIEKISSNVVPISANLKDMYINANSNDQSFLQDFAMFLTTYLAKHRVLLEEAGINNPILQTLNLSSHAYLIQLSNVNERELFKTCLDYWNIFTSDLFKEVQTISTKNLNPLMQLSLGTGAKPTFQGAQDPNYLKNFPLKKHMYDEVCSQLRLVIIENMARPEEVLIVENDEGEIVREFVKESDTIQLYKSEREVLVYLTHLDVVDTENIMVNKLSKQLDGSEWSWHNINTLCWAIGSISGTMNEDTEKTFLVTVIKDLLALTERKKGKDNKAVVASNIMYVVGQYPRFLKAHWKFLRTVVMKLFEFMHETHEGVQDMACDTFIKIVEKCKRHFVIQQSGENTPFIDSIIENIQSTTEDLEPQQVHSFYRACGIIIADENNRTNKERLLGALMTLPNMAWDAIVQGSMANPDLLLDSETIKIISNVIKTNVAVCSSMGADFYSQLGHIYYNMLQLYRAVSSMISAAVAKEGLIATKTPKVRGLRTVKKEVLKLVEIYISKAKNLDDVVNVLVQPLLNAVLEDYMNNVPDARDAEVLNCMTTVVAKVGNMIPDGVILILQSVFECTLDMINKDFTEYPEHRVEYYKLLKEINQKSFNAFLKLPPQAFDLFVNAIFWAFRHNNRDVEVNGLQIALDLIGNISNLQNSAFANEFYSKYYFSFISETFYVLTDSDHKSGFSKQATMLMRLIALVEQNKISVSLASGNSNLPEGSSNNHCLCTFMANMLKTAFPQLSEEQVFNFIGGLVKQYHNEAVFAGLLRDFLVQIKEYGGDATDYLFAEDKEQQLKEQRRVEREEASKIGGLLKPSELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHA7</accession>
    <name>A0A1E5RHA7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial pyruvate carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1077</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mediates the uptake of pyruvate into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial pyruvate carrier (MPC) (TC 2.A.105) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHA7">
        <property type="interactions" value="292"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHA7"/>
    <dbReference type="InParanoid" id="A0A1E5RHA7"/>
    <dbReference type="OrthoDB" id="869189at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006850">
        <property type="term" value="P:pyruvate import into mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005336">
        <property type="entry name" value="MPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14154">
        <property type="entry name" value="UPF0041 BRAIN PROTEIN 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03650">
        <property type="entry name" value="MPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="165" mass="18264" checksum="D2C59149BDF4E40D" modified="2017-01-18" version="1">MSFTRATINAGKKTQWSSKFNSFINSEAGPKTIHFWAPTLKWGLVFAGISDLQRPVDXISGAQNLSLLATAMIWTRWSFVIKPKNMLLASVNFFLGCTAGYQLGRIVDYRLKEGDSKSQVLDYIINGGKETGVKAPEQEANVNKPLIHHPLNDNNDVVIGDARTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHM5</accession>
    <name>A0A1E5RHM5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1393</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPL family. K-HECT subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHM5">
        <property type="interactions" value="1232"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHM5"/>
    <dbReference type="InParanoid" id="A0A1E5RHM5"/>
    <dbReference type="OrthoDB" id="423283at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045322">
        <property type="entry name" value="HECTD1/TRIP12-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HECTD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="8">
        <location>
            <begin position="1395"/>
            <end position="1710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="36"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="183"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="1677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1710" mass="194894" checksum="A8CEC432D981DC9D" modified="2017-01-18" version="1">MPHYNDDRESFDDEGDYSMNHSSTSEEDYPDRFHEEEEEEDDDDEVVDQDSSEYSSHIIQNESESEENAGMFYTVASDSEDETALGNHEEDEHDMRYSITEEPDNFGLVGEERGENPFGTLGERLRNVMSTSSDRDPRVEEGTTVHTFELPRAGGRPGRVEEDGFGEALLRMLSGAMNEGNRGHSNSTSXANGSRNDAHHETERINDVFAHLLRNVAQHDPRTRVHVGGSGMHSRQEYATIVLNIEKAEEDPYLALESLKKISTDILLMPPHILDTFVSLKNLSEKVINVMKNANLKEHAELQLQASQCLENVFNADEETIFFAVAKLDFLQVLKNSLQDMTFIDLAEQNLALLNIVSDLCSRSVLRIGIVQDVYNFIDFFPSHAQSKFLDTVSXCLTRPHANDIGYVSELSTLVTNMSFSSMHDKNCFVKCVKIYYNICANFPKKTYIKLIDLQVPVKMFEYFSTFKNDVESETSLKMLSILIALVNSNSQFLETVFDQKVKLSLFFNEFINIFKKREESTLVDILPSIPISHLRGLLKLLISVFHKEKAACEAQFDERGVRTITKXENEDEDFLISYNSDSAEICPNFGQNSFDFLDDCLPLVVQILSHTQDELASSYCLSLINISMSVLIXNGQALDMNDLGNLVSVLSSKAFALGKKFNENLDEHHFETEYSIRTLTQIFNKMLDMNTQXKEKILAMLKHEGLPKLLVSLYGSSKLSVTETTDKITRSAEEEASCVQSSKFENLTFESWMAIQKFLHPEKFETTEMCERQKAKYKKXNSRDSNYDYYGDADSFSYEDLEEYEAMLESGVECTYDANVDLSDPIYKVSETKKLLQMIKAELRNFKSAYEKLGVEYNDEEYQQKLQSFVKRLXECDVFSLDEQYWSLIWADLEECLHSRTGETLSFSELIDSNILSTMVQVVKRAPKECTTSLVNVLGQSSVTVLVGLLHNCIDRFEMFEQPYLFNLSNVKHDFERMSEPLRIEMKYCDEATDDEVPKTLKSFYLNVQPLIRIELLASTIKDLWVKNIMKDTSLSTERREAFMKESTKWNFTLCDSSLRPLNPHLTALGQLIKDLNSTKNASDLKFNWSSIDFGISWGFQKTYLPQGTQPADVPGKNSTKHSTFGTNYANKFDAKIELNPNIANSLELMSAISAVNSYNNCDSACFINNKLTTKLHLQLSQPLITLTGLISDWVFYMCSHYPIIFTYEAREKLMTDTCRGTKRLIKSWVNELFGNQDNNNANNEHNIENFAHVPGSLLWRVSAINTHKIVIDRKDMFDQALKYSSSISDPANVLSVKYTNEKGEGSGPTREFYSILSKMFTRKSLNMWRGSLDSNPQDHNVKHLKFVGKAVDLDLDLDLNLKADTAEEHTATDQNKNEDTNDQSENHFCLQELFPQPLVFTERENNAVASLFKFLGTFLARSLLDLEMVDFRFNEVFFQLLDTYVENGCENKNLLKLMETLSIPDGIDLITKIDKGIGKSFRFIYEQGLKNTSDNTGTCKDLLELNIPYNLPGYDSFAMPSSSASTEEYVSNANYKEYLRTCINMMLCEGILPCLQNFYEGFNTVLPYEALLFLTPKETSLYFGKIDEDWSFETISKSLNADHGYTMESNEVVNLINIMTSLSKNERRLFLQFATSSPRLPLGGFQKLRPKLLIALKPPADNCSPDDTLPSVMTCVNYFKLPQYSSIDVMRKRILQAITEGSGEFSFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHS3</accession>
    <name>A0A1E5RHS3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Karyogamy protein KAR4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1653</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MT-A70-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHS3"/>
    <dbReference type="FunCoup" id="A0A1E5RHS3">
        <property type="interactions" value="1082"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHS3"/>
    <dbReference type="InParanoid" id="A0A1E5RHS3"/>
    <dbReference type="OrthoDB" id="14833at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036396">
        <property type="term" value="C:RNA N6-methyladenosine methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045123">
        <property type="entry name" value="METTL14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007757">
        <property type="entry name" value="MT-A70-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13107">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13107:SF0">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05063">
        <property type="entry name" value="MT-A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51143">
        <property type="entry name" value="MT_A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51592">
        <property type="entry name" value="SAM_MTA70L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ86458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="303" mass="34918" checksum="A8A0C61C85F6DA4A" modified="2017-01-18" version="1">MIQTPTFTQSSPLMNDYTDHYVHAGSFPQSHVENIEDPVIGYPKLQKLLELKKRQVVDYATKPYGCRIDVDRMGPTLNKWVHLDMLSFDVVMIGCLSDNQFIYPLLRSIPIDKLVSKPGFLFIWASSKKINELSRFLNSEVVAKKFRRSEELVFVPIDKNSNFYPGLNEDDDALFEKMQWHCWMCITGTVRRATDGHLIHCNIDTDLVIEKPQTQNNCVPNELYTIAENFSTATKRLHIIPGRTGLETPVRLRRGWVIMSPDVMLNNFDSKKYKQEIQALGNNMPVQEDIETLRPKSPIQSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI29</accession>
    <name>A0A1E5RI29_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RI29"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="310" mass="35438" checksum="B1327A13396F620B" modified="2017-01-18" version="1">MKATSKSSDYNVSXKITSPHNLKRLLIMAHAFXSLTKAFLEHGDSLNDVIQNLKTISALFDDVEETENHETSQNENENETLEGNEKKRVLTPMRNLSLDQKQKIADALKRLQSNTKQRNYKITKLMRYYDQHFELTEKRLITDQIKKKKKKQLKLIEGEAFKIQLKLNYLLLKLVYLTSSSNLANQPVPSYQLNERSVNICNVDNPDAPVWNLISRFENLDSTSEFDEVVAEAXKITESMCNLNFVARVEKLDAAEEVNGVLAKQLSEIQNSLENIKQGNSESEKMFFAXSGNSLKSLLSQFQAINSQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI88</accession>
    <name>A0A1E5RI88_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Homoserine O-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1591</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. MetX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI88"/>
    <dbReference type="FunCoup" id="A0A1E5RI88">
        <property type="interactions" value="143"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI88"/>
    <dbReference type="InParanoid" id="A0A1E5RI88"/>
    <dbReference type="OrthoDB" id="191364at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004414">
        <property type="term" value="F:homoserine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009092">
        <property type="term" value="P:homoserine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00296">
        <property type="entry name" value="MetX_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008220">
        <property type="entry name" value="HAT_MetX-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01392">
        <property type="entry name" value="homoserO_Ac_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268">
        <property type="entry name" value="HOMOSERINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268:SF11">
        <property type="entry name" value="HOMOSERINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000443">
        <property type="entry name" value="Homoser_Ac_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="5">
        <location>
            <begin position="79"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="274"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="317"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="498"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="337"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="499"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="435"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000443-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ86303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="527" mass="59119" checksum="C6C2E9EDA9696EBE" modified="2017-01-18" version="1">MSTTTETLVDASHVIHTESASGYYEIDPSTYNDTNPFVSLVTNQKIVNVPEFVLESGYKLTNIPIAYKTWGRLNTKRDNVIIICHALTGSSDVSDWWGPLLGKTNKAFDYSKYFIICLNSMGSPYGSFSPLSINSATGYNYGPEFPLCTVRDDVRIHKQILTEYLHVNSVACVIGGSMGGMLTLEYANTFGPEFVRNFVALATSARHSAWCISWSEAQRQAIYSDPKYKNGFYDLNDPPTTGLSAARMSALLTYRSRNSFEKRFARGNETRKTVTKENPTTTTVTNTSFSNDTSDGTKTEKFYKDKVSTITLQNLKIHNDGHKPHPAHSRSIERKNSASSISSSNSSSTTISHTTHTALHTKMDTKPPQTFFSAQSYLRYQGEKFVSRFDANCYISITRKLDTHDLQRGHDDCESLEEVLQKIEQPNLIIGIESDGLFTYSEQEFLAENLPNSTLYKIDSMEGHDAFLLEFKEMNTLIQTFLADNCPVDPETLISVSEKDEEGYEEELEEGQGKPSLFGEAEEVTNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIA1</accession>
    <name>A0A1E5RIA1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mitochondrial distribution and morphology protein 35</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1343</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRIAP1/MDM35 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RIA1"/>
    <dbReference type="FunCoup" id="A0A1E5RIA1">
        <property type="interactions" value="192"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RIA1"/>
    <dbReference type="InParanoid" id="A0A1E5RIA1"/>
    <dbReference type="OrthoDB" id="19091at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990050">
        <property type="term" value="F:phosphatidic acid transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007918">
        <property type="entry name" value="MDM35_apoptosis"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46403">
        <property type="entry name" value="TP53-REGULATED INHIBITOR OF APOPTOSIS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46403:SF1">
        <property type="entry name" value="TP53-REGULATED INHIBITOR OF APOPTOSIS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05254">
        <property type="entry name" value="UPF0203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="99" mass="11297" checksum="43FCEB227CEB3363" modified="2017-01-18" version="1">MGQSISSPVPVFAKECTELHKKYDQCFSTWYDKEFLKGESMENPCLDQWTDYMQCVNVALIKKGIRSIDTFDKEDLPDNIGEVLKQHAASQESTSEKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIB1</accession>
    <name>A0A1E5RIB1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Epsin-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1185</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RIB1">
        <property type="interactions" value="159"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RIB1"/>
    <dbReference type="InParanoid" id="A0A1E5RIB1"/>
    <dbReference type="OrthoDB" id="4033880at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16992">
        <property type="entry name" value="ENTH_Ent3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000006">
        <property type="entry name" value="Clathrin interactor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF45">
        <property type="entry name" value="CLATHRIN INTERACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="2">
        <location>
            <begin position="24"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="333"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="370"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="413"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="438" mass="48598" checksum="B600887CA5901DB5" modified="2017-01-18" version="1">MNLENTLSNLSLYDAKKYFRKAQNVVFSYTEMESKVREATNNEPWGAPSTLMEYIAQGTYNYKEREEIIGMVFRRFTEKTAMEWRQIYKALQLLEYIIKHGSERFIDDCRANISLISMLKDFHYIDSQGQDQGLNVRTRAKAIVSLLKDDSQIRKERKHARETAEKYRGIGNTPSRKPNGVLNRGGGFNKTSTNGVSVSADFDDEEEGGFSKRDYSTYDSTSYGEYKEEVIDHPHKTTXSTVKSTSLXEITNSEVPDLLGEDSLFETPINANHASEVTHENEDDDDEDEFSDFHAAPTSSTSTTNTNFNLSGNLSNMYEANKPSNTANDNLFSSXSNATPXSTAPPPKAGSFPQASRKPKSDDLFGSLLTAAKAAKPTPTPTAKSNNPQSTTHTDEDDDFGXLMGGSEPFNKNSGATSTNNSHSNNTSNNGEVDLLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIH5</accession>
    <name>A0A1E5RIH5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein NRD1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1556</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RIH5"/>
    <dbReference type="FunCoup" id="A0A1E5RIH5">
        <property type="interactions" value="289"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RIH5"/>
    <dbReference type="InParanoid" id="A0A1E5RIH5"/>
    <dbReference type="OrthoDB" id="79367at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031126">
        <property type="term" value="P:sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000397">
        <property type="entry name" value="RNA binding protein Nrd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048892">
        <property type="entry name" value="Nrd1_Seb1_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36911:SF3">
        <property type="entry name" value="GATA ZINC FINGER DOMAIN-CONTAINING PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36911">
        <property type="entry name" value="LIM ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21380">
        <property type="entry name" value="Nrd1-Seb1_dom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CID" evidence="8">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="374"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="157"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="254"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="510"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="612"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="159"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="258"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="280"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="300"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="519"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="625"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="649"/>
            <end position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="665" mass="74319" checksum="8057ACB071ECBE51" modified="2017-01-18" version="1">MSDIQDFIDNLEAIKGLKSGISGSRIKKLTTFALDNVVQEDEVMNLIVDYSKTCEPSHKLGSLYIIDSIGRAFLEECKNSKRDITDNDIVGTVQHSLKTLNAHIEDLLGSAISEVSDDHKDKIRDLINIWDRAGLFIKQHLNAVRGKYLASSLDLPRPSTNISSSSSSASASTSGSSSINNKNSYNNDNDNGTGNDEDPLFESVPEHLKVEPSSFASEINFADLTSSIPEKQTDALKELLSFLQLNKITISQLPGFSNYKNFNNTNANKPGRSGISKPLNDQRRMYTSRDSRSGNNDMVNQRNNGFNNNNNNNNNNNNDNNNNSRRGGDQGNLNGNNHHLYPDEKNVPTNPHFRPKPVSTDPSIPPDCIRILSRTLFVGAVTQQYSQHDIANIIRPFGEVQSVILNHGRKHAFVKVYSRAEAVNIITNFNRSTTSPLRIRWAVGFGPRSMFDYETGVGLVPLEKLTDADKKWTLTCEWGGTNGLPLQGQMVFEEPDIVVGEGISSKAIDHKLNGDRYGNRNNNRRNNNNYNNNNNNNNHNNNNNNNFNNNNYNQRQQQGYYGGAQQQLPPQMFPGQMHGQIPGQIPGQMPGQMHGQMPGQMANPMMGQMPGQMPGQMPMYPPPQYGYNQQQSPQQQQQQQPQKFMDPNAQVSSLMSYLNQHQQPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIT7</accession>
    <name>A0A1E5RIT7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Nicotinamide mononucleotide adenylyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1640</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>beta-nicotinamide D-ribonucleotide + ATP + H(+) = diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21360"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="EC" id="2.7.7.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RIT7"/>
    <dbReference type="FunCoup" id="A0A1E5RIT7">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RIT7"/>
    <dbReference type="InParanoid" id="A0A1E5RIT7"/>
    <dbReference type="OrthoDB" id="5591297at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00600"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000309">
        <property type="term" value="F:nicotinamide-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02165">
        <property type="entry name" value="NMNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005248">
        <property type="entry name" value="NadD/NMNAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31285">
        <property type="entry name" value="NICOTINAMIDE MONONUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31285:SF0">
        <property type="entry name" value="NICOTINAMIDE MONONUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="9">
        <location>
            <begin position="37"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="252" mass="28799" checksum="36BB234BA4F380FB" modified="2017-01-18" version="1">MEKLCQKFISSAADFLVVHSNNEIKALPFSSRLLVLDSSFNPPHLGHFNLVHRAVEHFKHHGQTCSVLLQLSVNNADKAPQPASFPHRLEMMQVFAQELQSQLGCNCLVALTKHGKFLDKSEAIKEGLGRDLHIVYLLGFDTLIRLFDPKYYKPISVQESLAEFMQNTELFCLTRDCQNGEQMNWVRECENVPEQWEQKIILEHNSDSKTNSISSSNVRNIVKESPNSQDLSKFLPDSVTKYLKLHDSKNIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNF3</accession>
    <name>A0A1E5RNF3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Translation machinery-associated protein 46</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g682</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNF3"/>
    <dbReference type="FunCoup" id="A0A1E5RNF3">
        <property type="interactions" value="1389"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNF3"/>
    <dbReference type="InParanoid" id="A0A1E5RNF3"/>
    <dbReference type="OrthoDB" id="278280at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032378">
        <property type="entry name" value="ZC3H15/TMA46_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681:SF0">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681">
        <property type="entry name" value="ZINC FINGER-CONTAINING PROTEIN P48ZNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16543">
        <property type="entry name" value="DFRP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="157"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="157"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="336" mass="38847" checksum="AF7BE14AB633CEBA" modified="2017-01-18" version="1">MPPKRAQKEPKKKKDNVDKTFGMKNKNKSTKVQKFIKQVELQADPKKEELRQKKLKEKQLREAAEAEKRALLGQTDTHQRVRPGVDPKSILCAMFKIGNCNKGAKCKFSHDMTIARKVEKIDLFSDARKEKEQDTMDQWDEAKLRSVILSKHGNLKTTTDKICKFFLKAIEVDNNYGWFWECPNGRTCMYKHSLPEGYVIKTAEQKKLEMEALENQPKITLENFIETEREKLDKSNLTPITKENFREWSLQNDIAKINIKVSQVVKKTGREVVLMKMHEDKKWAEDLNFEADKGTAFDLAAMERASQLEKAQEAEANGEAQLNIKDYGDGSNPIFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNJ9</accession>
    <name>A0A1E5RNJ9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g827</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNJ9"/>
    <dbReference type="FunCoup" id="A0A1E5RNJ9">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNJ9"/>
    <dbReference type="InParanoid" id="A0A1E5RNJ9"/>
    <dbReference type="OrthoDB" id="3994490at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018555">
        <property type="entry name" value="C630.06c-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09428">
        <property type="entry name" value="DUF2011"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="213" mass="24397" checksum="B6E1F7E2395F1975" modified="2017-01-18" version="1">MTENTNEKPYALPDLDLEFVVVDAPSNESVDASNAHPQEEYDFPLFSMGNASNSLNKDENFSATEPQLIKVSLKDDAAELDSAISKINTRPQSYYFKHFTELEKLQIKQSCLEPNDIIGNAHFLWNTAWPQFNAPVIDLAEYNNKVEQAQIKMKKLSNRKPGQRQRISRREAKQRVLEREEKRLSLKKKFRKRGGKKNKKISPLAHAGKKPAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNS5</accession>
    <name>A0A1E5RNS5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcription factor TFIIIB component B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g856</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNS5"/>
    <dbReference type="FunCoup" id="A0A1E5RNS5">
        <property type="interactions" value="119"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNS5"/>
    <dbReference type="InParanoid" id="A0A1E5RNS5"/>
    <dbReference type="OrthoDB" id="272624at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000126">
        <property type="term" value="C:transcription factor TFIIIB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000995">
        <property type="term" value="F:RNA polymerase III general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001156">
        <property type="term" value="F:TFIIIC-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070898">
        <property type="term" value="P:RNA polymerase III preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017174">
        <property type="entry name" value="Bdp1_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039467">
        <property type="entry name" value="TFIIIB_B''_Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22929">
        <property type="entry name" value="RNA POLYMERASE III TRANSCRIPTION INITIATION FACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22929:SF0">
        <property type="entry name" value="TRANSCRIPTION FACTOR TFIIIB COMPONENT B'' HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15963">
        <property type="entry name" value="Myb_DNA-bind_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037327">
        <property type="entry name" value="TFIIIB_Bdp1_fun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SANT" evidence="3">
        <location>
            <begin position="425"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="193"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="306"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="505"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="50"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="105"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="195"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="217"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ88353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="600" mass="67742" checksum="F454A3A1E8D17CB2" modified="2017-01-18" version="1">MSSVVNKSGTRFVPKIKQRRAVSQPKQDKSKDNDQNLAKPISANAEATEDINKDSKSIEEIAETHLESPGPSRSISPFSKSGPLDGQVPGEKNAESFSANQPADGSEKPDSFETSDKNSDSLNQTEAVQQPRRASVRLDSISSTRRMSVGNRMSNGNLSSQMQQGRLVSMVKKNPQLLSANNDKQLQAMKRRRLSSMSQKSSFTIPNSNKVSKPAKRISIISRVSTSSEDISDGSRKPSIAPNGGDDDGEDLEQKKYRISTVADIPKELTEDEGIKYHLDDSTFTMAELCKPVLPIGAISDNYHIAQEATKKKLEEKKKRRALRKLAREQFKPLKLLTKEEDELRFEERRLKQQELLNAEVPEEEQQPQQQLQLKIGADGNIILDEESTVVDRHANANLVNMHKERQDENTFANLYNTASYSKREYTDPWTPDEEVKLYKALSMWGTDFNLISQLFPYRTRRQVKLKFSLEERRSPVLVELALKRKLPPDFEKFCLEIKQDIGTIEEFNSKLQGLKVKHDEHLKQIQLEKETAKQEDDRKIQENIQNGANGGPFVIGSLDDRKLAGGFRESDLKSYRKSEIVLGSIDDVKRSTTDHEAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP34</accession>
    <name>A0A1E5RP34_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Polynucleotide 3'-phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g935</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP34"/>
    <dbReference type="FunCoup" id="A0A1E5RP34">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP34"/>
    <dbReference type="InParanoid" id="A0A1E5RP34"/>
    <dbReference type="OrthoDB" id="19045at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046404">
        <property type="term" value="F:ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046403">
        <property type="term" value="F:polynucleotide 3'-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013954">
        <property type="entry name" value="PNK3P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12083">
        <property type="entry name" value="BIFUNCTIONAL POLYNUCLEOTIDE PHOSPHATASE/KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12083:SF9">
        <property type="entry name" value="BIFUNCTIONAL POLYNUCLEOTIDE PHOSPHATASE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08645">
        <property type="entry name" value="PNK3P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ88651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="294" mass="33189" checksum="1A9E8196041F20FC" modified="2017-01-18" version="1">MSRPIGKHHKLVLLPHLIKIIPNKLAEHATFHNGSKFYGFDLDSTLIETKSKMKFSKNPKDWKFVELSDPESSGKESVTTLDALLQLFKENSAKAQQANIVIFSNQGSVITVPGDSKSCTNLLSKLQNILEVIENSLREQDSLEFMNHIWVYCSPKMPSSLKKKKVFKIGGANSLISAKFKTVSNSANKITKKQQHTEQETTMETKSQLSQELLFAIMRKPEIGMFETFQQDVHKCFLSEKDASKDIALEFYCGDAAGRPTDFSDSDFQFAKALKTQFLTPEKFITEYTDKKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP38</accession>
    <name>A0A1E5RP38_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">diacylglycerol cholinephosphotransferase</fullName>
            <ecNumber evidence="12">2.7.8.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g738</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>CDP-N,N-dimethylethanolamine + a 1,2-diacyl-sn-glycerol = a 1,2-diacyl-sn-glycero-3-phospho-N,N-dimethylethanolamine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33775"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:64572"/>
            <dbReference type="ChEBI" id="CHEBI:65117"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:33776"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.2" evidence="12"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP38"/>
    <dbReference type="FunCoup" id="A0A1E5RP38">
        <property type="interactions" value="531"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP38"/>
    <dbReference type="InParanoid" id="A0A1E5RP38"/>
    <dbReference type="OrthoDB" id="196717at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004142">
        <property type="term" value="F:diacylglycerol cholinephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000012">
        <property type="entry name" value="sn-1,2-diacylglycerol cholinephosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014472">
        <property type="entry name" value="CHOPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414:SF37">
        <property type="entry name" value="BB IN A BOXCAR, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414">
        <property type="entry name" value="ETHANOLAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015665">
        <property type="entry name" value="CHOPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="130"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="177"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="254"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="286"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="320"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="348"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ88655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="388" mass="43945" checksum="E8E3E9F512AA74DF" modified="2017-01-18" version="1">MGYYVPHQCLPNLKKYKYSSEDRSLISKNILKPFWGQFVKIFPLWMAPNVITLLGFGFIVVNFLTCLYFDPTLTKEHPRWAYFSYAFGLFMYQTFDACDGGHARRTGQSGPLGELFDHCIDSLNTTVSMLIFCAVSQLGFTFPAIFAQFALLCNFYLSTWEEFHTHTLFLSEFSGPVEGILLIITSFILTGILGPQFVWHYPIAKIAGLQIESFHVLFGLFGLALLFNIYYAQKNVADYYQKNKLEAHMNDPELAIASARAGLIPFFAFFAVVFVLCLIEPQFISLPFVLSNGLAIAFTVGRIIVAHLTMQDYPWKNSPLFLPLAQLIAYQFFTSVVGYNASTVCTNLCWMGFGLALGVHATFLNDIIFDFTSYLDIYALSIKHPKIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP46</accession>
    <name>A0A1E5RP46_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Morphogenetic regulator of filamentous growth protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g604</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP46"/>
    <dbReference type="InParanoid" id="A0A1E5RP46"/>
    <dbReference type="OrthoDB" id="774557at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051241">
        <property type="entry name" value="DZIP_RILPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029005">
        <property type="entry name" value="LIM-bd/SEUSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21502:SF3">
        <property type="entry name" value="CILIUM ASSEMBLY PROTEIN DZIP1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21502">
        <property type="entry name" value="ZINC FINGER PROTEIN DZIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01803">
        <property type="entry name" value="LIM_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="63"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="133"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="264"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="548"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="655"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="31"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="70"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="141"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="283"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="554"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="665"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="706"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="762"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="794"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="841"/>
            <end position="860"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="871" mass="99343" checksum="56F8A22AF28EA5B7" modified="2017-01-18" version="1">MGGTAGRLPDNGEQTQNTTPHAGSFNDNESFDPRSSDNLRAHQDYDANYGQFSYKGHKDQEKMVVAGSHLQPQQQTYYSNAQQTIEHSNQQNLSNAHQQQRQKYQHQQHQQHQQQQQQQQQQQQQQQFYQQHQYQQQAQQTKMPQHSSSTIPVPRANRVTKNTAPQQLPVYRTMVQQNSTKLSPQVQQQLQQQIKQQQQQIQQKQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQXQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQYSTNPNVAARDVPGVNRNSNINSNEAASKPYPDNSQSQHPKNQAKAKQESKQSLQNIPSHMLGLLNPENAAKASLVHPESLTNQPPLPYPVRKYFANMAILRVHEIINMLLMVRMGSNGKDPQGLTNFVNETFAPYCNIKYTVRNQAKEIRTYDLSVLVFADFLKNWLSSCTVRVEIVPQQLRSQVLNNGTIMFECPCCTFTRYFDDGSYITRFFQMKGIFDSWLKLEWLDLNGHSFITGVEWGSIERLLLNPREKNDILYSMISKEGVPEEESAIGEYSLEAETGQTKKESQKKSKVKSEKNNGPMPPGGSASSMNESIEKLRSKFEIFQNLNSFGTTESFMKIMQVNDVMQHLKSLMLYQRLNNVNSPLEAMYELVNNMTLQQKQAAEANKKSAPAFSQTSPTPSVFQQQPSNNFAGATPQNSPPPGYLATQFPGASAQTVPQNFHSQKSPQDQYFSGQHTPQEPLKNYQNNPPYTSNTPSTEPTVKQYAGNPEQTNNSYIQSARPNPKTGHAPSAPYKEALHPSQMQSYQNKYYEQSTQTHAGISSTTNLNDPSFGPAAGSSEPSVMPVPQNRSPSGLSPHSQVQINYNADTLEPNNPKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV34</accession>
    <name>A0A1E5RV34_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g400</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RV34"/>
    <dbReference type="OrthoDB" id="6067455at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ90800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="293" mass="32565" checksum="6FCE07194190008B" modified="2017-01-18" version="1">MSTFNSRFSNLDVYCFDSLASFSNTDNIKAHRPKIKASTNPYGSVLNDEISPKNSLDFSVLKSIDCVPNXEXTSCSLSNALYPHAHFPIPSKNGLDHGCSIQTSLCEQGXDLHSVSSCVSGGVXHENILSXLYFDPRADEKGEENKMLEILCHEMNSESPLVSGFFKVLSEQNQXIEKSVKWSDYVFYYIXGTDRTGYFEIQEKIKQVQQSSWCVFDKXFWDNCVEDTXSASSSLASPITNFIKSDGXPLHDSKSRCNNNQSSSDKTIAQSIDIVSVDSCSSGDFCCRVFXXV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV67</accession>
    <name>A0A1E5RV67_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">40S ribosomal protein S13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g466</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RV67"/>
    <dbReference type="FunCoup" id="A0A1E5RV67">
        <property type="interactions" value="984"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV67"/>
    <dbReference type="InParanoid" id="A0A1E5RV67"/>
    <dbReference type="OrthoDB" id="623277at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.10:FF:000003">
        <property type="entry name" value="40S ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.130:FF:000001">
        <property type="entry name" value="40S ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_uS15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023029">
        <property type="entry name" value="Ribosomal_uS15_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012606">
        <property type="entry name" value="Ribosomal_uS15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006331">
        <property type="entry name" value="PRK08561.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885">
        <property type="entry name" value="RIBOSOMAL PROTEIN S15P/S13E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885:SF6">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08069">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01386">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00362">
        <property type="entry name" value="RIBOSOMAL_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS15 N-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ90831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="141" mass="15948" checksum="3B9BA77D3F24B2D1" modified="2017-01-18" version="1">MSSSALPYSRAVPSWFKLSSDAVIEQVIKYARKGLTPSQIGVLLRDAHGVTQSKIVTGNKVLRILKSNGLAPEIPEDLYYLIKKAVSVRKHLERNRKDKDAKFRLILIESRIHRLARYYRTVAVLPPNWKYESATASTLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV79</accession>
    <name>A0A1E5RV79_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">pH-response regulator protein palF/RIM8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g519</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RV79">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV79"/>
    <dbReference type="InParanoid" id="A0A1E5RV79"/>
    <dbReference type="OrthoDB" id="7785529at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF161">
        <property type="entry name" value="PH-RESPONSE REGULATOR PROTEIN PALF_RIM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="2">
        <location>
            <begin position="409"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="707"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="711"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="728"/>
            <end position="739"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ90842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="739" mass="83081" checksum="01A6D6082FF502AA" modified="2017-01-18" version="1">MKDFISKIKTSILPSNKENFAETNLQLKNKTTKPQIPVKTGLPQKSRTSVKSLNVFSSKHKIKDGQSSLAFNGLPADISKSKELKPEIKIRIVNQPLAYEPGDYIEGEVHLKLKCNVTNLQLDLTFQGIVTLRGANKKQGISLVQHSPLNSDHESKSGLTLHANKSSGTINTNSTHNGTKSEMEINRDDSDKNVAQKKTDVXVNHIEGXNSNVFVLFDKQIFLYGEDTTGLDDINTPIINGLTSGEHIFPFKIRIPSGRNKSVYSSISFEKGSIRYNLKSSLYTIFGLPSDNKTDETTNNDKAQSQLVDAQELPTNSLLQSITKANASFNANKKXVTTSSKEVFVIEPIDVSPYITPVKREIVLGSPEGLSQNEPNKTKAENIAIDETTIDLKSGNHVSEVGKKISAGHSKIVKFEIELDHKGYRLGDKINMKFRLQHYKDFVYPVGVIATLLRICEVDNAQEFYRKDICQTTIPIATTPVPVSCNTEPGEELFNHQYEQNLSLKVPLDILPSLNMKECKFFQFKYYVELTVNLSQKNLVMTENNHVLCGKYAPRTTSHYKREEMKFKNYQSVFSKNNKDKRLKELIALQKKVLSKRTVSDDYTVDNPLECYFYQDLVDVAKLNKGKNVTSLMTEIVVGTFRKNTPNVENSEINSLTNNVTTPTVELRTAVEDVYDQLARNITDTDIGLPDSYPFWSTRDQLPKYSAPAHASHDAFDVSDNKEELEKQTLQNLESEPPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVE2</accession>
    <name>A0A1E5RVE2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g474</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVE2">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVE2"/>
    <dbReference type="InParanoid" id="A0A1E5RVE2"/>
    <dbReference type="OrthoDB" id="2565191at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022793">
        <property type="entry name" value="Rrn10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05234">
        <property type="entry name" value="UAF_Rrn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="239" mass="27753" checksum="F9227CDF8FAEF0B7" modified="2017-01-18" version="1">MNRSVFEVCNDALLLNNQHTFKSFDEVLFDTLNAXGVGVGXDENGEKNKKGGKNSDEIHQHRLIPLPEPVRDGELDKDSIDMTVLHYYLAKEILDKYPFLINKFDETSLLMIRMCIEQWIDEYLIKDEELEDVGRDRQGYLDVEMMNEYAQAQRRKGXSVEISDIEELVFDGSMEKTKSKEKTKSKEXTKSKEKTKSKXXTXSKEKXKTKSSIEKKKKKKKKKNEQENKKAKSLRAMVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVE3</accession>
    <name>A0A1E5RVE3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein STE5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g405</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVE3">
        <property type="interactions" value="94"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RVE3"/>
    <dbReference type="OrthoDB" id="3972734at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11070">
        <property type="entry name" value="Protein Ste5, Fus3-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038382">
        <property type="entry name" value="Ste5_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021106">
        <property type="entry name" value="Ste5_Fus3-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12194">
        <property type="entry name" value="Ste5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein Ste5 Fus3-binding" evidence="3">
        <location>
            <begin position="502"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="55"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="384"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="4">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ90795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="649" mass="73164" checksum="45A80BA60934F6E2" modified="2017-01-18" version="1">MSYSVLQEAHITYLTNSHGTVDSTGHATPPRTPVLQSSHIFSSPISKLINFKKKSSNASTSSXSSSTTASPQTYXSNNATPKKITRLTDDYIFGSPKPAQKTLPQKQSVREXLYXKADPSIYSTQQQMFHPSPKRPPPPRPIADGISKENLSLSNDFAKTQTANTNINKEGQNGMPKKRYVNEECCLCFEKLSTRSKNEKVFSAKCGHVVHEDCLLLFIENTVRTENDTNGEMQRGNLFPLCIKCTETNKSNRCIPIDSSFQDNLLSRLLLNSKEYDTNDKHESHPSTMQNTFVQSVPPLSLVPQKKYTSVISPIVQGQYPQYLSRYSSQKSKKSLNALNSPKPITRKVAATTPTLVSSRIRTSAAFVNAKKHTTVTSTVVGFNTILNTANEQYEEEHEDEELEIIHIDDLHQLHELHELHELHELQQPAESVRQVVVRKVKVPPKSLXIVLQLDTHKKIKFAENXSIVNSLKALDIWYGYMNSKEQKXSFQYDNDGVPWSVKLCCIDETGKVLSSGQISDVLKQLGSTPGLQFSQIRGKMLTSDYWDQTLIQXYYQDSDTPIEVCIISNCGVXNSTGILSNPSTNTKTNIMMWNNFFTLTKETLCKIHIGYLNEDIGDELIEINNWTEIMELLCVYYGFEFGQDFEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVG0</accession>
    <name>A0A1E5RVG0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative tyrosine-protein phosphatase OCA6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVG0">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVG0"/>
    <dbReference type="InParanoid" id="A0A1E5RVG0"/>
    <dbReference type="OrthoDB" id="6375174at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004861">
        <property type="entry name" value="Siw14-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF14">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE OCA6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03162">
        <property type="entry name" value="Y_phosphatase2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="263" mass="30496" checksum="36ECADF58FC6B8F9" modified="2017-01-18" version="1">MFDSAIYTTPLNFSQFQPKLYRGTQPHKKNFPYLKLLQLKTIISILPEPITEENDPELVKFMQVEKINHVHIPCDGKKKKKKKKDKNKKNTEKEKVKEETKDGKEKDKEDKSVTEISGIDTADAKKSKKLQDGKDSAKQESSKTLKKEEEEKKVKRRDKAVPITEQSVYEIIDILLDNRNYPVYVHCATGETITPIVVACLRKLSYWSSVSIVDEFIRFSGSINVHERAFIEEFNVDDFTPKLTDKTLYASWIKRTQGWXTKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVH4</accession>
    <name>A0A1E5RVH4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Palmitoyltransferase</fullName>
            <ecNumber evidence="12">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g368</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DHHC palmitoyltransferase family. SWF1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="12"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVH4">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVH4"/>
    <dbReference type="InParanoid" id="A0A1E5RVH4"/>
    <dbReference type="OrthoDB" id="9909019at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF489">
        <property type="entry name" value="PALMITOYLTRANSFERASE SWF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 12 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="14">
        <location>
            <begin position="27"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ90942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="249" mass="29400" checksum="85627D7B3D18B794" modified="2017-01-18" version="1">MSLFQXLDHSEITESPWSYDELIFYSTNNVCSTCETIKQPRSKHCSICNVCVSFQDHHCIWFNCCISGTNYKWFYMFLVSNWFILAYGALRLTYIKYGQTKIAIPQYYQNSVLTLIILCFCFVILLSWFLYTQWELIKEGMTTNECDKWYTVHQLIEDGLLVRSTKSNKYYELYNTNDHGKQNSFHGNNNNNNNNDNNTKDVYGISLNPNDNSIYNLGQMFEIVQSPYEIINIYDKGSFWNNLKERLNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVK3</accession>
    <name>A0A1E5RVK3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein transport protein SEC9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g534</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNAP-25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVK3">
        <property type="interactions" value="81"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVK3"/>
    <dbReference type="InParanoid" id="A0A1E5RVK3"/>
    <dbReference type="OrthoDB" id="3972224at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15857">
        <property type="entry name" value="SNARE_SEC9C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15886">
        <property type="entry name" value="SNARE_SEC9N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305">
        <property type="entry name" value="SYNAPTOSOMAL ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305:SF9">
        <property type="entry name" value="SYNAPTOSOMAL-ASSOCIATED PROTEIN 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="3">
        <location>
            <begin position="682"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="53"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="151"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="175"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="288"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="319"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="371"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="420"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="430"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ90879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="745" mass="83017" checksum="8D82AA2F7AB6B0FB" modified="2017-01-18" version="1">MGIKKIFGIKPPEEMTDEQNREYLMDLGVPVKNLNKKKRSKFAAYGAMGSDRRFEKTYAPPGYEEYAKPGNATVNAEDDEDDLNKSVADYKLDDDSSKSDSNNPYSRNNTGANPYDRGAGGAESVVPFSAVPARETTSNPYSRGSDPRATNFGNTYESKNSAHPNSYERAPQGNPYGTASQSNPYAGVTNNDFYGKSSQTNPYGNTETPSAYDSSYNADANAGGQNRVESPYGASTSGPASNAQSSFSAGDSAELEKGSDFAPSAYSPRPGKGVRNSNNPFTRRATXNADTPESNFGSSSPALGRNPSQRANRIVSPRGSXNPNRQSTLMHNNSSSNPYANMSSNVYNTNGNSNTSSSATRTSSLKKRNSMSHPYNNEGSYSDVDTFDFEDPSVSTPPGNRRDGEGNHNNYENVSGEFGSSNNDSNYNNEYGQSNYKSNLGNTYNGDDDDDFNAAPKQYYANDDLNASIYENEEYANNGANEFGSYQGGSYNEGNRGYKTFEELREEEQMKQQQMEDEEVDEIKQNIRFTKQSSVASTRNTLKMAQDAEMAGMNTLGMLGNQSEKLNNIESNLSLMKVQNRTATHKVDDLKKYNRSVFAVHVGNPFTSKRRLREKEEQIKQQRMQDKLMQEHVDQELYSSVKRIGGAMDEVTKTDELKDRYQRREILEKAKRYQFEQDEEDDDMEHELDKNLNHISDISGRLRKLAIATGQEVDKQQGRLREIEDDTDNLDINIHLNTHRLHNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVK9</accession>
    <name>A0A1E5RVK9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g372</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVK9">
        <property type="interactions" value="293"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVK9"/>
    <dbReference type="InParanoid" id="A0A1E5RVK9"/>
    <dbReference type="OrthoDB" id="1928777at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14077">
        <property type="entry name" value="STKc_Kin1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028375">
        <property type="entry name" value="KA1/Ssp2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001772">
        <property type="entry name" value="KA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02149">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103243">
        <property type="entry name" value="KA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50032">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="298"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="KA1" evidence="14">
        <location>
            <begin position="1460"/>
            <end position="1509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="243"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="839"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="921"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="972"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1041"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1248"/>
            <end position="1286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1304"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1393"/>
            <end position="1430"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="768"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="19"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="44"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="59"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="98"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="111"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="840"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="863"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="929"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="980"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1043"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1076"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1102"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1121"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1248"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1276"/>
            <end position="1286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1320"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1399"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1411"/>
            <end position="1430"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ90977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1509" mass="169228" checksum="98261AD5BD320362" modified="2017-01-18" version="1">MSEYNQYNRFDNSTANNTQEQGHGQGQAQAQHSQQDYGLKSPIRGSNTSSNPTSNDYMNTNPTNAYASYNNNTTTGNNNNYNNTHNYNYSNNPRTVLASPQRSGSGSTYYSTNNNNNSSNNNNNNTHNNNNSNNNKNNHENYVPSVAPATPRMLRSHSRNVSNTTNMNNINNNQNNLDFTYDQQLQQQQLQMKMQMQYGNDYENNAINTPKRNQQDGNVVDIATGVSNNNNDYLMPPLPLQQEQTIQQPQQPQQPQQSQQAHHAHHHQAQNTHHLDHPVTPKPRKQPKEFHRTSLGDWEFIENIGSGSMGKVKVAKHKYTNEVCAVKIVTRAVKTFLYKHDQYQAYKNKDTKKQFDEKLKKEASRDKRTVREASLGQILYHPHICRLFEMCTMTTHFYMLFEYVKGGQLLDYIIQHGSLKEKHARKFARGIASALQYLHMNNIVHRDLKIENIMISKTGEIKLIDFGLSNFYNYSSKLHTFCGSLYFAAPELLMAKPYTGPEVDVWSFGVVLYVLVCGKVPFDDENASVLHAKIKEGKVEYPNHLSIDSIALLSRMLVVDPLKRASLQQVICHQWMNKNYDFITPSYLPYREPIQRIDKTVVKEMCRLELYEDVEQTYYELLALIGSPEYTEMAKEYYNFNPPLISGSAKNNDVHNNYTYAPNNFDTASSDVSMKGDSPFGNGNLTESAYASASASSMNSLNSSAFPSQHQLHNKDKTPLVNTNASLVPKTGSSVSLETTSKLGTVEIDPTRAFHPMISTYYLVFEMMERKKLKLRKRNEQALKLQQQQQQQLQMQMQMQLQQHGQLHVQKSPLKLTPNQQHPASPSTVLPKLKIPEQAHTPNIHSPTSSRKVSNDLLSPMEQSSQHRQQPQQHHQIEQQHNQLTNSSLLSDRNYMHVPQQHAPVEKSKIGSIFRRFSQXKPEEEEAYSTATPTGGQAYASSAKTGNTAGGENKKVWRKTHQRTVSEYVPPSRIHSYGATPTTTXTKTTTGQPPVIDLNTNVPLQSSTLNTSAFPALPANADELVKQQRMKEMNKKDDLKLDNVTNNSTSSEFVNPNNNPVFTTGPAQNGEQPRTVTRRMHPSARAKSVGHVRKESLTFLRPSSGGSNAPSGPNDTPKLAPPAQSTAASANPAPATNEISKQNTHQHYDENSAVGGNDKATTHFYKKMTEDEILEEASKAPMGTMPSIDYPRTLFLKGFFSVQTTSSKPLPIVRYEIISTLTKMNIQFKEVKGGFECVYKNQNTSMLNSSKVTKPTGSKTPHNYENQTPLATSTVSDDENDDYDTDDRELDYITDAYAYSNNDAESGSFVRKNSDEDTGKQNVSRHGSVVKSNYKSQIPATPKASANAQNYMAFRSPLPAQHQQHIRQVSGVDELSTASLKEDEANQLHNMDGLLTTNSAGDGAHSSDASQPEQLQFSSEPASTNNTATGVSDVDQKHAFNIAEVTDVENEFKTVADAADSISKSPIKFEIHIVKVRIIGLAGVHFKKVSGNTWIYKKLATHILKDLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVL5</accession>
    <name>A0A1E5RVL5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peroxin-7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g339</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the WD repeat peroxin-7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVL5"/>
    <dbReference type="FunCoup" id="A0A1E5RVL5">
        <property type="interactions" value="453"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVL5"/>
    <dbReference type="InParanoid" id="A0A1E5RVL5"/>
    <dbReference type="OrthoDB" id="273771at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005053">
        <property type="term" value="F:peroxisome matrix targeting signal-2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059104">
        <property type="entry name" value="Beta-prop_EIPR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044536">
        <property type="entry name" value="PEX7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46027">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46027:SF1">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23609">
        <property type="entry name" value="Beta-prop_EIPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="100"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="EIPR1-like beta-propeller" evidence="12">
        <location>
            <begin position="148"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="254"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ90875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="332" mass="37420" checksum="8F99CA932BEDABE4" modified="2017-01-18" version="1">MLRYSTPQFSGSSVQYSPYFDNKIAVGSGANYGLVGNGKLSLLDIQPNGSLLLTNAFLTQDNVFDISWSEMNENQIITAQGDGTIRLFDINLSKYPLQVIKAHEKEVFSCNWNLVNKQNFVSSSWDGTCKIWDPERGQCVANWMPASKTLENDIHLQNKATCVHQAMFSPHDPNLVISCSSTSHITLKDVRSANQTSIIGHHGLEALSVDFNKYQPFIFCSGGADKLVRVWDYRMMSSESSLVHRNRRKPLHDHVGHRLAVKKVAWSPHCDDVIMSTSYDTTVKIWGKNRVRKTFRHHTEFTTGCDWSLWGAPGFVATCGWDGNIFVWNALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVM9</accession>
    <name>A0A1E5RVM9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Structural maintenance of chromosomes protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g394</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMC family. SMC4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVM9">
        <property type="interactions" value="886"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVM9"/>
    <dbReference type="InParanoid" id="A0A1E5RVM9"/>
    <dbReference type="OrthoDB" id="5575062at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000481">
        <property type="entry name" value="Structural maintenance of chromosomes 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000585">
        <property type="entry name" value="Structural maintenance of chromosomes 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937:SF172">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="14">
        <location>
            <begin position="774"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="58"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1226"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="421"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="495"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="947"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="1091"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="1269"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="95"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="141"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="15">
        <location>
            <position position="949"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ91002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1492" mass="169275" checksum="9E19BC222EBF453A" modified="2017-01-18" version="1">MSFSTPTKKKRKSDEKVTTPPNISDEGESSSLSSAVQRGHPADISIGTIISSPYKKNIGLHHARSPPRSNSPERNSRKLKLSNRHTISSDGTATSNGSNGSNVLSLSQQTELLPPTLNNDDMSDGEGLDGLRSDHRGRGSRPTTSTSKTSNGSLSPYRRMSPTHSPMRYSQQLHQRQSQSQSQSLLSQQSQITESQKMELIQLSPNKLKFKMQQLLHSTKQQQSLTESHGKRLYISQLVLTNFKSYAGKQIVGPFHENFTAVVGPNGSGKSNVIDSMLFVFGFRANKLRQGKLSELIHKSETFPNLDFCSVDVHFKYKYSKQSVSAADEATFPAPKDTSFVQDLVVTRKAYKNNTSKYYINGCESTYKEVTDLLRKEGIDLDHKRFLILQGEVESISQMKPKAEKEGEDGVLEYLEDIIGTAQYKEAIENSLQEIEQLNDVCMEKDNRLKIVQTEKDSLESAKDEALEFVQNENILAFNKSKLYQYKIHKSGIKLQNSTDEYSKVKAELEEETSKYESYQKDLDALKQICTSYETQLTKINEECEKLKKTQTLLNSSKISQEEKLKSFQNKKRKAETVLENVQKTIAQLEDTISDLHNEQLNMDEQLSLTSKELKPEKHKLDEMKLKLKEETVDLQRQIETIEHDLEPWVLKIQEIETSITLKQNEIEIMEQAKSNNQQRIQHFETKITESKDKIESNDAKMKKLNKELSAIRKGFSAGEKEVTKANSRLNEMKAVLNAQRQKTLEARSFLATADNQNKVLSALSXLQKAGNIXGFYGRLGDLAVIDEKYSIAISTACPRLNDIVVESVECGQQCIDYLRRNRLGYARFILLDKLRNFNLNPIQTPQNTPRLFDLIKTSNHKFLPAFYSVLRDTLVASNLQHANTVAYSTSKRYRVVTLDGKMIDISGTMSGGGKQVSKDLMRTVKSKDTXSSXGSQTVTLFTQEELSDLERNLTSKEKSFEEASTAVYEMTLELNKLQERIPDIETELSKLKFENETLSTEKELIKSQIEGVRKSLENNPEAEKELQEAQTALEGLKDSLKNTTKQTHTQNEEIEKLKKEILKVGGLDYQLQISKVDSYMQKVSLLTMKLKKSKTSIKKKETELKRAKTATKDAEKDMTKASDELEKIETNLVIISTDLRNADESLESLLNEKDDIKIKYEDAKEELDLKLSKSMDFKSLEVELNNKLSNLSKVVESTRRTIEKSKANLQNLKIHDLTSLSSQLNDEEHIQSGQSEPXELQNEVSNKEQDEMDVDSDLPDGLKQLTISELEAVNIESLQQEIEVLEENLKATNVNTDVLVEYISRLQDYRFRKTELESAVNSRDTVREKCEALKKKRLDEFMEGFSIISLTLKEMYQLITMGGNAELELVDSLDPFSEGVLFSVMPPKKSWRNISNLSGGEKTLSSLALVFALHKYKPTPIYIMDEIDAALDFRNVSIVANYIKDRTKDAQFIVISLRNNMFELSQQLVGIYKNENMTKSTTIKNIDLVHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RYQ3</accession>
    <name>A0A1E5RYQ3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Prefoldin subunit 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g252</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000002">
        <property type="protein sequence ID" value="OEJ92130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RYQ3"/>
    <dbReference type="FunCoup" id="A0A1E5RYQ3">
        <property type="interactions" value="1026"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RYQ3"/>
    <dbReference type="InParanoid" id="A0A1E5RYQ3"/>
    <dbReference type="OrthoDB" id="10267474at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990113">
        <property type="term" value="P:RNA polymerase I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990114">
        <property type="term" value="P:RNA polymerase II core complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990115">
        <property type="term" value="P:RNA polymerase III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23157">
        <property type="entry name" value="Prefoldin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000004">
        <property type="entry name" value="Probable prefoldin subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011599">
        <property type="entry name" value="PFD_alpha_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00293">
        <property type="entry name" value="prefoldin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12674">
        <property type="entry name" value="PREFOLDIN SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12674:SF2">
        <property type="entry name" value="PREFOLDIN SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="5"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ92130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="154" mass="17234" checksum="52772A7C4C575D94" modified="2017-01-18" version="1">MSQTIDLSKLNLEQLSLVKQQLEQEVQHFTQSLQSLNIVKDKFNDCIDDIKMISGSEMENTPLLVPLSNSLYVPGKVRDSQKFLVDIGTGYFVEKTSAEALLFYQKKIDKLNKESVQITNIVKEKQTAALSIDSRIRQVAMAANASQQSQPQGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZL8</accession>
    <name>A0A1E5RZL8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mitochondrial peculiar membrane protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g50</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RZL8"/>
    <dbReference type="OrthoDB" id="4044171at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035187">
        <property type="entry name" value="Mpm1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17234">
        <property type="entry name" value="MPM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="325" mass="36328" checksum="93CE0ABB0B80FB3E" modified="2017-01-18" version="1">MGLFSGDNNDKNDQKLLEQVAKQEKSTSLVPNKPEDTGLAVDGYIEGILNASEGKQPNEKTKKLVSDWWNHTTDLYGTLMEQQNVKINDLVNKTYDEAMEVTNDIIGSVLDSLGVPGNFSNPYKIQTMLDNEFPPSLDSAETPATGKNLASLTFQPKSSAEGKTNSLYAFRNPSDTQFAQCLSAKGLGVWDPNGFWRCLFPKTYIKEANLGQNELSKEDVEQDKEHRLGIFFKDYTGYLSWRNHMMNIQSQSSSALSTDMAEPNDNILGRXSSMSYTTDENGKKELTETKIFYKDGTKIVKESKFFPADGSDVVQSKEENFVSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZU9</accession>
    <name>A0A1E5RZU9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Target of rapamycin complex subunit LST8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g124</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat LST8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZU9">
        <property type="interactions" value="634"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZU9"/>
    <dbReference type="InParanoid" id="A0A1E5RZU9"/>
    <dbReference type="OrthoDB" id="400at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098588">
        <property type="term" value="C:bounding membrane of organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000339">
        <property type="entry name" value="WD-repeat protein pop3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037588">
        <property type="entry name" value="MLST8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19842">
        <property type="entry name" value="G BETA-LIKE PROTEIN GBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19842:SF0">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX SUBUNIT LST8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="71"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="119"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="204"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="249"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ92278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="306" mass="34083" checksum="182DE323C19E4D6E" modified="2017-01-18" version="1">MSVLLVSAGYDHTIRFWEALTGVCSRTIQHPDSQVNRIEITNDKKYLAAAGNPTVRLYDITSTNPNPVTSFEGHKGNITSIQFQQDNRWMVSSSEDGTIKVWDVRAPSVQRNYKTGSPVNEVVIHPNQGELLSCDQDGKLKIWDLSGNKCSTTIVPEDVDTPLQSLSVASDGSMLIAGNNKGSCYVWELPNHTDATTLNPVTKFXSHTKYITKVLISQDSRHLATCSADHTARIWDVSDKQNIQLESTLDGHQRWVWDCAFSADSAYLVTSCSDHYVRLWDLSTKEVVRQYNGHNKGVICVALNDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S0I4</accession>
    <name>A0A1E5S0I4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">Vacuolar protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g108</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 10">Functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Vacuole membrane</location>
            <topology evidence="1 10">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the beta-catenin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5S0I4"/>
    <dbReference type="FunCoup" id="A0A1E5S0I4">
        <property type="interactions" value="141"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S0I4"/>
    <dbReference type="InParanoid" id="A0A1E5S0I4"/>
    <dbReference type="OrthoDB" id="7537227at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071562">
        <property type="term" value="P:nucleus-vacuole junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000011">
        <property type="term" value="P:vacuole inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000131">
        <property type="entry name" value="Vacuolar protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045156">
        <property type="entry name" value="Vac8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47249">
        <property type="entry name" value="VACUOLAR PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47249:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0926">Vacuole</keyword>
    <feature type="repeat" description="ARM" evidence="9">
        <location>
            <begin position="132"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="9">
        <location>
            <begin position="174"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="9">
        <location>
            <begin position="215"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="9">
        <location>
            <begin position="256"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="9">
        <location>
            <begin position="299"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="9">
        <location>
            <begin position="340"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="9">
        <location>
            <begin position="381"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="35"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="582"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="582"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="596"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ92483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="642" mass="70003" checksum="920A529AC18AC179" modified="2017-01-18" version="1">MGICCSCMSSNTHRTHEGQAEEYLSEQTGLLSHNASQNAANNNNNHNNNNNNNNIQQGNGQNAALPIAENEREAVASLLQYLEDKDRYDFFSGGPLRALTTLVYSDNINLQRSAALAFAEITEKFVRPVSRDVLEPILILLQNNNDTEVQVATCAALGNLAVVNENKILMVEMGALEPLINQMMCSDVEVQCNAVGCITNLATADENKVKIAKSGALVPLTLLAKSKHIRVQRNVTGALLNMTHSAENRKEIVEAGAIPVLVSLLSSNDPDVQYYCTTALSNIAVDEINRKELAATEPKLVSKLVTLMDSPASRVKCQATLALRNLASDINYQLEIVRAGGLPHLVKLIQSDSIPLVLASVACIRNISIHPMNEGLIVDAGFMEPLVKLLEFNNSEEIQCHAVSTLRNLAASSEKNRLRFFECHAVEKCKKLALTTPISVQTEISACFAILALDESCKLKLLETDIIDVLIPMTYNSNIEVSGNAAAALANLCSRIGTNNYEKIIKAWNSPQDGISGFILRFLTSEYPTFEHIGLWTILQLLESHDSNVAQEIQNNKDMIKSIKHLAEVNYEAAQRANNALSSNNTSINNGSTSNDTHTNHGSSINEAENNGNNGGNNGGEQYDDASLELYSITQKILQFVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R018</accession>
    <name>A0A1E5R018_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein AFG1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g4654</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AFG1 ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000017">
        <property type="protein sequence ID" value="OEJ80248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R018">
        <property type="interactions" value="801"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R018"/>
    <dbReference type="InParanoid" id="A0A1E5R018"/>
    <dbReference type="OrthoDB" id="548867at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005654">
        <property type="entry name" value="ATPase_AFG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040713">
        <property type="entry name" value="ZapE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169:SF6">
        <property type="entry name" value="AFG1-LIKE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169">
        <property type="entry name" value="ATPASE N2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03969">
        <property type="entry name" value="AFG1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="525" mass="59767" checksum="AA3A30981EBEEBA6" modified="2017-01-18" version="1">MMTSLATLKPSLSLLSGCRPVSTHACLLQALKRTATVSVPASAQTXSEAQASELKHVNEAPLERYNRLVKMGKLNDDQYQREIIESLGFLYKDLTNYKPPFVERPDPMSGGSSSFFSRVLHPFQSLQLVNQNEYQYTSKVPKGIYLYGDVGCGKTMLMDLFYECIPTHLSKKRLHFHQFMQLVHKRCHEIIHSVPXIASSGGVSKKTNSFDPIPTLAYELSQESNILCFDEFQVTDVADAMILRRLISQIVSDKYGVIFFATSNREPDALYINGVQRESFIPCIELLKSKNHVIFLNSPTDYRKIPKPISSVYYIPPYGVKYASKECVVKRGAHITQWYNYFAQTDSPHIKAHEQLTVWGRKLDIPHCTAPYVAQFXFKQLCGGNLAAGDYLELAKKFKAFIVTDIPYLSVLRRDEVRRFITFLDAVYDNHGKLATTAAAKFTDLFEEPDHIINDFEIDLKEQRYKPPAEKPETEIDDVLVSEHGFSKEIATKAKIFALDEERFAFARALSRLAQMSTTEWVERT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R046</accession>
    <name>A0A1E5R046_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Flocculation protein FLO1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g4634</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000014">
        <property type="protein sequence ID" value="OEJ80261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R046"/>
    <dbReference type="InParanoid" id="A0A1E5R046"/>
    <dbReference type="OrthoDB" id="3973125at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000128">
        <property type="term" value="P:flocculation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001389">
        <property type="entry name" value="Flocculin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00624">
        <property type="entry name" value="Flocculin"/>
        <property type="match status" value="56"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="3123"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ80261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="3123" mass="342109" checksum="C92769963C19A9D1" modified="2017-01-18" version="1" fragment="single">MSTSTYGTSFSTFAGVDGHETVNTVYYIETPVSRSSSTYYSYWDGTTDSTYSTEVDTITIGGSPVISTVFYVATAEAHGTIERTGSWTGTVTQTYXTSFSTFAGGDGHETVDTYYFVETPITRVTETSYSFWSGSVNSTYSTEVTTNYVFNSSALISTVYYVATPEEQRSFTSTSHWTGLNTATYSTSYSTYTGVDGSATADTVYYVETPVSRYVDTSSNSFWNGTTDSVYSTSVFTAFNANDSSPYISTVYYVATPEAHGAYTKTTYWTGLDSLTYSTSFSTFAGSDGHETVDTYYFVETPISRSXXXSNSFWNGTTDSVYSTSVFTAFNANDSSPYISTVYYVATPEAHGAYTKTTYWTGLDSLTYSTSFSTFAGSDGHETVDTYYFVETPISRSVSTSYSFWNGTTDSVYSTSVFTAFNANDSSPYISTVYYVATPEAHGAYTKTTYWTGLDSLTYSTSFSTFAGSDGHETVDTYYFVETPISRYISTSYSYWDGTSDSTYSTSVFTVTSGGSAAISTVYFVATPEAHATFTQTSYWTGMSTSTYGTSFSTFAGVDGHETVNTVYYIETPVSRSSSTYYSYWDGTTDSTYSTEVDTITIGGSPVISTVFYVATAEAHGTIERTGSWTGTVTQTYSTSFSTFAGGDGHETVDTYYFVETPITRVTETSYSFWSGSVNSTYSTEVTTNYVFNSSALISTVYYVATPEEQRSFTSTSHWTGLNTATYSTSYSTYTGVDGSATADTVYYVETPVSRYVDTSSNSFWNGTTDSVYSTSVFTAFNANDSSPYISTVYYVATPEAHGAYTKTTYWTGLDSLTYSTSFSTFAGSDGHETVDTYYFVETPISRSVSTSYSFWNGTTDSVYSTSVFTAFNANDSSPYISTVYYVATPEAHGAYTKTTYWTGLNTATYDVSYTTYAGSDGHETVDTYYFVETPISRYISTSYSYWDGTTDSTYSTSVFTVTNGSSPFISTVYFVATPEAHATFTQTSYWTGLSTSIYDTSFSTFAGVDGYETVNTVYYIETPVSRSSSTYYSYWDGTTDSTYSTEVDTITIDGSPVISTVFYVATPEAHGTIERTGSWTGTVTQTYSTSFSTFSGDNGRATVDTYYFVETPITRVTETSYSFWSGSVNSTYSTEVTTNYVFNSSALISTVYYVATPEEQRSFTSTSHWTGLNTATYSSSYSTYSGGDGSATVDTVYFVATPMSRYVDTTLNSFWNGTTDSVYSTTVFTAFNANDSSPYISTVYFVATPEAHGAYTKTTYWTGLNTATYDVSYTTYAGSDGHETVDTYYFVETPISRSVSTSYSFWNGTTDSVYSTSVFTATDSSNGSPFISTVYYVATPEAHGAYTKTTYWTGLNTATYDVSYTTYAGSDGHETVDTYYFVETPISRSVSTSYSFWDGTTDSVYSTTVFTGLNANDSSPYISTVFFVATPEQHGAYTKTTYWTGLNTATYDVSYTTYAGSDGHETVDTYYFVETPISRSVSTSYSFWNGTSDSVYSTSIFTVNNGSSPVISTVYFVATPEQHGTYTSTTHWTGLNTATYDVSYSTFSGADGHETVDTYYFVETPMSRYISTSYSFWNGTSDSVYSTSVFTVTNGSSPFISTVYFVATPEAHATFTQTTYWTGLNTSVYSTSYSTFAGVDGHETVNTVYYVETPVSRSSSTQFSYWSEATSSTYSTAVATVTISGSPVISTVYYVVTPEGHGTIERTISWTGLETQTYSTSFSTFSGTDGHETVDTFYFVETPISRVVETSYSYWSGAANSTFSTEVTTNYVFNSSALISTVYYVATPEAHRSFTSVSHWTGLNTATYSSSYSTYSGADGQETVDTVYFVETPMSRYVGTTSNSFWNGSYNSVYSTTVFTGFNANDSSPYISTVFFVATPEQHGAYTKTTYWTGLNTATYDVSYTTYAGSDGHETVDTYYFVETPISRSVSTSYSFWNGTSDSVYSTSIFTVNNGSSPVISTVYFVATPEQHGTYTSTTHWTGLNTATYDVSYSTFSGADGHETVDTYYFVETPMSRYISTSYSFWNGTSDSVYSTSVFTVTNGSSPFISTVYFVATPEAHATFTQTTYWTGLNTSVYSTSYSTFAGVDGHETVNTVYYVETPVSRSSSTQFSYWSEATSSTYSTAVATVTISGSPVISTVYYVVTPEGHGTIERTISWTGLETQTYSTSFSTFSGTDGHETVDTFYFVETPISRVVETSYSYWSGAANSTFSTEVTTNYVFNSSALISTVYYVATPEAHRSFTSVSHWTGLNTATYSSSYSTYSGADGQETVDTVYFVETPMSRYVGTTSNSFWNGSYNSVYSTTVFTGFNANDSSPYISTVFFVATPEQHGAYTKTTYWTGLNTATYDVSYTTYAGSDGHETVDTYYFVETPISRSVSTSYSFWNGTSDSVYSTSIFTVNNGSSPVISTVSFVATPGQHGTYTSTTHWTGLNTATYDVSYSTFSGADGHETVDTYYFVETPISRSVSTSYSFWNGTSDSAYSTRVFTSFNANATSLYISTVYISTSYSYWDGTTDSTYSTSVFTVTNGSSPFISTVYFVATPEAHATFTQTSYWTGLSTSIYDTSFSTFAGVDGYETVNTVYYIETPVSRSSSTYYSYWDGTTDSTYSTEVDTITIDGSPVISTVFYVATPEAHGTIERTGSWTGTVTQTYSTSFSTFSGDNGRATVDTYYFVETPITRVTETSYSFWSGSVNSTYSTEVTTNYVFNSSALISTVYYVATPEEQRSFTSTSHWTGLNTATYSSSYSTYSGGDGSATVDTVYFVATPMSRYVDTTLNSFWNGTTDSVYSTTVFTAFNANDSSPYISTVYFVATPEAHGAYTKTTYWTGLNTATYDVSYTTYAGSDGHETVDTYYFVETPISRSVSTSYSFWNGTTDSVYSTSVFTATDSSNGSPFISTVYYVATPEAHGAYTKTTYWTGLNTATYDVSYTTYAGSDGHETVDTYYFVETPISRYISTSYSYWDGTTDSTYSTSVFTVTNXSSPFIXTXXFVATPEAHATXTQTSYWTGLSTSTYDTEFSTFAGVDGYETVNTVYYIETPVSRSSSTYYSYWDGTTDSTYSTEVDTITIDGSPVISTVFYVATPEAHGTIESTGPWTGPVTQPYSTSFNTSPGDNGRAPVDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0E5</accession>
    <name>A0A1E5R0E5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Pre-mRNA-splicing factor SYF2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g4578</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">May be part of a spliceosome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the SYF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0E5">
        <property type="interactions" value="154"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0E5"/>
    <dbReference type="InParanoid" id="A0A1E5R0E5"/>
    <dbReference type="OrthoDB" id="199717at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013260">
        <property type="entry name" value="mRNA_splic_SYF2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08231">
        <property type="entry name" value="SYF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0747">Spliceosome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="60"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="236" mass="27821" checksum="9B661EEAA1F38DB8" modified="2017-01-18" version="1">MAKLVSMTQPSSPNSSTMQHSKIDPHXQTSKMSDFEXRLKAVRKKRVDLKIKMRKDVQLWEQQGVRRTEKEDSYEENSTDWESNRSRDKKSDFDYTMRDFERWESSKKTQSDDQSKTDLSDYDVLSKHTYDKEIAALVRERNVSTXKVSIAKASSPSVVVQQAEHGLLVNDDKLKVQDLANRLKRITDKRYKKRTRDMIKREEQFSAENNLGDAYVNEKNKQFNMKLDRELAKRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0F1</accession>
    <name>A0A1E5R0F1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative vacuolar membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4581</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0F1">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0F1"/>
    <dbReference type="InParanoid" id="A0A1E5R0F1"/>
    <dbReference type="OrthoDB" id="409725at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052706">
        <property type="entry name" value="Membrane-Transporter-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43310:SF4">
        <property type="entry name" value="AFR304WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43310">
        <property type="entry name" value="SULFATE TRANSPORTER YBAR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="364"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="399"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="472"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="529"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="612"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="665"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="7">
        <location>
            <begin position="735"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1103" mass="124300" checksum="E5D8DF2C17E3D7FA" modified="2017-01-18" version="1">MKTKNRRKWSVNLPINTPSAXDAYSTSHNTNSNNSNQKNDVNFFARSYVGSFHNTFNSNNSMGQENTSLPLYHKNRKSSLSHANGXYQPSNENGIDYEDAXSEEIIVDANGNGXDSAYPRNTPNIHLNGALHEQTVALSNAYDIHQDADGNDLVDEQDNAQMDQQHDSQQNKRLSFDADYSANYEYSSHNNXSITPGRAEQMDEQDLLLLSAISSNTSSVNYATIPGEDTTTSSTDSSTSGISSKNNINVKEIYKYLTNPIFTAKQCVKYMPAVILGLLLNILDALSYGMIIFPITEPIFQHLGPSGLSLFYISSIISQLCYSCGLSDFGCAIGSEMIEITPFFHTMAGMISTYYANDPAHQDAIIATTLVSYCVSSILTGLTFYILGKLKLGKIVGFFPRHILIGCIGGVGYFLCITGIEVSTRIAKFEYNYKFLIDFITQDANWAKLLLPLLLTLALIKAQKIFQNNSLVLPVFYIVTFXLFHFIVIITPTLSMRMLRDNGWVFPLADTKESWFDFYSLFKFNKVNWSLVAQNVPTMLALTFFGILHVPINVPALAMSTGCDKFDVDTELIAHGYSNFISGIFGSIQNYLVYTNSVLFIRAGADSRFAGILLAIMTFVVMVIGPVLISLIPICIVGSLIFLLGYELIKEALFDTWGKLSVFEYLTIMIIVGTMGIFDFVLGIIVGILIACFSFLLDSTKLKTVNGEYDGTVAHSTVNRDYWQTKLLNEIGKQIYVLKLQNLLFFGTIISIEEKIDNMLNNEKIIIKYLILDFKNINADNIDYSAAEGFNRIKRFLDSKKIHLIISSINETDKIYDVFNKVGLLQGIELFNNLNSALEWCENKFLLQYRLLQDKKRKKLSQVHKNSLSKHQLQTQLIRGQQKKPANXLAPAGESFKANSQNLSPFPKTGVRNSISMLSSTPRNNQFFNVAKNIYSTEQDIGTNLQEKYDNNLVYQNFLHSLKMFIEPQDFGKWEKIQSYFRFQKLPPGDTISNDNCFFFVENGLLKISYFVMNNTNRESIYETISNKCCYGKISGDGYAKVAPNYKDEVYCETECSIWIIDENSLKTLRREDPTLFTDLIILIGALNQYRYKKLLAYSLVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0G7</accession>
    <name>A0A1E5R0G7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">54S ribosomal protein MRP49, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g4402</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0G7"/>
    <dbReference type="FunCoup" id="A0A1E5R0G7">
        <property type="interactions" value="120"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0G7"/>
    <dbReference type="InParanoid" id="A0A1E5R0G7"/>
    <dbReference type="OrthoDB" id="1696305at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007741">
        <property type="entry name" value="Ribosomal_mL43/mS25/NADH_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040049">
        <property type="entry name" value="Ribosomal_mS25/mL61"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13274">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13274:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05047">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00916">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein/NADH dehydrogenase" evidence="5">
        <location>
            <begin position="39"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ80386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="137" mass="16190" checksum="350AC085B0E83742" modified="2017-01-18" version="1">MSILQRAQKINKISFTTKLTQVKIDHEKVGKIKLVFQRDNMNGHMGARKFWKNYLPTLKFYNIDLPMEVVRIENKDKNKEIPCILEIQDKSLNKEIAKLDMRNLRDTEIAEKLLELLPHVPVPQNEIIEFVPKIMKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0H3</accession>
    <name>A0A1E5R0H3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Putative protease AXL1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g4535</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0H3">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0H3"/>
    <dbReference type="InParanoid" id="A0A1E5R0H3"/>
    <dbReference type="OrthoDB" id="952271at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000012">
        <property type="entry name" value="Protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050626">
        <property type="entry name" value="Peptidase_M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032632">
        <property type="entry name" value="Peptidase_M16_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690:SF18">
        <property type="entry name" value="INSULIN-DEGRADING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690">
        <property type="entry name" value="NARDILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16187">
        <property type="entry name" value="Peptidase_M16_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 10" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="8">
        <location>
            <begin position="73"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 middle/third" evidence="9">
        <location>
            <begin position="542"/>
            <end position="808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1231" mass="139119" checksum="B96580C9A382B873" modified="2017-01-18" version="1">MXQPAKINFNWQKGRIFQSKRRNPEKEQNRQIPRLLXTSMDSITDYPLSLYHPISGLEKKHKLIKLKNGLLCLLISDPSEKTASCALSVCSGSFNDPSXALGLAHLCEHMILAGGSKKYGHPLAFHEELSRNNGSINAYTSGEQTAFYFNMPNANMLRSXDNYTEIAPYEKLLEQFSSYFEDPLFKNTIISKEINAINNEHYGNVANNGRIMFHATRLLANKSHPFSRFATGNITTLRNLANLEKINLQSELKRYFEEHYFANNNMTLCLKSSQSLNTLAKLAIQNFSDIKSEPTKKLLQKLKKIEKSQRSXKNLDFNILGDVWESKIGXFPLFDNAISSNLICVXSPASSLARLIFPIFHKDYSEYSEKSLKTFYNYWIDILGEESKLSFDNYLKLNEFSTKSSAYQTHFALANDGLVFQMKLTKKGWQNLENILAVFFNQYINEILNTNTERLASTLSESNATDLLKFLYQDSSEASKECSYLCADILTKNFKNAIDPRFLLKSTPSIGSFGDSWNESEKGKSWWNKEAQNFQTFLGRTTTPENVKIVLLGSLKDCALLKDSPWNKEKDLYYEFNYARGDFHISKKAISHDFFSVFGLPSKNSFLPKVAYKTLLVKQALETSRQQSKESFMNLVIGNCSNHSKPQKVAESSEYVYWIKDESHSNIFKSKSMLSIDLTSATLKPSPLNTMFLEILTELISLTIGPQLYAAVKVGYSFSVTPSPKGDVKLKINMAGFTDGLTKILKTVIKVMNGLKEGDFSKEVFRKARVNVRVKYEEASQGNCMQLATIGLYISLEKYVWPFDDRMDALEDDVDIESFQKFMVYFLNSLKLLVIQQGDIGETHESINDFINYLLVTGRNKKLNSSVSKVMTEASTVILKPGDSFIVSQSGAKNDPNNSTTYFLQTGLLNNLYDYTLTALTEFILSLTLVPDLRHKRQIGYVVTGGMRVMSKVCGIHVSAMSSSAPEFLDQSIEDYLMDLGHELGENFTKEKFSSAFVQEYVKILQSSQEMGNFEDSPSGDSDVLSQMQPNYEVGRGHGSLLESQHTFHKKLCNAIASKNHDFKFLSSAQPVDLELLKKLTKDEYMQFFKNKISPKSLKRSKVSIHIISKMNERDKIVKMMSLQLEAFLKIKGFNIPTDKLHEIVEKNGTNTSGLLKDLYRYFSSQGESLKFVLTGLKEVLKALSSLLKVTSSATDGASAPRIKKMKGTSPVACSPCVIVNNPNYFRDTCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0M7</accession>
    <name>A0A1E5R0M7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein phosphatase 2C 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4468</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0M7"/>
    <dbReference type="FunCoup" id="A0A1E5R0M7">
        <property type="interactions" value="310"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0M7"/>
    <dbReference type="InParanoid" id="A0A1E5R0M7"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF837">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="7">
        <location>
            <begin position="61"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="140"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="327"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="150"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="194"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="249"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="507" mass="55963" checksum="23A041F5813467F4" modified="2017-01-18" version="1">MSTIDQEQVAAGTIILDNSTQEMHSDKELNAMNSGSSSDASIPVNSTENYNDSVQYDLSYKVGVAENRNLKFRKTMEDVHTYVENFNNKLDWGYFAIFDGHAGNQASKWCGANLHNLIEQQIQSNEKSEVSDMSKVNKMNGANVKGVGETNESSGESGTTANSAELTGTNVINGISSSEDQTSGDTPESIGTNDEAREPCKDTKEADAKEHKAQDLKQGEIEDLNEQKGSHEKGNNDNNNSHGSDDHHHHNHDHNHHNHHRASLKDIFQQEEDHGGHSHEEQDVRDILNNSFVDADKYICENLPGHSGCTAAVCVIRWEVPVTDTASSRKNSAQSGKSGCASGSANEDTAEEELAHKRMLYTANVGDTRIVLMRDGKAIRLTYDHKASDQYEMKRVEKAGGLIMKSRVNGMLAVTRSMGDKFFDKLVISNPFTTSVELTSKDQFLIIACDGLWDVVEDQEACDLINDNIHDLDPSKAAKFLVDYAMQSGTTDNITVMVIFFSTYEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0Q2</accession>
    <name>A0A1E5R0Q2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cell wall mannoprotein PIR3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4395</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PIR protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0Q2"/>
    <dbReference type="InParanoid" id="A0A1E5R0Q2"/>
    <dbReference type="OrthoDB" id="5415592at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005199">
        <property type="term" value="F:structural constituent of cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054508">
        <property type="entry name" value="PIR1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051153">
        <property type="entry name" value="Yeast_CWMannoprotein_PIR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254:SF1">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22799">
        <property type="entry name" value="PIR1-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184550" evidence="6">
        <location>
            <begin position="20"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Cell wall mannoprotein PIR1-like C-terminal" evidence="7">
        <location>
            <begin position="283"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="367" mass="38555" checksum="5FFAB54F2BB021AD" modified="2017-01-18" version="1">MQFKTLLFSGLLATQVVGAAKHGHSSASSTDVVVTETTTSKSSASSAASSTTQSANATEYVGGEPWSTLTPVETYQCGFTNFNSSFGIAVKTITFNLTSATANASAFATPTGTEAAAATISTSGKSKQNKLFAPTPALDVVIDGQVRAVISKTTTDEVVTTTTLTRTQTVVMSTTTTEGASATGASHNITLIANATAVNETTHGNYTAASNTTTFNVTNMFYNSSNFNTTLLNSTAAFLNSSHFVNSTSYNSTIQCSAQLPSFFNDVSCQTNNTLSVTLQDSVLRDSLDRIGTISSGRQFQFDGPYPQTGAIYAAGWSITPDGFLALGGNDIFYECLSGDEFYNLYDEFIGSQCVPIQLEAVTLIDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R199</accession>
    <name>A0A1E5R199_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative redox protein FMP46, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g4434</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R199"/>
    <dbReference type="FunCoup" id="A0A1E5R199">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R199"/>
    <dbReference type="OrthoDB" id="4044803at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012882">
        <property type="entry name" value="Fmp46"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07955">
        <property type="entry name" value="DUF1687"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ80343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="127" mass="14536" checksum="524F42100A7F6299" modified="2017-01-18" version="1">MFKSLQNAPRVISVFTKTPTNSALLNSITAASNKLSKNYQLEIHTKFPTYDQLQFLNNCKPHSILQSAIPFLKTSSVANFSKDEAKLLSSKELQDIADKKYWFGNKEFWVDWENQKLGDNMTNFPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1A7</accession>
    <name>A0A1E5R1A7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">S-phase entry cyclin-5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4319</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1A7"/>
    <dbReference type="FunCoup" id="A0A1E5R1A7">
        <property type="interactions" value="471"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1A7"/>
    <dbReference type="InParanoid" id="A0A1E5R1A7"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044772">
        <property type="term" value="P:mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000001">
        <property type="entry name" value="G2/mitotic-specific cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048258">
        <property type="entry name" value="Cyclins_cyclin-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00292">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 4" id="KW-0195">Cyclin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="251"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="148"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="408"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="154"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="414"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="535" mass="60479" checksum="9B7A274C38CA5819" modified="2017-01-18" version="1">MQLQGTNYNENTTHNTNATINSVNSAKKRRYSENNCLDGKRVVLGQKNINTNGGNNTDTTDGNNMNKKVCYSSQIDDMTMDTTVISSSPPRVATKSVNNGINNIKTKEHSEPIKTKVYFEPVNEIQTDDMTFVPSSYMDKTMLHATSETNTLPSVSQKKTTVVSSPQQQQQQQQQQSQQEETFQSGSVLSATPQHLQNKDIAACQEYQEEIYAHLYQRETTSHPVINYCAAELKPHCKQYLRASMRAILVDWLVQVHESFRLYPETLYLGVNIMDRFMALKKVSLSKLQLLAITSLFIAAKYEEVVLPKLEKYTYMTDNAYTDQQLKDAEMFILNTLQFDICVTNHFNFLRQYNSLDNYNLNYRLLGKYYMEYATMCHWFIDEKDSVKAMMATVLAKMMYKIKGTSFNTSNAKSSADSSADSSADSSADSSADSSADSSADSSADFSSSQPTSSENSLWGENFVKASHGLDPMHDTALQRKIWLLINEIHKPTTNLKALDNKYNSSSLNYAGPVCKKWCTEIVEMVNDNYSVLFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1S1</accession>
    <name>A0A1E5R1S1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Purine-cytosine permease FCY2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3983</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1S1"/>
    <dbReference type="STRING" id="56408.A0A1E5R1S1"/>
    <dbReference type="InParanoid" id="A0A1E5R1S1"/>
    <dbReference type="OrthoDB" id="2116389at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015856">
        <property type="term" value="P:cytosine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11484">
        <property type="entry name" value="SLC-NCS1sbd_CobB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4160.10:FF:000002">
        <property type="entry name" value="Purine-cytosine permease fcyB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026030">
        <property type="entry name" value="Pur-cyt_permease_Fcy2/21/22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31806">
        <property type="entry name" value="PURINE-CYTOSINE PERMEASE FCY2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31806:SF1">
        <property type="entry name" value="PURINE-CYTOSINE PERMEASE FCY2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002744">
        <property type="entry name" value="Pur-cyt_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="301"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="336"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="433"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="464"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="503"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="542"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="10">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="567" mass="62213" checksum="5CEBCF6E5A4098CB" modified="2017-01-18" version="1">MPVQYTEHEEIINKNELDRFNAVAAANAAVEAEEVVNTNGHQLSNIPDLEKNVNFANVKEDNPLNPQLSTSTQESTTIKQPRPSISSFASSGAYFNRLYEALNVETIGISPIPESHKYDTSLGNIITMWFSANLGVAALSLGTLGPVLFGLDFPTSVWTIVMFNFVGTLPVAYFSVFGSKTGLRQMVLTRFLGGNLTARFFSLLNLVACVGWTAINTGAAAQILSIVNRPHNCPPWAGCLILLGSTVIVTFMGYKIVHLYEKWAWVPCLFVYFVIIARLTISKKFSYGSNTGNYKTTTGDALSFGATIFSFAAGWAPYAADFTVYMPQKVNSWKIFFGVAFGLFFSLTFTLILGSACAISLQNSPEWQTLYDSHGLGGLMYGIMVKDSLHGFGQFCCVVLAMTTVATNTPNMYSISLCAQALWEPLAKIPRPICTLIGSGITLIISICAYYKFLTFMSNMMDAIGYFIGIYLGITLTDFVVFHRCKFTSIQVENWKSWDNLPIGIAGLCAVFAGAVGVAMGMDESYWVGQIARHVGEFGGDIGWELGFCFSALTYALLRPVEKRFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R297</accession>
    <name>A0A1E5R297_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Peroxin-10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">AWRI3579_g4121</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the pex2/pex10/pex12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R297"/>
    <dbReference type="FunCoup" id="A0A1E5R297">
        <property type="interactions" value="284"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R297"/>
    <dbReference type="InParanoid" id="A0A1E5R297"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016562">
        <property type="term" value="P:protein import into peroxisome matrix, receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16527">
        <property type="entry name" value="RING-HC_PEX10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025654">
        <property type="entry name" value="PEX2/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006845">
        <property type="entry name" value="Pex_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23350">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23350:SF0">
        <property type="entry name" value="PEROXISOME BIOGENESIS FACTOR 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04757">
        <property type="entry name" value="Pex2_Pex12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="7" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="20">
        <location>
            <begin position="247"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00041230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OEJ81008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="297" mass="34652" checksum="2E9C7563BA0C0FB1" modified="2017-01-18" version="1">MSNYKLPFADVPAIVVADNKDDEVISHLHNKLKDLSAMLGNKSCLNRMFRLLHWNKDVSLQMVSKFLYLMVTVFVGIRTPGQQFADISYIGNTEFHKNLKLRMKRLLYIFGCSGATAVIQKILVKHFAIHESVVETFCQLHLATFYMNAKYRFFWNRLFGFRYVLQHTFANEEEEEYRKTYSYNYKTIGKLILCRLAYKTLYPCISQLFSKTRSRELQKQKTSHTEQQINLSDPLQLTYIPETSRTCILCSSEMLSPSCGPCGHIFCWTCLIEWCKTKKNCPLCRQACLPQEVVPLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2B3</accession>
    <name>A0A1E5R2B3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DDK kinase regulatory subunit DBF4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4259</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2B3"/>
    <dbReference type="FunCoup" id="A0A1E5R2B3">
        <property type="interactions" value="190"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2B3"/>
    <dbReference type="InParanoid" id="A0A1E5R2B3"/>
    <dbReference type="OrthoDB" id="3973118at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031431">
        <property type="term" value="C:Dbf4-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010571">
        <property type="term" value="P:positive regulation of nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901987">
        <property type="term" value="P:regulation of cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3410:FF:000001">
        <property type="entry name" value="Protein DBF4 homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3410">
        <property type="entry name" value="DBF zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055116">
        <property type="entry name" value="DBF4_BRCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013939">
        <property type="entry name" value="Regulatory_Dfp1/Him1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051590">
        <property type="entry name" value="Replication_Regulatory_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006572">
        <property type="entry name" value="Znf_DBF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038545">
        <property type="entry name" value="Znf_DBF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15375">
        <property type="entry name" value="ACTIVATOR OF S-PHASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15375:SF26">
        <property type="entry name" value="PROTEIN CHIFFON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22437">
        <property type="entry name" value="DBF4_BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08630">
        <property type="entry name" value="Dfp1_Him1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07535">
        <property type="entry name" value="zf-DBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00586">
        <property type="entry name" value="ZnF_DBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51265">
        <property type="entry name" value="ZF_DBF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DBF4-type" evidence="6">
        <location>
            <begin position="853"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="273"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="527"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="654"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="662"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="904" mass="99972" checksum="E7364892F4CF7555" modified="2017-01-18" version="1">MNNFASTGSTRRIPLGETNANQLSLSQTGSLSQKANLAASTVTKKHNYQFFKDNWEHTKAKPVADPQSNSRDSEHGLANMQEDTEVDVTDAAAENGDGPLHDAPEALQQNQNFQKGDEHDASLLACGVVGQDKNSLVQITPKDSTSYRKRLSESLDRQTHNNACALEEGARRKLTTAEFLRKNSGQIAVDAIDAEYQEEKKTSVLDDPMYLSKNKHIATPITKSRRSIGGASLVNPMYTKILPIQSTVQNIGGKPTLTTVSDKEQTNVEMGKVTNKNTTSGNTTTSRSVGATGTTGVSGTAKNSAAMNLKVWQTNWKNIMKKETVIYFDIQEGVATGGKTGAQEMKTLREKDSTKALIALETLRLGYYSLGAAVSKFFDREVTIVITDRSNDASFIKSLSESDVLKKASKNDMKIWTLEKAERFLTHLDVDISKMKKKALNSAALGNASKNGLVSKFGNAIVAAQTTTETGTLRRYLQNEKLYGPMDRDPLAKRDDMHYFSKDRPHIYIYDLNQQFSPLVIMEWKTNKSNSEPGNDLSKDGAGTKNDADLPYPTLKNSTFGRCPFIADNEDETSDKRIFKRLKKDMNQQDQAEFLLRLYSTTAVPNPMNDIYLLNHDKYNDSGKNFYKKFGYHFDELPFAKNNKNSEKSIGKVKIHEPKTPANKNAQQDNQKSNSPKNNSDMGEDGESPKLPQKVVWKRPPLKNKVNIENLLRMETTGLDDSNGFNGVGKNDTATGASFAGKKRPMLSEIKASGVLNNDPINNSSTQPFQANNGLAATKSTVVGKQMNALNSKFVVMDPVPANCNVPKTPSTHVSVPLKPKERIAGSKISLGNKSPVTRKPNTIAINAAIRDKRMGGGYCENCRVKYESLRLHIKTEKHLEFAQKDQNFYAIDSLIENLHSSMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2K1</accession>
    <name>A0A1E5R2K1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ATP-dependent helicase HRQ1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3955</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2K1">
        <property type="interactions" value="105"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R2K1"/>
    <dbReference type="OrthoDB" id="18781at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17923">
        <property type="entry name" value="DEXHc_Hrq1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18797">
        <property type="entry name" value="SF2_C_Hrq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055227">
        <property type="entry name" value="HRQ1_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018973">
        <property type="entry name" value="MZB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47957">
        <property type="entry name" value="ATP-DEPENDENT HELICASE HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47957:SF3">
        <property type="entry name" value="ATP-DEPENDENT HELICASE HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09369">
        <property type="entry name" value="MZB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22982">
        <property type="entry name" value="WHD_HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="3">
        <location>
            <begin position="291"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="4">
        <location>
            <begin position="528"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ80793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1079" mass="122928" checksum="64C8914FBCBE448E" modified="2017-01-18" version="1">MQEIPDGKDGVHTDENKQLASLKKTFKHVNTFYAFLKFKHPYINSLSFRFLKFNLIKQLKREITVEEIQQIVYILNYVPNSTSTATKTINNANEKLIKKVAMFQYISKDQFELENMSMAGSATNQSVNGDIFKLKDDTGEEIDISEILVLRLKQNKATTMDVLKQYIKHRETIFNKNLQTLLESHGLPQLIEILKNVLLPKPTVHVDPIEQMMKKSKXRKRKFEGDAEEGAEYDENKDRIESFIHDYLHPKIVHEVPKSDPQYDSIEFLPSIIQELLHPIDKLYTHQYQAISSILHGENVMVTTSTSSGKSLIYQISTLLQLIQSTKACIIYISPTKALAQDQKRSFQNFLSKLHQLPNIHQDPIFNQYLKNLEVATYDGDTPVEERRRIRLNHNIRVIFTNPDMLHTSILPNHLLWKRVLSHLKIVILDELHIYNGLFGTHVSFVMRRMNRLLKYYAANNKFIGCSATLKNPYNAFVELTGCSDVTIIDKDGSPRGLKNVVVWNPDPVTTTSSMKYKRDFIGESANIIVQLMRXDIKTIAFCFVRRLCELLMKEVRKILQNNDERGDVELLQDVVAYRGGYSPEDRRKIEKELFHGNLKCCISTNALELGVDIGELDCVLMCGFPVSLGNFYQQSGRAGRRMNDSMTIVVASDSPVDQHYATHSEQLVRIDPKNLQDLVIDLNNSLVLTSHLQCAAFEFPIDLEKDHEFFPCDNFRSIVENKLSFNGTSNKDKNLNGTYTCDIRFLPWPAQNISLRGAQEDMFAVVDITNGRNMVIEEIEASRTSFTLYDGSIFIHQGLSYIVKEFNADIHYAKVLRTDVEYTTSQRDFTDVDPVEIEMIRSLGKESDVPVYFGKIKTTIKVFGYFKIDKFGKVIDAVETYNPAIHLFSKGLWIDIPKRAMDIVEQKNLCMSAGVHAAQHCLISCLPQYIVAGVDEVQTECKAPEKEFAGRQTARVRPARLVFYDSRGGSYGSGLSIKAFEHIDEVLNQCLEKLQECDCKIGCPNCGAAVPFCKESNLVISKSACMVIIAVLLGKDTEEFLDGVEDGPEENLPEIKVETVKPIDDHVKFAPDFKIVNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2M9</accession>
    <name>A0A1E5R2M9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PHO85 cyclin-5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4025</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2M9"/>
    <dbReference type="InParanoid" id="A0A1E5R2M9"/>
    <dbReference type="OrthoDB" id="286814at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20557">
        <property type="entry name" value="CYCLIN_ScPCL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF36">
        <property type="entry name" value="PHO85 CYCLIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin N-terminal" evidence="2">
        <location>
            <begin position="157"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ81140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="281" mass="31600" checksum="F17BFB26AF05CD5F" modified="2017-01-18" version="1">MQTNSIENTKTHGTSVKSTTFESKTGMIPSPAHDLSGIDNTRNEPITTTLPSLKHKPKSYRSTTAHPTPVHTINTKSIDISKSNSQIDVECGFQQSAKIDHNVDEPASKPVSLLNKNDXAHVAATPASSSNATNIMILKISKVLLKTDLPHKRRAFQHYATFLHDVLKRSKTTKTCLILATYYYDLLINETKTPKQTSNEVESLEYCAKRRFYGCLILAHKYINDKTYNMESWSIISGKGGLSKLDISKIEKWCLAKLNYELYVKDLSVLQKWCLDKLLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4W0</accession>
    <name>A0A1E5R4W0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NADPH dehydrogenase 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3494</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4W0">
        <property type="interactions" value="869"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4W0"/>
    <dbReference type="InParanoid" id="A0A1E5R4W0"/>
    <dbReference type="OrthoDB" id="3969599at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02933">
        <property type="entry name" value="OYE_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000138">
        <property type="entry name" value="NADPH dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045247">
        <property type="entry name" value="Oye-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893">
        <property type="entry name" value="NADH OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893:SF91">
        <property type="entry name" value="NADPH DEHYDROGENASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="4">
        <location>
            <begin position="18"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ81955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="397" mass="44774" checksum="63F745E21B6E4249" modified="2017-01-18" version="1">MSYTLDPKYKPVTMKDSNLFKPIQVGEMHLKQRIAMAPLTRGRATDDNVPNKDGMAAKYYGQRSQAPGTLIITESSYISEQAGGYANVPGLYNMEQMEAWKHVFHKIHENESYVFVQLWSMGNQADPVFLKSXSMKFLGPVDGLYPNEQSKNKAIECGNELKGMNQAEINQCVADYVQAAKNAVMHGADGVEIQAGNGYLLNQFLDPISNKRNDKYGNSIENRSRFVLEVVDALTAAVGASKIGIRFSPFASHNEMSGRAEPLIVAQYSHIIGELEKRAFANPERRLAYIHLREPRVPLPNQKEIHHSDPDFSNDFVFTIWKGIVIRSDNYALDNHAALQDVNANDRTLLAYGRYFISNPDLVYRLKHELPLCYYDRSTFYKGNSSVGYTDYSNYSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4W2</accession>
    <name>A0A1E5R4W2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Vacuolar protein sorting-associated protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3515</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4W2"/>
    <dbReference type="FunCoup" id="A0A1E5R4W2">
        <property type="interactions" value="1008"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4W2"/>
    <dbReference type="InParanoid" id="A0A1E5R4W2"/>
    <dbReference type="OrthoDB" id="29072at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02678">
        <property type="entry name" value="MIT_VPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19521">
        <property type="entry name" value="RecA-like_VPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000043">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000015">
        <property type="entry name" value="vacuolar protein sorting-associated protein 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.80:FF:000014">
        <property type="entry name" value="Vacuolar protein-sorting-associated protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045253">
        <property type="entry name" value="VPS4_MIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF83">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04212">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00745">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIT" evidence="11">
        <location>
            <begin position="6"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="10">
        <location>
            <begin position="165"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="84"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="364"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ81911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="439" mass="48893" checksum="C7EB9D5799CD409F" modified="2017-01-18" version="1">MSASGSGDFLTKGIELVQEAIELDTGKKYEEAYKAYYNGLDYLMLALKYEKNPKSKDLIRAKFTEYLNRAEQLKDHLEKLQEQEQQNKNGGAKKKQGTNNTNNNSNEDDDSDKKLRGALSGAILTEKPNVQWDDVAGLDGAKEALKEAVILPVKFPQLFTGKRKPLSGILLYGPPGTGKSYLAKAVATEANSTFFSISSSDLVSKWMGESERLVKNLFNMARENKPSIIFIDEVDALTGQRGEGESEASRRIKTELLVQMNGVGNDSSGVLVLGATNIPWQLDSAIRRRFEKRIYIPLPEAQSRTKMFQLNVGDTPCSLTKEDYRTLGKLTEGYSGSDISVVVKDALMEPIRKIQMATHFKNVTPKDDPDAPRKFTPCSPGDPDAIEMSWTEIEGDELQEPELTIKDFIKAVKNSRPTVNQQDLTKQLEFTKDFGQEGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R552</accession>
    <name>A0A1E5R552_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADP-ribose 1''-phosphate phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.84</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3781</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>ADP-alpha-D-ribose 1''-phosphate + H2O = ADP-D-ribose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:25029"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="ChEBI" id="CHEBI:58753"/>
            <dbReference type="EC" id="3.1.3.84"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the POA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.84" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R552"/>
    <dbReference type="FunCoup" id="A0A1E5R552">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R552"/>
    <dbReference type="InParanoid" id="A0A1E5R552"/>
    <dbReference type="OrthoDB" id="2155246at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140291">
        <property type="term" value="P:peptidyl-glutamate ADP-deribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050892">
        <property type="entry name" value="ADP-ribose_metab_enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12521:SF0">
        <property type="entry name" value="ADP-RIBOSE GLYCOHYDROLASE OARD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12521">
        <property type="entry name" value="PROTEIN C6ORF130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="5">
        <location>
            <begin position="1"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ82024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="211" mass="23687" checksum="4AF5D06335185B1D" modified="2017-01-18" version="1">MSNITYVKGNILSPTISKKRIIIHSCNCLGTWGGGVAYQLGQKYPKTELEYTKICDYFSNDDPRKLLGNFVILPSFKDSKTLIGCLFTSLGGGGSHDSASSILENTQLSLQKLLKYIQQGKKYCAPPNSPSKQVSINTDVFETKAEEEEIGLKLLLELMNEQYFSQFNEIQDLKHFELEMPKINSGIFGVPWENTEKILEALPCDITVYDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5B8</accession>
    <name>A0A1E5R5B8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">triacylglycerol lipase</fullName>
            <ecNumber evidence="1">3.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3546</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.3" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5B8">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5B8"/>
    <dbReference type="InParanoid" id="A0A1E5R5B8"/>
    <dbReference type="OrthoDB" id="406844at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051299">
        <property type="entry name" value="AB_hydrolase_lip/est"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46640:SF3">
        <property type="entry name" value="LIPASE LIH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46640">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G06510)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fungal lipase-type" evidence="3">
        <location>
            <begin position="106"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ82082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="352" mass="41015" checksum="06D5BC8CAD13FB61" modified="2017-01-18" version="1">MXPIAKTFELQHLKENTEIRVTQDTYDKFVYFXKMCALATCITDYKLKVGETLYDGGCPSNLKFCSDLNVNPALKDTXIEVLLKLEEYQLGTGFLAVDHFHKILILVFRGSTTSWDWFHDFLLMPSAYDPYSKEHYEKQVGEGKVPPCENCKLHYGFXNFWKTLHPEFVNRLESFAMKYPDYKLXITGHSLGGVLATMAGVEFKLRGFEPQVVTFAQPKLFNSEMAHWVNQLFXVYKXSQKIEDTREVEMFCENKGGLYRVVHENDQLVDIPFFFHHAGLQIDIKKYETPHEIQDLKYVGVSKDVGTSSFNSSTNTLEKEAMSTEMELYQEDWLHMYEHRNYFIRINECGYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5F9</accession>
    <name>A0A1E5R5F9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Bypass of stop codon protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3821</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R5F9"/>
    <dbReference type="STRING" id="56408.A0A1E5R5F9"/>
    <dbReference type="InParanoid" id="A0A1E5R5F9"/>
    <dbReference type="OrthoDB" id="413079at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051788">
        <property type="entry name" value="MFS_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23514">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23514:SF3">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="183"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="271"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="344"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="382"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="415"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="439"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="479"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="507"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="118"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ82145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="540" mass="59854" checksum="7E8F86B6CE2C7664" modified="2017-01-18" version="1">MSHDDRPHNDLIEAHSQAPTLVGTPVSEKSLSFMDDSDARVLVPVDSTQKVNAFEHTREVYAEDDRVGSPVPLDVDIIDVPYSSNECYTTDYYLDGETPRQVVLYPLDYEKCKLIYLQVVSCVVMFTIFGMNDQTTGSLMPLLTKHYQVSELSVSYIFIMQVLGYTISALVNGKVHKMVGRRGVFLGSGALFIVFIGILSLKPYFPIYCFCFFPIGLGIGLMDSTANVLFGNFVKSKNEIMGIVHGTYGVASIVTPLVATHYDETNWNKIFYLPLAASIIGTLMSFVAFRHESGAKYNYLVEKSSLQNLQTDLGETGVGSTTTEDDDMFNGNDSISNLIRQPIIILYASYVFFYQGTQAAVGTWLFTYLLKVKDGAPKQMSWIISGFWGGLTLGRFALGPITSRCFKNEYTANKAYGVVSILSFCGMFLLSFFPFTYKFFAIIFAIFTGGAGFGLAALFPNVNVVMMEILPEKYQVESVGISISLGISGTAVIPWVCGLFVHHLGYSILPFLYVVSILGLQGIWLLYPKMIKTSKAKEVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7C9</accession>
    <name>A0A1E5R7C9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Iron-sulfur cluster assembly protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3394</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8 9">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; iron-sulfur cluster biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the NifU family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7C9"/>
    <dbReference type="FunCoup" id="A0A1E5R7C9">
        <property type="interactions" value="550"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7C9"/>
    <dbReference type="InParanoid" id="A0A1E5R7C9"/>
    <dbReference type="OrthoDB" id="1925777at2759"/>
    <dbReference type="UniPathway" id="UPA00266"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06664">
        <property type="entry name" value="IscU_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1010.10:FF:000005">
        <property type="entry name" value="Iron-sulfur cluster assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011339">
        <property type="entry name" value="ISCU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002871">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01999">
        <property type="entry name" value="iscU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10093">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY ENZYME NIFU HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01592">
        <property type="entry name" value="NifU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82649">
        <property type="entry name" value="SufE/NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="7 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="NIF system FeS cluster assembly NifU N-terminal" evidence="10">
        <location>
            <begin position="54"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ82807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="192" mass="20918" checksum="7309EDEE70C4E74B" modified="2017-01-18" version="1">MFRQMKQMKQVMYASTLSKTVFNQGVIGNRVGGYTFNGINRTSGSTLNRFSRSYHAKVIDHYTNPRNVGSLDKKLNSVGTGLVGAPACGDVMKLQIQVDEKTGVIQDVKFKTFGCGSAIASSSYVTELVRGKTLEDAVKIKNTEIAKELSLPPVKLHCSMLAEDAIKAAIKDYKSKRVGTNISNGTTHKHGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7N7</accession>
    <name>A0A1E5R7N7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Calcium permeable stress-gated cation channel 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3161</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7N7"/>
    <dbReference type="FunCoup" id="A0A1E5R7N7">
        <property type="interactions" value="96"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R7N7"/>
    <dbReference type="OrthoDB" id="1689567at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF5">
        <property type="entry name" value="RE44586P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="412"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="465"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="505"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="549"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="596"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="623"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="660"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="9">
        <location>
            <begin position="22"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="10">
        <location>
            <begin position="217"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="8">
        <location>
            <begin position="410"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ82929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="790" mass="90276" checksum="032B53484C1CEDBB" modified="2017-01-18" version="1">MNSSTVFKTDPPDIRKPPAKVVSTQFYTALLLGLFALLSFSILLKKLPKLYASRKYKIDGIPSWKETSLFGWMPVLFRINDQQVLEYAGLDAFVFLGFFKMCIKFLAICSIFAICIISPIRYHKTGKYDDGDDDGYNPDTYKRYARFVKRSIVSILDNTEPSDPSLPPDATKEKYLWMYVIFTYIFTSLALYMLVKQTKLVVNIRQKYLGRQNKITDRTIRITGVPVEQRNEESLINTIETLKIGKVSSITICREWGYLNRLFKYRKVILKQLESKLADCNPELRDVEHYSESYTTESTSSLATSSKPLMGRPTLRIGGFGFFGKEVDAISYLQEQLKVVDKEIFEARERQYSTTPTAFVTMDSVANAQMAAQAVLDPRVHYFQTHLAPAPHDIIWDNVSLNRHERMMKSNFVTVLIGVISVFLIVPVSYLATLLNLKTITKFWPSLGKFLKENSWAESLVTGLLPTYLFTLLNVLIPYFYEYLTSRQGLVSHSEEEISLVSKNFFYSFVNLFLVFTLAGTASNFWGYFTDTTKIAYQLATSVREFSLFYVDLIILQGIGMFPFKLLLVGSLIGFPFVKSKCKTPRQRQYLYQSPIFNFGLQLPQSILILVITIIYSIMSTKILVSGLAYFVIGFYVYKYQLVFAVDHLPHSTGKVWPLVYRRVILGLLLFQLTMAGTLAGYPSGWILSTCLFPLPIITVMCWWDLQTNYLPLINFIALSSIREFERGSMSQQRPASNILNPANNHSSTFSHVAEDESYEYPFLICPLEPPMISETTNSNNSSDDVLVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7Q9</accession>
    <name>A0A1E5R7Q9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Peroxisomal targeting signal receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3225</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxisomal targeting signal receptor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7Q9"/>
    <dbReference type="FunCoup" id="A0A1E5R7Q9">
        <property type="interactions" value="87"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7Q9"/>
    <dbReference type="InParanoid" id="A0A1E5R7Q9"/>
    <dbReference type="OrthoDB" id="10006023at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005052">
        <property type="term" value="F:peroxisome matrix targeting signal-1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024111">
        <property type="entry name" value="PEX5/PEX5L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10130:SF0">
        <property type="entry name" value="GH08708P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10130">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 1 RECEPTOR PEX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 8" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="437"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="471"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="72"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="73"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="88"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ82911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="602" mass="67950" checksum="CC8E309F0FA5BD3C" modified="2017-01-18" version="1">MADCSVNANPLAKLNKHAGENKLFHQASGRGTSQQSAFQNAFQNAQLNGSQSSLGFTEHNNVMRNKPMLLHHPSQQSLQGSPSPISQHHQQNQWRSEFHSQSAPSLVDLQNQHTQMTNELSSHGHSSSQMFNPNMNMLNTSMRASSSMMLRGNMGASNLAYSARAAAPVSANTSASLQHNQDMSKQWEDQFLELEKQVEQNLTLQEGKQDVQLEKEDDAGIQSFEEVKFEEVWDDLQKQKEELDSSIANENTSTSQGQENDEYYQAYYNMYTNKPTGNYKFTSTETNQYHKNPNAYEMGVILMDQGAKLSEAILCFEAAVQQDEKHLDAWLKLGQCNIANELETQGIAALEKVIELNPQHLEAMMNLSVSYINEGYDLTAYNMLSNWLQIKYQDSSSNYKPVTQDKHGHELSILDKTLNKFNHILSNNGRSIALQDKEFQLGLGLLYYTKGDFDQTIDCFQTALEAQPEDEIMWNRLGATLANANRSEDAIHAYQRALQLKPTFVRARYNMAVACINIGCFKEATESLLGALSLHEVEDSNNAGLFETKNATDSLLYEKYSANIMETLKRAFIAMNRRDLLERIGPTGTKLELSQFRSEFDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7U1</accession>
    <name>A0A1E5R7U1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7U1"/>
    <dbReference type="InParanoid" id="A0A1E5R7U1"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ82964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="269" mass="29877" checksum="4C3FAB1FDC26A7DA" modified="2017-01-18" version="1">MASKILSSRPVVSLPFNALNNSKRRRVAVGHYTNTFNNAKIIATIYRHYLNNSINHDNNGNTSSNADNSVSHTDSIDKNVMKNNIGTALVISLVRNIESVYSTQGLSNGVIKGTTSVHNPQKAGISWHNSNVFLPTHTKLVKFLKGCVLRFKVSYTDLDKISLNILKFLKEHSKVFVEKLHSNIYKLVLGLVFTVLGSRIDKKLIGVNNPFLPGGVEELQTIANIITSASSSFWFFTTKLSTANANTSSFSIVDKKVVDRNMNHRHYVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7V3</accession>
    <name>A0A1E5R7V3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine/threonine-protein phosphatase 2A activator</fullName>
            <ecNumber evidence="6">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Phosphotyrosyl phosphatase activator</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3419</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PTPA-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7V3">
        <property type="interactions" value="537"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7V3"/>
    <dbReference type="InParanoid" id="A0A1E5R7V3"/>
    <dbReference type="OrthoDB" id="16120at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008160">
        <property type="term" value="F:protein tyrosine phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04087">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1150:FF:000002">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004327">
        <property type="entry name" value="Phstyr_phstse_ac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043170">
        <property type="entry name" value="PTPA_C_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037218">
        <property type="entry name" value="PTPA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012:SF5">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A ACTIVATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03095">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016325">
        <property type="entry name" value="Phstyr_phstse_ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140984">
        <property type="entry name" value="PTPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0697">Rotamase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ82995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="369" mass="42520" checksum="E4B7DD7A0A35D51A" modified="2017-01-18" version="1">MTLLEKRLLTVQDMELWKESSTYHELLEFIEKLAHSVAGCENNHYALPISANVEKTCXILDEIVSIFKLHPVVKDKETTRFGKLEFRDFYDEVSNRNKQLISEXFETLQKEQVEELSVYLLNSFGDRQRIDYGSGHELNFLCFLFGLYKLNVLTENDYTNTVLKIFINYLHLMRLLEKEYWLEPAGSHGVWGLDDYHFLPFLFGAFQLSTHKHLKPKSIHNEEVVEMFASKYLYFGCIAFINNVKTTASLRWHSPMLDDISGVKXWAKVSEGMIKMYKAEVLGKLPIMQHFFFGSFLKCPDGVSPPGTHKDHDHSGCKHSELQNTWGDCCGIKVPSALAANEANKIKSEKHGNLNHSKVKAQVKRLPFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R816</accession>
    <name>A0A1E5R816_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Ribosome biogenesis protein NOP53</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3405</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">May play a role in ribosome biogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the NOP53 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R816"/>
    <dbReference type="FunCoup" id="A0A1E5R816">
        <property type="interactions" value="678"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R816"/>
    <dbReference type="InParanoid" id="A0A1E5R816"/>
    <dbReference type="OrthoDB" id="5072at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011687">
        <property type="entry name" value="Nop53/GLTSCR2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14211">
        <property type="entry name" value="GLIOMA SUPPRESSOR CANDIDATE REGION GENE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14211:SF7">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN NOP53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07767">
        <property type="entry name" value="Nop53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017302">
        <property type="entry name" value="Gltscr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="90"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="269"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="432"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="104"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="291"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="312"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="432"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="442"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR017302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ83052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="453" mass="51828" checksum="EAA65BA3743AB034" modified="2017-01-18" version="1">MSAPSQFKQSSRKGKKAWRKNIDISDVEKGLQKAKDLEITHGTTDINDLADANLFQIDDKGDENLQNKLVKRNQIKKNLKSKEILDSIATNSKVQPLGRHPNNHKHENGKTSDKKIQGVSSKKLKKLLELSGKSLSTKDRAAAHLENHGIVRKSKSKDLWGQEDGLVSANPTYLSKITKEQKAELSLPEELFTQSSSSWSTCTVQPKSMKIAPLTVRELESLPNAGKSYNPDTESWQKLIDTEYSLEQKRELRRLEIEQYRAKIQHLMETLDDNEEEDSSDEEGDDDGEDNETKESGDDQVKRLSINPVVQNKKKTKYQRNKQKRHQEKVNLQKEIKALKERVKALENLNEVEQSIESKDPAHITAVGKDISKKVQKRQKLGSKHNVMEDALEVKFQDELTGSLRQLRPEGNLLYDNVRKLQSSGKIEARIPTRKGRKHKPRVTEKWTYKDFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R875</accession>
    <name>A0A1E5R875_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Chromatin assembly factor 1 subunit p50</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3296</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R875">
        <property type="interactions" value="89"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R875"/>
    <dbReference type="InParanoid" id="A0A1E5R875"/>
    <dbReference type="OrthoDB" id="427795at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050459">
        <property type="entry name" value="WD_repeat_RBAP46/RBAP48/MSI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22850">
        <property type="entry name" value="WD40 REPEAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="5">
        <location>
            <begin position="44"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="137"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="137"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ83094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="552" mass="61135" checksum="D2B9E5A6C1D384D3" modified="2017-01-18" version="1">MTYTNPMDTPQTPMNKIHMPVLQDKELHAQPDYGYDINEKNQTNYQTWKKNARHLYDYLNTNTCRWPSLSVQIFPDIHEPTLSRRLLLSQFTSGQIPQDEMVYLSSISTSIPWSSINNFEMDYMEFHVDSAPTVTTTATTTTTTTTTTAAAPNAPNAPNAPNASNAPNALSAPTNTTTTTTXSSNPAKSQKRKKNLDREIEIQFPVGEECNRTRYMFSNPDVIGSVSSKGSVYIHNKTKCLSNTVTTTPEFRLECGPMFDSGGDVSYFDPNFESLSLSWNFQQEGVLSTCANDGSVFVWDLNKVYCKRDLSCLTGPSSQDTCPSSSTYENRIKIDPVGCNDVSWMVNHRNIVAAACETQGLQMIDTRNNTTRSSCSSNRSGISLNSVQFNWQNDMLLCTGSSTGDVSIWDLRSLSCPLQNFIHDYKTGALANVQWNPLLPNIVASAGLEDGLVKIWDTSAAGTTTPTLNSTTYAPGEGTATEKMHTETTNTTDTTQNNSNNGNLIFTHGGHMLGCNDVCWDFHDEWLLASASNDNSIHLWKPAKRAIAEYYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R891</accession>
    <name>A0A1E5R891_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcription factor TBF1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3377</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R891"/>
    <dbReference type="InParanoid" id="A0A1E5R891"/>
    <dbReference type="OrthoDB" id="3366990at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003691">
        <property type="term" value="F:double-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010833">
        <property type="term" value="P:telomere maintenance via telomere lengthening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11660">
        <property type="entry name" value="SANT_TRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013867">
        <property type="entry name" value="Telomere_rpt-bd_fac_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052833">
        <property type="entry name" value="Telomeric_DNA-bd_trans-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47807">
        <property type="entry name" value="PROTEIN TBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47807:SF1">
        <property type="entry name" value="PROTEIN TBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08558">
        <property type="entry name" value="TRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="5">
        <location>
            <begin position="621"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="58"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="536"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="585"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="63"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="113"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="536"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="554"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="585"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="6">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ83126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="786" mass="89740" checksum="1A19DB1B7E157C49" modified="2017-01-18" version="1">MTTDAADIKKLYFSNNEELFESLSSQATVNLQIHHSLREIATKIVFFLIGAVFIDNDNDDNDEHNVSKDANGKNTDEIKEDKHDTTNNAGKKDDDDDDDDDDDDDDDGDDGDDSKQLEDARTSESNKNESIVNKSIHHTAQLQFQNANVILELLYGEQSTSSNKDXNTKQFFLLVELFKKLVLDFLHKPDYTINIDESXGSWESTTTXNDDSKSTNRRLKYFLSFDDFFPGILNNIRHNELVLLTNDLSYKDFLHYMIYNESPNPQPISKRTSLSKDELDVQNIEDFLETSLISLPLQYELLISIQRINLCIYLLSVFGFFPSSMSFLDDFFLEVLGGNSEVNSVEKTENNSFYTSSLEDHSMNDYFPSFFDSDVASSKGTTDFPSIKAITQSGFTPIYRAQLNRLSRQHVILYLELKTQEYISFIQYALEKTGDAHNAVMSTELIETTLEEIFDQRRMKTYLQNRKKSHPNEQEEITIIEQDFLNRCFNRKQHLQKFTSLQKLVQTYTWSDFLLNFVKFAQKRLRFFDNRLKQNSKLQQASSMPTTPMDLYSMPSTPGSTVSAAPSGKSTPSVHLAESHLSEETTITGESITTETAHPMETGNXYSTQESSKPSRKPLVAKPKRKTAWTSDEEDALLTALKVCGPKWTIILDLFGAGGQLSESLKNRAPVQLKDKARNWKMFFLKTEIPVPPYLDKVTGGIERSTTGGNKGKVLKPKRKLIKKTTIRMDLNKFKYNSADLKLNEKLELDYXISSFIQENKVDTEEYNTILDLHNPLLDNSIASPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R8B3</accession>
    <name>A0A1E5R8B3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 16">Diacylglycerol O-acyltransferase</fullName>
            <ecNumber evidence="5 16">2.3.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g3420</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>an acyl-CoA + a 1,2-diacyl-sn-glycerol = a triacyl-sn-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:10868"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:64615"/>
            <dbReference type="EC" id="2.3.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 16">Glycerolipid metabolism; triacylglycerol biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Endoplasmic reticulum membrane</location>
            <topology evidence="1 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the diacylglycerol acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.20" evidence="5 16"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R8B3">
        <property type="interactions" value="223"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R8B3"/>
    <dbReference type="InParanoid" id="A0A1E5R8B3"/>
    <dbReference type="OrthoDB" id="264532at2759"/>
    <dbReference type="UniPathway" id="UPA00282"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004144">
        <property type="term" value="F:diacylglycerol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07987">
        <property type="entry name" value="LPLAT_MGAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007130">
        <property type="entry name" value="DAGAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317:SF0">
        <property type="entry name" value="ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03982">
        <property type="entry name" value="DAGAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 16 18" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10 16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="6 16" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12 16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="101"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="45"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ83128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="437" mass="49583" checksum="22B79CC56F91640A" modified="2017-01-18" version="1">MSIKNNIKSSEPHSEFNLQQLFKQMTEKLEKIVCNTISQRPLFQNSENINNNRDQDISLKNTKSSVKTNPVDTPNTNNSQNSFLMSPITTPLCKRFETLVVLFHISSIFVLSMFTLFVIANPFLLFVVFLPYFIWYLKDRTPANGQIDHFQFSFFRNLFIWRAYARYFPIKLHKTCDLQPTFPSPSPSSSSSSSSKNKASXSTDGPNYIFGYHPHGICALGSFGGLATNGCGFDQLFPGIKVSLLTLINNFQIPFYREYLMAAGLSSVSRKNALKLLQKKLSICIVIGGASEALHAGAGTSDLILLKRKGFVKLALEVGNVGLVPIYAFGENDLYTVVDVKKKSILYKFQLWSKKTFGFTIPIFFARGVFNYDWGMLPFRKPINVVFGKPIMVPKIENYTQQDVDHYHALYVXELETIFNENKKQFGYEDLSINYIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R982</accession>
    <name>A0A1E5R982_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ATP synthase subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3299</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Subunit b, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. Part of the complex F(0) domain. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. In yeast, the dimeric form of ATP synthase consists of 17 polypeptides: alpha, beta, gamma, delta, epsilon, 4 (B), 5 (OSCP), 6 (A), 8, 9 (C), d, E (Tim11), f, g, h, i/j and k.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the eukaryotic ATPase B chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R982"/>
    <dbReference type="FunCoup" id="A0A1E5R982">
        <property type="interactions" value="475"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R982"/>
    <dbReference type="InParanoid" id="A0A1E5R982"/>
    <dbReference type="OrthoDB" id="67388at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.2210:FF:000002">
        <property type="entry name" value="ATP synthase subunit 4 mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008688">
        <property type="entry name" value="ATP_synth_Bsub_B/MI25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013837">
        <property type="entry name" value="ATP_synth_F0_suB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12733:SF3">
        <property type="entry name" value="ATP SYNTHASE F(0) COMPLEX SUBUNIT B1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12733">
        <property type="entry name" value="MITOCHONDRIAL ATP SYNTHASE B CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05405">
        <property type="entry name" value="Mt_ATP-synt_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161060">
        <property type="entry name" value="ATP synthase B chain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0138">CF(0)</keyword>
    <keyword evidence="4 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ83093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="237" mass="26201" checksum="1AB0CD957BB61B78" modified="2017-01-18" version="1">MSLRVLTSKVATRSAMQMAARQMPIASTARFYSSAPAEDPKTKASSLINALPGNSVLSKTGLLATGTAAAIYTISNELYIMNDETILLTAFLGFCLTAAKLLAPGYGEFASNRVKQVTDILNASRNKHVDAVKQRIESVSELKDVTETTKVLFEISKETVELEAKAFELKQKVDIAAEAKSVLDSWVRYENNIKQEQQRQITEQVIAKVQAELKNPKFQERVLNQSVTEIEQLFAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA41</accession>
    <name>A0A1E5RA41_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2846</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA41">
        <property type="interactions" value="555"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA41"/>
    <dbReference type="InParanoid" id="A0A1E5RA41"/>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001123">
        <property type="entry name" value="CK1/CK1/CK1-D protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="75"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="398"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="399"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="444"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="460"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ83768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="634" mass="72278" checksum="5C5F2A78CA6F530A" modified="2017-01-18" version="1">MSRTSSTRNNNATNTNSGSTDTTVNSNSNHNTSTNNINNNNINNNNNNNNNNSKSNSSTKSKRSSQAQHVVGIHYALGSKIGEGSFGVIFEGVDLLNTKQNVAIKFEPRKSEAPQLRDEFRSYKILNNCKGIPQAYYYGQEGMHNVLVLDLLGPSLEDLFEWCGRKFSVKTTVLLAKQMIERLRDIHNRDLIYRDIKPDNFLISQYQRYQSSVKKCCISSKNDPNLVYVVDFGMAKQYRDPLTKQHIPYRERKSLSGTARYMSINTHLGREQSRRDDLESLGHVFFYFLRGSLPWQGLKAPNNKLKYEKIGHTKQASSPEFLCMNGKFPKQFGQYLQYCRTLKFDEEPDYNYLINLMDSVLEQSGQKDDQIYDWMSLNDGYGWDIRINKRSNLHGYGGAQNNNNYNNNNQNNVQYGQTQQHSAQQQRRASQQLSTSNQRRMLKYQQQQAALSPNTALPLQIQQQQQQQQQQKLMKLNSRTSGNNSXSYTSNYANYYLNNSKNPAVLDNEKSKLGMTSNAPYQQLQQQSALGSQQPLNTNANYYYYDSINNSGSNGPAGNIKRFSKQQQSRRASMRSAKNMNANSNAMTHPRAMAHGHAEGPGTVFSDDEYSHTTGYEDDEEDFKCCCCTNCVIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA49</accession>
    <name>A0A1E5RA49_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Large ribosomal subunit protein bL21m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3120</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RA49"/>
    <dbReference type="FunCoup" id="A0A1E5RA49">
        <property type="interactions" value="221"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA49"/>
    <dbReference type="InParanoid" id="A0A1E5RA49"/>
    <dbReference type="OrthoDB" id="3971598at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028909">
        <property type="entry name" value="bL21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036164">
        <property type="entry name" value="bL21-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21349">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21349:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL21M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00829">
        <property type="entry name" value="Ribosomal_L21p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141091">
        <property type="entry name" value="L21p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00044129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ83772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="154" mass="17842" checksum="22096A85405F8BFA" modified="2017-01-18" version="1">MFKNFFQKITTGLPSSGLRQFSTSKLFLNQVSTKPTLNVEPLKLSNELYAIFKIHNRPYLVTEGDKVILPFRMKNVEVGDILKLNDVTTIGSRNYKLIDYPIDESVYSLQAVVTEKTKRPMRIREVTKRRSRRTRHAKNKADLTILKISELKVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA80</accession>
    <name>A0A1E5RA80_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Vacuolar protein sorting-associated protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g2554</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the sorting nexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA80">
        <property type="interactions" value="780"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA80"/>
    <dbReference type="InParanoid" id="A0A1E5RA80"/>
    <dbReference type="OrthoDB" id="3972182at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030904">
        <property type="term" value="C:retromer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000022">
        <property type="entry name" value="Sorting nexin 3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015404">
        <property type="entry name" value="Vps5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555:SF170">
        <property type="entry name" value="FI18122P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09325">
        <property type="entry name" value="Vps5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="12">
        <location>
            <begin position="290"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="23"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="224"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="152"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="235"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="13">
        <location>
            <position position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ83816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="690" mass="77947" checksum="EF18507FFF2EBD1C" modified="2017-01-18" version="1">MSYDNPLEASVWDDNATSSKIINELSNTFAQSEVNEEPNWGESHNGSDQESEANEEHAPMPLDDQQNAPNNSIEAFQNQTXTNFARDAYSSNAFGETPFLASNPITGNIFATPESTKKSLLNELAPEEDPLAEIKEAAVTETDPLNGALFNDTDRSPLKSGVDDESLREKAGIPSLSKAHGSKPNRLFNIKRKTALRKNTKMAQPTDQNVFXDPLAKLVEQSEFVDEPLDDDXSTKQQSEVTKNYSGDKXQTVSESPQALNMNLAGKSLPKRPKKENTGENQDANLIFEPLYEIEVTDPTKVGDITSSHVEYKVRTKFSDGEHHTVARRYRDFRWLYRQLQNNHWGYIIPSPPDKQILNMFTTDLVETRRSQMSLMLNKIASIPVLNTDPDFVAFLKYDGAKLSTYSKSRNQDTLARSVNDANDLAEVHMSEITLFGHERGMDFYRLSGGADIESFLKKKLKGGGFLSFYAAPTPKYSEPDAYFSSQQESISLLKEQLKDIFKALELVQQEKLELASVTKEFAISLNSLIDLDVSANLTKVYRDFSQVHTNLQESLNRSSQMENLVLGSTVEEHLRTLSSCLAVFNQRQNLGTILVLLEETIQNMKITIAKAKGDKVVKQKEELEAFEQYHSIVKQSWKKISETIKXQMAFFDKQRIVEFRNSIEIYLESAIESQKEAIELWETFYQTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA96</accession>
    <name>A0A1E5RA96_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Actin-related protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3058</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA96">
        <property type="interactions" value="310"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA96"/>
    <dbReference type="InParanoid" id="A0A1E5RA96"/>
    <dbReference type="OrthoDB" id="5132116at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="142"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="334"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="400"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="151"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="334"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ83829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="548" mass="62217" checksum="38BB597E6D9E433F" modified="2017-01-18" version="1">MTVALADTQYRCCVVIQLGTTSTVVGYSNMEQPKXIIPTNYYHYAGKSSSDGINTKDINADTYRYGFYEILDELDENENKGDIFSIINSDYSKVDFDRLTGFLQYIYNDKLGLSSNELPLVVTLPYSAMYVNDYTRFGSGNCSNPTDETSNQNKNNHGNYNNQRNHHNGHKILSKYDKTPKYTLFYNKLKNLLLDDMQIPVINILNEPLSTSLSLGRVNSLVVDCGGNRCNVSCIIDGHVVSSISSKIAGNYLDYMIMEKINDKLKQMNITDSINSNNSVQQWKNSWLWLKDFKSLLLNCDPQLSVLNGSGVNGYNEYTSTGKAITNEMINTEHEHEQEHEQEHEQEHEQEHEQEQEQEQENTVNFLLKEQGGITLQFKESELKQLSDCLFDPVFSTTNTNTSTTTNQNNDNNNNQNNNQNNDNNNEQYFGVGKLIMTCIKQCVTETLAELSNTANTNDSNNTGSNTRSNELQEYTIIESLLNNVLITGGSTLLSGFENRILYEISTHYPKFKISTFANPVLQDRLLSNYHAGVAMSNLNWELNDWKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAC5</accession>
    <name>A0A1E5RAC5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein CAJ1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2761</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAC5"/>
    <dbReference type="FunCoup" id="A0A1E5RAC5">
        <property type="interactions" value="192"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAC5"/>
    <dbReference type="InParanoid" id="A0A1E5RAC5"/>
    <dbReference type="OrthoDB" id="552049at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000028">
        <property type="entry name" value="DnaJ domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026894">
        <property type="entry name" value="DnaJ_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052814">
        <property type="entry name" value="Peroxisomal_DnaJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45006">
        <property type="entry name" value="DNAJ-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45006:SF2">
        <property type="entry name" value="PROTEIN CAJ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14308">
        <property type="entry name" value="DnaJ-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="6"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="113"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="383"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="145"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="405"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ83856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="415" mass="46853" checksum="FBAD4C91ADF6DA73" modified="2017-01-18" version="1">MVKDTEYYDLLGIEPTATPVEIKKAYRKMALKFHPDKNPDDPTAQAKFQSIGEAYQVLSDPGLKSRYDEFGKEDAVPSQGFEDAAEFFSNIFGGEGFHDWIGEMSLLKGMSEDMEAESPTGTAASGEKSGEEGSKTTTNDGSVVQHDENFSKQQEKQQERKKLLEMEKKRREELEKQVAELTTALEKKIADYLTSVDIGNVEQFDNKLKKEVEELKLESFGLELLHLIAKVYKTKASNFIKASKTFGFSKVYTGMKDKKDTAKSMFTILSTALDAQQMMKDMEQLQLEEEDMDEYEKADLEKFITGKMLNTAWVMSKFESQSKLKEVCNNVLKNKEVSKKERLLKAKALLHFADIFGSAKRSPEEAEDARVFEELINDVKNQKKFKKREHSATAAKPAQEKLHTESASQASASAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAC8</accession>
    <name>A0A1E5RAC8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">General negative regulator of transcription subunit 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g2765</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CNOT2/3/5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAC8">
        <property type="interactions" value="451"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAC8"/>
    <dbReference type="InParanoid" id="A0A1E5RAC8"/>
    <dbReference type="OrthoDB" id="293823at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1020">
        <property type="entry name" value="CCR4-NOT complex subunit 2/3/5, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038635">
        <property type="entry name" value="CCR4-NOT_su2/3/5_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012270">
        <property type="entry name" value="CCR4-NOT_su3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040168">
        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007282">
        <property type="entry name" value="NOT2/3/5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007207">
        <property type="entry name" value="Not_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04153">
        <property type="entry name" value="NOT2_3_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04065">
        <property type="entry name" value="Not3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005290">
        <property type="entry name" value="NOT_su_3_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CCR4-Not complex component Not N-terminal" evidence="12">
        <location>
            <begin position="2"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="NOT2/NOT3/NOT5 C-terminal" evidence="13">
        <location>
            <begin position="913"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="266"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="361"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="457"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="695"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="266"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="280"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="316"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="387"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="410"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ83852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1004" mass="112715" checksum="9E4CFEB9AD53B6D3" modified="2017-01-18" version="1">MSHRKLQQEVDKVFKKISEGLETFDSLYERHEQCTNNQSQRDKLESDLKREIKKLQRLRDQIKVWQSSPDIKDKQILLPWRRKVENAMEEYKIVEKGSKEKAFSNISLKRSEVLDPHQQAIKDESDFISNAIEEFDRQFDLSQVEIDKVATLQRKRKNPSKSNEEKMKKLLNLQMKYRWHQEKLEIALRLLANEELSPDLVSSLEEDIQYYLDNNQDDPEAFIEDDSIYEPLHLDCNEFIAAEVAASFKAQHGSKLLEDAEKEELYQQEQKQRQLEDEQNLLNSKTNGNGVNGTESGSKSDHSAVPSQRNPLDPSKLSKKEQRKLLKDQKQAAKAAAKAAAAAAQSSMQITITEKSARNFQPVDIXDSGKSDTNGKVSEMAEEDQENDQHSREERARANKKLSELPISRTPSSATPTTLSASLSTSELAQKDTPVLNSSSSSLNNSFLPHIKAPAVNSRSSSIPLNNNPSRSISPEPTLQNHQQHQHITTLNGLTTTSTFKPAVTKPANPIAWSTLATKQHMQQQNQIQNPVQQQNPQQLNIQPILQPQQTIQAPLTPLTPQTQVKAEINWGKLAATKAKDTVQASSSHTTPATTVASLAGLPQQPATITTTTYSSPRLSHNNLVTSTTKVETTSTLNTSVVAXPPGMAPLSGVTGPSISTPANQHAQLNLSDNQSLPNNTNDADAEPNMANLINHNLNVQTQTLQQQQQQQQQQQQQQQQQQQPTLIQKTPSINGVPAQLFEPQLSENSILHGQNNEEMVFDDYVSECSXDELSVDEEIFSEHDALTKSGSIQSNNSQKDIAFLQKTQQVVADVFDDKTDYSLVALPSGLQDFVMSCISVDNSLXPSSKLQIRTGTQMCKPTRLPLGIPRNVVPPQQPNDIVRNMQQWDHARYXLGSLDGGIHSADSFXDEIISRFQNLDDFTLFYGFYFGLTPQEQSISEAVLKQKGWIPKWTRDQWCLKIGETKYEDQNCEIASYRVFKSEDWSCVDKANFKFEYAVMSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAL2</accession>
    <name>A0A1E5RAL2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cobalamin-independent methionine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Methionine synthase, vitamin-B12 independent isozyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g2978</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the vitamin-B12 independent methionine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.14" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAL2"/>
    <dbReference type="FunCoup" id="A0A1E5RAL2">
        <property type="interactions" value="592"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAL2"/>
    <dbReference type="InParanoid" id="A0A1E5RAL2"/>
    <dbReference type="OrthoDB" id="1053771at2759"/>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00082"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003871">
        <property type="term" value="F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03311">
        <property type="entry name" value="CIMS_C_terminal_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03312">
        <property type="entry name" value="CIMS_N_terminal_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.210:FF:000003">
        <property type="entry name" value="5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00172">
        <property type="entry name" value="Meth_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013215">
        <property type="entry name" value="Cbl-indep_Met_Synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006276">
        <property type="entry name" value="Cobalamin-indep_Met_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002629">
        <property type="entry name" value="Met_Synth_C/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01371">
        <property type="entry name" value="met_syn_B12ind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003556">
        <property type="entry name" value="PRK05222.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30519">
        <property type="entry name" value="5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08267">
        <property type="entry name" value="Meth_synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01717">
        <property type="entry name" value="Meth_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000382">
        <property type="entry name" value="MeTrfase_B12_ind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="UROD/MetE-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="5 18" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Cobalamin-independent methionine synthase MetE N-terminal" evidence="17">
        <location>
            <begin position="4"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Cobalamin-independent methionine synthase MetE C-terminal/archaeal" evidence="16">
        <location>
            <begin position="444"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="710"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="449"/>
            <end position="451"/>
        </location>
        <ligand>
            <name>L-homocysteine</name>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="449"/>
            <end position="451"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="502"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="579"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="617"/>
        </location>
        <ligand>
            <name>L-homocysteine</name>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="617"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="660"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="662"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="682"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="742"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ83946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="772" mass="86776" checksum="9C90E2E1356930E3" modified="2017-01-18" version="1">MVASSVLGFPRIGAKRELKKATESYWAGKITSEELFEIGSKIRTENWKLQKDAGVDIIASNDFSFYDQVLDLSLLFNVIPDRYTKYGLSPIDTLFAMGRGLQKPADEAKGIKAIDVTALEMVKWFDSNYHYVRPTFSHSTEFKLNGSKPVDEFLEAKKLGVETRPVLLGPISYLFLGKADKDSLDLDPISLLEKLLPVYIEVLNKLKEAGAKSVQLDEPVLVFDLPQNVQDAYQGAYEKLAKGVAGLDIVLTTYFGDVRPNLKAIANLPVSGFHFDFTRCPEQFDEVVAILKDSQKLSVGIVDGRNVWKNKFGASLKLIQSAVDKVGSDRVIVATSSSLLHTPVDLDNETSLSEEIKGFFSFAKQKLSEVVTLSAKINGSTDAAITKALEENAAYVEARNTSKIINNPEVQKRVAAIDSNMGKRQNAFPARWNKQKAIFKFPLFPTTTIGSFPQTKEIRINRNKFQRGKISAEEYEKFINEEIERVIRFQEEIDLDVLVHGEPERNDMTAYFGEQINGYAFTTNGWVQSYGSRYVRPPVIVGDLSRPKPMSVKESVYAQSITTKHVKGMLTGPITMLRWSFPRDDLDQKTQAYQLSLVLRDEVNDLEAAGIKIIQVDEPALREGLPLRAGAERNAYYDWAAESFRISTSGVADETQIHSHFCYSDLDPNHIKALDADVVSIEYSKKDDPSYISEFKDYPNHVGLGLFDIHSPRIPSKEEFIEKMGKILETYDAAKFWVNPDCGLKTRGWEETKLSLSHMVEAAKYFRGKYAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB02</accession>
    <name>A0A1E5RB02_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycerol-3-phosphate O-acyltransferase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2606</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB02">
        <property type="interactions" value="109"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB02"/>
    <dbReference type="InParanoid" id="A0A1E5RB02"/>
    <dbReference type="OrthoDB" id="2427554at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004366">
        <property type="term" value="F:glycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016287">
        <property type="term" value="F:glycerone-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052744">
        <property type="entry name" value="GPAT/DAPAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31605">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31605:SF0">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="480"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="531"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="573"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="652"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="707"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="743"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="652"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="750"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="779" mass="88396" checksum="2EB2AF06CF3F7913" modified="2017-01-18" version="1">MSNSNATAENPLLSERKKLEHQIIQTNQTNQAEHAEHAEHADHAVAGVVVKKDPFENYVYKPPHWYITFWYDCFLWIMTSIFDCFFREIRTRGAFRIPRGKTSVDGELTAAKGEDPIIFVAAPHANQFIDPVILMGRVKKAVNRRVSFLIAAKSLTRPAVGTFARFLMSIGVARPQDNLKLVEXGKVTVDPENPLKLIGDENCDFTVFKQKGLLGLPKSLGTANIEEIINKNELLLKKEFKMAKPEVSELLTRGTKIKYADPIDQSEVYAKVFEHLHHGQCIGIFPEGGSHDRPHLLPMKPGVAIMALGAMAKFDKLNVKIVCCGMNYFHAHKFRSRAIXEFGYPIEISRGLLEKYKNPTTMREAVQELMEQITEGLKSVTINCPDYETLMAVQATRRLYLGNMNNRMPLKVVIDMNRRLVQGYEKFKDDERIINLKKKVLEYNEELNNLNIADHMVGSIDQYNNWFKNIQILISRVVKILVLFLLSLPGFIMFSPVFILAKSISAKKAKEALANSTVKIKANDVIATWKVLIGMGFAPILYCFWSVLLSHYMCSKYQDTVTAFHLTKLKLFPLFYLASCVVTYCALIFDYGVENFKSLRPIVLLLRNPKGIKRLQKKREYLSAQITELVNDLGPQLFDDFDHKKLKNDYYSYDHEQEPNHSVDKNGLEKTGEYSDNVSGYESDGMSIFNSENHSISHIPIFPDRSRSNSVSSAAYHHPKSRSASVDSTFEIIESQDAPLDLVEDGFTAQNSSQDGQSSSVLSKKILSSIRNKRKEDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB87</accession>
    <name>A0A1E5RB87_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Aminotriazole resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2653</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB87">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RB87"/>
    <dbReference type="OrthoDB" id="2130629at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF14">
        <property type="entry name" value="AMINOTRIAZOLE RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="252"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="281"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="359"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="384"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="412"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="447"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="496"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="56"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ84156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="551" mass="60579" checksum="7FCF363DAAD9E884" modified="2017-01-18" version="1">MSSSRSSLTTTNESFKNTKKSYETQETNGNKQNAVHVSKLGPRPVYFKTTTSEIMFVMTAIISQIINQAGALQTLTMFKILSEDLNTDSKMESWLMASFSLVTGSLILITGQLGDIYGLKKILMYGYAITFVWSLILGFTRYTHNASFFIVSRAFQGLGVSCILPNVMGIVGNIYQAGSHRKNLVFSLVGAGAPVGAVLGVWWASFIVHFDVNAWSWCFWAYGILCFIILVLNHIFCPNGIPKNTKNIKMDWIGACLAVVGLILFNFAWNQGAIVGFQTGYVIALLVISCVAIVLFFVYEKDYAKYPIVPKXVFMNANVVFILASLLFGWGSFGCYLFYLFQRRMNIEDMDPLLAGTEFVSFAVFGVFAALTVALTIKKLTPQVLLVISTIAFCGGSIMFVCTPLHQLYWKLSFFSTLVLVFGMDFSFPAASIVLSDNLPIANQGMAGSLVSTMVNYAMSVFLGVAGVVETQTKKRISSKHPEYSEKRKTFLGYKAAMYFAIGIAGLSIVISSAYALKTLVLQKQKNAGSQEVLDFEANTSESNTSKASTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBI3</accession>
    <name>A0A1E5RBI3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Importin beta SMX1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2645</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBI3"/>
    <dbReference type="FunCoup" id="A0A1E5RBI3">
        <property type="interactions" value="301"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBI3"/>
    <dbReference type="InParanoid" id="A0A1E5RBI3"/>
    <dbReference type="OrthoDB" id="760868at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF28">
        <property type="entry name" value="IMPORTIN BETA SMX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="4">
        <location>
            <begin position="29"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ84257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="972" mass="110842" checksum="6E1D61FB7B33BA7F" modified="2017-01-18" version="1">MAALNLDSQVILERLQGTLQSDLAVIKQAEHDLYELQKQDGFCTELVGIVTNQELPAPSRISGCIYLKNKVQRSWPEKISLAEQSFLKDTLVKLLVQECNNNHLRPHLVETLRSILLVDQTWDLTDTCQQLLKSNDPQYTYAGILISFQISFIRRWDVEESRKYFDNFIESIFPILETMADTLKSSQDYQSNELLYLILKIFKYGCFISACPAYFKKDPQAFDRLITLFLELCKKPLPNEVMQLDPMDRSLDKRCKVQKWAYGNLSKMLSKYTRTTSSIDESMVNYVVSNTVPRVLEQYFQIMEIWSSKQLWLSESSLYNLITFLQKICVNDRLWPYIQPHLGTILNHFIVPCISSNEDTVLLYQEDPEEYIRKYFDDNKEDSDSASASSDFLYSVSHKRKEEIPTILKALRDIFASFDINNKESCYKQEAGLRILSTVNTFLTSGVQGSLTSEEFEGIYEHVLLPILALDGQYPFLVARALHCLSFTDYTFKNMDLLSKIFEQAYTKFISSSVEDDATLPLQIEGADALRSLILNNASIHDHIASFVPGMVEKLLKLSEAFEFEMLSQVIESLVENFSEQLKPFAAQLAENLANQYFTVGNTLVNGGDQGNDDQELLAASLISTMTTMVMSMPKVDMIKQFKPVVEFVIGNTQIEIMQDAMELYDSLINSSNDLYGALTEDIWNIYEAIVESFELYANDYFEQYETVFQSLCTYGFPKLPADKIQYVQNFVTIVDRKLDSCDNDFDITVAMNVLTFYTLSLNDIVLLPKCLDLYITYSAMGEDSPLDIEEECIIKLLLASFVVQPQQTLQLLQDGNTALKLFQAMFSCKFVSVFTLKLHILGTMNIFKLLKTAPTVVQGLLSQLSNSFISSIETLPKAIRKKDKMLKGEDIIDGNGFEDGANDEDDEDEFFDPYDDDFKPSAIDNVNVFVEIGSFFKQLPAAEPETYNAIISSLKPEKLATLKDVLEIVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBL2</accession>
    <name>A0A1E5RBL2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exocyst complex protein EXO70</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3110</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in the secretory pathway as part of the exocyst complex which tethers secretory vesicles to the sites of exocytosis. Also plays a role in the assembly of the exocyst.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Bud</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the EXO70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBL2">
        <property type="interactions" value="126"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBL2"/>
    <dbReference type="InParanoid" id="A0A1E5RBL2"/>
    <dbReference type="OrthoDB" id="1922221at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.170">
        <property type="entry name" value="Exocyst complex component Exo70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004140">
        <property type="entry name" value="Exo70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046364">
        <property type="entry name" value="Exo70_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542:SF41">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542">
        <property type="entry name" value="EXOCYST COMPLEX PROTEIN EXO70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03081">
        <property type="entry name" value="Exo70_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20669">
        <property type="entry name" value="Exo70_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex subunit Exo70 C-terminal" evidence="6">
        <location>
            <begin position="243"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="488"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="491"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU365026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ84288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="664" mass="75848" checksum="3E76BB38044E9A68" modified="2017-01-18" version="1">MIDPDEADILIYERGLQNAQKLTTNITKSITSIQESTYTCQKLFEPILLTNQRLATLSRNIEEXADSIASIKDVATDASKHDMNLSQPIEKIGLQKYFKSIYKLEDVLENLAESANXSNYLPXERILSNPNEFQGMSTNLQASVMNAEKKLQQYFLRTLNTLEPFDPQINMNKRSAFPYFEDAALERLNEIMTFFHGQNKYPELVEVLIQQRSEYIMKCMAFCEQFTKSLTQSEQSPYEKGESGVISYCEALTGFISNEQNLLEDLNIQNYDADSLSNVRTTLIKILSPVITSFIRVVHHNMSLVKRKPMLLGNFTFELNGCITKVIKSLNKISFLQQELEPLLKESEKFSLEILTSFIXYISQKASALSSLPTDFGITEVSVDIMSKLRKLSDYSKEISELFKKLNPPLQSWLGKPEPNWYKKYSSLSKQELTDPQNPNVLLGCYYGDCLDDLCVSLDLRATSILSSSNHKTHPYFQLYQPEHNLLNGPSGSSSASSSADGSGSNHGISNNEVKSLVGFFLFSNIFLIDQIVSRSPELTELLNSVKQRSPRLKKLKKYYTEQYLIKWKKCVQTIMSLVSNNMMNSQTAAPQQAKKQKEQAKELLKNFNXVFEILCSNFKNLRITDPALRKELVHEITGLVSPVYKLVWNKYQDTFKNREKYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBQ0</accession>
    <name>A0A1E5RBQ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein HIR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g2523</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Required for replication-independent chromatin assembly and for the periodic repression of histone gene transcription during the cell cycle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the WD repeat HIR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBQ0">
        <property type="interactions" value="450"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBQ0"/>
    <dbReference type="InParanoid" id="A0A1E5RBQ0"/>
    <dbReference type="OrthoDB" id="3972637at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000417">
        <property type="term" value="C:HIR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031120">
        <property type="entry name" value="HIR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011494">
        <property type="entry name" value="HIRA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13831">
        <property type="entry name" value="MEMBER OF THE HIR1 FAMILY OF WD-REPEAT PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13831:SF1">
        <property type="entry name" value="PROTEIN HIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07569">
        <property type="entry name" value="Hira"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0678">Repressor</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 10" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Protein HIRA-like C-terminal" evidence="12">
        <location>
            <begin position="742"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="455"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="633"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="637"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="658"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ84328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1033" mass="115338" checksum="A906E38D71D2F7DC" modified="2017-01-18" version="1">MKLLKLPEPPFGGNVTSMLTLQVQNSSATGVANQYLLTCSENCIIHVWDINDLKKASENSFEGIPQPKIILNDLELAKTTGCLDHTFNFKLLCTNVTNSKDGNQNTTLYAITDKNVYLLSHFEERIKTIELENQTPQNTSTKEESKPAIFSDNKYTLFELAENTEKKTIIDAQSVNEYLFLMVELIDSKLEKHYSIKVFKSQDIDALFEVKLSDIPASFIVDPHLDYFTVFNTNKSVKFYRFSISLKYGEFYSKLHSTICNVNPIHYKLNMAPQGNFIPLINAVTSSKVGSTLICDFNNKYKVVDSLITPTNSNFQILKYSPKVCVKPNKKNPTSMTIYNLIATTNXTDDSVMIWNTHRKKPLFQGSQITYSPTNKAISDITWSTDGMILFLGSKDGFISGLFFTQKELGVPMNASDFDTYSATYKSKLPPLKAQAALKKVSPNSRVNVKNLKATGKEETSKTASPIPVPNGNNAISTSAEEKLPNVISSNTMKFDPPSYKVPKDLKRVAPTLIASSTSADSQSTNANTKSNKKLKKELENIDFLDTSLLIPSVAFSKVRLATPKTRLHFEYKFLNKYRIRVTNGNSTELKPTKISLGSIKLKDKKNSFSNSSQPASAISDGFFETNMTKNGRVAETXGSDSSNSLNGNTSALDPISHQPNVVRSQSETPLLLETAPFLNPSLKSSEESATNPESSTATHEKPFVRHMHYIPKYVTICTGSNRFFAYATDNGYVHVHSVATGAKMXPPLILGVPLSFLESCGSYLSCITTTGQLYCWNIDSLKLKFPVTDVYALLSPTLRYSDDILSRAENITSYGVTNHGVPIVTLSNGDGHMYDEMMQSWMLINDSWWAYGSQYWDLTNTQMEYGGTVQSDGNDENSVTNGHSLSSSHTSNGILKYLESKTNNELHRRGVVKLLQKFAKTMLMKEGFENLEEVVTLAHLENKLLVLLKLKDLNDFENIMVTYCTKLSELDYQARLNEVFQWLYGVDGKDSLFDETLSKHALLKKVLIACAGIRKVQRLTLSYAEALNFELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDJ2</accession>
    <name>A0A1E5RDJ2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ribosomal RNA-processing protein 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2444</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRP12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDJ2">
        <property type="interactions" value="1061"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDJ2"/>
    <dbReference type="InParanoid" id="A0A1E5RDJ2"/>
    <dbReference type="OrthoDB" id="2192888at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012978">
        <property type="entry name" value="HEAT_RRP12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057860">
        <property type="entry name" value="HEAT_RRP12_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052087">
        <property type="entry name" value="RRP12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48287">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48287:SF1">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25772">
        <property type="entry name" value="HEAT_RRP12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08161">
        <property type="entry name" value="RRP12_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RRP12 N-terminal HEAT" evidence="6">
        <location>
            <begin position="61"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="RRP12 HEAT" evidence="5">
        <location>
            <begin position="359"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1007"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1051"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1052"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1104"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1163"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1185"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1203"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="7">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ84954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1221" mass="137860" checksum="651BEED2A1693EC1" modified="2017-01-18" version="1">MSQDQVANLMDLEDKFRHIRTQINSKLENQKHXAIILEAVDENIREQQKETKIEVIFNSSAVTNYLLSFLSILDQAVDQETREIKDLQLATSALYLLDIIFQYTPKKLLRSKFVDLLTKIAPCITDENSEAPIIRSAIGCLESLLVAQDNKTWVNKEQLNINPMRGLNAILELSLDGRPKIRKRSLDALHKILSNPPPPMTEHIISKPIADYSINALVTIVEENEKTQSKETSAMQIHICKLYSTIISTNQWPTSKLDNLIGLLLEVVKKNNGDTFLVSNVFQVFDSIFANENIDKVKLNAAMASILKLKPSIKDTIVATSWIGVIQKGCITLSKVSQEDFFYVELANVFRLMSEYLSSETASVNGKAATCLISIVNFGIQQSFLIDEAQDEMKIDQTVGSLANIIQDFLSSIRFSHSLXHVLHIATALISKLSLKSAPAFLPCLEIVGKWRSDENNSNQYVSECEQFIGASIQSLGPEKVLKVLPLNLGENANPNDHGRAWLLPLIRDNTKYAELATFIREFIPLITFFESKCEKLPEESIELRLFQTVVDQLWSTLPHFCWLPKDLTQSFSNEFASELSSFLYSKVDLRNTICHALRNIVTSNQEPVVDLVYKEDFFPEEVLASNLQYXSQTKAANILSVLFNVFAQTTATKRGFLLETIECYLKITSAEDLTKTFNNVCAMLKDALEKEDPNSSKRHDESKVSATLLDLVVTMAKYVPPASYDALFAIFNTTVASKDPLIQKRSYRIMSKLSELSSGTESISSYIGDIENVMFSQQTNCLTSARASRLSALKTVVELLPLQDLHFIVNVAPEVILCTRDVNEKTRETAFQILILMAKAMQASGSVIDLSKVASIPSDEKVPGDLMEFFKIIAAGMIGESQHMVTATITAYSCLVFEFKDVIDKNMLWEIYDTVELYLTSNSREIVKSAIGFTKVVVLTFPADEVREKVPQLLPKLLRWSHEHTGHFKSKIKHIIERLVRRFGEEFMEQIFPEEDRKLLSNIKKQRNRNKKKGVADEEEEEKDSKTAEAPVSTKGSRFMSAFDDVMYSDSDSDDDGEFQEFDEEGNKKKNKKSQQYIMENKENPLDLLDAKTLSHISSSRPKKYDSKRASKKEDYYQKDADGKLILNDGQTNKDEDDPLSSLTSGVNAYLEAVKQGPIRGQKNRLKFKKTKNNEADDFSDDEGSGKKPEPFAKKSNDFARRGGRVGKKPQNKFKARRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDJ4</accession>
    <name>A0A1E5RDJ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Protein ATS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2239</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDJ4">
        <property type="interactions" value="124"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDJ4"/>
    <dbReference type="InParanoid" id="A0A1E5RDJ4"/>
    <dbReference type="OrthoDB" id="5370059at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051553">
        <property type="entry name" value="Ran_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982:SF1">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="unsure residue" description="D or N" evidence="1">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ84980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="451" mass="50082" checksum="C53331BF85052838" modified="2017-01-18" version="1">MPVISIKCLGSNGNYQLGLNHDKDIDEPETTLRCYVKPELASDIYKPFLNGKELGSSEKPTDECDLXRLGFAYGSVFPVKIACGGNHTVVLLSNGAVIATGLNEQGQLILDSTVSENKVLRSWRXIYNDSAHPAIDIACCWESTVIAVVDQTQNSQNDFTDIHIKSFGTGMKGELGLGNKIXFNKDELRDVLALKSSSPISLLSSMANVIVLDKQAKVCYGWGNNTKNQLLLPEANENLVPTTNKLYKKRTKIVWEPVQIDKAEEFESNTFTLGKDFLAWVKKNSAHLTFEASGSLDMQTGLLXILKSKVDAMLSTKSEFQILKMESMWSSVHILFKQDNDTVKIMSLGKGRHGQLSMNDKLLHQYRDFSCGSEHGIFCNENNEVYCWGWGEHGNCGKRNPKRISEPHATGISQDXTVWENLNLVFCAESNEHVLFVQGGCATTFIVSMXL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDK8</accession>
    <name>A0A1E5RDK8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UDP-galactose transporter homolog 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2011</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleotide-sugar transporter family. SLC35B subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDK8"/>
    <dbReference type="FunCoup" id="A0A1E5RDK8">
        <property type="interactions" value="470"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDK8"/>
    <dbReference type="InParanoid" id="A0A1E5RDK8"/>
    <dbReference type="OrthoDB" id="1601at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005459">
        <property type="term" value="F:UDP-galactose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005460">
        <property type="term" value="F:UDP-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013657">
        <property type="entry name" value="SCL35B1-4/HUT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778:SF10">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08449">
        <property type="entry name" value="UAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="178"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="312"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ84966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="355" mass="39349" checksum="4944C0EB817F2B13" modified="2017-01-18" version="1">MPKPHNLQLIFLIAGIYSAFLTWGLVQEPLTTKTFPNSNEQFRFPFVISSAQSLVASLFGLIYLKWTGNKNTLSFKQLLKECYPFVALLSLTQSLSSPLATYSLKYIPYLTFQLSKTCKLIPVILVHLVIYKTKIPKKKQIVALIVTLGVTIFTMSNMKNKKTNTNENNVLQTTGNNALLSSASGYLLLGCSLICDGLTNATQDSMLKKQPKITGAHLMVLCNAAILFWNAVYLAVIDQQQLTNSWNVLRMDFNAISTLLATYAVCGALGQIFIFQTLSKFGSIILIMVTVTRKMVSMALSIVVYGHQLNRLQYIGILIVFAGITYEALAKRTASNGKALTSKSVSEEQTKKKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDW1</accession>
    <name>A0A1E5RDW1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2366</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDW1"/>
    <dbReference type="InParanoid" id="A0A1E5RDW1"/>
    <dbReference type="OrthoDB" id="5043642at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021848">
        <property type="entry name" value="HODM_asu-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11927">
        <property type="entry name" value="HODM_asu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="unsure residue" description="D or N" evidence="1">
        <location>
            <position position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ85098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="492" mass="57290" checksum="5F3224E2D3B60364" modified="2017-01-18" version="1">MSDAAILFLLKKQVTLPVLAYLLFLLKKDLFDHLXDSQICSTEVGFGVSAPSNNSISQNLNKENXANPYKYKPKTXNVFNNCLPIXNGQTYRWDQTKGYPYKPFKAGEYNLNMNVKPIEKELWLVVEXTYKRNIDAKWEIIRENYFDVVFFLDRDYKQTYTNAESEGHDFNEKDFEKSRRAVCEFYDHVVSFLLNRYPSAFEIVSAPVTETTHGSLPSNPGVLXNKIMNEYHPLDPWVYVSEPGSHWSKFLKFFCYETKPIIPNSIKIEEATWRYHVLILSLXRLIEEDYIILFPSANKRFNNEYCFFGGVFAFAAGFNPNDRYLKPLSLVHEPVPEYTTRLQNPMNKFFHKFKPGKPVMRVNYSFQTHSRLYVPDYNKGKETEYIKPKTSGELKGTLHYRSERQCVVKLSNTDATDESPSGGPAVFTIKTYLYNIVDEFFEMECYKSSDILQQLKQAIIDMQENVGQYKRKPEWGAGLVELINQKLVSQNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE78</accession>
    <name>A0A1E5RE78_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">J domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2169</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE78">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE78"/>
    <dbReference type="InParanoid" id="A0A1E5RE78"/>
    <dbReference type="OrthoDB" id="445556at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="89"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="410" mass="47755" checksum="ECA2F737F828D520" modified="2017-01-18" version="1">MGGLIGLSTRVSLAPILTHSGYNXFKICSIENKYSSFYLQKNHYATTSSSNQDHPEDTSKNTSNNRISKDINSLDFLIQKKWSLKENPTPYDVLYIENTREFTNRKFLRSRFHKLCKLYHPDIAGVDLMKDDNSLVTEEERLERFKMIKDAYTLLNTPSAKSKYDTYNLGWAYANNTNDSTSQSSSKSPYDLWKQRSADQDTHNMYWNAGNWEDYHNYHQRTGNPNHIAIHGKKLSHWDIIIYVLMLGALGESFSLLTDIQTSFGXRVDIHEQCEKDLIQSYLNYGLGDDYWARIRRFLWFRSFNLYSHSKEDLDREAIKNEKIIDALKGRHENNEQTSSPTNVKKTXKQVLQEKKLKNQKLKKKLQDIEESYAPKSTSKIYSKKMSADAITSSEHQNDHTQPDYAKSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE81</accession>
    <name>A0A1E5RE81_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Carboxypeptidase S</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2052</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE81"/>
    <dbReference type="InParanoid" id="A0A1E5RE81"/>
    <dbReference type="OrthoDB" id="3064516at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017141">
        <property type="entry name" value="Pept_M20_carboxypep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047177">
        <property type="entry name" value="Pept_M20A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE/HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962:SF1">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE_HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037217">
        <property type="entry name" value="Carboxypeptidase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="23"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="550"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR037217-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR037217-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="581" mass="64331" checksum="DFD794BD8EBC44EF" modified="2017-01-18" version="1">MANYVPIAASNAFKKLQAKKKQASLIFTSLLVLLVLVLSLYNRSTKDIPDYTQDCKSYSQLQPAYDVSLNQIFTDKVYQESVXEKLLGAIRVPTEIYDDSPNPDLSIPLDQDPIFKNFSSFHDYLENTFPLVYKHLTVETVNSAGLLFTWETKHTNTSAKPLLLMAHQDVVPVNKDTIDRWTYPPFEGVYEKKTGYIYGRGASDTKQLIIAQLEAIELLISTGWEPTKRPLLVAYGFDEECGGELGAQNIAKVLFNRYGANGLYGLLDEGGGTTHFGDGDYLAAPAIAEKGYVDTVITLNTKGGHSSAPYIWDFDQTSIGILSELNYFLTNDQFEIDIAPNNPALGWVQCKAKYDKTFSSELVKGWKSGSLKKLGNALKQNRLKFLFKTTQAMDIIHGGVKANAMPEQVXELINHRINTKSSVGETLDQVLSSTVSVADKYELGIILEWTNGTETSVLKEGTKGDFVITVKGALEPAPITPTGDDEMWNLVGSTVVNTFKNXFFGEDQDVYFEGTISTGNTDTKWESKSKLTNRIYRFSAALENPGSNAHTVDEKSSDKSLISALAFMYQIIGNVDAYSTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RED4</accession>
    <name>A0A1E5RED4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">tRNA (Cytidine(32)-2'-O)-methyltransferase non-catalytic subunit TRM732</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1695</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the THADA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RED4">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RED4"/>
    <dbReference type="InParanoid" id="A0A1E5RED4"/>
    <dbReference type="OrthoDB" id="73997at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056843">
        <property type="entry name" value="THADA-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056842">
        <property type="entry name" value="THADA-like_TPR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019442">
        <property type="entry name" value="THADA/TRM732_DUF2428"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051954">
        <property type="entry name" value="tRNA_methyltransferase_THADA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387:SF0">
        <property type="entry name" value="DUF2428 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387">
        <property type="entry name" value="THADA/DEATH RECEPTOR INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10350">
        <property type="entry name" value="DUF2428"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25150">
        <property type="entry name" value="TPR_Trm732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25151">
        <property type="entry name" value="TPR_Trm732_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA (32-2'-O)-methyltransferase regulator THADA-like TPR repeats region" evidence="4">
        <location>
            <begin position="251"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2428" evidence="3">
        <location>
            <begin position="636"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA (32-2'-O)-methyltransferase regulator THADA-like C-terminal TPR repeats region" evidence="5">
        <location>
            <begin position="863"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="6">
        <location>
            <position position="1293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ85226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1420" mass="161337" checksum="215A73482736F84D" modified="2017-01-18" version="1">MNSKKICEYLQTDSLKEFTKQCHCLMPSLDIKTVKVNLINLNVAQDKNENLNASLQVLSETFPVLFQRLQELKGDDPKQRTLINDALTIWFVKASKYPSKXFSGLEDNPVSIYGTLEKLDCYTFIFQYTIDFWIEGNSALSNSTRSLFIKMCTFIEXFEDLNGLLFKKWLXQCLYDINKKMKVRYFLFDTLFSFMSTTQAELVIFEDNHSNFITELLELMSSNALALPAGKTLSNFIIRLYKEKCEEKINQHWINQTIECLKKSEPSTVKNMELYLLSPIFKKVPKSVFKTFVSRLKNDISTMLSILKVGQELGIIDEPFHPGEYHLISLDVVVNELLQSSLYKNSAFQLLTYNCKSSQPLQGYLYEIILSNITVFFVDYDVWSRNYFESLFKQFLYRIKDSTYGLNRTVQKLRAKNKFLEEQIAKAKDIVQAQSFILKLMGVVKFQLIPGSQYQRILVALKVLKILLESGLDSRIEKKFYDKKQATTSWCFNXDLVDESLIKLLLNTLTNSFTDVQILSTNLLIMISSSEDLSNLAARHIDTLCLFQTGLQMLKSYNSTVSGSRVISFVVSSLLNSNKISMLHDCICQILVELENSIQISNEDYLKGLDYPVDGYFLSLSSIMENDSSAFETDTISKMVEAVKNNWVSTKGILCHDSSEGSMPLEYLNTGISDQIITSYAYRSVKEASALLETLLLSQKVLTTDQLAEVGSLLMEQLNLTRHSGAFQAVAPSFSACCAAISNTNNNKLSCWLDEILQSMQNKAQHITRRSGGLPFLVTAILSSEPLKNERPLLHYTFRELLSIAQVPILKHEEKLDLPQVHALNSMKAIFEEPKLNDASSKYVYEALILCLNNFQSSLWSMRNCAIMLFTALQNKLFGKFGKTTSARSFFTKYKGIRESLLEHLSNAVDSETGSSVEILFLVLTIISKLKQTPGYDGLDAFVPLVVQCLESATWKVRELAARAMPGIVQDTTEMSKYLLKTLTTKPLSSQNRLHGYLYAINEMKACGVTFXSSTKDSVYSLFHTCVFSNPCYSSANEYVNLISALNLQDDSTFFNNLRWFFVERYGDETMYGSKQLLLKNIAELLLKYEDPANIEDLLMCILGSTSFEVQLSGLDFIXENQKSYVSVTSMLYEMLNNDSTWMYVKSKILLALQACGKSIPSTALLKLLESETESDDVKLSALQSFSSFTDSEECGKYWNLITEYSSDAMDLPYRKAALCSLINLPVSSQSLLPLSLFLNDDDYDLRAVATEFISSKYLKDNTSDIPKTVFSRFFEKYNENNARETTEYLINDLKQMQVDLVANSKDSDQLFALEQPNMVKNVPEMIFAISRKLDVTSTENYPMFEKELKKNVLKLVDQLAHLEKQDQLIAWCSSAELFNQTATLLILSKRFDALSFKKLTDALGTASCQYVLDSLSFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REE3</accession>
    <name>A0A1E5REE3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">37S ribosomal protein S16, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2443</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REE3"/>
    <dbReference type="FunCoup" id="A0A1E5REE3">
        <property type="interactions" value="770"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REE3"/>
    <dbReference type="InParanoid" id="A0A1E5REE3"/>
    <dbReference type="OrthoDB" id="407221at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1320.10:FF:000013">
        <property type="entry name" value="Mitochondrial ribosomal protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00385">
        <property type="entry name" value="Ribosomal_bS16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000307">
        <property type="entry name" value="Ribosomal_bS16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020592">
        <property type="entry name" value="Ribosomal_bS16_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023803">
        <property type="entry name" value="Ribosomal_bS16_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00002">
        <property type="entry name" value="S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12919">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12919:SF20">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS16M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00886">
        <property type="entry name" value="Ribosomal_S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54565">
        <property type="entry name" value="Ribosomal protein S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00732">
        <property type="entry name" value="RIBOSOMAL_S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="126" mass="14407" checksum="FFCAED8CF5149AE6" modified="2017-01-18" version="1">MAKGLVRIRLARFGRKHTPKYNIVVTHNTKARDAKPIEVLGTYDPIPKPRTMKEIKNNVTPVKEIHLDMDKSKYWVSVGAQPSDTVLKLFMKCGILTPDFAKNKKFYPANKDVESPLKIMDQVENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REG4</accession>
    <name>A0A1E5REG4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Carbamoyl phosphate synthase arginine-specific small chain</fullName>
            <ecNumber evidence="3">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Arginine-specific carbamoyl phosphate synthetase, glutamine chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Glutamine-dependent carbamoyl phosphate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g2101</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Small subunit of the arginine-specific carbamoyl phosphate synthase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer composed of 2 chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CarA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REG4">
        <property type="interactions" value="361"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REG4"/>
    <dbReference type="InParanoid" id="A0A1E5REG4"/>
    <dbReference type="OrthoDB" id="434at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005951">
        <property type="term" value="C:carbamoyl-phosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000016">
        <property type="entry name" value="Carbamoyl-phosphate synthase arginine-specific small chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.20:FF:000003">
        <property type="entry name" value="Carbamoyl-phosphate synthase arginine-specific small chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="entry name" value="Carbamoyl-phosphate synthase small subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01209">
        <property type="entry name" value="CPSase_S_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009475">
        <property type="entry name" value="PRK12838.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF4">
        <property type="entry name" value="CARBAMOYL-PHOSPHATE SYNTHASE ARGININE-SPECIFIC SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405">
        <property type="entry name" value="CARBAMOYLTRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="Carbamoyl phosphate synthetase, small subunit N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carbamoyl-phosphate synthase small subunit N-terminal" evidence="16">
        <location>
            <begin position="7"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00057756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ85285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="406" mass="45331" checksum="2D2504C3ABE0A10B" modified="2017-01-18" version="1">MFSEENHKAIFKIKNGPTFEGYSFGHNSSEIQSGEVVFTTSLVGYPESMSDPSYKGQILVFTQPLIGNYGVPSDTEKDEFNLLKFMESPHLQCKGIVVSDYAWQYSHWTAVESLQQWCVREKVAAIGGIDTRQVVQLLRDQGSTLGCIITEDIEKHADLESIQYEDPGLQNLVQVVSTKAPYYIKSIQEXPLNVLLVDCGCKENIVRCLTSRNSNVTVVPYNYPIDEIAASFDGIFISNGPGDPKHCQITVENLAKIMKTDVLKNLPIFGICLGHQLLCLAAGASTKKMKYGNRAHNIPTMDLLTGQCHITSQNHGYVVDETTLPENEFKPYFVNLNDKSNEGMTHVTRPIFSTQFHPEAKGGPLDTSYLFDNYFQNIKKYIATRTFVAEVPASLIPQNLPTERVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REI7</accession>
    <name>A0A1E5REI7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF3074 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2436</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REI7"/>
    <dbReference type="InParanoid" id="A0A1E5REI7"/>
    <dbReference type="OrthoDB" id="6423603at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024500">
        <property type="entry name" value="DUF3074"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40370:SF1">
        <property type="entry name" value="DUF3074 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40370">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11274">
        <property type="entry name" value="DUF3074"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3074" evidence="1">
        <location>
            <begin position="87"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="286" mass="32760" checksum="613F57B7903762CD" modified="2017-01-18" version="1">MSFLSSANLFNKTTLPVSHENIVQTIQTFTDNIFLXRDGATSGHKWDRGSDYHLDVPAKQGPAXTMTKQLVETYNTYTNFDGQSDYWSCRISEHKSIDEDLYREKIVGNLNGSVFKKETGEWELPEKLSYRCTNEKNYFHAIESVTVLHDNVFLESASAENVSSPSGGWVEIEALYKFSPVLKKRVFNEWVYVVKPFKVSGDSSTEVSYVLSIRSDHNQDGHKSEHVPAYYVSIEKLQYNYTTKQFTWLMCTASDCKGIVPQFVQNIMINKAVVHDVPLFFSYIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REL4</accession>
    <name>A0A1E5REL4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REL4">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5REL4"/>
    <dbReference type="OrthoDB" id="5329385at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031342">
        <property type="entry name" value="Mug163-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17119">
        <property type="entry name" value="MMU163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="346" mass="38662" checksum="E36B7E63450000D1" modified="2017-01-18" version="1">MSSKPFAVVALSTHVRAGQLGPLALSQPLRMLVACTVSRSTTALSSFGQQRFNSTLGNEARKANYHELKQFIQYKYVPTLFNPNKSLSDFFHXYQDKFDQHIALRIFPSTIGIIPHFKGMLYYKSLLITGKLLVLNNGSTXKLTKAFFQKQFEHFSEGDSKHSSGNTVNESCREKPLQEAQNNERDSLPFGQTDLKEDQEKVKLKILQVEELFMGSNNKGKCLDYNVQDTVSDKLVIKWELNLPGFDTSKQIPTSSASDSASNIVNRGKANASDFAAGSNEKPEGFSSNGSASDSPAVRKKQCITGLFIFEFNPDNTKISVHTIDHVVFDNDKENKKQDSVNLLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REP4</accession>
    <name>A0A1E5REP4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sporulation-specific protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REP4">
        <property type="interactions" value="119"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5REP4"/>
    <dbReference type="OrthoDB" id="376826at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17316">
        <property type="entry name" value="Perilipin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="325" mass="36086" checksum="0E68EC0E78FE1AD6" modified="2017-01-18" version="1">MSAESIHKKIIEPITDTVGTASATVSETAAQVTTNITANISESPIPMKTLDHLYSYPLVQQTTEFVGDIPAVRILNANLLPLIDNKLVAKAFTVVKPCTTLVDNMGEKSLQIVDKVVPSLKEKTYQKLGEEVAYPYTYTKETVQKGTQLASTYATEYCYEPVHSRILGFRKFYNEKLYDTKGKXLIRGSLDPFVGPINGKLEQYAEQYLPKGNKVSTDGFSNEVDRSFAIVWNALGRLIPATEQKVGDIVTAPCQYTKHVCETFNHNLDQEKDLGLKNSFNASKKSVQELEKEIVDSVKEXNPLKSKTEKKKESLLDSIMPTAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF11</accession>
    <name>A0A1E5RF11_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cold sensitive U2 snRNA suppressor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2097</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF11"/>
    <dbReference type="FunCoup" id="A0A1E5RF11">
        <property type="interactions" value="216"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF11"/>
    <dbReference type="InParanoid" id="A0A1E5RF11"/>
    <dbReference type="OrthoDB" id="10260794at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007180">
        <property type="entry name" value="DUF382"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006568">
        <property type="entry name" value="PSP_pro-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052584">
        <property type="entry name" value="U2_snRNP_Complex_Component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12785">
        <property type="entry name" value="SPLICING FACTOR 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12785:SF6">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04037">
        <property type="entry name" value="DUF382"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04046">
        <property type="entry name" value="PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF382" evidence="2">
        <location>
            <begin position="143"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="PSP proline-rich" evidence="3">
        <location>
            <begin position="288"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="477"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="517" mass="58925" checksum="2BE9287FD9571DA2" modified="2017-01-18" version="1">MARGNRNKNKNNKTLCADENRLSLANLIATDDVEKLDEEQLKHSFHISKNLQIDHQKDGALKTKSGNMDIAKNIPSTINHRVSKKPKKKSPKKVTELVAVKMQDTSTELHKDRTERAEPFEKKKNHTPPSLLELKTYVSGFSELIQDYDVTSTNPFTNAAIKTNKNAVDVPENWKQKRGYLTSRSLMDKPNFELPDLMKQTGIQELRQIDPFDDPNGGNNNSLKEETRAKIQPKMNVLDLDYKKLYDVIFKIGANWKPDNMLQFGDLYYENREMDFNNTLEGYDKRFRPGKLSESLLSALGLHHNGRLPAWCIMGDATKQKQGGKGNSNKKTETAKPGSSFGEHINCFNNHYTLNVLNKIDKDGVYAQLMRKRKKGAFAGKAGKAGRLNTDNKENGLFGAKINISIELDDVDLKSQENEKDIPEEITKQQVQEQVTELAVNDTITTETVAKDQHVQKEASKKLFEVMQVQSLQDGKHAYKLDRCEKDEQKLKPSKLKTDEISDHKEKEISEEQEFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF19</accession>
    <name>A0A1E5RF19_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">18S rRNA (Guanine(1575)-N(7))-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1721</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class I-like SAM-binding methyltransferase superfamily. BUD23/WBSCR22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF19"/>
    <dbReference type="FunCoup" id="A0A1E5RF19">
        <property type="interactions" value="999"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF19"/>
    <dbReference type="InParanoid" id="A0A1E5RF19"/>
    <dbReference type="OrthoDB" id="2877at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016435">
        <property type="term" value="F:rRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070476">
        <property type="term" value="P:rRNA (guanine-N7)-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000017">
        <property type="entry name" value="probable 18S rRNA (Guanine-N(7))-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039769">
        <property type="entry name" value="Bud23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022238">
        <property type="entry name" value="Bud23_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12734:SF0">
        <property type="entry name" value="18S RRNA (GUANINE-N(7))-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12734">
        <property type="entry name" value="METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12589">
        <property type="entry name" value="WBS_methylT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="10">
        <location>
            <begin position="52"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="18S rRNA (guanine(1575)-N(7))-methyltransferase Bud23 C-terminal" evidence="11">
        <location>
            <begin position="223"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ85500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="271" mass="30679" checksum="1177FD9E40C983A9" modified="2017-01-18" version="1">MSRPEDLAPPEVFYNESESSKYTTNTRVQHIQAKMTLRALELLNLEKSSFILDVGCGSGLSGEILSEDNHVWCGMDISPDMLQQGLEREVDGDIMLQDMGQGIPFRAGSFDAAISISAIQWLCNADTSYNDPKQRLMRFFNTLFGCLKKNGKFVAQFYPKDDSQTDQILQASKIAGFSGGLVVDNPESKKNRKYYLVLTSGSGSSDINLQGATMDENNANDLKLQRRLKKNKKLQESSKNFIQRKKELMRKRGRNVAKDSKYTGRKRKYRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFE4</accession>
    <name>A0A1E5RFE4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Diphosphoinositol polyphosphate phosphohydrolase DDP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1834</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFE4">
        <property type="interactions" value="242"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFE4"/>
    <dbReference type="InParanoid" id="A0A1E5RFE4"/>
    <dbReference type="OrthoDB" id="3971373at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034431">
        <property type="term" value="F:bis(5'-adenosyl)-hexaphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034432">
        <property type="term" value="F:bis(5'-adenosyl)-pentaphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008486">
        <property type="term" value="F:diphosphoinositol-polyphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000298">
        <property type="term" value="F:endopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901911">
        <property type="term" value="P:adenosine 5'-(hexahydrogen pentaphosphate) catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901909">
        <property type="term" value="P:diadenosine hexaphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901907">
        <property type="term" value="P:diadenosine pentaphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071543">
        <property type="term" value="P:diphosphoinositol polyphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04666">
        <property type="entry name" value="NUDIX_DIPP2_like_Nudt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047198">
        <property type="entry name" value="DDP-like_NUDIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12629">
        <property type="entry name" value="DIPHOSPHOINOSITOL POLYPHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12629:SF0">
        <property type="entry name" value="DIPHOSPHOINOSITOL-POLYPHOSPHATE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="5">
        <location>
            <begin position="27"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ85621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="201" mass="23206" checksum="1E5760764ABA9BCD" modified="2017-01-18" version="1">MQSSQVFDKSAAARVGRDNQVYAEETGARLVAGCVCLNEDRSKILMVQSSAHKKKWVLPKGGVEKDEYPYYNKTAIRETWEEAGCIGDIVKDLGIVQDMRPPKKWGNLEKFNNHDNNSNNHNDIKKVGLAVEEXQAKDVPIPPRSEFHFFQMIVKETPNIYPESHKRKRKWFDYSQCIEELTRAKRPELIECVERSDILKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFG6</accession>
    <name>A0A1E5RFG6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 12">Adenylosuccinate lyase</fullName>
            <shortName evidence="12">ASL</shortName>
            <ecNumber evidence="6 12">4.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">Adenylosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g2074</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>(2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + fumarate</text>
            <dbReference type="Rhea" id="RHEA:23920"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>N(6)-(1,2-dicarboxyethyl)-AMP = fumarate + AMP</text>
            <dbReference type="Rhea" id="RHEA:16853"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer. Residues from neighboring subunits contribute catalytic and substrate-binding residues to each active site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.2" evidence="6 12"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFG6"/>
    <dbReference type="FunCoup" id="A0A1E5RFG6">
        <property type="interactions" value="727"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFG6"/>
    <dbReference type="InParanoid" id="A0A1E5RFG6"/>
    <dbReference type="OrthoDB" id="406045at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00132"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00336"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070626">
        <property type="term" value="F:(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004018">
        <property type="term" value="F:N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03302">
        <property type="entry name" value="Adenylsuccinate_lyase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.60:FF:000001">
        <property type="entry name" value="Adenylosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.40.30:FF:000005">
        <property type="entry name" value="Adenylosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019468">
        <property type="entry name" value="AdenyloSucc_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004769">
        <property type="entry name" value="Pur_lyase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00928">
        <property type="entry name" value="purB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43172">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43172:SF1">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10397">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00998">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 12" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Adenylosuccinate lyase C-terminal" evidence="13">
        <location>
            <begin position="374"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00017058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ85648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="482" mass="54022" checksum="6377B7A0A9315B2C" modified="2017-01-18" version="1">MSENDKYTTPLSSRYASEEMSAIFSLRNRFSTWRKLWLNLAIAEKELGLNVITDEAIEQMRKNLNITDEEIAAASKQEAIVRHDVMAHVHTFGETCPEAAGIIHLGATSCFVTDNADLIFLRDAYDILIPKLVNVINRLADFALKYKDLPVLGWTHFQPAQLTTLGKRATLWIQELLWDLRNFVRARNDIGLRGVKGTTGTQASFLSLFHGDHDKVEALDKRVTELLGFDTVYPVTGQTYSRKIDIDVLAPLSSFAATAHKMATDIRLLANLKEVEEPFEKSQIGSSAMAYKRNPMRCERVCSLARHLGSLFADAVQTASVQWFERTLDDSAIRRISLPGAFLTADILLSTLLNISSGLVVYPKVIERRIKGELPFMATENVIMAMVEKGASRQEVHESIRVLSHQAAAVVKEEGGDNDLIERIKKDEFFKPVWEELDSLLDPSTFVGRAPQQTEKFVANDVANALKPFQDKITDAEVKLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFI0</accession>
    <name>A0A1E5RFI0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">TY1 enhancer activator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2215</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFI0">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFI0"/>
    <dbReference type="InParanoid" id="A0A1E5RFI0"/>
    <dbReference type="OrthoDB" id="3970540at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051615">
        <property type="entry name" value="Transcr_Regulatory_Elem"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31313">
        <property type="entry name" value="TY1 ENHANCER ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31313:SF81">
        <property type="entry name" value="TY1 ENHANCER ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="53"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="119"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="831"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="925"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="19"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="119"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="144"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="169"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="839"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="10">
        <location>
            <position position="1046"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1129" mass="126126" checksum="923BC5940C838BE9" modified="2017-01-18" version="1">MDDKQPASIKGELSPSVEPFNSHQNTNSNFQPIGNTNNLGTEEMEKGTKKRMACTNCRKRRKKCDFQYPCSSCLKLSLDCNINVNDLRKKRYKNEYVEELESKVQFLEKKLLETQNLKSSSFANSASPASSSSFNAISPSNSNGILQETRNSPLHPNTFEHKHLKPQTSISSLPSLSNPGSKKSSSRINDLKTTVIVRPDAPLDSKVSQNSKENNAPLVLDHGQMSSTDTKLHPIIPSLNSLGVKNSFTNGSNNTGSIYPGTRPTSSGGFQLPQPPLHLLHASKPAHLSNDSQILQSLKLFFQWLYPGYFIFVHRESFLYGFFTDYQDGYEHSRYCSFELVYAVAAIGSRLSPELRSKSAEYFETARIEVLNKIFQQSYTAEITTVQTLMCLAFYELGNGNFSSAWYFSGLAIRIGHDIGFQLDPQAWVVESGKNGFEDDTVGNKSSYNSMGQAGHSSEDDFNDSDKLTRSEMAIRSRIYWGCYVADHYICLILGRTPSLSVSNSTIPDSDEMPEIAGTEDFKFESKLILQVSLPLKNLIILSRIVQIFTSKLFIEPNISKLEKLEYLRKFNSKVYSWRQSLPNFLKWSKNTLETNLDYSTDPTICYFWYHYYIVLLTFNKPFIGDSEVSQVAVQEVLDDLKMLFTNFKLKMGNDCLNKCSLNQLFSCSLAIQILVKLKTTIVTRAELQKTRNDERGLKLEKKLQDIDESLKFFSDMFELMSPTYDIPGKMIGGTLSRNTKGNTYLLRSKDSLNMIKKSTSVPPPFIGTKTGTMGLSPGGSGALQEYAHAPSPFAMTEKMQFANNGSTHPSPQPPQLSFFKTTNLPIKRPFITTGHTRSSSQSQLPSLSPQPGPQYQQPPSHLQPQRNTSIPCPPSQPSASVVIGMNVNNQYVNNVNDLNNMISYHHALKRDDMGPVLTTAFNNNNNXNNNNNNNNTXTNTNTNTNTNTNTNTDIIHDQNFSKISSKEILGPSLEQPDLDLSTALTPIANTTSDVVKTTINNSVNNXVGDTIKKMPLMSTFKTTTSNRKTFGLNELRDSDTTMLSDNTITGVPNANNTVSNLTNLTNLTNSXNPNGNSNHTPHSNNNVNTLFINNNNIEYTYDFSLSDEIDGIIRETFGIDNFNTHHTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFJ9</accession>
    <name>A0A1E5RFJ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Signal recognition particle subunit SRP68</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1845</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SRP68 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFJ9">
        <property type="interactions" value="966"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFJ9"/>
    <dbReference type="InParanoid" id="A0A1E5RFJ9"/>
    <dbReference type="OrthoDB" id="10255118at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030942">
        <property type="term" value="F:endoplasmic reticulum signal peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005047">
        <property type="term" value="F:signal recognition particle binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3450.40">
        <property type="entry name" value="Signal recognition particle, SRP68 subunit, RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026258">
        <property type="entry name" value="SRP68"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038253">
        <property type="entry name" value="SRP68_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12860">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 68 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12860:SF0">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16969">
        <property type="entry name" value="SRP68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0733">Signal recognition particle</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="606" mass="69228" checksum="6E6A48BAAD4A8B85" modified="2017-01-18" version="1">MXRFYSPLSQTFGSRPDDFSYSAGSDFRKTHLKLNHKLVKLRQYCHVLNKNTKSYDSSKLRQFSAETYAKENKLQGALVLHLIERDLVYVENLRLNHRVGKKNQRVNTRLRKASKYAAQLKQLVRLEKVWQTKLEYLIYSQLADIEYHSTTSKKSGASESEISLKYFKVLMGLDLLLQDRKAKNIDEDYKIFEDIVEKIKAKYRYIPLPSTVQHKKDVTKLLSHSKKGTAKTCLIXQILNSNKYRPNYYFLGVTSEAMKDKKYIKXVEWRSFKADVNDEDLAKHLTNALNLQNKKLVKSRQDGNAAASSQDENELYDEMLMNYSLAEEHHQKIMDPEDNADQILSTFISYNKLFVMIQRDALIFEHLLKSWXNAANCGKIKYQEIGEVYAKLEKIVKNLSATLTQVTXLPGVYSDDDLLGNLRLSXAFYKSSLYAYCLAPLYKIKGQYVESMALLNHSLNALNECSAFNSNLSLPGNIVNQRKVSSLKKNIEKACSDVESLAVYQQNLRTKASQNCKYNNGVTVVESGFKISPSLVELNNLFPLKPTIKPVMAKPTLFDLAYNYIEFNDPLANNAPKEXAAEVKAAEPVGAEPLVQKKGFLGFFRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFK8</accession>
    <name>A0A1E5RFK8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CUE domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFK8">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFK8"/>
    <dbReference type="InParanoid" id="A0A1E5RFK8"/>
    <dbReference type="OrthoDB" id="5577209at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="443"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="543"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="587"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="609"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="2">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="621" mass="70930" checksum="F16AF68A32B2056D" modified="2017-01-18" version="1">MSKVLEIDSNPEESLVNLPIVTFPPFKLRAALVEKDPVVWVHLLEAYNMFMAHLIQHRELMRLNEKTWEQLCIFIRSFLSETSSEMGQLLSLGSNVDVTRNLEHLKRWVLKLIEVGGGAHKLQMNAGTLWDFVRVYVETDANAVRKVIAEGGKIDCTYMLHNHLEFLVGSGKFDRYDLKTFENLIDRKSTTRSTTQSVLTKNNAEKTQINGKISQRAKKYDSNKFIDKFVTVKWLEVLEGLYNHGEGVHSNTCKQLMIITLLNCSIQNVSSLATQLGVSNLPALKLYPLFGGVLWAGFTEKXYFKGLETRLPFLKASAMNGNAQSNGKSHALSSNSKQLPHTVDIETVRQVQEVFPELPVSKIQQLCPRFESAEAFINALFENPSFIEDLEAQNTDFLKETIGLDEFKNKLNIXKKKNTNKIFDRGVPDEVRNKTMTAALRLLYDPNEDERDDTYDDAERQQPGNGADESNKETEKYNKTEEYLWNLLKKDAQLFAKSSRKSNSRKDLKNKTKWSDEQIEGWARMIERSPARALMLEEKYMFQNPNRTPITNEKDXEETEEDASNNXXXDKKKGKGRGKDKKNDAATESNQNETSNSSDKKNKSNXGSHNRRAQYSRGXVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFM6</accession>
    <name>A0A1E5RFM6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ASTRA-associated protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFM6">
        <property type="interactions" value="57"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFM6"/>
    <dbReference type="InParanoid" id="A0A1E5RFM6"/>
    <dbReference type="OrthoDB" id="7668193at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ85646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="431" mass="48624" checksum="5EFD046B827A65F3" modified="2017-01-18" version="1">MLQVEQFTLRHHTSSVKALAVYQKNSCVKKCSRLYTADEHGNICEWDLPKRKLIRSWSVCDSKALQICIXGIGILKSDLEXVLLYVHSKDHCLRFYDVTVSQPQKQSXYLPKPQLLWEMPVNTLNFSNVVVIPCSTQKTKKGSFQVWCCNTEDTEGIDVYEINPITKNLKRXVKNKKFGENVKRGIVMCICATKDFEYISVGYENGSCVLVERDSVYTNSNXHIDVPFDXNSHFEQPILSMSCSKTSLYIGQAKSSSLYEYXLKTGVCETINIGKSAGSQHFARRELSVTGICADLYETHNLIGFTTWETNTYYLFNVEHKXIIXTLEKSKSNVVPVSLDPTGNIETXGSNDLSEFEKSNNVSIESCLMMLGQKNNSTDSEKSNSQNXVLXTQRSRAIGANPLAMKRINYMLQKDWLFLGFADGSVTVSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RG14</accession>
    <name>A0A1E5RG14_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CCR4-associated factor 16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2400</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RG14">
        <property type="interactions" value="421"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RG14"/>
    <dbReference type="InParanoid" id="A0A1E5RG14"/>
    <dbReference type="OrthoDB" id="6512918at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43158">
        <property type="entry name" value="SKFA PEPTIDE EXPORT ATP-BINDING PROTEIN SKFE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43158:SF2">
        <property type="entry name" value="SKFA PEPTIDE EXPORT ATP-BINDING PROTEIN SKFE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="9"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="275" mass="31227" checksum="C06E19C9D5CD4401" modified="2017-01-18" version="1">MSFESTKAVVVDDLTFKYPNSNIPSLQKITLSVDWNTTNLIIGSNGAGKSTLLKILAGKKLCLNGNILINGVNPFSSKFRNEQEFGMNTCTYFGTEWCHQEIINRDIGVLELLDSLGYSQHFKTRGDLLISILEIDVNWRMFKLSDGLKRRVQLVMGLLKPFKVLLLDEVTVDLDVVARCKLLDFLKNETKSRQCTIIYATHIFDGLNDWPNRIVHIHKGQIVDDHKYSDIEFLDSYKSNTDAAKPTNNKLKVPKSNSLHPLALSWLLXDQKTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGE9</accession>
    <name>A0A1E5RGE9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein SOP4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1521</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the export of PMA1, possibly through the monitoring or assisting of PMA1 folding and acquisition of competence to enter vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SOP4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ85990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGE9"/>
    <dbReference type="FunCoup" id="A0A1E5RGE9">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGE9"/>
    <dbReference type="InParanoid" id="A0A1E5RGE9"/>
    <dbReference type="OrthoDB" id="3971980at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031395">
        <property type="entry name" value="Sop4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17081">
        <property type="entry name" value="SOP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184732" description="Protein SOP4" evidence="12">
        <location>
            <begin position="22"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ85990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="238" mass="27068" checksum="48BDACEFC6D02971" modified="2017-01-18" version="1">MLHSLISLLVFTLSLLHVTIASSATTDIRGKVDLRGYTKKIDRNTFGRISLTLNQIYQNNSIPESNYFYDAQTTVKTKNGDFEFENVVLDTKNEYSYYVLKAQSLDFNFQPNRVLVKVQSNDTSAVQYFANYMGREYFPSPEILYPEQLQELKTLSFRPLAEVPLRNYVQPRKLGFLKSGPLAGIFQSKWKTGALVAVIASIALPYIMEKLDPETAKAIKEEKAKMKRNKLNGGTSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGJ9</accession>
    <name>A0A1E5RGJ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="15">ATP-PFK</shortName>
            <shortName evidence="15">Phosphofructokinase</shortName>
            <ecNumber evidence="15">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g1080</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15 16">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="15">Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 15 16">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterooctamer of 4 alpha and 4 beta chains.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="15"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGJ9"/>
    <dbReference type="FunCoup" id="A0A1E5RGJ9">
        <property type="interactions" value="911"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGJ9"/>
    <dbReference type="InParanoid" id="A0A1E5RGJ9"/>
    <dbReference type="OrthoDB" id="537915at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005945">
        <property type="term" value="C:6-phosphofructokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070095">
        <property type="term" value="F:fructose-6-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055082">
        <property type="term" value="P:intracellular chemical homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000007">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000008">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="entry name" value="Phosphofructokinase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03184">
        <property type="entry name" value="Phosphofructokinase_I_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009161">
        <property type="entry name" value="6-Pfructokinase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015912">
        <property type="entry name" value="Phosphofructokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02478">
        <property type="entry name" value="6PF1K_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697:SF57">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000533">
        <property type="entry name" value="ATP_PFK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00433">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="11 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 15 16" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 15 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase" evidence="17">
        <location>
            <begin position="158"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofructokinase" evidence="17">
        <location>
            <begin position="547"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal catalytic PFK domain 1" evidence="15">
        <location>
            <begin position="1"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interdomain linker" evidence="15">
        <location>
            <begin position="533"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal regulatory PFK domain 2" evidence="15">
        <location>
            <begin position="547"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="308"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="229"/>
            <end position="230"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="259"/>
            <end position="262"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="306"/>
            <end position="308"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="350"/>
            <end position="352"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="434"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="440"/>
            <end position="443"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="617"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="674"/>
            <end position="678"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="712"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="719"/>
            <end position="721"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="779"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="805"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="811"/>
            <end position="814"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="912"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ86004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="945" mass="103972" checksum="F5A7F63834BE6379" modified="2017-01-18" version="1">MSSQIVISTSSEEEYKQLVEFYHSLSSFEAIKSVSINSKELTKQYWFTTFDLFQNEIIEIPINDKSAETFFIIQLTTSSGAATYKPTKNTTTKLFSLNEDLVSQKEFKDPLNNVYTINAAISNPEPNPDFASSSSTASLLNLAGANSNIASNGQVKQKIAVMTSGGDSPGMNAAVRAVVRTGIYYGCDMYAVYEGYKGLLEGGDLLKPISWKDVRGFLSEGGTLIGTARCMEFKERWGRKQAAKNLIERGINALVVCGGDGSLTGADLFRHEWPSLVKELVEQDQSFTPEQVEPYKNLTIVGLVGSIDNDMSGTDSTIGAYSALERIVEMVDYIDATAKSHSRAFVVEVMGRHCGWLALMAGIATGADYIFIPERPPKKDQWQAELKEVCQRHREKGRRNNTVIVAEGAIDDELNPITSEDVKKVLVEIGLDTRVTILGHVQRGGTAVAHDRWLATLQGVDAVKAVLENTPSTPSPLIGINENKIIRMPLVESVKLTKSVAENIENKHFDKAIALRDSEFIDSYDNFIATTVQDDGSQLAPEGERLNIAIVHVGAPTAALNASTRSAVLYCLTRGHKPYGISNGFSGLINDGEVKEMKWLDVENWHNLGGSEIGTNRVLPSEDLGSVAYQFQKNKFDGCIIIGGFEGFTALKELYDARKQYPIFNIPILLIPSTISNNVPGTEYSLGTDTCLNTLVNYTDSIKQSASATRRRVFVVEVQGGHSGYIASFSGLVSGAVSVYTPEKKIDLTDIQEDIALLKENFKHDNGETKSGKIVIRNEVASDVYTTQLVANIIEESSKGKFGVRTAIPGHVQQGGVPSSKDRIFASRFAVKCIKFIEEFNKKNTKAVKNVSAISSEDAHSLKFHYDKISGEKVFNVDTEDNSCCVICITGSHLTFKPIQEVWENETDLVLRKGENIHWEEMNKIGDIISGRLMLRRELEANTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGL0</accession>
    <name>A0A1E5RGL0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Arginine permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1580</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGL0"/>
    <dbReference type="InParanoid" id="A0A1E5RGL0"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000001">
        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF4">
        <property type="entry name" value="ARGININE PERMEASE CAN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="201"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="293"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="386"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="420"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="464"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="499"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="9">
        <location>
            <begin position="58"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="634" mass="70004" checksum="3C00E1E1B37BDA39" modified="2017-01-18" version="1">MNQESYHLGNMSSDKVSNYKTSDMLKSTLSTSSSSHISSTEQGDVVETKVQRGLKSRHISMIALGGTIGTGLFISTATPLQQAGPFGALLSMSFLGLVAFSVTQSLGEMTCLIPVTSSFTVFTKRFLSPALGSAVGYSYAFSFCITLALEISVVGSIVNFWTDKVSIAAWNIIIWVIVVFFNMMPVGIYGEVEFWCASIKVISIFGFLIFGLCMVCGAKEVNGFKHIGFHYWNDPGAFGTGIISKNINEARFLGFVNALVSAAFSLQGIEVCAISAGEAQNPSKTIKKAINKAFLRIILFYVGSLFFIGMLVPYNDPKYSSGDSFISASPFLLAIENCKVKVLPHIFNAVILSTVISAANSDIYIGSRVFYSLATQGQAPKILKKTTKLGTPIFAVALTSLFGFLSYLQCSEGGQTAFNWLLNCTTCLGATAWIFTSFCHTRFRKALKARNISLSDLPFKAKLVGWRSMGPWAAGCVMTFIIFIQGFPSFCPFDYQTFLADYISVIFFFVLWGGFHFYNCLVFTPKQDNLQNENTESKTIAQKLRNVFRMKNKVSFKWQSLFIPLDEVDIDSDRIDPDDLIWEENGTESQQKLNEADEDINFVTIYSNASRAPKGQRFAIARHGLWESICNLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGL4</accession>
    <name>A0A1E5RGL4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">D-arabinose 1-dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1360</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGL4">
        <property type="interactions" value="218"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGL4"/>
    <dbReference type="InParanoid" id="A0A1E5RGL4"/>
    <dbReference type="OrthoDB" id="5286008at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045290">
        <property type="term" value="F:D-arabinose 1-dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070485">
        <property type="term" value="P:dehydro-D-arabinono-1,4-lactone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42686">
        <property type="entry name" value="GH17980P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42686:SF1">
        <property type="entry name" value="GH17980P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="14"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="334" mass="38306" checksum="644974037110FDBA" modified="2017-01-18" version="1">MAKEDKSLTGIKPLVLGGAVFNYQYNADPQNMDIVNILKXGIESGLVNCIDTSPYYGPSETLLGESIAKLQETYNLWTNRDEYYVITKCGRLKLDDFDYSREWIRKSVLRSIERLKCGYLDCVYLHDIEFNNEVEIMEALKELHKLKEEDKLILNFGISGYPIDFLHKIAVECLKNNEIGALDCILSYCNGCLQNNMLFKKSYKMINEGHLKMLSNASILSMSLLCGQKIKEFHPCSLELKHVFDTQVKTYCDQEGINIAELSTKYAIAEFHDMGPTVVGVSNVFELQTAIDTFRSLQKQDMQLNETELKHVEYIQNEILGKKFMNMTWXSGKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGN5</accession>
    <name>A0A1E5RGN5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Putative ferric reductase transmembrane component 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1099</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGN5">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RGN5"/>
    <dbReference type="OrthoDB" id="10006946at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000293">
        <property type="term" value="F:ferric-chelate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033215">
        <property type="term" value="P:reductive iron assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050369">
        <property type="entry name" value="RBOH/FRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972:SF178">
        <property type="entry name" value="FERRIC REDUCTASE TRANSMEMBRANE COMPONENT 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972">
        <property type="entry name" value="NADPH OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00463">
        <property type="entry name" value="AIM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="258"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="451"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Ferric oxidoreductase" evidence="12">
        <location>
            <begin position="161"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="528"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="594"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="533"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="598"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="610"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ86068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="759" mass="86905" checksum="4CA534C1FBA94196" modified="2017-01-18" version="1">MSAQNIQTLMAPPESPEAGSYKRVFFDLIRLPDFQHKLEWEFSHSLIAKSANXTFXLALFIICGLIPLANYLSLKFPKINMFFSHYHKHNRNLYQRRRSSNTNLDPAANTFFHKWHKFINKKISSVIYNTSLKQFLLWGTIVSVCSTWRNYGDLINITKRLGRISCALYPPLLCLTLRPSPLPHTMYLNLIPVHKWISRIVILTSFLHTVFYFSYFIYFNSLFKVLKWANFTGVLAMCCFIIIGVTSLRLVRRGNFTFFYSNHYICTWISVVLLDIHSRPTIKWFTAMNYILLVGQIFYRLYHTKKCDKVSIVNISSNLSIMEFDKKYMTKQPEYPSSHVRINNLYHESNHVLQNWCKLIWYTVIPLQHPFTVASLPNEDNVRLIIRKXNFPLIDQAPYAITGSFESKVPFLHKPNKAPHYIASSLTSPVSPLLFDESHGKKKFYIDARKVLIVVGGSAISFGLPLLRVLNYNGINCKLIWVTRDYQDLCLLNYFQKNFYGLEIYITGEDFKNEQDLGIDYVDYEDEDDEENLTNSVNNQQSSNPANTGYGSISRLKKHLDSKPNIPNTPQNQPVSATITTDSADDEIDFTNFHTNKQVKDKKSDKKLGSHNSTLPEQKSNFRKPSVVFPPSVDTSYGQETRIQVPSGVCVNFGRPTLGAKEYVWCLEGDCDVSMIQAALFEEGFCVEGTLEQEDLRADEHSTLNEGNATGSSSAGKGGITPEKLSKTWVVAAGPQGLVDSAKKWSLEHGFQFHDEVYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGP5</accession>
    <name>A0A1E5RGP5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Origin recognition complex subunit 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1569</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ORC5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGP5"/>
    <dbReference type="FunCoup" id="A0A1E5RGP5">
        <property type="interactions" value="888"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGP5"/>
    <dbReference type="InParanoid" id="A0A1E5RGP5"/>
    <dbReference type="OrthoDB" id="365981at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020796">
        <property type="entry name" value="ORC5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047088">
        <property type="entry name" value="ORC5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048866">
        <property type="entry name" value="ORC5_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12705">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12705:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14630">
        <property type="entry name" value="ORC5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21639">
        <property type="entry name" value="ORC5_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orc1-like AAA ATPase" evidence="8">
        <location>
            <begin position="8"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="ORC5 lid" evidence="10">
        <location>
            <begin position="239"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Origin recognition complex subunit 5 C-terminal" evidence="9">
        <location>
            <begin position="338"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="298"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ86078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="501" mass="58515" checksum="8E94C429EE6B0412" modified="2017-01-18" version="1">MTIAPSSVVARDYQLQVLDNFIDSECLPDLFPNNVVVQGFKSSGKSYCVREYFKSLNQQGNFKVFVTRYELFSWKNLLTSIARISYFALRSAFPNVETTYDIDPASVEDFYLLVEFFTKLFQIYDAQLEGPKSFYVIIDNLDSLPQIDFLVFARLVKTHESMPFDLKFKLKFLYTIENVSFYDKYRTFDLPLVVFPRYTQEEILKILELHHQDFANSKELKHLSLEKGSAFDKKYFCKTYLKFIVESFYSYTGSDVDSLKDIAILKWPLYLQSVTTENISNAAELYKKNIHLWTNTGDSLENNTSTEENTNEGDSGLEPQQKKPKRNKLNGLVEQYALSNLTKYVLISVYLASFLDQKFDSKNFSRKFHFKKGKGAYGSRVKQKLQPETMKPYPSTFERLLAILQAVFTFNRRTEEFLESAEEEQSMRNEYFAHTNAMKSNVEVYESFSELVSLKLVLPSKVIYPDLLDSKTKWKANLPWETMLQIAKSVDFDIEDYFEFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH48</accession>
    <name>A0A1E5RH48_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein transport protein SEC39</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1204</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH48">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RH48"/>
    <dbReference type="OrthoDB" id="342024at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013244">
        <property type="entry name" value="Sec39_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40787:SF3">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40787">
        <property type="entry name" value="SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08314">
        <property type="entry name" value="Sec39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Sec39" evidence="5">
        <location>
            <begin position="358"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="6">
        <location>
            <position position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ86232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="650" mass="75261" checksum="4A2E4905EBEDF670" modified="2017-01-18" version="1">MSDPVSLLTLADCVSKGDVESTKKLLFTIPHINTRFCKCIAVLWPEYSAPDSLKFMGTYYGKLDLDSTTESQLIQASTAEEIELDSYLDSQLRLFLGFQNSXAMDFSTVDHFFRARVLVVNMALPNDPLYCLPLAKSLNISNEPWITGILKPFYYFKKTKTLENLNLVKNFQEAAPEDIISEMFEHFLKTSGEVKMHIMNRQIKPYIDYMQLTDLFIEKYLKSNDFHMDYSTAFFLLQHPITDGQTLNEKYYQTLIERIWNTSDDFSISELRSCVQSLPENTVIQTLLGTLGKKTLLEYLNVMEELKFNNFQKIVDLSKNANNIQPVYFTFLCDQYFAASLKKTSSSTCLPWFFESPLFDSLDTKKKHLLITESLIKNNKLEELKTFSQEQEIDHDFLLNHFWKLFHKASNGLPKNSNMKQCYSLLSMLNDEKLTLLLDLVQYLAVEFPSFQLVPNQPFKPSHILQLSNSPLLLIDKLFNANENQYLHPEETVQLLKKLFTVLDVVNIDDAHLQSKVLASSIEIALVKNNFNFAYSLTIQLLENKNACNYWFVILQVGKFIDADHWPDLETPTEIIFLQMEILSKLLIVIPPDDMESAVSEYSRLELELSTRNLINDEYSLENKQRFNNTKSYENFLSSFKSNVSNLLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHB2</accession>
    <name>A0A1E5RHB2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA damage checkpoint protein LCD1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1600</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHB2"/>
    <dbReference type="FunCoup" id="A0A1E5RHB2">
        <property type="interactions" value="178"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHB2"/>
    <dbReference type="OrthoDB" id="4078000at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018622">
        <property type="entry name" value="DNA_damage_chkpnt_Lcd1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09798">
        <property type="entry name" value="LCD1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="178"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="89"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="178"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="208"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ86256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="835" mass="95547" checksum="A5F24E310C0CE67E" modified="2017-01-18" version="1">MSLDNTIASDSDDDDLLLELAKYTQHTSKRNDVNGLSLSSNTNTPQGHASSDNANHSTTLVSFTHSKSSTPRTPLKQAPQNNAAHDLQLLLAKGEIAVLRSKLKALEVEREQDRKLFNEEKDTNQQKYAKDLEDVKYDLNKLEDEKQFLKLQLKTTARMTNVHNKSVDQNATMITSPNTILQNETIPNDNASSETEHSVNHKRLKQPKHAESKPSLGSDKKLENLPTKTKIKLHFRPKTHTDENIVLLNRVLKNKIPGLLHPCIYYLDRKSGHTEFHDYAMNIVSEYGLNKFIESILDYWLQKVNNILNTENKEGELPSDHTRYIPFILSIVSEIVKFRPSACDVDILVNLFIVIKKMISTFEYVFKSTTIDQKQNSKYLQKYAVHSRTSITKNSKQGQESWEMLYPESEYTSCSNGMSPGFTQDILAGDDSLFEIPTFHTEFVDIIIVIYSFDVLELISKNLSFQVQNKIKHEGNINTVDDTLVTKIMSLFDDLTYLYKLSNTISFQPIFHPIYNVSVIMESFLQISHLFLEHFQAGKLNVMEQTIYAKWQYLITRSSEWLIHLNLNNVLKTLPAKYNIHSLLRSIGSPYDAKNSNLEFGNLITSLETIMEDKVIYQPDPNAVTYIYFWNLFLTKQILSNISKYLFMMSTSDLLNFKSDEIKEILNNVIKLLSVQYPFLLKQTGEDFSLLQDIIVRCLRIIQHCHNMSLNTSSQDVPLRLENHSKNSKPWIENSAMKAAQRSTTNGSTHSASDRPIFDSVLNDEKYKLELIVTFSRIVFQNKDGEDVFVKKDKSVSNGNTSALIIKEICRDMLDTLITPDESDVIFDALTPTAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHG8</accession>
    <name>A0A1E5RHG8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1153</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHG8"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="unsure residue" description="D or N" evidence="1">
        <location>
            <position position="5"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ86023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="109" mass="12309" checksum="1E9EC4AC6E9A6F50" modified="2017-01-18" version="1">MTQTDADANXDRVELLRNXIIDELXKQYEKQVTDLQLAFNNNLDQLSLTKEXLGSKYEKFXHPVDAGSSITTSKTKTKIQSCLPSNEVXLNIGEVLRRESGDALEKEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHI8</accession>
    <name>A0A1E5RHI8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein BNI1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1582</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the formin homology family. BNI1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHI8">
        <property type="interactions" value="521"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHI8"/>
    <dbReference type="InParanoid" id="A0A1E5RHI8"/>
    <dbReference type="OrthoDB" id="1104827at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005522">
        <property type="term" value="F:profilin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045010">
        <property type="term" value="P:actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070649">
        <property type="term" value="P:formin-nucleated actin cable assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2220:FF:000006">
        <property type="entry name" value="Cytokinesis protein sepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000898">
        <property type="entry name" value="Formin BNI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.30.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.150">
        <property type="entry name" value="Formin, FH3 diaphanous domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051661">
        <property type="entry name" value="Actin_filament_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010472">
        <property type="entry name" value="FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014768">
        <property type="entry name" value="GBD/FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010473">
        <property type="entry name" value="GTPase-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47102">
        <property type="entry name" value="PROTEIN BNI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47102:SF2">
        <property type="entry name" value="PROTEIN BNI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06367">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06371">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01139">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01140">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51232">
        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBD/FH3" evidence="5">
        <location>
            <begin position="212"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="domain" description="FH2" evidence="6">
        <location>
            <begin position="1436"/>
            <end position="1854"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="64"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="271"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="970"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1105"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1210"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1318"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1378"/>
            <end position="1444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1850"/>
            <end position="2124"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="790"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="95"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="111"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="135"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="152"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="278"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="980"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1113"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1123"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1217"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1318"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1333"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1383"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1418"/>
            <end position="1436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1873"/>
            <end position="1882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1883"/>
            <end position="1893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1895"/>
            <end position="1910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1921"/>
            <end position="1933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1995"/>
            <end position="2018"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="2019"/>
            <end position="2028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="2064"/>
            <end position="2080"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="2095"/>
            <end position="2113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00037935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="2124" mass="237067" checksum="0A50864F30A8A614" modified="2017-01-18" version="1">MSRNTSSMGKHERGPSHGKTINATGNSGNSSGGSGRLLSNLKKLGSSSAKNQNLSTLQISEPMDPVHLQPPIAFQSSSDNHSFGPMSPRLKHQNSETSLQSSSSNQQPLSKTTTYASVNNGTLSTLEGRSARAQYESQMTSLQSSPNSLAHSPSRNTNSFSSRRSSASNEFNELATQYTQQTSAAAQSIYSYSMGTSNLHRFMGPDGKIKIEKPADNREVQEMFDDLMEKRDFHSLPSHAQQQLQSYSVDKKWLLVQQDLQAELRKLNKKYKDKNTGVKHSQNPSTLGPSTSSLYTHKKSYSNQNNNVSLAPLTTQQASSSSNDINYLPKNSYTQNSMGTAGQRKMTSQSVPSPNKLHPAHSNHIINGNQSIYKSNTRNISSGTLHSPEPSQLSPAYYVRRLISDQLTNSQLNDLWISLRTQPLDWVLAFLDAQGHVAISNVLLKFYKLNYTTTPTSATLRAESLSSDQLDTESLLFKCLKTILNLREGANECAGSKLLMNAIAEGLLSLRIATRRSATEMLTFIMAWQNGKHYSNVLGALDQESPVSSNVHIQARLISPNKRKSFLMDNLRAQMQISDNHKMKRIEEWLLVLEYTLDGRGAMGSMVGASDEIKNGGENALLEYCYLTMFMINKLCNTPSNVHQRTLLRSRFKTYGFGRIMQKLELLNYEKLNNELRIFEDKTADDYTELISDSNLTEEVDMENPVELLSTMWSNYKDTDAGKHLISLMQHVFLTTAKNRKNSDLSDMNKTLKLIDGLVSNATMSSLDVESSFNMAIQRLYDSMQTDEVARRAILETRELTRKYEEIKAENDNLTEKLEKAEDGLVGKLQRDINERDRVLEKNQRVTESLQKELDYLKRQMILEKHNHEVEKKNLISSGNTPVLPFEETFATPNPINPDRIKAIEAVLQRKPGKLSRLDPGHKPVSTHPSVAMALSKSATPPKEPNARLKALRSRMEDIEKEARILEGTNFSDYDRPPVTKTNYQSKPQDGSPEKIAIVEKLDKLKNMLSHIQNESNDISKFNADSRVNDLLKRQKGVALKLLMDLEKKFGSTLDLDSQTKNMVDSLMTHSSDDSTLDPVALQLKLDGLNQLLLELNKKVTFLEQPSPRATTSSVSSSSSESSSEDEEEEEKEGNSGSRIPDTAFLSNLSEKYAYLSAETGKNTSTMNVRGSQYYRKAFMQRLKKSNTGHLLSQDTSQPSAALNDYAKTNENESPESDYNTSQLSGHDNATIASDSVDTETLHTINSKAGETSTIRSIATTERSNNDASMVETATGLSLPPPPPPPVPVFTPNGIQKPLVGPTTKQGVSKLDLSNFSHNEQQNPLNVSGSAVLPPPPPPPPIFTPNGIKVSPGTLLQPPASPTGLFVIPSAEAENAALTPKGAVPPPPPPPPPPPGFGSPVGGGPPPPPPPFMMKPNTMFNSPVPDSNDGNGSSLFQKYPRPKRKMKQLHWEKIDDAEDSFWKNQKPKKYADDLYGKGALAKLESAFAAKEIKKLASRRKEDLDKITFLSRDVSQQFGINLHAFASLSTTELVKKVLNCDKEFLNSVSVIEFLSKSEICTVSVNLARNYAPYMTDWESVSKVEEAKPPEKDITELQRPDQVYVSLMVNLQSYWFSRMRAITLLTSFEKEYSDLVVKLRKIDRAVDSIKNSDNLKGVFDVILAVGNFMNDSSKQAKGFKLSTLQRLTFIKDEKNKLTFLNYVEIIVRENYPELLDFVKDLEPVLEVVKISIEQLDHDCTEYCNFVKNVTQSIEIGNLSDSSKFHPWDRVLQKVLPVLPEAKKKAELIHNEIKLSIMEFETLMRTFGEDVTDRFAKNSFFSKFADFVNEFKKCQQYNIKLEEEERAYEKRKKLVEEQQKHQAAKQKQATDKSESQENETADDAAGDDRDVMDKLLNKLKTTTTNKSDSASARVRARARKKLSDMNSHSRSPSNILDNFKDEASLTKSGGSIVYSPESHKITPSDAVRQSPTPANASKIALGTSDEEESGDISNRAKSLLDEIRGNQERDGRRHSSSDTERRKLRQMHRKSRSDAASNKLRFYHNSADDLHEVSESDVAQLQEQSEGDNRENDDLSSVNRDEQTAEEDDPTVLGELAKLSEKPIEETGSKALKQNDEFFDAVDSSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHS1</accession>
    <name>A0A1E5RHS1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chromatin structure-remodeling complex subunit RSC7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHS1">
        <property type="interactions" value="351"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHS1"/>
    <dbReference type="InParanoid" id="A0A1E5RHS1"/>
    <dbReference type="OrthoDB" id="5598844at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013933">
        <property type="entry name" value="CRC_Rsc7/Swp82"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08624">
        <property type="entry name" value="CRC_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="479" mass="54850" checksum="1343FAD6E1EC0E10" modified="2017-01-18" version="1">MNTNAETEVNEGNQTFNEDLEESHDTXDHKVEDQEQYEKEQAHLHDDADEEYHENDEEEEQNLEDQANATPKDXXPTATHDXAQPRRRRRGRPPLKEKKRGLMISISSEPDLKMKYNQDEVPVDENGNIVDLLNDEYVLPKDLEGETKVNAFGELQGGRNYRVQTFTLPGRNQGKTLYVLGNDMARLLKYKDAYVFFLNYKTTLYKTFITDQEKEYLLAQGVLKPGIKSRTISVTTAKSCFREFGCKIVKHGRPGIDDYYVTTEAYPPADHPVSAKEVAKKFLSNSRGVHSLNSASATPISASSFSPSLTSGNFAPKQRVTPNTSNLMPSDYNARRTVEYFNMISSNMNISLNDKINKENWIFMHAQSCSGYNEDLFYEREKIQFIDNSGVRDPYTNTLHVPLFTQPKKILKKTLSPLTTQDKQKMLKKKIIYETRIKDDNLKLVKTGLSDLPLDLFKGFLDENVCKEIDKQVSYESKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHV3</accession>
    <name>A0A1E5RHV3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sporulation-specific protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1642</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHV3"/>
    <dbReference type="FunCoup" id="A0A1E5RHV3">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHV3"/>
    <dbReference type="InParanoid" id="A0A1E5RHV3"/>
    <dbReference type="OrthoDB" id="536881at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051648">
        <property type="entry name" value="CWI-Assembly_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31018:SF12">
        <property type="entry name" value="SPORULATION-SPECIFIC PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31018">
        <property type="entry name" value="SPORULATION-SPECIFIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="403"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="415"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ86482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="458" mass="50345" checksum="92B7EB4EA92C92F6" modified="2017-01-18" version="1">MQMNFNTSIKLIVLAYFVGFIYGTVVPSNKIYANNSIPQDKETTALPEICSLKKHEVSDAYQLQGLQMQCSTLSGDLVFQNYIDPMINLGNIKIIQGSLTVEESSSLVRIEAPQLQEVGHDFKLKRLTSLTSIYMENLSTVDTILWKVLPILSTAQLGPSLKKTTKIIISDTSLTSFDGCHNLKNLSILNLNNNRFMEEITCNVESVSEQLSIAANARDVEISLKELQWANNITIRESAKIELPKLESVKNSMEFIDNYFSTLKIPNLETIGGTLGLLENRNLKTIDINNLTDVSGGIMISKNSQLKSINFFSKLQQIGGAIQFDGTIDKVEFPKLKLVKGSVSIISDSDDLDCSQWTSPKNGVSIIRGGKITCSSLKKETNISLNENGEILDSDEVEKVKDDVEGRDSKSLQPSNSASSKKANKTSSKSKTANAALSNTPFSSWSLGLILTIPLLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHZ4</accession>
    <name>A0A1E5RHZ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcription factor MBP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1287</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds to MCB elements (Mlu I cell cycle box) found in the promoter of most DNA synthesis genes. Transcriptional activation by MBF has an important role in the transition from G1 to S phase. It may have a dual role in that it behaves as an activator of transcription at the G1-S boundary and as a repressor during other stages of the cell cycle.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHZ4">
        <property type="interactions" value="789"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHZ4"/>
    <dbReference type="InParanoid" id="A0A1E5RHZ4"/>
    <dbReference type="OrthoDB" id="3973086at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030907">
        <property type="term" value="C:MBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033309">
        <property type="term" value="C:SBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.260.10:FF:000004">
        <property type="entry name" value="Transcription factor MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.10">
        <property type="entry name" value="Transcription regulator HTH, APSES-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036887">
        <property type="entry name" value="HTH_APSES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018004">
        <property type="entry name" value="KilA/APSES_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051642">
        <property type="entry name" value="SWI6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003163">
        <property type="entry name" value="Tscrpt_reg_HTH_APSES-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828">
        <property type="entry name" value="ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828:SF15">
        <property type="entry name" value="TRANSCRIPTION FACTOR MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04383">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01252">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54616">
        <property type="entry name" value="DNA-binding domain of Mlu1-box binding protein MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51299">
        <property type="entry name" value="HTH_APSES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HTH APSES-type" evidence="8">
        <location>
            <begin position="5"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="731"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="110"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="243"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="768"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="115"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="152"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="183"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="247"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="278"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="9">
        <location>
            <position position="851"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00073969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1026" mass="114251" checksum="CDF6D634E14E97C6" modified="2017-01-18" version="1">MSDQIYSAKYSGVDVYELVHPTGSIMKRKKDNWXNATHILKAANFAKAKRTRILERDVIAHQHEKVQGGFGKYQGTWIPLHLAFQLASKYDVVEELRPLFEFVQLPGAEAPPPAPKHHHASTKNGSAKKAKASTTTKPVSNFNMIAKPXPVPNTNNNYTKNNGSKTPSTDSLNSVDGVLPASQYESRNGSITTSSLAERNSRGNMTSSGNVQSFNFIASPNSTITAPPSTLTSAYQINPSMATPMDSITSTGTITASGKKRGRPPLHKAGNLKKPVTLQRSKSDFQAISSSKLQTESNSQSTTQAIQLPPIVSKPSGSITLTLSQQQQHQQVQPHTSPLPLLPSTHQGSSFSWQNPSFPLTSSISQFSNGTINLNNSLHSIHGGTNNNNNAVYINSANNNHNIPGSQPISRMNSFSMMSMIENGQPNSAIRPSLTSKKNFSHEQNVFTDMSSNAKNIHDFEYSTHDGNLDIKAQQHSGAALHTGNPRSQDVNNLHAMNGEYYSMNAHNEIADQMEPLRSGSEMWKNSRRRSSTEDDTMDLLGSGNENIAENHNNKKLQTLSTFHLNNAYLNEMASHLIYNTAMPEFLKQPNNKDLNYEVXNSPIDSEGNTVFHWACAMGNVGMVEKLLSNSEVNIYLMNHAGENGLLRSVLFHNSYTNSTDCFQXLLDLIGNELITSRNNNGRNILHYIVTKKSDSPSAVFYMGKTLEFLSSSPLNKNKMKLLINQQDYKNGDTPLHIASSNQDDFFYKTLLNKGCDVNIKNLKNKSPVYESGIVPPPPTSDFPNGEKDEDSEATDSNATFTGEQPXITKSVEQNSVQKSKTVAFIYDTVLPELNKEVNQLSYMYYKNLKDKLSRNNELHKIIEGIDKNITXIERKFVAEAINREGFDRAMHGQQIEDELLNLDTKHNGLRRSLCRARKSMLQVLEKQESQFFTKRIGQLRDFDTTSGKTSIISKQDLLRKSILVTLKQLKRKKILTKTTDLYTDDKIQXKFFKYRKMISKGSDLDISEIDNCLDEILKTIGGDAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHZ5</accession>
    <name>A0A1E5RHZ5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Vacuole morphology and inheritance protein 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1280</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VAC14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHZ5"/>
    <dbReference type="FunCoup" id="A0A1E5RHZ5">
        <property type="interactions" value="911"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHZ5"/>
    <dbReference type="InParanoid" id="A0A1E5RHZ5"/>
    <dbReference type="OrthoDB" id="5574975at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070772">
        <property type="term" value="C:PAS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006661">
        <property type="term" value="P:phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026825">
        <property type="entry name" value="Vac14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021841">
        <property type="entry name" value="VAC14_Fig4p-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16023:SF0">
        <property type="entry name" value="PROTEIN VAC14 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16023">
        <property type="entry name" value="TAX1 BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12755">
        <property type="entry name" value="Vac14_Fab1_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11916">
        <property type="entry name" value="Vac14_Fig4_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="5">
        <location>
            <begin position="88"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar protein 14 C-terminal Fig4-binding" evidence="7">
        <location>
            <begin position="636"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="334"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="367"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="944"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="338"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="979" mass="110011" checksum="CBCDCCDADD6256E3" modified="2017-01-18" version="1">MEREILKGLCDKVYEKRKAAALVLEKQIKNLANANDFQKINAIVDELCDEYFPLSVEKAVARNAKLMGLATTMIALGPKNIPSYLSTIIPPILICFEDQNEQVRFYACESLYNIAKIAKGEILVYYNDIFDVLCKITSDPVSNVKNAASVLDKLIKDIVGEFGSRHETVVKNDGMNDLPPATKINSVTGEVLQERYEQDTKKLSFSLPKFIPLLQERIYTNNPNTRMFIVSWIQVIASCPDLELITYLPAFLGGLFAYLSDSHRDVRVVTSNLLDLFLQEIKRVSELKKILAMDAASTHSLSINVNNTLTSEANTVTGNLKNTNQTLLSTKQGFMRLSSDDGSSKKLTDGPSITERKKTLINALDQWSGNANNSDDSNDDSGTNVHRYNSKKENGSSDSTQRASEDLEEHEQFHEQFHDQSNVQRDLNSSAPTIQTSRFGQDYVPGQNVVLDFPKFIQVLITSLNSSEPKVQVVVLKWLEAILTLSPRGFVSTLSNILPLLLKHLSDTNPKISELATSINYKLIDVAGKLDEGEKINYSSMINNLSLNFKEGTATTKIACLDWLVFIYKRISATSMMEFNDGIFYILLQSMSDQDNRVISKSLTLLSCICANGKNDSYFSNFVFKVLDLLKKDIDILKTRASFILRQLCLQLGAERIYKITSLTLDKWDKSDMTFIQLVIQIMSTDLITAPDLKALRKKLKRNEDWTFFSIIFKCWCYNSSSLLYLCLISENYELAYNLLQILVEQDAIDSSTLIQIDVLVQYLESPVFASLRMQLLEHQKYPYLYKCLFALLMILPQSKAYGILNNRLSSVMKLVSVTTALPTSPSSGGSSSPMLKTEHNVSTRSLSINSTSSRSITQNSFQFNDLLEYFRNVTISLDDECADGNSDKVMPMIRTNSNKQESAKHVSKQSVGNRISSMSKVYEPMAPVTPQMANGMFTDAMPPILPRKLSSSTYAERQSSEAQHEEDDNDSVVFNPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI54</accession>
    <name>A0A1E5RI54_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">L17</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">RP18</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">YL16</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1189</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI54"/>
    <dbReference type="FunCoup" id="A0A1E5RI54">
        <property type="interactions" value="1226"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI54"/>
    <dbReference type="InParanoid" id="A0A1E5RI54"/>
    <dbReference type="OrthoDB" id="2436667at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13156">
        <property type="entry name" value="KOW_RPL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000014">
        <property type="entry name" value="60S ribosomal protein L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000915">
        <property type="entry name" value="60S_ribosomal_eL6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041997">
        <property type="entry name" value="Ribosomal_eL6_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10715">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10715:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01159">
        <property type="entry name" value="Ribosomal_L6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 7" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00075813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00080112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00082776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="174" mass="19853" checksum="1D82950FA92BF4EE" modified="2017-01-18" version="1">MSAQQWYPSEDVKVAKKTRKTNRPQKLRASLVPGTVLILLAGRFRGKRVVYLKHLEDNTLLVSGPFKVNGVPLRRVNARYVIATSTNVSLEGVNVEKFNVEYFAREKLTRKQKAEANFFNEDQPKKEVKAERVEDQKIVDKALLAQIKQTPLLKQYLGASFSLKSGDKPHQLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RID7</accession>
    <name>A0A1E5RID7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Serine/threonine-protein kinase SCH9</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g1165</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RID7"/>
    <dbReference type="STRING" id="56408.A0A1E5RID7"/>
    <dbReference type="InParanoid" id="A0A1E5RID7"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000116">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="200"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="14">
        <location>
            <begin position="460"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="501"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="631"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="16"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="172"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="506"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="546"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="686"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00074478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ86635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="702" mass="78539" checksum="73D99123E5343AF9" modified="2017-01-18" version="1">MNYGYYPPQGFGTIDNNTTTTTTTTNNNSNNNNSNNNSINNSINTTNTTNSYNNVPSNVQNNKFIGNPTIALSNPTNTLPVSGYITTATAQGYTLASGINDTTLNGSNTANNTGNNNNNTTTTTSNGYNTAYNSINNNINNNSNSINSINNNSSSQLTSNLTSNLNYLNNTINKNNTNNANNHGSHKNTKFRQKYGPQDFEVLKLLGKGTFGHVYQVRKKTSHKVYAMKVLSKNVIVKKNEIAHTIGERNILVRTASKSCPFIVGLKFSFQTVYDLYLVTDFMNGGELFWHLQKEGRFAEPRAKFYIAELVMALEYLHDIDIVYRDLKPENILLDSHGHIALCDFGLSKADLKDRTNTFCGTTEYLAPELLLDETGYTKMVDFWSLGVLIFEMCCGWSPFFAEDNQKMYQKIAFGKVKFPRDVLSPEGRSFVKGLLNRNPRHRLGAIDDGRELRAHPFLSDVNWELLRQKKIQPPFKPMLQNELDTSNFDPQFTSHQINHSLFSDSTSPHNTHQTHPTTTSNSNNNNNNNNNNNNNNNNNGNGNNPALSQGTAHGTQHQDAQIQKSHHHKQNTPLSSTLQMQFQGFTYVDQSALEHMVHGTGSSIPNSYSMSLNKRKMLQNSYFMEPGSFIPGDPSLPPDENVIDDFDDSRNKNRNGGGEGDNLNNLNEHLQMDPNKANTMGMDMGMDELDDDDEHMGFQFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RMZ8</accession>
    <name>A0A1E5RMZ8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Vacuolar basic amino acid transporter 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g634</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RMZ8"/>
    <dbReference type="FunCoup" id="A0A1E5RMZ8">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RMZ8"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17502">
        <property type="entry name" value="MFS_Azr1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000373">
        <property type="entry name" value="Vacuolar basic amino acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF198">
        <property type="entry name" value="AZOLE RESISTANCE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00173">
        <property type="entry name" value="EDTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="216"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="385"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="424"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="452"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="484"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="524"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="595"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="94"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ88255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="620" mass="67600" checksum="0BBE06B9A499FFA2" modified="2017-01-18" version="1">MSSLTEKDSASLNRNSDSLTSGSNDHGSKLNIHVSNSTGGVEHGTELVPDTEKLSHEESLVDQSTTELEGKAASKKELDTSIYEYYKGKRLTLVTLSLMLVLFLASLDIIITATIIEKVAERFGSYGKTGWLVTGYALPTGVMVLIISQIAMSLGVKFTLLVCVLIFEIGSLISGLANSMNMLIAGRVIAGIGGAGIQNLVFIIVSYIVEEKNRGLIISFVGIAFSIASIAGPFLGGAFTTMHHQPNWRMCFYINLPIGGVAFIIFYFLFNHKGSEFKYDIKKFNPRIFGHASFYKKIFIGALFQYDFIEFAICTTGLVLFLVGLTYGGDGTYHWHSAPVIVMIVIGGLLVITAFLWDCFLFDKFCAKFNQKGTPLINILVFKKFGLVAVNLVNFLMSIAYITQMLFIIQFFQFVYGNSAWKAAVHLLSTLIAVTITVVACGVFIKKTGQIKLTVITATAIAVIGAGLLQILGPHSNNSAKIGLLILPGIGFGGIVQSTMIGSQVFADKTKPDFKFQITSITSFYSFAKLIAMAFGSIIANVIFDVSVFDKVQNSQTLTSLKSMDVNELIVYRLSHYDGTDSALGKIFNDSTHNVFWLSLGLSAFSFICSLGMSNKKTDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN04</accession>
    <name>A0A1E5RN04_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hydrophilin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g628</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN04"/>
    <dbReference type="STRING" id="56408.A0A1E5RN04"/>
    <dbReference type="InParanoid" id="A0A1E5RN04"/>
    <dbReference type="OrthoDB" id="4094978at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184809" evidence="2">
        <location>
            <begin position="20"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="191" mass="21120" checksum="9C2BDF27C2A53D2F" modified="2017-01-18" version="1">MKFLYVLPILSLASSIVYGEEQEERKVEETEVSTEIVDATSTSHSTHSTHKYGKFNHTSRSHAPKNTGTHKYGKFNHTSRSHAPKNTGTHKYGKFNHTSRSHAPKNTGTHKYGKFNHTSRSHAPKNTGTHKYGKFNHTSRSHAPKNTGTHKYGKFNHTHHKRDDADSTNGAATMGAAMGLSGVLVGAMMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN29</accession>
    <name>A0A1E5RN29_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Dihydrosphingosine 1-phosphate phosphatase LCB3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g974</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the type 2 lipid phosphate phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN29"/>
    <dbReference type="FunCoup" id="A0A1E5RN29">
        <property type="interactions" value="371"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN29"/>
    <dbReference type="InParanoid" id="A0A1E5RN29"/>
    <dbReference type="OrthoDB" id="301434at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03388">
        <property type="entry name" value="PAP2_SPPase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF28">
        <property type="entry name" value="DIHYDROSPHINGOSINE 1-PHOSPHATE PHOSPHATASE LCB3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="316"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="9">
        <location>
            <begin position="111"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ88291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="424" mass="48345" checksum="F330A2ADF53301EB" modified="2017-01-18" version="1">MGTTRRGKGGNVKPQELIQEAKKSNAKHQYADPGNHAADHYKPHMSYVRFKIREFLVPFVVEETPVLFRFQQFCRTKFLDEYFIHTANLGSHTFYVLCLPMSRWLCFSKTARDLVFLLGYSIYASGYVKDFCCLPRPRSPPLQRITHSPYTTKEYGAPSSHTANATAVTLFMIYYIFYEVKGEMTWTQSIGAMLLLFLYYATLTVGRVYSGMHGLFDIESGIVIGAAVFVVRLWVRDYMETLLLHWWYPVCNIALGLFLLFAHVNPVDECPCFKDSVAFVGVVGGLEFADWLYPYVFQTANYELNFPDANSGLTHLSVWTRLLIRVFIGVACVVLWKEGIAAMLASKLGLGGTVNVEKTENRENKINKQNSIKKTTEVQKHDLEIPLHRGVSASYILWKYFVYAMISFVSVIVAPMVFKLCGVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN31</accession>
    <name>A0A1E5RN31_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Fork-head transcriptional regulator 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g796</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="OEJ88296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="56"/>
            <end position="121"/>
        </location>
    </feature>
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            <end position="380"/>
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    <feature type="DNA-binding region" description="Fork-head" evidence="6">
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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            <begin position="403"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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    </feature>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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    <feature type="region of interest" description="Disordered" evidence="7">
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    <feature type="region of interest" description="Disordered" evidence="7">
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        <source>
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        </source>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="967" mass="105401" checksum="F75A1E2A8CC008F9" modified="2017-01-18" version="1">MLAELIENVTAVLGEPVKRTTVAEQYANSNNYEREVQAYAKIAGKDWTYYVKTLNVSIGRNTTPASSTGEAPDNKDDVNIDLGPAKVVSRRHASIDYNMSLGIWELNIEGRNGAKVNFSRVQAGPGSPSIKLVSGTIIDIGGTQMMFILPDSPPTLTPNSIKHIQNKISPDHILYKLGLPRPPIHEQMHGNNPSLSSINFSGNGTVPVQNADARTMGTLYNQYGQKNGNDASKQSLAAQQQTLLTHQFAANGSTMGIIDPGFTAFQNEVSSDLSKPENKNVKPPYSYATLITQAILSTEEGEMSLANIYAHISSNFSYYQTGTSGWQNSIRHNLSLNKAFEKVPRREGEPGKGMKWRVAESFQREFLEKYREGKVSKLKRGSSVVRQLQLHISKFGSLPIERTDFRTNGNFQSKSNEAGNSANTTSKLKHERVDNDQSSNEVQFDESAGNNGEQNASKKAKLSKTRNEQQLTNSTYPNYQQQNQNNYFQQNQANHEQHQPYMETTGNQNNPDQTLWTNPTQRQEQDQQRSVAQAMDSSNSSSNASGSTNQNIPSYSRSQQPQYQAPLQFNSQKVLLSPAKRPASLNTISISGGQHSYSKPSSANNSPTRSGNPLLNVNPLLQQLQPQYSQSGNALQMPKNLSEGQDSSIDNDTAQDSNLKSPSSKLFSISAVEAYTPERGSTLQQVGSEHRRQSSDALKLVSNYPPQHTQTNNRTGEGINDTATSFNRSTTPLHGSGSLLPPPPTGVSRGGKINNGTKNLSADTNTENNLDNLEANAPENGNSNTPVFLQPTPNLKYTNNQQNNNANNVNNNNNKDLNGLPNPQSSPGVWNLLQFSSLNNTPAGRSHSVHPLSERKNSNLGASNGVSGRDDTDASTITGKENHDEPSTLNNNEHATNTKEVPTIEVSRDAGGACNDNNDDNDQQAAGYASSPLKRKATLDAAGDESLSKRVMLDPSSVIVSTAKDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN37</accession>
    <name>A0A1E5RN37_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Activator of stress genes 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g797</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
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        <property type="entry name" value="Stress_Response_Reg"/>
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    </dbReference>
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        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
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        <property type="entry name" value="ACTIVATOR OF STRESS GENES 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47540">
        <property type="entry name" value="THIAMINE REPRESSIBLE GENES REGULATORY PROTEIN THI5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="14"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
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            <begin position="48"/>
            <end position="144"/>
        </location>
    </feature>
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            <end position="219"/>
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    </feature>
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            <end position="399"/>
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        </location>
    </feature>
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        <location>
            <begin position="856"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="949"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1043"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1217"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="69"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="122"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="163"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="183"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="206"/>
            <end position="219"/>
        </location>
    </feature>
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        <location>
            <begin position="805"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="856"/>
            <end position="876"/>
        </location>
    </feature>
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        <location>
            <begin position="949"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1058"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1107"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1147"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1160"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1219"/>
            <end position="1241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ88295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1241" mass="139228" checksum="FB82AD2F6DEB3897" modified="2017-01-18" version="1">MPTNPTKRQRNAKACDECRRKKVKCDFLEGGPKKACFSCSLQGTPCVFSPSTKSTKRKPKVAQKETPGNKKQVTSQNTLKVENTEANGFQTNKNDLRNFTADFSSGFDDAPDIKTKHSKNQSSKENHFGEKLNIDSPADSKPASLYRNLDENLHHDKEGSRMAKSNISAGVAPQLSTSKPNLKRPDTHIQDLKENNNNNNKQTKKPRTEKSKHAMESEKSKKYESLFKMLLPGLPNLEKLNMDLFLKIVENMKNSSNEFKNDMSYSENSVDYLKLAVEQYNYVTHNRVPIEKVSSFNFCLQTPLPPKEVALGFIEKTSQECCVLFRFYHRPTFSKNLDDLYRLSPAEYTSELKTFLPLCYSVMAVGALFSKSSNNTSQDPSKKPQNRRSSRNAPMTMNDKDLADEGYKYFLAARRLIDLTNARDLYSIQTIVMLFIFLQCSARISTCYLFIGVAMRSALREGLHRNIPLGTPGFSLLDIEMRKRTFFSIYKMDVYVNTMLGLPRTISQQDFDQTLPLEVEDDCLTHDGIVSPPQSEFSNNDSRNNDSEEPFEKNTLSSACIANQHTKIIMILDEIVEGLYPIKKANNLISHDFVTKLESKLNDWLTQLPEELNPSISDFSKFSASSSKRHFLYKANRLLRLSFLQVQIVLYRPFIHYLTFKNSKNRYHLKPDELSIKRADNCLGVAQTVITLAKEMMDNHLLSGNYWFSVYTIFFSVAGLIFYIHEFVPTTEVEIAKYKRCVQLSKIGKDILDVLKNSSMAASRTYNILNILFESLNKKTKTFMLQQQLLEEQQLKKQTQQQGQYSPQGNQGPEVSNTMLASPLFLTKQDSNLQHTGQPGAASTDNAQKNTNTIASSLLGTTPDSQTMENTSNKNPLASKITIEQSLEELYNMDFSNFEQFMNDTSLKDNNTMNNVFANMSPIANTLQNANIATDGKIFADNVNSYLSKSGNSSNSSTSSGNPQNNSLNFPTSSDLHPYLPRSPLQFSSVGNQSIQNLPFNENAVTASSMSNSSSMLKNSTSFPLFPKTNITRVSKQNIHSSINEDSMNSGEPIDSFPNATYGSSSGNPVQPQVPSKNISKPSFSGADSVTETNSNKLNDSGKENKNNASDSLGFQFDIKSTTRNKNATSQNTTAYNHPDSNDETSKNNSAFSNNNESNPIGTGNMTQQASDSNKKDNINEHINVTDGTNNAEDPLYLTGVFDQLDMQLFGRFLPPYMSRTNETDSVQSTTLNDLTSENKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN40</accession>
    <name>A0A1E5RN40_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g964</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN40"/>
    <dbReference type="FunCoup" id="A0A1E5RN40">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RN40"/>
    <dbReference type="OrthoDB" id="4041975at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="239" mass="28513" checksum="955FA408346040F9" modified="2017-01-18" version="1">MAFTEKYLEWEEYIIEQLNRIPHMDKYTYHKTSSRITLGLLVLGFIAIINELYITIDMWVISKNTYDELRTSKLENLINHEMLLNEKKYFGTEYKNKKGQIVENFEDMKEFYAKPVHVAHLYVACAVLDKSKGYQPVIDQPLRFHFEFSPEEFEGGRYRPEYGCSLKFLKTKIYHFFKDSELFEELIKKGYKFDNFKISQNVHVFNPFDESLRSSKLDDLPLCFLKIETGDRLKCEITI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN44</accession>
    <name>A0A1E5RN44_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Transcriptional regulator CRZ1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g871</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN44"/>
    <dbReference type="STRING" id="56408.A0A1E5RN44"/>
    <dbReference type="InParanoid" id="A0A1E5RN44"/>
    <dbReference type="OrthoDB" id="3973088at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000630">
        <property type="entry name" value="Zinc finger protein 180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF2">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="492"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="520"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="78"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="142"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="362"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="432"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="107"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="142"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="165"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="434"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ88309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="653" mass="73788" checksum="FCF684112EDFE675" modified="2017-01-18" version="1">MSSNKLSNSSDEKVIWNTQENDTPTHSSENQNFLDWTDINTDDQNLYSDLQRQDRKHDFLRDPSMQVLSSAEQNYLKRQGTGSSNTNSANAFLQSSNSQHGAAEIPNESSKTSETFSPVTTPTFNIIPPLEVLELEKKNQNNFQSSRKVPNIVLSSSGPKPHARLSQNNPTQQNTRISSASNNKFNSHQLSTDSVSNPGYVDSTATINNFSSENFSSSPLLQPIECTRNDLFLNPYAGNTIHPTISNDSSFVTANDLQNSPRLSVLEDPEDILSLQNSPHIPALQVEDFSSNENLLDNFLSLDDSLTVPKTQDFQSFLSEEELDKMINQSLSPARSTSNQGTPQDLSLNAYDYNDLDVKRETSPNMHLLSPKDALFEDSRRERPRSRSISKNSASHSRSRSISRDRSKIEQLENELSAAQTSLNQTYLETTEPTAKKNDNYEVEGHEPSLSHQGSSFDYVFNTGLSEDAPSMYEVDEVTGLQFIDKADKGFYNCLYCDKSFPQAYNMKSHLKSHTDIKPFKCSHCDKSFARQHDRKRHENLHSGQKWYICGGFLEDGTQWGCAKQFARSDALGRHLKTKAGKRCLQSLYDEFTRSRFVLNPDRETLVQNLDPYLDLSLRLDDSELEHTVQPIIEHNFLKLAQKRAHALKNVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN68</accession>
    <name>A0A1E5RN68_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MED5</name>
        <name evidence="11" type="ORF">AWRI3579_g884</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN68"/>
    <dbReference type="FunCoup" id="A0A1E5RN68">
        <property type="interactions" value="229"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN68"/>
    <dbReference type="InParanoid" id="A0A1E5RN68"/>
    <dbReference type="OrthoDB" id="5322661at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014801">
        <property type="entry name" value="Mediator_Med5_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35784">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35784:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08689">
        <property type="entry name" value="Med5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1087"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1097"/>
            <end position="1108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ88325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1199" mass="137271" checksum="47EE1BB262A63CBE" modified="2017-01-18" version="1">MDTASIFDQFRNLIINSVENQCSPNQYFIFYNEFFNEKFGIALKKTEPTLSASTGDTNLITDIKNESFPEDAIDSIKAATPPPIDDEQKKKIYEILSSCFIQTLRELSTRSEHFVTVTNYLVQVCFTDYNSSFVNNITEKIYDLGKENEAIVIYFFTKATNYFKSLSDQLIIDDFLVNDVPAFLLPNLVGNYGEMKSRQLFVSLAKLLISMLSKCELKEGNKFQFKDQSLYEKVMNFCESLSETSLMLSKNLYNLIESKFEIAEWKKTHMHADKSPFAIPVETPSITSPLPSALPGTLTSSKQGSILQQSSSNKHFYDVKLIRYYKNMWLNNKIQHCTTDEIDFFPKYQGIEMALNSNNPILPFESAIVDFIDTSFTCFAQFLNNKKYHQALNSNFTLLEKKWYLFIVKRVPLLVEKYCGVNRSYLAVKAVQDLDDKIIKVFRNYYATGRSNSADNDSTDLFDNFDSPSQPELDIRHDFLKSLCMMKLQNPSVLNKVFSNGDEMIDTNTITDHDLVLVKNKQNNFETIHNLKEYLENSFDSFDFESLCFHDTCISDKSESFDIIYMFQNFESISPNSQLIIAETLVNIMETAVEKFDYKLVAKVCALLSFNLGNSLPGIFALVNPNRLVIPLIKFLDDAWDEHKEQVFSTGTENENDSDVESFNDYIYFGFALVFLIYLQEQYKVDVIQTLVKITDVKKLETSFLLQYMQTYKISSKELKLPSTKEPYKVAKLLQDWTNSLFVENSISDELIKNADIKDLAVLVPFMFDQVISIFQSLVTTSIQQQPQNKNEFNAALANFNTIVVNSVEYFSQPFMMIGLVKIVFTLESTIKTLQSICISQDESLLDTFLNILTPLFNESGILSSESKVLQFILYRVTGPRLLLTMKNLKNLKAKEITENSATVNAKLDYLISRLGRICEAANMYDLNPSILEIDPNEPSPFENQDLSPKSSGSSTHTPVYFDFEITSDAPMNKIMNKQCNSFWNLHSSTFYNYDYLQKIIQIVGPDKFFSDIYRSLVFKVETNGIPTAGNTLKKGEKPGDDVDVAMPYLIYFSILLDFNDNNERLQILQFLESPLSSSFVESLESESTDAATTNKTKTEPDTAKASEETANDNDFDMLFGEDSTDNIDDVLMSDSSTKNEGTNLTNCGAAEKIAKFSFLKKNTFAYLFYQYYRNKAYPFESFAKFYQKYLDLLKRSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNI0</accession>
    <name>A0A1E5RNI0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PRA1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g837</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PRA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNI0"/>
    <dbReference type="FunCoup" id="A0A1E5RNI0">
        <property type="interactions" value="289"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNI0"/>
    <dbReference type="InParanoid" id="A0A1E5RNI0"/>
    <dbReference type="OrthoDB" id="63113at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004895">
        <property type="entry name" value="Prenylated_rab_accept_PRA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19317">
        <property type="entry name" value="PRENYLATED RAB ACCEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19317:SF0">
        <property type="entry name" value="PRENYLATED RAB ACCEPTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03208">
        <property type="entry name" value="PRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="72"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="124"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ88434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="175" mass="19664" checksum="57FEF4BBD5F4F728" modified="2017-01-18" version="1">MNQLTQLSQITSFVQNLSLDNIKMEIANIQGRFSNLRGPQDFFDFKRVSKPANFSEIQQRVSYNLRYYGTNYALIVGCLFIYTLLTNLLLLFVIFFTLFGIMGINKLQGQDLVTPFGTFKTSQLYTGLLFVSVPLGFLASPIATLMWLIGASAVTVVGHASLLEKPIETVFEEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNQ3</accession>
    <name>A0A1E5RNQ3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Nuclear protein STH1/NPS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g801</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RNQ3">
        <property type="interactions" value="1103"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNQ3"/>
    <dbReference type="InParanoid" id="A0A1E5RNQ3"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140008">
        <property type="term" value="F:histone H4 reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04369">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17996">
        <property type="entry name" value="DEXHc_SMARCA2_SMARCA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000008">
        <property type="entry name" value="Chromatin structure-remodeling complex subunit snf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000843">
        <property type="entry name" value="Chromatin structure-remodeling complex subunit snf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014012">
        <property type="entry name" value="HSA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029295">
        <property type="entry name" value="SnAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07529">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14619">
        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01314">
        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51204">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSA" evidence="14">
        <location>
            <begin position="334"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="508"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="820"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="11">
        <location>
            <begin position="1393"/>
            <end position="1463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1123"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1188"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1304"/>
            <end position="1367"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="351"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1128"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1219"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1304"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1341"/>
            <end position="1350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ88522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1482" mass="168557" checksum="5FC1E0E5337ACBD1" modified="2017-01-18" version="1">MTEDMNNAVTASANLNSTAVPAAGVQPFPAQDLSPNRASIEEIQLPLPASRREMNVLLKRYEELNKDDAENKKDDVNGHDLLKESDIVKICKNTELKSILNVFDQISMDEDSYLEKTNEINKTFVGNTADHSVDIDLLQKQLVGFQLLSKELYFPDQVLTQPLKSELQKTQLDTESTLEIPSVSDFPGIAEFFQLGYTLLNQQDLTKPHLGISDIETKVSQQIAKRIKYLENIPANVATLSLKDALKFYNSDEIPDDIDKIKLKAVVELKTLQLLTLQKHLRQKVLHTVAGQSHYKHKYLCDNPITLSAQRSVAIREKKAIPHTTKLIEDLEHQKRLAALQEKKNLHLLKLKSVIEKVAGFENNIQNKKTNLQYLGSSCLAYHQIAEKEEQKRLERTAKQRLQALKSNDEEAYLKLIDQTKDTRITHLLKQTNVFLDSLAKAVKIQQDSVKVTSDAEGMEVESLQDDREKRDYNSAAHKIKEKITKQPSSLVGGILKDYQLKGLEWMVSLYNNHLNGILADEMGLGKTIQSISLVAYLSEVKKEKLPFLIIVPLSTITNWTIEFEKWLPSLRTIIYKGTPNQRKTLQYDIRVGNFDVLLTTYEYIIKDKALLAKHHWAHMIIDEGHRMKNAQSKLSYTLTHYYKSKNRLILTGTPLQNNLPELWALLNFVLPKIFKSSQTFSEWFNTPFAKSGTQEDLSLNEEESLLIIRRLHMVLRPFLLRRLKKEVEKDLPDKVEKVIKCKLSALQQILYQQMVKHNALFVGQSKESKSGIKGLNNRVMQLKKICNHPFVFDEVEMILNPTGKWDTELLWRVSGKFELLDRVLPKFKASGHRCLIFFQMTSVMNIMEDFLRMRGMKYMRLDGSTKADDRQDMLKEFNAPNSEYFCFLLSTRAGGLGLNLQTADTVIIFDTDWNPHQDLQAQDRAHRIGQKNEVRILRLITNDSIEEIILERAMQKLDIDGKVIQAGKFNNDATNEEREEFLKKLLELEANRDMDADPEMEDDEELNEIMARSDDEKVLFAKMDAERAEAAKNFALAAGLEEPLSRLITKDELPEVFTQDVAHHFAPDANKEKLGKARQRKRVFYDDGLTEEQWLKAMDDDEDSVEAAVQRKRSRMERRLEKLGVDGEIDGIGEDAEEDDESNDEKDPDVVDSASPRKRNRRIRKISFAPAPIVDNVLTAVVDENGEEEYTPEADGNTVSSIDDVVSHNGHANTNGRGRGRGRGRGRGRGRGRGRGRGRGRGGFASTGRYAVGEEVQDQSNIAEPIVADGVSNGTKTLVEIQEPDLVNQKLFVKIKVKIPETENSVTKESKSTSKRSSRSAKNSSSELQQDIATPGDETASLSTSNTESAPKANAPVGKTKRTRKSAFNASPFNTKLIELVRSLRTLLDSSDEHTRTGIFEKLPSAKLYPDYYNVITSPVCLKTILDKSRKGEYINVDQVKEDFATMFKNAKTYNEEGSWVYNDAVFLDEYIDEHWSEYFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNZ6</accession>
    <name>A0A1E5RNZ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tubulin-specific chaperone B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g755</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNZ6"/>
    <dbReference type="FunCoup" id="A0A1E5RNZ6">
        <property type="interactions" value="706"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNZ6"/>
    <dbReference type="InParanoid" id="A0A1E5RNZ6"/>
    <dbReference type="OrthoDB" id="5295208at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CAP-Gly" evidence="1">
        <location>
            <begin position="153"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="246" mass="27947" checksum="65B2AB8BDDE6BE9F" modified="2017-01-18" version="1">MSSIQLLGPFTNTMEFTTDFLKKIGQNNMESLFKELHIKTGVDIDSMIVKLYCATSEPAVLELTPGGKSRNNAEKSTINFHTISKIVVIDTNKNSVANQLSREDNFDQDQDQVLTKEKYQQLDDSVYKWKQNLQNEIQLSIEKTVQYYRDHCIGKKVQITKDGRVGMLRYVGCIKELEKPKQYFCGVEFTQPIGKNNGCFQNNLETRYFGPVAHNHGVFVPLAGIRVLPDASEQEQVQDDGGIEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP33</accession>
    <name>A0A1E5RP33_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein BRE4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g608</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP33"/>
    <dbReference type="FunCoup" id="A0A1E5RP33">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP33"/>
    <dbReference type="InParanoid" id="A0A1E5RP33"/>
    <dbReference type="OrthoDB" id="1924968at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023244">
        <property type="entry name" value="Brefeldin_A-sensitivity_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052430">
        <property type="entry name" value="IVT-Associated"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47804">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47804:SF3">
        <property type="entry name" value="PROTEIN BRE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02047">
        <property type="entry name" value="BREFELDNASP4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="257"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="308"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="342"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="409"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="771"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="822"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="845"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="911"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="191"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1178" mass="134581" checksum="7078826A92D86D97" modified="2017-01-18" version="1">MSGLEEVDDNRQGSGSYRQKSFLSASLRSHHGSGTNTHNNSFSQLPTQETMFKNSLIKKFGLTSLNQNSNNKDESFMQLPPASAASQKFSLSNSNETFLHSRTSQSQLQLNQLISNNEALEKTYLNAQPSFLSNEHTFHHENNAELNQNGTGLNDYELEELRDGFFDPVFPKHTPVRPLIDENIAETAQASYIEKDGEDEKDTGKSTSDGTASTKGFLPMSNAPTRPPSQTAFQKVLGTFAFVKINSHKALKKRKKILKFFIAYFIAMVLSVIPKTRSWFGSDRYIWFLPLATLIHHPSRTVGVQVEIAVWAILGGSLSIAWSALALYIATCNNAALRGYGGILWLSMTLAVILSVWLGSLFRRLVYFSTAFNTGILFTHTVSFINAFPQVAHIEINNSVRNWKIMWDFGISYLFGILISTTVSFIVFPDFGNNIITDTFAETIESIENFLVLFIDPKNFENKEQLASKEKTMVRKMNYKCTQAYREYSNQLKLSRYDEHLLKNVRNSLTNIVAPLRCVPLNLSNIINQEALENLISGINADGINFDAEDTDYENLADESTALQTTLQKPLFDLVLQMIICLDYNKEMFKTPNAQNNCATLHKYDVVLQEKIQNTDKAYKSFIKSKFFNKELLRDPKCVEMFLFLRYLVHSANHLVVLNSTTIETIGTQRWHVVPPSYPLSTSLRRLPHQCALDQGNGDQMNYSDTKRATDEIFENIYNANMSKHIFQASTSDKHVKAISRNDFNKKTTTSSARWLFWNFKCAFLCDEMRWALKVSAVMFFFCLPSWLPNSYKWYQKYQVFTGAILLNVLINRRNIDNWESFAVRMINCLIGVFWGWAANQSRHFASPYVIMTFASLLCAVYAYVFFVNRNTKISYTALLTFTIIALEPLGNKNVYSHHSVNTATIWKSTWVTGLALFFGCALSIPINWVLWTLSARTQVREGANALVGHIGKSYQFLVDRYLYRDVDDEPSLLELKYSNVSEIRLSQSIDALEELLNQAKKESEYITKFKATQYEQLIQCSRALVEKLLESRLSAQFFDVWNTDADSRVTRSLLSFRRDSVSTVCFVFFMLSNCFQSKNKVPPYLPNPILSRKKLYDAISRFDTLDWNGRQDSEQSSVFSTAFNFSPEGKVSFKLFEKRHWQEVHAIAFSSAFTGVTAELQRMIEISKQILGEETRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV68</accession>
    <name>A0A1E5RV68_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcription regulatory protein SNF11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g454</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RV68">
        <property type="interactions" value="96"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV68"/>
    <dbReference type="InParanoid" id="A0A1E5RV68"/>
    <dbReference type="OrthoDB" id="4067384at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="162" mass="17984" checksum="CB357DC6C70D3B4F" modified="2017-01-18" version="1">MNQSPTLSVPSQXITDQSTNASPSTQQLQYKIQILLHINSLLLSKLYTQQQTLLLQQQQQQEQTTEQKIHSDYLKRVHANLQCISQLHQGVSGAKPSIMEPPVSYYSVGSGDGHAGSMGGSQVGQTNNAPGHTGSTNDALIKQQEEGLRRLYLLLNRMFEIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVF0</accession>
    <name>A0A1E5RVF0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein-lysine N-methyltransferase EFM5</fullName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Elongation factor methyltransferase 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">EFM5</name>
        <name evidence="6" type="ORF">AWRI3579_g548</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that trimethylates elongation factor 1-alpha at 'Lys-79'.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. EFM5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVF0">
        <property type="interactions" value="287"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVF0"/>
    <dbReference type="InParanoid" id="A0A1E5RVF0"/>
    <dbReference type="OrthoDB" id="206354at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03187">
        <property type="entry name" value="Methyltr_EFM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019369">
        <property type="entry name" value="Efm5/EEF1AKMT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041370">
        <property type="entry name" value="Mlase_EEF1AKMT1/ZCCHC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13200">
        <property type="entry name" value="EEF1A LYSINE METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13200:SF0">
        <property type="entry name" value="EEF1A LYSINE METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10237">
        <property type="entry name" value="N6-adenineMlase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0808">Transferase</keyword>
    <feature type="unsure residue" description="D or N" evidence="6">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ90901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="245" mass="28053" checksum="C647E20C41909BE7" modified="2017-01-18" version="1">MSHSDSDDELTLSAATLAALQEFRNEETQRQEEFAKLANAAEEDFESTKHKGIHLFKEDWQLSQFWYSDETADTLADALLEGADENTVIAIVSAPSVYAAIKKRDEKTLPTEHIYLLEFDKRFEVLAGSDHFFFYDFADPTNXSDKLKGKVHRLLIDPPFLNENCQTKSSITAKSLLVSDKSAKTKDNILQYRLISCTGERMKDVIKKVYPDTHITSFFPEHANGLSNEFRCYASFEWSKWKFAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVI7</accession>
    <name>A0A1E5RVI7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA replication fork-blocking protein FOB1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g391</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVI7"/>
    <dbReference type="FunCoup" id="A0A1E5RVI7">
        <property type="interactions" value="292"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVI7"/>
    <dbReference type="InParanoid" id="A0A1E5RVI7"/>
    <dbReference type="OrthoDB" id="3863715at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="508" mass="58449" checksum="22935C2D64B79C31" modified="2017-01-18" version="1">MFRGFASNLIKKRENKTNTQPPQALQIEMTLKSEPKSGKKRNAVEIEKLETAKEDDRVDPPTKKQQSNTFAFPKLPLSNMPTSETEAEFLDKLYSTELAAKAGIDMTAVREGHDDILKNFESGHGNLTQAQYDLVIQDNLSEQTLLEHSGGKVTKRNKGIRMTVVSTRYKRVKVGDGKRGTFKVFDIKRDKEFLPPKLIFDAILQTHLLNMHLTADKIYSGLKDKYANVVRDCCRRGVLFCSHCNKDLDIKPFQRPKRMHITQKSGLLPLERVQMDLFKPFSKKEFGTRIIEDKYPYILFLRDFCSRYIWVYPLENVTVGDILPIFKQFLMSTAVNVPIYLESCTISSEMMKNLIEYVCSEYGLKLGCGLGRTASFQKCGIRTIKKYLKQRADECLNDWGGVLQLIHEYNNEYNSVIMDIPRRLLRVTFDKTKEFETKKTSVLEALPASSFKRYYGKSGFFYVEPESSKFSYNEDSNEFIHDEESNPFDKEITQSSINTALNDENVSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVN0</accession>
    <name>A0A1E5RVN0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NASP-related protein sim3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g494</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVN0"/>
    <dbReference type="InParanoid" id="A0A1E5RVN0"/>
    <dbReference type="OrthoDB" id="5587616at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051730">
        <property type="entry name" value="NASP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019544">
        <property type="entry name" value="Tetratricopeptide_SHNi-TPR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15081:SF1">
        <property type="entry name" value="NUCLEAR AUTOANTIGENIC SPERM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15081">
        <property type="entry name" value="NUCLEAR AUTOANTIGENIC SPERM PROTEIN NASP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10516">
        <property type="entry name" value="SHNi-TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Tetratricopeptide SHNi-TPR" evidence="4">
        <location>
            <begin position="214"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="69"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="342"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="77"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="129"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="142"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="343"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="366"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ91000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="388" mass="44913" checksum="39232AE13958A476" modified="2017-01-18" version="1">MSKTEREMNQLLVEATKYYKNDQFEESCNVFSELNELYYATNNDNNPYYLFLYGRSLYHLAVESSDVFGGTGARGEEGDEEDEEDEKEEGTQKKKNDTKSTLYQFGEEVEEEEEEEEEEEEEEEEEEAGAEKKETNEKEXXEXEEEEEEEGEEEEEKVEDGHEDQEEDSDGNDFENAWSVLEISRAMYEEQLQQAQASEDVKLVKDLKLQLSENYDVLGLVSLEMENFKQSKDDFVKCLQYREDVYQQVDPTNRLIIEANYKLGIALENDPLEYKECEKYMKKVINLLETRIKEGNGEPDDSDLVKDLKEKLEEIKANNIENENMEVKQLQAMKALLGGAVSGHSNSGTEQKVNDLTSMIVKKGKKPDTKKRSLSTEDEEADLKRAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RYX1</accession>
    <name>A0A1E5RYX1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000002">
        <property type="protein sequence ID" value="OEJ92141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RYX1"/>
    <dbReference type="FunCoup" id="A0A1E5RYX1">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RYX1"/>
    <dbReference type="OrthoDB" id="4035606at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.250">
        <property type="entry name" value="YML108W-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015080">
        <property type="entry name" value="DUF1892"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035946">
        <property type="entry name" value="YML108W-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08987">
        <property type="entry name" value="DUF1892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89975">
        <property type="entry name" value="Hypothetical protein Yml108w"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="135" mass="14948" checksum="E053519B18A461EB" modified="2017-01-18" version="1">MSNSNDKPLYQMLILLEHSVDEASSSSKSAETNAKKENTAEATTENTPASDATQENEAAGSIKQHEYVDEISVPVCVGEEHMEELNSWFDKFDEKICIPNEGYIKYEITSDGLIVLLIDESRASLVDEVTQFAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZF8</accession>
    <name>A0A1E5RZF8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g212</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZF8"/>
    <dbReference type="InParanoid" id="A0A1E5RZF8"/>
    <dbReference type="OrthoDB" id="4061195at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="773" mass="90677" checksum="BC95AB265E981D7C" modified="2017-01-18" version="1">MVNKNEQNNPIPGQINTPNRQQKPTQHTHSTMLRAGSNSALLRASLRCQSTTTAKYTPTTAKYTPTLNQVIIKQIQTGQQKPINEIVNALNEKLSKSSATGQVPKERFYSNDFFTNKNISELISRSKYDYKMYLQNKYSFANDVPPTPYELFTKLDNLSLIQPYHYTLLMKYYYDEKNFKELLSFYVNNSNKKEVFDKLRHNNKRESNTLRDMIIISYLQTCSSKENVDFNILLKFLNNNFLQIDHATFRSLVELFYYKGSVKKDPLDTQSMSKFHEFYKQFMDTQSKARFAKSIPPNRTGLSYLIDLKNHYLPYVYQKNKTPKLSWIMDSMMDHYSYKLFTELYKQYPNDCYSNRSLLYALGKNTQDPKEVRSQRIQAVWNTFFVPRTDPPLCKDDYLAMLRALGELDYENKLEHFFKNSVDIPASLRKDVQLENCYHFYLLNYDGKSNFHSLKQMEEIEKTIGTKSQQSLEFVHSHLKELTQVSNDEHLQKLVLILGMRLVEQKSFDKEMEMNSKGSDGKLLQILQALGLENVEKSLIVYNKLLNSENLQDKSLMIEESHFDFAQISSYTKNIKFYENLLRYLPNNYNNPTNYLMNIIETCVIQLCRRNEEDLAVELFKLNYQKFINLANAKNTKNVSRDIKTLINAMVQGLSKNLKYCSPNDPAHISKCLKSLDEFMFLYLKSFEVLFENQQTNTQSQNPRKNGVFLDDRSMKQILMAVVDLKFGKLSHKDKQLLKTFIDHYNQYKDIYDKNLTSVEKAKIENLITKLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZK0</accession>
    <name>A0A1E5RZK0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Autophagy-related protein 18</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g145</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat PROPPIN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZK0">
        <property type="interactions" value="566"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZK0"/>
    <dbReference type="InParanoid" id="A0A1E5RZK0"/>
    <dbReference type="OrthoDB" id="1667587at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048720">
        <property type="entry name" value="PROPPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11227">
        <property type="entry name" value="WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21032">
        <property type="entry name" value="PROPPIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="258"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="325"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="481"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="680"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="741"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="263"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="328"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="481"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="508"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="530"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="572"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="681"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="750"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ92387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="821" mass="89858" checksum="0C58269C2EE06BBA" modified="2017-01-18" version="1">MCFNCSAILDSQKDHIKIKKDPSLLNKLLYPSNNNQLKQSTWAPKTEKTSDKQQNSKVPLFSHFNTKMSSTTHHRDAPVVQYVNFNQTGSCVSVGTNHGIKIFNCDPFGKFYSSSSTTHAVTDPHSSSSTPTDGGYGIVEMLFNTSLLAVVGLGEEVSLSPRKLKLVNTKRQTCICEITFPTAILSVRMNKSRLVVLLQAQIYIYDITNMKLLHTIETSSNPNGLIDVSNNIDNFDYLVYSSSQKLITSEVKDHSIPARRRSFNNNSTXSLSSSFNSSSSPPSSMDTIQEQQQQSTQIITSNGTSSNLHKDIFSTSXDTRIVSVSNQEYNGSSGSTTNTSGYTGSETAGSSNSTASVNGGNNNSSDNSGSNNNNAPISKNGDVIIFNLKTLQPIMVIEAHKGEIAALSLNHDGTLLATASDRGTIIRVFSTETGNKEYQFRRGTYKTRIHSLTFNSTGQFLACTSSSRTVHVFKLSPNSATSGRFTNDLHSSPSQQSLTAKMMHKNKSLSTSGSTSSSIRSNHTGHTNFDESDAESANIIDEDEEEDEDEDEEEEDHNLNVDXGEVGKSDNEDAVGXETGDVEEREKDIQQEEVAPVVDSSRSTMARMIRKSSQKLQRKAAKTLGQYFPQIRVASILEPSRHFASLKVPNFDNLQHLHLTSAANNDVLIFDNMENPAHIGASSSPMNNPSTSTLSNNNNNSNNNAHINSINLRLQAAFGEKLIDIDIRDYPEMLKYKRTAATHAPEHQRVPSSASSSNNTNNVSPITSNSSRASVKAEASXFIKVLPIYVISTEGFLYKYLVDPDRGGDGILVSQYSLLYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZT3</accession>
    <name>A0A1E5RZT3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nuclear pore complex protein Nup85</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g182</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Functions as a component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
            <location evidence="1 9">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the nucleoporin Nup85 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZT3">
        <property type="interactions" value="155"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZT3"/>
    <dbReference type="InParanoid" id="A0A1E5RZT3"/>
    <dbReference type="OrthoDB" id="17644at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011502">
        <property type="entry name" value="Nucleoporin_Nup85"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13373">
        <property type="entry name" value="FROUNT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13373:SF21">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07575">
        <property type="entry name" value="Nucleopor_Nup85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7 9" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="57"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="95"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ92346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="832" mass="95181" checksum="414425D58C6CA2D6" modified="2017-01-18" version="1">MSTSARNENQLSMSNTAAKKSSPFQQLLMDPNALSFLDDKEESSEKRSENLDDADIDEHSTQGKAEEIAFDTRSVDMEESEEPKGESANGTKEADADDMDIEAEDEDGESGTKQNGTKGVGQNAEVTQDLEELEGSNKNVPTFFFKFNPCFNAGIFGSGRGFQLTEITNLDCEDEEELDELLNAHSTEPVFNTADVDYISKIFEIYYGLYKSGDKNYLSSNDVLPYGVVNKQEVSEKLSLIDLSFEQVSSELDYYLTKKLNEPEHDDKFIFRLQELNNVLNCLKTFQFIQFEEYLGTEFFTRLHEFINKLDGQPSTEVIYEFLEGEPTKLAETETFYKLVIQLLLRGLIEQAVNLLKTWQEKTKSHYISNIDAFNSVVQLLENYPYGNQNHFREWKNYVAELANYLENNPNESDIKGETKWFNKMVHCINGNQNVISSVSTKWYETLSGLFHFYIPTIKLLPEYLAIALEKYTDNQLTILEENLIYILENRIVNILPYLESLHQPSSAFVAALCEHRGLLPFLPSLANDKNERTVSSYMLYQLGLYLATPSAKISDALLKRLYSLAFGVLKVSNYEPYLRDTISELLPRYPVVTNEDMEYCLTIAAKWKLPATISVIYQRLGMELIHTSWLESMIFFSKSMECSDSSVSDSVGESKLIYYSLLLLEHSLINGKPVDNALVNQVLEXDEQYNAMPKALRQSLAPYRVLYRIFQLVEDQKNNLQSTTLSFEILRLSNELLRFQYLKNYQKVLLTAKIVYPLLREVSTTHVKFDSQLLIDVMESVNNLEKSIFAKDSKDAAISDRLLGDQLETDCIGFLKTLNKQLNQLFCTSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S082</accession>
    <name>A0A1E5S082_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g113</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S082"/>
    <dbReference type="InParanoid" id="A0A1E5S082"/>
    <dbReference type="OrthoDB" id="5308060at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013952">
        <property type="entry name" value="DUF1776_fun"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08643">
        <property type="entry name" value="DUF1776"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ92496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="875" mass="97550" checksum="39998C0270DD580A" modified="2017-01-18" version="1">MPFPINFRTFSNKANLPISPISLTNVSAASVKNNEIAQTPDGKEAQATDPKAKVSKNAQLKKALQKICRKLCSKKDRKKELPTDLTMGTQRFATNDPTMTLRGPSSRTGPSAPLDIFALFALPVSPDTDDATSVEITETAPQPRVSRAKKAKRVCCKKIKTLFNFKKRLSSNCSVSNIQALPESAAASFHSEEVEEKECVSAEEECVSADEECVSADEECASENDTQQLRSILKTTSKYDQPSTPVLPERESMNIALEPSVEALLISYKNALSSLEAYELSLVTSISRKEQPLDWRSLRTPAPQDSSSHTPKTVPEWKLVHLKAHLNKLDEGKNYVVDNTVNNGKKIWSSTSSKYEEVKNYVNDKLVFEESKISASSMGDSGAEVDIEHLGYAPVGKVSIGTRASATTSEYSADDGFFADKFKKLKNLHNSKSSSGSIAAGSAGGAKPCCGNLGLSHLSSLSKTDLFCLVGGVTSTALCVYGFRRYCYNKLNKTXSYLSYKDYNNQIILVLGSMVDPITRAQVYDLYKKGFIIYVCSSNVTAFGEEDDEDILDEADDLNDKRKHARAEEEEWVKSSDEYNNNDAXMIRLNYISTKPQDLSKLIKIIRARNWRLCSIILSHNNVSNNINLRNFENGINNNLIQLIKVIYKITPFFRDVKVILLNPSFSLLIEQSSAKKPDGAQSSLETFSSHIIVGLFESLQSIPDFKEKNNSVYMVHLGLLSFGNNGETLANYKYLQQSPSVYGFGSLNDEYRESLDSNNASLSLLKGGKIQETFLNPVANLIMGKHALLRFLSRQCTRNVMYCGSGSTLFMKYDSWKCLRNPTAFKVWINSQIYLNYFGLWFGKTFDQLWLKSDFFFKNLYAKVLSATQKLIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5QZZ4</accession>
    <name>A0A1E5QZZ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 14">Sterol 24-C-methyltransferase</fullName>
            <ecNumber evidence="2 14">2.1.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Delta(24)-sterol C-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g4645</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of zymosterol to form fecosterol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>zymosterol + S-adenosyl-L-methionine = fecosterol + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21128"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17038"/>
            <dbReference type="ChEBI" id="CHEBI:18252"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 14">Steroid metabolism; ergosterol biosynthesis; ergosterol from zymosterol: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12 13 14">Belongs to the class I-like SAM-binding methyltransferase superfamily. Erg6/SMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.41" evidence="2 14"/>
    <dbReference type="EMBL" id="LPNM01000017">
        <property type="protein sequence ID" value="OEJ80232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5QZZ4">
        <property type="interactions" value="319"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5QZZ4"/>
    <dbReference type="InParanoid" id="A0A1E5QZZ4"/>
    <dbReference type="OrthoDB" id="540004at2759"/>
    <dbReference type="UniPathway" id="UPA00768">
        <property type="reaction ID" value="UER00760"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003838">
        <property type="term" value="F:sterol 24-C-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000121">
        <property type="entry name" value="Sterol 24-C-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050447">
        <property type="entry name" value="Erg6_SMT_methyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030384">
        <property type="entry name" value="MeTrfase_SMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013705">
        <property type="entry name" value="Sterol_MeTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44068:SF1">
        <property type="entry name" value="HYPOTHETICAL LOC100005854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44068">
        <property type="entry name" value="ZGC:194242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08498">
        <property type="entry name" value="Sterol_MT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51685">
        <property type="entry name" value="SAM_MT_ERG6_SMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4 13 14" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13 14" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7 14" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="11 14" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="8 14" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="10 14" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="5 13 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase Erg6/SMT-type" evidence="15">
        <location>
            <begin position="84"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ80232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="384" mass="43418" checksum="9D74752F974E23E9" modified="2017-01-18" version="1">MTESYTTQREVNGDVAQLRKRQADFAKELHGDDSSKTGLFALLSKSKDAQKEAVAKYLKHWDGKTDKEAEGRRLDDYNEGTHSYYNVVTDFYEYGWGTSFHFSRYYQGESFSAATARHEHYLAYKAGIKPGDLVLDVGCGVGGPAREIARFTGCNVIGLNNNDYQIGKAKYYAKKFQLQDQMDFVKGDFMNMDFEENTFDKVYAIEATCHAPKLEGVYGEIYKVLKPGGTFAVYEWVMTDKYDETNPEHRKIAYEIEIGDGIPKMFTLDVARDALHKVGFEVLEEEDLADPNAQVPWFYPLTGEWKYMQTLADVSTFFRTSYLGRKFTTCMVXLLEKFKIAPEGSTAVTNALEEAAVGLVAGGKSGLFTPMMLFVARKPLEQEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0B5</accession>
    <name>A0A1E5R0B5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial import inner membrane translocase subunit TIM44</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g4559</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tim44 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0B5"/>
    <dbReference type="FunCoup" id="A0A1E5R0B5">
        <property type="interactions" value="1041"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0B5"/>
    <dbReference type="InParanoid" id="A0A1E5R0B5"/>
    <dbReference type="OrthoDB" id="10265990at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.240:FF:000002">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit TIM44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017303">
        <property type="entry name" value="Tim44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039544">
        <property type="entry name" value="Tim44-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007379">
        <property type="entry name" value="Tim44-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10721">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10721:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04280">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037871">
        <property type="entry name" value="TIM44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00978">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Tim44-like" evidence="13">
        <location>
            <begin position="250"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ80345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="410" mass="46456" checksum="6CE3510F35078297" modified="2017-01-18" version="1">MIRAVSRVGIHAGSKRTLHTTCVLLNGGGKSPLQIFRDTFRQEWQKSKELQENIKQLQDASGKIADSEALKKAREASARSSTMVSQTLKKTSEKLDNIANAAWDSEVGKGTRKAINKTAETIDNTIIDPVKNTKAYKDVSETLNNGSSTAYGGFISKEQRDLLRAKQLSTRKHQSTKGNEEAGTALVATDIESKTDFQEKLKNFQQQTTLGKFLSSFKTKFWDETENPLIVLIRNVHRKISGFFFAETESGKVIKQFRLMDPTFNMEDFNRYLREYVVPEVLEAYIRGDEKVLKTWFSEAPYNVYAAQQKFYREQKLFSDGKILDIRGIDIVSSKMLPSEIPVFVVGCRAQEIHLYRNVKTGEIAAGTESNIMQSSYAMVLTRDFEKVDDKVTEGWKILEFVRGSSRSFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0S7</accession>
    <name>A0A1E5R0S7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="14">1.2.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g4507</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = (2R)-3-phospho-glyceroyl phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10300"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 14">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.12" evidence="14"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0S7"/>
    <dbReference type="FunCoup" id="A0A1E5R0S7">
        <property type="interactions" value="1293"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0S7"/>
    <dbReference type="InParanoid" id="A0A1E5R0S7"/>
    <dbReference type="OrthoDB" id="1152826at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00184"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030312">
        <property type="term" value="C:external encapsulating structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18126">
        <property type="entry name" value="GAPDH_I_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05214">
        <property type="entry name" value="GAPDH_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000001">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000020">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006424">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01534">
        <property type="entry name" value="GAPDH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836">
        <property type="entry name" value="GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836:SF76">
        <property type="entry name" value="GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 14" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 11 14" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding" evidence="15">
        <location>
            <begin position="2"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="11"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="148"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="208"/>
            <end position="209"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Activates thiol group during catalysis" evidence="12">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ80504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="331" mass="35392" checksum="D1443293995A8FFA" modified="2017-01-18" version="1">MVNVAINGFGRIGRIVLRIALQRKDINVVAVNDPFITTDYAAYMFKYDSTHGRYEGTVSHTSDSLTIDGKTIKVYNERDPANLPWGKLNVDIAIDSTGIFKELDSAQKHIDAGAKKVVITAPSGTAPMFVVGVNADKYAGEKIVSNASCTTNCLAPLAKVVNDAFGIEEGLMTTVHSMTATQKTVDGPSHKDWRGGRTASDNIIPSSTGAAKAVGKVLPVLQGKLTGMAFRVPTVDVSVVDLTVKLEKATTYDEIKAVIKKASEGPLKGVLGYTEDAVVSSDFLGSSYSSIFDAAAGIQLSPKFVKLVSWYDNEFGYSTRVVDLVELVASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R188</accession>
    <name>A0A1E5R188_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Multifunctional methyltransferase subunit TRM112</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4301</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRM112 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R188"/>
    <dbReference type="FunCoup" id="A0A1E5R188">
        <property type="interactions" value="838"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R188"/>
    <dbReference type="InParanoid" id="A0A1E5R188"/>
    <dbReference type="OrthoDB" id="2187549at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070476">
        <property type="term" value="P:rRNA (guanine-N7)-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039127">
        <property type="entry name" value="Trm112"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005651">
        <property type="entry name" value="Trm112-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12773:SF0">
        <property type="entry name" value="MULTIFUNCTIONAL METHYLTRANSFERASE SUBUNIT TRM112-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12773">
        <property type="entry name" value="UPF0315 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03966">
        <property type="entry name" value="Trm112p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158997">
        <property type="entry name" value="Trm112p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="160" mass="17847" checksum="6814CC4C82B9BBD3" modified="2017-01-18" version="1">MKFLTTNFLKCSVKSCDISNDSFPLKYNGETCKIEQTPLDTVTTTNPADGSDIDEEYINYEFLLGIIDRLDWNAVLEVAQDLGNTSLPASKPSLTMDGDFNETSADHNTMINNNKALLRDLQILLMQTNIVEGDMKCKNCGHVYYIKNGIPNLLLPPHLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1B1</accession>
    <name>A0A1E5R1B1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleolar GTP-binding protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g4314</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class YlqF/YawG GTPase family. NOG2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1B1"/>
    <dbReference type="FunCoup" id="A0A1E5R1B1">
        <property type="interactions" value="912"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1B1"/>
    <dbReference type="InParanoid" id="A0A1E5R1B1"/>
    <dbReference type="OrthoDB" id="444945at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012971">
        <property type="entry name" value="NOG2_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050755">
        <property type="entry name" value="TRAFAC_YlqF/YawG_RiboMat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089">
        <property type="entry name" value="GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089:SF9">
        <property type="entry name" value="NUCLEOLAR GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08153">
        <property type="entry name" value="NGP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleolar GTP-binding protein 2 N-terminal" evidence="5">
        <location>
            <begin position="41"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU364023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ80676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="272" mass="31056" checksum="85D25E77C7AA7CC9" modified="2017-01-18" version="1">MGTGKKEQQRRIRQGDTKDGNLRVKGENFYRDTKRVKFLNMYKGGKAVRNADGDIVQSAALQSRDAPTARVAPDRRWFGNTRVISQDALQHFRDALGEKKNDTYQVLLRRNKLPMSLLDEKDTVDSPTAKILETESYEHAFGPKSQRKKPRVAASSLEELLESSNDHIQKFEEKRELDATLGLMGATHDEEEGWTNTAKEAIFHKGQSKRIWNELYKVIDSSDVVIHVLDARDPLGTKCKSVEEYMTKETPHKHLIYVLNKCDLVPTWVAVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1F9</accession>
    <name>A0A1E5R1F9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Serine/threonine-protein kinase MPS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g4523</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1F9">
        <property type="interactions" value="349"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1F9"/>
    <dbReference type="InParanoid" id="A0A1E5R1F9"/>
    <dbReference type="OrthoDB" id="20524at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033316">
        <property type="term" value="P:meiotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000131">
        <property type="entry name" value="Dual specificity protein kinase TTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974:SF21">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE TTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974">
        <property type="entry name" value="MIXED LINEAGE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="459"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="29"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="187"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="194"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ80403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="802" mass="90721" checksum="31C263E18274FE6A" modified="2017-01-18" version="1">MSAHELTSEFREKYLLYRKNKEGSLKNQCLDSNSVSVPHTEEHATSNKASNQKSNNVYDEDEEELVVPVLSNFSAHILKGTESGTSEVLPTGAFNSTNTNETALSNNTLMYKNYENQQFNTLTNNTTFNSEKVRHPVSPRNFEINAPKRSAITTTNNNHNGPLKSSSQLRFFPSPGLVAEAYSNTLSHDKENDANTNNNSDTTNKNTVTSFRRSKLFGTSRLSSLTSGSHLGPAQRAASLPVCXEGADSSYMSNLSPFKKTSQYTSPHVDHTCAPSVSPSELMKQKKSKYKDIDFGNKSPMEYMKDHDLPPEDLGVLRNVYLEKYKEQPNTNTWKPESGSFQTYKKNRAQALVNHTEDNYNLQLSTRKPKMQPHEIIDIPTTNKKRKHVSMAPQVKTDCYPNQDANTFEKLDKEYCRDEPQHGNFKQEYVPLKPPSLVEPLQSSRNSVRNMVTINGVEYEKVELLGKGGSSKVYKIKRHHPSTNYSKNSVTSNRVFALKQVLFDELDESSIDTFKGEIQLLYKLQNRSRVVKLIDYEMLQGLLYLVMECGDHDLSLILSNRLSMPLDLDFVRYHFKEMCLCVKEVHDAGVIHSDLKPANFVFVQGVLKIIDFGIANAVPEHTVNIYRESQIGTPSYMAPEALIRLQDSNASFSDEERNVWKVGKXSDIWSCGCILYQMVYGKSPYAHFQGKDRLLAIMSNDVXIHYSANGLGNVPVPRSVIELIKLCLKRNAXKRITIDEILYESNFLNAVSVTEAFMKTVVKSSVQYGVDNASANKTHNLTDKRIENLTSNIWNRLNELRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1I9</accession>
    <name>A0A1E5R1I9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UBX domain-containing protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4103</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1I9">
        <property type="interactions" value="998"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1I9"/>
    <dbReference type="InParanoid" id="A0A1E5R1I9"/>
    <dbReference type="OrthoDB" id="270602at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02958">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14346">
        <property type="entry name" value="UBA_Ubx5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01767">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006577">
        <property type="entry name" value="UAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF6">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13899">
        <property type="entry name" value="Thioredoxin_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00594">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="2">
        <location>
            <begin position="419"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="498" mass="56241" checksum="2FF4E403BE2C9D3B" modified="2017-01-18" version="1">MSQEDIATFLSITNADSPDVAKQFLEMAGSNLETAISLYFEHGAAPAATSSTPSGNDVPYIPPEEDNYRAPDQAVHETLVDGHTAYFPGLSFGGVGGRFGPLRSTNHPSGIFNQDEDFGVSHHYNSHDIQEVDSDSDEMDFDDSESDAESQYTDEEYVEIDEDGELIERTRRVKRNTDTSRNRPRNNNTRMQPVSHQDKLALLFRPPFDMIEKLTLDDAKVEARAQQKWIMVNIQAVEVFQCQTLNRDIWREPKVKRLIRPNFVFLQYAYDSPLGQAYINFYSLEKPGTDSLPHIAILDPLTGERLKQWNETPPKLDDFIEEIKEFLQTFSLDPSHTNPVIPTKPAXAKPVEELSEEQQLEYAIAQSMENGLNSKNDTTEFTDKSAVPANTSSKAADKLPVLDEFDLIEPVSNPEPPNTPGVTTRVQFRFGDGSRVVRRFKLDDQVRSLYQYVKAELEGFHDCRFTIATPQRESLIEKLDETLGDAGLKNSSLLIEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1J4</accession>
    <name>A0A1E5R1J4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g4080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R1J4"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ80752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="256" mass="29174" checksum="D3B31637D7C8106D" modified="2017-01-18" version="1">MRNXKLKKRLSTASLVSVPIEEEEEPCEAHSVLNCVYCNLEATTKTAYNYNKMLKRSISYNAIAGSKALKTKASLDSLHHPLTRSYSQTSICSASTSKTIMPISDDSFNEFLKSLFKKDDYNEMREKWDSFPPEVKKVFIAKTIVKRKPEFLDPQFDVQDKWLQKNSPSVNEAFQYMASPSMSSPISKPTNNCFMHYKEKMGPRYKDIFPGADGAKISKAVALSYNELPGSVKDEMKLAAKQNYTDEIKRRFFXSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1L2</accession>
    <name>A0A1E5R1L2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Holliday junction resolvase YEN1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4349</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1L2">
        <property type="interactions" value="89"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1L2"/>
    <dbReference type="InParanoid" id="A0A1E5R1L2"/>
    <dbReference type="OrthoDB" id="2959108at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF72">
        <property type="entry name" value="HOLLIDAY JUNCTION RESOLVASE YEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG-I" evidence="2">
        <location>
            <begin position="161"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="623"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="627"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="3">
        <location>
            <position position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="647" mass="74304" checksum="5DE041D86957D516" modified="2017-01-18" version="1">MGIPGFWKVADAFTQTGLSLTDVLLENKNVSVVVDGEGWLEECFNTPSVSSENERSXSPSRERFFNVYAFVSRLKPFIQCKCQILIVFDGIWKPPFKRKRVLQNMESEKSIADYMAEYTEHINRFHMNDEQRDNKDPRFECCDKTKIFISERYNKALAVLESLNIEYLFVCAEAEAFCCHLQRTLTFTYVLSNDSDCLIYGATNILREFTKNGSPASEKDEYTITKTTLSKNVTKXXASFSPEALLLAGIILGGDYNQSGITSIGITKTMKLLEDPGFVXVVSAFEKSFFEQNTSYSELQSVFNDYIDSSCKXVFKRNDYVQKYKQQKWPSEFVLMHYVKPLFNKQTIVYKAAVSHKDMEVEVFENAVGNVSASWLHQTLHELFIVHFFDKWVDDKQQLKKMFKINKENESKYRVRYKSFIEGLPDVQVPLEVASPTKXSPTKVQLEIKEYPYYMWISKKIVSYFARPLVEFWEEEKTIMNSPKKLSPKKRKLNGVSVQKTTLDXLDFFSSHTTPKKPLLDASITPLLTANSALTQPLESAKRQLFLNSESELSSDEQEENMIESESETFKPLNSFQFQVPTVPETRFDLLETLFDDTESFVDAQDDSVQIIEPQNVLVNLPKLSPQAESDSNIKSSPEIIELSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1M0</accession>
    <name>A0A1E5R1M0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Heat shock protein 78, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g4085</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1M0">
        <property type="interactions" value="220"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1M0"/>
    <dbReference type="InParanoid" id="A0A1E5R1M0"/>
    <dbReference type="OrthoDB" id="47330at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19499">
        <property type="entry name" value="RecA-like_ClpB_Hsp104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000017">
        <property type="entry name" value="ATP-dependent chaperone ClpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000120">
        <property type="entry name" value="ATP-dependent chaperone ClpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000025">
        <property type="entry name" value="ATP-dependent Clp protease subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000010">
        <property type="entry name" value="Chaperone clpB 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050130">
        <property type="entry name" value="ClpA_ClpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638:SF176">
        <property type="entry name" value="HEAT SHOCK PROTEIN 78, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="136"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="536"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="Clp ATPase C-terminal" evidence="9">
        <location>
            <begin position="709"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="348"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="10">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="813" mass="92184" checksum="491DC543746554F3" modified="2017-01-18" version="1">MXRSVSSRNSKNILASVAKQTKNHVPLSXAVLSLPLRAQNAQNIEITRNYSIXRKNDSFXIPKQFTLQRPKPQYEQYISKRYIQMKMNPNQGQEEKPALEQFGTNLTELARAGKLDPVIGRDEEIARAIQILSRRTKNNPVLIGKAGVGKTSLIDGLAQRIVSRQVPESLQDKELIALDLSSLIAGAKFRGEFEERLKKVLDEIDKKDGKXIVFIDEVHMLLGLGKSDGSMDASNILKPKLARGLRCISATTLDEFKIIEKDPALTRRFQPIILNEPSVPXSISILRGLKERYEVHHGVRITDAALVSAAVLSNRYITDRFLPDKAIDLVDEACAVLRLQHESKPDSIQQLDRAIMRIQIELESLKKETDPLSVERRELLEKDLNLKNEELSRLTSIWEAEKKEIESIKTAKADLEKARVDLEIYQRENNFAKASELRYAKIPELERKVALNDAKNKEEKSKSTLLHDSVTSDDISKVVAKMTGIPTETVLKGDKDRLLYMEKSLQERVVGQDEAIKSISEAVRLQRAGLTSDKRPIASFMFLGPTGTGKTELTKALAEFLFDNESNVIRFDMSEYQEKHTVSKLIGAPPGYVLSETGGQLTEAVRRKPYSVVLFDEFEKAHPDVCKILLQVLDDGKLTDSLGHHVDFRNTIIVMTSNIGQDILLSDATATEDGRVSKETRHKVIESMKRTYPPEFINRIDDVLVFNRLSKEVLRTIVDIRLKEIDERLVDKRMKLELTDEAKSWLTDRGYDPLYGARPLNRLLHKRVLNPMAEYLLRGQLRPNETVRVDVKDGELFVEPNHEEGEVDEEPEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1T3</accession>
    <name>A0A1E5R1T3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal RNA-processing protein 41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3862</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Component of the RNA exosome complex. Specifically part of the catalytically inactive RNA exosome core complex (Exo-9) which may associate with the catalytic subunits RRP6 and DIS3 in cytoplasmic- and nuclear-specific RNA exosome complex forms. Exo-9 is formed by a hexameric base ring of RNase PH domain-containing subunits and a cap ring consisting of CSL4, RRP4 and RRP40.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1T3"/>
    <dbReference type="FunCoup" id="A0A1E5R1T3">
        <property type="interactions" value="860"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1T3"/>
    <dbReference type="InParanoid" id="A0A1E5R1T3"/>
    <dbReference type="OrthoDB" id="437922at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11370">
        <property type="entry name" value="RNase_PH_RRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000004">
        <property type="entry name" value="Exosome complex component Rrp41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF0">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0271">Exosome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="8">
        <location>
            <begin position="21"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="9">
        <location>
            <begin position="158"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00063066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00077929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="248" mass="27727" checksum="F9E36F601ED7AEC6" modified="2017-01-18" version="1">MSRLEIYSPEGLRLDGRRWNELRRFECTTSTKNSSNNSDGSSYLEMGQNKVIAVVNGPMEPLNKAQVNQEKALLKISINLTKFSKMRRSNSSNKNEKRILEMQTALERTFSETVQLNLYPRTMIEVKLHVLQQDGGMMGCLINSVTLALIDAGIAMYDYISGVSVGLMDQQPLLDLNSLEETSLSSITLGVLGKTEKLSMLLIEDKIPLDRVESVLSIGIAGCHQIRDLMDAHLRRAGAEKLEKLSHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1X3</accession>
    <name>A0A1E5R1X3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phospholipase D1</fullName>
            <ecNumber evidence="3">3.1.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Choline phosphatase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Phosphatidylcholine-hydrolyzing phospholipase D1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g4599</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14445"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.4.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phospholipase D family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.4" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1X3"/>
    <dbReference type="STRING" id="56408.A0A1E5R1X3"/>
    <dbReference type="InParanoid" id="A0A1E5R1X3"/>
    <dbReference type="OrthoDB" id="14911at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09141">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000011">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896:SF76">
        <property type="entry name" value="PHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00614">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="13">
        <location>
            <begin position="464"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="13">
        <location>
            <begin position="764"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="518"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="197"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ80553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1159" mass="133372" checksum="D3328CB486722CDE" modified="2017-01-18" version="1">MESENTKASQNTSVCSSDMMFNTKDGWITPEVKKLFKKMKVTAKCCNPSRCVLKSKFCFSLKLEAGATEQAWTTTKVLENFQQFFTQFEKAKFWHHSQASTLKVLSTSASDLSWAAQEFTILCEAKNGFEPRTEASKSKQELNFNTQLGNFLTKLFVFCVNTKTMEKLLDLFSIPFVDFFLCSVDDICLKKGALFLARDLKIEKKSIKHKLKSLESKLKTFGKNAKHIQRETNDLKKILHTVEEKEKSLKKNEKKPLTEKFLTTKHWVMLGPSYILIFSDTDSLSIKKVFLVDNQFRVDGVDSGHSKLKLSNSEGSITFYFFSSNDKNDWVKAIVDMNNKTVWSKTHRYKSFAPVRRNNYCEYFVDGEKYFWAVSERLDAAKSIVYIQDWYLSPEVYLRRPIEGNESYRLDKVLLKKASQGVIIRIIINKENPWFSSTDSAFAKSKLSLLHKNIFFMRHGKGLKAFTHHEKLLVIDNEIAFLGGIDLAFGRYDTEVHALTDENKNVLKQNYPGIDYENDREKSYPKMKHPLKDSLDRKKSQREPWHDIGVMTIGQAARDLARHFVQTNNHVSESKWSKKLILLVSPADFTDDELQALAAKNKIEGIQLEYDCQLLRSAGPWLLGLNTVECSIQNAFIRVIETSEHCIYIQNQFFITACKPGKTFIQNKIGEAIVKRILRAFKEKTPWKAYIVMPLMPGFPHDILEDKAFALREMLFMHFLSIQEKDGSILGMLNKKDIDPADYVQICSLRNWSTIGPQSSLVTEQIYIHAKMMIVDDRKCLIGSANINDRSMQGNKDTEIAVLIEDTRFVKSQMNGAVYYAGELSNRLRQETSREIFGCNLKLINYVEATFANYRKYAIANYGELHTVACQSQNTVSQDEYVDSAVIEIAMSNAFGIGCTTQWGSVNKQDVSSHAKILGTPLEDFPPLEQHSLFFALNAEQLAASMYTLDIDDIKKANLLKSLLYLQKTPFSQSTKKQIVAKNGSFLSLEHFICRAFKLANMTDPFQFFDPLSEFLWLKIWQATASKNTKIYRYACRCEPDDFIRNKSEHIAFQKYQQIFSKLQDNTTSNEKLDEIFRKADEIILLKITNENERNAFLGLFPTCNENKRNQRKVDSEALLKNLKGHLVEYPAAWLEQIIEDGELFSFYEKNIFARFGFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R242</accession>
    <name>A0A1E5R242_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin-like modifier HUB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g4272</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R242">
        <property type="interactions" value="331"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R242"/>
    <dbReference type="InParanoid" id="A0A1E5R242"/>
    <dbReference type="OrthoDB" id="3881at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039732">
        <property type="entry name" value="Hub1/Ubl5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13042">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="3">
        <location>
            <begin position="2"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00014108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="94" mass="10745" checksum="F6940476B08B8533" modified="2017-01-18" version="1">MIEVTVNDRLGKKVRVKCLGEDTVEDFKKVLSVQLGHTKIPTNYAAHDYNNASQHSSIQHKRLVLVKGQVXLKDHITLEDYEIHDGTNIELYYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2L8</accession>
    <name>A0A1E5R2L8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Peroxisomal acyl-coenzyme A thioester hydrolase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4027</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the C/M/P thioester hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2L8">
        <property type="interactions" value="175"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2L8"/>
    <dbReference type="InParanoid" id="A0A1E5R2L8"/>
    <dbReference type="OrthoDB" id="68328at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03444">
        <property type="entry name" value="Thioesterase_II_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03445">
        <property type="entry name" value="Thioesterase_II_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.210">
        <property type="entry name" value="Acyl-CoA thioesterase, double hotdog domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042171">
        <property type="entry name" value="Acyl-CoA_hotdog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003703">
        <property type="entry name" value="Acyl_CoA_thio"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049449">
        <property type="entry name" value="TesB_ACOT8-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025652">
        <property type="entry name" value="TesB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11066">
        <property type="entry name" value="ACYL-COA THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11066:SF34">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13622">
        <property type="entry name" value="4HBT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02551">
        <property type="entry name" value="Acyl_CoA_thio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA thioesterase-like N-terminal HotDog" evidence="4">
        <location>
            <begin position="37"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA thioesterase 2 C-terminal" evidence="3">
        <location>
            <begin position="237"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ81142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="336" mass="38058" checksum="DA37BA97C9E3CEFF" modified="2017-01-18" version="1">MLLKNKALQSFAYIEKILELQRLSSNTFSSVNPGIVPPAGRVGFGGMLLGQSVLAVLHTVPLNFVPVSLHSYFLIGCDXQETISYSVRSLRDGRNYIHKEVEAFQKNKLMFKSVMMLGKVDERDLKLPIIDNELSKIAMENEFLSKGYVPALSLFKAKLDSLQNAAPKLQEKDTVERFLERYSVGALEYQFPKSFFDDGVYASXXDYYVRLRSSINNDYAKCTSQTHGAGYNNGKDINGNDPRYNYVVFSYLSDAYFLITAPHFHGKHIHSQKFSTSLDHTIHWHDTCLDCNDWLHFYIQNKNACQSGRHLMEGEVLNNSSGKLVATCLQEGISIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2N0</accession>
    <name>A0A1E5R2N0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DASH complex subunit DAD1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Outer kinetochore protein DAD1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g4207</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex DAD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2N0">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2N0"/>
    <dbReference type="InParanoid" id="A0A1E5R2N0"/>
    <dbReference type="OrthoDB" id="5566853at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013958">
        <property type="entry name" value="DASH_Dad1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28025">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28025:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08649">
        <property type="entry name" value="DASH_Dad1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="15" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ81152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="85" mass="9773" checksum="E9DA10D59DAFC630" modified="2017-01-18" version="1">MSSMNDNHFKQERELIIQDIEKTMKSIVSNLDTLNVYLENTNEIGKEFENVSKLWEQFYSGLPYDKNLSATSAKADQXSEAALQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2Y1</accession>
    <name>A0A1E5R2Y1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">26S proteasome regulatory subunit 7 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3887</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2Y1">
        <property type="interactions" value="1110"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2Y1"/>
    <dbReference type="InParanoid" id="A0A1E5R2Y1"/>
    <dbReference type="OrthoDB" id="1937997at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000005">
        <property type="entry name" value="26S protease regulatory subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000027">
        <property type="entry name" value="26S protease regulatory subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048723">
        <property type="entry name" value="OB_PRS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21236">
        <property type="entry name" value="OB_PRS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="10">
        <location>
            <begin position="246"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00067449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ80933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="471" mass="52511" checksum="129B8AE79BC4F360" modified="2017-01-18" version="1">MAPKEDWEKYKKPVDEETVEEKKVIPLSEDDIQVLKTYGVAPYQNRLKDLDEDIKDLEKKIKEISGVKESDTGLAATHLWDIAGDSKRLSEEHPLQVARCTKIIKDSEVKNETTESAAAASXNSAGLLGDSLLNDGADADEEDDEDAKYVINLKQIAKFVVGLGERVSPTDIEEGMRVGVDRQKYHIELPLPPRIDPSVTMMTVEEKPDITYNDVGGCKDQIEKLREVVELPLLSPERFATLGIDPPKGILLYGPPGTGKTLCARAVANRTDATFIRVIGSELVQKYVGEGARMVRELFEMARTKKASIIFFDEVDAIGGARFDDGAGGDNEVQRTMLELITQLDGFDPRGNIKVMFATNRPNTLDPALLRPGRIDRKVEFSLPDLEGRANIFKIHSKSMSVERGIRWELISRLCPNTTGAELRSVCTEAGMFAIRARRKVATEKDFLQAVEKVINGYKKFSSTSRYMQYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4Q7</accession>
    <name>A0A1E5R4Q7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein ATP11, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3815</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4Q7">
        <property type="interactions" value="317"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4Q7"/>
    <dbReference type="InParanoid" id="A0A1E5R4Q7"/>
    <dbReference type="OrthoDB" id="16535at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010591">
        <property type="entry name" value="ATP11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13126:SF0">
        <property type="entry name" value="ATP SYNTHASE MITOCHONDRIAL F1 COMPLEX ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13126">
        <property type="entry name" value="CHAPERONE ATP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06644">
        <property type="entry name" value="ATP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="191"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="193"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ81882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="345" mass="39805" checksum="9B9E5CA53420B81D" modified="2017-01-18" version="1">MFASKVANLTRIETQHLLNCSIKGKTIGLLSFTQSYSSLKENSIKEKYTNSIEEKYKQKLLERAKEQGFETIEDLKQNLQEQILEKKKQFNKVDPLKDLQEYQEKMSKNFSKDKTISKGPIDTSLEQKPFKTLNSYLQLEKIKXLSKQEIEFLWRARFANKDNTLHAVVPPMVFEPMYKNAKQNPAFVLPLPRELDPTSTDAKDNGNNKNKNKKXNNNHDEQSVGMELHYVQWNFVGPTTVHCMVTSLAEFKLHKEFARPHITMSFHTDLIKDKDLVLMNGIVEPDCNVSMQDAQLLLLNIQRFYGCMGESTPVAAKRVQLLKDFTKGSENFNIDLLIELSQSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4T2</accession>
    <name>A0A1E5R4T2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3813</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R4T2"/>
    <dbReference type="OrthoDB" id="2357318at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ81881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="530" mass="62234" checksum="E0B8D330A756A993" modified="2017-01-18" version="1">MSDENLFYVYLKRRANTSVSTTQLLDSVCYYIPKARHIEALKLYIDTLWQLEFDKNNEIELFFALHDRVRYTFQAKFEICEPFITIETFIQAWMDKIESTVDRPSLPQHLLTRLIITSAALCAVNPTLVDLEQSNVITSRNVHNFQKLLLKSFREDAFICWNFNRDLVSFMLAITPVYPGDSAINERLFLQDPKVALYVLKKFLVPFFANPNYSVTLKETSNVIQRYLSKMVSYIDTTFQKHPHPEIKQQVLQTVISHLTCTNKSLEKDYVLTLVLILNSCYEENFNDQIMEKLHSNFGLLMATTGKFSQFTELFKKCSYNEKDLHLVERLTLEFPSKNNDLFMLDYLQYARPQKDYLXLLTTYCTTKSLPKSLQEASHLAMLNMIQDSPLEIEKYLQNVILVKQQYKQLTENQLMMILNETLPKMEAMQHIANELDVQVTLLSANDPANLSKLIKFKILTVVRMLAVERLPDTVIISFLNGILKLIEKYPEKHVLLEYLWKELISNKENWYYNDVGIRWWYTRVMTSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4X2</accession>
    <name>A0A1E5R4X2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cytosolic Fe-S cluster assembly factor CFD1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3693</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NBP35-CFD1 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Required for biogenesis and export of both ribosomal subunits, which may reflect a role in assembly of the Fe/S clusters in RLI1, a protein which performs rRNA processing and ribosome export.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP2/CFD1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4X2">
        <property type="interactions" value="164"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4X2"/>
    <dbReference type="InParanoid" id="A0A1E5R4X2"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03039">
        <property type="entry name" value="NUBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028600">
        <property type="entry name" value="NUBP2/Cfd1_eukaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264:SF19">
        <property type="entry name" value="CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR NUBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264">
        <property type="entry name" value="NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ81921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="293" mass="31751" checksum="931B36A9940C886F" modified="2017-01-18" version="1">MSQDQAGPPKALDNVKHIILILSGKGGVGKSSVTTQFALSLALKGLKVGVLDIDLTGPSIPRMFGLEGKEVFQSPQGWLPIEKKFNADSNRAHTKGSIKVMSLGFLLTNRGNSVVWRGPKKTAIIKQFLSDVYWGDEGLDYLLIDTPPGTTDEHISIAEELRVIGMLEEAGLGQTQIDGGVLVTTPQSISVADVKKQINFCKTIELKILGIVENMSGFQCPYCPXCTDIFSSGGGEQLAKDLNLKFLGKVPIDPKFVEMIETQSTSTDLLEEYSSLKLYDTFKAITSAALENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4X9</accession>
    <name>A0A1E5R4X9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein KRI1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3755</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the KRI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4X9">
        <property type="interactions" value="669"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4X9"/>
    <dbReference type="InParanoid" id="A0A1E5R4X9"/>
    <dbReference type="OrthoDB" id="10252032at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018034">
        <property type="entry name" value="Kri1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024626">
        <property type="entry name" value="Kri1-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14490:SF5">
        <property type="entry name" value="PROTEIN KRI1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14490">
        <property type="entry name" value="ZINC FINGER, ZZ TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05178">
        <property type="entry name" value="Kri1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12936">
        <property type="entry name" value="Kri1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kri1-like C-terminal" evidence="3">
        <location>
            <begin position="441"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="122"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="275"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="375"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="545"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="191"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="297"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ81952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="568" mass="65916" checksum="4C7177DA4FF3C79D" modified="2017-01-18" version="1">MPRKSKSKKAQSADNAIDSAETQQVIAKHDKSDVQDDHSXSSEEEEEEEEEEDDFGDLVTEDVESGIQKVLNAIKSNDPNLLNPEVRFFDENGKVTNEALSKSHKPLYLKEYQRSRLLNGELNIGDDAEEDTQPVKTYAEEQEEDRQGILNEINKAFNNSDDESNNKDNESNNESDDEGFLQKKTKKTSLHKVEDYLPDPEKEGEEEFLKEFMDKQAWIPKKKTEKGDVVLDIDGETIENFDDKDNEDFDEAVEEFENAYNFRYEDPTSAEIVSYARTQATLRRSDTSSRRTKRDRQKQEKETQRQDLDKALNKKKREKVNKIQDIIKDLEKEYGQEIQTNPKLVEKLTNLLINEETEFDQNQWDQVLAEVFAEQEGDAAEKPTWDENDEIMADFHASTEEPVAQEDLASSTASNKKSKRKAKLEAKREGKKQKKDLQNLIENAVDDNKLILLDEVEEERKSRSXNKDTDSGLKFKYREVSPDSFGLTYREIFAADDEALNKFINIKKFAPYRPKNLRDKDRRKVTKSKRMAEWRKEVFDNKNGLAPYDMETQESTKHKKQKSKESDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4Y8</accession>
    <name>A0A1E5R4Y8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3742</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4Y8"/>
    <dbReference type="InParanoid" id="A0A1E5R4Y8"/>
    <dbReference type="OrthoDB" id="18087at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005120">
        <property type="entry name" value="UPF3_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03467">
        <property type="entry name" value="Smg4_UPF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF3" evidence="2">
        <location>
            <begin position="282"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="479"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="126"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="479"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="496"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="529"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ81977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="579" mass="65577" checksum="44907783B5D7EC4C" modified="2017-01-18" version="1">MSSTEKEHQPATANSNEQMNTQHENNKTKGKSKNNNKNYKKKQLNKSDAKKDPKTGGKMTNKNQKEEHKPAQEGSKQQGFETEXGAKQIANQKQKTNKAQKHQSNNMQKKNQEQEDGKQQKKKNQQQKQNINQIPTQKPVGEGETKTNVLGGLHSDESNVGKKAKVSNTFVNAKTSFKSQKYDNAKNSQSNIDGRAKEGADQPHSDRYDKHVNNQTKDTIKAKGLKKTGPTPARLNKSTKKEEKQDGKEADLSNSNVSIITKKDKKKRVRNPRNRKEKVQLGTRLVVRRLPPDLKEEEFLSDLPEDLKSVFQYFKQGVHNFNNPFEEPVLSRAYFQFPFTKEPENIALAKYLASANYVLAKYKPNAKLEKCTFKYRSGNPLDLKRPNRVTNTLKNDPLYKMFQKNWDAHFAQKKSETDTNSSIDTAQSFAAKNVDHVSLSNVSLFSKDMLKAAESANRESNKHFTFDILAKEKNKKVDVVLSEHKKGTTNKERSKKEKKSKKSKKSKKSKSSDSTDASMQKGSDDAKRTSRKRKDKAKKKKKKNSKHAAKTMAPTTHSSEGGRAVFEAAGLKLLSLRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4Z9</accession>
    <name>A0A1E5R4Z9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3647</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4Z9">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R4Z9"/>
    <dbReference type="OrthoDB" id="1932312at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ81969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="585" mass="66948" checksum="F8D29C78BE1C0BCF" modified="2017-01-18" version="1">MDANTGNTDQGVLQNDHKKLVNLLVAKYLIKQGYKETFKKFIDESDSIDFLEFXTNEIDGIDLNKNEEIGLNGNDSEKNVVKENLETLIYERLEFNEFIDKSERAKPVIRTNITTTEEEEQEQRVKDSIKKLLSRNYDMPKLHESSLHRILLKDGNSIEHALMSRLCTGKNYNMRIIKLVVCGPYLLCSTSSKHLVVFDIKKDYKLIKTFQFNSIIKFISSKLIVLKPKDSKSNTEQKNYIYCCGIDGKFNLISCDTWDVVIKDHVLSTSRAISHLEIYQMSSCNPCDNPCDNPCDNPCDNPFATGKHLINIVTCGMNKHVYISQLDMNSLEFKNMGTTHTPMLSGITNMRIFQSEVGSATVXNICXKTYMALSLVDNTQLFIYEIPXTKENTEITLIAKIALNNAQFSTHSFNISNFFIFNAQNDTEIGIKAKRQDWKILITTSHIPYMRVLVVDLEEKVFVANPQISTTVPIYYDLVKVNFVTDIEQSNYSSSDILYNRVYKNFSIVTQFCVYRVIGFKTFQILREFATTEEKKKTNINNIQHSKADHTLKTITSCSITDDSKGLIISGTSDQNSEPIIRLYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R509</accession>
    <name>A0A1E5R509_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DSC E3 ubiquitin ligase complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3812</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R509">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R509"/>
    <dbReference type="InParanoid" id="A0A1E5R509"/>
    <dbReference type="OrthoDB" id="272778at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="300"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="300"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="315"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ81995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="344" mass="39654" checksum="FECF5F10AFC491D9" modified="2017-01-18" version="1">MLMKSYPVTRWLILFNIIIPLLFNVIVPGFQNYFLIRSAAPYITEYHQYYRYVTFQLFSSGLESDICLLVILLYTFKQLERAMGKNQYLAVIAGLFFYNMLITGVLVHYGLVVDVFLQKFNFKEKYGYAFNDDYGIGLPSGLTCCIVGLFHFYKKYVPSKFTFSFQLKSKTKPEQTQTIVPGERILGSNESQDLGSTVQSKPQTINDDLERVQPSKLSKTSQNQGIVFGDQFFVNIMFLLLILNNLQSLPFFMISWGIAVLFDTGLLPGKKFRIPNFLLFNYNRRNYTMLSSYNSIMSRRSGNPHXASSFSNARDDDDDDEDEDEENTEPVRPLAVQIMNTFHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R593</accession>
    <name>A0A1E5R593_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Mitochondrial transcription factor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3711</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="OrthoDB" id="2444174at2759"/>
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    </dbReference>
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        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Mtf2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040009">
        <property type="entry name" value="Mtf2/C5D6.12-like"/>
    </dbReference>
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        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39468:SF1">
        <property type="entry name" value="MTF2-LIKE C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="Mtf2"/>
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    </dbReference>
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    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="247"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="434"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="83"/>
            <end position="110"/>
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    </feature>
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        <location>
            <begin position="441"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF19189"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="OEJ82038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="491" mass="55896" checksum="C0AA0E241D9B7D03" modified="2017-01-18" version="1">MQRTIQRATXLRSYNASQSFKRLFSRTKTLQNEAFDHAKSLKKRTAPSDLLAQAPLDEEHSTLVVGREHRVFQDAIEEFSNTSEQKENSYEQSLSEIAGYENEIKQAIAEDGNSAFDAAFPSPNTENSSLFTETTPSNSDSXNQVEHLRDLFDPLTASAPSQSIFQRKPDLKQIKKYDINLSSYLKEDGEKNAVNRFEAVLAKKPRSSNADLSVPSLQTDMNGDSELIDEGMLKYKEGIIKKKQQEEELEQLLQPVLDNIDKSIEDDNDLLTVISKYIISLRKYLSQDHSLDQSRRGDNLRTIPDPLSPDLSIPQPVQINVPFIIKHLLMCEKYDFVGQDRKYVILSWVHNVCKNYHKDINLYLSVFTIDLYNYLLDLTWMEFKNISSVFLIVTEMRANGVKGDIETIKILEKIDLEIERNQMLLSARFAEAPVSAPPSPRFGGPXGSDFSESGTPSSQAVRGMRLIRSMAQVQQRSKLQHHISMLKNMLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7A8</accession>
    <name>A0A1E5R7A8_9ASCO</name>
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        <submittedName>
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        </submittedName>
    </protein>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
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            <taxon>Saccharomycetes</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
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                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        </location>
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        </location>
    </feature>
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            <begin position="512"/>
            <end position="575"/>
        </location>
    </feature>
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            <begin position="715"/>
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        </location>
    </feature>
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        </location>
    </feature>
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            <begin position="960"/>
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            <begin position="1121"/>
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    </feature>
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            <begin position="200"/>
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        </location>
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    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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            <begin position="724"/>
            <end position="734"/>
        </location>
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    </feature>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1325" mass="146164" checksum="C1F2DC242E0C6BF4" modified="2017-01-18" version="1">MTESSNNLNFVKNGSKKTLMKRYTSPFDRGSKKKQISTCIQRSLYCVSKPWNDGHSSSLVFFPKTRDESKLSNAVPLLSENIEPLHNAVSWMEDLIKQGKQALGEVTTLEKEFVDSVNTIQLKELAHKSLLEKSLQALSSTEKCRIDDLDNKSKKSYVETAGGSLSQDFGLEENQTDERLSGSNDDDEDMDNRVNKPLAQEEEEEEEEEEDDDDDDEEEEAEEDNVVIMIDSSEEESTQDLLNSPAKSFEDTFEGSHVQTAIQDPSHKISSDYMQNKNDIKTTYDKVESPRIKKRIGPIITDSELEVSENEIPEPYLSFEEQSEEYSEQSFYESDGSNEQKNEVPETLDNLQFQKTERASVGGKYPGKFPSVNHDQSLKESAAESFDDMNALHKEQKFEYAQVSESSELEEEEEEEEEEEEEEEEEEEEESAELFEHSDSERGQLKQTVYYHLEPLSKKENNGQLTTATSFVAHDNDELKVEHGNNVGQSAPFDSINSYTAKQDPTYDANFYSTPSFSTQNAPALMDDFAQNKADFQDGESSRTQSVFEISDSDESRTASRPSENVADEPMDDSNLTDLATSALLSMKMPVYEAYDRILPEKNKESQQSFIDPAFNELSAEISNTAHELNDSSQTHTEQSYASAGSQNLHDLSNNVFASDDFVNAESLSKIETALNIEHENEGGTSNGKLNDIESTESEPFVKRVEEITNIDLFENPSDKRGASSFTNTDTNNPSADTMALTLQAHEANETDSMSAHKLADSQTTTSQSPFPTFPPFENADGYKKNDENMKEASVSAQRNAEYHLVYETSPVSSNNSTPKEVLIPSAHAGVFQTNPFDQTNVSVDLDVILATIQENISSSNANENVYVSPVNDLEPPTLEEPAENYQVSAKPDLNADTEISQSNGEGTPNKNDALGGLTAIEKNKMNDLLLTNHKDSEPVNDPVVVNKSESSLLNIESMAKQQNSSTTTEQNENAEKHASNANVVDKAFVARENHGAKSESDIGGLPTDNLISPLLAEQNNSTVDATETTLGHPAFQSVAVDVAQSKTQISKSKEGFVQSEDVFDGTVNDAVLGPEVVTDLLALHNDSLDHFANIDSAQAPVAETVTEKVDAAPGKSFLEIADPDVTELQVETDSRETNISNATSKENSNENSEYQERDINENNLSSSEKQEIQHSINDKNVQELPLDEDTTSDSFPHDKAAVHDESIQTKGLDQSPPSAEDLAGTVKDDKATAKASEVGSSSHLDHSSLAGNQASLDDGIAEKSFEAGKEAPSAGPSPKVPKSPKSSKKKKRAVRTKKRMIIEDHSSAPISKRLRSGNQKSKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7P6</accession>
    <name>A0A1E5R7P6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sterol uptake control protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3181</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ascomycota</taxon>
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    </organism>
    <reference evidence="4" key="1">
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            <authorList>
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                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784:SF5">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
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    </dbReference>
    <proteinExistence type="predicted"/>
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        </location>
    </feature>
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        </location>
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        </location>
    </feature>
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        </location>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="697"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="985" mass="107970" checksum="54B64753B9E8B327" modified="2017-01-18" version="1">MAEQNFMDPLSTDERTSSNEIDLIELNGKQVSKNSTGKRKFHHKSKEGCDNCKKRRVKCSMEKPHCHKCVHMKLDCVYTPVQPRKKKPRVSKKPVSTTIDSIKSESDQSLNADQTTQNQKIENSDGNEKKGNVKDTVKRTVKRQRASKKDSQKTPKINGSGTTVKQESPAGPVLDPLKTDLNNNFQIAHQNDPSGLLLPPLMPPNTLQQQIQQQIQQQIQQQLQHLQPHLLPQLMTLSNSEKYMTNDSPFSNVLNSPQSTGMNLTNSIGTFFSLNNAKMLSNLTAAPGMKSGGNDTVNHNETHLTNNTGITQNSQKKKLKKESSSSNMLKKGIPSASHTHSSVAAMPNKMPSTPGIFQPAGIGGITYDFHELLTSKIPGASKNDTLSSNLHDNENATASHTINSSNEHNLGPTNDDTNVFTHLTRTPSPSSRNQHSQMDTMISGPQSMGRQGSPRQQHFHSHHTHHEQSHDETVRQQTDPISKYQQDAKDEAVEVLSTMASVAQMDKQMSSMNNYTKRPNKKPLISHLLAIQSQSGSGNLNLVDLKLFQHYCNKVCGTITDAGISDHDIWFDEIPNLAFQYPFLMHSLLAFSATHLARTEIGLEGIVATHRLDALRLLREAVLQITPENTDALVASAIILIMDSLANAASPESNEDDYEQKSSNVNMSVAIDHHGNQEVDSNASKAEDVVNGLDHNSSSASSTAPSSSSHSDGPGDAMSAAAAAASANGIASSMARAAAAGASSIANLSTSAWIFHVKGAATILTAVWPLPETSRFYNLINVDLSELGNLITNSAVVSPQHLHTDMNDVDIRSAHSQGQAEHGSMENFANAGHGSDSRKRDMIHSTITDLICFDDSIADLYPVELDSPYLITLAYLDKLNKDRNKSGFILRIFAFPALLDKTFLALLMTGDLNAMRIMRCYYKMLRGFASEYKDKIWFLEGITQVLPVDVDEYSGGGVYMMLDFLGNGLPSMTTTNFDSQQPWAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7R3</accession>
    <name>A0A1E5R7R3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribonucleases P/MRP protein subunit POP7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3340</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7R3">
        <property type="interactions" value="94"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7R3"/>
    <dbReference type="InParanoid" id="A0A1E5R7R3"/>
    <dbReference type="OrthoDB" id="5416589at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000171">
        <property type="term" value="F:ribonuclease MRP activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034965">
        <property type="term" value="P:intronic box C/D snoRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000294">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, RNase MRP-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014612">
        <property type="entry name" value="Pop7/Rpp20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020241">
        <property type="entry name" value="RNase_P/MRP_Pop7_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28256">
        <property type="entry name" value="RIBONUCLEASES P/MRP PROTEIN SUBUNIT POP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28256:SF1">
        <property type="entry name" value="RIBONUCLEASES P_MRP PROTEIN SUBUNIT POP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12328">
        <property type="entry name" value="Rpp20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="AlbA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="108"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ82952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="142" mass="16216" checksum="9BC1C010F4377F1E" modified="2017-01-18" version="1">MSKSYNTRSKNIIHXPTAKPVSYNEIHTKFYVKPNASFIATVKQIKKMLKNCEKKNLKDHISVVGMGRAVGKAISVSSHFSENHKTQVFTKTVTVLDEKITSTKTEKNEEEDEDFENDQELNASQLEKRSVSGIEIRIYIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R944</accession>
    <name>A0A1E5R944_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Oxysterol-binding 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3404</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the OSBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R944"/>
    <dbReference type="FunCoup" id="A0A1E5R944">
        <property type="interactions" value="111"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R944"/>
    <dbReference type="InParanoid" id="A0A1E5R944"/>
    <dbReference type="OrthoDB" id="14833at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008142">
        <property type="term" value="F:oxysterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120015">
        <property type="term" value="F:sterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030011">
        <property type="term" value="P:maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF184">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN HOMOLOG 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="282"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ83053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="481" mass="54207" checksum="3C385B6DEE41C397" modified="2017-01-18" version="1">MGESSKSSSTANSSSWTSFLKSIANFNGDLSSLTAPPFILSPVSLTEYSQYWAEHPDLLLSINNAVNKNTFEPSFPSVVEYTPDLVKMLHVVRWFISTLKSQYCSRNESMGTEKKPLNPFLGELFVGKWEHEQNGSVTADLGDTVCLSEQVSHHPPITAYSIFNEKTNVSLEGYNQIKSTFSSASLSVKQYGHALLRCKNKEQQEQKDDTFLITLPSLHIEGLLLAAPFVELDSKSYIQSSSGYFAIIEYSGRGYFSGKKNSFKCKIYKSYESYIYDKEGKPSSSASQEASGSSSEKQNNKEDKKPLFVISGQWSGISNIYTSSSDEKKRKNGQVFYDASRVAAEHLHVKPIEKQNPWESRRAWEKVANAIKSGDYNLIHQEKSQLEEEQRMLRLSEQEKNTTWKTRWFDNSSINSVQNEDFVSLAKMCNLSVKNVPSGTRVGDKYDTHASASTDGSNGDEVVKHWRFVKENWANETEITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R9A8</accession>
    <name>A0A1E5R9A8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NAD-dependent histone deacetylase HST4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3158</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R9A8">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R9A8"/>
    <dbReference type="InParanoid" id="A0A1E5R9A8"/>
    <dbReference type="OrthoDB" id="2919105at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031934">
        <property type="term" value="C:mating-type region heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017136">
        <property type="term" value="F:histone deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031508">
        <property type="term" value="P:pericentric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006282">
        <property type="term" value="P:regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF15">
        <property type="entry name" value="NAD-DEPENDENT HISTONE DEACETYLASE HST4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="7">
        <location>
            <begin position="234"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="55"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="112"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="113"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="134"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="382"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="393"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="434"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="437"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ83123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="564" mass="63222" checksum="3BF0A2EEBB815A05" modified="2017-01-18" version="1">MPSLTRRTQSTGSLLLKNKCLQDPRIAQPHQLRRSPRRHRHENVVLSPDXTHCEKKXXLSLSPGEQNGYMSPPLTDVKRQNSISKKNGSAKLGSNXRQKDAKKRLLAELLQLASPTISTPQTPTKKNSKHTVPSFLSSSSTLSTLSTSSTSSSSSSSLMSLSANSTPFGSPIKRRKPKLKYRPASNSVFFLDKYIEEVLRMDVDTADDXDDDLVEQLSKTPKTSRKXSRNNSITDEENQDIQEKAYFLKYSLLNSKKMVFVTGAGVSVAAGIPDFRSTAGLFQSLKKENKVSNGKDLFDFNHVYSNQEETVKFNKMVCSMHQAIQNSTPTQFHSLLNWLGNEQRLKRVYTQNIDGFELRLDNLHTESPLSGKKPFPNVVQLHGSINHMSCFKCHKVFDFDPNIFGTSEDLIEYNEETGEILETQENDHKIVPLCPNCKELEELRVIAGKRSQGIGKLRPRVILYNEFHPDGEQIGDIVSQDISSSPDCLIIAGTTLKIPGVRRMCKEFAKKIHSKNGIVLWINNEVPSKAIQDFLEFIDLIVVGDCQNLSSLLIKDASFGNLQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA32</accession>
    <name>A0A1E5RA32_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA-directed RNA polymerase III subunit RPC5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RA32">
        <property type="interactions" value="149"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA32"/>
    <dbReference type="InParanoid" id="A0A1E5RA32"/>
    <dbReference type="OrthoDB" id="340681at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006886">
        <property type="entry name" value="RNA_pol_III_Rpc5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12069:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12069">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES III 80 KDA POLYPEPTIDE RNA POLYMERASE III SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04801">
        <property type="entry name" value="RPC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="282" mass="31468" checksum="D5450151E3EC8E30" modified="2017-01-18" version="1">MSVKQENINDDTMIDTEIIQNEANIASTVLENEAMDIDGSAXADSAVQEDESDAEDYDEEDDPVVKSIPINLNTLAYPINVFQYPTKPKKLLGTRTELPHPSIAAVRLKPNSKQWEMEVPLEQNVFFDKEGSEEKWPNANIENQVLTSVTVSENGQYVAFYRDEQLYLLPVESFGQFRPLFKHIDSQNGAVSATNAPTNGANSSGTGKTTVVTMSVKSSAEANQNRLGGALLAQKISEEETFKDLQWVDHTFEQFMETVMTEESKKKVENASSKEDYFAKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAC1</accession>
    <name>A0A1E5RAC1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2954</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAC1">
        <property type="interactions" value="173"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAC1"/>
    <dbReference type="InParanoid" id="A0A1E5RAC1"/>
    <dbReference type="OrthoDB" id="448399at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1060.10:FF:000024">
        <property type="entry name" value="RNA-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23111:SF40">
        <property type="entry name" value="RNA-BINDING PROTEIN INVOLVED IN HETEROCHROMATIN ASSEMBLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23111">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="6">
        <location>
            <begin position="465"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="6">
        <location>
            <begin position="742"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="239"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="517"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="239"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="517"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="536"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="7">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ83844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="770" mass="85319" checksum="00BE80E5E5AB0F66" modified="2017-01-18" version="1">MKFLVLEIVTSMATLPDATSIEYQPLRQDMNIVKIFWKMLDPAEILNKNNGQITSQMAIPYNRIEINDLVPLPQAIQNLDMAVKVEMSKCNEQNNVAKNNSGELDKSFQQSSHDQMPIIISLHSTWDIRVLLSYKAKSLNFPLPTWLQHPTIFDLCKEYERWCIKDATRYNIFNAAALNINKRRKLHSNKIYLFTLLQLLGLKMEMYEMDEFGCIIKMFLSLYLKSGALEAAKASSILSPSESNSSASSSKKQKEDALENTETETDSPSFTLLDKSAESTTRENAGFTTPYDILLDYNFFLHEESKVVYLNNLPSIVTQSELEKWFYNNGCHPIGFWTVKPAINAPLNYSTDKISFTYVPDSDTISGYAVFQSHAEALAGLELNGKSLVLRLGSNNNNNNNHNNINNNHNNTAHSNHSANAFNHQSKSRYKYIDRVVEVQPSSNIVLTKIRNLLVPFPQTRNKPRPGDWNCQSCGFSNFQKRTTCFRCSHPHFINKNSNSMMNDDNAGNSFGNXKNNNIGQNTSHGSYLTGDSKSNGTYMNNGNNGNTTEPNGERKFMVSENLQRGNSMNTGSDSFYANVGNKNGTISGXAAFANTVSGAALRTSLYASPAANSSSNVSSASSASNVGFTNLNYXXNQMTGDSVDTSYALKKNFTSDFPTNDTQNRVEYGMSSNNFAPNTSAPGIYLNTSNNASGISVGSTTTTNGGPSLNMLGSISSSPANMNFKSQLSTDSAAQQDIPFRPGDWKCTNCAYHNFAKNMNCLRCNDAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAE3</accession>
    <name>A0A1E5RAE3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pre-mRNA-splicing factor CWC26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2843</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CWC26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAE3">
        <property type="interactions" value="78"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAE3"/>
    <dbReference type="InParanoid" id="A0A1E5RAE3"/>
    <dbReference type="OrthoDB" id="6022at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070274">
        <property type="term" value="C:RES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018609">
        <property type="entry name" value="Bud13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051112">
        <property type="entry name" value="CWC26_splicing_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31809">
        <property type="entry name" value="BUD13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31809:SF0">
        <property type="entry name" value="BUD13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09736">
        <property type="entry name" value="Bud13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="72"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="109"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ83868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="296" mass="33341" checksum="7C767C768BE2634E" modified="2017-01-18" version="1">MSGKRSLQDFLSSQYGSKKSNSKPLKTPRPGSLSIIDESASALSASRTQLISKKNGRDGDRMKKXKSFAFKNLSSNEVSTINPSDQNIIAEENLPSGXPTVDHSASKVAGNQKTESKETQNTSTSXAKGSPSASYPQETIYRDEKGRKIKNYREYMQTKQSXEQRNKLIEEQAVIELNKGEIQNAGLEXAASFSQSSKRKRETTFHSEDPLAMMASSNIENRKESVIVERVNTSASTTPLGRRVYEGITPLNRFNIQPGWRWDGVDRSNMFEQKWFAKKNEIKEKRVHEYSMRTDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAF7</accession>
    <name>A0A1E5RAF7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Protein-S-isoprenylcysteine O-methyltransferase</fullName>
            <ecNumber evidence="3 10">2.1.1.100</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2624</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine + S-adenosyl-L-methionine = [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:21672"/>
            <dbReference type="Rhea" id="RHEA-COMP:12125"/>
            <dbReference type="Rhea" id="RHEA-COMP:12126"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90510"/>
            <dbReference type="ChEBI" id="CHEBI:90511"/>
            <dbReference type="EC" id="2.1.1.100"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.100" evidence="3 10"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAF7"/>
    <dbReference type="FunCoup" id="A0A1E5RAF7">
        <property type="interactions" value="474"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAF7"/>
    <dbReference type="InParanoid" id="A0A1E5RAF7"/>
    <dbReference type="OrthoDB" id="422086at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004671">
        <property type="term" value="F:protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007269">
        <property type="entry name" value="ICMT_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025770">
        <property type="entry name" value="PPMT_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12714">
        <property type="entry name" value="PROTEIN-S ISOPRENYLCYSTEINE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12714:SF9">
        <property type="entry name" value="PROTEIN-S-ISOPRENYLCYSTEINE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04140">
        <property type="entry name" value="ICMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51564">
        <property type="entry name" value="SAM_ICMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="180"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ83882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="242" mass="27594" checksum="E9221E8F1B856E3F" modified="2017-01-18" version="1">MNDNESVTSSKTDYPYPDIKENPLDEISITSYILGTVLGISVPWLINAKFVGFPLYLISLSAFHFLEFYVTAKFNPLKVDSNSFLLRNGSGYSAAQLLAICECLIEMQFFSFKSLSTFHTKLIFGLGVCLLVVGQVARSTAMITCGRSFSHRIMQEKQDDHQLVTKGIYQYSRHPSYFGFFWWAVGTQLICLNPISFIVFTVVLWSFFKKRIQHEEQLLIKFFGKEYLDFKETVGVGIPGIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAI3</accession>
    <name>A0A1E5RAI3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PITH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2572</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PITHD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAI3"/>
    <dbReference type="STRING" id="56408.A0A1E5RAI3"/>
    <dbReference type="InParanoid" id="A0A1E5RAI3"/>
    <dbReference type="OrthoDB" id="2635at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.470">
        <property type="entry name" value="PITH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045099">
        <property type="entry name" value="PITH1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010400">
        <property type="entry name" value="PITH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037047">
        <property type="entry name" value="PITH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12175">
        <property type="entry name" value="AD039 HT014 THIOREDOXIN FAMILY TRP26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12175:SF1">
        <property type="entry name" value="PITH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06201">
        <property type="entry name" value="PITH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51532">
        <property type="entry name" value="PITH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PITH" evidence="2">
        <location>
            <begin position="11"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ83916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="218" mass="24618" checksum="CF555D9799B4F63A" modified="2017-01-18" version="1">MPCTDHDHTAPPALPTSEQQNLLPYIDKTSFKVLNGVFKPLGDVAAGAAYILEDPTGDKYLESDCDCQLLIQVHFLQPVKLYSLLVDTTNSEDTIKHIHLYKNVEGHGTNLTFDKIQATKFDFSIECRADGNTDLQEYHVPRPFFKNVKSLVLFIPDNLEEDEDLLSKINYLEFRGDPVHSSQYQAQEFSLRGQVFESAPNLNDHLKLDDGSSSKMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAQ6</accession>
    <name>A0A1E5RAQ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">homocitrate synthase</fullName>
            <ecNumber evidence="6">2.3.3.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g2908</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the aldol-type condensation of 2-oxoglutarate with acetyl-CoA to yield homocitrate. Carries out the first step of the alpha-aminoadipate (AAA) lysine biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>acetyl-CoA + 2-oxoglutarate + H2O = (2R)-homocitrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12929"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58884"/>
            <dbReference type="EC" id="2.3.3.14"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:12930"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-alpha-aminoadipate from 2-oxoglutarate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the alpha-IPM synthase/homocitrate synthase family. Homocitrate synthase LYS20/LYS21 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.14" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAQ6"/>
    <dbReference type="FunCoup" id="A0A1E5RAQ6">
        <property type="interactions" value="667"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAQ6"/>
    <dbReference type="InParanoid" id="A0A1E5RAQ6"/>
    <dbReference type="OrthoDB" id="2015253at2759"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00028"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004410">
        <property type="term" value="F:homocitrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07948">
        <property type="entry name" value="DRE_TIM_HCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.260:FF:000002">
        <property type="entry name" value="Homocitrate synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000032">
        <property type="entry name" value="Homocitrate synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02222">
        <property type="entry name" value="Homocitr_synth_fung_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050073">
        <property type="entry name" value="2-IPM_HCS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048253">
        <property type="entry name" value="DRE_TIM_HCS_fun_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011872">
        <property type="entry name" value="Homocitrate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054691">
        <property type="entry name" value="LeuA/HCS_post-cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02146">
        <property type="entry name" value="LysS_fung_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10277:SF48">
        <property type="entry name" value="HOMOCITRATE SYNTHASE, CYTOSOLIC ISOZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10277">
        <property type="entry name" value="HOMOCITRATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22617">
        <property type="entry name" value="HCS_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="15">
        <location>
            <begin position="38"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ83977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="441" mass="48603" checksum="1725D32109BC2D50" modified="2017-01-18" version="1">MSDSFQKVTEASGTGSASVFSNPYGPNPSDFLSNVGNFQIIESTLREGEQFANAFFTTEKKIEIAKALDDFGVDYIELTSPVASEQSRADCEAICKLGLKAKILTHIRCHMDDARVAVETGVDGVDVVIGTSSFLRQYSHGKDMNYISKQAVEVIEFVKSKGLEIRFSSEDSFRSDLVDLLNIYKTVDKIGVNRIGIADTVGCANPRQVYELVRTLKSVISSSTDIECHFHDDTGCAVANAYTALEGGARLIDTCVLGIGERNGIVPLGALMARMIVSAPDYVKSKYNLKKLRDIETLVAEAVQVNIPFNNPITGFCAFTHKAGIHAKAILANPSTYEILNPHDFGMTRYIHFANRLTGWNAIKSRVDQLDLHLTDDECKEVTAKIKKLGDIRQLNIDDVDSIIKDYHVEVNTPAVKVVNKDNKDIDELDINEPAAKKQRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAT1</accession>
    <name>A0A1E5RAT1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Telomerase reverse transcriptase</fullName>
            <ecNumber evidence="2 13">2.7.7.49</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Telomerase catalytic subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g2593</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.49"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Chromosome</location>
            <location evidence="13">Telomere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the reverse transcriptase family. Telomerase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.49" evidence="2 13"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAT1">
        <property type="interactions" value="311"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAT1"/>
    <dbReference type="InParanoid" id="A0A1E5RAT1"/>
    <dbReference type="OrthoDB" id="289721at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000333">
        <property type="term" value="C:telomerase catalytic core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003720">
        <property type="term" value="F:telomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070034">
        <property type="term" value="F:telomerase RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01648">
        <property type="entry name" value="TERT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021891">
        <property type="entry name" value="Telomerase_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003545">
        <property type="entry name" value="Telomerase_RT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12066">
        <property type="entry name" value="TELOMERASE REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12066:SF0">
        <property type="entry name" value="TELOMERASE REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12009">
        <property type="entry name" value="Telomerase_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01365">
        <property type="entry name" value="TELOMERASERT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00975">
        <property type="entry name" value="Telomerase_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13 15" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="9 13" id="KW-0779">Telomere</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="14">
        <location>
            <begin position="462"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="15">
        <location>
            <position position="809"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ84002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="991" mass="115877" checksum="1F521A675A4F184C" modified="2017-01-18" version="1">MRTKTRNINANKSFQLKTLYEHVEELLNASNTGTNNGINELDIFLHYHSDFLFKLFVLKENDTKKETCISTLTPPPGLENAFIGQVVVYQESLPIDLNXHLEIINYVIAVLKSMQYGTNILISSPSFIINNTEWFILHQVLGSWNFIDLLLNYKIFEYMEENLFTQLSGNRNLINNHNSIYVNPYKKVVTEDGPIGMKIFSPQYYLFKLQNAALQVSITNDTENTLIEDKTKELKKMXYNLIPNESILQSMNIIFXNNFNENSPLYLSFKPVWSLVKKNHKKLAYWNILNTVCPLLKNKEPPTTINSAIDVGKVIKFLLIIVEKLFPLELWGEKHNKAIVFHNLNKLLKMDINKSIPMHLLLQEISFNKMTWIGQTSSKDNPNFRKEGERFFEWFYLKFFPKXVKTFFYVTNISSKITQIVYYRQDXYMKIVYPFISKYCDDNLQKNKTCVLLPNDIQTKNQNQNXIMHDLSCLHTTLRIVPKNLKLPNDLRFISIPSPHIQFKDLMKFKWFVQDHIIPVRKILQHIRLXYLSVXDSFPKLSNLYEIPILIKNFKNLLKVKDSVYFLKFDVKSCYDSIPIDLFITLLQEKWLKDEKLLNKTNNGQWIVYKNSVSLLNTNLAFKQVVRKEYIINGVNALHNCTRPNNSTIQGQNTVKEKDRNGISDFIKINQRNTDIQSFTNEDILYVVKKELLQTCVTIGDACFQRKIGVFQGTFLSSIIMDLLYDDMLRSTAEFHSPSKQCKLLVLRLADDFLVLSNSYEKIQQIMERSTRGFPEYNAYINSEKLEFSHCKDVSKNPANVNFVGLTVDIPSLEMSKDSETFTNIKYSFEPSLKAVYGKLLNLIEIRLKYNLLNIQLNSNQIIIRHLTLIVSNLVQSWIEFVVLXRTKLRLFDNLQTKTGMQNFAKFIKNLVKSLHYHLWKINSYIFDQKNSKLAKRKQFKKLKSLSFRYNALEKNLSAIVTQTIIAKLHENQKTKTNLGKLIDFVQQMRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAW3</accession>
    <name>A0A1E5RAW3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Dual specificity protein kinase KNS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2840</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAW3">
        <property type="interactions" value="746"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAW3"/>
    <dbReference type="InParanoid" id="A0A1E5RAW3"/>
    <dbReference type="OrthoDB" id="3971676at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051175">
        <property type="entry name" value="CLK_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE DOA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646:SF11">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="271"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="75"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="817"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="35"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="82"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="817"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="827"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="863"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="898"/>
            <end position="914"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ84047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="968" mass="109261" checksum="AF325542E987BE72" modified="2017-01-18" version="1">MNHNSNQGNQSVSGTSTGVRFFNRLLSRQKDKNVSGKNDNTNNTNVQRNINGMGIINEINNENGNNDYDYDYEYGYDKDMMNDQDDDQDDVDSENTSNDNGDDDDVIFIKEVPVTNNLKTNSNINNTYSNAKYPNYITNTKKRKVSVTEQPMFKKNTLNQLRSQANVGNGYHIAQNNNVFQNSPNKNTTINYNASPNINSSDNQKYNNKYEKDLFDEEENSDEDLSEEYDDSNDEVSSEMFQDVIEDDKFKTDADGYYIFEPNKYIYNKRYITQKLLGGGTFSKVILVYDTKEHKQKAIKIIKSIDRYRMAAKNEIKVLNALSNCIXRDLHPVVGKSLSQYGQSSDYPILQLLDHFDIKNHICIVTDSFPCSVYDFMSSNATPSFPGSQLQHMGQQLIESIVMLHSLGIIHTDLKPENMLLESIKCGAEYQLSLQESHLLSSRRLKASENGKRKILSNTNVKLIDFGSAVFAHEFHPNIISTRHYRAPEVILGYKWSFPCDIWSIGCVLYELCTGETLFPIHDNEKHLIMIQKLVNENIQLDPKLVIQNGSLSANXNNINNITNSITSNPSGSRWNGLGKTDLTNAQMLKSINPNTKEVIHMFDPQNDYKFDWQXFNNGKNTGANTGGIGMGKITKTKLKTGSKQRKSLYQTDLQQVLMTCDRLDKLLTVKLKKKLPWLELVNYEMNIVDNWAYIQSLHPEIKNNSFTETSCKATVTSTFENKSYTTNDGSSIQQLSFDVFKFWYLFVDLLRVMLAVDPLKRWTAKQLLEHPWFSCVALDEGTISREKYLALHHSEATKSTTPTEYXLTNTTTHDTLKTITDKNPHNDTIGSDNSLLAPQYNHPSISSGTGPSTAGSNLRQKNYPXYPNYNSSGSSGSSTESNAGNTNYTSKPFHSTGLNNNVLNGGGSNAATNNTLGNHRMNVSVLQALQASTGQTNMQNVDQRHISNESESTTNLTNRLNNLNTTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAY5</accession>
    <name>A0A1E5RAY5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Golgi to ER traffic protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">GET2</name>
        <name evidence="11" type="ORF">AWRI3579_g2929</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Together with GET1, acts as a membrane receptor for soluble GET3, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. The GET complex cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Golgi to ER traffic (GET) complex, which is composed of GET1, GET2 and GET3. Within the complex, GET1 and GET2 form a heterotetramer which is stabilized by phosphatidylinositol binding and which binds to the GET3 homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Golgi apparatus membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the GET2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAY5"/>
    <dbReference type="FunCoup" id="A0A1E5RAY5">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAY5"/>
    <dbReference type="InParanoid" id="A0A1E5RAY5"/>
    <dbReference type="OrthoDB" id="4097053at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043529">
        <property type="term" value="C:GET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045048">
        <property type="term" value="P:protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03114">
        <property type="entry name" value="Get2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014802">
        <property type="entry name" value="GET2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028143">
        <property type="entry name" value="Get2/sif1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28263">
        <property type="entry name" value="GOLGI TO ER TRAFFIC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28263:SF1">
        <property type="entry name" value="GOLGI TO ER TRAFFIC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08690">
        <property type="entry name" value="GET2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6 8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 8" id="KW-0813">Transport</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="8">
        <location>
            <begin position="1"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="8">
        <location>
            <begin position="325"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="110"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="40"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ84059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="329" mass="36057" checksum="1A8428EBD533D765" modified="2017-01-18" version="1">MSAELSAAEKQKILRERRKQKFAKVGGSGRLNKIVGKSDSLLSTESPLDQKASLKTSSSGDNSASTTEMEQLINNVHNTPKTSNSTKNEPEIDPALKAQLDIFKQFAQQQQQEEQFAGAPGLNGSATGAGAGASGLPEMPDLFSQLAKGFPGADGTGAGSGADIFGGANPFMDAAPMVPPEVVQYHTSTLNQFKARNTFIKWVFILLPYMYFVTHPEFLATLSTENTFLIQLFSKNNFFTVLTTLEIISISLYYQFKQKLSKKTKVTATSESKILGLLKMIPEGIIPIRNLHGKVNLVFQYLDVFNLFVVDLCFCLIVMGIMTYVHNLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAZ9</accession>
    <name>A0A1E5RAZ9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcription factor VHR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2735</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAZ9">
        <property type="interactions" value="164"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAZ9"/>
    <dbReference type="InParanoid" id="A0A1E5RAZ9"/>
    <dbReference type="OrthoDB" id="4089008at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007147">
        <property type="entry name" value="TF_Vhr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04001">
        <property type="entry name" value="Vhr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="717"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="118"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="717"/>
            <end position="726"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="757" mass="83789" checksum="F34704F47FEA44DF" modified="2017-01-18" version="1">MVTKDTAIHRPTGNGTTHKIREQLNFLDNKKWKQFSSRRLELIDKFGLHEKKASEQDINIKRIANVLRTEYNYPNDFQLDFEKLVTAAVQSVRRNRKRSIKVESRDCNNTTSEASVYTTNTNNTTNTNNTTXTNNTTTTNNTTTIXSTTSDASKSSITSSGNSGNSLNTLGSDSHSXSRTSSIGAPTSTLLHPKPVKIEPTLSFNNSNAVSGXCGVGQMNTINSIHNNSNNNNNNNSSNNNNNTNTFINNSLHQPLMKQVPILTSVETVKQIISDMVHLRIPIKEQEKKENMFCTLPTSSTFASSSYLQNSTPSSTTSASSFNSGYPSPMEFFALTSQNRDLLPLALKQQQQQQQNPQISNSLNTLRKPYSIPFFLKEKTLFQINRSKTCLEISSQQGSFIDLYPNILMLGNTIIQAAVSFVIERFYSNIPISSIEYLMNKLIHNNNDVIVKLCVLFFNSATNHNVENLPQIYQLHLFKLIFGSLCKDFGFDPVIYPLSEIFNDYILREFPVIASSASSFTDSSVTAVSNPLTNSQAENSTSSSVIANIKRSXSASTGMILNTVSMKPSVANQDIYRKVVILYKDKSQEFTFHLLSNGPPTILEILNTCKDLFKITHSAHLQLYHNLENSDQSVFIKDDMELASIFNQFTLTKTFIEIKEYVPPVMVAGDSAGMMSNIQHTTAMLPNSHTSSGSTSPVAVLNGLRILSNAATAVPSASSALQNQQMKRTETLSSPPPDIRKCSGLFSSKNLPKPLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB27</accession>
    <name>A0A1E5RB27_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase</fullName>
            <ecNumber evidence="4 14">4.2.1.134</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g3121</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a very-long-chain (3R)-3-hydroxyacyl-CoA = a very-long-chain (2E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:45812"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:83728"/>
            <dbReference type="ChEBI" id="CHEBI:85440"/>
            <dbReference type="EC" id="4.2.1.134"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Endoplasmic reticulum membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the very long-chain fatty acids dehydratase HACD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.134" evidence="4 14"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB27">
        <property type="interactions" value="535"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB27"/>
    <dbReference type="InParanoid" id="A0A1E5RB27"/>
    <dbReference type="OrthoDB" id="46988at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102158">
        <property type="term" value="F:very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030497">
        <property type="term" value="P:fatty acid elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007482">
        <property type="entry name" value="Tyr_Pase-like_PTPLA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11035">
        <property type="entry name" value="VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11035:SF3">
        <property type="entry name" value="VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04387">
        <property type="entry name" value="PTPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 14" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7 14" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ83773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="223" mass="25565" checksum="9C500174B10C8964" modified="2017-01-18" version="1">MSTKPTKGQPKAKQLKPLLHPLVLYNLISALMWGFHLWRTLTWGLEIGPSRFYDISKNPLTFIQCCAVIEIFNSLFGFVRSPLXTVVAQVSSRLLIVLGVFQYMPTAQGPRGFQYITLSVAWCIAEIVRYLYYFYNLVQNDPPVFLSILRYNLFWVLYPLGVGSELYIIYSSLDSAPSTGYKYFLIGSMLTYIPGFPILFTHMMKQRKKVMKQIFGASKAKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBE9</accession>
    <name>A0A1E5RBE9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATPase expression protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2500</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for translation of the mitochondrial OLI1 transcript encoding subunit 9 of mitochondrial ATP synthase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the AEP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBE9">
        <property type="interactions" value="151"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RBE9"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045182">
        <property type="term" value="F:translation regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031467">
        <property type="entry name" value="Aep1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17049">
        <property type="entry name" value="AEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 6" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ84224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="518" mass="59646" checksum="8E20479DA649BEB5" modified="2017-01-18" version="1">MLAKRLIFKQAPRLQLRTLSTESSTVPRLQKIVPLQKDYNNVISPFYVPLDHEILQFVYQNEPKKLLDGGPFLHKLHSDAQVSPDYCKSTSYSLKTDAGPGSTEWLFQWTAFLRKLNSLDLSVAPFLHKQFTXKSPFQNENGQVDLAKKYSIQELSTEIDCIIQADPLLFKHQIILYMVQQNNFFKKNQEAILSAYLKYMKHEVNDPKECNGFFIEVFKTVEALGITLSPESSEILNQIIEQFVSRNSVEAFEKLAIVGVLDFFISRKDVYQANSYLTVLLANGLAPQSNTLSSFIDLVEDPVFFSTLGSILKKXSNAEIFKKLLPYIDHHKKLQFLIKSNEHNLENFNICVLSFHKLLDLQFQYEHELASYKKDYALQKKTPERFKYLLSEASLLRYNRKVTNIYNLFSKSLFEHPKVLLKTIKAYYKGGNYFMINQLLQKLELNAENLLEIFSIKQSGATELELVSGPHKKAFFNKSVLPIYSKCSDQQKAALLAHIQKYGNKTSVELFNAMQXSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBL8</accession>
    <name>A0A1E5RBL8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3113</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBL8"/>
    <dbReference type="InParanoid" id="A0A1E5RBL8"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071008">
        <property type="term" value="C:U2-type post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028211">
        <property type="entry name" value="Ntr2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15458">
        <property type="entry name" value="NTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="306"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="335" mass="38393" checksum="9400229175F4C14C" modified="2017-01-18" version="1">MFKKRGVKKIQITPDDDLDSQGKEIPQRQQLKKPLPSFHEDEDLEDDIDDEIANLSKFQHNSQPEKSSPIFQKKRINTLKFDSMPQASFSANQQADSTLNRKKKTINDLTVNINSTPTPTPTFHSLAEPKPEVWNMDDMEDEEDSDLVVLTEKQKEMLLQKKMTHGTFSSLGSRNSQRDILKQENEYAKLLNQDDLEELNAIYNKGKGATATLDNAKKLQIPGNGRLSEDEDRLDQALLSNEHFVEDHNTMMGGHSKLALSEREKQIESLQRKRYITEQLDAYGDHVVRAPHPKDMTQRGSVLQIRQELQNSQETLVSELDEIKLELSKLTKELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBP7</accession>
    <name>A0A1E5RBP7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Profilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2693</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the profilin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBP7"/>
    <dbReference type="FunCoup" id="A0A1E5RBP7">
        <property type="interactions" value="284"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBP7"/>
    <dbReference type="InParanoid" id="A0A1E5RBP7"/>
    <dbReference type="OrthoDB" id="421374at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003785">
        <property type="term" value="F:actin monomer binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00148">
        <property type="entry name" value="PROF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.30:FF:000001">
        <property type="entry name" value="Profilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048278">
        <property type="entry name" value="PFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005455">
        <property type="entry name" value="PFN_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036140">
        <property type="entry name" value="PFN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027310">
        <property type="entry name" value="Profilin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11604">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11604:SF0">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00235">
        <property type="entry name" value="Profilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00392">
        <property type="entry name" value="PROFILIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01640">
        <property type="entry name" value="PROFILINPLNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00392">
        <property type="entry name" value="PROF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55770">
        <property type="entry name" value="Profilin (actin-binding protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00414">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ84324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="126" mass="13695" checksum="F3E698E28C958432" modified="2017-01-18" version="1">MSWQAYTDNLVATGKLDKAVLYSRAGDAVWASSNGLQLQPAEISEIARGFDNPTGLQSNGLHIAGQKFMLIRADDRSIYGRHDAEGVVCVRTKQTILIAHYPAGIVAGEATKIVEQLADYLISVQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBV8</accession>
    <name>A0A1E5RBV8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">60S acidic ribosomal protein P2-beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2681</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes). The 5 acidic ribosomal P-proteins form the stalk structure of the 60S subunit. They are organized as a pentameric complex in which uL10/P0 interacts with 2 heterodimers, P1A-P2B and P1B-P2A.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBV8"/>
    <dbReference type="FunCoup" id="A0A1E5RBV8">
        <property type="interactions" value="991"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBV8"/>
    <dbReference type="InParanoid" id="A0A1E5RBV8"/>
    <dbReference type="OrthoDB" id="1227494at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002182">
        <property type="term" value="P:cytoplasmic translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05833">
        <property type="entry name" value="Ribosomal_P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044076">
        <property type="entry name" value="Ribosomal_P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184709" evidence="7">
        <location>
            <begin position="17"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="72"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="72"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00063629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ84380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="109" mass="11085" checksum="882C8019B1ACC240" modified="2017-01-18" version="1">MKYLAAYLLLVQGGNASPSAEDIKSVIESVGVEVESAKVDALLSALEGKTIEELIAEGNAKFATVPAAGAAVSSGSGAASSGAAAEEAEEEKEEEKEESDEDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RC38</accession>
    <name>A0A1E5RC38_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative trans-sulfuration enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2708</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RC38">
        <property type="interactions" value="185"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RC38"/>
    <dbReference type="InParanoid" id="A0A1E5RC38"/>
    <dbReference type="OrthoDB" id="3512640at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000072">
        <property type="entry name" value="Putative cystathionine beta-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000066">
        <property type="entry name" value="Putative cystathionine beta-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054542">
        <property type="entry name" value="Cys_met_metab_PP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808:SF35">
        <property type="entry name" value="CYSTATHIONINE GAMMA-SYNTHASE (AFU_ORTHOLOGUE AFUA_7G01590)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="TRANS-SULFURATION ENZYME FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="3">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ84143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="402" mass="44927" checksum="58F73A0D2E4218CD" modified="2017-01-18" version="1">MTEKSVDHPSLATTLIHADDKDNRVSDVAPPINVSTTFRYGENDLIPWNERTDFEFLXTTPIYSRLGHPNSTRVESIFNTFLGGHCVVYSSGLAAYYAALTCYNPKRIFIEQCYHGCHSICNIFKRNYGLQTFSFDEIESKAQPGDIVHLETPVNPYGTSSDIQKLADRAHAKGALLLIDSTFAPPPLQNVWDFGADIVLHSATKYFGGHSDLLSGVLVVKDIEDAKKLKNDRIYLGTNISNLESFLLLRSLRTYEMRIMKQSDNVTKIVQYLEQNKTTKFKGVLEEVTHSSLQKDEFVKKQLKGGYNPVFSIVLSSKEQCKKLPSLVKLFNHATSLGGIESLIEWRALTDASINQKLIRVSVGCENXDDLINDLSQALIAVRNLDXSSXNLXEVTDALGNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDG4</accession>
    <name>A0A1E5RDG4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Spc7 kinetochore protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1935</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDG4">
        <property type="interactions" value="83"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RDG4"/>
    <dbReference type="OrthoDB" id="5592879at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990758">
        <property type="term" value="P:mitotic sister chromatid biorientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033338">
        <property type="entry name" value="Spc105/Spc7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013253">
        <property type="entry name" value="Spc7_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28260">
        <property type="entry name" value="SPINDLE POLE BODY COMPONENT SPC105"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28260:SF1">
        <property type="entry name" value="SPINDLE POLE BODY COMPONENT SPC105"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08317">
        <property type="entry name" value="Spc7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spc7 kinetochore protein" evidence="2">
        <location>
            <begin position="409"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="3">
        <location>
            <position position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="829" mass="95230" checksum="64A05D926676DBF1" modified="2017-01-18" version="1">MPSXINGGFSERNEASNSQQXTKSILKTSQPETGIAATFGNNVRAKRQSLIDNGENNTTSRIDTVSLHKKLLTQEKKRRVSFAPDVTLRTVDFQDVIAEKPXGAQHDNKXFASEDPDESEMELTNPINNVLAVQRVPGNQLNENADDEADSQKLDMEITEIFTKNTATQEQNNSTANLQDVVLLEKGMKTTSLQNENHRNQNXSQNSEDELHTNTSMDLTTFQNLDKKVQDTNGMEERKDISDMDIDLSEGADMELTYMDIGSKNASNEEDHLVFDQQKENKRIPADGFEKVLTGNKPHSSTTNSRPSSLESGYASEEKLSPIPLKRLPSNPEQEHMPLPKKKQKHNKLSLSESISTLPSVDAYVHIPDDQNLLVKFLKSSHLDYLNNIPXLRDFNHSHDVKPFDFNVFKYKSDVSTAEVYKELYTNLPFLIILKYMWFELNRKISKSKSRFALLEKELSDNPSKLPALLSLFLNSSISSDGKTNLRNYIFVLKQNSDLVAESVWLNWRVNQVEGINSDLEDGLXNLQAXIVNNESDSESIVKMREEAETVYNDYVREFEKRKIMHNQHTLKSVETENLNKVNXNISFEHKLKWVEKKNYLFKKYNVDIGDFESYPSRKFDILKALNSACSRQPSKADIEDTQLKTFEAKFADQRNNLKFIRSSNMIIAIIRVPSIKVFVADDSNDTSSIKMNLDNQDEFTKMWFKKLLHNVEDSNLKDKYEYLRYVMEARRKYEIISFNYKKLRLLFVTKTXETKSGGIIQFQSVNKWNKTLFQLTFSQFTQLVLYNKPVTSKRTVINYSTSVSFXENSSIXFSTEITEXLPWLINIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDJ7</accession>
    <name>A0A1E5RDJ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycine cleavage system P protein</fullName>
            <ecNumber evidence="7">1.4.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2016</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The glycine cleavage system catalyzes the degradation of glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 7">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[glycine-cleavage complex H protein] + glycine + H(+) = N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[glycine-cleavage complex H protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:24304"/>
            <dbReference type="Rhea" id="RHEA-COMP:10494"/>
            <dbReference type="Rhea" id="RHEA-COMP:10495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="1.4.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the GcvP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.4.2" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDJ7">
        <property type="interactions" value="698"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDJ7"/>
    <dbReference type="InParanoid" id="A0A1E5RDJ7"/>
    <dbReference type="OrthoDB" id="6537869at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016594">
        <property type="term" value="F:glycine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004375">
        <property type="term" value="F:glycine dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00613">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000007">
        <property type="entry name" value="glycine dehydrogenase (Decarboxylating), mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003437">
        <property type="entry name" value="GcvP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049316">
        <property type="entry name" value="GDC-P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049315">
        <property type="entry name" value="GDC-P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020581">
        <property type="entry name" value="GDC_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00461">
        <property type="entry name" value="gcvP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773:SF1">
        <property type="entry name" value="GLYCINE DEHYDROGENASE (DECARBOXYLATING), MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773">
        <property type="entry name" value="GLYCINE DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21478">
        <property type="entry name" value="GcvP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02347">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 6 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Glycine cleavage system P-protein N-terminal" evidence="9">
        <location>
            <begin position="47"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine dehydrogenase C-terminal" evidence="10">
        <location>
            <begin position="837"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="669"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="752"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR603437-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ84961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1038" mass="114622" checksum="703326CAD3005E6D" modified="2017-01-18" version="1">MLRRSTKVFPLKHIRLNSTLASVDSVNYAKVHSPSSNTLKNLDTFQRRHLGPNVSNVSEMLKTMKYDDLQKFVESVVPPQILTRRXLKLHDVPAQGFTESEMLQSLKKIASQNKYTVKNLIGKGYYGTHLPPVIQRNLLESPEWYTSYTPYQPEISQGRLESLLNFQTVISDMTGLPVANASLLDEGTAAGEALLLSFYQQKKKKSTYIIDSNLHEQTKSVLKTRAEPFGIKLTEVDFIGKSADEYSSILKDNDVFGCIVQYPGTNGDILPQDKLSKIGELVHANKGLFSVASDLMALALLPPPSTFGADICLGTSQRFGFPTGFGGPHAAFFSVVEKLNRKIPGRIVGVSKDRLGGKAYRLALQTREQHIKRDKATSNICTAQALSANVAAMYCVYHGPKGIKNIAQRIYGMTTLLAQQIQNFSGHKLLNKNWFDTLTIELQAGIDSDSLLQLALSKYNTNLFKINNKTVSLSLDETVEEKDXINMIELFTGLKKTSVDVAQTGLPAFPNARVEGFLQTQVFNTHHTETAMLRYMHLLQSKDLSLANSMIPLGSCTMKLNATVEMMPLTWPEFTNIHPFQPIDQIEGYRQLITSLEKDLANITGFHSISLQPTSGASGEYTGLRVIMAYLKAQGQSNRNICLIPVSAHGTNPASAAMCGMKVVPVKCLENGSLDLQDLEAKAQQHEKNLASIMITYPSTYGLFEPGVKDAVDIVHKYGGQVYLDGANMNAQVGLTSPGDLGADVCHLNLHKTFAIAHGGGGAGSGPIGVAKHLTNFLPRHDVEPIFDDLVDSEEVVQAKRENAIQAVSSVAYGNAPLLTISYAYIKMMGSLGLPYASVIAMLNANYMMNRLQAHYPIMFIGSSNEKISNGSDDSKNIANTIKHCAHEFIIDLRDFKKYGLEAIDVAKRLQDYGFHGPTLAFPVPGTLMVEPTESENKEELDRFVEAMISIKQEIQYLIDGDSRGNVLKNAPHSLQDLILSKDWETTRGYTRELAAYPLPYLKTNKCWPQVTRLDDTYGDLNLLCTCPSVEEVVQHQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDK2</accession>
    <name>A0A1E5RDK2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1889</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDK2"/>
    <dbReference type="InParanoid" id="A0A1E5RDK2"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184710" evidence="1">
        <location>
            <begin position="21"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="103" mass="11841" checksum="8B277DFE306352CA" modified="2017-01-18" version="1">MHAVFFTIFVTSLLLNVCFAQSGFIFGKDNYSHVAEIPKSNNTNSLHLAFGKPKIIKFTEYSLFVALEYVYKKYPELQSAQQMLPENYAHDDQYVVFSPMGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDM5</accession>
    <name>A0A1E5RDM5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosome biogenesis regulatory protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2083</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in ribosomal large subunit assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the RRS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDM5">
        <property type="interactions" value="623"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDM5"/>
    <dbReference type="InParanoid" id="A0A1E5RDM5"/>
    <dbReference type="OrthoDB" id="28455at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007023">
        <property type="entry name" value="Ribosom_reg"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04939">
        <property type="entry name" value="RRS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="126"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="165"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ84994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="206" mass="23234" checksum="D61DC36B264B705F" modified="2017-01-18" version="1">MTDTATTKSKYAELPVTVEKPIPVTYDLGNLAVFDXNTIDGDAFDSSNKTREEHIKXATRDNVQLLINQLLAQPIRTTTDSAKSSISLISLPEPTTELPREKPLPKPKPPTKWELFAAKKGIKSKSRDGKQVYNEETGEWSAKWGYKGPKEKGKLEADWCVEVDDTKPGNEDGLIDPRTLDRQERKRLIKKNELQHKRNLKNAGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RED1</accession>
    <name>A0A1E5RED1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Polyamine transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2300</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RED1">
        <property type="interactions" value="101"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RED1"/>
    <dbReference type="InParanoid" id="A0A1E5RED1"/>
    <dbReference type="OrthoDB" id="9986881at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF31">
        <property type="entry name" value="POLYAMINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="272"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="307"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="413"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="452"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="492"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="521"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="550"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="587"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="183"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="623" mass="69451" checksum="6B22513BF29FC783" modified="2017-01-18" version="1">MSTSYSNTSGSSNLDEKDEQMIAAQQNAEQVFEDDQVIDRKNKKLXNQSGSSEAEDDEDEVASFTGSNEDLEANKIATEKYYETNNNIENAPEQENLDDDKLIDTASLAESESHTDMQRKLSRILTEVDSTMKNISYEDCPPMGGGRPYPPPLPKRDPWEVTYDGPDDPLHPFNWPMKKKLWLVFILAWNCMAVTMGSSAFASAVTQICEDYDVITVVAVLGVTLYVLGFAISPIAYAPLSELYGRQIVLIISSFGFVLFQFAVATGENLQTILICRFFGGAIGAAPLAVIPAAMSDMFNNKQRGTVVSLFALTVFMGPILTPVFTSYITEYTTWRWIEYVIGIFAGMSFVLVAIFYEETHHPIILCRKAKMMREKSGNWGIYAAHENNSLSIKDIVTKTISRPLRMLRSEPILALLTIYNSFVYGILYLLLEAYPIVFLEGYHFYKNAELPYIGLIIGMGVFALTMVFYFEPKYVEAVVANGGKPCPEERLKPMMISAFIFPIGILWFCWTGNYPHKIHFMVPTVGGSFIGFGLIGIFLTSFNYIIDAYLFLAASAIAGNTFLRSAFGAAFPLFAGYMFKGMGTGYAGLLLGLFAAALIPVPFLFYKYGVQLRTKSKFAYVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REE6</accession>
    <name>A0A1E5REE6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Target of rapamycin complex 1 subunit TCO89</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1931</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REE6">
        <property type="interactions" value="151"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REE6"/>
    <dbReference type="InParanoid" id="A0A1E5REE6"/>
    <dbReference type="OrthoDB" id="5430106at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018857">
        <property type="entry name" value="TORC1_cplx_su_TCO89"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22794">
        <property type="entry name" value="THAP DOMAIN PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22794:SF2">
        <property type="entry name" value="THAP DOMAIN-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10452">
        <property type="entry name" value="TCO89"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="555"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="602"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="689"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="903"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="602"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="689"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="907"/>
            <end position="921"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="935" mass="103500" checksum="151DC5653A5578A6" modified="2017-01-18" version="1">MKVAESVPTMSSVSHYGNKKKKQFSASNRNRSATSFKGINKLCRVTSFDQQIMGNDFAHENSSLHSNGSNLYLDRVRSIDSLNKRRTISSLSMTALCKLKSGNSINHNKHMSTSASNVTNRLDNSVDDHKDLLPEIKPKGQKTRKQKAPIRNTDQDTFVRNSSTDEDVEHFTNDDVSSSNDSLQNDNTVGEESQNLTLANGQPYEFEEDISNELDTKVEQLDYEVLPDEHPKEIEENKNELGAIVASSKGDHKVSKDDAQRETDEEKYAIEPNENLRHAENQEIPFENQISEPSQYAETGHYTSIPHSDSLIQQCQKNGTLDAKNASNTLQPVLANTNNRPLSYVVPSSLRRNSRISADNIGTLRTSQESQNSVGLLLNSNYLNNEHAVGQHSSNEDRIKPSHMDDASEDDTIPEYIGAIEEEEIGGLKTNENLLSADTAQLTKKNQSEKPNSFNDRISQGMELENVQRSASKIEPEYLSDHSKSSTQKEGQAISEERAQNNFFQNTDIMQSSGQDDTLSSNAVPNTYASVGKSVTSNHQFNYINKDFLNSSDSLSTLNRPATGDTQSINSNEKPFTSNAQPIQNSIAQNAQITSISESAESLNQNSLEESQATSLVNNLXRPSSQNSQKSDFSSSISSLAIHLQRPVTRNNASLVNRSSQAQIPSKNSQNSLLRLATVNNIGLNSKAVPQQSKGSYARASNPNPNPNPKRNSLNASSLKAGKPALNDFSSFLKSDSTDAESRTQQKLWLQRENSILDLSSDALNNQLDAVFMATSVEAKRESERISREYAAVKRYNNPLYTSIQRINKNLRIEEAQPSKSKPSTTDLTKLFSTPIEKAASTDSNLMENLISPNVENMLKGIWHKETQKFMQDDVSGSTPQASSNSLYQNRSSALMRNNSGSQLYQGHHRSGSIYHSHSRSRGMGSYNSGVGVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REH1</accession>
    <name>A0A1E5REH1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Mannose 6-phosphate receptor-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2434</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REH1">
        <property type="interactions" value="243"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REH1"/>
    <dbReference type="InParanoid" id="A0A1E5REH1"/>
    <dbReference type="OrthoDB" id="4504960at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.130.10:FF:000024">
        <property type="entry name" value="Putative vacuolar sorting receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15071:SF0">
        <property type="entry name" value="MANNOSE 6-PHOSPHATE RECEPTOR-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15071">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MRH" evidence="9">
        <location>
            <begin position="70"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="86"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="362"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="454"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="86"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="385"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="416"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
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            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="482"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="493" mass="55165" checksum="CF748DDE3E1AEB29" modified="2017-01-18" version="1">MHGALRKNIVIYLLVGLLCLIGLAGRVTWFASLKHEITKTNVDNAQRLLLKRSTTAHLSNNDNDGSGDKMFCAVKHPVYGTYIDLSQLSTTPNTPSGSGNKNKNKNKKQQNQDQPTLPVKTRWSVNGWDYTTNFTLGICSSAVDDIESGSSTTTLSSVTSEKNXTGAFYYESNEARSRNRYTSIGEYSTTPRFMGKKLVLQYDNGDFCPNGIDKKTTLLNFVCDKEISSRAQINFIGSLHNCSYFFEVRSIYACPTTVVKDDYNVIGIFLFIIVVFGVVECGRRRLTKFVHSNRVSNGINGSNGDNRVYMHNENNSNANSMDSLNYLNYINPRWARIENESKFKSFAKTIFNKLHNLFSHEGNNTATTTNNNNNNNNNNNNNNNKSFYYNTRTSRNNYKLQHTGNGGSHTIPTQINSNNSNNSNNSRNSRGSNDTLFRDIEIQNNLMDSLQMDGIDEDEDVGGVTTRGLDTLSTGNGIDNNSEQRDLVTDNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REI0</accession>
    <name>A0A1E5REI0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">WD repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2015</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REI0"/>
    <dbReference type="FunCoup" id="A0A1E5REI0">
        <property type="interactions" value="886"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REI0"/>
    <dbReference type="InParanoid" id="A0A1E5REI0"/>
    <dbReference type="OrthoDB" id="1284551at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045159">
        <property type="entry name" value="DCAF7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19919">
        <property type="entry name" value="WD REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="393"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="501"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="75"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="243"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="547"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="8"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="247"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="547"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="562"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="585"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ84963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="665" mass="73009" checksum="CDA4405A6E490049" modified="2017-01-18" version="1">MQSPSSNEQQQQQQQQQYLQEQQQQQLPVNFDPRKRSSISFGGYRPTNNIKTYDSLKSMDRNGMYYAANNMATGNMSSDNNSSNNNSSNDNSNGSNLSPGNSYINLNGNFSENPHGIANLYNSLSLMPPFSQQNQNSLGNWSFQTGNANGNGNANGNANGNNMPLCSYRSKFPLYGLDWSSNNAYGDLVSLGSYRENNVNDIQILQNTNSTGGFPEWEKICEQKTLFPVCKIQWDPSLASGTVITPTGESNGNYNSTNNNSGEGTSRSTPPLENSNNTKANLATCSDSLRIWTLQNKLDATDNNNNIHRSKNNEIQDKYELNETLNLSLWKYYNKQGLSGVLPVGGDTKVLGSLPPVTSCDWNKKDPSIIISSSIDTTCTVWDLNRINVRTQLIAHDSEVFDVKFLTNSSHLFATCGNDGSARVFDLRSLEHSSIIYESPDALNSPTSGESISSTANHRNNALLRLETCPFDINVVATFASNSNSVMILDMRYPGQPVTVLQKHSGNVNSIKWHPSKRGILTSCSDDCQVLVWDTTSTVSCVSPINSSSSICSPNSNHNNSETPNSVNSENSFNRTTVSPPVDANSTTATTTSSTSNINNSTNNQPVDQNRDSFAQGKKIVYPAPILCYNDNVHEEINNIVWRGGQSNGDWLGFVRGKNFQSIKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REK9</accession>
    <name>A0A1E5REK9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">60S ribosomal protein L7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1908</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REK9"/>
    <dbReference type="FunCoup" id="A0A1E5REK9">
        <property type="interactions" value="1446"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REK9"/>
    <dbReference type="InParanoid" id="A0A1E5REK9"/>
    <dbReference type="OrthoDB" id="28644at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.20:FF:000003">
        <property type="entry name" value="60S ribosomal protein L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_uL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018038">
        <property type="entry name" value="Ribosomal_uL30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005998">
        <property type="entry name" value="Ribosomal_uL30_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_uL30_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012988">
        <property type="entry name" value="Ribosomal_uL30_N_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01310">
        <property type="entry name" value="uL30_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08079">
        <property type="entry name" value="Ribosomal_L30_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00634">
        <property type="entry name" value="RIBOSOMAL_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL30 N-terminal eukaryotes" evidence="6">
        <location>
            <begin position="61"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="5">
        <location>
            <begin position="109"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="28"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="269" mass="30583" checksum="7AEF5E8F6A68A3E6" modified="2017-01-18" version="1">MFYLLKLLCGTRKSLFLSRKILTPESQLKKSKAQQKTVEERAAERAARKANNENNNIFPQSNKEKRATILSRNTAYQKEYEAAERAIVEAKREARKTGAYYVEAQPKLVFVVRIKGINKIAPKPRKVLQLLRLTQINSGVFIKVTKATSELLKLVEPYVAYGYPSYSTVRQLVYKRGFGKINKQRIALSENSIVEASLGKFGILSIDDLIHEIVSVGPNFKQANNFLWPFKLSNPNGGWGVPRKFKHFIQGGSFGNREEFINKLVKAMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REU1</accession>
    <name>A0A1E5REU1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SAGA complex subunit Spt7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2125</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REU1">
        <property type="interactions" value="531"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REU1"/>
    <dbReference type="InParanoid" id="A0A1E5REU1"/>
    <dbReference type="OrthoDB" id="21449at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05510">
        <property type="entry name" value="Bromo_SPT7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000032">
        <property type="entry name" value="Transcriptional activator spt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037782">
        <property type="entry name" value="Spt7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47343">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR SPT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47343:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR SPT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 8" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="10">
        <location>
            <begin position="549"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="102"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="228"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="411"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="660"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="758"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="895"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1380"/>
            <end position="1442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1511"/>
            <end position="1572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="239"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="277"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="299"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="421"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="665"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="708"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="782"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="895"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="906"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="938"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="957"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="976"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1380"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1396"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1511"/>
            <end position="1533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1572" mass="177619" checksum="7AF7FAD49D13AC5C" modified="2017-01-18" version="1">MSMLSVGKHKAYMRNYQQTDCQKLYHVTKYLLDHDLLSQSLTPEQCIVIESLVAVVENERTDESNENSTDGTIHILDKGWSIWEAFMKGKLKLNVVKVDHKKKAKTSKSQKQNTSTAEPMETSTIKKQNEENNEQQQGYETDFSEIDLLNLQENLNGYEFIANLSLKLRYVIWEKPLSLNHTSIGHTNIESLHDTQQMSEATTLLADRLIKENASYLDDEKISKVPPYIIGSRSKDNGIHNNTEIGLGNNSTNASGLNDDESGNNVVTKNQDKKDDDYDADDADDYDDDYDADDNKPQDEEEEEEEEEEEEEEEXVVVDGKGEVFEDGNVNNKLTTGNHLAGNLSDTISDRSLDEEGFSTLNIIVSQKTLKKLRISNVDSIMTRFETIYHSLENDKETVMKRMKLQENDQLLESNKKRKFNPSNDTAFGNEQNVHNDQLSTTNSTTSKVKIEDEGNGVTLNGTNNKEKTPGVQQNQTAASQFSVFSNLPIQHLLGAIEEGRDQVSLNDYELKHLLLDVRKSRSKWASDDKIGQEELYEACEKVVLDLRNYTEHSTAFLNKVSKREAPNYYNIIKKPMDLNTVLKKLKTFQYKSKQEFVDDLNLIWKNCFTFNSDPKHFLRKHALAMQKKASTLISHIPDITIRDRSEIEKELEDPLAVVLAAPHSSKSTPSTTTTTTTTTTTTTTTTASSTNTVENGANDSGFAKSLQEPSSQAKASNTMNIGTSELENKSNDAVLGENNKLPENSVQTLDTHFAHQENNANPEDHGLASENDTTGQKLHEEAEEEDEESDDEGYSQYLKEQDIYKDDLELTLWKSLTSNARAELCIERSKLFDGCKLNAKAVALLRNKKKMKLPSALINEFLVRKDTELIAKKQKKNAMLKNGFNSALMYDGNEEEENEDDDETKDQLVDKNDKTSMPNEDGKRDASETQISNVEAAEGKQFETGKSADEPNDLGVEVKEIESVRIEEKSKDVNDDKDDDSKEDDDEEEEEEEEEEEEEEEEEEEQINIEVDDVAFLDEYNIANGLPGIKYSGISNTDISNLENLLVDQELKKMQAHNAPGNGMKSCFFAQSDSGMALKLNSNIQLTQEIRHICHKIYLIRELQNRQAMVPTKNNPTPWKFTPTLFKKFEVDAKVDIDSVSQLPTRNFKYDGQVAKSVLLKTSSKIAMNSGFESTERSAVCMLNDIAEEYLCNLIKTLKVHSESISTNKQDPETSLQVSLLQNGIIRPDELYSYYEKEFLKKTKKLKDIKFKLNNFLKQLLRPSLKGLNERNFDDESTAFVTGEYTSELTGEDFFGFKELGLDKEFTMLXKSIPLHLLAFQFAGKPGGTSVKDNKIQKDELLNIKYHRLTQADLDNKKLFPQLMKEFFTKQLEVSKEVEAEQKSKQEPGKELTTDSEQNENTDLATKVSTEVEGVDKAQEGREDEASELKTAGSIETNNDKNISNDIAHAEHTPSPDFFILEDEELPNKPKPPRLPPTGKVIGMKKKLVSESYFLPESLKAKLETERKLAEKKKQEALAKAKEQKEEEEKAKQVSATETSPVDAYSQLVLPPSVHQNTTNGLTLPPKVSNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REV2</accession>
    <name>A0A1E5REV2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inheritance of peroxisomes protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1736</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for peroxisome inheritance.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the INP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5REV2"/>
    <dbReference type="OrthoDB" id="3972277at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005780">
        <property type="term" value="C:extrinsic component of intraperoxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045033">
        <property type="term" value="P:peroxisome inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024758">
        <property type="entry name" value="Inp1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12634">
        <property type="entry name" value="Inp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02103">
        <property type="entry name" value="INPROXISOME1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="39"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="94"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="254"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="94"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="107"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="259"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="7">
        <location>
            <position position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="420" mass="47925" checksum="D4DD9A834E2510D4" modified="2017-01-18" version="1">MXTITETLSSEPFQQPKRLNRFKNKFKNTIESMKSDLNLPKTRFKTSQHQKNHASSLQNHNQNAKKRPMSAQRVSLFKHPNVIVNIIYEGQTRTSSADTLRKTSTGSLXESSSAPPASSLHDDDAATLTNNDSHKNXVSVPHSKQSLGFHEPFEIYEIKTMDENNISIGCYLSIGKNDKVVKPILPKLKVIDLGDGLFHIPFSNPSTKLWELIMPSTEKEKIDQLRECFVDICQLERPTLKSDSFTESTVLDMDKSDVETEEDDLDDFLNTTEEESDKGEEEASELLAFEKDELEDFXLVPEKQSIVTPQQQEVNDLFKKTVSSFRFSYHSSGLPMLSQSFLESQVVSPVTXFQKKSSLPTKQKRYSSYELVKEFQNQSHTLSKDVVSQRAFPSGNLKHVYRRSISVPMEFQLRNRKSFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFK6</accession>
    <name>A0A1E5RFK6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zinc finger transcription factor YRM1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2218</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFK6"/>
    <dbReference type="InParanoid" id="A0A1E5RFK6"/>
    <dbReference type="OrthoDB" id="3971603at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052693">
        <property type="entry name" value="Yeast_MDR_Regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31405">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31405:SF8">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="36"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="960"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1069" mass="121125" checksum="347514B22886E48C" modified="2017-01-18" version="1">MVAERDGLPPFDESVDQSSPTTTGTVKRRRSRAIVSCLYCRERRLKCNQARPKCNSCESRNLDACLYFEDTTPERIAVERAKIRSRKMKVTKNRKNLSTVPDNVMENSSFMNRILPPPSSSDSVQGHQSFANVPKDYSRDGNSLMVQAPTRNFDMQSSNIEPIIPKINSNNSSLHQRQSEYYRHKNQLLGNSKLTFNKLQSQHNPLQIRMAEKEIYSHSVNLFSNKPKPLSPGLSPPTSQSVPIVTDSLATTVHPNHSDNLAQDFSTTEAHLQAQNLASSGNTLSNSTFQTHFEPQIDQPTSFGNLGPTSNHNLPTETIAPDLPHENLDQMSSLKVNDHPDASRGFGVPHSANTSQHCTLKDTTLSCASGQDLGNTCGLEKSAGTSPSLAAPEREEKCSYVHPFTLGFAQSVEPVNPLRQYTIVLVTEKAACMVGATSVICLFKNSKCMEENKSIWDAFRVTCEQSSKGDPNGLDGVFKERHVLLRXRGSSLLQEIVATLPSYSXFKKTLFSYFQTAYHSLTNFIEEKAVVQLFEDLVEVDLETDRITSLNVDDRTNFFSVGVIXLLYKIVKGHTVDHDCFTEFFCYIRGITSGLTFSLHRIQFLLLNYFQTQFDPLRGAHTRSVHLIAESLGSMTDMLGLRTGAQKYYKDKLEHVGLIHTLQNIWYWSLYADVLNSFENGKSLNVSYGSFDEEELNTLEAGRIGKLKRFLYLARKIIFDLNKPRGIPDIEGMLIQVDKYLNVELHSLETYCDAEHLEKVDLFDYVVLTPLIALKICLWYLQLKVFKADEVVHKNKLFRILVTSFKMITALSTHCSYLKSSSSGISSEWTVGESATNISFWLKKPLTFRALITLFDLMYERGKADRKVTRRNLKKRKNLIMENLVDFERPEAEVDYTNNQYSSSDMFIKYTELMNEYYSALKDHLEVKLEKPYGSYSFRAVELHIRSIFISAMENSANTNQGRLAEGKSDQAKNKNHSSSNNHYHDQNDNKKALNSRNEINDSQISQTDTNAERANNIYSYLDPPAAEQARQNNMWDYNPTANGVNETIPDQDLFSLDFDFSKFLSPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFP4</accession>
    <name>A0A1E5RFP4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Autophagy-related protein 33</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2069</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ATG33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFP4">
        <property type="interactions" value="128"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RFP4"/>
    <dbReference type="OrthoDB" id="3972626at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051668">
        <property type="entry name" value="ATG33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37278">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 33-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37278:SF1">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 33-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="123"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ85715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="208" mass="22198" checksum="725C2CD31605B77D" modified="2017-01-18" version="1">MSVCLGIIKTVGVTSLGIYTGMVTTSTILLYNRPCKEIFGSKEESLSSHPSIAQKIVSKLSKIALGLLTISGICFGAAYYDVPXHHKHPYILYAALVTPLTYLYGMGLTFFCPVKGCVPPPKHHPSDLQNNTSATTSTNEPKCPVSGQSGQSSTGEASCPFSKLASQKSTNYCPLHFNISTHLIVLSLLTTVGFAQSVFGLYGEGQFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGM4</accession>
    <name>A0A1E5RGM4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Topoisomerase I damage affected protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1082</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGM4">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RGM4"/>
    <dbReference type="OrthoDB" id="3973565at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="64"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="269"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="604"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="335"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="79"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="123"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="273"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="614"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="624"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="643"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="668" mass="74165" checksum="9B13AAE327DCEC8B" modified="2017-01-18" version="1">MSTNPNAAAQDALTHAGVRPSRPKRSGSMRKSLLIMNTPPSMKKYEAMVNNTNNAEISPQAVPAQYSSQNPHPLVGNAQSSPIPRQQSPQHPYHNFQHHRRKTSNLDKLRSYDYSTEGESDAEPMSPISPEISSQQSGKLNMINVMKNQHHQRTLDQLSGTEPQAPPVVENAVDLAIPDDHTAHRIITPTPVHDVSSSGNANLFDLQKTTITTENSTPQSTVKKPLVRRSGSMTKRHVYKTQHGSESNIEIXGNMSQHRRIRSMSIPIPIVDPTSSTTDQNDTLGRVDDPVQPKRSRPTSMRGYSHNGSTASLVEPSRTPSPVRPPTSDQIFALLASKESQILETKNQIDEVKRQLRSKEADLIEQYKELEELKQNMSMELIKSHNGEIDEVNKSNLKLKPVDMTANSNKGSNKLSFTGNSTPVPLKRAENAFSPNDQGSSGILNIKKKSSQFLSSFSNPTSVISNTNDHSNQVDDISFSDDVKDNGFAEKMAFEQKVGKVVVPSSASGYYKSKATQSTAVDDQNLEQFKNSKKNEVVANTIYEEENSDDNIAPKNETPIRRSNTINRNKRASRMSMYFSKGMNLINQFDQILQSELEKKLGFEDANDGKGSSNNEENLANNFLEEEQDVEPMDNEEANSSVHKTDLQAEEAEKPAADSQSGYKYFFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGQ1</accession>
    <name>A0A1E5RGQ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Complex III subunit 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1141</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The complex plays an important role in the uptake of multiple carbon sources present in different host niches.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein, 2 core protein subunits, and additional low-molecular weight protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the UQCR10/QCR9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGQ1"/>
    <dbReference type="FunCoup" id="A0A1E5RGQ1">
        <property type="interactions" value="318"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGQ1"/>
    <dbReference type="InParanoid" id="A0A1E5RGQ1"/>
    <dbReference type="OrthoDB" id="44067at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.260:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.260">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008027">
        <property type="entry name" value="QCR9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036656">
        <property type="entry name" value="QCR9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12980:SF0">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12980">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX, SUBUNIT X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05365">
        <property type="entry name" value="UCR_UQCRX_QCR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81514">
        <property type="entry name" value="Subunit X (non-heme 7 kDa protein) of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ86089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="72" mass="8141" checksum="E102A01D1D36E824" modified="2017-01-18" version="1">MDERAPQSLNATIFNTFFKRNAVFVGTVFFGAFVFQGAFDSAVTSWYENHNKGKLWKDVKAQLNGGDEDDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGX9</accession>
    <name>A0A1E5RGX9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">AP-3 complex subunit sigma</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1139</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGX9"/>
    <dbReference type="FunCoup" id="A0A1E5RGX9">
        <property type="interactions" value="415"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGX9"/>
    <dbReference type="InParanoid" id="A0A1E5RGX9"/>
    <dbReference type="OrthoDB" id="10261046at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030117">
        <property type="term" value="C:membrane coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000804">
        <property type="entry name" value="Clathrin_sm-chain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00989">
        <property type="entry name" value="CLAT_ADAPTOR_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="6">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="225" mass="25078" checksum="7BD9311AD5C7DD1E" modified="2017-01-18" version="1">MIHSVLIFNKNGQPRLVKFYTPVDVVKQQLLLKQVYDLISRRIVDEQSSFLVTPPDLLGAGSSGGSKNEYQASFEDDDYEDEEQDTIQIIYKNFATLYFVFIVDEQESELSILDLIQTFVECLDMCFRDVCELDLVFGWQTLQSVLEEMVQGGIVIETDKYEIVKAIDLLNSSTTSKSVGGLASFFDPSQYGNSSSTGTMLSGSNLSNAFSMFASGGFSNWAGHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH17</accession>
    <name>A0A1E5RH17_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ribosome biogenesis protein TSR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1277</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class translation factor GTPase superfamily. Bms1-like GTPase family. TSR1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH17">
        <property type="interactions" value="1079"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH17"/>
    <dbReference type="InParanoid" id="A0A1E5RH17"/>
    <dbReference type="OrthoDB" id="119302at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012948">
        <property type="entry name" value="AARP2CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039761">
        <property type="entry name" value="Bms1/Tsr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007034">
        <property type="entry name" value="BMS1_TSR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030387">
        <property type="entry name" value="G_Bms1/Tsr1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858:SF1">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN TSR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08142">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04950">
        <property type="entry name" value="RIBIOP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22298">
        <property type="entry name" value="Tsr1_G-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00785">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01362">
        <property type="entry name" value="DUF663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51714">
        <property type="entry name" value="G_BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Bms1-type G" evidence="6">
        <location>
            <begin position="85"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="411"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="7">
        <location>
            <position position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="794" mass="90652" checksum="AC30F4061C4B007B" modified="2017-01-18" version="1">MAGSHSHRSSMKKNHKSFKSRXSSKSALKKMFKGRVETDDSRKTGKPTVKVVSKLQRKNFAKQVRDQKIKASSENRKLFDGANGAAKIVTVIPLTTDVYADDIVKKLLAGEFPANDLLQSNIVVPSTNNIFISKFKSNLKIIIPDMNDMINLLDSCKIADFVVFGLSGTSEVDPEIGEQVIRALELQGISSYIGVVSNLSQVHPKEKFQLDVKQSLESYFKHFFPNEEKLYNLEKSSESLNCLRTLCQKLPRNVNWRDSRGYLVADSVSYEQDDTTVENDGTNGCLVVEGTARGIGFNVNRLVHLPGLGDFQITKMMKLVKNGDDSCMVPSADQQDSLEEYANHDMEMEADSDMEDFDDEGHDYQYDNLTSARYDEHGFLPGKAEPLRKPKVPKGTSDYQSKWYLDDMVEEGEEVEDEDEDEDEDEELIGENDDQLELGDELPDFQENRDSLYQPTEAGETEMFVELSPEEEERQLLEYRNMEKEDREFPDELELSPNESGIERLKRYRGLKNLHNCVWEVDEKDPTCPSEWNRVLRISNYKNVKRQVLNNAQKQAQVKAGDKIKMYIKFPQEMLDKVVDPKQLVFTVFGLLPHEHQQTVANFSIQRWEDYENPVPAEEPIIVQYGIRRQVIQPCFSSASVSPNNVHKFERFLHEDSVSVATCIAXADFTQSPAIFFRKNSEKPTGIEFIGHGSFLSSDHTRILAKRVVLTGHPFKFHKNVVTVRYMFFNADDIEWFKSIPLFTKSGRSGFIKESLGTHGYFKATFDTKLSAQDVVAMALFKRIWPKTSVGWDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH22</accession>
    <name>A0A1E5RH22_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein STB5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1054</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH22">
        <property type="interactions" value="953"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH22"/>
    <dbReference type="InParanoid" id="A0A1E5RH22"/>
    <dbReference type="OrthoDB" id="189997at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052202">
        <property type="entry name" value="Yeast_MetPath_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782:SF7">
        <property type="entry name" value="PROTEIN STB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="28"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="659"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="665"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="863" mass="97137" checksum="ADED504A554EAAC4" modified="2017-01-18" version="1">MSRDSDEQLFPQQNGTALKTDENKDPYSCLRCRKYKKKCSRDKPQCTTCLKQNKGCFYPGRARRRKRHEIYAAETSAAGTESNPKPKNELELEXIKVRTPLTSGTEEIREKRAKLSLPHSVDVASSAAPLPDVLHTXXIAPKQPNANHSSSALLHSQPKEHQKQDQTAGFHNTSVPVALPGSFTPSNILSPGSFSTPGMIEYLNNFSNSKNMTSPASNGLISNITSPKLGVSPAIPVTGGSTRIAGFYGTSDNNYSGYNNLSRIPNSQNAQGTLNNDILPPLPTKSGPFPRNEPHFATDEAKSSSVXLTSNQLTTDYSSVQVKREYSLAHCPVDKVPYSHAQGVLEHDLHKPESTPIKPSSIQIEGLTAVFKGGRNTNWPLKKGFDSKFKKIDHNLYLDFIDAYFQHNHRSYPMINKFEFLEKVSKIKDFSALDLSTSSEYDAVFLFMLYLIMAIGCTSLSRAKVLKPSENDLSEHFAYLGMKRFCDIMHLQNWDTIRCLILLGIYSFFEPKGISSWTIGGIITRITIGLGLNREVTLSKKTAMEPHTLEFRHRIFWSAYCFERLVHTSLGRISFLDDDDINVPLPVPICEEEVQDIQVTKMIISIRKMGGKIYNKVHSVKSANINEGKTEKEKQLIIDSLGKELDTLFDKHKVQMREMRVKGKGSSSVSLTESRSNSSQSPFSGDSVRPDISFHNSELWLIMRYEQLQIMLYRPSKLFPTLPTGTLTILGXVCLTSLKHTHMLFVKNMLPYNWITLFRTLTICNTMLFCLCQWCVDTVDCQIEIQQCTEILKHFGGKWVFAKNCAKVFENISSSISEFSVGASTNEKMKQLNQELFGASNEYQDILDENNVDVFWLDDXFVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH40</accession>
    <name>A0A1E5RH40_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1407</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CLPTM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH40"/>
    <dbReference type="InParanoid" id="A0A1E5RH40"/>
    <dbReference type="OrthoDB" id="3971432at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008429">
        <property type="entry name" value="CLPTM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21347">
        <property type="entry name" value="CLEFT LIP AND PALATE ASSOCIATED TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21347:SF0">
        <property type="entry name" value="LIPID SCRAMBLASE CLPTM1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05602">
        <property type="entry name" value="CLPTM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="435"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="532"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="560"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="624"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="671" mass="76807" checksum="7C9F1D81B23D2153" modified="2017-01-18" version="1">MESLIGLVARGSSSGGRLGKVFTYIRYLLLVIAVIXFIKKMFLTADPDDLKLMPDERMIPKPTTFGKKFAYRLMKAQFKSKGLALKDYGKSLERREYEKNLNLLPKGSKEVFLNHPKPNVDYQNITAWVYDADRKEKLTSYNDSVPTIEYIKYANSLDPAQLLIPAWSPSDTVDFSYYITAYPEFDIGSSSTFDAPVVHELLKDINLQNNHQISILPFVFDLPKDSQNLYMHAFMKKSKCKSIEFQDDDTCLISSNKTIKLTYTDPDTGKSYYHPLFQITLAYNNGDLLLSSVAEYFKPHLIVDDFTGKRDNSSGVATRYFPYIEYESFWQRKTELIEINDSKLSFEVEVNIGNVVALQAKLRVLDYFLQKFLSIPFTTVKSLQKFDFKFDSFKGCIVDNSQAMRYFCTSLVILQFVFGLLGFTSEAPINGGMNSTSHLLVAGLQFMILLYLLSQAQYTSVMVYFSTILASFSNVIKANELYDKKFYEKNELKPEKFKASKQLGEKTLIFQERYPKCLPFIEPEFETYRDDVAAPTLVICFGLLGAAYGIYNFFKDDSSVISLNASFLATLSYGVGILNDSFILYQNNKFQKIVPLSKELIVFKSALAVINIAFLCVAKMPIFHALYLISPTVIFFVYLYQTKHYKHIQETTEQTKPSKEIANEKEXKKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH89</accession>
    <name>A0A1E5RH89_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">GTPase MTG2, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1065</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH89">
        <property type="interactions" value="499"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH89"/>
    <dbReference type="InParanoid" id="A0A1E5RH89"/>
    <dbReference type="OrthoDB" id="3971242at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01898">
        <property type="entry name" value="Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.210.12:FF:000001">
        <property type="entry name" value="GTPase Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.210.12">
        <property type="entry name" value="GTP1/OBG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006169">
        <property type="entry name" value="GTP1_OBG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036726">
        <property type="entry name" value="GTP1_OBG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045086">
        <property type="entry name" value="OBG_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702">
        <property type="entry name" value="DEVELOPMENTALLY REGULATED GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702:SF31">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01018">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82051">
        <property type="entry name" value="Obg GTP-binding protein N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51883">
        <property type="entry name" value="OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Obg" evidence="5">
        <location>
            <begin position="118"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="OBG-type G" evidence="4">
        <location>
            <begin position="387"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ86244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="574" mass="64711" checksum="29677D4296407485" modified="2017-01-18" version="1">MFRLSKVLRYARKWNSLIPDNAPRALENEQWLQTLENGVGNLETLPQQGQHNMEIVSSLSSGTAXSPXDSRNFEKVESFRIISKPPETPTFRAETPVMNVTSLKYTNNDKFKQLKGNDEFVDVRIVRCKSGDGGDGFISFARDANRAVGPPDGGDGGMGGSIYIQTDPTMNTLSKIRHSYIAERGSNGAKDQKDGKRGRNVLIKVPVGTVVKCALDPKRVRRLLRDERTNGLGLNTREILQKYFVSVDCVGEYPSDRLPKYIQLYRNNSLRKKYKLPEANSTNSDGWIFKEKDEQYHREKDWFIKLDKKVKVHDFHESKDELVRDKFPMNGLDLNDKMERPVELLTGGNGGLGNMHFLTNMVRNPRFAKVGRVGIEQFFMFELKILADLGLVGFPNAGKSTILNKISKAKPRIGHWEFTTLNPTIGTVSTAFDQPTFTVADIPGIIEGASKDKGMGLEFLKHIERSTGLVFVLGMDKPNPLEDFKILLDELKDHGIKDVKRALIVCNKCDIDPDANHQKFMDIKAFVTEQQNSNWDIVPMSALNNENTDVLITKMNNLVQTARSENRARDTGIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH91</accession>
    <name>A0A1E5RH91_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glyoxylate reductase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1589</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RH91"/>
    <dbReference type="FunCoup" id="A0A1E5RH91">
        <property type="interactions" value="464"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH91"/>
    <dbReference type="InParanoid" id="A0A1E5RH91"/>
    <dbReference type="OrthoDB" id="9991913at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016618">
        <property type="term" value="F:hydroxypyruvate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12168">
        <property type="entry name" value="Mand_dh_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000026">
        <property type="entry name" value="Glyoxylate/hydroxypyruvate reductase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF257">
        <property type="entry name" value="GLYOXYLATE REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00670">
        <property type="entry name" value="D_2_HYDROXYACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="4">
        <location>
            <begin position="69"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="5">
        <location>
            <begin position="126"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ86278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="342" mass="37801" checksum="D6FC14A4F22FBFC2" modified="2017-01-18" version="1">MSKPIVLRLGDIRYAQAAWKKFGQEYDVIAIDSTSGYTREKFLKELQDPASKLSKVSVITRTFPSVQQTGLFDKELCDALPSSVKAICHNGAGYDQIQVEHFLERGIQVSNVPELVNNSTADTHVFLLLGALRNFKKGMLQLDAGNWNKDCAPGYDPCEKGVVGVLGLGGIGRCVVQRLKAFGFTKFLYHNRHRLQDPELENGCEYVSFDELLAKSDVLSINIPLNAKTRHIINADAFAKMKNGVVVVNTARGAVIDEQELINNLKSGKVASCGLDVFEHEPNVNKQNNELLSLSNCFALPHQGTNSVHTIKIMEEFVVENAESYLTSGKVKTIVPEMKGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH92</accession>
    <name>A0A1E5RH92_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein ANR2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1328</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH92">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RH92"/>
    <dbReference type="OrthoDB" id="2152680at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018626">
        <property type="entry name" value="LCHN/Anr2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053056">
        <property type="entry name" value="Lipid_Metab_Assoc_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28153:SF1">
        <property type="entry name" value="DUF4484 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28153">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09804">
        <property type="entry name" value="DENND11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ86257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="433" mass="49520" checksum="37C0315764D0DA39" modified="2017-01-18" version="1">MTVIGAFLTKFDVKKGNTLLWSSEFDPNTEINFENVEYLSLPSGLHDSKSGEDVVRFVLKSGDYTAQGPSLFPCVSIFYSNTKDLIIAGNKGVDRKDVRIYSLGVVCKPETSLFDAEHIEKYVPSLQQVMSLMNKDVPDPTILHNSLSKIEIDKDYQSKTPTKLFKNIGPLIFPLWKSCLLGERVLILVEDPSVKFEDMNYLVTYLFKISNYEPFENXNGIGMEHLAVGQRSKLLYNIGLFNYSNSKIDVYDHTKEHSGFVAYSPDIILRDMTTIYDKVLTIGSKGAVRMHDNKGEVIKSTFLDGSKVLKAKDFTNNANVDKRPWFQYMIDLLYTCFTMNNVPPPYSKYFDDIPVEPPINTETFIKENTSIHCMLIDLIRENRNTIKASEIMKLGLDGFSEQDFQLLTQLYSKLSKNEKRKLNVQWFDPNLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHA9</accession>
    <name>A0A1E5RHA9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal-binding activator 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHA9">
        <property type="interactions" value="469"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHA9"/>
    <dbReference type="OrthoDB" id="3971917at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="513" mass="56901" checksum="5E37CD163DE8A91D" modified="2017-01-18" version="1">MLVKVRSRGRPAPAGVRKVIMVDKSSQTDLRLLDQMKKESENGSRSSPMASESRSCCSSMNTSPEEQMKQETENCCSSVSSRSRSSRSRSSRSRSSKAESSNSGAVIEPALIEASEPALIDASDPVSAFTPSSWKSCCTQVRVKPDESTNLQQSETGSDKKTCRNDSSKDSKSCSGMSNQPILFLKAKHQSLGNLVNGQLNLIDNVNNNGDLKKTHTPTSQSYKKVLISETESLSKENSEEPQNSSVVHQSRSKEXTSHKKGXKQNEKDQSLFELFTQEGLFLSTSCSCSEDCECPNCLIHRKDEELNQYIEAHSMVPLSNMGYAELETNQSLSSNPKSSPASSINDYARNNSIQNMGTLIGHGSAFQQSGMTQGLNSWSKNPNLFAASNIGSATMCKFTEFDCIDINCRQHSFQIISLNTILWQGLMNTKLTKKTGIKFKNKIVCSKHWWDFLAVQIPHMAARDPASFDHFDIITWFTNILELYHAELLIDMGDYNHNIESMKHNWVIAGAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHB9</accession>
    <name>A0A1E5RHB9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein ECM30</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1213</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHB9">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHB9"/>
    <dbReference type="InParanoid" id="A0A1E5RHB9"/>
    <dbReference type="OrthoDB" id="432953at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005797">
        <property type="term" value="C:Golgi medial cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000138">
        <property type="term" value="C:Golgi trans cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026705">
        <property type="entry name" value="Hid-1/Ecm30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21575">
        <property type="entry name" value="PROTEIN HID1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21575:SF12">
        <property type="entry name" value="PROTEIN HID1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12722">
        <property type="entry name" value="Hid1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="614"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1111"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1175"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1387"/>
            <end position="1411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1439"/>
            <end position="1465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="626"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1111"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1135"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1177"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1387"/>
            <end position="1407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1597" mass="180922" checksum="39F1476A7E645247" modified="2017-01-18" version="1">MGNTDSKLNIYKQHILLLANADIVIPLYQQKRQSAAEMTFTTNGTGKNYEYTQTEQRTEGEEDEDIASIQSDGLNRFDEDEVGGEGRSPEKTLENPNADPYNDFFNCIVETNQYLSFADIDKSINNNEIKYIFYSNPTNFTNLLKFLCYKIIRVVNSISKTASSKSSGTNTNSNINHNFEFQIRTLLTCVRILTKIMPIFFELCYNDINKNHQLINEDSIFWEIDTVESNNFELNSVNEDDSFIKNSKLEENDESNIADALNSLKVDMTYKDTPVFNKKNSSTTTNIDGNSEDATENTNENGDGTTTVLGIRLLSSLLKSLFMEGFTLPLEKHSRKGYISFELWENGITSTNNDFVTLNPTLDANRLEILRLVEVLCSNCLYSESKIDNKFLASWCCCMPEYLSIYLLSSLTNVLLRSCHYLKNDYPQEYTIVNDIRSTEANHLQLLKTNTAFSNRITQTSTNGTYSHLNDFAETQINPIISNNNNGVPVSGNEKIYTLSDTEATQLRETFIKIATQMLTVYFQFYPNSLSSAGEKLLRGAMHDQKDWFLHSVAQRFFSTIQKEADFKKILVSFNKILKYPMNAVLESENNLFNLNAKKFNIFSYFKSGNNANDKENGHSGKLVGSNHSTNTTASTTSNNNNNNNNNNNNNNNNRNTNNKSDTNEQGSSQPNLPDFPENFNSVLLIFWEFMKSNKIFEKFIIEKYSNKLFMTLVYYLTNYSSDQRFRTITIPLVSQFALYMTSCPLFLFKSMHYIQSSYYAEKLPDSFKIIATSNISNTTYRDFAIIQLSRIALHDVQQNAPPKAHIYEMLYNLLLAIPSFLKEIVCSYPDGDIALXDLIKTEVVQNSNQYGSFMTGISYSASAQCVQLVYKSSNKQYLTSNANSNATMKEKSPTRPGLISAESNGSIATINENESVASFAQEVNSSAQETDENKLNLSAHCNHVINFLVNSKIVTVNNISKKPYLHTPAAKIDNLVLLIRNLSYSCINHYRDCRNLLFALTKYDKVMINLKQVLQEINVDIENQIEPLLLKDVVVPKNSKRLPYDLCVNTVPILTDDILYADDDFLNDFDPDNDNLLKSLTLRDSYKEDAYYTQGNPYVYFPGPEASFAQSLHSKQKRSSQQGSAARLDNSAPSSGLSSNHNLKSSSNNNGSQFIFQHADTRYQPDEQSNLVYHLTRSKRPHSLTNKKKKKLVMRSSDKLRQPILPTQTNHYWFLGEPFFEVLSKLVHILCAKFPRMSLLQAPQYHDXISQIAAKDKEVLGEIQTYLPFELKHTKHERPTLLKVNLNDGGVAQQWLHTLVWMDFFNLNSIEYQSNTEQMSEHSDLLELSKTNTLHSLNTSAESTHNTLHNSAKHSARKIVEEESLPPSSPMLEKWMSQNSLSRTNSNASFINNVGTNENXNESARSSLDFKKQDVLSRQASDKSKIGVASLNSQINLENKPSASSTSETSVRKLSSASSSNESQGFFSFGWKPSKNENLESQKKGLASSDTVALEGTARRPLIYTVDQEILNCLVWNNTGIAMFDIVSDKREEFSLMDMTSSLLNKFKLKNNVTESTDAHSKMCDEVASMNLRNPKIQANWTPRSSVSSISSQPYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHD0</accession>
    <name>A0A1E5RHD0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Acetolactate synthase</fullName>
            <ecNumber evidence="4 12">2.2.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g1463</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2 pyruvate + H(+) = (2S)-2-acetolactate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25249"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="2.2.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="12">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the TPP enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="4 12"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHD0"/>
    <dbReference type="FunCoup" id="A0A1E5RHD0">
        <property type="interactions" value="619"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHD0"/>
    <dbReference type="InParanoid" id="A0A1E5RHD0"/>
    <dbReference type="OrthoDB" id="16262at2759"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02015">
        <property type="entry name" value="TPP_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000008">
        <property type="entry name" value="Acetolactate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000007">
        <property type="entry name" value="Acetolactate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000053">
        <property type="entry name" value="Acetolactate synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012846">
        <property type="entry name" value="Acetolactate_synth_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039368">
        <property type="entry name" value="AHAS_TPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000399">
        <property type="entry name" value="TPP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00118">
        <property type="entry name" value="acolac_lg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968:SF13">
        <property type="entry name" value="ACETOLACTATE SYNTHASE CATALYTIC SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="THIAMINE PYROPHOSPHATE ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00187">
        <property type="entry name" value="TPP_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="16">
        <location>
            <begin position="98"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="14">
        <location>
            <begin position="293"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="15">
        <location>
            <begin position="502"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="49"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ86318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="694" mass="75374" checksum="65AEF67E437787DC" modified="2017-01-18" version="1">MMRQCISSSNATKRSFSKMIMRNCNTRSMMKTFPAVSVNTLRYNSTVASASTSTSTSASRPQPAPSFDQEDELNKTKNLQHDLASTNGMDSSFIGLSGGQIFEEMMRRHNVDTVFGYAGGAILPVYDAIYNSEAFNFVMPKHEQGAGHMAEGYARATGKPGVVLVTSGPGATNVVTPMADAYADGVPLIVFSGQVPTSAIGTDAFQEADIVGISRSCTKWNCMVKTIEELPRRINEAFEIATSGRPGPVLVDLPKDVTAGVLRRAIPTNTVLPSSTLSRLAGQKMNQYTLENIAKAAELINKAKTPVIYCGAGILNNPEGPKLLKELSDKCQIPVTTTLQSMGAFDQNDPKSLDMLGMHGTGAANLAMQNADLILAIGARFDDRVTGSLTKFAPVARQAALNGEGGIVHFDICAKNINKVVEATVPIEGDATRNLENLLPQCNKVAKRDAWFKKINAWKKEYPLAYNKETPGSKIKPQTVISELSRLSEEIYPNKDVIVTTGVGQHQMWAAQYWKWTKPRTFLSSGGLGTMGYGLPAAIGAQIGKPDAVVIDIDGDGSFNMTIGELSSAAQAGAPVKIVVLNNDEQGMVTQWQSLFYENRYSHTHHTNPDFIKVAEGMGLKALKLEDQTKTEETLREFLEYNKGPILLEVIVEPKVCVLPMVPGGKGLHEFISYDAKVEQDMAELRKKRTNGKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHF4</accession>
    <name>A0A1E5RHF4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">1,3-beta-glucanosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g1005</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Lipid-anchor</topology>
            <topology evidence="1 11">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glycosyl hydrolase 72 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHF4"/>
    <dbReference type="FunCoup" id="A0A1E5RHF4">
        <property type="interactions" value="192"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHF4"/>
    <dbReference type="InParanoid" id="A0A1E5RHF4"/>
    <dbReference type="OrthoDB" id="421038at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031982">
        <property type="term" value="C:vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042124">
        <property type="term" value="F:1,3-beta-glucanosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071970">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1040:FF:000005">
        <property type="entry name" value="1,3-beta-glucanosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000038">
        <property type="entry name" value="1,3-beta-glucanosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004886">
        <property type="entry name" value="Glucanosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468:SF2">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03198">
        <property type="entry name" value="Glyco_hydro_72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 11" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9 11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="11 14" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009028106" description="1,3-beta-glucanosyltransferase" evidence="11">
        <location>
            <begin position="25"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="13">
        <location>
            <begin position="379"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="501"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ86331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="578" mass="61541" checksum="2040DA5E43CCA6C7" modified="2017-01-18" version="1">MLFNVLVSTLALGLTSVFTRFANAEDLPAITVKGNKFFFSNNGSQFYMRGVAYQADSANATDGSTIIDPLADADTCKRDIPYLQELSTNVIRVYALNTSQDHTECMEALNDAGIYVIADLAIPGESINRDSPAWTTDLYERYTSVVDLFHNYTNVLGFFAGNEVSNAYNNSEASAFVKAALRDTKAYIKAKDYRSIPVGYSSNDDSITRVAMADYFACGDSDVSADFYGINMYEWCGSSSFSKSGYEDRTKEFSNYTIPLFFSEYGCNAVQPRKFTEVGTLYSDDMTDVWSGGIVYMYFEESNNYGLVSVDGDDVSTLEDFGYYSSEINSITPSSANSASYSPTSTSMSCPATNSHWLAATKLPPTPDRELCECLYDSLSCIVSSDADDTAYSDLYSYVCDQIDCSGITANGTTGKYGAYSFCDDRTKLSFVLNLYYESVGDSSACDFSGSASLQDATTQSGCKAALTQIGSVGTGSASATASFTGSFAYDDLSGTVNPSATGSSKKSSSSSSNAKSSSAASSDSSSGSKSASASSSASSSKASKSSAAVNSNINLNQVLLTSFATIFAVAGVSFVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHF8</accession>
    <name>A0A1E5RHF8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2',3'-cyclic-nucleotide 3'-phosphodiesterase</fullName>
            <ecNumber evidence="3">3.1.4.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1073</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the metabolism of ADP-ribose 1',2'-cyclic phosphate which is produced as a consequence of tRNA splicing.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 2H phosphoesterase superfamily. CPD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.37" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHF8"/>
    <dbReference type="FunCoup" id="A0A1E5RHF8">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHF8"/>
    <dbReference type="InParanoid" id="A0A1E5RHF8"/>
    <dbReference type="OrthoDB" id="514292at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004113">
        <property type="term" value="F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009187">
        <property type="term" value="P:cyclic nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012386">
        <property type="entry name" value="Cyclic-nucl_3Pdiesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28141">
        <property type="entry name" value="2',3'-CYCLIC-NUCLEOTIDE 3'-PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28141:SF1">
        <property type="entry name" value="2',3'-CYCLIC-NUCLEOTIDE 3'-PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07823">
        <property type="entry name" value="CPDase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="LigT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="82"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="82"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="99"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ86296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="255" mass="27605" checksum="1EBBDDE159D916BC" modified="2017-01-18" version="1">MELSNLMLSLQSLFPDAPDMHTPHITITSGLNVQDSKKILSTVVLALKSTRNKARERSSTTGSVISAGSLSSVSATARDSTYFSSTSGSTSRNGAASTLQKNGGLTTAPSVTHADGSGLSFQRLSLGSTFFTRVKLDIAKENNKVLLSLVKLIRDMYVSKQQNNAEYVINEYQPHVSLVYSARIPSRAEYLSIVNRIEDAMDCEIDLANGIIADDTLEPSVSLFSNAWSMINGTFMVVNCVGPIEEWEVLDSVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHG9</accession>
    <name>A0A1E5RHG9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein ECM19</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1275</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHG9"/>
    <dbReference type="FunCoup" id="A0A1E5RHG9">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHG9"/>
    <dbReference type="OrthoDB" id="4080273at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="95" mass="10585" checksum="271A93A5446331FC" modified="2017-01-18" version="1">MARLRAFDLSVFGVVIIAGIYGGTKFFEPIVIDALKKENALRTDIPIPEYSDDGIPVENEKSLANLKTELNEIYEQSRRERGLEPKSLGSSSNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHP2</accession>
    <name>A0A1E5RHP2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcription factor SFP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1259</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHP2">
        <property type="interactions" value="2222"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHP2"/>
    <dbReference type="InParanoid" id="A0A1E5RHP2"/>
    <dbReference type="OrthoDB" id="3269380at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051580">
        <property type="entry name" value="ZnF-Chromatin_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23057">
        <property type="entry name" value="JUXTAPOSED WITH ANOTHER ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23057:SF0">
        <property type="entry name" value="JUXTAPOSED WITH ANOTHER ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <sequence length="952" mass="104551" checksum="6FE02D20A64120BB" modified="2017-01-18" version="1">MLIDQTATTETAALSPAITSPNNNQGSKDLLRSXNKSSPXLLSNMANGRENSIRSASSPSPSSSSPSSPSSTHSSSTASSSTSTTYTKPSLTAISPNTEMPITNDQRTPATVDSNSNSTANSKIANQDKQTLLLDDVAKTRRASLEQFQGISGVSWKKLNIASWLKDDVMFNKLNSAINLRLQQQKQENLRKENKQQPENKIESQEANYSTLSNKENGKDFSKKNLSVQASYLPTLEKQYCKDYACCGLLLPSLHDLLDHYEDVHTTSSLSRPLPNYNNFHLNNAHSTITSLRSNYEGNKVTNYSKKRSSSSVETPDVKAFALKEKLLNTATKKSKLNRASSTPGSSHTEHEFAKPILSSTGKNFSLSNKNAKISQQSCQPENLKSSHDTPDHKHEKPSFTENGSTSSISKNKQSLVHEQAALKGNLGNNDFSKMQSNTLANKDGVLGTNAKDVGKNTKDTNFNISGLRGEEKKVQSKQKTQDGESDTENXKHQTQNDKDSDRSKYLVNDAYEETKESNNNHNASHNGSNSNSAVSRANSDMTKMAMSTTEDEKILVNTSQLHLDHPHHAHIFKRPSSVSPLTVSEKAKDSLLHDINADKHAGFHTAEANYESEITSMSNPESATSSFHGNYGDHAKIAHENILSXNGITSEDRSHXKSISDRTLDVAPATIYMKTVSPLARAASISMPIASTTNKDTKAGLDTKSIPGTPKHRSSVVSMASPASVPVVRTTSLAFPSKTSLLKKSHDESLQEFASSTLPGMTKPASALKIRAGADHDELVRAHSQQRSRTSSITSSMRSIHSDDEDFAKYTSPQTHQFQNNFSESSDAPDVSKSFININELVGNRGFANEQARRLYLMELEENKPFKCPVIGCGKMYKNQNGLKYHRAHGHQGQKLYENADGTFSIIDPASNQPYPDGMEFETDKPYRCDVCAKRYKNLNGLKYHRAHSTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHW0</accession>
    <name>A0A1E5RHW0_9ASCO</name>
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        <submittedName>
            <fullName evidence="2">Vacuolar protein-sorting-associated protein 24</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1305</name>
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    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="term" value="P:vacuolar transport"/>
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        <property type="project" value="InterPro"/>
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        </location>
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        </source>
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        <source>
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    <sequence length="242" mass="28130" checksum="10D70F25CEC7B1D3" modified="2017-01-18" version="1">MDYFKKAIWGPDPKEQQRAVKRVIRKNIRECDKSLNELSSLKTKTERLIKQCVKKNETKKIRIYCKELYKINKQYERMYISKTNIESISMKVNEALNLQKISQSMVQSASIMNEINNLIHIPALRGTVMEMEKELMKSGLIDEMIDDTLDMGEDEFEEEDEEVEEEINKILQKYTVQEKPKPVLEKENEQSARLPSIPSAANPVXAEPEKQDEGRLVPEAEVDQEADDLINAMRERLKALQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
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            <taxon>Saccharomycetes</taxon>
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        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
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                <person name="Lee D."/>
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            </authorList>
            <dbReference type="PubMed" id="27856586"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EFG1/PHD1/stuA family.</text>
    </comment>
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        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA-binding domain of Mlu1-box binding protein MBP1"/>
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    </dbReference>
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        <property type="entry name" value="HTH_APSES"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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            <end position="513"/>
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        <location>
            <begin position="987"/>
            <end position="1009"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1009" mass="110846" checksum="A32461EF69014A69" modified="2017-01-18" version="1">MYQAYHNPMIQQQDVAGNGSNNNNNNNASGNSNIPNGMNNPDPNRSYNQQYMSNNQNVVQQSVPQQMPQQSMPQHYASQQVYQQVPQNYASQQVYQQVPQQMPQQMPQQMSQQMSQQMPQQMSQQMSQQMSQQMPQQMPQQMSQQMVQEQVPQQYTGQQQYSNQQPYQVQQGYATYANNDSYRTPNKELNRVTNNNFDNNNSNSNTGVRLPPLIAVQENSMAYSNDTRNDNTVTNIVNNANNMQGNASIRSPPXMPVLPNAHYITKMSHSRSGTLEGYPHTPNSTAGVPASTSTSTNTTATATTTTNNNNNNTMNGNTNMNTTITTQNNMHGNIPRQSVLQPLIVQPMQRVHNVQTVQGVPENATVVQGSTAGNSPSAMSTYSTTSTVPTQTNENPTPSSFLPIDPKVITTIWEDENTVCYQVEANKVSVVRRADNDMINGTKLLNVTKMTRGRRDGILKSEKVRHVVKIGSMNLKGVWIPFERAYLIAKREKIVDLLFPLFVKDIQNIIFDPKLNRQSLTQSINQHLQKQNIQQGHANQSNGTNTSNNNIXSTNTLQHNAXSAVSTPSGPRTNEKTFVENEVPESGNPTISNVNTQGNMYYTNQNYQSPPYMDSNTGSRITPPGTNTRSNPHSLLRPNHTGESITSNVSSTNTYKTTHSNPLFLHPINVPHHSSVPSISSVSSTSSATTAPNNFAYHHGLHSVYPQQQAHQQQQAHQQQQQQQESSHQGSATPQTPYLYNQQQQDRQQHTTPAMTNSNMILNPSSATTIISTPRTNALSFPPGANAQQPQQYQQLPPPTLPVLSAPITQIKTDRPQNTLGGMSPPQLLPSLPIPGQYYTAGISLGQSMSQPMSQHGGNTQGDHTLHQSHSMHSHPFYNPVPSSSSASGAPQSANTVTSGTASAAPHIMIPPMNVPSVSAQQPQQPQQPQQPQQHPQVLHHQTNSRSQFSSFSDTSGSRSSTLSGDPFIKVQQATSNLSATSALSQPQSLGVPQNTGLPFGSSNMPSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI48</accession>
    <name>A0A1E5RI48_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1295</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RI48"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097196">
        <property type="term" value="C:Shu complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031779">
        <property type="entry name" value="Psy3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16836">
        <property type="entry name" value="PSY3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ86545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="297" mass="34426" checksum="03C364730B70E065" modified="2017-01-18" version="1">MDILNQCKLYPLSQFYKPCMINWANLPSDSPIQVGEKKEESHLQNEQQLKCRFFKELSNXXELAQFNKVNKLAHSKFNGNVHLNVQTLINNNYTEFVLKHLLYSMMLIFAEDMADIQREKECSDEDLAETDKYVVILIIDVASSWHTICPAMTFDKDTGITIIRQRFETSIMSELSQLLSFLSNTVLDISKFKLYLQKNFNIPDYYQLGAIVIDNLSYFNFDFSTAASPCSLSHAITQMFNNSTYHHFFSYNFXVKLPLPGEADNTESLNNLMQLLHNIRDRYGALAITVSFDNRFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIK5</accession>
    <name>A0A1E5RIK5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Fluconazole resistance protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1398</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RIK5">
        <property type="interactions" value="308"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RIK5"/>
    <dbReference type="InParanoid" id="A0A1E5RIK5"/>
    <dbReference type="OrthoDB" id="4940293at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050936">
        <property type="entry name" value="AP-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621:SF8">
        <property type="entry name" value="AP-1-LIKE TRANSCRIPTION FACTOR YAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="4">
        <location>
            <begin position="133"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ86413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="322" mass="36188" checksum="55EE1F1444B54AC4" modified="2017-01-18" version="1">MESSFFSTDGTDFSKSHNFDPYEDQNNFFAKNYHDVYSVPAPSGASCLTQPENXLPFDTNAIGTEHKNACSYSSGTSDILDNRGFVGNNGITSTSSLATVSPFGGTVKDEDCQLLNKHNSLINETPGELEFSNETGDKKKQQNRAAQRAFRQRKEAKLKELELKLTQTENDNQKLQEELESLRKQTMEMSMENKLLLNRQQNHVSSFPGTNMAGNTASRAVKYNIPTKSEFQDFIVKDQPYHQGKLDSLTYTLPEGETLMTISATWDFLREMLDHTEATQGKTFDLYEVMKDLKGNEKCHGHGAAYKKDFVVSVAEAHLTDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RMZ4</accession>
    <name>A0A1E5RMZ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Protein EAR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g897</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Components of the endosome-vacuole trafficking pathway that regulates nutrient transport. May be involved in processes which determine whether plasma membrane proteins are degraded or routed to the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SSH4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RMZ4"/>
    <dbReference type="FunCoup" id="A0A1E5RMZ4">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RMZ4"/>
    <dbReference type="InParanoid" id="A0A1E5RMZ4"/>
    <dbReference type="OrthoDB" id="258495at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000065">
        <property type="entry name" value="Ear1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="16">
        <location>
            <begin position="145"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="448"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="465"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ88261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="517" mass="58862" checksum="AF197DCBAA95EF30" modified="2017-01-18" version="1">MNMNDGTNHDMAPPMELDLIDFFIFLFYTFGVMFFSLLCLVMMYVIVKNIIMRLFGLVVANSGDESSTDHTRTISLLDPRYVDQRLNDQTHGSRGQRYDLTKYYDDPQDVELELEKLPTEEQFYYKQGEEYMKHNPPFLLLNDNDFDPIMTEQTMTYIEEEGAGAWEFQPDQNLPNDTILVDNKTDLRFLNYGYEASIMTTLPIPYKNRVYYYECKIYEMNTADKNQEEVDTADENLQTSMNEDEVISIGFASNPYPSFRLPGKHHHSVAYESNGGRRMNSSFPLSPELQHLLPKIQKGDVMGIGYRVRSGTVFFTHNGKKVKEVEVGGHIRKWRLKYLYPIVGANIPCRVSCNFGTSGFVFVEANVKKWRFASMNGLKMPPPAYGNVGSDAILEEGHSNVTEGSIYMANRGSFSGGQTSKNSGNLSAEHLRLLDDDRDDAEGEMANQYHDYEAENSDSNASDMTNEDTDNDADELDPPPPDFNFSNPVPTLQNDAISLNTLAPQNPPEYFSDLERN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN47</accession>
    <name>A0A1E5RN47_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="7">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g875</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN47"/>
    <dbReference type="FunCoup" id="A0A1E5RN47">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN47"/>
    <dbReference type="InParanoid" id="A0A1E5RN47"/>
    <dbReference type="OrthoDB" id="2020758at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02257">
        <property type="entry name" value="Peptidase_C19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF888">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="10">
        <location>
            <begin position="101"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="135"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="297"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="591"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="846"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="140"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="156"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="597"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="640"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="671"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="683"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="701"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="713"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="724"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ88305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="869" mass="99057" checksum="6201C9E82103F211" modified="2017-01-18" version="1">MNNFWESQRNKIPHYNPLDLFAKTTNTSVSSVYSLSAIVLILSLYYINPLQFRKHLKNLMSALQNHSTDYLTSKFRNSRIGRTTYTNALSSIVINEGGYVGGLVNEGNSCFMNSVLQSLASSKELMEFLDERIINSQEPTESDEEEDDDDDQDGEQQSKEDDVTSDVDTIKQEHLEENTKSKENESKSSTKKVVKRKYGKMKKKLNKFKDEQLLGKSDSSERKTGEADVTFSIALKDLLDKLNAKHYNSRPNFRTNKLLRTMTKKPNSNVILGYDQEDAQEFFQTLLNELEKNVKSVAAASQGSEDSSASSTSGSNGTPEPVSIDSIPENALEEQEEDLDRVGTVYIPTSQIDPNIKDTKLYTPFKLVTPLDGITCERIGCLECGENGGIRYSIFSGLSLNLPGEKFGVTLKLSELMNEWSKPEIIEGVNCNRCGLLAVKDYLLEEIKKKSGTLPDKLLNAYKDRLMQVEELLSKSVIDDEDYQKFKISNMVQKSSKSKQILLSRPPALLAVHINRSCFDPRTYTIRKNNSRVVFKKKLNLLPWICKPDEINLDARLPMSKKHEGSKQQFEISSSEDEKVEKYYKKLHKTFEREYQDSDEEDDDGDEDEEDGDDDEQKYNHKYNSLNGNHHGNRAFPDSSDSDSDDDDDIESEIFEKPVDSLGNTISSSDESADVEDVDDDNDRLVALDDLKNQTSGSEARFGEEKHKRLQNEQQEQQAAGGDLSEEEEEEEEADMVPLPTLKSMSTNVPDGPFSYNLRSVIVHYGTHNYGHYIAFRRFRGCWWRISDENAYVVDEKEVLSTPGVFMLFYEYDYDPETGMLKDDLETARLERELEIELEKEQKIHFQMQDSNKQNLDKQETADATETQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN49</accession>
    <name>A0A1E5RN49_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">60S ribosomal protein L16-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g794</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN49"/>
    <dbReference type="FunCoup" id="A0A1E5RN49">
        <property type="interactions" value="1079"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN49"/>
    <dbReference type="InParanoid" id="A0A1E5RN49"/>
    <dbReference type="OrthoDB" id="1882297at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1180.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_uL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_uL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023563">
        <property type="entry name" value="Ribosomal_uL13_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005755">
        <property type="entry name" value="Ribosomal_uL13_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_uL13_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01077">
        <property type="entry name" value="L13_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00783">
        <property type="entry name" value="RIBOSOMAL_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ88294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="208" mass="23758" checksum="63ADBB3A38BBFA39" modified="2017-01-18" version="1">MKRGQFTLRQNKNKHEFQTGKGHLLGRLASTVAKQLLNGQKIVVVRAEALNVSGELFRNKLKYHDYLRKATRFNKTRGPFHFRAPSRIFYKAVRGMISHKTAQGKAALERLKVFEGVPPPYDKKKRVVVPQALRILRLKQGRKYTTLAKLSSSVGWKYEDVVAKLEEKRKVRAAEYYAKRRAYNKKAIVANASVAESEVAQKLASLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN58</accession>
    <name>A0A1E5RN58_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Aromatic amino acid aminotransferase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g650</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN58"/>
    <dbReference type="FunCoup" id="A0A1E5RN58">
        <property type="interactions" value="172"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN58"/>
    <dbReference type="InParanoid" id="A0A1E5RN58"/>
    <dbReference type="OrthoDB" id="691673at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047536">
        <property type="term" value="F:2-aminoadipate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008793">
        <property type="term" value="F:aromatic-amino-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009074">
        <property type="term" value="P:aromatic amino acid family catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050859">
        <property type="entry name" value="Class-I_PLP-dep_aminotransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790:SF2">
        <property type="entry name" value="AROMATIC AMINO ACID AMINOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="6">
        <location>
            <begin position="104"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="526" mass="60031" checksum="1177832B9470E36A" modified="2017-01-18" version="1">MTQLSYQDLEALYGPLVSKYDGERNFVSFTAVGELPRDLKIKNKDTVWLAGGTPYESMFPVQEVDLKIRENPGSLDDTTPLSNKCSTTKVQMYEPQGSAKACLSTAFQYHDAKGLPELLNLTKQIISRMNKPQYENWDTVVTPGSSDSLSKICQTLLDSDSVILVEEFTYPNIVHWAKFAHSEVVRIKLNVTADRKLQGIDVEALRDLLANWVEYHPGKPKPKCMYTIATGHNPTGLTQSTESRQAIYEICCEHNIIIIEDDPYGYLNMTPYDQANPMKNPYIEESYGVEHYCKKILSPSYMSMDTEGRVLRCETFSKVAAPGLRLAFVAGNQFLISKLLKMHYFTTRGTSGASQAIVTNMLVDMGEQYQAQHPHEKAPLIDGWVNWCMKVAAEYTHRRNYMFMYMYEQNVFKKNLVSFIEPSCGMFVTLVINFKPEWNISETKRIMDYLYCCLTEEGCSAVLGYKMVVDRDYSMERANFLRFTFAKARDPNVFKIGAERINAAVERLFEEYNVNEQFTLGPKGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN60</accession>
    <name>A0A1E5RN60_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10">Peroxisomal membrane protein PEX14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 10">Peroxin-14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g883</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the PEX13-PEX14 docking complex, a translocon channel that specifically mediates the import of peroxisomal cargo proteins bound to PEX5 receptor. The PEX13-PEX14 docking complex forms a large import pore which can be opened to a diameter of about 9 nm. Mechanistically, PEX5 receptor along with cargo proteins associates with the PEX14 subunit of the PEX13-PEX14 docking complex in the cytosol, leading to the insertion of the receptor into the organelle membrane with the concomitant translocation of the cargo into the peroxisome matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9 10">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the peroxin-14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN60"/>
    <dbReference type="FunCoup" id="A0A1E5RN60">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN60"/>
    <dbReference type="InParanoid" id="A0A1E5RN60"/>
    <dbReference type="OrthoDB" id="5549158at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025655">
        <property type="entry name" value="PEX14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006785">
        <property type="entry name" value="Pex14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23058">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23058:SF0">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04695">
        <property type="entry name" value="Pex14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="3 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peroxisome membrane anchor protein Pex14p N-terminal" evidence="13">
        <location>
            <begin position="7"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="40"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="291"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="136"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="326"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="342"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ88322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="352" mass="39809" checksum="ACDD6E34014C1B27" modified="2017-01-18" version="1">MSDNVYQSAVEFLRDESIKDAPLDKKKEFLKSKGLSEDQIGQALLESAQPNHGLPNESNQSSNENVEKLGQNSYLSEKPKYEYYAVPPPLPRRDWKDYLIMATSTAGVLYGCYSLTKNHIIPRIFPKERSQLDQDKKDIDSKFSKLDETLEKLESQEQALKEKEEEKIKQLEDTLKSLTETLEETESSKRKMDTEFRLMKAELLSLQNTIDKFFLDSAKMNDMDNIAKEINSLKLLMEKSASLPDSTIMTPADNHTPVIDANNIANTPNQERKNKIASPSSVPSASDILSKMNLKPKGSTPVTKPLLNPVDHETKEETPAWLNARTENESNKSKIEIPEWQKNLTSASNNSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNH2</accession>
    <name>A0A1E5RNH2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Autophagy protein 16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g941</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNH2"/>
    <dbReference type="InParanoid" id="A0A1E5RNH2"/>
    <dbReference type="OrthoDB" id="8949486at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22887">
        <property type="entry name" value="Atg16_CCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="172"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="42"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="83"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="243" mass="27135" checksum="B7CDB84657CDDA69" modified="2017-01-18" version="1">MSSERDSGPENATISPKRTEKTLFPGAPLPEQISKPNIQPHESFKKDHSVTQEEKTSLKANSVSKSDAENEADGVDNGDLPENSDSAASDNATIPANSAPANYSEKIMESQLNDSEIINLLNTRDAISHNFSQLFVSQSELASYSLPARNRKSTKGDTGFLEQILGKCKAQLLEKERQNTSLNEVIETQKTEIRHLQDEMLSMNIENNLLHQELLKRQTEYDKLVERWMAKAQQDADAMNANF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNL0</accession>
    <name>A0A1E5RNL0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">DNA cross-link repair protein PSO2/SNM1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g961</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNL0"/>
    <dbReference type="FunCoup" id="A0A1E5RNL0">
        <property type="interactions" value="217"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNL0"/>
    <dbReference type="InParanoid" id="A0A1E5RNL0"/>
    <dbReference type="OrthoDB" id="262529at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16273">
        <property type="entry name" value="SNM1A-1C-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011084">
        <property type="entry name" value="DRMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240:SF6">
        <property type="entry name" value="DNA CROSS-LINK REPAIR 1A PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240">
        <property type="entry name" value="DNA CROSS-LINK REPAIR PROTEIN PSO2/SNM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07522">
        <property type="entry name" value="DRMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA repair metallo-beta-lactamase" evidence="8">
        <location>
            <begin position="541"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ88476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="690" mass="79822" checksum="ABF222F6FDC6DFC5" modified="2017-01-18" version="1">MMLFGENSRKKATQEETSSKKKLSIVDLLAKRNINFAFANQKQSKSSTLSNEDNPICIVSSDEELQNDADEDLSQSKKELVTYRQEIVDFTERTQIDLNGAALQSDQEVSVFTDAKETGKETQDAMEVTCAKQDGIDVLQNPPATTQLQKEEIESSDLESERATVLEATNITERQSPYKVELRTVDIIRQEIFNKKLNSTVTKSKETVPTKPKRRRATIPPPDHKVLFFTEEDQVAVDGFQYTCPEIEHHFLSHFHGDHYCGISKTWFNIHPSSKIYCSEITRDLMIYKFNIYEIELQERIISIPLGKYVFLSRNLRVLFFDANHCPGAILMHFEYGQQTENCLSEANSQKKKIVFAPEMKYLHTGDFRFDLETMTELLEHDYDKVYLDTTFLNYRYKFPNKNQLVESLSAFVNKNIIEKFKSKETYQNQKTLDFFMLRNVSKNNAHNKLKVCILVGAYNIGKENLIIGLCKKLGNCTLVIDDNTLRSQVDLEYEHCIRTNDYAETIVQALQPGDAASTTTPNDIVVCIVPTRDLGSITTKEMKDKYCRKVSHRFPNLQVVSIIPSGWNYTSRWSNGDDKNVEVFTPFDQSSCEQVLAHYMGIPFPFDIDSLLNVSRQKMIKFDEKYNVTTIKIPYSDHSSFLNLCEFGVYVRWKEMIPTVNLHQDKAYFDYWFNLWHMNQEKKIFAGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNP0</accession>
    <name>A0A1E5RNP0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g768</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNP0"/>
    <dbReference type="InParanoid" id="A0A1E5RNP0"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="354"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="464" mass="51855" checksum="71AECDB2DDE62FF3" modified="2017-01-18" version="1">MTTNDYYRSDFEFISSEDELDTARHTGGEYVDCLESSTSDCCYSEDESLSFTYESVETSDGNESQSEASVLFVDEITYFSDLSETPTDSDRVLNNSSSSLDFFTEVFARDSASNGSICLNGSATESAIGSAEQSSLECFDLEIVIDRRVESDHDYEFETLVESSKSENDDFKLQEREAQNVFDQNNFANDFHISQLSYGGVKESKKVDLSSRSGNTKDLSSNKENICSKRRLSSRVPSAAVYVFKFLLNIASFLLKISFYTGVMTSSSILCNKCINILCQSQKDSFPDVKNVFSYMSNFSDESVGKLGAIFAISNIVLQKTISTALKCCNCATTTTTTTTATALTKDSRDSQTLKGFLLCAFSVFTMFSYFASVDLINSPNSTLVWPVFNIALSVVFGGGNYLSEAILYYSWVFISTNKHFESTSLINASFEYWETWCNKLNFHFGLFFVFAKLSKYVCERIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNR9</accession>
    <name>A0A1E5RNR9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cytosolic Fe-S cluster assembly factor NAR1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Cytosolic Fe-S cluster assembly factor nar1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Nuclear architecture-related protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g577</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NARF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNR9"/>
    <dbReference type="FunCoup" id="A0A1E5RNR9">
        <property type="interactions" value="376"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNR9"/>
    <dbReference type="InParanoid" id="A0A1E5RNR9"/>
    <dbReference type="OrthoDB" id="10253113at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.950.10">
        <property type="entry name" value="Fe-only Hydrogenase (Larger Subunit), Chain L, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050340">
        <property type="entry name" value="Cytosolic_Fe-S_CAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009016">
        <property type="entry name" value="Fe_hydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004108">
        <property type="entry name" value="Fe_hydrogenase_lsu_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11615">
        <property type="entry name" value="NITRATE, FORMATE, IRON DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02906">
        <property type="entry name" value="Fe_hyd_lg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53920">
        <property type="entry name" value="Fe-only hydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Iron hydrogenase large subunit C-terminal" evidence="8">
        <location>
            <begin position="110"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="437"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="444"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ88534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="574" mass="64743" checksum="5B62AB1958926B8F" modified="2017-01-18" version="1">MSRLLSEDVLNDYIQPSQVCIKPTEIVKGQPSTPAAEKNEQFGEVEIQVGKEEESVGLDKVSITLDDCLACSGCITSSEEIILEKQSYQVFLNDYKESLSADSQKSGVELMISISPQCRLSLSEYYQIKNVMKFDIALMRFLSDKESVFENHCKYVVSTQVGRNIVIEETNKRLFTNNELSMNKSKLPQLSAVCPGVVMYIEKTHPKLVDYFLDIKSPVSITGNLVKAVRKDSMSFICNNDGNVQPKTYHLSIMPCFDKKLEAVKTGKDIECVITPKEFVNMLVDLNIDFTKYIPLENSKDELMHEYKLLAKKFNPFDSIDPLMSWEINEGSSSGGFAYQYILYQYEKNKELHGQNLQIEVVNGKNSDMREYRLVIKESNVFNDQMNKTQSTQYQILHRSSEVYGFRNIQNVIRKYEEKLNPKDSKLTKKKRGLVLRKNRKQENSLGEKTSGTEVSNAATVANSNPPCDPLNTEFIEIMACPGGCINGGGLVVPSADQNNGGNAKRKTQQISQLNEMYHSQIPVTKVQQHLGADFESQHSVDFKYTFANLENATKSAETLHVAPDPSASLFSAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNT3</accession>
    <name>A0A1E5RNT3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Acyl-coenzyme A thioesterase 9, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g776</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acyl coenzyme A hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNT3"/>
    <dbReference type="STRING" id="56408.A0A1E5RNT3"/>
    <dbReference type="InParanoid" id="A0A1E5RNT3"/>
    <dbReference type="OrthoDB" id="331699at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03442">
        <property type="entry name" value="BFIT_BACH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000032">
        <property type="entry name" value="Acyl-CoA thioester hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12655">
        <property type="entry name" value="ACYL-COA THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12655:SF0">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="6">
        <location>
            <begin position="143"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="HotDog ACOT-type" evidence="6">
        <location>
            <begin position="370"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="556" mass="62876" checksum="44A394E526AD2093" modified="2017-01-18" version="1">MLRTFNVPRFQTLNKSINQSKKLFSTTKIQNYQKSMVIMNKRPLHDDPHVTDNTPGTDNAATQPDALTTVMTEQYDRQRLFDQSNCANVNKATSPEEAPKDLKFKATWLNAIKERQKQLSHGKIIDSYLYNNVKSTEIKDKTRKDSFSYLYLPFKEDPLIADFYVNAGGRIRMGLVFQDLDALAARIAYRHCSPSEPIIVTASVDRIFMLQHMDSITDANLVLSGSVVWTGRSSMEITVKATAVPKNVSVPKETTTNADGTSTEEPHITEEFLNNPEFQTVLMSSFTFVARNPETHKSLAINKLLPINEKEWSDYKRAESHNVAKKLRAKNENLKQNASPTAEESQIIHNLYKANESIQGLSTPFVKMMKDCCFKSTMFVQPQFRNRHSFQCFGGALMKWTFELAYCTTTSVSHGLPRFLSLDATTFKAPVPIGSILHLDSKVVYSEHISKSGNPNSNSAIEKFLPLAQNDPKNFTSDGTVIQVKVDTTVQELQSDKKYSSGSFIYSFFVPKQDKDAEDFTVLPESYSDYIEYIEARRRTADSANYGENAKRLAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNV7</accession>
    <name>A0A1E5RNV7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein URE2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g586</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNV7"/>
    <dbReference type="FunCoup" id="A0A1E5RNV7">
        <property type="interactions" value="705"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNV7"/>
    <dbReference type="InParanoid" id="A0A1E5RNV7"/>
    <dbReference type="OrthoDB" id="422574at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10293">
        <property type="entry name" value="GST_C_Ure2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03048">
        <property type="entry name" value="GST_N_Ure2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000034">
        <property type="entry name" value="Transcriptional regulator URE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017298">
        <property type="entry name" value="Ure2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF3">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR URE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037861">
        <property type="entry name" value="Prion_URE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="5">
        <location>
            <begin position="200"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="6">
        <location>
            <begin position="293"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="83"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="94"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="130"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="252"/>
            <end position="253"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="268"/>
            <end position="269"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR037861-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="442" mass="49570" checksum="3784A8E309971E0B" modified="2017-01-18" version="1">MDPPCKIKSNHHFAKNNDTMDNTLGLNDNGNMNTSDNNVPSNMDSASNTNVSNLSSALKSVNLSYGNGTTGNQGNGDFEVIAQQQQQQQQQQQGANSSSVSQNGASTLSNSLQQQARMQHYNARQPSRLQQQYSQQQQQQQQMQQVPGQSTQQQPQQQQQQQQGQALPFSNHPASADINNKNATVDCSRISKLFATQPMDGYTLFSHRSAPNGFKVAIVLSELNMPYRTFFLDFSKGEHRCPEFIAVNPNARVPALIDHAMDNLPMWESGAIILHLVNKFANQTGQPLLWADNVQEQTQISSWLFFQTSGHAPMIGQALHFKYFHTNKIQSAVDRYTDEVRRVYGVVEMALAEKREALIMDLDTENAAAYSAGTTPLSQSRFFDYPVWLVGDKITVADLSFVPWNNVVDRIGINIKVEFPEVYKWTKHMMRRPAVIKALRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNZ3</accession>
    <name>A0A1E5RNZ3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">U6 snRNA-associated Sm-like protein LSm7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g756</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNZ3"/>
    <dbReference type="FunCoup" id="A0A1E5RNZ3">
        <property type="interactions" value="804"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNZ3"/>
    <dbReference type="InParanoid" id="A0A1E5RNZ3"/>
    <dbReference type="OrthoDB" id="274944at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097526">
        <property type="term" value="C:spliceosomal tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044641">
        <property type="entry name" value="Lsm7/SmG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553:SF5">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="4">
        <location>
            <begin position="54"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="21"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ88600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="138" mass="15414" checksum="3933DA7D14118900" modified="2017-01-18" version="1">MSIQHLQDQQEEEIAAARETQVLPPLTQQQQQKSAEPKHKEKNKSKKLQGPKRDQILDLEKYKDQSVRVQLAGNITVTGVLKSYDAAMNMILDDAVEYVADELDLSKLLRKRTLGVCVVRGTLLVLVSPNYAALASSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP28</accession>
    <name>A0A1E5RP28_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Altered inheritance of mitochondria protein 19, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g865</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP28"/>
    <dbReference type="FunCoup" id="A0A1E5RP28">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RP28"/>
    <dbReference type="OrthoDB" id="5554402at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019419">
        <property type="entry name" value="AIM19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28177">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 19, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28177:SF1">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 19, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10315">
        <property type="entry name" value="Aim19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ88463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="153" mass="16741" checksum="EA2970979C834E6D" modified="2017-01-18" version="1">MSSPPPVPAVPNVQSNKSLFKALYETSKTPYPAILASSTYLAQSLFAEPQPIKQVQSTKLWSSSSVTKFVMANPSKINNFLFGSVMGAASFMIIDGDLENGSGLAGIWSALYLIVNRKYLAMGLKHLRVQPVFANSLALFNAVIYGRRYLFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RTR1</accession>
    <name>A0A1E5RTR1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flocculation protein FLO1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g568</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000004">
        <property type="protein sequence ID" value="OEJ90244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RTR1"/>
    <dbReference type="InParanoid" id="A0A1E5RTR1"/>
    <dbReference type="OrthoDB" id="4070743at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000128">
        <property type="term" value="P:flocculation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001389">
        <property type="entry name" value="Flocculin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00624">
        <property type="entry name" value="Flocculin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1184"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1305"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1427"/>
            <end position="1520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1184"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1195"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1305"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1329"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1340"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1359"/>
            <end position="1389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1393"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1427"/>
            <end position="1461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1475"/>
            <end position="1484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1485"/>
            <end position="1514"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1541" mass="162494" checksum="62093E26A13DAD2A" modified="2017-01-18" version="1" fragment="single">TSDSVYSTSVFTVTNGSSPFISTVYFVATPEAHATFTQTTYWTGLNTSVYSTSYSTFAGVDGHETVNTVYYVETPVSRSSSTQFSYWSEATSSTYSTAVATVTISGSPVISTVYYVVTPEGHGTIERTISWTGLETQTYSTSFSTFSGTDGHETVDTFYFVETPISRVVETSYSYWSGAANSTFSTEVTTNYVFNSSALISTVYYVATPVDTSFSSFTKYWSGLFNSTYVATTLTSTDSAGNVFAEEVFEVDVSMPRSVITSVGFISESVASTYSTLYTTYTGADDVPTLLTVYYVNIPELFTDFTSTSYYHENVTSTYSTNIFTISSNESIIASTVYYVKVPFGSTSNTIMTSYNGSIASTYETSFYTFVGSDQKETIETVYYVETPKTSIEETATLFTTGNGSMIATYATSVTTFVGSDGISTTETFIYVSVPETQNTTSHSYPMSISTLSLSSSSYSSAASSASTDLKSLNTSVPASSFSTSVYSLSTMNISSTSLWSQSSSSLSSFFNSTSSTVSLSSSNHSKASTSFSTSEVSSYSSVQSMTVFNSSSSTTSLKSSTLQNISLSKTWSTTVSNATTSAISLSTSSTNGNSTVSLTGLPTVVSTQYVTFVGATSSIFETVTVTTTGVDGSPTVDVIYEIAVPDSSSTSVLSGVSDLQVIDSSSGSSDSFNTNDSYSVVVKLLDMSAGNSAWFTVPDEFTNLPSSVTSGSVTAILQSGSNNYTVTATGDSLQKRSTEETDLVLKLDLEESFRDTITESVTKYFTFDTSQGSFMVAIDFMDLTTTDPTSDSSISDNGRTLSWAYNIPLSVFTGNEIIFESLATTSYSLVPDSIELYWYSQTLLGSTSALITDGIENESTSELLLVVLDATKNTNYDFIRIEFQATTYDETEESYENTMNVYFDAGASSKKKRYSTYSITSGISKNGITPAPVVSVSENNLPGLPSVSEIEESAQSSLAALESFLSSSLASQTQVSSTIVVSSLSTTTTELPTPTIYSTIYGYYSGTTNTTVSSTLITTTGLDNKTTIEQVYYVDRPQDTSVIMESSYWTGLTTKTETSWATITGPDSQETIVTYYSIVAPISRGVSTVTVFYTGTTEMTYYTNTITETGLDGVPTMSTVYYVDVPMSTASSSSAPSAFTTIETMSGYTRTEIVSYYTTTPSGETPYVVATTYSIYANNSGPATSREIASNSQTTTRSETRTATGFTSLPISSSSFAGYFNTSSTTTPSEIQSSFSNGGTSSFMSSAESSSSMLSLTLSSITKSTTDNSIVSPTITSNAETPIISSETGSSFVVSASSDGNPVFSGTSSPVGGATSFESSPVETIPQSAPTSTSSATSVIGSIESQRSAEPTPGPVSGSETDFTGTASVSDTFTGTQSMNTVINANPGTGSGSYTNSSAPTSSFSSGDGGSTRIGSSVQYSATASVGSASSNSSSSFPKTATTTTNNNNEVGGSLNNNSSTRSTERTVLAPEATSGTSDTRGQPQATTALSSSPSAVSPLSGAAASSATAQVPEEYTGKGSRTSFKLYTVVLSMLVVLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVB8</accession>
    <name>A0A1E5RVB8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Aspartic proteinase yapsin-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g332</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVB8">
        <property type="interactions" value="365"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVB8"/>
    <dbReference type="InParanoid" id="A0A1E5RVB8"/>
    <dbReference type="OrthoDB" id="3972413at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05474">
        <property type="entry name" value="SAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000011">
        <property type="entry name" value="Aspartic protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000023">
        <property type="entry name" value="Aspartic protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033876">
        <property type="entry name" value="SAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF65">
        <property type="entry name" value="ASPARTIC-TYPE ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184889" evidence="9">
        <location>
            <begin position="30"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="10">
        <location>
            <begin position="86"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="464"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="531"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="613"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="664"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ90871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="713" mass="74874" checksum="CB6CE9386D69B64F" modified="2017-01-18" version="1">MNXHQNSSFGAKLLQLFRSFLPLVYLVTALENEDDXKSLAFSFDKYVGDGDFEKSKRNARPYIVSTDLNKRDGDATFSLTNKKTFFSVFLDIGTPSQSLTVLLDTGSSDLWVIQNCGSSSCPQYGTFQPAKSSSFYNNQTEFYVTYGDNSYGSGSWGYDKVSINGQSVGKLSFGVATQSSSKIAVLGVGMTGLETTYSGKKVSSPYEYANLPMALKESGQIHTALFSLFLNDLTASTGSILFGAVDTSKFSGNLYTLPMVNIYAGSGFDNPVEYEVTLQGLGLSSSSSETTITTTKFAALLDSGTTLTILPTSLMDLVVDALKASYDSSVGYYEIECDSTSDQNMVFDFGGFHISVPLSDFVIDSSSGKCYLGFQGASQSFVILGDVFLSNAYAVFDIDNYEISLGQANYNGGSNVQEVSXGSAVPNAVKASGYYNTWTGYQDAVSGGNIFTLDSSTGIEKSSTVSXSTSFSSPSTATSSTTPSSPTVSSTRSTTPSSSTTSSTSTTFIPSSSSSATVSSTLLESTSIISPSFETPTSSTIPEITTTTEQDSTSKSVTTVSSLLGSTLTSSSSSSSSSFSSFSIESSVTLSSSSSNPLSTTILSANSSNTAHIQTSTAHSSTHSSATLSKDSSMXKSGSLSTKKSTSTSAVSSKTFEKPLTTSLSSASSTXSSAATDANSNSSTKKSKNAGNNLELPRWIVSSILIYLVQLFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVE1</accession>
    <name>A0A1E5RVE1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TBP-associated factor 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Transcription initiation factor TFIID subunit 12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g329</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVE1">
        <property type="interactions" value="538"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVE1"/>
    <dbReference type="InParanoid" id="A0A1E5RVE1"/>
    <dbReference type="OrthoDB" id="3972627at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07981">
        <property type="entry name" value="HFD_TAF12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000011">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037794">
        <property type="entry name" value="TAF12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003228">
        <property type="entry name" value="TFIID_TAF12_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12264">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12264:SF21">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03847">
        <property type="entry name" value="TFIID_20kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 12" evidence="9">
        <location>
            <begin position="499"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="63"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="356"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="601"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="73"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="147"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="356"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="393"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="412"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="604"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00075089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ90854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="639" mass="71143" checksum="87F3D46A913F31EC" modified="2017-01-18" version="1">MSQPPNRLPENVSDEVLRQIGEKSQNYIQEAQKVGVSTDQGRDFLKKGQRLKEVYMMYVKKRQAKAEQEKANNGANEGGSTQSQVNSQKVPPTSDHQTSNVATNXQPAEVPLSGTEQDSQKTKEQQKESTLPQQPQQNVELPPQPVQKDSNAQSQKTHPQEARNDAQPTGSQASPPPQGSAAPNSITIRQLLDEEQTKKYNEMQAKNSDHAKRIVEQHTQCRRQIEALDKEVKNDPSDPKVPELQASREQLVAKLRNIAIAFKRLEQMISEEKRRFYLECAQVNQPLRRFLQARQLQAQAAQHQQQQQQQQQQQQQQQQTPQQSALQPQQQQQQQQQQQQQQQQVQQQQQQQQQQQVQQQSAPQHSTTPSAPQPVPVSVPPAEQAGSTPVVGSENQKTSKQNVTSNTNQNGAPGARATSVSAAAAAANDQNVKPQAIFKQSPPNIPLSDHVQAKELEPVPYNSGRPTVTGGAANGMASINTPAIIKIPPYEVEGDRVMSKRKLRELVKTVGVDEGDGSTTVDGDVEELLLDLADDFITNVVSFACRLAKHRKSENLEAKDLQLHLERNWNIRVPGFSADEIRPTSKWNPAPQYVAKMAGIKQQAKLEQEQKEQEMEQNAAAGLEKDDQSVEPATKKPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVK8</accession>
    <name>A0A1E5RVK8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Thiol-specific monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g375</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FMO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVK8"/>
    <dbReference type="InParanoid" id="A0A1E5RVK8"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038732">
        <property type="entry name" value="HpyO/CreE_NAD-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13454">
        <property type="entry name" value="NAD_binding_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00370">
        <property type="entry name" value="FMOXYGENASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-dependent urate hydroxylase HpyO/Asp monooxygenase CreE-like FAD/NAD(P)-binding" evidence="6">
        <location>
            <begin position="7"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ90982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="512" mass="57778" checksum="188D87BDDF565915" modified="2017-01-18" version="1">MTIKSIAVIGAGPTGAGLVKALIKERHFAKIKVFEKRNSFGGLWNYTKPQINKSHISDAAKTYLEKVPLDNPFHRISPIACQQMGSQDSTKKSLVFDSAVYRDLDTNVPKTLMEYNKFPFPADAPLFPTRDTVLKYLSDFADPIKKHVQFNTEIVDLQYDPQSAKYKIGYIDHAPAPQKTKQESSTVYDPEEYDAVVIATGIYDLPYVPSKPGLEKWFKTYPESVLHSKNYDSVDQFKDVKGEIVIVGNSASGNDLAYQLASKFKRKIYKSKRSESALPGGKSSFIVELGDISEMNSANKSISFTDGTTVENVEKVIFCTGFLKSFPFLPTSQNNKQTSPLSNLITDGKMVHNLYNHILPINLPTLAFIGLPKYSLPTRLSETQGAWLARVWSGKIALPSKELQWKYHHWFKAKNETESKYHEIGFPMDVQYSQRLNREIRHAGNANGYFGIEWTGDQIKXRSSIKPLKEGYIKYFEETGQRASSVQELIEQGYFEWPEDAVSCVQVPSFAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZF5</accession>
    <name>A0A1E5RZF5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative bolA-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BolA/IbaG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZF5">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZF5"/>
    <dbReference type="InParanoid" id="A0A1E5RZF5"/>
    <dbReference type="OrthoDB" id="411584at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044572">
        <property type="term" value="P:[4Fe-4S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.90">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46230:SF7">
        <property type="entry name" value="BOLA-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="137" mass="15473" checksum="783ADBABA430AC03" modified="2017-01-18" version="1">MLKAPLRXHKLLHTQIACMSFQTGLAPNRCTDGPICQQIIAKLQKGDSSSTISSANITKLEIHNDSYKHVAHAEQKNLPNRIESHIRLLIVSDGFKRMPVVKRHKLVYSLLAEELARDVHALQLTLKTEEEYKKLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZJ6</accession>
    <name>A0A1E5RZJ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">intramembrane prenyl-peptidase Rce1</fullName>
            <ecNumber evidence="10">3.4.26.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g274</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Hydrolyzes the peptide bond -P2-(S-farnesyl or geranylgeranyl)C-P1'-P2'-P3'-COOH where P1' and P2' are amino acids with aliphatic sidechains and P3' is any C-terminal residue.</text>
            <dbReference type="EC" id="3.4.26.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase U48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.26.1" evidence="10"/>
    <dbReference type="EMBL" id="LPNM01000002">
        <property type="protein sequence ID" value="OEJ92143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZJ6">
        <property type="interactions" value="293"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZJ6"/>
    <dbReference type="InParanoid" id="A0A1E5RZJ6"/>
    <dbReference type="OrthoDB" id="3971446at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039731">
        <property type="entry name" value="Rce1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13046:SF0">
        <property type="entry name" value="CAAX PRENYL PROTEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13046">
        <property type="entry name" value="PROTEASE U48 CAAX PRENYL PROTEASE RCE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 14" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="89"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="350"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="13">
        <location>
            <begin position="206"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="45"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="45"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ92143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="372" mass="43269" checksum="7EF2FB4B2DF23DF3" modified="2017-01-18" version="1">MSSLIDQYSVTKSIFVSVLYVAAIYTSNVYLKHFHFKKSVKSTRKKSDNCKNKKQDEKSELEVDSDSEEEQNEDNKLKRDNPIVIRSRMKFVSFAIAIILLTMYSTHGDWKQLIGAQYYVLKSQSQSVVVTTTWTKYLGQVFQLVGXYLILYSSQIVDTFLFNKIVIHAGASSENSTPNTYTSSSFSFFSLKFFFQLFEKLDVWDFRNLIFAPFTEELVFTAIILYNYKLQNSWWLSLYFGIPHLHHAWELIQEMDSNPNYTIGSIFANCFLQFCYTSLFGHINNVIYLKTKQNLACCFVSHSICNYMGFPNLVPLFSIEPGVTTNSSANHSAATIDQQKKVQKQQLNKLYKYMYWSLMIFGLIQYIKYLTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZM3</accession>
    <name>A0A1E5RZM3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WKF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g81</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZM3"/>
    <dbReference type="FunCoup" id="A0A1E5RZM3">
        <property type="interactions" value="223"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZM3"/>
    <dbReference type="InParanoid" id="A0A1E5RZM3"/>
    <dbReference type="OrthoDB" id="10261563at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019327">
        <property type="entry name" value="WKF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22306">
        <property type="entry name" value="CHROMOSOME 7 OPEN READING FRAME 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22306:SF2">
        <property type="entry name" value="CHROMOSOME 7 OPEN READING FRAME 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10180">
        <property type="entry name" value="WKF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WKF" evidence="2">
        <location>
            <begin position="117"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ92401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="303" mass="34569" checksum="B160D108FC146AF9" modified="2017-01-18" version="1">MSAHVPAWKRIALKKQEADKASASNISSSNSSSNSNHNNLDNDDPFNVTTHLATGSLTKKEKRKILKGEDLATNKKVKKAKHKSGSDDASTNKKKEKLSKEEKAAHLQTVLKDQFRYLIEFYVFKINADIDTLPKEILELPSVSKNMSTLKPDKDSPILKLWKFNKSKQNWLLKNMFHIDDEIPEQYDYLLVQYFKSLKGQARDQLVEECLKILNSWNEYVHEQQLEMQKILEGGGKEETQQLEPKNTDVTGGKKVEEGASKNTEEAFKDTKVPPSKKVVKRALALLEELDSAKIIKLENWED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZW0</accession>
    <name>A0A1E5RZW0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative proteasome subunit alpha type-7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g229</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZW0"/>
    <dbReference type="FunCoup" id="A0A1E5RZW0">
        <property type="interactions" value="1269"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZW0"/>
    <dbReference type="InParanoid" id="A0A1E5RZW0"/>
    <dbReference type="OrthoDB" id="40134at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03751">
        <property type="entry name" value="proteasome_alpha_type_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000044">
        <property type="entry name" value="Probable proteasome subunit alpha type-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="5">
        <location>
            <begin position="8"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="248"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="255"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ92376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="287" mass="31409" checksum="9F892D7584ACF841" modified="2017-01-18" version="1">MTSIGTGYDLSNSVFSPDGRNFQVEYAVKAVENGATSVGLKCQDGVVFGVEKLITSKLLVPGKNRKIQTIDRHIGCVYSGLLPDGRHLVNRGREESANFKKMYGRPIPVDAMADRLGQYVQAYTLYNSVRPFGITAIFGGVSKDDGKPELFMLEPSGTCWGYKGAATGKGRQTAKAELEKILTVEAEQDDEVLLSAKDAVKQVAKIVYMAHEDNKDKDFELELSWCSLSETDGVHKFVPKELFDEAVEFAKKEMEGEDDSDEDSDDEPAAGSGSNDQDAEGDIKLEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZY1</accession>
    <name>A0A1E5RZY1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Putative di-and tripeptidase DUG2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g54</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZY1">
        <property type="interactions" value="93"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZY1"/>
    <dbReference type="InParanoid" id="A0A1E5RZY1"/>
    <dbReference type="OrthoDB" id="7832001at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051458">
        <property type="entry name" value="Cyt/Met_Dipeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017149">
        <property type="entry name" value="GSH_degradosome_Dug2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270">
        <property type="entry name" value="BETA-ALA-HIS DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270:SF8">
        <property type="entry name" value="DI- AND TRIPEPTIDASE DUG2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037237">
        <property type="entry name" value="Peptidase_WD_repeats_DUG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="63"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="9">
        <location>
            <begin position="715"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ92444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="961" mass="106960" checksum="67C05D8757C5093B" modified="2017-01-18" version="1">MSDTPPLLHRWEHKYSILSTVAFPHKKILFAGTQDSKILCFDLTTYSLIKTINVQTQVSLNEQDNTKASVLCLSKSKNEKFLFAGGSDSLIRIWKIHVIENMGNKNLYLDKTKDIAIEYVEESETKKDNAESTNTEYFVPGKSVTTIIEATEIATIYSTLDIGDIFSLIYLDESQTIIFGTQRAALLYVSDIFNAARSSKRYELLPHNRYDKFFDSMGPAGHLLPRQPRSSFSSISSAENQTIPQLRSESPGPGSRLSSPDSYVATGQEHFIIEVPSTNIISYAHNGFIYSLFKYENSLMENNQEHVISGAGDGVSKIWKLNPETLELEFQFQLPVSNSAAQSGDLDEDSSDDECEDDIIFSQTVQFPFLYCGMNEGNVKIWDLNTKQLISKINCGGSLEDVTSICCWEGCVFAATKNKIVTFKENKVICWVAHKDTILSMSFLRPKCTKCKNIRLISGSADGSLVLWNTDSLLGELLPESHSAGERRVSHSHAHLKDSNLPPNLHTALELTNDHLIDQLKNLIKFQTVSSLTNDTHAILDSHSCAAYLIKLFSKFGAAKTKLIPVASGNKAPIVYAKFYANSLDEKFNAETQTFEAHGKPKNILWYGHYDVVEPGNDWNTDPFTLIAENGYLKGRGVSDNKGPILAALFAVADLFSKQLLKNNIVFLIEGQEESGSNGFINSLQEANINLNQKIDSILLSNSYWLDECTPCMNYGLRGVINMRVTITSDEPDRHSGVDGGVYQEPTTDLFQIMSKLIEGPKKVKIPNFYTPLKDISSKEVSHXKDIVERTKINTTVENLVAKWTKPSLSITTMNGGVNTTVIAKTANATASIRIVPEQKLQQVKKDTIEYLNSCFEALESSNHLNISILNEAEPWLGNTESKAYQVLKNEIQEVWGVEPLLVREGGSIPTVGLLEKYFQAPAVQIPAGQSTDNAHLGNEQLRILNLLNLRKVVTKTVNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0K9</accession>
    <name>A0A1E5R0K9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R0K9"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184545" evidence="1">
        <location>
            <begin position="42"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="334" mass="37408" checksum="E61E115F65E905F6" modified="2017-01-18" version="1">MPSKTNQSTRTAISNINKHQRSMSLLKIFFLLGCLLPLSNAAPFRKGKTITVTKTVTLQPIHDYNSMPTAVPELPLNKDKPAYEEDEYATDNYEITEDWDKEYTPTSTWDDTYASASTWTEEYTPTSTWYDTYASASTWTEDYTFPYTPVETDAEEVWDNPLLTRNAVPQWVTIDEDWEYPATKIHEQKEETHVLGTMTETFTKTKMQPLSKTQTTSKPSFISKTRKSAYETSSPISTVSTCTKSKECGATQMASVMKSYSPVMNNATVVTSFVKFNETAVSTLTFHPPMXGNSTXGNHTIPAFSTGAGNALHGVYNTVGQFVXLLGVALFYXF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0M0</accession>
    <name>A0A1E5R0M0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Rhomboid protein 1, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g4628</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0M0">
        <property type="interactions" value="638"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0M0"/>
    <dbReference type="InParanoid" id="A0A1E5R0M0"/>
    <dbReference type="OrthoDB" id="10260614at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000012">
        <property type="entry name" value="Rhomboid family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050925">
        <property type="entry name" value="Rhomboid_protease_S54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731:SF14">
        <property type="entry name" value="PRESENILIN-ASSOCIATED RHOMBOID-LIKE PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731">
        <property type="entry name" value="RHOMBOID PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="301"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="330"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="365"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="8">
        <location>
            <begin position="238"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="404" mass="46084" checksum="7A8D7F524F35A896" modified="2017-01-18" version="1">MFPTRARLFSVKGVCLSKSTIERGHDFTRSHGKLFFLSNAVHLQNKQPLRLGKPLMSSMMGMPILKVMQRFGLHGKSYFKQTFHQYGNAPSQGTQSGFFFAQYLKLPGTSLNRVTLFKRFLSPLAFLAKRSVFNGSNFSRLNRFGNPSSYSRNGNHNNNNSIKNMTIFGLIFMGXTFFATPYLFQYVPVFTYFQKNPQAMVWSIIGINALVFALWQFPRNWYFLRRYFMLEKDAIFSKWSLIGSAFSHQEIWHIGANMLCLWSFGFGLANMLGSAGFFSLYMNSLMLSSLFSLWYPRIMKLMIRGPSLGASGALFGVFGAFAYMFPHAKIMLFVFPIPGGAWVAFLGGALWNLAGCVFRWGTFDYAAHLGGSLMGLAYGYYFQEIIRKKRAQQSRKITSLRDLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0V6</accession>
    <name>A0A1E5R0V6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Guanine nucleotide exchange factor subunit RIC1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4418</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0V6"/>
    <dbReference type="FunCoup" id="A0A1E5R0V6">
        <property type="interactions" value="159"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0V6"/>
    <dbReference type="InParanoid" id="A0A1E5R0V6"/>
    <dbReference type="OrthoDB" id="67540at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034066">
        <property type="term" value="C:Ric1-Rgp1 guanyl-nucleotide exchange factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040096">
        <property type="entry name" value="Ric1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009771">
        <property type="entry name" value="RIC1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22746:SF10">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR SUBUNIT RIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22746">
        <property type="entry name" value="RAB6A-GEF COMPLEX PARTNER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07064">
        <property type="entry name" value="RIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RIC1 C-terminal alpha solenoid region" evidence="4">
        <location>
            <begin position="938"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="15"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ80538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1087" mass="125925" checksum="9038C2F35D0D069B" modified="2017-01-18" version="1">MHIWPIFAPQTFELPQTSSKTNTAKPRDNSDEREETTEENFLPHQGHQQEYTNGEAILETITITDTNLIIFLTESRILLYNAKPIAPVSVYQRSHESLLKFGTNNFMRYNNPYHRMNGLNDKVEISVYRNKIIVYVVTESSFLLVFHVSTTVNSFDVYKDYGIEPNLYNEKFSSAEAWINQILNEHNDFESNSLAAAVGDSIDTEVLTVFDQNNGSRVIQNGYIINKEKNFLQLMKSLMQMKEDFSEEMPIRMGDILLKKVLKFDHKIVDLFGLQGSFINNNKFNRQEQLCMLFENELHFLTLENYTLVKNEVIKLANSLRLVFNGNDLFVINQNNNVESMGSLSINVIKKLEKTVYTKEIKENLPTESLLNVFNFGERYMILLFETELVYFDTVKNEVYQIVKLHLNVLLKDQEDKVVTGGFLTNDLMLLVTKKGEFQLYSKWGNLLAQINLFKLSEVEHEEYTGFSFIDNLIVLTTKNGVFQIMQCWKEIFNKSFCNFRTLSQYVLFNSQTNDLLIYQPTNCKNSVKPARIIKLPTKFFNNSIGKVEINGDLSIMATYVSNKKVLLMTDLQKFSNWEIFSSLEIMDMSWLGSNYLICYMNDELENGGGKIIRCHDFNMVLQQSTTEKSNVRSLSDGKVWTYRVNRDDTLLYWNSNTLFKYTVVKKKSTNEVLYKTGEINILLQGADNNVRLETIDILSKTHYSKENQIQSFVKHTIDLSDMSMFKFYHIKWIYRYSLKGVLIYTNDDEIYKISEDHSSSGTETPCFKSIKLLSKVERIVDILENNIVLIKDQRLLIFDLQDFESFTNTTIEEDEDAKIDPIVSNVISEEEYPIMISKNFSVFQSLQCFYQKGTNFQLQLHDGIILNQIVDSYITLRKIDPLVVTEKFYTSKYYMFALEKILSSRVVERRNFDDIMQLIDHYDIKCFEEGNKRNEKRLCIISNCLRKIEANRWSYLFDNSDISPKKLLSDCIQLNNGKLLGALLMVFLSYEDVESQSNTPTPAPAILTEMKASDGTPSMGNQRDSIINGALVKQVLDVLIENAADSTDASAAQEHWSMAFQLIRFLKIYDKENLSDLTKQALESIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R167</accession>
    <name>A0A1E5R167_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA mismatch repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g4303</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the post-replicative DNA mismatch repair system (MMR).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutS family. MSH3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R167">
        <property type="interactions" value="828"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R167"/>
    <dbReference type="InParanoid" id="A0A1E5R167"/>
    <dbReference type="OrthoDB" id="121051at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF122">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="9">
        <location>
            <begin position="942"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="44"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR037677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1101" mass="126005" checksum="DAB9067E0E18C0F2" modified="2017-01-18" version="1">MRPATIQAESTIKEGDHAADGTFTSVLPLNNTDHAVDVAQNESIVLNSSDEEDTTPSTTFSASETDDXQRLDPAKTLPNSEPEKRNLDHVKQDNSSGLLKMQNFAFVKSSSNDPSFSKKTSDIDXXRSQFNDKLFXIMDSKGKRKLSXDENNEELDLSDNPEIESTTQAISRKNKKQKKATDLTPLEQQIXEIKLXNMDKIIAVQVGYKYKFFAQDAVIVSQILHNMLLPGKLTIDESNPQDKHYKKFAYCSIPDNRLHIHLQKLLHYNLKVGVVEQVETQAIRDAGQGSKLKNVFERKLTNVFTKATYNMNENFGKANGGSCSLKNASAICAIVTERQTXEANSSSSTSNVFKIVSIELDTGLVTYDAFXDSTKFLSKLETRMKHLNPSEIVHNNXLDKKIQTYISKLSENITFYAFDDNEIENLTQQVNSASVXDIIVNEHLEKPFNLLYMHLRTFNLQNSLLSLSPKTFQAFESTQFMNLQANVLTNLEIFENNTEKDTLKGTLLWVLDHTRTSYGFAKLQHWISHPLVHKDKINARLDAIECISQEVFNIFFESLNNWLNDSQNKNLLKTVNRIKYGKTSKKEVYYYLKSINFLRELFKKHGKYLTEQVYDSIHGRVALKSQLLTEILMFCNETCSNLQVEKYLTMINVESVFHKSNQQDDEHFNFFNLNNYDDSQXILAKQRDVEGVKHDLQDELKNIRKILRKPMCEFKDEVDYLVEVRNSLVGKIVPPDWVKVNSTKMVSRFKTPNVAILLKKLQLEKDLLHQVTENEYFKFLQRINDEYDQVFAILDNLAVYDCILSLSSASYSNDNYCRPXFTSTKTQYINIVDGRNPIIENMMLSSDSYVSNDVHMGVGNNQIMVVTGPNMGGKSSYIKQVALLVIMAQIGCFVPCSFMEISIFKSIFVRMGAQDSLIQGKSTFQMEILDILQILNNFNRDSLLLLDEVGRGTGTTDGMSISYSILKYFIDLKNDCPLVMFITHFHSIIKKFRNDMLGIYHMSFYQDQNNDDTDITFLYKLTKGYMNKSFGMNVAKLASIDENIIXQARSLSENLQLESDSNKDIYTCKKIINLLLWMSTAENSEGWXSHVSEFQQYIDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1C8</accession>
    <name>A0A1E5R1C8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">FAS1 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4038</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1C8">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R1C8"/>
    <dbReference type="OrthoDB" id="286301at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050904">
        <property type="entry name" value="Adhesion/Biosynth-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10900:SF77">
        <property type="entry name" value="FI19380P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10900">
        <property type="entry name" value="PERIOSTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184560" evidence="3">
        <location>
            <begin position="22"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="911"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="4">
        <location>
            <begin position="80"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="953"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="962"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="985"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="998"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1027"/>
            <end position="1037"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ80708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1043" mass="118167" checksum="5A6C86BA1DD87C34" modified="2017-01-18" version="1">MRFLFCFIVLGAHFILQSTGSLSSFEFSPKPYTGAAATSVDADEHNFLPSSAISITANPTDQTFQNLHSTAAEDDPMDQYTTVVDLLSENVIFSKFLQFLQHNDYIDCLNGLDNFTLLAPINSAFYIPNENESEEELAWKRQNFRLENYLIKDNIVNISTDFHNKTAEQKGLFVLSQGTDYPLVWEKKNANTIILYQHPAITHDGQDNKHSAAIINATVHDAQSLRILTDSIYKPTLQNCVVYAVDHLLPLKFQNLMNFFESSEQDLHIFGNFTQKTDYLPWREYLVNNTIVTPTDQLLQDNFNDVELSYLLTNCDAVSRDFAVEMDSNDVALSLRKILKKRDPYSDLQQDQTIFAKNFVFEGFLGGLQETASKSTRNLNKVTFAVDTINHGKCLKINDSVSETKPFVVDYLTQNDEPGDFGMGYVFGNFGLSSIHDSLQFNALKYLLGLQAFDFVDELIFRNLDYLISNNEQELTLFVSVSESDFASAEQVFERNDPVDQYSAGYSSSILIYHFIEKKLDLQTFEKSVKTNPSYKNHISWQSYMVDSMYCEKNKLMGRSCQKLKIWKKSYEDEHEYYVNSPSTKILNKTPFVVGNTLIYVISRQLALPATIAQSVGSFLHCSTSLLFLEQLNLLELPPNNEGYTILLPCYDSWENLQLNFDVIQKNVTALNTIMKNLIWNGLFYTDTEENLAFVTSTLNGESVNVSRVKSDDADKIHLNVSSLRNSLVEVDKNNDIMFNQGVIHPVSSAYYPDSLKISLRDLILVSESIDFLMLTKDFEHLSAILENDLPYSILVPTPAALFYDGDIHANATRLEDILKLHFIAENSTEALFQCSEDIYTEYGEPLSCRKVDSDNYFLRLKNGADKEVRILKKGCSTNNPHSCVFMIDKPISLSWLTKKRYILHLPYESFAIGILIGVIGFTALSLLVMYAMSLRGRKAAIEFDDATEDALSERSSQGSTRAHGXSSRGSQRRLSSSSLLQKHSSARRPVSKHSRSDYQSIQDSHVSPGIQPSNSHNIETPGAFESQYSENSQSRPITMRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1H6</accession>
    <name>A0A1E5R1H6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Vacuolar amino acid transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g4036</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1H6">
        <property type="interactions" value="339"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1H6"/>
    <dbReference type="InParanoid" id="A0A1E5R1H6"/>
    <dbReference type="OrthoDB" id="655540at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF692">
        <property type="entry name" value="TRANSMEMBRANE AMINO ACID TRANSPORTER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="320"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="347"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="379"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="421"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="462"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="497"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="596"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="619"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="654"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="10">
        <location>
            <begin position="241"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ80727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="677" mass="73737" checksum="7E018D235DC042D0" modified="2017-01-18" version="1">MSDHDLEHGKNAKNNTQSPSTPQYFSIPITNTQDNHGRSPASPSGHFHYEASSPGEEQESNARNHYGSIDIGQPSHRRPSIFEQPIGSFRGPNSLSRFASSFQRSNAFKTIDLHHDKERSYFKDGTDETFDPETLAPSMHGRRLSLLLNNSAINSPNGQLSGGFASDSLPSRSMANKPITVNDVLMHQPVFDDRDSTLSVAVSNNNLSAFPSNSRNQDVQSVILKKFETEQGTTVVTLAGQSTAPQTIFNSINVLIGIGLLALPLGLNYAGWYLGIPMLLIFAFSTFCTAELLSRCLSTDPTLISYGDLGYAAFGPRGRALISFLFTMDVLGAGVALFILFGDSLNALFPSHSVTFYKICGFFIICPQSFLPLHILSHVSLLGILSTTGTVFLIFVCGLSKASSPGSLLEPMVTNVWPANFTNFCLSIGLLSACWGGHAIFPNLRSDMRHPEKFKDCLKTTYLITSITDIGTAVLGFLMFGNGVLDEVTKSVLLTKGYPSFVYVLISALMSMIPIAKTPLNAMPIVSILDTVMGLRSNNHKDKEKEMLANADSAGNXARDIRSNDSSQDWLLSSSTSRPASVENRLLKIKQKVGGVFNRILVNVLFLLTSILYPQFDKLLAFIGAGLCFLICFILPCSFYLVICKNTIKTWEIIACYIVIFVSIILSVLGVGAAIVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1S7</accession>
    <name>A0A1E5R1S7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Serine/threonine-protein kinase YPK3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g4483</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1S7"/>
    <dbReference type="FunCoup" id="A0A1E5R1S7">
        <property type="interactions" value="148"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1S7"/>
    <dbReference type="InParanoid" id="A0A1E5R1S7"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000913">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="255"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="9">
        <location>
            <begin position="553"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="637" mass="71272" checksum="C51CD6C0FD6B7205" modified="2017-01-18" version="1">MVFSLDEEFPISEMQDPPPDLISNGTCIMKPALTSCKDNHEVLSFSDEDDDIGRNEKENQPYHKNLMQPNPRATPLHNAAAKLSIPVPTKASLSFNTAEAEGINSFCQEANINTARSKLQTISSHDNLTGKHSISVKNSFLKRNAETPILSGTLAVPIDSQRPEGRNPRRRSSVLSRISINTPPNAVRVGISASNADCSVFDDEEKSDEDFVDSCGMEFLSLNNSKPVVGNTTETVAIGGSVVSKHIVKRKIQDFQPVRVLGQGAYGKVVLVKDQYNAKLYAMKQLKKAEILVLPDDENEKSQGGMSPRSKRIERTFAERTILSTLEHPNIVKLFYSFHDTDKLYLLLQYLPGGELFFHLKNLGNLEEDTVAFYTAEISLAMKFLHEKGIVYRDLKPENCLLNERGHLVLTDFGLSKSSHGGDGNFYSIIGTPEYAAPELLQGVAYTKSCDWYSLGCLVYDMLTGKPPYTGQNPKVILNKIMKNTLKVPYYLSEGMKDFVNAILKKDVEKRWNVDLYWDESLNKNRGKPADKKKKKKAGAAKTTGFKQHFIFRKINWKDLETSSFQKSTFGPIMPVITDWSLAENFDLEFTEQKLQSGDIRSVDMSSPAGPANSAMHDELFKGFSFTASSSYLDRYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1T6</accession>
    <name>A0A1E5R1T6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Importin alpha re-exporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g4366</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the XPO2/CSE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1T6">
        <property type="interactions" value="1201"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1T6"/>
    <dbReference type="InParanoid" id="A0A1E5R1T6"/>
    <dbReference type="OrthoDB" id="3268246at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005043">
        <property type="entry name" value="XPO2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013713">
        <property type="entry name" value="XPO2_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF8">
        <property type="entry name" value="EXPORTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03378">
        <property type="entry name" value="CAS_CSE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08506">
        <property type="entry name" value="Cse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="8">
        <location>
            <begin position="23"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="955" mass="108755" checksum="57E98419D9A99F87" modified="2017-01-18" version="1">MSDLDVISNLLTQSVSAATAKSAEKQLKEIENQQGFSLTLLNIVQSTNLPLGTRLAAALFFKNFIKRKWVDEDGNYLNNXSDAIKAQVVDIMISLPNNLQSQIGESISLIADSDFPMRWPTLVPNLVSKLSNDDMVTNKGVLTVAHSIFKRWRALFRSDDLFLEIQMVLDLFSVPFLQLFQSVDEQITANSNNQAKLVLLFDVLLVLVKLYYDLNCQDIPEFFEDNMKKGMGIAHKYLGYHNSLVEDETDDDHASIVLMVKSSIQELVQLYTTRYEDVFSPMINDFIQTTWELLTNLAPKAKNXVLVCKSMEFLSSVSRIPAYFEHFNSETSMNTLITQIILPNLTLHDSDEELFEDDPIEYIRRDLEGSDLDTRRRACTDLLKVLKEKNKQLVTTISLQHIESFLAKYNGNQAENWKYKDLAIYFFLAIAVEGNVTHHGVSNVNEYVDVMQFFRSDILQHLNGSNTQNPIINVDAIKYVYTFRNQLNKEQLIEILPYLASFLQNANYVVYTYAAITIERILSMRVSPTSSKFIFQKEDLAGSAELLLTNLFQLILKDASSPEKLSENEFLMKSIYKILSIAEDVIQNSALRILSQLFEIVTITMKNPANPRFSHFTFEAIGVIIKFNCRXSILEVVEQMMPTMLQILAEDVQEFVPYAFQTISYSLEILPENVEVPQSISQLSVPLLTPSVWEYKGNIPAVTRFLKNLIKKNHQIYPDLVPVLGIFQRLISSKILDVHAFELLEYLVDYVPVERLEPFLKEIMVLILQRLQNSKTEKYVKRMIVFLGNVSIIKNSDFVVQLFDNVQDGLFQQIWNNIVLKTLPTIGNLLDRKIALVGLFNVILSSSLFSTKYSSLIVPSLDVFIDFTVSESVVNMRADDMDIESLEEITTFGSSYSKLISIADKPFDPIPQIDYIDGVKKAVSELLVKSNSLLISFHPQLSESARKGLIELGIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1V2</accession>
    <name>A0A1E5R1V2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Bifunctional purine biosynthetic protein ADE5,7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AWRI3579_g4363</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the second and fifth step in the 'de novo' purine biosynthesis pathway; contains phosphoribosylamine--glycine ligase (GARS) and phosphoribosylformylglycinamidine cyclo-ligase (AIRS) activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ATP = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>5-phospho-beta-D-ribosylamine + glycine + ATP = N(1)-(5-phospho-beta-D-ribosyl)glycinamide + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17453"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">In the C-terminal section; belongs to the AIR synthase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the GARS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1V2"/>
    <dbReference type="FunCoup" id="A0A1E5R1V2">
        <property type="interactions" value="1218"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1V2"/>
    <dbReference type="InParanoid" id="A0A1E5R1V2"/>
    <dbReference type="OrthoDB" id="2018833at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00125"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004641">
        <property type="term" value="F:phosphoribosylformylglycinamidine cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046084">
        <property type="term" value="P:adenine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02196">
        <property type="entry name" value="PurM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000006">
        <property type="entry name" value="Phosphoribosylamine--glycine ligase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000006">
        <property type="entry name" value="phosphoribosylamine--glycine ligase, chloroplastic-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.10:FF:000001">
        <property type="entry name" value="Phosphoribosylformylglycinamidine cyclo-ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000018">
        <property type="entry name" value="Trifunctional purine biosynthetic protein adenosine-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.600.10:FF:000001">
        <property type="entry name" value="Trifunctional purine biosynthetic protein adenosine-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.650.10:FF:000007">
        <property type="entry name" value="Trifunctional purine biosynthetic protein adenosine-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.10">
        <property type="entry name" value="Phosphoribosylglycinamide synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00741">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00138">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020561">
        <property type="entry name" value="PRibGlycinamid_synth_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000115">
        <property type="entry name" value="PRibGlycinamide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020560">
        <property type="entry name" value="PRibGlycinamide_synth_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037123">
        <property type="entry name" value="PRibGlycinamide_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020559">
        <property type="entry name" value="PRibGlycinamide_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020562">
        <property type="entry name" value="PRibGlycinamide_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004733">
        <property type="entry name" value="PurM_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00877">
        <property type="entry name" value="purD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00878">
        <property type="entry name" value="purM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520:SF12">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01071">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02843">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02844">
        <property type="entry name" value="GARS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01209">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01210">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00184">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="18">
        <location>
            <begin position="119"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OEJ80862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="800" mass="85790" checksum="885777C917743DE6" modified="2017-01-18" version="1">MVCKNNLNVLILGNGGREHALTWALIKSPSVAKIYVAPGNGGTSTINSNVVNVPELSPSPENFEQLSKFAIENNIDLVVPGPEQPLVDGISQVFKKIGVPVFGPSADAAIFEGSKTFSKDFMKKHDIPTARYENFENFEKASEYLTKNVDYNVVLKADGIAAGKGVLIPSSKQEALEGLKTIMVEKKFGSAGDQVVIEEFLEGDEISILTISDGYSYFNLPAAQDHKRIGENDTGLNTGGMGAYAPAPVATPKLLTQIDQQIIKPSIDGMRKDGMPFVGVLFTGIILTAQGPKVLEYNVRFGDPETQTVLPLLSDSTDLAQVFLAAAEGRLDSVQISIKPNTFATTVVLAAGGYPESYKKGDEIVIETEKINASNTYVFHAGTKLDNNNKLVTNGGRVIAVTSTGSTIKDSLEAVYAGVEHVKFNNRYFRKDIAHKAFRDASANETSITYAAAGVSVDNGNLLVEKIKNKVKSTRRPGTDSDIGGFGGLFDLKAAGYEVDETLLVAATDGVGTKLRIAQETNIHDTVGIDLVAMNVNDLVVQGAEPLLFLDYFATGALDIKVAADFVSGVADGCIMAGCALVGGETSEMPGMYAPGHYDCNGTAVGAVNKHSVLPKISNMQEGNVLLGLASDGVHSNGFSLVRKIIEHVGIQWEAQCPWDASKTLGEAILVPTRIYVKQLLPSIKEELLLGLAHITGGGLVENIPRALPKDLQARVDINAWEIPEVFKWFGRAGNVPVEDILKTFNMGVGMVLIVEKEKVQRVKELLQEANETVYEIGTLVKKSSPKDPGCVVENAVNLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1Y7</accession>
    <name>A0A1E5R1Y7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">D-lactate dehydrogenase (cytochrome)</fullName>
            <ecNumber evidence="6">1.1.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">D-lactate ferricytochrome C oxidoreductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g4171</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(R)-lactate + 2 Fe(III)-[cytochrome c] = 2 Fe(II)-[cytochrome c] + pyruvate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13521"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16004"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.1.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.2.4" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1Y7"/>
    <dbReference type="InParanoid" id="A0A1E5R1Y7"/>
    <dbReference type="OrthoDB" id="7786253at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008720">
        <property type="term" value="F:D-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903457">
        <property type="term" value="P:lactate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.45.10:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2740:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748:SF117">
        <property type="entry name" value="AER321WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="9">
        <location>
            <begin position="143"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00083446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="588" mass="64684" checksum="81A5E8168466D4BE" modified="2017-01-18" version="1">MLSQKLSKHIGTFSKQFPKFKPHFHLRQQSTLSKNNASFSKTFLPLVGGVAIGVGATLLYSTSQKKXEPAIEDSQNGAFLHKVLYNSTIKISDLESPKYGSEXDLQKAIAEIKTIIGDELVTNTESQLEHHTDNGYCPTKPLPEQVPKYIVYATSTEDVSEILQVAHKYSIPIIPFSGGTSLEGHFFTTRPGIVLDTSKMNKILEIHPDDLDAKLQCGVSWNQLNEHLSKTPGCENLVLGCDCGPGAHVCGMIATNASGVGAVKHGSMNNNVISITAVLADGTIFKTKQRPRKTSAGYNLTGLLCGSEGTLAVVTECVVKLQVQNPYETVAVVQFDDLKNCCKSVSAMFKKGLQLEAVEMLDANMMKAVNYCEQITETYDEVPTLFLKISGLNKVVVDELVKEVSKISKENNCTKFLFAKDKDEQEELFAARKNALYIMLDWGYGNIGDIKLWITDVAVPLSKVPDFAMKANKMVEESGLKSVTVAHIGSGNFHIDLYYLENQLERCEKLAKDISKLGIEMEGSVSGEHGIGLGKRELLPLELGPEAIDLMRKLKFSMDPKCILNPDKVIQIDXNEPYPPSKEPLMIX</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R260</accession>
    <name>A0A1E5R260_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">54S ribosomal protein L35, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g4116</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R260">
        <property type="interactions" value="245"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R260"/>
    <dbReference type="InParanoid" id="A0A1E5R260"/>
    <dbReference type="OrthoDB" id="2153661at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00866">
        <property type="entry name" value="PEBP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF82">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ80970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="374" mass="43124" checksum="93DF49DC99F3B04A" modified="2017-01-18" version="1">MLRRQLHTTTSKLSSXAQTTSASLKYPSQKLSEYFSAKQDSESLRPPSFRSKYFRNQYKSPLKIHETFDFCNEYLHSKILANRTSSSSEKVNPGSVAKDFYNPAVRYNFQFHDRIENSPQFIDYNLPVYRKLCLDHWQSSMDGKGQMLLXQRLESLKVIPDTLPTLEAPQCQLHVRFPFVNGVNRWIEPGMQLSTNATSFPPQFKIINMVPNMSISELSQKKYTLLIVNPDQPDVENDSFKTTLHYALTDVTFTDFNNNVLSEFNTKKGKPTFKVLADYVPPVPEKNTGLQRFAVWLLEHKDNKELLSEGTEVESILDSSNRLENNFDIRVFAEKHNLHAVGANVWRSAFDSSMETVRQQYGLPKGRVFHKVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2A6</accession>
    <name>A0A1E5R2A6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Lysine--tRNA ligase</fullName>
            <ecNumber evidence="3 11">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 11">Lysyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g4248</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>tRNA(Lys) + L-lysine + ATP = L-lysyl-tRNA(Lys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="3 11"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2A6"/>
    <dbReference type="FunCoup" id="A0A1E5R2A6">
        <property type="interactions" value="1236"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2A6"/>
    <dbReference type="InParanoid" id="A0A1E5R2A6"/>
    <dbReference type="OrthoDB" id="21243at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00775">
        <property type="entry name" value="LysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04322">
        <property type="entry name" value="LysRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000050">
        <property type="entry name" value="Lysine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000044">
        <property type="entry name" value="Lysine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00252">
        <property type="entry name" value="Lys_tRNA_synth_class2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002313">
        <property type="entry name" value="Lys-tRNA-ligase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034762">
        <property type="entry name" value="Lys-tRNA-ligase_II_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044136">
        <property type="entry name" value="Lys-tRNA-ligase_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018149">
        <property type="entry name" value="Lys-tRNA-synth_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00499">
        <property type="entry name" value="lysS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001756">
        <property type="entry name" value="PRK00484.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918:SF9">
        <property type="entry name" value="LYSINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918">
        <property type="entry name" value="LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039101">
        <property type="entry name" value="LysRS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00982">
        <property type="entry name" value="TRNASYNTHLYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="13">
        <location>
            <begin position="253"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="13"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ81029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="593" mass="67955" checksum="D4B8B19FA61FC93F" modified="2017-01-18" version="1">MSEQVAEQIANLHLDEVTGEKVSKSELKKRIKQRQKEAEKAKKAAANPSSKQNGVGAGGSKKKVVDAFADLSPSQYFEARSRQIKELRQSQSPNPYPHKFNVSTNLPDFLEKYKHLKRGETLPEEKVTIAGRIHNKRESGSKLKFYVLHGDGAEVQIMSPLQDYSDALAYEKDHDLLKRGDIVGVEGYVGRTQPKKGGEGEISVFVSKIILLSPCLHMLPTDHFGFKDQETRYRKRYLDLIMNKNARNTFITRSKIISYVRRFLDEKNYIEVETPMMNVIAGGATAKPFVTHHNDLDMDMFMRIAPELFLKELVVGGLDRVYEIGRQFRNEGIDMTHNPEFTTCEFYQAYADVYDLMDMTELMISEMVKEICGSYEIQYHPNPHNPEEVMNLNFARPWKRINMIEELEKILKVKFPNGDQLHTKETGEFLQKVLKDNKLDCAPPLTNARMLDKLVGEYLEDQSINPTFIFGHPQMMSPLAKYSRDVPGLCERFEVFVATKEICNAYTELNDPFDQRDRFEEQARQKAQGDDEAQMVDETFCNALEYGLPPTGGWGCGIDRLAMFLTDSNTIREVLLFPTLKPDTLKEDLEKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2D6</accession>
    <name>A0A1E5R2D6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Palmitoyltransferase</fullName>
            <ecNumber evidence="13">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g4119</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DHHC palmitoyltransferase family. AKR/ZDHHC17 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="13"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2D6">
        <property type="interactions" value="591"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2D6"/>
    <dbReference type="InParanoid" id="A0A1E5R2D6"/>
    <dbReference type="OrthoDB" id="6781668at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161:SF85">
        <property type="entry name" value="PALMITOYLTRANSFERASE HIP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 12" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="13 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="329"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="358"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="399"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="527"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="579"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="112"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="146"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="218"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="251"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="14">
        <location>
            <begin position="479"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ81059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="765" mass="87519" checksum="ACCCE5817949156D" modified="2017-01-18" version="1">MGEETENLLDTQDNSFIEQHSLHSDIDLHSQKHEQASLLNETEHDASSLKAIVSTGELETTNMNTSDLLSQPQEDPVIAKYRTACQNGDLRTVMELVDSSVVDLNEDSNPSDKVTGLHWAAINNKLSIVKYLISKGANVNAMSEGSNATPLHWAAKYGYVYIADYLLQKGADPSLLDVQGFNILHLATLSSNIMLVLYILLFVVDTEKKIDINATDKDGRTALLWAAYQGDLYSVQALLKLNASVNIADNLGFTPLHWAMVKGQPKVMKELIKYGSDLKAQTNEGKDIFVIGKEMKTETSLKIAFAENGLDEFGVLKKKWFKNPDHAKMITFLIPFFYLGITFKVCSCWSFLGGLFLVAXPLFVLSLIFLSTFVAPSFINKVGLTKREMFRTICFKSPLFSGILGGSILWAICLHFTRIVLHKKXLGWFYFLNSFVFLYCLLPYLFYKVMFLDPGYVDPYESFDKVRXDIKDLLTSGTFNSEKFCLDLWIRKPLRSRYSHISERMTLRFDHYCIWLYNEIGLNNHKYFMFFLVVLEISIFLMVKGSWAYFDYLEHHHISKCIGLLGDDELCAAYKKDRFMLFMLGWFFFQCLWVTPLLAMQFIWTMKGCTTFEFEDMQRKRKQKQLPGRDQDFQIFETAPRDEFHHSALENSVEEDTGGNQVLPKPFCLQKLESTAGIGGCLRLLGISNFFRIFNSKKSLRTVYVPTDYGXKTNLKDFWLTADGSAPLWMRFFALPFNKHTALLNGVSTDFATLYEYPEKEPGQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2H1</accession>
    <name>A0A1E5R2H1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mitochondrial chaperone TCM62</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4215</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the chaperonin (HSP60) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2H1">
        <property type="interactions" value="121"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2H1"/>
    <dbReference type="InParanoid" id="A0A1E5R2H1"/>
    <dbReference type="OrthoDB" id="4056908at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001844">
        <property type="entry name" value="Cpn60/GroEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45633">
        <property type="entry name" value="60 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ81107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="585" mass="66657" checksum="B21A78B54F43BAD3" modified="2017-01-18" version="1">MLPRTTLLRASSTSTSKATFDVFSKRCIKTLHTPVIKVPDDKRHLMLNHLITLNQILSKTQYNRNILYEDKYKSHPQMISSKDNYKLQKLIENIVMNWEKQEIKSKDRSEEVKLSKIGLRLFMDSCSSFKNADFLIATDLSIXLLKLYIRSPNKKDTLDEIRSLLESMLNYLNDKKIFIHENPAKIDSLIATLYPQHKTLLKKVLHELNYQLNEDDIVRVVRGKTFQDHIETNKGWKYNCGLKDDNNRYLSSLGLLDTSGQKNKKLVTINEECLVLFVDDKVLRDVNKVLPTLNYISRLDKPLVIFTSGNVFGDALNAISMHNNKSKRQNKKSQVLIFQYFDTSNSGLKLGENMDFINFCQLPKGQQSIYSQKFSEFTPSKITSDQYYGKLDSIKATTGEAFLYNDKHNIEENSNLKTTITLSVGGTNEFEIDQKRMELDYILNDFLAQSIKSGVVPGLGVSLVKAVAHSVLDVASPSTMQHHKQEYINIFSNSMANSIVQKFQADQFSANGRVSQTLYSNKQAAMDEAFVDDNFKPISMSEHGYLDPYNKLGGTLQAVDTFLKMACSCDTFVTKVYDTGKKSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2H8</accession>
    <name>A0A1E5R2H8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Dipeptidyl aminopeptidase B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g3908</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S9B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2H8">
        <property type="interactions" value="317"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2H8"/>
    <dbReference type="InParanoid" id="A0A1E5R2H8"/>
    <dbReference type="OrthoDB" id="16520at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000003">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002471">
        <property type="entry name" value="Pept_S9_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF200">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 10, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00708">
        <property type="entry name" value="PRO_ENDOPEP_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 16" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="15">
        <location>
            <begin position="160"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="14">
        <location>
            <begin position="619"/>
            <end position="820"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ81099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="826" mass="94106" checksum="28AE60E0B5EE9C10" modified="2017-01-18" version="1">MLFGKKKTTYESLHNTADELSHEALINKKNTDIAYWKYLVLVIILIYGSTLLISSINRLTCPQGFSGRKPIARNDTPAYGLSKVSFENVRNKTFTPEYKPLQWISTSDSLYDDRGLFVTFENEEYVVKSVFDLDYKKLLLKGKTFDVDGLNFTVDSFVSSPNLSRVLIRSQTVKNWRHSTFGTYFVYDVEKEVFQLIGKNIAHCQWAPNSDLVXYVFENDIYLXSAHXDKXVKRVTTDGSTQLFNGKPDWVYEEEVLEGDSALWWSPNSRYVTFYKINETEVPEYSINYYVQQENASYPEMKTIKYPKAGFPNPVVDLETYDXLADETLHFPLEDPSYIVNEVVWISDSQYLAKITNRYADDLYVHLIDVVDKKDVVTRSENAGTGWHELTHYTRHIPQNKTLGRPNNGYLDTIAINGWNHLVYFANAGNSNYTILTSGDWEVVGGACAFDYSTNDVYFVATKKSSIERHLYVVNLMEPLVITELTETAKEAWFSASFSSGGRFAVLTYGGPKVPYQKVIDFKSQKTDAIMDGNVAGETLFYLERNEKLNSAISKYDVPKKSFYELNLGKDESGKSIIVNSYEILPNNFNPKLKDYYPVFFFMYGGPNSQQVTNSFSVGFNEVVASQLNAIVVCVDGRGTGFKGKDFRSVVRDNLGDYEARDQITAAKIYGSKSYVNAEXISLFGWSYGGYMTLKTLEKDAGDHFKYGMAVAPVTDWKLYDSIYTERYMHTPELNPQGYNSCKVNDVKSIAKAKRFLLCHGLGDDNVHFQNMAQLMDKINLANVENYDMMVFPDSDHSIRYHNANNIVFDKLLSWAGAAFSGEFXR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2L2</accession>
    <name>A0A1E5R2L2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">MIOREX complex component 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3990</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2L2"/>
    <dbReference type="FunCoup" id="A0A1E5R2L2">
        <property type="interactions" value="253"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2L2"/>
    <dbReference type="InParanoid" id="A0A1E5R2L2"/>
    <dbReference type="OrthoDB" id="391988at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01876">
        <property type="entry name" value="YihA_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052279">
        <property type="entry name" value="EngB_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030393">
        <property type="entry name" value="G_ENGB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46498">
        <property type="entry name" value="GTP-BINDING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46498:SF1">
        <property type="entry name" value="GTP-BINDING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51706">
        <property type="entry name" value="G_ENGB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EngB-type G" evidence="5">
        <location>
            <begin position="119"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ80803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="303" mass="34585" checksum="490BBF73824C4EF9" modified="2017-01-18" version="1">MFLSTKILYQNGLKSVARTNYIATVNKAQAILEQRKIEQSGLSSKFRKKNSKQEEDTQVFYRFLQKLENRYNTEYKPASESAIQACNGIIKSLESKFEFGAPQYEIFEQKDSNTYQNKILPEIIFLGKCNSGKSTLLNNLLTNGKKKDLTKLARASKVAGYTKMLNVYNISNRFKIVDSPGYGVKGTAEQGDVTMNYLLKRKNLRRVYLLASAVTGLNHYDSAIIDYLIEHAIPFEIVWTKMDKVTPSLEPILLDNIDKSGVLEMPVKPRFFFINSKLNKQKCPKRYGLENLKVSIMQACNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2Q1</accession>
    <name>A0A1E5R2Q1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sodium/hydrogen exchanger</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g4157</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2Q1">
        <property type="interactions" value="363"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2Q1"/>
    <dbReference type="InParanoid" id="A0A1E5R2Q1"/>
    <dbReference type="OrthoDB" id="196264at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004709">
        <property type="entry name" value="NaH_exchanger"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00840">
        <property type="entry name" value="b_cpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF187">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01084">
        <property type="entry name" value="NAHEXCHNGR"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0050">Antiport</keyword>
    <keyword evidence="6 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0915">Sodium</keyword>
    <keyword evidence="8 9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184576" description="Sodium/hydrogen exchanger" evidence="12">
        <location>
            <begin position="19"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="165"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="269"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="391"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="475"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="13">
        <location>
            <begin position="89"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="532"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="557"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ81158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="606" mass="66705" checksum="88F446AC4F4D863E" modified="2017-01-18" version="1">MVSLAYFALTLLFQTAKALIEIDDGDNSDELAPNIPSKTLSGGSGVATGVPTSILNPGDGDDGGSVSDEIFSSWALFILLFLLISALWSSYYLTQRKIRAVHETVLSIFYGMIIGLVIRLSPGHYIQDSVTFNSSYFFNILLPPIILNSGYELNQRNFFNNFGSILMFAIPGTVISAVVMGIIIFVWTSLGLEGIDISFVDALSVGASLSATDPVTILSIFNAYKVDPKLYTIIFGESLLNDAISIVMFETCQKFHGKPTHFTSFIEGMGSFVMTFMVSLSIGLVVGIFVALLLKHTYIRRYPQIESCLVLLIAYESYFFSNGCHMSGIVSLLFCGITLKHYAHYNMSRRTQITVKYIFQLLARLSENFIFIYLGLALFTQVELVYKPMLIIVTAISICVARWCSVFPLSHVVNSFYKYKTRRSMIRNGDGTNIISNSDIPEEIPYQYQMMXFWAGLRGAVGVALAMGIKGEYKFTLLATVLVVVVLTVIIFGGTTAGMLEILNVKTGCIDESAEDSDDEFDIEAPRAIHLMPSGSNSNLPSPQMGPYSDSDINIRPSASPSNLNDNLSPETGPIKTSLSSIFKGGFHNFDEQVLKPVFLDQPKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2Q6</accession>
    <name>A0A1E5R2Q6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">tRNA (Carboxymethyluridine(34)-5-O)-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g4187</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2Q6">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2Q6"/>
    <dbReference type="InParanoid" id="A0A1E5R2Q6"/>
    <dbReference type="OrthoDB" id="271595at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106335">
        <property type="term" value="F:tRNA (5-carboxymethyluridine(34)-5-O)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051422">
        <property type="entry name" value="AlkB_tRNA_MeTrf/Diox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13069">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13069:SF21">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="5">
        <location>
            <begin position="48"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="213"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="176"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ80853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="284" mass="32962" checksum="9DBAADEAE8080D51" modified="2017-01-18" version="1">MSIEESKEEEFVHKVYNEIASHFSETRYKPWPIVTRFLEERTTGSVGIDVGCGNGKYLNINPNIILLGSDRSSGLIECAHNIDPQKYNVLVADGMHLPHQDNRFDFAISIAVVHHWSTRERRIXAIKHIMSKVKXGGEILIYCWALEQKDSRRGYDSSMEQDVLVPWVYQDKSHFTNNSQKTPTTIGPKKSEKPDLTNIAPKDRAKYVQEWKLKKQQERIAEQEKQKQEEELAKAEQEKKDQTKYRYYHLYKEGELEEDCVSAGGVVVLSGYERDNWYCVARKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4M8</accession>
    <name>A0A1E5R4M8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g3495</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4M8"/>
    <dbReference type="InParanoid" id="A0A1E5R4M8"/>
    <dbReference type="OrthoDB" id="3972902at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13145:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCHF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13145">
        <property type="entry name" value="SSM4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="422"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="619"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="644"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="686"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="724"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="787"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="827"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="883"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="14">
        <location>
            <begin position="35"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ81828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="933" mass="108379" checksum="544EE78CDB61199C" modified="2017-01-18" version="1">MSVSSSIHNEDLDENLQNEELIDINSHNDTTQVQDNLNDERICKICRGDEEPLFSPCNCNSHVHQECLLEWVKSKNGDINNGLIDYDNIVCEICKSKIVFKRSYDTAEASNVGVVEILRLKFQSLQQNKNMCIQLGFVVLGLLVGTLNWFTIRKYYLYGEIPSLRKITAYFFDESSNFIFNDLYLVLQCCIVVFFEYQHILNHESSKKIVNHHLQKQEDEELPPIWPSGSDISGLQALLFFPILVPAAFIAVGYIPVKAVQTLAPTLNLQASSSLFLFYWIVSLMVALVPVKLFSTKDERLFTRLFFTKYTCKEMTLSFFDYLAPLVIVKPVLDICFGNKLLHLNNYTFDIRKIDMFSLYGNPYTSLLNTVKLALXFANSHFYRYGLFSLIYDFQIHNAADTDHIFLFNVFHNLITHIKRMIFLLGATMMLYGTSLMVLVSYRPDVLPLSIGYGNSSSPLNSGKRSNSMIIANLDKLXDLASPLFVKFVQFYYKFFALKLARWFRLSEFLFRKTNIDERGSIVYRSLYHQWFKATXAQLSNTTLFSEPKTMSETHQLFKEEPTVHAYFVPNGQYRRVPNFLPKDITCEYYTPVTKNDKPLRPPKAKLENEYTPFRQYTVMYYFTLYTPPFYVLRLWAFHVCMGALHGVTALYFFISSNFVGQLILNRLRFVPWVSKKIEFGNNISFIKVFLGLLIQLMVINSVVYKDLTGPAVLFXSVFKIFRFPFFLNITLFPGVVLAFLAAGFVSYETTSAFTLAFKWKFLGKNPWPNYYFSYLLMPMYLMTXHLFIYVIPXGIXLAWLCLKLLIKIXIEDLQFKEGMKAVWNDYYRMFVVGFLKYILPALLIQFSVSAIRHCKFHKWDYFLLQXSIAIDFFSSNDSXLEYLYVLLGPLLFFGGLGRDSYRFIKAKYAKTKTEAIDKLHADDIXLENKKEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4P9</accession>
    <name>A0A1E5R4P9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3573</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4P9"/>
    <dbReference type="InParanoid" id="A0A1E5R4P9"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="435"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="641"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="796"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="863"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="184"/>
        </location>
    </feature>
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        <location>
            <begin position="185"/>
            <end position="217"/>
        </location>
    </feature>
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        <location>
            <begin position="246"/>
            <end position="292"/>
        </location>
    </feature>
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        <location>
            <begin position="350"/>
            <end position="400"/>
        </location>
    </feature>
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        <location>
            <begin position="435"/>
            <end position="446"/>
        </location>
    </feature>
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        <location>
            <begin position="447"/>
            <end position="465"/>
        </location>
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        <location>
            <begin position="491"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="801"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="878"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1236" mass="137870" checksum="4E5478BF6DD94D80" modified="2017-01-18" version="1">MFAINLGGSSSSSSRSRSSSSSSTGAGADRNEKRKNDDIGMNETMNKDMDNKDALNENKESKESMASKASMESKESKESVESKESKTNXLAESWENIGSEMYMSYHGNDKDDDDDDDDDDGSDGNSSDCSCDCSCDSEYSDDLSNTNNTNKKIKKKKKHNRKRTKKILESQSHSPPNNFNGNGILSSSSSSDNGSDEQQQQQQQQQQQQQHHLHQVQSLAQSFTNNGSSLSGXKHTRKGLVKERILSSQQSLKSTSQGTSLSFTSSSSGSEAALSSSLSSSPKSGSSPRLSPTHFLSSFVKKNNTTNNTTNHNNKKSNXENIANIQDKIVIPHRLSDVTDIQDFTSFVGNNKSNTNNNNNNNNNNIKDNNNNNNNNNNNNNDINNNINNNINDNNSNNNTEKSRFDDTQFANINALDEFKNEFGEYGYDAYVDYDDDDEYYSDQDSQDDRNTTTSGEKPERRDSGLYRLNSLKINKHSQRASSNSTMIVPTKSSTISSSTTNKNQHHHTQEDMKSTISSLSSSSYIMPVIPFDSKYASGSTIIGEDINTTTNIGSGTSTTDPTAVNNNGSVNAMHVSSVASSSIAENFANKAFIIPVFGPLKDLFYLKAPEGYKKMLVVENDITKVEIPIIIYSKNMDLESGNDTKRETNGNNKTLNKKPKSKRNFLKKWYGKQQPKINKGEHVCXSNEXEMNEKEETXFKTPSNCDKENINYLLGKFNELMLVPIIDSTSYSLXKSSTNQVRGQYEEYLKFLEPFYLNENLKILHQPVDITNNHEVFKLCRTLYKLKTEFQHQKVSISQSDRETADANXTDKKNESAANMEQEKGVANLGNTGELKVIADSNTRYQLYKIKKMIYGYHNIGQPKTQQNNNNNDDELLNYGLGDENGXNINNGEEENEEEGLDKTTLWIISVGLGLGLSITLYYFNINPLKAAIDFFRPEDSCVASSTITFVPASVSSFSCGGGESSGQSPVSLFAPKRHITNVYCYYTDNVPSIITVQLSKLSKWLQDADASQAVRNVSLATYSYVKTSGVVLVEYTDHVLNSLSRYYAELFDWLKKLIVENSPSTLPVQSSSGSLSNAVXSLTKTTTTLTRSIAEKTSTAVQLNCSDTSTALSTTRSMDLSPVYTKLNDFTQWVPKVYGSMQSQVAQHAHILAQNPVIRDMSIIAKHLWAFFQTKLHDWAYQIQQQDCTSNKVFETLSGTTTEVWRNVSDXYRNMNHVYHQHDQYSHIFQSIDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4V8</accession>
    <name>A0A1E5R4V8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Protein transport protein BET1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3694</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4V8"/>
    <dbReference type="FunCoup" id="A0A1E5R4V8">
        <property type="interactions" value="255"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4V8"/>
    <dbReference type="InParanoid" id="A0A1E5R4V8"/>
    <dbReference type="OrthoDB" id="261831at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15853">
        <property type="entry name" value="SNARE_Bet1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039899">
        <property type="entry name" value="BET1_SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12791">
        <property type="entry name" value="GOLGI SNARE BET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="39"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="7"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ81924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="129" mass="14437" checksum="83740886689C2F1A" modified="2017-01-18" version="1">MSQRLYNNSSSSSSSSSSSNLKNAGNNSKGLDYNGSMLSQLESQSEEQMSIMGTKVKQLKNLTTKMGDEIRSSNSTVSQLGETFEGTSLKLKKTFNKMMIMASKSRIPLRTWFLIFVVIGFLFFYVWVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R505</accession>
    <name>A0A1E5R505_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Arrestin-related trafficking adapter 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3617</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R505">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R505"/>
    <dbReference type="InParanoid" id="A0A1E5R505"/>
    <dbReference type="OrthoDB" id="2333384at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF62">
        <property type="entry name" value="ARRESTIN-RELATED TRAFFICKING ADAPTER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="2">
        <location>
            <begin position="400"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ81987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="754" mass="84172" checksum="BA93CBDD500621A6" modified="2017-01-18" version="1">MFSLQNSSYSKNYKQINNKEQHQSDKNHAKPSSGKPEDELPSNRPRKESSSITTYSNTSSSLPINASLTGGSSNSLNMQNPSYFDVRLKTNYKDMIVIAEDELEESKVFITGALVVSLIEPMSIKKISMQLVGNFKLDFIQTTGKHGYTTIVKETRPIFESLWDNLLNTSVGSITDLSAQKKKKKLSKKNALSLHLTNSNENLSSFXNPGRSSSPVNTDRPGLSTHGSATSLHGGDTGSPQHVKPYKKRSSSSASLWKKLGLDYISPGGLRSDSMNGLNSSNTGSFTQDGKRKIVKVLHPGDFVQGINSSCELENLNGIDYYHLETGNYEIPFSLEIPNNAMIPETVEGLQSGSVLYTVQATIDSVDKGKISNTKYIRVFKTLKVNNWAIHENLYVGQSFQDKLQYDIRLPSRAVPVGGESPISIKLFPFQKNYRIEKINVNMMQYYAVTDQHKQIFEEEQPIMQLTMQNFKNIDGIDPNTGVLIGDVHLRSTIRIPKNLKKITQDSTILENDTTTNTYKKLIMVRHQLSLRITLISPTNAVTEINCNMPIILYVSPYVPIETRRVVLDKATKIHFRPNETTPFFKSLLEPSMANKRQQGVFEGNANLIRYFPQEEFSAPPTYNERVTDRIIDGETINGLSHHIATAREMLVHRPLDMNSLQNINKTPSYDHTFYDSNNIATQQHNITATATTTTTSSASQEILGSEMTNGEQESMIGDTGDXSATAQHDGRANPYLPTGLFQFAADLSPEYQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R532</accession>
    <name>A0A1E5R532_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerases I, II, and III subunit RPABC2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3501</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the archaeal Rpo6/eukaryotic RPB6 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R532"/>
    <dbReference type="FunCoup" id="A0A1E5R532">
        <property type="interactions" value="697"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R532"/>
    <dbReference type="InParanoid" id="A0A1E5R532"/>
    <dbReference type="OrthoDB" id="259769at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.940.10:FF:000004">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00192">
        <property type="entry name" value="RNApol_arch_Rpo6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020708">
        <property type="entry name" value="DNA-dir_RNA_polK_14-18kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006110">
        <property type="entry name" value="Pol_omega/Rpo6/RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028363">
        <property type="entry name" value="RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036161">
        <property type="entry name" value="RPB6/omega-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006111">
        <property type="entry name" value="Rpo6/Rpb6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002208">
        <property type="entry name" value="PRK01099.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47227">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47227:SF5">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01192">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500154">
        <property type="entry name" value="RPB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000778">
        <property type="entry name" value="RpoK/RPB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01409">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63562">
        <property type="entry name" value="RPB6/omega subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01111">
        <property type="entry name" value="RNA_POL_K_14KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ82017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="159" mass="18215" checksum="421B271EFBD5C2C6" modified="2017-01-18" version="1">MSDVEQAYNEGPENYEDFDNEYFSDEEMADEVDAPTVKSEENGGVEKDSEGRVIITGGTQAEFEDVNHRRKTLKEKAISKEERTTTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRIAMKELAEKKIPLVIRRYLPDGSFEDWTVEELIVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R540</accession>
    <name>A0A1E5R540_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Topoisomerase I damage affected protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3542</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R540">
        <property type="interactions" value="97"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R540"/>
    <dbReference type="InParanoid" id="A0A1E5R540"/>
    <dbReference type="OrthoDB" id="10266980at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050846">
        <property type="entry name" value="TLCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439">
        <property type="entry name" value="CT120 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439:SF0">
        <property type="entry name" value="TOPOISOMERASE I DAMAGE AFFECTED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="25"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="7">
        <location>
            <begin position="68"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ81988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="286" mass="33442" checksum="CB19BCD3E366C044" modified="2017-01-18" version="1">MKDYLLPLSFYPNSTSLFYRNLHEIIGSFLMYSIIYQFLCDPIIKHLIFPRYPHYFSKLKSSQPKTFKEYLNFKIHIVAMVQCILSIILVSKNLFKQPLNLDITEYQDDYSTMVSSVTIGYFLWDLYVCIRWYSLFQXEFLVHALASLAVFVSTLRPFAQSWVSKFLIFELSSPFVNINWFISNILIKVLGVTSVPLWINALNGLMLISTFTVVRIFWGFSAVMILCYNVYAFGMYKNVNFSFSLMAMILIINFLMNCLNVFWLSKMLQIAKKMLMGSSKDGHKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R574</accession>
    <name>A0A1E5R574_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">60S ribosome subunit biogenesis protein NOP8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3836</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R574">
        <property type="interactions" value="188"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R574"/>
    <dbReference type="InParanoid" id="A0A1E5R574"/>
    <dbReference type="OrthoDB" id="21643at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="7"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="231"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="309"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="317"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="335"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="347"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ82042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="520" mass="61591" checksum="DFBB7DA192D4A2C4" modified="2017-01-18" version="1">MSEEIEKRIFVGNIHNNLHECCEELYKRFQKWGAVTSKSFEEHDHFAYVNVLFQNLDQFYKLKRQFNNVKFMGNTLKIDEAKEDFYARWIRENVNNEETALKEKIKENQLLKHEWEHYKKVQNIKMSWTDRRQVVFGRHRTHARSAKQLRKVTFRIFNERGELKVYKCYKNKLWGYDKKKTIRDLVFEFSNNKYWKDGNHHIIERLDFSKSKVFFANGDYLRISSKLQKSDTNGTVTSSASASHSENLQENFDEDDDSKLQKEHHVNAAVLDNILGSFNFEKPMDLNNGIEENEEYGSSDYEFSNKYLDQSSDDEENKPNEEADNTKIDSQVTTEHVEQKEIKEPPFAEEEEEEEEEEEEEEDKEVADEEEEEFIPSFGAPATGTVSNTETLRGLFANPEASTFKLIQEEDDDIDHEKDKEIEATLESEIXAVTSNFEEIPVQSKAKDKSIGLFFPHFDSYFLNGQTQLSKLKPKQVETFDNWDKEFWDHRAEWTKELRQKKRDAIRKWKKKNSKSTMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R579</accession>
    <name>A0A1E5R579_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GTPase-activating protein GYP3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3775</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R579">
        <property type="interactions" value="205"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R579"/>
    <dbReference type="InParanoid" id="A0A1E5R579"/>
    <dbReference type="OrthoDB" id="3971475at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000057">
        <property type="entry name" value="GTPase-activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000026">
        <property type="entry name" value="TBC (Tre-2/Bub2/Cdc16) domain family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF20">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="282"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="470"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="572"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="471"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="788" mass="90052" checksum="927065E880662F01" modified="2017-01-18" version="1">MQAKSMPNLLSGLKNSDRNKLFDKNDGIKNSYGNDTTNANSEYASSVINEYDNSTGNQQYESRKSVDQFSNLSLLDLYDTREGYTSQSDSLGMVNEDQDIEKEEYFNRDLTCSPSAEGQSNIHLIVXLENSVNTLDVNEXHVNNRFNETSANANANTNANTLPHSPITPTNKYRKPFPFLSIKNDELDHYDRYGFKKETSYITEQEYDAWWNDYSQYSARRKGKWIHFLEKNGIEFDTKTHVPKSYPMGGINNDNLNNNNSHNNETNTSSKAEKLKRYVRKGIPAEWRGESWWFFANGNEKLLKNKGLYHSLVGKLFDSNDHCVLPQETLLKNISSILKNDDFFTDVQIIERDLNRTFPENIHFMKDSEKEPSMIVSLRKVLYAFALHDPKIGYCQSMNFLAGLLLLFLDEEKTFWMLVTITNEILPNVHNRNLEGVNVDQGVLMLCIKEYLPEFWSKINNTPQDQYLKNSSSFSSESSSSSPFGKESPTPNKKNEFLFKLPPITLCTAGWFMSCFIGTVPIETTLRIWDCVFYEGSHFLFKTSMAIMKLSELNPSSTESFFKDNLSNNKKNGSSSSFNSIDSDNGTQQQLNNGQNNKSKNGSRSNSTSKNKRASLSSTNLNAXSTVASKNSTETHHPLRNSLLIRKFSKSKLKILSHSNTTIARKSSATANANKDDLYIELFQTFQTFPKTLLNPNDIFEKIVFKRKYHLNALNQDEIVRCRKYVIAQRAKYKKIQQLAVPANADVDTENTELNAIMQDDFYGFGYSTGTTLKERVMKITRKVESFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5A4</accession>
    <name>A0A1E5R5A4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3593</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R5A4"/>
    <dbReference type="STRING" id="56408.A0A1E5R5A4"/>
    <dbReference type="InParanoid" id="A0A1E5R5A4"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="526"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="20"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="95"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="165"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="301"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="339"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="402"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="617"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="112"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="165"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="209"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="258"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="301"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="343"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="417"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="453"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="625"/>
            <end position="642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ82084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="699" mass="75889" checksum="6F586F70DA1DAA42" modified="2017-01-18" version="1">MSVENQNSFRNSNVNTAFIRNASIKGKTKSNRDSKNKGKNDSFTQAQTPPLATTHTTNSHSNKHNNKVFNYGTPVRSFVDGDGLTSKDKKVTNILNTDSSSSQDEEMADVASSVSGKSNNSTDSLKSSVDSNDMVMMDDSEINNKDNTKNKLPSLSTVLDNINIGTNNTTTTTPNNTLQQSTMSSSNMRKHDLTSNPLGMHVIGTSLPSNQSGSNVLSGGNLSSNLSSNLSSNTSSNVNSLTDSNTDSVLESNSNGTNSHSGRSSISSGNSMSGNSNSISNTADHQFEQKYSQPKQSFFTVNNSLNESTGGSMKPPRTQPKTNSASNQYNLVPSYQTLLSSHNQNNNNNNNNNNIRPSNSIVSITSMNSGCGSPITMDRAKMQSTPQMDAQSFWNSQQDHNGTVNSSMSNGKGGVSMQPSNSLQSVSTTGSYNQFPKLNTSFGNGTQMSPQMNGSYTPSGFSSSNSSTSNVTNGDLMSPILSKYSINNPQQTVGSFATTPSLLPMGRKKLQNKDEFLAPKNVATTSTCHICGKIFDRKSWLERHLSCHNDMRVYKCLWCNSRHKRKDNLLQHLKAKHPVELIKALFEEMSANNYPQGVLNKFQERAAIVLQEVTNTEKGGSTAQTNDHNNNTTNTSSTISDIFDTEKNSDGSLEPHSVHNKIIKVLVREGVLSKDLLRNVLNTLVVKQADYADDIYHVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5A6</accession>
    <name>A0A1E5R5A6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADPH-dependent methylglyoxal reductase GRE2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3605</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R5A6"/>
    <dbReference type="FunCoup" id="A0A1E5R5A6">
        <property type="interactions" value="268"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5A6"/>
    <dbReference type="InParanoid" id="A0A1E5R5A6"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05227">
        <property type="entry name" value="AR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000191">
        <property type="entry name" value="Methylglyoxal reductase (NADPH-dependent)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="6"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ82095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="344" mass="37955" checksum="82F3B345955C809D" modified="2017-01-18" version="1">MSKPTVFISGATGFIAQHIIKQLLDSKTYKVVGSVRSAAKAEKLESDFGNHPDLSLVTVEDLSKLDAFDSVFKEHGASFDYIFHTASPVVFTITDFQNEMITPAVNGTKNIFEATVKYAPNVKKFVQTSSSAALRNPEASTDPSVNVTEKSWNTLGLEESTKDYIKAYFYSKGTAEKISWELHEKLGAKFELTVINPVYVFGPQAFDSSVSNHLNFSCEVINSIVHSKFNTPLSNSVKGNFIDVRDVARAHVEALTSDKLSGQRLILSENIFSSELIANIINEKFPQLNGKIAKPSPISNEEISKHLANIDNHVTKGLLSFEFRDLTTIVVDTVSQILNVEAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5C6</accession>
    <name>A0A1E5R5C6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">RING finger protein ETP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3609</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5C6">
        <property type="interactions" value="754"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5C6"/>
    <dbReference type="InParanoid" id="A0A1E5R5C6"/>
    <dbReference type="OrthoDB" id="273556at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16457">
        <property type="entry name" value="RING-H2_BRAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047243">
        <property type="entry name" value="RING-H2_BRAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24007">
        <property type="entry name" value="BRCA1-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24007:SF7">
        <property type="entry name" value="BRCA1-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="277"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="UBP-type" evidence="5">
        <location>
            <begin position="311"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="585"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="511"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="588"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ82099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="610" mass="69496" checksum="DFFCA171AAE1CE51" modified="2017-01-18" version="1">MSLPDYYSIKIELDSGYTTSIAYQIFHSACFLKAKPKKXRVKHINLKRKLIKLESVIDSAMSTSLDRESISSFDIFGSVFEGQIKLFKLSNSNVTYNQHSETLVKDEVVKKVVDENNVMACIIFEPQIMLLDVLKKIFPTGDSLKAVTHVRCLKQLSVQSEVKNSFTLLDESGEDQGYHDNLLLVNFTNTENKNQFSLEFDGYEIKQTLLKVVNIKEVVFTAKLFQTISLAHEGESSTKSXYNSPSFRNQPVLPYLIEDPFTKENNSFVSNIELPTCPVCLDRLDSATTGIITTNCQHAFHCKCLMKWKNSKCPVCRYTLDQTNRNEKKCNQCDARTNLWCCLICGHVGCSRYSSKHAIDHYKDSGHCFSVDLNTSRVWDYAEDKYVHRLVQNISDGKLVEISGTSNGFTGNQRDSVTSENAKKGISNNEDNLEYIQLLLSQLESQQTYYENKLSTIKQEKKKDLEVVCAKWEQKFKSLEQKAVASDLQFKNLHESIEVGKQTNVNLLTTLQAMKDEIKSLKTHNEKLEREKLEQEENTKDLMFHMEAMSKLNNLDGPLSSANSGANLAGHIVIKEATEANMNTLEAKKRKNKKKNEKKKLKKLQMLDQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5D1</accession>
    <name>A0A1E5R5D1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Malic enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3590</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="7">Divalent metal cations. Prefers magnesium or manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the malic enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5D1">
        <property type="interactions" value="899"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5D1"/>
    <dbReference type="InParanoid" id="A0A1E5R5D1"/>
    <dbReference type="OrthoDB" id="5365701at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004471">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05312">
        <property type="entry name" value="NAD_bind_1_malic_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10380:FF:000001">
        <property type="entry name" value="NAD-dependent malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="entry name" value="Malic enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015884">
        <property type="entry name" value="Malic_enzyme_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
        <property type="entry name" value="Malic_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010052">
        <property type="entry name" value="PRK13529.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406">
        <property type="entry name" value="MALIC ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406:SF34">
        <property type="entry name" value="NAD-DEPENDENT MALIC ENZYME, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000106">
        <property type="entry name" value="ME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00072">
        <property type="entry name" value="MALOXRDTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00331">
        <property type="entry name" value="MALIC_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malic enzyme N-terminal" evidence="10">
        <location>
            <begin position="83"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Malic enzyme NAD-binding" evidence="9">
        <location>
            <begin position="274"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="418"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="458"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ82117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="572" mass="63473" checksum="88B6E30E2232422E" modified="2017-01-18" version="1">MSGKLSLSVQGEIQCGLEGFELLNSPIFNKGSAFTEEERDIFKLNGLLPPHVDDLEKQIERAYKQLHDLRDPLRKNDFMTSMRQQNKTLYYALVNRHISELVPIIYTPTEGDAIANYSNRFRRPEGVFLDITQPDTILDRMSKFGNDSDIDYIVVTDSEGILGIGDQGVGGIRISLAKLALMTLCGGIHPGRGLAVCLDVGTNNETLATDELYMGNRFARVRGKQYDDFVEKFINVLKEHYPSSVLHFEDFGVTNARPILEKYKSQLACFNDDIQGTGAVVMSSFLAALKHTSRDLKTAKVLIYGAGSAGLGIADQIVNHMVNHGLTKEQARNNIYAMDRHGLILDSMGDVSYEAQMFYSKKDEDFKDXNTKSLVEVVGQVKPTCMIGCSTQKGAFTEQVIREMYKHNDKPIIFPLSNPTRLVEAVPEDLLKWTDFNALVATGSPFQPVEGYNIAQNNNCFSFPGIGLGAVVCKASTISDTMISAAVDQLADLAPINNGDPKSGLLPSLDLINELSSKIAAAVVLQAVKEGVAQRKDIPLRFEDTLKWVESQMWRPVYRPMKKTLGKYTHQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5E4</accession>
    <name>A0A1E5R5E4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Bud site selection protein 21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3584</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5E4">
        <property type="interactions" value="274"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5E4"/>
    <dbReference type="InParanoid" id="A0A1E5R5E4"/>
    <dbReference type="OrthoDB" id="4096107at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013268">
        <property type="entry name" value="UTP16"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08297">
        <property type="entry name" value="U3_snoRNA_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="242" mass="27445" checksum="DCA6A82483BF1866" modified="2017-01-18" version="1">MAKIKFGDEDASVXPLPESKPAAVSGNISKNNAIDTSSDEDDEAPEEETVADSAGQIQQLEKQQQDAIAQKENELKAKRRAKTLKFQEQQKLKTQKLQFEKEKLLEFEKQLKLKESETQKNTPTGAILNELAMEELPEDFLASLNSETGNEKSTAANHHKFDEDXENQVFDSTNDDYYESAQQIRKAKQNTLKNLRKLEVKKGSFLISRKPATQSKSVAPKVEKKVMSVRDKWLKRKALNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7A0</accession>
    <name>A0A1E5R7A0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3489</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7A0"/>
    <dbReference type="InParanoid" id="A0A1E5R7A0"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="671"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="679"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="713" mass="84208" checksum="FA0C58AF409A4C24" modified="2017-01-18" version="1">MQLVKKYELKEILRVVLGKKISHAKSVKYDFKRKQQFLQNRIGALEELHEYNEFTRGTPVYVAQELHKMLPMIRKWIRYDAHLGVEQTTGSTLQSSQSKQNKIPQLYTLTKSNQEFVRLNRADYTIPAVFRALFAQGYLMKDMKFNFHRQYTKEELIVESAASMKDELLVDYPRLYEGLVELQLEDPADKIVINWPFINDAMFQIKAIGVYNRPDLVAKYKSVSLLRKTIPLVNIKNKKLLVSRPETQNTFVEGVALLPNRIKEHLDEYLESFQPNDTIDLAEYKPNNLFSDKLEEASNVVIIHKPIKLCKTPPLNVYNRKTSFLNYKASKSNQAIFSKVTEGSEEQFFRMLEDYGTDEFRTVMLDIKRFDTDKFYQELDKEYWNINSLFKIYKTMEKQWFPLQLKNPLDMGILKDMTLCKWDHDILKSLHVLNSLNFLKDDRLNIRTEMHAFIKQLSNYYMISLAEIKSRNLDKKGYFYSRYRQIIFQNVLKFCFFENKWMANMYPNFTFSLNRSLKQEHDSVYHYIKNEIMSTKRKLSKPCSIENNESNLYKSVVENCVNMEYKFFTTHSGQESVDHGDQAIEQHQQFETRALHLKDLKTRYKIVLDFRKKRIVGEELKNSTLIKKFLDDEYFLTVLKNLTFLDVEVLGVGDYPSAEEPGPLLKDMRFSKHKKQPKKSNPSNPSHPSHPSLLEAWDDKITDAYKLYLCRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7C2</accession>
    <name>A0A1E5R7C2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">Glutathione reductase</fullName>
            <ecNumber evidence="3 16">1.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AWRI3579_g3318</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 16">Catalyzes the reduction of glutathione disulfide (GSSG) to reduced glutathione (GSH). Constitutes the major mechanism to maintain a high GSH:GSSG ratio in the cytosol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 16">
            <text>2 glutathione + NADP(+) = glutathione disulfide + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="13">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 15">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.7" evidence="3 16"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7C2">
        <property type="interactions" value="1068"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7C2"/>
    <dbReference type="InParanoid" id="A0A1E5R7C2"/>
    <dbReference type="OrthoDB" id="5956163at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.30:FF:000003">
        <property type="entry name" value="Glutathione reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000235">
        <property type="entry name" value="Glutathione reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006322">
        <property type="entry name" value="Glutathione_Rdtase_euk/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046952">
        <property type="entry name" value="GSHR/TRXR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01421">
        <property type="entry name" value="gluta_reduc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004776">
        <property type="entry name" value="PRK06116.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737">
        <property type="entry name" value="GLUTATHIONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737:SF2">
        <property type="entry name" value="GLUTATHIONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 13 15" id="KW-0274">FAD</keyword>
    <keyword evidence="5 15" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13" id="KW-0520">NAD</keyword>
    <keyword evidence="16" id="KW-0521">NADP</keyword>
    <keyword evidence="13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9 15" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="18">
        <location>
            <begin position="8"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="17">
        <location>
            <begin position="358"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="458"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="190"/>
            <end position="197"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="14">
        <location>
            <begin position="45"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU365016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OEJ82809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="469" mass="51632" checksum="4A2B78C1CE98EB6A" modified="2017-01-18" version="1">MAPIVKHYDYLVIGGGSGGVASSRRAASYGAKTLLIEGKAMGGTCVNVGCVPKKVMWNASDLATRVQQANEYGLYRQFPLNKENLTFDWAYFKEKRDAYVHRLNGIYERNLKKEGVDFLYGWAKFNKDGQVEVTHSEDPSXVSTFTADKILIATGGQAILPEKVPGYEHGIDSDGFFKLTRQPKKVVVVGAGYIGVELAGVFHGLGSETHLVIRGETVLRKFDEIIQECITEHYISEGLTIHKTSQVIKVEKNESXGKLTVHMDTGIILEDVDDLVWTIGRKSLLGIGLENVGVKLNSHDQITVDEYQRTNVENVYSLGDVVGKVELTPVAIAAGRKLSNRLFGPEKFKNQKQDYENVPSAIFSHPECGAIGLTELEAVAKYGKENVKAYTSKFTGMYYAMLEHKSPTRYKLVCVGEDEKVVGLHLIGECSSEILQGFGVAVKMGATKEDFDSCVAIHPTSAEEIVTLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7F7</accession>
    <name>A0A1E5R7F7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerases I, II, and III subunit RPABC5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3374</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the archaeal Rpo10/eukaryotic RPB10 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7F7"/>
    <dbReference type="FunCoup" id="A0A1E5R7F7">
        <property type="interactions" value="523"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7F7"/>
    <dbReference type="InParanoid" id="A0A1E5R7F7"/>
    <dbReference type="OrthoDB" id="10258858at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000024">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00250">
        <property type="entry name" value="RNApol_arch_Rpo10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023580">
        <property type="entry name" value="RNA_pol_su_RPB10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020789">
        <property type="entry name" value="RNA_pol_suN_Zn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000268">
        <property type="entry name" value="RPABC5/Rpb10"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003089">
        <property type="entry name" value="PRK04016.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431:SF3">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01194">
        <property type="entry name" value="RNA_pol_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005653">
        <property type="entry name" value="RNA_pol_N/8_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46924">
        <property type="entry name" value="RNA polymerase subunit RPB10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01112">
        <property type="entry name" value="RNA_POL_N_8KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ82842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="71" mass="8397" checksum="61DFE7826927AAE3" modified="2017-01-18" version="1">MIVPVRCFSCGKVVGDKWESYLNMLQSEEEIDEGTALSRLGLKRYCCRRMILTHVDLIEKFLRYNPLEKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7G7</accession>
    <name>A0A1E5R7G7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcription factor SMP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3316</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7G7"/>
    <dbReference type="InParanoid" id="A0A1E5R7G7"/>
    <dbReference type="OrthoDB" id="3972009at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945">
        <property type="entry name" value="MADS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945:SF534">
        <property type="entry name" value="MYOCYTE-SPECIFIC ENHANCER FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="103"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="488"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="703"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="885"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="128"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="191"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="225"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="243"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="285"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="492"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="713"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="733"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="756"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="886"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="927"/>
            <end position="938"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ82852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="938" mass="101510" checksum="F11BC07DCCE0DB48" modified="2017-01-18" version="1">MGRRKIDIEPITNERNRTVTFIKRKAGLFKKAHELAVLCNVDIAVIILGTNKQFYEYSSVETPQLINTYQRKDLQHHVKDPCDYGDGFVKKKFIDVQLSPGKFKNRKPKKKVGQEAIQEHTDHIDVEAREEDEEGPEDEKEGEGEEGEEEEEEEEGEEEEKEEGEEVEEYTLDQLGKIDSKMGNTKKLTKRQATMLQDQVSSNTNKKRKLSNPVSQGKNPRKSTKNSGGSTNTSADEQETVLKTKSNIKSSNKTQNKKQLRIKTGEKKLDSKMVQQFQQMYSTTLSKSNDTTPNQQRHTVPPVSAVPSFPTNTAHQNPAYLGSQTITVPAYHQQGQHQYQSLSGYSGKPFLYPGSATANTATSLRTGSLNVNNTETDVSKPNLFAPNANNTLVSRLDSSSFLANTGMLRGNRTPTTPALLIGGDNLMARPFGKGNLLTPKNTTFNTSNDVTTGVNEDIKNSRKLSASKLKDQNNLYPSSRPQLRVEIPNTGRDLQSSDSSTTENIGVSGSIAESNANLDESTTENANVSGNHVADANAGGTNRPYPGVNYGISVHANSHKTPVSSALPSPLLFDSATKLPNVSSIPFSSKQPSFNPAATRVRGNNFFNMSVQNGNNNGNSAFPSSAANSNIPTNYMPQSAMISPSFQQYFATPLVGPGAYANANGAQLNANISNKNTDTNINNTTTDPHSANSADEIIKEKKMGKQVHTKKPSLTVNTSSTSLESTSYSNSENQPLSATTKTLIGDQSFPERPNIKTGNTKNGGSFQHNKNFDSANPYAFKPHLPHINTAMDASSNTAPTSVGNPDTVNVDQNGAGLNANGGGLSAVFSPMNYLFQDWGQPPSALPSNTGFPTAQANTLSGLLHNNRNNGQASIASANAANVSHNDYPTLSTANMNNTHVEKPNASGAXPDKKTSTAGDFVLSKDAPTTDKDKENKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7Q4</accession>
    <name>A0A1E5R7Q4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">tRNA (uracil-O(2)-)-methyltransferase</fullName>
            <ecNumber evidence="3 11">2.1.1.211</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3236</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Adenosyl-L-methionine (AdoMet)-dependent tRNA (uracil-O(2)-)-methyltransferase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>uridine(44) in tRNA(Ser) + S-adenosyl-L-methionine = 2'-O-methyluridine(44) in tRNA(Ser) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43100"/>
            <dbReference type="Rhea" id="RHEA-COMP:10339"/>
            <dbReference type="Rhea" id="RHEA-COMP:10340"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74478"/>
            <dbReference type="EC" id="2.1.1.211"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the TRM44 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.211" evidence="3 11"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7Q4">
        <property type="interactions" value="99"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7Q4"/>
    <dbReference type="InParanoid" id="A0A1E5R7Q4"/>
    <dbReference type="OrthoDB" id="10047021at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141101">
        <property type="term" value="F:tRNA(Ser) (uridine(44)-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011671">
        <property type="entry name" value="tRNA_uracil_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21210">
        <property type="entry name" value="TRNA (URACIL-O(2)-)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21210:SF0">
        <property type="entry name" value="TRNA (URACIL-O(2)-)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07757">
        <property type="entry name" value="AdoMet_MTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7 11 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 11" id="KW-0819">tRNA processing</keyword>
    <feature type="unsure residue" description="D or N" evidence="12">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ82945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="642" mass="73975" checksum="DF17EA62AABF980B" modified="2017-01-18" version="1">MVDIETVVENNASHQQQQQQQQQQNHEQMFVSAEFDFVGDQMLKDGMSKMDYDLPINVNVKDYHEDVKVIGNEEQIENNTNSQWQPSWITLFQSKXCSNDSSLKLPFDKNHFIQAMTNVIKHPNINSTIILRADLLVEVEQSRAIEXNKDLIETDFNNDPTINYITINTQDTAFRDINLHGDNEKWHLLKGYVRRIIPRNPSKDAIINQSCLVLEHANKQDVLIIYTPHIQNQEQCPYYIPKVAAVGILFQPNSLSVHYIPFAEMMPQLNSLETNIFTDQQQRIVRTALRLLQTAYKHSKGCKNGYSPKVKHDLIVDKVKFQKKYLELKQKYGSYLVENWMESTDPKKHAFEEIAIAAFLLELWDLKYKGKYLANNSDFEFRDLGCGNGILTYILIMEGCNGLGIDARTRKTWQMFPEKVQQHLKQQVIVPSILLRPNIQMKYLNPNLTDNNMVFQIPAHNDLISDKATILYTSKDLIESKHINIAEFTTTANASFENIFVIGNHSDELTCWIPLLGYPFMVIPCCSHNFSGAKHRFRQKQFASKTNNNSRKDPQQSSLSTYGALVEKVIDVSKRAGWDPVEKEMLRIPSTRNAAVIGINNTNYNGKLNRLNWPNETVYNIIMEDXGANGWIENSMKLMTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R825</accession>
    <name>A0A1E5R825_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">DNA topoisomerase</fullName>
            <ecNumber evidence="3 7">5.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3172</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="3 7"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R825">
        <property type="interactions" value="935"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R825"/>
    <dbReference type="InParanoid" id="A0A1E5R825"/>
    <dbReference type="OrthoDB" id="430051at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031422">
        <property type="term" value="C:RecQ family helicase-topoisomerase III complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
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    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.290.10:FF:000001">
        <property type="entry name" value="DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="entry name" value="Topoisomerase I, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="entry name" value="Topoisomerase I, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000380">
        <property type="entry name" value="Topo_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023406">
        <property type="entry name" value="Topo_IA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390:SF21">
        <property type="entry name" value="DNA TOPOISOMERASE 3-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390">
        <property type="entry name" value="PROKARYOTIC DNA TOPOISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="Prokaryotic type I DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00396">
        <property type="entry name" value="TOPO_IA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52039">
        <property type="entry name" value="TOPO_IA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="9">
        <location>
            <begin position="3"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IA-type catalytic" evidence="10">
        <location>
            <begin position="209"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="316"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="604"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="316"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="328"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="604"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="615"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ83057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="715" mass="81197" checksum="06BBABC3DAA8F355" modified="2017-01-18" version="1">MVKLVCVAEKNSIAKQVSAILSGSSATTRNSPNKYIKNYDFKIFNPGYLFPFLNIEYPMDVTMTSVXGHLSSLDFIGNDGPSSTRGSNSSNKGPYAWGNCDPIKLIQEAPLINSVPQKDVALNIHKECANAQYLMIWTDCDREGEYIGWEVFMAANYGKNFQKFFVNVPQSSXLHPSLKFNEKNTFRANFSHLEKQHIINATRNASKLNQNLIDAVATRMEIDLRSGLSFTRLLTNYFKSNGLVSKETVVSYGPCQFPTLGFVVDRDDRIAKFVSENFWYLTLTVYENVKFQWCRNYLFDRLSVLQIYDMCVSSNATTTSNSSTNTQHNDDKNNKETQDDLSPSDYVVVEEVKNSTIKKFKPLPLTTIELQKNLSKFFKFNAKKSLDLAEKLYQKGFISYPRTETNIYPATMDFHKLIEQQLQTSSQEIKNYCSTLLNDGKFEKPRAGKNDDKAHPPIHPILSCDLSSLPHEEARVYEYVVRHFLAGCSKNATAQQLSIKVKWGVHLDXPEFFKTSSVQVKERNFLDIFPYWDWKSNVLNLSXDVKPGDKLPIQETDMKMGSTSPPKPMTESELILLMDANGIGTDATIADHIEKIKARTYITQEKSSASGSRSTETDPTSRPETNKRNSTNGGVIYLRSTKLGKSLVHGFDKIGLRDSFTKPFFRSDIENFLKKISNGEIHKPLVLQQSLDKYLEYYSITNAKKEILKKVYTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R830</accession>
    <name>A0A1E5R830_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA polymerase</fullName>
            <ecNumber evidence="11">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g3441</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="11"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R830">
        <property type="interactions" value="696"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R830"/>
    <dbReference type="InParanoid" id="A0A1E5R830"/>
    <dbReference type="OrthoDB" id="2414538at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016035">
        <property type="term" value="C:zeta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05778">
        <property type="entry name" value="DNA_polB_zeta_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05534">
        <property type="entry name" value="POLBc_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000007">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000024">
        <property type="entry name" value="DNA polymerase zeta catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056435">
        <property type="entry name" value="DPOD/Z_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030559">
        <property type="entry name" value="PolZ_Rev3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056447">
        <property type="entry name" value="REV3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45812">
        <property type="entry name" value="DNA POLYMERASE ZETA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45812:SF1">
        <property type="entry name" value="DNA POLYMERASE ZETA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24055">
        <property type="entry name" value="POL3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24065">
        <property type="entry name" value="REV3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 11" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA polymerase zeta catalytic subunit N-terminal" evidence="15">
        <location>
            <begin position="11"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase delta/zeta catalytic subunit N-terminal" evidence="14">
        <location>
            <begin position="147"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="13">
        <location>
            <begin position="739"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="12">
        <location>
            <begin position="922"/>
            <end position="1377"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="16">
        <location>
            <position position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF24055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF24065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ83065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1532" mass="176817" checksum="F75B7D588BE7CF08" modified="2017-01-18" version="1">MSSQPQEDSALKVQLNSYDIYEIEPSKELDANGNGEYTYEQVPVIRIYGSLSSGHNIMMHVHGVFPYMFIKYEETTLANITKNCSHLHDRLEKLLDDYYSSSKGKKKNDKKGKQGDIDFEDDLNGAEVDGNTHVNCTITKKTRMQDVKEPVKKKVYKYIARVEVVKGVPFYCYQVGWHLFYKISLLKQNTMNRLATILRTSKNLFEKKTVNKQQQGMSTDDIKVFEAHIPYFTQFCFDYNLYGCSWIDLSNCYFRFPLLNNQFGLDQLWSKRDLEETLFAQKYEHQYLNTDSYPRMSNSLLEIDVLASFILNRTQLRFEDTHSKIHETSSQDGLSFKNSVSSTEQLWRKMDKQRERLNLSPYLSSALTNTDVAPPLWSDNKTLWENFDRLLKENSSKRQTPLFFDSSFYKHIQTASESVKTLWPKEFEDTSFFTTPFRTSTSSNGRNFGKGLLSSQGSFLEESSFDVSEILARTNETSAPSLARSTHLHKLNKLTRETELIAEQEKIKRKSSFWENPTISHKFLKKRAEANRIDFSNDLLSLYEYKPRALNHKNLNESFESLGIQKIEYKEPFFSNPKDFNEDKKYEYSGKTFPINCTHVRHRKLITFRHTSFERKISSSGTAEKYIYIPEPPSYYRVLESASNSQLLEDGSQIVIAGSNNTKYKYQSFEKSESQSKKSSFYNNLSQLSVEVFVTTTNDLYPDPLKDPVNIIFWRYENDNVSLNYVTEGLLCFEKQASKYQKLYQCCKFQNLKVVFFDDERSLFDALVELVQCLDPDILSGFEIHASSWGYVIKRASAAFDFDIAQELSRVSFKAINKVGDRWGYNHASAIKIVGRHMLNIWRILRKELNLLSYTIENIVYQVLHKRIPHFSYRSLSEMWQTDNFDVVINYWKSRIDFNIQLLGNIDVLPKITEQARLIGIDFYSVIYRGSQFKVESIMGRITRSENYIMLSPTRKEVNTQKPIESISLVIEPASGLYTSPLVVLDFQSLYPSIMIAYNYCYSTILGDVRLMKSNSKTKIGVNDNYCLQENLLNAIGAENINIAPNGVMFVKSSVRKSVLAKMLTNLLDTRIMVKSLMAQLKKHAAGANLVRDLNSMQLALKLILNVTYGYTAASWSGRMPCQEIADAIVETGRETLLKAIKQIEANDKWGAKVVYGDTDSLFVYFPGKTKAEAFAFGREMADAVTNSNPAPVKLKFEKVYLPSLLMAKKRYAGNMFEFEDQLTPKLDAKGIEIIRRDGNPAQQKMVEQCIRLLFSTKDLSQIKAFVQNEFSKIMCGKVLVQDFCFAKEVKLGYYKSEKTMPPGAVLAKKLMEQDNQNEPQYKERVPFVVVKGKKNDLLRDRCIPPLQYLQNMDKFQLDSDYYIIKTLIPPLNRIFNLMGVNVDEWWKEIPKNQSLDDFSSNTIVKQVKLNACLRCGKASSRKICFSCTSDKEYVQEIYPIAKRIEKMDNTLKVCRQCVAVNYCNNNNAKSSNFIHQCNAQTCSVYYQRYKACYMLSNANPRVEIQTITTQESTEKDNLVELSSSFDFDIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R893</accession>
    <name>A0A1E5R893_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Proteasome maturation factor UMP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3334</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the POMP/UMP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R893">
        <property type="interactions" value="407"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R893"/>
    <dbReference type="InParanoid" id="A0A1E5R893"/>
    <dbReference type="OrthoDB" id="15001at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008012">
        <property type="entry name" value="Ump1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12828:SF3">
        <property type="entry name" value="PROTEASOME MATURATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12828">
        <property type="entry name" value="PROTEASOME MATURATION PROTEIN UMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05348">
        <property type="entry name" value="UMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00043974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ83114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="158" mass="17866" checksum="84A90547C3663D7D" modified="2017-01-18" version="1">MNFIPGSDFKSSISTHQTTHTSNAVPSLPDKLRSQDGGCVPMQMKELSNAGKHPLESRITNWNNQQEQLKLQQYKQIFGLAEPMKRVXEAKIIDSTDSFKPLLTDSNATSIHQDIVKNKEFDIEWEDVYNPSTNPDCLSTFNTINNNVHTKIENHLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R8P8</accession>
    <name>A0A1E5R8P8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sister chromatid cohesion protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3144</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the SCC2/Nipped-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R8P8">
        <property type="interactions" value="255"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R8P8"/>
    <dbReference type="InParanoid" id="A0A1E5R8P8"/>
    <dbReference type="OrthoDB" id="3973167at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090694">
        <property type="term" value="C:Scc2-Scc4 cohesin loading complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061775">
        <property type="term" value="F:cohesin loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140588">
        <property type="term" value="P:chromatin looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034087">
        <property type="term" value="P:establishment of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071169">
        <property type="term" value="P:establishment of protein localization to chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026003">
        <property type="entry name" value="Cohesin_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024986">
        <property type="entry name" value="Nipped-B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033031">
        <property type="entry name" value="Scc2/Nipped-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704:SF18">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN DELANGIN SCC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12765">
        <property type="entry name" value="Cohesin_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12830">
        <property type="entry name" value="Nipped-B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Sister chromatid cohesion C-terminal" evidence="8">
        <location>
            <begin position="1268"/>
            <end position="1443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="9">
        <location>
            <position position="1240"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="9">
        <location>
            <position position="1502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ82903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1552" mass="177286" checksum="EDCC0111553A22D6" modified="2017-01-18" version="1">MVQEISSSSQQQEDQRVPNMEKAFPGQGSNLPNRLVDSLGCQPLNMLIPKSELXELINPNTNSASKISLKGLWNNKDLPFYSSIQDSYKSKEETSTVXINSLTDDSISGQLCDMLDDFKEEHFVFQNLHSNLQTAQNESSDQNLDTSLFEGLSKVAKDCIFNLVANDDIATGKTIKESTPEKFESSRKHYLKDEKENDDPVSTLKKVKITVKSLHSDSSGLEILPLESRDTKISNNFTALSETLDIIKQFKYEDSDSWIKVLDQNANETAYLTTPAFIDVIQMRVQNALNFKMNKSTFFQETQESRLVLSLENLQLLLNVCLANIESSPVTPLLQLGVKLKSINIILIILSSGLIKDQKRVVSPHYLDKSLSFLSMMMQEVFENIDYEVDFATLTILQSTVSLFLHSLTKGMPIDESLVAKILYSFIDIIINGNDFFKFNSENKFQACWDDIKTKTTQIYVYIFQNYSTQRDFIIDLFLPSFEKVPSSRSTKKLFKVGKSNPFYMSYFTYNVLSLFQSTKITVAQFNNSKNDEELVDEIISNIQNRSLDLSSWCEQITEGVLKKVFENYADLKNSFDIFCNDLIQCLVKPEWCAAEQLLTALTKKLLFVFSPTSQSNVQIEALVLAQLSHIGSAMLNIKKTEKNVGLSKDFSVAALCNYPERIENVMTNFNKVRNHLLHNDNENSSFYLWGVELNLLLNLRKLKNDGVSWHEKLTKYILDVANYRDFSTNNSKHAESEIESCYTAITQSCSLLTLYEPYLKLVLSLIDSNKIRLRSGAVKCFSALIAKDISMIDVPMIQETIAKKLKDSSSSVKDSILDIIEQSPKIEQFYKEVNINYNDDSVAVRKHVLKLNKRIYETSDSLTVKAYVLSYILKKIEDEEDAIIDSAKSFLQNELFFKIGILNNKPDKQESYCSDIIKIISAIITSSDKCNEMFYLFFNFSVLNERLFAADQYTALQGSVKVLTKVTIAEILALQQNDERILETKENELKKLLGLLCVVASSEGSFITREQLSTLYPFLTTTNDSNTRLSIFKIFNLTLIKSSVSLRPKFLLEMETFILKSLPKLSNREMEEAIQLAWLITKEKRDSQRVIKACFSCLKHLSHYIKIVAKNPAKVVCDGKLQRLLHMSTGFARYCEIANDNGEHSDQLKPNEKVYEYVTKCLLMFTKQKVDNSVRALAIKNLANLCSSRPKLFNSSHVLSVFDHVFESDNNQIKTIILNSFYSFFTREEQKILKKAGVDGTVSSDLSFKRKSSGSSEKHEKFNDGICSALVTRYLNQVLKLCLCKDPAVSTSAIKFLSMVDSYRYANPSHYIHCLIALLINQSTVCRKLAEKMIMDVLNNFEAMIFSGLVSGLMCAIQYNKLLQVPVDENFLNKVQTIVGTTKSNKARIATCITKCFQLCFENREDKAEPSYYGCLFLCYNLLKAQYEDRYSLYKLMKMIDVYIQKLDEQIENFEDCDPKEFDVDTKFNTIMEQNILLEFRTCMFKNYVLNEDTLFIVGTDEEKELKNTTVSLSASPLSFDFSSVCYHFDPNLQKENIIERFWTIRNSFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R8S6</accession>
    <name>A0A1E5R8S6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein BUR2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3187</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R8S6"/>
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        <property type="interactions" value="118"/>
    </dbReference>
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    <dbReference type="InParanoid" id="A0A1E5R8S6"/>
    <dbReference type="OrthoDB" id="25002at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="456"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="457"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="475" mass="54540" checksum="7F5D852919393F24" modified="2017-01-18" version="1">MSFYESTNNSGSSLSVSKSSRSVIIEDGTTLKYNDRVLWPQIIKEPANQWTYSTAQILDKLNNSGNVAATKKKIERSLLLFYKMKAALNLQEPTYIAACILFYRFWMTFDLPADMNDCMDLALSAMVTACKISENNRPIRAYVDAAVNILNSGLPMNQQKLEKIKWRVRDRFSANEMKFLSMMGFDFDICNPRHFIEEIFSVQYRLSRDKDFPKDFEAVFPSILKEARIFIYQTEAQPVSLLCDGMIYIQYSLVFAALQYKKKGHSDFKFPKKFFTKRFPNSFDPLMLSELANSHYKLEKNFLKMGSNKGSLLLMTADDIREITDEEVEEVHGADNTSQPHILEAQNKKGEASENGNSTIESKTKMNHEDANETDTISNYFSNDLETSQDDQNVTLDKSQKEALEPPRKKQCFENAKQSISDFYFLDVDEGVSEDFLKYTEEKIQKLYEEEFKRAALSNSSTPIPQVLNTTSKPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAV7</accession>
    <name>A0A1E5RAV7_9ASCO</name>
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        <submittedName>
            <fullName evidence="4">SWR1-complex protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2619</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
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    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAV7"/>
    <dbReference type="InParanoid" id="A0A1E5RAV7"/>
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    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140849">
        <property type="term" value="F:ATP-dependent H2AZ histone chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037651">
        <property type="entry name" value="Swc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058986">
        <property type="entry name" value="Swc3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057558">
        <property type="entry name" value="Swc3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28108">
        <property type="entry name" value="SWR1-COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28108:SF1">
        <property type="entry name" value="SWR1-COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24707">
        <property type="entry name" value="Swc3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26242">
        <property type="entry name" value="Swc3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SWR1-complex protein 3" evidence="2">
        <location>
            <begin position="110"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Swc3 C-terminal" evidence="3">
        <location>
            <begin position="854"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="468"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="300"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="390"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="409"/>
            <end position="421"/>
        </location>
    </feature>
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        <location>
            <begin position="489"/>
            <end position="506"/>
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    </feature>
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        <location>
            <begin position="525"/>
            <end position="542"/>
        </location>
    </feature>
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            <begin position="544"/>
            <end position="569"/>
        </location>
    </feature>
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        <location>
            <begin position="570"/>
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            <begin position="633"/>
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        <location>
            <begin position="715"/>
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        </location>
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            <begin position="769"/>
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        <location>
            <begin position="781"/>
            <end position="791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ84038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1029" mass="117079" checksum="33A1C2668A0EB8DF" modified="2017-01-18" version="1">MSAVTRTTRRSTRLKTPVQDQKPKEILRDETLGVKLENIEKTPASASNTSKRHTETSALKISTNKKRKTDGKRKTNSLKKRHNTGASDEESEDADGFESYAGSHVEHSSEFEMIEGLPCSSDLPPDNYQQFFTHPLSIIDSAVLYNALQQSRKTWCHGEMFKKYWSKSRDGSTYHIKLYRDDQDNLLKDSXLNESQSLLNNGLNTTNSTNNNGSMHKFTDCIMAAGPHQFKIKLFILKNDELEESWKEKQDEIKKEKEQLREQQKLLKLQEQEAKRQEQEKLKEMKEQEKEEKIKLKKLKQEQKEVEKHERELNKQKQKEERLKKKEEMKVFSLLKKQEDAVRKQKMXEERKRQKEEEKIKKREEKLKAAAKLREEMRSKAQLKKEKDSSTSATKTKSSTDSNKGSTSNASPQPRTMYTSPEEQKMIVNLNRMAKADPKLGTLMKKVAAAYASPEEITLFKIHIEEAKKMDPPPNWEPVDYSKINEATESEKPEQVTPKESKEENQKVNTQNKNXLVEQNKHQRPVTTKSEHLNNXTGNIASENGKDVRENQNSDESNKEITESKDKDTAVSSKTHSELPGNTNNYKTNEGIMNNKSNMDKPKDRDGDETEVGEATLKSGASNENASKEHCANSASLESQTKTASQSGAVSEANEKGGDVDKQDPKLESGSSPNQQVNELSKTNLENAKTDGNSARVSSTESNDAVALQVRNVDKTQSSDGATTLQKTDATSKTFMSADDMDKPNLSNVNPENCSDKLEKDASTSNGQELDKTTDEKKQVSISSNDSSNSSGFAQDSKGVQPLQNKILTSKANSVIADIPDQTKQNVRDTEKGASSTGGTFIVKTENTVQKEEEQLSLFQQKYAENADLLIEFNEHTSERFLFPKDCFIDYDFENNVYTISWMILYNAAEFSKYCGKRKIDYDYKNNQYTLFPLEVLEKGPIPLYSSFTMQLSDIPRKFRPIIINSCVQNSQAVKEKMETVIKRGQRLSGYNLWYQIDAYDNEDLGEELRSNIQAYEKSFKYKRASIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB22</accession>
    <name>A0A1E5RB22_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3063</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RB22"/>
    <dbReference type="OrthoDB" id="3971900at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="58"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="146"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="230"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="310"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="376"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="188"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
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        <location>
            <begin position="81"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="146"/>
            <end position="158"/>
        </location>
    </feature>
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        <location>
            <begin position="165"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="243"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="488" mass="54244" checksum="1B0FB114FC532A54" modified="2017-01-18" version="1">MDQLXKDTDPVKQPTDLEAETPSKVQQRAISETTGLYHLKLSSKNTDLADGEKSFKPAVSIXPSLSPKRSLLNVRPKSLMKKNSTXSGLKNGSGNRLNFYDENGAASNTESSSHSAREPSSNSSSRRASLVNASLLSSGVKTSSYGASHNLTQGNTPAHTGPSLHFTSENIHPSNSADKIMTKNEPTLEDMLLELAKKERRVVELENELTLAKKQLKEFELECSHKLHSNGMIPLSENRDNNNSKTQTSTNSENEQSAQANAXKLPMETDILSKWTNKLKETTDELLHIGGVAVGGXGSNEDQQVISNTTENDQAKRSDNNLTQAKNSSSLQPKPSLLQLGFDKFNNILGELYEEVEPEESTKSIVENQDGLDKLAXSNHKTKNQPTNVQESNKQKRGSFGENLDDQQISKSSPYYNRKSTFFDKLKNKINEFTLYEEDEEEYDRAKDKKLDKMIIVDDFQDCSIITTDQEEEEQGNLTQDSVLLKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB62</accession>
    <name>A0A1E5RB62_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">General amino-acid permease GAP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3095</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB62"/>
    <dbReference type="InParanoid" id="A0A1E5RB62"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000001">
        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF1">
        <property type="entry name" value="GENERAL AMINO-ACID PERMEASE GAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="111"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="197"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="220"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="364"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="412"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="442"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="9">
        <location>
            <begin position="82"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ84139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="525" mass="56855" checksum="672410DA4220D0CF" modified="2017-01-18" version="1">MSDLEKSDIVTIEHPQCSKKSSQTVNNYEYTEHESSGSRWQDFKDSFKRVDLETLDPNLTRAERAALATARAPLKKNLKNRHLQMIAIGGAIGTGLFVGSGASLRAGGPAGLLIGYGISSSFILAVICAVGELAVEFPVSGGFTTYTARFVDESFGFASNFNYMILWLIALPLEIVAASITVNYWGTPKKYRDGFVALFYVVIVSINMFGVKGFGEAEFVFSIIKVITIIGLIICSICLVCGVRGSYIGGKYWHHPYGAFVGDNAGARFKSTMSVFASSAFAFSGAELVGLAAAETENPRKALPKAAKQVFWRIALFYIVSLTLVGLLVSHKDPRLVGTSSXDAAASPFVIALVDHGIKGLPSVINVVICIAVLSVGNSCVYVTSRTLAALTDQGFLPKKLKFDYIDRNGRPLVGIIFTSVFGLLCFIGASDKEGEVFDWLYALAGLAALFNWASICLCHIRFRLALSSQGRSTEELSWKSPLGVWGSWYGXXHXYXCXXXXXLXXCXAXXLXXNXXXGXCXXLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB98</accession>
    <name>A0A1E5RB98_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GPN-loop GTPase</fullName>
            <ecNumber evidence="7">3.6.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2793</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Small GTPase required for proper nuclear import of RNA polymerase II (RNAPII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Binds to RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB98">
        <property type="interactions" value="909"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB98"/>
    <dbReference type="InParanoid" id="A0A1E5RB98"/>
    <dbReference type="OrthoDB" id="243313at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17870">
        <property type="entry name" value="GPN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000579">
        <property type="entry name" value="GPN-loop GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030230">
        <property type="entry name" value="Gpn1/Npa3/XAB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF8">
        <property type="entry name" value="GPN-LOOP GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="290"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="308"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ84166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="393" mass="44240" checksum="6761F3A524C50FC5" modified="2017-01-18" version="1">MHTVICIGMAGSGKTTFMQRLNSHLHAKKEAPYVINLDPAVLKVPYGTHIDIRDSIKYKKVMQNYGLGPNGAIVTSLNLFSTKIDQVLNIVEKRSDKFKNCIIDTPGQIECFVWSASGSIITESFASTFPTVIAYIVDTPRNTSPSTFMSNMLYACSILYKTKLPMIVVFNKTDVQKADFAVEWMKDFEAFQTALMNDKELNGETGMGSGYMGSLVNSMSLMLEEFYSQLDCVQCSSYNGQGFDDFMNAVDNKVEEYESYYKAERERILKEKAEDMKKQKDKSLNNLMKDLGLEDKQKQNNGKKKSGDEDDGVDVLSDYEEGENDGVXPRDDEEDEEAVQREYTFSGEDREAGEINSNNEPDLQARYQEAFEQTAKEASSATAENIARYIKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBJ8</accession>
    <name>A0A1E5RBJ8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleoporin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2516</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBJ8">
        <property type="interactions" value="156"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBJ8"/>
    <dbReference type="InParanoid" id="A0A1E5RBJ8"/>
    <dbReference type="OrthoDB" id="103454at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1380">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="986"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="986"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="998"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="2">
        <location>
            <position position="434"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1212" mass="138494" checksum="803673CC2C2C1C9D" modified="2017-01-18" version="1">MSLSFKPRKELLEDGITQPLEPSFCSANSMDFAGVPFEQQNNSIKLNTLVLTENEKYAVSQVSAPLAFXNPLTNEQSNIQTCTLNPKSGFALVLTKDGFMHIWSYLNKLPLASSNVLSHNFSELLPRSTSTTSLADFEFEFVANDIKLTPGLLIFNKKTGETFYYEDIQCDLSVEYAHQYKITLKANEHIVQHEYIAGAGLMVVTDVHHNRLLLISIKDSLGKPLTACKEQLLNDSSXGWKSFLPKIKSAEIRCLNQEDFLNKTDKSITTLDADGGLVNWVVNRDSHFKNLETNIYGAVVNESLESFYPYTFNGTKFCAFQKITENVYLFVSKVQAEIETLQRPKEHIQYHYIVSVVKGVFQSNTNTNEQMEVLSTYRIKTVVDDLDRVNVFANYSSNNTFFPVFVTFKNSIVILQLSTKFDSFFGFQDKWEDLVSFKPDSENFACTYDKGKLFFFNTYVNQLLKITLKEFNNTGQKKNKKSLHLNSFIKTHIEQSIFFNTTGTANPVEFDLPKHLELKKTDIEHDLTAVVDEVWNSXSSYLPAYPAKTTDPNLVKQYLQCKLNVFERLLSYIXQNFNEVIDANLKLSILYKTEIIDCSFVFYDNLTSSAKSSLVTSANSSSLFQLESNLDHFPEKYENFIRNWNGSIASTPSDVNXQEQFVNLVITTLYETVFEKCESHYKYKILNLSSSKESSESLPWFSSQTLMEAINDLVLQYSSLAISKTEQSNENLLKLIKILYYQASQYETLSKCKVFEQNNLKWCDLVIGTHDDSYYRSLILMLDFYNDLKGLVYTFDKLGEEMIPDSVYSDYLKKYGTAFTAEICDYFTKGTGNXSKLFRIFKSNTDPVVKSMLTQYLNGNRALYGNVVWMDDIINENYLGASNALYKLSKDSSKPPKFQAKQFELSLSKLALLYASQDKAIGELAPNVNEQLLKIQTGLYELKAQKELFDKLQISGKTIEELSELDQNSEMYKLAQTLVENLQKTSIGSTDKQAPKTSSDKLEVNGAQSKEKKPVSKKDTKALPKPVQTIESSVTFPVQQLIYLFTVLTKSNTLFEDGXFCALESLSLSISEMEYETFQFCLGFIWRFILYNIDEKKEKKNLVKLTLDKYFEHELYRHIPLPLTTDVYDQTSFRSAECVHEFLQNTHSAVTLHINTQKFIQENVDLLSKVQNDAKIQEFITSELPILINESNAKYGNQVIVNFSNGKLSFIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBS9</accession>
    <name>A0A1E5RBS9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">V-type proton ATPase subunit F</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2722</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d, e, f and VOA1).</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Vacuole membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the V-ATPase F subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBS9"/>
    <dbReference type="FunCoup" id="A0A1E5RBS9">
        <property type="interactions" value="717"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBS9"/>
    <dbReference type="InParanoid" id="A0A1E5RBS9"/>
    <dbReference type="OrthoDB" id="10261947at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033180">
        <property type="term" value="C:proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10580">
        <property type="entry name" value="ATPase, V1 complex, subunit F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008218">
        <property type="entry name" value="ATPase_V1-cplx_f_g_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005772">
        <property type="entry name" value="ATPase_V1-cplx_fsu_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036906">
        <property type="entry name" value="ATPase_V1_fsu_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01101">
        <property type="entry name" value="V_ATP_synt_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13861:SF2">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13861">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01990">
        <property type="entry name" value="ATP-synt_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015945">
        <property type="entry name" value="ATPase_V1_F_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159468">
        <property type="entry name" value="AtpF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="5 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR015945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ84033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="150" mass="16545" checksum="90E6121E74B2B0D4" modified="2017-01-18" version="1">MSATKSAGSAEQKRNLIAVIADEDTTTGLLLAGVGQITPAKESVESEEGLHNNNSTDSKVVSSNLGKNFFVYKDGKTTRQEIDDIFHLFTEQRKDIALLLINQHVADLIRSSVDNYTAAFPAILEIPSKDHPYNPEKDSVLKRVRKLFGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBU7</accession>
    <name>A0A1E5RBU7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">COX assembly mitochondrial protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2985</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for mitochondrial cytochrome c oxidase (COX) assembly and respiration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the CMC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBU7"/>
    <dbReference type="FunCoup" id="A0A1E5RBU7">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBU7"/>
    <dbReference type="InParanoid" id="A0A1E5RBU7"/>
    <dbReference type="OrthoDB" id="532630at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013892">
        <property type="entry name" value="Cyt_c_biogenesis_Cmc1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08583">
        <property type="entry name" value="Cmc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184707" description="COX assembly mitochondrial protein" evidence="5">
        <location>
            <begin position="23"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU364104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ84370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="117" mass="13740" checksum="58B2EDF0A394225E" modified="2017-01-18" version="1">MRLTFLFIISTFPPACYDLIQALNECHQKEYYKRALGLCNVEKDALSKCLHDARLEGTKYAINKNKEKRKRLEEKWKKMQEEQYGEDMFLKKLLQKKIAERDGKVAAEQGLANKTQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RCL2</accession>
    <name>A0A1E5RCL2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitinase</fullName>
            <ecNumber evidence="2">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2691</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RCL2">
        <property type="interactions" value="599"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RCL2"/>
    <dbReference type="InParanoid" id="A0A1E5RCL2"/>
    <dbReference type="OrthoDB" id="76388at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06548">
        <property type="entry name" value="GH18_chitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF317">
        <property type="entry name" value="CHITINASE 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54556">
        <property type="entry name" value="Chitinase insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="6 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH18" evidence="10">
        <location>
            <begin position="37"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="11">
        <location>
            <position position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ84323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="508" mass="58478" checksum="86A3B423D4DCE687" modified="2017-01-18" version="1">MLPNPNTVPYSNYGITQKSGLKELDAANKQVSGDHEMVSAVYYSNWSPYSPRNFYPHDIDFSRVSHVYYAFFLIDEKTGSLKSSDEYADYQLNFVKKLYHEFPNIPFLNDPKKHSLEDKTKATGMLGELFFLRTSKFLNSISQGKNSDISNFKVCMSVGGWSNKEAFHAIVKDEKKLARFVESCVETMFKNGFDGIDIDWEFPEDDGFEPQKYLEMVKXIRSKLDELELDLNSTDSDTGKVHFQLSLAIPCAKDQLEYVPLQKIDEYVDYYNLMAYDLAGEWSGKTNYQSNLFPIASTSPINKXXHLSSLRKVVHHFKXKXHDSNXSLNIDENVKFLLHTVKLPSRKIILGMPLYGRGFTNVKIXKDASEKPRLVDRDYKGVGGKSPDEXGMWLYNQLPDGTEYFDKTAVSAYSFNTKTNTFVGYDNRESMKHKALYVLTNKLGGAFWWEACGENRKMPLVKEFHDNLNVTWAKXEKSIWKTPKLRAYYLKKFGHDTTMLKSIFXDNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RCQ5</accession>
    <name>A0A1E5RCQ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Histone acetyltransferase</fullName>
            <ecNumber evidence="2 8">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2974</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the MYST (SAS/MOZ) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="2 8"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RCQ5"/>
    <dbReference type="InParanoid" id="A0A1E5RCQ5"/>
    <dbReference type="OrthoDB" id="787137at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990467">
        <property type="term" value="C:NuA3a histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.60">
        <property type="entry name" value="N-acetyl transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002717">
        <property type="entry name" value="HAT_MYST-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050603">
        <property type="entry name" value="MYST_HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615:SF161">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE KAT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01853">
        <property type="entry name" value="MOZ_SAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51726">
        <property type="entry name" value="MYST_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYST-type HAT" evidence="10">
        <location>
            <begin position="208"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="118"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="619"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="694"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="119"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="134"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="147"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602717-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ84363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="714" mass="83095" checksum="031B41327CFC32AB" modified="2017-01-18" version="1">MPKIGKEADSTHLVRLKYDPKKVYRYERKLHFDFPKFCEKVESSDQALKENDVEMIPKKHKPKAKTKQQQQQQQQQQQINNITLDTQVPSLYDIIVRTIGFDQTLSINPSEKPLEDISNVKNSAANQKPSQSYKKGKRSKVQKSKSDTGASTKYTPNTDYTIDDMKPNDIDYTVFQELLNLCQKTDLNAIDRHSNFLSSTKDSENAPTKQPLSFKVWLQKSLVQPWYLSPYPLELERKKMATSXSSQTPAYXDILYICEYCFHYTLDKYKHHRHSTKCRKPVGGECIYSDPINDIQVYEMDGLDDVNIARNLCLFSKLFLNSKILYYDVEPFLFYVFYHKNEFFGYFSKEKVSTNNLSCILTNPNLHNKQWGQYIIHFSYLLSSIAGSYGSPEKPLSDLGLLSYRKYWKFQVLRALITLATSLENKVQHXNFPNTSFLSVSLHDLSVLTGMIATDVVFGLEQLHVLHQNAGKEYKIEINVNQWKRDKKYLLWLNNIXKIDGVKYLDWKPPLFGPSGGINTQVVNKWTQSNEPWIKEYLYQYLHETGNCHSLYAQRRLLIDKILSRSTGDXQNTTTSNDWELCHVEYIREKPEKLSTLQQSDSLAEEVEQILTEPIAEVYNDELNDESFGEDDFSDAEDEDERGNDDVGGEDEDEYFEPETDDEIVDFETFERESEKQKRYFFRKNVTTAKRLIPMPNSARSLRTRAKPVLKSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDK4</accession>
    <name>A0A1E5RDK4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Heat shock protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2309</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDK4">
        <property type="interactions" value="560"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDK4"/>
    <dbReference type="InParanoid" id="A0A1E5RDK4"/>
    <dbReference type="OrthoDB" id="47330at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070370">
        <property type="term" value="P:cellular heat acclimation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19499">
        <property type="entry name" value="RecA-like_ClpB_Hsp104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000120">
        <property type="entry name" value="ATP-dependent chaperone ClpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000025">
        <property type="entry name" value="ATP-dependent Clp protease subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000010">
        <property type="entry name" value="Chaperone clpB 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1780.10">
        <property type="entry name" value="Clp, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036628">
        <property type="entry name" value="Clp_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004176">
        <property type="entry name" value="Clp_R_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050130">
        <property type="entry name" value="ClpA_ClpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638:SF18">
        <property type="entry name" value="HEAT SHOCK PROTEIN 104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02861">
        <property type="entry name" value="Clp_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81923">
        <property type="entry name" value="Double Clp-N motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51903">
        <property type="entry name" value="CLP_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="Clp R" evidence="10">
        <location>
            <begin position="4"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="437"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="478"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="437"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ84988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="902" mass="100871" checksum="8D7E702AA6CE7A96" modified="2017-01-18" version="1">MNDSSQFTDRALNIITIAQKLAQDHQHPQLTPVHFLAAFVETPVDGTKPYVQNIIENARYDYXLFRKTVNKHLVRIPAQHPAPQEISASYGTAEVMKEAAKLQKQQKDSFIAQDHLFMALLSNSQXKDIFKECSMDIEAVKQQALLLRGNSRIDSRSSDTSTPLEYLTKYAIDMTEQARLGKLDPVIGREDEIRSTIRVLARRIKSNPCLIGEPGIGKTAIIEGVAQRIIDEDVPSILKNSKLFSLDLAALTAGAKYKGDFEERLKGVIKEIEENSEKQLIILFIDEIHMLMGNGKDDAANILKPALSRGGLKVIGATTNNEYRAIIEKDGAFERRFQTINVAEPTVRQTVAILRGLQPKYEIHHGVRILDSALVTAAQLAKRYLPYRRLPDSALDLVDISCAGVAVARDSKPEELDSLERQLQLIQVEIKALERDVEADPTTKERLQQARQKEASLEESLGPIRKRYEDERAGHMELTSAKMKLDEMENKAADAERRHDTATAADLRYFAIPDIKNRIEELEKQVAEEEMMAGSDTLVQNVVDSNTIAENAARLTGIPVTKLSESENEKLIHMERDLQSEVVGQSEAVKAVSNAVRLSRSGLANPRQPASFMFLGTSGSGKTELAKKLAGFLFNDEGAIIRIDCSELGEKHSGSKLLGTTAGYIGFEEGGLLTNQLKRKPYSVILFDEVEKAHPDVLTILLQMLDDGRITSGDGKTIDCSNSIVIMTSNLGAHYINEQPGSKVNEATKELVMSAVRLHFRPEFLNRISSIVVFNKLSRKAINKIVSIRIKELEERFAENDKHYHLKLDDAAMHFLTENGYSPDMGARPLNRLIQNEILNKLAIRILKGQIQDKEDVQIVFNKEKDGLDVIANHQGKALDDMDIDEDYEDIDIDDLQQPDYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDP9</accession>
    <name>A0A1E5RDP9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alpha-agglutinin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1783</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDP9"/>
    <dbReference type="InParanoid" id="A0A1E5RDP9"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024672">
        <property type="entry name" value="Agglutinin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033504">
        <property type="entry name" value="ALS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011252">
        <property type="entry name" value="Fibrogen-bd_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793:SF2">
        <property type="entry name" value="AGGLUTININ-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793">
        <property type="entry name" value="ALPHA-AGGLUTININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11766">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01056">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184702" evidence="2">
        <location>
            <begin position="21"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="domain" description="Agglutinin-like protein N-terminal" evidence="3">
        <location>
            <begin position="53"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="803" mass="86484" checksum="A7AE5A6CE07495C5" modified="2017-01-18" version="1">MRFLLLNFSLALSSLMLTTAAEYISDISFSNLEFSVLDGSSAYPQNVWKANLDFSIPNPSAIKTDDYFSIYMPKVYQVYYNYPDHYFNISMNGNAVFQCYAEQQAGSKNTESIMSCSSYTDFTQTAEIFGSLEFAFTFDCGAGDILSSNEIVNANYFQSGVSTEFEFYDDNDNSFAAQVTFAAMSVSDDLMNVVRNPADETLEIYYAAPRCSSNGHITSGTVQYFMQAGTTFDQLHSNGGVSSDSTHNEFYMLNATQNNMFAPKKMSTENGQNVAKFGFSSVEPDEMIWFNVIQTVGPKTAAVSNTYEATYTCVSGSSTWSEVHNTLTVIDFEFASNYGSLSYTVPTVTTKTTTFTGYWDSTYSTTTTEAINTTPVDNTVIVTIHVELPTPVVTSVSTFNNVQSTVSISTIKETDMVTELNTKTQLETQTQTLTTVSTTNKLITTTATTSVEVPTTVTTSKVQTTVVSSLIRETETVTTENTIVSTLTFPVVTQSVTTRVTNFTTEYYTDIITVPSVFVSTFVDTQLSTVTTEYTISITSLSTATATTVVPTAMXSSYVSTFTDGXTFTSLVTSAVLSNVTLTSTATTTTMPSGDIVTETQKTTMTTLVGPVTVVESVTVGASETDTQAIFNSATTVTTTVPTSLTXLPTSSSNSGDNQQNYKLKTASLGSLESVSTEIAPVSPSSSGTDFPTSIFTETVTPTEPSAVSTIFDVSSISTVETFSKKVGTYISTAKASISTISSFKSASTISPISFLTSCSSTDSSPASTSTYSAPFIQFENNAKTIQTPLALLFSILFSIICL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDR7</accession>
    <name>A0A1E5RDR7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">tRNA-aminoacylation cofactor ARC1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2040</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDR7">
        <property type="interactions" value="347"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDR7"/>
    <dbReference type="InParanoid" id="A0A1E5RDR7"/>
    <dbReference type="OrthoDB" id="19141at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017102">
        <property type="term" value="C:methionyl glutamyl tRNA synthetase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02799">
        <property type="entry name" value="tRNA_bind_EMAP-II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000199">
        <property type="entry name" value="tRNA-aminoacylation cofactor ARC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053836">
        <property type="entry name" value="Arc1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051270">
        <property type="entry name" value="Tyrosine-tRNA_ligase_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586:SF33">
        <property type="entry name" value="AMINOACYL TRNA SYNTHASE COMPLEX-INTERACTING MULTIFUNCTIONAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586">
        <property type="entry name" value="TRNA-AMINOACYLATION COFACTOR ARC1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21972">
        <property type="entry name" value="Arc1p_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="5">
        <location>
            <begin position="201"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="134"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ85026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="368" mass="40989" checksum="0EC99553D87D5EE9" modified="2017-01-18" version="1">MADLVSKFSALDXKQPTDLSNEQKAIKAQWESVVQSGSITEHLQDLNLQLRDQTFILPGTIELEEVDVKTLEVVLPILKDLLASSKDITNNVATYRHIVRWALFILSKLNVDDEILGGMNLNLELPKEIIEKKKKEEGKDKAAPASAETKKETXKDAQQPRGKPDEETLKKLREEAQAKKAAKKAAKAQAQPQQQEAVKPAVSAIDFRVGFIQKCVPHENADSLYVSTIDCGDKEGPRTVCSGLVKYFPLEAMQERYVVVVCNLKPVNMRGVKSSAMVLCGSNDDKIEFVQPPEGSKPGDKVFFEGYSDQEPLNQLNPKKKIWEAFQPNFTTNENLEVIFKDEDGVKKLTSKLGQSFKVASIVNANVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDW4</accession>
    <name>A0A1E5RDW4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein STU1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2141</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CLASP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDW4">
        <property type="interactions" value="157"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDW4"/>
    <dbReference type="InParanoid" id="A0A1E5RDW4"/>
    <dbReference type="OrthoDB" id="46159at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060172">
        <property type="term" value="P:astral microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024395">
        <property type="entry name" value="CLASP_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567">
        <property type="entry name" value="CLASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567:SF9">
        <property type="entry name" value="CLIP-ASSOCIATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12348">
        <property type="entry name" value="CLASP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CLASP N-terminal" evidence="8">
        <location>
            <begin position="384"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="657"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1034"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1160"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1230"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1276"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="660"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="702"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1186"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1236"/>
            <end position="1248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1279"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="9">
        <location>
            <position position="1293"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="9">
        <location>
            <position position="1384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1533" mass="175969" checksum="8785247859D33B13" modified="2017-01-18" version="1">MDKTFSKNSFNGEISYQNGKDLESKRVYPLYQVFTDASVSVDEKLQLVTAFKKHVKLKKIDENEFQSYFDAFLFYLDDXKNDTKRLEIITKDHQSKLFPQFEQQLYSTFCHLIARASWQQSINITKDLIKNSMLVFIQNENKNFEMANTKRAIEGMFLAEHELFDNILLNDFLLNKQISNVLKFDILETIKTVILPLTTKDLMLDENDSEILKKHQQYLFKYNFFLQKQLNKDNLDKLRNCLDXKQDNEKLTQWCLEICENYNIPYLNQTKTPAPTNHINTFTPENFNEXTSKENSILISQHEIDKSNLQSEKDKENIKPMFDTLETELQRIISSKSNNMFPSLISSSTASASSQPHYTSSFSTTISAKTTTRNFTSLEYFFKELDSSLSAFHLKENESNWKKRQSFILKLRSFLSSATTILNENTHAFVKHFKEMRYIDCIHKAVHSLRTTLSSSACMLIKELCSIFKKNLDLFQEQFFEILKPLLSSTKKLSVNNAHMAILSMLSHCSFNKKVFLASANLSRDKNVQPKICSCDYLRLFIIKSANSFQPSTFHINDETTENSDEFAYQLKSWVKNLITTPQTLVRSYCKIAFWYFYXLHPKMATQLLTDLNPQFKKSVENDIPPYLNIQYPMPSSDIKPNQRESSVSRFKKFKALSQPPAQSAYTTNSPSRREDYNAVKHSLSRNTLRAHSDSSIRKTPKSNNANSSTLEGQNDAMPSTRKTLSREIDIPNTTTKPSLQPASPFVSHTPKTTLYQHHTPKSVLNSYSKKVQEGSSQTTDVSTEDLINVLIEKLQAVSSNSFSNVEQTLDEVVVYLSSXNVDENIKKVKHSELISALRQIALHAPMAFRFVIGKKSFIKLYAENVQYLSHLIELFAINNEFSIKHLLDSLSASIVFHSILTQIFSSEMDTGCQSSLFYVKYRKDILNYGLRSCIKLIELDANKSGENVLASNXEESLSHVIKQWGNYFNEEVYFELLTRFQINNPTAFKDYVLKLPVFQKAKIAXEVQKRNAEFDAYSILGQHLRKTVEDDLXSPENHLESPPLKRKRSLSSSVMDTYEKRFVSNVTDPNADDVLMIDDQTVEKKAMELTMVHPWKXLLAMSKQSSDEPDKRISSTGSVVLHKMQVASAVESEFTQCYGNDTMRLSEMTKIIPAFSTXNXNSLDSKELSKNGKGKVTNLEGKEKEEEEKEEEEKEEEEDVIDLSDLFYGSGAETLSPLKFDIDNFHKEQEPAKQEMQSLSKTQTESESLTDKENLEKISFENDAVTQITDLPLKESASGVQTQDXXQKAQNEAKQPETDETMEEATDLKDEQSAKIVEKKVGPKHNEEMKIEXVRDLKSLNKFLGEKNQFEKEIFPTNXALSQMFSAFXVKLIECFKEASYADFAEYAKYTSIMTDWLSKADLQKXALEFLPEIVSTFQVQDYVFDDKHIITMVDTVASLCQTTESYEKLYFSDSCESETLFVMACMQQIVASKNVDIISPSILNNLTDEKLCSTNSGIRVQTYRVIGRLLDDLDYKMSLQPCTLELIKRFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE72</accession>
    <name>A0A1E5RE72_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Single-strand annealing weakened protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2230</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RE72"/>
    <dbReference type="STRING" id="56408.A0A1E5RE72"/>
    <dbReference type="InParanoid" id="A0A1E5RE72"/>
    <dbReference type="OrthoDB" id="4034365at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070336">
        <property type="term" value="F:flap-structured DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000736">
        <property type="term" value="P:double-strand break repair via single-strand annealing, removal of nonhomologous ends"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021624">
        <property type="entry name" value="Saw1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11561">
        <property type="entry name" value="Saw1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="685"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="387"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="475"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="965"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1023"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="232"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="487"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="966"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="990"/>
            <end position="1002"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1112" mass="126084" checksum="ACC25717D9F7055D" modified="2017-01-18" version="1">MIMGDNLSAENNDTKENTGYKRDSYNHRSDHLDTGFSVPNTSNIYKKNASNALKPPIVFNNTSGNIPSLKNDDGHNFGTYKPVVNRDYSNSSKSFSIHQNSNTDKNNSTAPKSYRAHEMENGGFGNPKNENTSASRKNLLDLLGSKPNTDKVRQDVHLNPLSANSNFLNHDGAIRRGSTSSNSYKRNTDYYAPVSNLARLKNLTSTSKGTKFSETVFSHNDLGLVPESMGATSRSDSNSTNNRVATPVPPVSTPVNESNIKPALKKSFSGGISTQKKYNMNSPPNFHKRNMSNTFSAAGSQMSLPHAHPHLHSSNHPNSSLAEESTGNFSQLGHSNISQAPLSFIPNQREDSSKLVQQFYHDDSVYNHMDIFGSNHSDSSLKRIISHTANNDPSNTRSTTLNPAFGNSSRSMSNNQCDFEDEEEFKMIKQSKYLMDVYRDDLLIPFVSQTLASLNSIYVQKHENHTKHYLLRKEKEEPSNHRHHSRSNTVAANNPNGAISYDYTNTTALNEKEKTKQNDSKKLNTHSDKNGKYSVLVECSVDDELYKFNNVVLKKLSASLRSERLHLSKFHDLFQVLHIEFERIVDDLQVLHKNLVSGVRELESLNHNNIAYYENTMLRKIVSNNEKLYYFKIKTTKNLEILKSLYAKIQTLEKFNKLFIEIDNFYNDMGLFQRIKYLMKPYNKYFTSLGFLVDLAVFVVILLYYYRRAINAIKLLVLLIEKSKFETQTPCKTQSFNKEIHLHTTMPPSIAYVKLNKQTSLTLRVFVNRKEVLNNFKKTTTSANNDEFDMNSLNDMQKSVFEAPVLSSNSIIRLKKPLVKLFLSNKDLNDLVENIRNDLILILYEFSDELVVSQILNKMKIDSSIELFKVTQFVVSKNNLKIKDQNVNIDGLPVHGKNIKKVGKHKYIIDFDDKWGCDIVISDIRLLRNFKRIMDLNRYTLMSNTGSANPAGNKISHDIADFVGQQPLQPDTQGIDQFGESGKTQHLDADNDEADDDEENDSANEYSYIQGTSSSSNLILRSLKRGKPKKNSEENTTPLILQEEEHDGEGSNGLSAFDKEVETLQKQNADATSFGDQDLDVKFEASYSYKPISNLGKCIDIYVLKRPKHRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REC7</accession>
    <name>A0A1E5REC7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein LDB19</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2235</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REC7">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REC7"/>
    <dbReference type="InParanoid" id="A0A1E5REC7"/>
    <dbReference type="OrthoDB" id="3832628at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024391">
        <property type="entry name" value="LDB19_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13002">
        <property type="entry name" value="LDB19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LDB19 N-terminal" evidence="2">
        <location>
            <begin position="177"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="392"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="428"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="610"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="645"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="396"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="432"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="645"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="842" mass="94237" checksum="65078A85C404610E" modified="2017-01-18" version="1">MVFSRLGFKSSSSDKKTSSLSNKNKHKNDSNDFYNTKNLNLKKKLKYDPRGPVELAISIESPPCVMYGTTSNSTGALLSGLLKVIIKPLPQGQEPSQHQRQQLVHLTRVKMKFVQRLTYANPFVPSDNDVLNNCPDCLTKEVELASWDIIKEKLMNKGTSKNSAPELVTGNAEQRELEQQENGVPILPGEHSFPFSRLLSGRLPPSSKYGSNADSQLSYELIAQLQYFVPSSINKHVGKNNKKTIDLTLPIQISHSILRGHDKNSVRVFPPTDVTATCTLPNVVYPKSKFPLELRLEGVATENKRWRMRSLTWRLEEKIKIRQHSCDPHKKTLKKYEDKIREDQKNGKVCTNGNSSKQKNLGQRRPLKRNALTGPVVTTSVIDEGHEQILLGPVRSITNEETGTGENSQSGNDNDNDTITNNIDQTLENATSSNGSSSFSSSSAPPTNLLNTRSSSFFNTLSSNNEDYNGTVHPNDYVLRQQLINQQRLLREQQIEQERKKDISLYTEEVRTIAHDTIKNGWKSDFQNXGKIELITDIDCMHLNSGITNSKRNISSKNFMKNQHFLNFLKANKSNIACDVEDPVLGIYISHILVVEIVVAEEALQYANGTPLHPKSSASSSHSSHSKKAVSSNPNVMLTREELHHHHEPTRQHDITPTHSSSSSSSNSYKPQLISVPTGAARVLRMQFRITFTERSGIGIPWDDEVPPTYKDIKYLSPPSYDVAMSPVMSNEITFNHPINNLNSVISSSYLDNLNNRLESLTTKESNDKDDENSTDDEDQDTITIRANEMNPPPLAHFNLGLNNVQSIDGNVVGANDMMLSPRNTNVLNHPNNISNVFNSTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REF5</accession>
    <name>A0A1E5REF5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Spindle assembly checkpoint component MAD1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Mitotic arrest deficient protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1926</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REF5">
        <property type="interactions" value="334"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REF5"/>
    <dbReference type="InParanoid" id="A0A1E5REF5"/>
    <dbReference type="OrthoDB" id="331602at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.457.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008672">
        <property type="entry name" value="Mad1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23168:SF0">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23168">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD1 MITOTIC ARREST DEFICIENT-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05557">
        <property type="entry name" value="MAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="426"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="63"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="126"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="552"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="8"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="664" mass="77238" checksum="46F330B91EBE2553" modified="2017-01-18" version="1">MTRYVKDGSSSPFLENPNQSFGSNEAPGNNADELNAEILYLKYQVTRITHELDIKEGKHAIEVNTLTKKYQEKVSELDNLLNAFKTVQQDNDALSKKLSSLDXQTKENKGLEQRHQNTVVLLESKNSDLQKQIFDINAAHEQLELKNKEQKVELESLEDLLKNRDTELQQKLEEQNILKLENSKLEMLVANCKANENSGKTNKNIQSIANSNPQITELGYLKKELQTQLQYSRELESENLKQSLELKSVKQKKENAXFLTNENDMLRSKLAKYGDDLKQQLEQXHSENDSLKSELLNNEVKLKYDALIKEVALLKEQHGDWKEQRVFLERKCSELQIENEQLSNMKVDFENTINNLQKLNQEVEQQKQLAFEESKLLRQELDDFLASSSLKKEQGNSQGETNWNDMLDKYKNKSEDLTQELKRVNEEMKNSITNSSKKRKLEESGKVEKETQQAKDILALEHHISTLENKIITLESERSVLHDELQDLRNTKELKIRILQLRDSPLNKDQFVKKKMLQLLKKENEQLLSISLSDHSIEKFPKSVYHRLVMEQDNLKLELHESKKQMKRLKEMFNKKSAEFVESVNQILGYKLEFLPGSKVKLISCYEPTKSLEIDLINNTLKSSLSKHLPDWDRLLQQWVLERKEIPALLATIQLQLHALTNQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REJ7</accession>
    <name>A0A1E5REJ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KH domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2302</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REJ7">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REJ7"/>
    <dbReference type="InParanoid" id="A0A1E5REJ7"/>
    <dbReference type="OrthoDB" id="271862at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="2">
        <location>
            <begin position="510"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="801" mass="92245" checksum="B4E89455D86E7C7A" modified="2017-01-18" version="1">MQYYKHAFDVYFEPXFTEGELNPILRDXVTWKSTKDIPEALGSVLNKDGFTAPKFLVGNSDTLSSLILRSPSLEPVIKTENFARYYVTTNLKVTDPFETFEIAIXAETLESMKKDNYGKLDRLCDSLQLEYLITESTELYPKLDSRLLKNNSXSXPSTSGHYVMKFFGLXSQLLIAQPCLNSLCDVYKGFATHCLELCSFYDTALFGGPLYADITKISEFYNVEIYLPELKTSFVGNAQNKPLSKKLFVTSSNKENSLVVVDLLEKRLEALAKQKKYVMAIQSTMQGISGGKLFFIQKYYSTAIEKLMIKFGCFIDVQSQSNSVIFYSCNSVLLKTAVREFSNNILSKVYEFTFQCQDTSQDQAXVEFFMFQNVDIDVYHNSEKHEFQIIGAFFEPLLQKMVQTPLFDQMHIRNIRFLFELLPEFQDFITGKKFGKLNKIEEESNARVSLLFEQTTLVPLXKTKNDDXKHNGEQADETLFNITGTIYVEVQDESSLDTVIKGVHLMQMEVPFEKSIFIPESFHKAIIGNGGKLIQSIMRRHNVFIQFSNTHCYPQSXFSFIRFDNVLIRCPYKNRKTISDAENELFELETKILQNMEIPMSIDRRAEFNEXRDSAQSIKRDNKSLVLWKFDLCELESMMMVMNKGQRGIPKIQDXIGKLEEKNDVYIQFPEYDEYSFATEATWAASKQNDTDDEPDKILADEKVGVIIKGKNVGQDVLEKLKDDQLAXNYLPKKCHFKLGAVEXLSESKFQSWKSLLFYEYGCNIKYLKDSNSLTIYYYDAKNLEFAKAYIQQDXSVSLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REK5</accession>
    <name>A0A1E5REK5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATPase expression protein 2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1675</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REK5">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5REK5"/>
    <dbReference type="OrthoDB" id="4062665at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ85349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="565" mass="65734" checksum="DF9EAC3561DF837C" modified="2017-01-18" version="1">MLKRSFYVSQRVYSSAFSLEARVASATSSSGGSSAVNASFELKEDQIPTAQYIHSQRTDASENNTPKXLKHYIDLFRSGVTDIDLKSTFQTGKKAETIAQRQLLKPLYHMTHRYYAHKLEKLREAEYVCHSMDECNFLNREIVPGVGLHEIPKLVEYCVQSDAKIKNVMFFWKLLVGDNVAKYAYPNKEFVSPSTDLYLEDMLKICEKLFQMKAFVQSMEAFKYIFENYLGGEYVVNARFLTMYLKIRTGTYIEYSWKELNPLEHNFKKYALNQTFLMQDVSQVIEYIQDQVLDENGPWSLERNSELESNIIYFIAMHQPEALGSYIDHVYMNGAGSXSSFTGQVDTKILLSVVNSYYHLQDLGKGLQIMNKLIEHXGGYDKMDFNVKFWNQLFLIGQKAAKHXLDPDMDICINVWKLLKQKIDTNPVHETSCLSCIPFYQLLKDPKTKHPLRFQLLLDIFKTFINPCQLKLNIDTKLRVDERHLMKSILKTLINMKLYKNSTKCLEMIDAYNLGHKEELLECFYKARARDSEKRKSQRQREATRSKLLREDVDMEDKMLGGGLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REL5</accession>
    <name>A0A1E5REL5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C-CAP/cofactor C-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1848</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5REL5"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017901">
        <property type="entry name" value="C-CAP_CF_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016098">
        <property type="entry name" value="CAP/MinC_C"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51329">
        <property type="entry name" value="C_CAP_COFACTOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-CAP/cofactor C-like" evidence="1">
        <location>
            <begin position="107"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="260" mass="30091" checksum="59ACE7798CC5F219" modified="2017-01-18" version="1">MQXNLKALLHFKRTGFDECLTKNDHLLIESTLENFQKQRKDFQSNGIVDPEERLLLQEYERQLEIVQKRYQQILISNKNSLLSDLKLKENHTFSQKKKENTFFAELTTQSNKKVGQTIQLLDGKRVSHCVDCSILTNPHVPLQKPINVFDCRNCKIRIEGVQKYIGSVYLNEIKDSEICLSWDPSLKNQIRMSNLKNCQVRISKGKLSHSSERFVVILEGCEKCVFDSXSQDIVTIKDFDNIMGSEKSYVFSELSNLTAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REN0</accession>
    <name>A0A1E5REN0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Heat shock protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2113</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal/bacterial/fungal opsin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REN0"/>
    <dbReference type="FunCoup" id="A0A1E5REN0">
        <property type="interactions" value="63"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5REN0"/>
    <dbReference type="OrthoDB" id="536545at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15239">
        <property type="entry name" value="7tm_YRO2_fungal-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001425">
        <property type="entry name" value="Arc/bac/fun_rhodopsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043476">
        <property type="entry name" value="Yro2-like_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28286">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28286:SF1">
        <property type="entry name" value="30 KDA HEAT SHOCK PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01021">
        <property type="entry name" value="Bac_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0346">Stress response</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="270"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="358" mass="40888" checksum="B407DFC033030636" modified="2017-01-18" version="1">MSFNDLIKRSAAGNQAVSINPTSGIDIKITSHGSDWLWTCFSIFAFASVYYAFALLFKQKKNKITNLTMETNFVYLIRLWISAVMSYSYFTMASNLGYTGIQTEFHHVTTNDESFGGIRQIFYSKFISWFLCYPSLILLLFTFNFDYLLLNVKIIKFLFNYLVQWVTIEFFVIGLLIGSLIHSTYKWGYWTFACFMGLIYLFNFNCQLFHYKKNQKADYESGIASTSADGKKNKWLLCFINLFHQLIFLLYFISWSLGDGANVIQPDSDTVFFGILDLCNFCFIPLALSFLPTPELFTNIHCFENDALSIGSGAHAEKQLSIDEVRHSGDTHVDDIPVVNDNVAPAETTTEPVTPVVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REN6</accession>
    <name>A0A1E5REN6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA exonuclease NGL2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1678</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCR4/nocturin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REN6">
        <property type="interactions" value="491"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5REN6"/>
    <dbReference type="OrthoDB" id="428734at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF45">
        <property type="entry name" value="NOCTURNIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="4">
        <location>
            <begin position="83"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="467" mass="54134" checksum="047769FA18B9FD0F" modified="2017-01-18" version="1">MGKKTNKAEKKLKNAKAAEEQKQIQQHQQDLIADAHEYPKRPLLEIPRDSFCCNGNASCTNGNLPEGERNXQYTYKGLPLSIMTFNMLAQSLIQRKLFPTSGEILRWNIRRNYLTTELFYYNADILXLQEVDIAQFDKFWRPVFKEHEYECEFVYASMKQHGVAIAWKTAVFDSAPLAKQLVKFDEQLTEGVEKVTSTGNIGLILKLRFTENYKNAHAPTSKTSSVCIGTTHLFWHPFGTFERTRQTYIMLKEMRDFALKTKDSHLDGEKIPPTFFCGDFNSQPFDTPYLSITSKPVKYNDDHRMQTVLNCSLTYNYKEEKNKEYDNPKTPVPDQISLNPEHQELCLKLQNLHNNLDCAAVSLYSCGYKYVHPENSGLDNDKNEPELSNWAHTWRGLLDYILLVKPWDLNKDITKTQTLEEFENENSLKIKSFLRMPPASELPDHCLPHPNEYGSDHLSMMCEMFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RES1</accession>
    <name>A0A1E5RES1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SWI/SNF global transcription activator complex subunit SWP82</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1972</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RES1">
        <property type="interactions" value="185"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RES1"/>
    <dbReference type="InParanoid" id="A0A1E5RES1"/>
    <dbReference type="OrthoDB" id="5598844at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013933">
        <property type="entry name" value="CRC_Rsc7/Swp82"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08624">
        <property type="entry name" value="CRC_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="435"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="454"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="707" mass="80500" checksum="B8303942CF7AEBA7" modified="2017-01-18" version="1">MFSYFPAPKDEQNLFLLKKALSYVSINESTKESNVKDTHGKTQNVANTLSCFPAKAPKAPNPFAFFIDIPENEFLLANSGPGQAHDAQPLALIGDYRIALTDKLGEHKIDAVGNLQGERKYCMDSFTIPTVVTPKQPTRFLLLQRLLDILGGITKEEFQTAYPEIKIFQASDIKFKNQNKLEKPLSDYFGDWFPEISKNNYFFVHARVAFLRFNALLITGGNRILDDYWEQVFINQGFTFRDRVFLVPNKIVNKAKTILGNTGSVFSNFKHKKRNDVGDLQKAKFNLVNEKDDPFNYITEQPSMQLKKAYCNYQQQDLYFKSATDPGAEDEDDEGFQTTENGDREKEEHDADEEVSLDELPPYISRRGAGRPALATDSSQLITVTGLQHVIVPGQNITGGIELSSQYKVPRYATKIAYMQWQNSLNVPIGEVSNDRSESAMSAELNEQEDGPYQNANLEPGSSSDFPNSFASVVPTQSDSASHLAAHSGFTSGXSGKNKRLLSGLLGDDTFQQKKKAAYYVRRYFESQNGDGIVDKNVQLNIDPWKFETLPLRKSYGSKRSEMKYNSKGLPIFEDENQLKNRLRKLTPQQVMELQHQHDSVQLNKGISKLVTQRNEKWRKYWLYKAGLPLYKNDLELYENHSQRKQLVVESIKQDIENYEKVVLEKYEPDTNTDVITLEKRELGINFRNCSSIEDFRPPYAISPDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REV4</accession>
    <name>A0A1E5REV4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5REV4"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="549" mass="63390" checksum="FBE91DC72E95DEE8" modified="2017-01-18" version="1">MLFSRKEDFVDSDDVSPSQNNTALSSTSSHTSESNERDSSNLISDFEDITITDLSGNEHKHSGDEDDESSGHASEKEQNHNVDEMQLQENVLLLLERSAFLQGLEDQDRFLKKERCDDKTLDMEKEESNNKVLSDEYETSRVDSEITAGDNTAGKNDDDXDDDDDDDDDDDDDYESLGSNDVYGTSDSLQSDDPSEEDELSQEGNESENEVSQEDEEEEDEEEEDEEEDDSLNVFKDKSFAKSGRTAVEVPEKCYDRIHTIVDNMRKTMNKKNRQQFEKLQVANMKASCKDKNIKFPFALKWLDMVAKFMLKNSCHKFAVQAWIDDNNKHLNSFFQHYAGFEDLNKKQIAKLNRVFKMLLCNIVKYDFQGLSLAHEENGMKKMTFADTFEKLNECMYEERASEKVPRELNMMCELSQITMSTNYGNNIHDLFTGVGTEIDKMVQKRHLKKRDLQELLVLTCCLTKAHRYRNLHLWAPMLNEYYNNKNRLNISEGRAMAGTLDATSYREMLSKYTKSNDHRCMKMKKQKEEIPLVRKKGGKVVFSHSAIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REW8</accession>
    <name>A0A1E5REW8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C-8 sterol isomerase</fullName>
            <ecNumber evidence="8">5.-.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Delta-8--delta-7 sterol isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1739</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the reaction which results in unsaturation at C-7 in the B ring of sterols.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7 8">Steroid metabolism; ergosterol biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ERG2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.-.-.-" evidence="8"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REW8"/>
    <dbReference type="FunCoup" id="A0A1E5REW8">
        <property type="interactions" value="149"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REW8"/>
    <dbReference type="InParanoid" id="A0A1E5REW8"/>
    <dbReference type="OrthoDB" id="347124at2759"/>
    <dbReference type="UniPathway" id="UPA00768"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006716">
        <property type="entry name" value="ERG2_sigma1_rcpt-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10868">
        <property type="entry name" value="SIGMA 1-TYPE OPIOID RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10868:SF1">
        <property type="entry name" value="SIGMA NON-OPIOID INTRACELLULAR RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04622">
        <property type="entry name" value="ERG2_Sigma1R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="223" mass="25375" checksum="846D50BC9DA6A992" modified="2017-01-18" version="1">MNLLRITLLLVAVYSFLNRFLYTWLPTNYIFDPKVLNTICNSVIAKHNDLSTQESTKDFFVDLRNELANTYGEQYINKYVDEEWVFNNAGGAMGHMIILHASITEYLIIFGSAVGTEGHTGVHFADDYFTILVGEQTAALPYALKPEVYKPGMTHHLQKGYAKQYSMPSGSFALELAQGYIPAMLPFGFLDTFSSTLDIYTLYRTVYLTGRDMLKNLFINRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF17</accession>
    <name>A0A1E5RF17_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Heterogeneous nuclear rnp K-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2138</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RF17">
        <property type="interactions" value="251"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF17"/>
    <dbReference type="InParanoid" id="A0A1E5RF17"/>
    <dbReference type="OrthoDB" id="442947at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00105">
        <property type="entry name" value="KH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="20"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="146"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="263"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="342" mass="38450" checksum="44121CC2CA79DB7C" modified="2017-01-18" version="1">MSTAYTETSTQLSSSQRQTTSLQTRLLVSLKEASCLIGSQGSKIEKIKATNNVNCKLTATMPKCSDRVLIVSTPPQSTTAANNTYTENVQELQANMPTFQGXNAAIGDFLQILVDEMPLDAKSNQQKLYNYGPLNHIMPLPENCSHLGYLRLILNDSQISIIIGEGGSRIKGLLAKYEDSVRMVASKNYLPNSDDRIFEIQGEPAYVVAMLNDIDTLLMETSGTINNVFESELFDENLENTETNNAESGNYRQRTKKHYSPHFNYSRTFNIPNEYVGIMIGKNRNRINSLQNHTNTSIVVSKEQEDSPTRTFTITSTDEQQVERALKLLKSNFNKIEKTEYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF51</accession>
    <name>A0A1E5RF51_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2402</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF51"/>
    <dbReference type="InParanoid" id="A0A1E5RF51"/>
    <dbReference type="OrthoDB" id="5229455at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF43">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G01850)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="106"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="222"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="113"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="698" mass="79648" checksum="8C22B5761B3853DF" modified="2017-01-18" version="1">MDQRDIIIENGISMDKQPVKSKSAKKKEKSVKPKTKFKNLVWGGRPFKKKVVKSKIPNTTLVNGITVVDKSIFSSNTAAHNDGTGEQNSAGTKSFVFREGFQPKKFGKQLEPSSRRDWTEHQEHTXPPQKTTSIPYIIEFSEKDYGEKKSTKQSSLEQIPSPAADHIDEISFPSIFADNQQMDVTHSGNSAQDLDSTEPEAEALDSEFGVSLQVPKLNAFDSLNLNLRLEDLAAEFEELEEALEEVNATQQPLQLTKNRATNQAQFFTINPSFTTHNDLLDFFFKESCKMFVPTPFEFYRKNPFQTILPNLFSADPTLTNLAVVFGSNHRKRMHDPSHTDLIMCSQLNQEVSHISNYLQQASLNNNFSVPNSFLCSILLLSCFSTYFLQKNWRVYFQHFKKLIWKKYPLKNGSIKIAPEQATSTPDETQFLLNWFNYMDVISTLSSVDLLKGNSPSGTPIKQITYKYDLHSLETLRLNLQDIEYFSGIDIHVLYYLDKISKAILVADSKNMTEEENLQTLLNLKRNLTSYLMDSETQRDYIQQVYYLNSDPTGGHGPKFTYESATLANYRALRATNLIFGLNGIIQLNRRILKMSFSDLEVKSLLLKCLAIINDKIPPASPSSQCILSCLFTIGCDLIDDELVSYRHIIMDRLDALMQRGVSAAAEARNIIYKCWLYKDMKNWWDIMFEEGLDISFCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF55</accession>
    <name>A0A1E5RF55_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF55"/>
    <dbReference type="InParanoid" id="A0A1E5RF55"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="124" mass="13959" checksum="ABEE3507F8D30944" modified="2017-01-18" version="1">MSTKCTFPERFKCKLQIKALRRKSGVLSKKTAKHKTSTFIKKMSRVISKIKTKLGTEKDKDTKKSQTSTTSRTSSKSTLKTSKNSKLSSKTERVGGSNAESNLDNLNPREAARRAAEERYKTQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFA6</accession>
    <name>A0A1E5RFA6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">[NU+] prion formation protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1864</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFA6"/>
    <dbReference type="FunCoup" id="A0A1E5RFA6">
        <property type="interactions" value="296"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFA6"/>
    <dbReference type="InParanoid" id="A0A1E5RFA6"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18626">
        <property type="entry name" value="CD_eEF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.990:FF:000002">
        <property type="entry name" value="mRNA export factor elf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000193">
        <property type="entry name" value="Probable Elongation factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015688">
        <property type="entry name" value="eEF3_ABC2_chromodomain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047038">
        <property type="entry name" value="eEF3_chromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056891">
        <property type="entry name" value="NEW1_HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF14">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24984">
        <property type="entry name" value="HEAT_EF3_GNC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24987">
        <property type="entry name" value="HEAT_EF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24988">
        <property type="entry name" value="NEW1_HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0034">Amyloid</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0640">Prion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="589"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="831"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="8">
        <location>
            <begin position="961"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1138"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1193"/>
            <end position="1222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="38"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1207"/>
            <end position="1222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1222" mass="137704" checksum="F2A37CC1589D20D6" modified="2017-01-18" version="1">MPPKKFIPLNDYLDKQPEDPRKVKNQFGGYFNKPQGQYNTNNNTNNNYNNNYNNNNNNTSNTSNKSYSNNTTNNYNNNNNGYQPYINNNNNNNNNNNSNQAGYQAYRANNNGYQPYINTNATSTYNPNDREGSITPKEFQKTSSSASLSSTLSKLEIKDDTPITDLLNDLAMQATIQDCKKKLVECIDIMKQENPTDPGMLVSQWNVAGMISKLNKPKNSALVRESSNLFISLLASQLISGQYPFECYLLELVPLALDSFSDKSDSVKREAQHALDSIYGCFPQEGLVARFLPTICEFLEDSSAKWQSKVQALKTIDKLREECSNDMLELSFKNTIPVLIDMSTDFKPELNKQGYKTLFQFVSILDNLDLQPRLELIVKTLQDPAKVPDCIKALSSVTFVAEVTEPVLSLLVPILNRSLKTIGSSSQEQLRQTVIVVENLTRLVNNHIEIESYIPLLEPGVQKVVDTAALPEVRELATKALSVLKDDTELKNGKFPGRITAEQAKNFLLNADLGDFKSTLEKQDNFVVQDYLTNLVMMDANVNDWQNLKTYFSLFINDSEKVDHFVKTIRKTFDHKRGVSNEDDEEGAVEIVNADFSLAYGSRMLLNKTNLRLLKGHRYGLCGRNGCGKSTLMRSISKGQLEGFPTADQVKTCFVEHKIQESEADMDLVAFIASDPELDSSSNEEIADALEKVGFNEERRTQTVGSLSGGWKMKLELARAMLLQADVLLLDEPTNHLDVANVQWLKNYLLEHTEITSLIVSHDTGFLDAVCTDIIHYENKKLAYYKGNLHAFVVQKPEAKAYYTLTDSNASMRFPPPGILTGVKSNTRSVCKMTDVSFSYPNSSKISLEHVSASLSLSSRVAVLGPNGAGKSTLIKLLTGELVPNSGKVEKHPNLRFGYIAQHALQHVNEHKEKTANQYLQWRYQFGEDREVVMKESRKMSEEEKDMMVKKIDNGDGFGEREIEAIVGRQKFKKSFKYEIKWKFYKPKYNSWCSKEYLEKEGFGKLVQKFDDFEASREGLGYRELIPSVITKHFEDVGLDSEITNHTPLGSLSGGQLVKVVIAGAMWNNPHLLVLDEPTNYLDRDSLGALAVAIREWSGGVVMISHNSEFVGALCPEQWHVADGKLVQKGIAQVDQSKFEDGGNENAHGLNVSKPADDDDNFLANLKVKRRKKKMTRNEKKLQVERRRLRYIEWLSSPKGTPKPVNTDDEDEDDEEEEPKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFE1</accession>
    <name>A0A1E5RFE1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein FUN14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFE1"/>
    <dbReference type="FunCoup" id="A0A1E5RFE1">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RFE1"/>
    <dbReference type="OrthoDB" id="3990500at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="228" mass="24555" checksum="90DA98EFA0AB9029" modified="2017-01-18" version="1">MFRNGIHASFRRGFISTPKTQFTTLHSKNLFASKQSLKRIPFLLSSSLLLLAGGVTCSSGFANARAIHNESPSKPYPLSAEITPADAVNNFNSDTQRERRKKVYRQITTGSLFGLLAGTVFGKLSRVIFVICGSGMVLFEWLLTRGIVQRVTGSSGANGSGRLLGLFGLNGLTEELHDLAGTSGSNGNKSGLGASVNQFVKSTLAYMKVFPAFKLSFFLTFALAAYNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFE3</accession>
    <name>A0A1E5RFE3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mitochondrial inner membrane protease ATP23</fullName>
            <ecNumber evidence="8">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1719</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Has a dual role in the assembly of mitochondrial ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion inner membrane</location>
            <topology evidence="1 8">Peripheral membrane protein</topology>
            <orientation evidence="1 8">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the peptidase M76 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="8"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFE3">
        <property type="interactions" value="497"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFE3"/>
    <dbReference type="InParanoid" id="A0A1E5RFE3"/>
    <dbReference type="OrthoDB" id="285308at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019165">
        <property type="entry name" value="Peptidase_M76_ATP23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21711">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21711:SF0">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE ATP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09768">
        <property type="entry name" value="Peptidase_M76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4 8 9" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="268" mass="31122" checksum="FEE3C28F2809DDF2" modified="2017-01-18" version="1">MSGSDKGNSXDSGKNINGFEFWRRTLQYQTGLGLSPEEKVQYEKDYSAQLERKKCEECYSMRDWMLQYSPTVIFMTQQIEKLARSDIKFKRIIEELDSKIGMNPEHPANAQGSAKAGSTEIFKPSRQIVCDICPDMTKTGGFHPEFGILLCSNQIRDKWHLEDILSHEMIHYYDNLKFKVDWLNLKHHACSEIRASTLSGECRMMRELKMKGPQFKFASGFQECIKRRATLSVMGNPNCKDKEQAEKVVDEVWESCFNDTRPFEXVYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFK3</accession>
    <name>A0A1E5RFK3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Cyclin-dependent kinases regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2283</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the CKS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFK3"/>
    <dbReference type="FunCoup" id="A0A1E5RFK3">
        <property type="interactions" value="583"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFK3"/>
    <dbReference type="InParanoid" id="A0A1E5RFK3"/>
    <dbReference type="OrthoDB" id="440676at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.170.10:FF:000002">
        <property type="entry name" value="Cyclin-dependent kinases regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.170.10">
        <property type="entry name" value="Cyclin-dependent kinase, regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000789">
        <property type="entry name" value="Cyclin-dep_kinase_reg-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036858">
        <property type="entry name" value="Cyclin-dep_kinase_reg-sub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23415">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT/60S RIBOSOME SUBUNIT BIOGENESIS PROTEIN NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01111">
        <property type="entry name" value="CKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00296">
        <property type="entry name" value="CYCLINKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01084">
        <property type="entry name" value="CKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55637">
        <property type="entry name" value="Cell cycle regulatory proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00945">
        <property type="entry name" value="CKS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 5" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00068939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU311113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ85363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="130" mass="15517" checksum="748865975BAE7FF4" modified="2017-01-18" version="1">MSYYDTHPFQGRRLTDQERARVLDYQDSIHYSPRYSDDTHEYRHVMLPKAMLKVIPTDYFNGETGTLRILTEDEWRGLGVTQSLGWAHYECHTPEPHILLFRRPINFEQELKAQQQALLQAQQLKQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGG8</accession>
    <name>A0A1E5RGG8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1375</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGG8">
        <property type="interactions" value="104"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RGG8"/>
    <dbReference type="OrthoDB" id="4067878at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071986">
        <property type="term" value="C:Ragulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032008">
        <property type="term" value="P:positive regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001919">
        <property type="term" value="P:regulation of receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028209">
        <property type="entry name" value="LAMTOR1/MEH1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15454">
        <property type="entry name" value="LAMTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01262">
        <property type="entry name" value="LAMTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="4" id="KW-0564">Palmitate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="111"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="37"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="194" mass="21985" checksum="00F67197CBFE073D" modified="2017-01-18" version="1">MGLIFSCCLKNDENSEEQDPLLQQQKLLQLRQDQEFLQQEEQRKFMEQEEQKILKRENELTEIVNNTNDSFVDISMINNCTSTMNETVTGPXARLLEGNSNENQVNTIDTNSQTISRKDSVLSPQSTAATSTDTANNKSKKGQFTYNQLDVKSHLPNEKREALVRAWDAVIGGYSSQILQLPQTSNFGPLIEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGQ6</accession>
    <name>A0A1E5RGQ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Boron transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1595</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGQ6"/>
    <dbReference type="FunCoup" id="A0A1E5RGQ6">
        <property type="interactions" value="394"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGQ6"/>
    <dbReference type="InParanoid" id="A0A1E5RGQ6"/>
    <dbReference type="OrthoDB" id="1735926at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080139">
        <property type="term" value="F:borate efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050801">
        <property type="term" value="P:monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.570">
        <property type="entry name" value="Helical hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF82">
        <property type="entry name" value="BORON TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="267"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="313"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="457"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="496"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="522"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="6">
        <location>
            <begin position="4"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="6">
        <location>
            <begin position="192"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="6">
        <location>
            <begin position="422"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="583" mass="65358" checksum="F3FB6FC462C54455" modified="2017-01-18" version="1">MLAPFAGIRNDIKDKLPEYKSDWLDAWDYRVIPSVLETYFSTLLPALAFSQDLFNRTQTYGVNEILLGQGMAGIVFGIMGYPLTIVGITAPVSIFNYTTYDIFKVTYYEKYKDMSYLGFMFWVYLWSGIMHFLVAVFNGVTLLRFVSNFPCDIFGFFINVVYIVKGCVLLGLTFNKNETATDVADGFANVTIALCMAVFGTLVKSIVHTTFFNHKIRVIISDYSTVLSVIFWSGVIHFGNAFSKVSFAKLPITKAFYPTANSQTWLAYYKITTGQVFLALPFGIILTILFYFDHNVSSLMAQRTQYKLKKSSTFHFDFFLLGATTIVAGVLGVPAPNGLIPQAPLHTESLLVWQNKRYQPLKESDKDVEKVLTNASDYQTASMGDAEQKNISVGDPSNTAEQELLSDNGELEPEVESDLYVERCVEQRFTNTFQGIMSIITMCRPFLICLNQIPQCVLSGLFFIMGINGLLNNTITHRICFIVSDKKTGPLTNIPLKKLVLFVGSCLCFAAAEVGISNSRGAIGFPLILLLTVVFTFSFPKFLNEKEMSILDPIVASPHTLKNLLPKNLKQEKNFRIFSNSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGW2</accession>
    <name>A0A1E5RGW2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">DNA repair protein RAD2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g1046</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the XPG/RAD2 endonuclease family. XPG subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGW2">
        <property type="interactions" value="544"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGW2"/>
    <dbReference type="InParanoid" id="A0A1E5RGW2"/>
    <dbReference type="OrthoDB" id="31113at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004520">
        <property type="term" value="F:DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09904">
        <property type="entry name" value="H3TH_XPG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09868">
        <property type="entry name" value="PIN_XPG_RAD2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001044">
        <property type="entry name" value="XPG/Rad2_eukaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16171">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-G CELLS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16171:SF7">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00066">
        <property type="entry name" value="XRODRMPGMNTG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00842">
        <property type="entry name" value="XPG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="14">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="13">
        <location>
            <begin position="768"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="394"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="633"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="399"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="460"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="510"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="548"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="573"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="633"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ86149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1022" mass="116140" checksum="894B5A23EEE05934" modified="2017-01-18" version="1">MGVHKLWDILEPSSKPVRLESLNDQRLAVDASIWLYQFLTAVRDKEGNSLQSAHLTGFFRRICKLLYFGIRPVFVFDGAVSPLKKDTIKKRNERRQGKRDDAAKTARKLLALQLQNSMGSSGDKSTLRKAASPASVTRSSKKPYVDYKDEWELPTLEEFKYNKSDGRLSSMEEYQDVLNQIDDELTNVDLDSINPASKEFGKLPASTQYMILYALRMKSRLRMGYSKEQLEEIFPSSMEFSKFQIDMVQRRNFFTQKLLDSTGLHGQSYLSTGKIANEKNKEYQLLRTDTGWALSLENDDGSSVKKAIKLDDEQALDDDEDDGDDLEWEEVDVDKAPKKEVIPDYSIKASLLASGKKISAHDRXGGQAFLDRRFESPSQKTYHSPYKQTVEYIDDNDDVSDKVASTDKPKEEIRNTELEASGANNKALIADDNGDDDEDDYKTLVQEIQLMHSMKESKKQXQAKSNLSSVLRKEAVGNPQESRVDDQSDDNSNKKSASGNYLDRADEQLHQNGLQKRSVTTEEPVNDDSPTEPRKKKSKPVVPLPTWFDSNNAVDQSRTNGFELFNNEQDANAEERQEEKRKQEIARLTPKTALDFSQWDNVEPDRVILDDDDDISSNTIASVKDNTLETVEKNDSIFSEPERAENSENSAASDKADQMGIFGIEAQPAAAAAAIDVLESPKDFAKDSGNHEYSEDEEEDLKEQMEKEENEYNTFLKPALTSSQVASAFIDNNEPEDYTELLEKRRKQKRDADEVTTAMIQEVQELLSCFGIPFVTAPMEAEAQCAELLNLKLVDGIITDDSDIFLFAGDNVYRKMFQEKNYVEFYDNCTIKDKLGLERMSLIELAHLLGSDYTEGVKSVGPVSAIEILAHFGSLQKFRDWYNESQFNEKKLAEEKGFEKSLRKKLVAKQVMLDTETFPSTRVTEAYLYPVVDQDKTNFVWGNPDLGQLRALLKGYVGWTKERSDQVLIPLIRDMNKRKKEQKQKRVTDFFHKDYSSSNSIAGVGKRMESAMVSLRKXRGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGZ0</accession>
    <name>A0A1E5RGZ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1435</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGZ0">
        <property type="interactions" value="150"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGZ0"/>
    <dbReference type="InParanoid" id="A0A1E5RGZ0"/>
    <dbReference type="OrthoDB" id="410920at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="353"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="130"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="276"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="160"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="296"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="695" mass="78247" checksum="1F066D6EB52A41FB" modified="2017-01-18" version="1">MQSAHQSESSLQETSLERTRPNLSVGTQLAAQTFEKHKYDKENXLKASEEQQRYTNASDQRPTIDTXPVAQDRSDSIYDILPTPLTPYRADQSERILGTSDKIFIPKRTAHSKSDSGSLLSGFGKVSPLKENANTSNSPVMLPKRRIAGSSTTSLLSKGQPEHKFSDRRNFSFESNQGRSDNSEYEDECHDSISEIQQTSFTGISGTTTNPFLNSKRNVSVLXNTALNTSQNNAQVLCKIVDHPTIMPNVVGNKQNXVTELYQTNRVVSNLERPESFISTSPRKRNISLPLGTPAESPTDTTVXHGDLNTTVRPSKIYDTDXEDGFFTLDVVNVWELTPQKDVSGKIGKRRKYKKVRKLGSGNFSEVFLYVKENGSNMTNNEVACKHIKYPAALRSFAKDSANYKETLSKLESSLTRELYVLNKFKHQHPNIISLLGINNMDFLANPRPLTAFKSDESQECPKINDLPECYIFTDYCSGGDLLKFAKENTLSINMIQAVFKQIVHGVQFLHDNLIIHRDLKLENVLLTCKYEELAQLMSVAKTEALPKDLIKIADFGLCKEIRPDELCTTRCGSEDYVAPEILMGLEYDGRLTDCWALGVILYALLEDFLPFDLPARSNGSALTKQSAIKRNRPASYRIVRCDWKWLKLRDCGENDGANDADLLQSQAKEVVKHCLIRRTNRWNIHTIAQSDFLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH03</accession>
    <name>A0A1E5RH03_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Vacuolar aminopeptidase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1437</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the peptidase M18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH03">
        <property type="interactions" value="229"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH03"/>
    <dbReference type="InParanoid" id="A0A1E5RH03"/>
    <dbReference type="OrthoDB" id="9880441at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05658">
        <property type="entry name" value="M18_DAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.250.10:FF:000001">
        <property type="entry name" value="Aspartyl aminopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.250.10">
        <property type="entry name" value="Aminopeptidase i, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001948">
        <property type="entry name" value="Peptidase_M18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023358">
        <property type="entry name" value="Peptidase_M18_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570">
        <property type="entry name" value="ASPARTYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570:SF4">
        <property type="entry name" value="VACUOLAR AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02127">
        <property type="entry name" value="Peptidase_M18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00932">
        <property type="entry name" value="AMINO1PTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101821">
        <property type="entry name" value="Aminopeptidase/glucanase lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9 10" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
    <feature type="unsure residue" description="D or N" evidence="10">
        <location>
            <position position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="525" mass="57605" checksum="A4716229328E3223" modified="2017-01-18" version="1">MSAQSTTDAILQLKKTLEQLALSQQTLSNTNASTSTGHVTSPEPVQYSKEHLYAYSEKYLDFTYSCPTIYHVVSSMSKFLTEKGFVYISEKDLGVWEKLEIPGKYYTYRNGTNLCAFTIGKDWKPEYGVGVIGSHIDALHAKLKPASIKSRVGGFELLGVAPYGGTLNELWVDRDLGIGGRVIVEEGEGKDQKFVSKLIDSTPHPIARIPSLAPHFGEAANMPYDKEDQAVPVIGYFSADDENEPTDKPTDDEKNSPLFGKHSIHVLRYVAQLAGVKVSQLKQLDLELFDVQKGVFAGIKNDFIIGPRSDDRLCSFSALTSLIEYADATDLSKDSKLNVVCLYDNEEIGSLSRQGAKGGLMESTVSKIISSXFVKDSDATSTIKVCFSNSFIISADVIHMDNPNFTTVYLDNHKPKPNVGLTISLDSNGHMXTDVVGTSVVEEIGRINGDKLQYFQIKNNSRSGGTIGPSIASQTGARTIDMGIAQWSMHSIRSSTGSKDVGLAIKFFKGYFESWRSVYDKFGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHC3</accession>
    <name>A0A1E5RHC3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1458</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHC3">
        <property type="interactions" value="70"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHC3"/>
    <dbReference type="OrthoDB" id="19981at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021013">
        <property type="entry name" value="ATPase_Vma12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11712">
        <property type="entry name" value="Vma12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ86301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="146" mass="17354" checksum="7FA13176C56AE30E" modified="2017-01-18" version="1">MFNIKITPEIRALAEKQHVKLDDNXSLKLSILLEIIEKDSQFSKQHEDLQKVLFSEDNPLTFVYPKPYVPGSSYTKEFKEQLARLKMQEEEREYRXMVSKTEEKFSLKNGFQNISNYSPDPANKDELTPIEINKQIKEQPLVYWFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHD4</accession>
    <name>A0A1E5RHD4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1071</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHD4"/>
    <dbReference type="InParanoid" id="A0A1E5RHD4"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF427">
        <property type="entry name" value="HEAT SHOCK FACTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="7">
        <location>
            <begin position="77"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="55"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="255"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="412"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="537"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="598"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="672"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="209"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="260"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="327"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="412"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="548"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="613"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="698"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="722"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="742"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="779"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="806"/>
            <end position="841"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="847" mass="94756" checksum="FB608BAEE437D8AA" modified="2017-01-18" version="1">MQTKTFLHQLYQILNDHQYKDLISWVTEEETAFVTMNPAEYNLRASNSSTMTAITSINNTNDNNDNNDNNNDNDNNSNNNSXNNIKSITTXTHQPTFGEVGEXDECFVFKFKPYDTEFENQILKNYFKHGKISSFIRQLHMYGFHKLNKQYSSTRTVVPNDIYYLREATPLNETKNAVGVTNNDTATADSLTNDLASNLXDNTTYNGRMDSKPTSKKKELENSVWYFSHGAKLFHKRASVKELNQIQRKSTMNKKRXLLQQQQQQQQQQQQQQQQQQQNASIXSPAAGSSPVXNPQNITHLLQXDPNTRSYTSLSGPPVPTKEEHLNTSNTNQNINEFTPDKRTHSLSGTSNFFATTLASTNITPNVNEFLKKRAISAPSITNSPTNTIFPQQYVPHSNTIIEREKTSTRNIPENXITXPNNDNNSTKKNTHQNAEAVMEEQQPEPQQISLQEQTFQMNSAQESLQTNNLYMNSILTLRQALLELTEVVESMANTNNNFTGSEEHMKVLQQLEHIRTNVLITPQQGFSNNSLYTHPQQLQQPTWPPLNSSTPATQGFTPTIRTVTTGVSTNTAQQKPQPAMPVPSSNIIINATNVSNPTRHTSFNHGWPLHQGGSNRSSAFELGSNSSRNTSYSTHAGYGHTNGPIGTKGAFQMEQQQLPYAQMFNLQSATTTENSPLAKPLRNNTETISPHDHVPSAFSSYSMTNNSTDSLNFSAPTGIVQQQQQQQQQQHQTQILPPINPNNILQQYNPQPLAPLPNRLSSIPNPPQSISNALLNPGLTSLPSVGNTSPQQMSGPSPLNARFSVHNQEPYTQSQQAFQHSQGEPTQNNATPSYDFTKSSHSSKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHI7</accession>
    <name>A0A1E5RHI7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1279</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHI7"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ86360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="191" mass="22112" checksum="9EDAE06F20181CC6" modified="2017-01-18" version="1">MXVEKNKIQLEADVTIIENEMDRLLISPPCLQDPQPTSNNYYSSSNLPKECLIWVAQIVHELEFCLNRGNATNLIVGLKVKKDSLPPSIWLEKVSEQMYXNCSSKFIVKSWCTNNFNAMRNYCEQQTEFMSLNSVQVNMLAAHLNRELCNVVKFSLNGIRYFPLKNVDLCVELELADDYRKLXQCFEKLQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHM2</accession>
    <name>A0A1E5RHM2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SRR1-like protein BER1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1247</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SRR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHM2">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHM2"/>
    <dbReference type="OrthoDB" id="551431at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012942">
        <property type="entry name" value="SRR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040044">
        <property type="entry name" value="SRR1L"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28626">
        <property type="entry name" value="SRR1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28626:SF3">
        <property type="entry name" value="SRR1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07985">
        <property type="entry name" value="SRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRR1-like" evidence="2">
        <location>
            <begin position="56"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="306" mass="35118" checksum="40EE5D140F521F07" modified="2017-01-18" version="1">MNNVVNEAEVATFETVKKTHGRNRKKGVMLKSPEEKFKENLPKFRTTIQSSDMLAELKEVLATSNISHIRCLALGNFMDDTPATYQLALLLELVQWIGFEPQATTNTVSKELENGQENSEKKLFISIFDPIFTQLEKQYLKDLGEHNSYQAWSVDDHNIWSNIGSSKILYFLPHADLELTEHIIKNDHPQFLLANHIVQHTDRFTSRQLFESFPHLGKLKSVMDVKTVVPIEDGFQTQQKSNHKRKNRRNKAGSXLALATANQIDYSELSSYFNKCVILSDFKNGLLLRDQPWQNAFSDLTLHSIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHN2</accession>
    <name>A0A1E5RHN2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATPase synthesis protein 25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1419</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Mitochondrial mRNA stabilization factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion inner membrane</location>
            <topology evidence="1 7">Peripheral membrane protein</topology>
            <orientation evidence="1 7">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ATP25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHN2">
        <property type="interactions" value="88"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHN2"/>
    <dbReference type="InParanoid" id="A0A1E5RHN2"/>
    <dbReference type="OrthoDB" id="107372at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140053">
        <property type="term" value="P:mitochondrial gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048255">
        <property type="term" value="P:mRNA stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040152">
        <property type="entry name" value="Atp25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025210">
        <property type="entry name" value="ATP25_mRNA_stabil_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28087">
        <property type="entry name" value="ATPASE SYNTHESIS PROTEIN 25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28087:SF1">
        <property type="entry name" value="ATPASE SYNTHESIS PROTEIN 25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13929">
        <property type="entry name" value="mRNA_stabil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02410">
        <property type="entry name" value="RsfS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="ATP25 mRNA stabilisation" evidence="9">
        <location>
            <begin position="279"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="14"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="560" mass="65032" checksum="3B3193B6458DFFA4" modified="2017-01-18" version="1">MLAYTCRFHSTETGLKTSNHNTATETTETTEPQPEKVNVDHEASSKTPGSSSQQPWYLRQEFAAPDMADAEAVFSLAQQDSITYPDTLXQTKDAEFVKTLAXHLQSKLGLSNIIVFDMQHGDRDRPMVGKEDIIIVASAKSGRHCIGSVESLRKELKHTWEIDGGGINVEGIVSSTLRKKEHKRLQRKGGQLKRPTEWFNKASGDWIVIEFTSKQGCRVMLNVLDEQKRNYLNLEELYCKEEDLEKYTTSKECVELDRESEFSYTKQQVANEEDDILLELRKQLAQKNSSAVNKDFQKDAKRHYSTHKQGKFVEEKQEITALNQKWMALSQDHLPNEREWELRLLDLQKIMEHSENIYFYEQLFKNYLDVKLLMGERMKLSELLKINILCISHIYKVTEGNFDCEELRSRLTNKMMVKLLRYFDFVKSVQTMSIAKFLLLSLKQVSGTSKFYALTNLIQLTHQNSIPQDLKNQILRELVKNGDFIFLQKFWSVDTNRFDNLIESWTVLVKLLEQAEMSYTSHIKTLVKQELLSLLQRDLCVFQIDLNNAEIYSRLLKLTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHN6</accession>
    <name>A0A1E5RHN6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1551</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHN6"/>
    <dbReference type="InParanoid" id="A0A1E5RHN6"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ86395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="401" mass="46605" checksum="49BA44C8ECF48874" modified="2017-01-18" version="1">MGFIVAGVNTREIKRADKNNSTILSKSAYYPYLHRYRKLSTERNFGSQKHVSDDTYTDRPNVSLKKHKGSITAHTETADEVQKRLDRELNVVSRKQFLRPAIFLELPDEIIDQVMLMSGRNSNLPILNKQLYAYFNQSDIHARMMNQFVKFNYTRDNGATMVFDLRVFNCKRCRDYLMDVGLVRAGETKIDVFKRAESMSYIFNKPISSFINEEGTRKDPKSGSEVSFLVENNFPEYFLKNEDDFQKYFVPFLKETKQNAVFEKIMENPLTKVILNHPETVINMLITKTYETLSEGDQMREMFMKVRNLAISETEQQYVSDFHLSATPLVLAVQRHGKHKDKIHGLLQSLLHLYEETHNTESLSDDTLWFLLISDSKLQMPNEMIWNAGGRPSAAVMNNFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHP6</accession>
    <name>A0A1E5RHP6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulatory protein ASH1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1244</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHP6">
        <property type="interactions" value="277"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHP6"/>
    <dbReference type="InParanoid" id="A0A1E5RHP6"/>
    <dbReference type="OrthoDB" id="2162994at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="3">
        <location>
            <begin position="461"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="196"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="328"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="336"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="349"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="421"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="443"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ86405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="563" mass="63235" checksum="C153C48BFB68A5B2" modified="2017-01-18" version="1">MNQIQLPSLKNLQLLPNPNIQESSYLYPDTSEATPLWKQTLMSWCKETSYEQYLQINAFLNMGVPLPKHSYVTKTSPYSPAQDPHYTYHSHRYQQHMTNQNEQISSNCEQNVQQPQNXQQPQSFQQPAQRPNVSLNVLANAAVSVLDVNLPVSVTSSKDQFYGLSESSGSQYDLHDFNKSSKNTMINRYGYHPVNVVNTKEPSNGIDQSSDSSVSSTPCRSTTPPDSPQQNLLVFTPVISERMIDSMKITKSSKQKHKKTNSFKARQMKKILTNREVLNQDSLPVNIVKYDDNRDLLEYRKNNIQRLQQMEREALXATPKMTPGLKMTHSPTCNSHSHHHKAHPRHTPFGSQAQHQEQQFTPLNPNFLQMPQLLPSPQDVMTPPTSPQHTPASLVVEQKGPSPVEQSPKDLIVGPPPTAPSQPQQVPTFNTPKSASNLTAQSPKKHGSGGKSRRSSNEDSAKTPRTCISCGSIDSPCWRPSWSYNKHEQLCNSCGLRFKKTSTRCLNRECKKIPSKGELSIMKSNGYIQQYVTDPTTHESVLQHGLECLFCGGVTETVQKPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHU7</accession>
    <name>A0A1E5RHU7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">AdoMet-dependent rRNA methyltransferase SPB1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1652</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
            <location evidence="1 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. SPB1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHU7"/>
    <dbReference type="FunCoup" id="A0A1E5RHU7">
        <property type="interactions" value="1171"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHU7"/>
    <dbReference type="InParanoid" id="A0A1E5RHU7"/>
    <dbReference type="OrthoDB" id="1287559at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016435">
        <property type="term" value="F:rRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000004">
        <property type="entry name" value="AdoMet-dependent rRNA methyltransferase SPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03163">
        <property type="entry name" value="RNA_methyltr_E_SPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012920">
        <property type="entry name" value="rRNA_MeTfrase_SPB1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024576">
        <property type="entry name" value="rRNA_MeTfrase_Spb1_DUF3381"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028589">
        <property type="entry name" value="SPB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF13">
        <property type="entry name" value="PRE-RRNA 2'-O-RIBOSE RNA METHYLTRANSFERASE FTSJ3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11861">
        <property type="entry name" value="DUF3381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07780">
        <property type="entry name" value="Spb1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3 8" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6 8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 8 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="10">
        <location>
            <begin position="25"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3381" evidence="12">
        <location>
            <begin position="236"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA methyltransferase SPB1-like C-terminal" evidence="11">
        <location>
            <begin position="606"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="486"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="565"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="362"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="496"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="510"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="565"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="599"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="632"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF11861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ86457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="833" mass="95371" checksum="687A59B69C3D4423" modified="2017-01-18" version="1">MGKTQKKNSKGRLDKYYYLAKEKGYRARSSFKIIQINEKYGHFLEKSKVVIDLCAAPGSWCQVASNLCPVNSLIIGVDIVPMKPMPNCITFQSDITTEDCRSRLRGYMKTWKADTVLHDGAPNVGLGWVQDAFTQSHLTLQALKLAVENLVIGGTFVTKIFRSKDYNKLIWVFQQLFEKVEATKPPASRNVSAEIFVVCRNFKAPKKLDPRLLDAREVFEELPDGPANNEAKVFNPEKKVRKREGYEEGDYLLYHTANIMDFVKTEDPIDMLGGLNKFTFDNEDHEWKILKKMDLTNKEFFSCIEDLKVLGRKDFKMILRWRKQARDLLDLDKEDDEVETVPLNEEEQIEKEMADLQEKTRLKNKREKRKKNETKQKEIGRMQMNMLTPHDIGIEAASLGRESLFNLKTAEKTGILSDLAKGKKRMVFTKDELAMDNDIYLEDGVLPDRDEYIDADNLENELDVMYDQYKERKIERDARFKAKQAREEADNWEGFEESKENVQEQKDYISDDDDDVDMDQGDSDDDEAINSLISKIKGKSGDKALSTKARVLFNDPIFDGVEADFNTSLKNTSPSSSQQAISKPKTNKVSKKQEQTFSSDDDDESGDNDDFEIVANENDDDSEIDSDYDSEEDRKEDAKRDHAKDIEIATVRAMTLAHEIALGKKSKHDVADEGFNRYSFRDSDNVPEWFKEDEAEHSKINKPITKEAAMAIKEKLKALNARPIKKVAEAKARKKFRAANKLEKLKKKAGLINDDSDKSERDKADEITKLMKKVNKKAKTRPKVTLVVATGGNRGLSGRPKGVRGKYKMVDGVLKNEQRALRRIAKKNSKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI17</accession>
    <name>A0A1E5RI17_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI17"/>
    <dbReference type="InParanoid" id="A0A1E5RI17"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="2">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="2">
        <location>
            <position position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="741" mass="86856" checksum="F1A88A6FFAE48779" modified="2017-01-18" version="1">MATVDXIINELHAVANLPKGTMITPADSKNLDELITILTELVKLEKISSPGNLIKLFEIVCFANNYSISTKRYFLNNLMIPKNKHVLQSCVKDMLQCFAKVSFFNTNENNFIKKSKKRSSSQKYGEQSSEEEKSDNEDDFEDPLYSMKTETEEDPLLKSKSLQHDILHWFICTFPLWEMSIHQAENTTFYSALYSKFFYLITYDHLRANVITLLVLLTTRINCKPFRARIMIHNYKKSKDYKFLILYKLFHKLLPIGKNENEYYLNNCNRRIELFIKRKIKDMESFVKTHYITKSNQHSQLWSTYDXCMLHGNXALLLDQHXDDNSDHXENKNNDIFSNKGQKEDLAKGKIXSAEINSLDEFIKLYPKLTLSSKLIPNLHRINDYSLLNVVLNPSQTFEEELAVTALYHLDKKNFTNKTLNLALYYLYELFNFKGFQQYNSKTMYEKNLISPYQHSLXDLSPLLELPSMDMESIYFLISTITKILLQHQNYQEYLHYIRFFQAILFKILNKSXIFQYGRYLSLSFVHIFQLILRFEQEAVNDSNISTLLWDPADFYTLITKLNDPLILNYVCKVVVKYRTFIEESCLSDQTINNFNATVITLANALWKNTNSIYKKGVFGIKGPFVTQVIESLYNFDTFRIKKKDFFSLAGLCALQFTFSEKFLEILNNYNVARFPYPVTEAGYLQFLKEMQEHGSVSVLFTEYPTFEALKVQILLNYPYEGIKQILFTSVKSLRKYAQTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIB6</accession>
    <name>A0A1E5RIB6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein MPA43</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1554</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RIB6"/>
    <dbReference type="FunCoup" id="A0A1E5RIB6">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RIB6"/>
    <dbReference type="InParanoid" id="A0A1E5RIB6"/>
    <dbReference type="OrthoDB" id="203824at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019150">
        <property type="term" value="F:D-ribulokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019321">
        <property type="term" value="P:pentose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43435:SF1">
        <property type="entry name" value="PROTEIN MPA43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43435">
        <property type="entry name" value="RIBULOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="1">
        <location>
            <begin position="264"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="530" mass="59933" checksum="3C7772B1E518080F" modified="2017-01-18" version="1">MTEVGIGIDLGSSSIRVSLYCYNDDMLLQTTCRPVPYYTNDNTNHQSWQYSQSTNEILERLDECFEELDISAYNTKALGVGATCSMAVFEADDVTPHKIFGTDSAKYPLNVVFWMDGTSSLECENLNKNALPSYTKKCLGGKGFISEMGIPKLCHLLHGLNENDSLVVYDLHKYIAKKICEKFEWSYEMLKNIPNANGIGHDGELCGWTTNVYDYIMETTGKSFSIGPIAPLLPNNHKNIRVSSFIDCYAGWFATAPKLNHSMTMVAGTSTCYLYGSTKSEFHIDGIWGPFTNIFDQQDRIKVYQGGSSLTGQLLSHVLQNHPYCLKNNYSNVVDIISLLEDYIVAYDGNDEKSLFQYESKKCFLYGDLQGNRTPFADPDLKGVYIGETLDTSLKSLAIKYITTLEFLVFQTRMIIEKFPEIETLYVVGSQSKNTRLLTLLQISFPEISIYIAKDIESTEYIGPKGAYLAAKYGSKVIDVDRSNDFQKPQLIPSYLETHARKQIFNLMNKKYEIILDIIKNQQKYNAMMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIE9</accession>
    <name>A0A1E5RIE9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dihydrofolate synthetase</fullName>
            <ecNumber evidence="7">6.3.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1470</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>7,8-dihydropteroate + L-glutamate + ATP = 7,8-dihydrofolate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17839"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.12" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RIE9"/>
    <dbReference type="FunCoup" id="A0A1E5RIE9">
        <property type="interactions" value="189"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RIE9"/>
    <dbReference type="InParanoid" id="A0A1E5RIE9"/>
    <dbReference type="OrthoDB" id="5212574at2759"/>
    <dbReference type="UniPathway" id="UPA00850"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008841">
        <property type="term" value="F:dihydrofolate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136:SF0">
        <property type="entry name" value="DIHYDROFOLATE SYNTHETASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001563">
        <property type="entry name" value="Folylpolyglu_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01011">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01012">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR001563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="437" mass="48628" checksum="D780810B6F79649E" modified="2017-01-18" version="1">MSIKLGLQRIHQLIEHSPNPKLLSSLNVLHVGGTNGKGSICSYLQSILMNASKNNDSGLVEQSVHYKVGKYCSPHLIHPSDSITINSNPITMQNYEKYMSVLNDINSRHGIQASNFELLTCVAFQWFYDNKVDFAIIEVGVGGENDATNIIPGESKLVVNLNKISLDHTNLLGSTLEEIATEKSGIIKRNVGKVFINGNNTNIVKTILTNKCKSENVPYQIVNVEDVDPKLLQNAPGYQKENLAMALSNIQYLHKETTHLPRLTETDILKGMTTMEWPGRLQRLDYQYTKDKNTPVLLDGAHNEDGAKQLGDYLDNHCRKGPSTSLTFVLAMTEGKDVSGILKNVLRPKDVVVITQFDSVTDMPWIKTMSAEKLYSNVAQCVLDKNILIENNLKSTIPIALFNKINADQPLVVFGSLYLVGQMLNLHQKNVMKVTSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RND8</accession>
    <name>A0A1E5RND8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein ICE2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g846</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RND8"/>
    <dbReference type="FunCoup" id="A0A1E5RND8">
        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RND8"/>
    <dbReference type="InParanoid" id="A0A1E5RND8"/>
    <dbReference type="OrthoDB" id="5577218at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048309">
        <property type="term" value="P:endoplasmic reticulum inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000921">
        <property type="term" value="P:septin ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013635">
        <property type="entry name" value="Ice2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31726">
        <property type="entry name" value="PROTEIN ICE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31726:SF2">
        <property type="entry name" value="PROTEIN ICE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08426">
        <property type="entry name" value="ICE2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="71"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="649"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="703"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="586"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="184"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="594"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="724" mass="81967" checksum="E33D5377C9E8A61D" modified="2017-01-18" version="1">MLGTSISQAIRLLCTTVYLLNLVLTIPIAFQIGGIDCGLLFTVTLFSLYFITTTIKLLFFNHENSPQGKNYGFRFFYYLQHVCIPPILMIFLNLFSKINYPINLNTNSKNILLSKTLQNLTVAFDTLWPYYLRWVMQPWKWIILHSTPYFTLLEGVFTILAIQTIGKAFKWLNTPSPLKELPASSSSSSSSSSSSSALSNKNPSFMEQRNSEVDQDIFDKVVDSDYNNETKLLKEINEKGSISDLTSRSPSSFSSSTEKISSLNSSNSLKNLATDVNNEISFQKDGFDAEDTDAESERDDEDDDLSDIRSITSISSTSQERSNLWSILGLLFSACVITLSLYYLYKIYATPNFTLTVIEATLIGFTFSGVCGVGLYGIVSGKGSVIESSLLFAYVIRCIYEISPVLSDEAMKGIMRAIHDTWSQVNEWALIPNGLQPIVESHNFPIFEFFNNAIQSIKTSTSGTLGAIEQGLNTNDQRQHELFTHNEHATTKNLWDFIQLFTIKVLPNSIVSIYKVFGKMVKESITPSVLINLSFRLLVFYSATRIIPALQKLQQKQKLLDQQHQEIVDEYLKPLSSHLVAELEHSDTGKEENSFQNGETNSSEKVTVNQEEGETQELNTLIRKQQQKKLDQERKLQAEQGNGSGWMDLLYLYSPCILIAMYTHLILQYSRDDTLDNKICLWGCIPFLSKCFNTQAVVKIDSWGFWNWINIFTTVFLYGFELGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNG7</accession>
    <name>A0A1E5RNG7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DBF2 kinase activator protein MOB1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g832</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNG7"/>
    <dbReference type="FunCoup" id="A0A1E5RNG7">
        <property type="interactions" value="553"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNG7"/>
    <dbReference type="InParanoid" id="A0A1E5RNG7"/>
    <dbReference type="OrthoDB" id="8170117at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.30">
        <property type="entry name" value="MOB kinase activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005301">
        <property type="entry name" value="MOB_kinase_act_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036703">
        <property type="entry name" value="MOB_kinase_act_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22599">
        <property type="entry name" value="MPS ONE BINDER KINASE ACTIVATOR-LIKE MOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03637">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01388">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101152">
        <property type="entry name" value="Mob1/phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="33"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="59"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR605301-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="326" mass="37848" checksum="29924463DFFEF4A8" modified="2017-01-18" version="1">MSFLQNFHLSPGQTIKPSRGFKFNSKQQNESPNGKRGEHDLEKAGYQDNMGYGTPTKANHNIDNNNNNNNNSNINPYGNFQPPITPRNFQQPSQYHNQNNFQQQHNNPAQIVDFNYTPTHQLPFISKNSTTQVSSHKDLKKIVATTLNSEAVLEQAVKLPKGEDLNEWFAVHNVDFYNHINMLYGTITEFCSPQSCPKMIATNEYEYLWNLYPGQPPVSVSAPKYVSCLMSWCQHYFDDETVFPTKENVPFPPNFVNKYVRPMLKRLFRVYAHIYCHHFNEIFEMNVHPLLNTSFRHFCLFTKHFQLLSDDDYGPLLELVNDLTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNL7</accession>
    <name>A0A1E5RNL7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Mitochondrial N(5)-glutamine methyltransferase MTQ1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g810</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNL7"/>
    <dbReference type="FunCoup" id="A0A1E5RNL7">
        <property type="interactions" value="85"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNL7"/>
    <dbReference type="InParanoid" id="A0A1E5RNL7"/>
    <dbReference type="OrthoDB" id="269872at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004556">
        <property type="entry name" value="HemK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050320">
        <property type="entry name" value="N5-glutamine_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00536">
        <property type="entry name" value="hemK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18895">
        <property type="entry name" value="HEMK METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18895:SF74">
        <property type="entry name" value="MTRF1L RELEASE FACTOR GLUTAMINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="4">
        <location>
            <begin position="123"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ88495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="323" mass="37608" checksum="48BEA65C099CD8EC" modified="2017-01-18" version="1">MRISPKTVWKAFQKDPLLPLLLPECRTIENSITELKWIKENFNNNYTKKDRLNLIKKSCLKRQKHYPLQYILGDQPFGKLDILCRPGVLIPRWETEEWCHELIELMNENVMQKSNETMGGIETKLRYIDLCTGTGCILLLLAASLNLKDPMGIDISSKAISLANQNLQHNVSYYKNSLSQPKFLQKDVFEMDPTKENLPHFDLVTSNPPYIHDHLHSKSVKIYEPKLALYGNLEFYKNLVGKWCNQTDSFVYELGSIHQAQYVRDCLSNEEWDVGVYKDSANHIRCVYGYRKKNISVANIHWKKVFEKFGTLFAAKKKTKKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNL8</accession>
    <name>A0A1E5RNL8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative lipase ROG1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g906</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the putative lipase ROG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNL8"/>
    <dbReference type="FunCoup" id="A0A1E5RNL8">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNL8"/>
    <dbReference type="InParanoid" id="A0A1E5RNL8"/>
    <dbReference type="OrthoDB" id="5368485at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016445">
        <property type="entry name" value="Rog1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF62">
        <property type="entry name" value="LIPASE ROG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005412">
        <property type="entry name" value="UCP005412_abhydr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF676" evidence="3">
        <location>
            <begin position="178"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ88471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="659" mass="74140" checksum="DC8B0E0BDD9CE5CC" modified="2017-01-18" version="1">MLIQGFKTQSSLTIGNIDRYVVKYKLHDGNINHISEYLNASSVLKIKIHNLVHISLRAAGLVTGPYTLYCEVRPQNYHHHQRVFATATQPKFESNVQPQQTFHADLQLNDFSKEELTWTIDVISQVLLTCTATITYELSIEFENGAGMSSDRLYVTKMNTEDIWELPFSQLQNNALGKKDHLVILTHGLLSNLTTDMLYLKEEIEKSDPCCIVTGYSGNVCKTEKGIKYLGIKLANFIVSKTAQVQSHIKKISFIGHSLGGLVQTFAIAYIGLKFPLFFQKLQLENFITLASPLTGVLTDNPKYVQLLLAAGMLGKSGVDLGLASMDGYANECLLSLLPCAHTRNLLRRFKRRTVYANAINDGVVPLYSSCLLYLDYEDVMNSLEEYNNPEVSVQELKGEQQEFLQKNLVKPFSKAVSLWAPQATTTSEPRSIPKLSLLDTASHVLLQPVPPLAFIVNPSARPNIILHDRLYTAADIETIKTQESFQALLTKSKKSKKNLNYMLEVISGGSDDARHKEEVIASRWHKDLTWRKVVLGLEPDAHNNINVRRRFSNGYGWQVIDHLTTEHFIHPYTETATSNNFESTDDVSDFNWLLEPEKQGFFDAGATGLISSMSNLLDQWTGTTEEYDSTPTSHDGKKQKQNVYDQTLTHEDLDLLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV30</accession>
    <name>A0A1E5RV30_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein scalloped</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g472</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TEC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RV30"/>
    <dbReference type="STRING" id="56408.A0A1E5RV30"/>
    <dbReference type="InParanoid" id="A0A1E5RV30"/>
    <dbReference type="OrthoDB" id="10006572at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.40">
        <property type="entry name" value="TEA/ATTS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000818">
        <property type="entry name" value="TEA/ATTS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038096">
        <property type="entry name" value="TEA/ATTS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050937">
        <property type="entry name" value="TEC1_TEAD_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834:SF0">
        <property type="entry name" value="PROTEIN SCALLOPED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834">
        <property type="entry name" value="TRANSCRIPTIONAL ENHANCER FACTOR TEF RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01285">
        <property type="entry name" value="TEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00065">
        <property type="entry name" value="TEADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00426">
        <property type="entry name" value="TEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51088">
        <property type="entry name" value="TEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TEA" evidence="8">
        <location>
            <begin position="452"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="TEA" evidence="6">
        <location>
            <begin position="452"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="300"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="542"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="300"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="318"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ90807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="673" mass="75980" checksum="D14B526CC0968ACF" modified="2017-01-18" version="1">MTRLENTKNINTGHDTDNEINNTDNMTPYFKNILEGMAFEPYNHKVATTTNTNTNTNTNTNTTTTTPSQFNSFIEIFDRNDKNIYADLDAHVHNGNDQAQNHHHLEGIDVMDYGKTTRTNTLIDDPARMEDYEHDELSVMQGIDGVSQKTVWANFQTGRTMLSYSQPLKSQDHQMYPPQNYHPVKGKLNQSFSNTYNNINQQWSNYMNSPQTNSTLLGQQPMQQVHPGMAHLQHRFSHPSISSGSTAHTYYYPQSSEVDLQKGPENKSSLNNHFGYTDEWDTHTNNSDYHLAQCFQANSTRSSTSNPKDYNQAGETQSVSLVSSHSGSDNSRELLVSTTDLCIFDTKSCAKTQLQPQPQPQPQFEEPVSAKPSQFQYVTFSSPNHHSTKKNSSKYRKGDLSAISQHVKMITMPSVHIGQDVLMTPVLNTPPNSAHLAQTASSMLHTTMGDASVSASGVWSKDVCDAFFQSLAIIPKDDSSRIKLNKKMYGRNELISMYIEYKTYKKRQVKQVSSHLQVTKRRILKKLELMCGNKIKNNSITTLNTSTSSSPSPNTMGSTNTIFRSPTSGRSSSLSSMGSPLDGISKDYMNVIKNINSSGNNAYNKRENHARAVHLKTLKLIDKGIEKTEKNMIFFRKIFEPIARELELRHEDHDSRDNTLIHHPLEVEFEHTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVB5</accession>
    <name>A0A1E5RVB5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flocculation protein FLO1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g517</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RVB5"/>
    <dbReference type="OrthoDB" id="4070743at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000128">
        <property type="term" value="P:flocculation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001389">
        <property type="entry name" value="Flocculin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00624">
        <property type="entry name" value="Flocculin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184892" description="Flocculation protein FLO1" evidence="2">
        <location>
            <begin position="22"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="465"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="325"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ90834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="676" mass="71577" checksum="93D4E308A7807811" modified="2017-01-18" version="1">MIFTKLYNLSVLVFWLKNVAAAGRHGETSSTSSDPTFSTSTTSTIFSTTNAGTCPSCSTLSTLTSKEVWYFTSFTSYTGSTKSTYSTDSNVISGTTSTTYYVEXPVARASSTTTTCYDGSITSTYGTSVSTMTGTDGIETVSTLYYVETPDVHVTSYTTTSWTGSFTSTYAIDYTTFEGNDGVETIKTLYYVETPDVHVTSYTTTSWTGSFTSXYATXYTTFEGSDXXETIKTLYYVETPXARASXTTTTFISYSQSXVFLSTSSQEVSSSISSSQNITSTSSSSHISTTSSSQISISTSSYSQSNVLPISSSQSRGADTSSVQSNTPTTSDTFTTSYVQSTPAATSYNQSVIPTGSFSQNSTFTASTIQSTXXQIQNFTSSFTPSFHNVSLITIIQSTTNLTQTKPNTXTVYSASSKLPTSSAQXLITSKTTIVTSTPISSASTVSXFPSSSTILLFNTSSIPQNSPASQYSNLSXSTGFTPTSASEARSXTLVPTTSASEARSVTLVPTTSVIQXSSTNITTGASTYFTVSEYSTTSSVTTLSPTSVXTVIGTSNLVDSTSVFSTVQETSNDFEISAXEIIKTSQTXAFNTTFESLSSSTATQSSPTSFSPIXSSGVPPFSSSTSTLFSSKKTTSTSAHSSMAITVTQEAAANFIFTNHINKWLLSIVAAFLFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVG1</accession>
    <name>A0A1E5RVG1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="2">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g507</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVG1">
        <property type="interactions" value="888"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVG1"/>
    <dbReference type="InParanoid" id="A0A1E5RVG1"/>
    <dbReference type="OrthoDB" id="10264149at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05167">
        <property type="entry name" value="PI4Kc_III_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000012">
        <property type="entry name" value="Phosphatidylinositol 4-kinase alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF15">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIK helical" evidence="7">
        <location>
            <begin position="1556"/>
            <end position="1750"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="6">
        <location>
            <begin position="1866"/>
            <end position="2135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="748"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1848"/>
            <end position="1884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1848"/>
            <end position="1883"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="8">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ90816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="2151" mass="246764" checksum="9789ED380F72B116" modified="2017-01-18" version="1">MSSFRRHNFSLRGECLKKLTQLSMKQDSASGDAVDKTAPYWKATTDQLSVSYTTDRSKVYTIPLTLNELEILFAVCSSIPETEDQAANLIDNVISHYFSTCTKQQFNDICKNKFKDVHNVRPDGRSVYECLTYNLCTFLIRCYAKYSNLQSRIETMFLKNFQNYKFTTYSLCSLLGLLNAFIKTATDDAPSKLHDVVLRQFWDWNFSDGHFDARFHEQITNVLQNENYDDTLMSYFDLNYELSSFMFGERIAALQILILKNKFNFSDDYSFIEHILRLKKCELSNEKGTVKFESFLKTIKKHGSFLSDCLKFSLETLDYLQHKSMNLSTFSKVEFAFMNKDCCLQVSSLLLFMTENVKTDLNIARVVDIILEFMRAYTDDSYLFYEGFLVSIMTFASLLNYYTEQLTTELLNMFPIFISNKFVSNELVVRISKNFSLGLPPLTEDAVVSSVYDITNLLGQNSMAHLASLNQRKAVIDDSINSKFXQXFQEQPTLNGLSLDESDGNEATRKKNTRHGKFEIKKRANTLTTLQSMNIFKNFPEKXLLDNLSXQSVDGSASVXNGVATPKDDVSEETADLFRKMTLDSNDEEKNDKWKFWNNVVSALVHVASLYNDQSINVLTITILTQKFGTVSETLDSIILHKVPVLVNQVGETEFQLLLKFIQYHEKNAKSKAKITSCMESRCEISKVLHKLGPKHPLYMIYLKDLLENIVAEGENQASEMLSQVDYEGRSISQKDGLVRPELQKLASSHRSARSHGHQNHTEKTEHEVMSSGGRIMVYLKPVSLLLPGLNERKINYQKDLDESLVXLFRNSWFNMVVHGFYYPFPQLDRRLXSKSSNTHVSLSTMSGMRDSYLQVFAYNSPPLASNFPKSTRETSFEMNTILRRDTSDSNLKLQNSVIQHFHKLENSLISNNSVSNQVKTLFLSSSIMLELYRCDSLSNESVSNCILYLTDPQVKSHFNKFFQIFSLELVKWISKKTKLIKGASCRAPIDLQKLLICTSSRDVDVQRQSFECCNSFIQHCPDILIRDENSIFTMLGLLSVVFKSCVDMKQTTMSPPFKYTIHTGGAHTETIFLPMNDTWRIQTLQLMEQFYSKWISKSFKYYQDDIEILMQKFISLKTKRGNSGGRFSGHADFYADEIDYAVSFAMTHATGISSREKPILKAVFPPRTYHPSTSASVTKFDNAYKFLSKQSWSSSFWSDNMGFFSRDEIVKTRNSMVLQGLHLSRDLIEYLNLTSTLLCIGDPDQNFQDGGLQLIRELYEVSMVELSNSSINFEEQMDSLSVIKHSVDIWANVMKEKDFFKNLFLSEFIHCWSLAFPGTFENSLGXXFPVFSKTFDIVGSEYNEMEYKPSDTERIQHRDRITMVHFEKMHHVLQFWCQFXKKEYVGNGTSFTGLNGTVHSFLSLIATPRVFSTLSTHPYSLKVRLDIMEILLICIEKLQGDFMQLTQLCNWLIYDGCFWFSLPNGQSSIYGENEIEMIICLEQLQKVQTRLAYCFQKIVKFVDPLEDDAVSSKNQVIILQKMIALEIKNMRDWMSLDNSNVVGVLGDFSDGGANSIDISESLVKTCFAMDCGLCLQLLERFRLSNKFGSLLIKLLVGSSHITNSMLQNGDLILPYLLSNGSGDILKFLYKYMVFFKPIAPIQSINIFKMLISGGRNGSKNLSNVILQFNLKSLEYHDSRVVFFYIPQIVQCLRYDLSGYVEXFIIDTGKINMYFQHQIIWNMLANMYKDDEGVVPDEVLKPLLDKLYIKILNNFTRREREFYDLEFGFFNEVTAISGKLKPFIKKSKMEKKLKIDEEMALIKIKPRVYLPSHPDGTVVDIDRLSGKPLQSHAKAPFMATFLIRGSSSSETNTETTTITSTTTTTSTTTSTSTXTSTSTSTSNLHKDPDLRWQSAIFKVGDDCRQDVLAIQLINVCKNIWEHYCLDIYVFPYRVIATNAGCGLIDVLPHSISRDMLGREAVNGLYEWFLNKFGDENSMEFEVARLNFIKSLAGYSVISYLLQFKDRHNGNIMYDDLGHILHIDFGFIFDIVPGGVKFEAVPFKLTKEMVKILGGSKNSXYFMLFEKLCCQAFLQLRXQHEFIIKCIEPMLDSGLPCFRANKTIKNLQNRFVLEKDDLQAAQYMKHLINKSYESMYTKGYDEFQKLTNGIPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVJ6</accession>
    <name>A0A1E5RVJ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="7">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g458</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVJ6">
        <property type="interactions" value="158"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVJ6"/>
    <dbReference type="InParanoid" id="A0A1E5RVJ6"/>
    <dbReference type="OrthoDB" id="3971384at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF95">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Rhodanese" evidence="9">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="10">
        <location>
            <begin position="553"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="346"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="358"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="377"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="404"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="453"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ90855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="927" mass="106171" checksum="DEE2A090068A2711" modified="2017-01-18" version="1">MEPVVCKSLTALKLDVESKIQEEHYEALSFQQILDKCMISLSTYQKWWKVIEEFQVTSFDLLSEPQQKQLVLAYENAYFXSKLVVKGSAQIIPRMQEFKTLKQQQLQGKQSDLLQVYDDFMHYLFKCQPNFDTVKNFLNLHSFTKQQKLQLNQQLELLDSTDNNNTISCKLLHFILHHHSNSTLLIDIRPKLEFERCHIKFEKCISIEPISFKETYTDQDIVDRSMCTSSSKDKLLFQARDLQKFIILYTSKRSPQYYSQMVALKNILLKKSFGKTLNLQRTKVLVLGESIEQWVDMYGYESCTLGSLQDGMQHANGVDKTPLLSSPPSSPPSLTYAFQKQQQILQPKGISQGREKSNGNYSNESLKTSYPDPLLSFGNSSQIKPVRFTHNSPRSSPVPMYASLPYPQQQPQQPQQPQQPQQPYQLPTLEPLPPQQSSLSIKRSPSFRDVYNNFLSKGTKSGTTNKETSLQNRPPSQSVVDLVASYPETPAISSSMNRAQSFSLDSSLPALSTQPVVMQSALSKMMRNGTGKSSHNNSLPSASPPVPFKSFCTGLVNLGNSCYMNCIIQCLLGTPELVRLFLGDDYKRYINMRSKLGSQGVITKNFSDLIHSMFERNNGVVQPRKFKSACGLVSSIFRNNDQQDCQEFVQFLLDALHEDMNMSEPSSGRKEVSEADELRREKMTMRDASIIEWERYLTTDFSVILDMFQGQYSSRLQCQVCQRTSTTYQPFSVLSVPVPPPNAMSKNGSGSVPYKINLNNCFQEFTKVENLGRDELWNCPVCKKKQPSTKKITITRLPKNLIIHLKRFDNSLNKNSEFIYYPPVLDMTPFWFDDKPEFYKSAAKEWLPNIQQRIQEPPFKYSLNAVACHTGTLYGGHYTSYVKKENFQNQLNDTQWVYYDDASARKVKNNAEMITSSAYVLFYRKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVR4</accession>
    <name>A0A1E5RVR4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Translation initiation factor RLI1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ATP-binding cassette sub-family E member RLI1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g422</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the multifactor complex (MFC) involved in translation initiation. Required for the binding of MFC to the 40S ribosome. Required for the processing and nuclear export of the 60S and 40S ribosomal subunits.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVR4"/>
    <dbReference type="FunCoup" id="A0A1E5RVR4">
        <property type="interactions" value="1283"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVR4"/>
    <dbReference type="InParanoid" id="A0A1E5RVR4"/>
    <dbReference type="OrthoDB" id="6593433at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03236">
        <property type="entry name" value="ABC_RNaseL_inhibitor_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000144">
        <property type="entry name" value="ATP-binding cassette sub-family E member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000152">
        <property type="entry name" value="ATP-binding cassette, sub-family E, member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013283">
        <property type="entry name" value="RLI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034348">
        <property type="entry name" value="RLI_dom_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009945">
        <property type="entry name" value="PRK13409.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19248">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="Fer4_RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01868">
        <property type="entry name" value="ABCEFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="46"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="77"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="344"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00056499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00067238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00082546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ91031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="607" mass="67736" checksum="19CF535413B9CE18" modified="2017-01-18" version="1">MSDKNTRIAIVNSDRCKPKKCRQECRRACPVVKTGKLCIEVTPTSKIAFISENLCIGCGICVKKCPMDAITIINLPTNLENEVTHRYSANSFKLHRLPTPRPGQVLGLVGTNGIGKSTALKILAGKQKPNLGKYDQPPEWTDIIRYFRGSELQNYFTKMLEDDIKAIIKPQYVDNLPRAIKGPVTKVGELLKIRIEKGEEYILGMIKSLQLTHVLKRDIVNLSGGELQRFAIAMTCVQKADVYMFDEPSSYLDVKQRLKAATVIRSLLDATTYVISVEHDLSVLDYLSDFVCILYGVPSVYGVVTLPASVREGINIFLDGHIPAENLRFRDESLQFRISDASDFIESDASKSFQYPNMTKTQGDFKLTVKSGDFSDSEILVLMGENGTGKTTLIKLLAGAISPDDGEKVAKMNVSMKPQKIAPKFPGTVRQLFFKKIRGSFLSPQFQTDVAKPLKIESIIDNEVQTLSGGELQRVAIVLALGIPADIYLIDEPSAYLDSEQRIICSKVIRRFVMHNKKTAFVVEHDFIMATYLADRVIVFDGIPSKDAVANTPESLLTGCNRFLKNLNVTFRRDPTSFRPRINKLDSQMDQEQKASGNYFFLDSGSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RW00</accession>
    <name>A0A1E5RW00_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Covalently-linked cell wall protein 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g354</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RW00"/>
    <dbReference type="FunCoup" id="A0A1E5RW00">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RW00"/>
    <dbReference type="InParanoid" id="A0A1E5RW00"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025928">
        <property type="entry name" value="Flocculin_t3_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13928">
        <property type="entry name" value="Flocculin_t3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184906" evidence="3">
        <location>
            <begin position="19"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ91009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="125" mass="12493" checksum="57B4BB33B6A93D3D" modified="2017-01-18" version="1">MQFSTVATVAAVAAVASASYNETATVFDNSTTIVTITSCEENKCSTTTSAALISTATTTVEGVVTEYTTYCPLTSETTAAPVTVSSVTEAQNTTSHTVTSQSENGAAKALPAVGALMAGAAALLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZN5</accession>
    <name>A0A1E5RZN5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Chloride channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g180</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the chloride channel (TC 2.A.49) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZN5">
        <property type="interactions" value="342"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RZN5"/>
    <dbReference type="OrthoDB" id="44789at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03684">
        <property type="entry name" value="ClC_3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711:SF9">
        <property type="entry name" value="ANION_PROTON EXCHANGE TRANSPORTER GEF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7 9" id="KW-0868">Chloride</keyword>
    <keyword evidence="5 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="154"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="229"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="275"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="449"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="478"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="510"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="543"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="10">
        <location>
            <begin position="689"/>
            <end position="745"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ92344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="775" mass="86558" checksum="A359749C020D1A89" modified="2017-01-18" version="1">MPAESYSNLDTSTQSEVANFNKFTTIDWPEERVKEAKEKKRPLLSTTDLENPDLRTHHEYVNRSVWTSMHRYFNHMFRQNWTTFLTLTAMGITIGCIAGFIQIFTESLVSWKSGYCSKNWLLNKNFCCSEAKEFESQCVAEGFWINYSNGGLKFIVFVILSIVYAIISAVMVKFVAPMAAGSGISEIKVTVAGFIYNTDFYSLSTLLIKSLALPLTIASGLSVGKEGPSVHYATCCGFIIAKFFLQKGRQHLNKWSSTSSSLSQYGVNFPELSEYMVAGAAGGVAVAFGSPIGGVLFALEDIASSSDYNLSTLWKSYYVTMMGIATLKAINPFRNGKIVQFEITYDKDWHIAEIPIFLLLGVFGGVYGIIISKLNVYMVHFRKKYLSSYALQEVLILTVLTSLISYFNQFLQLDMAESMEILFHECIGEDDATWDHQLCSVSSASPFKFVQMCSSLLFATIVRAALIIVSYNCKVPCGIFVPSMAVGATFGRFISILVEKFITGPNVITAGTYAFLGAAASLSGITHLTLSVVVIMFELTGAFFYIIPTMIVVAVTRMIFANYGTGMGIAEQMIVFNGFPHLEEKYDHHLSVDYSAQDIMCSKLQTLPEHISIRDLKMVLSSSVQDKVHGFPVITIANKCIGYVKRDNLETFLYMNNIDDLTIDFNRATLGENEQQVDQLENVENDFLMDKSVIVVKSTMKITLIHDIFKKVGPKVVMVENENGDLTGLITRKDLMSFESSKHKELNGALYTFNESWDDFLWSIITKXKAKLGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S011</accession>
    <name>A0A1E5S011_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g32</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5S011"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1415"/>
            <end position="1491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1523"/>
            <end position="1577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1476"/>
            <end position="1489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1523"/>
            <end position="1557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1565"/>
            <end position="1577"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="2">
        <location>
            <position position="764"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="2">
        <location>
            <position position="1063"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1715" mass="195155" checksum="E8152E52C5DA0A33" modified="2017-01-18" version="1">MKKTVTFSSKPTDIETIAQRSPTEVNQPEPLLATQNQPQSVESGNQAADDDKAKTEINSINNVPTTTTQPGEKNFHSVNLKPILKISNIQDNCNQATNEPPHEISMFSNLVEKLAQLKASKPKEGKLLATLNEIFTCGLVILEKYHLDPTNLDTFLSTKEIFQVFSNFNYSLQFVNQSFQIASVLEMITREPDRVLQLTFMHLESLGTLMRDASFFDSKFLARIYVQIVRFYVVFLNKTNLVAKNYSNHAFRSVLQKVMKELLLNLKVLIPSDAPAVTKRSILLSTIAFYKDLNASPDIWKEFDFGESLNLLLNSLQVKDETVQSEKLDLIVAFVMRYPTQMLENTKLWFLQHVIINILADTINPFDEKVFLLIGHILMACVHKYCLYPENKHLFQALIFHIKPXELHALNNNNCLTLTFSEFEGNGETATIWGLLTSKLVYLQPIIKEKLVMDIWSALSLLCFNTPQAVSTKVDKKAALDRLSTGRVLGSHWIAVNTNAFLNFDFSXQKQALVSRRDLAFILFETLSEEKNPAKMETYITHLFELSLPENIAKSKNAKITHYYLQYFFKIGHLLSFHTYYGHLLCVSQLLKGLFDGFENLINAFPSERNKILMHETTIFQRMLQRPVPPDITSSAKKALSNFVIKLSDIDPLNXLDNNATERILXIXIRLIKTYPEVDYTSSVALLLKRXQPTYANTFALCDVFVALLSQCKNYEHLKSTLGFLFETLLSYFQQNIVSEELNETLFTRLLKQTKLLEKDFFMLLQEKLLXXNLXVDTKLRVYASLIXXSKASDNLIKXNYPKCLTNPTTDHGVLLKVLPSMIPLVNVLPTNDXIQFVVLNAQTINYDVFENINIDNWSLQEVFAFLSEYQSVNQTELPLTSDKVFLSFFEKDQSFILPVLNSIESLFTNXGTQFERLAIKSQIVFRXLKNNNFHFSSKLIELQLKDLSNLELDTAIDLLXYSLSPLVFTQYKAQFSEWVYYARIDNEHQWLKLHNLLGVVLESVHGTKDAALTNEYMRLFVHTGHWSVLFNFVTQHGVDLQALSLELLSGLACFSKEAGLQLTDLLSQASFPVVSAIMAEWISFRNVYGMLMFLDQIIYCYVQGENNWNRDEKFHMITHFNTVCYLLSTYGRWSEYQLFLDTLTNKMTFESFYTAELLCIAFMQPNFTYRKQSFQKHAAALNYSAKFKSDFICPSIKLELDREVGVKRFETYYEDAKIKSEHFISVAQTLGAENHASVSHENVDALKKVTPKQGTIAMEVIAEKENGVNDQKKNEIAHSKENELLRGPDVELSFEPDFIDSSTQILLNTQRASKEQFLPAVGEKTITHAKSSLVACKPDNGPESFETISEYSNSLNDINPQTGFRSQALQHMETTKQNGVFEENPQAKSFQLQPIPEQEQIITSSNNSTKIAVSETLNNKDVPKQLKSNASVKKSKSSGKQVVXLDSMGKEEYNVENELESDTEKEVAKSIQEXPKPEGKSKAQDNSKRQAKITYQLHSQSKVASLNENAVGRQGNETVEAKLANELEASEKEFDHHSGNSKEGSVTKKEHPRQDDLNSECDAESTSNQKGSSLLENSVLLPGKLNLARQNMSSDTQTADDHNGLKTTQKEHNFFNNDATNVILDLAPDSSTEKNFEKQTAKYESTKQNEKKHKITTDETARATKKSKVSVLQTSKVRKLVAAMRRFTGQDVENLSNSEKNIFKKEVARFFERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0J8</accession>
    <name>A0A1E5R0J8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">rRNA 2'-O-methyltransferase fibrillarin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Histone-glutamine methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g4457</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-glutaminyl-[histone H2A] + S-adenosyl-L-methionine = N(5)-methyl-L-glutaminyl-[histone H2A] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50904"/>
            <dbReference type="Rhea" id="RHEA-COMP:12837"/>
            <dbReference type="Rhea" id="RHEA-COMP:12839"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61891"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. Fibrillarin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0J8">
        <property type="interactions" value="1121"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0J8"/>
    <dbReference type="InParanoid" id="A0A1E5R0J8"/>
    <dbReference type="OrthoDB" id="1859733at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990259">
        <property type="term" value="F:histone H2AQ104 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008649">
        <property type="term" value="F:rRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000452">
        <property type="term" value="P:snoRNA guided rRNA 2'-O-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000056">
        <property type="entry name" value="Fibrillarin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000001">
        <property type="entry name" value="Fibrillarin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00351">
        <property type="entry name" value="RNA_methyltransf_FlpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000692">
        <property type="entry name" value="Fibrillarin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020813">
        <property type="entry name" value="Fibrillarin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003276">
        <property type="entry name" value="PRK04266.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335:SF17">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335">
        <property type="entry name" value="RRNA 2-O-METHYLTRANSFERASE FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01269">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006540">
        <property type="entry name" value="Nop17p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00052">
        <property type="entry name" value="FIBRILLARIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01206">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00566">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7 15" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="7"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ80428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="321" mass="33627" checksum="92AA8FC7D59EDF58" modified="2017-01-18" version="1">MSFRPGSRGGARGGSRGGFGGGRGGARGGSRGGFGGGRGGXRGGSRGGFGGGRGGARGGARGGSRGGARGGASGGARGGAKVVIEPHKFGGVFIARGKEDLLVTKNMAPGESVYGEKRVSVEEPSKEEGVPPTKIEYRVWNPFRSKLAAGIMGGLDELFIGPGKKVLYLGAASGTSVSHVSDVVGPEGLVYAVEFSHRPGRELISMAKKRPNVIPIIEDARHPQKYRMLIGMVDCVFADVAQPDQARIIALNSHMFLKDQGGVVISIKANCIDSTVDAETVFAREVQKLREEKIKPLEQLTLEPYERDHCIVVGKYMRGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1B9</accession>
    <name>A0A1E5R1B9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Xanthine phosphoribosyltransferase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3847</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1B9"/>
    <dbReference type="FunCoup" id="A0A1E5R1B9">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1B9"/>
    <dbReference type="InParanoid" id="A0A1E5R1B9"/>
    <dbReference type="OrthoDB" id="9973266at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004422">
        <property type="term" value="F:hypoxanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032263">
        <property type="term" value="P:GMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046100">
        <property type="term" value="P:hypoxanthine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032265">
        <property type="term" value="P:XMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000033">
        <property type="entry name" value="Xanthine phosphoribosyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363">
        <property type="entry name" value="HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363:SF4">
        <property type="entry name" value="XANTHINE PHOSPHORIBOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="4">
        <location>
            <begin position="11"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="118"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ80672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="207" mass="23472" checksum="F9B67D3467CB54DB" modified="2017-01-18" version="1">MTSDDKMYISYNNIHKLCKTTASKILSAGPVDVIIAISGGGLIPSRIIRSFLKVNGKNIPIQAIGLSLYENMGSEEYEETIGKEVVRTQWLDLATLEKKIDSILGKRILIVDEVDDTRTTLHYALSELEKEIEKQQEKTGRTNEKTEFSIFVLHNKTKPKKADLPADMFAEGRYYAAEEVPDKWLCYPWDAQDIDEHTKFATEQGNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1D6</accession>
    <name>A0A1E5R1D6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative aldehyde dehydrogenase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3960</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1D6">
        <property type="interactions" value="197"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1D6"/>
    <dbReference type="InParanoid" id="A0A1E5R1D6"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07098">
        <property type="entry name" value="ALDH_F15-22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000024">
        <property type="entry name" value="Betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="3">
        <location>
            <begin position="84"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="630" mass="70254" checksum="05BE2C3542628534" modified="2017-01-18" version="1">MEHKPHSVHPHNFKTNNTHFFKDQPMYVSVIATIFVVLVTSKXLLNQKKSKIPKPIKFQYPIPDEAKPHWKGKRIQKASCFQDNDASNIYSYCPATGAYLGKFPSMTTEDLDKTILKSKKAQLKWKNTTFEQRKQVLVTLYDYILENQESIAKVCSLDSGKTLVDGSMGEILVSLEKINWILKHGEDILTKVDKRPGPTHFFLKFYKGAKVVYEPLGLVGAIVSWNYPFHNLLGPIVASIFTGNSIIVKCSEQVIYSSEYFTGIIKKVLEVCGHDPELVNLFYCFPPNLESASLLTSKTKKYTADYFSANPNFKHLTFIGSKPVAQHVLKQAAEPITPCVVELGGKDCFIVLNSVKNLQSLSSIIMRGTFQSAGQNCIGIERIVVAESKYDELVNILNKRVGGLRLGSDIDDLVENVDMGAMISDNRFQQLEDMVQDAVSKGARLLHGGSRYQHPKYTMGHYFQPTLLVDVTPDMDIAQNEVFGPIMTIMKAKDNLDSIIEIANSAEFGLGGSVFGANYDDCVYVAERLTTGNVAINDFATFYVCQLPFGGCNGSGYAKFGGEEGLLGLCNAKSICYDTLPFVSTQIPPQIDYPIKDNKKAWEFVKALNTASYTMSPWTRVKEIFKLAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1H0</accession>
    <name>A0A1E5R1H0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g4320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1H0"/>
    <dbReference type="InParanoid" id="A0A1E5R1H0"/>
    <dbReference type="OrthoDB" id="4057307at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ80722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="76" mass="8348" checksum="80A6E703C0355928" modified="2017-01-18" version="1">MGLKDTFLKVKRWFSGEPIPGETYKQAPDVKDLPHIVDSSGALVAVSSGKRELSPVLSQKLNIQKSPKVSQIDDQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1I8</accession>
    <name>A0A1E5R1I8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lariat debranching enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1I8"/>
    <dbReference type="FunCoup" id="A0A1E5R1I8">
        <property type="interactions" value="683"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1I8"/>
    <dbReference type="InParanoid" id="A0A1E5R1I8"/>
    <dbReference type="OrthoDB" id="3972381at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008419">
        <property type="term" value="F:RNA lariat debranching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12849:SF0">
        <property type="entry name" value="LARIAT DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12849">
        <property type="entry name" value="RNA LARIAT DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="417" mass="47934" checksum="BBDFE08640F69776" modified="2017-01-18" version="1">MKLNVAVHGCVHGDLKKVYDLILSKSHTQKKVDLLIILGDVQTLRSAQDLVSISIPPKYLYNNNVSRITDFPKFVKDHYKIPIPTIIIGGNHENMKQFAELPHGGYIYPDLYYLGLKSVVTFKGLRIAGFSGITNLYDVYKQLPVVPRSSETSKTSNTLGNQQNQWWNKNKKTLYHVRFMDLVPLYLYAVCSDLPLDMVLSHDWPAVVTQHGNIEDLLKRKPYFRKEVLNGELGSPLYDPLLRVMKPKHWLSSHLHVKWGCEVVFPSVDVEKNKDEIDLFDDEEESLGSNFPSVTKFLALDKYLPSRPGQSFDYLEMDVCDDTTNLFEYDPVFVNILRFVNTHKTEIQRLVNPRCSFEENFANVRGFFDAHGEKSMLNKKNDLDYNIVGYEEDLSKQTTEFVSRFLYTNITSEASGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1J3</accession>
    <name>A0A1E5R1J3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nucleus export protein BRL1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3967</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1J3">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R1J3"/>
    <dbReference type="OrthoDB" id="3972387at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006998">
        <property type="term" value="P:nuclear envelope organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040202">
        <property type="entry name" value="Brl1/Brr6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018767">
        <property type="entry name" value="Brl1/Brr6_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28136:SF1">
        <property type="entry name" value="NUCLEUS EXPORT PROTEIN BRL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28136">
        <property type="entry name" value="NUCLEUS EXPORT PROTEIN BRR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10104">
        <property type="entry name" value="Brr6_like_C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01042">
        <property type="entry name" value="Brr6_like_C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="347"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="458"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Brl1/Brr6" evidence="3">
        <location>
            <begin position="351"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="510"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="540" mass="62105" checksum="06E89EA516F864BE" modified="2017-01-18" version="1">MENLVRIQLKEFKVPSDQEPTGDALRNNIIRHAENQTIHQRVRNYEFRAHDYTDPGVRWCRKKIETLNLWDDDSEEQKRLQSVSLMDVDPCVQEDAKMNDDXLSNSSEMEVDAEGLPGALEAEELIGNPQILPDELGFENEKSDQTDISLRKFFKVMVSPTELGVSMAQKGEENNNVEIQTPFESMQDEMISNSHSDTQDKTNTFETSPGRGKMDVEQDKNTECEFGNLPVNQDPVDKESVEIEAGKEESKSKNHTSFDPSKNSANSHQSIDDHAIENKNHLHAKKLQPYNLEINNHYYNYIYMPGGNNAPENQHQAYQDQSSLVHSPELQALPEPWSKNSHPHSKFVYLFTSYLQLFLNSLLYMGICSLVLMILRAFYHDLKTTWNQEVLKLHYESSVCKFQYFINKCSSQQSPALQAKCLQWGQCMDRDNDQMYVGKTASIAKLIGTMINEMVEPIGWKVVLLLATASYLWIFGLNLMFGFXRAKSYYSGGKRQKPLKDNFKERFSTQPDFAHRKGKNDDQSFPEGLDEPLQRKRIAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1S4</accession>
    <name>A0A1E5R1S4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">UPF0587 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g4299</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0587 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1S4">
        <property type="interactions" value="563"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R1S4"/>
    <dbReference type="OrthoDB" id="10248838at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008584">
        <property type="entry name" value="CXXC_Zn-binding_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12857">
        <property type="entry name" value="CXXC MOTIF CONTAINING ZINC BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12857:SF0">
        <property type="entry name" value="CXXC MOTIF CONTAINING ZINC BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05907">
        <property type="entry name" value="CXXC_Zn-b_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141678">
        <property type="entry name" value="MAL13P1.257-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="198" mass="22419" checksum="485525CF9CB821BB" modified="2017-01-18" version="1">MLYLIGTAILSENIKSFDFLDTEEQPLDYPFKIKCTSCHEVSASAHKFNSQDEVDRNNISTVNFSMKCKFCGSTCSVSFEKTKEKLYNSVGGDIXSEDEAVLEAKELVARQLKKRAENYPKLVAKTDKSANFAVFLKFDCRGCEVVEFVPDEDTIIEVSLYNGNVIENCLEDGELFDYDDKDEQEVSVTEVQWNIVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R200</accession>
    <name>A0A1E5R200_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcription factor STP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4144</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R200"/>
    <dbReference type="InParanoid" id="A0A1E5R200"/>
    <dbReference type="OrthoDB" id="9439903at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="4">
        <location>
            <begin position="268"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="73"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="441"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="576"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="514"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="460"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="5">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ80929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="842" mass="93478" checksum="EBFAFCF91BF8B03C" modified="2017-01-18" version="1">MSKKVEASSTVTNDNPSMLKKISTRIFGFFNKLVSTVVVGSTTNDGYTPLKNKKQAKTSSENSVVKDIQIDTXAXEQDVDSETQDXIRDTKTSKSEHRAPKIENDEEDFIPPQSGSLFPNTIVVDHSLNNNATAIPCSLDMSLHNNEPFNMNMHNMNMQNTNMHNMNMTALPQMNNTENIQHVQHMQNFQNAQSMNNMPNMHSNVLMIPPMQMPVNTPMNMNSLNTPNSSPSDSLLKNKIQQQPSNELVSMNNTKENEEEQPHQPHLYVCHYCDAQFTVRGYLTRHIKKHAVEKAYHCPFFDVHLDPSFRCHPTGGFSRRDTYKTHLKTKHFIIPKGTSLKDRLKTPGNCGMCGEAFENSETWCEKHIATGSCCKLPEGFTSLPQKSSQHKLTQYTLKLIKSENGVARYISSKESIVEPKVFANREAVDIMAELAQESLFSPHSSSSSAETPMNHPSVESNGSSSSXSSSGGNNHGNLNGSTGPSLSQNHSYTPIIEKLSDNRIVIHSENIEGIKEIKNKKGKKKNALKKLASNLANANKKGKVGAKNAKIGRKPGVGPNFYGSVTSPPMGIHNANTVAGPHSQITPRSDDQSSIEDAGLEPIKSNSSQSSNESLRAFLNATQQLPLPVTHQQQYNGVPLNGASLTTSQPHFHASNLNNQQGRDVAQQLAVTKENFQHIQNLIPSVSFYNHPNAGATASTDYQNTADDYLSLDTEQQAAPYSSLYQQPVRTLPQHQHINKYVSPVPSGSTPVQYHQQQQQQYQSLDQSQTYGNSTHAVYDTGLHAFNADSYANYNEAPISKALISSVNDQLALSEQQVRETEQYRKFYEFTFQKPESQVTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R237</accession>
    <name>A0A1E5R237_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R237"/>
    <dbReference type="InParanoid" id="A0A1E5R237"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="127" mass="14196" checksum="9C5E40CE507EE2BA" modified="2017-01-18" version="1">MSGQRPYKNNNTNQDKTNPFLKNEKREYSEKKDSNSKVYSNSGNGPQIQGSLMNADNDGLPTYDESMQAAGQATQSDYGAQYIQTRLEATRPPTNNMNAPYVHFPHPKSNTNFPGGKTTTYNNVGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R245</accession>
    <name>A0A1E5R245_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nucleolar complex-associated protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g4034</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Required for synthesis of 60S ribosomal subunits and the transport of pre-ribosomes from the nucleoplasm to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
            <location evidence="1 5">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R245">
        <property type="interactions" value="1143"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R245"/>
    <dbReference type="InParanoid" id="A0A1E5R245"/>
    <dbReference type="OrthoDB" id="10263597at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011501">
        <property type="entry name" value="Noc3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016903">
        <property type="entry name" value="Nucleolar_cplx-assoc_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14428">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14428:SF5">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07540">
        <property type="entry name" value="NOC3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028977">
        <property type="entry name" value="Nucleolar_complex_p3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Nucleolar complex-associated protein 3 N-terminal" evidence="9">
        <location>
            <begin position="165"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="CCAAT-binding factor" evidence="8">
        <location>
            <begin position="498"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="45"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="117"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="391"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="117"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="138"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR028977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ80964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="687" mass="78696" checksum="4D05339E5CB62305" modified="2017-01-18" version="1">MGSKKRSQQIIQDRAAKRRKEADSLLSPGAFSLEAGLNAGDKEFVVNEEAPTATTRRSKNATWDDEEQEYETKPRTLKYSQDDEMVEGLPIKINGKIERNIHKKQLSLAQKEKLAQKEKEEQERAKVEEQAKIQEQVKQQEKEREQDKEKEEEEEEDTEERILALKEDIANLVEQIMEDPEENIGALTRLRKMAESKNPNTCKFSMLALIPVFRSIVPGYRIRPLSEAERKEKVTKEVARLRRFEENLVANYIGYVDILTKLTKTPNNDHIIKVQLGNLAANAAAELAGNFFEFNYRKELFTLVIHRVCKPNPSVDPAYPKLIKTLETLLYDDDEGNVTVEILRILTKTLKVRNYMVDEAVLNILLSMDILDDYDPYVETQEKVKIKKKDRVHLSKKQRKARKEMKQIDEEMRKAELAVSAEQREKNQAEILKLTLALYLQILKANVSKLIGSVLEGLAKFGHMSNFDLLGDFLEVMKELIKESDLDTLSPLEVRKVLLCIVTAFSLVGSHTHMKFSMDLSYFVDSLYAILPYLSLDADVEFSHKTLRLADPLNNELMKPSINVSTKAELLLKALDHIFFRSKSGTRQRASAFTKRLYMCALHTPEKTSVAIVKFMDKLMNKYPEVAGLYSTDERIGNGNFNMTTNEVERSNPDAATLWETVLLTKHYSPIVNKGVKGLXHKSRGQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R246</accession>
    <name>A0A1E5R246_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UBX domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R246">
        <property type="interactions" value="146"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R246"/>
    <dbReference type="InParanoid" id="A0A1E5R246"/>
    <dbReference type="OrthoDB" id="1026733at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF1">
        <property type="entry name" value="FAS-ASSOCIATED FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="71"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="667" mass="76538" checksum="40EB6192DAA49E77" modified="2017-01-18" version="1">MSGTPTDSELCGNLNDFQQQTLHEFRGITAFADEDLGKVIKLLEISHWELLGAIAKYFDEDWKQLIVNHEQQQQQQQQQQQQRTRSPPEPDVPARPTLLRDMMDQNSILLPDYASFRPRLPVVTRLDDNYRSFLRPGLPVKFNHKTKSXSYGRSGLISESTTQTQTGLLLNRLSLILLFIPQTLFSLGWKLISFVWTMICYGVGLRNESVVDNTXAARNGEVDQKKTGKYVNKKPIGDFVCRTVDDPQFSGVLKNKYTDDARMLEKLEKLHFSSFNEVYDDCRDNYKFMVLIMLGGVLDTADQVTEQKNDSSLSSPSSSSSLVDEKXDVVLREKHGVTKSDSAAFLENVFLDDQIVNFFQENKEEYNFYMCDVENQFEGWKVSRDLKIKQTPSIYVIANVTNSNYPSPTGMSVLGGFVRFEKPGLMLRCLEQFASKFNNELISSRADKKEIELTRFLKEQQDMQYQESLQMDKDKNDKKLVDNFQKIYLLHKCSELLTTKGQAVESGDSKKDCTIQVRTSTGTRFLLSYYGDKSYLSDVYEELSLYLYLGIENGNDIKRLSVLREKITDHVEQVLNDDSNMLFKDDSIEELDEETIETLIGEEYDRFDKIAGLSSTKKQEIISSMLDSYDFTLVTTFPKKTILNDKSTLLESNADIYPKGNLLLEFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2F5</accession>
    <name>A0A1E5R2F5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Hexose transporter 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g4132</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2F5"/>
    <dbReference type="STRING" id="56408.A0A1E5R2F5"/>
    <dbReference type="InParanoid" id="A0A1E5R2F5"/>
    <dbReference type="OrthoDB" id="4095185at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015149">
        <property type="term" value="F:hexose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF75">
        <property type="entry name" value="GALACTOSE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="71"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ81064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="151" mass="15866" checksum="50CC7CBC9276045E" modified="2017-01-18" version="1" fragment="single">MSESQTPEVLGSSSNTPADQASQLSTPSNNHSHDASEIKHDGFGEPEFPPEDEPALKNTKGLSAYFGVVSLCLCVAFGGFVFGYDTGSISGFVAMSGFKERYGSHNSSGYYLSNVRTGLMVSIFNIGCAIGGLILGKLGDMVGRKLALMVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2G6</accession>
    <name>A0A1E5R2G6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">V-type proton ATPase subunit c</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g3906</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Proton-conducting pore forming of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Proton-conducting pore forming subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10 11">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d, e, f and VOA1). The decameric c-ring forms the proton-conducting pore, and is composed of eight proteolipid subunits c, one subunit c' and one subunit c''.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2G6"/>
    <dbReference type="FunCoup" id="A0A1E5R2G6">
        <property type="interactions" value="235"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2G6"/>
    <dbReference type="InParanoid" id="A0A1E5R2G6"/>
    <dbReference type="OrthoDB" id="10264021at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18177">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6F_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18178">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6F_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.610:FF:000002">
        <property type="entry name" value="V-type proton ATPase proteolipid subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000245">
        <property type="entry name" value="ATPase_proteolipid_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10263">
        <property type="entry name" value="V-TYPE PROTON ATPASE PROTEOLIPID SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00122">
        <property type="entry name" value="VACATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="142"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="179"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="12">
        <location>
            <begin position="61"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="12">
        <location>
            <begin position="145"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ81091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="212" mass="22025" checksum="B28B6D29AE58B301" modified="2017-01-18" version="1">MSSKDSVSLGLPVPTFSGTLKYTTLFVVVVYVGYKLFTGHGEDINIGLFLAKASPYFWANLGIAACIGLSVFGAAWGIFITGSSVIGAGVRAPRITTKNLISIIFCEVVAIYGLIMSIVFSSKITVASPETMFSHSNMYTGYSLFWAGLTVGACNLICGVSVGITGATAAVSDAADSALFVKILVIEIFGSILGLLGLIVGLLMGGKAVEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2L5</accession>
    <name>A0A1E5R2L5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">mRNA-binding protein PUF3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3850</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2L5">
        <property type="interactions" value="264"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2L5"/>
    <dbReference type="InParanoid" id="A0A1E5R2L5"/>
    <dbReference type="OrthoDB" id="668540at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07920">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033712">
        <property type="entry name" value="Pumilio_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF12">
        <property type="entry name" value="MATERNAL PROTEIN PUMILIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="4">
        <location>
            <begin position="579"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="600"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="636"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="672"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="708"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="744"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="780"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="822"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="870"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="145"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="353"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="409"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="504"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="174"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="365"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="436"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="530"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="548"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ81124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="947" mass="103118" checksum="385BD5B824ECF122" modified="2017-01-18" version="1">MSFSDPWSDNNVPSMNAELESVISSLSQMSNXQTNTNQYRASQQIGAHRRSSMTSNSDMGSDILVPHGTAQSLASNIEYQNQFQQGFAKEKTTXSGNNAGLHVDNSPILRRTTLSVGGYSLNGGSSNQGPSAFFGTNGGFLAGNNPNGSPVNTNANASASANASASANNNNNNTLKQTSNINGNTNNLANPNRMAGYAASIAVAQQNANFGSNYFNSSQNNFNNNPTGGPGTNGNVGGGSFFETFGKQLVEATKEVEGGTNLVADDELTVQQFVTSSSRSTSLQVDAYTAHTDSRQNSNFLGEEVGVPEAPLSTSRWNPETARSFYPSAPMGPGVHSGFPFNGLFHPLGLPPHHHHHHHFTHNPSSAGASTTIEQSTEKGAKIEYAKNPETPGIPAPIASPPHFFMNPYLAYGATPPPPPAQNXKDKSNVDEPSGETLQTADPSNSEFVPAYFGAPDPNAFEENGKMNPYQYGQAPFAQGYSYGGSYFNPMSMSLPLGSAPAPTGFAPQKAGQGNGKHNKGNPYLKNRNGKGSAASKSGKAAASVSALHQSPQNTEPAHSTKSGAGSKHNGRRRKEPVYRSPKLDEFVKSSKDKKYELGELYGHAYEFCKDQIGSRFIQDKLPTASDTDKEMIFSEIRDHALELMDDVFGNYVIQLFIELGSDIQREALVQSILGHVLDLTQSQYGCRIIQKSLMYVTLEQKILIVDELKDRVYDCIKDQNGNHVIQKAIENIPLEKLPYILESLKGQIYHFSTHPYGCRVVQRLLEFGSEGDRKLVLQELKNFFPYLIQNEFGNYVVQYILKSSAESSTQDMLEVKQDIVDLVCDRIVDFSCHKYASNVVEQILFLGNESQKKQLFDVIIPKSEKDAMELKDSELIAIMMKDQFANYVIQKLVKVTSGENKRLLIISINSYLDQVKATNNNANGGRKQKHLASVEKLAALVSNLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2M5</accession>
    <name>A0A1E5R2M5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g4263</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2M5"/>
    <dbReference type="FunCoup" id="A0A1E5R2M5">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R2M5"/>
    <dbReference type="OrthoDB" id="3972963at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ81157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="92" mass="10881" checksum="9394D4C2ABAC0489" modified="2017-01-18" version="1">MSFKVWNSLSLLERQRFSTKFVQNYKKLYPGSKTNVSLNAMIVDMATFRDVPAVFQVFYNDISKLHMSETLNRNTYGRFSHPSFVELLYKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2P1</accession>
    <name>A0A1E5R2P1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sorting nexin-4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Autophagy-related protein 24</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4029</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sorting nexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2P1"/>
    <dbReference type="FunCoup" id="A0A1E5R2P1">
        <property type="interactions" value="529"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2P1"/>
    <dbReference type="InParanoid" id="A0A1E5R2P1"/>
    <dbReference type="OrthoDB" id="205639at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45949">
        <property type="entry name" value="SORTING NEXIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45949:SF2">
        <property type="entry name" value="SORTING NEXIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="12">
        <location>
            <begin position="40"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="71"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="421"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ81148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="493" mass="56830" checksum="677C3C1CE47B2B31" modified="2017-01-18" version="1">MANISGRGFSDNEELLNDANHDTKISGVTAGLNKTAYEDFFLDVMVSDPQRQRDEHAAFVNYQVSTKTDKKSYHHVYRESNSTNTDTLQKKKSNSSSKAKQDKNEILVVHRRYSDFLLLHNILMNDYPCSIIPPIPDKKVLQYFAGDRFSPSFTQKRCHSLQNFLQKLALHPVYKNSKILETFLTNSTEWEIYRRTINISNAEGSSGKSHAGNGGNSSNKEDVSDYFINAFKHVHHQSDEFIEIKEKSEKLDYNIKKIDKVTHRVVKLQDGLIEDYGKFSKGIDNFRELLMKNEFSSGCADNNSADENMSISVDHSKLVESLAVLNSGLVTMQYALKDLTKYYDFEYLTDLRDMEHYIDNLKHLVKLKDQKQIDYEELNDYLTKSITEKNNLISGYTGNFFTNKLEEIAGINQEQSRREKISKLDGKIETLTEEVETAKKTSLDFEKQTLKEIEIFENIKTQSLKKSLTGLADNQIEFYEKLYETWSKVEEGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2R2</accession>
    <name>A0A1E5R2R2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Central kinetochore subunit MCM21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3977</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CENP-O/MCM21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2R2">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2R2"/>
    <dbReference type="InParanoid" id="A0A1E5R2R2"/>
    <dbReference type="OrthoDB" id="10050372at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018464">
        <property type="entry name" value="CENP-O"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09496">
        <property type="entry name" value="CENP-O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="9"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="9">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ81168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="335" mass="38879" checksum="92B4DFC5E3294F2B" modified="2017-01-18" version="1">MSDQDLEXCKQDIAALNQELQRLRDEKMSLQARIDSSAKSKDTLQQLLQKHAKEKEEFDDFLLKRPHSQLASSHNXQLXLKKQKHQRSRGPSARHQTPEYLPTDIFDPSIRSLLDTSVLYSPSKKALQSNTPEYISALSQQDLESLQRYTQVEKCYRVMLGLTCFPLRDPLDNKTTSMQRDMVGLRFDLFDQKTRSFKVPYYILFKYASSTQNWKIFKSTVPENVVGGLELLQSSEIPILSSLEDIYLFGKIVYNILKAWVKRNNYIKTLFKKNNVKNFFLDDSARLIRYDHNGHDIELEVDEHDSVVGTNSNIYEYNFFPLKEPNRGSLSPSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2X2</accession>
    <name>A0A1E5R2X2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Thioredoxin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4289</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2X2"/>
    <dbReference type="STRING" id="56408.A0A1E5R2X2"/>
    <dbReference type="InParanoid" id="A0A1E5R2X2"/>
    <dbReference type="OrthoDB" id="10263751at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000245">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01068">
        <property type="entry name" value="thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46115">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="16"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="139" mass="15550" checksum="BAC6A116200B21C7" modified="2017-01-18" version="1">MMLGSLTRCRTAVPSQMFIRAATRFNSTTSSNVQLIESLNDFKKTVVDKSNEKLKMVDFYATWCGPCKAISPMVEKWADEYKESADFYKIDVDQAPEVAGFCGVSAMPTFLFVKDGKIVSKVVGANPKNIKRDLDLFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4L7</accession>
    <name>A0A1E5R4L7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Proteasome subunit alpha type-4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3663</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4L7">
        <property type="interactions" value="847"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4L7"/>
    <dbReference type="InParanoid" id="A0A1E5R4L7"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03755">
        <property type="entry name" value="proteasome_alpha_type_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000004">
        <property type="entry name" value="Proteasome subunit alpha type-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 7 9" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="8">
        <location>
            <begin position="4"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ81855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="250" mass="27651" checksum="C05B5D6ED47140EB" modified="2017-01-18" version="1">MSGYDRAISIFSPDGHIFQVDYAAEAVKRGTCCVGIKGADCVVLGCERRSTLKLQDPRITPSKISKIDNHVMLTFSGLNADSRILIDKARVEAQSHRLTLEDPVNIEYLARYIANVQQKYTQSGGVRPFGVSTLVAGFDPNDKTPKLYQTEPSGVYSAWFAQAIGRNSKTVREFLEKNYDKDSLPANESEAVLITIKALLEVVQTGAKNIEIAVVKANNDITILSSKKIDELVTEIENEKTKEKVEXDNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4U5</accession>
    <name>A0A1E5R4U5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">tRNA:m(4)X modification enzyme TRM13</fullName>
            <ecNumber evidence="3 15">2.1.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g3761</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 15">tRNA methylase which 2'-O-methylates cytidine(4) in tRNA(Pro) and tRNA(Gly)(GCC), and adenosine(4) in tRNA(His).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>adenosine(4) in tRNA(His) + S-adenosyl-L-methionine = 2'-O-methyladenosine(4) in tRNA(His) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43196"/>
            <dbReference type="Rhea" id="RHEA-COMP:10401"/>
            <dbReference type="Rhea" id="RHEA-COMP:10402"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74477"/>
            <dbReference type="EC" id="2.1.1.225"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>cytidine(4) in tRNA(Gly)(GCC) + S-adenosyl-L-methionine = 2'-O-methylcytidine(4) in tRNA(Gly)(GCC) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43192"/>
            <dbReference type="Rhea" id="RHEA-COMP:10399"/>
            <dbReference type="Rhea" id="RHEA-COMP:10400"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.225"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 15">
            <text>cytidine(4) in tRNA(Pro) + S-adenosyl-L-methionine = 2'-O-methylcytidine(4) in tRNA(Pro) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32767"/>
            <dbReference type="Rhea" id="RHEA-COMP:10397"/>
            <dbReference type="Rhea" id="RHEA-COMP:10398"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.225"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the methyltransferase TRM13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.225" evidence="3 15"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4U5">
        <property type="interactions" value="467"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4U5"/>
    <dbReference type="InParanoid" id="A0A1E5R4U5"/>
    <dbReference type="OrthoDB" id="258806at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106050">
        <property type="term" value="F:tRNA 2'-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007871">
        <property type="entry name" value="Methyltransferase_TRM13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039044">
        <property type="entry name" value="Trm13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022776">
        <property type="entry name" value="TRM13/UPF0224_CHHC_Znf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021721">
        <property type="entry name" value="Znf_CCCH-type_TRM13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12998">
        <property type="entry name" value="TRNA:M(4)X MODIFICATION ENZYME TRM13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12998:SF0">
        <property type="entry name" value="TRNA:M(4)X MODIFICATION ENZYME TRM13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05206">
        <property type="entry name" value="TRM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11722">
        <property type="entry name" value="zf-TRM13_CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05253">
        <property type="entry name" value="zf-U11-48K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51800">
        <property type="entry name" value="ZF_CHHC_U11_48K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 15" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="11 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHHC U11-48K-type" evidence="16">
        <location>
            <begin position="69"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ81935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="463" mass="52645" checksum="48B8F447DD6FCF10" modified="2017-01-18" version="1">MDTHVDKKPKIXETTVDPARCEFHIVKKNRQCGMLKSKQSTQYCTEHLLLAKRNEGAQLRNNNTKKGNRVPCPLDNNHTVWEIDLKKHLKKCTKGKSNNMNAGKPWFVPDVNCGQSESSSGESKSTQELILQVIPVLQKLQELNMFDGDNIPRKEILKDQHVQDHRVEQLLGNQSRHAVQQSSLIQHIRKSLNKNSNKTGNPLFVEFGCGRAELSRYLNLSLSLDHESDKKIADESKPSMGNXLSFLLIDRGANRLKFDHKFAEDCHPLPGPVVKREKADIKDIDLNALIXCDFQXHDELNVVSVSKHLCGVATDLTLRCLEKSMTNAENNKKYSVCIAMCCRHVCNFDDYVNPLYISSLLEKHDSAKNLKASEFFYSLTKICSWATCGRRPDVDIHTRATHFTKLSIEEREQLGMISRRIIDTGRLEYIQQCDVWKQHTSSSAKLVQYCDLSTSLENVALVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4Y6</accession>
    <name>A0A1E5R4Y6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycerol-3-phosphate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="5">1.1.5.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g3562</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a quinone + sn-glycerol 3-phosphate = dihydroxyacetone phosphate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:18977"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.1.5.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Polyol metabolism; glycerol degradation via glycerol kinase pathway; glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.5.3" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4Y6">
        <property type="interactions" value="646"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4Y6"/>
    <dbReference type="InParanoid" id="A0A1E5R4Y6"/>
    <dbReference type="OrthoDB" id="264015at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004368">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.870:FF:000005">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.9.10:FF:000037">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.870">
        <property type="entry name" value="Alpha-glycerophosphate oxidase, cap domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031656">
        <property type="entry name" value="DAO_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038299">
        <property type="entry name" value="DAO_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000447">
        <property type="entry name" value="G3P_DH_FAD-dep"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11985">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11985:SF15">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16901">
        <property type="entry name" value="DAO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01001">
        <property type="entry name" value="FADG3PDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="14">
        <location>
            <begin position="104"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="14">
        <location>
            <begin position="382"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-glycerophosphate oxidase C-terminal" evidence="15">
        <location>
            <begin position="558"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="484"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="485"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF16901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ81928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="720" mass="80322" checksum="B8F197271D941C35" modified="2017-01-18" version="1">MATRLGSIRKYLTSRNVLLTATIGTLVSTTYLYNNHTKHDDLDKLKSDNNYDNNNDNNTNPFMDNSISKTYSRLQKNKKKLPLPSRQILLEKLKNNIENDIINDVLIIGGGATGTASAIDAITRDPNLKVVLVEMNDFSSGTSSKSTKLIHGGVRYLEKAVFQLSKSQLDLVIEALXERKHMISTAPHLCKILPIMIPAYNIWQVPYYYVGAKLYDVFSGWSNLKSSYLLSSKNALDICPMLEKDSLKAGIVYHDGSFNDSRMNVSLATTAIEKGATILNYMEVLQLLKNEKTGKVEGCIAKDKETGETYKIKSKTTVNATGPFSDRILNMDNNKDGLPGDKDLLQKINQNNDNENENKNDNNNNYDFSSNQIENQVAVVDPKMVIPSAGVHLILPSYYCPANMGLLDAKTSDGRVMFFLPWQNKVIAGTTDVPLKNVPQNPTATEADIQDILKELQHYIKFPVRREDVLSAWCGVRPLAKDPRVSNTIDPGTSNSSKKQPKNKTEGLVRSHFLFTSDSGLITIAAGKWTTAYSMAKDTIDEVVKHLYGGTDASARPCKTGTLKLTGAEDYSPNVSALLSQEYGISTQLATHLADNYGTNASVICEMFKKNPRHALPLQFGNPNLTLQDVDFKTFEYPYTIAELQYAMDYEYARTPLDFLLRRTRFGFLDTAKALSAAEGTVELMGEKLAWDKEFKKRQLEYTTAYLKTFNYHTSXPSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R539</accession>
    <name>A0A1E5R539_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ribosomal RNA-processing protein 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3641</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R539"/>
    <dbReference type="FunCoup" id="A0A1E5R539">
        <property type="interactions" value="1084"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R539"/>
    <dbReference type="InParanoid" id="A0A1E5R539"/>
    <dbReference type="OrthoDB" id="189968at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000644">
        <property type="entry name" value="Rrp9p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039241">
        <property type="entry name" value="Rrp9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19865">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19865:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="238"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="324"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="42"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ82011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="574" mass="65037" checksum="5055AFF496B87496" modified="2017-01-18" version="1">MAITDRKRKRSSKPSRAEKSDSAPARSAVNDEDITDPSSGEESADEQEDSVEDVEDELESDDEFEGENPADKRRRLAKQYLSNLQQEANKIVTENVTDERGQPNQEIDDYNNFDAADLEREIIQSRLKQDVAEQEGKVYKFVADHLSLKNAKSTFTRVGQKNLTGLSCYQPMAIDNTFSIEDTNKRQRLYAYTVSKDLQLTKYDITDFNKRPQVMKSVKGGKEFAPTEPLKTEYNNNSEGHYDEILSVAACPNGRYVVTGGRDRKLIVWSTEALAPMKVLPTKDRRGQVMGLAFRKNSDQLYAACADYKVRTYAINQFAQLEILYGHQDHVVDVSALAMERCVTVGAGDKTVMLWKIADESRLTFRGGNEDPDRLKKKWLKNNEGKTEKDCPHFYTENYIDCCSMIDDSHFVTGSDNGSISLWSTSKKKPIFTMRTAHGILPKIKGNQISAETNKEKRNLQLQGQNLTKPYWITSIYAIPYSNVFVSGSFNGSLKLWKLSEDLREFELLKEFTNAKGVITKIQVVTSGPPNKETYRILCTVAKEHRLGRWISNVPKARNGLYSVVIEQNVSKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R541</accession>
    <name>A0A1E5R541_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">DNA polymerase alpha-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3642</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R541">
        <property type="interactions" value="381"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R541"/>
    <dbReference type="InParanoid" id="A0A1E5R541"/>
    <dbReference type="OrthoDB" id="427368at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022100">
        <property type="entry name" value="WDHD1/CFT4_beta-prop_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048591">
        <property type="entry name" value="WDHD1/CFT4_hel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19932">
        <property type="entry name" value="WD REPEAT AND HMG-BOX DNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19932:SF10">
        <property type="entry name" value="WD REPEAT AND HMG-BOX DNA-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20946">
        <property type="entry name" value="Ctf4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12341">
        <property type="entry name" value="Mcl1_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="WDHD1/CFT4 second beta-propeller" evidence="7">
        <location>
            <begin position="526"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="domain" description="WDHD1/CFT4 helical bundle" evidence="8">
        <location>
            <begin position="866"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="472"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="944"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="476"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="9">
        <location>
            <position position="840"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="9">
        <location>
            <position position="886"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ82014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="972" mass="109653" checksum="FA9E36A26F706CE3" modified="2017-01-18" version="1">MATNQNPEVVEKEVFSLRGKTFVNISLNDVLLTINEKSICKIFELNNSDKDEPEVVDTCESIAAFTPIFKSPITNKWAFCLADKIQDNVYLYTHSENIAMAQPKTIFRSFLDIRDICIVHNGKILAVASDDLEVKLLFLEDIIRDNSGDNGSVSPSGNTKRSKSIKVSKPVVNISFSHILNILAISCNDGSISLWSLSSDSPRLVYGGDDGEEQLSNYVTPNYTLGLDEDEDDDEDEPTDGITSTTPIADKLDEVSSSRVAFGDNNGVFACCVNLTISTYAISDVKKKVKSFTHDNKIKDFKYCGNDNYLCLIDYSDRLLLWHIASETLVFNKLITKDDNKLTNLAWKKSTTNSNNFNVYCGTKNGNVVFIENILSPNNVSLLSTKAATKSDSGNYGLFLNDSDNEDVDDMEEVTKRKQNLNNTISMGNDLFNDEAEEDNIGIKEGHGSASGKKRAHFDDYDDLDEELKNLNNDSFADDLDENDEEENMXDDGNDDSDDGGSLFNDYNGRPHKRVHFGPTSESLKPWSSAGTEFHLSDRRYLTMNSIGYAWTVKSSNTQSVTVSFFDRSTYREYYFEDYNMSDVCSLGEKGILLANSKKGEIAFRSHSTDSNKWSKTIPLQKKELITSVALTNKKCFLGTSLGNFYELNVFGYIINIERLSPVAAMVAYHNRVFIVHRSASVSFGNELLTFSLFDDQHYYQREFILPIKDFMKNIFFTQYGDPAIFTKDNTLLLLSKWRDSNKSRWVPVLDVGFEIWRLSGGNQSSNDIHCWPLGIAGNYNNTLNYIMLKGSKRYPEFPLPLPTDMEIKIPIFNKPLFEEVKKQKDLEDKIRKEEGNGADGDEIEDDDLASIAYGTKELEIPQEVNAEEEFLRSKISXTMLQDTLDNDGEIYGDEQTTLDQLNTIYDKSLLRMFAIACSDSDIKKAYSLVIELKQDRAFGAATKIAERAELIQLAEKINQLREARSEQQMNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R573</accession>
    <name>A0A1E5R573_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAD synthase</fullName>
            <ecNumber evidence="2">2.7.7.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">FAD pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">FMN adenylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g3710</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>FMN + ATP + H(+) = FAD + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17237"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.7.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.2" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R573">
        <property type="interactions" value="150"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R573"/>
    <dbReference type="InParanoid" id="A0A1E5R573"/>
    <dbReference type="OrthoDB" id="270728at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23293:SF9">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23293">
        <property type="entry name" value="FAD SYNTHETASE-RELATED FMN ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoadenosine phosphosulphate reductase" evidence="13">
        <location>
            <begin position="59"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ82018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="344" mass="40916" checksum="F2188FD2667DA089" modified="2017-01-18" version="1">MKQEILAHCYHIVETYLSISTNVTSELPIIHETQQRIQETKSVFLERILPFWNPVLNNISISYNGGKDCQVLLIIYLACLYEFFQNRFSMQQAYLDNFQVNGVYIKQYETFHILENFVLNSAQYYDLNMYTTQVGQNMKDSLREYLGHLNLKDYNQTREDENERDWDLDGVEMGNLQKIKSTHERQEIQKDHHGVIIGIRRSDPYALDLKEIQRTDADWPCFTRLQPLLEWKLQNVWSFLLFSNDXFCKLYELGYTSIGGIKTTLKNPKLNKKDNKNLWNHFHWELEHAYVTETHDTHAPFLEVYKQEDTLVQSDIDHDLVCNDTSYYPGWYLISDKQERDGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R588</accession>
    <name>A0A1E5R588_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1</fullName>
            <shortName evidence="8">Oligosaccharyl transferase subunit WBP1</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3676</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the DDOST 48 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R588"/>
    <dbReference type="FunCoup" id="A0A1E5R588">
        <property type="interactions" value="1041"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R588"/>
    <dbReference type="InParanoid" id="A0A1E5R588"/>
    <dbReference type="OrthoDB" id="29105at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005013">
        <property type="entry name" value="DDOST_48_kDa_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055459">
        <property type="entry name" value="OST48_MD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055457">
        <property type="entry name" value="OST48_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10830">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE 48 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10830:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE 48 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23358">
        <property type="entry name" value="OST48_MD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03345">
        <property type="entry name" value="OST48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009028097" description="Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1" evidence="8">
        <location>
            <begin position="22"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="401"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="OST48 N-terminal" evidence="9">
        <location>
            <begin position="28"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="OST48 middle" evidence="10">
        <location>
            <begin position="280"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ82066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="441" mass="50171" checksum="F54708F4A6059FFC" modified="2017-01-18" version="1">MLLYNLITLLCVCLMNDVCKALSVHPLKTLVVFDSRLTSLQDHSEFLQSLENRDHELVFEQVDHEDFDPQFYFLSEDEHSNLYDNMIIFPIKTKRGSSSLTSSKLLKFYENGGDVLAITSPVGVTDPVRLFLNQLSIYPAPKGYILRDYFNASDEKKNTFAIAGSDSLNDYVFNSSSDAELVYSKGSSALLGSSDLLVPILKAPSTSLNKNVNDEEWTIGSQGYLIAAFQNLKNARMTWVGSDKFFANGLYESNNKAFIEELTKWTFREKSVIKVNGAKHYHADKTSYEELPYKTTDEVVYEILLSEFKDNKWIPYEGTDVQFELKMIDPYYRLTMNAEENVGESKKFTTGVFKLPDHHGVFTFQVDYKRPGLTYVESKSTHAIRNLAHTEYPRSWEITNARVYLCSIFAVIGLWLVFVVAFIATSKKVVNKPTAASKKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5G8</accession>
    <name>A0A1E5R5G8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Restriction of telomere capping protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3820</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5G8">
        <property type="interactions" value="128"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5G8"/>
    <dbReference type="InParanoid" id="A0A1E5R5G8"/>
    <dbReference type="OrthoDB" id="3972670at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061700">
        <property type="term" value="C:GATOR2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016239">
        <property type="term" value="P:positive regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037590">
        <property type="entry name" value="WDR24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46200">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46200:SF1">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="266"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="432"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="333"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="857"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="901"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1070"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1164"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="81"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="113"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="901"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1100"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1111"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1141"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1164"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1190"/>
            <end position="1199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ82146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1414" mass="156448" checksum="BB8DC4391616DFA1" modified="2017-01-18" version="1">MSSHKRDKDLGNNNENVMKRMYGSSAVSGHQSLASPTSQTLPPTYNHSNSKHTKINTKQSAGGPGFFDXINGSYKHHPTYSNASSPKSHSPLSSFQEKGSYGPSGSSYNGNXLNQLSRHSSISGGRSKYYNSKGLRYSLRAQKEVSSIDKINDPLANTLVVAGKSHLGLYRFDPHHYGSIYLVRDVFQSSNNAQSNHGSFSFGKSSSLTSSSLNANKKKPSGMLSTICDVKAGFNNYSSYIATTSTSTAVSLYDVKRADTPLVGKLTEHTRSINSFDFHMGAQQNNMFITGGQDGTVKLWDLRKTGSSTRTNKSDISINTMSDSVRDVKWCPVWGGNDSQNQQGHGYRGNQDHTSNSSINEFGMSTHSSFSHSLSNISNIGNSNQSFDNKASTSSISSTASSNNGNYFASIHDSGNLLKYDLRNYSQPQKKINAHSGPGLCMHWHPNGEYIITGGRDGKLCLWFVGEGKPTFSPEQVMNVGQPVSKLKFQPTLKNSSSNAFIDSKFAMSPLGDDANVSIYSLANRYIPKNVLVTENSALGFVWYDENMVFSIDKGNIISGWDISKEADQLSNMSKNSLKWRDIEGDGLVFLSQKHSSSKSFEAPRSASTISHVTAPHTMKTSHTPNIIPKNKHRLSTSTVSGLLNSKSNSVANNLVGSNISTSPKQIMRSVPDIQHLARSPTPPSPQPHHLSKAALLKTFTQSTLKRAHSSFPNTQYSRPSTPIPFAQPMGIGSSTSPVLTAPHGHNPAFGSVSSSTSSAITNEETALWNEYQSPKVLTLDLPFIFNIIRTKNLNNFPTNAEEATKLSSTLKESPTEVFKFLARELKFSTQKEIDDKLQLQYLQEKKVQEQNGLKMLKSEGLEHTDEDGQEKEEDKDRKNLMKRLGISNTWSLADLEAKNEAKKLSTEKNTKATTKDNSSNEHTLESNENDYIDSLKIESNRKSSTNPVITFADMSNPDKNGNPTTKTHPHHNKHLAALKEKKAQQNLDNKKKRDLVMKNLMELITIADHNAETHLSIEDMASFKIWIMIRDSLLWKVKKLAARAEEGSSMLEKIDSYLTKNRKGYGFEFDEQINSNEQTAPRDRRNTNNIEGQGVSFGDESKMEEENFSRESSFSGSLNSHEGSFEYEYGQNMPSRKNSKSASDMGSSKSILEGIREKLNNASKSVEDEHALSDDYEEEGVGVDGKHFSSGTKPAQITQRRHSRTSFIDTMMNSLRSGKIDIDNEIQHRKNSMNPLLQRSPTFSIMPHSPQSVGSSLLTKEIKGLSEPTVGSSSAISENITCTNSVSVSEDKMNTFVEPWAPEILIKKIYSQAVDNGNVLLSMSILLLFQDHFSITTKDVVKIAVDEFMKILHKYELFEISAELLKYCPWGEEIAGEDEASTGQSAICLYCDKCHKLLVNEAMQEIKHVMCSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R6B0</accession>
    <name>A0A1E5R6B0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ankyrin repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3679</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R6B0">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R6B0"/>
    <dbReference type="InParanoid" id="A0A1E5R6B0"/>
    <dbReference type="OrthoDB" id="684045at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044515">
        <property type="entry name" value="ABTB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46231">
        <property type="entry name" value="ANKYRIN REPEAT AND BTB/POZ DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46231:SF1">
        <property type="entry name" value="ANKYRIN REPEAT AND BTB_POZ DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="46"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="127"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="286"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ82073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="534" mass="61812" checksum="B7C9EC6733D4B946" modified="2017-01-18" version="1">MQVKTELVRVHDEKFEQLCYACRIGDLENVDRLIAAGVALNGVDAFDNSPLFLASLCGHEEIVKLLLKRGSVCDRDRHEGARCIYGALTDSIRNILLDYDISKAVDLSQPFAMHISSLIRKETAIYQDIKMKFDLSHETLYLHKFILASRSRYFNKKFSSSWEEREEFDVYGTXFIEELKLLINFMYLVPILHLITASQIKNMIVLSKKFQFVELQXFLQKLQFTNEPKAXSTLMAEYQYKFTENSREDLSQFVLSNILANRVSVRCVDEMPLDDLYVLSTSGPFCDCFVRYRDEEKXIYHLYPAHRAVLIRSDYLRAMFSSNFLESSKNSSTDEFNGKVFPIIEFPGTKAEVVETILKYLYYDDIEISVDDAIDILLAADALLLDRLKTIAAITITTNETNFLKKYSIFQILDVAWETQVERLEHFVAKMIAYNLNXYANDESLYRAIKQSATRIQERQATDTIELVDDINYYLLEKYHLLYTDENGDYDYDTLSEEGGEXSMYSKEKDIINRILLKLGLSAFVPETQIVADK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7C5</accession>
    <name>A0A1E5R7C5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uroporphyrinogen-III synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7C5"/>
    <dbReference type="FunCoup" id="A0A1E5R7C5">
        <property type="interactions" value="119"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7C5"/>
    <dbReference type="InParanoid" id="A0A1E5R7C5"/>
    <dbReference type="OrthoDB" id="5595751at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00320"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004852">
        <property type="term" value="F:uroporphyrinogen-III synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006780">
        <property type="term" value="P:uroporphyrinogen III biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06578">
        <property type="entry name" value="HemD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10090">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036108">
        <property type="entry name" value="4pyrrol_syn_uPrphyn_synt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003754">
        <property type="entry name" value="4pyrrol_synth_uPrphyn_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039793">
        <property type="entry name" value="UROS/Hem4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12390">
        <property type="entry name" value="UROPORPHYRINOGEN III SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12390:SF0">
        <property type="entry name" value="UROPORPHYRINOGEN-III SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02602">
        <property type="entry name" value="HEM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69618">
        <property type="entry name" value="HemD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrapyrrole biosynthesis uroporphyrinogen III synthase" evidence="1">
        <location>
            <begin position="18"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="260" mass="28986" checksum="D508D6D50EBBC3FA" modified="2017-01-18" version="1">MSQKIILLKNKTTPFDPYDDLLSKTGFQPTFIPLLKHTHLPDQCLEILNSKQLLELDFLIITSQRAVECVQQSILNCLSSDDKKLLLSKPVYTVGPATAKFITEVGFEDVRGGLEAGNGSILSDLIINDLKDNSNTSNILFLVGEVRRDIIPKKLSPFTNIKLQEVVIYKTECLDDITDRFNEHYLPSTKNVVVFFSPQGTEYIIPQLGNLSASETPKIISIGPTTEKYLTSNSIQVDQVCAKPDARSLLACIQRVLESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7C7</accession>
    <name>A0A1E5R7C7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein mS35</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">37S ribosomal protein S24, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3138</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the mitochondrion-specific ribosomal protein mS35 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7C7"/>
    <dbReference type="FunCoup" id="A0A1E5R7C7">
        <property type="interactions" value="159"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7C7"/>
    <dbReference type="InParanoid" id="A0A1E5R7C7"/>
    <dbReference type="OrthoDB" id="283424at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017081">
        <property type="entry name" value="Ribosomal_mS35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019349">
        <property type="entry name" value="Ribosomal_mS35_mit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039848">
        <property type="entry name" value="Ribosomal_mS35_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13490">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13490:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10213">
        <property type="entry name" value="MRP-S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036995">
        <property type="entry name" value="RSM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein mS35 mitochondrial conserved" evidence="3">
        <location>
            <begin position="140"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="261"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR036995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ82812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="292" mass="33886" checksum="F07947E6BC643964" modified="2017-01-18" version="1">MFRLATRQKRFLHSTSGLFNAAGTPTKADVFNPKTWKGLPAPQVLDLYYARHQELGRNYKPNNDELSALMGIASDIGMDAKDLKTLYDANEFFYIPHTGKNEVTELPSLAQDLVQEHREQRFYNRLAAHDLPQLVQFRKPYQRPDLKTHPITYKYTQYVGEEHPNSKKVVLKCNVADLPNMSEQELHALKLLATTRYDYQTDVLKFSSDRFPEPRQNAKYLSDVLNKLILEAKANAKEFKDVPLDKRHINARTMRKKTKGKINEFPKEWERPQDAPKEPFDAARLILKSKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7H3</accession>
    <name>A0A1E5R7H3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">RuvB-like helicase</fullName>
            <ecNumber evidence="15">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g3153</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA helicase participates in several chromatin remodeling complexes, including the SWR1 and the INO80 complexes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the RuvB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="15"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7H3"/>
    <dbReference type="FunCoup" id="A0A1E5R7H3">
        <property type="interactions" value="1596"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7H3"/>
    <dbReference type="InParanoid" id="A0A1E5R7H3"/>
    <dbReference type="OrthoDB" id="10060499at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097255">
        <property type="term" value="C:R2TP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000010">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.360:FF:000001">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.360">
        <property type="entry name" value="RuvB-like helicase, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027238">
        <property type="entry name" value="RuvB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041048">
        <property type="entry name" value="RuvB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042487">
        <property type="entry name" value="RuvBL1/2_DNA/RNA_bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010339">
        <property type="entry name" value="TIP49_P-loop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11093">
        <property type="entry name" value="RUVB-RELATED REPTIN AND PONTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06068">
        <property type="entry name" value="TIP49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17856">
        <property type="entry name" value="TIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 15" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 15 17" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 15" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="67"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ82831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="461" mass="50176" checksum="84FC5BAD874055FB" modified="2017-01-18" version="1">MVEVKNSESGSSDSATYTRTAAHTHIKGLGLDDAGVAKPIEGGFVGQIEAREACGVIVDLIKHKKMSGKAIMLAGGPSTGKTALALAISQELGPNVPFCPLVGSELYSVEVKKTEALMENLRRAIGLRIKEVKEVYEGEVTDLTPEEAENPLGGYGKTISHVIVGLKSAKGTKTLRLDPTIYDSIQKEKVTIGDVIYIEANTGAVKRVGRSDAYATEFDLEAEEYVPLPKGEVHKKKEIVQDVTLHDLDIANARPQGGQDVISMMGQLMKPKKTEITEKLRQEVNKVVSKYIDQGVAELVPGVLFIDEVNMLDIEIFTYLNRALESEIAPIVVLASNRGMTTVRGTTDIVSPHGVPSDLIDRLLIVRTVPYNKSEIREIISKRAVVENLKLQDAALDLLAELGSNTSLRYSVQLLSPSGILAQTYGRLEIAVSDIEDAKSLFLDSKRSTKILEESASKYLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7L0</accession>
    <name>A0A1E5R7L0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">mitochondrial processing peptidase</fullName>
            <ecNumber evidence="5">3.4.24.64</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Beta-MPP</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g3140</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalytic subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins. Preferentially, cleaves after an arginine at position P2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.</text>
            <dbReference type="EC" id="3.4.24.64"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the peptidase M16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.64" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7L0">
        <property type="interactions" value="884"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7L0"/>
    <dbReference type="InParanoid" id="A0A1E5R7L0"/>
    <dbReference type="OrthoDB" id="10251424at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000002">
        <property type="entry name" value="Mitochondrial-processing peptidase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF149">
        <property type="entry name" value="GH01077P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="15">
        <location>
            <begin position="51"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="16">
        <location>
            <begin position="203"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ82899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="480" mass="53030" checksum="03320311AF0ED63A" modified="2017-01-18" version="1">MLRTLQQASTRLNNRTPXFLNKLSRAXSCNARPVSTVSFTQTSVLPNGITVASEHLPNTSSATVGVFVDAGSRAENVVNNGTAHFLEHLTFKGTARRSQEKIELDIENIGSHLNAYTSRENTVYYAKTLKEDISTSMDILSDILTKSVLDPKAIERERDVIIRESEEVDKMYDEVVFDHLHEIVFKDQPLGRTILGPIPNIKKITRKDLSDYIQTNYKGDRMVLVGTGAINHEDLVKYGEKYFGHLPKSSKVVPLGSPRGPLPVFNSAEKKLQDSTIPTTHIAIAMEGCSWSSPDYFTGLCTQAIIGNWDKAMGSGTNSPSPLAVAASTKQGTDGLANSYMSYSTSYCDTGLWGSYIVLDSQLHDPKKIIDEILNEWKRIKLGHINKDEFERAKAQLKASLLLSLDGSTPILEDIGRQIVTTGKRLTPEEVFNQVDKIGIADIVKWANATLNENKPISICSMGNTEKVPSLEHLKEGLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7L5</accession>
    <name>A0A1E5R7L5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Antagonist of mitotic exit network protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3262</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7L5"/>
    <dbReference type="STRING" id="56408.A0A1E5R7L5"/>
    <dbReference type="InParanoid" id="A0A1E5R7L5"/>
    <dbReference type="OrthoDB" id="550575at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF95">
        <property type="entry name" value="F-BOX PROTEIN YLR352W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="498" mass="56510" checksum="8608D24F7728484B" modified="2017-01-18" version="1">MKRILFQTDVNIFQSKKHQSPQHIIQDKLNNNDDTGFSNKNNSNSSTTLVNDLLPELNVKKLNCPTTLDTPVKPKQFKELAPRKASLCKSSSEATLLVSSLGFHVSPKVRITSSSSLLASSSPFFSPQQTFSSPDTDSIPLIPCHAIFEIPEIVSNIVFFLHLNSPHLRETVSVNKTFHHISSQILNKDLKITNSGQLQKFNNHYEFKTPSSRKSPERLTIVCDDSSNLQTLNVNFHNLKYLEIKNCKKMISLPRSLVNSFILNSTTLHTLKLPGCINFSNTGLKEILGALQKAQEIDLRQCYRLTDNSIMWLVSQCSQLTSLNLNRKPPLSCTLDNYYSKYALSDRVLHAIIDNNLPLHTLGIAGSNISDHALWELCTGRATHFNYKDKMHKVRYTLKRLSCNDCRELTELSLPCILKNNGFPNLQVLEIKNNTKLENHMTDIFKFKISKELNGIPILVSCCDYLQQKMNELQDSYNVNVIEKVLQDMTLWCNSHDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7Z5</accession>
    <name>A0A1E5R7Z5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cytochrome c oxidase assembly protein COX11, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3387</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Exerts its effect at some terminal stage of cytochrome c oxidase synthesis, probably by being involved in the insertion of the copper B into subunit I.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
            <orientation evidence="2">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7Z5"/>
    <dbReference type="FunCoup" id="A0A1E5R7Z5">
        <property type="interactions" value="518"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7Z5"/>
    <dbReference type="InParanoid" id="A0A1E5R7Z5"/>
    <dbReference type="OrthoDB" id="1704689at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.370.10:FF:000001">
        <property type="entry name" value="COX11 cytochrome c oxidase assembly homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.370.10">
        <property type="entry name" value="Ctag/Cox11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00155">
        <property type="entry name" value="CtaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023471">
        <property type="entry name" value="CtaG/Cox11_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007533">
        <property type="entry name" value="Cyt_c_oxidase_assmbl_CtaG"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003465">
        <property type="entry name" value="PRK05089.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21320:SF3">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX11, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21320">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04442">
        <property type="entry name" value="CtaG_Cox11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110111">
        <property type="entry name" value="Ctag/Cox11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="7">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ83027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="310" mass="35333" checksum="423E7A798E4A7C7E" modified="2017-01-18" version="1">MLKTFTLFETSLPLRSPMLCRVRSLYTKSNSPLHLPNFRTTSHTQRNIPLCFAYSARRKFTNVCPLRSETTPPTSEPSKTEKEAEIQRILSTLSSKELAELRRMKHEKENAYKDKSMTYYFSSIILVFLGLAYAAVPLYRAICARTGFGGIPITDKRKFTNDKLVPVASEKRLRIRFSSEVSNILPWTFTPEQKEVFVLPGETALAFYKAKNTSDKDIIGMATYSITPGEAAQYFNKIQCFCFEEQKLNAGEEIDMPVFFFIDPEFANDPAMKNIDDIILHYCFFKAHYSDGSAVSDGVLEVVDAKNHDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R828</accession>
    <name>A0A1E5R828_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein translocation protein SEC63</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3445</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R828">
        <property type="interactions" value="485"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R828"/>
    <dbReference type="InParanoid" id="A0A1E5R828"/>
    <dbReference type="OrthoDB" id="1734229at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031207">
        <property type="term" value="C:Sec62/Sec63 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006620">
        <property type="term" value="P:post-translational protein targeting to endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24075">
        <property type="entry name" value="SEC63 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24075:SF0">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC63 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="131"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="640"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="644"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="670"/>
            <end position="688"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ83062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="688" mass="79239" checksum="D19D8D6B130A627E" modified="2017-01-18" version="1">MVLNYDYDESSETWPFFLITVGSAILIPFTISHFLSLFKKDADYFENIQQTYKDNKELLNLSKEIQDLNNEYTDADMQKLRSKYESKQKRSKIFNWKTYVLAASWMLMAYLIQSTLSNKSIFEKAAKELFDPYEMLGLASSSTDKEIRSAYRKLSVRLHPDKMPKNLSDMEKLKFEEDYVLITKAYKALTDELTKENYLKYGHPDGPQSVSHGIALPKFLVDSSASPYILAIYIGILAFILPYFVNKWWTSSKLYTKTGIHVESASFFISKVFNYKPSEIVTVDTILHWLSHAEEYKILYPEHSPETFYQLLKNHIERKPNGSKQLDDISFRVVAKTHSLLYSLLNISTVFRNVDITLKLLETFKSIVQAVDSSRSKHELLQLPNVGELPDTKTLENPQLGSVKTLGKLFTLSEDDKAKVLGLDKKSKEFKDAMKVASDIPQLLLLRAKFVVPGEKVVTPGSLPHIDLKVLVRSPKSKFISGEIFPEDKINYYHDDFENLKDPFQKIAEQPIVPQTFAPRFPVLRHSTYATLVINQTDNKIIQTPSKTNNLHFENIKNEAILSQFDKYLTKDVKLDKENFKADEWKVSTIKIPFGQPAPQEKGITFYRIVVSNTDFFGGDLDFTMPMEVKDLPPLTEAEKLEIEMQEVYESESESDDDDDDEKDEHXSDYDSESDFTDIDTDTEDEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R872</accession>
    <name>A0A1E5R872_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Metacaspase-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3354</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C14B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R872"/>
    <dbReference type="FunCoup" id="A0A1E5R872">
        <property type="interactions" value="374"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R872"/>
    <dbReference type="InParanoid" id="A0A1E5R872"/>
    <dbReference type="OrthoDB" id="3223806at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12660:FF:000005">
        <property type="entry name" value="Mca1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050452">
        <property type="entry name" value="Metacaspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48104:SF30">
        <property type="entry name" value="METACASPASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48104">
        <property type="entry name" value="METACASPASE-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase C14 caspase" evidence="9">
        <location>
            <begin position="161"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="115"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="72"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="87"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="115"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ83106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="460" mass="51275" checksum="56DCACAAB855D893" modified="2017-01-18" version="1">MFPGSGRYTYNNNQQQQQQQQQQYSRPSAPPPGQYQGEQQYSRPSAPPPGQYQQEQQQYSRPAAPPPGSGSNRYPDNGLNSSDYSFTNVNGNTNGNINPQAMQQQATMNNNASYSNAQANQQQSSANSHYVQPQQQYRPPSQVQTSANTNTQFQYSQCTGRRKALIVGINYFNSQNELRGCINDAQNMFNFLCSQYGYSHDDIVILTDDQSEMGRVPLRANIIRAMQWLVKDARPNDSLVFHYSGHGGQTKDLDGDEDDGMDDVIYPVDFQQTSHITDDEMHDIMVKVLPQGCRLTALFDSCHSGSVLDLPYTYSTKGVIKEPNMYKALGQEGLQTVLSYATGNKQAMMSGISSIFKTVTSGGSHLSAQQREQVKQMKFSPADVVMFSGCKDDQTSADASENGRATGALSWAFMKVIGQQPQQSYLTLLQNMRYELSSKYSQKPQLSSSHPIDVNLQFIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R880</accession>
    <name>A0A1E5R880_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Aspartate protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g3422</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R880"/>
    <dbReference type="FunCoup" id="A0A1E5R880">
        <property type="interactions" value="424"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R880"/>
    <dbReference type="InParanoid" id="A0A1E5R880"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000036">
        <property type="entry name" value="Vacuolar aspartic protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000002">
        <property type="entry name" value="Vacuolar aspartic proteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF51">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="8 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 13" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184643" description="Aspartate protease" evidence="14">
        <location>
            <begin position="20"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="15">
        <location>
            <begin position="107"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="310"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="138"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="344"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ83110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="421" mass="46368" checksum="E82DA3BC7DC9060F" modified="2017-01-18" version="1">MQFQIFLTLAALFIAECQAKVHSTNLKKTKLEDQLKDLTFDQYVASLGAKYTHMYQRAFPDEEIQFESTNLKDRIMNSFSNHFTNQDSSFRELSTSLPLSNYANAQYYTEISLGTPAQNFKVILDTGSSNLWVPSAECSSLSCFLHAKYSHEDSSSYKKNGTEFAIQYGSGAVEGYISQDTLVLGDLLIPEQDFGEVTSEPGLTFAFGKFDGILGLAYDSISQGKVVPPIYNAIAQDLLDEPKFAFYLGNADVDPEDGGVATFGGIEKSRFTGDITWLPVRRKAYWEVKFDGIGLGKEYAELEKHGAAIDTGTSLITLPSQLAELLNSEIGATKSWTGQYTVECETRDSLPDLTLTFAGKNFTLSPYEYTLELSGQCISVFTPMDFPAPIGPLAIVGDAFLRKYYSIYDLGNDAVGLALAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R8A1</accession>
    <name>A0A1E5R8A1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein MGR2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3326</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MGR2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R8A1"/>
    <dbReference type="FunCoup" id="A0A1E5R8A1">
        <property type="interactions" value="248"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R8A1"/>
    <dbReference type="InParanoid" id="A0A1E5R8A1"/>
    <dbReference type="OrthoDB" id="5409308at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018450">
        <property type="entry name" value="Romo1/Mgr2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28525">
        <property type="entry name" value="REACTIVE OXYGEN SPECIES MODULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28525:SF1">
        <property type="entry name" value="REACTIVE OXYGEN SPECIES MODULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10247">
        <property type="entry name" value="Romo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01378">
        <property type="entry name" value="Romo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ83130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="118" mass="13041" checksum="3ED67407A8BD7191" modified="2017-01-18" version="1">MPPINPAIYSQQPSNFDKFKMGLMMGTTVGVVTGIFFGGFAILTQGTGSQGAVRTLGKYIFGSATTFGLFMSVGSIIRSDTEELNKHRNTEHNYSRARLQYDAMRLRAQHGLFTPRND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R8A2</accession>
    <name>A0A1E5R8A2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">tRNA (guanine-N(7)-)-methyltransferase</fullName>
            <ecNumber evidence="11">2.1.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Transfer RNA methyltransferase 8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA (guanine(46)-N(7))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA(m7G46)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TRM8</name>
        <name evidence="13" type="ORF">AWRI3579_g3202</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42708"/>
            <dbReference type="Rhea" id="RHEA-COMP:10188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10189"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74480"/>
            <dbReference type="EC" id="2.1.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10 11">tRNA modification; N(7)-methylguanine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Forms a complex with TRM82.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.33" evidence="11"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R8A2"/>
    <dbReference type="FunCoup" id="A0A1E5R8A2">
        <property type="interactions" value="545"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R8A2"/>
    <dbReference type="InParanoid" id="A0A1E5R8A2"/>
    <dbReference type="OrthoDB" id="47276at2759"/>
    <dbReference type="UniPathway" id="UPA00989"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106143">
        <property type="term" value="C:tRNA (m7G46) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008176">
        <property type="term" value="F:tRNA (guanine(46)-N7)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000060">
        <property type="entry name" value="tRNA (guanine-N(7)-)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03055">
        <property type="entry name" value="tRNA_methyltr_TrmB_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025763">
        <property type="entry name" value="Trm8_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003358">
        <property type="entry name" value="tRNA_(Gua-N-7)_MeTrfase_Trmb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00091">
        <property type="entry name" value="tRNA (guanosine(46)-N7)-methyltransferase TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417">
        <property type="entry name" value="3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417:SF16">
        <property type="entry name" value="TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02390">
        <property type="entry name" value="Methyltransf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51625">
        <property type="entry name" value="SAM_MT_TRMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 11 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 11" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3 11" id="KW-0820">tRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="119"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="154"/>
            <end position="155"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="252"/>
            <end position="254"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ83139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="280" mass="32716" checksum="0D32025851809509" modified="2017-01-18" version="1">MGSNCNATQRKQYRDERDSKRSALKHVQFPENEKTGQFDLPKKKFYRQRAHSNPFSDHKLEYPSCPNDMDWKKLYPGFIDPETNELTSKVTIADIGCGFGGLLIDLSPEFPQNLILGMEIRVQVTNYVEDRIIALRSQNKDTKAYQNISVLRGNAMKFLPNFFEKSQLEKIFFCFPDPHFKQRKHKARIVTNTLLSEYAYVLKEGGIVYTITDVLDLHEWMVKHLEEHPLFERLDKEWESQDKCVQIMTNATEEGQKVERKKGAKYIACYRRVSNPVIIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAG1</accession>
    <name>A0A1E5RAG1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Double-strand break repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">AWRI3579_g2783</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Core component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage. Within the MRN complex, MRE11 possesses both single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity. MRE11 first endonucleolytically cleaves the 5' strand at DNA DSB ends to prevent non-homologous end joining (NHEJ) and licence HR. It then generates a single-stranded DNA gap via 3' to 5' exonucleolytic degradation, which is required for single-strand invasion and recombination.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16 18">Belongs to the MRE11/RAD32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAG1">
        <property type="interactions" value="1032"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAG1"/>
    <dbReference type="InParanoid" id="A0A1E5RAG1"/>
    <dbReference type="OrthoDB" id="3971887at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030870">
        <property type="term" value="C:Mre11 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008296">
        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042138">
        <property type="term" value="P:meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097552">
        <property type="term" value="P:mitochondrial double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.110">
        <property type="entry name" value="Mre11, capping domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003701">
        <property type="entry name" value="Mre11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038487">
        <property type="entry name" value="Mre11_capping_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007281">
        <property type="entry name" value="Mre11_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00583">
        <property type="entry name" value="mre11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10139">
        <property type="entry name" value="DOUBLE-STRAND BREAK REPAIR PROTEIN MRE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10139:SF1">
        <property type="entry name" value="DOUBLE-STRAND BREAK REPAIR PROTEIN MRE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04152">
        <property type="entry name" value="Mre11_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000882">
        <property type="entry name" value="DSB_repair_MRE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01347">
        <property type="entry name" value="Mre11_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 16 18" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 16 18" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 16 18" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11 16 18" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="10 16 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 16 18" id="KW-0464">Manganese</keyword>
    <keyword evidence="15 16 18" id="KW-0469">Meiosis</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 16 18" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14 16 18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mre11 DNA-binding" evidence="20">
        <location>
            <begin position="322"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="600"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="656"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="731"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="607"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="752"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="776"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="17">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="21">
        <location>
            <position position="266"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="21">
        <location>
            <position position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000882-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM01347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OEJ83895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="807" mass="91058" checksum="18A8031DD1BC2DCD" modified="2017-01-18" version="1">MDGQQTLSSKNGSIEGPGSLTEYPGENVIRILVSTDNHIGYSETDPIMGDDSWKTFFEIMELGTTYNVDFFLQSGDLFHVTSPSKKSFVRVSEALSKYCWGDKPVELEXLSNPGLSINNSNPHLNDVNWRXPNLNISFPFFAISGNHDDSTGRDQFSAMDVLHSTGLINHFGKTMTVTDIDIHPILLRKGTTNLALYGLNAIRDERLYKSFKLGKVNFNYPKLPATHNDLEKSNKQFFSLMCVHQNHTGHTNTGFLPEICLPSFLDMVIWGHEHECIPSIKHNTERDFDVLQPGSSVATSLSEAEGKPKHAFILEIRNNQEYSVIPLPLNTVRPFIMEDISLSNFKELESHNKVQIQKFLLKTVDQLVKRANQESERRIIETTDLTDREEILKQIPLPLVRLRVDYSCPEDPDSSYMVENSRRFSNRYVGKVANPNNILQLYKKKHKSYVKDYEKDKLSKLNKKMGAFDMDEYDEDGGKNFANADEEILAIIESTLKSTNLALLSEXGISEALRKTVLNDDKTAMKSFLENQVSKNTKLILDNNLLIESHNIKDFKDILKKVAQPSKIHGSPKDETEFAISNPATSDVFLETARKELLQSGALPTAEKSRVPERRRATDTKIKTLRTSXSKTIISDDENFDLNNENDDEDFILSSHXEESDEQVGKKDTRRVSGKQRRIVDNSESQNXXADSIENGLESVQVSSNEDEGFSSPGHSYVAATLDEPMIIDSDNEDFEYGTPFQAAPAKRKPTSAESSKSGKPTTKKRTVSXNTSKXKHAESKQEYKPVTKLSQSSKLMDMLNSRKTRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAL7</accession>
    <name>A0A1E5RAL7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Protein SOV1, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2495</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAL7">
        <property type="interactions" value="277"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RAL7"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ83937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="892" mass="102797" checksum="16E2A9A4E6C8BDD0" modified="2017-01-18" version="1">MLKSFFTLQGKTPIFLLPTRTFRLQQKLCFHVSTLLKARSNSRAIVKKLELIKKERELGNTETSKYIEKELDTQGLNLIDPTADLSQTFIEYMKLNHTLFNKTLKHLKRLEKELTTQQYTEDEKFHKLFEFFSVECAKEVERLSKMGIENLDNMLSEKHLQDLQKLNETLETDPNENILMEKFTKDFFQSAESSSLTDTQSNGGLPVLKMTTTLFSLLEVLLKTDSSSSIHKIMSPMDYVQIFEISKNIPINFLKMKGFYLSGTLIYNIKNSQGKSLTMDPINESFFIDSLMAQGQYKKALILFKNKLLKVNQKWWMEMGMMLYLKTNQLNKFNKLFKYTVAKFGEEQINVKPVKLAILGNLKVKNTEMFYRYLNFYKNKVSQYGLIPNEVYLSNINSTNVQFASEEEGNVFLNKFDGIRDKNFFSVVSILIYNNHLKEAQELLTMYENDKPGFDPHNPRYQINRLHAVLKSIVTQSIGCPYSKATIPQMLQELQVSCELLQDSPNSIPVLQNLMIKSVMALAKNPVYSDDIASLFTSDLQAQILGNEQMSDGEKYKTIVMFLLKSNCIAPALNILSILEKSGAVTTHIYAEFIRHFTFQITNDKKPHSKTHAEACKTVDAFLEKIEKDTSIVVSNSFIDSLIQFFTATNQWDKLVPFVNKYLESLISNNQGTNCKDQLSLSPSVMNKVFHVYGTYYHKTASLPLFKDFNKLDTKSNFTYHIDSLLRFCFFEKNMVPNAQIYQTIVKSLVLSQNWTSLIAVILYCVENYDLRLAEPTFKYILKGIKFSYLGIEMKTGSESGSSWKEMKLQKSKALKKINQAIHSKEIMKFVTMENSSNETVTICSILEYLMDIHEYKQSIIEEKLVNFGLSKNLIXDDLNLVQKSSSNQDLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAM8</accession>
    <name>A0A1E5RAM8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nucleolar protein 56</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2959</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for 60S ribosomal subunit synthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP5/NOP56 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAM8"/>
    <dbReference type="FunCoup" id="A0A1E5RAM8">
        <property type="interactions" value="1466"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAM8"/>
    <dbReference type="InParanoid" id="A0A1E5RAM8"/>
    <dbReference type="OrthoDB" id="6780543at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.90:FF:000001">
        <property type="entry name" value="Nucleolar protein 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.4070:FF:000002">
        <property type="entry name" value="Nucleolar protein 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045056">
        <property type="entry name" value="Nop56/Nop58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012974">
        <property type="entry name" value="NOP58/56_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894">
        <property type="entry name" value="NUCLEOLAR PROTEIN 5 NUCLEOLAR PROTEIN NOP5 NOP58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08156">
        <property type="entry name" value="NOP5NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Nop" evidence="9">
        <location>
            <begin position="297"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="432"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="433"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="467"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="487"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="497"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ83958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="512" mass="57727" checksum="CAFBDE81C6564FB1" modified="2017-01-18" version="1">MSAELEYLLFEEPTGYAIFKVKLQEDTVNSSSKEVQKQINDFGSFTKLVELVSFAPFKGAAQALENANEISEGLVSESLQSILELNLPKTKTVLAISDSKLGPSIKDVFPNVSCVANDLAQDLIRGVRLHSTKLLKSFVEKGDLEKAQLGLGHAYSRAKVKFNVSKNDNHIIQAIALLDSLDKDINTFAMRVKEWYGWHFPELAKIVPDNYTFAKLVLFIRDKASLTEDSLHDLVEVLNNNEAGVAEKVIDNARISMGQDLSDIDMDNVCVFAQRVVSLVEYRKQLFDYLCDKMHTVAPNLSELIGEVIGARLISHAGSLTNLSKQAASTVQILGAEKALFRALKTKGNTPKYGLIYHSGFIAKASAKNKGRISRYLANKCSMASRIDNYSDEPTNIFGTVLRKQVEQRLEFYSSGAPTLKNELAIQEAMELYNKDKPEEPKEKSKKRKLEADAEEEEEPKEEPKPKKEKKEKKEKKEKKEKKEKKEKKDKSEKKEKKDKKEKKDKKKSKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAU0</accession>
    <name>A0A1E5RAU0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">D-serine dehydratase</fullName>
            <ecNumber evidence="11">4.3.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">D-serine deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g2815</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the conversion of D-serine to pyruvate and ammonia. May play a role in D-serine detoxification.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-serine = pyruvate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13977"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:35247"/>
            <dbReference type="EC" id="4.3.1.18"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:13978"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DSD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.18" evidence="11"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAU0">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAU0"/>
    <dbReference type="InParanoid" id="A0A1E5RAU0"/>
    <dbReference type="OrthoDB" id="20198at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008721">
        <property type="term" value="F:D-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036088">
        <property type="term" value="P:D-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.10:FF:000016">
        <property type="entry name" value="D-serine dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.20">
        <property type="entry name" value="D-serine dehydratase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051466">
        <property type="entry name" value="D-amino_acid_metab_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026956">
        <property type="entry name" value="D-ser_dehydrat-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042208">
        <property type="entry name" value="D-ser_dehydrat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28004:SF2">
        <property type="entry name" value="D-SERINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28004">
        <property type="entry name" value="ZGC:162816-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14031">
        <property type="entry name" value="D-ser_dehydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01119">
        <property type="entry name" value="D-ser_dehydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0216">Detoxification</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="D-serine dehydratase-like" evidence="14">
        <location>
            <begin position="300"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00066349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00075219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ84019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="420" mass="47401" checksum="A5D567712F11765F" modified="2017-01-18" version="1">MSNVTKNKVLQQYKGCFIEELPSPSFLIDEQKFDANCLRMVESIKRLQELALCSMSFRPHVKTHKTVEGTIKQLNNGFSKSVLVSTLSEAEGLINDQRSSSQIDDICYTLPLSFDMAFMQRIAAIKEKKFLRIFIDNVAQLPYLKDYQTLLXDAEPWSVFVKIDVGTARAGIEAESAEFMDLISAIHGQYSSVVSLHGFYAHCGHSYHVENVEEVYTDLVEELRGVVRSAQIFSEYSNKKLVLSIGSTPTMNALNDRPEIRELLRQIGHMGHTLEFHCGNYCLLDLQQVSTGMVKLEDIAGFVLGTVVSKYEKRKEFLMNTGVLSLTREVSDGFPGFGVVFPMSFDKNHYXDVSKTFIVERLSQEHGIVKSNDGALLEIATKAAVIPQHACITMNSFPYFFVFDPHTRKVVDVWVPLKGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB05</accession>
    <name>A0A1E5RB05_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="18">General negative regulator of transcription subunit 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g2734</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RB05"/>
    <dbReference type="FunCoup" id="A0A1E5RB05">
        <property type="interactions" value="409"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB05"/>
    <dbReference type="InParanoid" id="A0A1E5RB05"/>
    <dbReference type="OrthoDB" id="1923159at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051254">
        <property type="term" value="P:positive regulation of RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16618">
        <property type="entry name" value="mRING-HC-C4C4_CNOT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12438">
        <property type="entry name" value="RRM_CNOT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000257">
        <property type="entry name" value="CCR4-NOT core complex subunit Not4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000006">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034261">
        <property type="entry name" value="CNOT4_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039780">
        <property type="entry name" value="Mot2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039515">
        <property type="entry name" value="NOT4_mRING-HC-C4C4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12603">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12603:SF0">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14570">
        <property type="entry name" value="zf-RING_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="6 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="15">
        <location>
            <begin position="18"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="122"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="17">
        <location>
            <begin position="203"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="12">
        <location>
            <begin position="203"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="245"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="305"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="349"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="409"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="449"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="504"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="703"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="76"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="306"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="375"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="415"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="519"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="709"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ84074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="747" mass="81559" checksum="E588129CF00B9E01" modified="2017-01-18" version="1">MATAYIEPFLSEDEDEYCPLCVEPMDLSDKHFKPCPCGYQICSFCYNNIRQNPELNGKCPACRRKYDDESVQYVVLSPEELKLEQLKQAKKEKERKIRDKERKENENANRRQLANMRVIQKNLVYVVGLNPSCPYEEVAGLLKSDKYFGQYGRITKIVVNRKENGSNQGYGVYVTFSKKEEAYRCISQVDGTYWDGRIIKAAYGTTKYCSSYLRGQSCPNPNCMFLHEPGEEAEYYNRHGVQNGRPGYNSTTHHSALPKTVPPLKLSAPSNAADTEHVLNSDSSHVYSSASAMSPNLASHYAHTVAQPSQTGSGSTGAASHTTYTNASPSANHIHLNTSNITSASASQLTPAGIPVSNPWGSVKPSQQPTVNPWGARASGTNANSAGGSSAFSSTVGIGSAGVFPSLNQASNAQTPSTSSSLSSSTIDATNKNNLNTLNNLNSPHLSGSSFNALNQSQDHLSSNGKSAFGSNTPSSISSIIGTQNSFNTMPAQHTSIMKSVLKKNGGSSTAMASNSSNKNDKDKEKENNNNQTNIYDPCANAVKLLDDAIDYLSTFKSSSISLRTSIFRNENEQLRYQSMPPLFSYENITPVEKSEGVLRQKLIKMLSVEPTEPDANMSPSASIVHSTNAIEIPSVNASPENPMVSPMANTMFQSPHMFPDQQLAQQQLLQQQQLAQQQLAQQQQALQQQLLLQQQQQQHFAHPPGMFNQKSVQSTPQMAAQSMNLNPSGSSNSTDLLNQLIKGKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB18</accession>
    <name>A0A1E5RB18_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosome biogenesis protein NSA2 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2730</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the biogenesis of the 60S ribosomal subunit. May play a part in the quality control of pre-60S particles.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the pre-66S ribosomal particle. Interacts with NOP7 and RRP1. Interacts with RSA4 (via WD repeats).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
            <location evidence="1 7">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the eukaryotic ribosomal protein eS8 family. Ribosome biogenesis protein NSA2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RB18"/>
    <dbReference type="FunCoup" id="A0A1E5RB18">
        <property type="interactions" value="1254"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB18"/>
    <dbReference type="InParanoid" id="A0A1E5RB18"/>
    <dbReference type="OrthoDB" id="1847590at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11381">
        <property type="entry name" value="NSA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.310:FF:000001">
        <property type="entry name" value="NSA2, ribosome biogenesis homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039411">
        <property type="entry name" value="NSA2_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022309">
        <property type="entry name" value="Ribosomal_Se8/biogenesis_NSA2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12642">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN NSA2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01201">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 7" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="61"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="7"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ84071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="263" mass="30056" checksum="1B8A61657E5935DC" modified="2017-01-18" version="1">MPQNEYIEQHIKQHGRRLDYEERKRKKEAREGHRVAKDAQRLTGWRGKQFAKKRYSEKVAMKKKIRAHEQSKIKDPNNKSPLNEDGDALPTYLLDRETQNTAKAISNSIKQKRLEKADKFSVPLPKVRGISEEEMFKVIKTGKSKSKSWKRMITKHTFVGEGFTRRPVKMERIIRPTALRQKKANVTHPELGVTVFLPILAVKKNPQSPMYTQLGVLTKGTIIEVNVSDLGMVTSGGKVVWGKYAQVTNEPDRDGCVNAVLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB69</accession>
    <name>A0A1E5RB69_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein yippee-like MOH1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3077</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB69"/>
    <dbReference type="InParanoid" id="A0A1E5RB69"/>
    <dbReference type="OrthoDB" id="6407410at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034751">
        <property type="entry name" value="Yippee"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039058">
        <property type="entry name" value="Yippee_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13848">
        <property type="entry name" value="PROTEIN YIPPEE-LIKE CG15309-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51792">
        <property type="entry name" value="YIPPEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Yippee" evidence="2">
        <location>
            <begin position="255"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="349" mass="40388" checksum="32136A8E85D16915" modified="2017-01-18" version="1">MKAAEYDENNFELGRAKFIKSAVNNKSSYTNSFYCYVNEKSDNVETQKEIIKMKNQWGFLCATNEAFHLELNKEIGGKSKVFXQESNNADFQGNPIQYYKANNQHRAQXLETQRKRLYELGKWASLQHHEIIEKLRVKEKIAMGREDEDDVSEESDENGSESEENSDGVDSQLFDDESLSLSQQEDDDFMEDDDSLAEDLLDDDDDDDDDDDDDDDDDDDDEDDLVQSYTHTHPHEHLTLGQSLTKKSKNHGKGVFYKCNKCCTVLQYKHNLISKNYRGKTGSAMLVYDLNNVKYGECVTVKMMTGEYDCCNIYCLCCSHYLGWKYLKASCFDMKYKEGLYIIENNQIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB82</accession>
    <name>A0A1E5RB82_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Aminotriazole resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2651</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RB82"/>
    <dbReference type="FunCoup" id="A0A1E5RB82">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RB82"/>
    <dbReference type="OrthoDB" id="2130629at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF14">
        <property type="entry name" value="AMINOTRIAZOLE RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="234"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="299"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="339"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="379"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="404"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="432"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="467"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="517"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="73"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ84154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="563" mass="61516" checksum="D7D12FCE9AC0DBA8" modified="2017-01-18" version="1">MNSRLDSTNSNDSVHFKDEQLNNELSNNNKENSNIDSSTSSGIISQQNLVAASEQPVSRLGPRPVCFTNKTSELMFVATAVLSQILNISGTSQTLTLFRILSTDLNTSANMESWLMASFSLVTGSLILISGQLGDIYGLKKVLICGYIFAFIWSLILGFTKYTHQATFFIVSRAFQGLGVSCILPNVMGIVGTIYEAGSHRKNLVFALIGAGAPVGAVFGVWWASLIVHFDKHAWSWCFWAYAILLFLTLVLNILFCPNSIPKNSKNIKMDWIGALLAVVGLVLFNFSWNQGAIVGFQTAYVIALLVISSVAIVLFFIYEKNYALYPLVPPSVFMNPNVVSILATLVFGWGSFGCFLFYLFQTRLYLQNMDPLLAGTEFIPFAVFGIIAALTVALTIKKLTAQVLLVFSSIAFCAGSVMLAILPLHQIYWKLSFFTTIILSFGMDLSFPAASIVLSDNLPKANQGMAGSLISTMMNYAMSLFLGVAGVVQTQVSKNIENKHPEYSEQKKTQMSYRGAMYFAVGSAGLAIVISSSYAIATLVFHKGGKNKNTAVYESGDSTRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBA0</accession>
    <name>A0A1E5RBA0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">DNA damage-binding protein CMR1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2941</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-binding protein that binds to both single- and double-stranded DNA. Binds preferentially to UV-damaged DNA. May be involved in DNA-metabolic processes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the WD repeat DDB2/WDR76 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBA0"/>
    <dbReference type="FunCoup" id="A0A1E5RBA0">
        <property type="interactions" value="781"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBA0"/>
    <dbReference type="InParanoid" id="A0A1E5RBA0"/>
    <dbReference type="OrthoDB" id="9890280at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000001">
        <property type="term" value="P:regulation of DNA damage checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050853">
        <property type="entry name" value="WD_repeat_DNA-damage-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14773">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14773:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="362"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="39"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="437"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="438"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ84175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="545" mass="61229" checksum="CEA1DB49C589E97C" modified="2017-01-18" version="1">MAAEELSEFQKKRLENIKRNNALLSKLNLKNISNKISSAAGASANSTAKLKKRAPVTKKTNRVKKETIPTAPLRQSRRLRGQTADGNVLPNVSDSQLFNGKLKDGVKLEDDKENQEWHEELKDTRVSGDVSLGDLLDDSKLASKYSDLSKLSNGDLFEEWKKYEKGRDSSDGITASEKQLHELREEFDLQLYPHFEPNEIKITDERISAMFYHPMVEQKLIIAGDVSGNLGFWKVPELPKGQTMDEEEIPDITKFKLFQKNVGNICCQPSNMSKIVTSSYDGTLRTIDLNNMKSEELLYNVDVGGISDLQFQDENVAFMTTLEGEFLQYDLRVPVKKVGEVLRLADKKIGSFHINPKNSHEIATGSLDRTLRVWDVRKLTGKPEWSQYEDYNSCEIVRQYDSRLSVSAVNYSTYDDTLVCNGYDNTIRLFDVSWNNASPSSSSSSSSSVKKEEGGGSANTSLEPFKTIMHNCKTGKWTSILKARFKLNRDVFAIANMSRAIDIYTSKGDQIAHLQTATVPAVVAWHPFENWIVGGNSSGKVFLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBB9</accession>
    <name>A0A1E5RBB9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Alpha-1,2-mannosyltransferase MNN2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3047</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBB9"/>
    <dbReference type="InParanoid" id="A0A1E5RBB9"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646:SF1">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="72"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ84195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="787" mass="92333" checksum="8C527726C4CAC20D" modified="2017-01-18" version="1">MRLNLTRLSRSFLQSFKRYAISGGNTAATRRKRVLALGFVFALXIITLVFAXPXLMDFDQLRKNFIKTSSSXMXSTNNNSNNNDDKTSGSENSXVEKQFAHDITSNQQFVSTLFDIIQNFSPDASEHKLSVDRSLDSCKLQDLALWDGNWDLLSEEALKGCIPSPNDAFYEKLSTLHSDFLNNLHELIQKDSQDVDHQGKSNGILENSFTEKQGILLIGGEVYTLFLLGVIRSIRLNSGISKIPIQIIFPXYSKETQYFCEKIIPQIDSENHKDAIHCDYMNDILSHDQLSHLKKYQYKPMALLMSRFQKTLYLDSDAYLAGSIHQWFDDELMEEKGFIMWPDYWRRVHHPKLYEFLDINQDIISEKNRTRVGFINFLPQQIMEKLPLHHDFTSSSLSGSLPFYGKSNDALSEQETRKAKKQMIPFHDYEGTIPDLSTESGILLFDKSKHLETLALALYYNYNGPHLYYTLLNQHLAGEGDKDTFILAATVLKTKYHQVMGPPQAHGYWCDKSEISVADVPEFQKDFHNDLMDLSEKWRFRGTTILQRDFIHDYKLQKWFEKQYLFNLEQDKKQYLKKHHNIDFSMEKEDKDKDIDLESEKDELSKKQDTKQYEHLLSDFYQQKANEFDMQYYIELFKLQTHFPVFHFNLPKFDPYVYAKEEDGMYDGAKFKGREMGRAAKHLXDKKGHFRMLGMEFLQTIGNYDIELSNFKIYKDLFCSYGWNKDKTDEKSINRINYLKEKFMEKPAEETSSENKKEDXITMDKMCVYINDRYDYLMNTTWEDSIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBC9</accession>
    <name>A0A1E5RBC9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Actin-like protein ARP8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2659</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBC9">
        <property type="interactions" value="909"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBC9"/>
    <dbReference type="InParanoid" id="A0A1E5RBC9"/>
    <dbReference type="OrthoDB" id="5572108at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="326"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="682"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="829"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="57"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="327"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="698"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="829"/>
            <end position="855"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="921" mass="103957" checksum="193157678BA065C5" modified="2017-01-18" version="1">MSTAEKIHGKKRNRDQVDDGTAPGQENNFNDVDNDDQIDTPSIAPFSSVTNTPLELSPVPSNSQQPVSSSPVXAPIITNTDTNTDTNTNTSTNTNINTNTNINTNATNSKSNNRGSNSNKKNSSGSNYVGAGKKVPMHLLEKRRLGRIKAAEEFAKKLKTIGIEKIENNNTTSFIKPLNLINLKNISSNYLKNDDQLFASKEKQXNLNDNLPAIVIDPGSKFLKIGFSTDEQPVIVPNCVASLKHXGFSTPMQPLINXPMDISTTTDFIETEKIMRQNFIERMRYYKRKVSPNEYRNQVLNFNSKHANIETIPINQDIHRPKWINGENANENANENANENANENANENANENANENANENEKKEKSVQNKLYFAEEASLCDPSKFDIRYPFANLNVSGLIDPYYTEHHLASGEWVMPLWNDVYCLLMHVLQTKMNLTLKDFGKYNVVLVVPDLYTRPYVEDFIRMILYEFGFAQVAIIKQSVSITYGAGLSQSSCIVDIGSANTKISCLLDGTVVPDSEVVLNYGSQDLNLVLGNFLKKMEFPLKFDIQSRSGNYMLETLKNQYCTFEDSDVTVQLFSNCIKYAESEKEHSMKTDFKIFDEVFLTPLTLFYPKIINXLHQLQKYENFESNLINKSNDIYTENMNNPVSKSQQNLIENKAYCESYEEYDTMKNLINVFLXNSEKQNSGNNDNGDDETSNPKQSLNTNNSLNYYPLDKAIIESIVNATVSCQQYSKLKLFYNNILVVGGGSQIPNLDVLLMDRLNIYRPRMLATTYLPVVLKNLETNLRKLNTNVIENGILNQEKLYTVDDIIEMTLSEYHKKQRDSELFATTPSSVAATNTQHATGKNKGNGTAKNAATEEEPEEVLPVNIIPSPIDPQYISWKGGCVFASIKLIEDLYVTKQDWDMLGSRILTNKCLFEYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBJ0</accession>
    <name>A0A1E5RBJ0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Shugoshin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2514</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the shugoshin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBJ0">
        <property type="interactions" value="165"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBJ0"/>
    <dbReference type="InParanoid" id="A0A1E5RBJ0"/>
    <dbReference type="OrthoDB" id="5394106at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000779">
        <property type="term" value="C:condensed chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045132">
        <property type="term" value="P:meiotic chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011515">
        <property type="entry name" value="Shugoshin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011516">
        <property type="entry name" value="Shugoshin_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07557">
        <property type="entry name" value="Shugoshin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07558">
        <property type="entry name" value="Shugoshin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-0137">Centromere</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Shugoshin N-terminal coiled-coil" evidence="11">
        <location>
            <begin position="75"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Shugoshin C-terminal" evidence="10">
        <location>
            <begin position="557"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="185"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="469"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="185"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="375"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="469"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="494"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="522"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="541"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ84270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="820" mass="92188" checksum="00749257AE7D8D84" modified="2017-01-18" version="1">MLNNMKSGPKGHSKSRRSYTSRKPPGRKTLQRTTNSASQNSLVYERPSYNEINEMQHFQNILDATRTEFEREQHTYTANNSQLAKQLSSVTTKLSDMEKKLAVMMQENVLLRSKNTLDRMNYKKALKNHLEILEKGFSFKIDEILHMFNKVREREGLELKQYGRILLPTNPDFSRCDMNSASNIYSKSTNVTPNHKSILKSFGSTPSVERVSAEQYSSGKRSITFEHGPSLNESPLQGSPKDRSKKNRDRTKEINDNGGKRMEITKDTKITNDMNMEENLAEKEDKEDHRKKRRKSSRRESLFSAEEFGFAQDSHDGPSNNTQPDVSESSKAGQSNGKHRSNTKADLSVIASQHSESEDQIPLDNDDLPHLYVGNKDDAQHTCDLDSEEKASVEIDSEKEXSEKEDSEREDSNDIHEYHTNGTSFSLIDATIPEDSLPQQSQSQPPTVISTIPTKAMVQRSLSPSFFAAEAGSSSSSQESGKSQVYXDEENEGLTKSVSNASSQQSQHSEPSKDVPTIKILHSMKSRPKNKSKTDDIMPSTALNSKMSLSNEVPLSGRSRRTRNKTVNYTQPSLRAKMRRPTEKLVDATTFTNIHELQVSKRSASSKKKKKXANVLKAPVDGFSYCLKFSVGSCVLGKTRLELPKGKELLLKLADDVKDKPFSGSSNTTPQPYLSENRINAAKNKISEAGKKKLPLMDITPRPNSSASNISLPLKSGLQKSFNADISNISNTSMNKTKRLLKKPVMNILDGLDKDYDDDNDENRDPRMKTLRPALANNRMRISEDDLAVFDILDSLDIKKNPKTHKKYSTSKNTKKLLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBL1</accession>
    <name>A0A1E5RBL1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Serine/threonine-protein kinase BCK1/SLK1/SSP31</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3119</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBL1">
        <property type="interactions" value="448"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBL1"/>
    <dbReference type="InParanoid" id="A0A1E5RBL1"/>
    <dbReference type="OrthoDB" id="266718at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000196">
        <property type="term" value="P:cell integrity MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000182">
        <property type="entry name" value="MAP kinase kinase kinase mkh1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000387">
        <property type="entry name" value="Serine/threonine-protein kinase STE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF48">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE BCK1_SLK1_SSP31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="1248"/>
            <end position="1513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="111"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="267"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="619"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="800"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="859"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="953"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1116"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="128"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="306"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="338"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="391"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
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            <begin position="450"/>
            <end position="459"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="627"/>
            <end position="639"/>
        </location>
    </feature>
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            <begin position="640"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="800"/>
            <end position="824"/>
        </location>
    </feature>
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            <begin position="877"/>
            <end position="891"/>
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    </feature>
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        <location>
            <begin position="892"/>
            <end position="902"/>
        </location>
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        <location>
            <begin position="967"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1013"/>
            <end position="1025"/>
        </location>
    </feature>
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            <begin position="1056"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1121"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="1277"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="9">
        <location>
            <position position="932"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ84282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1545" mass="170678" checksum="C6E53F52FE7BEB66" modified="2017-01-18" version="1">MVLLKRKQSSSAGHSRKSSVQSLNSSAGSASSSSKKFSLATPTSSSLAQAHQIPSIGHGSTKDSHLQLNTDLKAEMVNKTSPGSPGSRLSSAPVFSGSKRLSLALRSLSSTSSSVNSTSGKNEEDSQLPASSATSQSSIINRSSHYASFKAQNGGNYDHSRSSKAASIAEKDSEAFKQDPVDSKLIFDWDVTNPKQWSSQRVVTWLQLNDFSSKWISFFRRQQIHGDAFLKLMAYDNFQRYENYLPSSHTSSYARFQHLLKRTMEQNVIHQQHSHSRSTGSIDSFQSRKSTKHSRTDSENSSLLSLYQQNQPPLKPLQKLDSSEQPLTKLPTNEKDGTTTATLSVAESDXKTQTHGLSSNSSNDNSTMPQKSNSKSTASLYRRSFISLRNSVPSGTSSTKVTPPVPPPRPNMAINPKIQDLSSSKESPIPSPTYGFFRRHQKTSSSESFLLSNKLKNGNNGTGPGYQSDLYDSRKNSNETLKTSNVSANSHADTSVLHETKKQQQTLSKLIGAKFCPKEDNKIKRKFVLVTKERSTFFPVEVSSITDVSTFQSKLANSSNIHHKNFQIYLSSCTFEIGETPLTKEVLNLLLKNNFSEGFDKFFVKDMLKIQLQRTRSSSLIKRKPSTRSATSSVKSSTTDDGFSTQTSGELASLEEKYNGQKAYPKTPNHYFENAEGTDYLTVKNEKKTASXVLDEAPPLPNNKSSFKLIRHDNTNKIDFNIKRETPFVKLEPKREAPKPPLTASGSSASINTYSAETPKMSKKSSFINATPNKKATPLKRPPPPVPFNANGEPVSFAQQNHKLPDNFSDSNISAFTPGSSQILVPQPYKGTVGESKDYPSYAYALSKKSSTLSLRKKSSTASLKTSDHLSTKLTRSNSSLQSSLNSVFQSPPRSLQRNPSRRVISSASAADVFEENTISFDDAPALTNAKEEFKDTNEEVFGSVDQKIVSSINQEPEMSFTVGDDMISESDDSEESDGIVWADAKDTKNSDSITKTVDDSDSDDGIVWTKPDATKSENKQKDSXTDACTKTSSNSEGKDSNDDDEEDDGLIVWKGQTQKDSSCKQVSSSEKTLVPEASKKGLSNDGITRKLTLRPSPEVVYQNLEMFFPGTDLDKPVLDGNSSPASPNFSDSQGFKRQSSKVAQSPLTSASTIQHSSKNDDYSFDTSNTLRSRDDNGKLKPPKRTKTIRMVAHEAREARKKSSNRKLKRNNTTKLWGTKVVEITDKENIVAINKKKNSKGEYKEFAWLKGEMIGKGSFGCVFIGLNITTGEMMAIKQVEVPKYNNNNESTNSVVEALKMEVSTLKDLDHPNIVQYLGFEEKNLVYSLFIEYVAGGSVGSLIRLFGRFDDDIIRFLTEQVLKGLAYLHEKGILHRDMKADNLLLDLDGVCKISDFGISKKSKNIYSNADMTMRGTVFWMAPEMVDTKQGYSAKVDIWSLGCVVLEMFAGKRPWSNLEVVAAMFKIGKYKSAPPIPDDTLPLISTGGKNFLNSCFEIDPEKRPTAETLLQSPAFKKAPAFDFSQTTLFKFINDNDKMNSSKMQLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBN0</accession>
    <name>A0A1E5RBN0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2661</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBN0"/>
    <dbReference type="InParanoid" id="A0A1E5RBN0"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ84291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="69" mass="7759" checksum="2B5DEDFF1799E713" modified="2017-01-18" version="1">MKEAREAIESQKAKNGNILEHLEHLEHLDDDDDDDDDDDDVIVVIVDIVDDATKAFLNNVYCVECSTAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBR8</accession>
    <name>A0A1E5RBR8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable cytosolic iron-sulfur protein assembly protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CIA1</name>
        <name evidence="5" type="ORF">AWRI3579_g2520</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for the maturation of extramitochondrial Fe/S proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with NAR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
        <text evidence="3">Preferentially localized to the nucleus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat CIA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBR8">
        <property type="interactions" value="77"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBR8"/>
    <dbReference type="InParanoid" id="A0A1E5RBR8"/>
    <dbReference type="OrthoDB" id="284782at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03037">
        <property type="entry name" value="ciao1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028608">
        <property type="entry name" value="CIAO1/Cia1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="111"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="157"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="330"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ84331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="346" mass="39212" checksum="701FDA6D8457F991" modified="2017-01-18" version="1">MLSLIKDLPVHEDKVWSLDIQRDNATGAPIFLASASTDRRIKLINLTNQKYETLETLDETVHKKSIXSVAWRPXTPKGNFVLAAGSFDTSISIWGKDTTDGNQDTELLALIEGHENEVKCCGWSHTGTYLATCSRDKSIWIWEADSYSEEFECVAVLQEHSQDIKHITWHPQQLLLASCSYDDTIRIWRPYDDDWECCAVLEGHEGTVWCSSFETLNDTETDTVRIVTCSDDSTLKIWKLIEDDFADGEDGCDEVEQTWEVETTLPSAHTRQVYSCEWNRDGRIVSTGADGKLVVYKETSPRVWKIEHEYSDCHGSFEVNCCKWVSNNQVATGGDDGNIKIWGISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBS0</accession>
    <name>A0A1E5RBS0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Isocitrate dehydrogenase [NAD] subunit 2, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2663</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBS0"/>
    <dbReference type="FunCoup" id="A0A1E5RBS0">
        <property type="interactions" value="908"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBS0"/>
    <dbReference type="InParanoid" id="A0A1E5RBS0"/>
    <dbReference type="OrthoDB" id="10261637at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF34">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="5">
        <location>
            <begin position="27"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ84348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="357" mass="38309" checksum="BA2267D0A4258630" modified="2017-01-18" version="1">MLSRSSTLLSQSLFKRTLATSAPGKFTVSFIEGDGIGPEIATSVKKIFSAADVPVEWEACDVGPIIVNNNTAIPEAAIKSINKNKVALKGPLATPVGKGHRSLNLTLRKTFDLYANLRPAASLKGLKTTYSDIDFVVVRENTEGEYFGVEHDLGDKVEAIKLITQNSSEKVIKFAIDHAKFLGRPKVLVVHKATVQPLTDGLFYQVGKSLEKYQSNVKVEFELLDDYIHNSVKDPKKYHDYVVVAPNLYGDIITDLNSGLSTGSLGMTPSANIGEKVSIFEAVHGSAPDIAGKDLANPSALLFAAVMMLEHLGLNSHADKIKAAALKTIAEGIKTGDLGGKATNSIYTDAIIKNLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBV5</accession>
    <name>A0A1E5RBV5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">Actin-related protein 2/3 complex subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2857</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Functions as a component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.</text>
    </comment>
    <comment type="function">
        <text evidence="7">Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Arp2/3 complex plays a critical role in the control of cell morphogenesis via the modulation of cell polarity development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ARPC5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBV5"/>
    <dbReference type="FunCoup" id="A0A1E5RBV5">
        <property type="interactions" value="515"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBV5"/>
    <dbReference type="InParanoid" id="A0A1E5RBV5"/>
    <dbReference type="OrthoDB" id="195498at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044396">
        <property type="term" value="P:actin cortical patch organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.190:FF:000003">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.190">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006789">
        <property type="entry name" value="ARPC5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036743">
        <property type="entry name" value="ARPC5_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12644">
        <property type="entry name" value="ARP2/3 COMPLEX 16 KD SUBUNIT P16-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04699">
        <property type="entry name" value="P16-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039096">
        <property type="entry name" value="p16-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69103">
        <property type="entry name" value="Arp2/3 complex 16 kDa subunit ARPC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00060329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ84384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="158" mass="17561" checksum="206E3B87D2D2981F" modified="2017-01-18" version="1">MEDWRRIDIDALDPENGRLTAADLTKPILQTGEPVYSSQDVQGIMGSLRSYASSGDFTSVVKLITAQPVYFADSEQTKDMYVKQVVESLAQCRTMEISKIVSGLTDSECDVLIKFLYKGMSIPEGAKQGTILLAWLDNIIEKNGSNSIIRYLTDRKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBV7</accession>
    <name>A0A1E5RBV7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Lactose permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2853</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBV7"/>
    <dbReference type="STRING" id="56408.A0A1E5RBV7"/>
    <dbReference type="InParanoid" id="A0A1E5RBV7"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000134">
        <property type="entry name" value="MFS sugar transporter protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF24">
        <property type="entry name" value="HEXOSE TRANSPORTER PROTEIN (AFU_ORTHOLOGUE AFUA_8G04480)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="183"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="229"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="345"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="383"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="412"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="454"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="485"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="512"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="80"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ84063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="583" mass="65205" checksum="D0D0F235A08BC49F" modified="2017-01-18" version="1">MSHLFQNNKNKNLGTGVHQNSSASSSFNDEGVMKNDENITSNLAADYQLSDAEVTIHTVMPSFDGKKWFQLKHIQKLCFVIFVISLTACNTGYDGSLLNSLYTMPDFNNAIGNVNGSILGALTNGYVFGCLLSFFVTAKINDALGRKKALIIGNVIMLAGVLVQSFSGAWIHGLPENYTKRDILGMMIGGRIILGFGSGILQVAAPSLISELSFPTHRQATTTVYNSSWYLGAIVAAWVSFGVKNLHHHWNWRIPTILQCLFAAAQICLVPFFVPESPRWLVSRDRIEEARDILNKFHGGYYPGSEMLVDYEMTEIQLAIEQERAVSSKSSYKDFIKTKANMKRLWILSWVAIFMQLSGNGLVSYYLGKVLNSIGYTSTSQQLIINACLMIFNYGVCIIQSFCIIPYIKRRWAFNGSLFGMLFCYVIWTVLSAINQQRNFEDKGLGKGVLAMIFLYYFFYNLGLNGVPFTYITEILPFTMRAKGMGLFVGIQFIVQVYNGFVNSIAMDAIEWKYYIVYCCILAVECAVCYFTFIETSGRTLEEVAEVFGDGIEDLAKVSGITALEDGKVKPDVEHKEYTTTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBX4</accession>
    <name>A0A1E5RBX4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2689</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBX4">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBX4"/>
    <dbReference type="InParanoid" id="A0A1E5RBX4"/>
    <dbReference type="OrthoDB" id="2420415at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061136">
        <property type="term" value="P:regulation of proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02666">
        <property type="entry name" value="Peptidase_C19J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044635">
        <property type="entry name" value="UBP14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025305">
        <property type="entry name" value="UCH_repeat_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43982">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43982:SF6">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13446">
        <property type="entry name" value="RPT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="8">
        <location>
            <begin position="758"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ84395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1229" mass="142950" checksum="BD66DBA1F1D60B49" modified="2017-01-18" version="1">MLLHTDTSPLDHLNQNTETTRNSLSDEEQVDENSKQVHRSESDEALYAGGDVEREVAVDDGKTNLYTTLSGSFPFKTSETLLDEILNDIFFIKEGHGILKEPTIAYAKKRDDLFNTTNYNTSLLYLNDQITVQSNYVYHSKACPDLNKCKITVAMAALYENSAQLHTTDDIENIKIFHLKITVKEKPQMQYKLKDIERFHLVQYTDLHEFDKQDLIVEPKCLDSAIFVSPVTNKVIMIEILDSEFSKQDLGTLKSKSILERYMNFYGVTEEEMCVDVGTAKEKEISNNNSETTNRKPIPSQLQCVYFLFKIIKGALNEESKEAKKKIIKESFPLNTQIDKNVLLENFHFHLVQEESQPLDQDEDGVVEIKEYYEAPDMFDYESNPSHRIIRDGFVRKALEAVFLGQIFHNIFPSPADYNKYFRFFLTSSSPMFWFNLIGQYVGNPNAFLDDLNRSKYYNLNYNFISLNAFKHYQPHTIINNFKRSIELDPENTAKYYDNLEFVASVVNNKQLFQYLGTLDIVGHEALKKAFALYDLSQESSVDEQEIYNTYKTKKALAGLSSDQNDQNMAASLKSCLRTLSNYVNSNFLRFLVEFEPYSTVQEAYKILNLETSINDYDWIQTAYMLKVSDQPALKVDADRALFTVAIYLKSLPLFTLLFEQEPRYQLYFNNVGTLTIEQAYKDLGINENCTDDTLIEVFKANWNAVNIELLDVNYDQLLKLRRAFCKVSTYRNSKILEIFVSTGKVDESYLPSGNWPVGLCNVGNTCYLNSLLQFYFTIVPLRTYIMQYSAKMETLINENGKRRIGGREVSAFEVQRSTKFINQLRTLFFDMIHTNSKVVTPSQQLVYLAFAPSEFIQQDSSLSNNTKDDEKGSDEMEVTQDQLELTLEMGRQQDVTECIGNFLDQFETVTLPLKLEKDFEQYDLIKKIFYGVLKQNFIKVQSREYIRDREREERFSSLLVNLGSKPRNLYAALDQFFNDEEEIEISGVGKVQRQLELTELPLILQVQIQRVEFDKTRYIPIKNIDPLPFDEQIYMDKYVTNGVPLQAKEEYETKKSQLSKLKERQSQMLARNEVGQSVKNAISQTRKFLETDFIQTEMNMDXQELKETTTYLSQVQDEIEEKIKKLGLEISLLEKSIDVHFQHYQKHPYSLFAVFVHRGEASYGHYWIYIKDFEKNIWRKYNDETVSEVPESEVYNFTEGNTATPYFLAYIQDDHQMEIEPLKRALDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RC30</accession>
    <name>A0A1E5RC30_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein PRY1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3099</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RC30"/>
    <dbReference type="STRING" id="56408.A0A1E5RC30"/>
    <dbReference type="InParanoid" id="A0A1E5RC30"/>
    <dbReference type="OrthoDB" id="337038at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184684" evidence="1">
        <location>
            <begin position="20"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="2">
        <location>
            <begin position="76"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="221" mass="25222" checksum="F7287CA28BC4D3FF" modified="2017-01-18" version="1">MITLLQYFYALLFVKITFAQSSTFAEGNYSLETKTVTVTSVDTEYRTYRFTKYTTKTVPYVEVPTTLPSVQQVDSLFKAKALNAHNALRKLHNVPSLVWSDELYAKAQSFANNEFICNGTLNHSLDFKTETVGENLALGYDEIEQAVDAFYNGIKFYNFNSPQTQDQKSAHFSQVVWNATESLGCGFISCGDYYRKFFICQYAQRGNIFGEYKTNVFEPVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDK3</accession>
    <name>A0A1E5RDK3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1747</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDK3">
        <property type="interactions" value="113"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RDK3"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="36"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="225"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="271"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="45"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="354" mass="40498" checksum="146CA601F239DB63" modified="2017-01-18" version="1">MSHNDQIYPNVGGVRGNGRVYNGGYGNSELLDGAHNDGFHHYDSDEYASDENEDEDEDENEYTGKEDNIFKWTLDALLGTRFQKGKKDKVDNLKNENYAGTKIQAGNNLPFGNINENESKLSNFSENLFQTQNDDMRAKFGLDGGLKDTDEITNTFNLKGDYNTNTLNFKPLRSARKDDMAPAYTNFGVKAGENLKSSKDLFSXHYYPDGENFNQHNKPNANAIPGGFGDPTSLKKTTNHSRQLRDETPPRINTYKQKDNSQKPSFINKPGKNQENIMAELENNNAKLSKILETREAQEQQEMMQEQSYKKKYTHLKKNYKSLLTSYRDLSEVAIQEIQNLKMENDMLRQQLNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDM3</accession>
    <name>A0A1E5RDM3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulatory protein RXT2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2155</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDM3">
        <property type="interactions" value="165"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDM3"/>
    <dbReference type="InParanoid" id="A0A1E5RDM3"/>
    <dbReference type="OrthoDB" id="2405722at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039602">
        <property type="entry name" value="Rxt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013904">
        <property type="entry name" value="RXT2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28232">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN RXT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28232:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN RXT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08595">
        <property type="entry name" value="RXT2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcriptional regulatory protein RXT2 N-terminal" evidence="2">
        <location>
            <begin position="97"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="500"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="507"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="522" mass="59136" checksum="B06ECD5FA5EC474E" modified="2017-01-18" version="1">MSTLTEQQNTSLTETSTSXDQPGTLNNSIPENNSIPENNNNMASIDEQSKGEVLDEEKLISKFTRHVIKQRSGNFPEVKRRTTDGCIYPTVCSNETTNRGRKLLQGSEWVSRGKLNTSLYENRVFYNGVEHKLLSRKRQKLNPTLTNTAEESIKXQQADTDNDNNNDDGDMTDDDFDLNQIVNVEDALKPISSLGDVVTHPTVKKTFLNKKVLNNLEQQTTLMIEKAQMSSTAYSDLLNVFLGDDPLPYLCDKMDLPVYDHNLHIDESHEYIDISKEQLDTVQIGQDESKLIGGKIKESTFGSQETVQKISKDITPETQPDQGKAKQQTEKEQEEQEEEDNNKDTIEEIEAEQSSNDIDKPTADSKITKIEENMNTTSQDQTLATGAADDNVDEIDDPFFALPRYKTNTLLERMNDLQKQGLYIDDIENARQLTQIALQRNQEFIKNLTRIRMNILKAERIRTRLVKWGQEYAGIPEEGVSVPNILTVVKRGLISASTNNISQGESLDQEDQDEKETEEENP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDX4</accession>
    <name>A0A1E5RDX4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Verprolin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2365</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDX4">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDX4"/>
    <dbReference type="InParanoid" id="A0A1E5RDX4"/>
    <dbReference type="OrthoDB" id="2430277at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22064">
        <property type="entry name" value="WH2_WAS_WASL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH2" evidence="2">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="428"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="120"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="270"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="443"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="500"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="525"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="544"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="554"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="612"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="657"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="681"/>
            <end position="713"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="767" mass="75714" checksum="3168CCE955886580" modified="2017-01-18" version="1">MPPPPPPPPPPPMGGPPAPSGKPSMPVVTGDRNALLGDIVKGKTLRKAVTNDRSAPTVGSGGVVSSSHSSQAIGSGAPPVPSSAPSAPKPPPSEALGGAPAGLAAILAGGLPQLHHRPTEQASSTSASSTPAPIPPVQSAPEIPSMSAPPIPQMGAPAIPALGASALSTNDLKLSANAPTAPRRPTNRPQKKSFAQSPAAPSVPPVPTMSAPPAPPLPNAFSSQAPPVPNLSAPPLPNAGAPPPAPPLPGTVQKPEATSIKMTSAPPAPASLAPAVPNVSAPPTAPPLPATSAPVAAPPAPPLPTTAAPPPAPPLPNGAVNLDSKTTKATSAPSLPPAPPAPPVPSFTAPPAAPPLPKANAPPSAPPLPGNLSSSIPKAPSPPPAPPMDPPVRSTKSSSAPTAPVASGALPFLAEIQKKRDDRYVVGADTGYGTVNAPSQTDSTAKTHAPASAPSSAAPPLPATTGNSFLDEINSKMKNKHNAAAPSIPAFNNEGASSAPPVPSPAAPPLPTMGAPPTPALPSSFAPTIPTSPIPTQKPKVSLPSPPPAPPLPQLQAPAAAAPSVPVAAAPSVPVAAAPSVPVAAAPPLPSVSAPQAPSMPDLSAANEPSVPFSPPITPAPPASVPPVQSAPPATSFQQDSTAASHKSPPPPPPPTTATTSPVPSRSSTVNGSFKHRLFSSEENTSASGTXEQPSSDAFKNPQTYTINGSEKTSGVKLSGEKVVIEDSKYKFVNQFELPAPRKFSNRTKLYPSGRGSSVPLNLSLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REI8</accession>
    <name>A0A1E5REI8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">glucan 1,3-beta-glucosidase</fullName>
            <ecNumber evidence="9">3.2.1.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1791</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1-&gt;3)-beta-D-glucans, releasing alpha-glucose.</text>
            <dbReference type="EC" id="3.2.1.58"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.58" evidence="9"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REI8"/>
    <dbReference type="FunCoup" id="A0A1E5REI8">
        <property type="interactions" value="153"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REI8"/>
    <dbReference type="InParanoid" id="A0A1E5REI8"/>
    <dbReference type="OrthoDB" id="62120at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004338">
        <property type="term" value="F:glucan exo-1,3-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000033">
        <property type="entry name" value="Glucan 1,3-beta-glucosidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018087">
        <property type="entry name" value="Glyco_hydro_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050386">
        <property type="entry name" value="Glycosyl_hydrolase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297:SF1">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE I_II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297">
        <property type="entry name" value="GLUCAN ENDO-1,6-BETA-GLUCOSIDASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00659">
        <property type="entry name" value="GLYCOSYL_HYDROL_F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184717" description="glucan 1,3-beta-glucosidase" evidence="11">
        <location>
            <begin position="20"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="12">
        <location>
            <begin position="104"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00036824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ84973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="445" mass="51534" checksum="B5922BEC6A3CC49D" modified="2017-01-18" version="1">MQFSSLLPLVPLFASLAAAAPVFKKHENDIQYYTKRNYMDYDASLIRGVNIGSLFVLEPYISPSLFEPFYDETKPAYEDGYSYGIPVDEWHYCEALGYDECANRLEAHWDSWYTEQDIVDMASMGINMIRLPIGYWAFQLMEGDPYVTGKQEYYFDRIIQWCGNNSINVWVDLHGAAGSQNGFDNSGLRDSYGKFQTEEYMNVTQSVMKYMLQKYSAEPYVDTVIGIELVNEPLGPILDMDLLKDYYQFGYDYTRNDLQSNQILIMHDAFQPFHYWDDFLTIDDGNYGIVIDHHHYQIFSNSEVQRDIDEHIEVACSWGTNIKNESHWNVAGEWAAALTDCAKWVNGVGFGARYDGSYVNGDTTSSYVGTCEGINDISTWSSEQIENQRKYIEAQLDAFDQRGGWIIWCYKTETLLEWDFQRLVYNGLFPQPIDDRKYPNQCGFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REV3</accession>
    <name>A0A1E5REV3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2360</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REV3"/>
    <dbReference type="FunCoup" id="A0A1E5REV3">
        <property type="interactions" value="930"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REV3"/>
    <dbReference type="InParanoid" id="A0A1E5REV3"/>
    <dbReference type="OrthoDB" id="10263513at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004977">
        <property type="entry name" value="Ribosomal_eS25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12850">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03297">
        <property type="entry name" value="Ribosomal_S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ85093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="108" mass="12082" checksum="46C19D03C95CC85D" modified="2017-01-18" version="1">MPPKQQVSKAQKAAAAMAGGKKSKKKWSKKSHKDRAQHAVILDEEKYERILKEVPTYRFISVSVLVDRLKLGGSLARIALRDLEGQGIIKPISKHSKQQIYTRATASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFA8</accession>
    <name>A0A1E5RFA8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA damage checkpoint control protein MEC3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1984</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFA8"/>
    <dbReference type="FunCoup" id="A0A1E5RFA8">
        <property type="interactions" value="204"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFA8"/>
    <dbReference type="InParanoid" id="A0A1E5RFA8"/>
    <dbReference type="OrthoDB" id="419537at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030896">
        <property type="term" value="C:checkpoint clamp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044778">
        <property type="term" value="P:meiotic DNA integrity checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007150">
        <property type="entry name" value="HUS1/Mec3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12900:SF0">
        <property type="entry name" value="CHECKPOINT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12900">
        <property type="entry name" value="MITOTIC AND DNA DAMAGE CHECKPOINT PROTEIN HUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04005">
        <property type="entry name" value="Hus1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="370" mass="42244" checksum="77D923ECD4F17217" modified="2017-01-18" version="1">MRLQFHINSQDTSKEFSCFLKGLVLANSIRKSAVFKFKEDELLIILTSGSSSPMSSNTGSSSQSDSQLWLKINTDPLLQSYVIKTKRVDLSISMELALQPLIQILRQYDQMMKRGDQAHTSAQTMGNLEIKMMNVPKEWNLLILGGNLAQGNRSGSGQDNKLKNSGYGSLRVSFEEQVPGKPTPIKHVTFIPMKLLSSQYQLRQPVINADQIVYNLPQQNDMDEGKQFGKFMKRLDRFSGLEHLKVCGTTQELKLVVDEMDWFMEVKWNGVIDVLNRESMTQNEEERIANEEQMVIIRAKDWKMCTKLFEVLGEQTFLCISNKQYCLFNCTIKNTDSMDADTLSQSHDSLADQGNRFMDISFYISNCKSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFF0</accession>
    <name>A0A1E5RFF0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2388</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFF0">
        <property type="interactions" value="166"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RFF0"/>
    <dbReference type="OrthoDB" id="4063618at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="unsure residue" description="I or L" evidence="1">
        <location>
            <position position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ85586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="321" mass="36004" checksum="5E7FF5B10AC28245" modified="2017-01-18" version="1">MDLYENLLANDPQIGFSATAINRYKRSLSSSSVEKSGNGPSSXEAKLVKKTRHNILAIDENNKVNYENIRGVPGSFHEYDLHGLTENSGNSSSVNANHNNNAKVDTPVGEASPSDKKVITALNEPSDGNARTMCDFESEKKIVAKLSQTKTRSLKSYLSNQNNTMCTNIEHLNEKYSTEEASVPRTDEMDDQNLQSYLNDLQELQKKLLDEYKYWKNKETKMFLKKELLLDANLKVDIFAKPTSLTETLQLEIHEKVNAIMHPSINDSLLVAEDTDNDDNSTDATNKAKTKLSKITKNKKVVPLALDEKKLAKSECTLLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFH2</accession>
    <name>A0A1E5RFH2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Chromatin structure-remodeling complex subunit RSC1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2073</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFH2">
        <property type="interactions" value="365"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFH2"/>
    <dbReference type="InParanoid" id="A0A1E5RFH2"/>
    <dbReference type="OrthoDB" id="1742084at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04717">
        <property type="entry name" value="BAH_polybromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037382">
        <property type="entry name" value="Rsc/polybromo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16062:SF21">
        <property type="entry name" value="CHROMATIN STRUCTURE-REMODELING COMPLEX SUBUNIT RSC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16062">
        <property type="entry name" value="SWI/SNF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00439">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="10">
        <location>
            <begin position="27"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="10">
        <location>
            <begin position="467"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="BAH" evidence="11">
        <location>
            <begin position="569"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="138"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="247"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="722"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="835"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="917"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="258"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="291"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="926"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="12">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ85651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1094" mass="125005" checksum="41D2DA9BADF99FFC" modified="2017-01-18" version="1">MSALTDKLKTLLEKFLEELPPALVAEFTTLPPRQTFQHYYMTIKKPMSFMKVQRGLKNYQTAQSFLNDVCQITWNAKFYNSEESLLYQYANTIEEKIKTFIIPALQILDSTVYFPHLGNLPGEPDDVQIVDAMSTSNEAXSSEAVTKNENAEQXSLPSKGTVDLSSDHTNDNSHAVNNTGDLKQAINEKVITSSSPLNQSVVHASPLSMQNQPMMNXNDHHNMAMSAAAPIQKPVYHPNPYVSSYPQQFPTNXPMPLPMQHMPHSQSXPSHQQQPVMNXPYSPGHYQKQHQPQQPQQPQQPQQPQQPQQXQQPQQPQQXQQPQQPQQXQQPQQPQQPQQPQQPQQPQQPQQPQQPQQLQQPQQXQQPQQPQQPQQPQQPQQPQHLMVPPAHGISGGSQVSSLSSYYLNQSTDSGFVYKYVPEHVTHLRNLNEPRKKGRPSIKKFIEQEFLYVLKHLKRVNENGYKVFNELPPRAEMPKYYEAITRPVSLESIKHAIRKNYVRTTPQLKEFFQLMIDNHKLYYASDYNQIMLANSFSQDFLSALNQYEREEQTEGQELREALDKVEYNGNYFEVGSWVLLYNRVNPSLPIIGHIFNIWKESDDNTVWISVSWYLRPEQTVHRYDRTFYYNEVFKSSQTRIHPMEDIIGKCYVVFFTRYQRADPLEREGPLFVCECRYNDADKKFNKIRTWKGCIPEVCREIPDLDAPVSNRVFLKKESPLRYKLPPNAQPTATPPSLDDTREEMQPSEGNVYIGLPLTKDDLGEYFPSRFETPKYVIQPGQPIPPNVVINHETGTLSDPNGPTVNTLSHKSSNRLASLNNMPVRNVSNSDLSAAARNRFPTAGGTPMSGASPAPQMLIPPNLLQQQQQQQLLLLQKQQQQNQAQMLAQSRKNFSLQQQLQRNVPAMQTMPGAQRVANRVNKLPNRTSGSTHKSKINSSSSVSRNSHKDKHRDTNSQKAKLQVAGRGVQVEPMANRGIAPFLKGREDKVVLDSSASYVLSDVLDKNIEPVARTDLLNQQKLLEMNPGLENNSTALGRVGKLRGDIIWFRGTSSKVNRRLLDSKKFLDVYDDLKASPEYLAWKIKQQENLETSESFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFI2</accession>
    <name>A0A1E5RFI2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">ATP-dependent bile acid permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g2296</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFI2">
        <property type="interactions" value="124"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFI2"/>
    <dbReference type="InParanoid" id="A0A1E5RFI2"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18596">
        <property type="entry name" value="ABC_6TM_VMR1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18604">
        <property type="entry name" value="ABC_6TM_VMR1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03369">
        <property type="entry name" value="ABCC_NFT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000825">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF353">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN_PERMEASE VMR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="289"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="400"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="448"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="598"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="626"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="705"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="736"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1137"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1246"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1347"/>
            <end position="1364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1429"/>
            <end position="1452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1458"/>
            <end position="1478"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="414"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="798"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="1149"/>
            <end position="1486"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="1522"/>
            <end position="1799"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="104"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="499"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1070"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="108"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1099"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="14">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ85655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1821" mass="207084" checksum="91C5437FF0407E57" modified="2017-01-18" version="1">MDNPLISNNQEQNSLDLDTFLYKNHNSTIGNPQNGTDGHIPRHLGRCNLKSGFWNFDDLTDCGRIIDLHYYLPIVLILFNVLFLTYKIVYYLKKKHDLKKIEQINTPNNHNNXNNHNXNNNHNNNNNQNKNDINGTSNEDENSPLLNNLKNNTKKQTLRDQHFSIFKLNMDHKNHKEVIFKRDFKEYSRLILEWILVAFQFVYHLMILNRYSNKIADFPLKVTQINCILFTLLFVNVTIRLFWNFTNRNELINKXRLWTASFVTYLWLFIGSILPFRSGLIHHLQYSKIVFIYYNLQFVITTLLLILLATSPIKNELPVLYETGDSKMNVDGYYCIPSPEPKTSILQFISWSFFDKFVWLSHLKTIEMKDVWSLSKEDHSLNVLKNFKNFYKNEKKFSKYLILFFSKYLLLQGFWAMFDSIISFMPTVLLQRILEYVSDQKSTPKNVAWFYVFSMFACKMLAAIFSGQALFFGRRVCIRMRSIIISEIYTKALRRKISNSSTSPTKENEEEEEKLKLGKSTNSSKEQQQQQQHEQEEAQLLAADMVAQEIINNGEWTDPQAINDAIDIDADEETIEESANLGAIINLMSVDAFKVSEICAYLHGFVAAIVMTIVALVLLYRLIGMAAIVGAVLIVVMLPINFKLATLLGXYQEKSLGITDKRIQKINETLQSIRIIKFFSWEENFIKEIQDVREQELQMLIKRSVVWALGGLVWYITPSIVTSISFAYYIYVQKEVLTTPIAFTALSLFALLRNPLDQLSDMVSFVIQSAVSLRRIQKFLNEEETQKYQQLTVSQDKLGFDNATISWDAANNDFKLSKLNIDFKLGKLNVVIGSTGSGKTSLLMGLLGEMDLVDGKIYVPNLDPKQDLIIDSKNGLTNSIAYCSQAAWLLNDTVKNNILFNSEFHPIRYQQVIEACGLKRDFEILAAGDLTEIGEKGITLSGGQKQRVSLARALYSTSKHLLLDDCLSAVDSHTALWIYENCITGPLMENRTCVLVSHNIALTLKNAEWVVILENGKVKDQGEPLELLRNGALGNDEFIKTSILTKTTSSINLAAKNAVANATATVATSGKNLDDKHKKEKKGVDLSTLANTDNNDLENGEHKNGPKIDDKAKKELGKLIEEETKSDGVVSIDVYKWYAVVFGGLKIILFLVFVFVIANLSNIIQSWWVRYWAAHNFVAEIINKFSTMSGAYYSSFLMPLKVTYDSSASLSSFLSNDDIVSKTMMNSSSNAISISETTGHSTKYYLLIYFGIGCLAAFISASKTIISFIAGINAARKMFNIVLGNVMYAKLRFFDSTPIGRIMNRFSKDIETVDQDLTPFMESSFISLVQCVSTIILXAFITPSFLFGAFIVCFMYYLLGYFYICGSRELKRFESITRSPIHQHFSETLVGVSTIRAYGDERRFMQENLDKIDENNKPFFYMWVANRWLAFRIDLIGALVILFSGISILVQIDSIDAGLAGISLTYAISFAESALWLVRMYSNVEMAMNSVERLKEYTDIDEEPLNKDGYIPPKEWPEKGNIEVHDLSLRYASNLPRVIKNVTFTVESNSKVGIVGRTGAGKSTIITALFRFLDPETGFIKIDGVDITSIPLKKLRQSITIIPQDPTLFTGTIKTNLDPYDEYNSEEIFESLRRVNLIDAQELDILKKNNFVYPASLLNNIDNDVVANSGEREAEEEGEDINDNVNKFLDLSNKISEGGXNISQGQRQLVCLARALLRSPKVMLLDEATASIDYNSDAKIQETIREEFSNSTILTIAHRLRSIIDYDRILVMDAGEVKEYDHPYSLLLNKESIFYSMCEDSGELETLIQLAKESFVKKLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFI5</accession>
    <name>A0A1E5RFI5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein N-terminal amidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2187</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFI5">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFI5"/>
    <dbReference type="InParanoid" id="A0A1E5RFI5"/>
    <dbReference type="OrthoDB" id="201515at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008418">
        <property type="term" value="F:protein-N-terminal asparagine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070773">
        <property type="term" value="F:protein-N-terminal glutamine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039703">
        <property type="entry name" value="Nta1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11750">
        <property type="entry name" value="PROTEIN N-TERMINAL AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11750:SF26">
        <property type="entry name" value="PROTEIN N-TERMINAL AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="1">
        <location>
            <begin position="9"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="2">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="414" mass="46753" checksum="8DE63B876543B6CB" modified="2017-01-18" version="1">MNTNSMLKLKIAIVQLNPQIGQLKQTEKRAWSLLQNLKKENKRPDLIIFPEFALTGYSFHSRPQIDPHLEDVHKASKNAQTQHNLAFAASVAKDFECYTVLGYPEANYHNSAMVVSPSGQVLHNYRKHFTYDTDDEWNCVPGAGFTSFXLSLPVPKTTDSSNSPVVKCSIGICMDMNPDKFDDXKFNNFDFSSHCVDNDVQLVIVPTAWLHALSVTKRTEAKEKQAGIVKIKNLVKKFPYLQDPNKALANDVRVDIQNGPTVLQENSTSKEQSNAIADYDPSKPDKSTVDYWMLRFFPFLYNPNRSSWFEFLQENRYLQRTGRNSYLGVTDIKEWGFEGKETVLCICDRTGVEEDSTVYAGSSGIYKFNGKSFPEHGAKAEETGVSHPGVEVYGILPQGQEGLLYREVELNVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFQ8</accession>
    <name>A0A1E5RFQ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16 17">Ketol-acid reductoisomerase, mitochondrial</fullName>
            <ecNumber evidence="5 17">1.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15 17">Acetohydroxy-acid reductoisomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14 17">Alpha-keto-beta-hydroxylacyl reductoisomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">AWRI3579_g2068</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-acetolactate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22068"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-ethyl-2-hydroxy-3-oxobutanoate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13493"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49256"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="17">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17 18">Belongs to the ketol-acid reductoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.86" evidence="5 17"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFQ8"/>
    <dbReference type="FunCoup" id="A0A1E5RFQ8">
        <property type="interactions" value="740"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFQ8"/>
    <dbReference type="InParanoid" id="A0A1E5RFQ8"/>
    <dbReference type="OrthoDB" id="10255643at2759"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00056"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004455">
        <property type="term" value="F:ketol-acid reductoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000003">
        <property type="entry name" value="Ketol-acid reductoisomerase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000005">
        <property type="entry name" value="Ketol-acid reductoisomerase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000013">
        <property type="entry name" value="Ketol-acid reductoisomerase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000167">
        <property type="entry name" value="Ketol-acid reductoisomerase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013023">
        <property type="entry name" value="KARI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000506">
        <property type="entry name" value="KARI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013116">
        <property type="entry name" value="KARI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016207">
        <property type="entry name" value="KetolA_reductoisomerase_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00465">
        <property type="entry name" value="ilvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371">
        <property type="entry name" value="KETOL-ACID REDUCTOISOMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371:SF1">
        <property type="entry name" value="KETOL-ACID REDUCTOISOMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01450">
        <property type="entry name" value="KARI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07991">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000119">
        <property type="entry name" value="Ilv5_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51851">
        <property type="entry name" value="KARI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51850">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 17 18" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="13 17 18" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="21" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8 17 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 17 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9 17" id="KW-0521">NADP</keyword>
    <keyword evidence="11 17 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="KARI N-terminal Rossmann" evidence="19">
        <location>
            <begin position="66"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="KARI C-terminal knotted" evidence="20">
        <location>
            <begin position="256"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00072637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OEJ85714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="404" mass="45034" checksum="CACA8B0D079FF62B" modified="2017-01-18" version="1">MAFRTNAARMICNSRVIASKRAMSLLTKASTAPMLRTNTFGASATAAFKPLVASRGIKQINFGGSVETVYERADWPREKLLEYFKNDTMALIGYGSQGYGQGLNMRDNGINVIIGVRKNGASWKAAIEDGWVPGENLFDVKEAIEKGTIVMNLLSDAAQSETWNSIKPLLTKGKTLYFSHGFSPVFKDLTHVEPPKDIDVILVAPKGSGRTVRSLFKEGRGINSSYAVWNDVTGKAHEKAQALAVAVGSGYIYETTFEKEVNSDLYGERGCLMGGIHGMFLAQYEVLRENGHSPSEAFNETVEEATQSLYPLIGKYGMDYMYDACSTTARRGALDWYPVFKDALKPVFEDLYESTKNGTETKRSLEFNSQPDYREKLEAELQIIRNMEIWRVGKEVRKLRPENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RG60</accession>
    <name>A0A1E5RG60_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2277</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RG60"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="13"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="226" mass="26531" checksum="9B195199003393D8" modified="2017-01-18" version="1">MKSKNPIDVEAKCPICLDHLYNTCEKKYVAQIKPCGHQFHFNCLDTWLKLKQKQECXHSNRLPAVSGNKDAKCPYCRTSGDYIDRLKYDIIKQTTGLXPQICNVRXSKRVXLTFEYIWNTTISSNTSAGTAIASAGXEXNKMICDFTRSFYLMNMEKERKYEINLVVRKILDXLYHEHKKINKKQYIEINKKVSRQLYNCERENHKLFAKKLCEKEINHLFNKAKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RG75</accession>
    <name>A0A1E5RG75_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MIOREX complex component 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1982</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RG75">
        <property type="interactions" value="110"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RG75"/>
    <dbReference type="InParanoid" id="A0A1E5RG75"/>
    <dbReference type="OrthoDB" id="276721at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051207">
        <property type="entry name" value="ComplexI_NDUFA9_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126:SF16">
        <property type="entry name" value="MIOREX COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 39 KDA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="255" mass="28918" checksum="F205AE483BEC38F9" modified="2017-01-18" version="1">MKLPKVVVFGGHGFLGSQIVKQFPKNRYQLISLSRSLPSSKIAHVQYLPNIDVLKPETYDHIIKDADHIVHSIGVIFPNEAYKKIINSKSICDTFTGVCNYLTSNSSATSEKNKEXYHKINYESVKVLSEAFVSHLRKRSIGSXEVKPSFTYISAEETPMLKTFISPEYILSKRKSELQLLQYENDLRPIIVRPGLLYDEQARELTPRSSFAFLMKNVNPFANQFMCSTQRVAKAIVNKVSNPDYKGGITTLNEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGK1</accession>
    <name>A0A1E5RGK1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">G1/S-specific cyclin CLN1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1586</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGK1"/>
    <dbReference type="STRING" id="56408.A0A1E5RGK1"/>
    <dbReference type="InParanoid" id="A0A1E5RGK1"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044843">
        <property type="term" value="P:cell cycle G1/S phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044772">
        <property type="term" value="P:mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20559">
        <property type="entry name" value="CYCLIN_ScCLN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000010">
        <property type="entry name" value="G1/S-specific cyclin Cln1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 5" id="KW-0195">Cyclin</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="7">
        <location>
            <begin position="61"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="191"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="200"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="535" mass="61838" checksum="9DC0B2A43E450D36" modified="2017-01-18" version="1">MLIHKEKLCFAENTLKPYSTDLMAHYHYLNHYDLPDPHMLDMQPEINTSFTDDALRSQHLDFIINLHSFLQLKQETLYLTVLLHDKYCSKRIVYKKHYLLVMLSALWIASKYVDKKSRIPNLKELCYYANNANLKLDHTSIEYDNSMFIQMEQHICTTLNWEIGTGISQEEVLQIIFMNISNISNVSSEARDERELKGVQNTHDTQNTQNTKNTQNTQDTQDTQNTQGSNSSPQYSSHDILKLYQIASFLCEYSLYFKNFMFFNKNIVSCVSVLLASKIYNVHCDFNYAWMCIPNTSCVSELETILLDPFFESETDLNELRKCCHCFLNEIFNIQHNEKAKILFKKYKLINIMDLLHSFIENNYNAYLKLGFLSEKLAIVQTHPQNINDFPSSSSSSSPNTELNENHLNLQIISITDYFMNIPAFLYQHDKWSASLISDMAQNTHMDTVLDYQDQYSNYHNTPRAHCNDSPNQHSTSSSVFEYSSAISSASAVSSVSSQKAQTPMTPMSTMFMMKTLAHHHQRADTMNARSSGAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGL3</accession>
    <name>A0A1E5RGL3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1361</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGL3"/>
    <dbReference type="InParanoid" id="A0A1E5RGL3"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="86" mass="9645" checksum="2C5FA4843D0D28D0" modified="2017-01-18" version="1">MNPTYIRQIAKQSVRYNQVLNAAAHMNPVHETVHTHGMSPKAWFGVMGGVGIFFGGFVGFLDWCYTDSKMEGIFKNQRVQGASHSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGS3</accession>
    <name>A0A1E5RGS3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone acetyltransferase GCN5</fullName>
            <ecNumber evidence="3">2.3.1.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Histone crotonyltransferase GCN5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AWRI3579_g1060</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetyltransferase family. GCN5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGS3">
        <property type="interactions" value="523"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGS3"/>
    <dbReference type="InParanoid" id="A0A1E5RGS3"/>
    <dbReference type="OrthoDB" id="1937912at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140671">
        <property type="term" value="C:ADA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010484">
        <property type="term" value="F:histone H3 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05509">
        <property type="entry name" value="Bromo_gcn5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000046">
        <property type="entry name" value="Histone acetyltransferase GCN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000004">
        <property type="entry name" value="Histone acetyltransferase KAT2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037800">
        <property type="entry name" value="GCN5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750">
        <property type="entry name" value="GH11602P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750:SF3">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8 15" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 19" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="18">
        <location>
            <begin position="270"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="17">
        <location>
            <begin position="514"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="54"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="108"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="54"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="122"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="186"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="225"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00078361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OEJ86112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="611" mass="69849" checksum="05C872C84A9EC7A0" modified="2017-01-18" version="1">MAKKRGSHLAALNRRKKLRRLNEGLKLEEXSSISKNDVQQTGISKIXDQKKDTIAIKSEDGMRKVDQPTDQDVAETERHASSXPKNATVDDTXKGPDTVEIEAAKTLQQELDVDNINKELINGSSTSSGANKQTFDQPEITPIDVKREDDSELQPVSKDFEKLSSREKESELEQAKHMEEDLNKDNEEEEAIDVEADNEDQANDAAESLQPTSVVVSDKKXAKSTSQESEQVLSQVEVDESTGTTTFEFDGIKLKYKERSSVLEEKKGIIEFRVVNSDNSKENMMVLTGLKNIFQKQLPKMPKEYIARLVYDRSHLSMAIIRKPLTVVGGITYKPFNDRGFAEIVFCAISSTEQVRGYGAHLMNHLKDYVKLTSPIKYFLTYADNYAIGYFKKQGFTKEVSLDQKVWMGYIKDYEGGTLMQCSMLPKIRYLDAGKILLLQEAAIRRKIRTLSKMHIVRPGLEHFRNMDNITPIDPLDIPGIKESGWTPEMDELAQKPKRGPHYGPMQSILTELQSHNAAWPFLQPVNKNEVADYYEYIKEPMDLSTMETKLENNKYETFEEFIYDARLVFKNCRAYNGENTSYFKYANKLEKFFNNKVKEYPEYADLVESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGY7</accession>
    <name>A0A1E5RGY7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pre-mRNA-splicing factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1228</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGY7">
        <property type="interactions" value="902"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGY7"/>
    <dbReference type="InParanoid" id="A0A1E5RGY7"/>
    <dbReference type="OrthoDB" id="4822at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071008">
        <property type="term" value="C:U2-type post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045211">
        <property type="entry name" value="TFP11/STIP/Ntr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23329:SF1">
        <property type="entry name" value="TUFTELIN-INTERACTING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23329">
        <property type="entry name" value="TUFTELIN-INTERACTING PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="3">
        <location>
            <begin position="43"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ86126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="694" mass="80619" checksum="A635EE2570341C78" modified="2017-01-18" version="1">MISFKRKRNDSGXDVQATKQEKQPAHQTGTSTLSGPSLQLSSKYGIGAKLLLGMGYVPGRGLGSDGAGIVNPIETETRAKGVGIGMFRGMHPRSAGYDDDMYASSSDEEDLSTEQRALSSKYQQPVTFSANVQILKSSQEDELHLIKQRLQKLNYKYSEDELSIENLPKLKVLILRLSQKQAALAKAQQRHETLVNEQRTKQESLDAIDRLLEFLSSEQTTSKTNIDHXNIMHVIDETLNCSFFSNFEKDKYLSLLLDMHAQTINVDLEYLLQENNALLAMLNQVISLVAYHPEFQNSLAYDVQDHSDLSFLQSSIYFLLSPSFEQFFQNLIIPSDLSTCVTLLLDYEDVLKFINYFDYLVAQHLSRKLIQSLKTHDLLENHQLLTFFKDFELILXDMMKVLLKDIVKEQFQEFLQTWYHSDVSEFPNDLLEIIKSDVYGDPCVDFYRDITKSEFXQRFFEQMWPKYFDLQQDLVEGQEKFVLYFVKTLFQWKTLIEEESFNIILYHCFNEIKKVIFQWYFFKRDTFHTEAMGWYEWIKSVFPAKNLYCKQQLFDIDQWLKNGPSGCIHDETRNLYDDLFSKAETYNQNYTVXNIPLGKIECSFAEVVDKFCQEHDLLMXKRTAVCEPVLLEGVDAKEVHPLYVIQSLDSLGTNEIFEVLCLIKNDTLWIQSQDSANCDYFKPIILQDLLAYFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH27</accession>
    <name>A0A1E5RH27_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Bud site selection protein 22</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1302</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH27">
        <property type="interactions" value="263"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH27"/>
    <dbReference type="InParanoid" id="A0A1E5RH27"/>
    <dbReference type="OrthoDB" id="3364872at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037393">
        <property type="entry name" value="Bud22/SRFB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015158">
        <property type="entry name" value="Bud22_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23325">
        <property type="entry name" value="SERUM RESPONSE FACTOR-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23325:SF1">
        <property type="entry name" value="SERUM RESPONSE FACTOR-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09073">
        <property type="entry name" value="BUD22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bud22" evidence="3">
        <location>
            <begin position="61"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="203"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="205"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="286"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="396"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="420"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ86166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="498" mass="58207" checksum="35EB2268FA8A247C" modified="2017-01-18" version="1">MPKENLIFKLDNLEYQYHHLNNSLSSFDPRLNTTRKLFTLKGKNNIKNLDKKLSSTTLAEVETKLNQLKHQITTNKIYHLEKKLFLNLEKYYTAQGTKSSDSPLSLSTYKHITKSKIIKLCMNKLLNSKLLKKEPPTWVKDLDIYISFTDAQNLDNPSYVYKTYLSNNKEANKFFSKFVGDKKQIKLFKEFENGMDVFLNINKGKHGDQQEEQVPETKSKSDRNVNNKEDEPSEDDIEEEGDQEGEVDEDALVAQYEGLVALSEDEESGDERELQGGLGENVDYNEVTDLEPSEEDDDESEDIELDESADEEEEPVKKKQKKNSTESTESTNDSDAKKKQKSKKEEKEKKQKKKIALPALEYGFLDQGEFGNGXDSSDNASDLDDPFFDRPEPTKPKNRKGQRQRRLLWEKKYGKSANHVQKEIAQKREDREKRQKEFEEREEKRQQRVKEQFEKDRTKYLKKKDYESKDFHPSWEAKKVQEEKLKNVKFSGKKVVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH58</accession>
    <name>A0A1E5RH58_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Anaphase-promoting complex subunit CDC23</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1067</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH58">
        <property type="interactions" value="1068"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH58"/>
    <dbReference type="InParanoid" id="A0A1E5RH58"/>
    <dbReference type="OrthoDB" id="3972096at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007192">
        <property type="entry name" value="APC8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF10">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04049">
        <property type="entry name" value="ANAPC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cdc23" evidence="9">
        <location>
            <begin position="10"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="444"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="478"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="43"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="190"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="10">
        <location>
            <position position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="693" mass="80238" checksum="4CC94CE5D6E0DCEA" modified="2017-01-18" version="1">MMSVSMLKDLRLELRRSAMDLSKWKLMQSAKWSSEALNGLHKTSANTKIRTDKTLHYKHKQSTNHDNGEGHNGDNEKEEEEEEEEEXEEEEEEEDEEEEEAGLIMMNLHKLRIENKLSTKDADLYLYAQSLFDCKEFDRCAYVLRSSNXPCMKFLHLYSKYLSWDMKSQENMENELLSSGSSEQHETGHDTXNGSLQGTFGSSAIRKYHKLXDDAGDEXKVYRKQEQCSTASGNSGIKISIPLILNELNEYLEKFYDKFIESQPLGFSLLHYLKGILMKLQDSNRIALQSFILSLKLYPFNWGCWQEMLDCITRADEVPVLLDHLGSILDPQHIMLQFFKVAVFQQFFGNNTGEYGLNEQFLEMLDHLLTIFPDFAFLKSQQAVICYELLDYVNSEKLFDEIIERDPYRLEELDTYSNILYVMQNFSKLANLAQMCTNVDRFRPETCCVVANYYSSKQDHEKAIMYFRRALTLNKNNTSAWTLMGHEFIELKNTHAAIESYRRAVEINPKDFKAWYGLGQTYEIMGMHLYSLFYFQKSCSLRPLDKRIWNALGGCYEKLNNREKFADAINCYSRSLQLSGVDLEARYNIYSAASYQLIYKIALLYDKLNNKNMCAYYMAKCCGDLGDGVGEATGNALTEEMDKARLWMAIAEFANKNYAAAYRYASNVTTGTAKEIENARYISAESLRKSKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH66</accession>
    <name>A0A1E5RH66_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g1608</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH66">
        <property type="interactions" value="314"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH66"/>
    <dbReference type="InParanoid" id="A0A1E5RH66"/>
    <dbReference type="OrthoDB" id="504170at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000399">
        <property type="term" value="C:cellular bud neck septin structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032161">
        <property type="term" value="C:cleavage apparatus septin structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005940">
        <property type="term" value="C:septin ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044879">
        <property type="term" value="P:mitotic morphogenesis checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14081">
        <property type="entry name" value="STKc_BRSK1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000394">
        <property type="entry name" value="Serine/threonine-protein kinase HSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.220">
        <property type="entry name" value="Fungal kinase associated-1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031850">
        <property type="entry name" value="Fungal_KA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043024">
        <property type="entry name" value="KA1_sf_fungal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16797">
        <property type="entry name" value="Fungal_KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 13 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="36"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="392"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="798"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1070"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1181"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1352"/>
            <end position="1375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1390"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="408"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="429"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="467"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="505"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="536"/>
            <end position="551"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="560"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="808"/>
            <end position="820"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="845"/>
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        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="931"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="990"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="1075"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1104"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
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            <end position="1164"/>
        </location>
    </feature>
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        <location>
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            <end position="1198"/>
        </location>
    </feature>
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        <location>
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            <end position="1224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1232"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1399"/>
            <end position="1411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1412"/>
            <end position="1424"/>
        </location>
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    <feature type="active site" description="Proton acceptor" evidence="12">
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            <position position="173"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13 15">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <feature type="binding site" evidence="13">
        <location>
            <begin position="177"/>
            <end position="178"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="14">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010791"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
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            <dbReference type="ARBA" id="ARBA00012513"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022527"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022741"/>
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            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
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        </source>
    </evidence>
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    </evidence>
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        </source>
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    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50011"/>
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            <dbReference type="EMBL" id="OEJ86249.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1552" mass="173450" checksum="E8FC191539CC98BC" modified="2017-01-18" version="1">MNDVQEQHTRQNETTTNNPTTHSKHVQNTSSQIGPWKMGKTLGFGSSGKVRMAINTKTHQHAAVKIVSKSLFTKSQTNTSTIVEDQDNDFLPYGIEREIIIMKLLNHPNVLRLYDVWETDTDLYMVLEYVERGELFNLLVEQGPLGEKEAVRFFRQIIIGISYCHALGIVHRDLKPENLLLDHKLNIKIADFGMAALETKDKLLETSCGSPHYAAPEIVSGMPYHGFESDVWSCGVILYALLTGRLPFDEEDGNIRKLLLKVQKGKFSMPSNISPDARDLLSKILTVDAKQRIGTREILKHPLLKKYPNMKDSKSIKNLPREDTYLNPLANGGSENIDQSILNNLIVLWHGKKREEIVKSLKDPGANLEKTFYALLYNFKINVQLQNQLSSSQEELEEQKKQQKSQQHGQSNHTQQDMPKAKTPSPQKQQRLSVYQNQQQQQQQSAKQPQPQQSAHHTHSHRAAPGNGNSVSPPKQSGNTKKRLSTISVSSSHRRPISFIKPNGTSSSSHNLVTTSSSSNSTPSAHSLGSTPVKRTVTTSSHSASSSMDSVPPVPRNLLESYTSSTPNTSLTDNEVSTLTVDNSTSTNDTVLTQGSSFENRSSSPVPTANGVASGHAPSVPVSSIVSNPKRISRTISKRFSRSGSRASLYGGTGSNGSYGTLINPQTKLKHGSITTKLIATYAKLSELQDEEWGYIEQETRRTSQTFATLIDEVFEYEKHEQIRKEKLELQRKLKESKLREEQEKLQKQRDLLEQEKQELKIQEEQERLVKKKQISRDRLQKMEQKKRELEKMIARLREGIDGEADNSDDHESVSNKETDDLTSVETADNDRETETGPGEEEQMEKESESGFKIEDYGRQSPTEQTQPRDDAYELNGYSEGQNANTEKRFESGQENVNNYRSISEPTPAQKRLSNSDAFVKPGATGKHSALDNNILRSTQKRSFSLNTRPVSRLDPGLAAFEDHNNRNSRISAASQRKKSFRKSTISTVEESESGSDYDNDNEDDDDDEGYTTIIDEDEVERRIVESLRRSKFLGSQFDLAMKLAGGNNVSAASKRKSILKPNNHAAETIVAHGGSKKQTKSKAKNSKKTKKLESKQNKRGMLSASSSMTNLAEKSEPLENMDSEMEPSEGHTKLSEVTVPEVTRKSNDQEADKSHKSSVDKKRLSVLSLYSTKQSYRDLAAELKNDTNDTSTEKLENPKPATSDEEQFVFDDNAVQNTDSTAQPDEKKVQPSEQVNQSTVSDGMVQSRSSQVRLNFADRFKAKQSPAIKQEANNNENKTDEEVDDTPVQRKVELPTLPPLDKSSTHATGLGLVSQVDSTKPADSLVKDAESASANVTEPLVIDKNTNRTTMNSARPIENKETSASKPEPMKSIPKKKSGIFRLFSSPTKEAAKETVKNENNTKSSFYGNEQQQKNANQPAQKPSSSAIVHGDSFVKIICNAKSPTLSQLKLLNIIYEKLCQWERSKVGLKNVKMNSRQYLIRGKISNDNILSLRSTTFQILCSIDTSRSHQANGANAQNIIIVNKLSGSNKTFHKFTFEFENFLKNQDIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHJ0</accession>
    <name>A0A1E5RHJ0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Putative ATP-dependent RNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1236</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="interactions" value="971"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHJ0"/>
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    <dbReference type="OrthoDB" id="5600252at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23827">
        <property type="entry name" value="RWD_YLR419W-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056328">
        <property type="entry name" value="DSRM_DHX29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF267">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE YLR419W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24385">
        <property type="entry name" value="DSRM_DHX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBA" evidence="6">
        <location>
            <begin position="373"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="RWD" evidence="7">
        <location>
            <begin position="436"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="617"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="868"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="232"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="818"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="818"/>
            <end position="828"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1472" mass="166624" checksum="382C455601B9974D" modified="2017-01-18" version="1">MGKKSSKKANNEPVVVAVADGKKKGGKNKKQQEPTPDEKLKNNNSNTNNRSKVTQTSSWTGKLPHTLLHEQCQKRKWNKVEYDMRRIGDKGMLATAILSYTDPKTRETLTVKMNDPVYRPNGEGVVDPQETPLEARHFASTLALHRIAYNVNLQMMLPPNHRILWNKLETYRKALLKDNPAQCAKIFDTNPFKTILEERKLREQEEKQRQAKIEQDRRKKMNEPVVLMEALNLPDNPSTKGMKVSKAPHTKNQDQRSKKPKLISFPKKVWESTVFIDLSEQARLTIEQKVKKHINWIAKQTSATASEMSTNQENLKSLLLSLGFSKNHVEESLRYSDPLSFLLFNLPAEDLPAYFKKRQEDSKNVINFNTLPLADKNKIERLMEIGCSMDEAQYALEVNEWKENQACSYLTSSILASGVSSIKYEEEELDPDMWGNEVGSLQAIYGEATFKLLEPDLCLITFNSQLKMKVYKTAAYPFEIPGLIVSTFSKSIVLPNYIKREILTKLVRNIQENNLLGDMMLFYICDWLQENVSKIVENPGPLLDSKYLSSLALSFNEGVAGLQKSTKRRGNNTAKLSNEEIAKLKNQYKLKLTTSEYKQMISQREKLPAWKKQNLIVDIIMNHQVTLITGETGSGKSTQVVQFLLDELISQKNDFNTKIICTQPRRISAIGLGERVSDERCTKLGEDEVGYIIRGVNKTKPHTRIKFMTTGVLVRILQGDLSMLNDTIVVIDEVHERSIDTDLIVILLKNVMKKVKNMKIVLMSATVSVEIFQKYFKNLGQCHIEGRTFPITEYFLDDIIPMIDFKINRGFNSRYDQDDVYDDDDDDSQNGSGVSKRNNDQYVSPSLDSHFFKSGQINYDLISETVQYVDKKLLSEKNDGSIIVFLPGVAEIDKCCRNLRSLGGGEFVVLPLHSALSPDEQKKVFNKYKNKRKIVVATNIAETSITIDDCVVTIDTGRAKSMFYSAKDNTTRLVENFISKAESKQRKGRAGRVRSGYSYKLYSQDIYENKMIDLPIPEIRRVALESLYLNVKAMNINDVIGFLSTGLDPPPLTAMKKAESLLTTGGLLSEIDQSLTQLGKYISMMPVMDAKHGKILIYSILFGCADIGVLIASVLSVSDMPFIGGFENRDKIKRIVMKHGKEDGDLLAVIKLVFEYLNIPMNSARRNFLHENLLSYNKMNEIASSNSQFYSILKDTGFLPFNYKCGDKWFNRNGDNMKIVRSILTGAFYPNLARVQLPDPKFAMTSAGAVEKDPDAKKIRYWIRNEEYIEKVDDLLQXKLEPNKDISNGKKNGKNGNDLSVLPATKAFIHPASVFFDTSGNSSGSVEELSGYDNNNVVKKDGSIDKTFSSSFILFNSSSETTNKDYSKLYLRGITPTNTISVLLFGASFTFETSDSLDGHSSGIVVDKWLPVRTWSKNGVLIKELRLLLDEAIQLSLENPAYKKETNAELTSIREKTCDILEVVEQVIQTED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHJ9</accession>
    <name>A0A1E5RHJ9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lipase 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1018</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHJ9"/>
    <dbReference type="FunCoup" id="A0A1E5RHJ9">
        <property type="interactions" value="43"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHJ9"/>
    <dbReference type="InParanoid" id="A0A1E5RHJ9"/>
    <dbReference type="OrthoDB" id="10049244at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021771">
        <property type="entry name" value="Triacylglycerol_lipase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF44">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11815">
        <property type="entry name" value="DUF3336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="5">
        <location>
            <begin position="207"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="4">
        <location>
            <begin position="238"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ86371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="672" mass="77610" checksum="90C5260BC2CFB5EF" modified="2017-01-18" version="1">MPITLKQRINKFFVYLTLYTASHVPRPVQSLIFFIFNATRFLARLCLEIISPRSKKIYKEMVNSLDQVETLPEWIEGAEKVDELTGADLWRRNFVSKRYDFQTILDQYMTMCEDIGETTTVDTHGSNFVDFEHLQYMFTTASALMLRNFAGISDKRLYSKSFLGTKILIEQYINKIIYILDFFEHNSEIIKTSFYHCCKLSLGTTALILQGGSLFGLYHIGVIKALFNNGLLPRIICGSSMGACVASLCCCLSAEQLDTYLNSYDFLINKIKNDTELLKECGYGDIENEIKLTQMISNIIFRGYSKDVYLMYMFIRKHVVGDMTFEESFALSGQVLNIVLHPKEKKNACPTLLNYVTTPNVLISSAIACSLSSEVVPNCKLLCKNFKGEVEDYFIDTHNIEFLTPQQHNDSMESGERNAYTRLTELFNVNNFIISLARPYLSYLVTNDIKHDIKISKYYSYKNPAGVQKPVKKDKTLPPSIKRTTDDLVNAAFSKTESANALKATTTFSDYDYDENPLLRIKMKYHFERKLKQVITMEIKHRMDVLDDFGYLNHWIKKLTIDEKTPKTATEVTIVPQMQHISIPRIIEGRLDNIKYWIDCGERSTWSVLPLVRTRCAIEFKIDEIIQKRHFEQQQKWNYGKKQETANSKSHTRKLRCLSHLTVLLSVQVLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHQ6</accession>
    <name>A0A1E5RHQ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome b-c1 complex subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1649</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the UQCRB/QCR7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHQ6"/>
    <dbReference type="FunCoup" id="A0A1E5RHQ6">
        <property type="interactions" value="207"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHQ6"/>
    <dbReference type="InParanoid" id="A0A1E5RHQ6"/>
    <dbReference type="OrthoDB" id="425749at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1090.10:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1090.10">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003197">
        <property type="entry name" value="QCR7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036544">
        <property type="entry name" value="QCR7_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12022:SF0">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12022">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX 14 KD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02271">
        <property type="entry name" value="UCR_14kD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000022">
        <property type="entry name" value="Bc1_14K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81524">
        <property type="entry name" value="14 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="126" mass="14174" checksum="32A0B52FA757488A" modified="2017-01-18" version="1">MPQSFTSIVKMGDAILKSPSLSKIAIPVASAFGYLSGYRELGLRYNDLLSEENEIVQTALRRMPAEESYARIFRIIQAHQYELTHHLLPASKQTTAKDDVPYLIPYLLEAEAAAHEKEALNNLEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHU8</accession>
    <name>A0A1E5RHU8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MICOS complex subunit MIC10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1657</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Mitochondrion inner membrane</location>
            <topology evidence="2 9">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the MICOS complex subunit Mic10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHU8"/>
    <dbReference type="FunCoup" id="A0A1E5RHU8">
        <property type="interactions" value="91"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHU8"/>
    <dbReference type="InParanoid" id="A0A1E5RHU8"/>
    <dbReference type="OrthoDB" id="1916310at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007512">
        <property type="entry name" value="Mic10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21304">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21304:SF0">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04418">
        <property type="entry name" value="DUF543"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="86" mass="9023" checksum="A839ABE3E2559914" modified="2017-01-18" version="1">MSQKTASTNAITTPSATILDNRWDVVLSNTVVKTGMGFAAGAIASVLLFKRRAFPVWVGIGFGLGRGYSEGDAIFRSSAGLRSVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI05</accession>
    <name>A0A1E5RI05_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tyrosine specific protein phosphatases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1288</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI05"/>
    <dbReference type="InParanoid" id="A0A1E5RI05"/>
    <dbReference type="OrthoDB" id="449382at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026893">
        <property type="entry name" value="Tyr/Ser_Pase_IphP-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF1">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13350">
        <property type="entry name" value="Y_phosphatase3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ86517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="660" mass="76072" checksum="E9F028F8E5EF25FF" modified="2017-01-18" version="1">MTNTNNLDLATQPERTNESKLNGVVNHTDTIKPCLKTTNETKIAGRNSNYITITENDTLLVQKPTIPPKDSQDLIYIKHKKYHKNQGIAGILSKPDHLNDQKKAMLLLHGHTGHKNNLHFPMIDAHLHKAGYYVLRIDFRGLGDSEDSFDPELGRTIQQDLQDIETCYLFFXTILKVQLNTVMAHSRAVMSMFEFQLKVLQSEKYTDFVPNLINCSGRYDSMGLQTKIMSHYPNWIKDKGYTCTAPRYGTRSEIFIPIKESQSAINVNTASFANIDPRCFVLSIYGAKEDIMPLSAATKFAKLFKNRHRLEFVDYADHNYFGLPNDPNPLGLPLRNGKVNYNSLVADLVTNFLSPQEQLLTFNETNSLIFLKKLDLYKNRWPLPYKFSKVLNFRDCGGYLTENGTSMVKPGIFYRSANLVDLSPMGVQCMDKMLHVKQIFDLRSEHECEKWGIVDCKNYSNWXLLSFKRDLDPEEIIKNFKNMTLGGQYFPQCYLQTLYSSLPAIKKFFQYLLANXVNEFDHAVVFHCAVGKDRTGILSMILLKILGVDDYTICMDYTLTRLGLDLQVSKIDNKFIECIMSRDYEFYIHAMGQEQYDNLVENFDMTPLKMNHNIMSCEFVAMKLFLDLFESTFASVENFFTKNLEFTAEDIQKLKTKYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI09</accession>
    <name>A0A1E5RI09_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphotransferase</fullName>
            <ecNumber evidence="8">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1633</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the hexokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="8"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI09"/>
    <dbReference type="FunCoup" id="A0A1E5RI09">
        <property type="interactions" value="1119"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI09"/>
    <dbReference type="InParanoid" id="A0A1E5RI09"/>
    <dbReference type="OrthoDB" id="419537at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00180"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008865">
        <property type="term" value="F:fructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001678">
        <property type="term" value="P:intracellular glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000034">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.367.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001312">
        <property type="entry name" value="Hexokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019807">
        <property type="entry name" value="Hexokinase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022673">
        <property type="entry name" value="Hexokinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022672">
        <property type="entry name" value="Hexokinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443:SF30">
        <property type="entry name" value="GLUCOKINASE-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443">
        <property type="entry name" value="HEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00349">
        <property type="entry name" value="Hexokinase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03727">
        <property type="entry name" value="Hexokinase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00378">
        <property type="entry name" value="HEXOKINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51748">
        <property type="entry name" value="HEXOKINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 8 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hexokinase N-terminal" evidence="10">
        <location>
            <begin position="22"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase C-terminal" evidence="11">
        <location>
            <begin position="293"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="163"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="167"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ86531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="548" mass="60536" checksum="16BEA27F1F8376CC" modified="2017-01-18" version="1">MSFQQLHRSKQFELEQEVGDIVESFTIGAREVCDLTYYYIKAMENGLADDSATESSTESTTKSESEAAEEKLARSRHRLPMIPTYVFDKPDGTEQGIVLAADLGGTNFRVCSVELNGDHTFKLNQQKSKIPEDLLEDDGVTSEQLFAFLAKKISLFVNTYHGDETESSSTSSSTTTPSSTVKENVDPETQEALTSTNQKTLKLGFTFSFPVDQTALNRGTLIRWTKGFKIKDCVGKDVVELLQTELQKIKDPTTQKVKVVALSNDTVGTFLSGCYSSLNGSTSAVGDISEPVIGCIIGTGFNMAYMEEIKNIKKLGPQLLQKYEQEGKVYMGINTESGSFDNDLVKLPTTKYDTIIDTQISPNPGFHLFEKRISGMFMGELLRVILVDLNGRDLIFHDYKPGKKNLPHRLTTPFELSSELLSHIEIDDSTDLRETELNLLQSLRLPTTLHDRVMIQKLVRAISKRSAYLSIVPMAAIIIKTGALKKKYHGEVEVVCDGSVVEYYPGYKSMQRDALAMSPLGPSGERKIHLKIAKDGSGLGAGLCALVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI21</accession>
    <name>A0A1E5RI21_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cell wall synthesis protein KRE9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1298</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in cell wall beta(1-&gt;6) glucan synthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KRE9/KNH1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI21"/>
    <dbReference type="STRING" id="56408.A0A1E5RI21"/>
    <dbReference type="InParanoid" id="A0A1E5RI21"/>
    <dbReference type="OrthoDB" id="2432613at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006078">
        <property type="term" value="P:(1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042546">
        <property type="term" value="P:cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045328">
        <property type="entry name" value="Kre9/Knh1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018466">
        <property type="entry name" value="Kre9/Knh1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008659">
        <property type="entry name" value="Kre9/Knh1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28154">
        <property type="entry name" value="CELL WALL SYNTHESIS PROTEIN KNH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28154:SF1">
        <property type="entry name" value="CELL WALL SYNTHESIS PROTEIN KNH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10342">
        <property type="entry name" value="Kre9_KNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05390">
        <property type="entry name" value="Kre9_KNH1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184759" evidence="4">
        <location>
            <begin position="28"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Yeast cell wall synthesis Kre9/Knh1-like N-terminal" evidence="6">
        <location>
            <begin position="34"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Yeast cell wall synthesis Kre9/Knh1 C-terminal" evidence="5">
        <location>
            <begin position="172"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="282" mass="30858" checksum="DD904718551BE576" modified="2017-01-18" version="1">MVQLTTQSRFLISIMLGFLLCMRFVKADVSMVKPQTGATYKVSGSSVDIDVMWEDDGNSPTLDEVSTYSFTLCSGPNSNIEAIKTLATRIKASDISDYTYTLTIPETLSENGIYYVQVLAKGTFGYTIHYSGRFTLSGMSGTIVASSAGAISTPPAPQYMITQGTNAKPTINTAWFTIPYTEQTGISRFAPMQSQPKSSVDLSKYSTWTRRFPTSAVTYYSTFRKTMDCSTTITPGWSYTLTSDINYQTADAGPVSNGGWYDPKERMSLSTRKVNGKVATAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI24</accession>
    <name>A0A1E5RI24_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">General amino acid permease AGP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1614</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI24"/>
    <dbReference type="FunCoup" id="A0A1E5RI24">
        <property type="interactions" value="272"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RI24"/>
    <dbReference type="OrthoDB" id="3970526at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF17">
        <property type="entry name" value="GENERAL AMINO ACID PERMEASE AGP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="426"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="450"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="500"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="533"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="7">
        <location>
            <begin position="94"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="605" mass="67061" checksum="73E02A88F4825F8E" modified="2017-01-18" version="1">MSNIASNNTAFSDSKLESTVKIDEAGSDYIQDTTTPQKPVFNSQRLQNFVDSFKRAEQDDSDADFDKLESSPSQQSQAFGKTSQLKAKIKPRQVFLIQLGTGIGSGLLVGNGTALRQAGPAGLLIGYICVSSFISCTYNSIAELGISYSGLSGSYHEYSGILVDRGFCFATAVIYAIQWVCALPLELTTASMVIKYWTTSVNPDVFVAIFYVLLVIVNFIGGSGFAEFEFIMNSAKVCMLLGFIIFGIISDCGGSSGNDHTYIGVKNWHAPVNAFWGDSALDHFKGTCSSFVYAAFAYSGFESSVLSASEQVNPRKSLVKAGKMLIYRVVVIYCLSIFILGLLVPHNSPKLLKSSGSSATSASPMVIATMNITALPHIVNAVILMAVLSVGNNALYVSSRVFLSICEQFFPKTPYLTYVDRRGRPLFGLILAAVIGLISFVAASPKESLFFTWLLSISGLSALFTYLSMNLSHIRFRKAMKAQGKSLNEMAFLSRTGIWGSYYSVLVIIFIFCVQFWIALYPIDSNKPNAENFFQHYLTMVVFVILYLGFKIATKEWHFFIPSHKVDLARGRRIFDHEMIEQEKLEERERARNSPWYIKVRDFWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RJ09</accession>
    <name>A0A1E5RJ09_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1529</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RJ09">
        <property type="interactions" value="867"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RJ09"/>
    <dbReference type="InParanoid" id="A0A1E5RJ09"/>
    <dbReference type="OrthoDB" id="292964at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035927">
        <property type="entry name" value="DUSP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF24">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143791">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="408"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1172"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1228"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="41"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1173"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1241"/>
            <end position="1270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1313" mass="148142" checksum="1B8372EBC98F9859" modified="2017-01-18" version="1">MLVSNDTEQSNEHPEQTSYGDSSIPEQHSAAPPLPARPHAINTTMFSSQYNQSSGSAEPEFENIEVPMSDAQMTTRQTSKGESFAPYNTHQPDLVSVTADNDDDLYESDLAPLANTLDDTESTSASDPAESQEQIISKELPSLETQREIILRLYQQDLLERKAGDSIFIVPNQWFAAFWSGNEPALFEDPYAYLGSIDVEPFFVDCDNFLFKDRSVLYPVCKSIIMKLAEWYGVQNNIACTYLIENPEYDPANDVPSAKLMVDWDWLDFYLFLFFEEAVQTMNKTPIRIRLSKALTIGSILEKCKAFLLDHENLQFEKAKTRFWLHLLDETTVSSNDLQEYSDNFVLLPPMFAKMKNKSPIKPKNCSQSLEALSSTQPLFIIAEIKQKEHWPSNYYIYNKPALATGTKGLNNLGNTCYMNSALQCLAHIPEFEEYFKDQCYKSELNLENPLGYEGKVATSFGELVSNLFSPNTPSVVAYTPRYFKSCIGYCNSMFQGYQQQDSQEFITFLLDGLHEDLNRVLKKPYVEKAELEPGFDINDSQLIEKLATKAWHDHKLRNDSIILDLFVGMYKSTLTCPSCNNVSLTFDPYNDLSLPLPVQSFWTTTVLIFPLNHFPIYMEVELDKKSVYQDLKQYVAKKLNRDPATLLGYECFNNQIYKSYDAPGSDASYLPLSDLIGTQDTAVFVEVAPMEKGDLLVPVFSTFREPGAAHGKFFGYPTFINFKKEENLSLGKIKYKILKTMESLTGLEICVPFDKSKNAETYSQYQAKTKALIENEHFELKAEDLGFFDKEYDFWKLEYNNFCKIKIISAPSANSTLDDDLYPEMWSPSNTFNPNDGTDLSSLTNPVLSSLYSGDGCSMTVDKLSLDSSGENEHDTNYTGSTLEKQNSHTLADASCDIAAEPEITGDGVANSAVRTSEPLETGSEITFRPIITGNAVIVVEWDNVSEATAQNFSWNHPKEFVNKELIEIKEKRLADGQKTITLDACLEKFSKPEVLAASEAWYCPKCKKPTQAVKQIELWSTPDILLVHLKRFQNQSSFSDKISDTVEFPIDDFDISPYLAHLDPKTQHLYELFAVDNHYGGLGGGHYTAYVKNCVDNKWYYFNDSRVSEADPKDSIAGSAYLLFYRRKTSSLLGGETTRKLLEVGRKQAKDEHENFMKAQLKLYETTKSDLEEEFEESGDADGSSESEDDVASNGDEKGNSNAHNMDNEENIKNHHQVPILSERNILESSGVATDKANSEQPTNHTIGTPISLSSMDESSRNASSLELNNEVDIERRATNPLPVGNENGDTESSNNERRKIRLISKMSENE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN20</accession>
    <name>A0A1E5RN20_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Nucleolar protein 13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g656</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN20"/>
    <dbReference type="FunCoup" id="A0A1E5RN20">
        <property type="interactions" value="961"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN20"/>
    <dbReference type="InParanoid" id="A0A1E5RN20"/>
    <dbReference type="OrthoDB" id="1875751at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12397">
        <property type="entry name" value="RRM2_Nop13p_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000863">
        <property type="entry name" value="Nucleolar protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034226">
        <property type="entry name" value="Nop13/Rnp24_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF95">
        <property type="entry name" value="NUCLEOLAR PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="117"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="223"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="79"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="293"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="18"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="294"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="325"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ88288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="389" mass="44351" checksum="2621CD4CD169E0C8" modified="2017-01-18" version="1">MSEEETKIVETATTPTYDVEKALQDPSKKRKAEDEIEIDLKQSVPLSKKQKRMLRRGKVTLEEINKKFNIDPSSIKEYEQELKEKKEQQEQQEQQKEQEALDSTSAQKEKKQNVERFGVWVGNLSFDTTKEDLQRLLVLKIEELTESDILRINMPLQKNDGKQIRNKGFAYVDLKTEPLQNAVIALSESQLNGRNMLIKNSKNFTGRPDKEDLVAASKNPPSRILFVGNLSYDVTSELLEAHFQHCGPIARVRMATFEDSGKCKGFAFVDFKTEEGATNALKDKAVRKIASRPIRMEYGEDRSKRQVKRRPQESTPTNASGSFEGKFEARSKQDFKPQRSNEPPREFKPRNRDNARDKNNRVKSSIALANAPRASAAIVPSSGKKVTFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN89</accession>
    <name>A0A1E5RN89_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">homoisocitrate dehydrogenase</fullName>
            <ecNumber evidence="12">1.1.1.87</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g851</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(2R,3S)-homoisocitrate + NAD(+) = 2-oxoadipate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:11900"/>
            <dbReference type="ChEBI" id="CHEBI:15404"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57499"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.87"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-alpha-aminoadipate from 2-oxoglutarate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.87" evidence="12"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN89"/>
    <dbReference type="FunCoup" id="A0A1E5RN89">
        <property type="interactions" value="675"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN89"/>
    <dbReference type="InParanoid" id="A0A1E5RN89"/>
    <dbReference type="OrthoDB" id="10261637at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047046">
        <property type="term" value="F:homoisocitrate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009085">
        <property type="term" value="P:lysine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000012">
        <property type="entry name" value="Homoisocitrate dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF48">
        <property type="entry name" value="HOMOISOCITRATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="13">
        <location>
            <begin position="22"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ88352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="367" mass="39668" checksum="10A4454BD1BD56F3" modified="2017-01-18" version="1">MLSRSFIRQSARLYSSAPKTLTIGLIPGDGIGKEVLPAGRQVLDNLSSSHGLKFNYIDLEAGWGTFEKTGKALPDETIDILKAECNGALFGAVQSPTTKVEGYSSPIVALRKKLGLYANVRPIKSVAGTGDRPVDMVIVRENTEDLYIKEERSFVDANGKRVAEAIKRISETATEKIAKIALQIALQRYDVNGKATLTVTHKSNVLSQSDGLFREVCKKIYQSDPALYGKIGYNEQIVDSMVYRMFREPECFDVVVAPNLYGDILSDGAAALVGSLGLVPSANVGDNFVVGEPCHGSAPDIAGKGISNPIATIRSTALMLEFMGHPEAAQQIHKAVDLNAVENQIKTPDLRGKNSTQDVINDILSKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNH8</accession>
    <name>A0A1E5RNH8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein HLJ1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g945</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNH8"/>
    <dbReference type="FunCoup" id="A0A1E5RNH8">
        <property type="interactions" value="201"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNH8"/>
    <dbReference type="InParanoid" id="A0A1E5RNH8"/>
    <dbReference type="OrthoDB" id="1507364at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071218">
        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051100">
        <property type="entry name" value="DnaJ_subfamily_B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908:SF3">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="24"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ88438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="229" mass="26005" checksum="76B75FAAD39CBF88" modified="2017-01-18" version="1">MAEREYTKEQESVTLEVLKHDKHDFYKILKIEKTSNDLEIKKAYRKMAIKLHPDKNSFPRAAEAFKRVNRAFEVLSDEQKRKIFDQLGVDPDDRHAASAAGRGNPGSAFRSTNDPFRQQGSPFGGPGGMGFASPEDFFQHFAFGGPFGGGPFGGGGNGFQTFTFDPSTRTFHSRSSGAQRRRNPDGTTTTAGERQRQNRENQEEDFMPWRIIMPFLLLFLFQVFEKLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNI7</accession>
    <name>A0A1E5RNI7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA damage response protein kinase DUN1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g943</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHEK2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNI7"/>
    <dbReference type="FunCoup" id="A0A1E5RNI7">
        <property type="interactions" value="509"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNI7"/>
    <dbReference type="InParanoid" id="A0A1E5RNI7"/>
    <dbReference type="OrthoDB" id="407410at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000732">
        <property type="entry name" value="Protein serine-threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FHA" evidence="5">
        <location>
            <begin position="62"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="210"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="522" mass="59138" checksum="6E5C1918DA74A145" modified="2017-01-18" version="1">MSLKRKYDVFNELEENTSLEQAQNGSKLEGFHLAENEVVGRLFNMSSEKEHNVLEIRYKSVFIIGRSRSCDLTLNGADISTKHCAFYFVENKAEPETPHLINIIDTSRNGTFVNGNRLVRKDCILKNGDTITFGKTSSFLFRYSNASFTANSQHDIRACKDEPLKGGTGHEAEIVNDVFKRPSQSTQPGVRKALSVKNIVQRPTSVFDNYILGKELGVGHYATVKEGKNKATGQTVAIKIFHAQKTNDRKKMEQFKTETNILIKIKHENIVRLLDRFIEPVSKSSIQTYLVLEKVSDGELFDRIIKKGKLPQEETKAIFRQILSGLKYLHNNGIIHRDIKPENVLLSIKKRTSKDQTQDGPWDENELSICVKIADFGLAKFIGEMQFTNTLCGTPSYVAPEVLAKKTYSNKVDIWSAGVLLYVCLCGFPPFSEQLGPPSMKEQIMEGKYEFYSPYWDDIDDIVLDLIANLLVVEPDQRFSVEDTVNHPWFANIIPQSQLRVIGRPPLSQNQTKPSSISEIMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNN4</accession>
    <name>A0A1E5RNN4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g963</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNN4"/>
    <dbReference type="InParanoid" id="A0A1E5RNN4"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ88474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="380" mass="45006" checksum="F5FD76660B2861AF" modified="2017-01-18" version="1">MNHQAENDEFDVTCDTDSEEFTNFTGASCNFAIDDIIVIEYLLAQCFCTTLCDMLQALNFDSRMIEKFFQVYKMWERQHVINTLMSTDFDYYEFSEYICSHFDRGFQFLNGNLPQMIDYDEDFVDFRFKLYTLHVISMIQRNEMAHEIFEFMKENLMPYKHKENIYYRKVIDKLAMFTLMGVKKEVSADAEGQEKDGQNNDKDTLSFERFQSQLYRASLANKMNYLLLDLEFSAIHFFQSTCKLPEFIKKHEIYKGTPLFDAEKYIERMKEMYKGTNAQNGHDHYGDKRYEFPLNSDGGTKVDIIGLINVSQMLQSAPLTFTSATWNYNELKNVEMRKSQQEDMLLSQDNTFYHVYKPRIAELFSANELETHLAERYAME</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNU9</accession>
    <name>A0A1E5RNU9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein IBD2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g668</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNU9"/>
    <dbReference type="FunCoup" id="A0A1E5RNU9">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RNU9"/>
    <dbReference type="OrthoDB" id="4057723at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="423" mass="46827" checksum="33CE32CEE3E6E451" modified="2017-01-18" version="1">MSRRPKNASIEVVSKNGPVNVDTMIKEGVNAMLRTFNNNELWQSEQQNNVIVEQQDSEPVHNAKEAETADVMQKFTQQLFSTVSQLSGTDGSATTNPNTFQIPETALNSKMGQDILQAMCHLIPGGPPNAKKFMEDFAQQQSSQQQSPQSGFQNLPQILDNGGIAEAEFIIDYNDGNMDMEKLNETIYEKFTDILNGSANGQMPVLGWEGNQQGSQEAKPSSKKKKSKKKKKKNKNKNKNADNKEGEIVNNKDEPLTHPETVSALNNDTSGKNADQGFTDDAHIQHNRPLPSSCGPACLHDKPAHPNIETKTTINKTDPIDELAALTEAKLRSLLPAEALGNSHSLAKIQEDNTERAPNFSYLAYVDEPLCYFCEYYLVFGELPVNIIKSYYRKASSSSAKQKPSCCGCHTEAGPHRHHHEHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNZ0</accession>
    <name>A0A1E5RNZ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">THO complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g751</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the THOC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RNZ0">
        <property type="interactions" value="648"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNZ0"/>
    <dbReference type="InParanoid" id="A0A1E5RNZ0"/>
    <dbReference type="OrthoDB" id="3972783at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040007">
        <property type="entry name" value="Tho2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021418">
        <property type="entry name" value="THO_THOC2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021726">
        <property type="entry name" value="THO_THOC2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032302">
        <property type="entry name" value="THOC2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21597:SF0">
        <property type="entry name" value="THO COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21597">
        <property type="entry name" value="THO2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11262">
        <property type="entry name" value="Tho2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11732">
        <property type="entry name" value="Thoc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16134">
        <property type="entry name" value="THOC2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="THO complex subunit 2 N-terminal" evidence="9">
        <location>
            <begin position="69"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="domain" description="THO complex subunitTHOC2 N-terminal" evidence="8">
        <location>
            <begin position="712"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="domain" description="THO complex subunitTHOC2 C-terminal" evidence="7">
        <location>
            <begin position="1017"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="domain" description="THO complex subunitTHOC2 C-terminal" evidence="7">
        <location>
            <begin position="1152"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1510"/>
            <end position="1660"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1282"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1510"/>
            <end position="1520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1538"/>
            <end position="1586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1589"/>
            <end position="1636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1651"/>
            <end position="1660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF11732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ88601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1660" mass="190053" checksum="851BAE593DDD981F" modified="2017-01-18" version="1">MIPEINALLKDPTIDWSDENSTLSNETTIPIFNADDVLQNAQLFSANNSEYIQKKLIEPLTQMLKTDTDDNNELIESLCYLFQFMFAKLQDASMSENKNDDPTLDIHPVKSGIYKLFQELSSAQSNFGRFSKSVLVSYCTSVSVNEAVLADLWSILPITIEDRMFHIKRLMSKDNTVISKYRKIHKTLCTEDTRYDTLLESTGWSELLNVILAGAFDDQVMFSDNSGEKLQKIYFSSVENIIDRFQLKISSVINIIADLMIISLEVADTEYSSTTLFSENSWKFCILMLQHLQAVCGIKPADINHLDQEGGCMELAIFLVEKINILSNSAHDSSSEKAVSCCLQAVCFLLKAGLVSFQSIYSGLSPDDATMHQYFEFYLKELKQMEIDSEKTSSALATAAALSNEDDTGSQLISKDAASFGLKSGDVAPASNTKSPDEIDAKLAELEKKRAKNDIIGKTNNCLKLLFLKKLLENGCYTPSIYLLKKYKFAHFQDPSLQTLCFRMFASMMSPISVHPDIKQFSLEERSLPRVDYAEIVMLHSAQQTLLKKSSDPLDISKTPYKVVDLFSEWSDQLPKIRDTEELIKYSHEFLFLLSPRLDYDGGEAIIQTIVNYALKRLSETPTASEEWFKFFRKFLFPSMIFNNVSVNNLLYKVMSFFPISERYLLYSELTEKLIDVNIHMKICYSMSEKDMKNFLKMVSYDKESSQCRRFANYANTNPVAVFDFVLTTIENFDKVSSILVSAAKFFSPYALDVLQFSLLKRLSKSRETVSPDGINMAPWFQNLTNFIAEIIKTQPRFHCYLLLEYIINNMDKGEYSATFMLKTIVEKVSGIQVLEEVQEQILILLNSDECLQKMARANIMDTRENNKAIANSLLQNFLQCSSNAFSVIMVYLQQYLRSVFDDRVDEKITSNRYDDAHSILWTFTEFCKYFMSADEFASTIFSFSSMIDKHDMAIPWAFHIWRAVLTRSNSFEDSMAIAKQSSYLAKSFGNDFDFFSSFWLGGLKTMLFDQSVYQFSEKYKNKVTNEHLESAKSNHMQVRLEFVTFTKNLKQQWTTELTSREAYTLLTKCILPRAQFGLEDAFFANEFLFCFFDNNEICVIMDVLFESLFASELVSLTSASVAYFGFFMKKCFEKLLDIKETLPKENILCQKLLLWSCSLTSNVAHMLTSDVYMFTRNCIQFMSKVVFTFPVLQVHVSLLIEAVEERLQTEQRNDIITPCKTLLGFLKTQYGKSVSAFDFCTISENESKEYRFFETASQALRASQLYKQELAGFRKEDEMELIRRKKEEENALALKKRLEEEAVAKKKKETELAAKKAEEELIAKKKSEDEELEKNKAKEEEATRVALRREAQLKEIQERKEHLEKSRKRQLNEDLPVASKKLKTEISPQDVAKYRNLASDVIGILRRDALQGEGVSKVIASLESYDMYVKIRSIVENPPRNYVEQFINALSFALKSYFINKIPDTFREFSNKLKFVLESNSKNLDSASAVRLLSSLKQIEHYMSKFKNSNVSASSNPSTGRVKVSETTARAPVGSRFNATKPESTATTNSVNSRNPVPTNTRSSDLQSRPSQNHAATQRQPVSTTSKSSRDRNRSSDPHRSSRRDFPKPSREGDRTKLSDSREGRERRRENESRAGRQSSSYDSGKESRFSNSYRGSRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP16</accession>
    <name>A0A1E5RP16_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-chaperonin molecular chaperone ATPase</fullName>
            <ecNumber evidence="1">3.6.4.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g610</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.10"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.10" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP16"/>
    <dbReference type="FunCoup" id="A0A1E5RP16">
        <property type="interactions" value="1838"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP16"/>
    <dbReference type="InParanoid" id="A0A1E5RP16"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000172">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000026">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000004">
        <property type="entry name" value="Heat shock protein SSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000005">
        <property type="entry name" value="Heat shock protein ssb1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0346">Stress response</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="614" mass="66460" checksum="D3E09F880EDF7443" modified="2017-01-18" version="1">MSDGVFQGAIGIDLGTTYSCVATYESNVEIIANEQGNRVTPSYVAFTPEERLIGDAAKNQAALNPKNTVFDAKRLIGRRFDEESVQSDIKTWPFTVIDQQGSPLIQVEYLGETKTFSPQEISSMVLTKMKEIAEAKIGQTVEKAVITVPAYFNDAQRQATKDAGAIAGLNVLRIINEPTAAAIAYGLGAGKSEKKERHVLIFDLGGGTFDVSLLHIAGGVYTVKSTSGNTHLGGQDFDNNLLDHFQKEFQKKTGLDISNDPRALRRLRTACERAKRTLSSVTQTTIEVDSLFDGEDFETSITRARFEDINASQFKSTLEPVEQVLKDAKIAKSQIDEVVLVGGSTRIPKVQKLLSDFFEGKQLEKSINPDEAVAFGAAVQGAILTGQSTSDETKDLLLLDVAPLSLGVGMVGNVMGVVVPRNTTVPTIKRRTFTTCADNQTSVTFPVYQGERTNCAENTLLGEFDLKGIPPMSAGEPVLEAIFEVDANGILKVTAVEKSTGKTANITISNAIGRLSSEDIEKMLHDAEEFKAADEAFAKRHEAKQRLESYISTVETVVSDPVSSAKLKKGARSKVESALSDALAALQIEDASTDELRKAEIALKRAYSKATSTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP25</accession>
    <name>A0A1E5RP25_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Tyrosine-protein phosphatase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g609</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP25"/>
    <dbReference type="FunCoup" id="A0A1E5RP25">
        <property type="interactions" value="921"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP25"/>
    <dbReference type="InParanoid" id="A0A1E5RP25"/>
    <dbReference type="OrthoDB" id="10253954at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18533">
        <property type="entry name" value="PTP_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016277">
        <property type="entry name" value="Ptp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF449">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000938">
        <property type="entry name" value="PTPN1_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="2">
        <location>
            <begin position="36"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="3">
        <location>
            <begin position="259"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ88641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="377" mass="43731" checksum="CDE0E6ABCD4B3949" modified="2017-01-18" version="1">MSNTDATMIDSNPEQQKATKPICKTPWYLDQPLGDLKMKYQYIQYKEDLRLRDATLNPETSKYSLGVSVLQENSKRNRYCDIMPYERNRVTLETLDSTNDYINASYIKLDFLANTKKYYIATQGPTRRTVNQFWTMVFQKCVLGPDVVIVMVTPLVEQNREKCFLYWPSSTSISKNMHIPRVQNVNGKNDVSIFEEDLNLEFISEEKYYHSPLSDATEHHHNHIYTKLKLTNRQTGECKNIHHLYFDRWADMSSPTSVDNILGLITHCNELNAKFGNPIISHCSAGVGRTGTFIALDYLYTSFLQNNKNLVPFVKPGSLSTQGHAFVKTDHLQQQQQLEDLVQKTVLKLRLQRMHMVQTFDQYVFLYKAVETFFAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP31</accession>
    <name>A0A1E5RP31_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytoplasmic tRNA 2-thiolation protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">NCS2</name>
        <name evidence="3" type="synonym">CTU2</name>
        <name evidence="5" type="ORF">AWRI3579_g722</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CTU2/NCS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP31"/>
    <dbReference type="FunCoup" id="A0A1E5RP31">
        <property type="interactions" value="196"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP31"/>
    <dbReference type="InParanoid" id="A0A1E5RP31"/>
    <dbReference type="OrthoDB" id="25129at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03054">
        <property type="entry name" value="CTU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019407">
        <property type="entry name" value="CTU2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20882">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20882:SF14">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10288">
        <property type="entry name" value="CTU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ88628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="509" mass="58307" checksum="4F2BCC980A73BCFF" modified="2017-01-18" version="1">MKPAIPQAQTSSNKPNKPLKNEIPKMTAVLCKRCNVSEAVVKSRKEIFCKECFTKFITLKLRKMLMSKETESVFKVIYDPKNPQREKEFNPPNCKVLLPCSFGSSSLTVLHILVDYLKEQHSQHKKYGFKLEVLTLYCDEEQLIKYKAIIEKLKSMHDFQISSDFLKFTLLDVNRYFLDNNNTAVLTIGEDYATQESVSPETRNSMSLTESLQNAFPTNKLVTSKQDLLDIFTQNIIKVYYNSDPKFKQLLWGSSMTKIADQVISNVVKGRGASIAKELEFGMYPLTDVFLSEIDAYFLLHFRDTYEELLYHYVPQPTLFLNKDTEYCDIPCNEVLAKNAKTINQFAREYFDSIEGDYSNVISTVVRTALKLDTPGDLKKIDLEDNQQNQDSNVTLCRVCESPCYQDIKDTEKKSATAWLNNITVHEGYPVETDVEKGYYHQWQTSTETSSANLHKSNLDSKTVSLCYGCSVTLHTSSSKSIAWVQKKDTDETELQSVLNDYILSDNED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV08</accession>
    <name>A0A1E5RV08_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sulfate permease 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g381</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RV08"/>
    <dbReference type="FunCoup" id="A0A1E5RV08">
        <property type="interactions" value="509"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV08"/>
    <dbReference type="InParanoid" id="A0A1E5RV08"/>
    <dbReference type="OrthoDB" id="288203at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008271">
        <property type="term" value="F:secondary active sulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018045">
        <property type="entry name" value="S04_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00815">
        <property type="entry name" value="sulP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01130">
        <property type="entry name" value="SLC26A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="161"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="298"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="340"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="465"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="491"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="520"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="546"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="6">
        <location>
            <begin position="668"/>
            <end position="804"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ90761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="858" mass="95674" checksum="71FB28737B9D7419" modified="2017-01-18" version="1">MPHELQNLEQEYYENYQSQDQHHNDQASPLQEENHPIHGGVNEYFHLDNNFEEQSFDKNDAEKIKPVTSGQYLHTNSEIKVPYYEEDVVGFKEYYNQKFRGYLGIKAAGQYLISFFPIIKWLPHYNLNWAFNDIVAGITVGCVLVPQSMSYAKIASLSPEYGLYSSFVGAFIYCFFATSKDVCIGPVAVMSLQTAKNIADVLAKLPADTKITGPEIATMVSLFCGIISIGIGILRLGFLVEFISTTAVSGFMTGSAFNILWGQVPALMGYNKLVNTRGKTYKVVIDSLKHLPDTNINAVFGLIPLFLLFATKYFCVNLGPKLINKNKRLSKKQKYYANKALFYASTMRNAVVIIVFTLISWGVWRHDKKTAISRLGTVPAGFKHVGVMKYPKGITSIVAKELPASVIVLVLEHISISKAFGRVNGYRVVPDQELVAIGATNLIGTFFNAYPATGSFSRSALKAKCNVSTPLSGIFTGACVILALYCFAKAFYFIPSATLSAVIIHAVADLMASYKTTWNFWLTNPLDLVGFLVTVLITIFDSIDHGIYFAMCWSAAVLFFKVAFPSGQFLGRVRIAEVRNPNVDFDKTRVTDTDSTSSFGAGNISGSSDKQKDFDLKVYTKNVGEAGKSSYASNSFSNKLSFYTKWIPYDYSYSRELNKDVDVLPPPPGVIVFRPSESWSYLNCSKQYDKIYNYAVATTKKGQFASEKVWNDPGEWSPPSFLKNIWKSKNDGRDAVNSLYNDERPTLKVVAFDFSSVTQIDTTGAQTLVDLRAALSKYANRQVEFHFSGIISPWIKRSLINSGFGTVNDEFANESLIIGHTSYHVVKGGEDDFEYGTATGVNLPFFHIDMPDFQEWDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV41</accession>
    <name>A0A1E5RV41_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ATP-dependent RNA helicase ROK1</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">ATP-dependent RNA helicase rok1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AWRI3579_g500</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family. DDX52/ROK1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RV41"/>
    <dbReference type="FunCoup" id="A0A1E5RV41">
        <property type="interactions" value="1083"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV41"/>
    <dbReference type="InParanoid" id="A0A1E5RV41"/>
    <dbReference type="OrthoDB" id="360161at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17957">
        <property type="entry name" value="DEADc_DDX52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000759">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044764">
        <property type="entry name" value="DDX52/Rok1_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF15">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15 20" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="19">
        <location>
            <begin position="132"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="163"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="382"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="15"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="60"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="578"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="14">
        <location>
            <begin position="132"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="15"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="62"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="584"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OEJ90817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="598" mass="67345" checksum="4E07FC070D4F98C3" modified="2017-01-18" version="1">MDIFKVLTRGANIKNTSNTSKKTTTQLQKQTDNVLTNADSQNKDLSKDLDFFHNKKIVSKVESSKREQKKEHENSFSNGKNTVESDNVQGQEDTTEVPPTILSKEDAVKLRKTYKGNVTGSDSTLPIGSFEDLISRFQFNGKLLDNLITNGFTEPTPIQCEAIPATLLNKDILACAPTGSGKTLAFLIPLLQQVIDASRTTTHNEKADKGQKNVTNGLKALIISPTKDLANQIYEECIKLSSRIVLNHNSDNVEQQRHLQVSILSKSLASKLKQSGSSNNKNRKPIDILITTPLRLIDMIKEENALDLSEVKHLIFDEADKLFDKTFLEQTDHILAHCTSSFLQKSMFSATIPSNVEEIASTVMSSDMCRIIIGHKEAANSNIEQKIIYCGNEEGKLIAIRQLVQEGEFKPPVIIFLESITRAKALYHELLYDKLNVDVIHAERTQLQRNKIIENFKNGSLWCLICTDVLARGVDFKGVNLVINYDVPNTAQSYVHRIGRTGRNGRQGKSITLYTKQDALAIKPIVNVMKQSGCAVSEWMEKMDKLTKREKQLIKSHKNKQNQDIKRKQISTVPRVIKKKKRQRVDMIEASKKRKTMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZT6</accession>
    <name>A0A1E5RZT6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Eukaryotic translation initiation factor 3 subunit B</fullName>
            <shortName evidence="6">eIF3b</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Eukaryotic translation initiation factor 3 90 kDa subunit homolog</fullName>
            <shortName evidence="6">eIF3 p90</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Translation initiation factor eIF3, p90 subunit homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PRT1</name>
        <name evidence="10" type="ORF">AWRI3579_g227</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="6">RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the eIF-3 subunit B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZT6"/>
    <dbReference type="FunCoup" id="A0A1E5RZT6">
        <property type="interactions" value="1378"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZT6"/>
    <dbReference type="InParanoid" id="A0A1E5RZT6"/>
    <dbReference type="OrthoDB" id="10250414at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12278">
        <property type="entry name" value="RRM_eIF3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03001">
        <property type="entry name" value="eIF3b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011400">
        <property type="entry name" value="EIF3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034363">
        <property type="entry name" value="eIF3B_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013979">
        <property type="entry name" value="TIF_beta_prop-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14068">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 EIF3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14068:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08662">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036424">
        <property type="entry name" value="eIF3b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6 7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 6 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="55"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="591"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="592"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR036424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ92394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="785" mass="89807" checksum="037B010ECEE42CB3" modified="2017-01-18" version="1">MTAAVEIENLSTLTEFDNIRLDDMQIDAEELAAFEKQFEQEYKISEDGNLLDIDNFVVVDGAPVAPESKIPALTRVLTKLFSQAGEIANFELPVDEETKTTKGYLFVEYKDSLSSKKAIKLLNGKKLDAKHKLLVNSFRDMDTIASDDYNVEYQEPVLEKPVSYSELQNWILDGKDQFVMSKDQMSLLAWYKGAQKPLPVVEPRVNWSDAGNLKFSPKGTFMISMHEQGVKTWGGDELVELNKFAHFDVKAVQVSKTEKYLVTFSPTPINDDVFFPKESQNHNIAIWDIQTGVLLKTFPLPPQDLVPFHWPMIKWSFDDKYCGRLGPNAIAIYEVENGFKLLDDKIMKIENVRDFQFAPAGIALGRNPQDLSSVLCYWTPEMQNQSCKATIMEVPRKRVLRTINLVQVTDVQFHWQDQAEYLCVQVDRHTKSKKSNFTNLEIVSFTGKEYPVEKIELKERVLKFKWEPKSNRFVTICIDEANLDPNVAVPQNIVQFFATTVGSDDKAPSWTVVYEITGKHSNTISWSPIGRVVVVGTLVSRFVKNSEFLFFDMDYAGEHPLNSAQWTADYNKRKQAAADAAALAAQISLGINPNQNNRNRNQPATNFNKKKATIGSTGPIKVNAAIKEIGMSKFSSVTNVQWDSCGRFFAIWSSADKHKTENGYKIYTYGGQLHREEATVGFRHFTWRPRPNHVLTNSEIKKIRKNLKEYAAKYEEQDMMESNEELRLAILDRKKKLKDWIEYRKEAAESVKPYEIFDTFGYVSTFESIEVVKEDILEEKKEKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0U9</accession>
    <name>A0A1E5R0U9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Actin-related protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g4522</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the actin family. ARP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0U9"/>
    <dbReference type="FunCoup" id="A0A1E5R0U9">
        <property type="interactions" value="1094"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0U9"/>
    <dbReference type="InParanoid" id="A0A1E5R0U9"/>
    <dbReference type="OrthoDB" id="5132116at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10220">
        <property type="entry name" value="ASKHA_NBD_Arp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000148">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000005">
        <property type="entry name" value="Actin-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="394" mass="44460" checksum="A3E1F673CD844AFC" modified="2017-01-18" version="1">MTTPIVLDQGTGFVKIGRSGENFPDYTFPSIVGRPILRAEDRNGLLGGASSIPLKDVMIGDQAQDVRSFLQISYPMENGIIKNWADMELLWDYAFYEKMKLNIDQQSKVLLTEPPMNPVKNRETMCEVMFEKYNFGGVYVAIQAVLALYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLTRRLDVAGRDVTRHMIDLLSRRGYAFNRTADFETVRQIKEKVCYVSYDLDLDTKLARETTTLVENYELPDGRIIKVGSERFEAPECLFQPSLVDVEQVGMGELLFQTVQAADVDVRSSLYKAIVLSGGSSMYPGLPSRLEKELKQLWYTRVLKNDPSRLDKFKVRIEDPPRRKHMVFIGGAVLANIMADKDHMWLSKQEWEEGGAQSLVKFGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R147</accession>
    <name>A0A1E5R147_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transcription initiation factor IIE subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3985</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R147"/>
    <dbReference type="FunCoup" id="A0A1E5R147">
        <property type="interactions" value="500"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R147"/>
    <dbReference type="InParanoid" id="A0A1E5R147"/>
    <dbReference type="OrthoDB" id="5323195at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005673">
        <property type="term" value="C:transcription factor TFIIE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001097">
        <property type="term" value="F:TFIIH-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054600">
        <property type="entry name" value="TFA2_E-tether"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040501">
        <property type="entry name" value="TFA2_Winged_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016656">
        <property type="entry name" value="TFIIE-bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003166">
        <property type="entry name" value="TFIIE_bsu_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12716:SF8">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12716">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22254">
        <property type="entry name" value="TFA2_E-tether"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18121">
        <property type="entry name" value="TFA2_Winged_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02186">
        <property type="entry name" value="TFIIE_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51351">
        <property type="entry name" value="TFIIE_BETA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TFIIE beta" evidence="8">
        <location>
            <begin position="119"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="360" mass="40182" checksum="10C99472C39B2B33" modified="2017-01-18" version="1">MSSLLDNLNKIKNKLEVPSTSTEPSKDNTPLDSTQAGISIKRERSDVSNQNDGDAEQNGEPVSKKMKSSLSANGDFSDDDGEDDDEASQSRHFGDAISNGNKNNNGSSSGSLASSLNSSQNGPGLSGTHLSTKLLLATEYIQDRGGPVSIAQIESYLSLKPESPEANNVIQLLKNINKIKFDPEEKTLQYVSIYDIHTAQDLIELLRKQPVFKGISVKELKDGWPKCNEVLDDLEKRGRVLVLRTKKDGTARYVWYNSYKMPEKIRGNIVKNLDENNPRPYQQFIAEDFRNMWEDVKLPSRADIPRKLTDLGLKPASVDPASVIKKGTITRVEVKRRKQRKSKITNTHMTGMLKDYSDRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R184</accession>
    <name>A0A1E5R184_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">V-type proton ATPase subunit G</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3959</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the V-ATPase G subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R184"/>
    <dbReference type="FunCoup" id="A0A1E5R184">
        <property type="interactions" value="288"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R184"/>
    <dbReference type="InParanoid" id="A0A1E5R184"/>
    <dbReference type="OrthoDB" id="250802at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.2950:FF:000001">
        <property type="entry name" value="V-type proton ATPase subunit G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.620:FF:000004">
        <property type="entry name" value="V-type proton ATPase subunit G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005124">
        <property type="entry name" value="V-ATPase_G"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01147">
        <property type="entry name" value="V_ATP_synt_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12713:SF11">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12713">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03179">
        <property type="entry name" value="V-ATPase_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="114" mass="12561" checksum="42FB3DFEF2083284" modified="2017-01-18" version="1">MAQTSGIATLLKAEKEAQTIVAQARQYRQEKLKQAKKDAQAEIQKYKAQKETELTEYEKKTSGSAGDLEKSAENDIQGELKEIEKLGNAKKSSVIDLLVKSVTTPTAEIHVNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1J9</accession>
    <name>A0A1E5R1J9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61</fullName>
            <ecNumber evidence="2 9">2.1.1.220</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4423</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalytic subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>adenosine(58) in tRNA + S-adenosyl-L-methionine = N(1)-methyladenosine(58) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43152"/>
            <dbReference type="Rhea" id="RHEA-COMP:10365"/>
            <dbReference type="Rhea" id="RHEA-COMP:10366"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74491"/>
            <dbReference type="EC" id="2.1.1.220"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM61 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.220" evidence="2 9"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1J9"/>
    <dbReference type="FunCoup" id="A0A1E5R1J9">
        <property type="interactions" value="615"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1J9"/>
    <dbReference type="InParanoid" id="A0A1E5R1J9"/>
    <dbReference type="OrthoDB" id="1925287at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160107">
        <property type="term" value="F:tRNA (adenine(58)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.20:FF:000002">
        <property type="entry name" value="tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049470">
        <property type="entry name" value="TRM61_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014816">
        <property type="entry name" value="tRNA_MeTrfase_Gcd14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133:SF2">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE CATALYTIC SUBUNIT TRMT61A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08704">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017269">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51620">
        <property type="entry name" value="SAM_TRM61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 9 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61 C-terminal" evidence="12">
        <location>
            <begin position="84"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="305"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="308"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="134"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR017269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR017269-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ80443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="410" mass="46523" checksum="53A4E48BD2841CC8" modified="2017-01-18" version="1">MTTEALHSASTDLASKSTFQSYKDIVQENDLVLIWISRDHIKPVVMKHGENFNTRYGTFPHTSIIGKPYGSQIATRTAKANKFGFIHVLQPTAELWTLSLPHRTQIVYTYDSSYIMNRLRLGPNSTIIEAGTGSGSLSHQLARTCGKVLTYEFHEVRCEQAKQEFKEHGLLEEDNGNLVITHRDVCNEGFKLNDRDCEASAVFLDLPAPWDAIPHLNTQGILKKNEKVSVCCFSPCFEQVDKTLKALKENGWQEIEMVEIQGKNFESRRQMVRTLDDAINILKDQKERKDAGIEKRNKMNKKILTEAAKNSKTENQTENSATTSKDETKAEVEGSGTEADKQTDATEQSPQLKKPKFNPFGKGFKVKEGDLNYNWIQVSKMESEIKSHTSYLTFASKIYDMKRETEPNDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1K5</accession>
    <name>A0A1E5R1K5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Mitochondrial distribution and morphology protein 34</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g4336</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1K5">
        <property type="interactions" value="70"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1K5"/>
    <dbReference type="InParanoid" id="A0A1E5R1K5"/>
    <dbReference type="OrthoDB" id="3972531at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027536">
        <property type="entry name" value="MDM34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058825">
        <property type="entry name" value="MDM34_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28185">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28185:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26545">
        <property type="entry name" value="Mdm34_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="DM34" evidence="5">
        <location>
            <begin position="86"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="27"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="377"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="546"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="35"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="565"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="6">
        <location>
            <position position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF26545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ80762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="759" mass="84383" checksum="0395AD89461994CB" modified="2017-01-18" version="1">MSFKFDHKAFNNKEFNELIKKKLNKHLNKPSTDENSQSNNNNNSANNNNNNNNNNNNNNNNTTTTNNNTNDSSSSFSPHTASKSKSLLTNGITVNKVNFKKAPFVEILDLNIQPTNLMVKSIIKILLESATIEISTNIEANSLLLYTSSSTPAFLMPEIICNDSFVVPITMLFSNIHLEAISNICMEKKLNSGLSVSFNDVNLDFDFDCSIKLFQGAIEKRLKLAMDSVFKDVLPVIIYSTSGAMVETKNSNKNNSINSVQAQEALSENYNYENGQHNNDNNENGQYNDDGVHDDDYRMGHYGEKTKDLTNGVTYLDIADLEDVSPVTMTNLGTMVNQRNSLSLIGSHMEGCLERPHLYKLLQKKSLQLSNLIHSATTEASASPIPSTTTTSKTSASTITSTTSTLAAKNNNKNDVNVINNTLPIENLDFNKEDDLVGKIANFQASIYQRQGRRHKNHVKKRCFKLSKKKSEEKVAEMNEMDTTRKERDAFIMXDAVAAKDVEDXXLKXNFTKKELHEKTITAEKEYXDLKPKRDTNTLRKQVLLSSTHTQDKEXDEANIPLSPPSVSGSPSPLSSPNLLAENPLASVFFHSRVATPLPSPSPVPALNFHHWVEDHASGNTVFNLNNKHEPMSQEESKQNCTTTSTHPLSNAFFPHAGSLASSPVRPVYRRNHYSFVGISSQNDHPYINAHFPVXRSSSLGDRTASNNENDTLLVSSKTCGNSPHXQDFAHEPFENMNSKAWNMSAQNGIVPPPPYSQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1T7</accession>
    <name>A0A1E5R1T7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Importin subunit beta-5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g4368</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1T7">
        <property type="interactions" value="1135"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1T7"/>
    <dbReference type="InParanoid" id="A0A1E5R1T7"/>
    <dbReference type="OrthoDB" id="3972373at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF9">
        <property type="entry name" value="IMPORTIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="5">
        <location>
            <begin position="24"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="929"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="929"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="940"/>
            <end position="949"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ80866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1043" mass="116421" checksum="C6A236A2D50795C0" modified="2017-01-18" version="1">MSVTQCIQSIEQAQSPNNSVREKAEKDLLATCDGNPAAFFQLLIDTAAASGAATTSVSLASRQFCLLLLRKMVTLYWSAGFESYKGPPGVGDEVKTHVRATLLDLALXSDQDNKIRSSASYCVVQISAVDFPDEWPALLTTIYTKIINEMSLPGLKLLIEIFDDVVTEEMFFSAGVGVETFKIIFEFLQNGRNDEVALNAKIAVLKLYGLCISQLSSSFADDFQDVVVSQLKEMNKLLCNLLADNQIFNCDNFQILSLRKQIYKDFEVLKSTNLWKKSLTTDQKKFLEQACLSDLLEMTNLKLANPGLFEEETGTNSEIKEFVIGIITFLSSIHSSFQSQEVLTNVTDFFIVLVALSESQMESWSSDFNDFVLIEAELSGTLSIRDAIYDFISEMSLSANNSITLLVTEKLTSGQFNNDFVKLEASLFLFGCCNSVVVSDRDEDTGSLQANSTEIEYLAISFLESILANSHSINEFVLARCILVAPKFLERCSDVLPNIKSCVQNVLLQTFRCLENSDSEIAKVSALIALTFYDSFVSFDTVLSKDTFLAIETMVHSIAQNLLEEAEEDTVGVIIEAMSIAVKFTPLNALKFSEFETIIHAAVKYPDNIQVVMLSKEALEGILKTCDAATYLRFADLCIPNFVKIISSSGTEYSSVLVLVLELCTAFMKMKPQEGHLPSKVVNLVLRPLIELICRSTDEGVLQMGSEALTYCIVNTELELLKPELPSLVSVLQALFNATEAASLNMGLLIVGIFEKFQSQVQDIFPQLFEAVVKKLVSANTVYLTESLMGVLCYLCYVDCTQFLEYIAQLKINNAKEGSCIEQVLSIWTTDFESIRGNLKINENIVSFSNIFFNNHVDLSNVLVDGDIIPYEGDLIITRSMAKNMPARFTKVSVYEKIVNLFADELNVVMRSSKTQEFMNIKQALEXAKSEQQSEKDLTEGVDGDDEGWEDVDDVLEYDKLNEYLSYEQDEHSHDEEDADEESFEDVDQADYGYRSALAQQKTTKELLLQFFKKAASENTHGFSNIYARLSEKNKKAITDVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1U7</accession>
    <name>A0A1E5R1U7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AP complex subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3933</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1U7">
        <property type="interactions" value="116"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1U7"/>
    <dbReference type="InParanoid" id="A0A1E5R1U7"/>
    <dbReference type="OrthoDB" id="10254310at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030117">
        <property type="term" value="C:membrane coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026739">
        <property type="entry name" value="AP_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016342">
        <property type="entry name" value="AP_complex_bsu_1_2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11134">
        <property type="entry name" value="ADAPTOR COMPLEX SUBUNIT BETA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002291">
        <property type="entry name" value="AP_complex_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="8">
        <location>
            <begin position="213"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="661"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="667"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="677"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="699"/>
            <end position="710"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="750" mass="86446" checksum="61A126F55F2132D6" modified="2017-01-18" version="1">MMDSFIKYKAFELRQEYEFILKDSNGIPQDTVRLIHWLKKIISNFAMSNHLEMSHLYLEVYDIWRFHSNGESVSKKHSHNKNHHKVIMGICHHYLQLMTSPQVIKPEVITAVVDQTRLNLAHALAEKDIALFEQSFKLCLQLNNIPGFLNEAERIVETKLFSEKVQDEFGFRICTESLILLPSILKRNPQVFSILWGNFTRKELSPTQRIVSLNTLYQLSNNDVKLEYMDVVDIFELLELLNEWDKSVILTNLPISFVPTTHAQVYKLINLTMRQLSSLNTSNVMNSLKFIMYLLNYVDEIDLTEVKNLSNGLVSLLNKPNELKFLVLRNVILLLLSRQPAFFQGKIDISYFFIEIDDPIYIKDTKLEILYLMANEQNISTILDELKEYCMDTDVSMSRKAVRAIGNLAIKLQNEVGVRESVQHLLDLLEFFANNDYIIQEIISVFKNIFRKYPQYGTSKIINQLIQNIDNIDENESRSSMIWFLTNYAHYDPAKYFRILTQYYYLNFQAFALQPFDVKCSILNSVMKFFLNTVQKKTSSLDSNEVLHVCNTVLQNCVDKVANPDIRDKALLYQRLLSTLSGAAGITMSIEDVSKIVDCDLPLITLNDQLDPVILESLELEIGSTASIYLKPVEQIFRSDKPKYLPASDILHRDKRTLHITGSSRPTELHSXELRNGKSNTSSLFESPFNESPTETRFSNSNRSRSSSSSGAGKNLSDFDKPATKVNTVKRKKTLVRKPSMLSKTFNLKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1V9</accession>
    <name>A0A1E5R1V9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">40S ribosomal protein S17-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g4169</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1V9"/>
    <dbReference type="FunCoup" id="A0A1E5R1V9">
        <property type="interactions" value="1169"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1V9"/>
    <dbReference type="InParanoid" id="A0A1E5R1V9"/>
    <dbReference type="OrthoDB" id="1727351at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.20">
        <property type="entry name" value="Ribosomal protein S17e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001210">
        <property type="entry name" value="Ribosomal_eS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018273">
        <property type="entry name" value="Ribosomal_eS17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036401">
        <property type="entry name" value="Ribosomal_eS17_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00833">
        <property type="entry name" value="Ribosomal_S17e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116820">
        <property type="entry name" value="Rps17e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00712">
        <property type="entry name" value="RIBOSOMAL_S17E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="111" mass="12802" checksum="A5C3ECA532E126D1" modified="2017-01-18" version="1">MDFQVNKRLCDDIAVIQSKRLRNKIAGYTTHLMKRIQKGPVRGISFKLQEEERERKDQYVPEVSALDLSRSNGVLNVDKQTSDMVKSLGLKLPLAVSDVSAVRDRRYRKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1Z5</accession>
    <name>A0A1E5R1Z5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS19m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g4284</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1Z5"/>
    <dbReference type="FunCoup" id="A0A1E5R1Z5">
        <property type="interactions" value="150"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1Z5"/>
    <dbReference type="InParanoid" id="A0A1E5R1Z5"/>
    <dbReference type="OrthoDB" id="2043at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.860.10:FF:000001">
        <property type="entry name" value="30S ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="entry name" value="30s Ribosomal Protein S19, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_uS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_uS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_uS19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_uS19_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="RIBOSOMAL PROTEIN S19P FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880:SF8">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US19M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="Ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ80914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="91" mass="10331" checksum="6064F117F5A70055" modified="2017-01-18" version="1">MRASLQLLKRSVWKGPNIVPLPIKEAMQKGTSVRTNARAATILPQFVGLKFQIHNGMEYVEVAITEDMVGNKLGEFAPTRKRFRYTLTKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R218</accession>
    <name>A0A1E5R218_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4380</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Non-catalytic component of the proteasome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R218">
        <property type="interactions" value="1207"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R218"/>
    <dbReference type="InParanoid" id="A0A1E5R218"/>
    <dbReference type="OrthoDB" id="10248542at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03760">
        <property type="entry name" value="proteasome_beta_type_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000014">
        <property type="entry name" value="Proteasome subunit beta type-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016295">
        <property type="entry name" value="Proteasome_beta4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF6">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001213">
        <property type="entry name" value="Psome_endopept_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 4 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR001213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ80946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="276" mass="31387" checksum="C922EBBD3CD6CEC5" modified="2017-01-18" version="1">MNHDPYHWGRPTDEMYGQYNHEIANAHKHYSNTATDHXSPKMNTTQPIVTGTSVVSMKYDNGVIIAADNLASYGSLLRFHGVERLIRIGQNTIVGVSGDISDMQYIERLLDDLQVENNYDNSYVDNDELLQPSYVFEYLANVMYNRRSKMNPLWNAIIVAGLEKNTTPFLRYVNLLGVQYGSPSLATGFGSHLAIPLLRKVVDDEKDVEKVSLETAKKTILDCMKVLYYRDTRSSKKFSLAVIDKDLGLIYEPEKEVEQMSWGFAKDIRGYGTQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R285</accession>
    <name>A0A1E5R285_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pre-mRNA-processing factor 19</fullName>
            <ecNumber evidence="8">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3941</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Ubiquitin-protein ligase which is mainly involved pre-mRNA splicing and DNA repair. Required for pre-mRNA splicing as component of the spliceosome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the WD repeat PRP19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="8"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R285">
        <property type="interactions" value="437"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R285"/>
    <dbReference type="InParanoid" id="A0A1E5R285"/>
    <dbReference type="OrthoDB" id="687049at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070534">
        <property type="term" value="P:protein K63-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000027">
        <property type="entry name" value="Pre-mRNA-processing factor 19, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038959">
        <property type="entry name" value="Prp19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013915">
        <property type="entry name" value="Prp19_cc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43995">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43995:SF1">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08606">
        <property type="entry name" value="Prp19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="U-box" evidence="9">
        <location>
            <begin position="4"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ81015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="523" mass="59676" checksum="4956E7B04EBCC03C" modified="2017-01-18" version="1">MAVDWKCAISGAVPEEPVLSKVSGCIFEKKLLVEYINMYKKDPVNNQPITYPKDVVVIKNPENPVSVQQGLAAASNYSIPTLLHSLKNEWDAVMLENYTLRQQLQRCKDELSESLFKYDASVTVASNAMKERDLYKNELARLTKNTEKDYGELIYNASAKYLGLYKKLMKRKGKGNPYLEASADSXTLKKRSICDVTNFFTTDYARSPLLASQTEANLSKLEVLNVFTEELQQSPAFDWLTYKADIVQVYEWENINKILVSGNGKLRCMDFWTAKESLALRVWEIDMSLHSGNKFWYLPHLFEDRLYVLDAQSRTVQLKSFVDPNDNGLLLQLDDSQTYEFPIDEEDTAEFSCDLHKDGMLFALFNYKTSTLEIYDLADTLNNAPAQTLEIKRATSKVSLKFSENGYFLFLNYNQECSVIDLRKDLAIAANLSFAEEAETGQNFSVASDISGLNLFVKSAQSTITWFYKQGKSEWKKKLFNVDTLPADTNPSNCKLHLAQSSYKSSQNERTMLVMGTQMYEFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2B2</accession>
    <name>A0A1E5R2B2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Lysine biosynthesis regulatory protein LYS14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4232</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2B2">
        <property type="interactions" value="63"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2B2"/>
    <dbReference type="InParanoid" id="A0A1E5R2B2"/>
    <dbReference type="OrthoDB" id="424974at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF49">
        <property type="entry name" value="LYSINE BIOSYNTHESIS REGULATORY PROTEIN LYS14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="213"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="149"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="252"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="429"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="13"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="150"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="181"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="278"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="429"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="5">
        <location>
            <position position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ81024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="913" mass="102316" checksum="EB300F383D49A2DA" modified="2017-01-18" version="1">MMDQTIEPKTASPGATDNTPQVAASDSDAPRNTQSILQTNTRSVDSLPIHPGQHRYTHESTDSKPFFFSPNISESPKYSIALSPAPLDTPGSTIPHIYHSNNSLADKSMTNERAGQKNDYIVMKPLSPPPPMVFGGTPTALPSLSNILMNDNSNSNSESSLPYVNNHLRSSATPLSPLPPQQTGNVNTTHGVSNDLALKNPPQKGKRCYSRNGCQACKTRRQKCSEEKPTCWNCRRLGRECIYIPKLKNKKRRSKITDSENSKSEEQTKSKSEEQTKSKSKTKKPKVTKKKPKSSLSGNNTSXLAENSAQIFDLKNANTLVNDDXNNIPGLFDDLDTNDVNLLLQDLNDIVSMKLNDSNITLDEYEIPGLEFFNVNIDNNAGSTNSGSKTANNTHTTPFKTLSLGHTAPFTKGTPATFSKTEVKAELQSPSISPSSLTTLPHMGSPTXAATHSPEVPVSVLVDSPITFNTQLDSFKLGGKHDEYLKIFYYDCMDSIAPFAQDQINPLRDTLLSFAKNEAYLLSAILATGASISFRKSKLENDEKLYCQYLTHCLNLLSDQFKEESNLLNNIEPVILTVIMLAWDCVYTMNSQWRSHLKGVTKLFKKINLSSSKVLNLAKCWFKVMETFANISTVLGGALLDEADLDIIFDPYNEQYIESLKFLNVITPVKEFNLIRGHKEDFDLVIKEVIKMLNSIRHSEKNYFNTKGIFNKDLDYLLWSPSSNGVELSYFQIQKILSEIDRQLEYVFIDKSGVIPLSNPNHPKNSGIVDNAIDMVKLKTNDEVIAISWYDISHQTQVLSFLLIVLLKLLGIPKESIMIQQVVEKITGFFQFLDSDSPPKNLRTCYCNFAILIAGLNAQDEKTRNIVKKYYKVNGDRFQRLTEHNLNRLEKVWYGVKDSGEKYKLENQDVLTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2C3</accession>
    <name>A0A1E5R2C3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CCT-theta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3919</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2C3"/>
    <dbReference type="FunCoup" id="A0A1E5R2C3">
        <property type="interactions" value="1610"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2C3"/>
    <dbReference type="InParanoid" id="A0A1E5R2C3"/>
    <dbReference type="OrthoDB" id="1748577at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03341">
        <property type="entry name" value="TCP1_theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000008">
        <property type="entry name" value="T-complex protein 1 subunit theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012721">
        <property type="entry name" value="Chap_CCT_theta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02346">
        <property type="entry name" value="chap_CCT_theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ81056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="589" mass="63969" checksum="76E89CBAA2F205A3" modified="2017-01-18" version="1">MSMKLPQNPNAGLFKQGYNSYSNADGQINKSIAAIREIHQMCLTSMGPCGRNKVIVNQLGKIVITNDAATMLRELDIVHPAVKVLVMATEQQKIDMGDGTNLVLVLAGELLNVAEKLIALGLSPIEIIQGYNMAKNFAIKELDSMYVNEISDKANKEELIKMIKPVIASKQYGHEDILSDLVSEAVSYIGGNSKSGFNVDSIRVVKIMGGSLFNSQVIKGMVFNKEPESHLKSLPNNEKHKVAIFTCPIDIANTETKGTVLLHNAKEMLDFSKGEEQQLDEIIKELADSGVKCVIAGATVSDLALHYMNRYNLLVLKIGSKFELRRLCRVCGATPLARLGAPTPEEMGVVETVKTMEIGGDRATVFKQESNEQTKCATIILRGATQNNLDDIERAIDDGVAAIKGLMKPDTDSGKLLPGAGATEIELVSRITKYGERTPGLLQLSIKQFAKAFEVVPRTLAETAGLNINEVLPNLYAAHANTKNNEDSIDYDEEDRKILNENVDGNGNNSNNSNALYQGIDIDGVYPDGIKDVREDGIYDLLAAKKFAINVATEAANTVLSVDQIIMAKRAGGPAVPKGPKPGNWDQDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4L8</accession>
    <name>A0A1E5R4L8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Essential for maintenance of the cell wall protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3748</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4L8"/>
    <dbReference type="FunCoup" id="A0A1E5R4L8">
        <property type="interactions" value="1213"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4L8"/>
    <dbReference type="InParanoid" id="A0A1E5R4L8"/>
    <dbReference type="OrthoDB" id="1936594at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044244">
        <property type="entry name" value="TTC27/Emw1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16193">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16193:SF0">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184602" evidence="4">
        <location>
            <begin position="18"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="655"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="689"/>
            <end position="722"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ81821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="920" mass="103982" checksum="36D8F8547165509D" modified="2017-01-18" version="1">MDILFHIHLLLSTPIATFDKAVNDANGSELAANTYHQTTKLILEGQSANFVSNWGKEYNVQGLVSELEQLVLNQSAIEASQLQTAVQQKITGYLKENFVCNDVNSSLLLAIALLQTFIQANYTGPAPNLAFPKTSDRLKQTVLQLLSCSGQPAYELCDYPICLLLSLILVDLLTGDVPNNAESGVAIPIIDRSSLVPFQSICLWWKARAIMCQLSLLHEPAGDAPVVASSIYSSIDLVASILADQVKTLVSENTQRELYTIFYLENAKCSLAINTEHFCLPSLIKAQKLTNFQFVLTGARAKRTKYQSVAHANLVVLAKSDDSLIPTDTDDAKVGNRTDLPQSLDLNHEMLLEKPVFESIGAEPLDQQIYKKSKITDENGFNEDTLLPTALRQENIPILLRELDPNSQPKLNNLDDIQLLLRLYTLRQTSPAKDPLVEIELSALVTRLIFDGQDTDTKDWSIFSRSLWERSIIETTKAKTVERGLLQMQALVEELGLKIKTTSLPTDADAENSSSDNGMTRMKYIHQLPFLPRWNLDATLAEKYMSMGILRSAVEIYERLQMPCEAALCYAAVGDEKEAERILEQRIITNPTDCRAFSILGDIKQDPELWEKAWSLGKYVNAKNSLGKYYFNVKKDNVTALKHLNDSLKQYPLSFETWYFYGCVALEHGNMTVAAEAFSRCVSLEDDHALSWSNLSAAYVELGKLKQAHNCLQKAVKSDSQKNWRIWENYMMVSMKCNEWDDVLLCCQNLLKLKKDNNSMSIIDLPVIEKLVELLITSEYDPENQRLTHFQTSCIDFVCVQLPQVITNEPRCWKLVSKVELWRKRPWAALENVEKAYRSILHNPDLDVDEKVWNDTVDACEDLVAAYESLGEMEGRMGDLVCKDWKYKARSTIKSLMSKGKKTWDGSEGWERLLEMRNDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4N0</accession>
    <name>A0A1E5R4N0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Putative vacuolar amino acid transporter YPQ1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3797</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the laat-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4N0"/>
    <dbReference type="FunCoup" id="A0A1E5R4N0">
        <property type="interactions" value="321"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R4N0"/>
    <dbReference type="OrthoDB" id="8048523at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015101">
        <property type="term" value="F:organic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034488">
        <property type="term" value="P:basic amino acid transmembrane export from vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034490">
        <property type="term" value="P:basic amino acid transmembrane import into vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000011">
        <property type="entry name" value="PQ loop repeat protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000012">
        <property type="entry name" value="Vacuolar membrane PQ loop repeat protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF35">
        <property type="entry name" value="VACUOLAR AMINO ACID TRANSPORTER YPQ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ81844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="326" mass="35893" checksum="A965C90833699B5D" modified="2017-01-18" version="1">MLSPVPIVLNAQTVSGITGSISIACWIVVFVPQIYENFYRKSADGLSLLFVVLWLAGDVFNVIGAVMQKLLPTMIILAAYYTVADILLLVQCLQYDNEDTKLDPVHLSPANPLSHHIVEVFDETQPLLTSASAGNGESSASLEGESPYTSSTSLVSSTSIAQTYNSDSIFIRNLKEIMVVLSVILGGFLFWYISYCNNPKTPKPTPPEELTFNITAQVFGYLSAILYLGSRVPQILLNFKRKSVEGISFLFFLFACLGNTTFIISVLSISMHPRYLLVNASWLVGSSGTLIMDFIIFTQFFVYGTSNSDKKDTVVNTQDIENQVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4U3</accession>
    <name>A0A1E5R4U3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitin synthase</fullName>
            <ecNumber evidence="2">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g3782</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4U3">
        <property type="interactions" value="85"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4U3"/>
    <dbReference type="InParanoid" id="A0A1E5R4U3"/>
    <dbReference type="OrthoDB" id="370884at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054295">
        <property type="entry name" value="CHS4-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF16">
        <property type="entry name" value="CHITIN SYNTHASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03142">
        <property type="entry name" value="Chitin_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22997">
        <property type="entry name" value="CHS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="488"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1024"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1049"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1075"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin synthase 4-like" evidence="12">
        <location>
            <begin position="396"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="94"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ81936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1183" mass="134365" checksum="D6422001C2706DD9" modified="2017-01-18" version="1">MSNKNIAENGLDMSSSGKSENVTGIDDAGQALDCDLEKCSKINSKQSDVFESKMRSLTHPDKSTLLDPNNPHFYYARKVMENADHLDVLPSASGFEPNSALKEEPSKSFKKRKLSKQQFFSNDKSGSINKLKNEVLSEQSSDLGSCSKNDLEKSRASNSKKLDLENKSFLSSNLLVHSKKSVSRMHKYTENNKHKRVFGLWQLYCYLITFWAPNWLLKWSGMKEKSRRLAWREKIALISLILYCGGLVAFLTFGFSRTVCHPGALRLKHDQIDNTYLVINGKGFDLKEYKHPEALNIGKDSQVLNPPTSAAGKDASFLFQNVNGNCKSLILPRENCTIPMDAEKNIAWYFPCKMKAIDGSTKPNFDQKSDAYEGYACHTSWLSRDVYYTFLASVDVYYNWEDLINTTRNLVVLDGNVLDFDILDWFERDHLTYPMEFDFLKNAKLQGYDISVILSNYGRDKIISHCLTEILKVGVIDSESVGCISSKVVLYLSLIFILSVIFVKFFIACYFKWVVSRKQGVCKLDRKKFNELSNKIEDWSNDLNDIGPLEQPKVACSVTENNLFNTPLNYNKIEKCGMTTMTTQAVLFERKKDDTNVRQTELPEEIDQQSFAWLEKKHKLSWTHPHVVSQPRADFMPFNFPLIHSICFVTCYSEDEQGLRTTFDSLSVTDYPSSHKLIICVCDGVIKGSGNDKTTPEIVLGMMCDFLVDPEVVKPYSYVAVAAGAKRHNKAKIYSGFYKYDETTIPLLKQQKVPMVCIVKCGTEFEKDAQKPGNRGKRDSQVILMSFLQKIIFDERMTELEYHLLKNIWCLTGLMSDFYETVLMVDADTKVYPDSLKHMVAEMCKDPSIMGLCGETKIANKSDSWVTAIQVFEYFISHHQSKAFESIFGSVTCLPGCFSMYRIKSPKGSNGYWIPILANPDIVERYSDNVTNTLHKKNLLLLGEDRYLSSLMLKTFPSRKQVFVPKAACKTLVPDTFSVLLSQRRRWINSTIHNLMELVLVNDLCGTFCLSMQCVIFIELMGSIILPLAITFTIYIIIYSIVSEPTPVLTLILLAIILGLPGVLIVITASRXSYLVWMLIYILALPIWNFVLPTYAFWKFDDFSWGETRLVDGENAVFGASDASVDPKLNKTTCENASGEQIKISKKIASSHDEIEGEFDYSGIVLRTWLDFAKRELPKQGSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4Z4</accession>
    <name>A0A1E5R4Z4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Vacuolar protein sorting-associated protein 68</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3756</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0220 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4Z4"/>
    <dbReference type="FunCoup" id="A0A1E5R4Z4">
        <property type="interactions" value="198"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R4Z4"/>
    <dbReference type="OrthoDB" id="3970977at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007919">
        <property type="entry name" value="UPF0220"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13180">
        <property type="entry name" value="SMALL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05255">
        <property type="entry name" value="UPF0220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ81951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="215" mass="23195" checksum="5A4B1C14FD4E3476" modified="2017-01-18" version="1">MSSSLPFSNGHDAPRYASAFHHPIFKAPFKLVLSFFSTSAKPHDLLVSLSSVLFALALLFFLDAVMYSHRMNASDVVHISFIDWVPFLCSLIGLCIVNSVNKGYLVGNGENGASGSNFLVGGGGVGGGDDRVMREVKIFLFLGFAFLGGGCSGSIVILIIKYVNKGIVEWPTVGMGVDCVVSNGLVLLSCMVLWIAQTARYNSDFDEEYNYSLTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5B1</accession>
    <name>A0A1E5R5B1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3607</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the FNT transporter (TC 1.A.16) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R5B1">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5B1"/>
    <dbReference type="InParanoid" id="A0A1E5R5B1"/>
    <dbReference type="OrthoDB" id="3973045at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015513">
        <property type="term" value="F:high-affinity secondary active nitrite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015707">
        <property type="term" value="P:nitrite transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000011">
        <property type="entry name" value="Formate family transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000292">
        <property type="entry name" value="For/NO2_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024002">
        <property type="entry name" value="For/NO2_transpt_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00790">
        <property type="entry name" value="fnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30520">
        <property type="entry name" value="FORMATE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30520:SF6">
        <property type="entry name" value="FORMATE_NITRATE FAMILY TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01226">
        <property type="entry name" value="Form_Nir_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01005">
        <property type="entry name" value="FORMATE_NITRITE_TP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="91"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="233"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="307"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="404"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="528"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="641"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="316"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="641"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ82097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="693" mass="76441" checksum="6F92904FE0508985" modified="2017-01-18" version="1">MVDDTYYITPHETALAVVATAMKKARLRLDTLIINSTVGGLLFSAGGMLYLYSRAENPAMAETNPGIVNFLGAITYGIGLFYVVINGADLFNSNILFFSVGFLRGAVTICDLLVSWSISWLFNLGGSLFMCYVICYLSGVATSENMVAGSIYVSTSKASFSFIQTFIKGIAGNFCVCLAVYLQLMAKPLHVKYILITLPIFTFVAMGFTHVVADMYLVPMGMMNGAPVSVGKYIWKLLIPATLGNMVGGSFFAAVIPFYLHLFVVEQDRKKLELPDYDARDEQPELNMDSRVVRTDPANIDDELDSEVEDLDQNEKGSSVATDSFGSGETYTDEEKTNMQPQRYIPRTRSSVFSSALNKTKSAGFKKRAMRSPAGVFPVQGMGDPLLREMSIADPSIVEKVKSKLSAQESRKEPNEQTLLDAEDSANHARLVSEDDIYDPRAAGPPSVENFDAYSIPSNYEPPLSGTISSPQKQLTNPHVPTARLNKIPTFKDLEEEEEQEYKRGGGYDVNENKLGTKLEKALSHIAGKERSSSKSIKHSQASPVLPLTHQDTEAFSKRTTDARVTRAHTEIQKPHPALLKSLRKSFNMSSASISANELQKRLSEVGVTQRAARAADNIAGLENFQDMDLPVSTSPFYTLRRTSTSTTASSRXAETEYDQNSYKNSRKARSPSHLKNTFLYDNDDMEEQSIAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R679</accession>
    <name>A0A1E5R679_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA primase</fullName>
            <ecNumber evidence="10">2.7.7.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3661</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the eukaryotic-type primase small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="10"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R679">
        <property type="interactions" value="1292"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R679"/>
    <dbReference type="InParanoid" id="A0A1E5R679"/>
    <dbReference type="OrthoDB" id="19606at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04860">
        <property type="entry name" value="AE_Prim_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.920.10:FF:000003">
        <property type="entry name" value="DNA primase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.920.10">
        <property type="entry name" value="DNA primase, PRIM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002755">
        <property type="entry name" value="DNA_primase_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014052">
        <property type="entry name" value="DNA_primase_ssu_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00335">
        <property type="entry name" value="primase_sml"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10536">
        <property type="entry name" value="DNA PRIMASE SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01896">
        <property type="entry name" value="DNA_primase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56747">
        <property type="entry name" value="Prim-pol domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 10" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3 10" id="KW-0639">Primosome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ82043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="414" mass="48641" checksum="A10141EBEF0F771C" modified="2017-01-18" version="1">MSVLNQQDMQFYYGHVYPYKPIYQWLNRSFSDNGTRNMINREIAIEFKTGAYGRYFSFPTLKLFKEKMXNDIPNRFEIGAIYSSPPKKKDSVLKNSMKPLEKELVFDIDMDDYDGYRTCCSGAQICGKCWKFIEIAIKVMNITLVEDFGFEEFFWVFSGRRGAHCWVSDTRSCLMNDLQRRNVLDYLNVVKDRDIEKKIGMIHRPLHPFIERSLTILKQDFVDIILREQDPWRDDEQAIKTLLAGFYDKNLISHCKKYWLENPGRSSEVKWCDIDFIAARALTYGKKPEYIEKLKHCKEDLILKTLYPKFDIEVTKQMIHLLKAPFCIHPKTLNVCVPIMTHNKGQDLQGXSIQDFDPTECPKLNDLINAMSENGMDISTTNLRPYVELFNKFTQTYVKGQVSVKREHENXLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7G9</accession>
    <name>A0A1E5R7G9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">General amino acid permease AGP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3360</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R7G9"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF17">
        <property type="entry name" value="GENERAL AMINO ACID PERMEASE AGP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="274"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="421"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="443"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="485"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="522"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="7">
        <location>
            <begin position="86"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="8">
        <location>
            <position position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ82860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="585" mass="64150" checksum="22393B92AB6B250A" modified="2017-01-18" version="1">MSSIKQTNGKCSNPDDTGSAKXEXXKXXKANVQTMVINPTDQSSGDHSNTHNAFQSSNSMLTIEDSINNSILEKKQALKETXKPRHTKMIAMASGIGTGLXVASGSQIAAAGVGGTLISYCITGLMNILMFYSCSEVVVAFPTINYTQYGKKFIDPAIGFVVSWIYCVQWMTMIPLQLITASMTIKFWTESVSPSILVTIFYFLVLGISIFGAKGYAEAEFXFNXCKITMLLGFIILGIMLGVGKVGTQPNHFDKSLNSNYYKNPGAFNKTNNVFKAMCSTLLASTFACGGVEFVALTIIEQDKSNISKSIRSIGWQVIQRMVVFILIPIAVIGLLVPYNSSELLGSGSESIHASPFVVAVTSHGIRVVPSIINAVIVIAVLSVANSAVYSSSRTLRTLCLQGFAPRWFEQLDKSGRPRRALYVTAAVGLLSYMAEYKDQELIFNWLLSIGGLSSIFIWTLICVSHVRFRAAMKAQGCDLSQLGCVSPTGIIGSYVSIFINVFIVCASFWTSLWPLGSKGKIDVVTCLQVILAIIIAVVLYILYKTFTKTWTKPYLTAKEIDISSDRSIYYFDEVLQKKMRFRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7M7</accession>
    <name>A0A1E5R7M7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GTPase-activating protein BEM3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3351</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7M7">
        <property type="interactions" value="201"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7M7"/>
    <dbReference type="InParanoid" id="A0A1E5R7M7"/>
    <dbReference type="OrthoDB" id="3972322at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005933">
        <property type="term" value="C:cellular bud"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050729">
        <property type="entry name" value="Rho-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF129">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN AT 16F, ISOFORM E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="761"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="4">
        <location>
            <begin position="1063"/>
            <end position="1266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="116"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="293"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="481"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="551"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="874"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="122"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="481"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="551"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="578"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="939"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="954"/>
            <end position="974"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ82919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1266" mass="139089" checksum="D56C6C7DF6742593" modified="2017-01-18" version="1">MNFAGKENGIPFSASQEKECLHPDANSESATSALDLLNHYIESTEQRKVDLGVDYQHDTSPKQLQQENTLLKAQVNKLKSELLKHISLIELKTIMDEVSVSSTNASTELGIKSKASNQSFLSSTASPSSTVAPPRSLNRNHKAKNLPLDLTDTDDGSISSSIELSPDKSVIPELARESKLQPAISPLGTTMHIPPPPPSLNFVRKSDSNTSLHGALQEDIQNSKPAVSAIYKSSRIHISSSPKPQQPVAQQEITVSAASFNNDTLQSSSTPTRMKIVEDFSNLMVPQSEASAQYHVPSNHSTVRPQTFLSPGESPNRSNRLTSVVGNLLHSPLRPPVSVAGIKEELEENEQTCSFESNVPNLSHDLNDHSSVNYDKNSIISFDSSTANKVSKPLGHQFSKDSLLRSPQISGNRKFSNDSSNLNQTHLMDQTLSSAVMSQNSAGSSALNTPKKGKAPGFFVSNSDNFKEDSLLASPILINKQHQSASNRAVQRSTSSLAEKRSLGQDQSGARPVVRQLLSGKTTSSTTSFGANNVCTSDSLSISSYSPNPQEFNAKFSNSQMPPVPQAVSQSQNQVPISNFSPSSNYSPQSSRLXSPRGKPVKSSVPLFIQPEDLNTVKIHVIGTLYHDLKINNALYTSNDVASISSHTSSDENYSSRVLFAVLDRQTSKKIFEFSKTVEQIYELNQSLGSYMNLLSLPPLPDRSLFKTNVPSKVNMRKEKISDFFLSLFNIPVIPSMGLLKLAEFISTNTVTSGMGLVADNGLLEAWVLVRRPKTIGSSGGWRIKYAVVTHKGSSIHLYNMDSELAEEIRLPQSTIEIHPNIPEDKYGTYNGFLLHEPKKTGLSSSNKYYICLESAKEREEWVGLLSRLTVVPAGPKNGVQKKANNQRTSSNNNSSYPMFDNTEDVSSAGKGQTSNEAGREDSSTYGESSIISDDHSSNKSTLLDASNNNDTKSLGSHSVLSGSSQNGLSGNTXSDRETKRNRMRSFFPFTKKTSHQGPDGMPASPRLTNQSTMETQLDSFMESPTREPFENNSTISKTLEMMNLNTKVNNGKVFGNSLLGALELSSQIYQETHRIPTVVYRCLEFLYKNHAIEEEGIFRLSGSSAVIKNLQEQFDKTPDISLCSYTELHVDVNVATGLLKLYLRSLPHFIFGDENFDKFKQVLEENSKDDHAIALKYKQLCXEHVSKEDFDLMFVIFELLVTISRNSDVNKMTLRNLCIVFSPTLNVGVNMLQPFIADFGCIFKNKEPLDASQRSALNVNIPTVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7S1</accession>
    <name>A0A1E5R7S1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3429</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R7S1"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="107" mass="11919" checksum="AD0C789C8F601783" modified="2017-01-18" version="1">MHDIVASTLAEMEVTNQIFEDHFSRAEKLDFFVAEQSLVDYPKSVQAKNDLSKLEDLSDLAKTAILASAFVQNNKELVENFEADTGETFGXNPATQDNSHQTSSLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7V6</accession>
    <name>A0A1E5R7V6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ADP-ribosylation factor-like protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g3251</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7V6"/>
    <dbReference type="InParanoid" id="A0A1E5R7V6"/>
    <dbReference type="OrthoDB" id="414781at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001317">
        <property type="entry name" value="Putative ADP-ribosylation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45909">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45909:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="161"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="420"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="487"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="632"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="763"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="858"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="20"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="164"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="192"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="223"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="269"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="315"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="420"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="487"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="632"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="792"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="816"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="867"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="905"/>
            <end position="912"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="912"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="935"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="960"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="1019"/>
            <end position="1022"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ82986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1084" mass="120493" checksum="F19B3F80B0F7E50B" modified="2017-01-18" version="1">MLNTSQPSPKNAETPPEMKXSQKESSNDKLQHETQHETQHETQHETQHETQRETQREQNGKIVINPSTTTTPKLISRANILPRRILQKPGSQTALNQVQQLEVVDNSQKRKRLKELGLDCISPGLPSTKIDNQTYKSLKNIVKEQFQLIKDKSGASLLTGNATSLPSSSSSLXSSSSLSNAKVVTEGENKEIQTSSNEVNTKNNTEDEDMKVQENSTDAEEKNTEEEEEGEEEEGEGEEEGEXEEGEGEGEKEQEEGEEGEEGGKDNENTDXDQDDEGLNGEGLDEPKETNEQQQQAPAQHEHVFANDDQNNHGPPAATPSSSTPLRFKFFPSSTNHKSLKRRKIPPPLNIHQPNYTTENAAAPSNLGPSASEDXHAENCDTKANNPLKSAKLGKKSHKVTKPATAAKATVRYAGYTPKTLNTTASTNGAQNMFSNQQPNQPRSQAPNSFPPPPLTSLYXXPHPYASVNHPLSATIASAAINSYHLANTNDSHPSNVTPSAVPPESENAEKNVSPGNEPHFQAYXSYMQQQAMFAATPYSTLPYNPLFSTAMPHLPYQQQQQQQQQQQQMMXHGLGQLPLYYGSPVVSQQMLYPGQATAFNNGGLLPGGVPNPGLYPPQFSATYPVAPQESLPLQRSPNVSEASFEAAKHHKNKGKVTDRTTTLGRKSQDSEKLGXLIHSENISGHVQVLEDRFEFEFESGADELQGKNREEICKLNKRKFLKICEKIWDESYWLLQKRLQYSNPTPTGLQEKHTLVHKKCSNGETESCDAKTDPSNSTSDSDEDVAESTRSPSAQSVSGSKSPSKSLEDISSTPSEKEAVSNAASKDETEHXNPTMEIKGFRTLTQKDYESLWNNKKETQKKKQNKNNTNPNANSTANKTMFHLAKGLYTNYNKKPQYSVLLLGLDNAGKTTFLENCKKQYGQIYKDFDKITPTVGQNVAVIQLLDKEHTFLKFWDIGGQKQLRRLWPSYYSQCHGIIFIIDSSDKTRLQECQETFLNMVISNNNLEDSGIPILMVANKQDREDAMEVSDIKQIFNKIAEHLDASDSRVLPISALTGEGVQDSLDWLVTRLKRNKKTRPPIYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7V9</accession>
    <name>A0A1E5R7V9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 22">Folylpolyglutamate synthase</fullName>
            <ecNumber evidence="6 22">6.3.2.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20 22">Folylpoly-gamma-glutamate synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19 22">Tetrahydrofolylpolyglutamate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">AWRI3579_g3414</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis. Required for methionine synthesis and maintenance of intact mitochondrial DNA. Involved in telomere maintenance.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21 22">
            <text>(6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n) + L-glutamate + ATP = (6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10580"/>
            <dbReference type="Rhea" id="RHEA-COMP:14738"/>
            <dbReference type="Rhea" id="RHEA-COMP:14740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:141005"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="22">
            <name>a monovalent cation</name>
            <dbReference type="ChEBI" id="CHEBI:60242"/>
        </cofactor>
        <text evidence="22">A monovalent cation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 22">Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 22">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.17" evidence="6 22"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7V9">
        <property type="interactions" value="652"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7V9"/>
    <dbReference type="InParanoid" id="A0A1E5R7V9"/>
    <dbReference type="OrthoDB" id="5212574at2759"/>
    <dbReference type="UniPathway" id="UPA00850"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.10:FF:000009">
        <property type="entry name" value="Folylpolyglutamate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023600">
        <property type="entry name" value="Folylpolyglutamate_synth_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136:SF5">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038895">
        <property type="entry name" value="FPGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01011">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01012">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 22" id="KW-0436">Ligase</keyword>
    <keyword evidence="15 24" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 22" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="24">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="348"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="25">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00025142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00030592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00030876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRNR" id="PIRNR038895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR038895-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR038895-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="OEJ82996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="508" mass="56727" checksum="A587439478DD2A88" modified="2017-01-18" version="1">MTGNRTYTDAINSLNSLQSNFADLQAVRDTGKSQKNLMNIWEMQEWSRRIGYDSIPKYYDGLNVLHITGTKGKGSTGAFCQSILEQYAKNXTMPLKIGMYTSPHLKSVRERIRINGNPISEELFTKYFFQVWDRMDATSSDESKFPHMTLGKKPGYFKFLTVLSFHVFVQEKCNTCIYEVGIGGEYDSTNVIENPTTCGVSLLGIDHVFVLGDTIEKIAWNKSGIFKKNAPAFTISGQPTQGLQVLKERAVERETTLTVVPNFSQLKNVQLGIHGDFQITNASLAAGMCAAHLNKIGHPEAPKLPASTAWDNFTLPEKFVQGLVHCKWEGRCQTVQSPQHANLRWYLDGAHTKDSIEACSSWFAQVITAKANAEKDYKVLLFNQQTRDASALINVLHNVITIQNKIKFDKIIFSTNVTWGSGKYDADLVSMNVSKQSVDALEVQKNLKESWEKLESNADIEIFHSIEDAYKTVSNIAAGFQNQNRAVDVLCTGSLHLVGGLMVVFDGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R8L8</accession>
    <name>A0A1E5R8L8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Reticulon-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3218</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Endoplasmic reticulum membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R8L8">
        <property type="interactions" value="262"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R8L8"/>
    <dbReference type="InParanoid" id="A0A1E5R8L8"/>
    <dbReference type="OrthoDB" id="3971976at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003388">
        <property type="entry name" value="Reticulon"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02453">
        <property type="entry name" value="Reticulon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50845">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Reticulon" evidence="8">
        <location>
            <begin position="29"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="220"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="225"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="241"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ82873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="254" mass="28856" checksum="0CB05ADD43C7F8AB" modified="2017-01-18" version="1">MSTXPVNPVQPKGSKESKNCKNCNCSIQWCSILKWENPVETGKIFGAILLFLILIKKIQLIHVLKFFYSVVFTFSIVEYGSKTLLYKNEGKGLITKFVAPKECFNIVGFLKPKVDQFLIDLPKWQYNLRQITFASKPLESLKVASLVYIFTKVLKIISLYSMIVTLVFLSFSLPLIYSKNQAAIDAQIKIFLEKYEKNQKIMINNVESKLSTTPLGKYFKKHPQTSTTSTSTTTPPSVQELNEKIDATTSGFEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R925</accession>
    <name>A0A1E5R925_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Regulator of drug sensitivity 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3388</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R925"/>
    <dbReference type="FunCoup" id="A0A1E5R925">
        <property type="interactions" value="430"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R925"/>
    <dbReference type="InParanoid" id="A0A1E5R925"/>
    <dbReference type="OrthoDB" id="65716at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056751">
        <property type="entry name" value="PAS_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053045">
        <property type="entry name" value="Zinc_cluster_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31986">
        <property type="entry name" value="REGULATOR OF DRUG SENSITIVITY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31986:SF7">
        <property type="entry name" value="REGULATOR OF DRUG SENSITIVITY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24990">
        <property type="entry name" value="PAS_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="43"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="77"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="227"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="234"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="275"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ83033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="518" mass="57871" checksum="C3356DCA0AAD5C05" modified="2017-01-18" version="1">MGNPDQQEDSTIRKEVIKSELQTPNQGSPTKNLKQKSKKKFPACIFCKRSHITCGRKNEVDPCSNCLKRNIAHLCGSPAAGSNTTSTTSSAKSKTPQEMTPPLNQQSVLDNVPTAPALPHPRSSASLFQKVSAPQPIDQQQQQLAQQVQNVAQDPKYAFFDNEPLFMSSDVGLEFSSLNNFLNTLGKDPVFGESLDASIPLTNSLSQQEDIINILNSTSTALPNAQNVQKPSVSQLPHSQPLQQQHQQQHQQQHQQQQQYTPLSTFRHSAPPLPNMSQAQNGDQGLSFEASRSRGSPVPIAKLPKVTPRENFLLTAAAPTSVHGSESRLRQVINAKLEAGLLKPYNYAKGYERLQEFMDANMKAESQQRILKPLSIIRPAFRAIARGLQDIDLMVVEESFEKLLLSYDRVFSTVSTPAVLWRRTGEIYRGNQEFATLVNRDMEELRDGKISIYELLDEESCVTYWEKYGSVAFYKGTKSVLITQCVLRNSNTKCSFSFTIKRDVYNIPICIVGNFLPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R9B9</accession>
    <name>A0A1E5R9B9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Mitofusin FZO1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g3322</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R9B9">
        <property type="interactions" value="97"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R9B9"/>
    <dbReference type="InParanoid" id="A0A1E5R9B9"/>
    <dbReference type="OrthoDB" id="9984778at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008053">
        <property type="term" value="P:mitochondrial fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051646">
        <property type="term" value="P:mitochondrion localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000638">
        <property type="entry name" value="Transmembrane GTPase Fzo1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027094">
        <property type="entry name" value="Mitofusin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10465:SF0">
        <property type="entry name" value="SARCALUMENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10465">
        <property type="entry name" value="TRANSMEMBRANE GTPASE FZO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="13">
        <location>
            <begin position="148"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="376"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="380"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ83133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="789" mass="89428" checksum="FC3C8FD40265E98B" modified="2017-01-18" version="1">MSNSVQYSNGDFFFNEGDNDHSNNGRINQYVFNENRMKLLMQVKKASSILDQLNNRNGAPSLEAGGTQKSIPINCSLSDLEILQLNLQLDNHKRTKIMDLIDVPAAKNGKESSPSLDTIGSNDLTSTLFDKQVQQAKQHLVSLEKRVMDSNSKVFITGDLNSGKSSLCNALLKRHVLPTDQLPCTNVFVEVLDTHFNKNKQEEVHAIPLSKADNVSKAIKVYNQHDLTSYELFKLGDLEDLVTRNDKWSXIVVYLNDSQPGEKEKSLLHNGIADIALIDSPGLNMDDLKTTEVFSREEEIDLVVFVVSAENQLTLSGKDFVTKSSKEKQFIFFVVNKFNAIKNKERCKQLILSQIKDLSPLTYKQSDDFVHFVSSEDNLPSEKDNGNGDDDHRDDGQDPDFENLRESLKNFVLKKRSISKLLPAHTYLSKLLHDIYLISKHNKNICQEQYDDVAKTLKELKPMVTSLEKESTSKSRKADLLVEKTINEIYDTTRQNINKSIEETVFPVYTGISEMFEYINDTIKAIKSNIVESVNISERFAKEQIXTTVQEVYQLAEILPADRPVFNKDLMFQQRRHMLSKQFDVSFETKDLFQPSWGGFVNFLQWGASVDENAKLDSYAGNVNKYFKNPSLIFNSKIPSLLVYSLGGAKLIVNVLMYGASLFSWSAMKNIGSTVVLIGGSLGCAYLINDLPRALPQNLIEKYKGVLEALDYAHANSSRVAKVCRQVLKTPASEITKKFDEKLINMNQKKFELNEKMVFYKRNLTFFDELKNKASRQSAELNTINLDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAE8</accession>
    <name>A0A1E5RAE8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2564</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAE8"/>
    <dbReference type="InParanoid" id="A0A1E5RAE8"/>
    <dbReference type="OrthoDB" id="10008801at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ83872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="151" mass="16869" checksum="CCAA7188EBE7DBB2" modified="2017-01-18" version="1">MFTIATRRSLFKAPSAVLKQTTFVRNPSFFLATTSFTTSKRTFFGGNAQPSIDEKKASEAAQAFINKISQYPNVVEKFSEAQQVFASKSIGAKNFETMSAWQSLKFLLDPEIKQAIKNICDEMKKSNVNFDKEEVQSLMAAINSSMGQTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAK6</accession>
    <name>A0A1E5RAK6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Protein BCP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2713</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in nuclear export, actin cytoskeleton organization and vesicular transport.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the BCP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAK6"/>
    <dbReference type="FunCoup" id="A0A1E5RAK6">
        <property type="interactions" value="1020"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAK6"/>
    <dbReference type="InParanoid" id="A0A1E5RAK6"/>
    <dbReference type="OrthoDB" id="27543at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025602">
        <property type="entry name" value="BCP1_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13261">
        <property type="entry name" value="BRCA2 AND CDKN1A INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13261:SF0">
        <property type="entry name" value="BRCA2 AND CDKN1A-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13862">
        <property type="entry name" value="BCCIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028983">
        <property type="entry name" value="BCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR028983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ83927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="297" mass="33728" checksum="2C25CA57FBDF353E" modified="2017-01-18" version="1">MVQAVQLGQVQDQLKSRKRTQRETEDVNSSDIDISSTDDEAGEKDSDDEDEDGNDNIVNIDFDFFNANKEVDFHALKTLLRQLFGKEESNKLQLSALADLVLDACATTTIKCEGKESDPYCFFSLIDYQENKNSDYVQYLQKVDMRLKTFFQTLESAGNKKAALVLSERFINMPPEIVPPLYRITLEDASNSLQASAATSQTSRDHYDFYVFVSRKYEINFDADEDVDVVDNNTKGKRTKQATQIDYFHPEDQFLEKYAKINFDGKSNKGIIPSYIIIDHAGLVKAIDDLDAAIATW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAP5</accession>
    <name>A0A1E5RAP5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2583</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAP5">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RAP5"/>
    <dbReference type="OrthoDB" id="2103474at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025187">
        <property type="entry name" value="DUF4112"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35519">
        <property type="entry name" value="MEMBRANE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35519:SF1">
        <property type="entry name" value="YALI0C06193P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13430">
        <property type="entry name" value="DUF4112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="193"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ83967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="298" mass="34135" checksum="DE347F64421DDC13" modified="2017-01-18" version="1">MSGXVGNFVKGQIEGKMQDYGLEKWNDIAGKKLFKLKDPFRDLQTNKRLGLPRELNPSKQDEKLWRSCQNKAWRDDKCFCSYFGFYMDCCFGIGMCPLVVLIPTIGPFLMYFVHNKLIRMVQKKRPGLIDNTTLAKMHANITFDLLISLPPIIGIFFTWLNGCSTRNCVLIYDCMIRDLQAKLPQNVQQVQQQQPQFIRNNNNNKNHKYYGKENVEPSYNNNGSQRYHDHDLHSXLDVQSIPMQDFQNNEXHPSAPSKTAHTSLTPPSAAAVKESEQARTVSNRRVPPPGHNTDPLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAU5</accession>
    <name>A0A1E5RAU5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">U3 small nucleolar ribonucleoprotein LCP5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2729</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAU5">
        <property type="interactions" value="1149"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAU5"/>
    <dbReference type="InParanoid" id="A0A1E5RAU5"/>
    <dbReference type="OrthoDB" id="203440at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007146">
        <property type="entry name" value="Sas10/Utp3/C1D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237:SF9">
        <property type="entry name" value="NEUROGUIDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04000">
        <property type="entry name" value="Sas10_Utp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="369"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="407" mass="46157" checksum="0C79BA6661220A44" modified="2017-01-18" version="1">MSSTARSSKNELNNALLSINDSLTSTSDSLDNLMSILQSNNNNEDFXKLQNIIQNSTATPENKKLEKVSLLSLKNESMLSYVNSLLLLLQSKLTGPEESGNXKNIEGLQAEQEADRWNTIEQRVTLXRGIKPLEKKLNYQLDKLTRSYYRVXEDFENNTNTSSSVNGGNXRSVQNGVNEKESDSDGDSESESEDETTFKPNVNGMLSSASSKLSAKSKRSSKTPEKEEQEAKDKKEEEEEEEEEEQKGNGRYQPPKINAALPPSANSHFDDKFNAKDHKDRSNKFKLQSMEEYVNEMNDKPEWEASIGTNIVNHGKGGIKSSRRNDKENEVKDYEETNFTRLNLAKKKDKRQLKIKEIRDKVNIIGGEDFSIFNNQGSKKRSFEESTSRKNSNKKSRSAWDRAKTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAU8</accession>
    <name>A0A1E5RAU8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Golgin IMH1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2728</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAU8"/>
    <dbReference type="FunCoup" id="A0A1E5RAU8">
        <property type="interactions" value="132"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAU8"/>
    <dbReference type="InParanoid" id="A0A1E5RAU8"/>
    <dbReference type="OrthoDB" id="1926336at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000237">
        <property type="entry name" value="GRIP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01465">
        <property type="entry name" value="GRIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00755">
        <property type="entry name" value="Grip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50913">
        <property type="entry name" value="GRIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GRIP" evidence="2">
        <location>
            <begin position="785"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="738"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="768"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="772"/>
            <end position="781"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="835" mass="96517" checksum="5922C78CC944FEB6" modified="2017-01-18" version="1">MFKQLSQIGNNLKEELAKSLQDDNMNDANGGTLPEEYLQYPKEVQFKLRKLNKYENKYPLLLQAYKNEKNKNAQSEQIFTVLSEHTPIQSFDLSITPPSSTSPLGSETGNEETEEVNQETNPVAQLKQFFQNMQMKTDLLNEEIKRLTVDLNQTKKENQELKERETQFGVTELVEKNTKKGAHEDGKKETSVVTETSNAETDQLTQDKDTDEKANENGDSSFKIEKPDEGVAKTFAGNASTKTGENEEETASIEEKNKTLPTDVDEKTASVENRIDTDLQPAPAREDFQKSSEALPNSKESSPTSNEKKPKSAIQWEIKYNSLKNDYDSLNTKYETLKTNHDKSVKNLKTRTEELDNVRDMLKEVGNQLVEIKDKTKESDKLGKRVQELENRLKLETNSKKTIMNTMQTQKDDFSKKLENFNNTYKQEVQSKKEFERKFMEKDRALKYLEEQVKETNKKLSQSELDLKQLNLKHERFVKSNENAVVERDQYKKQIDGLNKAIEDKIKENGKLSERLDILQEKFDNNKNIEENSSDFVENMKRQCNELNIKLRESIRLKNSLEEELNDTMSTLQEKNKELAKMKRSINEQDMQVSNQIEDWKNKYQISVERAKSMENELNLLILTQKDELTNSKTYNDELNKKIAKLEENEQLLKKQVAHLNTSFSVTEQQSQRTSSASEVLNSTIQNLKSELTKAVKLSREYEQSNTEMKKLNQELNLKLERLSKNYKSLSAQLKSARTQDYDLAKPSPRRHSSITSLSSINDVNNSLSNFSTPSTPSETKTSSEQDYDSKLSYIKNVLIGFMEHKDQRQQLLPVVSMLFKFTNQEEQKFLTSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB04</accession>
    <name>A0A1E5RB04_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Suppressor protein MPT5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2731</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB04">
        <property type="interactions" value="85"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB04"/>
    <dbReference type="InParanoid" id="A0A1E5RB04"/>
    <dbReference type="OrthoDB" id="668540at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF80">
        <property type="entry name" value="SUPPRESSOR PROTEIN MPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="4">
        <location>
            <begin position="302"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="326"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="365"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="468"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="505"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="682"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="213"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="986"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="33"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="213"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="236"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="248"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="990"/>
            <end position="999"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ84072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1006" mass="111673" checksum="74BE7629C6905EA8" modified="2017-01-18" version="1">MRSGTNENNSLNSVLNSLDQSINAHAAAVAGGPSTNGNDTGYGPSNRNNDIDSLAYSTSMMSIVDPMQTNSSMTSINGLNYLGSTNSVNKPTHKHQLSINSLSSVSSANSIGGTNSNSLFSEPITPPLFNTNNVNNGKYAYXHGSVAPGMGQYYQPQQSQPSTYYSLQNSNGNQTTYNTSTFASGFNSQLNNKHHQKAASLDMGRFLYQSTNPSSSTTFSXNNXNMNSNLGYPLADSTQVSTRQPSVSNTPNSTNSSSXSNTRNNTHSHNRLRSLPLINESELHSLQNQHSTATGATAVTTNHXKAQQNSANANQLVNTNDLNQVPLEELDFLKLSTDQYGCRFLQKKLEIPAVSDQFRDLIFFNTQDHFLELIIDPFGNYLIQKLCDYITTDQKTTLLKKIXKNLYQISINQYGTRSLQKIIDCICSTSNSTNGNAKDNASQQNNHQEVITKLINFMMMGFNPIYGNISIKQIIDLTNDLNGNHVVQKCIFKFPPLYYDFIIDSIIDNDNIVKVSTHKHGCCVLQKLLSSCTIQQIFKISIKILEYSPNLINDQFGNYILQFLFEIDDLNFYVLNEFYNKLQENLVQLSCLKFSSNVVEKFLKKLFKIINENSNMNSETSFNSFEPTNGTRSKSNSVTSSICTNKSLADSSRKSSRGQLIPHVEEDDVLQVAQQIMLNILDLFVLNLNILIRDNYGNYALQTLLDVKNYSQFLCLDEVYKVDCSFYTQFSSKIKRLMLITKNLLPSIKTTSYAKKIKLKVKAYTELTGFSLDQPFADPVADISSNYDGYSNMSTSSSFSSFPDISKNYEKVRNQNVPHGLEAPSLSQVFNKAHTRHVSLPTNAFSPANENQFDTLGAHKQYYENNENSAMANTMMMNADNETVPSVMSVTPSVTSFAGPNLAHQRSTSAVSFNQPTQAAYTAFAQTQQPVMAQQNTHLDSLSIGPSLYQRGGKQDYGSNIWSTPSLQQSSSNYSVHMNGNYYGSNSSNFAPNSNTSSDAFFGNXH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB52</accession>
    <name>A0A1E5RB52_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">37S ribosomal protein, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2631</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RB52"/>
    <dbReference type="FunCoup" id="A0A1E5RB52">
        <property type="interactions" value="124"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB52"/>
    <dbReference type="InParanoid" id="A0A1E5RB52"/>
    <dbReference type="OrthoDB" id="5223508at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23703">
        <property type="entry name" value="mS26_PET12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058940">
        <property type="entry name" value="mS26_fungi"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26163">
        <property type="entry name" value="mS26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="313" mass="35555" checksum="BDEACA30BFFF94AC" modified="2017-01-18" version="1">MGKGTLRFGGKSGILPPARALLKNPTTKQKPVYLQNLEEESSKVKTGYADNIAHPKGTSRESRPVEFLDVEARISKTIPEPTLDRSSGSAVAKAKANMAALRREYLGQSLRNEESRLLKQQELLEKKKEYLKVKAEREQAALLKSISIDENLTTPTLHDMMTYLPKDGDKKRTPIMRQRTEEEKEVLLLKRQHNRELLEIQSKERKLEELLRLFHVSEDLIVTEKDLSHYLDDVFKTDASSMLTRHNTLQQISANEESEMMNGLFGVVSRSSNSQPYVGLAKTTEFVSGEMESFLTEVQQKIKDLPADNQNNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBC3</accession>
    <name>A0A1E5RBC3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">U6 snRNA-associated Sm-like protein LSm4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3044</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBC3"/>
    <dbReference type="STRING" id="56408.A0A1E5RBC3"/>
    <dbReference type="InParanoid" id="A0A1E5RBC3"/>
    <dbReference type="OrthoDB" id="747253at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071254">
        <property type="term" value="C:cytoplasmic U snRNP body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071208">
        <property type="term" value="F:histone pre-mRNA DCP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071209">
        <property type="term" value="F:U7 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01723">
        <property type="entry name" value="LSm4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034101">
        <property type="entry name" value="Lsm4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052840">
        <property type="entry name" value="U7_snRNA_Sm-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21196">
        <property type="entry name" value="U7 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21196:SF1">
        <property type="entry name" value="U7 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="1" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="3">
        <location>
            <begin position="2"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="83"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="106"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="118"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ84197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="187" mass="21687" checksum="37E3AAAF1A2BA7F3" modified="2017-01-18" version="1">MLPLYLLTNSKGQQLLVELKSNEKIQGILDKVDSWMNLTLKNVTLTQFGEEHEIPELYIKGRFIKSMTLQKDIIDTVKAATNTSKNSGINNNNNNNNNNNNDRYGRRSQHGHHHNYHNQHGNANYRRRNYNNNNSNNHYLNQSNYNGRQSRNNTGNNNQRQQQQTAGGYAEFHQPISQIEQSTHLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBK7</accession>
    <name>A0A1E5RBK7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">alpha,alpha-trehalase</fullName>
            <ecNumber evidence="5">3.2.1.28</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g3020</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha,alpha-trehalose + H2O = alpha-D-glucose + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:32675"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:17925"/>
            <dbReference type="EC" id="3.2.1.28"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyl hydrolase 65 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.28" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBK7">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBK7"/>
    <dbReference type="InParanoid" id="A0A1E5RBK7"/>
    <dbReference type="OrthoDB" id="200349at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004555">
        <property type="term" value="F:alpha,alpha-trehalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015976">
        <property type="term" value="P:carbon utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005993">
        <property type="term" value="P:trehalose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000032">
        <property type="entry name" value="Vacuolar acid trehalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.40">
        <property type="entry name" value="Glycoside hydrolase, family 65, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037018">
        <property type="entry name" value="GH65_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005195">
        <property type="entry name" value="Glyco_hydro_65_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005196">
        <property type="entry name" value="Glyco_hydro_65_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11051">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11051:SF8">
        <property type="entry name" value="PROTEIN-GLUCOSYLGALACTOSYLHYDROXYLYSINE GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03632">
        <property type="entry name" value="Glyco_hydro_65m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03636">
        <property type="entry name" value="Glyco_hydro_65N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0574">Periplasm</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 65 N-terminal" evidence="12">
        <location>
            <begin position="142"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 65 central catalytic" evidence="11">
        <location>
            <begin position="500"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ84276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1227" mass="142948" checksum="AFE6ED2D118333A0" modified="2017-01-18" version="1">MIADRLCDRFTNDPQKCKEVCRRIGRKFVLLILIIWVSMASMFWFQYIKIFDCKLMKXENDNDLAXTSMSSPLTDLSIDTSDYYNTFLKSSKKFDSKILLYRDKSQQWLNMLKQDHDFHYNPEQMTLLSEKFYARNEFKYQPYVANGYIGCKLSNLGFGFTYDQNIPEIENGWPLRNQRYTGAFISDFYSLQESLNSTNFPELDKLGYSSVISSIPYYNDFIIEFEDPITLDKKILKPSLLESLNPINFKNYSQILSMKNGIVKTSYIWYDVLKLEIECIANKQYYTMSTISLHIDVLDKSKIQNLKIINLLDFKTSQRTDLNDLGYNIDEKTIFMSVSPNNVAELSNAAVASKIMVSSNLILNKNYNIQYEVSHECNSIGNEDSKKTKTKTSNDGLKMNCVKQIISFMDNENNTNTHHGYNDNANHFKIYKFVSIHSTEYTNSMDNLKLSQRELSNYHSIKDFQYLKNVHINRWIDFYTMNCFIEIPSDILLELSIRSSIFHLISNIREGNILPNRGIPISPSGLSSDSYGGMVFWDADYWIMPAMVPFFPGSAKNMIIFRNATLPQAIENAEFYNQPGAVYPWTSGRYSNCTSTGPCMDYEYHVNXDIVWSMFFYYLSGNYNMEKDNFDDEVFLRYTLWPMVENVTIFFEHYVTYVPLKDYYVTYNLTDPDEFANFVDNGAYTNFGIKMLFVWAIDICKHLNLKIPKKWFDIAQKMYMPMNDSNDITLEYSTMNSTVRTKQADVLLNIYPFGIFHKFPNHTQMVNNLYYYAEHQAYDGPAMSYQIYVAVSNYLLNQGCSSQSYLYKSLLPYIRLPWVQFSEQADDLDPLTKPAFPFLTANGGFLQSIIYGLTGIRFNYEFDKKTKKIKKFMEFNPTPLKVLPGGITIRNFKYMNGILDILIDDEYAKIKYKFGTQPIVIKVPDRTKLDLLDGHHNSIEFFKAHTLRPNTELEIKLFDLDYNMEGNLAECKLITNLTEAVPGDVSISMIDGNNYTQWQPINQKRATFLVDIGSQNEQIISSCTILWGNRPANNISMYVLPMDEFNHDKIPFIFEPSDFSQYSAMVEDRNTFLDQKVNQLEVAIAMGKYTEQDLLQMFVPIVEDYVIEPSHPYSENDRSNDNIQIKANNETTFFFDYDCEKMPLGRVSHDNEERESKKKKYLKDFYSKQSDGKKICHEAEKYKTRFILVSIEGVYGVDHEYTEKEILLNDIPVFQTFGATIREIGFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBQ1</accession>
    <name>A0A1E5RBQ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="19">DNA repair protein RAD16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AWRI3579_g2806</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBQ1">
        <property type="interactions" value="321"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBQ1"/>
    <dbReference type="InParanoid" id="A0A1E5RBQ1"/>
    <dbReference type="OrthoDB" id="448448at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000109">
        <property type="term" value="C:nucleotide-excision repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16567">
        <property type="entry name" value="RING-HC_RAD16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000753">
        <property type="entry name" value="DNA repair protein RAD16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001864">
        <property type="entry name" value="DNA repair protein RAD16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF12">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="237"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="590"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="676"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="137"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OEJ84311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="843" mass="97068" checksum="AA3BBDBCCABA56BA" modified="2017-01-18" version="1">MSSSDESLGGGFVKNDPDLKKRRSTRLRKPQKRVVYTIESSDEENEEKADILPDGKLKKSTTRATRSKANPVKSKALSAKKLKPSKKIDDDDDDDDDDLEYVEIGNDNIVKVDSENSDGEGFEMAAHDPEFVEELTEEIKEIKNDEGDNKATSKSKRKKAPAKKKAPAKKKNGEKKPKIDYYQRTTTKLFEQHPRIKDFFPDLRDTPLSKPERAQQPLLMKTKLLPFQLEGLHWMKAQEAGIYRGGILADEMGMGKTIQTISLLMADLDEEKEKKRVTLVIAPTVALMQWKNEIETHTQGLLTSYVFHGPARNKVNKHKTQKELESVFNDYNVVLTTFAVLESCYRRDKYGVKRKHGLYKEKSLLHHFPFYRVVLDEAHNIKDRQSNTAKATTALVTEKKWCLSGTPLQNRIGEIYSLLRFLDIDPVACYFCTKCDCESKLWRFSDNMHCDECNHVIMQHTNFFNHFMLKNILKHGMEGPGRESFDNIQTLLKHIMLRRTKVERSDDLGLXPRIVKVRRDFFSPEEKDLYQSLYSDISREYNGYVEEGVVLNNYANIFTLITRMRQMADHPDLVLHKLRDANQSKDLIVCQLCDEVAEDPIESKCHHKFCRLCIQEYVESAIDEHPTCPVCHISLSIDLSQPPLLNENIEIDTKKKSIVSKIRLDGTWKSSTKIEALVEELYNVRSENKTIKTIVFSQFTSMLDLVEWRLKRAGFKTVKLSGNMSPTQRDETIKYFMNNINVEVFLVSLKAGGVALNLCEASHVVLLEPWWNPSVEWQSGDRVHRIGQHRPVEIVRFCIEDSIESRIIELQEKKANMINATINKDEAAASRLTQDDLQFLFNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RCE0</accession>
    <name>A0A1E5RCE0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">U3 small nucleolar RNA-associated protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2643</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UTP5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RCE0">
        <property type="interactions" value="837"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RCE0"/>
    <dbReference type="InParanoid" id="A0A1E5RCE0"/>
    <dbReference type="OrthoDB" id="30195at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007148">
        <property type="entry name" value="SSU_processome_Utp12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052414">
        <property type="entry name" value="U3_snoRNA-assoc_WDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44267">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44267:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04003">
        <property type="entry name" value="Utp12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small-subunit processome Utp12" evidence="6">
        <location>
            <begin position="451"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="629"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="557"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="629"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ84253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="684" mass="76994" checksum="B0192C210463EB67" modified="2017-01-18" version="1">MSSPVILSEFNSNGDVVGYVTVELNKQRLTCKNLTSNQILSTLYFDSASTINDQNALLQVSVLKFFEYNGVEICLVGLNNGELWLYSFNSNEVLNKLNTSSSFQINDVQVITLNNKQYLYCSDLNNTLYQFDLDTFELSKKWKLDEYLEDVVKFQVLNSQKVLLCSHTISLLDLQSLQVEMTFPGHVTQIQDIQMIPNTTYFITSAENDRFLNIYDYSNGTTKLVLNCSENISKLSQTGSELITCVTAAGELETFIKPFSNTDSTLTDSNTHTNKRKKRQQISKKSSIKLKFQQLSKPLKISACKNVSDHSVQILWLENVSVPFSQIIDINENIDYSSSNNTIVIAPKIVKTGSKHYQTSDLASNVSYKEGNALVTSGDNYTNIETTLSNVLNNLNEEELEVSLQDQLQLHSDKYGKSSSGTTTSHKKKKKDKKTVIGTLTVILQQALKSNDHSLLDQVLGGTLDEQVIKMTIYRLSPPLSVVLLERLAERIARNSKSFQFSSSSSSSSSSSSSSSSSSSILEVWCKWCLIIHGAYLTSVPNLMNSLSTLHSTMKKRASLLNKLNIIEERLDSVLQRNELYKKKERSNGHFVRANQEYDGYEYEEEMYNEEDGSAXAYEEDVEYVEELDDAGLVDDDGEEGEDEEEDDEDDDKGSDAAYLEQETGDFDIDKELVQEDAYSDEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDK5</accession>
    <name>A0A1E5RDK5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Xaa-Pro aminopeptidase</fullName>
            <ecNumber evidence="5">3.4.11.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g2306</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.</text>
            <dbReference type="EC" id="3.4.11.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the peptidase M24B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.9" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDK5">
        <property type="interactions" value="381"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDK5"/>
    <dbReference type="InParanoid" id="A0A1E5RDK5"/>
    <dbReference type="OrthoDB" id="9995434at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01085">
        <property type="entry name" value="APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.350.10:FF:000015">
        <property type="entry name" value="Xaa-Pro aminopeptidase app-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000007">
        <property type="entry name" value="Xaa-Pro aminopeptidase P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033740">
        <property type="entry name" value="Pept_M24B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032416">
        <property type="entry name" value="Peptidase_M24_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050422">
        <property type="entry name" value="X-Pro_aminopeptidase_P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43763">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43763:SF6">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16189">
        <property type="entry name" value="Creatinase_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16188">
        <property type="entry name" value="Peptidase_M24_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 17" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="9 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase N-terminal" evidence="15">
        <location>
            <begin position="89"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24" evidence="14">
        <location>
            <begin position="465"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24 C-terminal" evidence="16">
        <location>
            <begin position="687"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ84985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="749" mass="84923" checksum="A17B270F6BC699EB" modified="2017-01-18" version="1">MSSVGVNPVSAGFKPCADCTCSRGLLYRQRQRSVLFRQLSRRHSGFYKPSSEEELGHGRSNSVTSSDNHSISESIISECETRNVNTSQRLLALRQEMAKEDICCYIVPSEDEHNSEYVSFADQRRAFISGFTGSAGVAVITRDLLNFNLDSPEGKSMLSTDGRYFMQAAQELDFNWTLNRQNVDKKLWQEWCIDECIDMWKSLGADPKKICKIGVDPKLFPNYKKLEFEKMIAKYGAQDNVKLVPIEQNLIDTIWGKFEQEPVRXLKEVYLLEDTYSGESFESKRKNLITKLDEHCSSNSSKGKIGNHFFVISALDEICWLLNLRGSDIEYNPVFFSYVIINGEKTTLFVDNVLXEGVEKYLRDNNVQVEPYKNFWSYLEFGFELDQNKNTXDHLPSGFYVPLNCSWAVVNQLSKQLASLSSVVSSKSDKKSRPSLSYAITPISSPLSELKSIKNVTEQHNANRAQVQDATAIIQYFAWLENELVKKGSLIDEQKGAXKLLKIRQTLPNFKGLSFETISSTGGNSSIIHYSPPVENSSMIDPSKMYLCDSGAQFLEGTTDITRTVHFTKPTKEEIANYTLVLKGNLALERLKFPCNSTNGYQLDVLARQFLWQYGLDYGHGTGHGVGSFLNVHEGPIGIGNTNRALLDHVLQEGQIITNEPGYYKDGEYGIRIENDLLVVKSPEVPGFLQFENLTLVPYCKKLINPKLLTSEEKNQINKYHSKIWTILCQYIQPQSITFKWLKRECSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDK6</accession>
    <name>A0A1E5RDK6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RDK6"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="88"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="165" mass="18329" checksum="44F0CC1BBE7B5F9D" modified="2017-01-18" version="1">MSTVEPTNTEQSFHKNDTNTXXQQMQGSPLKNETSAKPSSPMKNEDSAKQDDKSTVNKKPQKWILSQDTLKIKSFTGYTLSLPQWQPTSTSSTSESXTSTTTEKXLNSPDENKSGESDKKTLSEINSTNISKNKDMLDETKIGDKRKTDYHADPNAKKIAVPPKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDT2</accession>
    <name>A0A1E5RDT2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">18S rRNA aminocarboxypropyltransferase</fullName>
            <ecNumber evidence="6">2.5.1.157</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">TSR3</name>
        <name evidence="10" type="ORF">AWRI3579_g1811</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Aminocarboxypropyltransferase that catalyzes the aminocarboxypropyl transfer on pseudouridine at position 1191 (Psi1191) in 18S rRNA. It constitutes the last step in biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N(1)-methylpseudouridine(1191) in yeast 18S rRNA + S-adenosyl-L-methionine = N(1)-methyl-N(3)-[(3S)-3-amino-3-carboxypropyl]pseudouridine(1191) in yeast 18S rRNA + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63300"/>
            <dbReference type="Rhea" id="RHEA-COMP:13852"/>
            <dbReference type="Rhea" id="RHEA-COMP:16309"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
            <dbReference type="ChEBI" id="CHEBI:146234"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>an N(1)-methylpseudouridine in rRNA + S-adenosyl-L-methionine = N(1)-methyl-N(3)-[(3S)-3-amino-3-carboxypropyl]pseudouridine in rRNA + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63296"/>
            <dbReference type="Rhea" id="RHEA-COMP:11634"/>
            <dbReference type="Rhea" id="RHEA-COMP:16310"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
            <dbReference type="ChEBI" id="CHEBI:146234"/>
            <dbReference type="EC" id="2.5.1.157"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TDD superfamily. TSR3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.157" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDT2">
        <property type="interactions" value="792"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDT2"/>
    <dbReference type="InParanoid" id="A0A1E5RDT2"/>
    <dbReference type="OrthoDB" id="10262062at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106388">
        <property type="term" value="F:rRNA small subunit aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01116">
        <property type="entry name" value="TSR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022968">
        <property type="entry name" value="Tsr3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007177">
        <property type="entry name" value="Tsr3_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002621">
        <property type="entry name" value="PRK02287.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426:SF0">
        <property type="entry name" value="18S RRNA AMINOCARBOXYPROPYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN TSR3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="Fer4_RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04034">
        <property type="entry name" value="Ribo_biogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNase L inhibitor RLI-like possible metal-binding" evidence="9">
        <location>
            <begin position="50"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="16S/18S rRNA aminocarboxypropyltransferase Tsr3 C-terminal" evidence="8">
        <location>
            <begin position="88"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="246"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="264"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="299"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="10">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="317" mass="35898" checksum="520311D2893BD384" modified="2017-01-18" version="1">MGKGKNKSHDSRSSHTNSRGSNGHSIRQNHKRMEXKHTSVDAEGKRRFPVKLAMWDFDHCDPKRCSGKKLERLGLIRSLKVGQKFQGIVVSPNGKGVVCPDDLAIVEECGAAVVECSWARLEEVPFSKIGGKHERLLPYLVAANQVNYGRPWRLNCVEALAACFAIVGRTDLAAELLSNFSWGLGFLELNKELLELYQQCTDSDSVKAAEEKWLQKIEDEQKERKNQSRDEDIWLAGNVNRKSKNEVFSDEDSEYSSSERDDDHDNADDDDDTSVTYDALGNIIEKKEPKYDSLGNLIEDFDELEVEDEEDEESEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE23</accession>
    <name>A0A1E5RE23_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Multiprotein-bridging factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2088</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Transcriptional coactivator that stimulates GCN4-dependent transcriptional activity by bridging the DNA-binding region of GCN4 and TBP (SPT15), thereby recruiting TBP to GCN4-bound promoters. Involved in induction of the ribosome quality control (RQC) pathway; a pathway that degrades nascent peptide chains during problematic translation. Required to prevent stalled ribosomes from frameshifting.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MBF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RE23"/>
    <dbReference type="FunCoup" id="A0A1E5RE23">
        <property type="interactions" value="762"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE23"/>
    <dbReference type="InParanoid" id="A0A1E5RE23"/>
    <dbReference type="OrthoDB" id="10253401at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000015">
        <property type="entry name" value="Endothelial differentiation-related factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013729">
        <property type="entry name" value="MBF1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10245:SF15">
        <property type="entry name" value="ENDOTHELIAL DIFFERENTIATION-RELATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10245">
        <property type="entry name" value="ENDOTHELIAL DIFFERENTIATION-RELATED FACTOR 1 MULTIPROTEIN BRIDGING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08523">
        <property type="entry name" value="MBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="8">
        <location>
            <begin position="85"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="150" mass="16283" checksum="2A9ED82E19076776" modified="2017-01-18" version="1">MSDWDTPTIIGRKARAGGSGPRSAIARTQGQVNAARRDGMVMSVDKKYSTANKKGDIEGQRLTKVDRDDDIVALKKIDLNVGKTMSQARQAKGISQKDLATRINEKPTVINDYEAGRGVPNQQVLGKIERVLGIKLRGKNIGAPLFGPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE57</accession>
    <name>A0A1E5RE57_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1876</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RE57">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RE57"/>
    <dbReference type="OrthoDB" id="4024574at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="unsure residue" description="D or N" evidence="1">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ85164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="494" mass="57603" checksum="281160E9EF6218D1" modified="2017-01-18" version="1">MVMKKVNVIYCEAITFALVLLMISQVGIGMPITTPFNQYIQAQDKICTSSYVDDQISYRKIVAKLFGLPGNGMFSSLKPPKEYTGFKLIKKHYIKNAFTGILSHISFNYCEHGKLLWNKDFPISLAQLYDFDWDKPICVYYRSSLQDKIVSSSFDINFTNATNVKKHNETKQLKTMLAQTRKSVKGPLKFQSMEKQELPSLKSLALNDPENFFCFKYARRKKYANSDLNVFFPQQWVGGLFYCDLNVEEKVNFLMSNMDSDLNXINQNLVHNTCSFANAIPMLPVIADQYNNYWTDYEGFKTTTKKRNKKEAHFEMVKNVPYLYTPQMTNIFDLKTKGTLEYNETSNIDEYHFQKKMPPSFLPNWKFKALDYFRKTPFMAQAEKWFNDISVTESYKYNQSDMNGLEYFAQLTLTKLQKMAKAETFDESAVWSMNEDHLSKNKMLKLHKFAISKYLTLEDFLVGLISSKLFKQDSEELQLAAESLQVDDQKNFDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE91</accession>
    <name>A0A1E5RE91_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Serine/threonine-protein phosphatase 1 regulatory subunit GAC1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1996</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE91"/>
    <dbReference type="InParanoid" id="A0A1E5RE91"/>
    <dbReference type="OrthoDB" id="1881at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000164">
        <property type="term" value="C:protein phosphatase type 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001069">
        <property type="term" value="F:glycogen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005979">
        <property type="term" value="P:regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2440">
        <property type="entry name" value="Carbohydrate binding type-21 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005036">
        <property type="entry name" value="CBM21_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038175">
        <property type="entry name" value="CBM21_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050782">
        <property type="entry name" value="PP1_regulatory_subunit_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307:SF51">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT GAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03370">
        <property type="entry name" value="CBM_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51159">
        <property type="entry name" value="CBM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM21" evidence="2">
        <location>
            <begin position="811"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="494"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="605"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="741"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="963"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1107"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1256"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="159"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="495"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="509"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="535"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="555"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="612"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="977"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1026"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1110"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="1039"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1275" mass="142857" checksum="323807103410B8C6" modified="2017-01-18" version="1">MSNFYRNDNANNSNETIIISNDTENGGYEHDYNYNEKEXFDDMNEFTQNIDNNTRKTEKDQVNQVGQNYQNDQNDQDSLMESDHEDYKGLNGLYLKNLNGILDHIEKPRTDTSKESKKSNEKDINDNTLKRNDSDNSISTIISQNNENPNDSKNQLKITTTTPNETLTNNTQPQLENINKDTEKQVSPSLSIFVTAPLNTLLETMSNSTTSTSNSTISSVASDVGPLDANINGTTNVINNIIPNLNNPPFNFTQNFNNCSFPSKIXSSNTWNHFKNINFNNXNNTSIDPPVTAASNTSINTINTSNSNTPLQPRIMKSSLKNSSGNLIVYGKESVTADSYRNSISTTNTNSLADTTSSTMSNNHHLFPYSPSISSNSYTNLKSRSASITSLSSSTTSQKSVRFSDKLVNVKTFRSNDEPAVVAKADXKYAKNKLLGSAGQLSRMISGTSSPSPLNFEVLEKNSNASSSNSNSYLLFDEFDYDDELDDLNELKYRNNGSMKKNGNRNNSDEDEDEDEYEDEYEYEDDDNEDSSYNRSPINQPSNRRYSKFSSNYKNNTSNFATGNSSTFTSNNNNNTKKISNKNNSSTETLKSQRFLSLLNEYSDDYDKDENTYSTKKNTTNTLNSDKSNNNGYSNGYTEGNYRDIISKKHLINSFNLLSQYDTSHDLANSKGSLSKSCAEEQLKCSSANPLTNVNXKLNSNLNLPNGFDKYTDLENYKTNKNHFSSKNEPLNQKLALSKFRSSIGGNSGNKRRTSNLHQNNKRSSTSLSSLSSASSTSIQKLAEPKIIQEPSTWELSNPNFTNIYSFFGNENAVDHQNVKLQSIQIDKLSGLVKGKLHVLNLHFEKNLTIKYTLDQWTNMHYSQANYVKTVNSKIDEFGFEIDLAKLNETNQAIFTLTTSQTNKKPFVKLDSVPSLSLASKIKNIKLYKMELCCIYNVNDQTYYDNNNYQNYKFEIKEFIQNSKKNQQSREKAKPHTNLNRSWSTNDTTSLSYHLKNSNVSNSKASPEGSPSKRSNLVHPRISSILDDSSMVTSNKNDDSSSQVKNQRPGLTQRSFSNDTEYFNNNPMKHLYHKTNVSTSETNLPSLEAGTTLFNVKNQELLNYKLENMSRSPSPSPSQSDSTPKMFTNNLNYGDXSGINMSTIGCAKSDVALNLQNPLLSVENNHRPPSAPLLGSALKYDNILDASSSKKDLEIDEALLTQIGASSSKKATFTMXDTXQDPKISEKGNNSHSTDKKQTNYNDLLERFCFFVPDSPSAPSTPLGSRTEIRNMSYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REE2</accession>
    <name>A0A1E5REE2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA topoisomerase I</fullName>
            <ecNumber evidence="7">5.6.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">DNA topoisomerase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1825</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6 7">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">Belongs to the type IB topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REE2">
        <property type="interactions" value="797"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REE2"/>
    <dbReference type="InParanoid" id="A0A1E5REE2"/>
    <dbReference type="OrthoDB" id="47179at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00659">
        <property type="entry name" value="Topo_IB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03488">
        <property type="entry name" value="Topoisomer_IB_N_htopoI_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.41:FF:000001">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.10:FF:000003">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.11.10:FF:000001">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.15.10:FF:000002">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.11.10">
        <property type="entry name" value="DNA Topoisomerase I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.15.10">
        <property type="entry name" value="Topoisomerase I, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.41">
        <property type="entry name" value="Yeast DNA topoisomerase - domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013034">
        <property type="entry name" value="DNA_topo_DNA_db_N_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013030">
        <property type="entry name" value="DNA_topo_DNA_db_N_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001631">
        <property type="entry name" value="TopoI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025834">
        <property type="entry name" value="TopoI_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014711">
        <property type="entry name" value="TopoI_cat_a-hlx-sub_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014727">
        <property type="entry name" value="TopoI_cat_a/b-sub_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013500">
        <property type="entry name" value="TopoI_cat_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008336">
        <property type="entry name" value="TopoI_DNA-bd_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036202">
        <property type="entry name" value="TopoI_DNA-bd_euk_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013499">
        <property type="entry name" value="TopoI_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018521">
        <property type="entry name" value="TopoIB_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048045">
        <property type="entry name" value="Topoisomer_I_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051062">
        <property type="entry name" value="Topoisomerase_IB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10290:SF3">
        <property type="entry name" value="DNA TOPOISOMERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10290">
        <property type="entry name" value="DNA TOPOISOMERASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14370">
        <property type="entry name" value="Topo_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01028">
        <property type="entry name" value="Topoisom_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02919">
        <property type="entry name" value="Topoisom_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00416">
        <property type="entry name" value="EUTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00435">
        <property type="entry name" value="TOPEUc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56741">
        <property type="entry name" value="Eukaryotic DNA topoisomerase I, N-terminal DNA-binding fragment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00176">
        <property type="entry name" value="TOPO_IB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52038">
        <property type="entry name" value="TOPO_IB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="DNA topoisomerase I eukaryotic-type" evidence="10">
        <location>
            <begin position="326"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="658"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="92"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="active site" description="O-(3'-phospho-DNA)-tyrosine intermediate" evidence="6">
        <location>
            <position position="765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="807" mass="93794" checksum="D293AA82ADE2E258" modified="2017-01-18" version="1">MATASTRPKRAAVNNSIIEPDSDYDDFKSNKSNKSSNSKKKTLKTSEKIDFKSFDDDDDDDDEDDKIVKAPAKKKRKLSSSTTKKPTTKKIKKEENGTAKGDKQKKAAIKKITKNSKTPAAKKKTVKQEKKDEKIKSIVKNEETEDAVKAAKEEEDFEKAEEYKWWEQQNEDDTIKWQTLVHNGVIFPPEYEPLPSHIKLYYXKKPVTLPPQAEEVAGFFAALIESEHAKNPVFQKNFFADFKQVLKESGGVVDPPKFNIKDFSKCDFTKIFEYFELKKQEKKQLTKAEKKQIKLEKEKLEEPYKFCYLDGRKEQVGNFRIEPPSLFRGRGAHPKTGKLKRRVYPEEVVLNLGADAPVPEPPAGHHWGEVKHDNTVQWLAMWRENISNSFKYVRFAANSSLKGMSDFKKFEKARELKDHIEVIRKDYEKNLYSKVTFERQIAVATYLIDIFALRAGGEKSEDEADTVGCCSLRYEHVTLKPPQTVIFDFLGKDSIRFHQEVKVDKQVFKSLAIFKKHPKKPGDQLFDRLDPSILNKHLQNYMPGLTAKVFRTYNASKTMQDQLDLIPNTGTVAEKILKYNAANRTVAILCNHQRTVGKNHEQSVLRATEKIEEWEWSKIRLKRAILQLDPEQAKVKGQVRYFSEIKKLTKTKEKKIHKRVLLKEKEKLEKKFQRENEKLKENKEPVLKASVLKEWLAKIDDLEKQYEQELENDGKWDEELLKKQTVTKLKAQVEKLEARIRTNTIQLKDKEDNSQVALGTSKINYIDPRLSVVFCKKYNVPLEKVFTKTLRDKFKWAVESVDSDWRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REF1</accession>
    <name>A0A1E5REF1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">3'(2'),5'-bisphosphate nucleotidase</fullName>
            <ecNumber evidence="3 11">3.1.3.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1965</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'-phosphate (PAP) to AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>3'-phosphoadenylyl sulfate + H2O = adenosine 5'-phosphosulfate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77639"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:77640"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>adenosine 2',5'-bisphosphate + H2O = AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77643"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:194156"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:77644"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>adenosine 3',5'-bisphosphate + H2O = AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:10041"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.7" evidence="3 11"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REF1"/>
    <dbReference type="FunCoup" id="A0A1E5REF1">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REF1"/>
    <dbReference type="InParanoid" id="A0A1E5REF1"/>
    <dbReference type="OrthoDB" id="411145at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008441">
        <property type="term" value="F:3'(2'),5'-bisphosphate nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043647">
        <property type="term" value="P:inositol phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01517">
        <property type="entry name" value="PAP_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006239">
        <property type="entry name" value="DPNP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051090">
        <property type="entry name" value="Inositol_monoP_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01330">
        <property type="entry name" value="bisphos_HAL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43200:SF6">
        <property type="entry name" value="3'(2'),5'-BISPHOSPHATE NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43200">
        <property type="entry name" value="PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ85282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="373" mass="40977" checksum="3853CCB602E190A8" modified="2017-01-18" version="1">MIYNKEVRVAVNAVRRASILTAKIQKQIIANRSSTTITKSDESPVTIADYAAQAIVLNAIKANFPQDEIVAEESTEGLSESFLNNILSTINEYAPKEDDETLFNEDIPLSNSEYPLKTTNDVQNIINLGNSKGGAKTRFWCLDPIDGTKGFLRGEQFAVCLALIEDGEVKLGCIGCPNLKLNDYVTKDNSLGVANPLNRSFHDPAEFGYIFYAIAEEDHLSYLHTTDLLSGSLKSVAANEFTVSASENLVSLEGVEKGHSSHDNQQIIKDHFHINQSLHLDSQVKYCLLVLGLGHVYLRLPTSMTFEEKIWDHAAGNALIRAVRGNHTSSLSAEPLDFSQGRTLKTKGVIASIATPELHKDIVSFAESVINRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REH8</accession>
    <name>A0A1E5REH8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Inorganic phosphate transport protein PHO88</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REH8"/>
    <dbReference type="FunCoup" id="A0A1E5REH8">
        <property type="interactions" value="261"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5REH8"/>
    <dbReference type="OrthoDB" id="18139at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012098">
        <property type="entry name" value="SND3_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28112">
        <property type="entry name" value="SRP-INDEPENDENT TARGETING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28112:SF1">
        <property type="entry name" value="SRP-INDEPENDENT TARGETING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10032">
        <property type="entry name" value="Pho88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008756">
        <property type="entry name" value="P_tr_PHO88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="180" mass="20355" checksum="53239654F96EF0E8" modified="2017-01-18" version="1">MNPAVTNIGLMLLMNQFAKRLDMENKDTIFYIRVAFVAMNLITYLVYQYTRMQIIKRNDLTTLKYKEQKSAFQSMSDPNAATDDMHITTHRDYDLGQVDTAIKGMYSSLAMMGFMHLYMGYTQPLLMQSVSPVKGAFESPAVKIHLFGKEAAGALKRPFTAPSMFGGAPKNEEVEAKKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REJ4</accession>
    <name>A0A1E5REJ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ATPase expression protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2438</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REJ4">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REJ4"/>
    <dbReference type="InParanoid" id="A0A1E5REJ4"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ85315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="684" mass="79131" checksum="9A62176BFA042078" modified="2017-01-18" version="1">MSNVQQLSLGKLLPNLIPVRTQQLPKTRIITLNSFPKHEPIPLKYKKDHLKSKAKTVNFYLNHKNEKLRAGKGSNYGLKIATELQSTAESDDFLEDEMSERKERIAQGTLSRQKKVKIAELATIEKTICPSDGGYQRDSLAALRTLLDQEKSQFEKKLAETAQYYRSLQKTAKGQIFLYNNFQEKVKSMLSVLQAITNPHRLEYPPEEMKIYPTEVTTEIPPLAVSSMDQRQFNDYIGTLTHTKFKFKSSSKSSGIVPKILRLLMNKDNFTTQHLRNTEAYNDTIFFFKKKGDLSTCLEYFEQMERESIKRNTKTYNLILRLISDIQLKGLYTMPAIERFLPLLKTMKTKKVYADIHTWGLAYSILFDNHSKQHFLQTMKECKLPASKNLITHLIKENYQKNDVFQIIDFLHNTNAPFNSAAINLVLKKLIHENDFQGYFLVLNFAKFVGSSMSLAQFTPNSMNIYLAKSADLGRVDLAIMGLHTLMRDFHIQPDSKTFELLYKTLARRGVHKLYHYGFDYVDNLCYRLNQNNVVHCYWRTKALSILYVDCQSKKKLLEKGAILSTTTRNEMHQKLRILNSGIGFETEVWNAGHATLFRFFRILGSVPSKYYNTYKEXLKIKAIGCTAAASSNSNKRHKYMQGIRNIGLHRELSNRVKYLENGVYEGLKSDILERDLLIMKEQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REM5</accession>
    <name>A0A1E5REM5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">rRNA adenine N(6)-methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1789</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>adenosine(1779)/adenosine(1780) in 18S rRNA + 4 S-adenosyl-L-methionine = N(6)-dimethyladenosine(1779)/N(6)-dimethyladenosine(1780) in 18S rRNA + 4 S-adenosyl-L-homocysteine + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42780"/>
            <dbReference type="Rhea" id="RHEA-COMP:10234"/>
            <dbReference type="Rhea" id="RHEA-COMP:10236"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74493"/>
            <dbReference type="EC" id="2.1.1.183"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8 9 10">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="10"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REM5"/>
    <dbReference type="FunCoup" id="A0A1E5REM5">
        <property type="interactions" value="806"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REM5"/>
    <dbReference type="InParanoid" id="A0A1E5REM5"/>
    <dbReference type="OrthoDB" id="74991at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052909">
        <property type="term" value="F:18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000007">
        <property type="entry name" value="rRNA adenine N(6)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011530">
        <property type="entry name" value="rRNA_adenine_dimethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00755">
        <property type="entry name" value="ksgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727:SF7">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 10" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 9 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 9 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA adenine methylase transferase N-terminal" evidence="11">
        <location>
            <begin position="44"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ85013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="331" mass="37638" checksum="61687A5A5504CF7C" modified="2017-01-18" version="1">MGKADKKQKYTTATGKQVDAEKHLSTVFKFNTNLGQHILKNPLVAQGIVDKAQLKPSDTVLEIGPGTGNLTMRILEAGVKKVIAIEFDPRMAAELTKRVRGTPYERNLEIILGDFIKIDPLPYFDVCISNTPYQISSPLTFKLLNCQPPPRTSILMFQREFALRLIAKPGDPLYCRLSCNIQMYGDVQHIMKVGKNNFRPPPKVESSVVRLVPKLPRPTIKFEEYDGLLRILFNRKNKTLNSNFKNSSVLELLEKNYKIWKSQQQMGNGGEMVEDEEMEVDEGKGKNKEIKDLVDKVLQDLDMGDKRPQKLDQVDFLKILFAFNKVGIHFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REX7</accession>
    <name>A0A1E5REX7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Lactoylglutathione lyase</fullName>
            <ecNumber evidence="3 9">4.4.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glyoxalase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1734</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(R)-S-lactoylglutathione = methylglyoxal + glutathione</text>
            <dbReference type="Rhea" id="RHEA:19069"/>
            <dbReference type="ChEBI" id="CHEBI:17158"/>
            <dbReference type="ChEBI" id="CHEBI:57474"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="EC" id="4.4.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit. In the homodimer, two zinc ions are bound between subunits.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glyoxalase I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.5" evidence="3 9"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REX7">
        <property type="interactions" value="225"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REX7"/>
    <dbReference type="InParanoid" id="A0A1E5REX7"/>
    <dbReference type="OrthoDB" id="16820at2759"/>
    <dbReference type="UniPathway" id="UPA00619">
        <property type="reaction ID" value="UER00675"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004462">
        <property type="term" value="F:lactoylglutathione lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07233">
        <property type="entry name" value="GlxI_Zn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004361">
        <property type="entry name" value="Glyoxalase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018146">
        <property type="entry name" value="Glyoxalase_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00068">
        <property type="entry name" value="glyox_I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10374:SF30">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10374">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE GLYOXALASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00934">
        <property type="entry name" value="GLYOXALASE_I_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00935">
        <property type="entry name" value="GLYOXALASE_I_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="VOC" evidence="10">
        <location>
            <begin position="28"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="VOC" evidence="10">
        <location>
            <begin position="192"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="330"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR604361-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR604361-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="338" mass="39438" checksum="5497D2ECF67ED92D" modified="2017-01-18" version="1">MVETVTTPSNDIYTETITQIRLSKQHPTFNHTCIRVKDPVRSIXFYTEVLGMKLLSHKKFPDMKFDLYFLAFDNTPNVIKNESTVFYCSGVLELTHNYGTESDPNFQVNNGNVEPYRGFGHVCFTCFDIEKLSSLLESQKVEFKKRLVDGRQKDIAFIYDPDHYWIELVCYGNRPNYIQEGEELITEDHGLKFNHTMLRVKDPKKSIEFYTNVFGMTLLESKSHENAKFTVYFLGYLDKREIGPRRMREGVLELTHNWGTEEDPDFHYHNGNDKPQGYGHICVSLEDPGAFCKEITDKYSDEQIKWGVKFNQGKMKNLAFIKDPDNYSIEIVPHYLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF07</accession>
    <name>A0A1E5RF07_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2259</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FNT transporter (TC 1.A.16) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF07"/>
    <dbReference type="InParanoid" id="A0A1E5RF07"/>
    <dbReference type="OrthoDB" id="4829at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015513">
        <property type="term" value="F:high-affinity secondary active nitrite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015707">
        <property type="term" value="P:nitrite transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000292">
        <property type="entry name" value="For/NO2_transpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30520">
        <property type="entry name" value="FORMATE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30520:SF6">
        <property type="entry name" value="FORMATE_NITRATE FAMILY TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01226">
        <property type="entry name" value="Form_Nir_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="366"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="432" mass="48975" checksum="113F6991511F0012" modified="2017-01-18" version="1">MSDEDAVHFATNYSPDVAVTHFKTDTEVSPLEQTVKLSTRKIXENPDIKVTFIEEKVPPRKSKKKKLVKPARKLFGKKNAHKNXFIMVHVHDEDFSTPYEATLSCIQTGLKKARMKYYDMLITGFTAGLVFGIGGLLCTQVNGLFWANPRERDDNIGFVRFGRGMVSSLGLFFVVVLGIELFNSNVMFLTVAWCVGKVTIFDMVINWSIVAIGNIAATLFSSYVILFLIQRKGQTDLVGLGSQYFMNSRLGHNGVQHFIRGCAANFLVCQSVYFGIMCKPLHAKLLMIFIGVFTFATVGFNHIITELFLIPFSIFNVGAFEFDKVELYDFGQSAPLYADLLQNGQYREAQKKLVIRFITKMFIPSFFGNIVGGIVFTIIIPFYMHYYAVKQDAVKLGFKNFDYEDSRPDIISDSMMSKAKRIFXKARKNXKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF84</accession>
    <name>A0A1E5RF84_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g2450</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF84"/>
    <dbReference type="InParanoid" id="A0A1E5RF84"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ85570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="437" mass="50614" checksum="9B9DF300CB185E76" modified="2017-01-18" version="1">MRVIDNGSDIVHIDNAKNEKTNDDTLLQQILDIFNRYGKFKNDVSFDCIIKDEELLFNTRMEDVLKKLSILDTSFEQNFLLQNCNQCFHDYNILLNEKINFLINTWDLPIFNALVGNLDISIISNIISTNNTIKEKLKFHEKICISIFNKCNGILKDIIDPLIAKITDLAHFIIEYITSNGIQSLYLENELLLLFKLWSLTYLELLRNIEYYYIMIMKSRLLIILYELLKFKRKILYIQTEKYKEFKMGENYDKGIGKMLYGNHKNINNHLELIEVLKKYCNMDGVIIGYGKFIIGFLNAEANMSEANMSEASNSVSSTSGVNTNTINNKQTPTTDLTFNDCVDLFNDIEKMYLKLKSEMDMDFQEENDSMIFNKNRLENTSQLPNMQALFNTNKTVLDELPNLMNEFGVSKTGGKENVNISKTESVLDQVDRLGVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFK2</accession>
    <name>A0A1E5RFK2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Nucleoporin NIC96</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2329</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Nucleoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the nucleoporin interacting component (NIC) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFK2">
        <property type="interactions" value="1157"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFK2"/>
    <dbReference type="InParanoid" id="A0A1E5RFK2"/>
    <dbReference type="OrthoDB" id="1918363at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025574">
        <property type="entry name" value="Nucleoporin_FG_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007231">
        <property type="entry name" value="Nucleoporin_int_Nup93/Nic96"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11225:SF4">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11225">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP93 NUCLEOPORIN NUP93 DEAD EYE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04097">
        <property type="entry name" value="Nic96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13634">
        <property type="entry name" value="Nucleoporin_FG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="93"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="107"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1044" mass="117665" checksum="6F9C8918FF7EFEE0" modified="2017-01-18" version="1">MFKFGSTSNGASAPALGSTTQQPQXTAPQSTLFGNKSTTAGQSSFSFGDKKPTPAPSTFSFGDKNPTPAPTTSLFGNKQSTPAPANSLFGNTQQQQQQQPQQAQPQITNPTASTSFFGNTQQAQSTNPLNTASTQQITLNKPVIEVQKDLSKNKKRTALNDLVEASKNLPSLRDSADIPNVIGTIDLLGDSVQLSLDELSRRAKELFSKTQAAKDLTRAHYLLAGSGLPIQDAESTLENLNKVCRSRDLYHYKSRHHHHLDKSRSRFPVSSTDLDLYLKTKKDENILLSIEQSLNSAAKDFDAFVSSNFTLESWDKRKQEVRESFGILMKKRKLNNGXSGMAKDVSSGFTSTSSLSSTPWNTQNKGFLTKIMDNSRLNVNENVLIREKFENYAKIIHKFNNYRQNQNNELIGNFHLCQEIIDNVLLSEDAINESNNSAGVNTIVERFNILQSVSKNKNTTENSKHYLEXQFLSYVDELYLKNLRNEGLPSTLNKVQAFVQYKLKSSTTNQWKNKNLTIVNNIPIWALIFYLLRAGCPNEALQVAVENKNSFKKVESSFLTYLKHYCTTKNNALPLEFQSKIHTEYNQHLKNAVDGDPYRLAVYKIIGKCDLSKKVVNEVVLSVEDWMWFHLSLITSSDNGIGSSDSLEHYSLADFQATVTAYGVSHFGANNYIQVLLLSGLYNSVVEYVLGDVKVSNELDSFHLIFCLHIYDLLNLNQLQKFVTLDKIFATYISSFVNTDPKIAVEYILLLPIHGNTDLIEICHTMIRELVLNTKEFSVLLGRINSVDGTRIPGVLEERYRLMYIDDKXQFLHTITERAARKADEDGRIDDSLLLYQLSEEYDIVFSIVNKSLSDLLSSFDYLQKKQLQDILWDETDDTNVIVLASRLANLYLKQNNNLEISSKIGSKKKEALLVLLKIVDVWRLYTNDDYLACADMIKSLDLLPIFMSENVARRGIANGSTISNDTNEVVSKNIPNLLILTMSCISHLIKQTKQSAYQTEDQRKNSIAPLKKIAKNCMTYVGMTYYKMSRETYSILLNMESEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFP2</accession>
    <name>A0A1E5RFP2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Homeobox protein CUP9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1947</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFP2"/>
    <dbReference type="STRING" id="56408.A0A1E5RFP2"/>
    <dbReference type="InParanoid" id="A0A1E5RFP2"/>
    <dbReference type="OrthoDB" id="10056939at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="6">
        <location>
            <begin position="250"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="252"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="188"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="235"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="387"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="126"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="212"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="235"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="248"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="400"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="438" mass="49295" checksum="49062A58D93B57F7" modified="2017-01-18" version="1">MNVNNYTIDDKPNVNQNDIYQYHQPNITTNGVNNTMNGVNNTMNGVNNTMNGVNNTMNKTNNTMSEETCHYKYNYNYEYSYTSNNTNKDNILGGNILPQSMTRLPSIQYLLRAVEQTGINDVGANEPSSYDDSTNRMSSNKIPDYSIGSSLEVNPYHIKKNTYKPNSISSILYTSNETTREVNTSKVNMSEASTSGVNGYTSAADSNTTADNTSSITPYNTSNITSNITSNITSNITSNSAFTNSPAKHMKKRKRRSNLPKAVVSKLNQWLAQHMNNPYPTASEKLQLMNDTGLNSVQLSNWFINVRRRKVFNEYYTLAAQAPNHPPHKSIDENCVSVRAARKEQRKREKLETREKLNYVVEKMGDKNGFLSKVGLGALSEVNGKNGDCVGVEDDDDDNKPEADEKDLQERFVMAPLTRRKKLIDRLEELKNMTNSRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFQ6</accession>
    <name>A0A1E5RFQ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA repair protein REV1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Reversionless protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AWRI3579_g2205</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents. Involved in mitochondrial DNA mutagenesis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA polymerase type-Y family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFQ6">
        <property type="interactions" value="872"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFQ6"/>
    <dbReference type="InParanoid" id="A0A1E5RFQ6"/>
    <dbReference type="OrthoDB" id="427711at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017125">
        <property type="term" value="F:deoxycytidyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17719">
        <property type="entry name" value="BRCT_Rev1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.100:FF:000001">
        <property type="entry name" value="DNA repair protein REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000011">
        <property type="entry name" value="DNA repair protein REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.100">
        <property type="entry name" value="DNA polymerase, Y-family, little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1280">
        <property type="entry name" value="DNA repair protein Rev1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036775">
        <property type="entry name" value="DNA_pol_Y-fam_lit_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017961">
        <property type="entry name" value="DNA_pol_Y-fam_little_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038401">
        <property type="entry name" value="Rev1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45990">
        <property type="entry name" value="DNA REPAIR PROTEIN REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45990:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00817">
        <property type="entry name" value="IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11799">
        <property type="entry name" value="IMS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100879">
        <property type="entry name" value="Lesion bypass DNA polymerase (Y-family), little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50173">
        <property type="entry name" value="UMUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BRCT" evidence="17">
        <location>
            <begin position="190"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="UmuC" evidence="18">
        <location>
            <begin position="373"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="102"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="890"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="104"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00081902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OEJ85725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1030" mass="118202" checksum="F77C15BEB49EDD90" modified="2017-01-18" version="1">MNALTKEELKLLLESFDSNKSKNNNYNEGLNHEIFDTSELSPVLKSLKNSQSVYDPNVLLSDVESELLNKSDDIHFXDNNNLEXPNKEKSLNNVENYFXSTMDXKQEVETREEQEVETREEQEMETKEDQDKNKNENEDVSPFGSNNFLSLSDKITQSPTKIPPNTKLFSTESLDSSPAKKGRTEKRVKQNFKLFASINIYVNGYTDPGRLQLHRLMTLYGGKFTERLTSKKAVSHIIATNLTPKKKIEFAKYKVVRPQWITESIKAQKVLNWQEYSLFYETKTNITKNFAKVGPESGNEFPLVHTIDCNHPDFLASFYSKSRLHFLSTTKMLLQSKYLKYTLPPLSSTSHKTSFTPRGKFNPKKDFTPLKTIYYIDFDCFFAKVSSLMDPSVDIDKDCIVVSHGQNTADIASCNYVARKKYGIHNGEWVSSAKLKCGNDLKILPYHFAKYREISEYLQLYLKNKFDCIIPLSCDECIGYDYGELDYEKSQLKCESIREAVEKITKCTVSIGVSTTLFVSKLALKYAKPNGYKIFDPERDDVSEFISFFNLTDLPGIGYNIVEKINQLAXSYNKDQLAKDYKIYTIADLNHFMETQRKNSQLSLKESSFQLLAPIGEKLSKKLFNYLIEYKDDSESLQKLDQPLKYFXRKSISVDINYGVRFQNIDQVYKFLERICNYLLTQLQEISMCTNQLVLKLAKRLSGEPIEPSKHMGMGKCEFMSKLKNLRKYTAEKFIILPELKSLFHQLYKAYAISDIRGISVQFMRLSKTDVAIPKTSLLDFGMFKLKKLADNDAQLLDSAGTRTGKTRKCXSEPAAFPVTPQIGSSRRSTSPVKDFFENNSRKKSKLNIPDHIDMSVLRELPVNLQKELLAEYHLINTINTSRAKKILEKSTGKQGLSPKKSXCSGTTDSLGNVLEXAKFPQKNTPKRRVTFQTKQRPKEIRQLLQAWVHYSIDSGPLDEDKVLFVRFCQKLLSQNKPSNLILYITFLQNLVDYYKDKSSQDSSISTGVDAWSKYLLLQLQPLLSQCDPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFU9</accession>
    <name>A0A1E5RFU9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcriptional regulatory protein DEP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2252</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFU9"/>
    <dbReference type="InParanoid" id="A0A1E5RFU9"/>
    <dbReference type="OrthoDB" id="20886at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013907">
        <property type="entry name" value="Sds3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21964">
        <property type="entry name" value="BREAST CANCER METASTASIS-SUPPRESSOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08598">
        <property type="entry name" value="Sds3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01401">
        <property type="entry name" value="Sds3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="99"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="846"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="20"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="116"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ85463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="884" mass="98000" checksum="AFD53B4F1FCC0073" modified="2017-01-18" version="1">MSQGPGXLAAVASSNETSENKNINLNNDENKADNNSNNELQNNNNNNNNNTQTSLNTNPDGSTLAALGNTTRSNSLIWKTGLSNRSGSSKDYLSDNESELSYVGLSPEKDKYSLPFEEEEEEEDDDDADDSNGEGQQPEKEFLLKKSVTNAGKHNGVGVGVEGVGEDQKQTKGEIEGNGLARNAIAKEVLDAAIDEIGSDCETEKGAIVEEYERKEKEEVIRESSVVDDAQVVQREDFKEPNPNNQLFENDKSNTHQQQIQDNKHIQNEDLKGLPATTENNTNNLKRKFEQVGDTNEGTESNNKQGKRVQTPVMQINEKLSSETKDEVLDEVRSIEPPXDSILTSATDVAVVSPAPTNEQETNAVPLPIPSEXTVTIEDPIAADAVLKRVEEQTANDITQEILANKQEELSTLNEPPVNDESKLETKSLATVDNESKLETRSLSTVSNASMSITNDDKTQDATVELEVPLPDMSAADAEKTFENAELTMGNEVNGEFANEVQPAIKEGFGISVDQESQRLKGYEAICEIETDFANLRQEIYENKIQKLKMELQMCLDGSHSALQKYLAQVQEIRDEKLKKIYLKQQYRLDSIDRETRATRYILHQDFYKKTSDLKEALLNETTKQWYDINNERRDIDLMSNVSAAAAAAAHKDAHGANDLDDVEEERINLMNMLYSGNTSILHQENKYHVPIKILNKSLSMITGYSTQPALEIDQRTPHTYDTTHLSSVHALESGAAFSNGVTTGGITKQNFSINGDINESSNCDEDRVCENINWKFVNNPVDKLEVIVDRMRFNNMISDFQGLKKYYGGFPSAPELPAIKELELVQDFTKIHNQLIKKQQRLNANLVPDNDSGNGTDPHTDTGGVPGGAAGGTAKLNKQKRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGJ1</accession>
    <name>A0A1E5RGJ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Oxysterol-binding 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1378</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out)</text>
            <dbReference type="Rhea" id="RHEA:38663"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:38664"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the OSBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ85994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGJ1">
        <property type="interactions" value="1316"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGJ1"/>
    <dbReference type="InParanoid" id="A0A1E5RGJ1"/>
    <dbReference type="OrthoDB" id="14833at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005548">
        <property type="term" value="F:phospholipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120015">
        <property type="term" value="F:sterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030011">
        <property type="term" value="P:maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000007">
        <property type="entry name" value="Oxysterol binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2720:FF:000001">
        <property type="entry name" value="Oxysterol-binding OBPalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF102">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="51"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00050362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ85994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="466" mass="53237" checksum="DC718C7D7F56D43B" modified="2017-01-18" version="1">MSLNHKLKPGSVDGRNNMSRSSSVSKNSSINHSSTNSVAKNXSSSSIQDKDNEEYNNDDIDETDESGQNILLGIVSQLKPGSDLSRITLPTFILEKKSMLERVTNQLQQPDLILQAHASSDPLERFLGVVKWYLSGWHIAPKAVKKPLNPVLGEYFTCYWDLPNKQQAFYVSEQTSHHPPKSSYFYMIPESNIRVDGVVFPKSKFLGNSSAAMMEGLGVVQFLDIIDPKTKKPEKYTFTQPNVYARGILFGKMRIELGDHMIIKGPQYQVDIEFKTKGFISGTYDAIEGTVKDYSNKSYYEISGKWNDIMYIKGSKNGKKTVFLDVNKTHPLKPKVRPLSEQGEYESRKLWNKVLIALGKRDHKVATDEKFEIEEHQRLLAKKREEDDVEFEPKLFRKSTNPNDDLEYYVYKQIPATADKVEKVKSILEIAPVLPGQKFTERFQIPAYKKHEIQKQLLTENKKSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGK0</accession>
    <name>A0A1E5RGK0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="18">ATP-dependent helicase NAM7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g1402</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">RNA-dependent helicase required for nonsense-mediated decay (NMD) of aberrant mRNAs containing premature stop codons and modulates the expression level of normal mRNAs. Also capable of unwinding double-stranded DNA and translocating on single-stranded DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGK0">
        <property type="interactions" value="1256"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGK0"/>
    <dbReference type="InParanoid" id="A0A1E5RGK0"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21407">
        <property type="entry name" value="1B_UPF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18039">
        <property type="entry name" value="DEXXQc_UPF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21400">
        <property type="entry name" value="ZBD_UPF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000097">
        <property type="entry name" value="Regulator of nonsense transcripts 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040812">
        <property type="entry name" value="UPF1_1B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018999">
        <property type="entry name" value="UPF1_CH/ZBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF364">
        <property type="entry name" value="REGULATOR OF NONSENSE TRANSCRIPTS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18141">
        <property type="entry name" value="UPF1_1B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09416">
        <property type="entry name" value="UPF1_Zn_bind"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51997">
        <property type="entry name" value="UPF1_CH_RICH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 18" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Upf1" evidence="17">
        <location>
            <begin position="72"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="30"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="C4" evidence="15">
        <location>
            <begin position="186"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="42"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ86038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1004" mass="112625" checksum="00192A471A60A13D" modified="2017-01-18" version="1">MVIISDEFIPTTNDELTETSTHLNTNKTESASVNDANSNVGDDLEDSVELNGESENEXKEETRFQTSAHQHEIPQKEEACSYCGIDSPKXLLKCNICNKWFCNRKPEISFSSTTGNNPDGSNENTAQAIDAVQTNEIGENTAIGPKSIANFNNRSSSHIVNHLVLSHHNTVSCHEDSDFGSSVLECYNCGCTNAFLLGFVPAKSDVIVVILCRIPCGQQKNSNWDXDKWQPLIENKQFIPWLCELPSAEQIVKSNMITLSQISKLEAKWRLNQQATIMDILDFEEVEDEVIPILMRYHDAFQYQRSFAPLVKLEADSDKSLKESQALEHISVTWXESSLSSSSHEADLPSSYLASFSLSTYESSGLKVAVGDEMILRYNGPEHSPWEASGYXIKLPNAHRDQFTLELKKKPPMELMNGFTAEFVWKGIPYDRMQEALKKFAVNKKSTSGYLYYSILGHEIPSTGFDSRLPASFSIPGFPCLNQSQETAIRSALQKPLSLIQGPPGTGKTFTSATLVYHLVNIHKEKVMVCAPSNVAVDHLAMQLLELGLKVVRLTAKSREDIESSVSQVALHNLVLKAATGEFKGLLNTKQTTGELNNEQYKKLVNYFRKYEASILKNADVVCCTCVGAGDKRLGSNFRTVLIDESAQAAEPECLIPIVKGARQVVLVGDHQQLGPIILNRKAADAGLKQSLFERLILLGHIPHRLEVQYRMHPNIAEFSSIMFYEGSLQNGVTMEQRTLPRLQFPWPSPSIPMMFWANYGREELSTNGTSYLNRIEAMNCERIITKLLTQGIKPEQIGVITPYEGQRAYIVQYMQLTGSMNKDLYMDVEVSSVDAFQGREKDFIIFSCVRSNDQDVIGFLKDPRRLNVALTRSRYGLAILGNPRSLSRNVLWNHLLTYFRSKGCLVEGSLENLQMCTIQLNKASTMKKYEPQTSYINKYTYEGDDAITSNAQNERSNSIDTFDTHSLLSFNDMDMNDPASLFQQNNDLSSFLSKNFQNSLNFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGU1</accession>
    <name>A0A1E5RGU1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein transport protein DSL1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1565</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGU1"/>
    <dbReference type="FunCoup" id="A0A1E5RGU1">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RGU1"/>
    <dbReference type="OrthoDB" id="534815at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2230">
        <property type="entry name" value="Retrograde transport protein Dsl1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021876">
        <property type="entry name" value="Dsl1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021875">
        <property type="entry name" value="Dsl1_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038442">
        <property type="entry name" value="Dsl1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046362">
        <property type="entry name" value="Zw10/DSL1_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11989">
        <property type="entry name" value="Dsl1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11988">
        <property type="entry name" value="Dsl1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrograde transport protein Dsl1 N-terminal" evidence="2">
        <location>
            <begin position="23"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Retrograde transport protein Dsl1 C-terminal" evidence="3">
        <location>
            <begin position="681"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="435"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="471"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="471"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="507"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ86076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="889" mass="103261" checksum="0EAFB283F784E99B" modified="2017-01-18" version="1">MLAVIQNIQNTISDIQENIDAILKDQEHIQLEIENDPKFNELFLAKGQENDNSNAIIAHETKNPTIDKKSDILSKDQEYITQIEVLRSLQLIARLIEEFEKCLAKWSFEDAYVKLQQMCEKHKTLWQSFPLQLSLQLQKSLTSKLDELHLDFLEKLVKVYKFFFQTNEDKNELVLNVVSEDGNVSLKDVHTFVVSSLLEHKNIENVTKENSTLFIDTWFIDSIVDSNSVMEHCLSLMNGVAIKYIKLNNIIDRYIKYVLFHNSVCLSITKNSGLCWSVETSTLSSNEKVPFERQVENMQNVAALYDFLMVQNQEYYNFSKLSQTLVKQFENFIKLNVKPLWNKETNFKKEIIKIVEKSETLSRSNLLNILLDRSGNTYNSIIMDKLIREGFVELRNLFSDTTVLEKTRFVSLPRKVNDEAYLENSNEEISVKKIESQKSDISESELDDGWGWEDDVLEDNNDQSFEADTTIREDSVELPHKSNQMTLPEKSATTTKKTKNTDWADSLNEEELEADDGWNVDDDMLSLNDNKSSHEPAHKNTGTGKDSEYGDETDAWSINESLNLDDDAEMQKSESKKSDDKDVLETKGKVAHDEFHVSEATFKFLECWKLLNTRIANELPSCNSSEINKEYRLNLLLTSYYAMCTSQYDGDWTKYVNDIEYLKFEFSHNKDFQESAVSNISQLFHLSNNLVDNKMQMIEKRLYSLTFLQLKIIKNHESMGDPDWSVTFDELLPFLSSIFHNQILHVGVLEKKIQMVLEMTAFFYEKCIIKPVSEWNVISEIASENISELIQLISQNLDDILLNFFTQESVSKPHRQQFMNLRERVTLVAKVLTARLKDIMEMFYNGDFYLFETNEIVNWLVLLFADTRKRSDCIEEIKAIRAEAESEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH59</accession>
    <name>A0A1E5RH59_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein MPE1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1335</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RH59"/>
    <dbReference type="FunCoup" id="A0A1E5RH59">
        <property type="interactions" value="162"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH59"/>
    <dbReference type="InParanoid" id="A0A1E5RH59"/>
    <dbReference type="OrthoDB" id="106784at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16620">
        <property type="entry name" value="vRING-HC-C4C4_RBBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014891">
        <property type="entry name" value="DWNN_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033489">
        <property type="entry name" value="RBBP6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025829">
        <property type="entry name" value="Zn_knuckle_CX2CX3GHX4C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15439:SF0">
        <property type="entry name" value="CELL DIVISION CYCLE AND APOPTOSIS REGULATOR PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15439">
        <property type="entry name" value="RETINOBLASTOMA-BINDING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08783">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13696">
        <property type="entry name" value="zf-CCHC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01180">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51282">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DWNN" evidence="9">
        <location>
            <begin position="5"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="8">
        <location>
            <begin position="201"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="406"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="413"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="471" mass="51951" checksum="E07713A5C19462A5" modified="2017-01-18" version="1">MSSTIFYRFFSQKATSRVPFDGTGITVFDLKREIINMNNLGDGMDFQLKMYQPDTMEEYEDDTTVIPRSSNVIVKRTPAVKLLGGGGNAGVGGAPASVMRNVNATRYVTGNPRFLKPMAHSQNKMPATQLPQQKLAAANSQNMTEEERIASMFATQENVWEQTQKEMSEATPIYNKPSGVGAGAGASDPSLSQPPPPGYICYRCGSKDHWIKNCPNNNPILEGKRIRRTTGIPKKFLKTVTVDPTSMSAEELATTNLMVTQDGKFVVQMEDQRSWEDYQRKQQQQKLTKGENAKWSEGYFEDLPDHLKCPLTHGLIKEAVKTSCCAKVVSKNKMEDLLLDADFVCPLCGDTDVLLDSLEPDEKIRAEVKQFLNDHRQFINEKEKLGESQFDDEDEEVVGADGLLKDDAGSSAGGQANMGTNVSGGTHEPLAKKQKIMSPPVGMPFLPFPMFPPGFMPMMPPPPPPPGSSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHC1</accession>
    <name>A0A1E5RHC1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1264</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHC1"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ86309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="215" mass="24571" checksum="4A125CADC2721BCA" modified="2017-01-18" version="1">MTVHSLSKKPIELEHFXKKQRRDTSKRLDRVXNVLELKKYGIDVGFGKNKSKGRQKSHYAEELPKAILPGHXFMINVPLHGEIMKIKQTKFSELAKEGRLKLTEALGNGLIALYKLELENKSKSDVLLEAQFKASIKIRTAISKTIQSYFPNGPTVAKVSHKNCQDKAFIRKYNREGLFSIIGYLSNSEDPKKLNDLLNDKFVKPILEKVIKNNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHC4</accession>
    <name>A0A1E5RHC4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL3m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1109</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHC4"/>
    <dbReference type="FunCoup" id="A0A1E5RHC4">
        <property type="interactions" value="881"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHC4"/>
    <dbReference type="InParanoid" id="A0A1E5RHC4"/>
    <dbReference type="OrthoDB" id="274683at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000004">
        <property type="entry name" value="50S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_uL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019927">
        <property type="entry name" value="Ribosomal_uL3_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_uL3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03625">
        <property type="entry name" value="L3_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11229">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11229:SF8">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL3M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="284" mass="31524" checksum="CF276FBE9A6269E3" modified="2017-01-18" version="1">MFKSNLIKQLVLQRAQSTAFKKPQLVAPEIALAQVKPQLNILKHSPEAALIRKRLPYRSGVLAKKQGMVPYFDEKTGNRYPATVLEMNNVEVILNRTFAEHGYFANQIGYGDVSPKKVTRALLGHFSSKEVNPKFEVAEFRVKNEKGLLPPATLLKPSWFREGQYVDCKSVSKGKGFQGVMKRHNFHGQPASHGNSKTHRHGGSIGQNQTPGRVLPGTKMPGHMGHQNRTLQKLEVLKVDDKLGVILIKGSVAGPKGAMVKILDTIKMDPLFGIKKAEYKASKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHC6</accession>
    <name>A0A1E5RHC6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHC6"/>
    <dbReference type="InParanoid" id="A0A1E5RHC6"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="180" mass="20097" checksum="70C7BA8CACAB7256" modified="2017-01-18" version="1">MKRSHDEEELDGSKVVNSNGNHASGDNTSNGNTANHINNKKINTYSNKQSGSRHDGIMNMLMNNSRMRTNEMGDINNRTLDNRSSAGCTDNSTKDTRSTTRSTRSSTQNNGHQSKHVGKCYYCYRDVVVVEEEKEKEYAVCCVVCGNVTCGTCGIICYDHGCERKCLNCLYESRTKQESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHF6</accession>
    <name>A0A1E5RHF6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Allantoate permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1235</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHF6">
        <property type="interactions" value="149"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHF6"/>
    <dbReference type="InParanoid" id="A0A1E5RHF6"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF1">
        <property type="entry name" value="ALLANTOATE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="422"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="452"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="483"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="544" mass="61100" checksum="9E7A61862DB0B13C" modified="2017-01-18" version="1">MTDNSSCSEASXHDDKIRSVLSIADSEVGDPNAQITAIISPTSNKEVFLTGDIDDAMKLALEARGLEVSPKQDWKLVKKIDLYMFPLLSLQYAMQFLDKTSTGLAAIMGLREDLKMHGEQYANSASLFYYAYILGLFVLPPLLQKTKHLSPALCVIIIFWGLIQTVQSAPNINYAGFCVLRFLLGFFESAITPAFTVITCQYYKNSEQFLRMCMWFGSNGLGAILGNAISVGLIKHIEDGGSYAINPWKLLFLITGLMSIFLAFIMIFHIPSDPSRAWFLSKREKLMVVKRIQVNQQGFGNHHWKWNQFKEALRDWRTWAYFAYAVSFNIPNGALTNFQSILIKGEFGFTTLKTLEMNMIGGAVEFVGVPLFALIHTMLYNRGYKFFGNRLVMACFVNILVAVAACMLAFAEDKYAKLAGVYMTRVSSVAFVCVLSNIGSNTLGYTKKWTVTSLYMVAYGASNIAGTHTFITKQAPQYIGGKISIVVCYFTGCVILMLIWLGNVRENKRRDKWAEEHPDEGANVENLAFHDLTDFENPQFRYAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHG7</accession>
    <name>A0A1E5RHG7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphopantetheine adenylyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHG7">
        <property type="interactions" value="161"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHG7"/>
    <dbReference type="InParanoid" id="A0A1E5RHG7"/>
    <dbReference type="OrthoDB" id="330671at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004140">
        <property type="term" value="F:dephospho-CoA kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02164">
        <property type="entry name" value="PPAT_CoAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000089">
        <property type="entry name" value="Bifunctional coenzyme A synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001985">
        <property type="entry name" value="PRK00777.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695:SF46">
        <property type="entry name" value="BIFUNCTIONAL COENZYME A SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695">
        <property type="entry name" value="DEPHOSPHO-COA KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="1">
        <location>
            <begin position="138"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="288" mass="32313" checksum="13906C4540D1B9BD" modified="2017-01-18" version="1">MLALLIIDTIHFEDLSKNLQPVYDQCGNPDIQKITVLVTPAINSANLLDDVLGYLXTKCRTAVNGYSKNIDVLFNVDVGRYIATTECNLILAPKRLHLQHENLTQYEIEYAKELEGCSVDSEPKQDFYVEGYYKVTALGGTFDHIHDGHKILLSVAAFLTSQKLIIGVTDQELLENKKFKQYLQTFEKRVEYVLQFLKLVKPDVEYQIVPIRDVCGPTGTVPEIEALIVSEETVQGAGIVNKTRKERGLEKLEVFVVNILGGGKDWEEKLSSTYIRELESTAASKSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHK9</accession>
    <name>A0A1E5RHK9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ER-derived vesicles protein ERV29</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1015</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SURF4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHK9"/>
    <dbReference type="FunCoup" id="A0A1E5RHK9">
        <property type="interactions" value="542"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHK9"/>
    <dbReference type="InParanoid" id="A0A1E5RHK9"/>
    <dbReference type="OrthoDB" id="7859621at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002995">
        <property type="entry name" value="Surf4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02077">
        <property type="entry name" value="SURF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01339">
        <property type="entry name" value="SURF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="217"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="240"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="8"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="325" mass="37194" checksum="12EF7DE98AAEE1A0" modified="2017-01-18" version="1">MSTFRGANYNNNNNNNNNGNLNGGSSNFQRLGNQGNTKKSPLDKIQAVLNDENFRKNYLQKFENFTDRIESYSDLPIFELLKPYTPLIARLLIVATFYEDSIRIVSQWKDQVFYLWNYRHIPYFLVLLFLSLISVTMIISSSMLILRKKIVYATSALIITVVLQAFVYGLVSGFTFVLRNISVIGGLLIALGDSIVTSKGTFGLLPELHNKTSDMKNFILLTGRILIVVMFVTFSITKNWITVILTIFGTLLISIGYKTKFASILLLLILFFYNITINNYWFYKKGKRDFLKYEFYQNLSIMGGLLLTVNTGAGKHSLDEKKKIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHT0</accession>
    <name>A0A1E5RHT0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1387</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHT0">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHT0"/>
    <dbReference type="OrthoDB" id="4082971at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="207" mass="23593" checksum="6347F8A058369CDF" modified="2017-01-18" version="1">MDTATAPLPSRPSASPEQVTVSLLQEQLEHIKLNENAKKKADYSEILVPTAGGDPNVPLKNKAVTKPIELDSSTGEVLVRKATGKTKLRKGQSEQEYQEQKKIYQDNQGPLRKEEGWXAVTNENIPLDTETQREHCVVDKQKRALLNSRVYYYYYRKDYQMCVKLVSQLVELYTSDPKFIKLCKKDIHDLQDVHSLCIAKLETDTNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHT6</accession>
    <name>A0A1E5RHT6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Vacuolar basic amino acid transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1443</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHT6"/>
    <dbReference type="FunCoup" id="A0A1E5RHT6">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHT6"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF47">
        <property type="entry name" value="VACUOLAR BASIC AMINO ACID TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="265"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="301"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="426"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="464"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="537"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="47"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="575" mass="64088" checksum="7F9F26727120BD91" modified="2017-01-18" version="1">MRTDENTPLIVRPELSDITEEVEETANFEELENEYQAFNLNLPKAPILIALWLGSFLASLDNTVVANMMNVVSEEFHQSEQKQWIATSFLLTNTAFQPLYGKLSDLTGRKFALLCAHFFFGIGCFLTIFANSVEQFSIARAICGIGAGGLNAVSSIVVSDICTAKDRGFYQGYANLVFAGGQYLGAPLGGFLIDAFGWRFVFAVQIPMILICFVLTWTNVNIKLAHVPKKGRFSFENLSKIDLGGSLSLIVVIGGILILTSDTSIGISNSCVVLITLASCVVFLMNELYWCKERILPFQDLWGKFGVISLLTLTSSFVAFGEIFRKPVYLQLIQNFTNSDVGVYTIFTSFASPLASFVTGWILKKTSMNLEYCSYLLIASSFIFQVFGLGLESWFLYNLPPNVGNHATTATAVQFFLDNSKVYWKFILVFTTCLSYYGYCTLLVATLVSIVFSVEKSQQATFIGLFYLWRSIGNVLGASLTLTVFEKCLDVSLFKYFKNIDDLSVYKKLIHDSSLIRKILDGQMLIDVLHIYNNSIIVAYFPSIFVVAISIVIALVFIKVTQKQMADEDFKEVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHU3</accession>
    <name>A0A1E5RHU3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1416</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHU3"/>
    <dbReference type="OrthoDB" id="4032425at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="456" mass="53061" checksum="D30D70B5776A0209" modified="2017-01-18" version="1">MDGKTKPDMDNELSFDPVLFLTLPPLIRFKIYSHFACKSDKTLCDFTSPQLTPPNPSALFLSSSTEFAAQSVPHCLQQVQTQKLLQNYVERFPVVSERDSLCTLAEMEDHFPEIRGFLTNFLYMSFWLPYDCIFLDYLRLNREEDFWYLKVEEPADKVEGPTFTTDWTKWVLYKKNASRLIFAQYTMFEYLKWIQNGSYGQDNLICVCDDFFTLTGLEAGGDESGTRKRETNDRKDQSVGAQEDGSFLPWLKIINMCELSAQVPVERLXRFKYFENLRALKLQVNDFRNFQCVMQKIIECKFHLESLSLDVSSWMSESFAQQWYRSHYFNGLAVRHLEITGINRALGLYTSEEDSKFFELETFVKIQLRFNAALQSLVFRDCCDLQWFDTCTTGNETTVLQLLAVQRLSIKFYNCNIVPKNQDQLLCTPECIESNLATGSSKSAMNLHKSSIFFYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHV4</accession>
    <name>A0A1E5RHV4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Succinate dehydrogenase [ubiquinone] cytochrome b subunit, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1481</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHV4"/>
    <dbReference type="FunCoup" id="A0A1E5RHV4">
        <property type="interactions" value="367"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHV4"/>
    <dbReference type="InParanoid" id="A0A1E5RHV4"/>
    <dbReference type="OrthoDB" id="588261at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03499">
        <property type="entry name" value="SQR_TypeC_SdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase, transmembrane subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018495">
        <property type="entry name" value="Succ_DH_cyt_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014314">
        <property type="entry name" value="Succ_DH_cytb556"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000701">
        <property type="entry name" value="SuccDH_FuR_B_TM-su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02970">
        <property type="entry name" value="succ_dehyd_cytB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10978">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE CYTOCHROME B560 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10978:SF5">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE CYTOCHROME B560 SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01127">
        <property type="entry name" value="Sdh_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="Fumarate reductase respiratory complex transmembrane subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01000">
        <property type="entry name" value="SDH_CYT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01001">
        <property type="entry name" value="SDH_CYT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0830">Ubiquinone</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="199" mass="21632" checksum="2D92FB2901609832" modified="2017-01-18" version="1">MFRVVGLQSSSMLLAKSSLASTLSKARAATSRTASSAAFTRAMRTAAPGKRFMSTEATSIKEANKILVNQRAHRPVSPHLTIYQPQITWYLSSVHRISGVFLGMAFFGATIAFGVSGLLGLGLTTDKLSEFYHTHVPKWMDYTVKGGFAYLFAFHFGNGIRHLIWDAGKGFTIKGIYAGGYAVMALTVVAGTNFFLKSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHW5</accession>
    <name>A0A1E5RHW5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">DNA replication regulator SLD2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1415</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Has a role in the initiation of DNA replication. Required at S-phase checkpoint.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SLD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHW5">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RHW5"/>
    <dbReference type="OrthoDB" id="8775810at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902977">
        <property type="term" value="P:mitotic DNA replication preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22289">
        <property type="entry name" value="RecQL4_SLD2_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1460:FF:000001">
        <property type="entry name" value="DNA replication regulator Sld2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021110">
        <property type="entry name" value="DNA_rep_checkpnt_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040203">
        <property type="entry name" value="Sld2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28124">
        <property type="entry name" value="DNA REPLICATION REGULATOR SLD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28124:SF1">
        <property type="entry name" value="DNA REPLICATION REGULATOR SLD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11719">
        <property type="entry name" value="Drc1-Sld2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="146"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="388"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="548"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="391"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="420"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="452"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="552"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="600" mass="69422" checksum="C451059C0171E804" modified="2017-01-18" version="1">MITKLATSLQSSQSPEQKNPTEPRPYKKQKPENTYIMAISSLSRRASTLERSSSTLNNGDHKTAIIMSQKSTATRTKPSRIQTHYEEKLLHLKVELKKWEYRFISKNKRSPKKTDIQILPEVRKMYKEYSTLKKLAQNEQSIQEKKRLLNEQPTPSQKWKPTQKXFQRVTPVKKQDNESADELLDWNLGPTPQIHGKVQSILDVFVSPLKSTPNAKLSLNSPDKKINMSQAKKKLDFGLDETEEEENVFGCEEPISNGNVNDDETLSVIEESYSGCSPLKPLQLEAPKQALRKQINSPNKYLQNVSCAVRRTPVSKFHCNESNKGDYASSVGSPSPLIKLVSQNNNXFHKSLFELAKEREQIMKEFQSSGISVDLLGQLKNEQVYDYNSTENDELEEEEEEEEVDKPDEKEDVEISYEQMETRDEKVDTADQDFEIKKIEKENSKDQTVVAENEEVGDEDINQEELEEATLPEAKSVQKKSKRNSKYENTAANNEKLLKQRRRKIIKVHKNKTSTKKQELAEQKIMKKDLHEELRKLKQKSVNKFYGVPSAQDDETEDEDFIDPIAKKNPKKNKYNLVSNNFVRMKIXKKRNPNFRNFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI01</accession>
    <name>A0A1E5RI01_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ubiquitin-like protein SMT3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1610</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI01"/>
    <dbReference type="FunCoup" id="A0A1E5RI01">
        <property type="interactions" value="1158"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI01"/>
    <dbReference type="InParanoid" id="A0A1E5RI01"/>
    <dbReference type="OrthoDB" id="442921at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000155">
        <property type="entry name" value="Ubiquitin-like protein SMT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022617">
        <property type="entry name" value="Rad60/SUMO-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10562">
        <property type="entry name" value="SMALL UBIQUITIN-RELATED MODIFIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11976">
        <property type="entry name" value="Rad60-SLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="34"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="111" mass="12412" checksum="A687CB1C26AFDC8A" modified="2017-01-18" version="1">MSEPNSPSNPTNNEVANTNEPNSDVKPDVKPSQTHINIKVSDGSSEIFFKIKRSTNLRKLMQAFANRQGKDVNSLKFVFEGVRITGDHTPDDLAMEENDMIEVYREQLGGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIB9</accession>
    <name>A0A1E5RIB9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="14">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g1160</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="14"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RIB9">
        <property type="interactions" value="525"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RIB9"/>
    <dbReference type="InParanoid" id="A0A1E5RIB9"/>
    <dbReference type="OrthoDB" id="292747at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23285">
        <property type="entry name" value="beta-trefoil_MIR_PMT4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000044">
        <property type="entry name" value="Dolichyl-phosphate-mannose-protein mannosyltransferase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF51">
        <property type="entry name" value="PROTEIN O-MANNOSYL-TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 14 16" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="222"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="593"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="638"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="668"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="718"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="15">
        <location>
            <begin position="333"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="15">
        <location>
            <begin position="401"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="15">
        <location>
            <begin position="466"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ86637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="764" mass="88333" checksum="5DBC40DB2F68D721" modified="2017-01-18" version="1">MSVTNRGKKTVAAGSPPPNAVVTEADESKYTKGKYDYLGSKWLLRAKPDSESTYLFWLYTVTFVAAAVRFYILNFPKEVVFDEVHFGKFASYYLQRTYFFDVHPPFAKMLIAFVGYLCGYDGAFKFDDIGLSYEKNRAPYLAYRGLSAIFGTLTVPIMFLTLKEMNFKAVTCFIGSLLVALDNAHVNDSRLILLDATLIISVALSIYCFVKFYKEQLRKPFTSAWFLWINLTGISLSLVISTKYVGVFTFMMIGTAVAFNLWQLLDIRSKTSVNDLLKHLLYRIQALILCPFIYYLFWFFIHFQILTKSGPGDDFMSPEFQQTLGESPLLRDARDLNYHDIITIKHLDTEALLHSHVERYPMRYEDGRISSQGQQVTAYSFEDFNNQWEIIPTKQLSTTLGQRVHLSDVVRFKHLGTNTYLLAHDVASPLYPTNEEITTVPEEEANGERFQQTLFRLEASGKKPSTDAVKTKFTRFKIVHVDTSVALWTHNDQLLPDWGFEQQEVNGNKKIADSDNIWFFDQIIGLPKNRDVHVIKQISKMNFFKKWWEAQTKMFAHNNALSSEHPFASNPESWPGSLSGVAFWNKDSEKKQIYFIGNIVGWWMQVASLVLYVAIVISDLVTRRRQFYVFGKLARDKLYGPITFLFLGWATHYFPFFLMARQKFLHHYLPAHLIASLFSAALWEWALTEHNSLDLDKDEEEPENPYETNPRIKLAKMYFFYAVITAALVGFFXYFAPLTYGNVSLTPEQVQNRKWLDIELHYAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RJ66</accession>
    <name>A0A1E5RJ66_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Vacuolar protein sorting-associated protein 70</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1183</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RJ66"/>
    <dbReference type="InParanoid" id="A0A1E5RJ66"/>
    <dbReference type="OrthoDB" id="5841748at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039373">
        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036757">
        <property type="entry name" value="TFR-like_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF46">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47672">
        <property type="entry name" value="Transferrin receptor-like dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="800" mass="92590" checksum="6BEDCE10FFC421AC" modified="2017-01-18" version="1">MSSTPLLQKQPSSYGTLHRIINNNNKXISGTNLSASNNISSEDLTQRDEDIEWPYEDNERELSDANKNDHSPSPFSRAARFTRENSIISTLARKRGDTLTSIKEGYFKVVRYKHFKLFLSMFIGCLFFYISFTLAFLPRTSLSRDFRRIHFSQQLTKPEIYRHYMDSMRQDNLFPQLVRNFSASNNLIGDEILLQSTYTYLDSKLDVKPKLEQYDFKKDLYNTDNEISLFDSQSGKTIYKPNIKEPCFDSTSCDDNSLKSFVFSQLGPFSIDGHGVEVDMGTFDNYTSIKSDSTSTLSDLQGKIHILKLNNEISLMSQIRNSISFGAIGCIVYSEKNVDDQYYFQNNYISRNSIINDETXFKIPVISMSYNEIEPILKYIRNAELPLCYKIHLESKRYPALQEVAQLNNIVVTIPGIWKSHEVIIGHNRDLFTYHGVNSGHXIFLELCRGLSALKQKGWKPLRTIKLISFDGDTYGNFGSKNYLKQHLNNEKSIVFIDIDKEAVTGNSTFSCRSSEIFNGLIKTAAKLTPYSKNTNNDYDSDLFREFLEVDDSKRLNLQELHADSRAQLFLSDYGVPSISCGFSGPITFPHNSNYLSMELFEDLVDPNYNSHQVLSEFLGLLVLTIAEREVIEYDMGKYFTEIKFVFDELIESHSFMDRSKEKYINKLDKLLEDLAIIFNSIDLYNARLQQQTSIDYPWYHLNTKIQLMFKIKSLNNKLIKIKQLFLKGTYEDTRHPKDIIMQPNKYTGNSIEVLGTLHELLESREKHNGSQQNIHEWLQTLYEKLSVIKEFTLDIYPYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RJ96</accession>
    <name>A0A1E5RJ96_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1167</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RJ96">
        <property type="interactions" value="694"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RJ96"/>
    <dbReference type="InParanoid" id="A0A1E5RJ96"/>
    <dbReference type="OrthoDB" id="3971810at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050869">
        <property type="entry name" value="H3K4_H4K5_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197:SF251">
        <property type="entry name" value="EG:BACR7C10.4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="2">
        <location>
            <begin position="482"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="694"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="749" mass="85126" checksum="F363DFC57F3B5BB9" modified="2017-01-18" version="1">MQNXALCDIENHTCQDPHFEQQQQQQQQQQQQQQQQQHPLITVTSPDDSIGNQLETSTKHENHDXEISPSMGASYTNHDTQTNSYGRESFHLFNTKSGEVPRVEILSLNDEQEQGQDGANKQHTGHMFHRNGSPVYDDIAIVSDSESDYDSDSNQLRSDQCHDTNGGNYTHGPSWEQSTNQHAXHLQPTAEEDLVMEKFHIVPPENFQPVNDVPNGFHVEYLQESSVRSGESLDITTQIPLIKADIPINVQENISSDLPIEEKLSKLEKVEPLFLYEVPNFLQIPSLESLSLIDIGVCCAGCGINLANFANAITHQLIAGTNLAINSSAATTKVARSKSVSGSVKQGLDCEDCPAMYCSMVCKKTYKHLHKNLKHPSLKFRARVLSSVENTAQNRLKAKKHDQYDKKKDIEKQVEKENEEDEGPPPPTNYRIWNSKMEEFIKSHFWNSGYNLAMYLINIENMDTSKYGDVHKLGSXQLIKDQFLTVSLTXEDQKHNVDKSYVSIDDALLXGEEEKALKEEESTERNQLKMLREGYEIFCEMFPKYQTKKYKLAFSEFKQLIDSIEYNSQICDRVPEAYGLNIPILSMFQHSCYPNCVVLKQMDNLPNSAGASKSSPKQAGKTVRYAIKPVRSIRSNEILTFSYVSEKHSEEERNFELKYKFGIEKCHCDKCFEDRNKRFQSSYELSLSSLDLSRAGSTQSSRENTVEEDSHKHEAHRRKSSMKTGSKGKCVEGRRPSVRFDGKVVALNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN27</accession>
    <name>A0A1E5RN27_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g715</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN27"/>
    <dbReference type="FunCoup" id="A0A1E5RN27">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RN27"/>
    <dbReference type="OrthoDB" id="4064753at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ88282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="312" mass="37381" checksum="F5370CCE6D933BFC" modified="2017-01-18" version="1">MPDEIQKKPRTTVQTENSDFFSNKTLHQTLKYIWINYQEMRNSKTLWAAIKQTSTRDSSIQPPVFINNTLVNKSRLYTCVNQIASGSATINPDFCTESRLRQLTKQESTVFQWYFHSKGIPKLLEDRTYKIYMKKKWFSLSDNMKTLYYYDYFTKCLRIDYHILSTYKFARLLEWEIPPTSPYIMFRQHFKTSYAQRMDSRSELGIMKSLSTPKKFCGVDKNKITTKQYLNNRTQTRKFPLVETVMKYDNTKRNKYSRSDLYRACKKSWEEKISGDHKKALEKRLLEKYNLLSLKVQQEISKMDRLIWRSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN48</accession>
    <name>A0A1E5RN48_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sorting assembly machinery subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g872</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAM50/omp85 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN48"/>
    <dbReference type="FunCoup" id="A0A1E5RN48">
        <property type="interactions" value="159"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN48"/>
    <dbReference type="InParanoid" id="A0A1E5RN48"/>
    <dbReference type="OrthoDB" id="1724197at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.50">
        <property type="entry name" value="membrane protein fhac: a member of the omp85/tpsb transporter family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039910">
        <property type="entry name" value="D15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815:SF18">
        <property type="entry name" value="SORTING AND ASSEMBLY MACHINERY COMPONENT 50 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815">
        <property type="entry name" value="SORTING AND ASSEMBLY MACHINERY SAMM50 PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="domain" description="Bacterial surface antigen (D15)" evidence="6">
        <location>
            <begin position="139"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="447" mass="50275" checksum="8AAB63267F8A0CAE" modified="2017-01-18" version="1">MEDQEKIQALINENPHYPVVLTSINTSNLPFNATILRDYVQHTIQNATTMAEYIQHQQIFNEKLISNGLVENVDQNLTVQLLNKQTPKPLSSSMVAQSVPPLGLSPYYQFTPVKKFSAKTGSKIGNGEGDGYLEFQLRNYFGTGEKFIMDVQKGTKTKSSVLLQYQQPFKVWYNTSLSFFQNNRKVSGCTYELSGLKFDFLTSYHRFSGKHVWNHAISFESCARTMKNNSRMKSLSVLFQCQEDWKNSLTYTISNDTRDNHVSPTMGHLLKFSNELEVTTLWNKFQLEASKATSTILSSGRQVSFIGTLKGGTFASLATASPYVPYIDKFQNGGGNDIRGFDFMGLGDKDHVDSLGGNHYMSYGLSMMCPLKPGSNFKWHLFFNGGKLCNDAKKLVKVQEHSTAIGFGILYSHPSARFELNFTLPISASETDLQRKGFQYGLGMSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN75</accession>
    <name>A0A1E5RN75_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcriptional regulatory protein SDS3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g869</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN75"/>
    <dbReference type="FunCoup" id="A0A1E5RN75">
        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN75"/>
    <dbReference type="InParanoid" id="A0A1E5RN75"/>
    <dbReference type="OrthoDB" id="70376at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013907">
        <property type="entry name" value="Sds3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21964">
        <property type="entry name" value="BREAST CANCER METASTASIS-SUPPRESSOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08598">
        <property type="entry name" value="Sds3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01401">
        <property type="entry name" value="Sds3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="269"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="361"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="231"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="275"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="306"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="362"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="373"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="420" mass="47410" checksum="E9F48A7B924E02B2" modified="2017-01-18" version="1">MAQADNVTQQSHTIGEPVLMSQQQQHNTQMPPIKQTQHKTTKYKKKLSLEGKVNKIANKFIQDKDLHYKERLTRLQTNLTTMHQGNNVEFLRKVRDLEEERDMNLVELRYFEEYRVSRSSVEFDQDIEQLKTEHENLVTLCKKKLFEQLEYKIKMLQEEKLLIEVANSHAYSMDYSNIYNQQPQQHGMVTRNNRHLTRGESLRLDGDQGSDLNANHSSSFAGLFHSTGGYSSTERTGSPWLNESGSSADDLAKDSSANVFIGSRRSLRRRVTTKQAERMSKEQELESIQENGSGLYGKASQHKAGNKTAGNGNGKNSGNSNSNNTNGDPELSSEWVKLVSGYSQLLALLFGNTDEDGKNDYYNGSYNHSTGNTKKRNVGRHSTKSAPPLPSLEKDEVTDDINLIRSLTGQDPSPFTSSNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN82</accession>
    <name>A0A1E5RN82_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">25S rRNA (Cytosine(2278)-C(5))-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g852</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN82"/>
    <dbReference type="FunCoup" id="A0A1E5RN82">
        <property type="interactions" value="552"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RN82"/>
    <dbReference type="InParanoid" id="A0A1E5RN82"/>
    <dbReference type="OrthoDB" id="435282at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049561">
        <property type="entry name" value="NSUN5_7_fdxn-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048889">
        <property type="entry name" value="NSUN5_RCM1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF4">
        <property type="entry name" value="28S RRNA (CYTOSINE-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21148">
        <property type="entry name" value="NSUN5_fdxn-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21153">
        <property type="entry name" value="NSUN5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="3">
        <location>
            <begin position="167"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="452"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="276"/>
            <end position="282"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ88349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="535" mass="61993" checksum="402DD647E7AFCA4D" modified="2017-01-18" version="1">MEFYRDATWILEDLVKLSTKNNKNNNTDNDNNESSTNAKHKNQISGSLQTIILKSHKKYKLKTNPKHIYAVVQSCYHFLPLLDYIYKKSQIEQQIPVKNGKKLYTKYTLYLCIHDLLLSKSKRIQMGKHPLKKFVMNQKTRLNAELQRFKIKNKISNLNTYFLQQSAINQQDLPPIRWFRLNLVKLANKTEIEKTMHEIGKKFPQKVDSWKDIESQDVEKIYYDEYIPHLFGVSPLSKITSHELYKQGKIIIQDRASCFPAHILQPSHKDVIIDSCSAPGNKTSHLASYIYSDGYRPGSRVDGDGTHHQQQQQQQEQQQIIAFEKNPERAKILEKMLSVAGCTQKLVKVNVGDFTKIGIQKDYTHVTGLLVDPSCSGSGIFGRKFVDDLNKKNGKHKEENNQDEDDEEVPLEEFKQQEMDKQRLTKLSSFQFQIVKHALLFPNAKKVVYSTCSVHAEENEQVVLDLLVDNKVKEKGWKVRKREHVIPKWDRRGFVEEFDQTFDKETAVEIAQGCIRALPKEDGGIGFFAVCFERE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RND2</accession>
    <name>A0A1E5RND2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">CRE-binding bZIP protein SKO1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g663</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RND2"/>
    <dbReference type="FunCoup" id="A0A1E5RND2">
        <property type="interactions" value="763"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RND2"/>
    <dbReference type="InParanoid" id="A0A1E5RND2"/>
    <dbReference type="OrthoDB" id="295274at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14687">
        <property type="entry name" value="bZIP_ATF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051027">
        <property type="entry name" value="bZIP_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020956">
        <property type="entry name" value="TF_Aft1_OSM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19304">
        <property type="entry name" value="CYCLIC-AMP RESPONSE ELEMENT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11785">
        <property type="entry name" value="Aft1_OSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="6">
        <location>
            <begin position="565"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="110"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="172"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="315"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="433"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="13"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="29"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="54"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="68"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="122"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="183"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="205"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="315"/>
            <end position="325"/>
        </location>
    </feature>
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        <location>
            <begin position="341"/>
            <end position="352"/>
        </location>
    </feature>
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        <location>
            <begin position="440"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="494"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="505"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="542"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="695" mass="74287" checksum="A09EEC9F103E0649" modified="2017-01-18" version="1">MAAFDLEFEPNPFEQSFASKSPASVQTKSSSSNGDKSSVSSTAVGRTSVPVVNRSASTSVASVHNARNNITTTTTTTTTNTNIVNNKMSNDSVLLNSGTPIHEHHRANSAASGNAGAHSNHRNVQSFGGNDGSAGNNAKDRNGIGKIALPLNGAQMKPPVIVSPAILTPGGSRHLPIPTLQGSHNNNNRNHNHSNSQSHSHNHTPNRASSNSFHSSTMQSHSNNAFPNPDLNGSANSNSAIHNFSLPASNGALTPSGFFLNFSSLNSGSTSAKNGLANANFDTSIRTGLTPAILYPQHNQLHNQPLQSHTQGYNSQLLSSSQNGHQGKPPANIESTIDGINGSSSNFANQNNASIKPNFNGLLSGTASPGSAFFNQTPGGRLQPMLGLPLATFSPNNLSLLGTGGNSTPGILNVMPPIQISSDKTTAEMHELDAQVNQTKPKRAYKKSKTNKKEGKEQAVAKGNTATLKDKNGLNLAEKPAKKKRKKSAEQSENPTALQSQGTEAGDKEIGETKKTKKKSPTLKKEQRDGAVSTELSLENIEDWRTGSAGKDDFPGSEENMTAEERLQKRKEFLERNRLAASKFRKRKKEYVSRMERKLAFYKSEYKELTNNCLGPLSGISSVTSSPSSSLSHNGILDQLKASLSTGNYDAATSLVNQIQELLNNTAFIKRGGIDTMQIEDDQEERTKKEFDVNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNE1</accession>
    <name>A0A1E5RNE1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">F-box protein DAS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g991</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNE1"/>
    <dbReference type="FunCoup" id="A0A1E5RNE1">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNE1"/>
    <dbReference type="InParanoid" id="A0A1E5RNE1"/>
    <dbReference type="OrthoDB" id="2852960at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="17"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="780"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="780"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="802"/>
            <end position="832"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ88409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="844" mass="97725" checksum="4D881B9BF2ED7702" modified="2017-01-18" version="1">MFKKSTTKESPCGQGLGLPLNTFSDEILYEVITHMDQRTKLAMSLVNLRFHKLANKSIYRQIYLNDCCVVESKSINLANKWTVLYLPALIDEKKSRFIADKKLCRLLATFEEKPQTTLKHVEWVRINWDLNKNLQKLIVERLCNGAPNLVRLENVIDPSCYDIIAQGKYSDRNSSLRSFDMAPPNSSPGDTIDDHYLPNFYKFLDSRFYNNIDNITTLTLFIDPVILFGLNNYLDQETNFGLYPKLLKGGKKLYIKDLKLHWRLEFFPMNVFQLQQKATKMTLPKLSDMFDTKTLETLTIISWEEEMEERNKELLRQVADFHNLKDLSLISLQQSFAILQTLFDGKNMPDLKRLKFDFLDSFGLDNASPLLLRVILDNCKKLEYLDIRSNEFLVQPIVSLRRSMDNETNNREEYHHQNLRLFKFGVTQQCYCPKCCDYFNSFLTKTLFPKELDFNDINYMKLHEFFFRDIFKLMKHYGLFPYSKASDIYPSVRTQPMNISEFVKKFNLKLVLESGHPFMRRLRESMEPIYKKLFETILDMYVKVKAEGGFSSKYMMEQKPFAKVDQQIASETLPGDEYKSHFITGVDVVRSVHTLMHHNRKPYQYILSKLPNLKFLVFNDLPVAIVEESLNDVNFSGQKIRKRIQPAFFSSGYECNLYTDSVSDNKTLKGSTKTDLDLYKDIILQEELGGKDLDFKKEFGPYLIYPKILVEGVRKLGSISSLAASLGYPKAFTKLTSMDSIFDNMLTPFELENNETSENESSESEDSPFARLIQHDQAGTTPHVTNSAESLDDSNLFGEGVNGSSSDQYNQSEQNGDSSVQEEFRIVTNSSQEDVDMDSSEHSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNE4</accession>
    <name>A0A1E5RNE4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cyclin-dependent kinase inhibitor FAR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g992</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNE4"/>
    <dbReference type="FunCoup" id="A0A1E5RNE4">
        <property type="interactions" value="225"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RNE4"/>
    <dbReference type="OrthoDB" id="3973349at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1207" mass="138301" checksum="70A879C61119005E" modified="2017-01-18" version="1">MYLLKTPTNNKGENPLLTHTPPSATKSQRSKLFKTFSGSKVFKGEQKKESVSSLRLDTSSIKNSLSNKRQSTVTHDKLGLRIGTTPTDASFPAGFDSISEELNRHSKARNLDHGYSKKSHIPTPLNLEMLSPPPSSSKAKEMFPRFLEKLPHLRKSDAVVTKRPSKQNLGYLNLPIESFHDQKADLLKRNPRRNLLNSVEFREAADLETTDEKTPDFAENVYKNIETKGLITGMSSSSNLERKKNYTMNICFICDEALNSLLPGEKVLEMSCSHQCHYDCLLASLEGSSAFGERNLEYPACSICQQTSKPIEQEINDTLVRDLLLTSMNNDVLSDLNNFHVSRSSSSNLKQQKKTPITPTGQLIAAQKVTQHGYMKTPIKEHHDLTKRNLGQPNQQFHGSMIPKPVFSVNPQASTINVSLNSNKPLVIPHVLYVHLPDISKTKTVEQSKENALLRLDNLETQQKFSKLVLEEFFPTYSQNTTTASNNLKKENHEILQMIDLMEVSLDDVNYSEWLLVMFENKIILKSLKTNKSARNLQMGSLSSQVINISNKELYSIMKCKDKTFVSLKSSQIPELYMKNTENPIVLNKWTVVMEYKLGIKSANNDDKISHFDKSGLNLSFLHFTTNAFNLMQERGLLVYFPASLHAKITLYLNDPILCHNLIPKFESESIKDPLKNDSSEVSATVCKNGRVQVLVINVLNYDTLVNSTEMCQDIKTMVENFLNAAGKNDQIGIIVFGRDSENKMNLNEGTFLGMINNKWGGIAEFLKNLKVFDCFHVSRTYSEQERELHKITQITKRLFIMNNLFDARFSTNNVCYKKHIHIVSNSYNGTVSKDPQENQIEISRSFRKFVSDYKLNISLYSIGAIHASLLENIMEQHFEFDSRYRCFNYSRQGEIEFCKNSNSQIEDLMIDIKLKDNKNGFFKFHCLENSFGHKVQIENNENLPAVRVNLGAFARGTFKTYLLDLIIVNPKEFYKENCLKIENKGLIDVFADDSSIICRNADGNFKHTQSFIEFSTNWVDKDYGLINGDIYDSGKILINFYGISDFTKPMTDYHPFLNDTELLENDEDTEFLDIVLTAPLTSSRDSIYVLREIEMMIIEVMEAHLRCFARLNWQDMEHDFKQLYSVTFAMSRNIQIEFPSFNSYMAKFQVHNKYLTNYIEYLCRMIDDIFVFHGSSSRPLPGQEELSKFKLVRLLNNLKFQNRHNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNK4</accession>
    <name>A0A1E5RNK4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NAD-specific glutamate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g636</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNK4"/>
    <dbReference type="FunCoup" id="A0A1E5RNK4">
        <property type="interactions" value="103"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNK4"/>
    <dbReference type="InParanoid" id="A0A1E5RNK4"/>
    <dbReference type="OrthoDB" id="184415at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056365">
        <property type="entry name" value="NAD-GDH_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055480">
        <property type="entry name" value="NAD-GDH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF24">
        <property type="entry name" value="NAD-SPECIFIC GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23147">
        <property type="entry name" value="GDH2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23152">
        <property type="entry name" value="GDH_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="5">
        <location>
            <begin position="725"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="120"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ88253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1100" mass="125393" checksum="753159F7AB03FCEC" modified="2017-01-18" version="1">MTLTNNKETPLPDISSLSISSLQDYPNYEFPGKELQKEKIIDLIDAKGCVPEDLIENEVNWFYDSLNLNNSFFMKFDTLDEKSQDLNILSNIIISLYLAKIDSTLLSQSNDDINESNESTTTTTTTTTTTTTPSISPDPLKLKINKSFVNQEKGYAIFLDTDDTLDNQIDHEYLDSKKTSNNYKLEAYVSSQYNLKITIIESVPTTNDNSNNNKSTTFSPNNEKLYTLLKLECKEREGPTLKFLKSLNDDIVNEYRLIIAYEKNSTSNFYQSLFNLFKYYDIKPNKIYMDVIVNDLKSKLQQKLVGHQQFEDDENLNIFSIYFNQSDNPSYSTINDFNSTLTQILKESSLLYCLPSINTQVFNTKNQKTIEKTDSNTIGKLFENDPSPQEITYAYIGSIFINHFIDRFQNLGDLSNQHLSVDLLEILKKNWKSQSYSQTLINNVLAKHQPIVSRLFKNFALIHYPSTSFKNSTTTQHNTAFKKTLSYQRIVTGAQPFANDKDFENYLNKVIDDQTPDYLILKTLNLFNKSILKTNFFINKKIAISFRLDPKTIFQDVCNPLTDEIPFGLFMVVGSSFKGFHIRFRDIARGGIRIVTSKNSDVYELNSNSVLEENFNLASTQQKKNKDIPEGGSKGVILMNPTLSTTNDTFHAFKKYVDSIIDILIDDDKKSLEKYVDLYGKQEILFFGPDEGTAPFVDWATLHAKKRNCPWWKSFLTGKSPKLGGIPHDEFGMTTLGVRSFVENIYDEEKLWDSKLFKLQTGGCDGDLGSNEVLLSSKNETYVAIIDGNGTLVDPQGLNIEELRRMAHARLTTEHYDQSLLSSSGFYIPCDVQDFKIPSTGEIIPNGTIFRNKFHSEYLFKYLPKIDIFVPCGGRPGSINLSNLHYYIDPKTNKCRIPYIVEGANLFITQDAKIKLEEHGCMLFKDASANKGGVTSSSMEVLASLALKDDDFVGKFLQNVNGCYDNYVKFIQKKIVHNAKLEFKQLYKINKQTKKPISELSNTLSAAINKLNDDLIDSDELFKNDLPFKQFLLIHKIIPSILFDISSQSHLEVLDNIPENYLKAMLSDYLASNYVYQYGIDVNMGKFLEFIGGLKREAAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNL1</accession>
    <name>A0A1E5RNL1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GTP-binding protein RHO3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g815</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNL1"/>
    <dbReference type="FunCoup" id="A0A1E5RNL1">
        <property type="interactions" value="137"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNL1"/>
    <dbReference type="InParanoid" id="A0A1E5RNL1"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0488">Methylation</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="153"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="268"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="157"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="174"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ88492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="294" mass="31936" checksum="22A10540A7B75D3E" modified="2017-01-18" version="1">MPLCGSSSSTTNQHSSSGVKKSSNKPVERKLVILGDGACGKTSLLNVFTRGYFPKVYEPTVFENYIHDIFVDNQHITLSLWDTAGQEEFDRLRSLSYNDTHVIMLCFSVDSRDSLDNVENKWCGEIADFCEGVKLVLVALKCDLRNENQAPRMVTPATISKQQETGLQQDDTDPNNGLNSNGNNNNNNGNNLNNNINHSISNGPGSANNNRASNMITYEEGLAMAKKIGALRYLECSAKLNKGVNEAFTEAARVALTARAIGAEDHVERVKNSESSVANGSSSSSSSSSKCVIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNM8</accession>
    <name>A0A1E5RNM8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g816</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNM8"/>
    <dbReference type="InParanoid" id="A0A1E5RNM8"/>
    <dbReference type="OrthoDB" id="4065319at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051009">
        <property type="entry name" value="PRM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36089">
        <property type="entry name" value="CHITIN SYNTHASE 3 COMPLEX PROTEIN CSI2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36089:SF1">
        <property type="entry name" value="CHITIN SYNTHASE 3 COMPLEX PROTEIN CSI2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="222" mass="24992" checksum="5CF05374AC9A9E30" modified="2017-01-18" version="1">MNTITLPEEQNIAINISGLFRLFKAKKMDSSNTKTRDFMYPLITPPLPDGNPYIWKSKKPEGFVFIIVGLFAVLVFSLLFIWYAIQGFLSIKAAKSTVHHYENEIFEEIDSFASTSSSSKIHTNSTTDAILPVLSAKDGNPGNEKTNGSYNEPFFIQEQPIRNAFLPDSSVEEKNTIEKLDPVYITSLRSAGKTKRKPTPSMHLDALLYGSPKQIFDFKNED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNX5</accession>
    <name>A0A1E5RNX5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PH domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNX5"/>
    <dbReference type="FunCoup" id="A0A1E5RNX5">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNX5"/>
    <dbReference type="InParanoid" id="A0A1E5RNX5"/>
    <dbReference type="OrthoDB" id="3972951at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37283">
        <property type="entry name" value="PH DOMAIN-CONTAINING PROTEIN YHR131C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37283:SF1">
        <property type="entry name" value="PH DOMAIN-CONTAINING PROTEIN YHR131C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="448"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="624"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="673"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="717"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="851"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="887"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="929"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="236"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="457"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="489"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="630"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="717"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="854"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="890"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="941"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="960"/>
            <end position="969"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ88577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1238" mass="138575" checksum="6479D6A59FEAF784" modified="2017-01-18" version="1">MLGLKKKSLVSSPSLNDQESLKSQQSRGADITSVNNDRGGANGTSKVAREHTGSRSMSNRSDSNRSLSHRSLSRRSLNARNHNNANDICNDSALVQSPTLLPSERHLELYQWLDPLPAYPPSYSKCNPQNYSNVAFPIYELPHDPRVNNDMPPFYEPAIESVTVVLLETEYLSPFDKTTLKSWKHYIMELNSTQLNFYHLDHELTKNIPYYTNGKDDICELREQRERLKTIDSNKKFTSSNSSSSSSKTNKNNLSNNGSSSGSKESRFQSFASFIGSNKNKNNARTNISNTKDKNRSRSNSKNALSDAPQDNALNGLLQGFQSKKTLQLTKQDTKLITQQILNEPWKYMTNEKLCKSYTLQHGEFGIAADVVEKPFCIRIRCEMEQLLISFSSVMDMINWSVYLSTAIGISLDIDERELPDYKVVPVGTGRSRRRRLRRRLRRRNRRKRLEEEEEQQQQQAELEVQNQLAAATQSPPLQPGDENKQSHTNDNPGKSTKLSVLSRPNTKIIPRNVVFINNTTISNKSSNGRSNDFDELDETNDLGHEISREVTHQEDHQRAEERETRRIMEEYTSATLKRFVNDQDESDNDDTNSDFKNNLTYVDTRSRSSSVCTADYPNISRMNTNNSGNTVSSAATRTTSNTNSNYQQHPLRLIHTTSSSMVASKKNMDTLRARRVSSSSTSSKGPSQVKLEQMKPRSKTFSNPVAKLWSKKFSTNDTMQNNNKTKPSKLTPISSSSFSQDLPVSKNIHFTSATVSSNSTQLLTKSALGQPAPLPALGNTLTLHNDNAKSITIDDVVNASPSIRRPSFTARRNSLNASPNSVANSAMNSLSCSTFSSNLNNTVLTTTTPLSSVENSALENQSNDGEKLNLLDNEDPLEEFPSFAREGKASTQDAQTGNTNHALSSKGQSSNAEITTVNKPGIFAHSYNNNSGELANESAVIEEEEEEEEEEEEEVLVNDNVSDPNSQLSDDEMDRQLSETLSALSTVDSCNDFETIGHQNSNSMARRFKQPRAITEILSACRTDSTGQHLDNIQTVSTMESYPEEEADEAERELMEDMENDDEADTSSGGNPALAVYEEEGIHHDEEGFIYYQTGPVRRDSSTNNDFRRRAYSVASNLGSIPYGSQDIKWNPPRKIMTKKKYIREAIGCIKPLLQTQPWYGKVVCTPSAEPQFPTNNPAISGILVEAENVLEKKTSFFKTSSSQNEAKQPRQRVILRKCKNHYVRQSVIGPAGFIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNZ5</accession>
    <name>A0A1E5RNZ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">uracil phosphoribosyltransferase</fullName>
            <ecNumber evidence="4">2.4.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g757</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPRTase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.9" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNZ5"/>
    <dbReference type="FunCoup" id="A0A1E5RNZ5">
        <property type="interactions" value="736"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNZ5"/>
    <dbReference type="InParanoid" id="A0A1E5RNZ5"/>
    <dbReference type="OrthoDB" id="106623at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004845">
        <property type="term" value="F:uracil phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008655">
        <property type="term" value="P:pyrimidine-containing compound salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000023">
        <property type="entry name" value="Probable uracil phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001097">
        <property type="entry name" value="PRK00129.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="6 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="10">
        <location>
            <begin position="16"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ88599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="219" mass="24622" checksum="336F6C74931EB3B2" modified="2017-01-18" version="1">MLTGPTEPFKNVILLPQTNQLLALYTIIRDQNTKRTDFCFYSDRIIRLLVEEGLNHMPVEAKTITTPTGQTYDGVSFLGKICGVSIVRAGESMEQPLRDVCRSVRIGKILIQRNEETAEAELFYEKLPEDIHERNVFLLDPMVATGGSAIVATKVLLEKGVKQENIFFLNLVCCKEGIDLYHKEFPNIKIVTGAIDSGLDTKKYLNPGLGDFGDRYFCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RP15</accession>
    <name>A0A1E5RP15_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DNA mismatch repair protein MSH1, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g728</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RP15"/>
    <dbReference type="FunCoup" id="A0A1E5RP15">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RP15"/>
    <dbReference type="InParanoid" id="A0A1E5RP15"/>
    <dbReference type="OrthoDB" id="2534523at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043504">
        <property type="term" value="P:mitochondrial DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003810">
        <property type="entry name" value="PRK05399.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF34">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="DNA repair protein MutS, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="7">
        <location>
            <begin position="858"/>
            <end position="874"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ88624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="980" mass="112013" checksum="80470F4954D96BB5" modified="2017-01-18" version="1">MAFQLIAKGILGKQLERTTVCFFNQVLRCCSRPASTLSQKQSNSHEEIISLNLSFDQRTPLILQKDDLKPETIKNVSMPTVLQQVRDFIDKYENHVVLTQVGSFYELYFEQATLYAGKLNLTLTSKKYTCGVIPFAGFPSSQLSKYVKILVKDYGHSVVIVDQYKKPNVLDRLNESAVPSKDDEISSLKVVRRVSRIVTPGTLLDEAFENLASSTFLLNVEFPSTAFENLANLDMKLGISWTDISTGEIYVQRVYLKELVSAITRIKPSEILLDSRLAKYHIENGEWFPEFVELKKYFLSYYMKPSRHRTFDTFKHLFSINDHKRYHLFTNSVDTWEGAALRNLLTYVEEHLPECSTNLQIPERKCSSEIMQIDSRTSAALELHNTVRTNSVRGSLLSVMRRTVTTSGTRLLSQWISAPLLNVEEIRRRHNIVEFFSKNYSVTTAVIENYLKKIYDVTKILQKFSMNKGSYIELLQLSHSLKSTKSLKDFLLSKALTVNKDPQRFEEIIHDIQVDESLVNEVLESIEEDKIVTFMEKLEHSNAASSSFGKSERALSSSFSYQPKLNNRELFSQDWYIKPNASSKLKRLHKQFNSLVKEYDDLYASLQLKLVEKLGFKNFEFKISLSNGFVLQVNGPAIVWKRYSNFAKIAPELEFVQGKSTKKTFQFEPWTILGHKLERTIFQIREEEECIMENFKTRFIEDSYALRKTAAVLDYLDVLTSFAKLSVEKNLICPEIDDSKNIEIHQGKHIVVEDGLLNNMAKRFTANDCNLTGNKGTKTWIVTGPNMGGKSTFLRQNAIIIILAQIGCFVPCEHCKIGIVDKIFSRVGSADDLYNEMSTFMVEMIETNYILQGATERSLAILDEVGRGTNGREGVAIAYGTLKHLISKNKCKTLFATHFGKELQKMIEPNDRSLVGFYKTDLISTEDSDGEQENFRSSKYDHRLKPGICTKSDALKVAYEAGFPEYALNYAQEALKNIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RV90</accession>
    <name>A0A1E5RV90_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mitochondrial intermembrane space cysteine motif-containing protein MIX14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g333</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RV90"/>
    <dbReference type="FunCoup" id="A0A1E5RV90">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RV90"/>
    <dbReference type="InParanoid" id="A0A1E5RV90"/>
    <dbReference type="OrthoDB" id="276296at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2900">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052848">
        <property type="entry name" value="CHCH_domain-containing_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031731">
        <property type="entry name" value="CX9C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47106">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47106:SF1">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16860">
        <property type="entry name" value="CX9C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMS import disulfide relay-system CHCH-CHCH-like Cx9C" evidence="1">
        <location>
            <begin position="13"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="113" mass="13013" checksum="4FDE302D932BE6C8" modified="2017-01-18" version="1">MNTHDSVLDQIIMEDVSFNCPQQFMAYHKCISENHEDPSKCAFRQQDLSNCIKTSVPSVMKIMKTCGPIMQKYETCLRDNMESRTINENCIGLLNEMRQCAMNQIDVSKRSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVB0</accession>
    <name>A0A1E5RVB0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exocyst complex component SEC5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g297</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the exocyst complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the SEC5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVB0">
        <property type="interactions" value="338"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVB0"/>
    <dbReference type="InParanoid" id="A0A1E5RVB0"/>
    <dbReference type="OrthoDB" id="26242at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029175">
        <property type="entry name" value="EXOC2/Sec5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039481">
        <property type="entry name" value="EXOC2/Sec5_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13043:SF1">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13043">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15469">
        <property type="entry name" value="Sec5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component EXOC2/Sec5 N-terminal" evidence="7">
        <location>
            <begin position="93"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="59"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="665"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU365069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF15469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ90890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="910" mass="103486" checksum="60A4230880B0224A" modified="2017-01-18" version="1">MSAFFKNEQNLLDVYGLASSNPKGKWTECSTFANLSKPLIGFETDGSKMTTEQAHDILNELGRLEKKKKSGSKTGKGRSTEMNTDSPPFVDIDPLDGNQISNRVPNMSDPLLYSINSTKFNPKLFLQNVHSNDSFEQFTKYLDTLDSDLNYQSSDLKKFVHSNFTRYVRSKNNLDNIYEQFNAGSAQLNGGSASANPQTKTTTREDEKFQSLEKSVLDSSKKGIVLSKKLMMSDDLIKNYVKAISYIENSANLVNLPRKLRALLNNGNYSELLYEYNDAKKTYCSKXPGPNERKIWNEVENVVDQYRQHLWNLLLENDTGSGFIESKYDFLPIISKLLDLDVEDSPICLWIDAKLDQAKNKLKSVSSLMTSKLEEKKLSISLQNLALNSSSSSDPGVLPEKLSYYLQFTTQDSFSETQFVDQTVVVEMWFLIIXYINDVHQLATSFVEFWGNVQNFLNGSFQQTLINDKKRENIIFGNSNTSNGMKFASDLEPYQIGNIIEKGTEFVNDLIILLQNLFEVNSEKPTEFNFLVPGCNNLSCLRYLPMVVEPVFRLCNDIGQLXIDSTVNDSLQKACSQILENSIAAIDYTRLADYKQFDSLETWSIYEFNENVGQTGKCTFLPIIVKNVSIATINTLQNLVFFYEILPVSVTNFTALGKPSAAIIKHLQEEQVKALETVLESLLKDANRKKETPRTMETLLTLNNIQVLAKDAFPFILNYFQTQFDISSKNASAFSNLQMFTILEKMHSSILINYASDLKYELRQTLEKGFTSIDWISYTTTSFRASDYILEVLMILVQVHRDCSNISPNMSQKITSEAQQFISTYLYKLFKEHIDGVSTDGLLQVTLDFEFFIKVLSNILDAESERKLKSCLLYCFHNNPQRLEECVQQIQGAIDSSMNRTAIQFASFRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVQ8</accession>
    <name>A0A1E5RVQ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">5-hydroxyisourate hydrolase</fullName>
            <shortName evidence="7">HIU hydrolase</shortName>
            <shortName evidence="7">HIUHase</shortName>
            <ecNumber evidence="7">3.5.2.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g436</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the hydrolysis of 5-hydroxyisourate (HIU) to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>5-hydroxyisourate + H2O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23736"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
            <dbReference type="ChEBI" id="CHEBI:58639"/>
            <dbReference type="EC" id="3.5.2.17"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4 7">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the transthyretin family. 5-hydroxyisourate hydrolase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.17" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ91021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVQ8"/>
    <dbReference type="InParanoid" id="A0A1E5RVQ8"/>
    <dbReference type="OrthoDB" id="10265230at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033971">
        <property type="term" value="F:hydroxyisourate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05822">
        <property type="entry name" value="TLP_HIUase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.180">
        <property type="entry name" value="Transthyretin/hydroxyisourate hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014306">
        <property type="entry name" value="Hydroxyisourate_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023416">
        <property type="entry name" value="Transthyretin/HIU_hydrolase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036817">
        <property type="entry name" value="Transthyretin/HIU_hydrolase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02962">
        <property type="entry name" value="hdxy_isourate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10395:SF7">
        <property type="entry name" value="5-HYDROXYISOURATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10395">
        <property type="entry name" value="URICASE AND TRANSTHYRETIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00576">
        <property type="entry name" value="Transthyretin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49472">
        <property type="entry name" value="Transthyretin (synonym: prealbumin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transthyretin/hydroxyisourate hydrolase" evidence="8">
        <location>
            <begin position="4"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ91021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="150" mass="17199" checksum="E9110E728FF4A454" modified="2017-01-18" version="1">MPPITCHILDSSVGKPASNVLCCLYKVFPEKNDTETLFAMNNTNGDGRISSWIVNPNLGIDWLRANISSSACSFKXXXNTEGKSSKSEWQSGITCGQYKIRFYTKKYFDAKQEKTFYPFVDIYFHIENNTEHYHIPLLLTNYSYTTYRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVR3</accession>
    <name>A0A1E5RVR3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g313</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVR3"/>
    <dbReference type="InParanoid" id="A0A1E5RVR3"/>
    <dbReference type="OrthoDB" id="3026777at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507:SF1">
        <property type="entry name" value="FI18259P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507">
        <property type="entry name" value="ZGC:174356"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="339"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="377"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="419"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="479"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="508"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ90926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="547" mass="61285" checksum="318767915C1D8BD6" modified="2017-01-18" version="1">MTIPKSSTESCGLKPNMDIVSDYSSLTSPENEIAILEEQDTERTRFRSLFSSAKYKSVAKKLSFNIKPNVYMLIVLLAIRSVGETISLAPSVELTKQKIINAFPDSAESGEKEFLRIQVINGLITCIVGMVVCSKYGIASDHHGRVFVMKICAFFTCVRVLIDLYLYSNHFKYSFFMFNFWKSIGLLDGEAMAVNILSASYVSDIVSKKDRLVYLSLIGSASAGISVVSPLVSSSVVAAFGDYQVLYLTAASYATFFLGVTIFLTESSSERSRTLSKSEHESRIAKRKAKRETTQHARSLWIKTYHILRLDFVLDVLHPLRFFYLSKTSTGSLVPRHNVLILLLIETFVLGTLIGASPVMVSYLLLSIGWGSVELNYYISMTSAVKLFSVVLIPRYFYKFLEQKCGFKQFTWSIDKIDLLNMNILALGVGFALIIPLCLVNGGTGFYLNGIIISLIQFASPTIQNSVLKYCDVKNSGQVFTAFVLLSQMFAIVIPPVFFWIYSKTLNSWPTFFLVIPVITCCISFVMLKFVKIVDDPKILHLSDEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZD3</accession>
    <name>A0A1E5RZD3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UBX domain-containing protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g30</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZD3">
        <property type="interactions" value="115"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZD3"/>
    <dbReference type="InParanoid" id="A0A1E5RZD3"/>
    <dbReference type="OrthoDB" id="3972947at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46424">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46424:SF1">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23187">
        <property type="entry name" value="UBX7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="2">
        <location>
            <begin position="289"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="431"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="436"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="456"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ92311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="485" mass="54980" checksum="DA1588C32EF00CD3" modified="2017-01-18" version="1">MSDLYYLFQRSIESAITKAQTEQKPLIIYTVGSDNDDLWLTTWLNEGNIENLKASGVLLKLIKDTQQFKFFSDMIPNVVTPALYCMFQNNLIGVIHGETEDLSSFSTKLEEWLSMYNGSDETPPVMNVANETNSSTLNTAPEVAVPXANSNVSSENTTTTSGSFSYPEAQNYSAVPSTHNAPVYELANHQSASDQELASINESXDQKNKLSDHRVKMSESEKKYREELLQQQKKAKEEKLRITHLLEQDKKERQALLREQRAGFKNSFDDKSNLGTKQTLKDNISQEKQLTNKCALAFKLTDGKMLKNIFDSYLTLNDVRKWVDKHRTDGDTPFSFHRNIPRMTFGDADELRSLKDLELPPRSVLILKTXEFDHPRKLVHASNASAQGLLSKMYSGMATWWGXSVNHYEEQTSANQNDELCQEQVDENGGVENXVSLSPTMSSKSXSXTPNVHQLLLDKDKEDEPRETYNGNNLSLEDEKKKKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZH0</accession>
    <name>A0A1E5RZH0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g207</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RZH0"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000372">
        <property type="term" value="P:Group I intron splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025694">
        <property type="entry name" value="MNE1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13762">
        <property type="entry name" value="MNE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ92357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="636" mass="74074" checksum="28A12D628D29F6FC" modified="2017-01-18" version="1">MFQYFFRRTIVTQDKVSPVRKIILSCLPDVRKTMLPSRKSTLIPYQADDPCDSLFRMSMKXGTGPEQVPVIKSPLFLVNLRGFIEESANSRHHGAFSANIVALLHRLENFSYRWTGSFKASLGRSNKFFNLKFKETSHSSICTVVPVEVLDQIVFEISEKQRALLRFSKXNGNTEXVTNSKRAARMLFSLVEKNYKDKELPLSFWKKLCNLACSVDEVELIVKLTNDSSLQTIKQDYIEMAIYRTKKDWLKFVAKFHQLEKSFLDEIVYNELFGSLYFDSLERLMKAGIHEKDCIDIYNRCLKRLGLQGQFKLLHVSETHGLTKMNAHIQASLSSLTGYLKDYSESIQDYLHELQNMNVELDFCKNPDLLNELEFLTVKFPTFEKASSYCSFTDQVFAQVTNPTLRLLYAHFLLKNISSTKSFVDFSFIIKDISKRLFSTERHFPKLSDFNVLSSLVNSQNDVFYSIFENLSRNRDCQLTTYRIYQYLKKNMVRDFTAKDYYCLIKANLQGDSFLAAYYYIGELILNLGASFLDDNKKWSLPHKIEKDLIEKGLINFFGDTEIESVLYQVGKQFEKVQRPISREELLRIMYVPDVIDSLSMKEFVFQGKACARMLPWYHEQRDMNKLESVAYLIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZR4</accession>
    <name>A0A1E5RZR4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">54S ribosomal protein L27, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g57</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL41 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZR4"/>
    <dbReference type="FunCoup" id="A0A1E5RZR4">
        <property type="interactions" value="157"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZR4"/>
    <dbReference type="InParanoid" id="A0A1E5RZR4"/>
    <dbReference type="OrthoDB" id="408933at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019189">
        <property type="entry name" value="Ribosomal_mL41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21338:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21338">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN L41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09809">
        <property type="entry name" value="MRP-L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ92441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="152" mass="17758" checksum="887AF92422262762" modified="2017-01-18" version="1">MFKPSNILQAAGFRFNRAGVEVWSTHVRRHKNVRLPTTTKDGRNSFYKGTRSSGIGRHTKFGRYVINWDRVRTFVTPEVPNLHLKPLVSHHVDELKNKYDAELYPKGAQDGQLYFDSLIKYIKEGETPVSLDMQKKRVFNYSKDWMKPKNSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZU3</accession>
    <name>A0A1E5RZU3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ubiquitin-conjugating enzyme E2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g74</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZU3"/>
    <dbReference type="InParanoid" id="A0A1E5RZU3"/>
    <dbReference type="OrthoDB" id="269518at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="6">
        <location>
            <begin position="13"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="301"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="307"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="3">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ92487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="338" mass="38323" checksum="4EDB6EC8EBF21EE2" modified="2017-01-18" version="1">MAGYGQKSERFTSTAGSIKKIEDDLKKLYKCGRYKVFLDDENIYAMSIQFKGPKDTPYENGYWRLNLEVSNEYPFKSPSVGFANRIYHPNIDFESGTICLDVLNSRWTPMYGLKDIVEWMIPGLLKEPNASDPLNSAAARLKAADEKEYERKVRQYVVEYASKTAYNNKFYPNGVCNDLEDHLYYVSEVEEEDGEENGKDKDKTYKEXNIAKNGPLNKKSFKYTNTNSNGSFDSFGIDQQVATINGTNKTATTTTTIAVQNSNGYSALLEKTMHTLSDNMNKLDQYHHTHAHSEGKEFFINGGCDSSMDDNDNDDDDDDDDSLSDIDISDTDLMECEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S022</accession>
    <name>A0A1E5S022_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chromosome instability protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g206</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5S022"/>
    <dbReference type="FunCoup" id="A0A1E5S022">
        <property type="interactions" value="190"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S022"/>
    <dbReference type="InParanoid" id="A0A1E5S022"/>
    <dbReference type="OrthoDB" id="10253476at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048487">
        <property type="term" value="F:beta-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007023">
        <property type="term" value="P:post-chaperonin tubulin folding pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058033">
        <property type="entry name" value="ARM_TBCD_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033162">
        <property type="entry name" value="TBCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12658">
        <property type="entry name" value="BETA-TUBULIN COFACTOR D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12658:SF0">
        <property type="entry name" value="TUBULIN-SPECIFIC CHAPERONE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25767">
        <property type="entry name" value="ARM_TBCD_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin-folding cofactor D ARM repeats" evidence="1">
        <location>
            <begin position="384"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="959" mass="112350" checksum="F9C986AD186DE85C" modified="2017-01-18" version="1">MDQDVAKPQMYRPTLQARVRMNHASHLSNIALIQDGLPVVQETLANILISIEYFQQFPDYLVKKMGSLRVFHSSIWNIEQPETPLSRGLPFHRPNPNLIIAAQLFHKLSVVIKYKKLIQYLPNDLELIPVLIDLIVYYQDAPTEPLVFKEQEKWLVLNYLCHYLLLNVMNPFNLSQKISETIITTIISLDKRFTIACNPFLVATLLDRNSFLLTKYYQHINYKTINQFLKANEHNLKQVFSLPEIICKDWKYLIYTENISNTVERVDHLSLLKIVPRLAKYYLCSDQPYLKELLQWYMTIFSGTNEMSSEVLGNTEHRFKLAKSFVLFLDNCHMHTIERLYDYMLFPEASDNLEILHLKLLCIAFMIQNQSFDHDTQIDMSTLKVVPIISQYFDFQSLNYTNYHDSAYNKIRDAVNFITWAWIRSKNKITSAQLVPYTKEIFIKLLFSSLFDYDLNIRRSALATLHELLGRFAVLDNVTTVELMTILNVINQHHIYSVVIPQLFDLFKKHGYQTYNMHLLERMILINVGKLKKINITKQTVFVLSILYENQEWHEQINEISKKIVLDRQNTIEYLYYYTSIELPPPVFDVETCYNSFKTLSVTPGSVETSFRYLTLLKSLNYLLKSSTKKSLSERDIDHVYNIFLYSNKIFNNKTDYAEFYKELAILINTLEDTKTFIYESTSSHDHWQKVFVKLLSSNNNMLFQLLPSMDQIYENSATLTGYLRKCNHLTKATLINALADCFSKLVCCNPEAIDVLLLSLDDYTSTQEGDVGRKVRESGLNLIKNNMVYFRQKKYLITPKLLKLACEPIESLQKLSFSILLELYEKPAQEQNPANFYECVIDFYSRHGNEHRSSFFNGYVATAGAIHCPDFQITDSLNAFLDYFFLLHEQDKLQVLNDLVRIFPTAETIKKCRPKENFNEINSPQKDLIKHTLTIFKFWDSLDIFERLDLLQYFTTNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S027</accession>
    <name>A0A1E5S027_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Guanosine-diphosphatase</fullName>
            <ecNumber evidence="11">3.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g201</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">After transfer of sugars to endogenous macromolecular acceptors, the enzyme converts nucleoside diphosphates to nucleoside monophosphates which in turn exit the Golgi lumen in a coupled antiporter reaction, allowing entry of additional nucleotide sugar from the cytosol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GDP + H2O = GMP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22156"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.1.42"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the GDA1/CD39 NTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.42" evidence="11"/>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5S027">
        <property type="interactions" value="366"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S027"/>
    <dbReference type="InParanoid" id="A0A1E5S027"/>
    <dbReference type="OrthoDB" id="6372431at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004382">
        <property type="term" value="F:GDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045134">
        <property type="term" value="F:UDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009134">
        <property type="term" value="P:nucleoside diphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24040">
        <property type="entry name" value="ASKHA_NBD_GDA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.150:FF:000009">
        <property type="entry name" value="Guanosine-diphosphatase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000407">
        <property type="entry name" value="GDA1_CD39_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782">
        <property type="entry name" value="ADENOSINE/GUANOSINE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782:SF83">
        <property type="entry name" value="GUANOSINE-DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01150">
        <property type="entry name" value="GDA1_CD39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01238">
        <property type="entry name" value="GDA1_CD39_NTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="263"/>
            <end position="267"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ92355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="535" mass="58368" checksum="7A6C2B6EAF1079CD" modified="2017-01-18" version="1">MAQHIFRSYRFIIIAVTAIMLILIIKTSTDHHTSATENKHYTADDVNINLAGQSNDDIPSGKVAIIEDSSKVVESPTTSAAALSAXXSADGKKTSSASSEVCDKDHQYIVIVDAGSTGSRVHVYEFDVCTSPPSLXHETFDMLKPGLSSYPDDANGAALSLNPLLDLAVETIPKKFRDCSPIAVKATAGLRLLGADLSTAILKEVRSHLETNYPFPIVDDKDAISIMGGDDEGVYAWITTNYLLGNIGAPERLPTAAVFDLGGGSTQIVFEPTFANKNEKMIDGEHKYELEFGSEKYTLYQFSHLGYGLMQGRNKINSLLINTSLKNEVVTADELATGNVELVSPCLPPNTTFSDKVKMEDGTSYSVTFKGPKTPAGPQCRYLADKILNKDAVCESKPCSFNGVHQPSLVKTFNEHNDLYVFSYFYDKTQPLGMPLSFSLNELKDLARMVCNGEEVWQSVLSGVEGGVEELQKAPEYCLDLSFQVSLLHTGYDIPLHRELKTAQTLAGNEIGWCLGNALSVLDGNNWKCKVNQLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S0B4</accession>
    <name>A0A1E5S0B4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">GPI ethanolamine phosphate transferase 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g42</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the GPI second mannose.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the PIGG/PIGN/PIGO family. PIGG subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5S0B4">
        <property type="interactions" value="478"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S0B4"/>
    <dbReference type="InParanoid" id="A0A1E5S0B4"/>
    <dbReference type="OrthoDB" id="272139at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051267">
        <property type="term" value="F:CP2 mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16024">
        <property type="entry name" value="GPI_EPT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037674">
        <property type="entry name" value="PIG-G_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039527">
        <property type="entry name" value="PIGG/GPI7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045687">
        <property type="entry name" value="PIGG/GPI7_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013870">
        <property type="entry name" value="Ribosomal_mL54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23072:SF0">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23072">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19316">
        <property type="entry name" value="PIGO_PIGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08561">
        <property type="entry name" value="Ribosomal_L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 12" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="422"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="449"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="476"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="504"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="542"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="567"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="619"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="659"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="701"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="736"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="771"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="domain" description="GPI ethanolamine phosphate transferase 2 C-terminal" evidence="13">
        <location>
            <begin position="417"/>
            <end position="820"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF19316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ92458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="908" mass="103551" checksum="56440AE5EDF021BD" modified="2017-01-18" version="1">MRQVWYFCLTVFQLIAVFIFAIGYFPSKTLLKGHAVPYTSNDGDSGQQVQDSKHGGSVPQFNKLVFVVIDALRSDFLYHQEVSHFHNLHKLINDGNAIGFTAYSNPPTVTLPRLKGITTGSTPIFLDAILNVAEDDSSSNLQDQDSIIWQFYHSKNMNKIKFYGDDTWLKLFGENTYFQDHDGTNSFFVSDFVEVDHNVTRHIPHNMAHKEEWDVLILHYLGLDHIGHKGGAHSLHMKPKHLEMDQIIKNLYDSVDEDTLIVVLGDHGMNDLGNHGGSSSGETSSGLCFISKKFGSNGIMDNASKQKVPVVEKNLDYQYLNKATQIDLVPTLMALFGLPIPINNVGIIIPELLPWINDKEFHINENHKQLDKLLPERFFANGNANLEKNHSQNIMSLEELYRDMHEIQEKLLKKSTEYNYKFIAYGFGLLILSNLLTFYCTFSTNIDIYYVIVVGISVLFGISAFGSSFIEEEHQIWWWITVLFYCGSFILTKTDNNNNIQRKSNSMVXVKFILGLLCLRFIRGWNNSGQKFQYDKVMAKLISPNVLWVLNFMAILLPCWKFAINGKWGSFVAVSIGALSCFTYKLNWSVVNEHLEXSSQGFFFKFLQKLSSNEELVDYAQFVFKYTGVCLIGTKAISWFRRNTNGTRKETRSPVSTQLILVSLVLILQSAPSNIPLFGLFHVLNYSVNDFIFDDTRKASIWFLLMEHLTFFQFGGTNSINTINLTNAYNGISNNYNIYVIGLLMNIANFSPSIFWALLGSHHPEYKRNTLIFYNIFGICLLASCFVSRYHLFVWSVFSPKLCYYIGWSLFMNFICSASLQNSTPTKTVVSSRKAGTPLNLKIKKTGNEPVALEDSEYPAWLWSVLEEKVPETIPSNISKEEQFALRKKQLRSANRQKIKSNNFLGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S0D3</accession>
    <name>A0A1E5S0D3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g103</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5S0D3"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ92478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="160" mass="18209" checksum="ED5572D10BC7C48D" modified="2017-01-18" version="1">MGYSTQYSSKDXEQQLETTTNCVPIAMSIPNQEGQLQVIEIESQIISKYFQLVDEFGKFNRDILSGVNKILVESVDRREAQXXXXXEDQSXXXXEXQSGETQEDQSGAVESVIQPDNELGIMDPLSLQLKYLEYLIENTDPKESLMVLDKIEQYLRAKEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0B8</accession>
    <name>A0A1E5R0B8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ATP synthase subunit K, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4473</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0B8"/>
    <dbReference type="FunCoup" id="A0A1E5R0B8">
        <property type="interactions" value="93"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0B8"/>
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    <dbReference type="OrthoDB" id="2094445at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="ATP19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28074">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT K, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28074:SF1">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT K, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11022">
        <property type="entry name" value="ATP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ80327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="69" mass="7604" checksum="EA6CE8A5C2DED989" modified="2017-01-18" version="1">MGAAYNILGRSVLPHQLALGTFGAVIFFLLPNPFASKPQVQSVPIKAGSKDEEDFIKKYLQEHSTTEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0C6</accession>
    <name>A0A1E5R0C6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Very-long-chain enoyl-CoA reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g4458</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the steroid 5-alpha reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0C6"/>
    <dbReference type="FunCoup" id="A0A1E5R0C6">
        <property type="interactions" value="409"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0C6"/>
    <dbReference type="InParanoid" id="A0A1E5R0C6"/>
    <dbReference type="OrthoDB" id="540503at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039357">
        <property type="entry name" value="SRD5A/TECR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556:SF28">
        <property type="entry name" value="VERY-LONG-CHAIN ENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="264"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="10">
        <location>
            <begin position="155"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ80358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="306" mass="35264" checksum="5FF17BDB5C6EA2B2" modified="2017-01-18" version="1">MTAITIKSRSKTLKSCQIETTDADNLASVAQKISAQNKKINPNRLRLTYLKESKQIPLEKFDFALAAADGMDLYVKDLGPQISWRLVFVIEYFGPILIHALVYLASLNPSLVAKYHSSSQNYNAELSKLAFMLNLLHYLKREFETVKIHKFSNSTMPLFNVFKNSFHYWVLNGLISLGYFGYGFVFPESILTKVYSHLQIVPLIGAFLFFETMNFKIHYDLRKFGDESAKKGVTKRVPLQHGLFKIFVAPNYTFEVLSWLTFTVIFKFNLFALIFFSVSTTQMYLWAMKKNKKYGTKRAFLIPGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0F3</accession>
    <name>A0A1E5R0F3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Regulator of rDNA transcription protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4551</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0F3">
        <property type="interactions" value="177"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0F3"/>
    <dbReference type="InParanoid" id="A0A1E5R0F3"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10352">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="58"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="154"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="380"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="201"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="216"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="232"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="5">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ80375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="408" mass="45846" checksum="E42088EA55982019" modified="2017-01-18" version="1">MSELQEVAETTHDPQQDKDNTVYNNIGTPVAGGESTATIISNLQETGTNDSNPVFKXNKIYVSNVPYDATEDDLFQLFAPFGCYNVLIPAQTVRGFRRKAYSRSFGIAYADFETPEKAKVCVEKLNNTVFQHRNLVLKLYEPFIPQCRKQNYSGNAGKHGMGNKGHXQPINVSDLANDEEGENGLQKTTKPGKXXRKHRSMXKERKNNGAVKKDPSSNQGESNESISGETAQNERTENSLASKKEVSETTVYITNLDRKVTEVELKILFQEYGPIDIYTFYKKYHSRGLHIRIWQYAALVEFGESFKDPYITDNDYQIDRVHDNSNDTTGSLECSTIPRTTNKAIEASKKFNNQKLKGKNICVRPAYMSKIREIQNANVQDKTKVSNENEVARGPESEHVAGESSNGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0H5</accession>
    <name>A0A1E5R0H5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative mitochondrial translation system component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4531</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0H5">
        <property type="interactions" value="141"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0H5"/>
    <dbReference type="InParanoid" id="A0A1E5R0H5"/>
    <dbReference type="OrthoDB" id="10249045at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005740">
        <property type="term" value="C:mitochondrial envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000964">
        <property type="term" value="P:mitochondrial RNA 5'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013943">
        <property type="entry name" value="Pet127"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31014">
        <property type="entry name" value="MITOCHONDRIAL TRANSLATION SYSTEM COMPONENT PET127-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31014:SF0">
        <property type="entry name" value="MITOCHONDRIAL TRANSLATION SYSTEM COMPONENT PET127-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08634">
        <property type="entry name" value="Pet127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="859" mass="98694" checksum="1ED8A6D765B40494" modified="2017-01-18" version="1">MLKSKNHGRTLIKXVKCANRHDWREYSNFINNKEELVGKETAQPTNEHISHTGNKTDLDKTKSLEAALARSKVFAKTVNKTAATKDAVLFLEKPILKSSSQRNIERGKPRNKYLDGHSGAGGKLSRNRRLPQTFDKVNNNMLNKEKDKQLKTPKISKEKQTLNKPLRTPVFIEEIQKSVLDVETTQITDKKPFSTVNLVLQESENFQLSHIDKKDAVLPPTLKHGIDYALFQPLTFQLVKDKRSGKSVLGESVSTPINVHNFNYDALPKYKTPSEDKVLLQVAKDAAAKFYSSSSSMTGALSQIHFFLSNFRPVKHHYLSKTFETFSDYAMSAKQAVSLLVQRKSDDQSVTSAPVYSISNDKSADTEIILSQLGHALETQLTTTKEEFDKNYDKSNPGYKGTQASTKVTAQDYFHYSKISNFVLRSQLDSHHPDLPGSGIFDLKTRAVSAIRHDLAYVQKNNNFTGYALNKVYGTYESFEREFYDLSKLTLLKYGLQAKIGNMDGIFLAYHNISKIFGFQYLPLEDIQYILSSYHCDSFKGDLQKRKELLKVXYTPAELVSKELLPEFLDKRISKAIMDQEYKFSFQILNKMLSLIHSELNNCSNFRLMIKAEPPVYNKNEAIDVYRDEHQKTTLYFFAVPLASEDIRFLEERSLKTSLVDANPEKIDEFLKDLSHFNKTHFANKLVGFKLTITEQKVQGNGEKLPKVEFSNEEKSSNLNSREVKKLEQFTNGEGIYKNFDMWEHPYFFHPNDVKXWNINCKVEKINSPLTKAKMYSTYLEEKMRFVKSNIEEARLYENEELSKSKDVSVSKSKKVLTKKDIEKLKYLIGTQILPKNYRSSRQSYDDFKQTLRAYGQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0M8</accession>
    <name>A0A1E5R0M8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">HSP70 co-chaperone SNL1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g4546</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0M8"/>
    <dbReference type="STRING" id="56408.A0A1E5R0M8"/>
    <dbReference type="InParanoid" id="A0A1E5R0M8"/>
    <dbReference type="OrthoDB" id="417450at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.120">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036533">
        <property type="entry name" value="BAG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003103">
        <property type="entry name" value="BAG_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02179">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00264">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63491">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51035">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="BAG" evidence="3">
        <location>
            <begin position="113"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="91"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ80454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="202" mass="22890" checksum="E2BE80CA4567C678" modified="2017-01-18" version="1">MALTIGSTVTRPNFKTIMTSLVTNFKQFLDIDALRSFKTQFLSEQNNKYLPYIVAATCCCLVYFGIASNNSSNKNKKKKRPKKKTGKSKGRASSVQKSTPKTTPAPPKPKKTSAEEIQEVNVKFNKEYREGIVQLLESYDPAVEEDTYKKKFYGEMLLKLLIELDGVDLISMEEGEAKNALKTKRKQSIKEIQSYLRKVDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0Q3</accession>
    <name>A0A1E5R0Q3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Acyl-CoA dehydrogenase family member 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4425</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0Q3"/>
    <dbReference type="STRING" id="56408.A0A1E5R0Q3"/>
    <dbReference type="InParanoid" id="A0A1E5R0Q3"/>
    <dbReference type="OrthoDB" id="434771at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050741">
        <property type="entry name" value="Acyl-CoA_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083:SF13">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="6">
        <location>
            <begin position="370"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="546" mass="63127" checksum="F91F4F137F092AF1" modified="2017-01-18" version="1">MPIQLDTRPKSWTKPSLEENDPIINQFPFFIRDKVDEKTLKMCSVIRWFIIKEVIPKEAKYYDHGTTTAERNCIVQNLKDKAQHEYYIGHMIYGTRSKYAHDNDDLFINDYNTNTNGNPSSDTAEFNGKQPYSDSSFASTYAFKKPVFKLSPLEYGVLLQIMGVSLLSFQVIPIVNLQNKDFNSCTSINNMCAVNLIYRHGTKEQIEKYLCSDNFYNFKFLISEENCNSSDYLNTQFNIKLKKKELVLSGNKWFIPDVFNNNVQTGVTNNSTGFDTNQCKNNVHASNENGVNNTKNCWLLFGVTENTKDTSIHSKHSIVLLDEEMVHRDNLQIFQLFHDDVLNSSYYHLKFDKVSIPFDEKMTLLSTRGNGNNLLIEKTNLSKIYNSLLIIGLTQECVNRAISSSEDKIKITNKNTKLVKSDYFKNTLAEHKIDLENCKNSCYNALLKYQDFGRTNDCKMEVAMCKYMTPLKCCKIIDWCVQVHGGSSYLSCNSKLFQSYDYVRKFRVDEQPDEFLLSSIGKYEINHANKMFKTLKDLNFTDSFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0Z2</accession>
    <name>A0A1E5R0Z2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase</fullName>
            <ecNumber evidence="4 12">5.3.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">5-proFAR isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 12">Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4556</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:15469"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="5.3.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the HisA/HisF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.16" evidence="4 12"/>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R0Z2"/>
    <dbReference type="FunCoup" id="A0A1E5R0Z2">
        <property type="interactions" value="233"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0Z2"/>
    <dbReference type="InParanoid" id="A0A1E5R0Z2"/>
    <dbReference type="OrthoDB" id="446074at2759"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00009"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003949">
        <property type="term" value="F:1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04723">
        <property type="entry name" value="HisA_HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011858">
        <property type="entry name" value="His6/HISN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044524">
        <property type="entry name" value="Isoase_HisA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02129">
        <property type="entry name" value="hisA_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090">
        <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090:SF2">
        <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="8 12 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ80561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="289" mass="32341" checksum="6FB9CDA49AAFC92F" modified="2017-01-18" version="1">MTRFVGCIDIHGGQVKQIVGSTLANDGDEKAVSSSSNTEPQKTADLATNFVSTQPSSYYADLYINNNIESTHIIMLDGRKCPDTIKCAQEALSRKPNFFQVGGGMNDENCLQWLNEYKAAKVILTSYLFDSKTYEFQWDKVEQISKMCTPQRLVIDLSCKRSSNSGSPEWTVCMNKWQTLTNLSLNKEIFEKLNEYCDEFLIHAADVEGLCQGIDEELVAKLGEWCKDLKSDCKVIYAGGAKSLQDLQKVKILSGGKVDLTFGSSLDIFGGKLVKFDDCCQWNLENCKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R155</accession>
    <name>A0A1E5R155_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">General negative regulator of transcription subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g4304</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R155">
        <property type="interactions" value="1243"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R155"/>
    <dbReference type="InParanoid" id="A0A1E5R155"/>
    <dbReference type="OrthoDB" id="1933107at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20710">
        <property type="entry name" value="NOT1_connector"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.840">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 1 TTP binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007196">
        <property type="entry name" value="CCR4-Not_Not1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032191">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024557">
        <property type="entry name" value="CNOT1_dom_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032195">
        <property type="entry name" value="CNOT1_HEAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032193">
        <property type="entry name" value="CNOT1_TTP_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038535">
        <property type="entry name" value="CNOT1_TTP_bind_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040398">
        <property type="entry name" value="Not1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162:SF8">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16415">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16419">
        <property type="entry name" value="CNOT1_HEAT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16417">
        <property type="entry name" value="CNOT1_TTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12842">
        <property type="entry name" value="DUF3819"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04054">
        <property type="entry name" value="Not1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 HEAT repeat 1" evidence="11">
        <location>
            <begin position="159"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 TTP binding" evidence="10">
        <location>
            <begin position="599"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 CAF1-binding" evidence="9">
        <location>
            <begin position="784"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1" evidence="8">
        <location>
            <begin position="1095"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-Not complex component Not1 C-terminal" evidence="7">
        <location>
            <begin position="1730"/>
            <end position="2099"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1327"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1327"/>
            <end position="1337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ80639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="2129" mass="243518" checksum="E3F43E6B2CD17407" modified="2017-01-18" version="1">MSTSPEPPTTSKSLTEEDVYVLTYIGEIVTSIVXKNPDSAEVQAKIADIHNLISVASKEAFIRFFKKYFSLFQSNPQYQIDPAIKKLVDFELNIAINNPILIQILQDFFSKEEQINVLKTHYPEQLNKLESFYSFADQXKNIKKDTSMINLTNYQLLVKYLLKESTSTSFATSLDXFLGSFQGESLNEMIALLLSEIFCPGSQHFVPEISNWLTPVTDFEGSTQVGKQINEALVTLASQSSIGWSRVFNLMSTKYLIAADHVEPTLASLSSLFYCLNRGDLISQFFHCEWSTTLKLKLGLRLHDWNSSCFDLLSLDTITKVSSKFPNYGNSLLYLQPVSALILEIFFLRSELPPSKDLEEFTTRFFEDFNNFPENLLQCLMYTSKIFRLLVEDRAQIDDLMTSLAVQCFKKNFAFFDEIFYMIAAIFDQKFVKAVTESILVKYNSEIDQLAKSFISKNKFEFYVTEGIISFEDALQLFPSAFKYWSFDEQKTTVYKQFVKSKIQKSRTSVKILLNNLNNQINMDNNQKNSSSFSPLCYMDLDMLHFIFEELSSCSNLSPADFATFEDIKHNMVLKNPRLINFGYASSAKNEVLDLNDSFKPVSEEIEKGMQQCLQKMYSKEITIKVIIEMMQDLSSSKVKADQELFCYIIHGVISESPFYKDYPLDAVALNSVLLGSLINVHLIRGFVLDVVLRLILSFSTEPSDSKLFKFAVQALFDFKDRLVEFPDFCKRLLNSSFSLQTHPVVYDMVIKSVQEQEKQTLPKSTDVEVKYLVPVEPRTQIVQEAPSKDIQDKVLFNLNNISIENHQEKTKKLKEFLKPNYFEWFAITLINQRAKTEPNYHELYSKVIFLLNDPLLHDYVLFATYTQTQKLMSKGDISPQERNYVKNLGGWLGFLTLAKNQPIKNRNLAVRELLIESFNDARLEVIVPFVCKLLAHAASSVVFNLPNVWTNSIIELLVELNEKANWPLKLTFEVEVLLQVFNKKVNDFKASDLLSNPKTDEILSGKVFEESADMLQSENVLRNMIQQQTVXFLHNRMQQQQRAMGQNGVNNNLSQLNDRVNSLTLDQKQQXQQKPASDASFQNLMGNTIFVTHPDLRHLFHMAISKSVREILVQVVEKSSRIAVVTTTQLVLKDFATDADELKLKDTAVAMARQLAKKMAYVSCVEILRENIKNTTQSLCANIMNMIPQAAEELLVAVNENIGLALNLIENASMERANQEVLEVLMQPMAIRGYHKEKRAEENFVAPNVSPYAELLPEPLGLKAGGVTAQQLKIYEEFGQMGVASPSEDILHMQQQQQQQQQQQDSLLEQQKLKHRNVLLQQLRQDNGQQXQSQEQKGPPGLETIHGVTXNNTPMNVHAQPAMFNQPVIQPPPMNELKMQLDSCVHLIENLTQLVKENAEKKISDFGTENNPVTINVLRIISILARSKHKDSLALTLSQNCVSRLFQLSTVNLGIEAFTMLIDKICNISVVAKKDVAWWLVYAVDPRKLSVSCILSLIDCGLIQISSLDKMLTALLRSNLPHTVSFSIELISEILQKKDVSLIRTNFLESLIALKDINSQESKEALEKLLELQVMPVSSNTSITQRERLFLVFTEWVNLVQKADTDDFVVRCFIQQLIDRDVVDSTEKVLSFLDAGLEMSVESFKNSDPTSDVFTAIDALTKFVIQILVYQDFSFESREEFLRLLLIRFQLHLSTEHKKPNDYFNERPFFRFLSSLLFEWQKVRSHDFISISDQECRSELKEFDDSFYFVFSDFIHALQPLAYPSFAFAWVTLISHRMYLPTMLKMKDNKGWVKVNQTFVYLFRYFDKYSGIGQVPDSVAVLYKGVLRVALAIFNDXPEYFIENHFVLLNNLPSSYLQLRNLILSAIPKKMCFPNPYNRNIEISDIPECTNNNPSQLYNTSAELGPLKKPIESYLRIPSSSLMRNISNQISCASRLEEIXGFGFDITTIDRKLLNAVVMHIGEEAGNELLNTSKQAILNVKSSYYMLLSNLIANGKDEFIYYMIDCMVNQLRYPNAQTFFFHYTLTTMFKDGTSSWSNTKKNSVQEIILRALLERLLVHKPHPWGCVLTFLQLLNTENILDLDCVKNSEKVSAMLKTVIKYLDQSKTAAEKVQDPPVQTQXIGSSIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R168</accession>
    <name>A0A1E5R168_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zinc finger transcription factor YRM1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4049</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R168">
        <property type="interactions" value="1374"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R168"/>
    <dbReference type="InParanoid" id="A0A1E5R168"/>
    <dbReference type="OrthoDB" id="3972159at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052693">
        <property type="entry name" value="Yeast_MDR_Regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31405">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31405:SF8">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="183"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="999"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1002"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1019"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1044"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1064"/>
            <end position="1075"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1167" mass="132565" checksum="5CD9F2819C1B6E99" modified="2017-01-18" version="1">MNGNNSYEGNANMKRVASFSQDQQSVQGTLYQSPSWRPWVSDSPEVPKRSNLNNNLFTQHDTQLDSTASLTTLAXKKGRLESIPSATDTNETRPNLSPQQLFPNSHVPNTMLINVSMQNNQMNLXAANLFDSSLSSXPSQPMHSPLSKGTSAKLNSHAHDSTPPNTNGNGKAPKKTRNKLIKSCLYCRRRKLKCNKQHPMCGTCLSRNFKECIYLTPPVDXASKANKSDTTTHSNQAKQKNKKRKISNATESILESNDGGGLNTADNDRSKLLTNQVSQNPAIGTAPFGSETINMQVPGEVHDSYSASTRDFSDVATERTLSPLQSQASHTSERIYQENALKKSNLLSIHGKTMPNVRHNKILDLGCLTLKNERYMYFGATSVKSSVTNSDSSFLTKLQEGWKEYRKYRMKGKRLTKYSLMREIATLERTDKVPTMERMLQDLPPKIDIIDLYVDMFFKSSLHKFFHILSEQKVKEDVRKVFKTDSLTGKIVKINFTGKKNFYTVGVILMILAFTFYDITMPSSLLEFFVFLEGITTGKPMFVERAQFLCLKTLHRIITGCTGGDGSHLLNLVGSLCTTVIGLGFHRDVYEIYQRDVDYLGGDVQILKNIFYWTLFADLFVSTQYGKQLFITPDLYVPENLLLPEKYEEQSTESTDFSLAKHQQHDSFDDSINTQRKRLDVLKLFLYHMRCIVAQVYQPLDTPDVLQLIRTMSKGFDDIFMQLEKYFVIENNETDLFDYLLLPVYFQLQLALSSILMTLSNKNTTYAANNIIKFALSTMKVCTACASKSAKQQKLFKSKNKDTYQKLKKLCVGKYIEPDILLAPYVRYTTLRRAILEYYKVLYKATAVITEDTFSLREILQAFIVENSTKEGPYKSASVLSSSNGKDTSFFHSEFSLNYLNEEAMDTTKFCGLQLYKWFCVHSDELFLIETKLFVSNAKFSPEIYYMKAVEKVTKMAFKNFVCDLQKLINGENNEGDKQGTRTSTEVFSQIRYPGKGLARRKSLEADKYSQKKNDNLLRANNSQNNSEPKTAANERSTTGGKNGHLEHNPGHKDMPESQLDHFSVSTPSTTSHVTQDQPESQSIAGHRRYQQIVDYTESDANSESNQFKEIPFLPGPLDYFFNPENSSLNTENVFREIDVLFESFDFQEDSDLSMLLDVQKSSKQED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1C2</accession>
    <name>A0A1E5R1C2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g4258</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1C2"/>
    <dbReference type="InParanoid" id="A0A1E5R1C2"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000062">
        <property type="entry name" value="RB-associated KRAB zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF19">
        <property type="entry name" value="YY2 TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="403"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="431"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="99"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="200"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="301"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="496"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="698"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="101"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="320"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="361"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="502"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="706"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="720"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ80704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="738" mass="81896" checksum="4DF47F78C585BD0B" modified="2017-01-18" version="1">MLKNNIDTNHNDSGNEGIELIEKSDGVEKLEGVTKFEGIEKMAKPETIGVHNDSLLNLGSDNIKQEQFNPLTSSAAPIHQPTQFYSQTQVNLDPIMKRDNTSSSTETTTNNITTTANSAGQNSFVQPGSLMPYGSSNFAQNTNYLSSLNAQQHQAYQTVLPNSRLVHNNEYIHYYDKQQQQQQQQQQSQQQQQQQQQQQSQQQQQLQXPGPVLSQNTSSHPVTYLPYTNPAQGGIGGVSSIDDFYRRNTHTAVSSHLKSWPSVSHIDDLRAATYSANGGQLEYGMYQATYPPSYPTTTATATSTSFSTGMXPPPGVPNFTPYSDQQNQYYPNHPKSYSLTYEEQDSSINKSNENSNKEVMSRNSSDNSIETEAKIEEVSTLKTAKNSKSLRDSNKSVRTLTSYLCDVCQKRFKRPSTLATHQNIHTGKRPFACNFPHCHKTFNVKSNLARHMKCHSRKLRNENSITAIYNNRYYIPSNPSSLTQHQYDSQILQRADVRRAQDQQQQQQQQQQQQQQHGFPYSTQNFNKTYPEAFMASSTGNSHLPQQTQQRQYPPLQPLLSVTTQLPVSNSALLSGNMTSATGTSRLGSPLLLSRQSSFTQNGLPGIQDQTPALLTSAYGTAAPTAVPLSNQLHTSHLAYPSVTHNKSLLGFGSPYMYQSSSHSATATANVSAITANNTNTNFGTLFPANTAPPNIAKSQTGIEPNTNNTTKYTSTKNNGPTTWRDGSNITQERKSFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1J7</accession>
    <name>A0A1E5R1J7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 11">Triosephosphate isomerase</fullName>
            <ecNumber evidence="6 11">5.3.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g4249</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="6 11"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1J7"/>
    <dbReference type="FunCoup" id="A0A1E5R1J7">
        <property type="interactions" value="697"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1J7"/>
    <dbReference type="InParanoid" id="A0A1E5R1J7"/>
    <dbReference type="OrthoDB" id="6715177at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00189"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046166">
        <property type="term" value="P:glyceraldehyde-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000025">
        <property type="entry name" value="Triosephosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_B">
        <property type="entry name" value="TIM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022896">
        <property type="entry name" value="TrioseP_Isoase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020861">
        <property type="entry name" value="Triosephosphate_isomerase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139:SF41">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="Triosephosphate isomerase (TIM)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00171">
        <property type="entry name" value="TIM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="9 11" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 11 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ80747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="252" mass="27447" checksum="FA130EB539FD3D4A" modified="2017-01-18" version="1">MARTFFVGGNFKLNGSKKLIHEIVERLNESKLDSNAEVVICPPATFIDYTASINKKSPQVQVGGQNTYTKASGAYTGENSVTMLKEVGADWVILGHSERRAYFKETSEEIAEKTKFAIDNGLKVILCIGELLEERKAGITLDVCKKQLEPVLAKLSAQDWDKVVIAYEPVWAIGTGLAATPADAQDTHKEIRDYLKEKLGESKAESIRILYGGSANGANASTFKDKVDIDGFLVGGASLKPEFVDIINSRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1N3</accession>
    <name>A0A1E5R1N3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 15">NADPH--cytochrome P450 reductase</fullName>
            <shortName evidence="14">CPR</shortName>
            <shortName evidence="14">P450R</shortName>
            <ecNumber evidence="14 15">1.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AWRI3579_g3952</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Involved in ergosterol biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>2 oxidized [cytochrome P450] + NADPH = 2 reduced [cytochrome P450] + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24040"/>
            <dbReference type="Rhea" id="RHEA-COMP:14627"/>
            <dbReference type="Rhea" id="RHEA-COMP:14628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:55376"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="EC" id="1.6.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="14">Binds 1 FAD per monomer.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="14">Binds 1 FMN per monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Endoplasmic reticulum membrane</location>
            <topology evidence="14">Single-pass membrane protein</topology>
            <orientation evidence="14">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Mitochondrion outer membrane</location>
            <topology evidence="14">Single-pass membrane protein</topology>
            <orientation evidence="14">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Cell membrane</location>
            <topology evidence="14">Single-pass membrane protein</topology>
            <orientation evidence="14">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the NADPH--cytochrome P450 reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14 15">In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">In the N-terminal section; belongs to the flavodoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.6.2.4" evidence="14 15"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1N3">
        <property type="interactions" value="819"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1N3"/>
    <dbReference type="InParanoid" id="A0A1E5R1N3"/>
    <dbReference type="OrthoDB" id="1856718at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003958">
        <property type="term" value="F:NADPH-hemoprotein reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="NADPH--cytochrome P450 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NADPH--cytochrome P450 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03212">
        <property type="entry name" value="NCPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
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    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
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    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
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    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
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        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
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    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
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        <property type="entry name" value="P450R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
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        <property type="entry name" value="NADPH--CYTOCHROME P450 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000208">
        <property type="entry name" value="P450R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 14 15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 14" id="KW-0274">FAD</keyword>
    <keyword evidence="1 14" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2 14" id="KW-0288">FMN</keyword>
    <keyword evidence="14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 14 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="6 14 15" id="KW-0521">NADP</keyword>
    <keyword evidence="9 14 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="13 14" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="10 14" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="12 14" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="17">
        <location>
            <begin position="63"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="18">
        <location>
            <begin position="279"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="244"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="258"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="69"/>
            <end position="74"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="125"/>
            <end position="128"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="199"/>
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        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
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    <feature type="binding site" evidence="14">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
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    <feature type="binding site" evidence="14">
        <location>
            <begin position="456"/>
            <end position="459"/>
        </location>
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            <name>FAD</name>
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        </ligand>
    </feature>
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        <location>
            <begin position="492"/>
            <end position="495"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="557"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
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        <location>
            <begin position="622"/>
            <end position="623"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
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        <location>
            <begin position="629"/>
            <end position="633"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="703"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR000208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OEJ80792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="703" mass="78787" checksum="110EB9FD66DBC63D" modified="2017-01-18" version="1">MPLGLDTLDFTVLVALIVALTLYLKKDQILEFLQGESDDPAFSVSNSDTRDLKEVVQQXNKNFVVLYGSQTGTAEDYAKKFSKELNAKFRNQINVMCGDIENFDFENLHELSSETDVKIVAFFISTYGEGDFPDSSVHFEDYLNTLEADDAVLKGTKYLMFGLGNSTYEFYNGAAEKTVEKLSKIGGATMLGKFGKADDGMGTTDDDYVNWKENVLEILKDELKLDEKEEVFKSSFEYVKLDSTQENSISHGEPNKQYLPTAQLNTNNEGKQLGPFDAQHPYVAPXVFSKELCSPASGRNCIHAEFDLTGSNMKYSTGDHLGVWPSNSDEHVAKFLKVFKLNAAEAFDLKSLDSTVKPPFLTPCTIGAAVRNYLEITGPISRQLFASVAQFVPQDTHSXLKQKIAELSKNSELYHKSITSKYYNFSDALVALDSTLDWSIVPWTFLLENIPRLQPRYYSISSSASMEKQTVHITAMVESMINAENPQQAITGVTTNLLQNIEIDQNNDTVXKKFVHYDLKGPRGLYNNFKLPVHVRKSQFRLPSNPNTPIIMIGPGTGVAPFRGFVREKVQMTLKPNSENTKFGKALLFYGCRNDDDFLYADEWTEYAKKLGDTFEMFVALSRSSKTKKTYVQDKLFENADQVLDLLGKGAFIYICGDAKNMAKGVHDTLCNILATGKSISNEDASEMLKMMKVAGKYQEDVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1Q3</accession>
    <name>A0A1E5R1Q3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">choline-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="9">2.7.7.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">CTP:phosphocholine cytidylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Phosphorylcholine transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g3875</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.15" evidence="9"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1Q3"/>
    <dbReference type="InParanoid" id="A0A1E5R1Q3"/>
    <dbReference type="OrthoDB" id="3972858at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004105">
        <property type="term" value="F:choline-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02174">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000147">
        <property type="entry name" value="Cholinephosphate cytidylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041723">
        <property type="entry name" value="CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045049">
        <property type="entry name" value="Pcy1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739:SF13">
        <property type="entry name" value="CHOLINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739">
        <property type="entry name" value="CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="13">
        <location>
            <begin position="148"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="328"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="33"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="102"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="368"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="14">
        <location>
            <position position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00076205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ80812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="412" mass="47184" checksum="6299174A9A931D59" modified="2017-01-18" version="1">MANFKSNSFSNLFKLGRKKRSHDESSAGDATESESQNPLHSISSVVEMNSLKSNSSNKNKNKRRKRMQSNSNFDETEENSXXHSEAEDPHSEESSSDVETVEEQKHAEYLEHEKEYDALLPEKYRKYRPSGFKFNLPHEDAEKPIRIYADGIFDLFHIGHMKQLEQCKKAFKNVELVVGVPSDKVTHKLKGLTVLSDKQRCDSLRHCRWVDEVIPDCPWVVTPAFLDEHNIDYVAHDDIPYGSGDSDDIYKPIKEAGKFLVTQRTDGISTSDIITTIIRDYDNYLIRNFARGATRQELNVSWFKQNELTVKQLVTKLLKKRRGFRRGSVGKKDAVKGKQSTAGKLEEHXKEEGEEGEEYSSSNSNSSNDEEEEEYEEAEQTPEPESSTSPTGEKRPSDEFKEFARKFTGEHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1R9</accession>
    <name>A0A1E5R1R9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Protein phosphatase methylesterase 1</fullName>
            <shortName evidence="6">PME-1</shortName>
            <ecNumber evidence="6">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3987</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Demethylates proteins that have been reversibly carboxymethylated.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>[phosphatase 2A protein]-C-terminal L-leucine methyl ester + H2O = [phosphatase 2A protein]-C-terminal L-leucine + methanol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48548"/>
            <dbReference type="Rhea" id="RHEA-COMP:12134"/>
            <dbReference type="Rhea" id="RHEA-COMP:12135"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:90516"/>
            <dbReference type="ChEBI" id="CHEBI:90517"/>
            <dbReference type="EC" id="3.1.1.89"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1R9">
        <property type="interactions" value="836"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1R9"/>
    <dbReference type="InParanoid" id="A0A1E5R1R9"/>
    <dbReference type="OrthoDB" id="194865at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051723">
        <property type="term" value="F:protein methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016812">
        <property type="entry name" value="PPase_methylesterase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14189:SF0">
        <property type="entry name" value="PROTEIN PHOSPHATASE METHYLESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14189">
        <property type="entry name" value="PROTEIN PHOSPHATASE METHYLESTERASE-1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF022950">
        <property type="entry name" value="PPase_methylesterase_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0719">Serine esterase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="8">
        <location>
            <begin position="106"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR022950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR022950-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="394" mass="44917" checksum="A97CDDC435F5E61F" modified="2017-01-18" version="1">MDSLHKNLLLKKFKMAEAGLNDKASTGETQNEELDEVXELPSLPGKSTKRITEQEIVLEKTVRSLPQWSDFFLHSMQLQKNDHKFQIYFQLPDKIDDPATKTYPILIFHHGAGSSGLTFAQLCTHLNQLYKGKVGYLVYDARGHGQTEYTGTDPSKQDFSLIEFIQDEYVILDDFYHNKVIPHFRNDQKLLTKIKLLLVGHSLGGSIATFLFDHISKSDKSKELRKKIMGVVMLDIVEEAAIAALNTMDRFLAQTPHSFEELSDAIDWHVVRGMPHKRNSAEISIPPLFNEAIERVTNLKVFEKYWGSWFTGLSSKFVEIATSKLLILAGNENLDKELIVGQMQGKYQLVVFQQSGHFIQEDCPKETAITLQDFIQRNDNRSTVIKSNWGSVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1X1</accession>
    <name>A0A1E5R1X1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative lipase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4172</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the putative lipase ROG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1X1"/>
    <dbReference type="FunCoup" id="A0A1E5R1X1">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1X1"/>
    <dbReference type="InParanoid" id="A0A1E5R1X1"/>
    <dbReference type="OrthoDB" id="5368485at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016445">
        <property type="entry name" value="Rog1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF20">
        <property type="entry name" value="LIPASE YDR444W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005412">
        <property type="entry name" value="UCP005412_abhydr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF676" evidence="4">
        <location>
            <begin position="161"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="389"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="485"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="389"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="399"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="428"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="493"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ80908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="633" mass="72462" checksum="F149813267C3099B" modified="2017-01-18" version="1">MKSACSLYRDEDVVNCGEVFRYHIRVSNCTENTEVIFRFKNEESILLRPVYITGPFSLYVDCRPYQYDEDKEFPELIQFDADVRPEQHFQGLLKCNKNSWNGDCYEWTIDVISQVAINPNCTLRYSLELLDSSNSKTQFLPINVSRESTMDLWECLPLRPKNEVHLVLITHGIFSNIGADMLYLRDKLLKRGLEKNLNLLVRGCMDNVGKSHKGIEYLGTRVANYFIDLVEKEAKKYNITKVSFIGHSLGGPVQSFAIAHVYEQIPEIFEKIQPINFIIMAAPMLGVAAEMPAYASMALDLGGLGKTGRDLTFKHRRFFGRPNTQSNKPVIETTAKSPVFQKFQNRTLYANVLNDGIVPLRTSALLYLDWHTIDKLLVIFAQNGLNKNKETKHADNHTSSEIPELNPKSTKSFATSSNFEDSSESEMNDEHKSEEIAKDQENDELKNNESASERSNNITPINLADLIKQKKLSKFSRIQIINDSEDSENEVSDDSKQAEGEIQKQSESFNPPPKASALLSAAQFMFAPLPTEKYIISPSSRTADIILHDKVYSPSQLPAPHYVHRKTIKKLIYPKDKVHRKEERVARYWQEKMDWRKVLVVLQPDSHNSIVVRRRFVNLYGNVVVKNLVETHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1Z9</accession>
    <name>A0A1E5R1Z9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1Z9">
        <property type="interactions" value="163"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R1Z9"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018479">
        <property type="entry name" value="Lrs4/Mde4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10422">
        <property type="entry name" value="LRS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="84"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="261" mass="30246" checksum="805CD0C7FB5EC8AC" modified="2017-01-18" version="1">MIASLAENTMSDEKQVSHPSANFNSLAHLVAEYYSLVIESERIYHRHLRNVITRKKDKETKSKTAKLMEEVKCEKQVDGLFTQIMDLQKKNEHLQQSNKSDREKYESKFDRQNKKIQELYKLVAEKSSQPXQVVPQDKQSSEKAIHLLTPRKPMGFWSSYDKRXNQRSXNENIGTTAVSIESEVISDKRKFREVLSQNTIFDKSLEDIVIFNGKRKPTVQLNNGNPSQNIDKIDEVSKENISNASEKSTFSNETFKKLRQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R204</accession>
    <name>A0A1E5R204_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Non-structural maintenance of chromosome element 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g4174</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R204">
        <property type="interactions" value="43"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R204"/>
    <dbReference type="InParanoid" id="A0A1E5R204"/>
    <dbReference type="OrthoDB" id="4050598at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014803">
        <property type="entry name" value="DNA_repair_Nse5/Nse6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08691">
        <property type="entry name" value="Nse5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ80907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="607" mass="71364" checksum="11B476108EC14842" modified="2017-01-18" version="1">MAGYYAAPTEKSLEKPRYFVELTAQRFADFELMNSLYSLKNFDSLLFLLENPIDTQTTQGHQFSIKFVVPSFNILCVLYTLATLPFQNEDKRLEKLHQHSVSTETLQTRSIKLLNEYILILQHFDTDLYDEKDLISLRCQFFILIDYLFHIDTQSSHSQGAFSQEQKLDKFIQKYSSQSESMRNISLPSNCRRQDVKKQLAHLQYLNSVYTPRLVSPFHLNDSSSLKIADRLLFPLQFTTQRFWTTVEFIVAGSKLESDKPIQYLSSYWTPVLNSIFQILIMREHFYVTFELKGDVFSKDENFQNLFEYDSKGLLEQLRAGPVATAFHSLKKFHGWNSKFRELLFPXNPLQTVLLTEPFYLSEVTIDQGIAKRFLEQGSRSFAKKQKSASLPVLVSNSSHLSEKAQETFDFFDNLDENYSLLVKTILTCFISFEKHVAYYLAKIDSIKKYRSILKDSFVFSDDFFEQFAMFLIKECLMGPAEFQRYFSVDEIWENIETYEKNEFNLEYLNVVWFFSIAILFYISFDELDQRIYHIEELPKVQGEMYSFIVDLMEDAQNEQDNSKELTVTCLHNLITILKIIEKVYPNLIDXALYKEFLNEMSAFKFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R216</accession>
    <name>A0A1E5R216_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">dihydroxy-acid dehydratase</fullName>
            <ecNumber evidence="14">4.2.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AWRI3579_g4052</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:24809"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:24810"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-oxopentanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:27694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:27695"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IlvD/Edd family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.9" evidence="14"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R216"/>
    <dbReference type="FunCoup" id="A0A1E5R216">
        <property type="interactions" value="466"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R216"/>
    <dbReference type="InParanoid" id="A0A1E5R216"/>
    <dbReference type="OrthoDB" id="3851628at2759"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00057"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00061"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004160">
        <property type="term" value="F:dihydroxy-acid dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.80:FF:000001">
        <property type="entry name" value="Dihydroxy-acid dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.80">
        <property type="entry name" value="IlvD/EDD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00012">
        <property type="entry name" value="IlvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050165">
        <property type="entry name" value="DHAD_IlvD/Edd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042096">
        <property type="entry name" value="Dihydro-acid_dehy_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004404">
        <property type="entry name" value="DihydroxyA_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020558">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056740">
        <property type="entry name" value="ILV_EDD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000581">
        <property type="entry name" value="ILV_EDD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037237">
        <property type="entry name" value="IlvD/EDD_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00110">
        <property type="entry name" value="ilvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002068">
        <property type="entry name" value="PRK00911.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21000">
        <property type="entry name" value="DIHYDROXY-ACID DEHYDRATASE DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21000:SF5">
        <property type="entry name" value="DIHYDROXY-ACID DEHYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24877">
        <property type="entry name" value="ILV_EDD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00920">
        <property type="entry name" value="ILVD_EDD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143975">
        <property type="entry name" value="IlvD/EDD N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00886">
        <property type="entry name" value="ILVD_EDD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00887">
        <property type="entry name" value="ILVD_EDD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydroxy-acid/6-phosphogluconate dehydratase N-terminal" evidence="17">
        <location>
            <begin position="56"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Dihydroxy-acid/6-phosphogluconate dehydratase C-terminal" evidence="18">
        <location>
            <begin position="392"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00052865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF24877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OEJ80940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="586" mass="62811" checksum="158FF0D9451F4FFA" modified="2017-01-18" version="1">MSALYTKRLFSTNNTKILNEAKRLNKYSFVVTEPVSQGASQAMLYATGFNKADFSKAQVGVGSCWWSGNPCNMHLLDFNNRISKSVDAAGLKAMQFNTIGVSDGISMGTFGMKYSLQSREIIADSFETIMMAQHYDANIAIPSCDKNMPGVMMAFARHNRPSIMVYGGTILPGHGTCGSSKIPEKLDVVSAFQSYGQLISKQINQEERNDIVAHSCPGAGSCGGMYTANSMASAAEVIGLTLPNSSSYPAVSKEKLAECDAMGPAIKKLLEMDIKPKDIMTKEAFENAITYIVATGGSTNAVLHLIAMAHSAGVSITPDDFQRISDKTPLLGDFKPSGKYVMADLQQYGGTQAVVKFLYDAGFMHGDTLTVTGETLKERANNTKPMPKDQPIMKPLSAPIKSSGHLQILYGSLAPGGSVGKITGKEGTYFKGKARVFDEEPSFIAALERGEIKKGEKTVVIIRYEGPKGGPGMPEMLKPSSALMGYGLGQDVALLTDGRFSGGSHGFLIGHIVPEAAEGGPIGLVHDGDEIIIDAENNKIDLLVSDSEMQERKSKWTPPAPRYTRGTLSKYSKLVSDASHGCVLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2B5</accession>
    <name>A0A1E5R2B5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Nonsense-mediated mRNA decay protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4113</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2B5"/>
    <dbReference type="FunCoup" id="A0A1E5R2B5">
        <property type="interactions" value="1029"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2B5"/>
    <dbReference type="InParanoid" id="A0A1E5R2B5"/>
    <dbReference type="OrthoDB" id="27832at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039762">
        <property type="entry name" value="Nmd2/UPF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007193">
        <property type="entry name" value="Upf2/Nmd2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 2 UP-FRAMESHIFT SUPPRESSOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839:SF7">
        <property type="entry name" value="REGULATOR OF NONSENSE TRANSCRIPTS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04050">
        <property type="entry name" value="Upf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIF4G" evidence="4">
        <location>
            <begin position="32"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="MIF4G" evidence="4">
        <location>
            <begin position="379"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="MIF4G" evidence="4">
        <location>
            <begin position="588"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="821"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="829"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="851"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="874"/>
            <end position="910"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ81045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1071" mass="123324" checksum="CA0350A88BBCE48A" modified="2017-01-18" version="1">MMSRAELYELNSKAWSFPPTFPGLASKLDTSIKKNTGFIKKLRLGITKDNSPGFLEDLKNVSLSKYLSEVCSTLASDTLLKISNKQEDLYAAVQIISALHQRFGAEFTEPFFALWLNNFDCEVRTKDAATDSEIVSSLKSVASQIFILCELQVVQCVPQIDDLLPFKNSLPKFLLGKLNKKEHLLISVLKELVNYNFKSGISTYIISTRFVKNFPEVFESAESHAPFHGLNEDLLQTLKAMFKLLAQTSLDQVNTFYAKLPKLLKEVEKSQIRTGKATNEYTEKYDLAKTVLNYFYQAAEILCPIYAISVPAPLDEAKMEEELIEDSEVSELITNKIKPLHKRIWETDDVQKFYEVLMPLTLPKEGEKRFYQEDEHIRREARNAFFQKLEEVEDVKVVDSLATEYNVIGIDGTSTRKRLLNFVIELQDWNKINIYARFLATCAPIFPDVIEKFVEYLDNGFRSQLHSNKINVKNILFYCELVKFRLVPSFMMFHKLRTLIINLNVPNNIEIINILFEQIGRLLLNHPDYQENMNKMIELLKEKSKSFNLSMPAKLQIESTLLLLFPPSLKNINGFDNSNNRQENPEVNFLYYLMRKEIHSLSTRVISELIKRANWNDEKVFGALLDIFTQPECIDYNSIERLTKILSTLQGRYKFFVNQVIDTVLENIQCGLENNNFAKNMTRLSQAKYLVELFNHDSIKFDDLLDTAYKFVKFGHKSLFLDDKSDLDLPNNYFRINIICTLLLSLKFESKHAKRSIQQKLALYLRYFEYYTFTKERPFPMELQGKIAETFKYVSAHLEEDYQRAESLKECANKLIESLKQMDQHKATEEDEDEEEEDDDEDDQSDFEDELSVRGESRGDVTGDELTSDSSFSDSDDSEDSEDDSDTSSESGESDSETESESDSVSEEEALAIARELELQKLSEEYNQKMQTEEEVAATDDFEKQFQAMMQESMDSRRNEKFMSNSAIPVNLSKASSVINYGSQPELSSTISQGKGPGESDLLQQSSFLVNPSTPGKVAFKFLSKNGKKINTKTLELSENVSFVSNVIEEDNKRKDERERIKEYVLKNLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2C7</accession>
    <name>A0A1E5R2C7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ATP synthase F(0) complex subunit e, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4115</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Subunit e, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. Part of the complex F(0) domain.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ATPase e subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R2C7"/>
    <dbReference type="FunCoup" id="A0A1E5R2C7">
        <property type="interactions" value="114"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2C7"/>
    <dbReference type="InParanoid" id="A0A1E5R2C7"/>
    <dbReference type="OrthoDB" id="2125027at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008386">
        <property type="entry name" value="ATP_synth_F0_esu_mt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05680">
        <property type="entry name" value="ATP-synt_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4 11" id="KW-0138">CF(0)</keyword>
    <keyword evidence="5 11" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ81049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="99" mass="10571" checksum="F44F0C60AEFC6A52" modified="2017-01-18" version="1">MSQTVNVFRYSALALGLLVGLKTDMRASSDAHTAAELHKQEELIKQAKEAYAKAHAPAPSAADSAKQATTAAPKFDLEDEKADFAQLILSSVEALEKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2G1</accession>
    <name>A0A1E5R2G1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">37S ribosomal protein S22, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3900</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Mitochondrial ribosome (mitoribosome) assembly factor. Binds at the interface of the head and body domains of the mitochondrial small ribosomal subunit (mt-SSU), occluding the mRNA channel and preventing compaction of the head domain towards the body. Probable inactive methyltransferase: retains the characteristic folding and ability to bind S-adenosyl-L-methionine, but it probably lost its methyltransferase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2G1">
        <property type="interactions" value="318"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2G1"/>
    <dbReference type="InParanoid" id="A0A1E5R2G1"/>
    <dbReference type="OrthoDB" id="421327at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052571">
        <property type="entry name" value="Mt_RNA_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015324">
        <property type="entry name" value="Ribosomal_Rsm22-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016522">
        <property type="entry name" value="RSM22_mit_bud"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13184">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13184:SF5">
        <property type="entry name" value="METHYLTRANSFERASE-LIKE PROTEIN 17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09243">
        <property type="entry name" value="Rsm22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007797">
        <property type="entry name" value="RSM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="360"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="451"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="373"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ81097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="694" mass="78451" checksum="3F0D66E8E379BC64" modified="2017-01-18" version="1">MLRTRCNAALFRXKNVSSTTFSQFVRLNSDSSAFKISDDIPQKMAGVVVDSRTDNSRVITREQAYQKYKEPDWTKGIGTERKSKFDPRTLAGKMSLSGTLRLNPTVAEAINDHILKPHVPNHLRQSAANYYLELQESKIHRPCKTPMEVDSHIASIFLQNYGSVYQSLYELRKRCIDAGQVFNPQRVLDVGYGPATGIVALNDLMGPEYXPSLKDSVVVGSVEMQKRAKIILSRQYNEVVEPEMXGNESVATAXKGEKGTDDIEETDRLYGNIDTKKIRINTNLKKRTPGKPKQYDLIILQHQLLKNEHEFPLQVETNLQHYLQLLAPGGHIVFVERGNPMGFEIVARARELVLRPEKHEDEPLVGKPPRLWSTESAENTPLTGNASXSNXVYLKVVAPCAHHRTCPLQVGKPLYYEFKKFGNLNFCNNQKKIQRPKFSIELKRGKILGSTWSESPNEPGVATNPEDQGISGRRGATDYEILNYTYLILQRVKNSEVVEQSNLTEKESSNEFSGVAESISREIDTGARVVDVPLKKKGHVVLTLCGSSGDIEKVTIPKSFNKEIYYDARKSHKNDIWVHGGKTTVKSHNLLKTQRFEKYLKMEKALYKKSLEQVKKNEFKLHEQLDDLRSGVGESGIGANNRGAQTQLDQQIEILAKLHGNNFNLSEKGKKILKHKTKNKVDLRKIYEQTDDSX</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2G7</accession>
    <name>A0A1E5R2G7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g4220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R2G7"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="485"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="2">
        <location>
            <position position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ81080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="518" mass="59593" checksum="52C45D6536E04063" modified="2017-01-18" version="1">MSLXVAPXSQEEYAHPSMNMHCPDDVCKNIENSEAVFSQKXGYKRQKGRKRAKLRQLFHKHDKEQNPNTANSDFDSPKPXNQRSLNYNDEDVENPSLNGGNRCIGRRRDHIRKTIWDFFGRETYPSDNNYTPRIDRQNVLEQFTKQESKATAELCNFAPSQDVLATNYMSFSSHKDISEPKVSESGNTGSESSPPLFSAQYTSNFSKNSSIDNSPFSHEHASGVSQNTSMNSRDVEKPVPEKTDNKCSTRELAFCSSKITNKDKECIKKINNFLAEQKLPFVFDQNMEFVKDHLATXENVNRKKVAFVAKEFSTPTSTLNFEQREFLXAPKKSTPXKTVSALQADMTVKFAKNKKFSLENDLFFKDCISQXNLSNLNDTXLXFEHXENQTFKRFTKMKSHTRFKEECLKDFDIEDLEEGHDDLNKTFEARNLKKKSKFYEGKALVSNPELLKNCEDKGNMTCKGLKKAGKYMDCAELFNIDKVSQSNQEEIEXEKKPDNQEKCTINMQRLKMEDIEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2J3</accession>
    <name>A0A1E5R2J3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative ATP-dependent kinase TDA10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3851</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2J3">
        <property type="interactions" value="339"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2J3"/>
    <dbReference type="InParanoid" id="A0A1E5R2J3"/>
    <dbReference type="OrthoDB" id="347435at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ81125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="316" mass="35739" checksum="BABFF18BA21BB053" modified="2017-01-18" version="1">MSTTKHEINQADVSFAHPKTTLDVATSYVEKCYSQYQTSKRNPAGSESQPPLIVFVSAPQGAGKTYLTTKLREKLNTRGYKCLAVSIDDFYLTHADQAKINXTVGVDNPLVNGRGLPLTHDLPLLSNFIHQLFYENKASEGKITKHFVQVPRYDKSKFNGEGDRLAQFDKVATPLDIVLLEGWFLGFDPLPDESKIQNKNMIIVNKELEKYASVLWNNPLIQKYNNTCGIIVKCSHSISIDSLVTKWRLQQEHAMIEKTGNGMTDEQVKNFVARYLPSYDLYYNNLVEMGKRGDLIVEINPDRQLVGFKARCGETE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R2J7</accession>
    <name>A0A1E5R2J7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative acyltransferase CST26</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g4212</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ81104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R2J7">
        <property type="interactions" value="276"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R2J7"/>
    <dbReference type="InParanoid" id="A0A1E5R2J7"/>
    <dbReference type="OrthoDB" id="189226at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036149">
        <property type="term" value="P:phosphatidylinositol acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07990">
        <property type="entry name" value="LPLAT_LCLAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032098">
        <property type="entry name" value="Acyltransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983:SF16">
        <property type="entry name" value="LYSOCARDIOLIPIN ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16076">
        <property type="entry name" value="Acyltransf_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="367"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="5">
        <location>
            <begin position="102"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ81104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="393" mass="44841" checksum="19AC6EF0223A12E0" modified="2017-01-18" version="1">MVVYKLKKICVLTFSIWVFLLGAVSIVSMQAVVKLVFRSNPVYQQQCLNYTKKSFILLLLTIFQIAAPSRIRVSTENSSVAPDTVSVDPRTGRIVSKXSKSSVLIANHQIYTDWVFLWWLNYTAKLGGNVFIMLKKSLASIPILGSGMKNFKFIFLSRKWDLDRVCLHNSLSDINANALGCGRISGNHPQSVSQDGTXIWGDRMYENKNNVHWPYCLMIFPEGTNLSANTRQKSEAFAEKIGRKPFRHVLLPHVTGLREILTKLRVSCDVVYDVSIGYSGVAANEYGQDIYKIMDVFMKGKQPKLVDIHIRSFKLDTIPVKDEQAFTKWLFDVWEEKDEYMEHYYKHGNFNLTNDTTSAEAECTVSLWEYLLILAMPVXSVLIAFLLVLKKVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R560</accession>
    <name>A0A1E5R560_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">N-terminal acetyltransferase B complex catalytic subunit NAT3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3648</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R560">
        <property type="interactions" value="775"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R560"/>
    <dbReference type="InParanoid" id="A0A1E5R560"/>
    <dbReference type="OrthoDB" id="10264728at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031416">
        <property type="term" value="C:NatB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004596">
        <property type="term" value="F:protein-N-terminal amino-acid acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051646">
        <property type="entry name" value="NatB_acetyltransferase_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45910">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45910:SF1">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="2"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ82008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="216" mass="25008" checksum="14F169E969536063" modified="2017-01-18" version="1">MTSINPFEMTDLFNLQNVNLDYLTENFPVEFYLEYLILWPTLFFQNSETLNTIPPIACQSLSSLQHSATTHPKHPISGYIMGKTEGKNLEWHSHITAVTVSKLYRRISLASQYLCVPFQMLSDSLNKVEFVDLFVKCNNQLAVNMYEKMGYSVFRRVVGYYNNSNDXNTASANLKNLKNNDEKDAFDMRLSMPRNDNKSIRKDGKNHRCLPQDVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5H8</accession>
    <name>A0A1E5R5H8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peroxisomal membrane protein PMP22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3744</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ82142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R5H8"/>
    <dbReference type="STRING" id="56408.A0A1E5R5H8"/>
    <dbReference type="InParanoid" id="A0A1E5R5H8"/>
    <dbReference type="OrthoDB" id="860at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="227" mass="25256" checksum="80BCF09EBF941AC0" modified="2017-01-18" version="1">MVQKSSPIESYNRLLTKNPLLVKVLTGSVLSGLNEIISSLLTGKIQLSVLSQKTLLMLIYGGCINTPINHFGYKYLIQVVSKLSKFCKLKSKKAINLMQLLGSLFIISPIQVCFLILTLSIVNSPIINSGGSIIALIKNARNNFKQFSSNLKDSVNDKFVKFYTSSFISSTVFVNVAQNFIEPEKWAIFFSCAYTSLNTAQNIYLKLKQAPPLIDSQEDGNDKDKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R609</accession>
    <name>A0A1E5R609_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pre-mRNA-splicing factor 38</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3746</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for pre-mRNA splicing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the PRP38 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R609"/>
    <dbReference type="FunCoup" id="A0A1E5R609">
        <property type="interactions" value="140"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R609"/>
    <dbReference type="InParanoid" id="A0A1E5R609"/>
    <dbReference type="OrthoDB" id="190958at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005037">
        <property type="entry name" value="PRP38"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03371">
        <property type="entry name" value="PRP38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0747">Spliceosome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ81973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="210" mass="25207" checksum="2D81DFED239A492E" modified="2017-01-18" version="1">MQQDFQVDTTLSTKQLNHQSTSVVIPHILRTRIHQSMFYKTNLSLGNMRGHTMLEFMPVLFKYFIDPKKQLKYCGGGEFAAFLLKLIEIKPTWEQVTSFIQVYCLKNMEIMKYAYALMMCYLRIQYYFIFEHDEQYPILQQWFRDGYTRYNKIKCLRFDVDTSNKNDFVTYHVDELIDRLMRASDIWGIPLGQCRWLVNEFDDTSNTEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7N5</accession>
    <name>A0A1E5R7N5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Altered inheritance of mitochondria protein 44</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3426</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7N5">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7N5"/>
    <dbReference type="InParanoid" id="A0A1E5R7N5"/>
    <dbReference type="OrthoDB" id="4065285at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="362"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="463"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="512"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="615"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="738"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="900"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="475"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="512"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="538"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="625"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="900"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="2">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="950" mass="105576" checksum="5FF0648CC783D65B" modified="2017-01-18" version="1">MSSQIVMRHPTTTRAKAFKGAQAEFIFPDDSPTSSDSSDNHHGTAFPNTREPHNESDSLFSEEDYYLSNHYLINAVASAKMLSKGSTKKNNYVMKRPSICPTSXSSDKGIXXQGVNKXGKSASMDSTPQCSPKPSSESSVPNSSNTGSSEYYSFANISDNTTSRSSARRAKTSSGSEVNSDSNDEHSYYNYSHNSTSPVRRISTRRSPGASARKRNSVKITRKATALGSRKALNPRISTLSFSGIXHVTLDPNAARKYQPDRDTPLTXVSGLGYGADMQASESPSATNGAFLINSDTSSENSSIILGSNKMKSANNYKFLNSPSAVSLSSVPTEKNNRIPELXTKLSSSSSLVMTPHALKKMNTLPHKIQPNGSVEDQDDYDDDDDDDDEDEMVETPNFSSIQQSNLNFMSCSTSSLSKKNSKLKRSNAVRCKGGLLNFFVRLGKFTKKSFRQWKQAIKKKFNKKNHDGKKLNRKTPTVSSIKKSPSMQFKTTNHLKRNFYTNVNIKRSMSTLQSASNSIKRXMSSNAPSPKKDAXSSLDTSVVQNTSNSQNPKRNSLRRSPSSIRRAVSIMNNLDKNTNSATLSPSKASLQLSNYIMPRSSSVVINSKAFRLDTCQEEEPQDETEGKASNDASKSDDVCAEHDDYVISTDRMTAFSSHSSSSSSASGDTVYEDAIDASPKAQIKSIESHPSQIQFFDSIYNSESEVDNDQVQDISQASTHEASSVYSAHRILSEIIEEEDEEEEEEEXEEEEEAIGYEEHDAEDLLKDIDHHEFILTSQPLNIIKEETEEXESKQIPKPQGSKFFSPXQFEIIDGEDSEDTQADKXWIDLDEEGDEEMQVEEEQILPMLLQHLFTQVLQKRIMMNVEISAMQRTYEFNETAQQLVRQVLSDYEGSTQFESNSDCDYESSNSEHELSSKRSTFSSNSFIEAARIGQSNVKRSLTMPISFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7N6</accession>
    <name>A0A1E5R7N6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein PCF11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3199</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7N6">
        <property type="interactions" value="518"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7N6"/>
    <dbReference type="InParanoid" id="A0A1E5R7N6"/>
    <dbReference type="OrthoDB" id="3972019at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005849">
        <property type="term" value="C:mRNA cleavage factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16982">
        <property type="entry name" value="CID_Pcf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000016">
        <property type="entry name" value="mRNA cleavage factor complex component Pcf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045154">
        <property type="entry name" value="PCF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054127">
        <property type="entry name" value="Pcf11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047415">
        <property type="entry name" value="Pcf11_CID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054128">
        <property type="entry name" value="Pfc11_Rna14/15-ID"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15921:SF3">
        <property type="entry name" value="PRE-MRNA CLEAVAGE COMPLEX 2 PROTEIN PCF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15921">
        <property type="entry name" value="PRE-MRNA CLEAVAGE COMPLEX II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21936">
        <property type="entry name" value="Pcf11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21940">
        <property type="entry name" value="Pfc11_Rna14-15-ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CID" evidence="2">
        <location>
            <begin position="2"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="568"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="278"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="568"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="696" mass="80004" checksum="DCDBD7A221C0EE12" modified="2017-01-18" version="1">MSQPDFLQEFAEQLDSLTFNSGPIISQLARSSEEHIEHAQQIVDLVETRIEKCLPKHKLYSFYVLDSICKNVGRPYTIFFQRNLPKLFKQTYLLVDNTTRQKLINVFKTWLNPNGNSGSLNXSPFDEDEYESAIFDKEVLKNLENFLIKASAVHEKNLKKTNTINYQPPTIPSLLKEIDRLIIICTDKVNLLQQRLSTAMTSLDPNSMPIQQQQQLQQVQTLITEEINKTQPKIMVLQELKGEIMKGNITMDALVQVQQQLRANFQQEHQEMMTLNTPSPQSAPALLQQQQQQQQQQQQPALLNQSPSQMGSIFGDEPAQPAHNLHEGALMTNIFGDIGSEQTSTGNDINNKNNTVQPIVDFMSSDSNSILKDLEQLKSSKMQKLFAKLKESKLIQEPDHDHSILTIWDKYCKHIPLDNRSRLNSSIRNLPDISLLQHIVKDLNSSKIDINYFINSPNFQMDSKQINENYKNCFPFIQLLYRYKPEKCSMCGKRFHTAPTPMNTMRTHNMASDSIHDHLDWHFRINKKINLNKGRAGLASRAWFLNDEQWTDLNPALETSHAAFNAETDNINSANPNGGXSXKRPYPSHAEEANDFANXNESNILSMNNIFDTEAKNESGLDYSEMANNIHKKHKNYKAMYIIIPDDLISQQVECQICKDTLQTTYDENIGQWVWANCLQDHERYCHATCYYESKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7T0</accession>
    <name>A0A1E5R7T0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3343</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R7T0"/>
    <dbReference type="OrthoDB" id="4064925at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="352"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ82954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="428" mass="49328" checksum="B35C83B57B6FC376" modified="2017-01-18" version="1">MVTKDITFGNSPYSIDPLSTTLTDLSDRSTNAHTAATAAAGMIQASSSVALPYMDYNSNEYPYSWNFCNNRFSRNEKNNDCMHYDSSFQHEDDELRYYFKPEESNLYLWHFVLYIKYTGEEICGMLYIKDSSEKNHSKEIYVDTTTTTTTTTTTTTSSSSSSSSSSSSSSSSSSSSSHYXXHNNNHISSGTXCKMVQTSDPTHVVEEYQVLIRLLTPNSNTAIPLNKPFDFTTYVDKDIFQKSRSLNTIFDHLWNFLYKNIVINKSTNNNITLHSGPLGKCWNRTILQDFKNLFPELYGLYHYTEMDQMYVQNWIRMQKYENELKRKPXMGNTTSEHEIINDIGXIPSTNDHNNTHSNAHKRNLGQQKTLGQQQKSLNNTDIKNNNGYHDTYHYQNNINTNYLNDKENDENTLYGNKKRKLNAQNYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R866</accession>
    <name>A0A1E5R866_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Glucose-6-phosphate isomerase</fullName>
            <ecNumber evidence="4 11">5.3.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3295</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11816"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="EC" id="5.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the GPI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.9" evidence="4 11"/>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R866"/>
    <dbReference type="FunCoup" id="A0A1E5R866">
        <property type="interactions" value="1160"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R866"/>
    <dbReference type="InParanoid" id="A0A1E5R866"/>
    <dbReference type="OrthoDB" id="5831190at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00181"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051156">
        <property type="term" value="P:glucose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05015">
        <property type="entry name" value="SIS_PGI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05016">
        <property type="entry name" value="SIS_PGI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1390.10:FF:000001">
        <property type="entry name" value="Glucose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000004">
        <property type="entry name" value="Glucose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00473">
        <property type="entry name" value="G6P_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001672">
        <property type="entry name" value="G6P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023096">
        <property type="entry name" value="G6P_Isomerase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018189">
        <property type="entry name" value="Phosphoglucose_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035476">
        <property type="entry name" value="SIS_PGI_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035482">
        <property type="entry name" value="SIS_PGI_2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001211">
        <property type="entry name" value="PRK00179.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469:SF1">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00342">
        <property type="entry name" value="PGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00662">
        <property type="entry name" value="G6PISOMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00765">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00174">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51463">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="7 11" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="8 11 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ83097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="557" mass="61574" checksum="C50AED7B783D1947" modified="2017-01-18" version="1">MASEQTFSDFKLATDLPAWKKLQSIYEKKGGKDFSVKDAFAKDSSRFANLSKTFQNYDNSKILFDFSKNLIDEEILSALIELAKEAKVTELRDAMFKGEHINFTEDRAVYHMALRNRSNKPMCVDGKDVAPEVDAVLQHMKEFSEQVRSGEWKGYTGKKITDVVNIGIGGSDLGPVMVTEALKHYSGPIKCHFVSNIDGTHIAETLKVVNPETTLFLIASKTFTTAETITNATSAKNWFLSKTGNDPKNIAKHFAALSTNEKEVGAFGIDTKNMFGFDSWVGGRYSVWSAIGLSVALYIGFENFDNFLKGAEAVDKHFTETPLENNIPLIGGLLSVWYNNFHGAETHLVAPFDQYLHRFPAYLQQLSMESNGKSVTRGNVFTNYSTGSILFGEPATNAQHSFFQLVHQGTKLIPSDFILAAQSHNPIENNLHQKMLASNFFAQAEALMVGKDAAQVEAEGATGGLVPHKIFSGNRPTTSILAQKITPATLGALIAYYEHVTFTEGAIWNINSFDQWGVELGKVLAKVIGKELDNKTTITAHDQSTNSLINQFKEWSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R896</accession>
    <name>A0A1E5R896_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">54S ribosomal protein L11, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3204</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R896"/>
    <dbReference type="FunCoup" id="A0A1E5R896">
        <property type="interactions" value="146"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R896"/>
    <dbReference type="InParanoid" id="A0A1E5R896"/>
    <dbReference type="OrthoDB" id="360689at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047865">
        <property type="entry name" value="Ribosomal_uL10_bac_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11560">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ83136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="260" mass="29027" checksum="0D42083EDCF38C4D" modified="2017-01-18" version="1">MLKSYFKTPFSLLKRYTTAATANVIGGSSANAAHVTPNVIASGSQTIIQDAASRITQKPITSKKTFLLDYYKHVMDTHPLVLFAHYNNLNKNENNMIRALCKDNSSTLTVLRNSIFKVYLRNSQRTDPASPVKAEEKSLLIKNHPLNQYLKGPTCCIAFKELNPAALNNLFTVLKKHQDKLFVIGAKLDNESLDLEKILKIKDLPSKNVLQQQLLGVLHMLSGAGLVNTLEMASSKNLYLTLKSHEQELEKEGKESESKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R8D8</accession>
    <name>A0A1E5R8D8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Trafficking protein particle complex II-specific subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3481</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R8D8"/>
    <dbReference type="FunCoup" id="A0A1E5R8D8">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R8D8"/>
    <dbReference type="InParanoid" id="A0A1E5R8D8"/>
    <dbReference type="OrthoDB" id="10256906at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990071">
        <property type="term" value="C:TRAPPII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034498">
        <property type="term" value="P:early endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056915">
        <property type="entry name" value="Ig_TR130_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056916">
        <property type="entry name" value="NTS_TR130"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045126">
        <property type="entry name" value="TRAPPC10/Trs130"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022233">
        <property type="entry name" value="TRAPPC10/Trs130_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056913">
        <property type="entry name" value="TRAPPC10/Trs130_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13251">
        <property type="entry name" value="EPILEPSY HOLOPROSENCEPHALY CANDIDATE 1/TMEM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13251:SF3">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24966">
        <property type="entry name" value="Ig_TR130_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24967">
        <property type="entry name" value="NTS_TR130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12584">
        <property type="entry name" value="TRAPPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23036">
        <property type="entry name" value="TRAPPC10_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="TRAPPC10/Trs130 N-terminal" evidence="5">
        <location>
            <begin position="8"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Trs130 NTS" evidence="7">
        <location>
            <begin position="299"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="Trs130 second Ig-like" evidence="6">
        <location>
            <begin position="664"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="domain" description="TRAPPC10/Trs130 C-terminal" evidence="4">
        <location>
            <begin position="956"/>
            <end position="1100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ82793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1100" mass="126979" checksum="5BBD3527A2FF61E6" modified="2017-01-18" version="1">MRYSCLNDQSIKVSYFDPISTFDSVQSEINQILPLKDLHWKPQGDTPVVTVKSLKVEFLPETSFADSKILNDVPFIKYIVVKCTSIDEYRSKIRPLIRQWVSTQVQENDEKDHNDPLVHIHKGILLLGSDIHDSKLFKTISLPEKISNDFPALPLYQLHTVYKSQTDRANHWNKYSSSLKKIILEVFEKRVSSLENANIQSPTFETHQQLLQLYLNFKIYGKANLHMRKIESLLTLESKPAGDLSLPIINSKDAGLAEWSSFTVFSALRYRLFSRLSVIECLHPNILADKIKELYRIIVIDFLYEIKSRFQSNELLHQVIYHACDEILDLRLLPALPLKHDESNHSLWKEMYGDILQIKRDSWMELADFHQSGITFCISPSAIPWKIDDVTKTTIESVDNAFQHFVRLTSEIIELFRETRGRTVDLLSMEIANIFYQTRSYEKTMKLLQSSHEYYRNAGWNLIASDVLVQYVTCLEKLEAKTVSIEEEDVPTISVLANSYLNLVTTPQGTDKITWWNKFLALNENAQLVYSLDLLMTTEVDEKVCLSASNTYAIILNILDMKVMTDVSIRSVKLLTKKKGKDQFVVFQKELFELATQSETKLELETTHIAYGEFELISLELVIGNTVFSKEYDEYKKILIHPLYSVNNFHVVLKEPCERNLEKNELELKTVNAERVENFECKIEILEPFSVDFAETNSKNFIVNSANFKSRIQYICKEEQSLELHVLQTLTFKRDGVLYSEVQIVKIDHQIEMSISVNDIYKSDAFYFNFTISAKYEPLHVVDTKLCCNVESKYEITEGYEAGDASSFLEVPEVENLQSFFQIKPIQKAFSYKDCFSLSVSYVSLSGMVNDYLTQKFFGRDSNFSHLKHIWESQILTQIEYHKSQFFNYNTIKISNYDRPQFEKQMQKIFPNESSIDYMLNLYETLFDGLEVEEDFNFSKRKAQALKKLTLSVTIPPPINNVVFSIEMTLDETYTGKTLIVGEPYSCTLKICKQFQAWGGLKLENNSSIYQCELDKGAGSEEWLVSGLKKFEIRNDMTTVNVTLIPIKRGYLRFPDISISPSESLNNTNANASTASLNHTAEVSKIETEFLNINDNIIVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R990</accession>
    <name>A0A1E5R990_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Trimethylguanosine synthase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Cap-specific guanine-N(2) methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3425</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78479"/>
            <dbReference type="Rhea" id="RHEA-COMP:19087"/>
            <dbReference type="Rhea" id="RHEA-COMP:19089"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:167623"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:78480"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78507"/>
            <dbReference type="Rhea" id="RHEA-COMP:19088"/>
            <dbReference type="Rhea" id="RHEA-COMP:19090"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:167623"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:78508"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78471"/>
            <dbReference type="Rhea" id="RHEA-COMP:19085"/>
            <dbReference type="Rhea" id="RHEA-COMP:19087"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:78472"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78475"/>
            <dbReference type="Rhea" id="RHEA-COMP:19086"/>
            <dbReference type="Rhea" id="RHEA-COMP:19088"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:78476"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. Trimethylguanosine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ83103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R990"/>
    <dbReference type="FunCoup" id="A0A1E5R990">
        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R990"/>
    <dbReference type="InParanoid" id="A0A1E5R990"/>
    <dbReference type="OrthoDB" id="3972642at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071164">
        <property type="term" value="F:RNA cap trimethylguanosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019012">
        <property type="entry name" value="RNA_cap_Gua-N2-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741">
        <property type="entry name" value="S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741:SF32">
        <property type="entry name" value="TRIMETHYLGUANOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09445">
        <property type="entry name" value="Methyltransf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="81"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ83103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="356" mass="40484" checksum="DBB7121B325A76FA" modified="2017-01-18" version="1">MLESNDLFKFLDRLILLPDFYKNSKEEFLAELIGEINAQVDSGKLLVDNTMQPERKDLTKYWVNRFKIFSKLEEEVIAANAKNEEESDVDSNLKQEQANDMEDDNDDDDDDQEDDDDMGQSFGSFAFHHSPKKGKALSPVVTHGVTKPSPHNNIYMTEELWYSVTPEDLAIFTAKWAYACLKDDLRIAMDVCCGGGGNTIQLAKHFPNCIGVDNNLQHLYCTFKNSETYDISERVSLLCLDWNDSNTLKKLARIYEGKINCIFSSPPWGGPSYLKQSTFDLEEGLFIVGGITKFLKSLFQFTENVVLFLPRNSNLKQLSEVTKKLLGNEAKCRVIFIKTDGHLKGVIVMWGKKLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA12</accession>
    <name>A0A1E5RA12_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2998</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RA12"/>
    <dbReference type="FunCoup" id="A0A1E5RA12">
        <property type="interactions" value="1197"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA12"/>
    <dbReference type="InParanoid" id="A0A1E5RA12"/>
    <dbReference type="OrthoDB" id="1703270at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11380">
        <property type="entry name" value="Ribosomal_S8e_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.168.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.168.20">
        <property type="entry name" value="Ribosomal protein S8e, subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001047">
        <property type="entry name" value="Ribosomal_eS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018283">
        <property type="entry name" value="Ribosomal_eS8_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042563">
        <property type="entry name" value="Ribosomal_protein_eS8_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022309">
        <property type="entry name" value="Ribosomal_Se8/biogenesis_NSA2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00307">
        <property type="entry name" value="eS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10394">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01201">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01193">
        <property type="entry name" value="RIBOSOMAL_S8E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ83749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="200" mass="22364" checksum="230E472407568FEB" modified="2017-01-18" version="1">MGISRDSRHKRSATGAKRAQFRKKRKFELGRQSANTKIGAKRIHTVRTRGGNKKFRALRVETGNFSWASEGVAKKTRIAGVVYHPSNNELVRTNTLTKAAIVQVDATPFKQWFEAHYGQTLGKKKTASAEEPVSRSKSVEKKLAARAKDAKIEYSVESQFNSGRLYAAISSRPGQSGRCDGYILEGEELAFYLRRLTAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAA5</accession>
    <name>A0A1E5RAA5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Anthranilate phosphoribosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2717</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAA5"/>
    <dbReference type="FunCoup" id="A0A1E5RAA5">
        <property type="interactions" value="317"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAA5"/>
    <dbReference type="InParanoid" id="A0A1E5RAA5"/>
    <dbReference type="OrthoDB" id="427800at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004048">
        <property type="term" value="F:anthranilate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005940">
        <property type="entry name" value="Anthranilate_Pribosyl_Tfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01245">
        <property type="entry name" value="trpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285">
        <property type="entry name" value="ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285:SF2">
        <property type="entry name" value="ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 3" evidence="3">
        <location>
            <begin position="109"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ83840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="386" mass="42352" checksum="4DEB0F9BA0601B3D" modified="2017-01-18" version="1">MSEFTKNLSVHTKSILNGTFVPQNLYDCLIDIVTYLSTTKFSPVNEDEKLQSYVNISSFLTALRSKELDIKAEYIASAAKAILTFSNVVQTERIFKDLTSNSTQDEQEIVADIVGTGGDGQNTFNVSTSAAIVVSGIPGIKIVKHGGKASTSNSGAGDLINHLGVVSAKVHSANVEKHLYHKNKFLFLLASQFHNGMFHLAELRKLMKIPTIFNVLGPLLHPVDLVNKRILGVYSEKLGQEYAKASKLIYPNSNTMVVFGKCGLDELSPIGETKIWEVSPDSNEILTFQLSPQDFGLQEYPLSQCLSEGPKENAEFLENKILKGAFHKGENPIYDYILLNSALLYCLCTNTKDYKNAVAIVEESIHSGAALSALHSFVASVQELEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAG9</accession>
    <name>A0A1E5RAG9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein disulfide-isomerase MPD1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2778</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAG9">
        <property type="interactions" value="213"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAG9"/>
    <dbReference type="InParanoid" id="A0A1E5RAG9"/>
    <dbReference type="OrthoDB" id="10264505at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45815">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45815:SF3">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184676" evidence="2">
        <location>
            <begin position="22"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="3">
        <location>
            <begin position="2"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="335"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ83910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="379" mass="43186" checksum="6872392FC798B2CA" modified="2017-01-18" version="1">MVQAXFLFMSILALFFQTVHGQNFYSKNPNIMELNLANFDRVIHQTNYTTIVEFYAPWCGYCQQLKPAYEKLGKTLKDVAPVQVAAVNCDLQLNKXLCAENKIESYPSLVVFRPPKVVLDNNADIGKGRRNKKSAPKQTRTISKHASELYKGERTFRSIKDFLLSRVKNYIRKHIRLEKLYKTVTSLENKRPQVLLIGKDAQIPLLLKSLSIDWLDVIDYHMIPWSKLKTPYCPTKKTGGINSDIDTFFCETVTSLDQDESYLVAFDLKNDKVSIYNSSTGSIEDQNAEKDKPSNNRLKMTNWLLKMYDGLKPMEGLTSLRHKYLNNQYLGIKSKKSKSKSNKLSSTTSPDPSSTTPVYSSKGEASSSKAQDEVQHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAQ2</accession>
    <name>A0A1E5RAQ2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2601</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAQ2"/>
    <dbReference type="FunCoup" id="A0A1E5RAQ2">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAQ2"/>
    <dbReference type="InParanoid" id="A0A1E5RAQ2"/>
    <dbReference type="OrthoDB" id="5378975at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="373" mass="43629" checksum="0E31F3B75FC4454C" modified="2017-01-18" version="1">MLFITRPISQKSNVKNFIEKFEKYGKQDTIQDTIQNNGSNESVNEIKVAPIKEKFEQGIRNNEKETNIMEQETSDIIEQEPNDIAKNINETEQEPNDIAQNINETERSENKSVQSEDDDFGNFSDASIDEDLNVNTSQFETPRLKNTNCDEQINSIIELMFNDTNNPEFTHEAEVLESQQSNHCDINSLLSPRSRIIYEQLVCLPIQEEPPLLWSNSYMRVLLYNILNIKDHDKIKHEELNTSPSKISTLETNTPINSTMFNQITELLNIKIQHHVPETEKFEEPVPKFNINEMKTELLQEIRNLSNNKELLELSSELIDIKLTHMELLKDKQTYEQVINNLVGHTQRLRRNEIAKYNKKHHRQLKRNSLWPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAS5</accession>
    <name>A0A1E5RAS5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Glutamate--cysteine ligase</fullName>
            <ecNumber evidence="3 10">6.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">Gamma-ECS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8 10">Gamma-glutamylcysteine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3125</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-cysteine + L-glutamate + ATP = gamma-L-glutamyl-L-cysteine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13285"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glutamate--cysteine ligase type 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.2" evidence="3 10"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAS5">
        <property type="interactions" value="373"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAS5"/>
    <dbReference type="InParanoid" id="A0A1E5RAS5"/>
    <dbReference type="OrthoDB" id="7939818at2759"/>
    <dbReference type="UniPathway" id="UPA00142">
        <property type="reaction ID" value="UER00209"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.50:FF:000006">
        <property type="entry name" value="Glutamate--cysteine ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.50:FF:000002">
        <property type="entry name" value="Glutamate--cysteine ligase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.710">
        <property type="entry name" value="Glutamate cysteine ligase subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004308">
        <property type="entry name" value="GCS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11164">
        <property type="entry name" value="GLUTAMATE CYSTEINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11164:SF0">
        <property type="entry name" value="GLUTAMATE--CYSTEINE LIGASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03074">
        <property type="entry name" value="GCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="4 10 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ84006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="670" mass="78098" checksum="2F93C58CF67047A2" modified="2017-01-18" version="1">MGLLSSGTPLPWAESRAYNEHVRTNGIEQLLHIFKAAAGRKDDRLLWGDELEYMLINKDNGKLSIKDDDILELLETLLESKLETTDDQXEKRNSNITYHPEYGRFMLESTPRAPYNLTKEYSAYSYVEKNMAMRKEYAKEELDKRNIVASSLTVYPRMGCKNFLDGYDDLIFTEKNTSSQSLFLPDEVMNRHPRFPTLTANIRERRGEKVCIQLPMYIDENTPETDDSVYERNWFKHDSEVFHILKKNHVYMDSMGFGMGSSCLQVTFQAPDLSQARFLYDSLNIFTPVLLSLSAAAPYFKGFLTDQDIRWNVISGAVDDRTPHERGVPSIFPGNENGGTTAKSNSFKGHIPKSRYSVVDLYLGGHPHFDESLNNTDVPINDKVYNRLINNEIFPMDANLARHFAHLFIRDPLVIFQEKVDIQNNTTDTDHFENIQSTNWQTLRFKPPTQAATPGNLKVPGWRVEFRPLEIQITDFENAAFSVFIYLLVECILTFPXSINTYIPMSEIWENMDTAHHRDSVLKSEFYWKSTFNDKKNFETQKASMTNIFNDKEYGIFNVFINRILKHKQLIANDWSELLEKHSSNKDLVRLYYYLYYIHQKSVGKIPSWALYLRNFVLNHKDYKKDSKVTEIINRDIIHLTEXFYHYSNKDLLVXFFGQRLGSYLFENPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAX6</accession>
    <name>A0A1E5RAX6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Actin cytoskeleton-regulatory complex protein SLA1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Actin cytoskeleton-regulatory complex protein sla1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AWRI3579_g2613</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Component of the PAN1 actin cytoskeleton-regulatory complex required for the internalization of endosomes during actin-coupled endocytosis. The complex links the site of endocytosis to the cell membrane-associated actin cytoskeleton. Mediates uptake of external molecules and vacuolar degradation of plasma membrane proteins. Plays a role in the proper organization of the cell membrane-associated actin cytoskeleton and promotes its destabilization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Actin patch</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SLA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAX6">
        <property type="interactions" value="169"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAX6"/>
    <dbReference type="InParanoid" id="A0A1E5RAX6"/>
    <dbReference type="OrthoDB" id="26539at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000147">
        <property type="term" value="P:actin cortical patch assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09532">
        <property type="entry name" value="SAM_SLA1_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11773">
        <property type="entry name" value="SH3_Sla1p_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11775">
        <property type="entry name" value="SH3_Sla1p_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000300">
        <property type="entry name" value="Actin cytoskeleton-regulatory complex protein SLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.700">
        <property type="entry name" value="SLA1 homology domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056996">
        <property type="entry name" value="PH_SLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007131">
        <property type="entry name" value="SHD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035800">
        <property type="entry name" value="Sla1_SH3_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035821">
        <property type="entry name" value="Sla1_SH3_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735:SF19">
        <property type="entry name" value="ACTIN CYTOSKELETON-REGULATORY COMPLEX PROTEIN SLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735">
        <property type="entry name" value="FCH AND DOUBLE SH3 DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24081">
        <property type="entry name" value="PH_SLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03983">
        <property type="entry name" value="SHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="11" id="KW-0967">Endosome</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 17" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="19">
        <location>
            <begin position="3"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="19">
        <location>
            <begin position="69"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="19">
        <location>
            <begin position="362"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="133"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="440"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="650"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="714"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="850"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1215"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="171"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="188"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="199"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="448"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="514"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="531"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="550"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="565"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="665"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="684"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="714"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="746"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="1216"/>
            <end position="1238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00070651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OEJ84045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1254" mass="137611" checksum="79790100D63061F3" modified="2017-01-18" version="1">MTVFLGVYKAVYAYEPQAAEELKIDEDDILYLLQKSDVDDWWTVKKRVIGSDAEEPSGLVPSNYIEEMAPIGQVKALYDYDQVQNPEEELQFHENDLFDLYDENDPDWLLCKNLTDKQYGFVPGNYVEKIDGSAGGAAPSTTTPIATAPPAISNPPASFLPPPVRLDREQYSSTSLAEEAQKQTAHVSPEDEHNKRTEVPPPSSYSPPPPTQPTRPIVDEDDYSVEMSPPRQPTRPTNTQASKSKDFSKFKSWKVNEVVGRKKLKANLSIGNNTIVFSSTNSPDDQPEQWDIKDLITYSNEKKHLFLDLSNPPASLELHTGSTDVANDIMRVLGEVKGGATSQMLNEVKEASNVDAFKNKSSSLKQGKVIYDFIAESPDELTIKSGDIVYILNDSNSNDWWLCELANKRGTKGVVPAQFIKPVNQLSSLAQGGGEDADDYYLDNKDNKSKHHNHKDSKAKKLFRSLTGRKSKYTTEESDEENDTRSRSNSRSAAKDDYFSGLPTKATSSGPSGNNNYDSYDNSNNSYNNDFSKKEEDEHLSTGQKLKAQLTGSWKSDMTNTSKKNSSRKRSASTSSKKKKDKEYPNAEKVRTWVDKSGTFKVEAEFIGCSQGKVHLHKINGVKIAVPADKLSLGDVEFVERATGFSLDKYKDQKPLQDSTMSPSKNDDAHASRDRERERRRKAREREERXRDRSLREQELDELRRARDMLDEERRKMRAEKERAREKDLPPVKPPRPDQQQQQQPLSSSRTGAHSNSSKPPYDWFEFFLNCGVDVNNCQRYTINFEREDISEDILPEVQPSLLRTLGLKEGDVLRVMRFLDNKFGRVSAQLTGAPTGGLFTEADGSLKVNSSTPATALPAQQPSSGATQTQQPVLDEDAWTVKPAANNNLSGQKSAQFSGSMQDLLDLEPLQSTASPTPALLKDLKPVATSSAPALTPSLTSNNTGKSIGVTKTGNALNPDDLIPLNPFKTGGNNVLPMYMMPVMTGGPLMMPMSTTGGAMMPQTTFGAGLQPVGTASNGGFILPVQKTGNGLVPANFTGGTAPFPQTSFGTGLQQGLTGNNVMQMPQLSFGQPQMTGGAMPQNTFGQPQXTGGAMPQNTFGQPQMTGGAMPQNTFGQPQMTGGAMPQNTFGQPAFTGGAMPQNTFGQPQMTGSILPQQTTNGFIPQSTFGMTLQKTGGVAPLPQTSFNSFNQPQTNSLNSAMQNMNIYNHQQPLQNQPTGFGFGNGPQQQPMQQQQTGRANLFNASADNPFGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB47</accession>
    <name>A0A1E5RB47_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ran-specific GTPase-activating protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2858</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RB47"/>
    <dbReference type="FunCoup" id="A0A1E5RB47">
        <property type="interactions" value="183"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB47"/>
    <dbReference type="InParanoid" id="A0A1E5RB47"/>
    <dbReference type="OrthoDB" id="411251at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000454">
        <property type="entry name" value="Ran-specific GTPase-activating protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138">
        <property type="entry name" value="RAN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138:SF142">
        <property type="entry name" value="RAN-BINDING PROTEIN 3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RanBD1" evidence="5">
        <location>
            <begin position="220"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="175"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="7"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="95"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="120"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="175"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ84101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="359" mass="39467" checksum="DF3A52004CAC3390" modified="2017-01-18" version="1">MSVQQQDAKEDKSNTIKESQAKPKADVKPEQLLEKNHKKRPHEDGKADTDNGDQLKDNNSEHDVVTKKKPHLDKLSDPLVNDTKEDTSKQPKIAFGASSPFSSGFGLSTTGQKPFGSFGSFGTVQSPNAFLQTSSSEFNSNNDGAVNSSKTETKTFPSFGASMSFGAGFGVLKKSEEDGAESKNKTEEESVKEEDKKEEGNTQETTKEKKEDDKEDSKSSTKEVSIKPSEAQLTAQEVKSGEEDDIIKFQSNAKLYELIDIKEGWKERGVGPLHVNESKESGKSRIIMRNKALLNVILNLPLIKGFQVHKGFPGSVQGDKFIRIVAVNENQKPVQYAIKVGRVESIDELVECINAALQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB94</accession>
    <name>A0A1E5RB94_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Serine/threonine-protein kinase STE7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2943</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="3971161at2759"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:MAPK cascade"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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    </dbReference>
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        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE DSOR1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47448:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE STE7 HOMOLOG"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="288"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
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            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
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        </location>
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        </location>
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        </location>
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        <source>
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        </source>
    </evidence>
    <sequence length="786" mass="88080" checksum="ECA1E160732EB6D2" modified="2017-01-18" version="1">MNETKQGGSMFKAKSLSSRANFKKLTLNNSGNGSNVPATSSVRTSSLNDSNEYEYKVSSSNDNYKPSNNNETQTHRRLPLPTKKAYTSSVSSSSSSFSSNSSSSGHINPSQPLANTSSQSTNKPLFQKSLNRLNKPKLNLFGNNKSASTGDGSSSGSGSGSGSGSGSGNNNNNNNSNSNGFSFSNFKNISPLKHKQDYITAECGSITPTENNHFSNSSSPSSLISSYAHHDGYINDKDEIPTNNAVDIDNFNKLNISNEDKHLSQTENVSLRNTIKNQNEPEIQLEDIVQIGKIGSGNSGTVLKVLHVPTQKIVAKKTIPLNSIFSSTDNNNKDIMLQVKKQIVRELTIMQKIGKHNNIVEFYSAFYNTITNKHTKLVTPLSTLTIELNNSNNSNNSNNSNNSTNDNGTFTSQANNTLSSLSRRNNGTFDGTDRNFGITKQRIEIEQVCESDTNDKSINDKQRSQDDDDDDDDDKRITNEIIILMEYMNCGSLDRILSTYKKHCKRCGVAVCPETSWFNNENYISKISYGVLNGLYFLYRNFKIIHRDIKPSNILINSKGFVKICDFGVSKKLVNSIADTFVGTSTYMSPERIQGGIYTTKGDVWSLGLMIIELLSGRFPLSSNKFKRPTNIKRKITSKRYDGDLIIEEEEEEEEEEKEKEEEGFQQGFAAEEEDMDDTPDGILDLLQRIVNEPSPTLPDYNNMYSLDLRDFVNKCCIKDEAERASLKELLEHPLILKYNFNNDTERQQKYDYEFKRWCKNIKKLAHEEKLMQRKDQMQALNFGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBE2</accession>
    <name>A0A1E5RBE2_9ASCO</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2471</name>
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        <name type="scientific">Hanseniaspora osmophila</name>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84212.1"/>
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        <property type="term" value="C:anaphase-promoting complex"/>
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        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
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        </source>
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        <source>
            <dbReference type="EMBL" id="OEJ84212.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="374" mass="43190" checksum="5C696640DA542716" modified="2017-01-18" version="1">MEDLCLRSMFSSLIDNXLKPASSTSNGIQGPNIFERYLDSQQXAESNGTKTNATRMFGLVNXQNTKPNKTKKHTTKISNRNATNRRRKKREFISLDSYVPSVQGPIEVGNAKRCLQKNEEERLKIRLLGINSFVPIGINKTLGTILQEREEEKQQMEAAREEEEQFQRQLQEAQQMQQTHHAQEVELEEEEEEEEQQFRELQXVASNHTTEYFSRTNESTTNSDVNMNIDLDEELQEEENVSYGFDEEEADEEEELNIYDQEVEDSTADFEQGEHEGDFEEHEGDFEEHEGDFEEHEGDFEEHEYSRVHXSSESSGHMRSGHVLDSNPDLTHEFESFSRDTHSLRVNRSGDSNSNNGFGDFLLSEPSMFPELGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBF0</accession>
    <name>A0A1E5RBF0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SWI/SNF chromatin-remodeling complex subunit SNF5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2503</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
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            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84225.1"/>
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    </dbReference>
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    </dbReference>
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        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006939">
        <property type="entry name" value="SNF5"/>
    </dbReference>
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        <property type="entry name" value="SNF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04855">
        <property type="entry name" value="SNF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="73"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="247"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="885"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1107"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="16"/>
            <end position="32"/>
        </location>
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        <location>
            <begin position="73"/>
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        </location>
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        <location>
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            <end position="119"/>
        </location>
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        </location>
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            <end position="151"/>
        </location>
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        </location>
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        <location>
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            <begin position="334"/>
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        </location>
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        <location>
            <begin position="889"/>
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        </location>
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        <location>
            <begin position="1111"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1142"/>
            <end position="1162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ84225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1182" mass="132775" checksum="C6684FB3FFC810C6" modified="2017-01-18" version="1">MNNNPDNGNASNPSSHDMNNPQNPFSLLQNIGTPGFVIPPQLLQSLTPQQLHLIQQKHQQLLMQRQLQQQQQPQQQQQQQQQQQQQQPQSFAQHPQTMQPMSQNSPMQSPAVSAPNTNGAGIAGSDYRSNIYNGSPAPSVSQVGSERSSSVNGNPNMNNSIGEITQLPPQFRNLPPQMQQQVLFQLNQKKMALQQQQQQQQQQHYMQQQAQLRAQFQQQQMAAATGKGDAXISTDKNSFTGALQNMGQSRMPASKAGASKTNTKAAPKAAGTKASVANKTGIMKQAPVKTAAGRSNMGRGGLKTTGAKRQTKKQQQQQQQQQQQQQQQQPPQNVFPSSLPTTGIESMSPVESAKPALXSAELTPADFEDIKNRLPKLPPLPKYTIVTQEPELVKLQNPAKYWSSRLKHELHENGSAELMXYEQIVLKDCEMLRQISKERDGVSLNSKYGQSIKEYTGRFSSDLHYYQEVKKTRLETIENVKQNKPTKSIWGHGFLGYGNGFTDTETKVISGDQNLTKSKRTLFYDDISKLYNQAMNETQESLVPIRLDFEMEKDKFQLRDTFLINKNDDMTKIDEFVIEMLKDYKFKNYQEIYPVISNSIKEQISEYQEDPFAKFNHLKSYSSVASVNNNINNNNTVSNTVSTSGNKTMSKICQFVGDDIRIKINLDIIVGSTELVDILEWDISNPENSPEEFAQEMCKELELPGEFVTAIAHQIREQIHMYHKALVLSGYQFDGNFVEDDEIRSKLLPLITLDNVLRNSSDLRNYTPNVFNITPAELDRLDKDKERERDTRKRRRANATKINYNVKRHRSTNTTHSNVSEHLNDNGNGASNTSLFSMGNINNSGSNTSLLNGALQQIASGVSLSSMLDGTTDLLSFNTPLLRDENDDLNNNSNNNNNNYNNNSNNSGDNTAGGLNLFKTPASVPNIFNNTLNFSGDFYSGLGNNDDKNDDNGVDIGMNFIGTVTNSIISGLNQEDIDDLKPDLNEIPKTFRTLVPSTILPGGYDLGPNVQSFTTKYEKKYEKRPDPFVYILRNNDMFGNKGKQETNLSQENLIGVEENNAKYLIKIKFKKDECKRNLKTCNALIVAKIKLELKTREEEKIKANLNSKTLEEEEEEEEEEEEISKQRPTFAVKQPVMGFENMGSNVNDEISAGTSSVSNSELNEPAPSNEDIFRVNNETIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBN7</accession>
    <name>A0A1E5RBN7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Aldehyde dehydrogenase 5, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2866</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBN7"/>
    <dbReference type="FunCoup" id="A0A1E5RBN7">
        <property type="interactions" value="940"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBN7"/>
    <dbReference type="InParanoid" id="A0A1E5RBN7"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019413">
        <property type="term" value="P:acetate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000001">
        <property type="entry name" value="Aldehyde dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000001">
        <property type="entry name" value="Mitochondrial aldehyde dehydrogenase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="5">
        <location>
            <begin position="68"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ84314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="535" mass="58943" checksum="B6E6534FDC5FD719" modified="2017-01-18" version="1">MLRTKTQSLFKTPSTRPFVQGLFNNNATSSSSATLFMRLKSSLYSPITLPNGLKYTQPTGLFINNEFVESYHGKTFEVINPFNEETITKVQEAREQDVEKAVSVAKHVFETEWKNSKPEFRAQCLNKLADLVEENAELLASIESYDNGKALSSSKIDVELVAKYLRYGAGWCDKLTGKVMQNQPGNFSYTVREPLGVCGLLSSFNFPLMFVSWKLVPALVTANTCVFKPSEHTPLSTLYFGELIKKAGFPPGVVNFLPGFGKYCGNAICQHKDVKKVSFTGSGPTAEKILQTCGIKKTTMELGGKSASIITKNAKDLDKVVANVALGIFFNSGEVCCAGSRVYVQEDIYDDFLNRLKIYAENLKVGNAFDDPFYGPLTNKAQFDKTMHYIDVAKSSDKATLLTGGQRIGNEGYLVQPTVFSDANNEMRFVKEEIFGPVLAVSKFKTVEEAIKMANNTEYGLAAGVFTDDINEALDVSSRLEAGTVWVNTYNDFHPSLPFGGHAMSGFGSEMGEEALHCYTAVKAVKINFDSSLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBP0</accession>
    <name>A0A1E5RBP0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Topoisomerase I damage affected protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2864</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TDA2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RBP0"/>
    <dbReference type="OrthoDB" id="10059120at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21457">
        <property type="entry name" value="DLC-like_TDA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.40">
        <property type="entry name" value="Tctex-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038586">
        <property type="entry name" value="Tctex-1-like_sf"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ84315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="118" mass="13164" checksum="254820BAF1839767" modified="2017-01-18" version="1">MPNISTNNHNIDNAPVQPNKIIPMVQDIFQENGYEXGVCCEKLTQSVSEMSSKHKFIVNVTEVKLANKDQNVSDICVNNSFGTNWDNDKDGVVNCSVIDGEGGRLFMVSVFWINEQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBR7</accession>
    <name>A0A1E5RBR7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Increased rDNA silencing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3107</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the IRS4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBR7">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBR7"/>
    <dbReference type="InParanoid" id="A0A1E5RBR7"/>
    <dbReference type="OrthoDB" id="10045710at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000326">
        <property type="entry name" value="IRS4p EH domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EH" evidence="4">
        <location>
            <begin position="1258"/>
            <end position="1351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="51"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="180"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="486"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="559"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="665"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="822"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="902"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="941"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1116"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1379"/>
            <end position="1398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="127"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="200"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="255"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="285"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="299"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="382"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="489"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="559"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="579"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="678"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="825"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="871"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="908"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="963"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1001"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1025"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1132"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="5">
        <location>
            <position position="835"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00061579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ84344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1398" mass="155661" checksum="224987788A2F5DCC" modified="2017-01-18" version="1">MKASVTQKRHNPPSSSSVRRLQKRDKTTEEMTQEGIIDSLTAAKAIFAKYHPDSMVDKQPQRTNSVVENRFRHTQSQRPLKNPGLQRPAPKSSNNSPYIIKTPPANVGIKKTPTLAKSNGLQAKQFSKTESKKRVKSETQHLNGLPIAPKQIKTHNALSTPQKTIPVKKTAKQQFIASPRLKSLSQAQSPSALSKSPASNLISRHSSGHNSIXSVRTVSTNGTLTKTPVFASPNASPSVKSNNSSMATIRSNSIKPSQKPSQKPKPLTIGSPPTKVQKKPIAHTHPVDTSRSNEKDHGNNLFSNFSSSSDNYTPSTASSSQRFSDSDTSFIQHKKAGSGSLKPKREEKHRTLTRKLNTKNTLDEANSGHHTTTKHKSRNXYESTQSSTPESXSSDVGAAVAASKALYTLPGYDSMNSSVEPSKKDKVHHHGLRSKLFNFRRDPKMRNSLDSNNFGDTFSISSAAQPKNLNMDHARKSKRHSLDLYRAGSMTSQRPNDSTLSFPKPSTSFGSFSQNRESKIHQLQKKIEEEKKPEHKTVNMRTVAKINLLADEIARKTIESSKNDSHEGVNKTNLSLKMPSATSSPKKTISTSPSFNGDTTMLSPLSIPDTPLAGFASRNGSKSSLLQNFRPNTSQPSQAEAHLLNNFVPHLNSFSQTNNTSSFYGTKNLKRIPPPPSSMASSQSLASSFDHLSPSAPGSVRSSFDMNNYSSESLANLEPALTIKKNGESDKAGNLMGSNDQISYNEDXELNSIMHSQYDHPNIDEENSDVVSFVKSDKSNNTLENFPQEKKGLEFVENERDXEVKSSSAIFGRAMRSLTNFSLHSDSKDERARADDHXKRNRTGTAVSNAATIVVPQHAGRSADSSPPSPQTVQPPQSSASSIVSSEKKKSFGPFASLIRRVSSGHHGTADKNGPKSEEAEQQQLPAPVQSNSHNYQYEKQIPHYQQVPQTKILTTLREDKPKRSKKEKLKKAFMLSGHSGQQKHAKNSENDSNQKRGSSRDQAMSLDNNTGEHGRKDSANGNGSDEDDDDDDDEYDYLYYKTNQAMPHRLYRYKDDDDEDEVYKKKKMHLKSLIKTPAKFTYDSIKKSTNKLNDMTALNHNQSHNKVNNSDNLSQLEEAAENESPSRSETFDQSEQASDNILQSPAVSTSGDASDNFEDTYSTLAGDFDESNTSSRLSLDGTPAKNTFNDSFVMPVGTSHPDMEMYMVRGRGGNKKIKNKAFDADKPWKNHVDLGYITPQEKKRYEGIWVSNRNSYLSLLPPSWKSQYENEVIEEGDEDVILNILVYEIWSRSHLSPKLLRQIYNMVDLYKDGLLRRRSFIVGMWLIDQCLYGKKLPPKVDDRVWQSVDKIIIGVNIDAKSFAKKKKKYIRKQLKDLKKQEKQHNQKLMLARQGKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RC11</accession>
    <name>A0A1E5RC11_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Threonine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="3">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Threonyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g2932</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RC11">
        <property type="interactions" value="1019"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RC11"/>
    <dbReference type="InParanoid" id="A0A1E5RC11"/>
    <dbReference type="OrthoDB" id="5423599at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01667">
        <property type="entry name" value="TGS_ThrRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00860">
        <property type="entry name" value="ThrRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000009">
        <property type="entry name" value="Threonine--tRNA ligase 2, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000003">
        <property type="entry name" value="Threonine--tRNA ligase 2, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000006">
        <property type="entry name" value="Threonine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000005">
        <property type="entry name" value="Threonyl-tRNA synthetase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047246">
        <property type="entry name" value="ThrRS_anticodon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF46">
        <property type="entry name" value="THREONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGS" evidence="15">
        <location>
            <begin position="81"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="14">
        <location>
            <begin position="325"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ84113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="741" mass="85224" checksum="469EF4699110C4D9" modified="2017-01-18" version="1">MSVEEQVKDLSLEDKQKQQAQPTKDQKPKVNKKEKKAKKAGASGALVSSLYLDPEPKFIQDRLDFFSELKTKYDAEVASKXRLPIKIVLKDGSIKEATSWETSPLDIAQGIAKSFSERQCISRVNGELWDLNRPFESETENEEIKLEFFDFESEDGKKVFWHSSAHVLGEACECNLGAHICLGPPTDDGFFYEFSVKDSLDEKTEGAAVSQADFPNLETVAKKVIKEKQPFERLVMSKEDLLEMFKYSKYKKYLISTKIPDGSSTTVYRCGKLIDLCTGPHIPHTGRIKAFKLLKNSSCYFLGESTNDSLQRVYGVSFPDKKMMDAHMKFLEEAAMRDHRKIGKEQELFMFNEMSPGSCFWLPHGTRIYNGLVNLMRSEYVKRGYQEVITPNMYNSKLWEXSGHWGNYKENMFTFDVEKETFGLKPMNCPGHCMMFKARERSYRELPWRVADFGVIHRNEFSGALSGLTRVRRFQQDDAHIFCMPSQIKGEIADIFDFLNVIYGIFGFDFKMELSTRPEKYVGEIETWNNAEATLQECLESWGGDWEINAGDGAFYGPKIDIMISDALKRWHQCATIQLDFQLPNRFDLEYKSKSDEQYERPVMIHRAILGSVERMSAILTEHFAGKWPFWLSPRQVLVVPVGVKYIEYAEEIAKTLTLEGFYADADVTGNTLQKKVRNGQMLKYNYIFIVGEQELNEKSVNIRNRDVAAEQGKNETIAFATVLEQLKNLKDEKRQDNILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RCS8</accession>
    <name>A0A1E5RCS8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">beta-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g2850</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RCS8"/>
    <dbReference type="InParanoid" id="A0A1E5RCS8"/>
    <dbReference type="OrthoDB" id="47059at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000495">
        <property type="entry name" value="Periplasmic beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050288">
        <property type="entry name" value="Cellulose_deg_GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011658">
        <property type="entry name" value="PA14_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715">
        <property type="entry name" value="BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715:SF27">
        <property type="entry name" value="BETA-GLUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07691">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00758">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PA14" evidence="6">
        <location>
            <begin position="413"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="7">
        <location>
            <position position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ84383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="840" mass="92785" checksum="75D72A1A6B29514F" modified="2017-01-18" version="1">MTVDFDVDKLLNELTVQEKIQLLAAKDFWHTYPIERLNIPSIRVSDGPNGIRGTKFFDSVPSACFPNGTALASSFNEDLLVRAGELMALESKHKGAKVILGPTTNIQRGPLGGRGFESFSEDPYLSGVATAAVVNGIQKSNEIAATVKHFVCNDLEYERFSSNSIVSERALREIYLEPFRLAVKXSDPKLFMTAYNKVNGIHCSSSKKLIQNILREEWKSNATVISDWYGNSDIVDSIQNGLDIEFPGPTRFRDPEIVQHLLGCKTETREGGQFSIADIDARARKVLELVKYFVDVEGTTELPTNEDDKNNTPETXKLLREIGNETIVLLKNDKNVLPLNKKDDIVVIGPNAKAKNPSGGGSASMNGYYTVSPLEGIAKAIGKNVDEIPYIKGCDNHKTLSNLIEQCTNDADPKRKGAAMKFYTKSRDSRGDEKPFDEYVVDKSFITLFDYKHEKISSKEKLFYCTVEGYFTPEESGDYEFECQVLGTALFYIDDKLVINNKDDQTAGNFGFGSGTAPKNNIVTLEKGRKYKIFVDYGSGVTSKLSQSIAAGALQIGVNKVIDAEAEIKKAAELASKHDKVILVIGLNGEIESEGYDRDNMQLPRRTNDLVTAVLKANPNTVIVNQSGTPVEFPWLQQATTLLQAYYGGNELGNSIADVVFGDANPSGKLSLSWPLKNEDNPAYLNFKTVMGRVLYGEDIYVGQVAYPFGHGLSYTTFKFNELDVSGDDESLKVELSVANTGKVDGKEVVQVYVARTSPSAVPRPVKELKKFKKVALKAGESAKVELTLSVKDSCSYFDEFHNQWHLEAGKYQVLVGSSSDDIHLIGDFEVKESKFWLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDI6</accession>
    <name>A0A1E5RDI6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein mS29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1888</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDI6">
        <property type="interactions" value="122"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDI6"/>
    <dbReference type="InParanoid" id="A0A1E5RDI6"/>
    <dbReference type="OrthoDB" id="274828at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019368">
        <property type="entry name" value="Ribosomal_mS29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017082">
        <property type="entry name" value="Ribosomal_mS29_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12810">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12810:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10236">
        <property type="entry name" value="DAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036996">
        <property type="entry name" value="RSM23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="unsure residue" description="D or N" evidence="8">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ84965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="424" mass="48218" checksum="9B9AAB733463ABB9" modified="2017-01-18" version="1">MLRQSCRSFHGSATINAATASKAFSKKGGAYQPRKKRLTPNCLYKPFEQTASTSKFNLKAPKLDNEVFVPQDVKKSINTVTEYGSEHLKALYNMGTFKQGQHNELFRSPISLIRETSTLEFLEKLKKHSKFVITGEQGIGKTTLLAQTQSALSDTHIXIHVSHPAMFLNGQTDFHFDQSLNEYTNPMFIQDLFRKVLRCNEKMLKNIKLSNTHKFQVLDVKNGSKSIKTFEKEKNSLYDLISIKTSSESKGKLFQQFIHELQHQKEYPVAFTMDNFSRLVTTSYTSYRDTEAHHIPTLKFQLPRMIFDIISGDISFAHKXSKVVLAVTGGHRSNKTLPTALGTYERNPYLSLYQYNEEFAAKMKKGGLQAFEVPRLSSNEVSILLDYYKKAGVSNQDHSAKEEYFVSGNGNPLALIKSITMNFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RE88</accession>
    <name>A0A1E5RE88_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Actin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2349</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RE88"/>
    <dbReference type="FunCoup" id="A0A1E5RE88">
        <property type="interactions" value="1437"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RE88"/>
    <dbReference type="InParanoid" id="A0A1E5RE88"/>
    <dbReference type="OrthoDB" id="5132116at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10224">
        <property type="entry name" value="ASKHA_NBD_actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000291">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000001">
        <property type="entry name" value="Actin, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000404">
        <property type="entry name" value="Major actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00406">
        <property type="entry name" value="ACTINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00432">
        <property type="entry name" value="ACTINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="360" mass="40179" checksum="7EF0D9C6BAF0C34A" modified="2017-01-18" version="1">MCKAGFAGDDAPRAVFPSIVGRPRHQGIMVGMGQKDSYVGDEAQSKRGILTLRYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPMNPKSNREKMTQIMFETFNVPAFYVSIQAVLSLYSSGRTTGIVLDSGDGVTHIVPIYAGFSLPHGISRIDLAGRDLTDYLMKILSERGYSFTTTAEREIVRDIKEKLCYVALDFDQEMQTAAQSASIEKSYELPDGQVITIGNERFRAPEALFNPSLVGLESSGIDQTTYNSIIKCDVDVRKELYGNIVMSGGTTMFPGIAERMQKEITSLAPSSMKVKIIAPPERKYSVWIGGSILASLTTFQQMWISKQEYDESGPSIVHHKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REB2</accession>
    <name>A0A1E5REB2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable endonuclease LCL3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Probable endonuclease lcl3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1925</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LCL3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REB2"/>
    <dbReference type="FunCoup" id="A0A1E5REB2">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REB2"/>
    <dbReference type="InParanoid" id="A0A1E5REB2"/>
    <dbReference type="OrthoDB" id="430293at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302">
        <property type="entry name" value="EBNA2 BINDING PROTEIN P100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50830">
        <property type="entry name" value="TNASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 12" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="11">
        <location>
            <begin position="54"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ85249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="285" mass="32443" checksum="D5D440A75A528B7E" modified="2017-01-18" version="1">MSSDNRQVTWSNPKVILFSLMLTGSILTSLSVFNRYFKRYESAKDIPSHIFKKSFLKGKVTSVGDGDNFHFYHTPGGVLGGWGWLRETPSLIALKSDSPLLIQTKKTSKQKQPFFSSLLHKGPFKLFSTQDPSSSEYFMNLSVPYKNRRGLDTISVRLSGVDAPERSHFGNEAQPFSEEALIWLKHTLIGKTVWIKPLSIDQYGRCVARTVYWNGLFKGGYKDVSLEMIQVGLGTVYEGSEVFAEFDGQEVLYRYHERLAKSRKKGIWGLKKFETPGQYKKRTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REC3</accession>
    <name>A0A1E5REC3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ubiquitin-conjugating enzyme variant MMS2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1927</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REC3"/>
    <dbReference type="FunCoup" id="A0A1E5REC3">
        <property type="interactions" value="1114"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REC3"/>
    <dbReference type="InParanoid" id="A0A1E5REC3"/>
    <dbReference type="OrthoDB" id="6508832at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006301">
        <property type="term" value="P:DNA damage tolerance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23807">
        <property type="entry name" value="UEV_UBE2V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000026">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="2">
        <location>
            <begin position="5"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="137" mass="15314" checksum="73884B0B0E0E4F4E" modified="2017-01-18" version="1">MSKVPRSFRLLEELEKGEKGGSQACSYGLADGDDITMTNWNGTILGAPNCVHANRIYFVSITCGPNYPDEPPHIKFVSKINLDCVDQTTGDVKPEALHILQNWKRSHTMEDILLELRKEMITPNNKKLPQPAEGSTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REF8</accession>
    <name>A0A1E5REF8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">tRNA(His) guanylyltransferase</fullName>
            <ecNumber evidence="2 12">2.7.7.79</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">tRNA-histidine guanylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g2359</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 5'-end ribonucleotide-tRNA(His) + GTP + ATP + H2O = a 5'-end phospho-guanosine-ribonucleotide-tRNA(His) + AMP + 2 diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54564"/>
            <dbReference type="Rhea" id="RHEA-COMP:14193"/>
            <dbReference type="Rhea" id="RHEA-COMP:14917"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:141847"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.7.79"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="14">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the tRNA(His) guanylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.79" evidence="2 12"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REF8">
        <property type="interactions" value="767"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REF8"/>
    <dbReference type="InParanoid" id="A0A1E5REF8"/>
    <dbReference type="OrthoDB" id="62560at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008193">
        <property type="term" value="F:tRNA guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3000:FF:000003">
        <property type="entry name" value="tRNA(His) guanylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025845">
        <property type="entry name" value="Thg1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024956">
        <property type="entry name" value="tRNAHis_GuaTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007537">
        <property type="entry name" value="tRNAHis_GuaTrfase_Thg1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038469">
        <property type="entry name" value="tRNAHis_GuaTrfase_Thg1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12729">
        <property type="entry name" value="TRNA(HIS) GUANYLYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12729:SF6">
        <property type="entry name" value="TRNA(HIS) GUANYLYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04446">
        <property type="entry name" value="Thg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14413">
        <property type="entry name" value="Thg1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028980">
        <property type="entry name" value="tRNAHis_guanylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9 12 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 12 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 12 17" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNAHis guanylyltransferase catalytic" evidence="15">
        <location>
            <begin position="6"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Thg1 C-terminal" evidence="16">
        <location>
            <begin position="145"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="29"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="76"/>
            <end position="77"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00032480"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR028980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR028980-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF14413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ85264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="242" mass="28269" checksum="58525850C9CCEEFC" modified="2017-01-18" version="1">MANSKFEYVRVFETHTTILPETFIVVRIDGKNFHEFSTHYNFDKPNDIRALGLMNAAAKNLVLKYKSEIICAFGESDEFSFILRKDTALYNRRSDKIATLFASYFTAQYIFLWNKFFQTEENPQELDFKHLPCFDSRCVTYPNLKTIKDYLSWRFVDTHINNLYNTAFWKLILXCGLDPKAAEGELCGTLSSDKNEILWTKCNVNYNDEPEIFKKGSLINKKGEILHIDVIKNIDTIFKGFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REK7</accession>
    <name>A0A1E5REK7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphatase DCR2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2078</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07383">
        <property type="entry name" value="MPP_Dcr2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32440:SF0">
        <property type="entry name" value="PHOSPHATASE DCR2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32440">
        <property type="entry name" value="PHOSPHATASE DCR2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="3">
        <location>
            <begin position="333"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="667" mass="75648" checksum="A600F218C85DEB77" modified="2017-01-18" version="1">MPSISRRYRRIAVYITIFVVLPLLLLKYVIIPLKIDSTLLKPSLATSQQVSNFPKTAKTNKDSKNDVEADQKTIKSANNDAKVNEDKNSEKKEGYLSSLTDSIKKKLTEFSLWNYDLKSTFVTDINXISCYYSPLGEACFNKNINPVLFTSDTXSYTVYKKLIKKDINQSKPYHWFGHSKYLVVSYITLEDVLNMQDKYFYAVSGFNADLINTEENTSIKKLDLEGKHEINLVKKKISIQNIGTDFFFSDVDILFGKDCVDPRPGWDLDKKIAIGDSPHPAYFTLKKGFGKSSPLTAGAEETNEMEQNTKIKQHKYFDEDASKRLTLDANGRFKIMQIADLHFSVGSGVCRDQFPVVDKNCEADNETLNFLNIVLDQEKPDLVVFTGDQIMGDECKQDTISAILKVVKPVIDRNIPYAIIWGNHDDEGSLSRLELSEYVSTLPNCLFRLSEYDGVDKSFGFGNYVHQVFTPNGNAAVTLYFLDSHKYTTKPKVFPGYDWIKEEQWDYMKEYYLTKVKPSIENYSRIPLSMAFFHIPLPEYLNLSSKKNPGQQNKIFGSWKEGCTAPKYNSNGIDALVSMDVQVATCGHDHCNDYCLLDDSNEHKNVWLCYGGGSGEGGYAGYGGTERRVRVFDIDTMNGNIYSWKILHESPNTRLDEQLLVNGGIAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REP9</accession>
    <name>A0A1E5REP9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2281</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REP9"/>
    <dbReference type="InParanoid" id="A0A1E5REP9"/>
    <dbReference type="OrthoDB" id="381190at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057564">
        <property type="entry name" value="HEAT_ATR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003151">
        <property type="entry name" value="PIK-rel_kinase_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF125">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE MEC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02259">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23593">
        <property type="entry name" value="HEAT_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAT" evidence="7">
        <location>
            <begin position="1"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="6">
        <location>
            <begin position="416"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ85365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="631" mass="72208" checksum="1A204A41B1FE4C97" modified="2017-01-18" version="1">MSTSEETSIDAVLKFLNKRLAYVGDSFSSKFEIMCVRNSFAKHIKAYNDSFFNMYFDMSRLSKQAHRLDLTSKLLMDSWAQPLRNDFQLEMEYADLLWEKNEKEKALKMVGEIYRNMKADSTKAGNHEEAAVLLKYAQWCDASNSSTPSELITSYKKVCDLDPQWAESYFQLGIFYSKYLGKKVADEKDSKSYVLKKESVKYLLLSLKVSTIHVREALPKVVTYWLDTADSLRTSRDLELRAIVKEMCTIIDKAMSECPVNMWYTVLVQFISRILHSHGESAKRIQKIVVLLALHYPQFILWYVFSMINSSETSMSSCGKAILKKFRASGGSSDFASSAYNLVINFSKICKTAPPNDKTKINTVDTSLEKDFGIDMNIAPSAMAVPVQKNFGVSLPLTQTDKESITSSKXVSISQLGANYAVFASLRKPKKIQLFGSDGKCYEIMCKMEDVRQDDQYMQFAQTVGFLLSKDPESQKRNLGISTYFIMSLHETYGIIELVPNVITVRSMLAKLYQAKGIYEQIRYFHQAWDKVKNDDAKCLELYHRFTNIFKPVLHEWFLNNFPDPILWYSAKQTFSRSYAVMCMVGHILGLGDRHCDNILIDVRSGHVLHVDYDILFEKGKDLPVPEKSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REW2</accession>
    <name>A0A1E5REW2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein OPI10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2257</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OPI10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REW2"/>
    <dbReference type="FunCoup" id="A0A1E5REW2">
        <property type="interactions" value="359"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REW2"/>
    <dbReference type="InParanoid" id="A0A1E5REW2"/>
    <dbReference type="OrthoDB" id="10248398at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048364">
        <property type="entry name" value="Hikeshi-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008493">
        <property type="entry name" value="Hikeshi-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031318">
        <property type="entry name" value="OPI10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12925">
        <property type="entry name" value="HIKESHI FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12925:SF0">
        <property type="entry name" value="PROTEIN HIKESHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21057">
        <property type="entry name" value="Hikeshi-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05603">
        <property type="entry name" value="Hikeshi-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hikeshi-like N-terminal" evidence="2">
        <location>
            <begin position="5"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Hikeshi-like C-terminal" evidence="3">
        <location>
            <begin position="184"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="238" mass="25727" checksum="C46FCD28A780F707" modified="2017-01-18" version="1">MFGAVAAGNPIQLSEEVPNSNGLQHTIVLSSTKPKQYSHITLFQLPNVALPADLVATVYFKLSVDQDFQLFGYLAAEKPSAIFKVTLPGAKGDDSGNLGEIDMDVDGVDTGADSGDVNNISQVLIGISVEPRVQGLAKVQQLKEMNMARASGNAMVLSKNLKNTNPAIPPETPGELAQRFPVVTQQLAANIVQHAYNYLTGFLDPAGNVSIKRFDTWWDKFKVRLANDAHFIDEVTSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF04</accession>
    <name>A0A1E5RF04_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Efficient mitochondria targeting-associated protein 19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g2412</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM97/sigma-2 receptor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF04"/>
    <dbReference type="FunCoup" id="A0A1E5RF04">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF04"/>
    <dbReference type="InParanoid" id="A0A1E5RF04"/>
    <dbReference type="OrthoDB" id="433124at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033118">
        <property type="entry name" value="EXPERA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051987">
        <property type="entry name" value="Sigma-2_receptor-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016964">
        <property type="entry name" value="Sigma2_recept"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31204">
        <property type="entry name" value="SIGMA INTRACELLULAR RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31204:SF1">
        <property type="entry name" value="SIGMA INTRACELLULAR RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05241">
        <property type="entry name" value="EBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031032">
        <property type="entry name" value="TMP_97_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51751">
        <property type="entry name" value="EXPERA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="EXPERA" evidence="9">
        <location>
            <begin position="23"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR031032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ85446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="190" mass="22833" checksum="E924A3B55B948285" modified="2017-01-18" version="1">MVAPVNRDAVPEYKKQYLLSKFADNFYYYFFIIHFFISVFVDSCGVIGIENMYTKNIIEKYISSYNDYLLFERPIWFKNLIYLEIFTQIPMFLWFFTMFNRFYDELNKETFTEEVTKQNKARIHEWKKLIRFFLKVYGLMASASTAYCIYEVYLNGHYPGTAMPLDLNDRLKLISMYAPMVYIPGGLLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF05</accession>
    <name>A0A1E5RF05_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Acetyl-CoA carboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AWRI3579_g1726</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP = N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13501"/>
            <dbReference type="Rhea" id="RHEA-COMP:10505"/>
            <dbReference type="Rhea" id="RHEA-COMP:10506"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83144"/>
            <dbReference type="ChEBI" id="CHEBI:83145"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.14"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>hydrogencarbonate + acetyl-CoA + ATP = malonyl-CoA + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11308"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RF05">
        <property type="interactions" value="841"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF05"/>
    <dbReference type="InParanoid" id="A0A1E5RF05"/>
    <dbReference type="OrthoDB" id="14612at2759"/>
    <dbReference type="UniPathway" id="UPA00655">
        <property type="reaction ID" value="UER00711"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004075">
        <property type="term" value="F:biotin carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042759">
        <property type="term" value="P:long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001295">
        <property type="term" value="P:malonyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.460.10:FF:000001">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000005">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000005">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1770.10:FF:000001">
        <property type="entry name" value="acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000003">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000005">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000010">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.460.10">
        <property type="entry name" value="Biotin dependent carboxylase carboxyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1770.10">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049076">
        <property type="entry name" value="ACCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049074">
        <property type="entry name" value="ACCA_BT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013537">
        <property type="entry name" value="AcCoA_COase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728:SF3">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08326">
        <property type="entry name" value="ACC_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21385">
        <property type="entry name" value="ACCA_BT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0092">Biotin</keyword>
    <keyword evidence="9" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="17">
        <location>
            <begin position="46"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="16">
        <location>
            <begin position="204"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="15">
        <location>
            <begin position="682"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="18">
        <location>
            <begin position="1471"/>
            <end position="1807"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="19">
        <location>
            <begin position="1811"/>
            <end position="2127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OEJ85498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="2219" mass="247671" checksum="EE0D3658EA64D948" modified="2017-01-18" version="1">MTQFEVTDYTAQHKKLASHFIGLNTVDKAKDSLLKEFVKSHGGHTVISKVLIANNGIAAVKEIRSVRKWAYETFGNERIIQFVAMATPEDLDINADYIRMADQYVEVPGGTNNNNHANVDLIVEMAERADVDAVWAGWGHASENPQLPERLAASPRKILFIGPPGSAMRSLGDKISSTIVAQHAKVPCIPWSGTGVEKVNLDESTGLVSVDDEVYAKGCCSSPEQGLEVAKKIGFPVMVKASEGGGGKGIRKVENEKDFIALYKQAANEIPGSPIFIMKLAGKARHLEVQLFADQYGTNISLFGRDCSVQRRHQKVIEEAPVTIAKPDTFAEMEKAAVRLGKLVGYVSAGTIEYLYSHDEDKFYFLELNPRLQVEHPTTEMVTGVNLPASQLQVAMGIPMHRIRDIRMFYGADPKSASQIDFDFSSKAATTTQRKPQPKGHCTAVRITSEDPSEGFKPSSGSISELNFRSSSNVWGYFSVGNKGGIHSFSDSQFGHIFAFGENREASRKHMVVALKELSIRSDFRTTIEYLIKLLETEDFEDNTITTGWLDDLISQKMTAEKPDATLAVICGAATRAFIASEEARNEYVDNLKRGQVPHKNFLQTMYPIEFIHEGLRYKFTVAQSADDRYTLFINGSKCEVGVKKLSDGGLLIALGGKSHTIYWKEEAGATRISIDSMTTLLEAENDPTQLKTPSPGKLVKFLVENGDHITAGQTYAEVEVMKMQMPLVAQESGLVQLLKQPGTSLSAGDIMAILTLDDPSKVQHALPYQGLLPEYGAPIVEGSKPSYRFHRLMTTLENILKGYDNQVIMKSSLQSLIEVMRDPKLPYSEWNTEISALHSRLPASLDEQLNQLTERAFKRDAEFPARQLNKLLEHAVKEQAAVNGLFAGVVAPLLDIAQKYSNGLEQHEYSVFSKFLNEYFEVENLFSGSNVREEDVVLKLRDENSEDLQKVVTTVLSHSRVVAKNNLILAILKHYQPLCQLNANVAAIFTPSLEKIVTLESKSTAKVALQAREILIQAALPSIAERTSQIEHILKSSVVETSYGAGTTKRSEPDLASLKQLIDSNYIVFDVLTQFLNDKDADVVSAAADVYIRRAYTAYNIGDVKHHKVDGSPVVEWKFQLPSEATIANPQAKSKMFMNRTMSVSDLNYVGPTDGQTLRTGILVSAEHLDDVDEALASGLSIIPSNNHASPAPNRSNNHSTLSNVANVFISSTAGFESDKEILDRINEILVMNKQNLIDSAIRRITFSFSFKDGSYPKYYTFRGPNYEEDETIRHVEPALAFQLEMGRMSNFNIKQRFTDNRNIHVYEAVAKNSPVDKRFFTRGIIRTGRIRDDISITEYLKTEANSLMSDILDNLEILDTSNSDLNHIFINFSSIFDITPQDVQAAFGGFLERFGKRLLRLRVGAAEIRIIVSDPKTGAATPLRALINNVSGYVVKTEMYTEVKNSEGVWVFKSLDKPGSMHLRPISTPYPVKEWLQPKRYKAHLMGTTYVYDFPELFKQAVTSAWKKFSPTTKVTDSFFVSNELIEGENGDLMEVEREAGANTIGMVAFKITTRTPEYPRGRQFVVVANDITFKIGSFGPKEDEFFNKVTEYARKYGIPRIYLSANSGARIGIAEELVPLFQVAWNDENDPNKGFQYLYMSDEGLXQLKKDGKENSVVSEPIVEDGEKRNXIKAVIGAEDGLGVECLKGSGLIAGVTARAYKDIFTLTLVSCRSVGIGAYLVRLGKRAIQVEGQPIILTGALSINKVLGREVYSSNLQLGGTQIMYNNGVSHLTAQDDLAGVEKIMDWLSYVPAKRDMPVPILESEDKWDRDVDFKPTVQQPYDVRWMIEGRTAEDGDFEYGLFDKGSFQETLSGWAKGVVVGRARLGGIPMGVISVETRTVENLIPADPANPNSTETMIQEAAQVWHPNSAFKTAQAINDFNHGEQLPLMIMANWRGFSGGQKDMYNEILKYGAFIVDALVDYKQPIFVYIPPTCELRGGAWAVIDSTINADYMESYGDVEARGGVLEPEGMVGIKYRREKLLSTMARLDDTYRELKEQLAAKDLSAEAKEEISKKLTAREELLYPIYHKISVQFVDLHDRSARMAVKGVIKKELEWVNARRFFFWRLRRRLNEEYLSKRLTAQLPNASRLERIARIRSWYPASVEEDNDRQVATWIEESFQILEEKLKSLKLESFAQNLAKSLRTDHDNAISGLSEVLKLLSADDKEKLLKSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF46</accession>
    <name>A0A1E5RF46_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1879</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF46"/>
    <dbReference type="FunCoup" id="A0A1E5RF46">
        <property type="interactions" value="1119"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF46"/>
    <dbReference type="InParanoid" id="A0A1E5RF46"/>
    <dbReference type="OrthoDB" id="2449818at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.190.20:FF:000006">
        <property type="entry name" value="Ribosomal protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.790:FF:000002">
        <property type="entry name" value="Ribosomal protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.190.20:FF:000009">
        <property type="entry name" value="Ribosomal protein L10a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_uL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023673">
        <property type="entry name" value="Ribosomal_uL1_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01199">
        <property type="entry name" value="RIBOSOMAL_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="217" mass="24392" checksum="249B7A080E374780" modified="2017-01-18" version="1">MSKISSSQIREHVKELLKYSTETKQRNFLETVELQVGLKNYDPQRDKRFSGTLKLPNCPRPNMSICVFGDAFDVDRAKSIGADAMSVDDLKKLNKNKKLIKKLSKKYAAFVASDVLIKQVPRLLGPQLSKAGKFPTPVSHNDDLYAKVTDVRSTIKFQLKKVLCLAVAVGNVEMEEDILVNQILMSVNFLVSLLKKNWQNVGSLVIKSSMGPAFRLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF59</accession>
    <name>A0A1E5RF59_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Histone acetyltransferase type B subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1801</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF59"/>
    <dbReference type="FunCoup" id="A0A1E5RF59">
        <property type="interactions" value="1099"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF59"/>
    <dbReference type="InParanoid" id="A0A1E5RF59"/>
    <dbReference type="OrthoDB" id="427795at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050459">
        <property type="entry name" value="WD_repeat_RBAP46/RBAP48/MSI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22850">
        <property type="entry name" value="WD40 REPEAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="7">
        <location>
            <begin position="14"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="153"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="281"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ85531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="391" mass="44030" checksum="010533EB6A20A0BC" modified="2017-01-18" version="1">MSDVEEHQPETVEDEYELWKSNVPLMYDFLSETKMLWPTLTTEWLPSAADSLRQELIIGTHTSGGEQNYLKIAAIEMPIDVLSNKQSVSDEDQPHASKPKLKIAKKFEHELEVNRARYMPQDPDFIATINGDGKVFLYNRSVKNKKDALVSKFEIHKDNGYGLSFNPNAKGQLLSSSDDSTIALWNVESSLIHLYESHSDIVNDSKWHNFYSHYFASVSEDKTFKFHDTRTTAVVNTIKTAVPFNTLAFCKHSEHLVAAAGNDTLVYLYDTRNTGKPLHVMSGHADSVTSLDFASHQDGILCSSGEDRRVITWDITQFGAEQSADDAEDGSPELLMMHGGHRSIVTDFSINPNIPWLMASAEEENVLQIWKPSRKLTHPSAYTKFSIGDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFC5</accession>
    <name>A0A1E5RFC5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PAB1-binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2032</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFC5">
        <property type="interactions" value="782"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFC5"/>
    <dbReference type="InParanoid" id="A0A1E5RFC5"/>
    <dbReference type="OrthoDB" id="1937934at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="188"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="276"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="476"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="550" mass="60508" checksum="CD088148DFC9404A" modified="2017-01-18" version="1">MTKFKPTEKAGSKNQKMDADTFILTKETKKTGDAKKAFAIRPNHQDVARKGTLPHVFPETHLEEIETGRSTSRCSVLSNDTDEEYTKNKPEGLDTDYSNLHLSSLDGADKPSLLSTNGMASSLIKKRKNIETDEAELSLEAEIKRVALEDTTQFDSAQSYEKKYKLMKTDAVLGRKRSDVSIPDKFDKEFTMRLLCHVKEAALIIGPKGTFINNLKAATKTHVITSENIEGVQEXVITVKGAICDVAKCGSLIAQKLIFPHCDFSELSCVSESDFDKCGLKWLVPHELVGFVIGKGGNTLKLIEQENXAQILVSPHFLPASNERLVTVSSTTYLDATIIDIAKILVDSKKRISFKNNYYNPTSRSVHAQEMAQGQRYGLLQNSNHANKKSLSITGIDSAPPHLRPSENANQKNNYSFELSPFTLNAPGSLMPNSGLRNNIPTLPPSSVPLAGAPIPNTYSVFPFIKLAPYSSAPLGIIHQDCFILDVYVGKIIGKGGKNIKFVKKNSGAEILIDQNPHQGERKFTIIGTPMSTQIALAILNRKIESIIRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGW6</accession>
    <name>A0A1E5RGW6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">AP-3 complex subunit mu</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1422</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGW6">
        <property type="interactions" value="148"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGW6"/>
    <dbReference type="InParanoid" id="A0A1E5RGW6"/>
    <dbReference type="OrthoDB" id="870at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030117">
        <property type="term" value="C:membrane coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MHD" evidence="3">
        <location>
            <begin position="296"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ86106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="590" mass="66527" checksum="76CADE6A598AE8FB" modified="2017-01-18" version="1">MFDELNAIALYITPMKRLQNATGKTPVFQYCFEPKTYDTYFQNEIPKPVAQEIXSEXSIIXLHHGFALYSYTNEQDNLKYWLLCLKNKQAQKDKRAGATIDPVQNDFFAIKXIETLXKLNDVIKIYFESFSMDIKKTTSSISNAINSVAVVGHTRSLSTMRSGSSNNRLNAFGGGFGSSGNDGLVPSGAQSNASTGGTVGSDHVLNIRRIIKDCDRIALIIQVCNEMNGTDDFNRLVELVPFRKTFSDFINATTKSLSNVKMSTQQTDSLKIMRASQINREKRVVPWRSSSIKYINNEIFVDVIEETSMVINTLHRKSKHGRTNQYDKGSKKVIAYETIKGKVDFDSHLSHDSPRLQLKFSTKDPYTKSYHQCVNLDSNNHVSKHEPVTSGSLQFVPXDGKXTLMKYNIFKDXQQTAGGSGTSYSSTNPAQKNTISLDYENGLGIEQDQFEIKLVVGNNMMKIEEIKDLKVKVFFNQFSHTNDDLKVINSTHGYIEKRSKPNSDVSFYEWVFTSDELKKLIVGSMVSIRLYTLLKAFPSRLSMSYTIVGGKLQYNNLQVNSLEVINNAQSLKPFKGVKYTLIMKDCVVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RH06</accession>
    <name>A0A1E5RH06_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Spindle pole body assembly component MPS3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1128</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RH06">
        <property type="interactions" value="390"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RH06"/>
    <dbReference type="InParanoid" id="A0A1E5RH06"/>
    <dbReference type="OrthoDB" id="4065610at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034993">
        <property type="term" value="C:meiotic nuclear membrane microtubule tethering complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045119">
        <property type="entry name" value="SUN1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012919">
        <property type="entry name" value="SUN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12911:SF8">
        <property type="entry name" value="KLAROID PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12911">
        <property type="entry name" value="SAD1/UNC-84-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51469">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="SUN" evidence="7">
        <location>
            <begin position="538"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="145"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="775"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="8">
        <location>
            <position position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="798" mass="92422" checksum="5BAB887975C629B0" modified="2017-01-18" version="1">MESKDSSVRVSAAKSPSSTKAAQIATNRLFQNSRDGNNDGLGNRSNRGSSEVSSSAFTESLIFNQSANEALIDVLKKRGVPEEIYHKNVARMIRRKNLQKEEQFTNFEDEDMSVARDLLEQGGGVENAHTSQNFVSGTFANNCNSYSDEDKNQVRENGNDKTGHEGDVESEKKNEYSHFGKTGQDVIQWEHSDIKGLDEQLHDELGEYDFHDIDSLIDSNKDDEEYQEISSELDYSDRASEYESDDSDNIYRNGHFEGLYLSKDGRIVSAEEYMREKDQLKQYMVVTKRKVQWKRVISLLSIVFCFLLFSAHSVYQFQTVKYLDKQIHEIYHDRMELDKTVNVILQQVENKMKNLFHSNRFTNSNTTIVPPLNXTEIELSVYKQVESGFLNKLADFLPSEIPVIIKKNSKDDQNKILILPEFEQLFRNHVVNFPAKNTSKNALEFDETLQKTMNQYIKEVLLNEYQFVDKQFFXHELYKNINDIKTMFLHESEKFGDLQYKYNLQDEVFVRNLIQQEIFKEFERKTLYDRNGKFSRNTEQDCFNELDYATFSSGTRVINNLCSXTFKGLIDKGXYXNGIGPVDLLRDTELASLNDXLGNNNFYWFCDITKGDNQYCQWTARFHQPIYLHRIAYQHGRFKHNLSLMNSAPKTISLYVKIQDKTKRKQLTASYRKEMGAPIQLMYNDRSFIKVCNMSYDISNMQDMSQFFQLPAWFLKQKPLVKGISFVVENSYGNPYYIAXNKFVVNGFTPYDLELMALSASSSFSSNNGDFATSGFSNSNDEQSDSRYXSFGDDDEVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHJ2</accession>
    <name>A0A1E5RHJ2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Proteasome subunit alpha type-6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1019</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHJ2"/>
    <dbReference type="FunCoup" id="A0A1E5RHJ2">
        <property type="interactions" value="1135"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHJ2"/>
    <dbReference type="InParanoid" id="A0A1E5RHJ2"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000016">
        <property type="entry name" value="Proteasome subunit alpha type-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 7 9" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="8">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="233" mass="25752" checksum="675986181F82B155" modified="2017-01-18" version="1">MFRNNYDGDTVTFSPTGRLFQVEYALEAIKQGSATVGLRSKKYAVLVALKRNADELSSYQKKIIKCDEHMGLSLAGLAPDARVLSIYLRQQCNYSQLVYNRKLSVEKAGNLLSDKAQRNTQTYSGRPYGVGLLIIGHDNSGPHLLEFQPSGNVLELYGAAIGARSQGARTYLERVLEEYLEIDDPEQLIKHGVEALKNSLKDETLSTSNLSIAIVGEDKPFTVYDGEAVAPYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHM1</accession>
    <name>A0A1E5RHM1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">Ribosome assembly factor mrt4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1392</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Component of the ribosome assembly machinery. Nuclear paralog of the ribosomal protein P0, it binds pre-60S subunits at an early stage of assembly in the nucleolus, and is replaced by P0 in cytoplasmic pre-60S subunits and mature 80S ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Associates with the pre-60S ribosomal particle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
            <location evidence="7">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHM1"/>
    <dbReference type="FunCoup" id="A0A1E5RHM1">
        <property type="interactions" value="1114"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHM1"/>
    <dbReference type="InParanoid" id="A0A1E5RHM1"/>
    <dbReference type="OrthoDB" id="10262308at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05796">
        <property type="entry name" value="Ribosomal_P0_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1730:FF:000005">
        <property type="entry name" value="Ribosome assembly factor mrt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000003">
        <property type="entry name" value="Ribosome assembly factor mrt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033867">
        <property type="entry name" value="Mrt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051742">
        <property type="entry name" value="Ribosome_Assembly_uL10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841:SF1">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 MRTO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="8">
        <location>
            <begin position="135"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="238" mass="27324" checksum="3B0B20B4AD4AA5EE" modified="2017-01-18" version="1">MPRSKRSKLVTLAQTDKKGRQNKERIFDEVREALDTYKFCWVLHLDDVRTPVLQEIRQAWEGSKLIMGRRKVLTKALGEHVEQEYKENLSKLVKHCSGVTGLLFTNEDVDTVEEYFKSYTKSDYSRPKSKSPLAFTIPSGIVYSRGGQIPEEDDVPMVHSLEPTLRNKFKIPTKIKNGKIFLENEFLVCEEGQTLDVTQALILKQFGIASSTFRVKLAAYYNNESCEVIPVNINMDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHP4</accession>
    <name>A0A1E5RHP4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AWRI3579_g1394</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHP4">
        <property type="interactions" value="438"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHP4"/>
    <dbReference type="InParanoid" id="A0A1E5RHP4"/>
    <dbReference type="OrthoDB" id="3972588at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01093">
        <property type="entry name" value="CRIB_PAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06614">
        <property type="entry name" value="STKc_PAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000011">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000705">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.810.10:FF:000007">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033923">
        <property type="entry name" value="PAK_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF22">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SAMKA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRIB" evidence="15">
        <location>
            <begin position="522"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="1017"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="595"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="696"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="744"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="916"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="968"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="57"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="70"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="136"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="152"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="220"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="285"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="319"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="360"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="381"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="395"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="459"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="615"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="648"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="696"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="921"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="968"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="1046"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OEJ86421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1301" mass="142752" checksum="0DD778139A091066" modified="2017-01-18" version="1">MSEQRNSTHSEANVDGNEKTSTKETAFQQKNKDQLAVDYRTMDPGKPKNDMDNVTKTFFSSTNNSSSALSKEDAHDKQRQTSDINEKESQLRSSSKTDSRVLDGPVQFTRVSSVSLPSTSSFESSREDIPENYYDVQKEENKVFKADEDSGDLTNHSETPLLPKTTSNSVAGAAVTKSTPTRADNDNEEDQDKTARFSSVTTSNTSHTSNNGSSTGTPLLVNSQSSLKFTGHSPNLEHQQPENQNRQPLSQKEDTFQHCGPQKKPMNSSNGVVVEMDILKSQLSNDDEDEDEDEDEEDADLMPPSQRTPDRSTFKGQDFYQSPLTEILSSSESFNRPVNEEPKAQLDAIEDFKKSDKSKNTQNGCDSKNKQHNQSLYTKGAESKRVDDGQKSNTKETSVVGANSLPTQSTARVLSTSKPTKLNTTKPITEFNGRMNVTAKKNIKTEPINKTVPISQRNVSSSSLNSSGSTSTSSSNKKSKNVFSSFVRNMKRNSGGSNNSKRSSRGSGNQRRSYGSNGGLKISTPYNAKHVHHVGIDDKTGEFVGLPQEWERLLTSSGISKKEQQDHPQAVMDIVKFYQDVTDSNGDDKILKTFNKEPGMNLSSADNAAKPPKSSAKSHLPTTPSFNTSSEALRKRTKKKPDLEQRRSSNSVVSSSSSSFNITSSTNEDSALHSMRNISDSSVANDPLMSNKYTSVISSTSNNRSSNGTSGPGNTSGQGPLLSQQQPIFVDPPVLATENTQFIPSRAAPKPPANALSPKNXVSPLTQMSQSSSKMSLSSNISHHNAHLQQLPGQSDQQASEKTGIFRSNTMGKTHLTDILGISKQPNTQQQQQPSITKSVSAKVLPNIRSSAATNXHKFSSKLEKKSHLQPAVNIEAATEKLSKIKLPRSVTTPNLPSSSKEIVXDMHTDLNPAKAIASVPSPSEPLSQTPKTSSLRSPGKVSTPENNIMPPVPTAINVTPKLEMEQADSSKMREEAKRLNKEKQAERKKMEQRRKNKDLVDRLLDICSPGDPSTMFKNLVKIGQGASGGVFTAQEIGTRALVAIKQMNLEKQPKKEMIISEIIVMKGCKHPNIVNFIDSYLLHGDLWVIMEYMEGGCLTDVVTNCILTEGQIGAVCRETLKGLEFLHSNGVIHRDIKSDNVLLSMQGDIKLTDFGFCAQINETNLKRTTMVGTPYWMAPEVVSHKEYGPKVDIWSLGIMIIEMIEGEPPYLNETPLRALYLITTNGTPKLKDPDSITKLAADFISRCLTVDQERRATSSELLQHPYITTVAEKNDSLAPLVKLACMKKVQESIQEGDSHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHP7</accession>
    <name>A0A1E5RHP7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein LOT5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1245</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LOT5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHP7"/>
    <dbReference type="FunCoup" id="A0A1E5RHP7">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHP7"/>
    <dbReference type="InParanoid" id="A0A1E5RHP7"/>
    <dbReference type="OrthoDB" id="19714at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039924">
        <property type="entry name" value="ICln/Lot5/Saf5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03517">
        <property type="entry name" value="Voldacs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ86404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="291" mass="32532" checksum="F72FA5F343581BBD" modified="2017-01-18" version="1">MSNVIYTKPTIENVIPFELYKSTNPSLQFPNASSRFASISDTSLNPLLPILFGGGRNFTLTRMRNESFDSGSANPGGSDLPGSTSNLCSAPSCLVDLFVMDCGIVVWFDQLNMGIQIQYSDLVYHGIVTLPNDPHQRLLVNVDVTAGLEVIWEIFGPGIASTTTTTTNNTTTSRDNSNIDLLFTPMYYENDRFYNSDIENLFQFQWFGLNKGNSMIKNINDALDHCMETYYTTTDYQNQDLDDENDNGYDLDNEAEMQTMVDNTAEYDGISVGLQHYYYHNNTGMADDLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHQ3</accession>
    <name>A0A1E5RHQ3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g1258</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RHQ3"/>
    <dbReference type="InParanoid" id="A0A1E5RHQ3"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ86438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="298" mass="34108" checksum="187F294C80E484F0" modified="2017-01-18" version="1">MKIDFNVVIKVIARVTILFGLFGAIIPLLSFLSTKNLYQHVTESTKDEHDVEFPTLLKSPQVVYGNPAFTNFTTSNDSFVFAFPLNFLHSNIKYGLPEIELFFSLNKQHCYKEDLDIYTMDILESLKGPRFDDDFILRENLVSVQGNSIYGKLNLENSNIVRLWNFLFTVTPSYNGQVKATHIADGIQKLTIGHSNPGNVVLQNAPTYLDTEHSLYVLEIKIVINYPTTSYFRETCKPNLVIFDGSTLNVKKDFKQLNTVKKFMIQHRLISLLLLFTFHYMLLSVSIFFSSSISTLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHS9</accession>
    <name>A0A1E5RHS9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1441</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RHS9"/>
    <dbReference type="InParanoid" id="A0A1E5RHS9"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="34"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="235"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ86434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="380" mass="42249" checksum="0AF2E4539CE1EEFC" modified="2017-01-18" version="1">MTDTKVXVPEVNEATEILEVKETPETVKVERESGSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSDSSSSDSSSSESSSSESSSSDSMSPEDYSSESSSSDELDNIEGEDNEPLYVVVDEYPEGDYEEGDYDYSNGEGDYDDEEGDYDYSKGMDPTNLVKKAKNNDAVEEDDIYDYKDYVNEDDLYDYEDYGNEDYGNEDYGNEGAYEDYSGDDLISDSELLQAIRSDSDDNDLYDSYYESDXASSSPSEIYSDEDENEDEDEEEIYQSDSERDAEDDALVGELVKMDSDLANQFEELLYLNGKLDTYTYAMNKKFDQEIDILERSVSSMKRILLLILIVFAAFLVXQYSSVFGDLHWNINKGHLLSDLSSKYHTTKNVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHT4</accession>
    <name>A0A1E5RHT4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Class E vacuolar protein-sorting machinery protein HSE1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Class E vacuolar protein-sorting machinery protein hse1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g1388</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the STAM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RHT4">
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VHS" evidence="14">
        <location>
            <begin position="14"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="255"/>
            <end position="314"/>
        </location>
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        <location>
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        </location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="416"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="175"/>
            <end position="186"/>
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    </feature>
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            <end position="253"/>
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            <end position="459"/>
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            <end position="483"/>
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            <end position="504"/>
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            <end position="524"/>
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        <source>
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        </source>
    </evidence>
    <sequence length="524" mass="59876" checksum="C16881870A538A09" modified="2017-01-18" version="1">MVKRAKLSAAFRKATDPSLRTDNWGYVMDVVDLVKSGKGFGFGEHDEYDGNEXYDFYNVHTANNSENIMALISQNLMQENANVILRTLTLIIALSENCGSRIRVALSSKNFTTNVLYEKLISNSDVHITTRKETAKTVEKLSEIFSSDPSLKNMSDLYKKIQKKHPYLIESSKVVTSKNGPNVPQKTETHEGKKNADYDEEKMLEEALKLSLQEYEQTSKTPEATDTVVAHKRRSDVNSQNEQIQQADQRQQQPPAINKVRALYDLQSSEEDELSFKKGDIIRVIEPVYKDWWRGSLNGKIGIFPVNYVVAIPEKTEQEKLREQKEEKFILDKKQKVDSLQAVLAENKHNSPDTILQNEEITQLYGEVTPLRPGLTRMIGKYAYKKEELLRLNKVLNDSEKLYSSMMHQATSIYMNPPQQQQQQQQQQPQIPGAHPVPFSGYAQAGGNMQQQQSQPGHGIPAXHNAQRQFAPQDVYPNSSTPYPITPQQTQNLLQQHQPQQPQQYPFSRGDFGAQYQNYQQPYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RHU6</accession>
    <name>A0A1E5RHU6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1042</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ELP5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="UniPathway" id="UPA00988"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Elp5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Elp5"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15641">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15641:SF1">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Elong_Iki1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="315" mass="35663" checksum="C9C5956CAAC69DC8" modified="2017-01-18" version="1">MASSTHNPSVLLKRLLSLKDPSPLYLITDTIAHTAKNSILNEFAFQSVSDLDILYVSFETLNKPHYVSDFVRASQYFQSSQTLVAEVQARIKLLHAKNPNAIILCVFDNIGYIDSSEVAKIVQMLAEPRVSLCAIYHRDNLGHVESQPTGLKDGILPQNVYPSTYDLLKYIATNVLEVYPDVSSNKNIEDEEELESLVSRFSLPKGLNNLHSYELRLINRRKSGRSLKFDFIVNTETHEYVHKIAKDLSSEDASDPSFQGLTTFNLNTTTKQKHAKDQVDLPFLEAQSFNTGGAIVYEFEKDDDYDEEDPFEDPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI13</accession>
    <name>A0A1E5RI13_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Pre-mRNA-splicing factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1535</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI13"/>
    <dbReference type="FunCoup" id="A0A1E5RI13">
        <property type="interactions" value="192"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI13"/>
    <dbReference type="InParanoid" id="A0A1E5RI13"/>
    <dbReference type="OrthoDB" id="447637at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.790">
        <property type="entry name" value="Surp module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045146">
        <property type="entry name" value="SF3A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022030">
        <property type="entry name" value="SF3A1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000061">
        <property type="entry name" value="Surp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035967">
        <property type="entry name" value="SWAP/Surp_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15316">
        <property type="entry name" value="SPLICEOSOME ASSOCIATED PROTEIN 114/SWAP SPLICING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15316:SF1">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12230">
        <property type="entry name" value="PRP21_like_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01805">
        <property type="entry name" value="Surp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00648">
        <property type="entry name" value="SWAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109905">
        <property type="entry name" value="Surp module (SWAP domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50128">
        <property type="entry name" value="SURP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="SURP motif" evidence="7">
        <location>
            <begin position="28"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="SURP motif" evidence="7">
        <location>
            <begin position="120"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ86515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="394" mass="46016" checksum="07A14B8384E1FCEF" modified="2017-01-18" version="1">MMVALQELHDKTVPSSIASPELRSVKDSILKTALYVINNGKSFESKIITSEQNNKNFSFLHEGDIYHEYYKFLIDSYVQENSHTNSTDTNVFKNAPVGDPLDHLFLLYKNFHVSPKDLKVIKLTALACSLYDYHSTYENMKLRYAENPQFDFLEPEHSLYPVFANLVNQYTLVLSSEKDKGNTILTQDTNDPLWQQAFLERCYLKGAYNEFLEEKSVNDLQQLKVMKLRYNAIDWYNFKIMHKFDFAQPSDEKPKPALSFSDLKKNTLLTSQSELLSQYFDLKSQESIKETYRKRKSKKRNMVIKAAGESRMSEQKRRLDLTSNIVTNIHTSGMIQCPITAQMVKESDFDNHIRLLLQDPNYASEKRRFKEDNQLSNLTDHQVYENIKKITSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI23</accession>
    <name>A0A1E5RI23_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein disulfide-isomerase</fullName>
            <ecNumber evidence="4">5.3.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g1636</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 17">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI23"/>
    <dbReference type="FunCoup" id="A0A1E5RI23">
        <property type="interactions" value="780"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI23"/>
    <dbReference type="InParanoid" id="A0A1E5RI23"/>
    <dbReference type="OrthoDB" id="427280at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02995">
        <property type="entry name" value="PDI_a_PDI_a'_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02982">
        <property type="entry name" value="PDI_b'_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000139">
        <property type="entry name" value="Protein disulfide-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000017">
        <property type="entry name" value="Protein disulfide-isomerase A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005792">
        <property type="entry name" value="Prot_disulphide_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01130">
        <property type="entry name" value="ER_PDI_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929">
        <property type="entry name" value="PROTEIN DISULFIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929:SF132">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13848">
        <property type="entry name" value="Thioredoxin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10 11" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184756" description="protein disulfide-isomerase" evidence="13">
        <location>
            <begin position="24"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="14">
        <location>
            <begin position="12"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="14">
        <location>
            <begin position="352"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="524"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="527"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="11">
        <location>
            <begin position="62"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="11">
        <location>
            <begin position="416"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605792-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ86534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="543" mass="60908" checksum="46F8AE9200D2CEC2" modified="2017-01-18" version="1">MFMSKKFLLAGAAVLAAFNNVQAQQEAVAPENSDVVKLNSETFDSFIKTNSLILAEFFAPWCGHCKTLGPHYVEAASALKEFEIPLVQVDCTENQDLCMEQGIKGYPSLKVIRNGDALGASDYTGGRTATDIISYMKKQSLPAVSVFNNEKDLQAFMEKEQEVSPIVVNYDISEPNVNATFYYIADLLREEDYIFAALQNSSVSEDKSAISIQVPNQEPVSFDYSSLNVTNTTSVLNSLGDWLSVESKPYFGDVNAATYEPYMNAEIPLAYFFYTSDKERAKYTPFFEKLGKEYRGVMNFAGLDASKFGRHAENLNMKQQFPFFSIHDVVSDLKYGTAQLPDEKFVEGETKIKISEKAIAQFVEKFLDGKLEPIIKSEEIPTEQETNVTKIVGKNHDDIRYDEDRDVLIKYYAPWCGHCKRLVPIFENLADVYAADEEGQSKVVIGEIDATLNDVFDVDIQGFPTIVFYPAGSKNETPVVYSGPRTVESFISFISTNGNSGVDGFKILEAQRLAEEEQRLAEIEKLEAEDELEDDEEIEHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RI76</accession>
    <name>A0A1E5RI76_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative alpha-1,6-mannosyltransferase MNN11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1512</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RI76"/>
    <dbReference type="FunCoup" id="A0A1E5RI76">
        <property type="interactions" value="111"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RI76"/>
    <dbReference type="InParanoid" id="A0A1E5RI76"/>
    <dbReference type="OrthoDB" id="205108at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008630">
        <property type="entry name" value="Glyco_trans_34"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306">
        <property type="entry name" value="ALPHA-1,6-MANNOSYLTRANSFERASE MNN11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306:SF10">
        <property type="entry name" value="ALPHA-1,6-MANNOSYLTRANSFERASE MNN11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05637">
        <property type="entry name" value="Glyco_transf_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ86584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="422" mass="49403" checksum="42606DCE32A294DF" modified="2017-01-18" version="1">MEPKKSVSLKRRWKKFVHRWETQVTKMNKTQKFKYALPAGVFVLLILVFILTGAFPFKNTFTSRSLPPVHGLYQYDLKSSSPLIFPPIEHQPTLKKMGYKNLFLRASENSDYFVKTTNKPLTDKERKQMMNEENSKSTYAKTLFLDHGKTVYRKNTQPEVVIVTMIDYENFHLDTTVQIVQNRIDYAQKHHYGTYIRWAQEFIPFVEKQSLEESYEYMKPLMMRAAMNAFPKAKYFWFVDKNSLIMKLDLSLQSQLLDPKVLESNVLKGVQIDSNSVIKTHLSFDPKATIIIPQQQEISDVITLSLDFNSFIMANDMYSHSFLDYLSDPLIRNYKWNSFSQCVSHVLQWHPILLRKTMLINPKTIASVYDQVSNVFEKTSGKEKTTANQYEENNMLIEFKGCVETKSCGPLISKFYRQINRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIC1</accession>
    <name>A0A1E5RIC1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Importin subunit beta-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1007</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RIC1"/>
    <dbReference type="FunCoup" id="A0A1E5RIC1">
        <property type="interactions" value="1155"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RIC1"/>
    <dbReference type="InParanoid" id="A0A1E5RIC1"/>
    <dbReference type="OrthoDB" id="543373at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041653">
        <property type="entry name" value="Importin_rep_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040928">
        <property type="entry name" value="Importin_rep_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041389">
        <property type="entry name" value="Importin_rep_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057672">
        <property type="entry name" value="TPR_IPO4/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18808">
        <property type="entry name" value="Importin_rep_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18816">
        <property type="entry name" value="Importin_rep_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18829">
        <property type="entry name" value="Importin_rep_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25780">
        <property type="entry name" value="TPR_IPO5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="IPO4/5-like TPR repeats" evidence="9">
        <location>
            <begin position="118"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Importin subunit beta-1/Transportin-1-like TPR repeats" evidence="8">
        <location>
            <begin position="476"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1103" mass="123590" checksum="E6ED2CF78B284705" modified="2017-01-18" version="1">MSSSLLPQEVSQTLIGIVQAFASPDNNIRSQAESALNNTWITPENIDALLIFLCEQAAYSSDVTLSALSAVLFRKLAIRAPPNSAKSLIAKNITNISQTTLFSIRTILLQGFLDPNRENNIRHKLSDAIAECANEELPEWPELLESVFKIIADDTDAGSATDNSFKESCFRILSSAPHLINAVDFSMVIPLFEKGFTYDKNDDVKIAAVTAFVGYFKQLPKSHWSKLGYLLPSLLNSLPNFLSSGNDFALAAVFEPLIELVELAPKLFKEMFNEIVRFCDCVIKDRDLASNARTSALELLTVFSEYAPQMCKSSTVYGETLVMDCLMMMTEVSVDDEEALEWKISDDNDDDDQEEEYDHARQALDRVSLKLNGKYLVPFLFQYLEQMIVSPEWRQRFAALMALSSAVEGCRDVLMGKIGKILDITIPLINDPHPRVQYGACNALGQISTDFAPLVQRVAHQRIVPALISRLSSDCCERVQTHAAAALVNFSEEASQTIMEPYLDSLLSNLLVLLQSDKTYVQEQALTTIAFIANAAEKKFIKYYDTLMPLLINVLKTDTGAENRVLKGKCMECSTLIALAVGKQKFSTYSQELIDCFIRYQKDDEMKNDDPITSYLEHSWSRLCRLMREDFIPFLPIVLPPLIETAKASQDVTLIEEEEADNYQQYNEWDVVQIQGKHIAIHTSILDDKVSAMELIQIYASVLRNYFAGYVEEILLEIAIPSIDFYLHDGVRATGAKLITILLSSLISSTGVDTKVLELWSIAADKLVSGIMSEPMSEITQIYHSALADCIETINADCLTEQQVAAYTKGVSANLSDVYQRVNERRNNEDDYNEDVDNDLDDYADEDLLDEINKSLATIFKYKKEQYLPHFQTLLELISAYLHDTEQILVLFALVAIADMIQYCGEHTQPFKHLFINKVKEYLISPEPTIRQAASYIVGCCATSAPNLYADICNESLDVLLQVISIPDAKTEENLSATENASAAVAKILRTFNASLPNFNDNVQVWLKSFPIIQDEESAAFNYNFLCELIMKNHEAVVSKEALPVLLTCVVESLEKKVLRGDAAKSTINTMKQVLAQIPQDQIMTMFSGTSEEMMGNIQKWFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIE1</accession>
    <name>A0A1E5RIE1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Mitochondrial ATPase complex subunit ATP10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g1270</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RIE1">
        <property type="interactions" value="162"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RIE1"/>
    <dbReference type="InParanoid" id="A0A1E5RIE1"/>
    <dbReference type="OrthoDB" id="3971660at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007849">
        <property type="entry name" value="ATP10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28106">
        <property type="entry name" value="MITOCHONDRIAL ATPASE COMPLEX SUBUNIT ATP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28106:SF1">
        <property type="entry name" value="MITOCHONDRIAL ATPASE COMPLEX SUBUNIT ATP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05176">
        <property type="entry name" value="ATP-synt_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ86353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="321" mass="36870" checksum="7CB51897B5580E33" modified="2017-01-18" version="1">MLELALKAQTLQNQNSTRLTMSLKQFARALHSTPPQQFLFKKATKHLANTAPKEYTQFQLKTPCGLAEPPKHNIHYHEGNSMKEMFDDKKTAERTKELEKEFQQSGFHDLYAFEKTKGKLFFSPPQYWKKSYSKYFPHMVGQQLSTKKENVSVEDXMRGKVTVLKMCGNKVGEDMINTFFIKDTDNYLQDPEKILNQLNSDQSSVKDNGAXSNTGAIAQVPAQIVEINLIDNAMKSFLFKFFGNNLSKMVPEQRQDKYFICNRNQLPFGKREAINTNNVYSGYVFVIDPSLKIRWMACGSASSEEFETLWNSVQAIQKETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RIS3</accession>
    <name>A0A1E5RIS3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial</fullName>
            <shortName evidence="7">Glu-AdT subunit A</shortName>
            <ecNumber evidence="7">6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">HER2</name>
        <name evidence="9" type="ORF">AWRI3579_g1032</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Subunit of the heterotrimeric GatFAB amidotransferase (AdT) complex, composed of A, B and F subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.7" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RIS3"/>
    <dbReference type="FunCoup" id="A0A1E5RIS3">
        <property type="interactions" value="360"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RIS3"/>
    <dbReference type="InParanoid" id="A0A1E5RIS3"/>
    <dbReference type="OrthoDB" id="421993at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00120">
        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00132">
        <property type="entry name" value="gatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF7">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Amidase" evidence="8">
        <location>
            <begin position="28"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="7">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ86473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="466" mass="50523" checksum="144AA44DD43561E2" modified="2017-01-18" version="1">MATVFKDALFKRLENLPNLNKKFNMFNHLRKMNQIDLDVQSDGPLSGMLMGTKDNIVTKEMPTTCSSDILANYVSPYDATVVKLLQNAGVVNVGKTNMDEFGMGSGGVYSAHGPVLNPIYGKENRVAGGSSSGSAAAVACGAVDFALGTDTGGSVRLPAAYCSVTGFKPSYGRVSRYGVIAYAQSLDTVGILSKDVQTIEKVFEAVDKHDTKDPTSLSDHYRSLLKKESFPRYTYGLVEQFNQQCLSATSQNALLTVLEKLFDYKNTIKPVSIPLIENCLPAYYTLAPSEAVSNLARYDGIRYGHQDPSTDFKDGTLFAGTRSAFGSVVKNRLILGNYNLCSESFKNNYIKAQKIRVKLIDEFDAVFSTNNCLSSNKNNENGVDLLLSLTALDVAPTLSEFSTKSQQNPINAYINDVFTVPISMAGLPAISIPVPKTGLSLQIAGQYGDDYKVLAAAKQLEALLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RMX5</accession>
    <name>A0A1E5RMX5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Pre-rRNA-processing protein IPI3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g642</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Component of the RIX1 complex required for processing of ITS2 sequences from 35S pre-rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the RIX1 complex, composed of IPI1, RIX1/IPI2 and IPI3 in a 1:2:2 stoichiometry. The complex interacts (via RIX1) with MDN1 (via its hexameric AAA ATPase ring) and the pre-60S ribosome particles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the WD repeat IPI3/WDR18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RMX5"/>
    <dbReference type="FunCoup" id="A0A1E5RMX5">
        <property type="interactions" value="476"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RMX5"/>
    <dbReference type="InParanoid" id="A0A1E5RMX5"/>
    <dbReference type="OrthoDB" id="756370at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120330">
        <property type="term" value="C:rixosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045227">
        <property type="entry name" value="WDR18/Ipi3/RID3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18763:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18763">
        <property type="entry name" value="WD-REPEAT PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 6" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00026229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="563" mass="62555" checksum="64631D8F777249E8" modified="2017-01-18" version="1">MSTMDEQVLFGTSNATVLANLHNTTTSTVYSLRQSLPTCFQSLEASNDQNNLRVFTTLQNKALLHVYNIQKSSNALGGANGSGGMNAASGNASSSSSHVEVNISQRIPLVEPMEQFVILKGKFFVGTSGSGSGVGGLYIWDMESGNLLKFHQGLHYQKINGFVVSEDEKFLLTYSDDSRCCVWEIRDLFTAGSSSVGGFGESEKDKLKPVGVLKDHSLPITRVEIVADHIFTASLDMTVRCYGWIESGFSLMKTFVFSHRVYDFKLDAALRALYVVLETPSQQQQQQQQQQQQQQQTYGSSVVLQVPLLYNLSSGANESIASGTSDLKIVNLLNASQSQRMYTCTHIVDHEQGKAKLFQMNGQVECKKVYDGFPVSKVQLSLDNTILLVGLKKLGKILCMDIFSKQILKELHSNDRNDVIDIQISPIIKNNLGLVATGSNNNKNTNAFKYPIFNKNLNDENEFSDLNVMNMDTFENPHNDPNIVYSDFEQYLSEYVSPGHFLFQQVPTNPSLSLSTAASSMNKNVETILQDQEKDNQIQELQGTVAKLTKAYKDLRSMYSEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RMY1</accession>
    <name>A0A1E5RMY1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Vacuolar basic amino acid transporter 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g633</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RMY1"/>
    <dbReference type="InParanoid" id="A0A1E5RMY1"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF198">
        <property type="entry name" value="AZOLE RESISTANCE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="358"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="424"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="454"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="501"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="560"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="28"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="599"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="602"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ88250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="691" mass="77354" checksum="339D383239379229" modified="2017-01-18" version="1">MSALGEKFYNMFSSNGGFRQIDFRFCLIIVSLVMTMFIAALDIVIGISIINQISKDFNSYTNSGWVLSAYTLTSALFTLVSGRISLKIGLKMAIITSIVIFEIGSLISGVSNSMNMLIGSRVVAGIGAGGIQSLCYVSIPFLVGPTKIATLNAVFSATYGIAAFLGCIIGGTLIDRWRICYLINIPIGCVPLILLILVFPSSMELLNKKSNNVDELTFDMDIPGKQSPTNLITNTWNSFITTFFKLARNLINLNFYVDCFYELFAFFDLIEFSLISISFSLLLVGLTYGVQHGNADRAFNATYDVIIMLVFSGISFILAIVWDLKIYQLIVPSWYYKRYQRELCQPLFSIRLLTEWKLISLNMINFFISMIYFSVAYYSIQYFQLVFGESSSNATSHFTCFISAPISATIAGKLNSKFGLVKEVIILAVCLGMIGTGLLSRFDHQINSVSQAAPLVLVGVMYGGCVQTILLGLQIQTKDYGKDEKTENYKTEMTNFTSANSFFRILGSALAGVIANIVFDTTLKKKLNSNKSNLPVNLHKQSVNYIVIYRMENFDSRFSVLGQMIANSIVVVFWVTFAFQSVSFLLSLTLSNKRSMVSEVKSGPKDPFNKENHEMKEEQKALEEENGSFSEQHQSVGFNNNNLHQKQRTNVFDNDANSNSNNSFNTPQKTPWYFYIPVVGLGFRVYRWVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RN97</accession>
    <name>A0A1E5RN97_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g857</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SOS response-associated peptidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RN97"/>
    <dbReference type="FunCoup" id="A0A1E5RN97">
        <property type="interactions" value="632"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RN97"/>
    <dbReference type="OrthoDB" id="2111841at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106300">
        <property type="term" value="P:protein-DNA covalent cross-linking repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1680.10">
        <property type="entry name" value="SOS response associated peptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003738">
        <property type="entry name" value="SRAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036590">
        <property type="entry name" value="SRAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604:SF0">
        <property type="entry name" value="ABASIC SITE PROCESSING PROTEIN HMCES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604">
        <property type="entry name" value="DC12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02586">
        <property type="entry name" value="SRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143081">
        <property type="entry name" value="BB1717-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0190">Covalent protein-DNA linkage</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="230"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="230"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="246"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="274"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ88354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="305" mass="35545" checksum="519184743C13A61A" modified="2017-01-18" version="1">MCGRFALAYEFDELPQQIAKHKSLDPAPTISKDARDMKDDVKSKSYNIPPTKFCPVLNATDNQLYFMRWGLIPAFSDEPLKYTTFNARIESLKRSSIWKKPMEKHQYCAVPMSGYFEWRKTADKQPFYLKRKDNKVMFIAGLYERKREDDDSEHYSFTLITSSAPKGLEWLHARMPIILEPGTKKWNDWLQHGQITNAEFDPDLYEYYPVSREVGKVGNDNADLIVKKEETRLTPEKPATKRSRSKKEAAEGNHDVLKLFGKRSRKVEKSPQPVKEEHGNKRKGVKQEDSDEPHNKRIKTESNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNB7</accession>
    <name>A0A1E5RNB7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein AF-9 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g705</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNB7"/>
    <dbReference type="FunCoup" id="A0A1E5RNB7">
        <property type="interactions" value="741"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNB7"/>
    <dbReference type="InParanoid" id="A0A1E5RNB7"/>
    <dbReference type="OrthoDB" id="3970815at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1970">
        <property type="entry name" value="YEATS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038704">
        <property type="entry name" value="YEAST_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005033">
        <property type="entry name" value="YEATS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055129">
        <property type="entry name" value="YEATS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47573">
        <property type="entry name" value="PROTEIN AF-9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47573:SF1">
        <property type="entry name" value="PROTEIN AF-9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03366">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51037">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="YEATS" evidence="6">
        <location>
            <begin position="8"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ88374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="256" mass="29787" checksum="34111823A37CC68F" modified="2017-01-18" version="1">MSASSNRRIKYLSVAKPIVYGNSAQKLDENSKSVSIPQEHTHLWTVFVKSPTVNGDADLTKFIKKVIFKLHDTYKNPIRTIESPPFQVTESGWGEFEINIKIVFHDGSSEKIVSFYHHLRLHSYYLIPITDQKQLKEFPDQQFKTHKIEEKEGEKIHIDEDKNELSSSFFDEIVFNEPYIDFFQHCLLNTNILPKQKDSKQGVPPFSTELELEELHRMDESIAEIDTKITQYKERLMQLQKQQIDATASIVQGTQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNH5</accession>
    <name>A0A1E5RNH5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">P-type phospholipid transporter</fullName>
            <ecNumber evidence="4">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">AWRI3579_g946</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="4"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RNH5">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNH5"/>
    <dbReference type="InParanoid" id="A0A1E5RNH5"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140346">
        <property type="term" value="F:phosphatidylserine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006892">
        <property type="term" value="P:post-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000172">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF174">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE DNF3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="508"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1453"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1503"/>
            <end position="1522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1534"/>
            <end position="1552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1564"/>
            <end position="1588"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1608"/>
            <end position="1628"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="21">
        <location>
            <begin position="199"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="20">
        <location>
            <begin position="346"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="22">
        <location>
            <begin position="1390"/>
            <end position="1638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="696"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1064"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1708"/>
            <end position="1732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1773"/>
            <end position="1796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="756"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1076"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="15">
        <location>
            <position position="622"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="622"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="622"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="623"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="624"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="624"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="1128"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="1168"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="1248"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="1249"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="1250"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="1336"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="1342"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="1363"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
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        <location>
            <position position="1366"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="1367"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <position position="1367"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="OEJ88439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1833" mass="207575" checksum="09C1FB2E63F8BC41" modified="2017-01-18" version="1">MKRFGSKLQGRKFSRSSVIHEESPSLSSAGPLQGSPYRNAIAGTALAGEFEPYLVDMDLNSRTKSNVTTQAEATNSTLEVDNSQIELDNYSTREGPVARNETNELYQSNTEYKNDSFSQEQQQNLQEENVDSRAFLSPQRLNYFEKIRYFFSDLQKAVLNRSTLPPTANGRHIPLSLDKESIVYQDFHFDSEKHLLMDERKKYKKPYCDNVVTSSRYNIYTFFPRQLYAQFSKLANCYFMIVAILQMIPGWSTTGSYTTIIPLCIFIAISMAREAYDDYRRHNLDKDENLKKTRILFKSKQNNQFEKSKNKNPDVDLLPNIDHSLKTESENPTDYFTNVQLLKSRHDIEYVQTEWRNLRVGDFIYLKNDDWIPADMILICSDGNNNEVFVETIALDGETNLKTKNPHPEISKRMSTATGLVNTQGKVTVEDPNMDLYNFDGNIELLDSVSGTWAKYPVNSDNVLYRGSILRNTLNCIGMVVYSGEETKMRMNAIKNPRIKAPKLQKSINLIVFFMVFIVASMSLFSFLGYKVNENRFISNNRAWYLMESHVTTAESVMGFIIMYNTLIPLSLYVTMEIIKAMQSKLMEWDIDMYYKPANISCESRTATILEELGQVSYIFSDKTGTLTDNMMIFRKFSVCGSSWLHNLDELDSQENDENNQLGLTQTSTTSNSNVSVVSQSDQNFVQDILTRPNSLSAKSARANQAARMSGVSNGRKSMGRPSVEYRNYGSTNYTGRPSMASHIQRAEQIEEESISHTTTRSSDHSSPNLNPSSTNNSKGANGKGIKTTLELIRYVQRNPNTFFSRKIKFFLLSLALCHTCLPKRRAAPTNNSNGEEQNDETLDDDEKITIEYQASSPDELALIVAARDLGYVMVSKNSNVITIKTYPSGFDKPPVSEEFEVLNVIEFNSDRKRMSVLVRPRAEPEKVLLLCKGADNVILERLYNNKVASAKSAELNESTNERKKEQAELVLDQRKSLGGASVRRSGPFRPRVSVDIASRPSLSLQQVRNSLSKPYSARPSFNPHNRTSFDTGVNINSIDAFVENVNKNKQDVDEIAFQSRKSLHKQQREKYKNTALGSNSKNFDLTNKTDNMNSVATSLANATRSSPAKSSLPDTPSTQLGTFSCNKFSIDQTIESYIGGQELCENEEYVIERTLEALEEFSTEGLRTLLYSYKWIDQTEYEEWSEQYHEAKTSLVERAQKMQTVGELIEKNLVLLGATAIEDKLQDGVPEAIEKIRRAGIKMWMLTGDKRETAINIGYSCNLIHEYSTVVILSESDENILLKINTMSQELDLGDVAHSVVVIDGATLKTFESNPVLMGAFVDLCTKTDVVICCRASPSQKALMVSSIKETDKNMVTLAVGDGANDIAMIQSADIGVGIAGKEGLQASRSSDYSIGQFRFLLKLLLVHGRYNYSRTCKFVLSTFYKEAMFYATQMIYQRWTMFSGTSLYEPWSLSMFNTLFTSLPVLCIGMFQKDLKASTLLAVPELYTYGRLSQGFGPGVFFRWLTLSACTSVLITFLNLELFGKNVLVDNSIYPMGFINFTAAVFIINIKIQFLEMRNRSPIEFACVIISCGGWLVWCCFLPVIYSQELEYDVKYGFYHHFGKDYTFWATILLIMAIPLLVDMLFKVLKNSLWPTDSDIFAQLEQEDEIRKKLEMQAFSELEQGWTWNKDPSTFSRYTDKVVGTMIPNKVIDNVQNTSSAYLNESHSSSSLGIGDVDNSANRNVAEQQRKTRKNTILSFYNNTNPNFNQDSLFDSTKFEVLPSGRIIKKRSYTNDGGVSTGEGSGNDESNLSRESRKLKNAFSKTLRFKLQTENDEDIEAIIANRMKDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNY4</accession>
    <name>A0A1E5RNY4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Non-structural maintenance of chromosomes element 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g913</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the SMC5-SMC6 complex, that promotes sister chromatid alignment after DNA damage and facilitates double-stranded DNA breaks (DSBs) repair via homologous recombination between sister chromatids.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the SMC5-SMC6 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NSE4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNY4"/>
    <dbReference type="FunCoup" id="A0A1E5RNY4">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNY4"/>
    <dbReference type="InParanoid" id="A0A1E5RNY4"/>
    <dbReference type="OrthoDB" id="361242at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027786">
        <property type="entry name" value="Nse4/EID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014854">
        <property type="entry name" value="Nse4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029225">
        <property type="entry name" value="Nse4_Nse3-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16140">
        <property type="entry name" value="NON-STRUCTURAL MAINTENANCE OF CHROMOSOMES ELEMENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16140:SF0">
        <property type="entry name" value="NON-STRUCTURAL MAINTENANCE OF CHROMOSOMES ELEMENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15412">
        <property type="entry name" value="Nse4-Nse3_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08743">
        <property type="entry name" value="Nse4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4 7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nse4/EID protein Nse3/MAGE-binding" evidence="10">
        <location>
            <begin position="103"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Non-structural maintenance of chromosome element 4 C-terminal" evidence="9">
        <location>
            <begin position="301"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="154"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF15412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ88578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="398" mass="45928" checksum="6848D759937EAD45" modified="2017-01-18" version="1">MDKRTRGQRDSSQHLKKKLKKSGSQAYGLNKEGDHEGDRLEYQILQGYRTMDSEMVEQRVQLDREADINIAIQKLKDADKLFNQAGALMKDPEKTSNTILAYDSRALLNISELAQTSVRSLKLDEAKQNINSVDVLNGMKKWMLEEYLEETGLDQNLVDSDEENENINNEGNEQEVDENESLSQADIDKRLMTRRLKERSKLAKYEPFKQFEQFNWLKLGTLYRNISRKPNVVDHLLGPLQVEKKARAAPKPRVRDDPLGKEVTAKNMSNEDMNAKGAATTEEFVRACFKILKKKKGYESINLFEFIIDPNSFSKSLENLFYTSFLIRDGKLIMEEDADGYPQIRIMPPVPKDPEARALELQKRNEKPLSHLIFQLDIPTYEKLKQEFNITESFIGNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RNY6</accession>
    <name>A0A1E5RNY6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Guanine deaminase</fullName>
            <shortName evidence="8">Guanase</shortName>
            <ecNumber evidence="8">3.5.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Guanine aminohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g592</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>guanine + H2O + H(+) = xanthine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:14665"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:17712"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Purine metabolism; guanine degradation; xanthine from guanine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the metallo-dependent hydrolases superfamily. ATZ/TRZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.3" evidence="8"/>
    <dbReference type="EMBL" id="LPNM01000005">
        <property type="protein sequence ID" value="OEJ88592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RNY6"/>
    <dbReference type="FunCoup" id="A0A1E5RNY6">
        <property type="interactions" value="147"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RNY6"/>
    <dbReference type="InParanoid" id="A0A1E5RNY6"/>
    <dbReference type="OrthoDB" id="194468at2759"/>
    <dbReference type="UniPathway" id="UPA00603">
        <property type="reaction ID" value="UER00660"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008892">
        <property type="term" value="F:guanine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006147">
        <property type="term" value="P:guanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000022">
        <property type="entry name" value="Guanine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014311">
        <property type="entry name" value="Guanine_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051607">
        <property type="entry name" value="Metallo-dep_hydrolases"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02967">
        <property type="entry name" value="guan_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11271">
        <property type="entry name" value="GUANINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11271:SF6">
        <property type="entry name" value="GUANINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="9">
        <location>
            <begin position="95"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Amidohydrolase-related" evidence="9">
        <location>
            <begin position="402"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ88592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="520" mass="59566" checksum="7F318C1B1AD28FBE" modified="2017-01-18" version="1">MKRQTPEFIVFAGTFVDTPKLNELRIREKCCVGVDMRKAIKGSIVFIHENSCNFDNPIQDAMEYDGNLQEENIKVIQNKDIDQSNNENNNTKFFFPGFVDTHCHASQYPNAGIFGKTTLLDWLNKYTFPLEKSLQDLNIANKVYTKVIQKTLSHGTTTIAYYATIDLKSSILLAELCSQYGQRAYIGKVCMDRNSPDYYTETVDDCMESMENFLKYLSKVLNDGKIKPIVTPRFAPSCSDELMQKLGELSLNGNSNDTDNQQFEKIHVQTHLSENKEEIEWVHRLFPNIQSYTHVYDHFNLLHEKTVLAHCIHLSEEEANLIKLRKSGISHCPISNSSITSGECKVRWLLDKKIKVSLGTDLSGGYSPSILDTARQALLVSRHVAMKYDSLNHSMENECSKKGLEKGLKKYSEKYSKEHYKLSSKEVLYLATMGGAQCLDMENEIGSFEVGKQFDCQLIDLNCENSNVDVFNWQFVKSEEIDKEKELEKFNDLIDKWLFNGDDRNTTRVWIQGKQVISKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVG2</accession>
    <name>A0A1E5RVG2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sterol-4-alpha-carboxylate 3-dehydrogenase ERG26, decarboxylating</fullName>
            <ecNumber evidence="13">1.1.1.170</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18 19">C-3 Sterol dehydrogenase ERG26</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16 17">C-4 decarboxylase ERG26</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Sterol-4-alpha-carboxylate 3-dehydrogenase erg26, decarboxylating</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">AWRI3579_g547</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>4beta-methylzymosterol-4alpha-carboxylate + NADP(+) = 3-dehydro-4-methylzymosterol + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:33447"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:50593"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:64925"/>
            <dbReference type="EC" id="1.1.1.170"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:33448"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="12">Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 4/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterotetramer of ERG25, ERG26, ERG27 and ERG28. ERG28 acts as a scaffold to tether ERG27 and other 4,4-demethylation-related enzymes, forming a demethylation enzyme complex, in the endoplasmic reticulum.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 3-beta-HSD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.170" evidence="13"/>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVG2"/>
    <dbReference type="FunCoup" id="A0A1E5RVG2">
        <property type="interactions" value="414"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVG2"/>
    <dbReference type="InParanoid" id="A0A1E5RVG2"/>
    <dbReference type="OrthoDB" id="10058185at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000252">
        <property type="term" value="F:3-beta-hydroxysteroid dehydrogenase [NAD(P)+]/C4-decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09813">
        <property type="entry name" value="3b-HSD-NSDHL-like_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000346">
        <property type="entry name" value="C-3 sterol dehydrogenase/C-4 decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002225">
        <property type="entry name" value="3Beta_OHSteriod_DH/Estase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050177">
        <property type="entry name" value="Lipid_A_modif_metabolic_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245">
        <property type="entry name" value="BIFUNCTIONAL POLYMYXIN RESISTANCE PROTEIN ARNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245:SF51">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE_REDUCTASE FAMILY 42E, MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01073">
        <property type="entry name" value="3Beta_HSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0752">Steroid biosynthesis</keyword>
    <feature type="domain" description="3-beta hydroxysteroid dehydrogenase/isomerase" evidence="20">
        <location>
            <begin position="6"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00066634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00067470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00067985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00081267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00081452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00082106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OEJ90911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="346" mass="38025" checksum="57C133EC71ADC038" modified="2017-01-18" version="1">MESVLLIGGSGFLGLHLIQQFYEVSPRPKISVFDVRPLPEKLSKQFSFDPKEIGFFKGDLTSSADVSQAIKESGANVVVHSASPMHGNAQEVYEKVNVEGTRNVLDVAKSCGVKAFVYTSSAGVIFNGQDIHNADETWPIPEIPMDGYNETKAIAEEMVLKANDSANEFLTIALRPAGIFGPGDRQLVPGLRSVAKLGQSKFQLGDNNNLFDWTYAGNVADAHVLAAKKLMEAGDSASVAGETFFITNDTPTYFWALARTVWKADGHVDKKNIVLSKPVAIFAGYLSEFFSKILKKEPGLTPFRVKIVCAYRYHNIDKAKKLLGYSPKVDIEEGIRRTLLWMDEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVG4</accession>
    <name>A0A1E5RVG4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g533</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVG4"/>
    <dbReference type="InParanoid" id="A0A1E5RVG4"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="258" mass="28999" checksum="90EDEFA8B77C0F23" modified="2017-01-18" version="1">MSLNPAFPDYCIFCDQLIQQNKTPTTSLTCATAMASVGLNSKQKTLYCSTECFHRDQMLKNTNHDEKTLTKQSCKHDPSNCISALCNGSNNTSNTNEDNIGNINEDNSENNNENGNYNNLQMLSPISYNGDFVNNNNSSNTRDCFDLQDDFFQLDQDYNASHYSAVPSATVASTDYGNSGDSESREMGSHSHNTQFHVPSPSLQFDNTPSISKYVSLFDQSHQIFCKGALNQKRNYKQELNDGICENNYKIWLNENHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVH1</accession>
    <name>A0A1E5RVH1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Endoplasmic reticulum membrane protein 65</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g463</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAPT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVH1">
        <property type="interactions" value="274"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RVH1"/>
    <dbReference type="OrthoDB" id="5376140at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008010">
        <property type="entry name" value="Tatp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13317">
        <property type="entry name" value="TRANSMEMBRANE ANTERIOR POSTERIOR TRANSFORMATION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13317:SF4">
        <property type="entry name" value="TRANSMEMBRANE ANTERIOR POSTERIOR TRANSFORMATION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05346">
        <property type="entry name" value="DUF747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="188"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="285"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="365"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="545"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="579"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="51"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="124"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="124"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ90826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="681" mass="77273" checksum="3BF65E8A4E2F3D01" modified="2017-01-18" version="1">MSEKQSTPASLRKLKNFVLNELNHPETSKSNRTRSESVIVHEVXQXQAANDTFISPLNESPIATKKSKXSTQEKTLTSVRSKNSGKEDDTTKXQVAKHDFKVQGLGIHSVDDSALKTPVAQKVFMSNQPNTGKADTPSDRPKLMIQPSLPHNEPRAVSSRSGIPNISNDHELELLINMVKLPVYFEKLMAFSLLGCFNLFLYYFTMIPLRFVYLNYEFCRIIGINFYNRLFQNGFPPVVLAAEAAQGGTSAGRRHSRAREDAQKLHAFKSTKEIVSFLQVFRMDFILLVEMVITCVILLLSLDTSKAYHKIKGQNAIKLYALFGVLEMADKMLSTIGQDFLLAIYRRVSDSTLLLNNKTNIPSRYFLLLIIARWFYLTIHSTILIYQTIALNVAVNSYSNTLGTLLLSMQFGELKSSVFKKFDKEGLFQLAIADVAERFQLVSMLAIIALRNLVANKKSFTMSSSFPQSWNLNLIXFNQYTTGIIGVLCGPMVKVIGSEFIVDWLKHGYIGRFNRFKPEIYDKVFRKILVQDSVNHTNFKLQARLGVPIQAQVVTFIVLVLPKFKRMFYQMSTVKRTTVLFGVWLFLLTVKLATQLIIKKWCYHIAKKHETQTATASDKYYVPGELSGSQGLVDEPMRKIIHNDSANMPASAAXFRLKKDAKDPTSLEKVERYKMSSKKIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVI2</accession>
    <name>A0A1E5RVI2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flocculation protein FLO5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g516</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVI2"/>
    <dbReference type="InParanoid" id="A0A1E5RVI2"/>
    <dbReference type="OrthoDB" id="4036630at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000128">
        <property type="term" value="P:flocculation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001389">
        <property type="entry name" value="Flocculin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00624">
        <property type="entry name" value="Flocculin"/>
        <property type="match status" value="16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184897" evidence="1">
        <location>
            <begin position="23"/>
            <end position="1443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1443" mass="157219" checksum="43B29E0DCB98C431" modified="2017-01-18" version="1">MFLFKPQTLWVLLLWLNNFAKAARHGESTTSTVSTALQTSTIFVLTITTTGTCPSCSITSSLASKLVCFFTSFNSYNGKATVTYSMTSNVVANVTSTTFFVQTPIPVEDLTNLSSYTGTVPTTYSTDFFTYSDNTDATVSVIYYVQTPTSHTTRFTTTSWTGSSVTTYFTLYSTFIGEDSTQTIETIYYIETPIARVVSTSLSWYDGSSLLTYATSVSTITGTDDLETISTLYYVETPLAHTLSSTTTFWTGLVTFTFSTQYSTYVGSDSTKTVSTLLYVETPISRYISTYYSYWDGTTDSTYSTEVDTITIDGSPVISTVFYVATAEAHGTIERTGSWTGTVTQTYSTSFSTFSGDNGRATVDTYYFVETPITRVTETSYSFWSGSVNSTYSTEVTTNYVFNSSALISTVYYVATPEEQRSFTSTSHWSGLNTATYSTSYSTYIGVDGSATADTVYYVETPVSRYVDTSSNSFWNGTTDSVYSTSVFTATDSSNGSPFISTVYYVATPEAHGAYTKTTYWTGLNTATHDVSYTTYAGSDGHETVGTYYFVETPISRYISTSYSYWDGTTDSTYSTSVFTVTNGSSPFISTVYFVATPEAHATFTQTSYWTGMSTSTYGTSFSTFAGVDGYETVNTVYYIETPVSRSSSTYYSYWDGTTDSTYSTEVDTITIDGSPVISTVFYVATAEAHATFTQTSYWTGLSTSTYGTSFSTFAGVDGHGTVNTVYYIETPVSRSSSTTTVWIDGDGTSTLSTSVMTSFISSSPLLNSIFYVIVPARNVQEYITDYWNGRFTNTFSTMYSTYYGLDQQQTIKTLYHVEIPTSETMVNVTGYSGTITSTFSTAYSTQIGLQGNSTVVTIYYVETPTCFKTPARSSLTFFTSESVYQQSNNLTSSHSYIPKSFKFISTWSTFETAFNESKTSTYATNVYTLTGLDGFITLSTIFYVWTPISKSSSFKTKLPSTYFPIPSGTSTETLKSKAPTSTFCSSISTHSYSVDTIVSTSITTSGSMTSTYSTQIGTFLGANGMATVRTLLYIVFPSSITXTXQHTDPISSTETITNLNPSMNISTDPGTATTAVQFTTSYFVNTTFITLSLSSASLKSSYLTSALYHNTTSGFMSLSETNSPVVTVSYITSSTMIPVPTTGACTTLASNLGSLTNVIPKTSTTIGELTTTQATLDFSYLYSKSKVPIFPTQETLTVSFSSLSKSFLPPTSSSFHFFHTERTSEMYGTTNASSTNMDYSKIGSGFKASSPLLTPSLSTQITKNLVPTSDLSNSQYSQLPLYSKVSEDSSTKRVMTSSSKQESLQTTSSFLLASEVVSKSLGTYVSTGMLPPLSDTDLAFSSTSLNGFLKNSSSVCSFKSDSLSTFFPSETEVYLQENSSFSPLLTSSSFLTSLRPNPTNTRPIASPTLAKENGSXPALSFLKHFFVKFVVLTSCTVLFLFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVJ3</accession>
    <name>A0A1E5RVJ3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein BMH2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g406</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the 14-3-3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVJ3"/>
    <dbReference type="FunCoup" id="A0A1E5RVJ3">
        <property type="interactions" value="1531"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVJ3"/>
    <dbReference type="InParanoid" id="A0A1E5RVJ3"/>
    <dbReference type="OrthoDB" id="10260625at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023409">
        <property type="entry name" value="14-3-3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000868">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00796">
        <property type="entry name" value="1433_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00797">
        <property type="entry name" value="1433_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="5">
        <location>
            <begin position="6"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="73"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="101"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="2">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="2">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000868-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ90798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="287" mass="32401" checksum="82E2C398F0C2BA28" modified="2017-01-18" version="1">MSTPTRESSVYLAKLAEQAERYEEMVENMKNVASSGEELSVEERNLLSVAYKNVIGARRASWRIVSSIEQKEEQKQQQQQQQQQQNTTAPAQQPEQSTAEGENKEASNTETKPEQSSAVSTQVSLIKNYRSKIENELTKICYDILEVLDAHLIPSASSGESKVFYYKMKGDYHRYLAEFSNGDLRETATNESLKAYTNASEIATTELPPTHPIRLGLALNFSVFYYEIQNSPEKACHLAKQAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDIIDDGADEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVK0</accession>
    <name>A0A1E5RVK0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">Man(5)GlcNAc(2)-PP-dolichol translocation protein RFT1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g483</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Intramembrane glycolipid transporter that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. RFT1 could mediate the translocation of the cytosolically oriented intermediate DolPP-GlcNAc2Man5, produced by ALG11, into the ER lumen where dolichol-linked oligosaccharides assembly continues. However, the intramembrane lipid transporter activity could not be confirmed in vitro.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the RFT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RVK0"/>
    <dbReference type="FunCoup" id="A0A1E5RVK0">
        <property type="interactions" value="695"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVK0"/>
    <dbReference type="InParanoid" id="A0A1E5RVK0"/>
    <dbReference type="OrthoDB" id="9979195at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034203">
        <property type="term" value="P:glycolipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007594">
        <property type="entry name" value="RFT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13117">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MULTISPAN TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13117:SF5">
        <property type="entry name" value="PROTEIN RFT1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04506">
        <property type="entry name" value="Rft-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="241"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="386"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="422"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="455"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="483"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="524"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="552"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ90970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="586" mass="68343" checksum="738B9800A5FDF801" modified="2017-01-18" version="1">MSTESILSKSTKGVTFLMFTQFFTKILSFLLNSMLIRYLSPKIFGITAFLGFLQSTGLFFSREAIRLSTLRIQKSYNQQQQQQQQQGDVEEKTEEILGTEIPEDVKGLNEKSNADDNDDDEYDQNVLQSIINFSYLPILLGLPLSLVLFVWQYTKLNGYFTELPFFGFSIIVLWLSIIIELLSEPFFNIHQYLLNYKIRSQFEGSAVTVSCFVNFLIVYLHETRYNGQDGLELHNQFKEEGIAIFAFACGKFFYSLTLLLLYYRDYMRNFAPMKKTQLKITKIKSVHRTQSFYFQNEILQHFKKVYFQMCFKHLLTEGDKFIINALCTVEEQGIYSLLSNYGSLVTRLLFAPIEEALRLFLTRLLSKKTNANLKLSVEVLINLTKFYLYLTIMIIIFGPINSSYLLRYLIGSKWSSTSVLETIRIYCFYLPFLSLNGIFEAFFQSVANGDQILKHSYMMMCLSGVFLLNCYILIAKMKLSLEGLIYSNIINMILRILYCVVFINDFYKRLFHGKSSIIVNFHNLRVACFLSLLVWVLDWFFLGGYTKNLKELAINFLLALFLLSVMIYSERRVIYGFIRSKTDKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RVU7</accession>
    <name>A0A1E5RVU7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ERF1 methyltransferase catalytic subunit MTQ2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g336</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal PrmC-related family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RVU7">
        <property type="interactions" value="372"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RVU7"/>
    <dbReference type="InParanoid" id="A0A1E5RVU7"/>
    <dbReference type="OrthoDB" id="406152at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035657">
        <property type="term" value="C:eRF1 methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052190">
        <property type="entry name" value="Euk-Arch_PrmC-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875:SF1">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ90868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="296" mass="33723" checksum="CC269F9E50CC8B82" modified="2017-01-18" version="1">MSFCQPKGSQNSNSRIAITIDQHRNKADFFLKDMLPTPYVQCKEYDKVYEPAEDSFLLLDSLETEIPFLQKQFKNEQKPNYVPFVVELGCGSGILSTFMLQNSIPSLNSLYFPIDVNPHALEVTKNTIDYNLSMEEKSANGNLGNSTTKPHQSSAVPHIYSTLQMDLFFGFRPKTIDVLVFNPPYVPAEYVPKIPDSKQVFKDQNTQREADLIETNDDVWLDLALLGGPQGMDITVXVLENMDKMLTCDGVAYILFCARNKPEQVAQEWTNKGWKVELVEKRKAGWEVLSVYKFTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RWA0</accession>
    <name>A0A1E5RWA0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein GIS3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g374</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000003">
        <property type="protein sequence ID" value="OEJ90983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RWA0">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RWA0"/>
    <dbReference type="OrthoDB" id="4088353at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ90983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="533" mass="59829" checksum="2B03B2BC0CFF3045" modified="2017-01-18" version="1">MGVKIDYFIMNQREQIFNDIMLSKSNESSIDSTTRYSRKPSMVEHSNVQQIKTGHAAINNMYSHAATRRRRRRSSMEPMHKRRASSVSCVGNGSAPGTLSITPSLSSILNQRKSQKTNSESKKKTNYHYYHHVKRHTDTCPIKKSQQDQPHPLNDTITPRYCGLRDFKASNVTEAQIQTDQPQVEIFNDLQDLDLYTEMTLNDVVQFYSPVQTYTNTETEXLQQETCTSLSSVPNHTDSIEDDQSEKHIPCIELDNIPTQKPLEQPILSSSTPQTGKLGQLHNITYPKQQRHQSLSPHFMLLYSMEQNMKKTQLLPELDVDEVLLAKLSVQDIWNLEIPKPQNPSAATIEGCKSIADANTSSPNISDCSTAKASASNEGSSKCAWNEEDNIKLALITRKKLWCDMIQNSRVDTFGGENLPWNKKFVYNAVGSSASDKEGLTTNDCTIDGGKRASQKKVQSLVRLNSRKAPWMHVTDSSFSSSTPTPAANTTSQKVAEKLSKASTRQMIKPCGLLDSGRIQYVVKGWCDSRFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZ91</accession>
    <name>A0A1E5RZ91_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g26</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZ91"/>
    <dbReference type="FunCoup" id="A0A1E5RZ91">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RZ91"/>
    <dbReference type="OrthoDB" id="4068368at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="496"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="610"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="501"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="616"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="633"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ92320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="672" mass="75354" checksum="4C654214790320DB" modified="2017-01-18" version="1">MSIFDQKNNLLAAIKPAHQTKENTLMLSHTCLIAVAGINCTSCLALAGTSIALMKTITWMNKPSLIIITIASLLNATTEMLNVLLKRDKSIPKILKLCSVVFSVVVFASGVAYCDQLFKNIQSTDRTHLIIGQFSMLLLVLLTSKPKLEEIFESDIAHGQKDDLEQQIEPSSLNMHKKIVLKASDQTLRPEFEWFSYSQMDNYIKKHNSEAASNSDSSKLTKFSCWMKKAREFSKQEKTYTQNEKSGHAKYVTRLSSIPDMSFFSNLDQSNTHKQHGISLANLRSKEKTPEGLQQLEAEHHTLERISSVLLPPCLEAGASKHTDAASNIDEISSIPKETFTQQPSFKAHEHVKTVKVSSNPFFNDDSFSFPYPGTDDVIKEQKLMANSQKHVVPTQNYNTDTVEDLDRYLNSRKNSVYPDMEDTNIFQEKDFFNSTFNITSPKTLPPLKTSNLGLHGEFAHRYSKSQQMHSPSKSTTSAGSGLLANMFLQSSPTTSFKAKASSRRNSLKHKPSLSASSFATVRQVPFDNSKSSMSSPTRSTKGARLVNKLSLSNISLHSDTNTNTFSHNEHKRGKSIADFPYLSSLHHSHSHSPKKSMHSLRDRRMSNGSTIHLSFDMKDHIPTIKERDSQKRVQSGESAESGQTVESLDYPLALVSEYDKEKWRIMSEIDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZA1</accession>
    <name>A0A1E5RZA1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pre-tRNA-processing protein PTA1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g221</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZA1">
        <property type="interactions" value="237"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZA1"/>
    <dbReference type="InParanoid" id="A0A1E5RZA1"/>
    <dbReference type="OrthoDB" id="3970859at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032460">
        <property type="entry name" value="Symplekin/Pta1_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11935">
        <property type="entry name" value="SYMPK_PTA1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Symplekin/Pta1 N-terminal" evidence="2">
        <location>
            <begin position="100"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="196"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ92330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="752" mass="85917" checksum="4E0F6CE303AA20AA" modified="2017-01-18" version="1">MSVEQLKQAKQLALTSKNPEELLPQVLDTTFQLVDVPRSGPPPSFGLLNFAVDLLQDTLQHSSIPKNEHQFIALKHIGKLDQIFKCGFYYYKELELSMINIVSIMALVYEQVFNLVATTSNQELWDLMQSLSTQFTKLWDILTGKGLKYINEFKDDCFLTFNQITMKYRHTRMKIMLLKFFSKXVIIHTLGDGGASNSNDDPNTNSSGSNNNNSNNSNSSSSSSSSSTVSSTSSKNTVGQVSVSNVSASHPVIKNKAKIDADAKKLLDKLLNYLVDEEIMMCTQVFQCLLTNLVFVMQKRPQSSXRIFQAVLRFNLDKKFQFAKEDTLQYRLSKRFMEREYKNFVNFCYKTQLLNKNMNSLMSKLTKIASTLQMIGDDTKQKGILEYDENXVKNIIPLEVLQRYENPKPVVNLVRLRNNALKRSFFEMTDTVDEGNDSKENYVKDLYSTGKKSFDLSKISKNNLIKLSINTLMDGYKTQPLVTKLLETVLKYTQMSNDVSDFKLQKTDSSVSPTPMDAVMLQEPRQEGPADQLDDTSLLSLVSDISMSKEESLQHFEFLVNNILSSEGSLDGQTFNGNGENNDVSLINKKAAPAVNILIRLACKGIPSENMEYSNIIREKLYEYVIQDFSNRMDYVITWMNEEWYFEADPLKPVYNEWCNKVFNGIVPFVDNSHRRQFIRLVSELPSMNEQHINNFKSLCLDPLRNQLGFVTLKFMIMFRPPVVALIKSFLTNLKSEHADLQDQCNNLLSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZK7</accession>
    <name>A0A1E5RZK7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Zinc-responsive transcriptional regulator ZAP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g35</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZK7">
        <property type="interactions" value="358"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZK7"/>
    <dbReference type="InParanoid" id="A0A1E5RZK7"/>
    <dbReference type="OrthoDB" id="387657at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050329">
        <property type="entry name" value="GLI_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048420">
        <property type="entry name" value="Zap1-like_Znf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040792">
        <property type="entry name" value="Zap1_Znf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818:SF139">
        <property type="entry name" value="PAIR-RULE PROTEIN ODD-PAIRED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818">
        <property type="entry name" value="ZINC FINGER PROTEIN ZIC AND GLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21816">
        <property type="entry name" value="Zap1_zf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18217">
        <property type="entry name" value="Zap1_zf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="689"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="898"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="931"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="961"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="1001"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="1027"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="1053"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="48"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="112"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="348"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="465"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="352"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="479"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="8">
        <location>
            <position position="7"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ92314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1078" mass="120997" checksum="E559E02E136715A0" modified="2017-01-18" version="1">MSTEIDDAVRSVKREGTSNEDKIAKTITSDILKNEAVIDTTDENITTTTTTTSTTTTTTNANKNANTAEHPQKPKRKRESFVHGHIHNYNQLTYIHGHVHNSRKKPVDENVLQNGNLLDGGLTHGSADQNDYHSLPMEKIGSKEPXGNATGSVDINSSCLEYDDCQHFEFINYHNMDMFHAGAANGADNVLHELTDTTEKRPLLDGEQQQQQQQSGLSNKKIKTNSVNCKDTDCSPKIVEICCDNDHNPAMKAPPGTGSNFKNPHDIPTGGTSGSTTTATPLNDFVNCCENDTLKGNTNTSTSAHKMNKNQSCSHDKQETDKCHATSEYDNITNILFLDPNTTIQHLFPDNSSATDSTTASATGSATGSAAPQNTTSAYNENNGNKANQNNIQLLFPAQEEVPSCNLTCDDHLNSTDKLQNQQRLEHSKILDTQILNDFINISSMYDFPFSKHDHEHANKILPQSHEDCHHNPHHHHQQQQQQQQQQQQQQQQQQHTHTHNLLNSTISRGQSPATASNNSTYGASITSSFNSDNGALSKEPNIDLQGFLDTNHEHAKNIQEDSQPHNHQHHHHRLEIHGHHAPFNPSINTAQPIVNTNFQSPPVVAQPFSPHQGQFTNGVQPMNNTPTQSHYHYHPQSHMPGQNLVSNCHGLHGHGNESNVETNQINFHWTNKTFGTPSSTASETQTDILCKWDGCGKQFGSLIALQAHILKEHVGTQYFDSPFNEEGQVPPQPQPVMAPPTSADTNFGFPQHSNNSGLNTEFQNRQTPSLLETELNKDTLTGYGKESQNICSWMDCFYESSNIYDLVNHVNTEHGIGFQMDMGSSNINMPHETQNKPHSSMHSHYPSLHNHQHDHAHNSVSSHNSVNSGSMISTDGARSISVEPFQDPDHHCTTECHSCKWKDCGMVFKSCKELDAHLEEAHLEKGKSFYQCEWDQCGKQFKQRQKLQRHLKVHSCYKGYQCNVCKKFFSTLDILSQHSKIHDPEKMKIQNAVRVKSPCCKKCDIEFDKNEDLKKHSQLHKNLRSFTCNKCGKVFKEQSNYIKHMKTHNYKFKCSFCPKSFTKQDSLERHLAKFHSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZP7</accession>
    <name>A0A1E5RZP7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phospholipase D1</fullName>
            <ecNumber evidence="3">3.1.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Choline phosphatase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Phosphatidylcholine-hydrolyzing phospholipase D1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AWRI3579_g1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14445"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.4.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phospholipase D family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.4" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RZP7">
        <property type="interactions" value="138"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZP7"/>
    <dbReference type="InParanoid" id="A0A1E5RZP7"/>
    <dbReference type="OrthoDB" id="14911at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01254">
        <property type="entry name" value="PH_PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09138">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09141">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000011">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896:SF76">
        <property type="entry name" value="PHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00614">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PH" evidence="12">
        <location>
            <begin position="460"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="13">
        <location>
            <begin position="723"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="13">
        <location>
            <begin position="1023"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="338"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1176"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1396"/>
            <end position="1430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1453"/>
            <end position="1491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1576"/>
            <end position="1621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="48"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="80"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="100"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="338"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1184"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1201"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1398"/>
            <end position="1419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1458"/>
            <end position="1469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1585"/>
            <end position="1598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1600"/>
            <end position="1619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OEJ92421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1694" mass="195542" checksum="D8727D734D0932F5" modified="2017-01-18" version="1">MPLQEINGGKNVNTPVVDGVGYQQTQLEKKDEQTAPSTESVVSPTAEHPNDSARVSDGETSTRSRYSTVFLNERHRNQDSSEEEDEESVQEYEPEEGNANFANNSTHGSLKQQQHGWDRSKSGRKNQAQQACAEDDDEEAKYKNLSFHGITNSLRPNFNNTGRRRSTFHELSSLGTAVKKKFQNTSQQSKNMESERNNLSKYVQERYDDSENVRDMSSDLIDTLIAGCPAALLSMTHILQDQRNIQRVPLLLNILSVHVNPVKSTESLHSAKFKIDLEYGVGPNRMKWSIYKKYKDLVSLHGTFKSMILQTTMENKLHKEKNTRPLIIPKFPRAFKNDFKNSHTHNEKKHADSRXANSTTMDQSSDVLSASSSNSSFVSVSPTMDQEVFVPQRENIFFKHLGKFNHSDETDDPNMPLNERLEKYLKILNLALSLRPAANQLFKFYEISPISVLLSYETGYFGKQGTLIMASSAKSNGWRVSHFRLNDWKAMVQRHTSKWFLVRHSYVMFVSDIHSTTPLDVFLVDAHFKIKTPYDKEVNFLEKAQNLGDDITTKQKLPNKFQLTLQNGERNMKLLFKSENLMKEWLNSITEMTRQTIWKGKNRFDSFAPIRNNCYCKFLVDGRDYFWAISEALTMAQDCIFIHDWWLSPELYMRRPAQGNEEYRMDRILKERASQGVKIFIVVYRNVGATVGTDSSWTKHRLLHLHPNIHVIRSPNQWLQNTYFWAHHEKIAVIDYTIAFIGGIDLCYGRYDTPEHVLRDDDESLVKQIFPGKDYSNARINDFYDLDKPFEDMYDRKTVPRMPWHDVHMMTIGEVARDLSRHFVQRWNYLLRQKRPSRPTPLLTPPSDLTEEELRNSPLFQLLQNRSSCECQLLRSSGSWSLGLKKTEHSIQNAYLKMIETSKHYIYMENQFFCSSSEDDGIVIENKIGDAIVDRVIKASQINQKWKAFILIPLMPAFPSQVDKPDGSSLRLIMQCQYMSICRGENSIFGKLRKLGIEPEDYIQFYSLRNWTEIGPNKRLVTSLLYVHAKVLIVDDRSCIIGSANVNERSMTGNRDSEVAIIIRDTDLVKTTMDGKEYWAGRFAWELRQRLMREHLGCNVDLIEFVERKFESFQELGAKHFKTLHFLEKKSGSKLSKSKCMFSASIELALRDVLSVDYTSAWEKVHGKIPGYGLGTGKNKESAFENSDANTGETSPHESNANDDDDNDDDDDDAEAAFNKPVSGPFESDQMFAKMXKEELPSLKRSFKSVFHSFNNRAGEANLGIRDNKKVSTDPRVNNAIVEETENFDHYDPAKPVDPNSFIKDAGXQLFEWVSRNLNNRENSKSDDVGKKFETTKIIDLLPNKEDIFSYVQNENIDDSFKWDLLKRVCYLQHLAHVEKDESRQTGSFVTDAVVDGNETKEQNEGRPHEHKKYGETKSKHSNHKRVLGKDELSNQEIDQLLSFVAMPDQLNPKIKSSESGESADASGKNDFINEKHSGDSTGSFKQKKKTPSVTDMFKKATFIDPYSFSDPLVDSFFQDLWFSIAVRNTYIYRLIFHCQPDNSVTSWADFHEYEDLAREFDDLQKEIEQIEESEAMKKTGQKGGNKSQKNGTNNKNNTEYEDQDEDEDQDGEDEEDFSFNDTNERMRSFRLRLSRGMLNGLDRKTFDKYSAKRLLQRVHGHLIIMPYNWMEQEVQNDNWYFNADRVPPIDIYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RZT5</accession>
    <name>A0A1E5RZT5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">3-ketodihydrosphingosine reductase TSC10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g45</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RZT5"/>
    <dbReference type="FunCoup" id="A0A1E5RZT5">
        <property type="interactions" value="121"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RZT5"/>
    <dbReference type="InParanoid" id="A0A1E5RZT5"/>
    <dbReference type="OrthoDB" id="10267115at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047560">
        <property type="term" value="F:3-dehydrosphinganine reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006666">
        <property type="term" value="P:3-keto-sphinganine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08939">
        <property type="entry name" value="KDSR-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045022">
        <property type="entry name" value="KDSR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013968">
        <property type="entry name" value="PKS_KR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43550">
        <property type="entry name" value="3-KETODIHYDROSPHINGOSINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43550:SF3">
        <property type="entry name" value="3-KETODIHYDROSPHINGOSINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08659">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="284"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Ketoreductase (KR)" evidence="8">
        <location>
            <begin position="15"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ92461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="325" mass="36630" checksum="5D5113FE8539EF02" modified="2017-01-18" version="1">MFSRSHNFEIENQVILITGGSQGLGLEFAKTFLKTTEKTKVIIVSRSLKKLSNACEKITSELSHLQNNSKSKPFTTLSEFQNGNTTRLYYHPCDLSSVVDVETNLQPLLLNIPVSQVFFVAGGTSPRLFKDHSLEELAQGVSMNYLSALYLSKVILSRISTCNHLVFFSSEVSFFPFVGYAQYAPLKTALKTLVCVLRQEFPEKRLSLVYPGNFESEGYSEENLTKPEITKEIEGSSVPISCEECCGKIINSLKHGYDDITTDFIGWVLMSLDMGLNKNWNRSYLWGLQLILGVVANLIIVPIYMVFVNYQIRSWLKKTGTKKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S088</accession>
    <name>A0A1E5S088_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Vacuolar basic amino acid transporter 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g160</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5S088"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000373">
        <property type="entry name" value="Vacuolar basic amino acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF198">
        <property type="entry name" value="AZOLE RESISTANCE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="199"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="289"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="330"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="431"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="455"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="486"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="531"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="596"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="77"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="22"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ92428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="639" mass="69785" checksum="72C04F5F116F40F4" modified="2017-01-18" version="1">MTSASLEKSSXSESINKKNEAQCISSRDTSNELTNASNSLDEKDLQKDYEEESVTSKEVIPXPGPPEYYEGWRLAVVQLSLNLILFIAALDIVIVTSAIEKISEEFHDYAKSGWIITGYSLPTSVCCLIWSRIAQRLGKRLSVAIAVVIFEAGSLIVALSNSMNMLIGGRVLAGIAGSGIQVLMFLVGATLVPEQKRPFVLSLLSFSFTVSSVVGPFIGGAFTNAPSGGVLSWRWCFYINLPIGFTALAVFYFAYDDHDTSILSIIRYWKANFKRDVKKLKTKNLYTKLMLELLVSFDMIGFISSAAGYILALLSFTFSNSDDYSWTSGLVIAFIVVGMLLIIFSFLFEYFLYSKLVGFLEQKYSGNEALAFNFKAAAPLFPKEAIRNIYIGCANATAVFVTMAYGFQANYVIQYFQLVFHQSAMKASINFIPFMVSISIVVFLSALVMSKTGAMKPFIILGGVAAVLGNALLSTMDGSSNEAKKIIYLIIAASAFGFVAQSTLLSSHLQLDKTDPMYMLKFAGVTGVNSFAKTMGISIGSVLSTMTFNFSVINKAKKLSPPVTITANTIVSYINGNYTSPTSTLSNMVSSSIHNVFYVSTAXAAVALILSLFTSNKRVMTSKKAKQIEKQEEEQDLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5S0A9</accession>
    <name>A0A1E5S0A9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein PES4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g174</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000001">
        <property type="protein sequence ID" value="OEJ92448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5S0A9">
        <property type="interactions" value="88"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5S0A9"/>
    <dbReference type="InParanoid" id="A0A1E5S0A9"/>
    <dbReference type="OrthoDB" id="1749473at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="171"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="259"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="386"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="133"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="6">
        <location>
            <position position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ92448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="604" mass="68189" checksum="04667A3F5216EFDE" modified="2017-01-18" version="1">MPXFTSKPKQKVQHSTRFKHSWKHIVAESGSSTYQSXVPQNSIFFESEEAHMKQSNLGSPSLQSEYRANYTLTNAVSPVFRYKTSSAKSTGKSAEEVVEGSLTLAESDSASTSSSSEDAVILCPKMSPPRTCVQPTAATSTNKQTKGNVGHKKSSNKKLLIHKEIAKNSVVPIFIGDLDQKXDEKXLLAHFQCFKSIESVRICKNPKSSTALNYAYINFKDXVEAAELLNQFNYKPIMGKDVRMMYSFRDKSQRQQMGTNVFFSELPLENENLTTRAFYEKLLSFGSILSCKLDKRKGIGFVYFXNKTAAIAAIDHYNNNLFFGQHIKCGIHFEKSVRNQCDFDEILKSSSENKVFKEDIDIATVNTCNKSNVNQINVESFVQSSSSVSISNVFKSVQLDEILDHFCQTGPIKSIYISKKKRSRNTTVVYITYKKTPDALQCIKLFDGSQFQGXRIQVKLSLXKSTYLSLIKKFQNITISEDKSDRANLKLEENSAMNLVTGPKYDIENYKQTVYFKNLSNLCDSRFLTNFCAMNSIQTTGIVVDMVNLENRTHAGYVCCGTKQDSEKLFALLNNLNVGKXPVQCSWKLDVSXKYIYKDEQLXY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0G6</accession>
    <name>A0A1E5R0G6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF1746 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4607</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0G6">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R0G6"/>
    <dbReference type="OrthoDB" id="4068068at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="141"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="276" mass="31494" checksum="D1251549822B254F" modified="2017-01-18" version="1">MDTQLSTAQRQCYMQRKTLFKKVLITDLTKTCYVVLLFKFFLFADSTLVLIVRLVATRMIKEFYPKAREGMAEFVRTGTSXGRNASPSFRLGLPGVNLSGSLTGNAERANDNENDAQSGTSQRNNNELSTAQQQQLMNEKFVVPLSTFLFHVTVTLNVLIIIYRLVFPIDFTAFNEKKRFTLMIDLLSEYHDFSTYNNFTSNLFNIFLEILILLIQFSVLQMTCIDDPKDEVDEGEPLQIESQHNEDGFDGRVHVASINIAESFEKLRARIRIASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0L3</accession>
    <name>A0A1E5R0L3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">N-terminal acetyltransferase B complex subunit MDM20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g4464</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0L3">
        <property type="interactions" value="63"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0L3"/>
    <dbReference type="InParanoid" id="A0A1E5R0L3"/>
    <dbReference type="OrthoDB" id="3971534at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019183">
        <property type="entry name" value="NAA25_NatB_aux_su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09797">
        <property type="entry name" value="NatB_MDM20"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ80438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="827" mass="96054" checksum="FAF43F5D4EF62F6A" modified="2017-01-18" version="1">MSSRQTLNKQVEQVARVLENSGSIKTGLSICDSNLQDETLSSNYKILFTTFKLLLQLQDGNKMEFGEIEKTFNNEIYMAGDTNKLIKNVPSDSMTLNWIGKLSLQLMTYDQWYKIIELSNFKNRSLEKLTVSFNDFLSKASMKNLYQVTSILGNLTKNDLVHGRNFVFWNAVISLTAIHLCAKQNSSCENVLASGAPLSWLDIDHVISKVEMSKPFQSDQERIIYCQLCEMKGDADSKKKIVAEIQPQIELQNGNIDLYLKNLIINHCEDSALLKTVCIKLLEKLNDYDILLKLINCCYKELHMSKNETLDILNSNXKFSQSRNFKLGKLEVDLLFNANNAVSEENLQSYLCDFXSKPCCFVDLQKFIKKDSSLINKLEKILKQEANKILNLNENDPVFCYNMFKLFGCDEKYTKNLETYPEYVVDKVCASLILAGESSDSSLISECVLSCFKLEKQLEVTPNEYILHLWLVVLYTSLGFLPLAQKHYCDVLKIKNVQXDVLDHLIYSRHSSVWPVKDSVISLPTSVYDSMENMPRFIDIAFDRKSYSKIKGMFEFYGDLKMSFNRWRKYTDMLIVSRLFNDTKLRKEFYNDLQQLFEFNHNYNTVYQETTDESEIELQFADNRDFNTLFSSEQSLEKLPKFMNYCNLNKQTYIQSVFVKELMLLGFQENTKKYDKFINCLLVKNXQNQPSNITKNLTEIETWSFELHSLLHFLKNGNSENFNDIAEKLQAIPEFATGQQSQAGWYYSHYLATIMTTLKTLDSLSKKFPNEIKQLVKSQLQYYRTQFVKPHFDHYTQEIEKIKDIDAAFKQSMLEQVLKLSKTCKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0M1</accession>
    <name>A0A1E5R0M1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Required for respiratory growth protein 8, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g4623</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for respiratory activity and maintenance and expression of the mitochondrial genome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RRG8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0M1">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R0M1"/>
    <dbReference type="OrthoDB" id="4035333at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031415">
        <property type="entry name" value="Rrg8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17068">
        <property type="entry name" value="RRG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="unsure residue" description="I or L" evidence="6">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ80445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="246" mass="28176" checksum="A30D21A0EB32011B" modified="2017-01-18" version="1">MSALTNLLIKQVTKSPGGKTITDPFQKLRKLPKYLPNEHTFTELDLPNFKLQNNVFADSLKAPGRKDRITRQKTPKSYLIQLKTQKTSGHMALAPQWPQSSLMQSSYISNSRKVCENLTQSQIKQSLPQNMLLSPTFSVSTNFLQDSLNEIKDYEKVLRNYIFKELLTKRDTDNSSIDDNNGVEIIYDSTRSKVEIFKTTLLAFNFHHVPLISEAELKXFPAKLDFSKDKDLIAAIRAXLDYQHDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0S0</accession>
    <name>A0A1E5R0S0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sister chromatid cohesion protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4485</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the rad21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0S0">
        <property type="interactions" value="207"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0S0"/>
    <dbReference type="InParanoid" id="A0A1E5R0S0"/>
    <dbReference type="OrthoDB" id="10071381at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030892">
        <property type="term" value="C:mitotic cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21791">
        <property type="entry name" value="Rad21_Rec8_M_ScScc1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.580">
        <property type="entry name" value="Structural maintenance of chromosome 1. Chain E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039781">
        <property type="entry name" value="Rad21/Rec8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006909">
        <property type="entry name" value="Rad21/Rec8_C_eu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006910">
        <property type="entry name" value="Rad21_Rec8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023093">
        <property type="entry name" value="ScpA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585:SF69">
        <property type="entry name" value="FI11703P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585">
        <property type="entry name" value="SCC1 / RAD21 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04824">
        <property type="entry name" value="Rad21_Rec8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04825">
        <property type="entry name" value="Rad21_Rec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad21/Rec8-like protein N-terminal" evidence="6">
        <location>
            <begin position="4"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Rad21/Rec8-like protein C-terminal eukaryotic" evidence="5">
        <location>
            <begin position="566"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="247"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="359"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="248"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="612" mass="67983" checksum="790BECA6CCD7CE6A" modified="2017-01-18" version="1">MSSSLTSNGVLAQIWLAANLDKKFNKHQSLQTDLVSSSKEIARAAAGNEEHAEQLGLRISGHLLYGVVRIYSSKTKYLLDDISETLVKLKSAFKANPKQVTQAVGSTVVSNMNNLIMQDTITAAELFYQEPLDFLSNQEIPEGLLHRGLQGAATGTFSTERNRSLAGTGAANFNAGAIPNIESSTGNHIDYFEDSLEVGRGYAINLLNDDEIEKEMFPNGHIDDQDFDLDFDISGQSQENVLGEGTAKSLNSGIDTSDPSQHGTIAEHEESPNHLLSMEHSVELGRNDEGENNEQDPLFDMDLGLDYTEAPNDQDSVDGRSLSFEQQANNAXHATQKQVSPFFSAKSILKNTKEILKDDQIEISPNEDESDNENAMLRTPTNSPPRKEKSHGGKRLLEQMYEQTYDYLPQTMYQTLLQLETHLPKSKRQKHNASLLNAPPSPVEDMHSEISLGSINREGIDISLDLNNVHESSMHIDGNDFNMDIENEISEGRTSDIDMHEHSQVEGRTTFFGENYQDDQIMQLDGQMVSKTTVEVANKLRQKLENNDKATLSELLQPRDGKQQVTKLEASKTFFQLLSLATADCISVEQSSVFGDISIREKSGLYQKYFGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0V3</accession>
    <name>A0A1E5R0V3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hsp90 co-chaperone HCH1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g4439</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0V3">
        <property type="interactions" value="97"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0V3"/>
    <dbReference type="InParanoid" id="A0A1E5R0V3"/>
    <dbReference type="OrthoDB" id="567237at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.20">
        <property type="entry name" value="Activator of Hsp90 ATPase Aha1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036338">
        <property type="entry name" value="Aha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015310">
        <property type="entry name" value="AHSA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 HSP90 CO-CHAPERONE AHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009:SF15">
        <property type="entry name" value="HSP90 CO-CHAPERONE HCH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09229">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01000">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103111">
        <property type="entry name" value="Activator of Hsp90 ATPase, Aha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Activator of Hsp90 ATPase AHSA1-like N-terminal" evidence="2">
        <location>
            <begin position="12"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="149" mass="17441" checksum="5DC4D87304568E3B" modified="2017-01-18" version="1">MVLNPNNWHWIEKNTLQWSKEYLETTLVNFQVPVKDDKYSLVYVKEIENVTGDSNVSQRKGKVICYYDMDLKFKIGVRPRDAIDETEDVFGTLFIPEFMHDDMNLXVKMDGFQNDXKGLVDENFINLFSSKLLNYQNDLISAHTKGIQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R0X7</accession>
    <name>A0A1E5R0X7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome b-c1 complex subunit 6, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Complex III subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4553</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UQCRH/QCR6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000012">
        <property type="protein sequence ID" value="OEJ80559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R0X7">
        <property type="interactions" value="125"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R0X7"/>
    <dbReference type="InParanoid" id="A0A1E5R0X7"/>
    <dbReference type="OrthoDB" id="405848at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.20:FF:000003">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.20">
        <property type="entry name" value="Ubiquinol-cytochrome C reductase hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003422">
        <property type="entry name" value="Cyt_b-c1_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023184">
        <property type="entry name" value="Ubol_cytC_Rdtase_hinge_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036811">
        <property type="entry name" value="Ubol_cytC_Rdtase_hinge_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15336:SF0">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15336">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX 7.8 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02320">
        <property type="entry name" value="UCR_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81531">
        <property type="entry name" value="Non-heme 11 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ubiquinol-cytochrome C reductase hinge" evidence="12">
        <location>
            <begin position="59"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="17"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="25"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ80559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="130" mass="15254" checksum="750FC2A2F5853EE4" modified="2017-01-18" version="1">MIASITEYLEELKEAFVPTVAKAEEDAEDNNDEAGDDDEDXDDEDDEDEDDEDEDAVVDQFDALREELRNTEKGKELNHHYMECVERVHKAQEDPNYAEAEYKEDCVEEFFHLQHYLDEHAAPRLFDKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R158</accession>
    <name>A0A1E5R158_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4302</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R158">
        <property type="interactions" value="257"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R158"/>
    <dbReference type="InParanoid" id="A0A1E5R158"/>
    <dbReference type="OrthoDB" id="3971650at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097035">
        <property type="term" value="P:regulation of membrane lipid distribution"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000749">
        <property type="term" value="P:response to pheromone triggering conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05574">
        <property type="entry name" value="STKc_phototropin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000524">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000121">
        <property type="entry name" value="Serine/threonine-protein kinase nrc-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45637">
        <property type="entry name" value="FLIPPASE KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="696"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="28"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="109"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="175"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="285"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="357"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="459"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="550"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="634"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1013"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="111"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="177"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="292"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="370"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="396"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="470"/>
            <end position="480"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="550"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="634"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1019"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="13">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ80645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1143" mass="127942" checksum="E9F2D12D7944006A" modified="2017-01-18" version="1">MLNINILGGKENAASXDKVFTDKHDTHDKEYTQEEPLKPPPFAFNDLDECSNSTHSFNSRASSNSNXHSNGSPPVNGHAFTDVFVEENFDEQDCSNDANFMENHKFKNYSNHHTNNSSATSNAANSRKSSVNASLNSTNNNNNTIGKKVRSVSFGKILPWSLRKFSSNSNLNQVMKHHQENDSTSEDKSEGEXPATTTTTTTAKDEFDTKDKXLETADLNNLGSPANSAIEXNDEKIVGMMEQNNEHEDTQNKMLEENYYETIVPLKENIHVSLSVGSSTSEIGEQINTNEVDDKTNXNDSKLNKSEKEKFEDKXNISDEQLEPSDSAHTGDSAKVATKSSGRFSLKKLWGSNDTSKIARVRSPSAPVPLNSQTKNTQQNSDTSSRDATKTTTSTIKNKVNNIVSSNNNNSTNNGTCVSGGSNNNSRSNSISNLSLGDSFSSLSLFSKSKMFFSNKNNSSSAIKSVNESSDIEAVPEEIEFPSSPVTTAESSELGEKQMKANXLKEASKNPHVYRNIKATHTSTITSNIPTSPMRKTRPRSATQSILQTPLKNQKHTSMFSSSSDLGTMTTPKGKHDSYQRSTISTTTLKSTNIDVTDTASVSQAKDSNGSTFSGPEFLKQRCMSEQILSFSTKKNATSNSEALSSSQQEEVGQSTEEQDLLLNLPKPPEIKRSTRLRSRSFGNKFQEVIVGPQSFEKIRKLGQGDVGKVYLVREKKTDRLYALKIFDKHEMIKRKKIKRILAEQEILATSNHPFIVTLYHSFQTEDYLYLCMEYCMGGEFFRALQTRKTKCIAEDDARFYACEVVAALEYLHMMGFIYRDLKPENILLHQSGHIMLSDFDLSIISTTNFKAKNPTMTKSSSIWSSMLDTKVCMDGFRTNSFVGTEEYIAPEVIRGNGHTAAVDWWTLGILIFEMLYGFTPFKGDNSNQTFSNILKNEIIFPNNNTVSNTCKNLIKKLLMKQDFKRLGSKSGASDVKKHSWFKKVQWSLLTNQEPPLIPVLSKDGFDFKKLSNNKKKQQQQQQQQQQQQQQAQEDLNVQAENRSSDEVADLQTTVSDVISYGDSIEKNMFEEKVEFDDCITDDNPFHNFNSMSLMCEADQLKLDDSPPLYGNGDTTFYGKINYTPNENRSRSNSHRNIFFKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1A1</accession>
    <name>A0A1E5R1A1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein mS41</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Protein FYV4, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4005</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS41 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1A1">
        <property type="interactions" value="147"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1A1"/>
    <dbReference type="InParanoid" id="A0A1E5R1A1"/>
    <dbReference type="OrthoDB" id="18595at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039603">
        <property type="entry name" value="Ribosomal_mS41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019083">
        <property type="entry name" value="SAM_Ribosomal_mS41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28235">
        <property type="entry name" value="PROTEIN FYV4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28235:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09597">
        <property type="entry name" value="SAM_Ribosomal_mS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01238">
        <property type="entry name" value="IGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small ribosomal subunit protein mS41 SAM" evidence="6">
        <location>
            <begin position="34"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="135" mass="16170" checksum="D9C275F1A9AAE244" modified="2017-01-18" version="1">MIGRRFFXSTALNLIKSTNINRPIPMPTAEIQDVNSFLTKIGRGCDEFAPLYENNWENLFKWKTPELKEKGVSVQQRRYILHNVERYYQGLKITEMKKGKKSFWGGERRRPETIAKWRAQERAKEHEEFMKQHKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1C7</accession>
    <name>A0A1E5R1C7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-rRNA-processing protein ESF2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">18S rRNA factor 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Pre-rRNA-processing protein esf2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g4315</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESF2/ABP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1C7">
        <property type="interactions" value="975"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1C7"/>
    <dbReference type="InParanoid" id="A0A1E5R1C7"/>
    <dbReference type="OrthoDB" id="287393at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034462">
        <property type="term" value="P:small-subunit processome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12263">
        <property type="entry name" value="RRM_ABT1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039119">
        <property type="entry name" value="ABT1/Esf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034353">
        <property type="entry name" value="ABT1/ESF2_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12311">
        <property type="entry name" value="ACTIVATOR OF BASAL TRANSCRIPTION 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12311:SF7">
        <property type="entry name" value="ACTIVATOR OF BASAL TRANSCRIPTION 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="295"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="349" mass="40374" checksum="13966C1F96E9422A" modified="2017-01-18" version="1">MSTSEHESDFSNDEEDQQQSLYLSNKKKSVNDVFNXIXNEDDADDSDANFSEEEEEEDHALVDPSESFSKSANAKQAQHKDNQSRDDSENESEDSIEREEKGSNDNXDNTDEEETVPANQSKKLDEEQLKKEKLERLKKLKSQKKSKHKTGVVYMSKIPPYMKPAKVRQVLSRFGEVDRLFLKREDETKHKRRVHSGGNKKIMFEEGWAEFIRKKDAKLCATTLNGNIVGGNKRSFYYDDVLNIKYLSGFKWSDLTEQIAKENDIRQSKLELEISQANKLNAEFIRNVEKSKMINNIKKQKNSNKRSNNEERNNNAIKQRKIVSNRLNAPEHTKQGTSXELKNVLSNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1D2</accession>
    <name>A0A1E5R1D2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Sorting assembly machinery subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g4098</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1D2">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1D2"/>
    <dbReference type="InParanoid" id="A0A1E5R1D2"/>
    <dbReference type="OrthoDB" id="198787at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021211">
        <property type="entry name" value="SAM35"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10806">
        <property type="entry name" value="SAM35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ80714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="350" mass="40646" checksum="B7FC2E6B21D02067" modified="2017-01-18" version="1">MSSYTPSIVTKTFSVFPLSVYPGQHDKDNKLQKEIEKHTFPLRSRXQQQDRNXQLVEKHEDSIVIGVYKVKEYREMMKNTTVYLPTDPLCLNVLLSMMLKNDLLLPKSTCFQHSKYSVVELSYHASPDGALPMLVEPERLDSKLKIHTRNPIIRTYGEINNVLAERLSSENDGHLLLAELMDTIFYDVFIMQLLKEPEVYASKYVLDSSALTQQQNYNKFMLNLMNQNRFSIRNQAIHNEFNREFIFNYARFEQRVMFSKNKCVTDCKDLLEQIEKELSNNESNIFXKNSADMEENPFTTTNSPSYLDLKLASYIVPMLSLGVDNDLSCFLEKSCPLSTQRAKHVISYFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1I3</accession>
    <name>A0A1E5R1I3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Purine permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g4335</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1I3"/>
    <dbReference type="InParanoid" id="A0A1E5R1I3"/>
    <dbReference type="OrthoDB" id="1641903at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042907">
        <property type="term" value="F:xanthine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006042">
        <property type="entry name" value="Xan_ur_permease"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00801">
        <property type="entry name" value="ncs2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42810">
        <property type="entry name" value="PURINE PERMEASE C1399.01C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42810:SF2">
        <property type="entry name" value="PURINE PERMEASE C1399.01C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="92"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="349"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="436"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="464"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="496"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="530"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="599"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="614"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="9">
        <location>
            <position position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ80761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="624" mass="67289" checksum="9A41F41EBE0262E2" modified="2017-01-18" version="1">MDSILENGSTYEVEEENNPFVTTDFSKESFLNFWKKAYKKVTTKEGLIGDYDYKYLFTPSFPFHDLFYKKVLKREPITVEQPFFSLNSDIPVVLGLLLGFQHALAMLAGVMTPPIIIASXANFDTQLQEYLVSASLISSGILSFIQITRFHIYKTPYYIGSGILCVVGTSFATITVISKTFPLMYSTGFCPVAADGSYLPCPDGYGAILGTACVCALLEILISFTPQRILARLCPRIVTGMVVLLIGISLVTTGIQDFMGGSGCVGAACAARNGNTKPWGSAEFLGLGFLVYIAIIFCEKWGSPIMKSCAVIVGLLTGCIVAAACSYFSSADIDAAPAATFLWVHTFKLTVYGPAVLPFLVVYIVIAQECIGDITASCDVSRLDVSGPAFDSRVQGGVLADGISGVISGLMTLTPMSTFAQNNGVIAITKCAARSVGYYCVLFLCVMGIFTKFAAALVSIPKPVLGGMTTYLFCSVAVSGLKIISTISFTRRDRIILTASLMFGFAANLQADWFSYVFTYSGSNHALSGFFDAIVLIMESGFAVSGIIGIILNLFLPQELDEELIDQIEVQQSNLAVLEAHESPQELYNVLSAKSMEAAGETSRNNDKEKDYINVTTSSQRSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1M1</accession>
    <name>A0A1E5R1M1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ribosome production factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3992</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R1M1"/>
    <dbReference type="FunCoup" id="A0A1E5R1M1">
        <property type="interactions" value="961"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1M1"/>
    <dbReference type="InParanoid" id="A0A1E5R1M1"/>
    <dbReference type="OrthoDB" id="264354at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10480:FF:000002">
        <property type="entry name" value="Ribosome production factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10480">
        <property type="entry name" value="Probable brix-domain ribosomal biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044281">
        <property type="entry name" value="IMP4/RPF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22734:SF3">
        <property type="entry name" value="RIBOSOME PRODUCTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22734">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN IMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Brix" evidence="2">
        <location>
            <begin position="122"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ80805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="324" mass="38059" checksum="94DD2FD859163412" modified="2017-01-18" version="1">MGDIDIKNKQKRQEVFAKIKHQKNKERHTSRKQRAKEELENPELKAKRLKENVPRTIENLRVYDETIETPAETATSGKTAEDSEADEEETTRKEDEKDESQLEGDGMDELMKLFNNTANAPPKILLTTNVNARKVAYEFANIMIEIFPNVQFVKRKFGFKLKDIVQFCNNRGFTDLIIINEDKKKVTGLSFLHLPEGPSFYFKVSSFVEVEKIPGHGRPTSHIPELILNNFQTKLGKTVGKLFQTLFPKNPDFQGRQVITLHNQRDHIFFRRHRYIFRNEEKVGLQELGPQFTLKLRRLQRGIKEETEWEHKPEMDKEKKKFYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1V5</accession>
    <name>A0A1E5R1V5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">3',5'-cyclic-nucleotide phosphodiesterase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g4379</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyclic nucleotide phosphodiesterase class-II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1V5">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1V5"/>
    <dbReference type="InParanoid" id="A0A1E5R1V5"/>
    <dbReference type="OrthoDB" id="3972433at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004115">
        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047555">
        <property type="term" value="F:3',5'-cyclic-GMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006198">
        <property type="term" value="P:cAMP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902660">
        <property type="term" value="P:negative regulation of glucose mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024225">
        <property type="entry name" value="cAMP-PdiesteraseII_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000396">
        <property type="entry name" value="Pdiesterase2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28283">
        <property type="entry name" value="3',5'-CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28283:SF1">
        <property type="entry name" value="3',5'-CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02112">
        <property type="entry name" value="PDEase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00388">
        <property type="entry name" value="PDIESTERASE2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00607">
        <property type="entry name" value="PDEASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0114">cAMP</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="171"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="5">
        <location>
            <position position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ80888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="469" mass="53551" checksum="668BCB75CF04A5EF" modified="2017-01-18" version="1">MLTVVILGTSGGPFEVNTQSFLVKQNDRSYLSVDSGCGLSSICNLFRKRGYEQNVNLYETFDSYSVEDSTEKKWEEYRRKVVQLTDPDVNVSFKEPENVYGENLMLKTANFYSYISNYFITHPHLDHISGMILNSPFIFDPYFFSKGLEVEGHASXMSSLNXTKSAAVGTKSANSNNNNKRTSSDRGSSVVEKPKGYDIVKPLQKTLFGFSNTIKSIETHIFNCEIWPNLLYKNEEFLKTQIIQPHEKIAVPGQRKLHSKPSIRSGNRIGEKEEDKEEAKCLFEVTGFPILHGRNVMNPKACVLSSCFYIADKSSKESVIMFGDCESDSIYLPKLWDFLADEILIREFMLKGIFIECSTVDMINVGFELLNTDKLYGHFIPYTLCQELLGLKNIIDQKLQTSNKQHSISHNPYENVFPINVFITHVKQTVTLDDPRYTILKQLKEHAVENHIDDLFKFSIALNSHTYXL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1W2</accession>
    <name>A0A1E5R1W2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SNF1 protein kinase subunit beta-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g4323</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 5'-AMP-activated protein kinase beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1W2">
        <property type="interactions" value="454"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1W2"/>
    <dbReference type="InParanoid" id="A0A1E5R1W2"/>
    <dbReference type="OrthoDB" id="531008at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140767">
        <property type="term" value="F:enzyme-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000562">
        <property type="entry name" value="Snf1 kinase complex beta-subunit Gal83"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.250.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006828">
        <property type="entry name" value="ASC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037256">
        <property type="entry name" value="ASC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050827">
        <property type="entry name" value="CRP1_MDG1_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343:SF84">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04739">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01010">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160219">
        <property type="entry name" value="AMPKBI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Association with the SNF1 complex (ASC)" evidence="6">
        <location>
            <begin position="451"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="55"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="132"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="297"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="389"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="509"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="77"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="90"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="174"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="192"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="297"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="346"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="406"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="443"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="509"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="526"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="542"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="7">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="unsure residue" description="E or Q" evidence="7">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ80895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="622" mass="68889" checksum="7C75F2A086DFF92B" modified="2017-01-18" version="1">MSSNDNTHDSMQGQQHKDVSMVDVNTLHESLSQMSIDDNSFGSMQGFAPLTRQNDTQTSLNTQSTINSTSHTPQPSVQVQNNNYQQQVQTDFGNNGGNAATSSPLPPLGRKRSTMIFSDDYDVPEFRENADTVNDISDDERLHHDGVHGVDPNSQTXQQKQGAGTSYTSPNQQSSSSSSSSASFPTRPGSTQQQQEQQQQQQQQHDRPVLVPVSIKWEQGGNSVYVTGSFTDWRKMIALTKNQEEPHIFSIVLQLPPGNHRFRFIVDGELRISDFLPQATDQLGNIVNFLEVSSPYSSTAMANSSNNNTVVAGPTNTSNNNNNNNNNNNTLARPAGEGSPEYPHKQAGQQNNVYQQQTDQXQLDQQQQRGQAANANKRHAYKLAGSGFTSLDDETKESSSDKQHQHAQMSKSSRLALQIAAENDQEPEDIGNGFSRVRPGDNKPLLSSSASSKAQYQYTTDIPAVFTDPHVMEQYYLTLDQQRNNQQYMSWLTPPLLPPHLEKVILNASYSTPNPNNLPKQDSNGHQHRHHHHHSHHHRTHQQNSIGSSDSSLSSSQMSVEKQPSPQPPANSDENNSGALPIPNHVVLNHLITSSIKHNTLCVACTVRYKSKYATQILYAPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R1Y2</accession>
    <name>A0A1E5R1Y2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="7">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g4285</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="7">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="7"/>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R1Y2">
        <property type="interactions" value="901"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R1Y2"/>
    <dbReference type="InParanoid" id="A0A1E5R1Y2"/>
    <dbReference type="OrthoDB" id="3370at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070013">
        <property type="term" value="C:intracellular organelle lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17956">
        <property type="entry name" value="DEADc_DDX51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="278"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="489"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="112"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ80915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="687" mass="77533" checksum="7EBE51A2614140E8" modified="2017-01-18" version="1">MFAARFVPGAIESKVSKKNTEKSAPKKVLPLKRSREITEKDHEEEESMDDESSDEDSSEDSXKDSSEDSSEDSSEDSSEESTTDDSNEDKQGKEGLSEQISTIKDKTEIVLNEQNTNDDTDKSSTLTETKEYSDSKHSSVLSRFKQTLTIQDELQKKNKNKGLEGAXLEEYNDQQGAQEDADVVLHDLAPIPQPAKIYSNAHDSATSLKLDTSTAWSHTTKIIYNNGMIKSWEDLPLSKLLVKNVKHNFKSEKAFPIQTSLITTVLPLLQFNYNINKRMYPQRTGDILVNASTGSGKTLAYALPIIEILKKRTINRTRCVVIVPTKMLIHQVFETFNKLTQGTSLVLTTTKLEMSLKEEITKFEKQQPDILIITPGRLVDHINSGSISLQDLKFLVLDEADRLLNQSFQNWCDLVMNELKKHDKLNNVIKMVFSATLTTNTEKLHHLHLNTTNTKLFIMDSIKLYHIPSSLQEHNIKISSSKSVYKPLYLTKVLKNLNSISNSLEKAFKVLVFVKSNESSIRLSPLIQALWKENESQISSINSNNSKQENKVVLERFSKTQTFNLHAGISVLISTDIMSRGIDINDITHVVNYDVPISSQQYVHRSGRTARALRSGDVYNLLVGRGEYNFWKNQIDIDLSRDVGGCEVVEEDTTGNSDAALEENHYLSLNELEVAEYKKCLQSFSSX</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R202</accession>
    <name>A0A1E5R202_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g3893</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R202"/>
    <dbReference type="InParanoid" id="A0A1E5R202"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ80935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="70" mass="8013" checksum="F58CFDC9F980D4C1" modified="2017-01-18" version="1">MLPSHAQVSRDVGICCSRNMVFAGKKFSDIEIRRDNDDDDNDDDDDDDDDDDDDDDDDDDDDDDDDDIHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R306</accession>
    <name>A0A1E5R306_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Putative nucleotide-sugar transporter YMD8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g4145</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000011">
        <property type="protein sequence ID" value="OEJ80953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R306">
        <property type="interactions" value="696"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R306"/>
    <dbReference type="InParanoid" id="A0A1E5R306"/>
    <dbReference type="OrthoDB" id="18894at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013657">
        <property type="entry name" value="SCL35B1-4/HUT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08449">
        <property type="entry name" value="UAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="318"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="353"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="382"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="12">
        <location>
            <begin position="253"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="431"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="449"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ80953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="482" mass="54879" checksum="F72B1933943D098A" modified="2017-01-18" version="1">MLDPKYGLXLPYPLFITSVHQFFLWIISFIYVRVSKARKLKKQGRLSQRLAASRGMGSTNGDELQITPQVITPDRKQKLVYYMKFIVPTAVASAGDIGFGNLSFKYVPLTIYTVIKSSSIAFVLLFGCMFRLEKYHWKLGVVVGVMFVGVVCMAFKPSSDSDSGDDESAWTFFFGVVCVVLSACLSGLRWVYTQIVLKKKDAAAAAIAEGAEEPVSGSITSRNTEDFPLNDVLSTQTHGNDLQTQFSGASRMTRINQKNNNILENKKKKKKKKKNPIHTINQLAPIMGFTLLLASFIFERPSFEIIFYKLFTFNTGDFNVXHFFMGISFILFPGIQVFFMTICEFGILQRSKVLTLSIAGIFKELITIWVSMLILHERIKSFINWVGMIIIMLDVCYYNWYRYMEKKRIEREEEEEQNRENDFSAIAVKSASGSTYASHPPDYQTIFDLDQDDEDEDNEEEEDELDDFHYSNLXGDGVGAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4J3</accession>
    <name>A0A1E5R4J3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3555</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4J3">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4J3"/>
    <dbReference type="InParanoid" id="A0A1E5R4J3"/>
    <dbReference type="OrthoDB" id="292964at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF24">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="639"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="475"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="874"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="481"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="500"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="512"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="522"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="874"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="892"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="912"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="941"/>
            <end position="950"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ81836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1076" mass="124508" checksum="639F9E52503770CA" modified="2017-01-18" version="1">MYTPEYSPELEALVDYAFTKSELKEQYPKEKLEKLINLLEDAQYYFEQYREWLEKTNIHDEPAKQLANFAHEDGQLXENLKGLVMFIIGCFYIYLIIPNSIQFQTRNKSFSIYTELKKLYQDELNMTNVAKMVRNYIEDHITADDLLQRQKSRIHRXRAFSVSKRQRSATFAGTDTLDQTIIDGIDAKELHMMRSQSKDYLGLNELSRLSLADKGRPLHPSHYFEPISKHHYEEYDDEDAKTITWKAPQISSNDKLQQFFSQSTNGATLPLPESYHHKNVDKDTYHSAYLWTEEDKDDGYDEVQDDLLIQLMKIKNTATITPDKLLELIANFPTETLLIDLRFEKRFASSHILAENIINIDPFMFSSYADFETPSADERELKEALNRNLDSRALTLFKRRGHFKHVIYYTDMKTFLNVGFDYQLKLFQVLYSTSSPNPTSLIGGYEQWKKFLHDKAKLNPQIDYSKYVYKPSRSIQLNDQTTSSSTISSKSNSXPLQNXQPPPLPSCPPPSLLSSRALPAIPSVPPPPVPLPSERPRMSIPAHPIATPFEASSGNFHPSTIRKDVSRSHTMSEFTSSPNFKRVPNYQKXETRTKNSYSPEGVLEKDYQYQLKVSPNKIFNFHLPTVEDNPDEFISLSIVGLKNIRNTCYINTILQCLFEARLFRNLFLSAKYKEYFSTDYQQRYQLSTSFYALFRKMYMNGGCSVMPSGFLKSCNLLRPDLNIPSSQQDAQEFLLFVLDQLHGELSNKEAVYRDLPHLLLHNDKYLHVDQKEYDSWFEKNYEMNGLSPIDEIFQGQIENCLECQNCGFSKFNYSTFYVLSLAIPAAEKVSRLHLHATSKKKEKIIRLEDCINLFTSDELLIDENAWDCPKCLAKSNSSANSSTSKELSSGHSSRRKLLRSKFFRTSKHHKSSSAADVNSESEGHNNTQSDDHDGMVSNKQKDKKKQKKKATLKSMNFISLPHVLTIHLSRFYYDLTKKNNTMITYPLILNIVLKNDTVLKYRLYGLVNHFGDLTSGHYTALVNKSLQHSIHHNSQKWYYFDDETVKLDQTHGDFDNGFSSISSKDAYVLFYEKIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4R5</accession>
    <name>A0A1E5R4R5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Exocyst complex component SEC3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3682</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R4R5">
        <property type="interactions" value="73"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4R5"/>
    <dbReference type="InParanoid" id="A0A1E5R4R5"/>
    <dbReference type="OrthoDB" id="3971932at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13315">
        <property type="entry name" value="PH_Sec3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028258">
        <property type="entry name" value="Sec3-PIP2_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048628">
        <property type="entry name" value="Sec3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019160">
        <property type="entry name" value="Sec3_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16092:SF14">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16092">
        <property type="entry name" value="SEC3/SYNTAXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15277">
        <property type="entry name" value="Sec3-PIP2_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20654">
        <property type="entry name" value="Sec3_C-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09763">
        <property type="entry name" value="Sec3_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01313">
        <property type="entry name" value="Sec3-PIP2_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec3 PIP2-binding N-terminal" evidence="6">
        <location>
            <begin position="118"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="16"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="239"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="328"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="373"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="516"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="644"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="22"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="526"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="644"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ81905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1504" mass="169291" checksum="BD1E4DA82419EBD9" modified="2017-01-18" version="1">MKKASHSRKLSIDKIRGFGNAGNKNQHVTPSTTVSNTGGRKPSFTKTHHRKTSSVNSVSSNVLAEQYDRDSEGIIKSCFAKKLDPATNQLLNQYFTHVRIIEDSRYPSSRPSQDSSLTNKKKRLLIVSFNKQLKQVQLHKARQNTDGSFQIGRTWLLKELQCVERDEQVAEGFSMTLGKIYYWETHTAKERTIFIKTLIKIYMDHFNEHVPVLKNWDLGLFYLDEQSYKRAVISPTSFSSSASQNKQKESIKNEQSQKLSINTTPTMKSSIPQQTPSLVKXTISKAAPQGPVSSQVSKPADISKEKANVLQTLPYSNQNRYVSTASAITKDHQKETPNTISNPLHFEKGNYPNFKPQQAELQQDLQQNDENAPYVDDSVKTKSKFSTSSNENPYANAQQTLASPALNSFSKFATVPEESANQLSPQKIPQINARERPTENSSAAGLFVAASPQKDSGSHTXAEVISLHSSNGDSVIESKSYQNEVEFDESYQHDMEAIPFETDTTEPLNIESANAEKDHXHVNGIDTDDDDLNNPPELDDNLSSSDHLFDHXGATTKEKTTGVMHINTNMKTVREISTLHAETDMSFEQGDEMKYAETYHTVDPSSKNEISLDTFTGSLLHSEKGNNEGDFAMADFTEXAVAHDEQKXNKKSPEINQNDNPGDEEENYDNDVITAMLETVNWDPYDTTESLLKKLRMKLTETQYTFNKELNSISDASLTTLSSSNVTNACDKIDSWLSFFSMELASFSKDIEYVETQNNYLQVESANKKKLWTDLSQILNDVSIDPLVLSSINNTPINVQNLVSLEAALSKLYTALNAINGGDQNNDLDVLGKPSGSSALVEDDFDDLSKMEALKERKALYNGVSKKFTERCSDIFENRIFGSFVQSQDSQELSKILPFASLTLFMKNVSPADFHIITETWNTKVFKVYEELCSRKLLEADQLLKAYSQKKLAGQTSTSMSSSEAATTQPLFSSAQSFTTTTSHRTAPFSTSTSFEPQQQKKTLLHYQDISSLLQAWESFKQTKKLDVLEDSSHSDLLVQLVECISYAQGLCSTYQNFVAEFFHLRSDELPFNEYIAKVPLAQRSHGLNRYLELESDRNIANSKLNMVSAVFNATLTSYFSGFASLIQRYAPSLGPGIMFYIENEINCVLLNSDQEFIINIYERFSKRLASNWDQFILEQQSLIDKTPLVMDCKKPIPVVLSFSQFFFNLHQEMKFLVNKYQNTETKQLEPNTQTAPKTFDYVEKSYYKLSVCVMTLLAKYSADASLQKLSKSKAETLPIKQASRSINSVSSMSGNIDSGESQSSAXLEARALVNRNVLLLLNSNWLIETQGLLESETMTDVFVSSCKKVFDQQKESYADIALRKAMPKLYLFVTSAWSISEEVRTNKTNADPSKYAAYSQSNLANILSVYQSRDMNQLINRLYEDMMSDFSEFQSFVTAVTSSPKFTNELYSKLWSNLQGATVSFYLRLYSLIEKHYKGTEIKFSKNDIISAFTSCKANFSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4T4</accession>
    <name>A0A1E5R4T4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine/threonine-protein phosphatase 2A activator</fullName>
            <ecNumber evidence="9">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Phosphotyrosyl phosphatase activator</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3564</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the PTPA-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="9"/>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4T4"/>
    <dbReference type="FunCoup" id="A0A1E5R4T4">
        <property type="interactions" value="105"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4T4"/>
    <dbReference type="InParanoid" id="A0A1E5R4T4"/>
    <dbReference type="OrthoDB" id="16120at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008160">
        <property type="term" value="F:protein tyrosine phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04087">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004327">
        <property type="entry name" value="Phstyr_phstse_ac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043170">
        <property type="entry name" value="PTPA_C_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037218">
        <property type="entry name" value="PTPA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A ACTIVATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03095">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016325">
        <property type="entry name" value="Phstyr_phstse_ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140984">
        <property type="entry name" value="PTPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0697">Rotamase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="347"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="352"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="364"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ81926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="452" mass="51388" checksum="4D0EBC7D090B521C" modified="2017-01-18" version="1">MNEFQLAETAFSKPAKKIFDAPTTQVFQSSVALHRLRYYIEKYCSMVEGVTYDHKIIQECISNDAPQKHPVLEKLLKYVFTPLSTQIKETPPLPGPTRFGNFACRDWHDKINKEMPLMLERLLDDFANESTVGGDTVTRKYKESLVELQYYLLNAFGSKERLDYGTGHELSFLAFLGCLDMLGLFKKISKMELLAIFFQYCALVESLILTYNLEPAGSHGVWGLDDHFHLSYMFGASQMVKNKNFQGISPKIMLADPYIVDDHYMEILYFWNIKFVKTVKKGPFEEHSPILYDISRNVKQWSKVLKGLTKMYDDEVLNKFPVVQHFWFGTGFYPWTSMNDGTKLVESEQELASDKESQKEEVSKQTQNTQTSGLSTSAAAALEKSEANVPSSSHKNAFANMSDMMASRRGANLGRNVQPGARPCPGSPGNKQNILTNMPPPSMSYPTRQSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R4V9</accession>
    <name>A0A1E5R4V9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GTP-binding protein YPT11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3762</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endomembrane system</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R4V9"/>
    <dbReference type="FunCoup" id="A0A1E5R4V9">
        <property type="interactions" value="123"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R4V9"/>
    <dbReference type="InParanoid" id="A0A1E5R4V9"/>
    <dbReference type="OrthoDB" id="3971370at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="63"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="504"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="91"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="504"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="525"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ81932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="569" mass="64947" checksum="69BEB15E5AB12CD4" modified="2017-01-18" version="1">MTTTSENSVDRTPVSSRPARANGRNNSRLRKRKGYSMMVDSSNIPSFGDPDTLQALSKSMFRDESLEKQKNSSSSSSNIVVGREGHERQRPSASRSTDELVTQRSFQSLASIRQSSTHTPKRPITGFQLRNISPLFVDTATQRHRHTRHFSPSTPKSATGNITHSLTGDEIPADTIKLLLIGNAGVGKTALILKYCNELQIRRKPMNYSAETQLLSQNIDIDDESQNTLQDFDSTLDDLENTLVNKSNQAHDPRRRNVSTNKSIKKLTRKQKCYSLSDYEELLNSSYDPRKTHNQERGKKFGPGDTNDSWNILNDYNSGESDVDMDEHDEEDMWEISTETTVGIDIKTQLINLDNRYFKIVLWDTAGQERFRQSLNPLMYKKTQGLILVYDICSLDSFNSLMDYWIPEWIRNNDYSMGRCYLIGNKLDLYKKRQVTHEHVLKFVFEAENKYANQLCIGGNFEVSCKWLENNGVESSFSRIVQDLISHGCYDLVTKAERPRKVVRGDLKDANSDSEEVKHQYLDNVTDEDEDEDEDDDEYSTMNSTKIVDLTKHKKYSSSAEDRGSGCCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R524</accession>
    <name>A0A1E5R524_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein FMP52, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Protein fmp52, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g3688</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FMP52 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R524">
        <property type="interactions" value="102"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R524"/>
    <dbReference type="InParanoid" id="A0A1E5R524"/>
    <dbReference type="OrthoDB" id="430436at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051170">
        <property type="term" value="P:import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000366">
        <property type="entry name" value="Protein FMP52, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014843">
        <property type="entry name" value="Him1/Fmp52"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14097">
        <property type="entry name" value="OXIDOREDUCTASE HTATIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14097:SF7">
        <property type="entry name" value="OXIDOREDUCTASE HTATIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08732">
        <property type="entry name" value="HIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00071738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00074024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ81992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="248" mass="26758" checksum="C5353BDF917DDA0F" modified="2017-01-18" version="1">MSNSKEYTAFVLGSTGLCGGAILKHCLGSGKFTTVTTLTRRALPEYLISPTELANQKDVLTSIVDADSQNWAXKITAPEYGKNNVFFSGLGTTRAQAHGFANQYKIDHDLNVALAQKAKEAGYNTYVLVSSVGASKDSMFAYTKMKGEIEDAVEKLGFDNLVVLRPGLLLGDRHGNKSQSFMSSAAESIGARFYRSKLQKLFGYPVYGEEVGKIAVKLALETSVNATPSGKTNVRIVESNEILDLAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R5J6</accession>
    <name>A0A1E5R5J6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 4B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3525</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000010">
        <property type="protein sequence ID" value="OEJ81813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R5J6"/>
    <dbReference type="InParanoid" id="A0A1E5R5J6"/>
    <dbReference type="OrthoDB" id="3972811at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="100"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="43"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="207"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="207"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="237"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="270"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="284"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="303"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="339"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="435"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ81813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="444" mass="49616" checksum="CBEE66361068BCA3" modified="2017-01-18" version="1">MAPPKQKGVKMDLSAFLNDESLGGSAWTDQEVDLNKIKVDLNKPSTEAASSPSSAQRGGPSLESFLHGRGSSLDPAMAGNGAPQRERERIVYEVPDYPPFKAKFVNFPLDVTENGIKQWFEDGLEKQGAVSEVYIPKNMDGTPKKYAFVTLTERQYLVESLTFNGNKLNSRTVYVDVAPPMRNREMNDRPQVNWGSARGANFGSIPEREFEHREPREQAQVNWGSARGANFGSIPERQQREFREPREPREQPQVNWGAAKGANVGSIPERPQREFREPREPREQPQVNWGAAXGANXGSIPERPQREXRXPREPREPXEQPQVNWGAARGANFGSIPERPQREQRSPREKTEAPKFNWGAARGAKLGEVKKQPSESSEEASADGKXSSMKSSFAVLTVEDDDEDEQKNKPQPQETETKAQEETPVSSEAKAPVQADEDGWETTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7B1</accession>
    <name>A0A1E5R7B1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HTH CENPB-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3487</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7B1"/>
    <dbReference type="STRING" id="56408.A0A1E5R7B1"/>
    <dbReference type="InParanoid" id="A0A1E5R7B1"/>
    <dbReference type="OrthoDB" id="3972786at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006600">
        <property type="entry name" value="HTH_CenpB_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03221">
        <property type="entry name" value="HTH_Tnp_Tc5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51253">
        <property type="entry name" value="HTH_CENPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH CENPB-type" evidence="3">
        <location>
            <begin position="288"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="163"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="405"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="551"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="609"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="693"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="810"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="174"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="217"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="405"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="426"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="551"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="617"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="699"/>
            <end position="709"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ82795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="852" mass="95006" checksum="AFBA0A29448D76D3" modified="2017-01-18" version="1">MSVSSSPGTDTPYEIDKLPRATLEEKITILEFFHSNESELTRGRLVNLFNKKICISNSSFSEWLKREEELKVKYKKLVDTTQANGASINSPQSILNNLLAINTTNDGIGYPTHSNNTLGFHRNSTDGNFYNNNNNSNNNAATAAAISQSHRNSIISNDYRSVSNEHHSRGHTPVPTNSQSPNKYQTDPMQSMQPNRTSTGTSTPTGLNMTSRNSTLNNQDNTTSTTITTTTNDLNNNTSTTTTTNNNNNNNNNNNNNNNTNNNNDSMNNTNSGLTNLNKKHGKSLSLAVKKPHKSKYDKMNKLMDKMVIKRKMSNLPITEAILKEYWAKYAKKCDITDLKRAEAPSNGWLDHFKNRHNLKAGLINMHNNPHQQNHPSLTNNPPSMMKNFTQLGDMSIRNFQQSAQIAHQEQTTGNTKYGENKVETNDNTENGPASNEPQNGPMNVMSALMEPSSSNIENNILQNQNNTEKTNTFLPPVTTTNEMLETPNTNNVLHRNNSTNFLMSSKLFPSTSDILQYNNINTQKSTDSFYKIFGHNLMNNHLNTLSTVPVLNNNNNNNNMMQNGQGPNGLPSAQYSSTTLEGINSVNAFIREQQQQQQQQQQQQQQQQQQQQQQQGPQGQLNLITNNPSTLNPANAPFSSNATSKRNSISFGEANPFVKAERTSLSNQQQPVPNGQANTVFNAYGRVQFGNHAGGITGDDEDKEEDDVYSNLTSNSSPGIKLLSNLIEGNSDINDNDILQINVPRDFSYEEVERTLSVHCLRYLRKNLQSYPASYKALKILIETFKNETTQNTDKSLFEYLNSIKTSHQQPALENNGIATTTNKKGKGKKTANVDDSNTNQEENSDGRGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7F2</accession>
    <name>A0A1E5R7F2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Vacuolar membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3433</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7F2">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7F2"/>
    <dbReference type="InParanoid" id="A0A1E5R7F2"/>
    <dbReference type="OrthoDB" id="431202at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022127">
        <property type="entry name" value="STIMATE/YPL162C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31735">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEIN YPL162C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31735:SF1">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEIN YPL162C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12400">
        <property type="entry name" value="STIMATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="156"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="unsure residue" description="I or L" evidence="3">
        <location>
            <position position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ82824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="334" mass="38450" checksum="62D80C8C9D0D3700" modified="2017-01-18" version="1">MRLVKFLLKKLAKVYKAERKHKISILPMLLARQSLLDXQKNKNPPPNEECLLLGPLSLTIQGFMGLIILSTLLIKRSKEHPRRKMKVWMYDISKQILGSLSTHSLNVVISILKGSDEDTWLDASSLLRRSSGLSWAKNTGQGSNDSDNNNNDDQCYWYFLNLFSDCTIGIPILWAILSLSNYILIKKLKIQNLNSGNYYDARTKHGPLASAFFKQFLVFLNGIIWMKFCIFVILLYFENVMEFFANLVLGWTSKIKHLEIVLVMFVIPILLNCFQYYCVDNIIKIDTHGEPSSSPVYKDNYYLNFEQPGTSXEDEQDQEQQQSLGNNRSVYNSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7F6</accession>
    <name>A0A1E5R7F6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Chromatin-remodeling complexes subunit NGG1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g3139</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NGG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7F6">
        <property type="interactions" value="429"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7F6"/>
    <dbReference type="InParanoid" id="A0A1E5R7F6"/>
    <dbReference type="OrthoDB" id="3972607at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019340">
        <property type="entry name" value="Histone_AcTrfase_su3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13556:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13556">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10198">
        <property type="entry name" value="Ada3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="123"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="607"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="698"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="123"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="139"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="171"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="195"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="618"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="719"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="733"/>
            <end position="744"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ82811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="744" mass="83323" checksum="0BF2D2F630022082" modified="2017-01-18" version="1">MTSNGTSDADSVSGNLEPQGILASMLKTLNLSFDQDMGMLNGEGVRNIPKDLKKLYQFRHLISDLVESLDDSLAPQPPVTSSAAQNEKHSLEFSLSNTKDNFPVATKDESAAWLAESTASVSTVDSNQSTVNGDLNNVQDEKARTESKKRTASEKYLAASENRKASLTSGSKDDFMPIDDRTALENGHSNTSNVVEEEEEDDEDEIPISKRRKFIIDKDKMENDPQVKNPKSEFVLSQTLPKAAALLGLFDEKRGLEATGEDYFKQKYAVASYPTQDLTELLPGRMPDLDLSNPKPTNQIQFTTWLSYVDPFFRSFTEKDQDFLKEKHVIPASILKKSNKHAITATTKTNNEIEKKDTKIIEQNSEVYDPEVTPFLIPKLGPLYSDVWLKEDNNKNIANITLPSIPAPTSILPKGSSSLLNDNILDIDNRISCGPLASRLVSAILKEEEGEIIERAEANDEESAIKQEEQSAISSTATTPFEENDLVKQATIPFEWKLDNMEVNQKLEYLSFEEKLKQELRYVGIYMNDEEEGQEFDWVYGREDDEISAELRQLQDKLRVVQKKNYERKQVLKPIIEKHLAWQEYQHILDDLDKQVDQVYVKRVRAPKSKKKKHXPVFTSNASSPSIDGVAGTSNGDHLNSTSQAAIQAAHQQAANQSLRGLIDKRKKWISNIGPLFGTNPELMKRMPKETIFQEIADDEGSGVSRKGTKIHRAGTGAEEVEGEEGEEEDVFGNSSNSKNIIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7H9</accession>
    <name>A0A1E5R7H9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">mRNA export factor GLE1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Nuclear pore protein GLE1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Nucleoporin GLE1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">RNA export factor GLE1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AWRI3579_g3213</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Nucleoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GLE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7H9">
        <property type="interactions" value="131"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7H9"/>
    <dbReference type="InParanoid" id="A0A1E5R7H9"/>
    <dbReference type="OrthoDB" id="3972065at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044614">
        <property type="term" value="C:nuclear pore cytoplasmic filaments"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.510:FF:000003">
        <property type="entry name" value="Nucleoporin GLE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.510">
        <property type="entry name" value="GLE1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012476">
        <property type="entry name" value="GLE1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038506">
        <property type="entry name" value="GLE1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12960">
        <property type="entry name" value="GLE-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12960:SF0">
        <property type="entry name" value="MRNA EXPORT FACTOR GLE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07817">
        <property type="entry name" value="GLE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="10" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="178"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="225"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00026227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00075092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00075681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OEJ82870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="550" mass="63184" checksum="075F266580F5C96B" modified="2017-01-18" version="1">MMRISFQDLINEPSDDEDNLYEPGXESPNANMLNIKHSMXXSNTLDVAVDEELSNFLATSLRLDSKNIPKYDRSHXKYNNISNKERSTATLNSRNSLNSPKPFLFEELFEPXSXENGGNSGLLQLQSSKKPLSHEKKIPLLKNLLDNMQQIKQNNALALEKVQLEKQKQIEEEKRRAQEAKRVEEERRKIEEEERKRVEEEKQKKAKEAELKREQMRNELLARQKAEAENQKKKQELDKQKQEELKKEELKSSVGVYSPSSIEAEFEKHKQRIKDIKEQIVIPASNDKELKGTLSKHKRKINPKFGQLTNSFQQLQSVTKELIGLIDQTKTSEMAYKWILNFIAKAVVSQAESEVGVKPEAAVSLGKLTLSLMCTYPELLPDFLLPRFVKKCPLVIGYSCPLDTEQQRTXMGWKRKSDGMWEDEVKYNERLSGMVTLYSTISRLVVPNVEQMIHPLPMAYSWRMVSRIANTNKELITNTHFAVLGAFWESCAHFFLLCYGNQAAKLLTLVTMDLTNLVSDKKLVSAARLRILAETWQNSGTISTFPELDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7N1</accession>
    <name>A0A1E5R7N1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">H/ACA ribonucleoprotein complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Nucleolar protein family A member 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g3221</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Common component of the spliceosome and rRNA processing machinery.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Required for ribosome biogenesis. Part of a complex which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ('psi') residues may serve to stabilize the conformation of rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
            <location evidence="1 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7N1"/>
    <dbReference type="FunCoup" id="A0A1E5R7N1">
        <property type="interactions" value="636"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7N1"/>
    <dbReference type="InParanoid" id="A0A1E5R7N1"/>
    <dbReference type="OrthoDB" id="5364946at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034513">
        <property type="term" value="F:box H/ACA snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031118">
        <property type="term" value="P:rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000015">
        <property type="entry name" value="H/ACA ribonucleoprotein complex subunit NHP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002415">
        <property type="entry name" value="H/ACA_rnp_Nhp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00883">
        <property type="entry name" value="NUCLEARHMG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="10">
        <location>
            <begin position="42"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ82907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="155" mass="16961" checksum="A560E39217738871" modified="2017-01-18" version="1">MGKDKTTKESKSEASSEDLYESRLPAVVKFAEPLASKKLNKKILKTVKKASKAKNVKRGVKETVKALRKGEKGLVIIAGDISPPDVISHLPVLCEDQHVPFVFIPSKQELGSAGSTKRPTSVVFIVPGSNKKGDKKKEEEYKSGFEEIVKEVKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7R0</accession>
    <name>A0A1E5R7R0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hsp90 co-chaperone AHA1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g3182</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5R7R0"/>
    <dbReference type="FunCoup" id="A0A1E5R7R0">
        <property type="interactions" value="1350"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R7R0"/>
    <dbReference type="InParanoid" id="A0A1E5R7R0"/>
    <dbReference type="OrthoDB" id="567237at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08892">
        <property type="entry name" value="SRPBCC_Aha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.20">
        <property type="entry name" value="Activator of Hsp90 ATPase Aha1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036338">
        <property type="entry name" value="Aha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015310">
        <property type="entry name" value="AHSA1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013538">
        <property type="entry name" value="ASHA1/2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 HSP90 CO-CHAPERONE AHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009:SF22">
        <property type="entry name" value="LD43819P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09229">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01000">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103111">
        <property type="entry name" value="Activator of Hsp90 ATPase, Aha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Activator of Hsp90 ATPase AHSA1-like N-terminal" evidence="3">
        <location>
            <begin position="13"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="179"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ82936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="359" mass="40743" checksum="1E07856668201A30" modified="2017-01-18" version="1">MVVQNPNNWHWVDKNCIYWAKEYFEKKLPALDSTKQGSNIHVVKLSNFDGDCEVNQRKGKIISLYDLKMTVLYEGVDASSGEKYEGSITIPEVAFDTEVDDYQFEIVVYKETVKVGEQFRKEIRSDLIPQMKAVFQQFGKDLLAANAEDIQIPESEVSSVFTKQNLSSSSTVLKSAPKTSSAASANSTTSSNDKPKVTVPLSTLGAQKISTYNSTSLHLEPTFNCRSRDLYETFLDKGRVNVWTRGSLQVVKSTDMGSPKLLENDEFKLFGGNIDCIITALDSSDVNEKIVMKWRMNDWLEGYFSEITLNFKQSDEYNETKIQIIWDKVPVGQEDRCKKNFEEYYIKSIKLTFGFGAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7V5</accession>
    <name>A0A1E5R7V5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AWRI3579_g3409</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R7V5">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R7V5"/>
    <dbReference type="OrthoDB" id="15433at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="entry name" value="4'-phosphopantetheinyl transferase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="4'-phosphopantetheinyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OEJ82977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="124" mass="14056" checksum="88AB001BC00AB039" modified="2017-01-18" version="1">MHELQQKEVAQTSIADVNTIPLHIRYAAGVWSVKESVYKSLDLDEKPIAXTGKSQKVPTLFLFSSIFYKSNNTSGKPLINVDLHNLKAKYVPXRLQSQILDSKFHLSISHDKDYVVSHVVREVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R7W2</accession>
    <name>A0A1E5R7W2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SAGA-associated factor 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g3249</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Functions as component of the transcription regulatory histone acetylation (HAT) complex SAGA. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction and promoter selectivity, interaction with transcription activators, and chromatin modification through histone acetylation and deubiquitination. SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). Involved in transcriptional regulation of a subset of SAGA-regulated genes. Within the SAGA complex, participates in a subcomplex, that specifically deubiquitinates histones H2B.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the 1.8 MDa SAGA transcription coactivator-HAT complex. SAGA is built of 5 distinct domains with specialized functions. Within the SAGA complex, SUS1, SGF11, SGF73 and UBP8 form an additional subcomplex of SAGA called the DUB module (deubiquitination module). Interacts directly with SGF73, SUS1 and UBP8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the SGF11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5R7W2"/>
    <dbReference type="OrthoDB" id="21557at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070461">
        <property type="term" value="C:SAGA-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013246">
        <property type="entry name" value="SAGA_su_Sgf11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08209">
        <property type="entry name" value="Sgf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="98"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU261113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ82961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="196" mass="21203" checksum="45D021FE617FC999" modified="2017-01-18" version="1">MVTTESLSQYYLENLLHNLAQQVISETVCGYQQNEAVLKQYHASNDXFAEDKRAQNTGEVSSPTPDPAAKKIPTSFIPSTSQTLAGLNNKELLSYIDNQDNNNKATGDGSSNKNGANDKNINNVTGAMAMALSDTFDHSASATPGPANLGSSITSAAVNTLKDEVVYLNCENCKRTIATNRFAQHLERCLSSRTRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5R8Q8</accession>
    <name>A0A1E5R8Q8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Methylenetetrahydrofolate reductase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g3229</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methylenetetrahydrofolate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000009">
        <property type="protein sequence ID" value="OEJ82913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5R8Q8">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5R8Q8"/>
    <dbReference type="InParanoid" id="A0A1E5R8Q8"/>
    <dbReference type="OrthoDB" id="16284at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004489">
        <property type="term" value="F:methylenetetrahydrofolate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00537">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003171">
        <property type="entry name" value="Mehydrof_redctse-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053806">
        <property type="entry name" value="MTHFR_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45754">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45754:SF1">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02219">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21895">
        <property type="entry name" value="MTHFR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="FAD-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MTHFR SAM-binding regulatory" evidence="9">
        <location>
            <begin position="410"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="137"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="387"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="141"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ82913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="687" mass="77369" checksum="3057B7EFD24C2484" modified="2017-01-18" version="1">MAPVTIKERYAYRAKHYTSPSVSLEFFPPKTETGKGNLINRIRRMTTAFNPMFLTVTWGAGGTTSEKTLELAALAQNAFRIPVCMHLTCTNTDQEIIDDALQRAKKAGIRNILALRGDPVVEDNEFMKLDNAYTENTPAFSTNSNLNNNNSNTRERSGSPAPNSKRFRYAVDLVRYIKEQHGXYFCIGVAAYPEGHCDNEDLEKQDVMRDLPFLKEKVDAGADFIITQMFYDVEKFLEFEQLVRTTISSDIPIIPGLMPINNFLTFNRATKLSHASIPQHIIDRFPVELRNDDDIVKSIGVDIIVEIAETIYARTQGRVNCFHIYTLNLEKSTAQIVSKSALLSTILEEDSSSEEENGEGYDSDVVIVDDEENDELIELTSVDADKKRKMHKRRRESSSTSYDGPSFMVGAHGNGSQQPSRKVLVSISQGNGSVGRDATWDEFTNGRFGDSRSPAYGEIDGYGPSLKVSPSTAYQIWGYPLAEQDLINIFTNYLLGSIPSLPWSDAGLSPETALIQEQLIQLNENGLLTLSSQPSTNGSSSTDKIFGWGPAGGFVYQKSFVEFFVDRNKWETQIKPRLDSFENPQVISYYMGGSNGDFQTNMQPNSSSVVTWGVFPNSEIQQTTIVEEESFKAWRDEAFSIWMEWSKLFPRNSEQHMFLKNTYHEFCLVAIVYHDFTNFDGLWDLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RA50</accession>
    <name>A0A1E5RA50_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein transport protein YOS1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2868</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the YOS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RA50"/>
    <dbReference type="FunCoup" id="A0A1E5RA50">
        <property type="interactions" value="247"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RA50"/>
    <dbReference type="InParanoid" id="A0A1E5RA50"/>
    <dbReference type="OrthoDB" id="15356at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013880">
        <property type="entry name" value="Yos1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15858">
        <property type="entry name" value="IMMEDIATE EARLY RESPONSE 3-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15858:SF0">
        <property type="entry name" value="IMMEDIATE EARLY RESPONSE 3-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08571">
        <property type="entry name" value="Yos1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184669" evidence="8">
        <location>
            <begin position="24"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ83751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="89" mass="9863" checksum="D77D9C691EEE158B" modified="2017-01-18" version="1">MIFGFGKLFYVILLLINAIAVLSEERFLRRIGLSGQHQQNTSSFTYGTQPQDASVKSKLITLISAVQTLLRIPLIGINVLVIVYELVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAA0</accession>
    <name>A0A1E5RAA0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ADP-ribosylation factor GTPase-activating protein GLO3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g3026</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAA0">
        <property type="interactions" value="724"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAA0"/>
    <dbReference type="InParanoid" id="A0A1E5RAA0"/>
    <dbReference type="OrthoDB" id="983479at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048205">
        <property type="term" value="P:COPI coating of Golgi vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08831">
        <property type="entry name" value="ArfGap_ArfGap2_3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45686:SF4">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE ACTIVATING PROTEIN 3, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45686">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE ACTIVATING PROTEIN 3, ISOFORM H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="7">
        <location>
            <begin position="11"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="145"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="457"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="147"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="206"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="267"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="323"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="470"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ83828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="526" mass="58293" checksum="267A57D9E45B697B" modified="2017-01-18" version="1">MTSNAPKAVTEQLFDSLQSNHSENRVCFDCGAKNPTWTSVPYGVFLCINCSAVHRNLGTHITFVQSATLDTWSTDNLRRFKFAGNHKAIEYFNKHGGAQILHSNDSRAKYTSPVAKKWKQHLDHCVDQDKLKYPNEVNLEDIEEGLADSSSSSNNNSADDFFDNWGNNNNKSGSKTSLKNLSSKASSPLTGTAXKARSPTAPTRISQQTTQSFSSSSPLTSSSSSANDKVSSSSAGDKASSILNKKTSRRTVLGKDSATGANKKHSILSSSRRPATVTKSSQLAQMNKPVSEERESFIDSFEREQARDGSRRSGVKVVESHIESPNYSASNSPNREHKKFSKQTSFFDEDDEDNNKNVQKXDVVEKELKPKFARLGFGMVTNNANELAEEQKLATQNKGFKYTGKVANKFGGQKGISSDEFFARGNYDEDTNKEAKQKLQTDFSNATSISSDAYFGRDQGADEEDEDGEFTGLRANTNNNNNRNNRNNSFANRFMEDQDDEFQVLREAVEQGATKLGNYLRDYMRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAD8</accession>
    <name>A0A1E5RAD8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RAD8">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RAD8"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="158" mass="18511" checksum="1C2E8016B08F4563" modified="2017-01-18" version="1">MDPKDFLSILSQTMTHITTALAFILPKLSLFYMQFPTITTVLMACXTVWGIYKVFIRHFTYVVRWSTRFLVALTAIYVYNRGVHDFYYTDLPFLKELFTEHHNEFFDLFRSCLRFAWSTVPVSQRIIIDKSMKSMGITVDSVYDEIKNALKTAVHATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAP7</accession>
    <name>A0A1E5RAP7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2909</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ83976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAP7"/>
    <dbReference type="InParanoid" id="A0A1E5RAP7"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ83976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="94" mass="10559" checksum="3FCF542F96038E99" modified="2017-01-18" version="1">MLTKTALPKLAKSSYISASSLMRSSCASVQNTRMYSPNSQTNPTDELAQKEKGEEKYYVSKREKEFLTKLNKYKEAHGGDLHKALEALSKEGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAQ9</accession>
    <name>A0A1E5RAQ9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">D-arabinose dehydrogenase [NAD(P)+] heavy chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g2597</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAQ9"/>
    <dbReference type="InParanoid" id="A0A1E5RAQ9"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="5">
        <location>
            <begin position="25"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="4">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ84000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="307" mass="34447" checksum="FB50AD6F76B45907" modified="2017-01-18" version="1">MGEKLLATKVDYKLNNGNSMPAFGLGTAYPPKPEDMHLVKESVIAAVKAGYRMIDTAYLYGTQKAIGEALQELYKXGVVKREDLFITTKVWPNFWNKVDDSINTSLKDLQTDYVDLALQHWPLCYPMITDNETGYLVMTPKNEKGQTLTVKDADYLTTYRQLEKIYLDETDKRIRNIGVSNFTSQCVEDLLNDKSIKTVPAVNQIELNPENPQLEVLELCKSKGIAVTAYSPYGSIGGPILKTPLVKKLAEKFDISVNDVLISYLLTKGIIAIPRSWKPERAGSTIYYKILPADVIDELDGMNKIPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAZ0</accession>
    <name>A0A1E5RAZ0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Lactose permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2852</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RAZ0"/>
    <dbReference type="STRING" id="56408.A0A1E5RAZ0"/>
    <dbReference type="InParanoid" id="A0A1E5RAZ0"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000134">
        <property type="entry name" value="MFS sugar transporter protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF24">
        <property type="entry name" value="HEXOSE TRANSPORTER PROTEIN (AFU_ORTHOLOGUE AFUA_8G04480)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="193"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="355"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="393"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="423"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="464"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="495"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="522"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="90"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ84065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="591" mass="65917" checksum="9B6894518D672ED2" modified="2017-01-18" version="1">MLGFDLKKHDESVGDTPLSSMSIHNEETSAKKEYGLFETPGASSTSLEPAFDSLSEHPYHGLEVTIDSVLPSYGGKKWYQLHHIQQLCWVLFIVSLTASNNGYDGSLLNSLYTMPDFNNAIGNVSGSIMGALSNGYVFGLLISFLASARINDAMGRKYALIIGNAIMIGGVIIQSCSGTWAKNLPEHYEKRDIMGMLTAGRIVLGIGSGIIQVAAPSLISELSFPTHREVVTTMYNASWYLGAIVAAWVSFGTKGLKNHWNWRIPVILQCMFAVIQLILVPFFVPESPRWLISKNRMDEAKNVLDKYHGGGYEGAETLIDYEMTEIQLAIEKERAANATSSYRDFIKTKANMKRLWIFTWVGIFVQLSGNGLVSYYLGKVLTSIGYTQTSEQLIINGCLMIFNYGSCIIQSVGIVPYIKRRQVFGMSFTGMIFCYVIWTVLSAINQERHFEDKGLGKGVLAMIFLYYFFYNLGLNGMSITYVTEILPFTLRAKGFNLYLTVLNLGSIFSGFVNPVAMDAIDWKYYIVYDCILVVECCVYYFTFVETSGRTLEEVAEVFGDGIEDLSKVSGIAALEDGKLKPDYEVEHEEYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RAZ7</accession>
    <name>A0A1E5RAZ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2968</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RAZ7"/>
    <dbReference type="InParanoid" id="A0A1E5RAZ7"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="343"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="452"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="599"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="682"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="803"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="218"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="243"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="361"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="462"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="478"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="599"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="625"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="687"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="705"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="803"/>
            <end position="824"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="844" mass="93216" checksum="29D90BDAD31DE5CF" modified="2017-01-18" version="1">MSSSNTQNNNPISITKGYLSPTLSPSFFPLNVGDDSSMPFMSLDKSTNNTHNMNGSSAMVTTSLSKLGDITRGRGRQRSLSNSSFTGGETPLSMLTKRTSSVSPIRFPSSTTSTTTTNARSTAPRMFNPEYFKTHNVNATSNSAATLSSLLANHNASNNAATTSAHHPTQSTANFNSIAQFLSSSLIGNQAPVEKKKKKKIGTKQKKAKQEETTPLFAVPSVPSGAASSASASTSKRQMTPVSCAISETTSSSSNIISDANELRITRSNNASQRKTSHKANQIATREIPLENENFASRHEEFASGNEDFVSGHKDKDSMIEKFLDQGQKHPFITGNIAEDVVRGAGSSSRHMKKKNSVHSEDDEDEEEEEEXXDDDDIPIDFVEMVEQSGLLDNTIDDASPQELMSHAKRLRNTKNVHKHMKRYSHISAYSSVTTAVPAQAANQWEASMNERHQNGATRPFSGHDGRLPYDQDDGRNRSSSPVKSQQEVTSETQLASYSSSQYEMAERQLRELQQQQVDLKMQQLQLQIEELRLTNDRLRYAIDEKKQYSTSNNGSASQSNMSLGQGSMNEYEMGQRVKLLESKIGDYEAVISQLTRASSMGRDGSSSNAQQHQISNHEHHSSEEPWTNYESTPYSPQTSNHNNNALRDANFDEAGSCTMSNCDSQLHVKKLRLSKIDSTQLEQLTRSRTNSRSTSETGSPLKAGSKERENRRHSKNIEHLEDYPFTTRNITGEDDHELKLDEGTLNLEDSAAPQNNVNVLKDISNTTSTAQPQSAPMSTMSSSHSDTQMSQWKQSMQTGSYQHKHSTSISMESTCSNDTSVSRHSNKRSRAGYNLNLSIELKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB15</accession>
    <name>A0A1E5RB15_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">EH domain-containing and endocytosis protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g3085</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB15">
        <property type="interactions" value="356"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RB15"/>
    <dbReference type="InParanoid" id="A0A1E5RB15"/>
    <dbReference type="OrthoDB" id="524326at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd14285">
        <property type="entry name" value="UBA_scEDE1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF170">
        <property type="entry name" value="DYNAMIN ASSOCIATED PROTEIN 160, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EH" evidence="4">
        <location>
            <begin position="12"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="4">
        <location>
            <begin position="145"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="4">
        <location>
            <begin position="286"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="3">
        <location>
            <begin position="1240"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="233"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="383"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="566"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="937"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="599"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="753"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="844"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="383"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="424"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="455"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="476"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="569"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="943"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1039"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1063"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1090"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1101"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1122"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1160"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1180"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="5">
        <location>
            <position position="897"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ84089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1282" mass="139400" checksum="2E6C3AD075264F01" modified="2017-01-18" version="1">MSQFKVPLSAEETGVYQNKFNELDPEGLSIVTGESVRPLFAKTGLPNDLLAEIWGLGDFDNTGFLTFQQFAICCRLIGYCQSKGGNTNGVALSPTNYEIFTRLPQFGNANYATSSPRPQSPMVQRQTSSNSFANNQSIPVPSSMDIAKYSQLFDRSSNGQPVLNGDTARDIFMKAKLPTPTLGAIWELCDQNQVGSLDKPQFTMAMYLIQLFMSNAITSLPPTLPTSLWQAVQXPQTPQPQPQKPSSPYMRXVSNQNTGNSLDRQASLVKRTFNTSSSNWTLSADKKKNFDAIFDSLDKSKEGKLGSGVLVPFFLTSKLSQDVLATIWDLADIQNNAEFTKMEFAIAMFLIQKKNAGENLPDIIPEELLNSPALGLNTNPQVQAPIQQQQQQQQQQQQPLQSDPVIPNRNSKPTFDQPPPVASVAQQNNTGTNSPLDDLLGLDNSSFSPAPPAPHAANNPVSVASTGNSANNASSPLLTSSNTGLRKFKPSSTFGQSIIKEEPEQEQNSAQAINPAVVGAAGAAGAAVGAVAGVTAGAAAASSNAASRPAYQSSLPNVPNFANIGLPSMSGSPTATQHAASTSRSVNPSMDNADSGFRLSQATTDLANLSNQVRSMSNQSSILNDKKARNEQELQRVNATKANIEAKLATLRASYQQETQQSERVEXLLAQSKQEVNTLTEQLNVAEANHHAAELKLQEVQSQLVEIENSNSQIKEKIGGFNSMTQSLQADLESKQTELQQKHSMLGIGEKQLEVSQLNAANLQKEIAELESHFDVFVNKKKELDDYSTNIEKQHAMLGEKHQAFVDKSKELETKTSELDQREEDIKTRTTQIEEQEKLYHEHIAQLQNMFEHLNLEKQKLDSANADLENQQYEYASRVQELAEKQMQLAMGHMPSDEEITXLQNTVADKKLNGQTTKKTVITEDFSAPEVATPXRTVASVTEKSEDVFDDTKSATEATSINDRDVSNTXILPDDTNLEDVPKHTSTTSSTLNNPPQSETLDHDNLNDEIPGHWGAGKPVGAVAGQNEEEDEQLADDNASFNSIQKSPKLNQSESLPAAAGANETTVTTGQPANDAHEAKAVSPSNSPNIHEEFPPIRELEQDESDSSDSSDDDEEFRDAIPSNQELPQNFTSASAPAKKEEDDDFKDFDDLQQAKVDEGFDDDDDDEENEIPTGTVEDSFTSSTIPAPSAPASSSVAENSEWNQLFSGLEKPIAHXLPQTPASPPATPLKQTALPMKIATTPKSLAVEELCSMGFSEAEAKRALESCNWDLDTATNFLLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RB61</accession>
    <name>A0A1E5RB61_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rad61 Wapl domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g3094</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RB61">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5RB61"/>
    <dbReference type="OrthoDB" id="4069585at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.60">
        <property type="entry name" value="Rad61, Wapl domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031550">
        <property type="entry name" value="Rad61_Wapl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038496">
        <property type="entry name" value="Rad61_Wapl_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16997">
        <property type="entry name" value="Wap1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad61 Wapl" evidence="2">
        <location>
            <begin position="256"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Rad61 Wapl" evidence="2">
        <location>
            <begin position="429"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="580" mass="66294" checksum="7A0057263D119F91" modified="2017-01-18" version="1">MKKAYGRHSHKTHKKVDLKLLLEQPTNKQSSKQAEGIEFSDSDTDAIDKSPALYHDDSPTANNTETSFLSSAFNSMSSDPGNKALELKSGVPDAKKTSDPLNGLLDFLTVPKCKRKSKRIKPNPFHHSEQFSENNGFDFKEALTDVNTFVESLQQPDEFNDSENKVQKEAQIPGQVTKCLIFNDESQLKKNVYGSYRSMLRSSVEYVVDDDDXDKLSRQNXEVKITPIQDDVGSLAKEDESSADEAXETNENVPLTQNFNQLKAMGSNIQYNDDIRFVMEGSLPAVKKFITMIQEVRSSEGLRNYIANYYTDEFWDLLQNNADDPYLLMVLSTLGRMHSKLAIGHFXQLSRYTPRNDLGKHYQTQYEEIFKVFGNHTTLGVKTLNXLKEKKQAIIEDPRITDYLARIPFCPEIISLLPFVDLDKNAKSFWKLTTNINIQNNIENADYIKSCVSLTNTRNFDTRNVTNLLNFSLQYILTNFQDTDKQHLLILHLGLCSNILHLHKKKVLKVSYDLSPIYENLDMAKLDFVHNLFLLNYAYLLKFAGKEIDTSITKSLLCWATNIDDLNNSFKERADYILNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBB0</accession>
    <name>A0A1E5RBB0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Holocytochrome c-type synthase</fullName>
            <ecNumber evidence="10">4.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2487</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Lyase that catalyzes the covalent linking of the heme group to the cytochrome C apoprotein to produce the mature functional cytochrome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>holo-[cytochrome c] = apo-[cytochrome c] + heme b</text>
            <dbReference type="Rhea" id="RHEA:22648"/>
            <dbReference type="Rhea" id="RHEA-COMP:10725"/>
            <dbReference type="Rhea" id="RHEA-COMP:10726"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="ChEBI" id="CHEBI:83739"/>
            <dbReference type="EC" id="4.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the cytochrome c-type heme lyase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.17" evidence="10"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBB0">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBB0"/>
    <dbReference type="InParanoid" id="A0A1E5RBB0"/>
    <dbReference type="OrthoDB" id="4243at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004408">
        <property type="term" value="F:holocytochrome-c synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000511">
        <property type="entry name" value="Holocyt_c/c1_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12743">
        <property type="entry name" value="CYTOCHROME C1 HEME LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12743:SF0">
        <property type="entry name" value="HOLOCYTOCHROME C-TYPE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01265">
        <property type="entry name" value="Cyto_heme_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00821">
        <property type="entry name" value="CYTO_HEME_LYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00822">
        <property type="entry name" value="CYTO_HEME_LYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0349">Heme</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="9 10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="48"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ84184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="261" mass="29646" checksum="0F45DC9180FC7CF1" modified="2017-01-18" version="1">MSSSADSADDSKCPVPHDKRXTWLQSMKNSDTTNSSSSAAPVPANAESDDKCPVPHESRDIWLKSQGVTKSTASAPTQDIECDSSTLPETPNYKTDVKLPEEREVSSIPRTNSHGNWIYPSQKQFFEAMLRKNWSPDAKDMETIIPIHNSINERCWNYIKLWEKNQGGDECGGVQLTSFKGDSKKMTPRAFLRSYILGYTKPFDRHDWTVNRCGKNIDYVIDFYSEEDSKGRPQVYLDVRPKLNSFEGIKLRVMRSLGLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBG9</accession>
    <name>A0A1E5RBG9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2891</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2"/>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBG9">
        <property type="interactions" value="1117"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBG9"/>
    <dbReference type="InParanoid" id="A0A1E5RBG9"/>
    <dbReference type="OrthoDB" id="193499at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000025">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase CYP19-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000029">
        <property type="entry name" value="peptidyl-prolyl cis-trans isomerase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0697">Rotamase</keyword>
    <keyword evidence="4 7" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="8">
        <location>
            <begin position="7"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="314"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ84244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="377" mass="42062" checksum="F3308E6C802D07B1" modified="2017-01-18" version="1">MTHRKTYFDLSIGDQPAGRVVFELFDDETPRTCENFYQLCKGGIGNCTTKPDIPLSYKNSIFHRVIKDXMLQFGDFTNFDGTGGESIYGEKFEDENFIHKHTSPFLLSMANAGPNTNGSQCFITTSNAPLTHLNNKHVVFGKVIQGKRIVRAIENIECDQGDKPVKDVKITDCGVLPDDYEVPADAEVLQKDQYGDNYEPSIADEPRVDLGSFESAMAAVNAIKAIGTTCFKDKNFKAATEKYSKCRDYLTDYAKACNDKYATDENTNTIKSLKATIYNNIAISALKDGNYKEVLAAATQVLHIADLEDDSAKAKALYRRGLAYFHLKDPEMALTDLEFATMYQPNDKSIHKAIAEARALKKQQLAKQKKSLSKMFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBH0</accession>
    <name>A0A1E5RBH0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cytochrome oxidase assembly factor 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2893</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBH0"/>
    <dbReference type="FunCoup" id="A0A1E5RBH0">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBH0"/>
    <dbReference type="InParanoid" id="A0A1E5RBH0"/>
    <dbReference type="OrthoDB" id="5586401at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039870">
        <property type="entry name" value="Coa4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13639">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 4 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13639:SF2">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 4 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ84242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="90" mass="10736" checksum="84CD72C52EE79F43" modified="2017-01-18" version="1">MDSKYYQEALEDYKEQQQEQAQTKEEADLWDKRINKTGCYVENMALQLCHADTNDWRQCMKEMALFRKCWDQHGNNQRVGTVDLVDNKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBQ4</accession>
    <name>A0A1E5RBQ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cytochrome c mitochondrial import factor CYC2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2521</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBQ4">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBQ4"/>
    <dbReference type="InParanoid" id="A0A1E5RBQ4"/>
    <dbReference type="OrthoDB" id="432685at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001834">
        <property type="entry name" value="CBR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370:SF189">
        <property type="entry name" value="CYTOCHROME C MITOCHONDRIAL IMPORT FACTOR CYC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="7">
        <location>
            <begin position="41"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601834-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ84330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="349" mass="39837" checksum="72CC4B58304AF292" modified="2017-01-18" version="1">MLKGKHTKRILTLSIGIGSAWVGYSLYHRNYNQESNELSPERFAKYRISWRKDIDESHYLMELTPVVSQKKNLWREHGYNKLWSVEVKQPEIHVVRSYTPLPLVHSLSEDAKETKDQSFNLKVLEDGENGGGKLMFYIKQYANGEVARWLHGLPANSELELRGPFVEYELPEFPENEICRSRSFLNHKXALKPELNKENFCYQPFDINFFTGGTGIVTALQLMLTENPFRGQIHLYHGCSDIKELGPLLPLLSKLADAKRVHLDISXNARTEVEKQWKKLLDTPHPYQGIIPFSSATTQAIFRPILSLVVGPEGFIKAVSGEKFSMNQGPINGILGEKGWNNSNVFKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBR3</accession>
    <name>A0A1E5RBR3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ubiquitin-binding protein CUE5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2527</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RBR3">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBR3"/>
    <dbReference type="InParanoid" id="A0A1E5RBR3"/>
    <dbReference type="OrthoDB" id="9942608at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16461">
        <property type="entry name" value="TOLL-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16461:SF5">
        <property type="entry name" value="TOLL-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00546">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CUE" evidence="2">
        <location>
            <begin position="180"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="436"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="382"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="471"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="508"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="536"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="557"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="571"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="607"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="693" mass="76920" checksum="3B425EA55DD0685E" modified="2017-01-18" version="1">MSDVEATATSKSATQPIXEEPNPFDKMENTPKEKESNAXQDADEGEHAAQIDAATEDTKAESKESVDEENSELGKTESLAVVDKEDAKESETATDTKDALESSSKKTTLEEQLEDVSLDDDQLPAATKKEESSTEDTNDEPAPVLPPRRATTSSSSNEDQSTLSAENKNDSQKGNTPANTKHPLLAQLEEAFPQFEEKYIVAVAIASQGNVEAAYNALLYLADPSATEVDIPVNTAKVPPAQPAXSLSPSELAYQEQLKADELLARQLSERYNAPRKSNSRTSKGFSADAARKPRRVSRPTDPQGPPRPRRSLKEYNVSSNDDVDDDQYADRYFNHESDEEDGLTTFIEKDLPMLKDNVNKSLRETSSRVGNWIKSFSGGEQQQQQQQQQQQSXNDPRNSGYSSMNANDLDESYYNDDTDDRLFLQDSRYSNQANVYSDEYVRSSRGNNNFNDSKTRPGMNMRGEKKRFNSFGNSDQNLNHYDTDSGRNVFNRNDRNKNNTGRINIEYGDKKHFKNDEKHHDDMNEDYMPPPTLPPRRSTTSTSTSNATATAEGTTPIKSNEATIDTPFNDNKKFKTPAKMEQEKRAGVVQLNEFDAADDDDGETXLKNTPLSDSNSTRQKPSLSSLRVVDPEGASTIPWNNANRLGGSFSGGRDDKYSDLMNVNTPTKGKETPSVAKVSKNAQDEDDLDFSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RBU6</accession>
    <name>A0A1E5RBU6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA repair protein RAD51 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2989</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required both for recombination and for the repair of DNA damage caused by X-rays.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the RecA family. RAD51 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000008">
        <property type="protein sequence ID" value="OEJ84374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RBU6"/>
    <dbReference type="FunCoup" id="A0A1E5RBU6">
        <property type="interactions" value="825"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RBU6"/>
    <dbReference type="InParanoid" id="A0A1E5RBU6"/>
    <dbReference type="OrthoDB" id="10251254at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070192">
        <property type="term" value="P:chromosome organization involved in meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000730">
        <property type="term" value="P:DNA recombinase assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042148">
        <property type="term" value="P:DNA strand invasion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990426">
        <property type="term" value="P:mitotic recombination-dependent replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19513">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000008">
        <property type="entry name" value="DNA repair protein RAD51 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000092">
        <property type="entry name" value="DNA repair protein Rad51 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011941">
        <property type="entry name" value="DNA_recomb/repair_Rad51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016467">
        <property type="entry name" value="DNA_recomb/repair_RecA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010995">
        <property type="entry name" value="DNA_repair_Rad51/TF_NusA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020587">
        <property type="entry name" value="RecA_monomer-monomer_interface"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003301">
        <property type="entry name" value="PRK04301.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02239">
        <property type="entry name" value="recomb_RAD51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942:SF39">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD51 HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942">
        <property type="entry name" value="RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14520">
        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005856">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47794">
        <property type="entry name" value="Rad51 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50163">
        <property type="entry name" value="RECA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="9">
        <location>
            <begin position="135"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="RecA family profile 2" evidence="10">
        <location>
            <begin position="314"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ84374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="381" mass="41468" checksum="1E7B91B64B7F14F5" modified="2017-01-18" version="1">MSQVLSHSTQDSNQAMDIDQHHGDQSFEKNDQNLGVATPGEDEQQQVAAEEEEEAVVAFLPLERLQVNGISPGDLKKLRESGLHTVEAIAYAPKKDLMEVRGISEAKADKLLMEASRLVPMGFVTAADFHLRRTEMICLTTGSKNLDSLLGGGIETGSITEMYGEFRTGKSQLCHTLAVTCQIPLDMGGGEGKCLYIDTEGTFRPIRLISIAKRFGLDPEDALNNVAYARAYNADHQLKLLGAAAQMMSESRFSLIVVDSIMALYRTDFAGRGELSARQMHLAKFMRALQRLADQFGVAVVVTNQVVAQVDGSAGMFNPDPKKPVGGNIMAHSSTTRLGFRKGKGEQRICKVADSPCLPEADAIFAIYEDGIGDPIEEDKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDH2</accession>
    <name>A0A1E5RDH2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mating factor alpha-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g1930</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDH2"/>
    <dbReference type="FunCoup" id="A0A1E5RDH2">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDH2"/>
    <dbReference type="InParanoid" id="A0A1E5RDH2"/>
    <dbReference type="OrthoDB" id="3766782at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000772">
        <property type="term" value="F:mating pheromone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007618">
        <property type="term" value="P:mating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016326">
        <property type="entry name" value="Mating_factor_a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006742">
        <property type="entry name" value="Mating_factor_alpha_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008675">
        <property type="entry name" value="Mating_factor_alpha_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04648">
        <property type="entry name" value="MF_alpha"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05436">
        <property type="entry name" value="MF_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001866">
        <property type="entry name" value="Mating_factor_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184699" evidence="1">
        <location>
            <begin position="24"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Mating factor alpha precursor N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ84934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="187" mass="20216" checksum="94ED2B3D6DA9060D" modified="2017-01-18" version="1">MKLSTTIAATTAATTALVAGATAAPVSGASNSTSMEVPGEAVKYFLDLSDSPDMALVPINNGNTTGIMFVNTTVIDQAYAETTSLSRKREASANAWHWLKFDVGEPLYAKRDANADADASLWHWLKFDVGEPLYAKRDANADADASAWHWLKFDVGEPLYAKRDANADADASAWHWLKFDVGEPLYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDI7</accession>
    <name>A0A1E5RDI7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein HRI1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AWRI3579_g1794</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HRI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ84970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDI7"/>
    <dbReference type="STRING" id="56408.A0A1E5RDI7"/>
    <dbReference type="InParanoid" id="A0A1E5RDI7"/>
    <dbReference type="OrthoDB" id="4045395at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd11692">
        <property type="entry name" value="HRI1_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.320">
        <property type="entry name" value="Protein HRI1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031818">
        <property type="entry name" value="Hri1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038744">
        <property type="entry name" value="Hri1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043047">
        <property type="entry name" value="Hri1_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16815">
        <property type="entry name" value="HRI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OEJ84970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="218" mass="24579" checksum="5AF627B735155227" modified="2017-01-18" version="1">MCEPVFSTRVSIKWESDPKPHEPTSTWVFTAGNEEFVDTRINLASSTPDWLITGYEKALATKAGYESTIMFCHALDNLCKPGEMPGADVGHFKNIAGSSDRLEEGAMMNPDIGKNMEYQEVWRTLDPIHSTVSDLKPLGVSTKHVENQVWKLEDSKGAFIKIGEFSQGVAVNAKNEYQCIRLFKQEVVYQYGNESYRIFAPFITGHLKQSNWVKTFQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDP5</accession>
    <name>A0A1E5RDP5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Negative growth regulatory protein NGR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AWRI3579_g1786</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDP5">
        <property type="interactions" value="84"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDP5"/>
    <dbReference type="InParanoid" id="A0A1E5RDP5"/>
    <dbReference type="OrthoDB" id="446113at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050825">
        <property type="entry name" value="RBM42_RBP45_47-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640:SF10">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="262"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="484"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="144"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="340"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="447"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="692"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="341"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="447"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="692"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OEJ85014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="755" mass="81960" checksum="0AA42C3636969C11" modified="2017-01-18" version="1">MWGSTSNSQSSKPWKITNQSSGSATXALLGAHHRFHQEHVPRQIPEDTIFEEVGNTMPYPSATGQANVHEDVVPLPPSSTMQSSENGELPRTLWMGDLDPSFNERTIELLWGSLGKQVRVKLIRAKKNLLIPCSTASATHAGEQYTQGSAHQSGAYQDTSTPASNSANNPVSPKTASASININGTNFIDPNTTQLHHAGYCFVEFDSFKNAQHALTMNSKPIPNVYDEHIHRCTNSDAKRTFRLNWASGATLQSIIPATPEYSLFVGDLAPDCYEQDLLTLFQTKYASVKTVRVMTDPITGASRCFGFVRFQDFLERELAVLEMNGVWCKGRPLRVAYATPRSSSSSASGPSSGPSNSRPRSFSENARHQGHDWNVHETILAXTTSPINSYXLSPSSSVGGAGAQPAIFDRRSSESATSTGLSPPKSSIAHNVSNIVTISEMPLGTTTVQNDSVQRRASTGSSSFAHGMMNNNMNANTNTSTSTTVFIGGLNNKIDEFQLYSMFQPFGKIVQVKIPHNKGCGFVKFGQRRDAEAAIVGLQGFILKSTGLPIRLSWGRETNTNTGNGGLLGSFDGNVAMGHQQPQQFLQQSITSPVGSTSEFGFPATQEYGAAESGPHIYHQDVFNAPGPPPVCXKTKRSSSLSYPMGLSQSLNMQDYIPQSGFGNGTFSEEFSTAFPQAGLQPNLYAVQQAPPVSSQVQQQQQQQHMPNSPGMPGXSAFDQTQQNITSSNLSQFPAFADPMFGMKRKSIDHHEWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDP7</accession>
    <name>A0A1E5RDP7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="17">ISWI chromatin-remodeling complex ATPase ISW1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g2047</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDP7">
        <property type="interactions" value="1127"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDP7"/>
    <dbReference type="InParanoid" id="A0A1E5RDP7"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17997">
        <property type="entry name" value="DEXHc_SMARCA1_SMARCA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000082">
        <property type="entry name" value="ISWI chromatin remodeling complex ATPase ISW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000002">
        <property type="entry name" value="ISWI chromatin-remodeling complex ATPase CHR11 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1190">
        <property type="entry name" value="iswi atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.30">
        <property type="entry name" value="ISWI, HAND domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044754">
        <property type="entry name" value="Isw1/2_DEXHc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015194">
        <property type="entry name" value="ISWI_HAND-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036306">
        <property type="entry name" value="ISWI_HAND-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015195">
        <property type="entry name" value="SLIDE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF49">
        <property type="entry name" value="SWI_SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY A MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09110">
        <property type="entry name" value="HAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09111">
        <property type="entry name" value="SLIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101224">
        <property type="entry name" value="HAND domain of the nucleosome remodeling ATPase ISWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="172"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="478"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="16">
        <location>
            <begin position="860"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="915"/>
            <end position="945"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ85025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1062" mass="122995" checksum="3779D777075BF144" modified="2017-01-18" version="1">MEDYLQKIKPFHDGLPSANPETRQKRYLLDKELVKKYKPVGIHQVSMTVKRFEHLLEISHLFKHFLLNKLEQKDEKFVKVYEILKKNSPHLLSTHHTNGKEXPXIHRGRMSEKDEDKQLMNGEEKSTALDETLAQDEEANEIDEEEIQFTESPLYINGSLRPYQIQGLNWLISLHRNNLSGILADEMGLGKTLQTISFLGYIRYLEKKPGPFLVIAPKSTLNNWLREFNKWTPEVNAFVLQGDKEGRAKLVSEKLLKCDFDVVIASYEIVIKEKAHFRKLDWEYIVIDEAHRIKNEESLLSQVIREFTSRNRLLITGTPLQNNLHELWALLNFLLPDIFADSSTFDQWFGGSSDDSEVGDKEQEEKDSVVKQLHAILQPFLLRRVKSEVETSLLPKIELDVYAGMSAMQKQWYRKILERDIDAINGETGKKESKTRLLNIMMQLRKCCNHPYLFDGAEPGPPFTTDQHLIDNSAKLKILDKLLDRFKQQGSRVLIFSQMSRVLDILEDYCYFKNFGYCRIDGSTDHEDRIAAIDEYNAPGSEKFVFLLTTRAGGLGINLTSADIVVLYDSDWNPQADLQAMDRAHRIGQKKQVKVFRFVTDKSVEVKILEKAKQKLRLDQLVIQQGRTSIEKDKKMKGKNDGKDELLSXIQHGAKDLFEKNDKANDEDDXFDLEKLLQDSEQKTKELNAKYSKLGLDDLQKFSNQSGGDTYEWDGESFKKTNVTAKVIDPLSLLNGNGEGGRRDRERNANYSIDEYYRDVLNPKSAAKPAAQLRMAKPPYFPAHQFLPIKLKDLMEKERLYNAKLNGVSPTMDDVKLTFGDLSDDKEENKRKLEILKLSIDVAEPLSEEELQEKEDLKLQGFTNWTKNEYRKFLTASGRHGRTAIREITREIATNKTEDDVYEYSKAFWANITLVDNYERVLKNIEAEEDKLNKLAVQQEALRRKVSQYRDPLRQMKIKYVSASSTYSLEHDRFLITMMLKHGLDKSNLFDLIKFEIRKSPLFHTDFFFQNRSSTDINKRCQVLLGYIEKEMNTGISIDAKLKKRLSNEDAAFESDHKKIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDV4</accession>
    <name>A0A1E5RDV4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phenylalanine--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="3">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Phenylalanyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AWRI3579_g2148</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Is responsible for the charging of tRNA(Phe) with phenylalanine in mitochondrial translation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDV4">
        <property type="interactions" value="757"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDV4"/>
    <dbReference type="InParanoid" id="A0A1E5RDV4"/>
    <dbReference type="OrthoDB" id="4457at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00496">
        <property type="entry name" value="PheRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.380:FF:000002">
        <property type="entry name" value="phenylalanine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000053">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.380">
        <property type="entry name" value="Ferrodoxin-fold anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005121">
        <property type="entry name" value="Fdx_antiC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036690">
        <property type="entry name" value="Fdx_antiC-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004530">
        <property type="entry name" value="Phe-tRNA-synth_IIc_mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00469">
        <property type="entry name" value="pheS_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF41">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03147">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00896">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54991">
        <property type="entry name" value="Anticodon-binding domain of PheRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51447">
        <property type="entry name" value="FDX_ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="15">
        <location>
            <begin position="139"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="FDX-ACB" evidence="16">
        <location>
            <begin position="345"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00073229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OEJ85080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="438" mass="50908" checksum="8A8B7889F94E3D74" modified="2017-01-18" version="1">MSTQQARAGENADLVVNGKSYQKDAEQTNVPQAILDLTDRHLHLNPKHPLGVLRSLIENKLNSVDQSFRTYNEFKPVVTVFENFDSLGFPKDHPGRNKSDTYYINKNHLLRTHTSAHEMECFGNILQKKTHKPGYLITADVYRRDEIDKTHYPAFHQMEGARIWSKDPAGXTQLKKDIEVLNKELEKIKNFTVEDCPYVEATNPKQSYMSEEEVHLVSTHLKKTFELIVAEVFKKKLESEGKGNEEIKARWIEAYFPWTAPSWEIEVWWNGEWLELCGCGIVQEQVFVNAGMEKDSTIGWAFGLGLDRIAMLLFGIPDIRLFWTRDPRFHEQFQENTITTFKPYSKYPGTVRDVSFWKNPDLEIHENDLMEIVRSNAGDLVESVKLIDSFVHPKTKKHSLCYRINYQSMDRNITNDEINELQEVVRNTLVEXYGVKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDX5</accession>
    <name>A0A1E5RDX5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pheromone alpha factor receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RDX5">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDX5"/>
    <dbReference type="InParanoid" id="A0A1E5RDX5"/>
    <dbReference type="OrthoDB" id="5402633at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038038">
        <property type="term" value="C:G protein-coupled receptor homodimeric complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004932">
        <property type="term" value="F:mating-type factor pheromone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14939">
        <property type="entry name" value="7tmD_STE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.920">
        <property type="entry name" value="Pheromone alpha factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000366">
        <property type="entry name" value="GPCR_STE2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027458">
        <property type="entry name" value="STE2_TM1-TM2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28009">
        <property type="entry name" value="PHEROMONE ALPHA FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28009:SF1">
        <property type="entry name" value="PHEROMONE ALPHA FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02116">
        <property type="entry name" value="STE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00250">
        <property type="entry name" value="GPCRSTE2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0675">Receptor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="433" mass="48365" checksum="DE09C905E0683BF7" modified="2017-01-18" version="1">MTSGTLIPDAFDESFNPNNILLSYESAXSMETQISFGELQSFFNRKMNQSIIYGTRIGAAAVAILILXMVTKNKRSPIFIINQFTLLIIVLHSALFLKYLASPYSSAIYTFTYFPQLVHRSNIYVSGAVNMLEVVLIALVEISLVYQVYTIFKSSENRKMTVAWISLSSALGLATIGTYFATAVISMLNIYEKSYSTSDSKLFNVSVILLASSINVMTLLLSLKLFFAVRARRFLGLKQFDTFHILLIMSAQSLIIPSILYILSYALPAKDGTSNLAAIATLIVVLSLPLSSMWASSTNNSSQVNTFNPNAAATYTDNGSFYSQTIQSAIEARRSTRKNXWNPAVDLSPNSEKTAKESYSNTLKKFGSSVNNNFRDEDDFTIDDKEYWTTKNKQQPDTQVSLSDIEXLPEALRQLKNNNLDDDIVHYKVISKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RDY0</accession>
    <name>A0A1E5RDY0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein DOA1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g2453</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RDY0"/>
    <dbReference type="FunCoup" id="A0A1E5RDY0">
        <property type="interactions" value="1416"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RDY0"/>
    <dbReference type="InParanoid" id="A0A1E5RDY0"/>
    <dbReference type="OrthoDB" id="10265988at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010992">
        <property type="term" value="P:ubiquitin recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.870">
        <property type="entry name" value="PFU (PLAA family ubiquitin binding), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015155">
        <property type="entry name" value="PFU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038122">
        <property type="entry name" value="PFU_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013535">
        <property type="entry name" value="PUL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849:SF0">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09070">
        <property type="entry name" value="PFU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08324">
        <property type="entry name" value="PUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51394">
        <property type="entry name" value="PFU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51396">
        <property type="entry name" value="PUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="147"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="229"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="PFU" evidence="6">
        <location>
            <begin position="363"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="PUL" evidence="7">
        <location>
            <begin position="476"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ85076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="727" mass="81462" checksum="7FA7BBD55449CD25" modified="2017-01-18" version="1">MYQLSSSLAAHDQDVKDIVAIDETSFSSCSRDGSVKTWFREKSSGSSVTWDHIVVHQSTNKSYINCLSFIDGIVYDGGNNKLIQGFPYLSNIQENENEPVFSLSKHTANVCSLSSFYDASLGDNYIISCSWDSTAIVWKNNEFLFQLRGHQSSVWDAKIIDPTTFITCSADRTVKVWSKGTCVKTFENLHNDVIRAIDVDLENSIFATSSNDGFIYVCDLQTGAPKATLRGHDSFVYKVKFAQNQKLISCGEDRTFRVWDMKKLAEQQCIVLPATSIWSFDQLSNGDIVVGSSDGMIRVFTESVERRAAAEQLKAFAEEVSNSSISSQTIDEKNISPPEILSKPGKKDGQVVVVKSLQGNLEAHQFTNNTWVKVGDVVGGVGSDQKVEYEGKKYDYVFDVDIEDGKPPLKLPYNANDNVYSVAENFLSKYELPASYREEIVRFIIQNTGGVAMDSTGLTAQAQPTTTPAAPASTLKVLPFTDQYLTLANFKKTLLSKGIVKINVNEEIQIPEEQLMEFENALDNQASNVDFLYTEAEFIKHNWKMKLPAYDIMRIITPFLPSSSKLVPFVHDGVASDDLNINLMAIRCLVNCFQNEEWGIPFLEQVDIYEFVFKSVKSDIPETFKQCPQLLIAIATLLFNYSVLTVRKSNLEMVPVVADALNNKFGASPVFINNEEAAYRLLMAYGNLATVEPTLKQFAKSIKWLKQVKTRYDHIGRFIDVFEDLSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REA9</accession>
    <name>A0A1E5REA9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cell wall integrity sensor MID2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g2356</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REA9"/>
    <dbReference type="InParanoid" id="A0A1E5REA9"/>
    <dbReference type="OrthoDB" id="4070729at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007567">
        <property type="entry name" value="Mid2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04478">
        <property type="entry name" value="Mid2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009184712" evidence="3">
        <location>
            <begin position="30"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="310"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Mid2" evidence="4">
        <location>
            <begin position="265"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="374"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="123"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="201"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="447"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="498"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="517"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="536"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="574" mass="58931" checksum="3F397E90940D267A" modified="2017-01-18" version="1">MRNNQKKQLLIPTVIALLISSQFFNTALAQAIAATAAGNTVTDTNTNSNLTNSANAASFSSSTAXGGVTTDTGGFTSLPITSSNSNTDANTISSTTSSSSISSTTPISSSLTFDTFATASSAFSSSSTTDNTFATPNAETSATNSTXSSLSSTTXFSSSDSGFSTSTGGVPSDTGFNNATVASSSSSMSSSRTSSSLSLSSSSASSSASSSLSSSSRSSTTSSSHSSTTSSTSSSSSSFSSSVSATDSSTSSTSSNSITTKPIGTHTTVIDGQTIYSNIYTTITATASVLPGGKQNGGSGGGLSSKNKKIVIGCVVGIGVPLLIAFGILVYYFFIKSKKVNFIDSDGKVVTAVKANKFTKLWYKIFLGKNANDRYDPSSTPPGMEEMEKSNNEGYTHSLRNSGPENITNMNIDNDMFERDVYLDENGNPVPSQGSAAPNVGRAATAPTGSKGNNSATQRNIDGDIVPGRKSYHGQTRSSVGTPYPINDPFTSGALPLNTNDASNATSSANNNNSSTGDNRYSGSTSQSQQVQNDMSNKSSSNANSSNYHDPYANSDEDSGYYEGDNNGINARNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REC4</accession>
    <name>A0A1E5REC4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Protein SSO2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1858</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for vesicle fusion with the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the syntaxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5REC4"/>
    <dbReference type="FunCoup" id="A0A1E5REC4">
        <property type="interactions" value="598"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REC4"/>
    <dbReference type="InParanoid" id="A0A1E5REC4"/>
    <dbReference type="OrthoDB" id="10255013at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070300">
        <property type="term" value="F:phosphatidic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031321">
        <property type="term" value="P:ascospore-type prospore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
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    </dbReference>
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        <property type="term" value="P:vesicle fusion"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Syntaxin family protein"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Syntaxin"/>
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        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF307">
        <property type="entry name" value="PROTEIN SSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="239"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="122"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="242"/>
            <end position="276"/>
        </location>
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            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
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        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OEJ85216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="341" mass="38967" checksum="170717766B99125F" modified="2017-01-18" version="1">MSNPYQSNPYETAAPQEEYEMNTGQANYQNQYQNQYQNPYHEQTEEESNPYAGNAAPVQQTTTTTTTTQTVARPTGEQGDVFVGFMNNINEIHSNLETYQQIINNVDVLQKRLLTEINLEQEAQLRAQLDQLSSNAQDLQYQLKNEIKEAQRQALHDSSMQAQAENTRQKFLKLIQDYRIIEANYKDLNKEQAIKQYKVIQPEATDDEVEQAISDVGGQQVFSQALMNANRRGEAKTVLAEVQQRHQELLKLEKTMAELTQLFNDMEQLVIEQQENIEVIDQNVDVAQKDVEQGIGHTEKAVVSAKRARRNKQICYIILFVAFAVVVVVVVVPSVVVTRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REE5</accession>
    <name>A0A1E5REE5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Calmodulin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2178</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CALM/Myosin/TropC-like"/>
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        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
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        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
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    </dbReference>
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        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="10"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="46"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="83"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="149" mass="16304" checksum="283E6305E0251B3B" modified="2017-01-18" version="1">MSGANNLTEEQIAEFREAFTLFDKDKNGSISSSELATVMRSLGLSPSEAEVADLMNEIDINGNHTIEFGEFLALMSRQLKSKDSEQELLEAFKVFDKNGDGLISAAELKHVLTSIGEKLTDAEVDEMLNEVSDGNGEINIQQFASLLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REN2</accession>
    <name>A0A1E5REN2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AWRI3579_g1920</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REN2">
        <property type="interactions" value="350"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REN2"/>
    <dbReference type="InParanoid" id="A0A1E5REN2"/>
    <dbReference type="OrthoDB" id="3972647at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051186">
        <property type="entry name" value="RRM_HNRPC/RALY_subfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13968">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13968:SF26">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="291"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="517"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="660"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="49"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="82"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="106"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="169"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="530"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="660"/>
            <end position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OEJ85361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="717" mass="79155" checksum="E41CA3F18319ED3B" modified="2017-01-18" version="1">MSENILDNVNDLLKNLQQHQKQGQTDGEQHAIEESNTVASENSNEENDDSVKNXLEEAGQKDEEDNAEVKEENIHEXDEQKTTTSDNIDADSNQEXAEEIKKDEREXQEEEEEEEEEEEDEKESQQEDEKESQEEEEEEEEVEGQKEEEENGDDDDDENEESETKNEQEDGENGEVEGQEEDEDIDQELMQKQLDLIMGAGILNKPVFQDLSNEQKLYSLIKLINSEDATAMPNISINVSPPPPPGVVIGLNGRPRPDLNRAMSEEELEKYQEYLNEEKNITSVQYFPPRSRLFIGNLPLRNVTKEDLFRIFYPYGKIMQVNIKNAFGFIQFDNPKSVEDAIAIESQEINFNKQLILEVSSSTARPQFDHGDHGANSSSTFMSSAKRSYNEVVNNSEDFSQQQNKKNRRNRAVQCQIFVKRTADRGFANEAFNRFKYVTNLQIDMSFLKPRMDLKDMIQEAAEAGVWGVVLVNKTNNLDISTFYRDHNNEIKYNEYIGISIESGIALFNQLKQTGGATGPSTGIPPPPMSVGVPPVGAPMNNYMMSPPPQQQQQGAAMAPVASGSNNSPYGNYAPLSGQQGSFNAGPMPPQGGSSGASSYAGYPPNAPQMPFNQASANSPPNPAGFPGQAVGGQNPSQQQLLSQLQNLPPQMLSSLLASAQNQPPSGAXGAAPMPAQPMNMQPPNASGSNSPGANNGNTSATAKVQSLLDSLAKFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REP3</accession>
    <name>A0A1E5REP3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dilute domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2285</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REP3">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REP3"/>
    <dbReference type="InParanoid" id="A0A1E5REP3"/>
    <dbReference type="OrthoDB" id="3972274at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051020">
        <property type="term" value="F:GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052072">
        <property type="entry name" value="Vascular_dev_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16027:SF6">
        <property type="entry name" value="DILUTE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16027">
        <property type="entry name" value="DILUTE DOMAIN-CONTAINING PROTEIN YPR089W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01843">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01132">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51126">
        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dilute" evidence="2">
        <location>
            <begin position="193"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="627"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="633"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="646"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="659"/>
            <end position="668"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="708" mass="82201" checksum="91EDAD9BD6331E1F" modified="2017-01-18" version="1">MDLWNTAETTADNYTHGKENTEHTFQLNDDPVMKAIDLQTAGFSTNXDDLNGPNSSLFDVEKKLDTMNLDNDFYSADTEQELFQFDYSKLQPHQYIVFQDYDIVNILNYLVSLPDKYPHDITIPATIIYQCSRYIDLELKNSNYLANFIDLVCQKIQSAVQPPTVNTPSSSQXAHIDXKXEKKKQXTTQQSKPQRVDVVKKSYWLGMTNLLYFYFYKEQSSWFYKKNPLVLQKIVELLKFLVVQVCVSIYENITDDLIEEVLFNYTSIGEFQETLYKSKWNIFNKKGKKKESKDKSHTQNPSFSQEEANGISDRETSMDAPESRSPLKNSSYDEILKYLYPPSLEEQFKSPLKICQIYSALIYCLDLHSVHKLLQQQCLSLSIKWFATFVFNKILKNKSKTLCCRAKAMQIRLNLTMIQDWISANNIVLTKPTNSIDVYMWEKFPDTLILDSEVKLKNVLHVQHTNKPSQAVLTDEQNALFYYQPLTKVSNFYFDKVFELLEWLQVMTTLKDEQSLKATLLLINNLSVTQLLRTVDKYRYEIDEPEFKMKKVLKNLQQEEIRNSSPSARSEKQVYLPEREFPNGLVLPTMVELLPFYNASRDFIPLLPLEIRDCIETMHDTNEALHKKKSELINPNDQKYNAQEESAQEEATDDDNNDNRSTGSSNENAQKEYCPTNMFDNITVPTQAVAKDFVSDTAVIPGIEQNPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REQ2</accession>
    <name>A0A1E5REQ2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AWRI3579_g2452</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AWRI3579</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A1E5REQ2"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="54"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="2">
        <location>
            <position position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OEJ85389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="464" mass="53812" checksum="4DB00B1B1AFFBC42" modified="2017-01-18" version="1">MMSKELTATVTHNIAQISTLVGRDQNLEDLQKFIPCNFHNSGKYLCCDCASIQMLKSKINLETLRKEVAVLQETIDTILDNSLRKVKVSAVNVISKDXLPEYAQNNKQLFDGIRLLQSEMLIFQIRNKKQDIRLLSLKVKNMETRIISNLKLKDKMFKKNLNLPLNNSSLSFLSAHEQSLQTVHKNISETDDKIQLFKQVLERQKHTKFKELTEWVNITYSNKHNCYYFNNTVPIPQYIKTVFVINTNTALWQDGQDTQEDYVSSTFLLEYLERFLKTCLQTTIDTPECSDFERYSFQYKSPSSKKSDLENNKLRSNSVTAKSNVYAYDALISRLTFIVFACYKILQTKKQLLPAQGPTKKLSHKLARAPNTTNMGKTFSNWMLSEINYLGKLVHATCQPWNISSPETNDLRNHEGSRSYNVEYIRVPAHYFDMQDQEVINHLQTLLAGIVDEKNEKEKWMLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RES3</accession>
    <name>A0A1E5RES3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Proteasome activator BLM10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g2420</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BLM10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RES3">
        <property type="interactions" value="367"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RES3"/>
    <dbReference type="InParanoid" id="A0A1E5RES3"/>
    <dbReference type="OrthoDB" id="17907at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016504">
        <property type="term" value="F:peptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032430">
        <property type="entry name" value="Blm10_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032372">
        <property type="entry name" value="Blm10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035309">
        <property type="entry name" value="PSME4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021843">
        <property type="entry name" value="PSME4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32170">
        <property type="entry name" value="PROTEASOME ACTIVATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32170:SF3">
        <property type="entry name" value="PROTEASOME ACTIVATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16547">
        <property type="entry name" value="BLM10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16507">
        <property type="entry name" value="HEAT_PSME4_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11919">
        <property type="entry name" value="PSME4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Proteasome activator Blm10 N-terminal" evidence="8">
        <location>
            <begin position="98"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome activator Blm10 middle HEAT repeats region" evidence="7">
        <location>
            <begin position="543"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome activator complex subunit 4 C-terminal" evidence="6">
        <location>
            <begin position="2139"/>
            <end position="2226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1799"/>
            <end position="1818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1801"/>
            <end position="1810"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="2226" mass="256595" checksum="E24B36E937B6819F" modified="2017-01-18" version="1">MSAEQNQEKPPPEKLTNKFLAEVRTGRTFRSNSSNQGTKKPKISSLGQSASVTNLREYVKSPVRAKSATPLHALSQQQPNFPRKQQLLFNGMDSAPLMKQRLKHYNLDFVEDAAEHTKNVFDPKSQWFCRDVVPDYKPEDYFPHQTETHLDQARYLCHILVNLYIAISSKDIQGLLNISSKELAQFKSDIDNLTMNTDMFRLADVESSDMEDNASAFDDSQAEYDTVPDEDAAADEEGEELHDEEFDEPEFVHSGKITVKSSSIVNVNHWTNELRNCIHFDIPITLRKSLAVVYYYLALVRGQKVFRQLHTEVFELLVCTDYEGTNFTELLSEAGLILDYAPLLQLFEEYLPNPESDYQQYDIGNSGQLKLFKLMLKHAHNARPFFNKDDESILPYLMEFTISSFSPATLSIVSPIITSLLPYHFNVESNITDIFPFIFSVWKNAPITTVLDTHLYEVIGDVSQSAYFQFLSEENPRIVTRSHLSFGEYGLLNEGQFQFVINRIQNHLRNDFQIVSFSRTTKPLIYAINGSNSRQFFEQLGLLIQSIETFVHPSNSGRWTKICAKFIYAFIKAYHERFIFESKASSKYRTELKLNEETHSKLIETLIGTLLLGSQNKNTDIAHYYISGLAYLLNLKSQSNHLIYDKVLIDVYESLTDQYVNSIHRVFSSLKQFGRIVRFMVMDDLYRIHITNILSMIVTKLDSNDPVLSNVLVNTIASISCYVPVETFVKEDEYITFESTTLPLIEQHLYYIKENGSSSGFPVANADLELAFRASTSVFEAILTVYADKLFSFVDADLEEYLIFKINQTTMLMIESMSKELFDKFVRTFDRLFWDGNYLTVKKPNIDILGLTAGAIVKRDPHKYVRKFFDSIVLQINQELERGAGSVRSSKEVEQRDVKLVAMLNVLSEIFRHSHAQLLEIKSEILALLKKIYTSITNPPIDIITSLLLHNLLSSLTTTEIVDYNLFQKEAQVPINEKWGAYQFNEKKFEKQNMNFEWHIPTSAEVDFAIQIMRTFLELSQTQLSEIISNPVHDTKQTDTLRKYILILTHTLSGGCLLFDPEFNKELKQHKFTSNLGEKLLLWKNVKSNNIDDESVENFAVGKDNGEDDDLSIEEDDEMVMEKNFPVEVIENEDVMMEDFDSAMPSGMGTPGPGDHYGSATGNMSLMSSEFTFRELSVYTINYFFGATRAQKFSDKRYEEVHKLRSDVGLLFHKIATFLKEHYENNTNILQIFLHGLKVWFTDVGQETIFNEDPTACLDYDFLESLQSLAHVTDTYTRTFIASRIQYFYQTRVYLRSTNRVPSKMEKTLLNDIVDLSTSVYPNVAKSAQACLFGTMRQLTGSYRYVVENMMNFFDEALTMQNELKLESILSAMSSKKFLSKIYNDYKNISRVINLLLKASLTKHHEVSQTASRLLALITTSLKIPSSVCLIDMQKMETINPGDEYIALQVEIVQKAKEAKRNEYMDLLVDLRADMLNIFEKDQKTLPWKHSLIFIEILTKLQSNMEFSIDEKTVVKLFEQTEQLHPSVVFNATNSIMDIVNRIFLKSDLCYNISNKFDPNFHRKFVEILDTKQPNFASFYQKEMENFQKPKFFIDNKTYVGFFSWGRELKVVKNVTQLDISTMRESDKLTLQKIGSMINKDYLKKYTDFLVSENEGKGSFSHQNVDFIVMIIHFIEIGFTTDISYTDVLELSSSIFDKYDKAKIIMSAEIFCGIILASKLTAQMDLVKRDDFLNKYMDDLFDKNMNKDTLEVFQVVFWWLPNHVDMRRCYPVFVKLTSLEDVLLSVEQNSVEHGIEKLTDEQSSLQEQESVSYPAAASGKXEELSPSLINARVSLFRHMVMSMNHRTPFIDVFANKLVFDYPYELVRRGISNCINLMMICDCMRVFDNVSDMIAYIAADTSDGLGNSLKVIPEWFDTLFVNEMEVINKIYCENEEFFKTAKPQDXVKHEFYYRSNTMYHLIFGIITNSIVPIGKYLSQYVVPFLDRLYLMKDVCKLGNIYPDLVYMHIAKTPMDRLVIEDVLANLLHNDQIFNTPHGIRVQMTFFEKFYSQKMFLLRQDEKLEILNYFVDNLYNAKSXEVRMKSSEVLSGIIHTISLHENGNLDQVITKLITKFETKLGADLKLKEKQKIIKGNPKNVIEIHGSIIGLGALIAAFPYISPLPKWIPQVLSNLSSWAKVSGIVGSSSKDIISNFKKIRSDTWHIDRESFTTDELENLEGVNWRSYYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RET5</accession>
    <name>A0A1E5RET5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Uridine kinase</fullName>
            <ecNumber evidence="6">2.7.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AWRI3579_g1732</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>cytidine + ATP = CMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>uridine + ATP = UMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16825"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the uridine kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.48" evidence="6"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RET5"/>
    <dbReference type="STRING" id="56408.A0A1E5RET5"/>
    <dbReference type="InParanoid" id="A0A1E5RET5"/>
    <dbReference type="OrthoDB" id="738517at2759"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00637"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00579">
        <property type="reaction ID" value="UER00640"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043771">
        <property type="term" value="F:cytidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004849">
        <property type="term" value="F:uridine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044211">
        <property type="term" value="P:CTP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02023">
        <property type="entry name" value="UMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000339">
        <property type="entry name" value="Uridine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000764">
        <property type="entry name" value="Uridine_kinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004018">
        <property type="entry name" value="PRK05480.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00235">
        <property type="entry name" value="udk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00988">
        <property type="entry name" value="URIDINKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="7">
        <location>
            <begin position="13"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OEJ85428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="253" mass="28407" checksum="DAEB309C54E22127" modified="2017-01-18" version="1">MSAITTQHQQPYIIGVGGPSGSGKTSVASCIVKEINSPNTVLVTMDNFYNPLSEEQHNQALENNFDFDNPDAIDMESIYQCLKNLKKGLKVDIPVYSFIEHNRVPNEKITIQDAHIIVLEGIYALYDPKILEMMDLKIYVDADLDICLARRLTRDILSRGRDLDGCLKQWTKFVKPDADKFVRPTVKAADVIIPNFIDSNSAGIRVVISHLKNKLEEKEKMLENGQSLDSVNKEITNEIANELTSEISNTVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5REU9</accession>
    <name>A0A1E5REU9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Abhydrolase domain-containing protein IMO32</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g2129</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5REU9">
        <property type="interactions" value="470"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5REU9"/>
    <dbReference type="InParanoid" id="A0A1E5REU9"/>
    <dbReference type="OrthoDB" id="8119704at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46118">
        <property type="entry name" value="PROTEIN ABHD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46118:SF4">
        <property type="entry name" value="PROTEIN ABHD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="96"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="355" mass="40304" checksum="4B065BFAAB98550D" modified="2017-01-18" version="1">MYNCRNLLFTVSKTKGGSKQVFHNTKLNLNQLGKFRYILRQNRNYSSVPKQAEGQGLYGSTEILNQELCADSIPSVPLVSEVLKYEKRDSDATESPVIVLHGLFGSKQSNRTMSRFLVSGLKRNVYSLDLRNHGESPHIARHDYPAMANDVEKWVVEHNFXHKPILVGHSMGAKVAMSCVLRKPDMFAAFCAIDNAPATTMPTPKFPKYVKQLLAIVNNPECSTNKQALEALGKVEQNLMIRQFLMTTLTKYKDEKTGKSRYREKIPLELLNDAIVKGNIANWEFNSWVHKWSGPALFIRGTESTFCSDDFITDVARFFTNFEIRDIKGSHYVNTEKPQECAEAITTFIEXVEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF01</accession>
    <name>A0A1E5RF01_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g2373</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RF01">
        <property type="interactions" value="259"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF01"/>
    <dbReference type="InParanoid" id="A0A1E5RF01"/>
    <dbReference type="OrthoDB" id="162894at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000340">
        <property type="entry name" value="Serine threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000408">
        <property type="entry name" value="Serine/threonine-protein kinase RIM15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE GREATWALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="13">
        <location>
            <begin position="138"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="874"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="14">
        <location>
            <begin position="1295"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="12">
        <location>
            <begin position="1625"/>
            <end position="1739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="21"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1041"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1136"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1407"/>
            <end position="1433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1467"/>
            <end position="1487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1503"/>
            <end position="1575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="42"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1043"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1407"/>
            <end position="1421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1471"/>
            <end position="1486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1555"/>
            <end position="1575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ85143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1763" mass="196886" checksum="7D25E6EFE4A5EDD7" modified="2017-01-18" version="1">MKKSKIKLPKFKKTLLELNKFGRKTGSTNEHGNKLATTFGIKTKDKSSKNDDKSTKHTSIDSVLDDREHKEGHHSDSEVMNQTKLNEALSITNSNKEKFEVQLPIPSANVPISASSNKXNLTKQQSQSLSGSQTSSSSTERYVELLQKASNSDTTILIELDLEGNIKYISEVWEKIIGTNSVEMVGRPISEFVIGSDFDKSVFERTTKLMLEDDSSYRIKFTLEQFTVDKNLVSEKVENILSAQCVQFEEEENPVEIDVTTTSSFKNXNFIELEAQGIVITDLAKRTPLHSMWIIKPFYAVDEYDNLPFSLAEKLGFGAIIFQNYLNEIEKLKILHEKDLPPYRREICRICETAIITWWLESHCSMCLLEHNLYNNISSLHDNLVDNKQILLKIKRGLLALKDFANSSENKLNQYQLDIEPIQYKDIYIDVLSLDFSLIPTLNLFISCCDDAININNSELKSFQSFRFDNIEFDFSPTSKQNIEHVKNWKARLAHLYNGIDSIRLQDLKMVFYDIQTSSELKIMQLERLQNTQKYHLKETSEVTLYTQELVKQKIQSNRLNIPPNIQLSDSSFTPVGDINFDEHKLQYSDYPVSSGQXMEENMAIPAPQKLQKPTLFNQAYLQGSDESIPSAVVLHSAHSSRQAERVYSPKHSDEAATKQLDLQNTFNDNANSNDLAKNISFGSLQEEQHTEPIVTIQEPEDVSHVGVPPGSRPXSLLISNHLQSIPTSSSEDSSTDLGAFAETNKNATESWSRGSSQNTKSIKRLSAATRYNALASISATPRKGSPLSTVGNINTPISVIKKNPTAKTIMEKSPLASPFMPPLESSDISIHHTHASTSDSITTSMQPMSPLLLANESSGLHHRHSSNPQIFDYDIIKPISKGAFGSVFLAKRKLTGEYFAVKVLKKSDMILKNQVTNIKEERAIMMSQINKTYTAKLYATFQNKENLFLVMEYLPGGDLAALIKNVGILPEKWTKQYISEVIYGVNDMHQNGIIHHDLKPDNLLLDIRGHVKFIDFGLSRRGLVNRQRHDSFGSQGMIASGSPGSQHTANSLHNSTGISLGDSLRKKTTTDNASYFSLPSASNAAPRVKIVESPNQGLTSDMQKAFDNNEVLEAAKMQESGSHYTFSVDYGSRSATPSVGGRKNSGKFNGSDATTSTAISLSKASRGSADGDFVIYDPKDSNKQQRFLGTPDYLAPETISGAGESNLCDWWSIGCIFFEFIFAYPPFHASTPTEIFQNILQCKIQWPDFENEEEERFFISAEARDLVEKLLVLEPSKRLGANGVDEIKSHPYFDGFDWSKVYEEKAEFVPNVAHPEDTDYFDPRGATMNIDLHLSDSEGYASEEATNALNLDNIDTLNDFNGDGYNMSELPSSIHHSSTVINSECAGSKLEPTSVASILSPAQFGSSDNGSISSSTNTSITRRRANSTKLNENQTEFGSFSFKNIGALSKANKDVINRLKNEHLNEKGQHLSSASASSVNSHLSNQNQHLKNSTFSGLLKSSFSHSPVRRDSAESGRASNNSFLSETHGDDYHSSSGAPSEISVSKLARETDSGNRSSMSRAYSTGRNHSNSSISLDALSNDEERFDALAQLKILRKQERKKANRKFSNEDAQSSVTQLEFNLDVLVCESIPIHSFFITRNLEQLGCRVIAVDSGDELIRRATSNVKFDIIFSTYIMPNLNCIDLFKLIRNTNGVNCNTPFVVITAYYNEAAKLKLFDEVVEKPATKGTMRKLLKKYTMKKVQQCEETLISDVESEIFYETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RF24</accession>
    <name>A0A1E5RF24_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">54S ribosomal protein IMG1, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AWRI3579_g1722</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RF24"/>
    <dbReference type="FunCoup" id="A0A1E5RF24">
        <property type="interactions" value="280"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RF24"/>
    <dbReference type="InParanoid" id="A0A1E5RF24"/>
    <dbReference type="OrthoDB" id="432645at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001857">
        <property type="entry name" value="Ribosomal_bL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038657">
        <property type="entry name" value="Ribosomal_bL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680:SF9">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL19M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680">
        <property type="entry name" value="RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01245">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OEJ85501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="178" mass="21322" checksum="9D03C746B712BD29" modified="2017-01-18" version="1">MLKNFINPLKVFTRNYQLKTTTHKTIDVYPPREPVTMKTQRYRQFVQKDVERLDPSHWRRDLLSAKTPQTVVRTGDIVRVVYHKRPNMKMDDLYGYVLGINKKQHGTLLGSSLLLRNHFAKTAVEVRVPVFSPLIERIDIIKRPERRRRRNKHYYIRDQKTDVGNIEGDVKRRRQLQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFB2</accession>
    <name>A0A1E5RFB2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Putative PH domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g2279</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFB2">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFB2"/>
    <dbReference type="InParanoid" id="A0A1E5RFB2"/>
    <dbReference type="OrthoDB" id="26740at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21675">
        <property type="entry name" value="SMP_TEX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466:SF19">
        <property type="entry name" value="NUCLEUS-VACUOLE JUNCTION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466">
        <property type="entry name" value="TEX2 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="SMP-LTD" evidence="11">
        <location>
            <begin position="295"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="41"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="513"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="583"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="680"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="780"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="513"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="606"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="622"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="652"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="680"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="712"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="747"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="794"/>
            <end position="804"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ85567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="866" mass="97636" checksum="87641A0B75A9BBA4" modified="2017-01-18" version="1">MSSWKSYLFTFLLGFISFPAATIALVVYYFKLQKDFPNFDDGSENETKQPLLQGQNAQNSDKEELKESGNPAFAEAKNDNLLWFKVDPNLKAGVLSEKTGVQVYKKGEIVITKQCFYHPMTIPEDKRDTPEYPTTAKLQRNKNKYYCVLKHGNLFLYEDENHEGLTNAIVLKDAIVTIWPNNKTIKENSLFTKRTCIAIFKNRLYNRETKQIELPASSAPSNSMFFCYFDDNSVKEDWYFALLDASKTDVSSSSSSSSSSSSFNNICPEMKPPVTAHFKTKDMLKLIQIINSTEGQLSTKWLNVILGRLFLSFKENEKMSNIIKNKIYTKLSKINTPGYFDGFXIIHLYPGDVAPVFTNPKTRQISIEGDCEVSVDVAYAGNLVLQISAILSVPMGFKKPKSLPVDVKLILKKLSGTLVFKMKKPPSNRIWYCFEQDPEMDLEIEPLVSNRNFNYNMITSLLKNKIQDALKESLVFPFMDDIVYYTLEEYQDYFYKGGVWDFSANAVFGEMNEKQXSSKEDNKPTKQSLEAERTLVDGLQNKREKEEEKEEEKQEENNSQEHNNNSNEKKITSKLKEPFSAXLSSPHEDLENNSLSKTPGSPVHKTQSNNSEQSSNTSFTSKELKMDDLKEKTMEKMGQFKSLFKNKDQKADLTNNEDSTNKYSKKMGKWYNNLKESASNYGNDLERSPQNPFLNKESMSSPLQHKSEGSNEENVSSVGSTASNVVSSASGAGNITQEPSSEXAVMQSDTNSNAQNVKKTDTQNDVAPYKAPEMITSRRTKRNSVTVTEVPKTPESNPSLDNNIGSSVPAGMFVKKDILMTSATLSPTGNNISGNVARTKTVKLNGHEKEFMGSHRPVPPDLPPRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFB7</accession>
    <name>A0A1E5RFB7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Translation factor GUF1, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g1983</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.n1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion inner membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the GTP-binding elongation factor family. LepA subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFB7">
        <property type="interactions" value="735"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFB7"/>
    <dbReference type="InParanoid" id="A0A1E5RFB7"/>
    <dbReference type="OrthoDB" id="1074at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097177">
        <property type="term" value="F:mitochondrial ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01890">
        <property type="entry name" value="LepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03709">
        <property type="entry name" value="lepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000078">
        <property type="entry name" value="Elongation factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000015">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000007">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2570:FF:000001">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000004">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2570">
        <property type="entry name" value="Elongation factor 4, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00071">
        <property type="entry name" value="LepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006297">
        <property type="entry name" value="EF-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038363">
        <property type="entry name" value="LepA_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013842">
        <property type="entry name" value="LepA_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035654">
        <property type="entry name" value="LepA_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01393">
        <property type="entry name" value="lepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43512:SF7">
        <property type="entry name" value="TRANSLATION FACTOR GUF1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43512">
        <property type="entry name" value="TRANSLATION FACTOR GUF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06421">
        <property type="entry name" value="LepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="10">
        <location>
            <begin position="65"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="74"/>
            <end position="81"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="145"/>
            <end position="149"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="199"/>
            <end position="202"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="672" mass="75523" checksum="3486829101F738E1" modified="2017-01-18" version="1">MLRSVTSCSVLVKAFPAQISSLWHYKAFSSTANLSNENSAHDTSLQEKILGSKELQDRIKAIPLENYRNFSIVAHIDHGKSTLSDRILEYTNTIPAATDAEAQKKRAQILDKLEVERERGITIKAQTCTMIYPYKGQDYLLHLIDTPGHVDFRMEVCRSYASCTGALLLVDATQGVQAQTVANFYLAYSMGLKLIPVINKIDLKQTANIPRSKQQVQEMFELDPEGCVMVSAKTGLNVKEDLLPAIVERIPPPDLAKVNPKAPFRCLLVDSWYDTYLGVVLLVYVVDGFLKKNSKVVSAHTNIKYDVKQLGVMYPDMTPQDSLKCGQVGYIVPGMKNSKDAHIGDTFYQVGKETEPLPGFEEMKPMVLIGAFPAEGVDFKKLEDDLYRLTLNDRSVNIQRETSNALGQGWRLGFLGSLHASVFKERLEKEYGSSIIITQPTVPYKLVXKDGTEKMITNPDDFPDLAAKKSRVEKLMEPYVECLMTAPQEYLGDLIELCENNRGKQLDLQYIDSTGQVLLKYEIPLAHLIDDFFGKLKSCSKGYCSLDYEDKGYQQSDLVKLELLVNGNSVDALSAVVHRSQVQSMGKKWVEKFKEFLKIQQFEVIIQSAADNKIIARETIKARRKDVLAKLHASDITRRKKLLVKQKEGKLKLKSIGKVAIPQDAYAAFLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFC8</accession>
    <name>A0A1E5RFC8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">AP-3 complex subunit delta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AWRI3579_g1914</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Adaptor protein complex 3 (AP-3) is a heterotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFC8">
        <property type="interactions" value="633"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFC8"/>
    <dbReference type="InParanoid" id="A0A1E5RFC8"/>
    <dbReference type="OrthoDB" id="10264595at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030123">
        <property type="term" value="C:AP-3 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030665">
        <property type="term" value="C:clathrin-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000251">
        <property type="entry name" value="AP-3 complex subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017105">
        <property type="entry name" value="AP3_complex_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22781:SF12">
        <property type="entry name" value="AP-3 COMPLEX SUBUNIT DELTA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22781">
        <property type="entry name" value="DELTA ADAPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037092">
        <property type="entry name" value="AP3_complex_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="11">
        <location>
            <begin position="44"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="679"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="738"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="800"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="847"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="903"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="679"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="700"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="747"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="867"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="910"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="921"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="12">
        <location>
            <position position="832"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR037092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OEJ85608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="947" mass="108140" checksum="D7024A60C50D3807" modified="2017-01-18" version="1">MSFLDSRLRPFGLSFEKTLKDLITGIRSNNSNPEDLQNFLTKAVQECKEEVKSTDMTKKSNAILKLSYLEMYGFDISWSSFYVLEVMSSNNFQQKRVGYLAAQQVFHSDPNILMLATNCLKKDLNSMHTVEVGIALNGISSIVTPSLARDISKDLVRMLRHGKSYIRKKAVASLFKLFLQYPEALRDDFQIFIEKLHDEDSSVVSVVVTVICELSKLNPEPFMPLLPLFYDMLLDIKNNWITIRFLKLFTTFSKXEPKLRKKLLPKILELISKTDAKSLIYESINCIVRGRMLEHTDYDVAAQILKPLASFCDSRDPNLRYVSCVLFYEIGKTNHGFISNFDQLIFKLLVDVDISIRSKAVELLESVIDDGNIVEATGILLKQVVMDEEKLNSRMSGKEERVEIPNFYKIKVMQILLHLFQKNDYENISMFEWYIAVLQDIANIATSVDLGRDCDLGAKIGEHFRNIAVKVPDMRGQVTMALVKIISTDSMFTSIPHILSDTFWCLGEYSSVISNCDDLIDLLINNKRALLVNLPGDVLKNIFPCLMKLFNNWINSDLHETAEVATISDVCRKLNEFFELFIQSSEIEIQERSVEQLEFFKILQEALNLSNGDEEQEQELPMLATSILPSFFDSWEIQPVQQGQQKKLQNRIDLSWLEEESFLSDEMKKDLDALIDDELVSDEDDVEEDDFDLSKPQDYDQYDSSDEEGFTENKILGIPNEEYRRLKEEQKRERMDNPFYLDEEDSNSNLQPADLNFDSSNSAKNNETSTIDIKVQGIFQNAGAKKTKKXKKAKMKIISDSKLDLDDMDESTSKKSGNKVAFPTXGQXRAGDGKIDLSFKNKLKGFDFSKSSVSTPLXDDDENGSTTHKEDIDLNQLREDFDAGTKLVDDGDDAEEEIIVVKKKKKKSAKKGEDGDATKTKKEKKKKKKKTKNSSKXQLVEINDESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFI4</accession>
    <name>A0A1E5RFI4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Vacuolar-sorting protein SNF8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AWRI3579_g2324</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AWRI3579</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNF8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFI4"/>
    <dbReference type="FunCoup" id="A0A1E5RFI4">
        <property type="interactions" value="656"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFI4"/>
    <dbReference type="InParanoid" id="A0A1E5RFI4"/>
    <dbReference type="OrthoDB" id="283883at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000814">
        <property type="term" value="C:ESCRT II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016689">
        <property type="entry name" value="ESCRT-2_cplx_Snf8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040608">
        <property type="entry name" value="Snf8/Vps36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12806">
        <property type="entry name" value="EAP30 SUBUNIT OF ELL COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12806:SF0">
        <property type="entry name" value="VACUOLAR-SORTING PROTEIN SNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04157">
        <property type="entry name" value="EAP30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="3">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OEJ85668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="275" mass="31684" checksum="06478DD063248BE5" modified="2017-01-18" version="1">MNELNSELTGLRISTPPGEQGSSTTNKAPYNYDPSLFDKQKQQLNYQLQVFKDQIIQFSKIHNQELKENLDLARQFVQLCSQIGINFSQLVDNKSTYLFHPMDYYYNLCIELIEVVRNLETMNGGLTSYKELLFRLNESNGSSITDIKQQKTNNNKRSAQDIDKALEMLESLNGGFTKMVIAGKSFIQSVPLELTKDQSLVYEVCFVLGYVTVPILRDNYQWDKIRCKQVLNKMVSDGLLWIDKTEDEANEATLNTSNKFRGEVKYWDPGWIVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFQ1</accession>
    <name>A0A1E5RFQ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Vacuolar fusion protein MON1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AWRI3579_g1763</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Required for multiple vacuole delivery pathways including the cytoplasm to vacuole transport (Cvt), autophagy, pexophagy and endocytosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endosome</location>
            <location evidence="4">Multivesicular body membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 4">Prevacuolar compartment membrane</location>
            <topology evidence="1 4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Vacuole membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the MON1/SAND family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RFQ1">
        <property type="interactions" value="535"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFQ1"/>
    <dbReference type="InParanoid" id="A0A1E5RFQ1"/>
    <dbReference type="OrthoDB" id="272411at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035658">
        <property type="term" value="C:Mon1-Ccz1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032585">
        <property type="term" value="C:multivesicular body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043972">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043971">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043970">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004353">
        <property type="entry name" value="Mon1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13027">
        <property type="entry name" value="SAND PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13027:SF7">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN MON1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19036">
        <property type="entry name" value="Fuz_longin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19037">
        <property type="entry name" value="Fuz_longin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19038">
        <property type="entry name" value="Fuz_longin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01546">
        <property type="entry name" value="YEAST73DUF"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="FUZ/MON1/HPS1 first Longin" evidence="6">
        <location>
            <begin position="250"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="FUZ/MON1/HPS1 second Longin" evidence="7">
        <location>
            <begin position="466"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="FUZ/MON1/HPS1 third Longin" evidence="8">
        <location>
            <begin position="679"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="736"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="8"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="744"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="757"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="9">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <feature type="unsure residue" description="D or N" evidence="9">
        <location>
            <position position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF19036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF19037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF19038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OEJ85697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="839" mass="95810" checksum="EBFBF6D09FF4F0E1" modified="2017-01-18" version="1">MSTRKNDNFPSSPSITPKRPTNTRYQIPHNAQLSSRPVTPLKPVLDTTNVININESLPQQRDNQVLSRTSSLYDLRSATRSAVTSPTNASQRYRSLNEYTNHAGGSRNAYYDTNDNASIRSNVTALTTNLHQELMQSINTDNLSLYDYYGEEPQLHPLPITQTHAGSMRLQFDEDIQEDDISSFTGEDXADEQDTMQRLPVPSITPMFSSSRLDQENAHLNNADFLPELTLPDLDSHRANFVSHTPYTDKNFFILTSAGKPIFSMKHDIILNEENDDVDMNDDSQNNQDELYGLSGIINTIVEFFKIKKQCNLNSFEIKKAAGNGKVKTCKFVFLDKSPVVLMAYSELEYESELHLSDQLDFLYSFLLSILSKNKLDRLFDKRENFDLRNFLTDLDFQKMVDXTKQICFQFHPQFLFYQSSSLQIYPMKKGFRTALHRLMLNTYMKSLGFKDELITNNGPINASSTLLYGMIVSFPSTQLVSILRPKSHSLHTTDLHLLFQLIKTQYFLNQEGANKENGDKKDMFVTDENTATKMGDTYFYQPICFPKFNSNGFLYCYIKFFHNDAFAHNSLESSLESTFATVLICSKKQALPYCQHLDNLIQQAIIQETTTGKYKKLFTTTDKDNEVVSTNGEAGVTRNGLMSMDSRWSTYASHPYNNVQQVNGMSKNAIQYPELPXVYHFIYKSKPLVQHITPTISENLMHYYLFLQSQALDSDQQPLDKKSTLIAAKITVSGAGNEESGGTIATPTTMSENDSHNNNSHNNNSHNNNSHNNNSHNNNSYNNQTIMGICWITKNFEFYLLGNPNYNNKKELLMSSKKIIQWCYKNEQRMFIHDGAVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFQ5</accession>
    <name>A0A1E5RFQ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">60S ribosomal subunit assembly/export protein LOC1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">60S ribosomal subunit assembly/export protein loc1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AWRI3579_g2328</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LOC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFQ5"/>
    <dbReference type="FunCoup" id="A0A1E5RFQ5">
        <property type="interactions" value="394"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFQ5"/>
    <dbReference type="InParanoid" id="A0A1E5RFQ5"/>
    <dbReference type="OrthoDB" id="3973417at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037650">
        <property type="entry name" value="Loc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28028">
        <property type="entry name" value="60S RIBOSOMAL SUBUNIT ASSEMBLY/EXPORT PROTEIN LOC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28028:SF1">
        <property type="entry name" value="60S RIBOSOMAL SUBUNIT ASSEMBLY_EXPORT PROTEIN LOC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="147"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="21"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="42"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="204"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OEJ85676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="214" mass="24955" checksum="34FD98538945BE5B" modified="2017-01-18" version="1">MGTAKRVKRQTLMREVRPEMTQDQTAANQLSFNTGRKTAKKNPKDAAPNKKEQRRALLYGSQRKDKHKRELLRKQIEKDLPSLNRAVVPGLKLKRGKKGKKFVADHDSIILERLVKTVGDKQDQVDESKLEKSRRLEEIRDLKRKELERKEQEKQNKLDDKKNELKKTSSVARTMRRKNKRVAEKEAQREEEDLQKSKSEKTAAKKAKKSVSFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RFR7</accession>
    <name>A0A1E5RFR7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 5B</fullName>
            <ecNumber evidence="3">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Translation initiation factor IF-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AWRI3579_g2209</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="3"/>
    <dbReference type="EMBL" id="LPNM01000007">
        <property type="protein sequence ID" value="OEJ85717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RFR7"/>
    <dbReference type="FunCoup" id="A0A1E5RFR7">
        <property type="interactions" value="503"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RFR7"/>
    <dbReference type="InParanoid" id="A0A1E5RFR7"/>
    <dbReference type="OrthoDB" id="4928at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03703">
        <property type="entry name" value="aeIF5B_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000013">
        <property type="entry name" value="eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10050:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000112">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="entry name" value="Translation initiation factor IF- 2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003078">
        <property type="entry name" value="PRK04004.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF4">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="Initiation factor IF2/eIF5b, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 15" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="14">
        <location>
            <begin position="422"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="279"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="37"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="151"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="167"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="195"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="301"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="370"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OEJ85717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="1022" mass="113140" checksum="81AA1D31C1CC40E5" modified="2017-01-18" version="1">MAKKGKKNAPNYWDEEVEEDQQLQDQPQELGSEPTPAVSEASGAEASGAEASGAESVSASASVEPETAGADDIESDFMSTLKKSKNKKKNGNKKNGNNDNNDEEGQANGGKPVLKSKKEKEKEKKEKEKQKKKEQAAKKKTAGGAKNNDQSGDKKNGGEANKDSDANESASASASSSAPSKPKKKVPAGLAALRKQLELQKQYDEQERLAREEEERLEKEEEEKAANELAQKEEERKLKKEKEKAKKERLKAEGKLLTKKQKEEKKLLERRRAALLSAGNIKVAALEKANNGENADQESKKPKKVVYGKKKKKTKEELEAEQSAKKAAEEAAKQETENGQQEDADELVDDWEALLSGDEEAAVSDVTVEETPKEVSKPKTEAPKTEISKEVSPKPAAVDPKYDLYSAAANASRTKPKGNKDLRSPICCILGHVDTGKTKLLDKIRQTNVQGGEAGGITQQIGATYFPIEAIQQKTAVMAEYEEQTFDVPGLLVIDTPGHESFTNLRSRGSSLCNIAILVIDIMHGLEQQTLESIRLLRDRKAPFVVALNKIDRLYDWQTIPNNSFRDSFEKQTRSVKQEFEKRFNEIKVALAEQGLNSELYFQNKNISKFVSIVPTSAVTGEGVPDLLWLLLELTQKRMSKQLKYVSNEIEATVLEVKVVEGFGTTIDVILSNGKLREGDRIVLCGLNGPIVTNIRALLTPQPLRELRLKSEYVHHKEVSAALGVKIAANDLEKAVSGSRLLVVQDEEDEEDMMEEVMDDLTGLLDSVDNTGKGVVVQASTLGSLEALLDFLKDMKIPVMSIGLGPVFKRDVMKCGTMLEKAPEYAVMLCFDVKVDKEAEHYAEEQGIKIFNAEVIYHLFDAFTAYQKDLNDKKREEYGSQVVFPCVLNTIQIINKRGPMIIGVDVMEGTLKIGTPICAVKVDPTTKQKQILPLGKVASLEINHESVPEVKRGQTNAGVAMRLDDPSSQQPIWGRHVDEKDTLYSMISRRSIDTLKDPLFRDQVPKTDWLLIKKLKPTFGID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGM6</accession>
    <name>A0A1E5RGM6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Prohibitin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AWRI3579_g1092</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AWRI3579</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Prohibitin probably acts as a holdase/unfoldase for the stabilization of newly synthesized mitochondrial proteins. Involved in mitophagy; may act as an adapter for ATG8 that supports mitophagosome assembly. Negatively regulates the proteolytic processing of ATG32 via the i-AAA protease. Acts as a negative regulator of the m-AAA protease.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">The mitochondrial prohibitin complex consists of two subunits (PHB1 and PHB2). The subunits assemble into a membrane-associated ring-shaped supercomplex of approximately 1 mDa. The mitochondrial prohibitin complex interacts with the m-AAA protease, a heterohexamer composed of YTA12/RCA1 and YTA10/AFG3. The mitochondrial prohibitin complex interacts with ATG8 and the interaction may support mitophagosome assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the prohibitin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGM6"/>
    <dbReference type="FunCoup" id="A0A1E5RGM6">
        <property type="interactions" value="905"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGM6"/>
    <dbReference type="InParanoid" id="A0A1E5RGM6"/>
    <dbReference type="OrthoDB" id="275637at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045861">
        <property type="term" value="P:negative regulation of proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03401">
        <property type="entry name" value="SPFH_prohibitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000001">
        <property type="entry name" value="Prohibitin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000163">
        <property type="entry name" value="Prohibitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222">
        <property type="entry name" value="PROHIBITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222:SF1">
        <property type="entry name" value="PROHIBITIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00679">
        <property type="entry name" value="PROHIBITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band 7" evidence="9">
        <location>
            <begin position="54"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00057247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00063239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OEJ86059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="302" mass="33420" checksum="3A619C33F5D99AE3" modified="2017-01-18" version="1">MSNDFQRFAKQFQNSMNQGRGGFRAPMRAPGGSGLGAFAGLLFLAGGALLVNNSLFNVEGGHRAIMYSRLDGVKNRIFAEGTHFAIPWVEKPIIYDIRAKPRNVASLTGTKDLQMVNVTCRVLSKPDVNNLSVVYRTLGTDYDERVLPSIVNEVLKAVVAQFNASQLITQREHVSRLIRESLVRRAAKFNILLDDVSITYMTFSPEFTNAVEAKQIAQQDAQRSAFIVDKAIQEKQGLVVRAQGEAKSAELIGEAIKKSKDYVELKRLDTAKDIAEILAKSPNRVVLNNEALLLNTVIDARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGR1</accession>
    <name>A0A1E5RGR1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Farnesyl pyrophosphate synthase</fullName>
            <ecNumber evidence="6">2.5.1.1</ecNumber>
            <ecNumber evidence="5">2.5.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">(2E,6E)-farnesyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Dimethylallyltranstransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Farnesyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Geranyltranstransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AWRI3579_g1494</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AWRI3579</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Isoprenoid biosynthesis; farnesyl diphosphate biosynthesis; farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Isoprenoid biosynthesis; geranyl diphosphate biosynthesis; geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.1" evidence="6"/>
    <dbReference type="EC" id="2.5.1.10" evidence="5"/>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1E5RGR1"/>
    <dbReference type="FunCoup" id="A0A1E5RGR1">
        <property type="interactions" value="830"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGR1"/>
    <dbReference type="InParanoid" id="A0A1E5RGR1"/>
    <dbReference type="OrthoDB" id="10257492at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004337">
        <property type="term" value="F:(2E,6E)-farnesyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004161">
        <property type="term" value="F:dimethylallyltranstransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045337">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000006">
        <property type="entry name" value="Farnesyl pyrophosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039702">
        <property type="entry name" value="FPS1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11525:SF0">
        <property type="entry name" value="FARNESYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11525">
        <property type="entry name" value="FARNESYL-PYROPHOSPHATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01017">
        <property type="entry name" value="Polyprenyl_Transferase_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OEJ86099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000095728"/>
        </source>
    </evidence>
    <sequence length="352" mass="40687" checksum="C7BD81050150169C" modified="2017-01-18" version="1">MSAKEHNRNKFLSEFPALVNELTEILVQYGLPKDAIEWYQKSLEYNTPGGKLNRGLSVVDTYVILKGYKSVDDLPKETYFKVALLGWCIELLQAYFLVADDMMDKSITRRGQPCWYKVPGVSEIAINDAFMLEGAIYMLLKKHFKNDPYYVDLLELFHEVTFQTELGQLLDLITAPEDHVDLNKFSLEKHSFIVIFKTAYYSFYLPVALAMYVAGINDEKDLKQAHDVLIPLGEYFQIQDDYLDCFGKPEDIGKIGTDIQDNKCSWVVNTALKICNKEQRQILDENYGVKNSETEAKCKKLFNDLGIDKLYHEYEEKITTELKTKISQIDESRGFKGEVLTTFLNKIYKRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-01-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1E5RGV9</accession>
    <name>A0A1E5RGV9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">RNA polymerase I-specific transcription initiation factor RRN7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AWRI3579_g1135</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Hanseniaspora osmophila</name>
        <dbReference type="NCBI Taxonomy" id="56408"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycodales</taxon>
            <taxon>Saccharomycodaceae</taxon>
            <taxon>Hanseniaspora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Genome Announc." volume="4" first="E01287" last="E01287">
            <title>Genome sequences of three species of Hanseniaspora isolated from spontaneous wine fermentations.</title>
            <authorList>
                <person name="Sternes P.R."/>
                <person name="Lee D."/>
                <person name="Kutyna D.R."/>
                <person name="Borneman A.R."/>
            </authorList>
            <dbReference type="PubMed" id="27856586"/>
            <dbReference type="DOI" id="10.1128/genomea.01287-16"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AWRI3579</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRN7/TAF1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LPNM01000006">
        <property type="protein sequence ID" value="OEJ86142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A1E5RGV9">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="STRING" id="56408.A0A1E5RGV9"/>
    <dbReference type="InParanoid" id="A0A1E5RGV9"/>
    <dbReference type="OrthoDB" id="3971841at2759"/>
    <dbReference type="Proteomes" id="UP000095728">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070860">
        <property type="term" value="C:RNA polymerase I core factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001164">
        <property type="term" value="F:RNA polymerase I core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048540">
        <property type="entry name" value="Rrn7_cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033599">
        <property type="entry name" value="TAF1B/Rrn7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021752">
        <property type="entry name" value="TF_Rrn7_Zf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31576">
        <property type="entry name" value="TATA BOX-BINDING PROTEIN-ASSOCIATED FACTOR RNA POLYMERASE I SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31576:SF2">
        <property type="entry name" value="TATA BOX-BINDING PROTEIN-ASSOCIATED FACTOR RNA POLYMERASE I SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20644">
        <property type="entry name" value="Rrn7_cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11781">
        <property type="entry name" value="Zn_ribbon_RRN7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Refer
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